LOCUS       DAVLFB010000001        67919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLFB010000001 DAVLFB010000000
VERSION     DAVLFB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.001"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            438..1301
                     /locus_tag="VXD58_000001"
     CDS             438..1301
                     /locus_tag="VXD58_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0674771.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1587..1787
                     /locus_tag="VXD58_000002"
     CDS             1587..1787
                     /locus_tag="VXD58_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV0674772.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            1854..2519
                     /locus_tag="VXD58_000003"
     CDS             1854..2519
                     /locus_tag="VXD58_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0674773.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2591..3817
                     /locus_tag="VXD58_000004"
     CDS             2591..3817
                     /locus_tag="VXD58_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0674774.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLF
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4170..4244)
                     /locus_tag="VXD58_000005"
                     /pseudo
     CDS             complement(4170..4244)
                     /locus_tag="VXD58_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4315..4971
                     /locus_tag="VXD58_000006"
     CDS             4315..4971
                     /locus_tag="VXD58_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV0674775.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            4961..5170
                     /locus_tag="VXD58_000007"
     CDS             4961..5170
                     /locus_tag="VXD58_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV0674776.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            5388..6218
                     /locus_tag="VXD58_000008"
     CDS             5388..6218
                     /locus_tag="VXD58_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0674777.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6372..7226
                     /locus_tag="VXD58_000009"
     CDS             6372..7226
                     /locus_tag="VXD58_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0674778.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7326..8699
                     /locus_tag="VXD58_000010"
     CDS             7326..8699
                     /locus_tag="VXD58_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV0674779.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            8692..9753
                     /locus_tag="VXD58_000011"
     CDS             8692..9753
                     /locus_tag="VXD58_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV0674780.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            9755..10447
                     /locus_tag="VXD58_000012"
     CDS             9755..10447
                     /locus_tag="VXD58_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0674781.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(10476..11045)
                     /locus_tag="VXD58_000013"
     CDS             complement(10476..11045)
                     /locus_tag="VXD58_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0674782.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11180..11794
                     /locus_tag="VXD58_000014"
     CDS             11180..11794
                     /locus_tag="VXD58_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0674783.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            11796..13442
                     /locus_tag="VXD58_000015"
     CDS             11796..13442
                     /locus_tag="VXD58_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0674784.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            13454..14740
                     /locus_tag="VXD58_000016"
     CDS             13454..14740
                     /locus_tag="VXD58_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0674785.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            14792..15325
                     /locus_tag="VXD58_000017"
     CDS             14792..15325
                     /locus_tag="VXD58_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0674786.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            15403..15873
                     /gene="nrdI"
                     /locus_tag="VXD58_000018"
     CDS             15403..15873
                     /gene="nrdI"
                     /locus_tag="VXD58_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV0674787.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16166..17428)
                     /locus_tag="VXD58_000019"
     CDS             complement(16166..17428)
                     /locus_tag="VXD58_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV0674788.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(17738..18196)
                     /locus_tag="VXD58_000020"
     CDS             complement(17738..18196)
                     /locus_tag="VXD58_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV0674789.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18193..18633)
                     /locus_tag="VXD58_000021"
     CDS             complement(18193..18633)
                     /locus_tag="VXD58_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0674790.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            18989..20938
                     /gene="mutL"
                     /locus_tag="VXD58_000022"
     CDS             18989..20938
                     /gene="mutL"
                     /locus_tag="VXD58_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV0674791.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21263..21730)
                     /locus_tag="VXD58_000023"
     CDS             complement(21263..21730)
                     /locus_tag="VXD58_000023"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV0674792.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(21731..22936)
                     /locus_tag="VXD58_000024"
     CDS             complement(21731..22936)
                     /locus_tag="VXD58_000024"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV0674793.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(22956..23591)
                     /gene="ribE"
                     /locus_tag="VXD58_000025"
     CDS             complement(22956..23591)
                     /gene="ribE"
                     /locus_tag="VXD58_000025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV0674794.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(23576..24676)
                     /gene="ribD"
                     /locus_tag="VXD58_000026"
     CDS             complement(23576..24676)
                     /gene="ribD"
                     /locus_tag="VXD58_000026"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV0674795.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(24811..24925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25082..25675
                     /gene="ruvA"
                     /locus_tag="VXD58_000027"
     CDS             25082..25675
                     /gene="ruvA"
                     /locus_tag="VXD58_000027"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV0674796.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            25685..26248
                     /locus_tag="VXD58_000028"
     CDS             25685..26248
                     /locus_tag="VXD58_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV0674797.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26245..26922
                     /locus_tag="VXD58_000029"
     CDS             26245..26922
                     /locus_tag="VXD58_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0674798.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(27059..28090)
                     /locus_tag="VXD58_000030"
     CDS             complement(27059..28090)
                     /locus_tag="VXD58_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV0674799.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(28345..29028)
                     /locus_tag="VXD58_000031"
     CDS             complement(28345..29028)
                     /locus_tag="VXD58_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV0674800.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29040..29984)
                     /locus_tag="VXD58_000032"
     CDS             complement(29040..29984)
                     /locus_tag="VXD58_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0674801.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30116..32947
                     /gene="uvrA"
                     /locus_tag="VXD58_000033"
     CDS             30116..32947
                     /gene="uvrA"
                     /locus_tag="VXD58_000033"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV0674802.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            32940..34001
                     /locus_tag="VXD58_000034"
     CDS             32940..34001
                     /locus_tag="VXD58_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0674803.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            34133..34531
                     /gene="spx"
                     /locus_tag="VXD58_000035"
     CDS             34133..34531
                     /gene="spx"
                     /locus_tag="VXD58_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV0674804.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            34597..35166
                     /locus_tag="VXD58_000036"
     CDS             34597..35166
                     /locus_tag="VXD58_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0674805.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            35253..35519
                     /locus_tag="VXD58_000037"
     CDS             35253..35519
                     /locus_tag="VXD58_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV0674806.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            35523..35942
                     /gene="ruvX"
                     /locus_tag="VXD58_000038"
     CDS             35523..35942
                     /gene="ruvX"
                     /locus_tag="VXD58_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV0674807.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            35958..36263
                     /locus_tag="VXD58_000039"
     CDS             35958..36263
                     /locus_tag="VXD58_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV0674808.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            36521..37771
                     /locus_tag="VXD58_000040"
     CDS             36521..37771
                     /locus_tag="VXD58_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0674809.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            37768..38310
                     /locus_tag="VXD58_000041"
     CDS             37768..38310
                     /locus_tag="VXD58_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV0674810.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            38456..39988
                     /gene="cls"
                     /locus_tag="VXD58_000042"
     CDS             38456..39988
                     /gene="cls"
                     /locus_tag="VXD58_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV0674811.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            40246..41784
                     /locus_tag="VXD58_000043"
     CDS             40246..41784
                     /locus_tag="VXD58_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV0674812.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            41904..44111
                     /gene="nrdD"
                     /locus_tag="VXD58_000044"
     CDS             41904..44111
                     /gene="nrdD"
                     /locus_tag="VXD58_000044"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV0674813.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            44123..44263
                     /locus_tag="VXD58_000045"
     CDS             44123..44263
                     /locus_tag="VXD58_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674814.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            44307..44813
                     /locus_tag="VXD58_000046"
     CDS             44307..44813
                     /locus_tag="VXD58_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0674815.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            44806..45396
                     /gene="nrdG"
                     /locus_tag="VXD58_000047"
     CDS             44806..45396
                     /gene="nrdG"
                     /locus_tag="VXD58_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV0674816.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            45404..46021
                     /locus_tag="VXD58_000048"
     CDS             45404..46021
                     /locus_tag="VXD58_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV0674817.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRYASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            46287..46595
                     /gene="rpsJ"
                     /locus_tag="VXD58_000049"
     CDS             46287..46595
                     /gene="rpsJ"
                     /locus_tag="VXD58_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV0674818.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            46812..47438
                     /gene="rplC"
                     /locus_tag="VXD58_000050"
     CDS             46812..47438
                     /gene="rplC"
                     /locus_tag="VXD58_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV0674819.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            47463..48086
                     /gene="rplD"
                     /locus_tag="VXD58_000051"
     CDS             47463..48086
                     /gene="rplD"
                     /locus_tag="VXD58_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV0674820.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            48086..48382
                     /locus_tag="VXD58_000052"
     CDS             48086..48382
                     /locus_tag="VXD58_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV0674821.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            48400..49233
                     /gene="rplB"
                     /locus_tag="VXD58_000053"
     CDS             48400..49233
                     /gene="rplB"
                     /locus_tag="VXD58_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV0674822.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            49337..49618
                     /gene="rpsS"
                     /locus_tag="VXD58_000054"
     CDS             49337..49618
                     /gene="rpsS"
                     /locus_tag="VXD58_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV0674823.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            49630..49974
                     /gene="rplV"
                     /locus_tag="VXD58_000055"
     CDS             49630..49974
                     /gene="rplV"
                     /locus_tag="VXD58_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV0674824.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            49987..50640
                     /gene="rpsC"
                     /locus_tag="VXD58_000056"
     CDS             49987..50640
                     /gene="rpsC"
                     /locus_tag="VXD58_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV0674825.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            50644..51057
                     /gene="rplP"
                     /locus_tag="VXD58_000057"
     CDS             50644..51057
                     /gene="rplP"
                     /locus_tag="VXD58_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV0674826.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            51067..51273
                     /locus_tag="VXD58_000058"
     CDS             51067..51273
                     /locus_tag="VXD58_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV0674827.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            51298..51558
                     /gene="rpsQ"
                     /locus_tag="VXD58_000059"
     CDS             51298..51558
                     /gene="rpsQ"
                     /locus_tag="VXD58_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV0674828.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            51584..51952
                     /gene="rplN"
                     /locus_tag="VXD58_000060"
     CDS             51584..51952
                     /gene="rplN"
                     /locus_tag="VXD58_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV0674829.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            52030..52335
                     /gene="rplX"
                     /locus_tag="VXD58_000061"
     CDS             52030..52335
                     /gene="rplX"
                     /locus_tag="VXD58_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV0674830.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            52359..52901
                     /gene="rplE"
                     /locus_tag="VXD58_000062"
     CDS             52359..52901
                     /gene="rplE"
                     /locus_tag="VXD58_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV0674831.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            52919..53188
                     /gene="rpsN"
                     /locus_tag="VXD58_000063"
     CDS             52919..53188
                     /gene="rpsN"
                     /locus_tag="VXD58_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV0674832.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            53473..53871
                     /gene="rpsH"
                     /locus_tag="VXD58_000064"
     CDS             53473..53871
                     /gene="rpsH"
                     /locus_tag="VXD58_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV0674833.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            54063..54599
                     /gene="rplF"
                     /locus_tag="VXD58_000065"
     CDS             54063..54599
                     /gene="rplF"
                     /locus_tag="VXD58_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV0674834.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            54683..55039
                     /gene="rplR"
                     /locus_tag="VXD58_000066"
     CDS             54683..55039
                     /gene="rplR"
                     /locus_tag="VXD58_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV0674835.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            55057..55551
                     /gene="rpsE"
                     /locus_tag="VXD58_000067"
     CDS             55057..55551
                     /gene="rpsE"
                     /locus_tag="VXD58_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV0674836.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            55523..55747
                     /gene="rpmD"
                     /locus_tag="VXD58_000068"
     CDS             55523..55747
                     /gene="rpmD"
                     /locus_tag="VXD58_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV0674837.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            55892..56332
                     /gene="rplO"
                     /locus_tag="VXD58_000069"
     CDS             55892..56332
                     /gene="rplO"
                     /locus_tag="VXD58_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV0674838.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            56345..57655
                     /gene="secY"
                     /locus_tag="VXD58_000070"
     CDS             56345..57655
                     /gene="secY"
                     /locus_tag="VXD58_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV0674839.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            57806..58444
                     /locus_tag="VXD58_000071"
     CDS             57806..58444
                     /locus_tag="VXD58_000071"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV0674840.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            58561..58779
                     /gene="infA"
                     /locus_tag="VXD58_000072"
     CDS             58561..58779
                     /gene="infA"
                     /locus_tag="VXD58_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV0674841.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            58804..58920
                     /gene="rpmJ"
                     /locus_tag="VXD58_000073"
     CDS             58804..58920
                     /gene="rpmJ"
                     /locus_tag="VXD58_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV0674842.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            58938..59303
                     /gene="rpsM"
                     /locus_tag="VXD58_000074"
     CDS             58938..59303
                     /gene="rpsM"
                     /locus_tag="VXD58_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV0674843.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            59321..59704
                     /gene="rpsK"
                     /locus_tag="VXD58_000075"
     CDS             59321..59704
                     /gene="rpsK"
                     /locus_tag="VXD58_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV0674844.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59747..60682
                     /locus_tag="VXD58_000076"
     CDS             59747..60682
                     /locus_tag="VXD58_000076"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV0674845.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            60694..61080
                     /gene="rplQ"
                     /locus_tag="VXD58_000077"
     CDS             60694..61080
                     /gene="rplQ"
                     /locus_tag="VXD58_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV0674846.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            61345..61611
                     /locus_tag="VXD58_000078"
     CDS             61345..61611
                     /locus_tag="VXD58_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV0674847.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            61621..62958
                     /locus_tag="VXD58_000079"
     CDS             61621..62958
                     /locus_tag="VXD58_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV0674848.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            63202..63894
                     /locus_tag="VXD58_000080"
     CDS             63202..63894
                     /locus_tag="VXD58_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0674849.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(63997..65633)
                     /locus_tag="VXD58_000081"
                     /pseudo
     CDS             complement(63997..65633)
                     /locus_tag="VXD58_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(65623..66651)
                     /locus_tag="VXD58_000082"
     CDS             complement(65623..66651)
                     /locus_tag="VXD58_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0674850.1"
                     /translation="MNITIPKGSLFTLLGPSGCGKTTLLRMIAGFNSIKDGEFYFDDT
                     KINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLKQKKVPKEELIQQTNKYLELMQI
                     AQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEPLSNLEAKLRLDMRQAIREIQH
                     EVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNI
                     IPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISD
                     SVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNRIRLRINTQKLNIFSADGSQNL
                     IKGVNHGM"
     gene            complement(66726..67832)
                     /locus_tag="VXD58_000083"
                     /pseudo
     CDS             complement(66726..67832)
                     /locus_tag="VXD58_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        20261 a        12729 c        14881 g        20048 t
ORIGIN      
        1 aaagataaag tttacacaag ggggatctgg ataagagatt aactattgat attaaaaagg
       61 caataattta taaaatttgt acaaaaaaat gttaaatttt cacattttca tagtatactg
      121 tttaatagaa agtaggttct ttgtaaccgc tccattataa aatcctttca ctcttccggt
      181 atactattag gaaaaacata actggaggat attttgagct cacacctcat cgttccagtg
      241 gctgttcccc aagctcgtgt actgcccaaa aaagttagac attctattta gaacgaggat
      301 tgagttctgt attgtacaag gctcggtcct tttagagtca gcttaaggct ggctttttta
      361 ttttttctaa ctatcagaat attttgacaa atgaacacaa ataatgatat aatatgcaaa
      421 agctaggagg tggtaggatg attgaaaaaa tggaattggg ggaattttac aaggaattac
      481 gtttggcgag aaagctcaag cagtcagatg tggcttgtga cgggctaaca gcttctcagt
      541 tgtccaagtt tgagctaggg cagtccatgt tgtctgctga taagctcata ttagccatcc
      601 aagggatcaa tgtgaccttt gatgagtttg ggcacaagct caacaactat caagaatctc
      661 cacatatgca gattggtaga aaggttgtgg atcgctttgc tcatcaagat atagctggct
      721 tagagcaact gttggaggaa gtcgagcaag aacagatggc agagacttat cgtcgtttga
      781 atgctattgt aatcaaagat gccatccact cactagataa aagctaccca ctagcagagg
      841 aggatagcga gtttttgacc acctatctct atgctatcga gtcttggacc tggtttgaac
      901 tctatctctt ttgcaatact ctgcccttct tgagcaatca agatctgatc tttttatcaa
      961 cctccttact cgaaaaatcc aaagaattta aagagttagt acacaatcga ttgtatatga
     1021 agcaaggact cttaaatatc atctcagagc tcatggagcg caaacttttt tcttatatcc
     1081 caatctttga agccgagctg gagagtatgc tccgtccata cgatgttttt gagaaagtgt
     1141 tgtggcagtt tttaaagaag atgagcattt tccttcaaac taagggaagc aatcaaaaag
     1201 agattgaaca ctttatccaa tctctgcaag tattagaaaa tccacaatta atagcccttt
     1261 ttgaattgcg tttgcagcaa tacaaaaagc ttatcgatta gttagaaatg gcaaaataat
     1321 aataatctca gcaacgattt tcaataatgt taaaatttgt ttcttcatat ttctccactc
     1381 ctcattagta gtaactttat tataccagag aagtaatcaa attcaccaaa atcgcaaatc
     1441 tgaaatttct ataagaaaaa atatcaaata tgcgattttc taaaataagc caattttcgt
     1501 gttatactgt acttgtaaag cacttgaagc aaatcctagg tcacagaagt ggtttacaaa
     1561 tgaagataat tgaaggagtg taaaagatgt taaatttaca atttgcagaa acaatggaat
     1621 tgacagaagc tgagttggag atagtttatg gaggggaatt tgggaataat gctgttatcc
     1681 cagccggtgc ttggggaggg ttcgggacac cttggtcaat cactaatttc tggaagaaaa
     1741 attttaacga tagacctgac tttgattctg accgccgtcg ttattaatat ccaaagccct
     1801 tgccttttct tgggtagggg ctgtctttat ttcatagtat aggaggaaat agaatgaaaa
     1861 agataatctc acaccgctac tttattatag tttttctact agttattgct gaccagaagt
     1921 tcagtgttct agttttacgc agcgaccttg ttactgggct atctgacttt gcctattatc
     1981 tgtcggatat gatgttgaat tttcttgtgg ttttatttgc tcttattgct atgatttggt
     2041 cggggaaatg gcagaaaatc aatagtagaa agtttaaagg atcctatctt ttctattcct
     2101 tcctagctct gcttgctttt gttgtttgga atttcgttac cttttttctt ttcccaccta
     2161 ctcgaaatga aatttcttat caacatgctg ctccgacttt tacaggggct acagcatttt
     2221 tgatgtattt tttctatcct gtgattgcag gtcccatttt tgaagagata atccatcggg
     2281 gattggtgat gacagctctg gaaaaaggaa agaaatgggg gctagatgta cttggctctg
     2341 ctattttatt tggggtctcg cacattagta atcacggttg ggtcttgaca gactttgtct
     2401 tctatatggg tggaggtctt atatttgcag tcttatttag aatgacaaag tcaatttatt
     2461 ggcctattgg actgcatata gtctacaatg gtattggtca gcttttgatg ttactgtaat
     2521 tttggttgtt agtagtatcg cggtctattc ttggggaaag attcccattt gaacttaaga
     2581 aggagttgta atgaaagtat ttcttcaaaa tagagatttt aggcaattaa ctatcaacca
     2641 gtggatttca acgcttgggg atacgatttt ttatctggcc ttcctgaatt atgtggcaga
     2701 tgcgtctttt gcccctttgg cgattttact tatcacgatt tcagaaaccc ttccccaagt
     2761 tctacaaatc tttctgggag ttttggcgga ttttcaacat catcgtgtct tgaaatacac
     2821 agtcattagt tttacaaaat ttttgcttta ctctatagtg tccctatcgc tttcagggca
     2881 gtctttttcc ttgtttctag taacctttat ttgtatgatt aacctcttgt ctgacacatt
     2941 gagttatttt tcaggcgcca tgctcactcc gattttcatt agaattattg ggcaagatca
     3001 tctggcagaa gctattggat ttaaacagtc aactgttagt ttagtgaaaa caatcagtaa
     3061 tatcctagga ggagtcttac taggtattct atccatccag tttatttcct tactgaacgc
     3121 tcttaccttt ttaatcgcat ttttaggtat cctcttcata aaaactgacc tcttgaaagt
     3181 agaaaaaacg attagctatc aagaaggact ctctgtaaaa tccttttgcc agcatttgct
     3241 ccaatcatcg aaattgatat ggaatatgaa taaggtgctc ttggttttgt ttattatctc
     3301 tactagtcaa gcagtgataa atgttacagt tcctgtctct actctatttt tgaggaatca
     3361 accctttttg aatttacaaa caggtcaatc tcttgccttg ctatccactt ttgaactgtc
     3421 agccctgatt gtcggaagcc ttgtaagtgg ctatctgcaa cataccattt ccataaaaac
     3481 agctctctat gcctcacttg tcatccagtt gcttcttcta gtaggatttg ccacagttcg
     3541 ttttgactgg attctcatct ttagtacctt ggatgccttt ttcgcaggtg ttctctctcc
     3601 tagattgcaa gaactcgtct ttaaacaaat acctgaggag tcaatgggag cagttcaatc
     3661 ttctatcagt gccattacgg ttgttttacc tagcttattt acaatatctt tggtaaccat
     3721 tgctactagc tttggaactc tggcagttag ctttgtttta ctgctatttc ttctagttgc
     3781 ctttgttatg cttctgaata ttcgtgaaag tatttagtgg agaatttcta taatatatgt
     3841 ttgagactgg attgccagtc tttttatgat ggtcaaaatc aacagacttt ttctcttttc
     3901 tgaatgtgtt atagtagact gaatctaaaa tagtacgaaa caattgctaa aacatttata
     3961 gaaattaatt ttactttccc aatcgatttg ttctcatctt atttcaatct gctatataat
     4021 agtccatgct gtggctggat tcttttcagc ctacttatac tcttcgaaac tctcttcaaa
     4081 ccacgtcagc ttcaccttgc cgtatatgtg ttactgactt cgtcagttct atccacaacc
     4141 tcaaaacagt gttttgagct gacttcatca gttctatctg caacctcaaa acagtgtttt
     4201 gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta ttattaagac
     4261 aatatgagga gaaagatgaa agaaaaagga ttttaggagg gggcgcaggc agccatgcca
     4321 acggctcttg gctatgtcag tatcggcctg gcctgtggaa ttatcggtgc gccctatgtg
     4381 acacctgttg agatgggctt gatgagtctc tttgtttatg ctgggagtgc ccagtttgcc
     4441 atgttggcac tgattgtggt tcaagctcct gtggcagcta ttgctatgac ggtttttcta
     4501 atcaatttgc gtctcttttt gttgagctta cacgcatcga cttatttccg tcataccagt
     4561 ctttggcaca atatcggtat gtctagtatc ttgacagatg agacctatgg cgttttgatg
     4621 ggcgaattag cccatacaga taaggtcaat ccgatgtgga tgcacgggaa caatctcaac
     4681 agctatgtgg cttggtttgt gggaacagtg gtcggaacgg ctctaggtga cctgttgcca
     4741 aatccagaaa tctttggttt agattttgcc ctggttggga tgtttatcgg gatttttgct
     4801 tcgcaattcc agatgatgca aagacggatt tctgtccgca atctgctcat tatcttagca
     4861 gttgttgcag tgtccttctt tttacttttg acagtggtgt ctcagtcact agctgttctg
     4921 tttgcgacgc tacttggttg tagcatgggg atggttttag atggtcagta agtatctttt
     4981 attagcagtt attttctctg gtcttgtgac ctggattccc cgtatgattc ccttcatctt
     5041 ggtcaagtat aagggcctgc ctacgattgt tgagcgtttt ttgaagtttt tgcctgtttc
     5101 cattatcttt gccttgattc tttcaagagt agtgacaggt aaggttggga accttcctca
     5161 aatcaaatga ctggacttct tagcagtctt tccaacagct tgggtagctt ttcgctaccg
     5221 caatctagtc ggtacggttc tctttggagt agtcttgatt gccgtcttgc gtttggtctt
     5281 ttaaataagt cataaagaaa acttatcaca gagatagata tcatataatg gcgtaattac
     5341 tttttttctg ataagattat aaggtattct attttggagg aaatgacatg aaaaaaatcg
     5401 ttaaatactc atctcttgca gcccttgctc ttgttgctgc aggtgtgctt gcggcttgct
     5461 caggtggtgc taagaaagaa ggacaagcag ctagcaagaa agaaatcatc gttgcaacca
     5521 atggatcacc aaggccattt atctatgaag aaaatggcga attgactggt tacgagattg
     5581 aagtcgttcg cgctatcttt aaagattctg acaaatatga tgtcaagttt gaaaagacag
     5641 aatggtcagg tgtctttgct ggtcttgacg ctgaccgtta caatatggct gtcaacaatc
     5701 ttagctacac taaagaacgt gcggagaaat acctctatgc cgcaccaatt gcccaaaatc
     5761 ctaatgtcct tgtcgtgaag aaagatgact ctagtatcaa gtctctcgat gatatcggtg
     5821 gaaaatcgac ggaagtcgtt caagccacta catcagctaa gcagttagaa gcatacaacg
     5881 ctgaacacac ggacaaccca actatcctta actatactaa ggcagatttc caacaaatca
     5941 tggtacgttt gagcgatgga caatttgact ataagatttt tgataaaatc ggtgttgaaa
     6001 cagtgatcaa gaaccaaggt ttggacaact tgaaagttat cgaacttcca agcgaccaac
     6061 aaccgtacgt ttacccactt cttgctcagg gtcaagatga gttgaaatcg tttgtagaca
     6121 aacgcatcaa agaactttat aaagatggaa ctcttgaaaa attgtctaaa caattcttcg
     6181 gagacactta tctaccggca gaagctgata ttaaataatt tcttgaaatc atccattctg
     6241 agtaagatgg gtggtttctc agtttttagg gatatagttt taaactatat atctgactat
     6301 ctaataacaa tttgtgaaat caaacaaatt gtgagatact agtacggtat tatttttaag
     6361 gagaaagaat catgaaaatc aaaaaatggc ttggtctagc agcccttgct acagtcgcag
     6421 gtttggctct tgcagcttgc ggaaactcag aaaagaaagc agacaatgca acaactatca
     6481 aaatcgcaac tgttaaccgt agcggttctg aagaaaaacg ttgggacaaa atccaagaat
     6541 tggttaaaaa agacggaatt accttggaat ttacagagtt cacagactac tcacaaccaa
     6601 acaaagcaac tgctgatggc gaagtagatt tgaacgcttt ccaacactat aacttcttga
     6661 ataactggaa caaagaaaac ggaaaagatc ttgtagcgat tgcagatact tacatctctc
     6721 caatccgcct ttactcaggt ttgaatggaa gtgccaacaa gtacactaaa gtagaagaca
     6781 tcccagcaaa cggagaaatc gctgtaccaa atgacgctac aaacgaaagc cgtgcgcttt
     6841 acttgcttca atcagctggc ttgattaaat tggatgtttc tggaactgct cttgcaacag
     6901 ttgccaacat caaagaaaat ccaaagaact tgaaaatcac tgaattggac gctagccaaa
     6961 cagctcgttc attgtcatca gttgacgctg ccgttgtaaa caataccttc gttacagaag
     7021 caaaattgga ctacaagaaa gcacttttca aagaacaagc tgatgaaaac tcaaaacaat
     7081 ggtacaacat cattgttgcg aaaaaagatt gggaaacatc acctaaggct gatgctatca
     7141 agaaagtaat cgcagcttac cacacagatg acgtgaaaaa agttatcgaa gaatcatcag
     7201 atggtttgga tcaaccagtt tggtaataag aaacagggag gtgggagaga aaattccacc
     7261 tcttgctttt gtatagagta tagattgtaa agaagactat tcgttcatag aaaggtagag
     7321 agaatatggt ttttcctagc gaacaagaac agattgaaaa atttgaaaag gatcatgtag
     7381 tccaacatta ttttgaggtt ttgcgtacct tgatttctaa gaaatcagtc tttgcccagc
     7441 aggttggact caaggaagtc gcaaattatc tgggtgagat tttcaagcgt gttggagctg
     7501 aagtggagat tgatgagagc tatacagcgc cctttgtcat ggcacatttc aagagttcgc
     7561 gtccagatgc caagaccttg attttctata accactatga cactgtgcca gcggatgggg
     7621 atcaggtctg gacagaggat ccttttacgc tttcggtccg caatggcttc atgtatgggc
     7681 gtggggttga tgacgacaag ggtcatatca cagctcgctt gagtgctttg agaaaatata
     7741 tgcagcacca tgatgattta cctgtcaata tcagctttat catggaggga gcggaggaat
     7801 cggcttcaac agacctagat aagtatttgg aaaagcatgc agacaaactc cgtggggcgg
     7861 atttgttggt ctgggaacaa gggaccaaaa atgccttgga gcagctggaa atttctggtg
     7921 gcaataaggg gattgtgacc tttgatgcca aggtaaaaag cgctgatgtg gatatccact
     7981 cgagttatgg tggtgttgtg gaatcagctc cttggtatct cctccaagcc ttacagtctc
     8041 ttcgtgctgc ggatggccgt atcttggttg aaggcttgta cgaagaagta caagagccca
     8101 atgaacgaga aatggccttg ctagaaactt atggtcaacg aaacccagag gaagttagtc
     8161 ggatttatgg attggagttg cctctcttac aggaggagcg gatggccttt ctaaaacgtt
     8221 tctttttcga gccagcgctt aatatcgaag gaatccagtc tggttatcaa ggtcagggtg
     8281 ttaagactat tttacctgca gaagccagtg ccaagctaga ggttcgtctg gttccgggcc
     8341 tagaaccgca tgatgttctg gaaaaaattc ggaaacagct agacaaaaat ggctttgata
     8401 aggtagaatt atactatacc ttgggagaga tgagctatcg aagcgatatg agcgcaccag
     8461 ccattctcaa tgtgatcgag ttggccaaga aattctatcc acagggcgtt tcagtcttgc
     8521 cgacgacagc ggggacagga cctatgcata cggtctttga tgccctagag gtaccaatgg
     8581 ttgcattcgg tctaggaaat gccaatagcc gagaccacgg tggagatgaa aatgtgcgaa
     8641 tcgctgatta tcacacccat atcgaattag tagaggagct gattagaagc tatgagtaga
     8701 gatattatca agttagatca gatcgatgtg acttttcacc aaaagaagag aaccattaca
     8761 gcggttaagg atgtgaccat tcacatccaa gaaggggata tctacggaat cgttggatat
     8821 tctggagcag gaaaatcaac ccttgtacgg gtgattaatc tcttgcaaaa accatctgca
     8881 gggaaaatta ccattgacga cgatgtgatt tttgacggca aggtgacctt gacggcagag
     8941 cagttgcgtc gtaaacgtca agatatcgga atgattttcc agcattttaa cctgatgagc
     9001 caaaagacag cagaggagaa tgtagccttt gcccttaaac actctgaact cagcaaggaa
     9061 gaaaagaagg ctaaagtagc taagttgttg gacttggttg gtttggcaga tcgtgctgaa
     9121 aactaccctt cacaactatc tggagggcaa aaacagcgtg tggcaattgc gcgtgccttg
     9181 gccaatgatc caaaaatctt gatttcagac gagtcaactt ctgcccttga tccgaagaca
     9241 accaagcaga ttttggcctt gttgcaagat ttgaaccaaa aattaggctt gactgttgtc
     9301 ttgattacgc atgaaatgca gattgtcaaa gacattgcca accgtgtggc ggttatgcag
     9361 gatgggcatt tgattgaaga gggtagtgtg cttgaaatct tctcagaccc taaacaacct
     9421 ttgactcaag actttatctc aacagctaca ggtattgacg aagccatggt caaaatcgag
     9481 aagcaagaaa tcgtggaaca cttgtctgaa aacagtctct tggtgcaact caagtacgct
     9541 ggatcttcaa cagacgagcc acttttgaat gaattgtaca agcattatca agtaatggct
     9601 aatattctct atgggaatat cgaaatcctc gatggtactc ctgttggaga attggtggtg
     9661 gtcttgtcag gtgaaaaagc agcgttggca ggtgcccaag aagccattcg tcaagcaggc
     9721 gtacagttaa aagtattgaa gggaggacag taagatggaa tcattgattc aaacctattt
     9781 gccaaatgtc tataagatgg gttggtctgg tcaggcaggc tggggaacag ctatctacct
     9841 aaccctctat atgacagttc tttccttcat tatcggaggc ttcttggggc tagtggcagg
     9901 tctctttctc gtcttgacag cgccaggtgg tgtcttggag aataaagtcg tattctggat
     9961 tttagacaaa attacctcaa tttttcgtgc gattcccttt atcatcctct tggcaatctt
    10021 gtcaccactt tctcacttga ttgttaaaac aagtatcggg ccaaatgcag cccttgtccc
    10081 actttctttt gcagtctttg ccttctttgc ccgtcaggtg caggttgtct tggctgaatt
    10141 ggatggcggt gtcattgagg cggctcaagc gagcggagcg actttctggg acatcgtggg
    10201 tgtttaccta tcagaaggtc ttccagattt gatccgtgtg acgactgtga ccttgatttc
    10261 ccttgttggg gaaacagcta tggccggtgc ggttggagct ggtggtatcg gtaacgtagc
    10321 catcgcttat ggatttaacc gctacaatca cgatgtgacc atcttggcaa ccattattat
    10381 cattttgatt atctttgcaa tccaattctt gggagatttc ttgactaaga aattgagtca
    10441 taaataaaaa gagccgcgtg gctcttttta actgatcaga ttttctgtgc aaatttttta
    10501 ctcaaggctt gtccaatcaa ggcacccact agggctccga tgacaatact tgcgataaat
    10561 agaaggacag ttccagggtt tggagcgacc atgatacggt cgatatattc ttgggatttt
    10621 cctcttgcca gaagagtagc catataggct ttgggcgcaa tccacataag caagattggt
    10681 cctgttgtac taaaggcgaa aataatgaaa gaaaggaagt tctttgtttt gtccttgtat
    10741 tttcctaaat gagctacttc atctgctagg aggccacaga taattccagg aaggaaggaa
    10801 ccagcaccgt gtttagttcc caagaaaaag agggcaatga caaggccgat agtggtaatg
    10861 gctccaaagc gcggaacttt tgcgactagg atcatataga cgctaccgcc gacaagggca
    10921 gtaaaggcag gcgcataaaa catgtttcca gtttggtcaa agagattgcc caaaaggaca
    10981 ccaatcccca tgcagaggaa gtaaagaact gcaaaaagca gtgtagttaa gatagatttc
    11041 ttcatgaatt gtctcctgat aattttttca caattctcat tgtaccacgg tttggtggga
    11101 ttgtaaatgg atactagaag tttttgaaaa cgcttgaaat atgatataat ggtttcatag
    11161 ttatttttag gggaatatca tgggtagatt tttagacttt gtctttaatc gtttcttttt
    11221 agggatgatt gcgacagcct tcttttggct attaacttta gcaggaggga ttatccttgg
    11281 tctagcgccg gctagtgcca ccttgatgag cttatatgca gaacatggtt atagctttcg
    11341 ggaatacagt ttgaaggagg cttggtctct ttacaagcaa aattttgtct caagcaacct
    11401 gattttctat agctttttag gtgtgggtct agttttgacc tatggtttgt atctcttggt
    11461 gcaattgcct catcagacca ttgttcattt gattgcgacc cttttgaatg tcctagtagt
    11521 tgccctgatc tttttggctt atacagtatc tttaaaatta caagtttatt ttgccttgtc
    11581 ctatcgaaat agtctcaaat tatccttgat tggcatcttt atgagtctag cagctgtggc
    11641 caagattctc cttgggactg tgctacttgt agcaattggt tactatatgc ctgccctgct
    11701 attttttgta ggaattggga tgtggcattt ctttatcagt gatatgttgg aacctgtcta
    11761 tgaaatcatc catgaaaaat tggcgacaaa atagaatgaa gcagttttgg ctacatacgc
    11821 ttctaagaac ctatagttca gtgatgatca ttatcattgc gagttttgca atcttactct
    11881 cttacgctga ctgggattca cgtgaaaagg aagcccagag agtagcccag cgtgtaactg
    11941 ctagaacagt gagtgaaatt gaatattacc atagagagtc aacccagata gctcaggctt
    12001 tagttgaaaa ccaagctcgt attgagggaa tctataaata ctttagtctt agcatgccag
    12061 actattttta ctggcaatta gagcggaaag cttcgcctta tatatcagtc tctctgtatg
    12121 aaaatgttga tgacctctat gttcgaaatg attttgtaac tggagtggcc attgcttttc
    12181 aagattacaa ggaagtctat gtttctacta aagacaaacg tagtggagaa aaaatcaggg
    12241 ctgaggattt caaaccagca ggaaatagtt ttgccattcc ggtgtcagat ccagtgtcag
    12301 atcaagattt aggagtgatt tacatctcct tggatcctgc tgttttatac catgccattg
    12361 ataatactag aggtcatact ccgatggcag taacagtgac ctcacctttt gatacggaga
    12421 tttttcatat tggtgagaca gttgataagg agagtgaaaa ttggctagtt ggcttaactt
    12481 ctcatggata tcaggttcag gtggcagttc ctaaaaactt tgttttacaa ggaacagtga
    12541 ctagctctgc tttgattgtg ggtttgagcc ttctctttat tgtcattctt tatctgactt
    12601 tgaggcagac ttttgctaat taccaaaagc aggtagtgga tttagtagaa tccattcaag
    12661 tcattgctca aggcgaagag gggcgtcgga ttgacatttc cgagaaagat caggaattac
    12721 tcctaatcgc ggagacgacc aatgatatgt tggatcgatt ggaaaagaat atccatgata
    12781 tttaccagtt agagcttagt caaaaagatg ccaatatgcg agccttgcag gcgcaaatca
    12841 atcctcattt tatgtataat acgctggagt tcttgcgcat gtatgcagtt atgcagagtc
    12901 aagatgagtt ggcagatatc atttatgaat tcagtagtct cttgcgtaac aatatttccg
    12961 acgaaagaga gaccctcctc aaacaggaat tagaattttg ccgtaaatac agctatctct
    13021 gcatggttcg ctatcccaag tccattgcct atggtttcaa gatagatcca gagttagaga
    13081 atatgaagat tcccaagttt accttgcaac cgctggtaga aaactatttc gcgcatggtg
    13141 ttgaccacag gcggacagat aatgtgatta gcatcaaggc tcttaaacag gatggttttg
    13201 tggaaatttt ggtggtcgat aatggtagag gaatgtcggc tgaaaagttg gcaaatatcc
    13261 gagaaaaatt aagtcagaga tattttgaac accaagccag ctacagtgat caaaggcagt
    13321 ctatcgggat tgtcaatgta cacgagcgtt ttgtgctcta ttttggagac cgctatgcca
    13381 ttactataga gtctgcagag caagccggtg ttcagtatcg tattacaatt caagatgagt
    13441 agaaagggag aaaatgtata aagtattatt agtagatgat gagtacatgg tgacagaagg
    13501 tctgaagcgt ttgattccct ttgataagtg ggatatggag gttgtcgcaa cagccagtca
    13561 tgccgatgaa gctctagaat atgttcagga aaatcctgtc gatgtcatca tttccgatgt
    13621 caatatgcca gacaaaacag ggcttgatat gattcgggag atgaaagaga tcttaccaga
    13681 tgctgcctat atcctgctct caggttatca ggagtttgat tatgtaaaaa gagcaatgaa
    13741 ccttagtgtg gtggactatt tggtcaagcc tgttgataag gtagagctgg gaaatctgct
    13801 ggagaagatt gcaggtcagc tcggcgagag agggaagaaa agtcagactc ttagtcaaga
    13861 attagacgag gctggatttg ttagttattt aggggataag gagaattggt ggataggtct
    13921 atccaaggaa aaacaaggtt ccttcaccat tccctactat gtcttgggcc aagactggca
    13981 gattttcatt tctggccaac ctctagatgg tttagtcgtt acaccttttg aagctcctta
    14041 tcaagaacac tttgaacgct ggaagctgaa tgctgagaaa accctctttt acggttctgt
    14101 aaatttgcag cagtctgaga gtctctttgc ctattacgaa ccgatttata gggttatcat
    14161 tcagggaaat ctcaatcaaa tcgtagaaga gttaaatctc ttggagaagg tagttcttga
    14221 aaatacgccg cgtgttccga ttactaaaca gctttttatt cagtttgtca tggatgtttt
    14281 ccatttattt gaacatctca aagctgatga cctgacagac atcgtcaaaa ccattcatgc
    14341 tattcaatcc ttcgatgaat tggtttctta tatcaaggaa actctgatca gctttttcgg
    14401 tcaataccgt atgaatgaaa atgtggtcag tgtgctggaa gtcattggtc gtgattacca
    14461 aaaagagctt tccctcaagg atatcagtaa ggctctcttt atcaatcctg tttatctagg
    14521 gcagttgatt aagcgtgaaa ccgattcgac ttttgcagag ttactaaaca aacaacgtat
    14581 taaggctgcc caacaacttt tgctttcaac tagtgacagc atcgaagata tttgttatgc
    14641 tgttggttac agtaaccttg gatatttcta taaagttttc cgaaaattgt gcggaaaatc
    14701 gccaaaagcc taccgaaaac aggtagaaac tatactataa gatttgtatt cctttacaaa
    14761 aggtgctata atattatcag gaggttctat catgaaaaag aaaccaattt atctatgggt
    14821 cttgctaatc ttgtctgccc ttatttcagt tccgtctctg tttggaattg tgagtccctt
    14881 gcctagtaaa gaagcccttc gtgctgctca gaaacaagtt gcaggggtca atgctcagca
    14941 attagaagac cagcttaatt atacctatag agtagcagaa gcatctcatt ctatttttaa
    15001 tgttgctttg attgtgctat ctactatttt agtggtcgta gcgattgtct tccttgttcg
    15061 taaaaatttg caatatgcaa actatactta cgttggctat gttttgttag ctattattgg
    15121 ttcgatttat ggctatgttg gtttacaaga cgctgtgcag ttggttcaag atgagagcat
    15181 gcgtttgaca gtgagtattg gttcaaaagc tgttagcatt ttctatatcg tcatcaatgt
    15241 tctcttccta gcccttgtct tctataagat gtggcgacaa cagaaagcct tggcagaaga
    15301 agaggaaaca gaagagctta cctaagtatt gacaaaaaag aactatcaag atacaatctt
    15361 ggtagttgtt ttttgattat agataaaatc atggaggtac atatgaagac aatttcttta
    15421 gtttatatta gtttgagtgg caacactgaa agttttgtga cgcgcttgaa agactatctc
    15481 ttgtcccagt acaaagggat tgaggttcaa aagattcata tcaaggattt ggtcaaggaa
    15541 ggcaaaaatt tctatgaaat ggaccatccc tatgtcgctt ttttgccgac ctatctcgaa
    15601 ggtgggaatg gcgtggataa cggagatgtt gagattttga cgacaccggt gggagatttt
    15661 attgcctatg gtaacaatgc tagtaagtgt tttggtgtgg ttgggtcagg aaatcgtaac
    15721 tttaataacc aatactgcct aacagccaag caatacagtc aacgctttgg cttccctgta
    15781 ttggctgact ttgaaatgcg aggcatgctg gaagatatca aacatgtcgc agctattatc
    15841 gcagatttgt atgagttgga aaaggagaat taatactcaa tgaaaatcaa agagcaaact
    15901 aggaagctag tcgcaggttg ctcaaagcac agctttgagg ttgcagatag agctgacgtg
    15961 gtttgaagag attctcgaat agtataaagt tcttataaga tgtggttgaa gttgttcaac
    16021 tgttttttga gtataaacag tctttaagaa cgtaaaaaag cataagttca ggtcttcaaa
    16081 accttgttct tatgcttttt atcatagaaa tatttgctga attttctctt gaaatgaaac
    16141 cttatcttca tctcgcgctt gtcccttacc ctgcttttcc gattgcgagt gcgtagataa
    16201 agaagatggc gaagccaaag aggaaaatca atcccgcaat ggcaaggtgt ttgagccaag
    16261 aaggaagatt tttgttttca cgtgttacca aggcgtaatt caaaagagca aagaaaggtg
    16321 ttgtcaggaa agagccaatc atggcaaagc ggagcatggt tgaaacctga ccagcgaaga
    16381 acttgataat gacgataccg atgatagcag tgatggtcat ccagatgttc aaagatttac
    16441 gattgtcctc tttttgactg attagcagtc ggagagattc ctgattaacg cgagaatagc
    16501 catcgataac agttataact gttccaaaga tacagaggaa ggcaataaag gtaatcaagt
    16561 aacgggacca ttcgccaaga acagaggcat acatgcccac gaattgagag atgtatttgg
    16621 ctgaagcagc ttcaaccgcc tgccctgtag gatactgaat cagtgctccc agtgccacaa
    16681 agaagacggc taggatagct gttccaatat aaccagtgtt aaagtcaaac agagcgtctt
    16741 ctgtgttaaa gttgacggtc tttctctttt cagctgacca aagtgaattg atggctgaaa
    16801 tttcaatagg agccggcatc catcctagga gggagacgat gaagggcaga gctgccattt
    16861 gccaaggtgt cttctcgaca aaatcagaac tgtattctgg atgcttgacc gccgcaatga
    16921 taactgcaag aacagtcgca atggttaagg cagacataat ccatttgacc atgccgtcta
    16981 aaagtttgta gcctccaaag agtagcatag cccaaatgat tgcaacgaga atgagggacc
    17041 actgagtaat gctaagtcca atcattggga aggcactggc gatgatagct gagcacagaa
    17101 tggcaacacc agccgtgttg accatagccg aaaagacatt gagaataaag aaaatccaga
    17161 gatagagttt tcctttttcg gcataacctt caaccaaagt ctttccagta tcagctgtgt
    17221 attcagcacc aaaacggaaa aatggatatt taaagacatt ggctaagatg accaagagaa
    17281 gtagagacca accgtaagaa ccgccagctt gagttgagga tacaatgtgg gaacctccaa
    17341 cagcggcagt tgccattagg attccaggtc ccattgcttt tagcttgctt gcccaggttg
    17401 attgatttaa ggaaatagct tgcgacatga taaatactcc tttttgaatt ttcagaataa
    17461 tctgtatatg caatctatat tctacaaaaa attttggaaa aatgcaagaa tattttgaaa
    17521 aaagatagaa aatttacaaa gaaaatctga aataactgtt atgatatagc agattgaaac
    17581 tagaatagta cacttttgct tctaaaacat tgttagaaat cgatttgact gtcctgattg
    17641 atttatcctg ttcttatttc atttcactat agtaaggtgc agcaggataa agcaaaaccg
    17701 agaagtttct ttctcggttt tgcttattat gaggttccta ttttctatgc tccaagagtt
    17761 gattgatgtg atctactttt ttagcctctc ttttatagag aatagcccag atgatgagat
    17821 agacaatcgc aaactcgata atgagctgga ggtagaaaat ccagtggaag gggaaccaac
    17881 cagcaagagt tgctagtggg acaaagccag ccagcataag gaagaaatga gtcagagtgg
    17941 cacgaagcat gctccagtca cggctgaata agcggttgcc aaagttgaag agcataccga
    18001 tggctgccca gataagtgtg cagtagagca agaccagggc accgtgaacc tgatgctgat
    18061 ccatcacttg gccgatgaga gagtagggat ttagtggtgc gtaggtattt ggtgcataaa
    18121 tgagtgaaaa gaggatggag aggatgaggc cgataagaac accggcagct gcatcgtgaa
    18181 atacttgttt tttcatagtt ctaatttctc cttgatggtt tttagataac ggcgtgaaga
    18241 gtaggtgaag ctttcgtttt tcaagaaaat ttctaccaga ccgtttggcg tgagcttgag
    18301 gtgagagatg gaatcgatat tgatgatttc tgattgggaa atttggataa aattgcttgg
    18361 caagagttta agaacctgat agagtcgcaa atcaatgctg taggtctgac tcgcggtttc
    18421 tgctagaacc ttccgattct cgatatagaa gcgctgaatc ttgccaatct caactagata
    18481 gacctgatca tcgatttttc ctttgatttt ttctctttgg tccagatttt ctgcgaactc
    18541 gatgactttc tggacttttt cggtttcttg aggtgcttgg acaatcagct tttcctcctc
    18601 gtaagtctca ctaatctgta gttctacttt cataaatttc tctccttgtt tttcatacaa
    18661 aattaatctc acttcttgta cacctttatt aatactcttc aaaaatctct tcaaaccacg
    18721 tcagcgtcgt cttgccgtag gatagttgtt gactttgtca gttttatcta caacctcaaa
    18781 acagtgtttt gagctgtctg cgtctagctt tctagtttgc tctttgattt ttattgagta
    18841 taccactatt ttactccctc tggcaaggga ctttgtctat gtggagggat tgggctccta
    18901 tgtggtggag tttttctgtt ctttctgaaa tatggtataa tagcactaat taatttctag
    18961 gaaaatagat acagaaaggg gctgaaagat gtctcatatt attgaattgc cagagatgct
    19021 ggcaaaccaa atcgcagctg gggaggtcat tgaacgtcct gccagtgtgg ttaaagagtt
    19081 ggtagaaaat gccattgacg cgggctctag tcagattatc attgagattg aggaagctgg
    19141 tctcaagaag gttcaaatca cggataacgg tcatggaatt gcccacgatg aggtggagtt
    19201 ggccctgcgt cgccatgcga ccagtaagat aaaaaatcaa gcagatctct ttcggattcg
    19261 gacgcttggt tttcgtggtg aagccttgcc ttctattgcg tctgttagtg tcttgactct
    19321 gttaacggcg gtggatggtg ctagtcatgg aaccaagtta gtcgcgcgtg ggggtgaagt
    19381 tgaggaagtc atcccagcga ctagtcctgt gggaaccaag gtttgtgtgg aggatctctt
    19441 tttcaacacg cctgcccgtc tcaagtatat gaagagccag caagcggagt tgtctcatat
    19501 cattgatatt gtcaaccgtc tgggcttggc ccatcctgag atttctttta gcttgattag
    19561 tgatggcaag gaaatgacgc ggacagcagg gactggtcaa ttgcgccaag caatcgcagg
    19621 gatttacggt ttggtcagtg ccaagaagat gattgaaatt gagaactctg acctagattt
    19681 cgaaatttca ggttttgtgt ctttacctga gttgactcgg gctaaccgca attatatcag
    19741 cctcttcatc aatggccgtt atatcaagaa cttcctgctc aatcgtgcta ttttggatgg
    19801 tttcggaagc aagcttatgg ttggacgttt tccactggct gtcattcaca tccatatcga
    19861 cccttatcta gcggatgtca atgtgcatcc aactaagcaa gaggtgcgga tttccaagga
    19921 aaaagaactg atgaccctgg tttcagaagc tattgcaaat agtctcaagg aacaaacctt
    19981 gattccagat gccttggaaa atcttgccaa atcgaccgtg cgcaatcgtg agaaggtgga
    20041 gcaaactatt ctcccactca aagaaaatac gctctactat gagaaaactg agccgtcaag
    20101 acctagtcaa actgaagtag ctgattatca ggtagaattg actgatgaag ggcaggattt
    20161 gaccctgttt gccaaggaaa ccttggaccg attgaccaag ccagcaaaac tgcattttgc
    20221 agagagaaag cctgctaact acgaccagct agaccatcca gagttagatc ttgctagcat
    20281 cgataaggct tatgacaaac tggagcgaga agaagcatcc agcttcccag agttggagtt
    20341 tttcggacaa atgcacggga cttatctctt tgcccaaggg cgagatggac tttacatcat
    20401 agatcagcac gctgctcagg aacgggtcaa gtacgaggag taccgtgaaa gcattggcaa
    20461 tgttgaccag agtcagcagc aactcctagt gccctatatc tttgaatttc ctgcggatga
    20521 tgccctgcgt ctcaaggaaa gaatgcctct cttagaggaa gtgggcgtct ttctagcaga
    20581 gtacggagaa aatcaattta ttctacgtga acatcctatt tggatggcag aagaagagat
    20641 tgaatcaggc atctatgaga tgtgcgacat gctccttttg accaaggaag tttctatcaa
    20701 gaaataccga gcagagctgg ctatcatgat gtcttgcaag cgatctatca aggccaatca
    20761 tcgtattgat gatcattcag ctagacaact cctctatcag ctttctcaat gtgacaatcc
    20821 ctataactgt cctcacggac gtcctgtttt ggtgcatttt accaagtcgg atatggaaaa
    20881 gatgttccga cgtattcagg aaaatcacac cagtctccgt gagttgggga aatattaaaa
    20941 gtataaaaaa gtctgggaaa aattttcaaa atcagaaaaa cgcataaaat caggtgttca
    21001 aaaaccttga ttttatgcgt tttatcatgg aaatagttac ttcatgtttt cctaattctt
    21061 ttcgaaactc tttttaaacc acgtcagttt tatcagtaat ctcaaaacag tgttttgagc
    21121 taattttgcc agttttgtct gtaacatcga agttgtgttt taccactctg cgactggttt
    21181 cctagtttgc tctatgattt tcacagagta ttaaattgcg attttgccaa gtttctttat
    21241 tcatctaaaa gtagagtctg ttctatgcgt ctaatgtacg aattaggttg accatttcaa
    21301 tagctccttg tgcacactca gaacccttat ttcctgcttt ggtaccagct cgttctatgg
    21361 cttgttcaat tgtatctgtc gttagcacac caaacataac aggaatttcg ctatttaaac
    21421 tgatttgggc gattccctta gatacctcgc tacatacata atcataatga cttgtattcc
    21481 ctctaatgac agctcccaag cagataattg catcatattt tttacttttt gccatttttg
    21541 atgcaatcag tggtatttca aaagctcctg gaacccaggc tacctctata tctttctcgt
    21601 ttacattctc tcttttgaga ttatctagtg ctccagataa taattttgaa gttataaatt
    21661 cattaaatct cgctacaaca atacctattt taatattgtt tgctactaaa ttaccttcat
    21721 aagtgttcat ttatttttcc tccatattta aaatgtgacc cattcgattt ttctttgttt
    21781 ctaaataaaa actatcgtaa ggattggctt ctatttcgat tgatattcta ctggaaatgg
    21841 taattccata tttttctaac tgttcaacct tgtcaggatt atttgtcagt aaatgaagtg
    21901 actgaagtcc cagatcttta agcatttgtg ctccaatatg atattctctt aaatcacctt
    21961 caaagcctaa tgcaagattg gcatcaagcg tatccatgcc ttgatcttgt aaatgatagg
    22021 cttttaattt attgataagt ccaattcctc gtccctcctg tcgcaagtaa agtaagacac
    22081 ccgaaccatt ctcaacaatc attttcatag ctttatcgaa ttgctgtcca caatcgcaac
    22141 gtaaagagcc taaaacatct cctgttaaac attcggagtg gacccgacat aatacattgg
    22201 cttcatcctc tatgtttccc ataataagag caagatgatg ttccccattt agtttatcta
    22261 tatagctaat tgctttgaaa ttaccgtatc tagtaggcat attgacagtt gaaactcgtt
    22321 ctatcagctg atcatatact tttctatatt cttgtaattc tttgatggta attagtggaa
    22381 tgttgtgttt cttcgagaac tgaattaaat catctgttct catcattttg ccatcatgat
    22441 tcattatttc acaacatagg ccacactctt ttagtccagc taattttaat aaatcaacag
    22501 ttgcttctgt gtgtccattt ctttctagaa caccaccttt ttttgcaatt aaaggaaaca
    22561 tgtgtcctgg cctgcgaaaa tcagagggtg ttatatcttc agctacacac atacgtgcgg
    22621 tcagtcctct ttcctcggca gaaatacctg tggtcgtttc tttataatca attgaaactg
    22681 taaaagcagt cttatgatta tctgtattgt tttcaaccat aggtgaaagc attaattgat
    22741 tagctaaact ttcgctcata ggcatacaaa ttaatccttt ggcataagta gccataaaat
    22801 taacattttc tgttgtagct gcttgtgcag aacaaattaa gtctccttca ttttctctat
    22861 ccttgtcgtc tataacaaga acaagtcgtc ccttctgcaa tgcttctaat gtttcttgta
    22921 tttttcgata ttccattgac tgattatcct ttctgctaaa atccattttg atataatagt
    22981 tccttagata tttctgattt tggagagtta tccatcagtt tttgcacata tttacctaag
    23041 atatcatttt caagatttac tgtactcccg acttgtttac ttttaagaat ggtttgtttc
    23101 aaggtatgag ggataacaga tactgaaaag tttactttgg agactttagc gacagtcaga
    23161 ctaatgccgt caattgtaat agatcctttt tcaactatta aatctaaaat ttctttttgt
    23221 gtgttgattt gataccatac agcattatca tcttttttta ttgacgagat ttttcctgta
    23281 ccatcaatgt gtcctgtaac gacgtgaccc ccaagtcgac cgttgataga taaggctctt
    23341 tctagattca cctcacttcc atgttttaat agagtaagag ctgttcgact ccatgtttca
    23401 ttcattacat caactgtaaa ggattgatga ttgaaatgag taactgtaag acagatacca
    23461 tttactgcta tactatcgcc taaatggata tccgttaata tttttgaggc tttaattgat
    23521 agtttacaat tacgagagtc tttctgtatt ctttcaactt ttccgatttc ttcaattatt
    23581 cctgtgaaca tggataaatc acttcacttt ctatgagata gtcatttcct atttgagaaa
    23641 aagcataagg tttcaatcta atagcgtcat ttggcaaaga aatgccttca cctccgacag
    23701 gaaacttggc actgcctcca aaaatttttg gtgcaatata tattttcagc tcatcaacaa
    23761 tttgttgttc caaagcactc caattcatta gactgccccc ttctagaact aggctatcaa
    23821 tctgcatgtt tcctagatgt tgcattaaac tcgataagtc tatatgattg ccttttttct
    23881 ttatggaaag tatttcacag ccatgatttt gatatagctt cattttattt ttgtcttcag
    23941 aggaagtggc aatgtaagtt ttaatatcat ttgctgtttt tacgatttta gaggtaagag
    24001 gagttcgtaa atgtgtatcg catatgatac ggataggatt tttcccttcc tccaatctac
    24061 atgtcagcaa aggatcgtct tgaataacag tattgactcc caccataatt gcactaacat
    24121 ggtgtcgtaa ctgatgcaca tgctttcttg cttcttcttc agtaatccat ttggattgat
    24181 ttgttttagt ggctattttt ccatccattg acattgcata tttcataaaa acatagggta
    24241 catgctgggt aatatacttt ctaaaacttt ttattaagtt aagacactca ttttctaaaa
    24301 ttccaacagt aacttgaaga ttattttcct caagtatctt tactcctttt ccagatacaa
    24361 taggattaca gtctaggctt ccaatgacta ctcttgtaat accactatcg attatagcat
    24421 ctatacaggg aggtgttttc ccgaagtgac aacagggttc aagtgttaca taaagcgtcg
    24481 ctccgacagg ggattctcta cagtttttaa gagcatttct ctcagcatgt gggccaccaa
    24541 aaaactcatg ataaccttgt ccgatgatgt gattatcttt tacaataact gcgccgacca
    24601 taggattggg attgacgtaa ccagcccctt tttgtgccag ttttattgct aatttcatat
    24661 attttgaatc gctcatctca ctacctccaa aaaaaatata ccttgaatag gggactactc
    24721 aaggcataca aaagaaaact tatgcgatta acaaaaatac tctgaaatga caagtaatca
    24781 tttcagagca cgcaaaaagc acaaatatac ttttatcttc tttcatccag actatactgt
    24841 cggctttgga atttcaccaa atcatgcctt tcggctcgtg ggctatacca ccggtaggga
    24901 attacaccct gccctgaaga tagttattca attacagatg attatagtac ttaattttga
    24961 atatgtcaac agataaatac cgattgtttt tgatatactg tatttgtgat aatcgattct
    25021 cgctcctcgg ataaagaaaa tatgatatac tagataaacg aaataagaga gaaggaatac
    25081 tatgtacgca tatttaaaag gaatcattac caaaattact gccaaataca ttgttcttga
    25141 aaccaatggt attggttata tcctgcatgt ggccaatcct tatgcctatt caggtcaggt
    25201 taatcaggag gatcagattt atgtgcatca ggttgtgcgt gaggacgccc atttgcttta
    25261 tggatttcgc tcagaggatg agaaaaagct ctttcttagt ctgatttcgg tctctgggat
    25321 tggtcctgta tcagctcttg ctattatcgc tgctgatgac aatgctggct tggttcaagc
    25381 cattgaaacc aagaacatca cctacttgac caagttccct aaaattggca agaaaacagc
    25441 ccagcagatg gtgctggact tggaaggcaa ggtagtagtt gcaggagatg accttcctgc
    25501 caaggtcgca gtgcaagcaa gtgctgaaaa ccaagaattg gaagaagcta tggaagccat
    25561 gttggctctg ggctacaagg caacagagct caagaaaatc aagaaattct ttgaaggaac
    25621 gacagataca gctgagaact atatcaagtc ggcccttaaa atgttggtca aataggagca
    25681 gagaatgaca aaacgttgtt cgtgggtcaa gatgacaaac ccgctctaca tcgcctatca
    25741 tgatgaggag tggggtcagc ccctccatga tgaccaagca ttgtttgagt tgttgtgtat
    25801 ggaaacctat caggcaggcc tgtcttggga aacggtactc aacaaacgcc aagctttccg
    25861 agaagccttt catagctatc aaattcactc agtcgcagag atgactgaga ctgaattgga
    25921 agccatgctg gagaatccag ctatcattcg aaatagagcc aagctttttg ctacacgcgc
    25981 taacgcccaa gtccttctac agttacaggc agagtacggc tcttttgatg cctatctttg
    26041 gtcttttgtt gaggggaaaa ttgtcgttaa cgatgttcct gattatcgcc aagcgccagc
    26101 taaaacaccc ttatctgaga aattagccaa agatctcaaa aaacgaggct tcaagttcac
    26161 aggcccagtc gccgtattgt cttttctaca ggctgcaggg ctagttgatg accacgagaa
    26221 tgattgtgag tggaaaggtc ttaaatgatg tctaacaaaa ataagggaat tctgattttt
    26281 gcgattctct atacagtcct ctttgtgttt gatggcgtta aattgctggc ttctttaatg
    26341 ccatctgtca ttgcaaatta tcttgtttat gtagttttag ctttatatgg ctccttcttg
    26401 ttcaaggata gattgatcca acaatggaag gagattagaa agactaaaag aaaattcttc
    26461 tttggcgtct taacaggatg gctctttctc attctgatga ctgttgtctt tgaatttgta
    26521 tcagagatgt tgaagcagtt tgtgagacta gatggacaag gtctaaatca gtctaatatt
    26581 caaagtacct ttcaagaaca accactactg atagctgttt ttgcttgtgt cattggacct
    26641 ctggtagaag aattattttt ccgtcaggtc ttattgcatt acttgcagga acggttgcca
    26701 ggtttactaa gcattattct ggtaggactt gtttttgctc tgactcatat gcacagtttg
    26761 gccctatcag agtggattgg tgcagttggt tacttaggtg gaggccttgc cttttctatt
    26821 atttatgtga aagaaaaaga gaatatctac tatcccctac ttgttcacat gttaagcaac
    26881 agcctctcct taatcatttt agctatcagt atagtaaaat gaaatgagaa caggacaaat
    26941 cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat atactgtaat
    27001 atgtgatgaa aatgccagta atgataccga gaaaaaagct gagaaacttt tcccagcttt
    27061 atttgttata gtcaaagaga atgacttgtt cctgtgcatc tacatgagca tggaccccaa
    27121 agggtacaat tgctcttgga gttgcgtggc cgacattcag attatagaca atcgggatat
    27181 tgctgtcaat gatatccaat agtgcctctt tatagtcgtc atggaaagtt tcatccatag
    27241 gttttccgac caagagtcca ttgatgaccg cgaatatgcc agtgtccttt aaagttagca
    27301 acatcttttt gaagtcttct ggcttaggct tttcttcgct tgtttcgagc aagaggattt
    27361 tcccttccca gtctgacaag tcagggaaaa gtttgtattt ttggcagagt tccgtgctat
    27421 ctgcgtatcg agagttgtca aagatatcgt agagggattc gaggcaacca ccgaggattt
    27481 tcccctcgaa ctgggcactt ccttgcaaca agtcaaaacc tgtatttgta tgactgacac
    27541 gaggtgttcc cagggccgtg ggactaaaat cagttcgttc ctcataccaa acgtcactag
    27601 ggcggatttc tgaaattctt cccgtctcaa tcaattcttt aaagtagtga aggctatagg
    27661 ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca taaaaagtct
    27721 tgattcctaa tttatgcaac atgaggtggt tcatggttgt atccgagaag ccaagaaaaa
    27781 ttttttgttt gataaccttt tggagttggt cattttcaaa aagataaggt agcaagcgat
    27841 aggtatcgtc tccaccgatg gcacatagga tcatgtcgat gctatcatca gaaaaggcat
    27901 gaatcaaatc ctctgcacga gcttcaggat ggtccttgat aaagtctaat ccttttaacg
    27961 aatggggcaa aaagatggga ttgagtccca gatccttgag acgttggata cccaagtcca
    28021 cttcgtgttt gacaaaatcc tctccgataa tgccactaga taaactaaca ataccaatag
    28081 tagaaaccat atctcatcct cctagaaata gattgagcct attttatcac aaaatataag
    28141 agaaagctat gtgggatttc agaagaatac ttttaatact cttcgaaaat ctcttcaaac
    28201 cacgtcagtt ttatttgcaa cctcaaagct gtgctttgag caacctgcag ctagcttcct
    28261 agtttgctct ttgattttca ttgagtataa ctcaaaaaag taagtgaaaa agcaaccgca
    28321 cctgcagttg cttttatcgt tttcttattc ttgatagcgt gttggtcctg cacttgcact
    28381 acggatattc aagcgtttct tgagatagaa gcgaagggct aggagggcta ctccaataat
    28441 agctagtggc aatggatcca gtactgggtt aaggctagct ggtaggaagc ttgttgcaaa
    28501 gaagacaagc aaccaaagga acatagaagt taggataact agtacagatt tccagaaagg
    28561 tggacgttga ctgcgatcca tatctggtcc atagtattgg taaacaaagt agtacatcaa
    28621 gtagaaggca aatccaccaa ccagtccaac taatagaaga gtaatcaatc catagccgaa
    28681 agcttgatct gccgcaaaga aggttgtgag ggcgctgaca agggcaaaga ggctagtgat
    28741 gaaaagagct gagtccataa tcatcagttt tggatcatca ttttcttttg gatgctcttt
    28801 ttcgtactgc tctttgacag tgaagctatg agcccaatga gttggtgcgc cgtataggga
    28861 acgggcagtt gtacctttag attgctcctc aaggatttgg ggaatgactt cctcaaaaat
    28921 agtctggatt tcagcgtctg ttttcccatc tttgatgaat tgttgggtag caatgtggac
    28981 aaactcttgg tttttcttag ttaatttttg tagatcaatt tgagacatag gaactcctct
    29041 tagaaccatt ttttatggat gagatagaga gtgagcgaga cactcatagc aaaggcgata
    29101 aagacgatta accagaaggc atttggctct ccgtttaggg gaatttcatt atccttaaag
    29161 ttcatcccgt aggcagaaaa gaccatggtt gggatggaca tgacgatggt cacaagggcc
    29221 aaggttttca tgatgttgtt ctggttgtta gaaatgatag aggcaaaggt ctctgtcata
    29281 gaatgcaaga cgtttccata aatatctgcc atctcgatgg cctgttgggt ttcaatcagg
    29341 gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga tattgctggt tgaactggtc
    29401 aatttcttaa tcacgcgctc atttgttttg agggaggcct tgaaatagac gatagttttt
    29461 tccaattcca tgagctcaat caattcttca ttacgagttg attgatgcag ttgactttcg
    29521 atttgttcac tcttgcggtc gattgaacga agggctgtta ggtaaagctc tgcattgcga
    29581 taaagaattt gaaagataaa acgtgaacgc atgaaggtat agaaattacg caatcgacgg
    29641 ttgataaaga catcaaggac aggtagtggt tccaaacacg tagtgataat ggtttcctca
    29701 gtgatgataa taccaagcgg gatggttacg tagtaggtgc ggttatttct ttcctccgtg
    29761 accggcacgt ctacgataat cagggtatac tcgtcttcaa tggtaatacg agacatttct
    29821 tccgcatcga gcggtgctcg aaggtcggca atatcaatat cgaaggtgtt ggcgatttcg
    29881 agtgattcat tttgagtcgg attgacgaga ttgatccaag tacccggttc aagcgtatcg
    29941 atctctttaa attcagttgt tgtagagaga aaaacttgtt tcatatccct tatcctttct
    30001 catttttcag attttttcac actgtactat tatactacaa aatcggcctt ttgggtaata
    30061 gaatctcact ttttttggta taatggtaag caataatgga ctagaaagaa caaagatgca
    30121 agataaaatt gtcattcatg gggcgcgtgc ccataattta aaaaatattg atgtggagat
    30181 tccgcgagac aagttggttg tcgtgactgg cttgtcaggt tcagggaagt ccagtctggc
    30241 ttttgatact ctctatgcgg agggacaacg tcgctatgta gagagtttgt ctgcctacgc
    30301 tcgtcagttc ttgggaaata tggaaaaacc agatgtagat gccattgatg gtctcagccc
    30361 agctatttcc atcgaccaga aaacgactag taaaaatcct cgctcgacgg tgggaacgac
    30421 gactgaaatc aatgactatc tgcgtctcct ctacgcacgt gtggggacgc cttactgtat
    30481 caacggacat ggagctatca atgcttcttc tgtggagcaa atcgttgata aggttttgga
    30541 gttacctgaa cgccagcgct tgcagatctt ggctcctgtc atccgcaaga aaaaaggcca
    30601 acataagagt gttatcgaga aggttcagaa agacgggtat gttcgtgttc gtgtggatgg
    30661 ggaagtctat gatgtgaccg aagtgccaga gttgtctaag agcaagcaac acaatatcga
    30721 tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt agccgtctct ttgattccat
    30781 tgaagctgcc cttcgtatcg cagaaggtta tgtgattatc gacaccatgg acgactctga
    30841 gttgttgttc tctgagcatt atgcctgtcc agtttgtgga tttactgtcc cagagttaga
    30901 acctcgtctc ttctctttca atgctccctt tggctcttgt agtgagtgtg acggcttggg
    30961 gattaagctg gaggtggata ctgatttggt agtgccagat gccagcaaaa ccttacatga
    31021 gggagcgctg gcaccttgga atcctatctc atccaactac tatccaaaca tgttagagca
    31081 ggccatgaaa gtctttggag tggctatgga taagcctttt gaggacctgt cagaagaaga
    31141 taagaacttg attctctacg gctcagatgg taaggaattc catttccatt atgaaaatga
    31201 atttggtggt gtgcgcgata tcgacattcc gtttgaggga gttatcaata atatcaaacg
    31261 tcgttaccat gaaaccaata gcgattacac tcgcactcag atgcgtctct acatgaatga
    31321 gctgacctgc ggaacctgtc aaggctatcg tctcaatgac caggccttgt ctgtccgtgt
    31381 gggcggccag caagggccac atattggaga aatctcagac ctgtctatcg ctgaccactt
    31441 ggacttggtg agccagttaa ctttgtctga aaatgaagcc atcattgctc gtcccattct
    31501 caaggaaatc aaggatcgtt tgaccttcct taataatgtg ggtcttaact atctaaccct
    31561 gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt tggcaaccca
    31621 gattggttcc aacctatcag gtgtcctcta tatcctagac gagccgtcaa tcggtcttca
    31681 ccagagggac aatgaccgcc tgattgccag tctgaaaaag atgcgtgact tgggcaatac
    31741 tcttatcgtg gtggaacacg acgaagatac catgcgtgaa gctgattatc tgattgacgt
    31801 tggtcctggt gccggtgttt ttggtggtga gattgttgca gcaggtacac ctaagcaagt
    31861 ggctcgcaac agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg tcatcccagt
    31921 accagaagag cgccgtgtcg gaaatggtcg ttttattgaa gtgacaggag cgcgtgagaa
    31981 caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag tgacaggtgt
    32041 gtcgggttca gggaaatcga ctctaatcaa cagcattctc aaaaaagcca ttgcccagaa
    32101 gctcaaccgt aattcagaca aacctggtaa gtttaagact attacaggga ttgagcatgt
    32161 cgaccgtttg attgatattg accagagccc tatcggacgg acgccgaggt ctaacccagc
    32221 tacctataca ggagtttttg acgatatacg tgaccttttt gctcagacaa atgaagccaa
    32281 gattcgaggc tacaagaagg ggcgcttcag tttcaacgtc aagggaggtc gctgtgaagc
    32341 ttgctcaggt gacgggatta tcaagattga gatgcacttc ttgccagatg tttatgtggc
    32401 ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc ctagaagttc actacaagga
    32461 aaagaatatt tcgcaggtct tggatatgac ggtcaacgat gcggtagaat ttttccaaca
    32521 cattccgaaa attcaacgca aactccagac catcaaggat gtaggtctag gctatgtgac
    32581 cttgggacag ccagctacca ccctttctgg gggagaagcc caacgtatga agttagctag
    32641 cgaactccac aaacgctcga caggaaaatc tttctacatt ctggatgagc cgacgacagg
    32701 gcttcataca gaggacattg ctcgcctgct caaggtttta gctcgctttg tagacgccgg
    32761 caatacagtc ctcgtcatcg agcacaatct ggatgttatc aagacggcag accatatcat
    32821 tgacttggga cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag gaactccaga
    32881 agaagtagct gccaacgaag ccagctatac aggacactat ttgaaaggaa agttacatca
    32941 tgaataaacg cgtacaagca tttctagcta aaatgcaaga aaaagaacta gatggtatca
    33001 tcatcaacaa tcttaaaaac gtctattatt tgactggttt ttggggctca aacggaacag
    33061 tctttatcag ccgtgaccgt caggtcttag tgacagactc tcgctatatc atcgcagcta
    33121 agcaagaaac cagtggtttt gagattgtgg ctgatcgtga tgaattggct gtcattgcag
    33181 gaattgttaa ggacatgaac ttgactcgta tcggttttga agatgagatt tcagtgtctt
    33241 attaccaccg tatgcaggca gcttttgcag gtttgaactt gcttccacaa actcagtttg
    33301 tggaaggtct tcgtatgatt aaggatgagg cagagattgc agcgattcgc aaggcttgtt
    33361 ctatctcaga ccaagctttc cgcgatgcgc ttgactttat caaaccagga aaaactgaaa
    33421 ttgagattgc caacttcctt gatttccgca tgcgtgagtt gggagcatct ggcttatctt
    33481 ttgatacgat cctagctagc ggtatcaatt cttctaaacc ccatgcccat ccaatgcaca
    33541 aaccagtgga gttgggagaa gccattacca tggacttcgg ctgtctctat gaccactatg
    33601 tcagtgatat gacccggact atctatctag ggcatgttag cgatgagcag gcagagattt
    33661 acaatacggt tctaaaagct aaccaagcct tgattgacca agctaaggca ggcttaggtt
    33721 tccgtgactt tgacaaaatc cctcgtgata ttatcattga ggcaggttat ggtgactact
    33781 ttactcacgg cattggccac ggtattggtc tggatatcca tgaggaaccc tactttagtc
    33841 agacttctac agaaactatt aagacaggta tggccttgac cgatgaacca ggtatctata
    33901 tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct gattacagag acaggttgtg
    33961 aattattgac cctagctcca aaagagttga tagtcattta gttgtttgtc aagaaaaaag
    34021 tacgaaaatg acgaaaaaag tcaaaaaaaa ttaaaaatag gtcgcaagtc ggatgttttt
    34081 tatggtataa tagactaaac tgatagtaac atgtagcgaa aggggtaggt acatgattaa
    34141 aatttataca gtctcaagtt gtactagttg taaaaaagca aaaacctggc tcaatgccca
    34201 ccagttaagt tataaagaac aaaaccttgg taaagaagga attacgagag aagaattact
    34261 ggatattcta accaaaacag ataacggaat agccagcatc gtttcgtcta aaaatcgcta
    34321 tgccaaagcc cttggagtgg atattgaaga tttgagtgtc aatgaagttc tcaatctgat
    34381 tatggaaaca ccgagaattt taaagagccc aatccttgta gatgaaaaac gcctgcaagt
    34441 tggctacaag gaagacgata ttcgtgcctt cctaccacgc tctgtccgta atgtagaaaa
    34501 tgcagaagca cgtttgcgtg cagctctata aacatcaagg ctgggagcac ttcccagtct
    34561 tattctattt taatttcaaa aagaaagaag aaagaaatga aaaaaatagt tcttgttagt
    34621 ctagctttcc tttttgtcct ggttggttgc ggacagaaaa aagaaactgg accagctaca
    34681 aaaacagaaa aagatacgct tcagtcggca ttgccagtta ttgaaaatgc tgagaagaat
    34741 acagttgtaa ctaagacttt ggtcttgccc aagtcagatg atggtagcca gcagacacaa
    34801 acaattactt acaaagacaa gacttttttg agtctagcta tccaacaaaa acgtccagtc
    34861 tctgatgagt tgaagactta tattgaccaa catggagtgg aggaaactca aaaagctctt
    34921 cttgaagcgg aggagaagga taagtctatc attgaagctc gtaaattggc aggtttcaaa
    34981 cttgaaacaa aactattgag cgcaacggaa cttcaaacaa cgactagttt tgattttcaa
    35041 gttctggatg tcaagaaggc ttcccagttg gaacatctga agaatattgg tttggaaaat
    35101 cttttgaaaa atgaaccaag caaatatatt tcagatagat tggcaaatgg cgcgacagaa
    35161 caatagaaaa tccaaataaa tagactgcct gaattgtaga aggtaaggaa ttatgctata
    35221 atggtactat tctaaggaaa gagggtgtta gaatgggatt tactgaagaa acagtacgtt
    35281 ttaaattgga cgattccaat aaaaaagaaa ttagcgaaac tttgacagat gtttatgctt
    35341 cgttgaacga taagggttac aacccaatta accaaatcgt aggttacgta ttgagtggag
    35401 accctgccta cgttcctcgt tataataatg cacgaaatca aatccgtaag tatgagcgtg
    35461 atgaaatcgt tgaggaattg gttcgctact acctcaaagg acaaggagtc gatctataac
    35521 ctatgagaat tatgggattg gacgtcggtt caaaaacggt aggggtggcg attagcgatc
    35581 cgcttggttt tacagctcaa gggcttgaaa tcatccagat aaatgaagaa caaggccaat
    35641 ttggttttga ccgcgttaag gaattggttg atacttacaa ggtggaacga tttgtagtgg
    35701 gcttgcctaa aaacatgaac aatacaagtg gaccgcgcgt agaagctagt caagcctacg
    35761 gagcaaagct agaagagttt tttggtttac cagtagacta tcaggatgaa cgcttgacaa
    35821 cagtggctgc tgagcgcatg ttgattgaac aagcagatat cagtcgcaat aagcgcaaga
    35881 aagtcattga taagttagca gctcagctga ttttacaaaa ttatttagat agaaaatttt
    35941 aatataaagg agaggctatg tcacacgatc ataaccacga ccacgaagaa cgtgaactaa
    36001 tcacactagt agatgagcaa ggaaatgaaa ccttgtttga aatccttttg acaattgatg
    36061 gaaaagaaga atttggtaaa aactatgttc ttctagtacc agttaacgca gaagaagacg
    36121 aagacggaca agttgaaatc caagcttact cattcattga aaatgaagat ggaacagaag
    36181 gcgaattgca accaatccca gaagactcag aagacgaatg gaacatgatt gaagaagtct
    36241 tcaacagctt tatggaggag tgatacagtc tgggagactg tatcagccca actccagaaa
    36301 tggggaagag ttggaacgtc cagtgaacat ttttacccca actcttagaa attgagaaga
    36361 gttggaacat ctagtggatg tttttagccc atattcacat tcatagtagc tagttattag
    36421 ctaaccaggt aagaggtcgg gacgaaaatc ctggccttgt ttttattagt aaccatactt
    36481 taatgcgata attgattgga cttttgtcaa ggagatgtgt atgtttgaag tagaagaatg
    36541 gctccatagt cggattggtt tgaattttcg atcaggtttg ggccgaatgc agcaagcggt
    36601 ggatttgtta ggaaatcctg agcagtctta ccctattatc cacgtaacag ggactaatgg
    36661 gaaaggatct accattgctt ttatgaggga attatttatg gggcatggca aaaaagttgc
    36721 gacctttacc tcccctcata tcgtctctat caatgaccga atctgcatta atgggcaacc
    36781 tatagcagac gcagacttta tccgtttgac tgatcaggtc aaggagatgg agaaaacgct
    36841 tctgcaaact cctgcccagt tgtccttttt tgaattgctg accttagttg cttttcttta
    36901 ttttagggag caagaggtgg atttggtttt attagaagtg ggaattggtg gcttacttga
    36961 cacgaccaat gtggtaactg gagagcttgc tgtcatcacc tccattgggc ttgaccatca
    37021 agaaaccttg ggtgatagtc tagaagcaat tgcagagcag aaagctggta ttttcaaggc
    37081 tggtaaaaag gcagtgattg cgaaattgcc tccagaagct agacttgtct gtcagaaaaa
    37141 agccgaatct ttagctgttg acctttatca ggcaggtcaa gatttttcag tgctgaatgg
    37201 tgatttttca agctctttac taaatctttc acagctgaac ataggcttag aaggagccta
    37261 tcagcaggag aatgcggcct tggcgttgca aacttttctt ctttttatgg gagaaagaaa
    37321 ggaagctatt gatgaacagg ctgtaagaaa ggccttggaa cagacccatt gggctggtcg
    37381 cttggagcgt attcgcccac agatttactt ggatggtgct cataacctcc ctgccttgac
    37441 tcgcttggtt gagtttatca aagaaaaaga gcaggaaggc tatcgacctc aaatcctctt
    37501 tggatccttg aaacgtaagg attatcaagg gatgttgggt tatctgactg aaaatttacc
    37561 tcaagtggaa ctcaaaatga ccggctttga ctatcaaggg tctttggacg agacagatgt
    37621 aacaggttac catgtaattc cctcttaccg agaatttatc agcagtttcg aagaaagggc
    37681 agacgctcaa gacttgctgt tcgttacagg gtctctctat tttatctcag aagtacgggg
    37741 ctacctgctg gaccgtgagc agataaattg accccctttt ttgaacttgt tatactaggg
    37801 gaattgctgg ttagagaaaa tttctctaaa ttggtagcag aaaggaaatt catcatgaaa
    37861 ttaaaaagat tcacactttc tcttgcttct ctagcaagtt ttagtctctt agtagcttgt
    37921 tcacaaagag ctcaacaggt tcaacagcct gttgctcagc aggtccaaca gcctgctcaa
    37981 cagaatacca atactgcaaa tgcaggaggt aaccaaaatc aagcggctcc agtacaaaac
    38041 caacctgttg ctcaaccgac cgatattgat gggacttata ctggtcagga tgacggagac
    38101 cgtatcactt tagtggtaac tggaacgact ggtacatgga ctgagctcga atctgacggg
    38161 gatcagaaag tcaaacaggt tacatttgat tcagcaaatc aacgcatgat tattggcgat
    38221 gatgtcaaaa tttacactgt aaacggtaat caaatcgtcg tagatgatat ggatagagac
    38281 ccatcggacc aaatcgtttt aactaaataa gactatgcaa gtagaaaggg ctggattttt
    38341 cagccttttt acttttacct agaaaataat cataaatact tgccttctac cgaatacctg
    38401 cgttatacta gtatcaatta cttgttttta gcattcaaac tatcaaggag gggatatgaa
    38461 atataggaaa tttcaattat tgatgtccaa gtatggcttt agtctttcga ttatgctact
    38521 tgaactttgt cttgtttttg gtctctttct ttatttagga cgcatggctc ccattttatg
    38581 gattactgtc ctcattctac tgagtatcat cacaatcatt tcgatagtca accgtaatac
    38641 gactcctgag aataaggtaa cctggttgtt agtagccttt gtgccagtat ttggtccctt
    38701 gctctatctg atgtttggtg aaaggcgatt gtccaaaaaa gaaatcaaac aactgaagaa
    38761 gctaggctct atgcatttcc aagaagcaaa tagccagcta ctaaaagaga aattaaaaga
    38821 aagtgacaag gcagcttatg gagtcatcaa gtccttattg agtatggata ccaatgctga
    38881 catctatgat caaactgcct ctacattttt tcctaacgga gaagctatgt ggaaaaagat
    38941 ggtagaagat cttaaaaagg ctgagaaatt tattttcttg gaatattaca ttatagaaga
    39001 aggtttgatg tggaatcgca tactagatat actagagcaa aaggtagctc agggtgtaga
    39061 ggttaagatg ctctatgatg atatcggctg tatggctact ttaacaggag attatgcaca
    39121 tcgacttcgt cagctgggca tcgaggccca taaattcaat aaagttattc ctcgtttgac
    39181 agtggcttat aataacagag atcatagaaa aatattgatt gttgatggtc agatagccta
    39241 tactggtggg gtcaatctgg cagatgagta cattaaccac gtcgagagat ttggttattg
    39301 gaaggatagt ggaattcgct tagacggact agcagtaaaa gccctgacac gcttattttt
    39361 gaccacttgg tacattaatc gaggagaaat tagtgatttt gatcaatatc atttagaaaa
    39421 tcattctatc ccgagtgacg gtttaaccat tccatacgga agtggaccca agccaatttt
    39481 tcgagcgcag gtagggaaaa aagtttatca gagtttaatc aatcaagcaa cagaatcggt
    39541 ctatattacg acaccttatt tgattataga ttatgattta acagagacaa tcaaaaatgc
    39601 agctatgaga ggggtcgatg ttcgaattat caccccttac ataccagata agaagttcat
    39661 tcagttagtc acgagaggag cttatcccga ccttctttct gctggtgttc ggatttatga
    39721 gtatagtcca ggttttattc atagtaagca gatgttggta gacgaagatt ttgcggtggt
    39781 ggggacaatc aatctcgact accggagctt ggtacaccat tatgaaaatg cagtcttact
    39841 ctataaaact ccttctataa gggaaatcgc ccgagatttt cgaaatatat ttgcagattc
    39901 tcaggaagtc tatcctcatt ctatcaaaac gagctggtat caaaagcttg taaaagaaat
    39961 cgcccagcta ttcgccccta tcttataaga aatctagaac ttaggataca aaccagacct
    40021 atttttcttg tcttttacta atagaaagaa agaggagggg aattcatgct aactaaggaa
    40081 gaagtgaatg atatgattga gttcaaacag acacatttat accgctttcg ggaaattgaa
    40141 acctttgtga aactatgcaa aaaatcactc aaacagccat cgcaagctga caatcctagg
    40201 actttttgat atactaagac agataaattg aacaggagaa acgatatgag tgtgtatggt
    40261 agagtagaag aagttcataa ggagaatcgt gaacctctag aataccaaat cgaacaagaa
    40321 tcgcatcatc gtgaatcaag tcgtcttcct ttggtgaaaa ttttactatg gagtacgctt
    40381 gtaacgggga taactctagg agtaccgcta ttactcgact taatgagtgc acaagaagtg
    40441 caggattttt atgcaggttg ggggcttcat cagacaggga agatttacag cgactattat
    40501 ggaagtcaag gtttgcttta ttatttgctg acttacgtga gtcagggcgg atttttcttt
    40561 gccatctttg agtggttagc cttggtagca ggaggatttt tcctttttag atcagcggac
    40621 accttgacag agcaaggaga ccaagctgga catctggtga ctatttttta catgctagtt
    40681 acaggtcttg cttttggtgg aggctatgcg actcttttag cgcttccttt cttattcgca
    40741 gcctttagtt tagttgcggc ttacctaagc aatccaagcc atgataaggg atttgtacgg
    40801 attgggctag ctttggcagg cggatttttc tttgctccct tatcatcgct cctgtttatt
    40861 gctgtagtga gtttaggctt gttggtcttt aaccttgggc atagacgctt tgcgcatggg
    40921 ttttatcagt ttcttgcagt ggctttaggt ttttcacttg tcttttatcc aactgcctac
    40981 tatagtgctg caacaggaag ttttggggat gcgattagtg gtattcgtta tcctattgac
    41041 agtattcgct ttgattttac ttctaaaatt ttagagaata tgttttttta tggcttgttg
    41101 tcccttggtt tgggatttgt ggtttgtatc tttttaggtc tctttcaatc caaacccttt
    41161 aaactatacg tcatttcggt tcctgcaagt cttgtggtaa ttttaggttt gattttactt
    41221 ttcttttcac aagagcctct gcacgcttct tatttgatgg tcgtcttccc tgttttccta
    41281 cttttattgg taaccaatat taagagtcaa cagagggggc gtagtgctag aagaagccga
    41341 agagaaacgc cagttagcct atggagtcgt ttcttcaaag gaaatctata tctgctagtt
    41401 tttgggtttg tctatctttt gtctgttcct tttttgatga agtttgtcct ttatccagta
    41461 ccttatcaag aacgtaatcg tcttgctgat ttggtaaaag aggagacaaa tacggaagat
    41521 gctatctatg catgggatga tactgcgact ctttatcgta agagtgagcg cttgtcgcca
    41581 tcggcgattt tgtccccgtt gcactataca gcaactgagg aaaatcgtaa taagttactt
    41641 aatgacttga aagaaaaaca acctaaggtg attgtggtaa atgacaaggt ggtagtctgg
    41701 tctgaagtgg aaacactttt aaaagaaaat taccaacaag taaagactga ttactcagag
    41761 tttaaagtct ataaaattaa ataaccaaat caatatcttg tgtattttta aaaattttag
    41821 gatttttaac acaagatatt gatttttctt tttagagtgg tataatactt tttagaaaga
    41881 acattttaga aaagagcatg cgtatgattg cactagaaga aaaaattaca attttgccaa
    41941 ctctcttcgt cgagaaacga gatgggagac gtgttgtatt tgatgtggac aagattgaca
    42001 aggctctcca caaggcggct gacaaggtta tggatgtgac acccctggtt gaaaaacgcc
    42061 tcaatgatct gactgagcga attattacag aaattcatag tcgctttcca cagggaatta
    42121 agatttacga aattcaaaat atcgtagaac atgaactcct tgaagccaaa gaatatgcgc
    42181 tggctgagga gtatattact tatcggacac agagggattt tgagcgctca aaagcgacgg
    42241 atatcaactt tagtattcat aaacttctca acaaagacca gacagttgtc aatgaaaatg
    42301 ctaataaaga cagtgatgtc tttaacactc agcgtgattt gacagcaggg attgttggga
    42361 aatcaatcgg actgcaaatg cttcctaagc acgtagccaa tgcccaccaa aagggggata
    42421 tccactatca cgatttggac tacagtccct atacccctat gaccaactgc tgtttgattg
    42481 attttaaggg tatgttggaa aatggtttta agattggaaa tgcagaggta gagagtccca
    42541 agtctatcca gactgcgaca gcacagattt ctcaaatcat tgccaacgtt gcttctagcc
    42601 agtacggtgg ctgttcagct gaccgtatcg atgaaatttt ggcgccttat gcagagaaga
    42661 attatcaaaa acatctcaaa gatgcagaag agtgggtatt gcctgaaaaa cgggaagatt
    42721 acgcttggaa gaaaacgcaa aaggacatct acgatgccat gcaatctctt gagtatgaaa
    42781 tcaatactct cttcacttca aatggacaaa caccttttac ttcgttaggt tttggtctgg
    42841 gaaccagtcg ttttgaacga gaaattcaaa aagctatttt aaacattcgc atcaagggtc
    42901 ttggttcaga acaccgtacg gctatctttc ctaaacttat tttcactctg aaaagaggcc
    42961 ttaatttaga ggaaggaact cccaactacg acatcaagca gttggctctt gagtgtgcaa
    43021 ccaaacggat gtatccagat gttttatctt acgataagat tgttgacttg acaggatcct
    43081 tcaaggtgcc tatgggttgc cgttctttcc tccaaggatg gaaggatgaa aatggtgtag
    43141 aagtcaattc aggtcgcatg aatctgggtg ttgtgacggt taatctgcct cgtattgctc
    43201 ttgagtctga aggtgatatg aataagttct gggaaatctt caacgagcga atgaatatcg
    43261 cagaagatgc tcttgtttac cgtgtcgaac gcactaaaga ggcgacacca gcaaatgctc
    43321 ctattcttta tcagtacggt gcttttggcc atcgtctagg taaagaagaa agtgttgacc
    43381 agctctttaa gaatcgtcgt gctaccgttt cgctgggcta tatcggcttg tatgaagtag
    43441 cgacagtttt ctttggtaac agctgggaaa gtaatccaga tgctaaggaa ttcacgctag
    43501 acatcattca cgatatgaaa cgccgtgtag aagagtggtc agaccaatat ggctaccatt
    43561 tctctatcta ctcaacacca tccgaaagtc tgacagaccg tttctgtcga ctagatatag
    43621 acaagtttgg ctctattcct gatatcacag acaaggaata ctacaccaac tctttccact
    43681 acgatgttcg taaaaatcca acaccgtttg aaaaattgga ctttgagaaa gtctatccag
    43741 aagcaggtgc gtcaggtggt ttcatccatt attgtgagta tccagtcctt cagcaaaatc
    43801 caaaggcctt ggaagctgtc tgggattatg cttatgaccg tgtaggctat ctaggcacca
    43861 atactccgat tgaccgttgc tacaagtgtg actttgaagg ggattttgaa ccaactgaga
    43921 gagggtttgc ttgtccaaac tgtggcaata gcgaccctaa aacagtagat gtggtgaagc
    43981 gaacttgtgg ctacctaggt aatcctcaag caagaccgat ggtcaacggc cgtcacaagg
    44041 aaatcgctgc gcgtgtcaaa catatgaatg gttcaacgat taaaatagct gggcatcaag
    44101 taacaaatta gaaagaaatg aaatgggaaa atatcaacta gacgataagg ggcgcgcaca
    44161 agtgacccgt tatcacgaga aacactctaa aggtggagct ggtaagaaag aacgcttgct
    44221 tagcttcaga gaacaatttt taaacaagaa caagaaaaaa taaaagtgag agccagctct
    44281 cgcttttctc atagtgggag gtaaggatgg aattacgcag accaagatta gcggataaga
    44341 aagctgtttt agatatgatg acagagtttg aaaaatttca gtcgcctcac gacggcggtt
    44401 tctgggatac agagaacttt gtgtatgaag actggttaga aagcaatcag gaacaggaaa
    44461 tggggattaa tctgcctgaa ggatgggttc ctgcaattca gttagtggct ttttctgaga
    44521 aaggtcaagc agttggattt cttaatctcc ggttgcgcct cagtaacttt ctactagaag
    44581 aaggtggcca cattggctac tccattcgtc catctgaaag aggcaagggt tatgcaaaag
    44641 agactctccg tcagggcttg caagttgcta aggaaaagaa catcaagaaa gctctggtga
    44701 cctgtagcgt gaataatcct gctagcagag cagtcattct agcaaatggt ggaatatttg
    44761 aggatgctcg caatggagtc gagcgttatt ggatagaggt agcgaatgaa taatccaaaa
    44821 ccacaagaat ggaaaagcga ggaacttagt caaggtcgta tcattgacta caaggccttt
    44881 aactttgtgg acggcgaagg cgtgcgtaac tctctctatg tatcaggctg tatgtttcac
    44941 tgcgagggat gttataatgt tgcaacttgg tcttttaatg ctggcattcc ctatacagca
    45001 gaattagaag agcagatcat ggcagatctt gctcagccct atgttcaagg tttgactttg
    45061 ttgggagggg agccttttct taatactggg attctcttgc cacttgttaa gcggattcgg
    45121 aaggaattgc cagacaagga catctggtcc tggaccggct acacttggga agaaatgatg
    45181 ttggaaactc cagataaact ggaattcttg tcactgattg acattcttgt cgatggaaga
    45241 tatgatcgaa ctaagagaaa tcttatgctc cagtttcgag gttcatctaa ccaacgaatt
    45301 atcgatgtgc aaaaatcgct caaaagtggg caagtagtga tttgggacaa gctcaatgac
    45361 ggaaaagaaa gctatgaaca ggtgaagaga gaatgaagaa aaaatgaaga aaaaagactt
    45421 agtagaccaa ctagtctcag agatcgagac ggggaaagtc aggacactgg gaatatacgg
    45481 tcatggagct tcaggtaaat caacctttgc acaggaattg taccaagctt tagattctac
    45541 tacagtaaat ttgctagaaa cagatcccta tatcacctcc ggacgctatt tggtagtacc
    45601 caaggacgcg ccgaatcaaa aggtgacagc cagtctgcca gtggcgcatg aactggagag
    45661 tttgcagaga gatatccttg ccttgcaggc gggtatggat gtcttgacaa ttgaagaacc
    45721 ttggaaggct agtgaggtct tgtctggagc caaaccaatt ttgattgtcg aagggatgtc
    45781 tgttggtttt ctacccaagg aactctttga aaaaaccatc tgtttctaca cggatgagga
    45841 gaccgaatta aagcgacgcc ttgctagaga tacgactgtg agaaatcgct atgcatcctt
    45901 tatattggct agccatcaga tgagacggga gcagtatctg cgatactata aagaaactga
    45961 gtctaaggcg gatatcttag tggaccaatc agaagataaa tttgatgtca agaggactta
    46021 acttatgtat agaagaaata tagaagaaaa tccctaattt tagaaagaaa aagtaggaaa
    46081 acagtttcat cccaaaaaaa cgaaaaaacc taacaaatcc cttgcaatcg caggggattt
    46141 gtgttattct attatggtgc tgtaaattac agccttagtt ttgatgcaag aggttgcgac
    46201 acgctcggtt gcattgccac gcaacaccgc gtcggttttc ttgtggagct agcctattat
    46261 cttaaataga cgaaaaggag aaaaagatgg caaacaaaaa aatccgtatc cgtttgaaag
    46321 cttacgaaca ccgtacgctt gacacagcgg ctgcaaaaat cgtagaatca gctactcgta
    46381 caggtgcaca agttgcgggt ccaataccac ttccaactga acgtagcctc tacacaatca
    46441 ttcgtgcgac tcacaaatac aaagactctc gcgaacaatt tgaaatgcgt acacacaaac
    46501 gtttgatcga tatcgttaac ccaactcaaa aaacagttga tgccttgatg aaattggatc
    46561 ttccaagtgg tgtaaacgta gaaatcaaac tttaatctaa aatataaaag agcagaggct
    46621 ggtgtttcaa tctaattgaa cacgggctaa actcggtgtg aaaaagataa acttcctcgc
    46681 gtctgctaga cactgtgtca gtttcctatt ttcactttga gtttgacgcc ctttgtatct
    46741 tagacttgag cataaaaaac gctcgttaaa aactttttga ataaaaaata tagaaaagga
    46801 actattttct catgacaaaa ggaatcttag ggaaaaaagt gggaatgact caaatcttca
    46861 ctgaagctgg cgaattgatc cctgtaacag ttattgaagc aactccaaac gttgttcttc
    46921 aagttaaaac tgttgaaaca gacggataca acgctatcca agttggtttc gatgacaaac
    46981 gcgaagtatt gagcaacaaa cctgctaaag gacatgtagc gaaagctaac acggctccta
    47041 agcgcttcat tcgtgaattc aaaaacgttg aaggcttgga agttggtgct gaaattacag
    47101 ttgaaacatt cgcagctgga gacgttgttg acgtaactgg tacttctaaa ggtaaaggtt
    47161 tccaaggtgt tatcaaacgc cacggacaat cacgtggacc aatggctcac ggttctcgtt
    47221 accaccgtcg tccaggttct atgggacctg ttgcacctaa ccgcgtattc aaaggtaaaa
    47281 accttgcagg gcgtatgggt ggcgaccgcg taacaattca aaaccttgaa gttgtacaag
    47341 ttgttccaga aaagaacgtt atccttatca aaggtaacgt accaggtgct aagaaatctc
    47401 ttatcactat caaatcagca gttaaagctg gtaaataata aagaaagggg aaatcagtca
    47461 caatggcaaa cgtaacatta tttgaccaaa ctggtaaaga agctggccaa gttgttctta
    47521 gcgatgcagt atttggtatc gaaccaaatg aatcagttgt gtttgatgta atcatcagcc
    47581 aacgcgcaag ccttcgtcaa ggaacacacg ctgttaaaaa ccgctctgca gtatcaggtg
    47641 gtggacgcaa accatggcgt caaaaaggaa ctggacgtgc tcgtcaaggt tctatccgct
    47701 caccacaatg gcgtggtggt ggtgttgtct tcggaccaac tccacgttca tacggctaca
    47761 aacttccaca aaaagttcgt cgcctagctc ttaaatcagt ttactctgaa aaagttgctg
    47821 aaaacaaatt cgtagctgta gacgctcttt catttacagc tccaaaaact gctgaatttg
    47881 caaaagttct tgcagcattg agcatcgatt ctaaagttct tgttatcctt gaagaaggaa
    47941 atgaattcgc agctctttca gctcgtaacc ttccaaacgt gaaagttgca actgctacaa
    48001 ctgcaagtgt tcttgacatc gcaaatagcg acaaacttct tgtcacacaa gcagctatct
    48061 ctaaaatcga ggaggttctt gcataatgaa tttgtatgat gttatcaaaa aacctgtcat
    48121 cactgaaagc tcaatggctc aacttgaagc agggaaatat gtatttgaag ttgacactcg
    48181 tgcacacaaa cttttgatca agcaagctgt tgaagctgct ttcgaaggtg ttaaagttgc
    48241 caatgttaac acaatcaacg taaaaccaaa agctaaacgt gttggacgtt acactggttt
    48301 tactaacaaa actaaaaaag ctatcatcac acttacagct gattctaaag caatcgagtt
    48361 gtttgctgct gaagctgaat aatctaagga ggaaatatcg tgggaattcg tgtttataaa
    48421 ccaacaacaa acggtcgccg taatatgact tctttggatt tcgctgaaat cacaacaagc
    48481 actcctgaaa aatcattgct tgttgcattg aagagcaagg ctggtcgtaa caacaacggt
    48541 cgtatcacag ttcgtcacca aggtggtgga cacaaacgtt tttaccgttt ggttgacttc
    48601 aaacgtaata aagacaacgt tgaagcagtt gttaaaacaa tcgagtacga tccaaaccgt
    48661 tctgcaaaca tcgctcttgt acactacact gacggtgtga aagcatacat catcgctcca
    48721 aaaggtcttg aagtaggtca acgtatcgtt tcaggtccag aagcagatat caaagtcgga
    48781 aacgctcttc cacttgctaa catcccagtt ggtactttga ttcacaacat cgagttgaaa
    48841 ccaggtcgtg gtggtgaatt ggtacgtgct gctggtgcat ctgctcaagt attgggttct
    48901 gaaggtaaat atgttcttgt tcgtcttcaa tcaggtgaag ttcgtatgat tcttggaact
    48961 tgccgtgcta cagttggtgt tgtcggaaac gaacaacatg gacttgtaaa ccttggtaaa
    49021 gcaggacgta gccgttggaa aggtatccgc ccaacagttc gtggttctgt aatgaaccct
    49081 aacgatcacc cacacggtgg tggtgaaggt aaagcaccag ttggtcgtaa agcaccatct
    49141 actccatggg gcaaacctgc tcttggtctt aaaactcgta acaagaaagc gaaatctgac
    49201 aaacttatcg ttcgtcgtcg caacgagaaa taatattaaa ctagtcgctt aagcaactag
    49261 taaatccgcc agctcggtag cgctccatag gagtgcaagc cgctgtggta caacatttaa
    49321 aggagaaaat ataaaaatgg gacgcagtct taaaaaagga cctttcgtcg atgagcattt
    49381 gatgaaaaaa gttgaagctc aagctaacga cgaaaagaaa aaagttatta aaacttggtc
    49441 acgtcgttca acgatcttcc caagtttcat tggttacact attgcagttt atgacggacg
    49501 taaacacgta cctgtttaca tccaagaaga catggtaggc cacaaacttg gtgaatttgc
    49561 accaactcgt acttacaaag gtcacgctgc agacgacaag aaaacacgta gaaaataagg
    49621 agaacataaa tggcagaaat tacttcagct aaagcaatgg ctcgtacagt acgtgtttca
    49681 cctcgtaaat cacgtcttgt tcttgataac atccgtggta aaagcgtagc cgatgcaatc
    49741 gcaatcttga cattcactcc aaacaaagct gctgaaatca tcttgaaagt tttgaactca
    49801 gctgtagcta acgctgaaaa caactttggt ttggataaag ctaatttggt agtatctgaa
    49861 gcattcgcaa acgaaggacc aactatgaaa cgtttccgtc cacgtgcgaa aggttcagct
    49921 tcaccaatca acaaacgtac agctcacatc actgtagctg ttgcagaaaa ataaggaggt
    49981 aaaatcgtgg gtcaaaaagt acatccaatt ggtatgcgtg tcggcatcat ccgtgattgg
    50041 gatgccaaat ggtatgctga aaaagaatac gcggattacc ttcatgaaga tcttgcaatc
    50101 cgtaaattcg ttcaaaaaga acttgctgac gcagcagttt caactattga aatcgaacgc
    50161 gcagtaaaca aagttaacgt ttcacttcac actgctaaac caggtatggt tatcggtaaa
    50221 ggtggtgcta acgttgatgc actccgtgca aaacttaaca aattgactgg aaaacaagta
    50281 cacatcaaca tcatcgaaat caaacaacct gatttggatg ctcaccttgt aggtgaagga
    50341 attgctcgtc aattggagca acgtgttgct ttccgtcgtg cacaaaaaca agcaatccaa
    50401 cgtgcaatgc gtgctggagc taaaggaatc aaaactcaag tatcaggtcg tttgaacggt
    50461 gcagatatcg cccgtgctga aggatactct gaaggaactg ttccgcttca cacacttcgt
    50521 gcagatatcg attacgcttg ggaagaagca gatactacat acggtaaact tggtgttaaa
    50581 gtatggatct accgtggtga agttcttcca gctcgtaaaa acactaaagg aggtaaataa
    50641 ccaatgttag tacctaaacg tgttaaacac cgtcgtgagt tccgtggaaa aatgcgcggt
    50701 gaagcaaaag gtggaaaaga agtagcattc ggtgaatacg gtcttcaagc tacaactagc
    50761 cactggatca ctaaccgcca aatcgaagct gctcgtatcg ccatgactcg ttacatgaaa
    50821 cgtggtggta aagtttggat taaaatcttc ccacacaaat catacactgc taaagctatc
    50881 ggtgtgcgta tgggatctgg taaaggggca cctgaaggtt gggtagcacc agttaaacgt
    50941 ggtaaagtga tgttcgaaat cgctggtgta tctgaagaga ttgcacgtga agcgcttcga
    51001 cttgctagcc acaaattgcc agttaaatgt aaattcgtaa aacgtgaagc agaataagga
    51061 gaaggcatga aacttaatga agtaaaagaa tttgttaaag aacttcgtgg tctttctcaa
    51121 gaagaactcg cgaagcgcga aaacgaattg aaaaaagaat tgtttgaact tcgtttccaa
    51181 gctgctactg gtcaattgga acaaacagct cgcttgaaag aagttaaaaa acaaatcgct
    51241 cgtatcaaaa cagttcaatc tgaagcgaaa taatagacta gggaaggaga aatttcaatg
    51301 gaacgcaata atcgtaaagt tcttgttgga cgtgttgtat ctgacaaaat ggacaagaca
    51361 atcacagttg tagttgaaac aaaacgtaac cacccagtct atggtaaacg tattaactac
    51421 tctaaaaaat acaaagctca tgatgaaaac aatgttgcca aagaaggcga tatcgtacgt
    51481 atcatggaaa ctcgcccgct ttcagctaca aaacgtttcc gtcttgtaga agttgttgaa
    51541 gaagcggtca tcatctaatc aaacctgaaa ggagaaaact gaaatgattc aaacagaaac
    51601 tcgtttgaaa gtcgcagaca acagcggtgc tcgcgaaatc ttgactatca aagttcttgg
    51661 tggttcagga cgtaaatttg caaacatcgg tgatgttatc gtggcatctg taaaacaagc
    51721 tactcctggt ggtgcggtta aaaaaggtga cgttgttaaa gcagttatcg ttcgtactaa
    51781 atcaggtgct cgtcgtgctg atggttcata catcaaattt gacgaaaacg cagcagttat
    51841 catccgtgaa gacaaaactc ctcgcggaac acgtatcttt ggcccagttg cacgtgaatt
    51901 gcgtgaaggt ggcttcatga agatcgtgtc acttgctcca gaagtacttt aatttttagg
    51961 aacaaactag tcccctagct tcaagctagg gtgcccttat gggcgtaaga aaaatcaagg
    52021 agaaacctaa tgtttgtaaa aaaaggcgac aaagttcgcg taatcgctgg taaagataag
    52081 ggaacagaag ctgttgtcct tactgccctt ccaaaagtaa acaaagttat cgttgaaggt
    52141 gttaacattg ttaagaaaca ccaacgtcca actaacgagc ttcctcaagg tggtatcatc
    52201 gagaaagaag cagctatcca cgtatcaaac gttcaagttt tggacaaaaa tggtgtagct
    52261 ggtcgtgttg gttacaaatt tgtagacggt aaaaaagttc gctacaacaa aaaatcaggc
    52321 gaagtgcttg attaatcacg aaggaaagga gaagtataat ggcaaatcgt ttaaaagaaa
    52381 aatatcttaa tgaagtagtt cctgctttga cagaacaatt caactactca tcagtgatgg
    52441 ctgtgcctaa agtagataag attgttttga acatgggtgt tggtgaagct gtatcaaacg
    52501 ctaaaagcct tgaaaaagct gctgaagaat tggcacttat ctcaggtcaa aaaccactta
    52561 tcactaaagc taaaaaatca atcgccggct tccgtcttcg tgaaggtgtt gcgatcggtg
    52621 caaaagttac ccttcgtggt gaacgtatgt acgaattctt ggataaattg gtatcagttt
    52681 cacttccacg tgtacgtgac ttccacggtg ttccaacaaa atcatttgat ggacgcggga
    52741 actacacact tggtgtgaaa gaacaattaa tcttcccaga aatcaacttc gatgacgttg
    52801 acaaaactcg tggtcttgac atcgttatcg taacaactgc taacactgac gaagagtcac
    52861 gtgcattgct tacaggcctt ggaatgcctt ttgcaaaata atataggagg taaatctaat
    52921 ggctaaaaaa tcaatggtag ctagagaggc taaacgccaa aaaattgttg accgttatgc
    52981 tgaaaaacgt gctgcattaa aggcggcagg ggactacgaa ggtttatcta aattacctcg
    53041 caacgcttca ccgactcgtt tacataatcg ttgtagagtt acggggcgtc cacattcagt
    53101 ttaccgcaaa tttggtctga gtcgtatcgc ttttcgcgaa cttgcgcata aaggtcaaat
    53161 tcctggtgta acaaaagcat cttggtaatt taagatatca agggcgtcaa aactccaagt
    53221 aaaaatagga aacttgacga agaaactaaa gtttctagga aagtttatct ttttcacaca
    53281 gagtttagcc cgggttcaat tgggcttgcc aatttgaaca cgagctacag ctttggcaaa
    53341 aaagaccaat ttgctttgga gcattgcttc tgcattaaat tgtctatttt tgctcgtgct
    53401 gttacgctct ttgtatcatg tattaactag caagtgcaac ttgcaaacta ctagtaagag
    53461 gagaaaaaca aaatggttat gactgaccca atcgcagact tcctaactcg tattcgtaat
    53521 gctaaccaag ctaaacacga agtacttgaa gtacctgcat caaacatcaa aaaagggatt
    53581 gctgaaatcc ttaaacgcga aggttttgta aaaaacgttg aaatcatcga agatgacaaa
    53641 caaggcgtca tccgtgtatt tcttaaatac ggaccaaatg gtgaaaaagt tatcactaac
    53701 ttgaaacgtg tttctaaacc aggacttcgt gtctacaaaa aacgtgaaga ccttccaaaa
    53761 gttcttaacg gacttggaat tgccatcctt tcaacttctg aaggtttgct tactgataaa
    53821 gaagcacgcc aaaagaatgt tggtggtgag gttatcgctt acgtttggta aaatcaagat
    53881 acaaagctcg taaagaacaa agcaaaatta ggaagttgga gaagtttgtt tacaaacaag
    53941 ccaacttatc tattttgcac agttcttaga gcgtgttcag ttcagctctt gaactaaata
    54001 agtatctgaa ccccgtgaaa actggccgtt ttggcctgac aatttaacag gagaaaataa
    54061 acatgtcacg tattggtaat aaagttatcg tgttgcctgc tggtgttgaa ctcgctaaca
    54121 atgacaacgt tgtaactgta aaaggaccta aaggagaact tactcgtgag ttctcaaaag
    54181 atattgaaat ccgtgtggaa ggtactgaag taactcttca ccgtccaaac gattcaaaag
    54241 aaatgaaaac tatccacgga actactcgtg cccttttgaa caacatggtt gttggtgtat
    54301 cagaaggatt caagaaagaa cttgaaatgc gtggggttgg ttaccgtgca cagcttcaag
    54361 gatctaaact tgttttggct gttggtaaat ctcatccaga cgaagttgaa gctccagaag
    54421 gaattacttt tgaacttcca aacccaacaa caatcgttgt tagcggaatt tcaaaagaag
    54481 tagttggtca aacagctgct tacgtacgta gccttcgttc accagaacca tataaaggta
    54541 aaggtatccg ttacgttggt gaattcgttc gccgtaaaga aggtaaaaca ggtaaataat
    54601 gttgagtggt tgatcatcaa ccaccaacct attttccaac tttgtgcata gcacacgatt
    54661 taaaactaaa gaggtgaaaa ctgtgatttc aaaaccagat aaaaacaaac tccgccaaaa
    54721 acgccaccgt cgcgttcgcg gaaaactctc tggaactgct gatcgcccac gtttgaacgt
    54781 attccgttct aatacaggca tctacgctca agtgattgat gacgtagcgg gtgtaacgct
    54841 cgcaagtgct tcaactcttg ataaagaagt ttcaaaagga actaaaactg aacaagccgt
    54901 tgctgtcggt aaactcgttg cagaacgtgc aaacgctaaa ggtatttcag aagtggtgtt
    54961 cgaccgcggt ggatatctat atcacggacg tgtgaaagct ttggctgatg cagctcgtga
    55021 aaacggattg aaattctaat aggaggacac tagaaaatgg catttaaaga caatgcagtt
    55081 gaattagaag aacgcgtagt tgctgtcaac cgtgttacaa aagttgttaa aggtggacgt
    55141 cgtcttcgtt tcgcagctct tgttgttgtt ggtgaccaca atggtcgcgt aggatttggt
    55201 actggtaaag ctcaagaagt tccagaagca atccgtaaag cagtagatga tgctaagaaa
    55261 aacttgatcg aagttcctat ggttggaaca acaatcccac acgaagttct ttcagaattc
    55321 ggtggagcta aagtattgtt gaaacctgct gtagaaggtt ctggagttgc cgctggtggt
    55381 gcagttcgtg ccgttgtgga attggcaggt gtggcagata ttacatctaa atcacttggt
    55441 tctaacactc caatcaacat tgttcgtgca actgttgaag gtttgaaaca attgaaacgc
    55501 gctgaagaaa ttgctgccct tcgtggtatt tcagtttctg atttggcata agaaagggga
    55561 taaaatggct caaattaaaa ttactttgac taagtctcca atcggacgca ttccatcaca
    55621 acgtaaaact gttgtagcac ttggacttgg caaattgaac agctctgtta ttaaagaaga
    55681 taacgctgct atccgtggta tgattacagc agtatctcac ttagtaacag ttgaagaagt
    55741 aaactaatga agttttaggg gatgtgcact gtaccatccc ctaaaactag atatagtcat
    55801 ctatgatgac atcgtatagg cgagttgatg ggggagacaa ccttttctcc cttatcggcg
    55861 ctagcatttt acaaaagagg agaaaataaa aatgaaactt catgaattga aacctgcaga
    55921 aggttctcgt aaagtacgta accgcgttgg tcgtggtact tcatcaggta acggtaaaac
    55981 atctggtcgt ggtcaaaaag gtcaaaaagc tcgtagcggt ggcggagttc gccttggttt
    56041 tgaaggtgga caaactccat tgttccgtcg tcttccaaaa cgtggattca ctaacatcaa
    56101 cgctaaagaa tacgcaattg tgaaccttga ccaattgaac gtctttgaag atggtgctga
    56161 agtaactcca gttgttctta tcgaagcagg aattgttaaa gctgaaaagt caggtattaa
    56221 aattcttggt aacggtgagt tgactaagaa attgactgtg aaagcagcta aattctctaa
    56281 atcagctgaa gaagctatca ctgctaaagg tggttcagta gaagtcatct aagagaggtg
    56341 acctatgttt tttaaattat taagagaagc tcttaaagtc aagcaggttc gatcaaaaat
    56401 tttatttaca atttttatcg ttttggtctt tcgtatcgga actagcatta cagttcctgg
    56461 tgtgaatgcc aatagcttga atgctttaag tggattatcc ttcttaaaca tgttgagctt
    56521 ggtgtcgggg aatgccctaa aaaacttttc gatttttgcc ctaggagtta gtccctatat
    56581 caccgcttct attgttgtcc aactcttgca aatggatatt ttacccaagt ttgtagagtg
    56641 gggtaaacaa ggggaagtag gtcgaagaaa attgaatcaa gctactcgtt atattgctct
    56701 agttctcgct tttgtgcaat ctatcgggat tacagctggt tttaatacct tggctggagc
    56761 tcaattgatt aaaactgctt taactccaca agtttttctg acgattggta tcatcttaac
    56821 agctggtagt atgattgtca cttggttggg tgagcaaatt acagataagg gatacggaaa
    56881 cggtgtttcc atgattatct ttgccgggat tgtttcctca attccagaga tgattcaggg
    56941 catctatgtg gactactttg tgaacgtccc aagtagccgt atcacttcat ctatcatttt
    57001 cgtaatcatt ttgattatta ctgtattgtt gattatttac tttacaactt atgttcaaca
    57061 agcagaatac aaaattccaa tccaatatac taaggttgca caaggtgctc catctagctc
    57121 ttaccttccg ttaaaggtaa accctgctgg agttatccct gttatctttg ccagttcgat
    57181 tactgcagcg cctgcggcta ttcttcagtt tttgagtgcc acaggtcatg attgggcttg
    57241 ggtaagggta gcacaagaga tgttggcaac tacttctcca actggtattg ccatgtatgc
    57301 tttgttgatt attctcttta cattcttcta tacgtttgta cagattaatc ctgaaaaagc
    57361 agcagagagc ctacaaaaga gtggtgccta tatccatgga gttcgtcctg gtaaaggtac
    57421 agaagaatat atgtctaaac ttcttcgtcg tcttgcaact gttggttccc tcttccttgg
    57481 tgtgatttcc attttaccga ttgcagctaa agatgtattt ggtctttctg atgttgttgc
    57541 ctttggtgga acaagtctct tgatcattat ctctacaggt atcgaaggaa tcaagcaatt
    57601 ggaaggttac ctattgaaac gtaagtatgt tggtttcatg gacagaacag aataaaagta
    57661 tttactgaat cagtaaatac tgagggagtg gaggtttaaa ctctgacatt tgtaagagtt
    57721 ggatctcccc tcttctattt tgtttttaaa taggggtgaa aagacttttt gcttctattt
    57781 aaaaataaaa taaggagatc aaatcatgaa tcttttgatt atgggcttac ctggtgcagg
    57841 taagggaact caagcagcaa aaatcgtaga acaattccat gttgcacata tctcaacagg
    57901 tgatatgttc cgcgctgcaa tggcaaatca aactgaaatg ggtgttcttg ctaagtcata
    57961 tattgacaag ggtgaattgg ttcctgacga agttacaaat ggaatcgtaa aagaacgcct
    58021 ttcacaagat gatattaaag aaacaggatt cttattggat ggttacccac gtacaattga
    58081 acaagctcat gccttggaca aaacattggc tgaacttggc attgaactag aaggtattat
    58141 caatattgaa gtgaaccctg acagcctctt ggaacgtttg agtggccgta tcatccaccg
    58201 cgtaactgga gaaactttcc acaaggtctt taacccacca gttgactata aagaagaaga
    58261 ttactaccaa cgtgaagatg ataagcctga gacagtaaaa cgtcgtttgg atgttaatat
    58321 tgctcaagga gaaccaatca ttgctcacta ccgtgccaaa ggtttggttc atgacatcga
    58381 aggtaatcaa gatatcaatg atgtcttctc agatattgaa aaagtattga caaatttgaa
    58441 ataaagcgtt tttcgcactt gcaaaaatcc gctacaaatg ttatactgag atagtctgac
    58501 ttataattgt tgtctctgtg tctagaggca tcgaatcgaa atttatggag gtgcttttgc
    58561 gtggcaaaag acgatgtgat tgaagttgaa ggcaaagtag ttgatacaat gccgaatgca
    58621 atgtttacgg ttgaacttga aaatggacat cagattttag caacagtttc tggtaaaatt
    58681 cgtaaaaact atattcgtat tttagcggga gatcgtgtta ctgtcgaaat gagtccatat
    58741 gacttgacac gtggacgtat cacttaccgc tttaaataat cgaaaaactt ggagggataa
    58801 gaaatgaaag taagaccatc ggtcaaacca atttgcgaat actgtaaagt tattcgtcgt
    58861 aatggtcgtg ttatggtaat ttgcccagca aatccaaaac acaaacaacg tcaaggataa
    58921 gatagaaagg agaaaacatg gctcgtattg ctggagttga tattccaaat gacaaacgcg
    58981 tagtaatctc attgacttat gtttatggta tcggacttgc aacatctaag aaaattttgg
    59041 ctgctgctgg aatctcagaa gatgttcgtg tacgtgatct tacatcagat caagaagatg
    59101 ctatccgtcg tgaagtggat gcaatcaaag ttgaaggtga ccttcgtcgt gaagtaaact
    59161 tgaacatcaa acgtttgatg gaaatcggtt cataccgtgg tatccgtcac cgtcgtggac
    59221 ttcctgtccg tggacaaaac actaaaaaca acgctcgcac tcgtaaaggt aaagctgttg
    59281 cgattgctgg taagaaaaaa taatatagga ggtaaaagtc ttggctaaac caacacgtaa
    59341 acgtcgtgtg aaaaagaata tcgaatctgg tattgctcat attcacgcta catttaataa
    59401 cactattgtt atgattactg atgtgcatgg taatgcaatt gcttggtcat cagctggtgc
    59461 tcttggtttc aaaggttctc gtaaatctac accattcgct gctcaaatgg cttctgaagc
    59521 tgctgctaaa tctgcacaag aacacggtct taaatcagtt gaagttactg taaaaggtcc
    59581 aggttctggt cgtgagtcag ctattcgtgc gcttgctgcc gctggtcttg aagtaacagc
    59641 aattcgtgat gtgactccag tgccacacaa tggtgctcgt cctccaaaac gtcgccgtgt
    59701 ataatcatcg cattacactg cttttcgttt aagagggagt aactaaatga tcgagtttga
    59761 aaaaccaaat ataacaaaaa ttgatgaaaa taaagattat ggcaagtttg taatcgaacc
    59821 acttgaacgt ggctacggta caactcttgg taactctctt cgtcgtgtac ttctagcttc
    59881 tctaccagga gcagctgtga catctatcaa cattgatggt gtgttacatg agtttgacac
    59941 agttccaggt gttcgtgaag acgtgatgca aatcattctg aacattaaag gaattgcagt
    60001 gaaatcgtac gttgaagacg aaaaaatcat cgaactggat gttgaaggtc ctgctgaagt
    60061 aacagctggt gacattttga cagatagcga tattgaaatt gtaaatccag atcattatct
    60121 ctttacaatc ggtgaaggtt cttctctaaa agcgactatg actgttaaca gtggtcgtgg
    60181 atatgtacct gctgatgaaa ataaaaagga taatgcacca gttggaacac ttgctgtaga
    60241 ttctatttat acaccagtta caaaagtcaa ctatcaagtg gaacctgctc gtgtaggtag
    60301 caatgatggt ttcgacaaat taacccttga aatcttgaca aatggaacaa ttattccaga
    60361 agatgcttta gggctttcag cacgtatttt gacagaacat cttgatttgt ttacaaatct
    60421 tactgagatt gctaagtcaa ctgaagtgat gaaagaagct gatactgaat ctgacgaccg
    60481 tattttagat cgtacgattg aggaactgga cttgtctgtg cgttcataca actgtttaaa
    60541 acgtgccggt atcaatactg tgcatgattt gacagaaaaa tctgaagcag agatgatgaa
    60601 agtacgaaat cttggacgca agagtttgga agaagtgaaa ctcaaactca ttgatttggg
    60661 tcttggatta aaagataaat aaaggaggaa tacatggctt accgtaaact aggacgcact
    60721 agctcacaac gtaaagcaat gcttcgcgat ttgacaactg accttttgat caacgaatca
    60781 atcgtgacaa ctgaagctcg tgctaaagaa atccgtaaaa ctgttgaaaa aatgattact
    60841 ctaggtaaac gtggtgattt gcatgcacgt cgtcaagcag ctgctttcgt acgtaatgaa
    60901 atcgcatctg aaaactatga tgaagcaact gataagtaca cttctactac agcacttcaa
    60961 aaattgttct cagaaatcgc acctcgttat gctgaacgta acggtggata cactcgtatc
    61021 cttaaaactg aaccacgtcg tggtgatgca gcgccaatgg cgatcatcga attagtataa
    61081 aatcatcaat tttgttgagt gttatgatga tggagtcttg tgctcttagt ctagctctgg
    61141 tctaccgcta ggatttcggt cctagcggga acactcatca taagttggga tagtagacgc
    61201 ttgtttacga aattgttttt ttcttaagaa caacttcgta agcaggcgtt tttgagtatt
    61261 ttcgttagaa ttatgctata ctatttgaaa agaatcctgt ttaatgttaa ggtttcttat
    61321 tttaagaaga attggagttt acttatgaaa gccattataa ctgttgttgg taaagataaa
    61381 tctggaattg ttgcaggtgt ttctggtaaa attgcagaat taggattgaa tattgacgat
    61441 atctctcaaa ctgtcttgga tgaatatttt acgatgatgg ctgttgtatc tagtgatgaa
    61501 aagcaagatt ttacctatct tcgtaatgaa tttgaagctt ttgggcaaac tttgaatgta
    61561 aaaatcaata ttcagagtgc agcgattttc gaagctatgt ataatatcta ggaggttatc
    61621 atggatatta gacaagttac tgaaaccatc gccatgattg aggagcaaaa tttcgatatc
    61681 agaaccatta ccatggggat ttctcttttg gattgtatcg atccagatat caatcgtgct
    61741 gcggagaaaa tctatcaaaa aattacgaca aaggcggcta atttagtagc tgttggtgat
    61801 gaaattgcgg ctgagttggg aattcctatc gttaataagc gtgtatcggt gacacctatt
    61861 tctctgattg gggcagcgac agatgcgacg gactacgtgg ttctggcaaa agcgcttgat
    61921 aaggctgcga aagagattgg tgtggacttt attggtggtt tttctgcctt agtacaaaaa
    61981 ggttatcaaa agggagatga gattctcatc aattccattc ctcgcgcttt gactgagacg
    62041 gataaggtct gctcgtcagt caatatcggc tcaaccaagt ctggtattaa tatgacggct
    62101 gtggcagata tgggacgaat tatcaaggaa acggcaaatc tttcagatat gggagcggcc
    62161 aagttggttg tattcgctaa tgctgttgag gacaatccat ttatggcggg tgcctttcat
    62221 ggtgttgggg aagcagatgt tatcatcaat gtcggagttt ctggtcctgg tgtggtgaaa
    62281 cgtgctttgg aaaaagttcg tggacagagc tttgatgtag tagccgaaac agttaagaaa
    62341 actgccttta aaatcactcg tatcggtcaa ttggttggtc aaatggccag tgagagactg
    62401 ggtgtggagt ttggtattgt ggacttgagt ttggcaccaa cccctgcggt tggagactct
    62461 gtggcacgtg tccttgagga aatggggcta gaaacagttg gcacgcatgg aacgacagct
    62521 gccttggccc tcttgaacga ccaagttaaa aagggtggag tgatggcctg taatcaggtc
    62581 ggtggtctat ctggtgcctt tatccctgtt tctgaggatg aaggaatgat tgctgcagtg
    62641 caaaatggct ctcttaattt agaaaaacta gaagctatga cggctatctg ttctgttgga
    62701 ttggatatga ttgccatccc agaagatacg cctgctgaaa ctattgcggc tatgattgcg
    62761 gatgaagcag caatcggtgt tatcaacatg aaaacaacag ctgttcgtat cattcccaaa
    62821 ggaagagaag gcgatatgat tgagtttggt ggtctattag gaactgcacc cgttatgaag
    62881 gttaatgggg cttcgtctgt cgacttcatc tctcgcggtg gacaaatccc agcaccaatt
    62941 catagtttta aaaattaaga aaataggaga aattttaagt tctatttaag attagatgtg
    63001 tatactataa tcattaaata aagacctcct aatattattt gaaacagata acactgaatt
    63061 agtttgaatt tgattttcat ctaatatctt tatttaatga actcctaaac tttttcataa
    63121 taatctcctt caaaagtcgc ctgtatgggt ggcttttatt ttatcattca tgatataata
    63181 gaagcaaacg gaggacggaa aatggtaaaa gtacgattgt atttgatacg tcatggcaag
    63241 accatgttta acacgattgg tcgcgcgcaa ggttggagcg atactccctt aactgctgaa
    63301 ggtgaacgag ggattcaaga gttaggaatt ggtttgcgag aatctgatct acagtttgag
    63361 cgcgcttatt cgagtgattc tggtcgtacc attcagacca tgggaattat ccttgaagaa
    63421 cttggcttgc aggggaaaat cccttatcgc atggacaagc gtatcagaga atggtgtttc
    63481 ggtagttttg atggagccta tgatggcgat cttttcatgg gcattattcc tcgtatcttt
    63541 aatgtggacc acgttcacca attgtcttat gctgaactgg ctgagggctt ggtagaggtc
    63601 gatacagctg gttgggctga aggctgggaa aaactcagtg gccgaatcaa ggaaggcttt
    63661 gaaatgattg caaaagaaat ggaagatcaa ggtggaggta acgcccttgt tgtcagccat
    63721 ggaatgacta ttggaaccat tgtttatctg attaatggca tgcatccgca tggtctagat
    63781 aatggtagcg tgacgattct tgaatatgag gacggtcagt ttagggttga agttgtcggt
    63841 gaccgtagtt accgagagct aggacgtgag aagatggaag aaggctctat ttaatcagtc
    63901 tagacttgct tgccatgagc tagggatttg ataagaatat caagataaga aaaaacagcc
    63961 gagggtactc ctttcggctg tttttgatgt ggaaaactaa agtgtaatgc tattgctttt
    64021 agagattttc ataaacaaga gcaaggaacc tactgttaga acagtcagga tagttgacaa
    64081 ggctgcggct acaccgtaat ttcctctgag aacctctgta taaatagcta cagtcattgt
    64141 tcttgttttg acattgtaga ggaggataga agtagagagt tctgaaatca gtgtgaccca
    64201 agataggatg gctccagaaa taatatccga tagcatcatt ggagttgtaa tcttagcaaa
    64261 gatattgaga cgactacttc ctaagctttc agcagcttct tcaatacttg gtgctatttg
    64321 ttgtaagcta gcaacagatg agcgaatagt ataaggtaat tttctggcag atagagacat
    64381 aatcaagatg aaagcagtcc ctgtaatcat aagaaatcca cttccaaata gaccagagaa
    64441 atgaaggcaa tccctagaac ggttcctggt acaatataag gtaccatact gaggctgtca
    64501 attaagtttg taaacaaatt ccgttttcta acggctaggt aggagataaa tgtcgcaaat
    64561 agaacaacta gaactaaggc aatcaaaggg atacgaatgg tattgaaaat agcagatccc
    64621 atacgatgga aagctacctt gtaactgttt ggagaataac ctttaacaga taccatacct
    64681 gatgttttta ggaaagaggt ataaattaag tagatttgag gtaaaacaga gataaagata
    64741 attccgtaga ctgttgcata aatggcagct atttttcctt ttgtagtttt tttaggctca
    64801 attggatgga gcagattcat gctgaaactg tagcggtttg caatgtgttt ttggataagg
    64861 aaaattgcca aggcaatgat aatcgccata attgcaaaag cagaatttcc tccaacctcg
    64921 ctaataaatt gggtataaat caggacaggg aaagtccgat atccttcgcc aatcaacata
    64981 ggcgttccaa agtctgagaa tgctctcata aatacaagca aggagctgct agtaaggttg
    65041 gaactaggag aggtaaaaca accgttacga tacgcttaaa tccgaaggac ccccatgctt
    65101 tcagctgctt caagtagaga attgtcaata ctgttcattg ttccagcaac atatagaaat
    65161 accagtggga atagttgcag tgtaaagaca agtacaattc ctttgaatcc ataaatatcg
    65221 atagctggaa gatgaagggc atttgtcaaa aatttagtga tgaccccatt tcgtcctagc
    65281 aagagaaccc aggagtaggc tcctacgaaa ggagctgaca tggaagcaat gataatcaat
    65341 atttgtagaa atttctcccc cttgaagtca tacatagaga agagataagc taatagggtt
    65401 cctacaacta aggaagtgac agtagcggta atggaaacct tgaaactgtt gactagtgtc
    65461 tcagagtagt agactttact aaagaaagtg acaaaattag ctagtgagaa ttatccttca
    65521 tgtataagtg cttacttgag cacggtaatg ataggataaa cgagaaagac aagataggta
    65581 agaaagatga agaaagagga ggctgtccaa atatttagtt ttttacattc catggttgac
    65641 tccttttatc aggttttggg aaccatctgc agaaaagatg tttaattttt gcgtattgat
    65701 tcgtagacga atacgattgt ctttttgtag atcttcttca aaagttgatt cttcactaac
    65761 ttgaattttt gaggcaaaac ctgtctcaat gaaatactcc gtatttagtc caagatagac
    65821 gctatcgcta atagttcctt caatatctcc agattcatct ttgataaact cttcgggacg
    65881 aatgcttaca tgaatagctt gctcctcaac ctgatcaaga gctggcattc gaagggcata
    65941 gccatctgaa aagacgatat aagcgccgtc gctccgtttt tcaagattgg cagggataat
    66001 atttgtgcgt ccgataaagg ttgccacaaa ctcattagct ggtttatgat agagttcttt
    66061 tggtcggccg atttgttgga tcaccccatc tttcataaca gcaatttgat ctgaaatagc
    66121 catggcttct tcttggtcgt gggttacata aacagttgta attcccactt cgtgttggat
    66181 ttctcggatg gcttgacgca tatccaagcg aagtttggcc tccagattac taagtggctc
    66241 gtccatgagg agaacacttg gattaaccgc taaggcgcat gccaaggtga cacgttgttg
    66301 ttgtccacca ctgagtttat cgggctttcg atccgcatat tgagcaattt gcatgagttc
    66361 aagatacttg ttggtctgtt gaatcaattc ttcttttgga accttctttt gcttaagacc
    66421 aaaagcaacg ttgtctcgga cagtcaaatg tgggaaaata gcgtagtttt ggaaaaccat
    66481 cccgatattg cgtttgctgg gttccatatt attgattttt gtatcatcga agtaaaattc
    66541 tccatctttg atactgttga aacctgcaat catacgaaga agggtcgttt tcccacatcc
    66601 tgaaggtcca agaagggtaa agagacttcc ttttggaatt gtaatgttca aattctcaat
    66661 aacagggaca tcgtggtaga ttttttggcg ttaataattt tgatctcact catagtgaac
    66721 cttttttact gtttagattg gatatctgta aagacttcgt tgtatttctt aacgatatct
    66781 gatttattct tgatgacata atcataatct tcagtgagtg ttttgatttt gtcaattggt
    66841 ttcatgtttt cgcttgtttt agcattttta cgaacaggac ggttagtagt ggttgtacca
    66901 agtgtatctt gtacttcttg agagataata aaatcgataa atttcttgtc attttccata
    66961 tttttagctt ttttaacgat agcagcacta gcaggtagga agacggttcc ttcttttgga
    67021 tagactactt taatgttagc tccgtcattt aagagtttaa ctgctggatc ctcataagag
    67081 agaccaacag ccatttctcc atcaacgact gctttataga caccagatga acttgaaccg
    67141 attttatagt gtattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat
    67201 cgatttgact gtcctgatta atttgtcctg ttcttatttc atttcactat gccatcaata
    67261 agtgtgaaaa gatcttttac ataagaccaa gccttatcat ctttgtaacc accttgagct
    67321 tgtagcatat ttgttaattg agcaaaggcg ctagaagagt ttgctgggtc agcagttgcg
    67381 atttttcctt ttagttcagg tttgagaagg tcgctatatc cttcgatgtt catgccttta
    67441 gttaaatcag ggttgacgat taaaacacta ccatctagtg tataaggagt agagaagcca
    67501 gttgtgtttt gatattcttt gataacatta tcattttctt ttgaaatata gttttcaaag
    67561 agttctccgt gggtagcata ttgtgtataa gaaccaccaa agataacatc agctacagga
    67621 acttcttttt ctgactctag ttttttgaaa agttctccag taccagcttg aatcagttct
    67681 attttgatac catatttttc ttcaaaggca ggaatagttg ctccaattaa gccttctgag
    67741 tttggtgaat aaacgactag cgaaccaccg tctcctttat cagatgaact gtcatcggca
    67801 gattcattag aagaacaagc agcataatac atctatttct ttttcatgat ggatatctcc
    67861 gttgtgttat ttaagtttat tttaaaacaa tgtaagcgtt tttaaaacct acaattcta
//
LOCUS       DAVLFB010000002        65211 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLFB010000002 DAVLFB010000000
VERSION     DAVLFB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.002"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            127..1152
                     /gene="trpS"
                     /locus_tag="VXD58_000084"
     CDS             127..1152
                     /gene="trpS"
                     /locus_tag="VXD58_000084"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV0674851.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1304..2782
                     /gene="guaB"
                     /locus_tag="VXD58_000085"
     CDS             1304..2782
                     /gene="guaB"
                     /locus_tag="VXD58_000085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV0674852.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2833..3930)
                     /gene="recF"
                     /locus_tag="VXD58_000086"
     CDS             complement(2833..3930)
                     /gene="recF"
                     /locus_tag="VXD58_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV0674853.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3933..4301)
                     /gene="yaaA"
                     /locus_tag="VXD58_000087"
     CDS             complement(3933..4301)
                     /gene="yaaA"
                     /locus_tag="VXD58_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV0674854.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4460..5710
                     /locus_tag="VXD58_000088"
     CDS             4460..5710
                     /locus_tag="VXD58_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0674855.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5707..6990
                     /locus_tag="VXD58_000089"
     CDS             5707..6990
                     /locus_tag="VXD58_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV0674856.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7032..7853
                     /locus_tag="VXD58_000090"
     CDS             7032..7853
                     /locus_tag="VXD58_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0674857.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFIFFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7864..8409
                     /gene="pgsA"
                     /locus_tag="VXD58_000091"
     CDS             7864..8409
                     /gene="pgsA"
                     /locus_tag="VXD58_000091"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV0674858.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8406..9233
                     /locus_tag="VXD58_000092"
     CDS             8406..9233
                     /locus_tag="VXD58_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0674859.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9218..10057
                     /locus_tag="VXD58_000093"
     CDS             9218..10057
                     /locus_tag="VXD58_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV0674860.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10050..10844
                     /locus_tag="VXD58_000094"
     CDS             10050..10844
                     /locus_tag="VXD58_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0674861.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10903..11721
                     /gene="mreC"
                     /locus_tag="VXD58_000095"
     CDS             10903..11721
                     /gene="mreC"
                     /locus_tag="VXD58_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV0674862.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11721..12215
                     /gene="mreD"
                     /locus_tag="VXD58_000096"
     CDS             11721..12215
                     /gene="mreD"
                     /locus_tag="VXD58_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV0674863.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12309..13487
                     /locus_tag="VXD58_000097"
     CDS             12309..13487
                     /locus_tag="VXD58_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV0674864.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13711..14490
                     /gene="rpsB"
                     /locus_tag="VXD58_000098"
     CDS             13711..14490
                     /gene="rpsB"
                     /locus_tag="VXD58_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV0674865.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14569..15609
                     /locus_tag="VXD58_000099"
     CDS             14569..15609
                     /locus_tag="VXD58_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV0674866.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15855..16178
                     /gene="tnpB"
                     /locus_tag="VXD58_000100"
                     /pseudo
     CDS             15855..16178
                     /gene="tnpB"
                     /locus_tag="VXD58_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(16333..16620)
                     /locus_tag="VXD58_000101"
                     /pseudo
     CDS             complement(16333..16620)
                     /locus_tag="VXD58_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16611..17009
                     /locus_tag="VXD58_000102"
     CDS             16611..17009
                     /locus_tag="VXD58_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0674867.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17206..18126)
                     /gene="cysK"
                     /locus_tag="VXD58_000103"
     CDS             complement(17206..18126)
                     /gene="cysK"
                     /locus_tag="VXD58_000103"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV0674868.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYNGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18411..19046)
                     /locus_tag="VXD58_000104"
     CDS             complement(18411..19046)
                     /locus_tag="VXD58_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV0674869.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDNGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19102..20400
                     /locus_tag="VXD58_000105"
     CDS             19102..20400
                     /locus_tag="VXD58_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0674870.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFSNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20397..21059
                     /locus_tag="VXD58_000106"
     CDS             20397..21059
                     /locus_tag="VXD58_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV0674871.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREEGASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21139..21687
                     /gene="raiA"
                     /locus_tag="VXD58_000107"
     CDS             21139..21687
                     /gene="raiA"
                     /locus_tag="VXD58_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV0674872.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21823..23796
                     /locus_tag="VXD58_000108"
     CDS             21823..23796
                     /locus_tag="VXD58_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV0674873.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23793..24245
                     /gene="rplI"
                     /locus_tag="VXD58_000109"
     CDS             23793..24245
                     /gene="rplI"
                     /locus_tag="VXD58_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV0674874.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24289..25641
                     /gene="dnaB"
                     /locus_tag="VXD58_000110"
     CDS             24289..25641
                     /gene="dnaB"
                     /locus_tag="VXD58_000110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV0674875.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25643..25909
                     /locus_tag="VXD58_000111"
     CDS             25643..25909
                     /locus_tag="VXD58_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674876.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25998..27116
                     /gene="cbpD"
                     /locus_tag="VXD58_000112"
     CDS             25998..27116
                     /gene="cbpD"
                     /locus_tag="VXD58_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV0674877.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGLWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27199..27301
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27372..27662
                     /locus_tag="VXD58_000113"
     CDS             27372..27662
                     /locus_tag="VXD58_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV0674878.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27625..28383
                     /locus_tag="VXD58_000114"
     CDS             27625..28383
                     /locus_tag="VXD58_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0674879.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            28421..29428
                     /locus_tag="VXD58_000115"
     CDS             28421..29428
                     /locus_tag="VXD58_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0674880.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29428..30156
                     /locus_tag="VXD58_000116"
     CDS             29428..30156
                     /locus_tag="VXD58_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0674881.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30275..30733
                     /locus_tag="VXD58_000117"
     CDS             30275..30733
                     /locus_tag="VXD58_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV0674882.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30735..33167
                     /locus_tag="VXD58_000118"
     CDS             30735..33167
                     /locus_tag="VXD58_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0674883.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            33488..34141
                     /gene="rr06"
                     /locus_tag="VXD58_000119"
     CDS             33488..34141
                     /gene="rr06"
                     /locus_tag="VXD58_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV0674884.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34138..35478
                     /gene="hk06"
                     /locus_tag="VXD58_000120"
     CDS             34138..35478
                     /gene="hk06"
                     /locus_tag="VXD58_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV0674885.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSRLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35561..36088
                     /locus_tag="VXD58_000121"
     CDS             35561..36088
                     /locus_tag="VXD58_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV0674886.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36167..38083
                     /gene="pspC"
                     /locus_tag="VXD58_000122"
     CDS             36167..38083
                     /gene="pspC"
                     /locus_tag="VXD58_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV0674887.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSEN
                     NPTVTSSGQDISKKYADEVKSHLEKILSEIQTNLDRSKHIKTVNLINKLQDIKRTYLY
                     ELNVLEDKSKAELPSKIKAELDAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKAKAQ
                     KEEDYRNYPTITYKTLELEIAESDVKVKEAELELVKKEADESRNEGTINQAKAKVESE
                     QAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEPATPDKKENDA
                     KSSDSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKDQKEEDHRNYPTITYKTLEL
                     EIAESDVEVKKAELELVKEEAKGSRNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAE
                     EAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAEKPADQQAEEDY
                     ARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQYNGS
                     WYYLNANGDMATGWFQYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAMVTGWLQYN
                     GSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTT
                     VDSYRVNANGEWVN"
     gene            complement(38313..39293)
                     /gene="dusB"
                     /locus_tag="VXD58_000123"
     CDS             complement(38313..39293)
                     /gene="dusB"
                     /locus_tag="VXD58_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV0674888.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(39280..40152)
                     /gene="hslO"
                     /locus_tag="VXD58_000124"
     CDS             complement(39280..40152)
                     /gene="hslO"
                     /locus_tag="VXD58_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV0674889.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            40317..41744
                     /locus_tag="VXD58_000125"
     CDS             40317..41744
                     /locus_tag="VXD58_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0674890.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            41903..43411
                     /gene="glpK"
                     /locus_tag="VXD58_000126"
     CDS             41903..43411
                     /gene="glpK"
                     /locus_tag="VXD58_000126"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV0674891.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            43453..45279
                     /gene="glpO"
                     /locus_tag="VXD58_000127"
     CDS             43453..45279
                     /gene="glpO"
                     /locus_tag="VXD58_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV0674892.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            45349..46053
                     /locus_tag="VXD58_000128"
     CDS             45349..46053
                     /locus_tag="VXD58_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0674893.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            46350..46553
                     /locus_tag="VXD58_000129"
     CDS             46350..46553
                     /locus_tag="VXD58_000129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674894.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(46640..47248)
                     /locus_tag="VXD58_000130"
     CDS             complement(46640..47248)
                     /locus_tag="VXD58_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674895.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            48382..49659
                     /gene="ltrA"
                     /locus_tag="VXD58_000131"
     CDS             48382..49659
                     /gene="ltrA"
                     /locus_tag="VXD58_000131"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV0674896.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGISTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVVSEAAAKRMMYSVSRFIEKRLGLKANMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            50115..51293
                     /locus_tag="VXD58_000132"
     CDS             50115..51293
                     /locus_tag="VXD58_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0674897.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            51607..51738
                     /locus_tag="VXD58_000133"
     CDS             51607..51738
                     /locus_tag="VXD58_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV0674898.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            51759..53309
                     /gene="dltA"
                     /locus_tag="VXD58_000134"
     CDS             51759..53309
                     /gene="dltA"
                     /locus_tag="VXD58_000134"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV0674899.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNAHIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            53306..54550
                     /gene="dltB"
                     /locus_tag="VXD58_000135"
     CDS             53306..54550
                     /gene="dltB"
                     /locus_tag="VXD58_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV0674900.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            54564..54803
                     /gene="dltC"
                     /locus_tag="VXD58_000136"
     CDS             54564..54803
                     /gene="dltC"
                     /locus_tag="VXD58_000136"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV0674901.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            54796..56064
                     /gene="dltD"
                     /locus_tag="VXD58_000137"
     CDS             54796..56064
                     /gene="dltD"
                     /locus_tag="VXD58_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV0674902.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLQQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            56219..56659
                     /gene="adcR"
                     /locus_tag="VXD58_000138"
     CDS             56219..56659
                     /gene="adcR"
                     /locus_tag="VXD58_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV0674903.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            56659..57363
                     /locus_tag="VXD58_000139"
     CDS             56659..57363
                     /locus_tag="VXD58_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0674904.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            57356..58162
                     /locus_tag="VXD58_000140"
     CDS             57356..58162
                     /locus_tag="VXD58_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0674905.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAVVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            58172..59677
                     /gene="adcA"
                     /locus_tag="VXD58_000141"
     CDS             58172..59677
                     /gene="adcA"
                     /locus_tag="VXD58_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV0674906.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYTEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(60848..61621)
                     /locus_tag="VXD58_000142"
     CDS             complement(60848..61621)
                     /locus_tag="VXD58_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0674907.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            61797..63200
                     /locus_tag="VXD58_000143"
     CDS             61797..63200
                     /locus_tag="VXD58_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV0674908.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            63315..63953
                     /locus_tag="VXD58_000144"
     CDS             63315..63953
                     /locus_tag="VXD58_000144"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV0674909.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNTAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            63965..64405
                     /locus_tag="VXD58_000145"
     CDS             63965..64405
                     /locus_tag="VXD58_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV0674910.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            64398..64829
                     /locus_tag="VXD58_000146"
     CDS             64398..64829
                     /locus_tag="VXD58_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0674911.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            64820..>65211
                     /locus_tag="VXD58_000147"
     CDS             64820..>65211
                     /locus_tag="VXD58_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0674912.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFK"
BASE COUNT        20098 a        11159 c        14342 g        19612 t
ORIGIN      
        1 gtaaattata tttacagtat cctttacaca aatctgtaaa aagcaaggct gatttatttt
       61 gataaattac ggttatttca ttaaaaaaat gctataattg aaaggactat atcgaaggag
      121 aacaaaatga ctaaacccat tattttaaca ggagaccgtc caacaggaaa attgcatatt
      181 ggacattatg ttggaagtct caaaaatcga gtattattac aggaagagga taagtatgat
      241 atgtttgtgt tcttggctga ccaacaagcc ttgacagatc atgccaaaga tcctcaaacc
      301 attgtagagt ctatcggaaa tgtggctttg gattatcttg cagttggatt ggatccaaat
      361 aagtcaacta tttttattca aagccagatt ccagagttgg ctgagttgtc tatgtattat
      421 atgaatctag tttcgttagc acgtttggag cgaaatccaa cagtcaagac agagatttct
      481 cagaaaggat ttggagaaag cattccgaca ggattcttgg tctatccaat cgctcaagca
      541 gctgatatca cagctttcaa ggctaattat gttcctgttg ggacagatca gaaaccaatg
      601 attgagcaaa ctcgtgaaat tgttcgttct tttaacaatg catataactg tgatgtcttg
      661 gtagagccgg aaggtattta tccagaaaat gagagagcag ggcgtttgcc tggtttagat
      721 ggaaatgcta aaatgtctaa atcactaaat aatggtattt atttagctga tgatgcggat
      781 actttgcgta aaaaagtaat gagtatgtat acagatccag atcatatccg cgttgaggat
      841 ccaggtaaga ttgagggaaa tatggttttc cattatctag atgtttttgg tcgtccagaa
      901 gatgctcaag aaattgctga tatgaaagaa cgttatcaac gaggtggtct tggtgatgtg
      961 aagaccaagc gttatctact tgaaatatta gaacgtgaac tgggtcctat tcgtgagcgc
     1021 cgtattgaat ttgctaagga tatgggagaa gtttataata tgattcaaaa aggtagtgaa
     1081 agagcgcgtg aagttgcggg tcaaacccta tctgaggtaa aaggggcaat gggacttcat
     1141 tactttaact aagtttattt tacaagaaac tatcttttct atctcgaaga aaagatagtt
     1201 ttttatttac ccctgtaaca tttgacaaat ttttatagaa tggtatgata gatacaatat
     1261 aaaaagagtc aagctcaaaa agaaagaaaa gaggaaactt cgaatgtcta attgggacac
     1321 taaatttttg aaaaaaggtt ttacctttga tgatgtattg cttattccag ctgaaagtca
     1381 tgtgttgcct aacgatgcag atttaacaac taaattggca gataatttga ctttaaatat
     1441 cccaattatt accgctgcca tggacacagt tacagagagt caaatggcca ttgctattgc
     1501 tcgtgcaggc ggtctcggag ttatccataa aaacatgtca attgctcaac aagcagacga
     1561 ggttcgtaag gtaaaacgtt ctgaaaatgg agttattatt gatccgttct tcttgacgcc
     1621 tgaacataca attgctgaag cagatgagct tatgggtcgt taccgcatca gtggtgttcc
     1681 agttgttgaa acacttgaaa atcgtaaatt ggttggtatt ttgacaaacc gagatcttcg
     1741 ttttatttca gattataatc aaccaatttc aaaccatatg actagtgaaa atcttgttac
     1801 tgctcctgtg ggtacggatc ttgcaacggc tgagagtatt cttcaagagc atcgtattga
     1861 aaaacttccg ttggtcgatg aagaaggcag tctttctggt ttgatcacta tcaaagatat
     1921 tgaaaaagtt attgagtttc caaatgcggc taaagatgag tttggtcgtc ttctagttgc
     1981 aggtgcagta ggtgttactt cagatacatt tgaacgtgca gaggctcttt ttgaggcagg
     2041 agcggatgcg attgttattg atactgcaca tggtcattct gcaggtgtct tgcgtaaaat
     2101 tgccgagatt cgtgctcatt tcccagatcg gactttgatt gctggaaata ttgctactgc
     2161 tgaaggtgca cgtgcccttt atgaagcggg tgtagacgtt gttaaggttg gtattggacc
     2221 aggttctatc tgtactactc gtgtgattgc tggtgttggt gttccgcaag taacagctat
     2281 ctacgatgct gcagctgttg cgcgcgaata tggtaaaacg attattgctg acggtgggat
     2341 caagtattct ggagatattg taaaagcact tgctgcaggt ggaaatgctg ttatgcttgg
     2401 atctatgttt gctggaactg atgaagctcc aggcgaaact gaaatcttcc aaggacgtaa
     2461 attcaagact taccgtggta tgggatcaat tgctgctatg aagaaaggtt caagcgaccg
     2521 ttatttccaa ggttctgtca atgaagcaaa caagcttgtt ccagaaggaa ttgaaggtcg
     2581 tgttgcttat aaaggagcgg cagctgatat tgttttccaa atgattggtg gtattcgctc
     2641 tggtatgggt tactgtggtg cagctaacct taaagaacta cacgataatg ctcaatttat
     2701 tgaaatgtct ggtgctggtt tgaaagaaag ccatcctcat gatgtgcaaa ttactaatga
     2761 ggcaccaaat tattctatgt aaaaaacaat gaaaagaact ccagtgaaaa caggagttct
     2821 tttacaatgt tgtcaatttc catttacaga agctttacca tcctgaatag tgaagatact
     2881 tagattttct ggcagatttt gaagatggtc taagcttgtt gttgtgataa aggtttggat
     2941 tgattgagaa atcgtttcta ataattttaa ctgtctagtg ttgtcaagtt cactcatcac
     3001 atcgtcaagc agtaatatcg gagattctgt ggtaatgctt tccattaatt cgatttctgc
     3061 taattttatc gagaggacga gactacgatg ttgaccttgg cttccgaaac tagcatccat
     3121 cccatttata taaaaagaaa tgtcatctcg atgaggaccg acaccagtat tctttttaaa
     3181 taaatctctg gatctacttt tttctaaagc aattttgaaa gattcggata agttttgttt
     3241 gtcagttata ttgacagaag attgatagga tattgacaac tcttcgatct gattagagag
     3301 ttcaaaatgt ttcttacgcc caaatgattc tagttttttt atgaaatcta agcggtgatt
     3361 cattacacga catccataat caactagctg atcatctaac acagaaagga atgtttcatc
     3421 tattttttga tctgatttta ggtaagtgtt tctttgcttt aggatgtggt tataattggt
     3481 taagtcagat aagtagattg gcttaatttg cccaagttcc atatcaatga attttcgtcg
     3541 aatcgaaggt gctcctttaa ttagttgtaa atcttcagga gcaaataaga caacattcat
     3601 gtgtcctaca taatctgaaa ggcgtgcctg ttttaagtga ttaacttttg ttacacggcc
     3661 tttttgtgtt agttcgattt ctaggggaat ggatccagtt tttttctgaa cgagacctga
     3721 aagatgaagt tgttcctcat caaaatgaat gagattttta tctgttcgag ttcgatgact
     3781 acgcgttaag gctaaaaaat agatagcctc taacatgttt gtttttcctt gtgcattacg
     3841 tcccaaaaag acattcaatt taggattaaa gtctattttc gtttctttgt aattacgaaa
     3901 agtcttgaga gatagatgtt gtagccacat gattatctac cagggaatcg tggagcttgt
     3961 tggcttttag gtgatgaagc aggtttcgat ttgtcttttt taactccctt attcatcttt
     4021 ttgacaagtt tagcgatccg ttctttttca actttatcag cttggtattc atcttgttct
     4081 tcagaagtag gttgtgtcaa caagatgtca atgttcatgt cagggatgtc aactttatcg
     4141 ccaatacgaa gtttttttcc acgacgactc tctaattccc tattaaagta aacagaatgt
     4201 tcagagagaa atgatttaat agctcctccg ctatgtgtaa ttccaagttc tttgagtagt
     4261 gcttggaggg taataaattc ttcaaataat ttgtattcca taattcacct caatctttgg
     4321 tattatacca tattttccta aaaatagtga gagagacagc gagaaatgta agcgattttt
     4381 atttcaaaag tgttacaggg aacaagaaaa tttcaggttt tcgtgatata atagaagtct
     4441 gtatataagg aggtaaatca tggagttagt gcatggaatt tcaacacatt ttatccaatc
     4501 aaaaaagttt aaaacaaaca aaattaccgt gcgttttacc gctccattat cccttgatac
     4561 gattgcaggt cacatgttga gtgcaagtat gctagagact gctaatcaga tgtaccccac
     4621 ttctcaagat ttgaggagac acttggccag tctatacggt acagatatgt caaccaattg
     4681 tttcagaaga gggcaaagcc acattataga attgacattt acctatgttc gtgatgagtt
     4741 tttaagtagg aaaaacgtgc taacctctca gattttggaa cttgtaaaag aaactctttt
     4801 ttcacccgta gtagttgata atgggtttga tccggcctta tttgaaattg agaaaaaaca
     4861 attgctagca agtttagcag ctgatatgga tgattctttt tattttgcac ataaagaatt
     4921 ggataaattg ttttttcatg atgaacgtct tcaattggaa tatagtgatt tacgaaatcg
     4981 tattttagct gaaactccac aaagttctta ttcttgtttc caagaatttt tagccaatga
     5041 tcgaatagat ttctttttcc taggtgattt taatgaggtt gaaattcaaa atgtattaga
     5101 atcatttggc tttaaaggtc gaaaaggaga tgtgaaggtt cagtattgtc aaccttattc
     5161 taatatcctt caggaaggta tggttcggaa aaatgtggga caatccattt tggaattagg
     5221 ttatcattac tgttctaaat atggtgatga gcaacattta cccatgattg taatgaatgg
     5281 tttacttggt ggatttgctc actctaagct ctttacaaat gtccgtgaaa atgctggatt
     5341 agcttatacc atttcaagtg agcttgattt atttagtgga ttcttgagga tgtatgctgg
     5401 tatcaatcga gaaaatcgta accaggctcg taaaatgatg aataatcaac tgcttgattt
     5461 aaaaaaaggt tattttacag agtttgagtt aaatcagacc aaggaaatga ttcgttggtc
     5521 gttgttactt tctcaagata atcaatcttc attgattgaa cgtgcttatc aaaatgcctt
     5581 atttggaaaa tcttcagcag actttaaaag ttggattgca aagcttgaac agattgacaa
     5641 agatgctatt tgtagagtag ctaataatgt gaaactacaa gcgatttact ttatggaagg
     5701 aatagaatga caaaggttgt ttttgaagaa aaatactatc cagctgtaaa agaaaaggtt
     5761 tatcgaactc gtttggccaa cggattgaca gttgctcttt tgcctaaaaa ggaatttaaa
     5821 gaggtttacg ggagtgtaac tgtacagttt ggttcgatag atacgcttgt cacagaagtt
     5881 gacggagatg taaaacaata tcctggagga attgctcatt ttcttgaaca taaattattt
     5941 gagagagaag attctagtga tttgatgtcg gcttttacga gtctaggtgc agatagtaat
     6001 gcctttacaa gctttacaaa aacaaactat cttttttcag caacggatta ttttttagaa
     6061 aatttatatt tacttgatga attggtaaca tcagcacact ttactgaagc ttccattctg
     6121 acagagcagg atattattca gcaagaacga gaaatgtacc aagatgatcc agattcgtgt
     6181 ttattctttt caactttagc gaatttgtat cctggtacac ctttagcaac tgatatagtt
     6241 ggaagtgagg agtccatttc ccaaatcaat ctaactaatt tgcaagaaaa ttttacaaag
     6301 ttttacaaac ctgtaaacat gtctctgttt ttagttggta attttgatgt ggagcgagta
     6361 caggactatt ttgaaagcaa agaactgaaa gattcagatt ttcaggaagt agcaagagaa
     6421 aagttgtttt tacagcctgt aaagccaaca gatagtatga gaatggaagt atcttctccc
     6481 aaactagcga ttggagttag aggtaagcga gaagtttctg aagcggattg ctatcgacat
     6541 catattttat taaaattatt gtttgcaatg atgtttggtt ggacttcgga tcgttttcaa
     6601 aaatgttatg aatcaggtaa aattgatgcg tccttatctc tggaagttga aataacaagt
     6661 cgctttcatt ttgtcatgtt gacaatggat acgaaagagc cagttgcttt gtctcatcaa
     6721 tttaggaagg ctattcgtaa ttttacaaag gatttagata ttacagagga acatttagat
     6781 attatcaaaa gagagatgtt tggcgaattt ttcagtagca tgaactctct tgaatttatt
     6841 gcaacgcaat atgatgcttt tgaaaatggt gagacaattt ttgatttgcc gaaaatttta
     6901 caggaaatta ctttagagga tgtccttgat gctggacatc atttaataga tgatggtgac
     6961 atagttgatt ttacaatatt cccatcgtag taacctatta taatagacac tagaaagaag
     7021 ggatgacaag tatgagaaaa aaaacaattg gagaggtttt acgattagct agaatcaatc
     7081 agggattgag tttagatgaa ttgcagaaaa agacagaaat ccagttagat atgttggaag
     7141 caatggaagc agacgatttc gatcaacttc caagtccttt ttacacgcgt tctttcttga
     7201 aaaaatatgc atgggctgtt gagttagatg accaaattgt tttggatgct tatgattctg
     7261 ggagtatgat tacttatgag gaagtagatg ttgatgaaga tgagttgaca ggtcgtagac
     7321 gttcaagtaa gaaaaagaag aaaaaaatat catttttacc tttattttat tttatctttt
     7381 ttgctttatc gattttaatt tttgtgactt attatgtttg gaactatatt caaactcaac
     7441 cagaggagcc ttctctttct aattacagtg tggttcaatc aacaagttca actagctctg
     7501 ttccccactc ctcaagtagt agttcttcta gtatagaatc agctataagt gtatcaggcg
     7561 aaggaaatca tgtagaaatc gcttataaga caagtaagga aacagttaaa ttgcaattgg
     7621 cagtttcaga tgttacaagt tgggtcagtg tttcagaaag cgaacttgag ggcggtgtaa
     7681 ccttatcgcc aaagaagaaa agtgcagaag caacagttgc aactaaaagt cctgtaacaa
     7741 ttacgttagg tgttgtaaaa ggtgttgatt tgacagtaga taatcagact gttgatttat
     7801 cgaaattaac agctcagact ggacaaatca ctgtaacctt tactaaaaat taaggaaaaa
     7861 cgaatgaaaa aagaacaaat tcccaatctc ttaacaatag gtcgaattct ctttatacct
     7921 atttttatct ttattttaac gataggaaat tcgatagaga gtcatatagt tgcagctatt
     7981 atctttgctg ttgccagtat taccgactat ttagatggat atttagctcg taaatggaat
     8041 gtggtcagta attttggtaa atttgcagat cctatggcgg ataagttact agttatgtcg
     8101 gcttttatta tgttgattga gttaggtatg gctccggctt ggattgttgc agtgattatc
     8161 tgtcgtgagt tagctgtgac aggtttaagg cttttattgg ttgaaactgg tggaacaatt
     8221 ttagcagcag caatgcctgg aaaaattaaa acttttagtc agatgtttgc cattattttc
     8281 ttgctattac attggacttt gcttggtcaa gttctacttt atgtagcctt atttttcact
     8341 atctactctg gctatgacta tttcaagggt agtgcctatg tatttaaagg gacatttggt
     8401 tcgaaatgaa atcaataatt gatgtaaaaa atctttcttt tcgctataaa gaaaatcaga
     8461 actactacga tgtgaaggat attacgtttc acgtgaaacg tggagaatgg ctttcgattg
     8521 tagggcataa tggtagtggt aaatcaacga cggttcgatt aattgatggc ttactggaag
     8581 cagaatccgg agagattgta attgatggcc aacgtttgac tgaggaaaat gtttggaata
     8641 tacgtcgtca aatcggtatg gtttttcaaa atccagacaa tcaatttgtt ggagcgactg
     8701 ttgaagatga tgttgctttt ggtttggaaa atcagggact ttctcgtcaa gaaatgaaaa
     8761 agagagtgga agaagctctg gctttagttg gcatgttgga ctttaaaaag agagagccag
     8821 cgcgtctatc aggtggccaa aagcaacgtg tggccattgc aggtgttgta gccctaagac
     8881 cagctatttt aatcttagat gaagcaacga gtatgttgga tcctgagggg cgtagagaac
     8941 ttattgggac agtaaaagga attcgaaaag actatgatat gacggtcatt tctattaccc
     9001 atgatttgga agaagtcgcc atgagtgatc gcgtattggt catgaaaaaa ggggaaattg
     9061 aatcaactag tagtccaagg gagcttttct ctcgaaatga tttagatcaa attggattag
     9121 acgatccttt tgctaatcaa ttaaaaaaat ctttgagcca gaatggctat gatttacctg
     9181 aaaattattt gacagaaagt gagctagagg ataagctatg ggaattgctc tagaaaatgt
     9241 gaattttaca tatcaagaag gtactccctt agcttcagca gctttgtcgg atgtttcttt
     9301 gacgattgaa gatggctctt atacagcttt aattgggcac acaggtagtg gtaaatcaac
     9361 tattttacaa ctcttaaatg gtttattggt gccaagtcaa gggagtgtga gggtttttga
     9421 taccttaatc acctcgactt ctaaaaataa agatattcgt caaattagaa aacaggttgg
     9481 cttggtattt cagtttgctg aaaatcagat ttttgaagaa acggttttga aggacgttgc
     9541 ttttggaccg caaaattttg gagtttctga agaagatgct gtgaagactg cgcgtgagaa
     9601 actggctctg gttggaattg atgaatcact ttttgatcgt agtccgtttg agctgtcagg
     9661 gggacaaatg agacgtgttg ccattgcagg catacttgcc atggagccag ctatattagt
     9721 cttagatgag ccaacagctg gtctagatcc tctagggaga aaagagttga tgaccctgtt
     9781 caaaaaactc caccagtcag ggatgaccat cgtcttggta acgcatttga tggatgatgt
     9841 tgctgaatat gcgaatcaag tctatgtaat ggaaaaggga cgtttagtaa aggggggcaa
     9901 accaagtgat gtctttcaag acgttgtttt tatggaagaa gttcagttgg gagtacctaa
     9961 aattacggcc ttttgtaaac gattggctga tagaggcgtg tcatttaaac gattaccgat
    10021 taagatagag gagttcaagg agtcgctaaa tggatagtat gattttaggg cgttatatcc
    10081 caggggattc gattgttcac cgattggatc cacgtagcaa attgcttgct atgatgctac
    10141 tgattttgat tgtgttttgg gctaataacc ccttgacgaa tctgattctt tttatagcga
    10201 cagggatatt tattgccttg tcaggagtat ctctttcatt ttttattcag ggcttgaagt
    10261 ctatgttttt cttgattgcc ttcacaacta tttttcaact attttttatt tctaatggga
    10321 atgttttatt tgagttttcg tttgtgagaa tcacggatta tgctttgcaa caagctggga
    10381 ttattttttg tcgctttgta ttgattattt tcttttcaac tttgttaacc ttaacaacca
    10441 tgcctttaag tttggcatca gctgtcgaag ctttattagc acctttaaag cgtgtgaaag
    10501 ttccagttca tgaaattgga ttgatgctgt ctatgagttt gcgttttgtc ccaaccttga
    10561 tggatgatac gactcggatt atgaatgcac agaaagctcg tggagtggat tttggagaag
    10621 gaagcatcgt tcaaaaagta aaggcgatga ttcccatttt gattcctctt tttgcgacaa
    10681 gtttaaaacg tgcagattcc ttggctatcg ctatggaagc gcgtggctat cagggtggaa
    10741 aaggcagaag tcaataccga caattgaaat ggactctgaa ggatacgctg accattcttg
    10801 ttattctcgt acttggttgt tgtttatttt tcttaaaatc ttagtagctt tcaggaattg
    10861 ataaaaagtt actgtaacag tttttgatat aaaggtagga atatgaaccg ttttaaaaaa
    10921 tcaaaatatg tcattattgt ttttgtcact gttctgcttg tgtcagctct cttagcgacg
    10981 acttattcaa gtacaattgt gacaaaatta ggagatggaa tctcattggt tgatagagtt
    11041 gtacaaaaac cttttcagtg gtttgattct gtcaaatcag atttggctca tttgacacga
    11101 acatataatg aaaatgaaag tttgaagaaa cagctttacc aattagaagt taaatcaaat
    11161 gaggtggaaa gtttaaagac agaaaatgaa caactgcgcc aattgcttga tatgaagtct
    11221 aaattgcaag ccacaaagac tttagcagca gatgttatta tgcgttctcc ggtatcttgg
    11281 aagcaggagt tgaccttaga tgcaggtaga tcaaaaggtg cttctgagaa catgttagct
    11341 attgcaaatg gtggcttgat tgggagtgtt tcaaaagtag aggagaactc tactatagtc
    11401 aaccttctga caaatacgga aaatgctgat aagatttctg ttaaaattca acatggctct
    11461 actacaattt atggaattat tattggctat gacaaggaaa atgacgttct taaaattagc
    11521 caattaaata gtaatagcga tattagtgcg ggagataagg tgactactgg tggattagga
    11581 aactttaacg ttgctgatat tcctgttggt gaagtggttg ccacaacgca tagtacagac
    11641 tatttgacac gagaagtaac tgttaaattg agtgcagata ctcataatgt agatgtgata
    11701 gaattagtgg ggaattcata atgagacagt tgaagcgagt tggagtattt ttattgcttc
    11761 ctttctttgt tctaattgac gcccatatta gccagcttct gggctcattt ttcccccatg
    11821 tacatttggc tagtcatttt ctttttctat ttctcttatt tgagacgata gaagtatcag
    11881 agtatctcta cctagtctat tgttttgtta taggcttggt ttatgatgtt tactttttcc
    11941 atctaatagg gattacaact ctcttattta tcttattggg agccttcctt cataaattga
    12001 atagtgttat tttgttgaat cgttggacaa gaatgctagc tatgattgtg ctgacattcc
    12061 tgtttgaaat gggtagttat cttttggctt ttatggtagg gttgacagta gatagcatgt
    12121 cgatttttat agtctatagc ttggtaccga cgatgatttt aaatttttta tggattactg
    12181 tttttcaatt tatttttgaa aaatattatc tataagaacg acatataaat gtaacaaagg
    12241 cgtaatattt attaggcctt tttttggtat actagtattg tctttaaaag aaggagtatc
    12301 tacgtaatat gaagaaaaaa atcttagcgt cacttttatt aagtacagta atggtttctc
    12361 aagtagctgt tttaacaact gcgcatgcag aaacgactga tgacaaaatt gctgctcaag
    12421 ataataaaat tagtaactta acagcacaac aacaagaagc ccaaaaacaa gttgaccaaa
    12481 ttcaggagca agtatcagct attcaagctg agcagtctaa cttgcaagct gaaaatgata
    12541 gattacaagc agaatctaag aaactcgagg gtgagattac agaactttct aaaaacattg
    12601 tttctcgtaa ccaatcgttg gaaaaacaag ctcgtagtgc tcaaacaaat ggagccgtaa
    12661 ctagctatat caataccatt gtaaactcaa aatcaattac agaagctatt tcacgtgttg
    12721 ctgcaatgag tgaaatcgta tctgcaaaca acaaaatgtt agaacaacaa aaggcagata
    12781 aaaaagctat ttctgaaaaa caagtagcaa ataatgatgc tatcaatact gtaattgcta
    12841 atcaacaaaa attggctgat gatgctcaag cattgactac gaaacaggca gaactaaaag
    12901 ctgctgaatt aagtcttgct gctgagaaag cgacagctga aggggaaaaa gcaagtctat
    12961 tagagcaaaa agcagcagct gaggcagagg ctcgtgcagc tgcggtagca gaagcagctt
    13021 ataaagaaaa acgagctagc caacaacaat cagtacttgc ttcagcaaac actaacttaa
    13081 cagctcaagt gcaagcagta tctgaatctg cagcagcacc tgtccgtgca aaagttcgtc
    13141 caacatacag tacaaacgct tcaagttatc caattggaga atgtacatgg ggagtaaaaa
    13201 cattggcacc ttgggctgga gactactggg gtaatggagc acagtgggct acaagtgcag
    13261 cagcagcagg tttccgtaca ggttcaacac ctcaagttgg agcaattgca tgttggaatg
    13321 atggtggata tggtcacgta gcggttgtta cagctgttga atcaacaaca cgtatccaag
    13381 tatcagaatc aaattatgca ggtaatcgta caattggaaa tcaccgtgga tggttcaatc
    13441 caacaacaac ttctgaaggt tttgttacat atatttatgc agattaattt acagagggac
    13501 tcgaatagag ccctcttttc aggttttacc gtgacaatcc ctattaaaaa ttatatcaaa
    13561 atagcttgaa aatattggaa aagtatggta gaatgaaaat tgtcgtgtga acgataatac
    13621 tcattcttga tgaattgtga agcagttgcc cttgggtcgt tttgcgagtt gaagtcaaga
    13681 agaggaaaaa aacaaaaagg agaaatactc atggcagtaa tttcaatgaa acaacttctt
    13741 gaggctggtg tacactttgg tcaccaaact cgtcgctgga atcctaagat ggctaagtac
    13801 atctttactg aacgtaacgg aatccacgtt atcgacttgc aacaaactgt aaaatacgct
    13861 gaccaagcat acgacttcat gcgtgatgca gcagctaacg atgcagttgt attgttcgtt
    13921 ggtactaaga aacaagcagc tgatgcagtt gctgaagaag cagtacgttc aggtcaatac
    13981 ttcatcaacc accgttggtt gggtggaact cttacaaact ggggaacaat ccaaaaacgt
    14041 atcgctcgtt tgaaagaaat taaacgtatg gaagaagatg gaactttcga agttcttcct
    14101 aagaaagaag ttgcacttct taacaaacaa cgtgcgcgtc ttgaaaaatt cttgggcggt
    14161 atcgaagata tgcctcgtat cccagatgtg atgtacgtag ttgacccaca taaagagcaa
    14221 atcgctgtta aagaagctaa aaaattggga atcccagttg tagcgatggt tgacaccaat
    14281 actgatccag atgatatcga tgtaatcatc ccagctaacg atgacgctat ccgtgctgtt
    14341 aaattgatca cagctaaatt ggctgacgct attatcgaag gacgtcaagg tgaggatgca
    14401 gtagcagttg aagcagaatt tgcagcttca gaaactcaag cagattcaat tgaagaaatc
    14461 gttgaagttg tagaaggcga caacgcttaa tttatacaaa tagtaattac ctaggagggc
    14521 ggggcttagc ccggctctcc tattttcaaa aaatatagga gaattaaaat ggcagaaatt
    14581 acagctaaac ttgtaaaaga gttgcgtgaa aaatctggtg ccggtgttat ggacgctaaa
    14641 aaagcgcttg tagaaacaga cggtgacatc gaaaaagcga ttgaattgct tcgtgaaaaa
    14701 ggtatggcta aggcagctaa gaaagctgac cgtgttgctg cagaaggttt gactggtgtt
    14761 tatgttaacg gtaatgttgc agcagttatt gaagtaaacg ctgaaactga cttcgttgca
    14821 aaaaacgctc aattcgttga attggtaaat actacagcta aagtcattgc tgaaggaaaa
    14881 cctgctaaca acgaagaagc tcttgctttg ataatgcctt caggtgaaac tcttgaagct
    14941 gcatacgtat ctgcaacagc aactatcgga gagaaaatct cattccgtcg ctttgcattg
    15001 attgaaaaaa cagacgcaca acactttgga gcataccaac ataacggtgg acgtatcggt
    15061 gttatttcag ttgttgaagg tggagacgaa gcacttgcta aacaattgtc aatgcacatc
    15121 gcagcgatga aaccaacagt tctttcttac aaagaattgg atgagcaatt cgttaaagat
    15181 gagttggcac aattgaatca cgttatcgac caagacaacg aaagccgtgc aatggttaat
    15241 aaaccagctc ttccacactt gaagtatgga tcaaaagctc aattaactga tgatgttatt
    15301 gctcaagctg aagctgacat caaagctgaa ttggctgcag aaggtaaacc agaaaaaatc
    15361 tgggacaaaa ttattccagg taaaatggat cgcttcatgc ttgataacac taaagttgac
    15421 caagcttaca cacttcttgc acaagtttac atcatggatg acagcaagac agttgaagca
    15481 taccttgaat cagttaacgc ttcggtagtt gagtttgctc gctttgaagt tggtgaaggt
    15541 atcgagaaag ctgcaaacga cttcgaagct gaagttgcag ctacaatggc agcagccttg
    15601 aataactaat aatagaaagg aagcaaattg cttccttttt tgtatagggc ttagattttt
    15661 cctgaaattg ttttttcccc agcctcactc gattagaata attaaaacaa aaagccctat
    15721 aataagggct aagtgcattt aggagactat acttcaaatt catagtaagc gcgtcataac
    15781 aaggtatcta tcctaattcc ccaacttcca gtatactaga taaaaaataa ttatcggagg
    15841 aaaggtattg tactatgata attcaactaa gtgatttagg tcaagttcac cttgtttgtg
    15901 acaagacaga tatgaggtag ggaatagact cattagccta tgtagttaaa acccactttg
    15961 aattggattc tttctccggt caagtctttc tcttttgtgg tgaacgtaaa gaccgcttta
    16021 aagcccttta ctgggatggt caaggatttt ggctactata taaacgcttt gagaacggaa
    16081 aactgacttg gctaagtaca gaaaaggatg tcaaagctct cgcacctgaa caagtagatt
    16141 ggcttatgaa gggcttttct atcactccaa aaatatagta gattgaaact agaatagtac
    16201 acctctgctt ctaaaacatt gttagaaatc gatttgattg tcctgatcga tttgtcctgt
    16261 tcttatttca ttttactata aatccatcag aaagttgtga tttctattga aatgaggact
    16321 ttctttttac actcatctgc tttcaaaaag cattctagtc catctccgat taacgatgga
    16381 ctttatcacc tccttctcca gtccttgtat gacatcttcc aaagttcgaa aggctttatt
    16441 cttaaatcca cgtttacgaa tctctttcca tacttgttca atggggttca tctctggtgt
    16501 gtatggagga ataaatgcaa agccaatatt agtcggaatc tttaaggtac ttgatttatg
    16561 ccatatagca ttgtccataa cgagtaaaag ataatcatct ggataagctt gtgaaagctc
    16621 ctattcctaa agccccttta gcgcatagct ttggctcagc ttctattatc gctcacacca
    16681 tccatcagaa gtttaatctg aaggtaccca attatcgcca agaagaagat tgggctaaga
    16741 tgggtttacc aatcacacgc aaggaaatct ctaattggca tatcaagacg agtcaatact
    16801 atttggagcc cctctataac ctcttgcgag agagactatt gactcagccc ttacttcatg
    16861 cggatgaaac ttcttatagg gtgctagaga gtgatagtca gctgacttac tattggactt
    16921 ttttgtctgg gaaagctgag aaacaaggga ttacgcttta ccatcatgtc ctaatcgatt
    16981 tgttcatatc ttatttcaat ccattataaa tagtgagaaa tatctatcct atcttctaga
    17041 atgtcttcca aacgtggaaa ctctcgtaaa caaagaggtt ttagaggctt atttaccatg
    17101 gactaaaatt gtacaagaaa agtgcaaata agaaatctcc agattaggaa ctatccgtga
    17161 gttctctagt ctggagattt ttcaatatac ttcgttattg gacggttaca attcataaag
    17221 tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt ttttacctgt
    17281 acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg aaatccctac
    17341 aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat ctgatgttac
    17401 acgaacgata ccattatagg ctttagtatc aagtgtatca ggaataaatc cagctgagat
    17461 accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt catctgcttc
    17521 tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa caccagaaat
    17581 cgttccaccg gtacctactc cagcaacaaa ggcatctaat ccatctttac cgaaagcagc
    17641 tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat tgtcaaattg
    17701 aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa tagcaccttt
    17761 cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga taattttacg
    17821 tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt tagcagcacc
    17881 tacccatgaa agtccaatac cagtgtttcc acttgttgct tcaacaatag tagaaccagg
    17941 tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac ggtcttttac
    18001 agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac cttctggcac
    18061 gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt tgttataaat
    18121 agacatatga atacctcttt gtataagata tctttagtat aacgctatcc ataccatttg
    18181 taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat caaagtcatt
    18241 aattttcaag tgattgcgtt cagctaattc tagtaaatag gtgtgaagga aaacgctatc
    18301 aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt tttattttgc
    18361 tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt ttacactaag
    18421 tttacaggaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt tccattaaaa
    18481 aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata aatcatcgta
    18541 tcgacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt aaggaagtta
    18601 ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg ttcttttatt
    18661 tcaataatac caatttcttt gacagctaag gcgacactgc cggcgtaagc acgaattagt
    18721 cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca gacattggtg
    18781 agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact aggctcacca
    18841 ttatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga gcagttatgt
    18901 gtcgctttgt agtgttcttt tttgatggca gtaatgaagt cacgagcctc ttcttcgcta
    18961 taaacacgct tggcatggca gataaagcga gattttttta tttcttcttg gacttggccg
    19021 tcctctttaa ttgtcctaaa ttccatgatt taattgtact aaaaaataat ctaaagtgct
    19081 agtttttacg aatagagaag tatgaaagta aatttagatt atctcggtcg tttatttact
    19141 gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc aatgagaaag
    19201 gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga atggtatttg
    19261 cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag aagtgatcaa
    19321 actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa atggcgcggc
    19381 caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt agacaagcaa
    19441 aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat ttatcaagta
    19501 gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg catagatgtt
    19561 tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcgggatagc tttgctacat
    19621 ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca tcagttattg
    19681 aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt tccttatgtt
    19741 gataatccca cgctttacca cgctgtcaag aatagtgtaa aggagaatgg attgagaatc
    19801 tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg agaactaaaa
    19861 agactgagtt taccgagacg gtttcatgga aatccgttga ttattccaaa accaatttgg
    19921 ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt aaagtcctat
    19981 attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga aattaagaaa
    20041 ggggagcagt tagaagaaat cttacaggag caattttcaa atgagaaaat tggctttgta
    20101 tcttctgtaa cagaggatcg attagagcaa gtacaagctt ttcgagatgg agaactgaca
    20161 atacttatca gtacgacaat cttggagcgt ggagttacct tcccttgtgt ggatgttttc
    20221 gtagtagagg ccaatcatcg tttgtttacc aagtctaatt tgattcagat tggtggacga
    20281 gttggacgaa gcatggatag accgacagga gatttgcttt tcttccatga tgggttaaat
    20341 gcttcaatca agaaggcgat taaggaaatt cagatgatga ataaggaggc tggtctatga
    20401 agtgcttgtt atgtgggcag actatgaaga ctgttttaac ttttagtagt ctcttacttc
    20461 tgaggaatga tgactcttgt ctttgttcag actgtgattc tacttttgaa agaattgggg
    20521 aagagaactg tccaaattgt atgaaaacag agttgtcaac aaagtgtcaa gattgtcaac
    20581 tttggtgtaa agaaggagtt gaagtcagtc atagagcgat ttttacttac aatcaagcta
    20641 tgaaggattt tttcagtcgg tataagtttg atggagactt cctgttaaga aaagttttcg
    20701 cttcattttt aagtgaggag ttgaaaaagt acaaagagta tcaatttgtt gtaattcccc
    20761 taagtcctga tagatatgct aatagaggat ttaatcaggt tgagggcttg gtagaggcag
    20821 caggctttga gtatctggat ttattagaga aaagagaaga gggagccagt tcttctaaaa
    20881 atcgttcaga gcgcttgggg acagaacttc ctttctttat taaaagtgga gtcactattc
    20941 ctaaaaaaat cctacttata gatgatatct atactacagg agcaactata aatcgtgtta
    21001 agaaactgtt ggaagaagct ggtgctaagg atgtaaaaac attttccctt gtaagatgag
    21061 gaaaaaattt gcaaaaatag aatgaaagcg ttataataaa atcataaaaa caaaaatgtt
    21121 tagaaagaag gtactcatat gattaaatat agtatccgtg gtgaaaacct agaagtaaca
    21181 gaagcaattc gtgattatgt agtttctaaa ctcgaaaaga tcgaaaagta cttccaacca
    21241 gaacaagagt tggatgcccg aattaactta aaagtttatc gtgaaaaaac ggctaaagtg
    21301 gaagtaacga ttccgcttgg atctattact ctccgcgcag aagatgtatc tcaagatatg
    21361 tatggttcaa ttgaccttgt aactgataaa attgaacgtc agattcgtaa aaataaaaca
    21421 aaaatcgagc gtaaaaataa aaataaggta gcaactggtc aattatttac agatgctttg
    21481 gtggaagatt caaatgttgt ccagtctaaa gttgttcgtt caaaacaaat tgatttaaaa
    21541 ccaatggatt tggaagaagc aattctacaa atggatttat tggggcatga tttctttatc
    21601 tatgtggatg ttgaagatca aacaaccaat gtgatttatc gtcgtgagga tggcgaaatt
    21661 ggtttgttag aggttaaaga atcttaattt caatatgtgt aaaggtaggt ttactgaatt
    21721 gtaaactacc tttttcttat aattgataga attactgatt acacttttaa aaagtcgctt
    21781 tttgaggggt attatggtat aatgggtgcc aagaggtttt gaatgaaaaa attttatgta
    21841 agtccaattt ttcctattct agtaggattg attgcgtttg gagtcttatc cactttcatt
    21901 atttttgtta ataataatct gttgacggtt ttaattttgt ttctttttgt aggaggctat
    21961 gtttttttat ttaagaaact gagagtgcat tatacaagga gtgatgtaga acagatacag
    22021 tatgtaaacc accaagcgga agaaagtttg acagctctat tggaacagat gcctgtaggt
    22081 gttatgaaat tgaatttatc ttctggagag gttgagtggt ttaatcccta tgctgaattg
    22141 attttgacca aggaagatgg tgattttgat ttagaagctg ttcaaacgat tatcaaggct
    22201 tcagtaggaa atccgtctac ttatgccaag cttggtgaga agcgttatgc tgttcatatg
    22261 gatgcttctt ccggtgtttt gtattttgta gatgtatcca gggaacaagc cataacagat
    22321 gaattggtaa caagtcgacc agtgattggg attgtctctg tggataatta tgatgatttg
    22381 gaggatgcaa cttctgagtc agatattagt caaatcaata gttttgtagc taattttata
    22441 tcagagtttt cagaaaaata catgatgttt tctcgtcggg taagtatgga tcgattttat
    22501 ctatttactg actacacggt gcttgagggc ttgatgaatg ataaattttc tgttattgat
    22561 gctttcagag aagagtcgaa acagagacag ttgcccttga ccttaagtat gggattttct
    22621 tatggcgatg gaaatcatga tgagataggg aaagttgctt tgctcaattt gaacttggct
    22681 gaagtacgtg gtggcgacca ggtggttgtt aaggaaaacg acgaaacgaa aaatccagtt
    22741 tattttggtg gtgggtctgc tgcttcaatc aagcgtacac ggactcgtac gcgcgctatg
    22801 atgacagcta tttcagataa gattcggagt gtagatcagg tttttgtagt cggtcacaaa
    22861 aatttagaca tggatgcttt gggctctgct gtaggtatgc agttgttcgc cagcaatgtg
    22921 attgaaaata gctatgctct ttatgatgaa gaacaaatgt ctccagatat tgaacgagct
    22981 gtttcattca tagaaaaaga aggagttacg aagttgttgt ctgttaagga tgcaatgggg
    23041 atggtgacca atcgttcttt gttgattctt gtagaccatt caaagacagc cttaacatta
    23101 tcaaaagaat tttatgattt atttacccaa accattgtta ttgaccacca tagaagggat
    23161 caggattttc cagataatgc ggtcattact tatatcgaaa gtggtgcaag tagtgccagt
    23221 gagttggtaa cggaattgat tcagttccag aattctaaga aaaatcgttt gagtcgtatg
    23281 caagcaagtg ttttgatggc tggtatgatg ttggatacta aaaatttcac ctcgcgagta
    23341 actagtcgga catttgatgt tgctagctat ctcagaacgc gcggaagtga tagtattgct
    23401 atccaggaaa tcgctgcgac agattttgaa gaatatcgtg aggtcaatga acttatttta
    23461 caggggcgta aattaggttc agatgtacta atagcagagg ctaaggacac gaaatgctat
    23521 gatacagttg ttattagtaa ggcagcagat gccatgttag ccatgtcagg tattgaagcg
    23581 agttttgttc ttgcgaagaa tacacaagga tttatctcta tctcagctcg aagtcgtagt
    23641 aaactgaatg tacaacggat tatggaagag ttaggcggtg gaggccactt taatttggca
    23701 gcagctcaaa ttaaagatgt aaccttgtca gaagcaggtg aaaaactgac agaaattgta
    23761 ttaaatgaaa taaaggaaaa ggagaaagaa gaatgaaagt aatcttttta gcagatgtta
    23821 aaggaaaagg taaaaaaggc gaaattaagg aagtaccaac agggtatgcg caaaactttc
    23881 ttatcaaaaa gaatctagcc aaagaagcga ctgctcaagc tgtaggtgaa cttcgtggta
    23941 aacaaaaatc ggaagaaaaa gctcacgctg agatgattgc agaaggaaaa gcaattaaag
    24001 cacaacttga agcagaagaa actgttgtag aatttgttga aaaagttggt ccagatggtc
    24061 gtacctttgg ttctattacc aataagaaga ttgcagaaga attgcaaaag caatttggaa
    24121 ttaagattga taaacgtcat attcaagtac aagctccgat tcgagcggtt ggtttgattg
    24181 atgtgccagt gaaaatctat caagatatca caagtgtaat caatcttcgt gtgaaagaag
    24241 gataagttta caccttcttg acaagattgt aaaaggaagg gaagtctgat ggcagaagta
    24301 gaagagttac gagtacaacc tcaagatatc ttagctgagc aatccgtttt aggggctatc
    24361 tttattgatg agagtaaact tgtttttgtg cgagaataca ttgagtctcg ggactttttt
    24421 aagtatgccc atcgtttgat tttccaagcc atggtcgatt tatccgatcg tggtgatgcc
    24481 atagatgcaa caacggttcg tactatcctt gataatcaag gtgatttaca gaatattggt
    24541 ggcttgtctt acttggttga gattgttaat tctgtgccaa cttctgctaa tgcggagtat
    24601 tatgctaaga ttgttgcaga aaaagcaatg ctacgtcgtt taattgccaa gttgacagag
    24661 tctgtcaacc aagcttacga agcgtcacaa ccagctgatg aaattattgc tcaggcagaa
    24721 aaagggctga ttgatgtcag tgaaaatgca aatcgaagcg ggtttaagaa cattcgagat
    24781 gtgttgaatc tcaactttgg aaatctggaa gctcgctcgc aacaaacgac cgatattaca
    24841 ggtattgcga caggttatcg tgatttggat catatgacaa caggacttca tgaggaggag
    24901 ttgattatct tagcagctcg tccagcagtt ggtaagacag catttgcctt gaatatcgct
    24961 cagaacattg ggactaagtt ggacaaaacg gttgctattt tttcactcga aatgggtgcg
    25021 gaaagcttgg tagatcgtat gttagctgca gaaggcttgg tggagtcaca ttctatccgt
    25081 acagggcaat tgacagatga ggagtggcaa aaatatacta ttgctcaggg taatctagct
    25141 aacgccagta tctatatcga tgatacgcca ggtattcgga ttacagagat tcgttctcgt
    25201 tctcgtaaat tggctcaaga aactggaaat cttggtttga ttttgataga ctatttacaa
    25261 cttatcacgg gaactggtcg agaaaatcgt caacaagaag tttctgaaat ttctcgtcag
    25321 ttgaaaatac tagccaagga attgaaggtt ccagtaatcg ctctgagtca gctttctcgt
    25381 ggtgtagaac aacgtcagga caagagaccg gtcttgtctg atattcgtga atctgggtct
    25441 attgagcagg acgctgatat cgtagctttt ctctatcgcg atgactacta tgaacgtggt
    25501 ggtgaagaag aggagggtat cccaaataat aaggtggaag ttattatcga gaaaaaccgt
    25561 agtggagctc gtggaacagt ggaattgatt ttccaaaaag aatacaataa attttcaagt
    25621 atctcaaaga gggaggcata agatgtcaga tgcatttaca gatgtagcca agatgaaaaa
    25681 aatcaaagaa gaaatcaagg cacatgaggg acaagtcgta gaaatgactt tggagaatgg
    25741 tcgtaagcgc caaaaaaata gattgggtaa gctaattgaa gtttatccat ccctatttat
    25801 tgtggagttt ggggatgtgg aaggagataa acaagttaat gtttacgttg aatcctttac
    25861 ttactcagat attcttacag aaaagaattt gattcattat cttgactaaa gtgagaaatt
    25921 ttctcacttt ttcttttttc tccgaataat ttaggtgaag gcaatcatcg ctttatatta
    25981 tttttcaagg aggaagaatg aaaattttac cgtttatagc aagaggaaca agttattact
    26041 tgaagatgtc agttaaaaag cttgttcctt ttttagtagt aggattgatg ctagcagctg
    26101 gtgatagtgt ctatgcctat tccggaggaa atggatcgat tgcgcgtggg gatgattatc
    26161 ctgcttatta taaaaatggg agccaggaga ttgatcagtg gcgcatgtat tctcgtcagt
    26221 gtacttcttt tgtagccttt cgtttgagta atgtcaatgg ttttgaaatt ccggcagctt
    26281 atggaaatgc gaatgaatgg ggacatcgtg ctcgtcggga aggttatcgt gtagataata
    26341 caccgacgat tggttccatt acttggtcta ctgcaggaac ttatggtcat gttgcctggg
    26401 tgtcaaatgt aatgggagat cagattgaga ttgaggaata taactatggt tatacagaat
    26461 cctataataa acgaattata aaagcaaaca cgatgacagg atttattcat tttaaagatt
    26521 tggatggtgg cagtgttggg aatagtcaat cctcaacttc aacaggcgga actcattatt
    26581 ttaagaccaa gtctgctatt aaaactgaac ccctagttag tgcaactgtg attgattact
    26641 attatcctgg agagaaggtt cattatgatc aaattctcga aaaagacggc tacaagtggt
    26701 tgagttatac ggcttataac ggaagtcgtc gctatataca gctagaggga gtgacttctt
    26761 cacaaaatta tcagaatcaa tcaggaaata tctctagcta tggatccaat aatagttcaa
    26821 ctgtcggttg gaagaaaata aatggtagtt ggtatcattt caaatcaaat ggttctaaat
    26881 caacaggatg gctgaaagac ggttctagct ggtattattt gaaattatct ggtgaaatgc
    26941 agacaggatg gttaaaggaa aatggtttgt ggtattatct gggtagttca ggggcaatga
    27001 aaacaggctg gtaccaggtc tctggtaagt ggtattattc ttactcttca ggcgccttag
    27061 ctgttaatac gacggtggat ggctacagag taaacagtga tggagaacga gtatagaaaa
    27121 ttggagtagg agaaatttcc tgctcttttc tgtaagcagt ttccttgaca tttttcttat
    27181 tttgcgctat catatataca tataatatat gggagtctgt gtacagtctg agaggaagtg
    27241 ttaaacttcg accgcacctg atctgggtaa tgccagcgga gggaacgata cttagtctaa
    27301 ttttgcacct tttccatgta tggtaaaggt ttttcttttt tttaaaagga aaacgagaag
    27361 aggaggttct tatgaaagca agcattgcct tgcaagtttt acccctagca caggggattg
    27421 atcggatagc tgttattgat caggtcattg cttatctgca aactcaagaa gtgacgatgg
    27481 tagtgacacc atttgaaacg gtcttggaag gggagtttga tgagcttatg cgcattctaa
    27541 aagaagcgct ggaagtggca gggcaggagg cagacaatgt ctttgccaat gtcaaaataa
    27601 atgtaggaga gattttaagt attgatgaga aacttgaaaa gtatactgag acgacacatt
    27661 agtctattgg gctttctcgg agtattgtca atctggcagt tagcaggttt tcttaaactt
    27721 ctccccaagt ttatcctgcc gacacctctt gaaattctcc agccctttgt tcgtgacaga
    27781 gaatttctct ggcaccatag ctgggcgacc ttgagagtgg ctttactggg gctgattttg
    27841 ggagttttga ttgcctgtct tatggctgtg ctcatggata gtttgacttg gctcaatgac
    27901 ctgatttacc ctatgatggt ggtcattcag accattccga ccattgccat agctcctatc
    27961 ctggttttgt ggctgggtta tgggattttg cccaagattg tcttgattat cttaacaaca
    28021 acctttccca tcatcgttag tattttggac ggttttaggc attgcgacaa ggatatgctg
    28081 actttgttta gtctgatgcg agcaaaccct tggcaaatcc tgtggcattt taaaatcccg
    28141 gttagcctgc cttactttta tgcaggtctg agggtcagtg tctcctacgc ctttatcaca
    28201 actgtggtat ctgagtggtt gggaggtttt gaaggtcttg gtgtttatat gattcagtct
    28261 aaaaaactgt ttcagtatga taccatgttt gccattatta ttctggtgtc gattatcagt
    28321 cttttgggta tgaagctggt cgatatcagt gaaaaatatg tgattaaatg gaaacgtact
    28381 taaaatagag cgtttttgaa aaagaaaaga ggaaatcaaa atgaagaaaa catggaaagt
    28441 gtttttaacg cttgtaacag ctcttgtagc tgttgtgctt gtggcctgtg gtcaaggaac
    28501 tgcttctaaa gacaacaaag aggcagaact taagaaggtt gactttatcc tagactggac
    28561 accaaatacc aaccacacag ggctttatgt tgccaaggaa aaaggttatt tcaaagaagc
    28621 tggagtggat gttgatttga aattgccacc agaagaaagt tcttctgact tggttatcaa
    28681 cggaaaggca ccatttgcag tgtatttcca agactacatg gctaagaaat tggaaaaagg
    28741 agcaggaatc actgccgttg cagctattgt tgaacacaat acatcaggaa tcatctctcg
    28801 taaatctgat aatgtaagca gtccaaaaga cttggttggt aagaaatatg ggacatggaa
    28861 tgacccaact gaacttgcta tgttgaaaac cttggtagaa tctcaaggtg gagactttga
    28921 gaaggttgaa aaagtaccaa ataacgactc aaactcaatc acaccgattg ccaatggcgt
    28981 ctttgatact gcttggattt actacggttg ggatggtatc cttgctaaat ctcaaggtgt
    29041 agatgctaac ttcatgtact tgaaagacta tgtcaaggag tttgactact attcaccagt
    29101 tatcatcgca aacaacgact atctgaaaga taacaaagaa gaagctcgca aagtcatcca
    29161 agccatcaaa aaaggctacc aatatgccat ggaacatcca gaagaagctg cagatattct
    29221 catcaagaat gcacctgaac tcaaggaaaa acgtgacttt gtcatcgaat ctcaaaaata
    29281 cttgtcaaaa gaatacgcaa gcgacaagga aaaatggggt caatttgacg cagctcgctg
    29341 gaatgctttc tacaaatggg ataaagaaaa tggtatcctt aaagaagact tgacagacaa
    29401 aggcttcacc aacgaatttg tgaaataatg acagaaatta gactagagca cgtcagttat
    29461 gcctatggtc aggagaggat tttagaggat atcaacctac aggtgacttc aggcgaagtg
    29521 gtttccatcc taggcccaag tggtgttgga aagaccaccc tctttaatct aatcgctggg
    29581 attttagaag ttcagtcagg gagaattgtc cttgatggtg aagaaaatcc caaggggcgc
    29641 gtgagttata tgttgcaaaa ggatctgctc ttggagcaca agacggtgct tggaaatatc
    29701 attctgcccc tcttgattca aaaggtggat aaggcagaag ctatttcccg agcggataaa
    29761 attcttgcga ccttccagct gacagctgta agagacaagt atcctcatga acttagcggt
    29821 gggatgcgcc agcgtgtagc cttactccgg acctaccttt ttgggcacaa gctctttctc
    29881 ttagatgagg cctttagcgc cttggatgag atgacaaaga tggaactcca cgcttggtat
    29941 cttgagattc acaagcagtt gcagctaaca accctgatca tcacgcatag tattgaggag
    30001 gccctcaatc tcagcgaccg catctatatc ttgaaaaatc gccctgggca gattgtttca
    30061 gaaattaaac tagattggtc tgaagatgag gacaaggaag tccaaaagat tgcctacaaa
    30121 cgtcaaatct tggcagaatt aggcttagat aagtagaaaa atagggagtt ggtgaagatt
    30181 atcctttacc agcgcccttt ttcttttaaa aatgagaaaa tttcggtata atagtcaaac
    30241 aaggtcaagg tttaaagaga gaggtgggtt tgttatgaga tttaaaaata catcggatca
    30301 tattgaggcc tacatcaagg cgattttaga tcaatctggt atcgtggagt tgcaacggag
    30361 tcagttggca gatacctttc aggttgttcc tagtcagatt aactacgtga tcaagacacg
    30421 ctttacggaa agtagaggct acttggttga aagtaagcgt ggtggcggag gctacattcg
    30481 tataggacgg attgagtttt ctagtcatca tgaaatgctc cgggagctgc tttactcgat
    30541 tggtgagcga gtcagtcaag aaatttatga ggatattctc cagcttttgg ttgagcagga
    30601 attgatgacc aagcaggaga tgaatttgct agaatcagta gctttggatc gcgttttagg
    30661 agaagaagct ccagttgttc gagcaaacat gctacgtcag atcatacaag aggtagatag
    30721 aaaagggaag taagatgaac tattcaaaag cattgaatga atgtatcgaa agtgcctaca
    30781 tggttgctgg acattttgga gctcgttatc tagagtcttg gcacttgttg attgccatgt
    30841 ctaatcacag ttatagtgta gcaggggcaa ctttaaatga ttatccgtat gagatggacc
    30901 gtttagaaga ggtggctttg gaactgactg aaacggacta tagccaggat gaaaccttta
    30961 cggaattgcc gttctcccgt cgtttgcagg ttctttttga cgaagcagag tatgtagcgt
    31021 cagtggtcca tgctaaggtg ctagggacag agcacgtcct ctatgcgatt ttgcatgatg
    31081 gcaatgcctt ggcgactcgt atcttggaga gggctggatt ttcttatgaa gacaagaaag
    31141 atcaggtcaa gattgctgct cttcgtcgaa atttagaaga acgggcaggc tggactcgtg
    31201 aagatctcaa ggctttacgc caacgccatc gtacagtagc tgacaagcaa aattctatgg
    31261 ccaatatgat gggcatgccg cagactccta gtggtggtct cgaggactat acgcatgatt
    31321 tgacagagca agcgcgttct ggcaagttag aaccagtcat cggtcgggac aaggaaatct
    31381 cacgtatgat tcaaatcttg agccggaaga ctaagaacaa ccctgtcttg gttggggatg
    31441 ctggtgtcgg gaaaacagct ctggcgcttg gtcttgccca gcgtattgct agtggtgacg
    31501 tgcctgcgga aatggctaag atgcgcgtgt tagaacttga tttgatgaat gtcgttgcag
    31561 ggacacgctt ccgtggtgac tttgaagaac gcatgaataa tatcatcaag gatattgaag
    31621 aagatggcca agtcatcctc tttatcgatg aactccacac catcatgggt tctggtagcg
    31681 gaattgattc gactctggat gcggccaata tcttgaaacc agccttggcg cgtggaactt
    31741 tgagaacggt tggtgccacc actcaggaag aatatcaaaa acatatcgaa aaagatgcgg
    31801 cactttctcg tcgtttcgct aaagtgacga ttgaagaacc aagtgtggca gatagtatga
    31861 ctattttaca aggtttgaag gcgacttatg agaaacatca ccgtgtacaa atcacagatg
    31921 aagcggttga aacagcggtt aagatggctc atcgttattt aaccagtcgt cacttgccag
    31981 actctgctat cgatctcttg gatgaggcgg cagcaacagt gcaaaataaa tcaaagcatg
    32041 taaaagcaga cgattctgac ttgagtccag ctgacaaggc cttgatggat ggcaagtgga
    32101 aacaggcagc ccagctaatc gcaaaagaag aggaagtgcc tgtctataaa gacttggtga
    32161 cagagtctga tattttgacc accttgagtc gcttgtcagg tatccccgtt caaaaactga
    32221 ctcagactga tgccaagaaa tacttaaact tggaagctga actgcacaaa cgtgtcatcg
    32281 gtcaagacca agctgtttca agtattagtc gtgccattcg ccgcaatcag tcaggaattc
    32341 gcagtcacaa gcgtccgatc ggttccttta tgttcctagg acccacaggt gtcggtaaga
    32401 ccgagttggc caaggctttg gcagaagttc tctttgatga cgaatcagcc cttatccgct
    32461 ttgatatgag tgagtatatg gagaaattcg cagccagccg tctaaatgga gctcctccag
    32521 gctatgtggg ttacgaagaa ggtggggagt tgacagagaa ggttcgcaat aaaccctatt
    32581 ccgttctcct ctttgatgag gtagagaagg cccatccaga tatctttaat gttctcttgc
    32641 aggttctgga tgacggtgtc ttgacagata gcaagggacg caaggtcgat ttttcaaata
    32701 ccattatcat tatgacatcg aacttaggtg cgactgccct tcgtgatgat aagactgttg
    32761 gttttggggc taaggatatt cgttttgacc aggaaaatat ggaaaaacgc atgtttgaag
    32821 aactgaaaaa agcttataga ccggaattca tcaaccgtat tgatgagaag gtggtcttcc
    32881 atagcctatc tagtgaccat atgcaggaag tggtgaagat tatggtcaag cctttagtgg
    32941 caagtttggc tgaaaaaggc attgacttga aattacaagc ttcagctctg aaattgttag
    33001 caaatcaagg atatgaccca gagatgggag ctcgcccact tcgcagaacc ctgcaaacag
    33061 aagtggagga caagttggca gaacttcttc tcaagggaga tttagtggca ggcagcacac
    33121 ttaagattgg tgtcaaagca ggccagttaa aatttgatat tgcataaaag aataaaagta
    33181 ccagcatctg accataagtc acagtggagt gaaactcaat gaaaatcaaa gagcaaacta
    33241 ggcagctagc cgcaggttgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg
    33301 tttgaagaga tttttgaaga gtataagctg caagatgaat gattttcttg tattgacgtt
    33361 gttgttgaca aaaagtagcg gataaatgaa atccattcca ttatcataga tgataggctg
    33421 gtaggaaatt ttcaaatagc atacagaaaa tagatgtatg gagttctggt agtagaaagg
    33481 gagagagatg aacattttag ttgcagatga cgaggaaatg attagagaag gaattgcagc
    33541 atttctgaca gaagagggtt atcatgtcat tatggctaag gatggacaag aggtcttgga
    33601 aaaatttcaa gatctcccta tccatctcat ggtactggat ttaatgatgc ctaggaagag
    33661 tggttttgaa gtgttaaaag aaatcaatca aaagcacgat attcctgtca tcgtcttgag
    33721 tgctctggga gatgaaacta ctcagtcaca ggtatttgat ctctatgctg atgatcatgt
    33781 gacaaaacct ttttctttgg tactgcttgt caagcgtatt aaggcgctta tcagacgtta
    33841 ctacgtcata gaggatcttt ggcgatatca ggatgtaaca gtggatttta cctcttacaa
    33901 agcacattat aaaaatgaag aaattgatct caaaccaaag gaattactgg tactaaagtg
    33961 tttgattcag cataaaaatc aagttttaag tagagagcag atattggaag aaatttcaaa
    34021 agatgtagct gatttacctt gtgatagggt cgttgatgtc tatattcgta ctcttcgcaa
    34081 aaaattagct ttagattgta tcgtgactgt gaaaaatgtt gggtataaga ttagcttatg
    34141 ataaaaaatc ctaaattatt aaccaagtct tttttaagaa gttttgcaat tctaggtggt
    34201 gttggtctag tcattcatat agctatttat ttgacctttc ctttttatta tattcaactg
    34261 gagggggaaa agtttaatga gagcgcaaga gtgtttacgg agtatttaaa gactaagaca
    34321 tctgatgaaa ttccaagctt actccagtct tattcaaagt ccttgaccat atctgctcac
    34381 cttaaaagag atattgtaga taagcggctc cctcttgtgc atgacttgga tattaaagat
    34441 ggaaagctat caaattatat cgtgatgtta gatatgtctg ttagtacagc agatggtaaa
    34501 caggtaaccg tgcaatttgt tcacggggtg gatgtctaca aagaagcaaa gaatattttg
    34561 cttttgtatc tcccatatac atttttggtt acaattgctt tttcctttgt tttttcttat
    34621 ttttatacta aacgcttgct caatcctctt ttttacattt cagaagtgac tagtaaaatg
    34681 caagatttgg atgacaatat tcgttttgat gaaagtagga aagatgaagt tggtgaagtt
    34741 ggtgaagttg gaaaacagat taatggtatg tatgagcact tgttgaaggt tattcatgag
    34801 ttggaaagtc gtaatgagca aattgtaaaa ttgcaaaatc aaaaggtttc ctttgtccgt
    34861 ggagcatcac atgagttgaa aaccccttta gccagtctta gaattatcct agagaatatg
    34921 cagcataata ttggagatta caaagatcat ccaaaatata ttgcaaagag tataaataag
    34981 attgaccaga tgagccgctt attggaagaa gtactggagt cttctaaatt ccaagagtgg
    35041 acagagtgtc gtgagacctt gactgttaag ccagttttag tagatatttt atcacgttat
    35101 caagaattag ctcattcaat aggtgttaca attgaaaatc aattgacaga tgctaccagg
    35161 gtcgtcatga gtcttagggc attggataag gttttgacaa acctgattag taatgcaatt
    35221 aaatattcag ataaaaatgg gcgcgtaatc atatccgagc aagatggcta tctctctatc
    35281 aaaaatacat gtgcgcctct aagtgatcaa gaactagaac atttatttga tatattctat
    35341 cattctcaaa tcgtgacaga taaggatgaa agttctggtt tgggtcttta cattgtgaat
    35401 aatattttag aaagctatca aatggactat agttttctcc cttatgaaca cggtatggaa
    35461 tttaagatta gcttgtagac agattagttt tttattaaag ttcatatagg gttaacataa
    35521 gtgtgttatt ctttgtgtag ataaaagaaa ggatactaat atggtattag cgattatttt
    35581 agtaacattc tttattcgat tgattttttt aaagcgttcg atagagaatg agaaacgaat
    35641 ccttagcaat ggcgggaaag aatttggagt tgagaataca aaacgattaa ctatggctca
    35701 tattgttttt tatctctctt gcttggttga ggcaatggtg cacaagacaa tttttgatgg
    35761 catgggcatg gttggtttgg tcttgcttat tttttctatg ctgatgttga tgttggtgat
    35821 tcacttgttg ggagatattt ggacagtgaa gcttatgctt gtcaataatc acaaatatgt
    35881 agatcatatc ttgtttagga cagtaaaaca ccctaattac tttttaaata ttttacctga
    35941 gttgattggc ttgaccttgt tgagtcatgc ttatgtgact tttgttttag tttttccagt
    36001 ttatgcagtt attttgtatc gacgaatagc tgaagaggaa aagctattac atgaagttat
    36061 aatcccaaat ggaagcataa agagataaat acaaaattcg atttatatac agttcatatt
    36121 gaagtgatat agtaaggtta aagaaaaaat atagaaggaa ataaacatgt ttgcatcaaa
    36181 aagcgaaaga aaagtacatt attcaattcg taaatttagt attggagtag ctagtgtagt
    36241 agttgctagc ttgttcttag gaggagtagt ccatgcagaa ggggttagaa gtgagaataa
    36301 ccccacggtt acatctagtg ggcaagatat atcgaagaag tatgctgatg aagtcaagtc
    36361 acatctagaa aaaatattga gtgagatcca aacaaattta gatagaagta aacatatcaa
    36421 aactgtaaat ctaattaaca aattgcaaga cattaagaga acgtatttgt atgaattaaa
    36481 tgttttagaa gataagtcga aagctgagtt gccgtcaaaa ataaaagcag agttagacgc
    36541 agcttttgag cagtttaaaa aagatacatt accaacagaa ccaggaaaaa aggtagcaga
    36601 agctaagaag aaggttgaag aagctgagaa aaaagccaag gctcaaaaag aagaagatta
    36661 ccgtaactac ccaaccatta cttacaaaac gcttgaactt gaaattgctg agtccgatgt
    36721 gaaagttaaa gaagcggagc ttgaactagt aaaaaaggaa gctgacgaat ctcgaaacga
    36781 gggcacaatt aaccaagcaa aagcgaaagt tgagagtgaa caagctgagg ctacaaggtt
    36841 aaaaaaaatc aagacagatc gtgaaaaagc agaagaagaa gctaaacgaa gagcagatgc
    36901 taaagagcaa gatgaatcaa agaggcgaaa gagtcgggta aaacgaggag atcttggaga
    36961 gccagcaaca cctgataaaa aagaaaatga tgcgaagtct tcagattcta gcgtaggtga
    37021 agaaactctt ccaagcccat ccctgaaacc aggaaaaaag gtagcagaag ctgagaagaa
    37081 ggttgaagaa gctgagaaaa aagccaagga tcaaaaagaa gaagatcacc gtaactaccc
    37141 aaccattact tacaaaacgc ttgaacttga aattgctgag tccgatgtgg aagttaaaaa
    37201 agcggagctt gaactagtaa aagaggaagc taagggatct cgaaacgagg aaaaagttaa
    37261 gcaagcaaaa gcggaagttg agagtaaaaa agctgaggct acaaggttag aaaaaatcaa
    37321 gacagatcgt aaaaaagcag aagaagctaa acgaaaagca gcagaagaag ataaagttaa
    37381 agaaaaacca gctgaacaac cacaaccagc gccggctcct caaccagaaa aaccagctcc
    37441 agctccaaaa ccagagaatc cagctgaaca accaaaagca gaaaaaccag ctgatcaaca
    37501 agctgaagaa gactatgctc gtagatcaga agaagaatat aatcgcttga ctcaacagca
    37561 accgccaaaa actgaaaaac cagcacaacc atctactcca aaaacaggct ggaaacaaga
    37621 aaacggtatg tggtacttct acaatactga tggttcaatg gcgacaggat ggctccaata
    37681 caatggttca tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggttccaata
    37741 caatggttca tggtactacc tcaacgctaa tggtgatatg gcgacaggat ggctccaata
    37801 caatggttca tggtactacc taaacagcaa tggtgctatg gtaacaggat ggctccaata
    37861 caatggttca tggtactacc tcaacgctaa cggttcaatg gcaacagatt gggtgaaaga
    37921 tggagatacc tggtactatc ttgaagcatc aggtgctatg aaagcaagcc aatggttcaa
    37981 agtatcagat aaatggtact atgtcaatgg ctcaggtgcc cttgcagtca acacaactgt
    38041 agatagctat agagtcaatg ccaatggtga atgggtaaac taaacctaat ataactcgtt
    38101 aatgctggct tcctgtaaga actctttaaa gtattcccta caaataccat atcctttcag
    38161 tagataatat acccttgtag gaagtttaga ttaaaaaata actctgtaat ctctagccgg
    38221 atttatagcg ctagagacta cggagttttt ttgatgagga aagaatggcg gcactcaaga
    38281 gactctttaa gagagttacg ggttttaaac tattaagcct tctccaattg caagagagct
    38341 tcaatctctg ccagggtgct agcttgcgaa atggctccac ggagtttggc agcgccagat
    38401 gttccacgga ggtagtgagg agcgaggcca cggaattcac gaactgcgac gttttctcct
    38461 ttgaggttaa tcaatcgttt caagtgttcg taggcgatct tcatcttgtc ttcaaaggtc
    38521 aaatcaggta ggatttctcc tgtttcaaag taatggttga tttggttgaa gaggtaagga
    38581 tttcccatgg cagctcggcc aatcatgact gcgtcagcac caacttcttc gatgcgttgc
    38641 ttggcttctt ggacagtacg gatatcaccg ttggcgatga atggaatctt ggttagagct
    38701 tgggcaacct tgtacagggt ctcaaggtct gcgtggccag tatacatttg ttcacgggta
    38761 cggccatgca tggcgagggc agaaacacct gcagcctcag cagcgagggc attttctact
    38821 gccagagatg ggtccgccca gccggtacgc attttgacag taagtgggat atcaaggaca
    38881 gactggacct tgttgatgat agagtagatc ttgtcaggat ccttgagcca catagctcca
    38941 gcttcgttct tcacgatttt gttgacaggg cagcccatgt tgatatcgac gatatcggtc
    39001 ttggtgtttt cttggatgaa ttctgctgcg cgtgctaggc tgtcttcatc gctaccaaaa
    39061 agttggatag agacagggtt ttcgccctca tcgatatgaa gcatgtgcag ggttttttcg
    39121 ttgttgtatt ggattccctt gtcagagacc atttccatta caacgagtcc agctccgagc
    39181 tcttttgcga tggtacgaaa ggctgagttg gtcacgccag ccataggcgc taaaacggta
    39241 cgattgggaa tctcaacatt gccaatcata aaaggtgtat taagatttgt cacgaatgag
    39301 ttcctccagg tccttttcat caaagttgta agtagtttgg cagaattgac aagtgatttc
    39361 taccccgtgg tcttcctctt tcatttcctg taagtctgag cttggaaggc tggcaagagc
    39421 gttcataaag cgttcatggc tacagtcaca ttggaaacgg atttcttctt cagaaagacg
    39481 cttgtaggct tcgtccccgt agatagcctt gaggagggct tcgatatggt cgtcgctttc
    39541 gagaagagta gagatagctg gcatttcttg gatgcgtttt tcaaagcgag caatctcttc
    39601 tttcttggct cctggcaaga cttgaactag gaaaccacct gcaaccttga ccttgtcttc
    39661 ctcgtccaaa aggacattga ggccgaccgc tgaaggcgtt tgttggcttt cagtaaggta
    39721 aaaggcaagg tcttcaccga tttctccaga gatgagggga gttatagagt tgtaaggatt
    39781 tccagcaccg tagtctgtga taacgaggaa ttgaccattt ccaacaaaag gtccgactag
    39841 gacttcacca gtcgcagtct ttttgatgtc aacaccagga ttttgaacat agcctttgac
    39901 gttccccttg gtatcagcga cggtgataat agcacctaga gagctagagc ccaacacctt
    39961 aactgtaagt ttggtatttc ctttttcatt ggctgcgaga atctggctgg cgataagagt
    40021 tcgaccaagc gctacagttg agctagcttg ggtttgatgt ttttcttgag cagtgcggac
    40081 ggtttccgtg ctatcaagga caaaagcacg aaaggctccg ctttctgata tagttttaat
    40141 aattttatcc atagctacta ttttagcata aaaatgccca aagggggagc cgtgtgttta
    40201 ctgattttca ggataatgga ccaggaaatc agcatgaaaa taaaaagaga aacagattat
    40261 tttagcattt gtaagattta tgctatgctt aaggtagaaa atgaaagggg taacaaatgt
    40321 atttaggaga tttgatggag aaagccgagt gtggtcaatt ttcaatcctt tcctttctat
    40381 tacaagagtc tcagacgacc gtcaaggctg taatggaaga aacaggattt tcaaaagcaa
    40441 ccctaaccaa atatgtcacc ctgctcaatg acaaggcttt ggatagtggc ttagagctgg
    40501 ctattcactc agaagatgaa aatctgcgtc tgtctatcgg tgcagctacc aaggggagag
    40561 atattcggag cttgtttttg gagagtgctg ttaaatacca gattttggtt tatcttctct
    40621 accaccaaca gtttttagcc catcagctgg ctcaagaatt ggtgattagc gaggctacgc
    40681 ttggtcgtca cttggctggt ttaaatcaga ttttgtcaga atttgattta tccatccaaa
    40741 atggacgttg gcgaggtcca gagcatcaga ttcgctattt ctatttctgt cttttccgaa
    40801 aggtctggtc gagtcaggaa tgggaaggtc acatgcagaa accagagaga aaacaggaga
    40861 ttgccaattt agaggaaatc tgcggtgcaa gtttgtctgt ggggcagaaa ttggacttgg
    40921 ttctctgggc tcacatcagt caacaacgtc ttcgggtcaa tgcttgtcag tttcaagtca
    40981 tagaagagaa aatgcgaggg tattttgaca atattttcta ccttcgtttg ctgagaaagg
    41041 ttccgtcctt ttttgctggg caacatattc cactaggagt tgaggatggt gagatgatga
    41101 tattcttctc ttttctccta tctcatcgca ttcttcctct tcatactatg gagtatattc
    41161 ttggttttgg agggcagttg gcagatttac tgacgcaatt gattcaagaa atgaagaagg
    41221 aggaactatt gggggattat acagaggacc atgtcaccta tgaactcagt cagctttgtg
    41281 ctcaagtcta tctctataag ggctatattt tacaggatcg ctacaagtac cagttagaga
    41341 atcgtcatcc atatttactg atggaacatg attttaaaga gacagcagag gagatttttc
    41401 atgctctacc tgcttttcaa caggggacag atttagataa gaagattctc tgggaatggc
    41461 tccagttaat cgaatatatg gctgaaaacg gtggccagca tatgcggatt ggtctggatt
    41521 tgacatctgg ttttcttgtc ttttcaagga tggcagccat tttgaaacgg tatttggaat
    41581 acaatcgttt tattaccatt gaagcctatg accctagtcg gcattatgat ttgctggtta
    41641 ccaataaccc gattcataag aaggaacaga caccagtcta ttatttaaaa aatgacttgg
    41701 atatggagga tttggtagcg attcgccagt tattattcac ttaaaaggct tggttaatcc
    41761 aggtcttttt tgtgaaattc acacaatctc ctcacatttt tttaaaaatt aaaaaaagtt
    41821 gataaacaag aaagcgcttt attttgtata ctagtaagtg taaagaggaa acacctcaag
    41881 atctttatca ggaggacagt acatgtcaca agaaaaatac atcatggcca ttgaccaggg
    41941 aactacaagt tctcgtgcca tcattttcaa caaaaaaggg gaaaaggtta gctcgagtca
    42001 aaaagagttt acccagattt tccctcaggc aggttgggtt gagcacaatg ccaatgaaat
    42061 ttggaactct gttcagtcag ttattgcggg tgctttcatc gaaagtggtg tcaagccaaa
    42121 tcaaatcgag gcaatcggga ttaccaacca acgtgaaaca acggttgtct gggataagaa
    42181 aacaggactt cctatctaca atgctatcgt ttggcagtca cgccagacag cacctttggc
    42241 tgagcaacta aaaagccaag gttatgtgga aaaattccat gaaaagactg gtttgattat
    42301 tgatgcttac ttctctgcta ccaaggttcg ttggattttg gatcatgtag aaggtgctca
    42361 agagcgagca gaaaaagggg aattgctctt tggtactatc gatacttggt tggtttggaa
    42421 attgactgac ggtgcggctc acgtgactga ctactcaaat gcagctcgta ccatgcttta
    42481 taacattaaa gaactcaaat gggatgatga gattttggaa atccttaaca ttccgaaggc
    42541 tatacttcca gaagttcgtt ctaactccga aatctacggc aagacagctc cattccattt
    42601 ctacggtgga gaggtgccaa tctcaggtat ggctggggac caacaagcag ccctctttgg
    42661 acagttggct tttgagccag gtatggttaa gaatacttat ggaacaggct ctttcatcat
    42721 catgaatact ggggaagaga tgcagttgtc tgaaaacaac ctcttgacaa ccattggtta
    42781 cggaatcaac ggtaaggttt attatgcctt ggaaggttct atcttcatcg caggaagtgc
    42841 tattcagtgg cttcgtgacg gtcttcgcat ggttgaaaat tcaccagaat ctgaaaaata
    42901 cgctcgtgat tctcacaaca acgatgaagt ttatgtcgtt ccagccttta caggtctagg
    42961 cgctccatac tggaaccaaa atgctcgtgg ttccgtcttt ggtttgactc gtggaacaag
    43021 caaagaagac tttatcaagg cgactttgca atctattgct tatcaagtgc gtgatatcat
    43081 cgacaccatg caagtggata ctcagaccgc cattcaagta ctgaaggtgg atggtggtgc
    43141 agccatgaac aacttcctca tgcagttcca ggcggatatt ttaggcattg acattgcacg
    43201 tgctaaaaac ttggaaacaa cagctctagg agcggccttc ctagcaggtt tgtcagtagg
    43261 gtactggaaa gacttggacg agttgaaact cttgaacgag acaggagaac tctttgagcc
    43321 atctatgaac gaatctcgca aggaacaact ctacaagggc tggaagaagg ctgtgaaagc
    43381 aactcaagtc tttgcggaag tagacgacta atactggcag aataaagcga tttatttaga
    43441 aagtgtgtaa atatggaatt ttcaaaaaaa acacgtgaat tgtcaattaa aaaaatgcag
    43501 gaacgcaccc tggacctctt gattatcggt ggaggaatca caggagctgg tgtagccttg
    43561 caggcggcag ctagcggtct tgagactggt ttgattgaaa tgcaagactt tgcagaagga
    43621 acatctagtc gttcaacaaa attggttcac ggaggtcttc gctacctcaa acaattcgac
    43681 gtagaagtgg tctcagatac ggtttctgaa cgtgcagtgg ttcaacaaat cgctccacac
    43741 attccaaaac cagatccaat gctcttacca gtttacgatg aagatggagc aacctttagc
    43801 ctcttccgtc ttaaagtagc catggacttg tacgacctct tggcaggtgt tagcaacaca
    43861 ccaactgcga acaaggtttt gagcaagaaa gaagtcttgg aacgccagcc aaacttgaag
    43921 aaggaaggct tggtaggagg tggagtgtat cttgacttcc gtaacaacga tgcgcgtctc
    43981 gtgattgaaa acatcaaacg tgccaaccaa gacggtgccc tcattgccaa ccacgtgaag
    44041 gcagaaggct tcctctttga cgaaagtggc aagattacag gtgttgtagc tcgtgatctc
    44101 ttgacagatc aagtatttga aatcaaggcc cgtctggtta ttaatacaac aggtccttgg
    44161 agtgataaag tacgtaattt gtctaataag ggaacgcaat tctcacaaat gcgcccaact
    44221 aagggagttc acttggtagt ggattcaagc aaaatcaagg tttcacagcc agtttacttc
    44281 gacacaggtt tgggtgacgg tcgtatggtc tttgttctcc cacgtgaaaa caagacttac
    44341 tttggtacaa ctgatacaga ctacacaggt gatttggagc atccaaaagt aactcaagaa
    44401 gatgtagatt atctacttgg cattgtcaac aaccgctttc cagaatccaa catcaccatt
    44461 gatgatatcg aaagcagctg ggcaggtctt cgtccattga ttgcagggaa cagtgcctct
    44521 gactataatg gtggaaataa cggtaccatc agtgatgaaa gctttgacaa cttgattgcg
    44581 actgttgaat cttatctctc caaagaaaaa acacgtgaag atgttgagtc tgctgtcagc
    44641 aagcttgaaa gtagcacatc tgagaaatat ttggatccat ctgcagtttc tcgtgggtct
    44701 agcttggacc gtgatgacaa tggtcttttg actcttgctg gcggtaaaat cacagactac
    44761 cgtaagatgg ctgaaggagc tatggagcgc gtggttgaca tcctcaaagc agaatttgac
    44821 cgtagcttta aattgatcaa ttctaaaact taccctgttt caggtggaga attgaaccca
    44881 gcaaatgtgg attcagaaat cgaagccttt gcgcaacttg gagtatcacg tggtttggat
    44941 agcaaggaag ctcactatct ggcaaatctt tacggttcaa atgcaccgaa ggtctttgca
    45001 cttgctcaca gcttggaaca agcgccagga ctcagcttgg cagatacctt gtcccttcac
    45061 tatgcaatgc gcaacgagtt ggctcttagc ccagttgact tccttcttcg tcgtaccaac
    45121 catatgctct ttatgcgtga tagcttggat agcatcgttg agccagtttt ggatgaaatg
    45181 ggacgattct atgactggac agaagaagaa aaagcaactt accgtgctga tgtcgaagca
    45241 gctctcgcta acaacgattt agcagaatta aaaaattaag aaaaaataaa agaggtggag
    45301 ggcagcattc cttgtcgccc gtcccttctt tttaatggag acagaaagat gatgaatgaa
    45361 ttatttggag aatttctagg gactttaatc ctgattcttc taggaaatgg tgttgttgca
    45421 ggtgtggttc ttcctaaaac caagagcaat agctcaggtt ggattgtgat tactatgggt
    45481 tgggggattg cagttgcggt tgcagtcttt gtatctggca agctcagtcc agctcattta
    45541 aatccagctg tgacagttgg tgtagcttta aaaggtgact tgccttgggc ttccgttttg
    45601 ccttatatct tagcccagtt cgcaggggcc atgctgggtc agattttggt ttggttgcaa
    45661 ttcaaacctc actatgaggc agaagaaaat gcaggcaata tcctggcaac cttcagtact
    45721 ggaccagcca tcaaggatac tgtatcaaac ttgattagcg aaatccttgg aacctttgtt
    45781 ttggtgttga caatctttgc tttgggtctt tacgattttc aggcaggtat cggaaccttt
    45841 gcagtgggaa ctttgattgt cggtatcggt ctatcactag gtgggacaac aggttatgcc
    45901 ttgaacccag ctcgtgacct tggacctcgt atcatgcaca gcatcttgcc aattccaaac
    45961 aagggagacg gagactggtc ttacgcttgg attcctgttg taggccctgt tatcggagca
    46021 gccttggcag tgcttgtatt ctcacttttc tagtttatac tcttcgaaaa tcaaattcaa
    46081 accacgtcag cgtcgcctta ccgtactcaa gtacagcttg cggctagctt cctagtttgc
    46141 tctttgattt tcattgagta ttagaaaaca attatgttga tagagcttgg gcaagagccc
    46201 aatttcagca aaaaatgaag taaatcttct cataataaaa cgcatcatat caagcacgaa
    46261 aattccacga ggtcaactac agtcagaaag ctgaacaaca agccaaaacg cccaaaaaag
    46321 gcggcaaaaa gcaagcacct gcaagcaacg tgccgaaatg gtcaaatcct gattatgtca
    46381 acgaattaga cccaaaaatc gttgatatgc tagtagaatt tcacaagtca caaggcactt
    46441 tggaaactcc cgaggcgcaa gcagaaatcg cccaaaaacg tgaagaaatc gagcaaagga
    46501 gagctgagct tgaggataaa aaacaagagc ttttgaaccg cttgaacaaa tagagtttcg
    46561 caagtattat gcttacaaat tacttgagca attaactaaa atataaaccc tgcctagata
    46621 taaaggcagg gtttatattt tagaaattca cgtaggttgt tacggttttt acatacccag
    46681 tatagtttga gtttctatag tattcagtga taaacttcca ttttctttga gcaacatgga
    46741 tataagtact tgttatgtag tatggatatg ggctttgtga atccaagtaa gactgataag
    46801 cttgtatacc aaaatatgct ccaccaatta ttgcacccca tggacccccc aataaagcac
    46861 ctatcctacc aatcatataa ctgattccag caccagtcat gaagttagcg aatgtgttag
    46921 cttgtttatt cccatgtatt gtgttgacgt aattccaaac attaggatcg tatgatctaa
    46981 aagatatatt taggtcgatt tcattctttt gataagccat ataaaatgcc ccattgatat
    47041 agacgccgtc agcacgtcgt tcaatagtgt ctacacttcc atctggattg acaacctcaa
    47101 gaacttcatc gcttaaaata tttacttgcg tatctccgaa ccgcactgat gagccattct
    47161 caaactgagc ctcaccagat acaactttag agtttgccga taagctatca tcagcaaaaa
    47221 caacaagcga cggggaaatg ctagacatac agaaaacaga cataactagc aaacacatgc
    47281 atttaaacat cttagacata acggaaactc ttttgtattt ttgatttttt tcaactttta
    47341 ttatacaata aaaccaaata aaaagaaagc ggtaacaata tgcttaatgc gaaaattttt
    47401 tatatatttt tatgtttgat cgttatcgaa actacaggct tgttgttgtt gaaaagaggt
    47461 ctcgaaatgg gttatttaga cacagaagct attatcctcg cagttttttc atttgctttt
    47521 tacaacctat gttcattcgc ttgggtctgc tctacaataa aaaataaata gacgtatttt
    47581 caaaaaaaaa aaaatgaata tttatattag caaaatgacg atttaaatcg tcgttttttt
    47641 gtagtacgac gggcatgtcg tatatctgag gtgtaagtcc tcggtgggca cctgctaccg
    47701 gtgaacccaa tagcgattcc caagcctgac tatcgtgagg tagcggatta aaacggtctg
    47761 gggatagacc gttttaagtc tgacgcagga aataagaatc gtcagaggaa gggatagcga
    47821 aatcgtggct ctacgaacag gaacgtgata gtaaggcgta tatagcggat aaggaggctt
    47881 caaactctaa agtccaaaaa ggtagtcgta acctatatgt gtaaatcacg agagtaattg
    47941 aattcggact aaggtttgtg tgaaaaagat aaatctttct agagtctaaa gactctgcgt
    48001 cagatttcct attttcactg taacctttta acgtcctcat atcttgtata aacgaggaaa
    48061 gatgtacgac ttatcccgtg aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat
    48121 gagaagtcag ccgagcccat agtagtgagg aaacttctgt agtggaagtg gagcgaaggg
    48181 gtgaatactc aaacagtctg gggagagact gtttgaggtc tgtcgctaga aagagaaaac
    48241 gacagatcga agtaatccta cttcacttgt gtctgtaaaa tgagtggtct gatagaactg
    48301 gactttgaca cgtaatgact agatgaaggt tcaccaatat aagatgtccc tcaggcacca
    48361 aaacaagaaa ggaatacgca catgtcaaaa ctgctagata agatattatc acgcgaaaat
    48421 atgctggaag cctacaatca agtaaaatcc aataaaggct cagctgggat tgatggaatg
    48481 actatcgaag agatggataa ttatctcaga caaaactggc gcttgactaa ggaactgata
    48541 aaacagagaa aatataagcc tcaaccagtt cttaaagttg agatacctaa accagacgga
    48601 ggcatccgtc aactaggaat ttcaacagtt atggatagaa tgattcaaca ggccattgtc
    48661 caagtcatga gccccatttg tgaaccccat ttctcagata cgagttatgg tttcagacca
    48721 aataggtcat gtgaaaaagc catcatgaag ctcttagaat acttaaatga cggctatgag
    48781 tggatagtgg atatagacct agagaaattt ttcgatacag ttcctcaaga tagattgatg
    48841 tccttagtac ataacattat cgaagacgga gatacggaat ccttgattcg taagtatctt
    48901 cattcaggtg ttatcattaa tggtcaacgt tataaaacac tagttggtac accacaggga
    48961 ggaaatttat ctcctctctt atccaatatc atgcttaatg aattggacaa ggaattagaa
    49021 aagaggggac ttcgatttgt gcgctacgca gatgattgtg tgattacggt cgtaagcgag
    49081 gcagccgcta agcgtatgat gtattcagtc agtcgtttta ttgaaaaacg gctaggtttg
    49141 aaagcaaaca tgaccaagac taagattacc agaccaagag agttgaaata tctaggtttt
    49201 gggttctgga aatcatcaga tggttggaaa agtcgtccac atcaagatag tgttcggaga
    49261 tttaagctta aattgaagaa actaacacag aggaaatgga gtatagacct aacaagacgt
    49321 attgagcaac tgaatttgtc tattcgagga tggataaact atttctcatt gggaaatatg
    49381 aaaagtatag tcgccagcat agatgagcgc ttgcgtactc gcctacgagt gattatctgg
    49441 aagcaatgga agaagaaatc gagacgatta tggggattgc ttaagttagg ggttcctaaa
    49501 tggatagcag ataaggtatc tggctggggc gaccattatc aattagtagc tcagaagtcg
    49561 gtacttaaac gtgctatatc aaaaccagtc ctggaaaaac gtggactggt ttcgtgtttg
    49621 gattattacc ttgaacgaca tgcgttaaaa gttagttgaa ccgctgtatg ccgaacggca
    49681 cgtacggtgg tgtgagaggg gctagagatt atcccctact cgattaactc ccctgaaatt
    49741 tattttaatt atgcaaattt cacgtatttt tgatgctgag acgacgatcc tgggaacttt
    49801 tcagatattt ttttgactat ctaaatctat cattagaaaa gcttagagcg ccaaaggatt
    49861 tgagcgtttt tctgattttt aagacttttt ccagtctctt tttcgattga agatgtaatt
    49921 attctactaa ctaactaact tcttagtact agccaacaac gataatcata attcctccta
    49981 aaattaggaa taataaaggc aatagttttt gttttttcat gtaaaaaacc tcacttttgt
    50041 tttctgctat tttatgctaa aatattaaaa atcaaattta attccaaagt ttgtaactaa
    50101 agggggagcg ctacatgtct aattcatttg tcaagttgtt agtctctcaa ttatttgcaa
    50161 atttagcaga tattttcttt agagtaacaa tcattgctaa catatacatt atttcaaaat
    50221 cagtaattgc cacatcacta gttcctatct taataggaat atcctctttt gttgcgagtc
    50281 ttttagttcc gttggttact aaaaggttag cgctaaatag ggttttatct ttatctcaat
    50341 ttggaaagac tatattattg gcgatactgg taggaatgtt taccgtaatg caatccgtag
    50401 cgcctttggt gacctatcta tttgttgttg caatttccat actagatggt tttgcagcac
    50461 ctgtttccta tgctattgtg ccacgctatg cgaccgattt gggtaaggct aattcagcct
    50521 tatcaatgac tggtgaagct gttcaattga taggttgggg attaggtgga ctcttgtttg
    50581 caacaattgg tctgttacct accacgttta tcattttagt cttgtatatc atttctagct
    50641 ttctgatgtt atttcttcct aacgctgaag tggaggtgtt agagtcagaa actaatcttg
    50701 aaattttgct caaaggttgg aagttagttg ctagaaatcc tagattaaga ctttttgtat
    50761 cagcaaattt attggaaatt ttttcaaata cgatttgggt ttcttccatt atacttgttt
    50821 ttgtaacgga gttattaaat aaaacggaaa gttactgggg atattctaat acagcatact
    50881 ctattggtat tataattagt ggcttaattg cttttaggct atctgaaaag ttccttgctg
    50941 ctaaatggga aagtattctt tttcctcttg tagctatggc aatagtgaca ctgactattc
    51001 tatattttcc aaacgcacag atgtttttat tattttcagc tttagttggt atgttatcgc
    51061 aactaaaaga agttcctgaa agtgtatttc ttcaggaaac agtagaggaa aatcatttag
    51121 ttaatgtcta ttccgttctt gaagtgattt ctacattagc attttcagta tttgttttgc
    51181 taatgagcta tattactgag agttttggta ttagcatcag tttttggcta tcagccattt
    51241 gtctgatgat agaagctatt ttaatttata tcagacgaga ttattttaaa tgagagagtg
    51301 gttaaaaagt ctttaaaaat cagaaaagcg catagtatca ggtgttgaaa aaacttgata
    51361 ttgtgtgttt tattatggga agatttactt cattgtctcc tgaaatggag ttttgaagca
    51421 ggatcactct atttgaaaat atacatagaa aaactgtata atccttaaaa taagctgaaa
    51481 tctattggaa acatacggta aacaattgaa aagatactat ataaagtggt acaatggtag
    51541 aaaaataagc tagtaagaat cactcttatt ttagcgaaaa attacagaaa tgaatggttt
    51601 ttcttgatga aacagagaaa agaattgtac ctctttcttg gtcggacagc cttgtatttt
    51661 cttatctttc tagggctgct ttacttcttt agctatcttg gtcagggtca aggaagcttt
    51721 atctataatg aattttaact tgaaggagaa tccctattgt gtcaaataaa ccaatagcag
    51781 atatgattga aaccattgag cattttgctc agacacagcc tagctatcct gtctataatg
    51841 ttttggggca ggaacacact tatggcgatt taaaggctga ttcggatagt ttggctgcag
    51901 tcattgacca actaggcttg cctgagaagt ctcctgtggt tgtttttggt ggccaagaat
    51961 atgaaatgtt ggcaaccttt gtagcgctga ctaagtcagg tcatgcctac attccaattg
    52021 atagccattc ggccttggag cgagtttcag ctattttaga agtagcagag ccaagcttga
    52081 ttattgccat ttcagccttt cccttggagc aggtttctac accaatgata aatctagctc
    52141 aggttcaaga agcctttgcc caagggaata actatgaaat cacgcatcca gtcaagggag
    52201 atgataatta ctacattatc tttacttctg gtacgactgg taagcctaag ggagtgcaga
    52261 tttcacatga taatctcctc agctttacaa actggatgat tacggataag gaatttgcga
    52321 caccgagtcg tccgcaaatg ctggcacagc caccttattc ttttgacttg tctgtcatgt
    52381 attgggcacc gaccttggca cttggtggta cgcttttcac tcttccttca gtcatcactc
    52441 aggactttaa gcaactcttt gcggctatct tttcattgcc aatcgctatc tggacatcaa
    52501 caccatcctt tgcagatatg gccatgttgt ctgaatactt caacagtgag aaaatgcctg
    52561 gaatcactca tttctacttt gatggtgaag aattgacggt caaaacagct caaaaactgc
    52621 gcgagcgttt cccaaatgcc catatcatca atgcttacgg cccaacagaa gcgacagtag
    52681 ctctgtcagc agttgccgtg acagacgaga tgttagcgac tctcaaacgc ctaccaatcg
    52741 gctataccaa ggctgattct ccaaccttta tcattgacga ggaaggaaat aaactgccaa
    52801 atggtgagca gggagaaatc attgtttctg ggccagctgt ttcaaaaggt tatatgaaca
    52861 atcctgaaaa aacagcagaa gccttctttg agtttgaaaa tctgccagcc tatcacacag
    52921 gcgatgtggg aaccatgaca gatgagggct tgcttctcta cggcggacgc atggacttcc
    52981 agattaagtt caacggttac cgcattgagt tagaagatgt ctctcaaaac ctcaacaagt
    53041 ctcgctttat cgaatctgct gtcgcagtac cgcgctataa caaggaccac aaggtacaaa
    53101 atctattggc ttatgtcatc ttaaaagacg gtgttcgtga gcagtttgag cgagatatcg
    53161 atattaccaa ggccatcaag gaagacctga cagacatcat gatgtcctac atgatgccat
    53221 ctaaattcct ttaccgagac agtttgccac taactccaaa tggaaagatt gacatcaaag
    53281 gattgattaa cgaggtgaat aagagatgat ggagtttttt caacagcttc ctcatttaga
    53341 gccatatggc aatcctcagt attttgttta tgtgattgct gcaaccttgc ccatctttat
    53401 aggcctcttt ttcaagaaac gctttgcctg gtatgaagtg ttggtaagtc tcttctttat
    53461 tgtcaccatg ttggtgggtg gaaagaccaa tcaactagct gccttgggta tttacctttg
    53521 ctgggaaata ttgctcctgc ttttctacaa gcattatcga aaaagcaagg atggcaagtg
    53581 ggtcttctac ttagttagtt ttctgtccct acttccgatt atctttgtca aggtgcaacc
    53641 agctatcaat ggaacgcagt ctttgcttgg gttcttggga atttcttacc tgacctttcg
    53701 ttcggttggg attatcatcg agctgagaga tggagtgatt aaggatttta ccctctggga
    53761 attcctccgt ttccttctct tcatgccaac tttctcgagt ggtccaatcg atcgctttaa
    53821 gcgatttaat gaaaattatc agaccattcc tgagcgagat gagttgatgg atatgctgga
    53881 tgaatctgtc cgctatatca tgcggggctt tttgtataag tttatcctag ctcatgtttt
    53941 aggagagacc ttactacctc ctctgaagaa tttagccttg cagtcaggtg gcttctttaa
    54001 tctctatgcc ttggcagtta tgtatacttt tggtctggag ctcttctttg actttgcagg
    54061 ttattctatg tttgctttgg ccatctcaaa cttgatggga atccgtagcc ctatcaactt
    54121 taacaagccc tttttatcaa gggatttaaa ggagttttgg aatcgctggc atatgagtct
    54181 gtccttctgg ttccgtgact ttgtctttat gcgaatggtg atggtgttaa ccagaaagaa
    54241 agtctttaaa aatcgtaatg taacctcaag catggcctac attgtaaata tgctgattat
    54301 gggattttgg catggcgtga cctggtacta tatcgcctat ggactctttc atggactagg
    54361 tttggtcatc aatgatgcct gggttcgcaa gaaaaaaacg ctcaataagg aacggaaaaa
    54421 agcagggaag gctgccctac ctgagaatcg ctggattcag ttgcttggca tggttgtcac
    54481 tttccatgtt gtcatgttgt cattcttaat cttttctgga ttcttgaata atctatggtt
    54541 taaaaaataa aaggaaataa aatatggata tcaaatcaga agttatcgaa attattgatg
    54601 agttgtttat ggaagatgtt tctgacatga tggatgaaga tctttttgat gcaggtgtct
    54661 tggatagtat gggaacggtt gagttgattg tggagattga gaaccgtttt gacattcgtg
    54721 tccctgtaac agagtttggt cgtgacgact ggaatacagc taataaaatt atagctggta
    54781 ttgtggagct acaaaatgct taaacgctta tggatgatct tcggaccggt cttgatcgct
    54841 ggtttgttgg tttttctgct cattttcttt tatcctactg agatgcatca taatctagga
    54901 gctgaaaagc gttcagcagt ggctactact atcgatagtt ttaaggagcg aagtcaaaaa
    54961 gtcagagcac tatctgatcc aaatgtgcgt tttgttccct tctttggctc tagtgaatgg
    55021 cttcgttttg acggtgctca tcctgcggta ttagctgaga aatacaatcg ttcctaccgt
    55081 ccttatcttt taggacaggg gggagctgca tcgcttaacc aatattttgg aatgcaacag
    55141 atgttacaac agctggagaa taaacaagtt gtgtatgtta tctcacctca gtggttcagt
    55201 aaaaatggct atgatccagc agccttccag cagtatttta atggagacca gttgactagt
    55261 tttctgaaac atcaatctgg ggatcaggct agtcaatatg cagcgactcg cttactgcaa
    55321 cagttcccaa acgtagctat gaaggacctg gttcagaagt tggcaagtaa agaagaattg
    55381 tcgacagcag acaatgaaat gattgaatta ttggctcgtt ttaatgaacg ccaggcctcc
    55441 ttttttggtc agttttcggt tagaggctat gttaactacg ataagcatgt agctaagtat
    55501 ttaaaaatct tgccagacca gttttcttat caagcaatag aagatgttgt caaagcagat
    55561 gctgaaaaaa atacttccaa taatgagatg ggaatggaaa attatttcta taatgagcag
    55621 atcaagaagg atttgaagaa attaaaggat tctcagaaaa gctttaccta tctcaagtcg
    55681 ccagagtata atgacttgca gttggtttta acacagtttt ctaaatctaa ggtaaacccg
    55741 atttttatca ttccacctgt taataaaaaa tggatggact atgctggtct acgagaggat
    55801 atgtaccaac aaacggtgca gaagattcgc taccagttag aaagtcaagg ttttaccaat
    55861 atagcagatt tttctaagga cggcggggag cctttcttta tgaaggacac cattcacctt
    55921 ggttggttgg gttggttggc ttttgacaag gcagttgatc ctttcctatc caatcccaca
    55981 ccagctccga cttaccatct gaatgagcgc tttttcagca aagattgggc gacttatgat
    56041 ggagatgtca aagaatttca atagataata taatatagaa gagttcaaga atgaaactag
    56101 ttttcacgtt ttttcttgac tctttttttg cttgtgatat aattaactgg taaacaaaat
    56161 gtcaaagaat agtatttttc tcttaaaaaa gagaagtcta aaaggaaagg agtcagatat
    56221 gagacagcta gcaaaggata tcaatgcttt tttgaatgag gtgattttgc aggcggaaaa
    56281 tcagcatgaa atcctaatag gtcattgcac tagcgaggtg gccctgacca atactcagga
    56341 gcatatcctt atgctcttgt cagaggaatc tttaacaaat tcagaattgg ctcgtcgtct
    56401 caatgtcagt caggcggcag ttaccaaggc cattaagtct ttggtcaagg aagggatgtt
    56461 ggaaacatct aaagattcta aagatgcgcg tgtgattttt tatcagttga ctgacttggc
    56521 tcgtccaatc gctgaggagc accaccatca ccatgagcat acacttttaa cctatgaaca
    56581 agtggcgact cagtttactc caaatgaaca aaaagtgatt cagcggtttt tgactgcttt
    56641 agtaggagaa atcaaataat gagatatatt acggtagagg atttgtcctt ctattatgat
    56701 aaggagcctg ttcttgaaca tatcaattat tgtgttgata gtggggaatt tgttaccttg
    56761 actggggaaa atggagcggc taagacgacg ctcatcaagg ctagtcttgg aattctgcaa
    56821 ccacgcattg gaaaggtggc tatttcaaag acaaatacgc aaggtaagaa attgagaata
    56881 gcctatcttc ctcaacaaat tgccagtttt aatgctggtt ttccaagtac ggtctatgaa
    56941 tttgtcaagt cgggtcgcta tccgagaaaa ggctggttcc gtcgtttgaa tgctcatgat
    57001 gaggagcata tcaaggctag tctggactca gttggcatgt gggaacatcg agacaaacgc
    57061 ttggggtctc tatctggggg acaaaagcag cgagcggtaa ttgcgcgtat gtttgcttct
    57121 gaccctgatg tgtttatcct agacgagccg acaacgggga tggatgcagg aagtaaaaat
    57181 gaattttacg aactcatgca ccacagcgcc catcatcatg gcaaggctgt tttgatgatt
    57241 acccatgacc ctgaagaagt taaggattat gcggatcgca atattcatct agtccgtaac
    57301 caagactcgc catggcgttg tttcaatgtt catgagaatg gccaggaggt gggccatgct
    57361 tagtttatta tcttacgact ttatacagcg cgcctttttg gcggttattg ctatgagtct
    57421 tttctcaccg gtattgggaa ccttcctcat cttgcgtcgt cagagtttga tgagtgatac
    57481 ccttagccac gtctcacttt caggtgtagc ctttggtctg gttttgggga tttctccaac
    57541 tgtttctact attgccattg tcttgattgc ggcggtcttt ctggagtatc tccgtacggt
    57601 ttacaagagc tttatggaaa tcgggacagc tatcctcatg tcaacaggtc tggctgtttc
    57661 tctgattgtc atgagcaagg gtaaaagctc gagttcaatg agtttggacc aatatctctt
    57721 tggttcgatc gtgactatca gtgaagaaca ggtcatttcc ctctttgtca ttgcggtggt
    57781 tgttttgatt ttgacctttc tctttcttcg tcctatgtat atcttaactt ttgacgaaga
    57841 tacggccttt gtggatggct tgccagttcg taccatgtcc attcttttta acatggtgac
    57901 aggggtggct attgccctta tgattcctgc agcaggagct cttctggtat cgaccattat
    57961 ggtcttgcca gctagtattg ccctgcgtct ggggaaaaac tttaaatcgg ttatgctgct
    58021 tgccagtgcg attggctttt tgggaatggt agcaggactt tacatttcct actatgcaga
    58081 aacacctgca agtgcaagta ttaccattat ttttgtaact gtctttatac taatcagttt
    58141 agtaagacgt tttatcaaat aggagacgaa cgtgaaaaaa attagcttat tactagccag
    58201 tctatgtgcc ttgtttttag tggcttgttc caatcaaaaa caggcagatg gtaaactcaa
    58261 tatcgtgaca accttttacc ctgtctatga atttaccaag caagtcgcag gagatacggc
    58321 taatgtagaa ctcctaatcg gtgctgggac agaacctcat gaatacgaac catctgccaa
    58381 ggcagttgcc aaaatccaag atgcagatac cttcgtttat gaaaatgaaa acatggaaac
    58441 atgggtacct aaattgctag ataccttgga taagaaaaaa gtgaaaacca tcaaggcgac
    58501 aggcgatatg ttgctcttgc caggtggcga ggaagaagag ggagaccatg accatggaga
    58561 agaaggtcat caccatgagt ttgaccccca tgtttggtta tcaccagttc gtgccattaa
    58621 actagtagag cacatccgcg acagcttgtc agcagattat cctgataaaa aagagacctt
    58681 tgagaagaat gcagctgcct atatcgaaaa attgcaagcc ttggataagg cttacacaga
    58741 aggcttgtct caagcaaaac aaaagagctt tgtgactcaa cacgcagcct ttaactatct
    58801 tgccttggac tatggactca aacaagtcgc aatctcaggc ctttctccag atgcagagcc
    58861 atcagctgct cgcttggcag aattgacaga gtatgtcaag aaaaataaaa tcgcctatat
    58921 ctattttgaa gaaaatgcct cacaagccct tgctaacaca ctttcaaaag aagcaggtgt
    58981 caaaactgat gtcctcaatc ctttagaaag tctgacagaa gaggacacca aggctggaga
    59041 aaactacatt tccgtgatgg agaaaaacct caaggctttg aaacaaacaa cagaccaaga
    59101 aggcccagca atcgaacctg aaaaggcaga ggataccaag acagtccaaa atggttactt
    59161 cgaggatgca gctgtcaagg accgcacctt gagtgactat gcaggtaact ggcaatcagt
    59221 ttatcctttc cttgaagacg gcacgtttga ccaagtcttt gactacaagg ctaagttgac
    59281 tggtaagatg acccaggctg agtacaaggc ttactataca aaaggctatc agacagatgt
    59341 gactaagatt aacattactg ataatactat ggaatttgtt caaggtggac aaagcaagaa
    59401 atacacttac aagtatgtcg gtaagaaaat tttgacttac aagaaaggca atcgtggcgt
    59461 gcgtttcctc tttgaagcca cagatgctga cgctggacaa ttcaaatatg ttcagtttag
    59521 tgaccacaat gttgccccag ttaaggcaga acatttccat atcttttttg gaggtacaag
    59581 ccaagaagct ctatttgaag aaatggataa ttggccaacc tactacccag ataacctatc
    59641 tggccaagaa atcgcccaag aaatgttggc gcattgatga gaaatcgact agttcataag
    59701 agctagtcgg ttttttgata ctagatggct aacttcatta tagacctttc agaactaagg
    59761 gaacctgaaa aaataaataa aactcttgaa atatctggac ttatatgcta gaatgtagtt
    59821 agtgtataag gttcattaga acaccaaagc ccctaactag tgccatagtt aagggctttt
    59881 ttgacgtatc ttagcgattt aatgggtgtt tataggctag tcagtcggtc tacttcttac
    59941 catctagcca tttgcagacg aaatacaaga taattccagc tataacatct gctataatag
    60001 taagagcaag ctctggtata aggctcatta gtttcacctc ctctcacgaa cccataggaa
    60061 cgtaatcggt aaccgatgac aaaagtatta tatcacacta aatttagatc atcaacctca
    60121 cttaatgctt gaactattgt agattatatg ctaaaatgta gttaattgat aaggttcatt
    60181 agaacaccaa agcccctaac tatggccgta gttaagggct tttttgacgt atcttagcga
    60241 tttaacgggt gtttataggc tagtcactcg gtctacttct tactatctag ccatctgcag
    60301 acgaaataca agataattcc agctataaca tccgctataa tagtaagagc gagctctatg
    60361 ataaggctca ttagtttcac ctcctctcac gaacccatag gaacgtaatc ggtaaccgat
    60421 gacaaaaata gtataccaca atacatttag atcatcaagg tcacttaatt cttgaaatat
    60481 agggtttagg cgacaaagtc tttaaaatca gaaaaacgca taatatcagg tgtgcaaaaa
    60541 cttgatacta tgcgttttat tgtgggaagg tttactccat tttctcctga aattgagttt
    60601 ttgtccagcc tctgttttta gggttgctga gaaaataatg tcatgtggtg aatatttgta
    60661 aatcagtcag cagacagaac gaatactctt cgaaaatctc ttcacatcat gtcagcttcg
    60721 tctttccgta tatatgtgac tgacttcatc agttctatct acaacctcaa aacagtgttt
    60781 cgagctgact ttatcagttt tcaaatctgt actttgagca agctgagact agcttcctat
    60841 ttgattttca ttgaatatca gaaacccatt ctccatcaaa taattcgact gcgtctaata
    60901 atttttgatc tggcacggtg tctgaaataa aggttgtgta tttggagagg ggattaattt
    60961 taaaaaatcc agtcttgtaa aatttagaac tatcaatcag taagatggtt tcatgggctt
    61021 tgtcaataat attctttttt gaaatagctt ggctgagaga agcttcataa acatattggt
    61081 catcaatacc tcttgctgaa caaaatgcta aatcgatatt aaaatgatct aataaagaat
    61141 tttccttatc atagttgacc acggaacagg attgatgttt gacctcgcca gatgtgataa
    61201 agattttgga gctatcttta acagtttcag atagggtttg tgcagtatgt aaaccatttg
    61261 taaaaataat caaattatca agttcagaaa gatagggaca aagttcgtag acagtagtac
    61321 tggaatccag ataaacacac ataccagacc gaataaagtc tttagcgaga ctagcgatta
    61381 gtcttttttg cctagtactt tctccttccc gtatttgatg agaaagttca attgtgttca
    61441 tagaggacag ggtcacgtat ccgtgctttc ttttgataag accttgattt tctaagaaaa
    61501 ttaaatcacg acgtaaggta cttgtgctgg agaaagtgat ttctgccagc tcttttacgg
    61561 caattctttt tttcttttta ataatttcaa tcaattcaag tacacgttca tcttttatca
    61621 taagctcctc ctaatttatc atttcaacta tattatagca caaattggag gaatttgaat
    61681 tatttttatg aatattgggt taacatttga acattattca agtaagcgtt cacatattga
    61741 aaaaataaaa tgtggggatt ataataaagt taatcaagga cgaagagaga agaaaaatgg
    61801 aagcggtttt agcaatagat ttaggtgcga cttctggaag agcaatcgtt ggttaccttt
    61861 ctgaaaataa actagtaatg gaagaaataa atcgcttttc taatctacct attagagtaa
    61921 aagggcattt atcttgggat attgactttc tactagctaa aattcttgaa agtatccgct
    61981 tggctaatac tagttacaag attttatcta tcggtattga cacatgggga gttgattttg
    62041 gactgattga taatgaaggt aagctgttat cacaacctgt tcattatcgt gatgaaagaa
    62101 caaagggagt gttaaaggaa atatctgaaa tgactgaatt agaaaaactg tattcagaga
    62161 caggaaatca gattatggag ataaatacct tgtttcaact ctttaaggca cgtcaagaat
    62221 ctcctgactc tttctataag accaataaga ttcttttaat gccagatttg tttaattatc
    62281 tcttgacagg taagtttgct acagaaaaaa gcattgcttc aacaactcaa ttatttgatc
    62341 ctaggagtca aaattggaat cagaatatct taaaactatt tgaattggat tcatctttac
    62401 ttcctgaaat tgtttcagag ggaaatgttc ttggaaggat aaaaaaggag tatggtttag
    62461 gcgatattcc tgttgtgaat gtttgtagtc atgatacagc aagtgcgatt gtctcagtac
    62521 ctaagacaga aggtagttta tttatttcat caggtacttg gtctttggtt ggagtggaac
    62581 ttacttcacc gattcttact accgaatcct tcagttatgg atttacaaat gaagtcggta
    62641 aagatggaga gattacattt ctgaagaatt gtacagggtt gtggatcata gaggaactaa
    62701 gacgttcatt tgaacgaaga gggaaagcct attcttttga tgatattagg acaatggtgg
    62761 agaaagaaaa agaaaatctt cctctgattg atactgaatc aactgaattt gcaacagaat
    62821 ctgatatgca caagactttg acagaatatc tagcttatca tcatgaaact agagagtgga
    62881 cagatggaca actatttaag attgtttatg aaagcctagc tgaaacgtat aggaaagcga
    62941 tagagttact agaagaacta actcataagg tttataagag gatatatgtg attggaggag
    63001 gtgctagagc cagttacttt aaccaaatga ttgctgatag aactggtaaa gaggttctta
    63061 caggttcgac tgagggtaca gctgtgggga atattgttgt gcagctcata gctatgggac
    63121 aattaaaagg gatggaagaa gctcaccatg ttattgagga gtttctacaa ttagagagtt
    63181 attactccca aaagaattaa aaagattgag agtttgtaaa tttgcctccc tcccccttct
    63241 taagttttgt acaagaaggg gggataattg gtgaattgaa aaatatttag tgttttgata
    63301 tgaggaggac aagaatgtca gatgtaaaac aagaattaat taagtatggc aagaagctag
    63361 tagaaacaga tttgacgaaa ggaacaggtg ggaatctcag tgttttcgat cgtgaaaaac
    63421 aattgatggc aattaccccg tcgggtattg atttctttga aatcaaagaa tccgatattg
    63481 tagtgatgga tattaatgga aatgttgtag agggagaacg cttgccatct agcgaatggt
    63541 atatgcattt gattcaatat caaactcgtg atgatatcga tgcaattatc catgctcata
    63601 caacttatgc aacagtatta gcttgtctca gagaaccact tccggctagt cattatatga
    63661 ttgcagtggc agggaaagat gttcgggtag ctgagtatgc aacatatggt acgaaagaat
    63721 tggctgtgaa tacagctaaa gcaatggaag gtcgtagagc agttttacta gcgaatcatg
    63781 gaattttagc aggtgcacaa aatttattga atgcatttaa tattgttgaa gaagttgaat
    63841 attgtgcaaa aatttattgt ttagctaaga attttggaga gccagtagtt cttcctgatg
    63901 aggagatgga tttaatggca gaaaaattta aaacatacgg tcagagaaaa tagggaggat
    63961 attaatgtta aaacatatac cgaaaaatat ttctccagat ttattgaaga ctttaatgga
    64021 aatgggacat ggagatgaaa tagtattagc tgacgcgaat tatccttctg cctcatgtgc
    64081 aaataagcta attcgttgtg atggcgtaaa tattccagaa ttattagatt ccattctgta
    64141 tttaatgcca ttagatagtt acgtcgatag ttcaattcag tttatgaacg ttgtttcggg
    64201 tgatgatatt cctaagatat ggggtaccta tagacagatg attgaaggtc atggtacaga
    64261 tcttaaaacg attacttatc ttagaagaga agacttttat gaacgtagta agaaagctta
    64321 tgctattgtt gctacaggag aaacttcact ttatgctaat attatcctta agaaaggagt
    64381 agttgttgaa agagaaaatg tttaatagag gaattttagt tgccagtcat ggtaattttg
    64441 ctagcggagc tctcatgacc gcagaaatgt ttgttggtga gacaacaaat gatagagtta
    64501 ggacattagg tttgatgcct ggagagaata ttgtagagtt tgaacattat tttaaaaatc
    64561 aagtaaatga actgttagac tcaaatcaag aggttatcgt tttgactgac ttgattggag
    64621 gaagtcctaa taatgtggct ttgtcacggt ttttaaattt ggattcagtt gatattgtaa
    64681 cagggtttaa tatccctctc ctagtggaat taatatcaag ttatgattca aaaatcaatt
    64741 tagaagaaat tgttcaaaat gctcaaaata gtttgtttaa tgttaaacaa caacttaacg
    64801 tagaggagga agaggattta tgtctataga gtttgttcgt attgatgacc gtctggtaca
    64861 tggtcaagtt gtcactacgt ggctaaaaaa gtatgatatt gagcaagtta tcattgttaa
    64921 tgatcgcatc tcagaagata aaacacgaca atctatttta aagatttctg caccggtagg
    64981 tttaaaaatt gttttcttta gtgtaaaacg gtttgtggaa gttttaaact ctgtgccaat
    65041 aaaaaagaga acaatgctga tatatacaaa tccaaaagat gtgtatgatt ctattgaagg
    65101 aaatttaaaa ttggagtacc tcaatgtagg acagatgagt aaaacggagg aaaatgaaaa
    65161 ggtaacggga ggtgtagctc taggtgaaga agacaaatat tattttaaga a
//
LOCUS       DAVLFB010000003        49383 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLFB010000003 DAVLFB010000000
VERSION     DAVLFB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.003"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            434..1909
                     /locus_tag="VXD58_000148"
     CDS             434..1909
                     /locus_tag="VXD58_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV0674913.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1875..2590)
                     /locus_tag="VXD58_000149"
                     /pseudo
     CDS             complement(1875..2590)
                     /locus_tag="VXD58_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2846..3706
                     /gene="speE"
                     /locus_tag="VXD58_000150"
     CDS             2846..3706
                     /gene="speE"
                     /locus_tag="VXD58_000150"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV0674914.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            3703..4962
                     /locus_tag="VXD58_000151"
     CDS             3703..4962
                     /locus_tag="VXD58_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV0674915.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4962..6089
                     /gene="nspC"
                     /locus_tag="VXD58_000152"
     CDS             4962..6089
                     /gene="nspC"
                     /locus_tag="VXD58_000152"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV0674916.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6086..7171
                     /gene="aguA"
                     /locus_tag="VXD58_000153"
     CDS             6086..7171
                     /gene="aguA"
                     /locus_tag="VXD58_000153"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV0674917.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7181..8056
                     /gene="aguB"
                     /locus_tag="VXD58_000154"
     CDS             7181..8056
                     /gene="aguB"
                     /locus_tag="VXD58_000154"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV0674918.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8237..9046)
                     /locus_tag="VXD58_000155"
     CDS             complement(8237..9046)
                     /locus_tag="VXD58_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0674919.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9318..9539
                     /locus_tag="VXD58_000156"
     CDS             9318..9539
                     /locus_tag="VXD58_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV0674920.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            9593..10264
                     /locus_tag="VXD58_000157"
     CDS             9593..10264
                     /locus_tag="VXD58_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0674921.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10316..10513)
                     /locus_tag="VXD58_000158"
     CDS             complement(10316..10513)
                     /locus_tag="VXD58_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0674922.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            10506..11414
                     /locus_tag="VXD58_000159"
     CDS             10506..11414
                     /locus_tag="VXD58_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0674923.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11411..11872
                     /locus_tag="VXD58_000160"
     CDS             11411..11872
                     /locus_tag="VXD58_000160"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV0674924.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            11862..12749
                     /locus_tag="VXD58_000161"
     CDS             11862..12749
                     /locus_tag="VXD58_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0674925.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12752..14635
                     /gene="cbpE"
                     /locus_tag="VXD58_000162"
                     /gene_synonym="pce"
     CDS             12752..14635
                     /gene="cbpE"
                     /locus_tag="VXD58_000162"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV0674926.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14715..15845
                     /locus_tag="VXD58_000163"
     CDS             14715..15845
                     /locus_tag="VXD58_000163"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV0674927.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            15855..17117
                     /locus_tag="VXD58_000164"
     CDS             15855..17117
                     /locus_tag="VXD58_000164"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV0674928.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            17121..17918
                     /gene="proC"
                     /locus_tag="VXD58_000165"
     CDS             17121..17918
                     /gene="proC"
                     /locus_tag="VXD58_000165"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV0674929.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            18034..18672
                     /locus_tag="VXD58_000166"
     CDS             18034..18672
                     /locus_tag="VXD58_000166"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV0674930.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTNAVLFDGMGLAK"
     gene            18669..19559
                     /locus_tag="VXD58_000167"
     CDS             18669..19559
                     /locus_tag="VXD58_000167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV0674931.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLAHQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19599..19916
                     /gene="yabA"
                     /locus_tag="VXD58_000168"
     CDS             19599..19916
                     /gene="yabA"
                     /locus_tag="VXD58_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV0674932.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            19919..20788
                     /gene="rsmI"
                     /locus_tag="VXD58_000169"
     CDS             19919..20788
                     /gene="rsmI"
                     /locus_tag="VXD58_000169"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV0674933.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            21015..21533
                     /locus_tag="VXD58_000170"
     CDS             21015..21533
                     /locus_tag="VXD58_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV0674934.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21642..22290
                     /locus_tag="VXD58_000171"
                     /pseudo
     CDS             21642..22290
                     /locus_tag="VXD58_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(22279..23082)
                     /locus_tag="VXD58_000172"
                     /pseudo
     CDS             complement(22279..23082)
                     /locus_tag="VXD58_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            23077..23331
                     /locus_tag="VXD58_000173"
     CDS             23077..23331
                     /locus_tag="VXD58_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0674935.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            23577..24911
                     /gene="trmFO"
                     /locus_tag="VXD58_000174"
     CDS             23577..24911
                     /gene="trmFO"
                     /locus_tag="VXD58_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV0674936.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            24995..25732
                     /locus_tag="VXD58_000175"
     CDS             24995..25732
                     /locus_tag="VXD58_000175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV0674937.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            25741..26298
                     /gene="frr"
                     /locus_tag="VXD58_000176"
     CDS             25741..26298
                     /gene="frr"
                     /locus_tag="VXD58_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV0674938.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            26358..27212
                     /gene="cvfB"
                     /locus_tag="VXD58_000177"
     CDS             26358..27212
                     /gene="cvfB"
                     /locus_tag="VXD58_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV0674939.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            27221..27436
                     /locus_tag="VXD58_000178"
     CDS             27221..27436
                     /locus_tag="VXD58_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV0674940.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            27522..28490
                     /locus_tag="VXD58_000179"
     CDS             27522..28490
                     /locus_tag="VXD58_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV0674941.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(28468..28647)
                     /locus_tag="VXD58_000180"
     CDS             complement(28468..28647)
                     /locus_tag="VXD58_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674942.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            28683..29183
                     /locus_tag="VXD58_000181"
     CDS             28683..29183
                     /locus_tag="VXD58_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0674943.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            29186..29446
                     /locus_tag="VXD58_000182"
                     /pseudo
     CDS             29186..29446
                     /locus_tag="VXD58_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(29813..30139)
                     /locus_tag="VXD58_000183"
     CDS             complement(29813..30139)
                     /locus_tag="VXD58_000183"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674944.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(30085..30498)
                     /locus_tag="VXD58_000184"
     CDS             complement(30085..30498)
                     /locus_tag="VXD58_000184"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV0674945.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(30499..30924)
                     /locus_tag="VXD58_000185"
     CDS             complement(30499..30924)
                     /locus_tag="VXD58_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV0674946.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            31101..31670
                     /locus_tag="VXD58_000186"
     CDS             31101..31670
                     /locus_tag="VXD58_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0674947.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            31738..32388
                     /locus_tag="VXD58_000187"
     CDS             31738..32388
                     /locus_tag="VXD58_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV0674948.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            32372..34612
                     /locus_tag="VXD58_000188"
     CDS             32372..34612
                     /locus_tag="VXD58_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV0674949.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            34754..34978
                     /locus_tag="VXD58_000189"
     CDS             34754..34978
                     /locus_tag="VXD58_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674950.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYDSYSEYPVGSGWNYGRYEIINYYSGGY"
     gene            35011..35598
                     /locus_tag="VXD58_000190"
     CDS             35011..35598
                     /locus_tag="VXD58_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0674951.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            35602..36783
                     /locus_tag="VXD58_000191"
     CDS             35602..36783
                     /locus_tag="VXD58_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0674952.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    36943..37074
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            37090..37620
                     /gene="infC"
                     /locus_tag="VXD58_000192"
     CDS             37090..37620
                     /gene="infC"
                     /locus_tag="VXD58_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV0674953.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            37653..37853
                     /gene="rpmI"
                     /locus_tag="VXD58_000193"
     CDS             37653..37853
                     /gene="rpmI"
                     /locus_tag="VXD58_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV0674954.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            37905..38264
                     /gene="rplT"
                     /locus_tag="VXD58_000194"
     CDS             37905..38264
                     /gene="rplT"
                     /locus_tag="VXD58_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV0674955.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            38322..38702
                     /locus_tag="VXD58_000195"
     CDS             38322..38702
                     /locus_tag="VXD58_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0674956.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            38952..39752
                     /locus_tag="VXD58_000196"
     CDS             38952..39752
                     /locus_tag="VXD58_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV0674957.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            39763..40701
                     /locus_tag="VXD58_000197"
     CDS             39763..40701
                     /locus_tag="VXD58_000197"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV0674958.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            40743..42644
                     /locus_tag="VXD58_000198"
     CDS             40743..42644
                     /locus_tag="VXD58_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV0674959.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(42705..44360)
                     /gene="pavA"
                     /locus_tag="VXD58_000199"
     CDS             complement(42705..44360)
                     /gene="pavA"
                     /locus_tag="VXD58_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV0674960.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIGLDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            44465..44962
                     /gene="ybeY"
                     /locus_tag="VXD58_000200"
     CDS             44465..44962
                     /gene="ybeY"
                     /locus_tag="VXD58_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV0674961.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHKLNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            44943..45338
                     /locus_tag="VXD58_000201"
     CDS             44943..45338
                     /locus_tag="VXD58_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV0674962.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            45355..46254
                     /gene="era"
                     /locus_tag="VXD58_000202"
     CDS             45355..46254
                     /gene="era"
                     /locus_tag="VXD58_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV0674963.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            46303..47127
                     /gene="mutM"
                     /locus_tag="VXD58_000203"
     CDS             46303..47127
                     /gene="mutM"
                     /locus_tag="VXD58_000203"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV0674964.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            47127..47732
                     /locus_tag="VXD58_000204"
     CDS             47127..47732
                     /locus_tag="VXD58_000204"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV0674965.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            47719..48918
                     /locus_tag="VXD58_000205"
     CDS             47719..48918
                     /locus_tag="VXD58_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV0674966.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            48918..49067
                     /gene="rpmG"
                     /locus_tag="VXD58_000206"
     CDS             48918..49067
                     /gene="rpmG"
                     /locus_tag="VXD58_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0674967.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            49107..49340
                     /gene="secG"
                     /locus_tag="VXD58_000207"
     CDS             49107..49340
                     /gene="secG"
                     /locus_tag="VXD58_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV0674968.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
BASE COUNT        14650 a         9007 c        11257 g        14469 t
ORIGIN      
        1 aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa aatgagatga
       61 tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag ggttgaatgc
      121 cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt tataagttga
      181 tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca cgtcatcttg
      241 tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac atcgggaaca
      301 gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag gtgaaaacct
      361 gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct aaaaatattg
      421 aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg aggccttggt
      481 gaagttacgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc gtggacgggg
      541 aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg tcaattcgat
      601 gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag aggagctggc
      661 tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa cttcatcggt
      721 gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc cacgaaatgt
      781 ccataaatct gctatcaatg cgttggttct atgtggtgcc attcccatct atatcgagat
      841 gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag tagcacaggc
      901 cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt actacggcat
      961 ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga tggttttagt
     1021 agatgaagcc catggagcgc atttgcattt cactggtaaa cttccaattt ctgctatgga
     1081 tgcaggagct gatatggcag cagtttccat gcataagtct ggtgggagtt tgacacaaag
     1141 ttcgcttctt ttaatcgggg agcagatgaa ttctgaatac gttcgtcaga taattaacct
     1201 gactcagtct acatctgcct cttacttgtt gatggctagt ttggatattt cacgtcgcaa
     1261 cttggccctt cgtggtaaag agtcgtttga gaaagtcatt gagctatctg agtatgcccg
     1321 ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa tagacggtgt
     1381 ttcggtttgc gattttgacg taactaagct gtcagtttac actcagggta ttggcttaac
     1441 aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg agtttggtga
     1501 tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca tcgagcgctt
     1561 ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag atttgatagc
     1621 aggagaatat attcagcccg agttggtgct gtctccacaa gaagccttct attcagagag
     1681 aaaaagtttg accttagatg agtctgttgg acaggtctgt ggagaatttg tcatgtgcta
     1741 tcctccaggg attcctatct tggctcctgg tgaacgcatt acacgagaaa ttgtagacta
     1801 tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc cagaggtcaa
     1861 tcatatcaac gttattaaga gaaagacaaa ctataagaaa agtcaatagt tttatctaaa
     1921 ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca aaaagccctt
     1981 gaattagaag cggtcaatcg cttaatttct atcagcttat caaatcctgc ctcaatcctt
     2041 ttctgaggat tagggtagcg tgtcaagagt tggtaggtat attctgaatg ctttccaacg
     2101 attttatcca actcaggaaa gatgatatca agacaacgag tgtattgtac tttccaatca
     2161 gactgttttt cttgagacga tgaatatgtc tagccagtat ttttagttct acttgccgat
     2221 tatcgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg atgccatgag
     2281 cgtctttctt atccgtttta gtcttgtgaa gtgataatga tttggcaaat tccttgatga
     2341 gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc agtagattaa
     2401 aggcataatg tccggtattt tcaagagcga tgagacagtc ttggttgagt tgtcgaagag
     2461 acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt ttaagaacag
     2521 tttttcctgg aacattcaag gctgtaacat cgtgtttatt tttagcgaca tcaatgccca
     2581 cataaagcat gggagtacct ccagatgtag tatttcaagt ctacttggtt atccacgaat
     2641 tttttgcctt gttaccttag acgagataaa acgtctatgc gttatcaaac tcattaccaa
     2701 ttgaaacaaa aaactgtggt tagagccttt cggaaatcgt caagcgattg gagggaataa
     2761 actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta gctaactgcg
     2821 ctaaatataa tataaggaga aatagatgga tttatggttt tctgaagttc atactccaga
     2881 tgtcaaattg tctctgagaa cagccaagca actttacgct ggaaaaagtg aatggcagga
     2941 tatcgaagtc ttggatacgc cagcttttgg gaaaatactg attttaaatg gccatgtctt
     3001 gttctcagat gcggatgatt tcgtctacaa tgaaatgacc gttcacgttc ccatggctgt
     3061 ccacccaaat ccaaagaaag tattggttat tgggggtggt gacggcggtg ttgcccaagt
     3121 attaacactc tatcctgaac tggaacaaat cgatatcgtg gaaccggatg agatgttggt
     3181 cgaggtctgt cgtgagtatt tcccagactt tgctgcaggg ctagatgatc ctcgtgttac
     3241 catttactac caaaatgggc tacgcttttt gcgaaactgc gaagatgatt acgatattat
     3301 catcaacgat gcgacagatc catttggcca tacggaagga ctctttacca aggaattcta
     3361 cggcaatagt tatcgagctc tgaaggaaga cggcatcatg atttaccagc atgggagtcc
     3421 cttctttgac gaggatgagt cggcctgccg aagcatgcac cgcaaggtca atcaagcctt
     3481 tccaatcagt cgggtttatc aggcccatat tccaactagc ccagctggct attggttgtt
     3541 tggatttgca tcgaaaaaat accaccctgt caaagatttt gacaaggaag gttggaaaaa
     3601 acgccagctt ttcacagaat actacactgc aaacttacac gtgggatcct ttatgttgcc
     3661 caagtatgtt gaggacattt tagaagaaga ggaaggaaaa aaatgagtcg tttactagtt
     3721 attggttgtg ggggcgttgc ccaagttgct atttcaaaga tttgtcaaga tagcgaaaca
     3781 tttacagaga ttatgattgc tagccgtacc aagtcaaaat gcgatgactt gaaagcgaag
     3841 ctagaaggca aaacaagtac taaaattgaa actgcagcac ttgatgctga caaggttgaa
     3901 gaagtgattg ccctgattga aagctacaaa ccagaagctg ttttgaatgt agctctgcct
     3961 tatcaagatt taaccattat ggatgcttgt ttggcaacag gtgttcacta tatcgataca
     4021 gccaactacg aagcagaaga cacagaagac cctgagtggc gtgctatcta cgaaaaacgt
     4081 tgtaaggaac ttggttttac agcctacttt gactactcat ggcagtgggc ttatcaagag
     4141 aaattcaaag aagcaggctt gactgctctt cttggttctg gttttgaccc aggtgtaact
     4201 agtgtctttt cagcttatgc cctcaaacac tattttgatg aaatccatta tatcgacatt
     4261 ttagactgta atggcggtga ccacggttat ccatttgcaa ccaactttaa tccagaaatt
     4321 aatctccgtg aggtttctgc gccaggttct tactgggaag atgggaaatg ggtcgaagtc
     4381 gaagctatgt ctatcaagcg tgagtatgat ttccctcaag ttggacaaaa agatatgtat
     4441 ctccttcacc atgaagaaat cgaatcattg gccaagaaca ttccaggtgt caaacgcatt
     4501 cgtttcttta tgacttttgg tcaatcttac ttgacgcaca tgaaatgtct tgaaaatgtt
     4561 ggactccttc gtacggatac cattaacttt aacggccaag aaattgttcc aattcaattt
     4621 ttgaaagcct tgcttccaga tcctgctagc cttggcccac gtactgtagg taaaaccaat
     4681 attggatgta tctttacagg tgtcaaagat ggcgttgaaa agactatcta catctacaat
     4741 gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac aagcaatttc atacacgaca
     4801 ggcgttccag ccatgattgg gacaaaatta gtcatgaacg gaacttggaa acaagctgga
     4861 gtgtataacc ttgaggagtt agatccagat ccattcatgg aagctttgaa tgagtatggt
     4921 ttgccatggg ttgtggttga aaatccacaa atggtggact aatgaagtta gaacaagtac
     4981 caacaccagc ctatgttatt gacttggcca agttagaagc taattgccgc attctacaat
     5041 atgtacaaga agaggccggt tgcaaggtct tgcttgccca gaaggcatat tccctctaca
     5101 aaacttatcc cttgattagc cagtatctat caggtacgac agctagtgga ctctatgagg
     5161 ccaaattggc aagggaagaa tttcctggtg aagtccatgt ttttgcacca gctttcaagg
     5221 atgaggacat ggaggaattg cttgggataa cagaccatat cgtctttaac tcagagagac
     5281 agttgcgtaa gcatggggct cgttgtcgag aggctggtgt cagtgttggt ttgcgcctca
     5341 accctcagtg ttcaactcaa ggagatcacg cgctctatga cccttgtgca cctggttctc
     5401 gctttggagt tactatagac aagattccga gtgatttgct agatttggtt gacggacttc
     5461 attttcatac cctttgcgag cagggagcag atgatttaca aacaactttg aaagcagtag
     5521 aagaacagtt tggtccctac ttacatgagg taaaatggct caatatgggt ggtggtcatc
     5581 atattacaag agaaggttac gatgtggatt tgctgatttc agaaatcaag cgtatccgaa
     5641 aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc cattgcgctt aatgcgggtt
     5701 atttagcaac tgaggtatta gatattgtag aaaacggtat ggaaatcttg gttttagacg
     5761 cctctgcgac ctgccatatg cctgatgtac ttgagatgcc ctatcgtcca cctttgagaa
     5821 atggctttga gtcacaggaa aaagcccata cctacagact ttcttctaat acctgtctga
     5881 ctggcgatgt gattggtgat tatagttttg aaaatccagt ccaaatcgga gacagacttt
     5941 attttcaaga catggccatt tattcttttg tcaaaaataa tacctttaat ggtattggat
     6001 tgccaagtct ctatctcatg gacgaacagg gagactgtag cttactcaaa gcttttggct
     6061 atcaagactt taaagggaga ttatcatgat ggacagtcca aaaaaattag gctatcacat
     6121 gccagcagag tacgaacccc atcatggtac cctcatgata tggccgactc gaccaggatc
     6181 atggcctttt caaggaaagg ctgctaaaag agcatttact cagatcatcg agaccatagc
     6241 agaaggggaa agagtctatc ttttggtgga gcaggcctat ctatctgaag cccaatccta
     6301 tcttggagac aaggttgttt atttagacat tcccaccaat gatgcctggg cgcgtgatac
     6361 tggcccaacc attctcgtca atgataaagg taagaaatta gccgtggatt gggcctttaa
     6421 tgcttgggga ggcacctatg atggtcttta tcaagattat gaagaggatg accaagtagc
     6481 cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat gctaaacctt ttgtactgga
     6541 aggaggcgca atccatagcg atggtcaagg aactattctc gtaactgaaa gttgcttgct
     6601 tagtcctggt cgcaatccta acctgactaa agaggagatt gaaaacacat tattagaaag
     6661 tcttggtgct gaaaaagtta tttggcttcc ttatggtatt tatcaggatg aaaccaatga
     6721 acacgtcgat aatgttgttg cctttgttgg tcctgctgag gttgttttgg cttggacaga
     6781 tgacgaaaat gatccccagt atgccatgtc aaaagcagat ctcgaactct tagaacagga
     6841 aacagatgca aaaggttgtc acttcaccat tcataaattg cctatccctg cagttcgaca
     6901 agttgtgaca gaagaagatt tgccaggcta catctatgaa gaaggagaag aagagcgata
     6961 cgcaggtgaa cgactagcag cttcctacgt aaacttttat atcgccaaca aggctgtctt
     7021 ggttccacag tttgaggatg taaacgacca agtggcctta gatatcctca gcaagtgttt
     7081 cccagaccgt aaagttgtcg gaataccagc cagagatatt ctcttaggtg gtggcaatat
     7141 ccactgtatc acccaacaaa ttccagaata ggagaaaaag atgagaaatg taagagttgc
     7201 aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat atccaaaccg cagagcgttt
     7261 agtacgtcag gctgctgagc aaggagccca aattattctc ttgcccgagt tgtttgaaca
     7321 tccctatttc tgtcaggaac gtcagtatga ctactaccag tatgcccaat ctgtagcgga
     7381 aaatactgcc attcagcatt ttaaggtgat tgctaaggaa ctacaagttg ttttaccaat
     7441 cagtttctat gaaaaagatg gtaatgtctt gtataactct attgccgtca ttgatgcaga
     7501 tgggggagtg ctgggcgttt atcgaaagac ccatatacca gatgaccatt attatcaaga
     7561 aaaattctat ttcacgcctg gtaacactgg tttcaaggtc tggaatactc gctatgctaa
     7621 gattggtatc ggtatctgtt gggatcaatg gttccctgaa acagcccgct gtcttgcatt
     7681 gaatggtgct gaattgctct tttatcctac agctatcggt tcagagccaa ttttggatac
     7741 agatagttgt ggtcactggc aacgtactat gcaagggcac gcagcagcga atattgttcc
     7801 agtcatcgca gccaatcgtt atggtttaga ggaggttact cctagtgagg aaaatggcgg
     7861 acagagttcc agtcttgact tctacggttc ctcctttatg acggatgaaa caggagctat
     7921 tctagaacga gctgaaagac aagaagaagc tgttctgtta gctacttatg acctagacaa
     7981 gggagcaagt gaacgtctaa actggggctt gtttcgagat agaagaccag aaatgtatag
     8041 acaaattaca gattagtgtg ggagaaatga gagattcatt ctgctagact aacttcttat
     8101 tagcaactat aagatactat ggcatctagt aaatcgattt ttatgattcg ctattcttgt
     8161 ctattgatta gtccgtattt taaaatatta gcaaaaaagc aaatagcagt aacttctgtc
     8221 tatttgcttt tcttttttat agaatatatt tctcaatagc acgcgcaacg ccgtcttctt
     8281 cgttgcttga ggtaacggca tccgcaagag atttgacata atcgctggca tttcccattg
     8341 caatcccaag ccctgcaaac tggagcattt cgatatcgtt attagcatcg cccatggcca
     8401 taatctctga ggaatcaatc ttcaaaatct cagctagtcg tgaaagagca gtagcctttg
     8461 tcgttccaag cggcattgct tcataaatga caggctgcga acgaactcca ctgaatcgtt
     8521 ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt ttgttctttt gttcctaaaa
     8581 acataccttg gaacatccgg aactttccac tagtcgcttc ttcaagagaa atttcagtca
     8641 ggtctgaaaa tactagttta gcatcatttt caataacttg attgggtttg ccaccgagaa
     8701 caaaataatg tgactcgtca aaaagtgtca actgaacatc actcttttca gcaaggtcat
     8761 agaggtattc gatgtcagct ggactcagtt ctttccagtc aactagactc caatcactgg
     8821 tctggtgagt tgaacaaccg ttgttaacaa taatatattc gttctggagg tcaagctcca
     8881 gttttttgta gtaggggagg acaccgaaaa gggggcgacc cgtacagaga accagtttga
     8941 cacctttttc aatggctttg tgaatagcag taatgtgagc ttgtgggatt tccttggctt
     9001 cattgaggag ggtgccgtcc atatccaagg ctagtagttt aatcataggt cttccttctt
     9061 tatcttttgc tattattata gcatattttg gagaagaaat tgatagaaag cttgagacta
     9121 attgatttta tagtttaaga tgttttgatg acaattcatg atttgaagag gatatttggc
     9181 aaagatatgc tatactatgt ttgtcaatgt tgcaactaga caaattaaaa aaccaactta
     9241 atataatagt ttttttgtaa gtaggtatga gtatcagatt actcaactaa tctgaagaat
     9301 aatggaggaa atatatcatg attttaatga caaaaaatat aaatctaaca aatgaagaat
     9361 tagagctgat acaaggtgga gcagattcat atggtaaaaa tcctaatggt aggtacgatt
     9421 gggaaataga accagtatta actctgccgg ttcatggatt ttgttccaga ggcacctatg
     9481 atttaggata tattggagga ggcaatcatc tttgcaaagg aagtgctgcg agattttaag
     9541 taaaatttat tagtaatatg aagaaacaag tggagaaagc agagaattta atatgaaaaa
     9601 acgggctatt caaattttac tagcattgtc cttaattttt tacaaatcaa cttggttttg
     9661 gagagttttc aatcacctcg caaagcccta tctaccagca agtcgtgaat tttttcagat
     9721 tctgcttttg atggagagcg gagttctttt cttagcggtc atctatctac tggtttttgc
     9781 aggaaagaaa atttttcatt tcaagtggca gctgaggtac ttcatctacc ttttactggg
     9841 ctacatcatt tcatatatgt ctgacttcct cttttcgtat ttcatatccc tgtcttcaaa
     9901 tcagatttct ttgaatgaaa cgatagaaat gatggggaga caggagttcc cttatttctt
     9961 gctcatcgtt tgcttcatcg cccctattgc tgaggaattg atttatcgag gtgtgcttat
    10021 gacaaccttt ttcaaaaact caccttggta cggagatgtt ttgctttctg ctattatttt
    10081 cggttatatt catatcaatt ttgctttaac tcctcttgct tttttcattt atgctagtgg
    10141 aggtctgatt ttagctctat tgtatcgcat gactaaaaat ctctactatc caatactagt
    10201 tcatattctc atcaatatca ctgccttctg ggatgtttgg ttactcctat tttcaggaag
    10261 ttagcttact aaaaaaatgt cggaattttc cggcattttc ttttttcaca aatagtcaac
    10321 gtttttcttt ccgatactga agtggtgtgt agccacttat ttttttgaat tgattttgaa
    10381 aataagattg gcgtgagaaa ggcagatagt gaagatagtt aagaagaata ggatgttctt
    10441 ttttcctttt tggaaaactt ctaaaatatg gtataatgaa aagataaaga agttgggggt
    10501 agaagatgaa cattcaacaa ttacgctatg ttgtggctat tgccaatagt ggtacttttc
    10561 gtgaagctgc tgaaaagatg tatgttagtc agccgagtct gtctatttct gttcgtgatt
    10621 tggaaaaaga gttgggcttt aagattttcc gtcggaccag ctcagggact ttcttgaccc
    10681 gtcgtgggat ggaattttat gaaaaagcgc aagaattggt taaaggattt gatatttttc
    10741 aaaatcagta tgccaatcct gaagaagaaa aagatgaatt ttccgttgct agccagcact
    10801 atgacttctt accaccaact attacggcct tttcagagcg ctatcctgac tataagaact
    10861 tccgtatttt tgaatcaact actgttcaaa tattagatga agtggcgcaa gggcatagtg
    10921 agattgggat tatctacctc aacaatcaaa ataaaaaggg gattatgcaa cgggttgaaa
    10981 agttaggtct ggaggtcatc gaattgattc ctttccatac ccatatttat ctctgtgagg
    11041 gtcatccttt agcccagaaa gaggaattag tcatggagga tttagcggat ttaccaacgg
    11101 ttcgtttcac tcaagagaaa gacgagtacc tttattattc agagaacttt gtcgatacca
    11161 gcgctagctc acagatgttt aatgtgacag accgtgccac cttgaatggt attttggagc
    11221 ggacggacgc ctatgcgaca ggttctggat ttttagatag tgacagtgtt aatggcatta
    11281 cagttattcg tctcaaggat aacctagata accgcatggt ctatgttaaa cgtgaagaag
    11341 tggagcttag tcaagctggg actctcttcg tagaagtcat gcaagaatat tttgatcaaa
    11401 agaggaaatc atgaaaaaaa gagcaatagt ggcagtcatt gtactgcttt taattgggct
    11461 ggatcagttg gtcaaatctt atatcgtcca gcagattcca ctgggtgaag tgcgctcctg
    11521 gatccccaat ttcgttagct tgacctacct gcaaaatcga ggtgcagcct tttctatctt
    11581 acaagatcag cagttgttat tcgctgtcat tactctggtt gtcgtgatag gtgccatttg
    11641 gtatttacat aaacacatgg aggactcatt ctggatggtc ttgggtttga ctctaataat
    11701 cgcgggtggt cttggaaact ttattgacag ggtcagtcag ggctttgttg tggatatgtt
    11761 ccaccttgac tttatcaact ttgcaatttt caatgtggca gatagctatc tgacggttgg
    11821 agtgattatt ttattgattg caatgctaaa agaggaaata aatggaaatt aaaattgaaa
    11881 ctggtggtct gcgtttggat aaggctttgt cagatttgtc agaattatca cgtagtctcg
    11941 cgaatgaaca aattaaatca ggccaggtct tggtcaatgg tcaagtcaag aaagctaaat
    12001 acacagtcca agagggtgat gtcgtcactt accatgtgcc agaaccagag gtattagagt
    12061 atgtggctga ggatcttccg ctagaaatag tctaccaaga tgaggatgtg gctgtcgtta
    12121 acaaacctca gggaatggtt gtgcacccga gtgctggtca taccagtgga accctagtaa
    12181 atgccctcat gtatcatatt aaggacttgt cgggtatcaa tggggttctg cgtccaggga
    12241 ttgttcaccg tattgataag gatacgtcag gtcttctcat gattgctaaa aacgatgatg
    12301 cgcatctagt acttgcccaa gaactcaaag ataaaaagtc tctccgcaaa tattgggcga
    12361 ttgttcatgg aaatctgcct aatgatcgtg gtgtaattga agcgccgatt ggccggagtg
    12421 aaaaagaccg taagaaacag gctgtaactg ctaaagggaa gcctgcagtg acgcgttttc
    12481 acgtcttgga acgctttggc gattatagct tagtagagtt gcaactggag acagggcgca
    12541 ctcatcaaat ccgtgtccac atggcttata tcggccattc agtcgctggt gatgaggtct
    12601 atggtcctcg caagactttg aaaggacatg gacaatttct tcatgccaag actttaggtt
    12661 ttactcatcc gagaacaggt aagaccttgg aatttaaagc agatatccca gagattttta
    12721 aggaaacctt ggagagattg agaaagtaag aatgaaaaag aaattaacta gtttagcact
    12781 tgtaggcgct tttttaggtt tgtcatggta tgggaatgtt caggctcaag aaagttcagg
    12841 aaataaaatc cactttatca atgttcaaga aggtggcagt gatgcgatta ttcttgaaag
    12901 caatggacat tttgccatgg tggatacagg agaagattat gatttcccag atggaagtga
    12961 ttctcgctat ccatggagag aaggaattga aacgtcttat aagcatgttc taacagaccg
    13021 tgtctttcgt cgtttgaagg aattgggtgt ccaaaaactt gattttattt tggtgaccca
    13081 tacccacagt gatcatattg gaaatgttga tgaattactg tctacctatc cagttgaccg
    13141 agtctatctt aagaaatata gtgatagtcg tattactaat tctgaacgtc tatgggataa
    13201 tctgtatggc tatgataagg ttttacagac tgctgcagaa aaaggtgttt cagttattca
    13261 aaatatcaca caaggggatg ctcattttca gtttggggac atggatattc agctctataa
    13321 ttatgaaaat gaaactgatt catcgggtga attaaagaaa atttgggatg acaattccaa
    13381 ttccttgatt agcgtggtga aagtcaatgg caagaaaatt taccttgggg gcgatttaga
    13441 taatgttcat ggagcagaag acaagtatgg tcctctcatt ggaaaagttg atttgatgaa
    13501 gtttaatcat caccatgata ccaacaaatc aaataccaag gatttcatta aaaatttgag
    13561 tccgagtttg attgttcaaa cttcggatag tctaccttgg aaaaatggtg ttgatagtga
    13621 gtatgttaat tggctcaaag aacgaggaat tgagagaatc aatgcagcca gcaaagacta
    13681 tgatgcaaca gtttttgata ttcgacaaga cggtttagta aacatttcaa cttcctacaa
    13741 gccgattcca agttttcaag ctggttggca taagagttca tatggaaact ggtggtatca
    13801 agcgcctgat tctacaggag aatatgctgt cggttggaat gaaattaagg gtgaatggta
    13861 ttactttaac caaacgggta tcttattaca gaatcaatgg aaaaaatgga acaatcgttg
    13921 gttctatttg acagactctg gtgttgctgc taaaaattgg aagaaaatcg ctggaatctg
    13981 gtattatttt aacaaagaaa accagatgga aattggttgg attcaagata aagagcagtg
    14041 gtattatttg gatgttgatg gctctatgaa gacaggttgg cttcaatata aggggcaatg
    14101 gtattacttt gctccatcag gggaaatgaa aacgggctgg gtaaaagata aagaagcctg
    14161 gtactatatg gattctactg gtatcatgaa gacaggtgag atagaagttg ctggacatca
    14221 ttactatcta gaagaatcag gagctatgaa gcaaggctgg cttaaaaagg caaatgattg
    14281 gtatttctac aagacagatg gttcacgagc tataggttgg attaaagaca aggataaatg
    14341 gtacttcttg aaagaaaatg gtcaattact tgtgaatggt aagacaccag aaggctatac
    14401 tgtggattca agtggtgcct ggttagtgga tgttccgatc gagaaatctg ctacaattaa
    14461 aactacaagt cattcagaaa taaaagaatc caaagaagta gtgaaaaagg atcttgaaaa
    14521 taaagaaacg agtcaacatg aaagtgttac aaattcttca actagtcaag atttgacatc
    14581 ctcaacttca caaagctctg aaacgagtgt aaacaaatcg gaatcagaac agtagtagaa
    14641 aagaaggttt tagggccttc tttttcctat caactctttt ctatttcctg ttattcatgt
    14701 tataatggat aaatatgaat aatcggagtg agactatgaa atacaaacgg attgtcttta
    14761 aggtgggtac ttcttctctg acaaatgagg atggaagttt atcacgtagt aaggtaaagg
    14821 atattaccca gcagttggct atgctgcacg aggctggtca tgagttgatt ttggtgtctt
    14881 caggtgccat tgcggctggt tttggagcct taggatttaa aaagcgtccg actaaaattg
    14941 ctgataaaca ggcttcagca gcggtagggc agggactttt gttggaagaa tatacgacca
    15001 atcttctctt gcgtcaaatc gtttctgcac aaatcttgct gacccaagat gactttgtgg
    15061 ataagcgtcg ttataaaaat gcccatcagg ctttgtcggt tttgctcaac cgtggggcaa
    15121 ttcctatcat caatgagaat gatagtgtcg ttattgatga gctcaaggtt ggggacaatg
    15181 acactctaag tgctcaagta gcggcgatgg tccaagcaga ccttttagtt ttcttgacag
    15241 atgtggacgg tctctatact ggaaatccta attcagatcc aagagccaaa cgcttggaga
    15301 gaatcgagac catcaatcgt gagattattg atatggctgg tggagctggt tcgtcaaacg
    15361 gaactggggg tatgttaacc aaaatcaagg ctgcaactat cgcgacggaa tcaggagttc
    15421 ctgtttatat ctgctcatcc ttgaaatcag attccatgat tgaggcggca gaggagaccg
    15481 aggatggttc ttactttgtt gctcaagaga aggggcttcg tacccagaaa caatggcttg
    15541 ccttctatgc tcagagtcaa ggttctattt gggttgataa aggggctgcg gaagctctct
    15601 ctcaacatgg aaagagtctt ctcttatctg gtatcgttga agcagaagga gtcttttctt
    15661 acggtgatat cgtgacagta tttgataagg aaagtggaaa atcacttgga aaaggacgcg
    15721 tgcaatttgg agcatctgct ttggaggata tgttgcgttc tcaaaaagcc aagggtgtct
    15781 tgatttaccg tgacgactgg atttccatta ctcctgaaat ccaactactt tttacagaat
    15841 tttagaggta aactatggtg agtagacaag aacaatttga acaggtacag gctgttaaaa
    15901 aatcgattaa cacagctagt gaagaagtga aaaaccaagc cttgctagcc atggctgatc
    15961 acttagtggc tgctactgag gaaattttag cggctaatgc cctcgatatg gcagcggcta
    16021 aggggaaaat ctcagatgtg atgttggatc gtctttattt ggatgcagat cgtatagaag
    16081 cgatggcaag aggaattcgt gaagtggttg ccttaccaga tccaatcggt gaagttttag
    16141 aaacaagtca gcttgaaaat ggtttggtta tcacaaaaaa acgtgtagct atgggtgtca
    16201 tcggtattat ctatgaaagc cgtccaaatg tgacgtctga tgcggctgct ttgactctta
    16261 agagtggaaa tgcggttgtt cttcgtagtg gtaaggatgc ctatcaaaca acccatgcca
    16321 ttgtcacagc cttgaagaag ggcttggaga cgactactat tcatccaaat gtgattcaac
    16381 tggtggagga tactagccgt gaaagtagtt atgctatgat gaaggccaag ggctatctag
    16441 accttctcat tcctcgtgga ggagctggct tgatcaatgc agtggttgag aatgcgattg
    16501 tacctgttat cgagacaggg actgggattg tccatgtcta tgtggataag gatgcagacg
    16561 aagacaaggc gctgtctatc atcaacaatg ctaaaaccag tcgtccttct gtttgtaatg
    16621 ccatggaggt tctgctggtt catgaaaaca aggcagcaag catccttcct cgcttggatc
    16681 aaatgctggt tgcagatcga aaagaagctg ggttggaacc aattcaattc cgcctagata
    16741 gcaaagcaag ccagtttgtt tcaggtcaag ctgctcaagc acaagacttt gataccgagt
    16801 ttttagacta tattctagct gttaaggttg tgagcagttt agaagaagcg gttgcgcata
    16861 ttgaatccca cagtacccat cattcggatg ctattgtgac ggaaaatgct gaagctgcag
    16921 catactttac agatcaagtg gactctgcag cggtgtatgt taatgcctca actcgtttca
    16981 cagatggagg acaatttggt cttggttgtg aaatggggat ttctactcag aaattgcacg
    17041 cgcgtggtcc aatgggcttg aaagagttga ccagctacaa gtatgtggtt gctggtgatg
    17101 ggcagataag ggagtaagag atgaagattg gatttatcgg tttggggaat atgggtgcta
    17161 gcttggcaaa atctgtcttg cagactagga cgtcagatga gattctcctt gccaatcgta
    17221 gtcaagctaa ggtagatgct ttcattgcag actttggtgg tcaggcttcc agcaatgaag
    17281 aaatgtttgc agaagcagat gtgatttttc taggagttaa gcctgctcag ttttctgaac
    17341 tgctttctca atatcagacc atccttgaaa aaagagaaag tcttcttttg atttcgatgg
    17401 cagctggatt gaccttagaa aaactagcaa gtcttatccc aagccaacac cgaattattc
    17461 gtatgatgcc taatacccct gcttctatcg ggcaaggagt gattagttat gccttgtctc
    17521 ctaattgcag ggctgaggac agtgagatct tttgtcagct tttagccaag gctggtctct
    17581 tggttgaatt aggagaaagc ttaatcaatg cagcgacagg tcttgcaggt tgtggaccag
    17641 cctttgtcta tctctttatt gaggccttgg cagatgcagg tgttcagacg ggattaccac
    17701 gagaaacagc cttgaaaatg gcagcccaaa ctgtggtagg agctgggcaa ttggtcctag
    17761 aaagccagca acatcctgga gttttgaaag atcaagtttg tagtccaggc ggttcgacta
    17821 ttgctggcgt agcaagctta gaagcgcatg cttttcgagg catagtcatg gaggcagttc
    17881 atcaagccta taaacgaaca caagaactag gtaaataaga ggtagctttg actgcctctt
    17941 ttatggtggc tgaaatgaga agacacaaaa agattgtcac aaactcctat ttttttgata
    18001 gaatagaagt agtaaaaaag aaatgagtta gacatgtcaa aaggattttt agtctctctt
    18061 gagggaccag agggagcagg caagaccagt gttttagagg ctctgctacc aattttagag
    18121 gaaaaaggag tagaggtgct gacgacccgt gaacctggcg gagtcttgat tggggagaag
    18181 attcgggaag tgattttgga tccaagtcat actcagatgg atgctaaaac agagctactt
    18241 ctctatattg ccagtcgcag acagcatttg gtggaaaaag ttcttccagc ccttgaagct
    18301 ggcaagttgg tcatcatgga tcgttttatc gatagttctg ttgcctatca gggatttggt
    18361 cgtggcttag atattgaagc cattgactgg ctcaatcagt ttgcgacaga tggcctcaaa
    18421 cccgatttga cactctattt tgacatcgag gtggaagaag ggctggctcg tattgctgct
    18481 aatagtgacc gcgaggttaa tcgtttggat ttggaagggt tggacttgca taaaaaagtt
    18541 cgtcaaggct acctttctct tctggataaa gagggaaatc gcattgtcaa gattgatgct
    18601 agtctccctt tggagcaagt tgtggaaact accaatgctg tcttgtttga cggaatgggc
    18661 ttggccaaat gaaacaagat caactaaagg cttggcaacc agctcagttt gaccgttttg
    18721 tccgtatctt agaacaagac cagctcaatc acgcctatct cttttcaggt ttctttggaa
    18781 gcttggaaat ggcgcaattt ttagctaaga gccttttttg tacggataaa gttggcgtct
    18841 taccatgtga gaaatgccga agttgcaagc tgattgaaca ggaagagttt ccagatgtca
    18901 ccttgattaa gccagtcaat caggtcatca agacagaacg tattcgggaa ttggtgggac
    18961 agttttctca agcagggatt gaaagccagc aacaggtctt tatcatcgag caagcggata
    19021 aaatgcatcc caacgcagcc aattctctgc tcaaggtcat cgaagaaccc cagagtgaag
    19081 tttatatttt cttcttgact agcgatgagg aaaagatgtt accgacaatc cgaagtcgga
    19141 ctcagatctt ccactttaaa aagcaagaag aaaaacttat cttactctta gaacaaatgg
    19201 gacttgttaa gaaaaaagcg actcttttag ctaagtttag tcaatcgcga gctgaagcag
    19261 aaaagttggc acatcaggca agtttttgga ccttggtcga tgaaagtgaa cgcctgctga
    19321 cttggttagt agctaagaaa aaagaaagtt atctacaggt tgccaaatta gccaacttgg
    19381 cagatgataa ggaaaaacag gatcaggttt tacggattct tgaagttctc tgtgggcagg
    19441 acctcttgca ggtaagagta agagtgattc tacaagattt actagaagct agaaaaatgt
    19501 ggcaagctaa tgtcagcttt caaaatgcca tggaatatct ggtcttgaaa gaaatataaa
    19561 ctcaaaaatg aatgataaag aaaggaaagg gctgttttat ggacaaaaaa gaattatttg
    19621 acgcgctgga tgatttttcc caacagttat tggtaacctt ggccgatgtg gaagccatca
    19681 agaaaaatct caagagcctg gtagaggaaa atacagctct tcgtttggaa aattctaagt
    19741 tgcgagaacg cttgggtgag gtggaagcag atgctcctgt caaggccaag catgttcgtg
    19801 aaagtgtccg tcgcatttac cgtgatggat ttcacgtatg taatgatttt tatggacaac
    19861 gtcgagagca ggacgaggaa tgtatgtttt gtgacgagtt gctatacagg gagtaggcat
    19921 gcagattcaa aaaagtttta aggggcagtc tccctatggc aagctgtatc tagtggcaac
    19981 gccgattggc aatctagatg atatgacctt tcgagctatc cagaccttga aagaagtaga
    20041 ttggattgct gctgaggata cgcgcaatac aggtcttttg ctcaagcatt ttgacatttc
    20101 caccaagcag atcagttttc atgagcacaa tgccaaggaa aaaattcctg atttgattgg
    20161 tttcttgaaa gcagggcaaa gtattgctca ggtctctgat gccggtttgc ctagcatttc
    20221 agaccctggt catgatttag ttaaggcagc tattgaggaa gaaattgcag ttgtgacagt
    20281 tccaggtgcc tctgcaggaa tttctgcctt gattgccagt ggtttagcgc cacagccaca
    20341 tatcttttac ggttttttac cgagaaaatc aggtcagcag aagcaatttt ttggcttgaa
    20401 aaaagattat cctgaaacac agatttttta tgaatcacct catcgtgtag cagacacgtt
    20461 ggaaaatatg ttagaagtct acggtgaccg ctccgttgtc ttggtcaggg aattgaccaa
    20521 aatctatgaa gaataccaac gaggtactat ctctgagtta ttagaaagca ttgctgaaac
    20581 gccactcaag ggcgaatgtc ttctcattgt tgagggtgcc agtcagggtg tggaggaaaa
    20641 ggacgaggaa gacttgttcg tagaaattca aacccgcatc cagcaaggta tgaagaaaaa
    20701 tcaagctatt aaggaagtag ctaagattta ccagtggaat aagagtcaac tctacgctgc
    20761 ctaccacgac tgggaagaaa aacaataaag ggagatagga tgtaataatt ctgtctgttt
    20821 ctgtttaact taattagtga tgataatata aagatgtatc acttggtata gaagctttgg
    20881 tattaagttt tttattaagc ccatacggaa taccgatggt tggagcagca gttatagcgt
    20941 tcttagaagg tataaataga aaaataaggt cattttaaat caaaggattg ataaatcaga
    21001 aagaaggtga ttttttgcga acatacgaaa ataaagaaga actaaaagct gagatagaga
    21061 aaacatttga gaaatatatt ttagaatttg ataatattcc agaaaattta aaagataaga
    21121 gagctgatga agttgacaga actccagcag aaaaccttgc ttatcaggtt ggttggacca
    21181 acttggttct taaatgggaa gaagatgaaa gaaaggggct tcaagtaaaa acaccatcgg
    21241 ataaatttaa atggaatcaa cttggtgaat tatatcagtg gttcacagat acctacgctc
    21301 atttatctct gcaagagttg aaagcaaaat taaatgaaaa tattaattct atctctgcaa
    21361 tgattgattc gttgagtgag gaagaattat ttgaaccgca tatgagaaag tgggctgatg
    21421 aagcgactaa aacagcgact tgggaagtgt ataagtttat tcatgtaaat acggttgcac
    21481 cttttggaac tttcagaact aaaatcagaa aatggaagaa gatagtatta taaattatat
    21541 ttttaacttt aaaaaatttc ataaaaatgg ttaccaaagg cgatagaaga aaaactatcg
    21601 tctttttctt tgcaaatttt taagaaggga ggtgatcttg catggacttt gaatattttt
    21661 ataacagaga agcggaaaga tttaacttct taaaagtacc ggagatatta gttgatagag
    21721 aagaatttcg gggcttatca gcagaagcaa ttatccttta ttccatactt cttaaacgga
    21781 caggaatgtc atttaagaat aactggatag acaaggaagg cagagtattt atctatttta
    21841 ctgtcgaaga aattatgaaa agaagaaata tctcaaagcc aactgccata aaaacattag
    21901 atgagcttga tgtaaaaaaa aggaatagga ctgatcgaaa gagtaaggct tggacttggt
    21961 aagccgaaca tcatttatgt taaagacttt atgagtatat ttcaggtaaa agaaaatgac
    22021 ttacaaaagt caaaaaactt aacttcagaa gtaaaagatt ttaacctcag aagtaaagaa
    22081 aatgaacttc aagaggttaa gaaccttgac tctaactata tagagaataa taagagtaag
    22141 tatagtaaga gagaatatag ttttggtgaa aacggacttg gaacatttca aaatgtgttt
    22201 ttagctgttg aagatatatc ggatttacaa atcataatga actcacagct tgagaattac
    22261 attagacttc ctgcaaaact agaatcctag ttcatgattg ataataccag caatcaaatt
    22321 cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt gttgaaaaca ttttaaacgt
    22381 ttttactttg gcaaagatgt tctcaacctt gcttctttcc ttagatagcg catggttata
    22441 ggctttatct tcagctatta gcgacttgag tttgctggat ttacgtggag tttgtacttg
    22501 aggatatatc ttcatgagcc cttgataacc actgtcagcc aagattttac cagcttgtcc
    22561 gatatttctg caactcattt tgaacaactt catatcacga caatagttca cagtgatatc
    22621 caaagaaaca attctccctt gacttgtgac aatcgcttga gccttcatag cgtggcattt
    22681 ctttttacca gaatcattcg ctaattcttt ttttagggcg attgatttgt acttccgtcg
    22741 catcaatcat taccgtgtcc tcagagctga gaggagttct tgaaaccgta acaccacttt
    22801 gaacaagagt tacttcaacc cattggctcc gacggataaa gttgctttcg tgaataccaa
    22861 aatcagccgc aatttgttca taagttcgat attctcgcac tattgaagag tggccataag
    22921 aaggtcttct aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc
    22981 tgtttttaat acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt
    23041 aaatcgtgca tcagttagtt gtttacttgc ttcatcattc atagaactac tataccatat
    23101 tttgtttcgc aggaagtcta ttggaaagta agaaatattg aagctgaggc tattagaaga
    23161 aattgtgagc gtggtgctat tttttcaggt aaaataaaat atcacgaaga ttcacagttt
    23221 aaaggagatc actatgttga atgttatgct gttttagata atacggttat agcaagagat
    23281 agaataacag tccctatcga tccgttatgt ggaaaagatt ttatagagta gcatataatt
    23341 gattcttaac tggaatactc tttatctctt tacatcaaga aaatgactaa acagggaagt
    23401 ttgccttctt cccttttttt gttatactag tagaagaaaa aaatagaaag atttgtggga
    23461 gtgaaaaagt cctgtggact ttttcagcct gagccaagaa actcgaaagc tcgtaagtct
    23521 gattggcttt ttcaatgtga atcttaactt catactccca aagaggtatt agtgtcgtgt
    23581 ctcaatctta tatcaatgtt atcggtgctg gtttggcagg ttctgaagca gcctaccaaa
    23641 tcgcagagcg tggtattcca gttaaacttt atgaaatgcg tggtgtcaag tcaacacctc
    23701 agcacaaaac agacaatttt gctgagttgg tttgttccaa ttccttgcgt ggagatgctt
    23761 tgacaaatgc tgttggcctc cttaaggaag aaatgcgtcg cttgggttct gtgattttgg
    23821 aatctgctga ggctacacgt gttcctgcag gtggtgccct tgcagtggac cgtgatggtt
    23881 tctctcaaat ggtgaccgaa aaagttgcca accacccctt gattgaagtg gttcgtgatg
    23941 aaattacaga attgccgaca gatgttatta cggttatcgc tactggtcct ttgacaagtg
    24001 atgccttggc tgaaaagatt catgccctta atgacggtga tggtttttat ttctacgatg
    24061 cggcagcgcc tattatcgat gtcaacacta tcgatatgag caaggtctac ctcaaatcac
    24121 gttatgataa gggagaagcg gcctacctca atgcccctat gaccaagcaa gaatttatgg
    24181 atttccatga agctttggtc aatgcagaag aagcaccgct taattctttt gaaaaagaaa
    24241 agtactttga aggatgtatg cctatcgaag tcatggccaa acgtggcatt aaaaccatgc
    24301 tttatggtcc tatgaaacca gttggtcttg agtatccgga cgactacaca ggacctcgtg
    24361 atggagaatt taaaacacct tatgcggttg tccagctccg tcaggataat gcagctggta
    24421 gcctatacaa tattgttggt ttccagaccc acctcaaatg gggagaacaa aagcgtgtct
    24481 tccaaatgat tccgggtctt gaaaatgcgg agtttgtccg ttatggtgtc atgcatcgca
    24541 attcttacat ggattcacca aatcttcttg agcagactta ccgttccaag aaacaaccaa
    24601 atctcttctt tgctggtcag atgacggggg tggaaggcta tgttgagtcg gcggcttcgg
    24661 gcttagttgc gggaattaac gcagctcgtc tcttcaagga agaaagcgag gttattttcc
    24721 ccgagacgac agcgattgga agcttagctc attacattac ccatgccgac agcaaacatt
    24781 tccaaccaat gaatgtcaat tttgggatca tcaaggagtt ggaaggcgag cgtatccgtg
    24841 ataagaaggc tcgttatgaa aaaattgcag agcgtgccct tgccgactta gaggaatttt
    24901 tgactgtcta atttttttga aagaattgct catgatacta taaaaatctt agaaattgtg
    24961 ataaaatagg taggatgaaa gaaggagagt gaaaatggcg aatcccaagt ataaacgtat
    25021 tttaatcaag ttatcaggtg aagcccttgc cggtgaacgt ggcgtaggga ttgatatcca
    25081 aacagttcaa acaatcgcaa aagagattca agaagttcat agcttaggta tcgaaattgc
    25141 ccttgttatt ggtggaggaa atctttggcg tggagaacct gcagcagaag caggtatgga
    25201 ccgtgttcag gcagattaca ctggaatgct tgggactgtt atgaatgctc ttgtgatggc
    25261 agattcattg caacaagttg gggttgatac gcgtgtacaa acagctattg ctatgcaaca
    25321 agtggcagag ccttatgtcc gtggacgtgc ccttcgtcac cttgaaaaag gccgtatcgt
    25381 tatctttggt gctggaattg gttcaccata cttctcgaca gatacaacag cggcccttcg
    25441 tgcagctgaa atcgaagcag atgccatcct catggctaaa aatggcgtcg atggtgtgta
    25501 caatgccgat cctaagaagg acaagacagc cgttaagttt gaagaattga cccaccgtga
    25561 tgttatcaac aaaggtcttc gtatcatgga ctcaacagcc tcaaccctct caatggacaa
    25621 cgacattgac ttggttgtct tcaacatgaa ccaatcaggc aacatcaaac gtgtcgtatt
    25681 tggtgaaaat atcggaacaa cagtttcaaa taatatcgaa gaaaaggaat aagaaagaat
    25741 atggctaacg taattattga aaaagctaaa gagagaatga cccaatctca ccaatcactt
    25801 gctcgtgaat ttggtggtat ccgtgctggt cgtgccaatg caagcttgct tgaccgtgta
    25861 catgtagaat actatggagt cgaaactcct cttaaccaaa tcgcttcaat tacgattcca
    25921 gaagcgcgtg ttttgttggt aacaccattt gacaagtctt cattgaaaga catcgaacgt
    25981 gccttgaacg cttctgatct tggtatcaca ccggctaatg acggttctgt gattcgcttg
    26041 gttatcccag ctcttacaga agaaactcgt cgtgaccttg ctaaagaagt gaagaaggtc
    26101 ggcgaaaatg ctaaagtggc tgtccgcaat atccgtcgcg atgctatgga cgaagctaag
    26161 aaacaagaaa aagcaaaaga aatcactgaa gacgaattga agactcttga aaaagatatt
    26221 caaaaagtaa cagacgatgc tgttaaacac atcgacgaca tgactgctaa caaagagaaa
    26281 gaacttttgg aagtctaaaa ataaacagaa aaactcagtt ggcattgctg gctgagtttt
    26341 attcgaaaga aggaaatatg aatacaaatc ttgcaagttt tatcgttgga ctgatcatcg
    26401 atgaaaatga ccgtttttac tttgtgcaaa aggatggtca aacctatgct cttgctaagg
    26461 aagaaggtca acatacagta ggggatacgg tcaaaggttt tgcatacacg gatatgaagc
    26521 aaaaactccg cctgacaacc ttagaagtga ctgccactca ggaccaattt ggttggggac
    26581 gtgtcacaga ggttcgtaag gatttgggtg tctttgtgga tacaggcctt cctgacaagg
    26641 aaatcgttgt gtcactcgat attctccctg agctcaagga actctggcct aagaagggcg
    26701 accaactcta catccgtctt gaagtggata agaaagaccg tatctggggc ctcttggctt
    26761 atcaagaaga cttccaacgt cttgctcgtc ctgcctacaa caacatgcag aaccaaaact
    26821 ggccagccat tgtttaccgt ctcaagctgt caggaacttt tgtttaccta ccagaaaata
    26881 atatgcttgg ttttattcat cctagcgagc gttacgcaga gccacgtttg gggcaagtat
    26941 tagatgcgcg cgttattggt ttccgtgaag tggaccgcac tctgaacctc tccctcaaac
    27001 cacgctcctt tgaaatgttg gaaaacgatg ctcagatgat tttgacttat ttggaaagta
    27061 atggcggttt catgacctta aatgataagt catctccaga cgacatcaag gcaacctttg
    27121 gcatttctaa aggtcagttc aagaaagctt taggtggtct tatgaaggct ggtaaaatca
    27181 agcaggacca gtttgggaca gagttgattt agggaggctt atgagaaaat cattttacac
    27241 ttggctcatg accgagcgca atcctaaaag taacagtccc aaagcaattt tggcagacct
    27301 cgcttttgaa gaggcagctt ttccaaaaca tacagatgat tttgatgagg tcagtcgctt
    27361 tttggaggag catgccagtt tctcttttaa cctaggagat tttgacagca tttggcagga
    27421 atatctagaa cactagcatt tattcattgg gtttgggcta gtaatttctc catccctctg
    27481 ctataataaa aagaaataaa aggattagag aggttcttta tttgaaggaa cattcaatag
    27541 acattcaact gagtcatcca gatgacctgt ttcatctttt tggttccaat gaacgccatc
    27601 ttcgtttgat ggaagaagag cttgatgttg tgattcatgc tcgtacggag attgtccagg
    27661 ttttgggaga agagtctgcc tgtgaggaag cccgtcaagt tattcaggct ttgatggtct
    27721 tggtaaatcg tgggatgacc gttggtacgc cagatgtagt cactgcgatt agcatggtca
    27781 aaaatgatga aattgacaag tttgtcgccc tttacgaaga agaaattatc aaagataata
    27841 ctgggaaacc tatccgtgtc aaaaccctag ggcaaaagct ttatgtggac agtgtcaaac
    27901 agcatgatgt gacctttgga attgggccag caggtacagg gaagaccttc cttgcagtga
    27961 ccttggcagt gactgccctt aaacgtgggc aagtcaagcg aattatccta actcgtccag
    28021 cggtggaagc gggagagagt cttggatttc ttccgggtga tcttaaggag aaggtggatc
    28081 cttaccttcg tcctgtttac gatgccttgt atcagattct ggggaaagac caaacaactc
    28141 gactcatgga gcgtgaaatt atcgagattg cgccccttgc ctatatgcgt ggacggacct
    28201 tggatgatgc ctttgtcatt ctcgatgagg cgcaaaatac gaccatcatg cagatgaaga
    28261 tgttcttgac tcgtttaggt tttcattcta agatgattgt caatggagat attagtcaga
    28321 ttgacctgcc acgtaatgtc aagtccggtt tgattgatgc tcaagagaaa ctcaagaaca
    28381 tccatcagat tgactttgtt catttttcag ccaaggatgt ggttcgccat ccagttgtcg
    28441 ctcagattat ccgagcctat gaatattcta ctgaagttgc acacgactga ttttgaggaa
    28501 gtttgcctgc aaaagaatat acttttgaga gaggaaaatc cagttgtata ggctaaaggt
    28561 tttatccaaa ggtttgagac aacgattagg cacgatggaa agaactttta tgtggctgat
    28621 gacgatcagt gcatcttcct gtgtcataat cacagggcac aagaaagtag gaatttgaaa
    28681 agatgattga ccaactatct aagtattaca gttgtaggat actaactgaa aaggatattc
    28741 caagtatttt atctttatat gaaagtaatc ctctgtattt tcagcattgt ccaccagagc
    28801 caaattttgc aactgtaaaa gaggacatgc tttgtctacc tgaaggtaaa gctaaggctg
    28861 ataagttttt tgttggattt tggaatggat ctgaccttgt ggctgttata gattttgtct
    28921 atgcacatcc tgatgaggag actgttttta ttggtttgtt tatggttgat caagcctatc
    28981 agaggaaagg gattggtagt catattgtga cagaagcact agcttatttt gctaagaact
    29041 ttcgaaaggc acgtttggct tatgttaagg gaaatccgca atctcagcat ttttgggaaa
    29101 agcagggctt taaatcaatt ggatgcgagg ttaagcaaga actctatacg gttgttatcg
    29161 ctgaacagag cctagaagat tagaaatggc atcaagtaag aactatttgg aatttgtttt
    29221 ggaacaatta tcaggattag atgatgtgac ttaccgttcc atgatggggg agtatattct
    29281 ttacttccgc ggcaagatta ttggcggcat ttatgacgat cgctttttag ttaaacccgt
    29341 gcaagcagtc ttagataaga ttgaccaatc ttcttttgag tttccataca aaggtgccaa
    29401 agaaatgatt tgagtggaag aacttgataa taagatgttt ctataagacc tgattttagc
    29461 tatgtataac caactgccaa cgcccaaacc taaaaagaaa aagcaagggt gaacgaagta
    29521 aaaaagaagt ctgctaaggc cctgtctttg cacgggtaaa attttatata taaaaagaag
    29581 ctgggactaa agagctcagc ttcctttggt ttatataatt gtcattacaa gacgaagtgg
    29641 ttgggcgaaa ctctgttgac tttattcaat ttagagtttc ttatgcacaa ttgagtctgg
    29701 aacgaaagtc tccagttgca aagtatacag tacaataaac caacgatgta atagctgatg
    29761 acacaaagca cagtgggtag gacttgcgaa gtcacccttt tcttttcaaa atttatacta
    29821 aatcattgat atcagtgtag tcacgattaa gtccttgagc aactggtagg ctagtcaagt
    29881 aaccttgata agtggtcaca ccttgacgca agccttcatc ttcagagatt gcttgtgcga
    29941 atcctttgcc agccaaagct tcgatataag gaagagtgac attggttagg gcgatggttg
    30001 aagtgcgggc aaccgcacca gggatattgg caacggcata gtggagaaca ccgtgttttt
    30061 catagacggg ttcatcgtgc gttgtcacac ggtcagctgt ttcgataacg ccaccttggt
    30121 caacagcaac gtcaacgata cagagcctgg acgcatttgt ttgaccatct catctgtcac
    30181 caattccggt gcttttgcac cagggatgag aatggctcca atcaccacat cagcatctct
    30241 catacttgct tcaatgttga atgaattaga tataagagtt tgaatttgac ttccaaagac
    30301 ttcttctaga actgagagac gcttggcact aatatctaaa atagtcactt gagcaccaag
    30361 accaagggcg atgcgggcag catgtgtacc gacgacacca tcaccgatga tagttacttt
    30421 tccttttgga acacctggta caccaccagg tagaacacca gagccaccag cttgcttagt
    30481 aaggaagtga gctccgattt aaacagccat acgacctgca acctcactca taggaacgag
    30541 gagcggtagt tgtccttgat tgtcacgaac agtttcatag gcaattcctg ttgtttttgc
    30601 tgctaacata gcatctgcta attctggagc agcggccatg tgcaagtagg tgaagagaag
    30661 aagatcgtcg cgcaagtaac cgtattcaga acttaaaggt tcttttactt tcacaaccaa
    30721 ctctgctgcc caagcttcac cagcagtagc gacaatctca gctccttgct tttgatagtc
    30781 agcatcagta aagccagaac cgagaccagc atttgtttcg ataaggacac gatgaccacg
    30841 actaactaag ctatgaacac ctgcaggtgt gagggcgaca cggttttcgt tatttttaat
    30901 ttcttttggg attccgatta acattgagat aacctacctt tcaattgacg gtcttgtttt
    30961 ggttgtcaca ttccagttca taaatcaaaa atgtgacggt ttcattgtat atgaaaccgc
    31021 ttcaaaaatc aagaaaaact tgtcatccaa atttttttat gctagactag tgaaaatcaa
    31081 gctctaatgg agggaaaagt atggaatcaa tatttgtgaa atttgcccag tatccgtcta
    31141 tagaaacgga gcgtttattg cttagacctg taactttgga tgatgcggaa gcaatgtttg
    31201 actatgcctc ggacaagggt aatacacgtt acacttttcc aaccaatcaa agcttggaag
    31261 aaaccaagaa taacattgct cagttctact tggctaatcc cttgggacgt tgggggattg
    31321 aattaaaaga taacggcaag tttatcggaa ctattgactt acacaagatt gatcctgttc
    31381 ttaagaaggc agctattggc tacattatca ataaaaagta ttggaatcaa ggattaacgc
    31441 cagaaaccaa tcgtgctgtg attgagctag cttttgagaa gataggaatg aataagttag
    31501 ttgctttata cgataaggac aattctgcgt caggaaaggt catggagaaa tcaggcatgc
    31561 gtttttccca tgcagaacca tatgcttgta tggaccagca tgaaaaaggc cgaatcgtga
    31621 caagagttca ttatgtcttg accaaggaag actattttgc aaataaataa gcagttgaaa
    31681 agaaattttt cgactgtttt ttcttcctct tacgaataat ctaagagagg agaaaatatg
    31741 gaagcaatta tcgagaaaat caaagagtat aaaatcatcg tcatctgtac tggtctgggc
    31801 ttgcttgtag gcggattttt cctgctaaag ccagctccac aaacacctgt aaaggaaacg
    31861 aatttgcagg ctgaagttgc agctgtttcc aaggatttgg tatccgaaaa ggaagtgaac
    31921 aaggaagaga aggaagaacc ccttgaacaa gatctaatca cagtagatgt caaaggtgct
    31981 gtcaaatcgc cagggattta tgacttgcct gtaggtagtc gagtcaatga tgctgttcag
    32041 aaggctggtg gcttgacaga gcaagcagac agcaagtcgc tcaatctagc tcagaaagtt
    32101 agtgatgagg ctctggttta cgttcctact aagggagaag aagcagttag tcaacagact
    32161 ggttcgggga cagcttcttc aataagcaag gaaaagaagg tcaatctcaa caaggccagt
    32221 ctggaagaac tcaagcaggt caagggactg ggaggaaaac gagctcagga cattatcgac
    32281 catcgtgagg caaatggcaa gttcaagtca gtagacgagc tcaagaaggt ctctggcatt
    32341 ggtggcaaaa caatagaaaa gcttaaagac tatgttacag tggattaaga atttctctat
    32401 tcccctaatt tacctgagtt ttctattact ttggctttat tacgctattt tctcagcatc
    32461 ttatcttgct ttgttgggct ttgtttttct gctagtctgt ctctttatcc aatttccgtg
    32521 gaaatctgct ggtaaagttc taataatttg cggaatcttt ggattttggt ttgtttttca
    32581 aaattggcaa cagagtcaag cgagtcaaaa tctggcggat tctgttgaaa gggtacggat
    32641 tttgcctgat actattaagg ttaatggtga tagtctatcc tttcgtggca agtctaacgg
    32701 tcgtgctttc caagtctatt ataaactcca gtccgaggag gagaaagaag cctttcaagc
    32761 tttaaccgac ctgcatgaga taggactaga agggaagctt tcggagccag aagggcagag
    32821 aaattttggt ggctttaatt accaagccta tctgaagact cagggaattt accagactct
    32881 caatatcaaa acaatccagt cacttcaaaa gattggcagt tgggatatag gagaaaactt
    32941 gtccagttta cgtcgaaagg ctgtggtttg gattaagacg cactttccag accctatgcg
    33001 caattacatg acaggactct tgctgggaca tctggacacc gactttgagg agatgaatga
    33061 gctttattcc agtctaggaa ttatccacct ctttgcccta tctggtatgc aggtaggttt
    33121 tttcatgaat ggatttaaga aacttctctt gcgattgggc ttgacccaag aaaagttgaa
    33181 atggctgact tatccctttt cccttatcta tgcgggacta actggatttt cagcatcggt
    33241 tattcgcagt ctcttgcaaa agctactggc tcaacatggg gttaagggct tggataattt
    33301 tgccttgacg gtgcttgtcc tctttattgt catgccaaac tttttcttga cagcaggagg
    33361 agtcttgtcc tgcgcttatg cttttatcct gaccatgacc agcaaagaag gggaggggct
    33421 caaggctgtt actagtgaaa gtctagtcat ctccttgggc atattgccca ttctatcctt
    33481 ctattttgcg gaatttcaac ctttgtctat ccttttgacc tttgtctttt cctttctatt
    33541 tgacttgacc ttcttaccgc tcttgtctat tttatttgtc ctttcctttc tctatccagt
    33601 cattcagctg aactttatct ttgaatggtt ggagggcatt attcgcttgg tgtcacaggt
    33661 gacaagtaga cctctggtct ttggacaacc caatgcatgg tttttaatcc tattgttaat
    33721 ttccttggct ttggtctatg atttgagaaa aaacattaaa aagctaacgg tattgtgctt
    33781 attgattaca gggctctttc tcctgaccaa gcatccactg gaaaatgaaa tcaccatgct
    33841 ggatgtgggg caaggagaaa gtattttcct acgggatgta actgggaaaa ctattctcat
    33901 agatgtaggt ggtaaggcag aatcttataa gaaaatcgaa aaatggcaag aaaaaatgac
    33961 gaccagcaat gcccagcgaa ccttgattcc ctatctcaaa agtcgaggag tagctaagat
    34021 tgaccagcta attttgacta acacggacaa ggagcatgtt ggagatttgt cagaggtgac
    34081 caaggctttc catgtagggg agattctagt atcaaaagac agtctgaaac agaaggaatt
    34141 tgtggcagaa ctacaggcga ctcaaacaaa ggtgcgtagt atgacagtag gggagaactt
    34201 gcccattttt ggaagtcagt tagaagttct atctccaagg aaaatgggag atggaggaca
    34261 cgatgatacc ctagttctgt atgggaaatt cttggataag caatttctct tcacgggaaa
    34321 tttggaggag aaaggagaga aggacttgct gaagcactat ccagacttga aagtaaatgt
    34381 tttgaaagct agccaacatg gcaataaaaa atcatcaagt ccagcctttc tagaaaaact
    34441 caaaccagag cttactctta tctcagttgg aaagagcaat cgaatgaaac tcccccatca
    34501 ggaaacattg acacgactgg aaggtatcaa tagcaaagtt tatcgaactg accagcaagg
    34561 agctatacgt tttaaggggt tggatagttg gaaaatcgaa agtgttcgat aggaaggata
    34621 aatgttgtag attagtgaaa taaactaaaa atttgttgca taataatgat aaaaatggta
    34681 taatgaaaac gtattcaata ttgaggatat aaaatcatta aaaattagca aaaatcgttt
    34741 aattagttag tttatgatta ttggtctttt tcagtccagt atatctgctg tgacagtcac
    34801 taaaagttac aagtatgatt ggaatacggt ttgggaatat agtaccaact atcacgacca
    34861 tcagtatgct tggattccgt catggtctcg ttatgacagc tattctgagt atccagttgg
    34921 ttctggctgg aactacggtc gttatgagat cataaactat tacagcggag gctattaatt
    34981 cttaaagagt gagaaaaagg agggctagat atgttgcagc ttactcatgt gaccttaaaa
    35041 acgcgacaag tcatcttgca agatgtggat ttcaccttta aaaagggtag ggtttatggt
    35101 cttcttgcta tcaatggctc tggaaagacg accctgttcc gtgccattag caatttaatt
    35161 cccataagta gtggaaatat cgcagcccct ccttctttat tttattatga gagtattgaa
    35221 tggctggatg gaaacttaag tgggatggac taccttcgtc ttatcaaaaa catctggaag
    35281 tcaggtctga acttgaggga tgaaatcgcc tattgggaaa tgtctgacta tatcagtctt
    35341 cccattcgca agtattcctt aggcatgaag caacgcttgg tgattgccat gtatttcctc
    35401 agtcaggcca aatgctggct catggatgag attacaaatg gcttagatga gtattatcga
    35461 cagaagtttt ttgataggct agcacaaatc gatagacaag aacagctggt tcttttaagt
    35521 tcccactata aggaagagtt ggttgatgtc tgcgatagag tagtaaccat tcatcagggg
    35581 cagatagaag aggtttagtt tatgaaagat gttagtctat ttttattgaa aaaagttttc
    35641 aaaagccgct taaactggat tgtcttagct ttatttgtat ctgtactcgg tgttaccttt
    35701 tatttaaata gtcagactgc aaactcacac agcttggaga gcaggttgga aagtcgcatt
    35761 gcagccaacg agagggctat caatgaaaat gaagagaaac tctcccaaat gtctgatacc
    35821 agctcggagg aataccagtt tgctaaaaat aatttagacg tgcaaaaaaa tcttttgacg
    35881 cgaaagacag aaattctaac tttactaaaa gaagggcgct ggaaagaagc ctactatttg
    35941 cagtggcaag atgaagagaa gaattatgaa tttgtatcaa atgacccgac tgctagctct
    36001 ggcttaaaaa tgggggttga ccgcgaacgg aagatttacc aagccctgta tcccttgaac
    36061 ataaaagcac atactttaga gtttccgacc cacgggattg atcagattgt ctggatttta
    36121 gaggttatca tcccaagctt gtttgtggtt gcgattattt ttatgctaac acaactattt
    36181 gcagaaagat atcaaaatca tctggacaca gctcacttat atcctgtttc aaaagtgaca
    36241 tttgcaatat cctctcttgg agttggagtg ggatatgtaa ctgtgctgtt tatcggaatc
    36301 tgtggctttt cttttctagt gggaagtctg ataagtggtt ttggacagtt agattatccc
    36361 tacccgattt atagcttagt gaatcaagag gtaactattg gaaagatgca ggatgtatta
    36421 tttcctggct tgctcttagc tttcttagcc tttatcgtca ttgtggaagt tgtgtatttg
    36481 attgcttact ttttcaagca aaaaatgcct gtcctctttc tttcactcat tgggattgtt
    36541 ggcttattgt ttggcatcca aaccattcag cctcttcaaa agattgcaca tctgattccc
    36601 tttacttact tgcgttcagt ggagatttta tctggaagat tacctaagca gattgataat
    36661 gtcgatctaa attggagcat ggggatggtc ttacttcctt gcctgattat ccttttgtta
    36721 gtggggattc tatttatcga aagatgggga agttcacgta aaaaggaagt ttttaaagtt
    36781 tagttttcct atagggaagc taaagaaaaa ctaacatagg gagagaatca acttggttct
    36841 ctctttttga ttcgaaaacc aaaccaaaat acaaacacaa acttttcaaa aaataacttt
    36901 ttatcttgac aagagctaga aaacttggta tcatataaaa gttgagaaaa gcagaagtga
    36961 gagcttctcg ccttgtgaca ttaagttgcc tggccctacg gatgaaaagt ttcgaagaaa
    37021 cgctatcata acgtgcgggc ttgtatattt acgagtccgc tattgttttt ctctaataaa
    37081 acaaaagagg tgaaaaccat agcaaagcaa gacttattca tcaatgatga gattcgtgta
    37141 cgtgaagttc gcttgattgg tcttgaggga gaacagctag gcatcaagcc actcagtgaa
    37201 gcgcaagctt tggctgataa cgctaatgtt gacctagtat tgattcaacc ccaagccaaa
    37261 ccgcctgttg caaaaattat ggactacggt aagttcaaat ttgagtacca gaagaagcaa
    37321 aaagaacaac gtaaaaaaca aagcgttgtt actgtgaaag aagttcgtct aagtccaaca
    37381 attgacaagg gtgactttga tacaaaactt cgtaatgcac gtaagttcct tgaaaaagga
    37441 aataaagtta aggtatctat tcgctttaag ggtcgtatga ttacccataa agagattggt
    37501 gcaaaagttt tagccgagtt tgctgaagca actcaagata ttgccatcat cgaacaacgt
    37561 gctaaaatgg atggacgtca aatgttcatg caattggcgc cagcgactga caaaaaataa
    37621 ttgtcagaaa gttaaataaa ggagaaaaaa tcatgccaaa acaaaaaaca caccgcgcat
    37681 cagctaaacg tttcaaacgt acaggttctg gtggacttaa acgtttccgt gcttacactt
    37741 ctcaccgttt ccacggaaaa actaagaaac aacgtcgtca tcttcgtaaa gcatctatgg
    37801 tgcattcagg agattacaaa cgtatcaaag caatgcttac tcgcttgaaa taatagcttg
    37861 actgtaagta aacaattcta gaaaatattt ggaggaaata aaacatggca cgtgttaaag
    37921 gtggcgttgt atcacgcaaa cgtcgtaaac gtattcttaa attagcaaaa ggttactatg
    37981 gagctaaaca catcttgttc cgtactgcaa aagaacaagt aatgaactct tactactatg
    38041 cataccgtga ccgtcgtcag aaaaaacgtg acttccgcaa attgtggatt actcgtatca
    38101 atgcggcggc tcgtatgaac ggactttcat actcacaatt gatgcatggt ttgaaattgg
    38161 ctgagatcga agttaaccgt aaaatgcttg ctgacttggc tgttaacgat gcagtagctt
    38221 tcacagctct tgcagatgca gctaaagcaa aacttggtaa ataacaactg atgagactgg
    38281 aacaggattg tccagtcttt ttaaaaataa aggagaaaga tatggcttca aaaatgctac
    38341 acacttgctt acgagtagaa aatcttgaaa aatcaatcgc attttatcaa gatgcttttg
    38401 gttttaaaga attgcgtcgc agagattttc cagaccatgc cttcacgatt gtctatctag
    38461 gtcttgaggg tgatgattat gagttggagt tgacttataa ctacgatcac ggtccgtatg
    38521 tggttggtga tgggtttgcc catatcgccc tcagtacacc tgatcttgag gcacttcatc
    38581 aagaacacag tgcaaaaggc tatgaagtga ctgagccaaa tggtctacca ggaactacac
    38641 caaactatta ctttgttaaa gaccctgatg gatataaggt cgaagtcatt cgtgaaaaat
    38701 aatttcgtgc aagttaagta gaatattcgt tttctacttg acttttttta gctgaaagag
    38761 tatactaata aaaattcaac ctttaagggg agtccagaga gactcacaag gtgtcagata
    38821 aaagaatagt gcaattttct agaggagacc ttttgagtgt gctctcttgt gttgtacgat
    38881 tttaactgag gccttgcact agcaaggtct tttctttatc tggtcccctt aaaatttaag
    38941 gaggaaaagt tatgaatctc acatgtaaga agcgtttggg tgtcattcgg ttggaaacca
    39001 tgaaggtggt tgcacaagag gaaatcgcgc cagcaatctt tgaattagtc ctagaaggag
    39061 aaatggttga agccatgcga gcaggccaat ttcttcatct gcgtgtacca gacgatgccc
    39121 atctcttacg tcgtcctatt tcaatttcgt ctattgacaa ggcaaacaag cagtgtcacc
    39181 tcatttatcg gattgaggga gctgggactg caattttttc aaccttaagt cagggagaca
    39241 ctcttgatgt gatggggcct cagggaaatg gttttgactt gtctgacctt gatgagcaga
    39301 atcaggttct ccttgttggt ggtgggattg gtgttccacc cttgcttgag gtggccaagg
    39361 aattgcatga acgtggagtg aaagtagtga cagtcctcgg ttttgctaat aaggatgctg
    39421 ttattttgaa aacggaattg gctcagtatg gtcaggtctt tgtaacgaca gatgatggtt
    39481 cttatggcat caagggaaat gtttccgttg ttatcaatga tttagacagt cagtttgatg
    39541 ctgtttactc gtgtggggct ccaggaatga tgaagtatat caatcaaacc tttgatgatc
    39601 acccaagagc ctatttatct ctggaatctc gtatggcttg tgggatggga gcttgctatg
    39661 cctgtgttct aaaagtacca gaaagcgaga cggtcagcca acgcgtctgt gaagatggtc
    39721 ctgttttccg cacaggaaca gttgtattat aaggagaaaa ttatgactac aaatcgatta
    39781 caagtttctc tacctggttt ggatttgaaa aatccgatta ttccagcatc aggctgtttt
    39841 ggctttggac aagagtatgc caagtactat gatttagacc ttttaggttc tattatgatc
    39901 aaggcgacaa cccttgaacc acgttttgga aatccaactc caagagtggc agagacgcct
    39961 gctggtatgc tcaatgcaat tggcttgcaa aatcctggtt tagaggttgt tttggctgaa
    40021 aagctacctt ggctggaaag agaatatcca aatcttccta ttattgccaa tgtagctggt
    40081 ttttcaaaac aagagtatgc agctgtttct catgggattt ccaaggtaac taatgtaaaa
    40141 gctatcgagc tcaatatttc ttgtcccaat gttgaccact gtaatcatgg acttttgatt
    40201 ggtcaagatc cagatttggc ttatgatgtg gtgaaagcag ctgtggaagc ctcagaagtg
    40261 ccagtttatg tcaaattaac cccgagtgtg accgatatcg ttactgtcgc aaaagctgca
    40321 gaagatgcgg gagcaagtgg cttgaccatg atcaatactc tggttggaat gcgctttgac
    40381 ctcaaaacca gaaaaccaat cttggccaat ggaacaggtg gaatgtcagg tccagcagtc
    40441 tttccagtag ccctcaaact catccgccaa gtagcccaaa caacagacct gcctatcatt
    40501 ggaatggggg gagtggattc ggctgaagct gccctagaaa tgtatctggc tggggcatct
    40561 gctatcggag ttggaacagc taactttacc aatccttatg cctgccctga catcatcgaa
    40621 aatttaccaa aagtcatgga taaatacggt attagcagtc tggaagaact ccgtcaggaa
    40681 gtaaaagagt ctctgaggta aactgcaatc aatctgttct tgatttttta ttagtttgta
    40741 atatgaattt aggagaattt tggtacaata aaataaataa gaacagagga agaaggttaa
    40801 tgaagaaagt aagatttatt tttttagctc tgctattttt cttagctagt ccagagggtg
    40861 caatggctag tgatggtact tggcaaggaa aacagtatct gaaagaagat ggcagtcaag
    40921 cagcgaatga gtgggttttt gatactcatt atcaatcttg gttctatata aaagcagatg
    40981 ctaactatgc tgaaaatgaa tggctaaagc aaggtgacga ctatttttac ctcaaatctg
    41041 gtggctatat ggccaaatca gaatgggtag aagacaaggg agccttttat tatcttgacc
    41101 aagatggaaa gatgaaaaga aatgcttggg taggaacttc ctatgttggt gcaacaggtg
    41161 ccaaagtaat agaagactgg gtctatgatt ctcaatacga tgcttggttt tatatcaaag
    41221 cagatggaca gcacgcagag aaagaatggc tccaaattaa agggaaggac tattatttca
    41281 aatccggtgg ttatctactg acaagtcagt ggattaatca agcttatgtg aatgctagtg
    41341 gtgccaaagt acagcaaggt tggctttttg acaaacaata ccaatcttgg ttttacatca
    41401 aagaaaatgg aaactatgct gataaagaat ggattttcga gaatggtcac tattattatc
    41461 taaaatccgg tggctacatg gcagccaatg aatggatttg ggataaggaa tcttggtttt
    41521 acctcaagtt tgatgggaaa atagctgaaa aagaatgggt ctacgattct catagtcaag
    41581 cttggtacta cttcaaatcc ggtggttaca tgacagccaa tgaatggatt tgggataagg
    41641 aatcttggtt ttacctcaag tctgatggca aaatggctga aaaagaatgg gtctacgatt
    41701 ctcatagtca agcctggtac tacttcaaat ctggtggcta catggcgaaa aatgagacag
    41761 tagatggtta tcagcttgga agcgatggta aatggcttgg aggaaaaact acaaatgaaa
    41821 atgctgctta ctatcaagta gtgcctgtta cagccaatgt ttatgattca gatggtgaaa
    41881 agctttccta tatatcgcaa ggtagtgtcg tatggctaga taaggataga aaaagtgatg
    41941 acaagcgctt ggctattact atttctggtt tgtcaggcta tatgaaaaca gaagatttac
    42001 aagcgctaga tgctagtaag gactttatcc cttattatga gagtgatggc caccgttttt
    42061 atcactatgt ggctcagaat gctagtatcc cagtagcttc tcatctttct gatatggaag
    42121 taggcaagaa atattattcg gcagatggcc tgcattttga tggttttaag cttgagaatc
    42181 ccttcctttt caaagattta acagaggcta caaactacag tgctgaagaa ttggataagg
    42241 tatttagttt gctaaacatt aacaatagcc ttttggagaa caagggcgct acttttaagg
    42301 aagccgaaga acattaccat atcaatgccc tttatctcct tgcccatagt gccctagaaa
    42361 gtaactgggg aagaagtaaa attgccaaag ataagaataa tttctttggc attacagcct
    42421 atgatacgac cccttacctt tctgctaaga catttgatga tgtggataag ggaattttag
    42481 gtgcaaccaa gtggattaag gaaaattata tcgatagggg aagaactttc cttggaaaca
    42541 aggcttctgg tatgaatgtg gaatatgctt cagaccctta ttggggcgaa aaaattgcta
    42601 gtgtgatgat gaaaatcaat gagaagctag gtggcaaaga ttagtactat aagtgaatat
    42661 gatttgagtg aatagtaagt taaaaatcct gatttcaagt aaaatcagga ttttttcatg
    42721 gatgcaattt ttttggagtc tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca
    42781 aagccgggtt ttccaccagt tggtttattg agttttttga cttcaatcat atctacctgc
    42841 accagattcg acaggcgccc ttgagagaag taggcagcta actctgctgc gtctgtcttg
    42901 actgcatcag atgggtcaag atttcctgag atgacaacat ggcttccagg aatgtcctta
    42961 gcatggaacc aaagttcctc cttgcgggcc attttaaagg tcaattcctc attttgaaga
    43021 ttgtttcgtc cgacatagat gatggttttg ccatcgcttg ctagatattg ttctagtttt
    43081 ttgcgtttct ggattttctc ccgttgtctt ctgcggataa aacctgtttg aatcaattct
    43141 tcacggattt cagcgatttc ttccagtcca gcttggttga ggacggtttc tacactttcc
    43201 agatagagaa tagtggcttt ggtttcttca atcaaatcag tcaagtattt gacagcttct
    43261 ttgagtttct gataccgttt aaaatagcgt tgggcattct ggttgggagt cagagcctta
    43321 tcaagcccaa tcatgatagg ttggttggta tagtagttgt ctaggataac ctggtcttgg
    43381 tcgttaggca cttggtggag gaaggttgtc agcaattctc ctttttgacg aaattcttca
    43441 gcgttgtctg tcgccagtaa ttctttttcc tgttttttga gtttgtgtcg gtttttctga
    43501 agttcatttt caacacgacg aatcagttca ctggcctgct gtttgacgcg gtcgcgctca
    43561 gccttatcct tatagtaggt gtccaacaaa tcagaaagat ttgcaaaagg ctctcccacc
    43621 tgatttgcaa aaggaactgg actgaaggaa gtctcagtca agcatggctt ggtttcctga
    43681 ttgaaaaaat ttcggaaagc ggaaagtttt tcactaacca gtatcctttc caattcattt
    43741 gccgtatcgc gtcccagacc ttgaaagagg ctttgaagat tttttgctgt tagttcttgg
    43801 gtttgcagga tttcaaagag cttttcatcc ttgatagtaa aaggattgag agattctgta
    43861 cttggcggag cgatataggt cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaag
    43921 ccgacgtgtt tgataacttc gaggatttta tgactgcttt tatcgaccag tagaatatta
    43981 ctgtgtttcc ccataatttc gataatcaag gtagcctgga tatggtctcc aatctcgttt
    44041 ttattggaaa ctgtcatttc cacaatacgg tcattttcca cttgctcaat cgactcaatc
    44101 agggccccct gcaaatactt tctcaaaacc atgataaagg tagaaggttg ggctggattt
    44161 tcaaaagtcg tttgggtcag ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg
    44221 cgatggcttt ggcgattgct gcggatttgc aagaccaact cttgttcaaa aggctgattg
    44281 attttctgga tgcgaccatt cactaattcg cttcgcaatt cctcaactat gtggtgtaaa
    44341 aaaaatccgt caaatgacat cgttctctcc ttgtgattgt attccatagt attatatcaa
    44401 aaaggtagaa taaaatcatg gaaatgtggt ataataaagc caagtaaaga gaaacgagaa
    44461 gcacatgtat attgaaatgg tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca
    44521 aacccaagaa attttggaat ttgcagccaa aaaattagga aaagaagaca aggagatggc
    44581 agtcactttt gtgaccaatg agcgtagtca taaacttaat ctggagtacc gtgacaccga
    44641 ccgtccgaca gatgtcatca gccttgagta taaaccagaa ttggaaattg cctttgacga
    44701 agaggatttg cttgaaaatc cagaattggc agagatgatg tctgagtttg atgcctatat
    44761 tggggaattg ttcatctcta tcgataaggc tcatgagcag gccgaagaat atggtcacag
    44821 ctttgagcgt gagatgggct tcttggcagt acacggcttt ttacatatta acggctatga
    44881 tcactatact ccggaagaag aagcggagat gttcggttta caagaagaaa ttttgacagc
    44941 ctatggactc acaagacaat aaacgaaaat ggaaaaatcg tgacctgata tccagtttag
    45001 aatttgccat cacagggatt tttactgcta tcaaggaaga acgcaatatg cgaaaacatg
    45061 cagtgacggc tctagtggtc atccttgcag gttttggttt tcaggtgtca cgaatcgaat
    45121 ggctctttct cctattgagc attttcttgg tggtagcctt tgaaattatc aattctgcta
    45181 ttgaaaatgt ggtggatttg gccagtcact atcacttttc catgctggct aaaaatgcca
    45241 aggatatggc ggctggcgcg gtattagtgg tttctctttt tgcagcctta acaggcgcat
    45301 tgatttttct cccacgaatc tgggatttat tattttaaac agtaagagga aattatgact
    45361 tttaaatcag gctttgtagc cattttagga cgtcccaatg ttgggaagtc aaccttttta
    45421 aatcacgtta tggggcaaaa gattgccatc atgagtgaca aggcgcagac aacgcgcaat
    45481 aaaatcatgg gaatttacac gactgataag gagcaaattg tctttatcga cacaccaggg
    45541 attcacaaac ctaaaacagc tctcggagat ttcatggttg agtctgccta cagtaccctt
    45601 cgcgaagtgg acactgttct tttcatggtg cctgctgatg aagcgcgtgg taagggggac
    45661 gatatgatta tcgagcgtct caaggctgcc aaggttcctg tgattttggt ggtgaataaa
    45721 atcgataagg tccatccaga ccagctcttg tctcagattg atgacttccg taatcaaatg
    45781 gactttaagg aaattgttcc aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg
    45841 gatattttga gtgaaaatct ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca
    45901 gaccatccag aacgtttctt ggtttcagaa atggttcgcg agaaagtctt gcacttaact
    45961 cgtgaagaga ttccgcattc tgtagcagta gttgttgact ctatgaaacg agacgaagag
    46021 acagacaagg ttcacatccg tgcaaccatc atggtcgagc gcgatagcca aaaagggatt
    46081 atcatcggta aaggtggcgc tatgcttaag aaaatcggta gcatggcccg tcgtgatatc
    46141 gaactcatgc taggagacaa ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg
    46201 cgcgataaaa agctagattt ggctgacttt ggctataatg aaagagaata ctaagtagag
    46261 gtaggctcat gcctgcttct tgtttttaca gaaggaggac ttatgcctga attacctgag
    46321 gttgaaaccg tttgtcgtgg cttagaaaaa ttgattatag gaaagaagat ttcgagtata
    46381 gaaattcgct accccaagat gattaagacg gatttggaag agtttcaaag ggaattgcct
    46441 agtcagatta tcgagtcaat gggacgtcgt ggaaaatatt tgctttttta tctgacagac
    46501 aaggtcttga tttcccattt gcggatggag ggcaagtatt tttactatcc agaccaagga
    46561 cctgaacgca agcatgccca tgttttcttt cattttgaag atggtggcac gcttgtttat
    46621 gaggatgttc gcaagtttgg aaccatggaa ctcttggtgc ctgacctttt agacgcctac
    46681 tttatttcta aaaaattagg tcctgaacca agcgaacaag actttgattt acaggtcttt
    46741 caatctgccc ttgccaagtc caaaaagcct atcaaatccc atctcctaga ccagaccttg
    46801 gtagctggac ttggcaatat ctatgtggat gaggttctct ggcgagctca ggttcatcca
    46861 gctagacctt cccagacttt gacagcagaa gaagcgactg ccattcatga ccagaccatt
    46921 gctgttttgg gccaggctgt tgaaaaaggt ggctccacca ttcggactta taccaatgcc
    46981 tttggggaag atggaagcat gcaggacttt catcaggtct atgataagac tggtcaagaa
    47041 tgtgtacgct gtggtaccat cattgagaaa attcaactag gtggacgtgg aacccacttt
    47101 tgtccaaact gtcaaaggag ggactgatgg gaaaaatcat cggaatcact gggggaattg
    47161 cctcaggtaa gtcaactgtg acaaattttc taagacagca aggctttcaa gcagtggatg
    47221 ccgacgcagt cgtccaccaa ctacagaaac ctggtggtcg tctgtttgag gctctagtac
    47281 agcactttgg gcaagaaatc attcttgaaa acggagaact caatcgccct ctcctagcta
    47341 gtctcatctt ttcaaatcct gaagagcaaa aatggtctaa tcaaattcaa ggggagatta
    47401 tccgtgagga actggctact ttgagagaac agttggctca gacagaagag attttcttca
    47461 tggatattcc cctacttttt gagcaggact acagcgattg gtttgctgag acttggttgg
    47521 tctatgtgga ccgagatgcc caagtagaac gcttaatgaa aagggaccag ttgtccaaag
    47581 atgaagctga gtctcgtctg gcagcccagt ggcctttaga aaaaaagaaa gatttggcca
    47641 gccaggttct tgataataat ggcaatcaga accagcttct taatcaagtg catatccttc
    47701 ttgagggagg taggcaagat gacagagatt aactggaagg ataatctgcg tattgcctgg
    47761 tttggtaatt ttctgacagg agccagtatt tctttggttg taccttttat gcccatcttc
    47821 gtggaaaatc taggtgtagg gagtcagcaa gtcgcttttt atgcaggctt agcaatttct
    47881 gtctctgcta tttccgcggc gctcttttct cctatttggg gtattcttgc tgacaaatac
    47941 ggccgaaaac ccatgatgat tcgggcaggt cttgctatga ctatcactat gggaggcttg
    48001 gcctttgtcc caaatatcta ttggttaatc tttcttcgtt tactaaacgg tgtatttgca
    48061 ggttttgttc ctaatgcaac ggcactgata gccagtcagg ttccaaagga gaaatcaggc
    48121 tctgccttag gtactttgtc tacaggcgta gttgcaggta ctctaactgg tccctttatt
    48181 ggtggcttta ttgcagaatt atttggcatt cgtacagttt tcttactggt tggtagtttt
    48241 ctatttttag ctgctatttt gactatttgc tttatcaagg aagattttca accagtagcc
    48301 aaggaaaagg ctattccaac aaaggaatta tttacctcgg ttaaatatcc ctatcttttg
    48361 ctcaatctct ttttaaccag ttttgtcatc caattttcag ctcaatcgat cggccctatt
    48421 ttggctcttt atgtacgcga cttagggcag acagagaatc ttctttttgt ctctggtttg
    48481 attgtgtcca gtatgggctt ttccagtatg atgagtgcag gagtcatggg caagctaggt
    48541 gacaaggtgg gcaatcatcg tctcttggtt gtcgcccagt tttattcagt catcatctat
    48601 ctcctctgtg ccaatgcctc tagccccctt caactaggac tctatcgttt cctctttggc
    48661 ttgggaaccg gtgccttgat tcccggggtt aatgccctac tcagcaaaat gactcccaaa
    48721 gccggcattt cgagggtctt tgcctttaat caggtattct tttatctggg aggtgttgtt
    48781 ggtcccatgg caggttctgc agtagcaggt caatttggct accatgctgt cttttatgcg
    48841 acaagccttt gtgttgcctt tagttgtctc tttaacctga ttcaatttcg aacattatta
    48901 aaagtaaagg aaatctagtg cgagtaaaaa ttaatctcaa atgctcctct tgtgacagta
    48961 tcaattacct aaccagtaaa aattcaaaaa cccatccaga caagattgag gttttaaagt
    49021 attgtcccaa ggaaagaaaa gtaaccctac atcttgaatc taagtagatt ttatggtaaa
    49081 ataataggga ttttaaggag tttgatatgt ataacctatt attaaccatt ttattagtat
    49141 tatctgttgt gattgtgatt gcaattttca tgcaaccaac caaaaaccaa tccagcaatg
    49201 tatttgatgc cagttcaggt gatttgtttg aacgcagtaa agctcgcggt tttgaagctg
    49261 taatgcagcg tttgacaggg attttagtct ttttctggct agccattgcc ttagcattga
    49321 cggtattatc aagtagataa gaaaataatg ggcaggacta ggtctttgcc tctttttatt
    49381 ttt
//
LOCUS       DAVLFB010000004        35072 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLFB010000004 DAVLFB010000000
VERSION     DAVLFB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.004"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(117..1136)
                     /gene="adhP"
                     /locus_tag="VXD58_000208"
     CDS             complement(117..1136)
                     /gene="adhP"
                     /locus_tag="VXD58_000208"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV0674969.1"
                     /translation="MKAVVVNPESTGVAIKEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWAVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1488..2300)
                     /locus_tag="VXD58_000209"
     CDS             complement(1488..2300)
                     /locus_tag="VXD58_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0674970.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2490..3908
                     /locus_tag="VXD58_000210"
     CDS             2490..3908
                     /locus_tag="VXD58_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV0674971.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            3961..4668
                     /locus_tag="VXD58_000211"
     CDS             3961..4668
                     /locus_tag="VXD58_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0674972.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            4763..5737
                     /gene="folP"
                     /locus_tag="VXD58_000212"
     CDS             4763..5737
                     /gene="folP"
                     /locus_tag="VXD58_000212"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV0674973.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALATGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5739..7061
                     /locus_tag="VXD58_000213"
     CDS             5739..7061
                     /locus_tag="VXD58_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV0674974.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            7042..7596
                     /gene="folE"
                     /locus_tag="VXD58_000214"
     CDS             7042..7596
                     /gene="folE"
                     /locus_tag="VXD58_000214"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV0674975.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7639..8451
                     /gene="folK"
                     /locus_tag="VXD58_000215"
     CDS             7639..8451
                     /gene="folK"
                     /locus_tag="VXD58_000215"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV0674976.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8496..8813)
                     /locus_tag="VXD58_000216"
     CDS             complement(8496..8813)
                     /locus_tag="VXD58_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV0674977.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9170..9616
                     /gene="rplM"
                     /locus_tag="VXD58_000217"
     CDS             9170..9616
                     /gene="rplM"
                     /locus_tag="VXD58_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV0674978.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9635..10027
                     /gene="rpsI"
                     /locus_tag="VXD58_000218"
     CDS             9635..10027
                     /gene="rpsI"
                     /locus_tag="VXD58_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV0674979.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10289..10711)
                     /locus_tag="VXD58_000219"
     CDS             complement(10289..10711)
                     /locus_tag="VXD58_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0674980.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            10858..11034
                     /locus_tag="VXD58_000220"
     CDS             10858..11034
                     /locus_tag="VXD58_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674981.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11436..13649
                     /locus_tag="VXD58_000221"
     CDS             11436..13649
                     /locus_tag="VXD58_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV0674982.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(13871..14347)
                     /locus_tag="VXD58_000222"
     CDS             complement(13871..14347)
                     /locus_tag="VXD58_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV0674983.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQAQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            14570..17773
                     /locus_tag="VXD58_000223"
     CDS             14570..17773
                     /locus_tag="VXD58_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV0674984.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKGG
                     AITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYYWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            18153..18538
                     /gene="tnpA"
                     /locus_tag="VXD58_000224"
                     /pseudo
     CDS             18153..18538
                     /gene="tnpA"
                     /locus_tag="VXD58_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(18917..19546)
                     /locus_tag="VXD58_000225"
     CDS             complement(18917..19546)
                     /locus_tag="VXD58_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV0674985.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(19556..20557)
                     /locus_tag="VXD58_000226"
     CDS             complement(19556..20557)
                     /locus_tag="VXD58_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV0674986.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(20588..21229)
                     /locus_tag="VXD58_000227"
     CDS             complement(20588..21229)
                     /locus_tag="VXD58_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV0674987.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(21248..22063)
                     /locus_tag="VXD58_000228"
     CDS             complement(21248..22063)
                     /locus_tag="VXD58_000228"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV0674988.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            22334..22768
                     /locus_tag="VXD58_000229"
     CDS             22334..22768
                     /locus_tag="VXD58_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0674989.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQESVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            22780..23970
                     /locus_tag="VXD58_000230"
     CDS             22780..23970
                     /locus_tag="VXD58_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV0674990.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            23981..24472
                     /locus_tag="VXD58_000231"
     CDS             23981..24472
                     /locus_tag="VXD58_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0674991.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            24487..25266
                     /locus_tag="VXD58_000232"
     CDS             24487..25266
                     /locus_tag="VXD58_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0674992.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            25253..26071
                     /locus_tag="VXD58_000233"
     CDS             25253..26071
                     /locus_tag="VXD58_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0674993.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGENQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            26071..26364
                     /locus_tag="VXD58_000234"
     CDS             26071..26364
                     /locus_tag="VXD58_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0674994.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            26386..28287
                     /locus_tag="VXD58_000235"
     CDS             26386..28287
                     /locus_tag="VXD58_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV0674995.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMSFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            28347..29348
                     /locus_tag="VXD58_000236"
     CDS             28347..29348
                     /locus_tag="VXD58_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0674996.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKYTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(29458..29793)
                     /locus_tag="VXD58_000237"
     CDS             complement(29458..29793)
                     /locus_tag="VXD58_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674997.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(29804..30046)
                     /locus_tag="VXD58_000238"
     CDS             complement(29804..30046)
                     /locus_tag="VXD58_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0674998.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(30062..30256)
                     /locus_tag="VXD58_000239"
     CDS             complement(30062..30256)
                     /locus_tag="VXD58_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0674999.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            30422..31372
                     /gene="rsmH"
                     /locus_tag="VXD58_000240"
     CDS             30422..31372
                     /gene="rsmH"
                     /locus_tag="VXD58_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV0675000.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPEGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            31384..31701
                     /gene="ftsL"
                     /locus_tag="VXD58_000241"
     CDS             31384..31701
                     /gene="ftsL"
                     /locus_tag="VXD58_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV0675001.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            31705..33957
                     /gene="pbpX"
                     /locus_tag="VXD58_000242"
     CDS             31705..33957
                     /gene="pbpX"
                     /locus_tag="VXD58_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV0675002.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            33959..34939
                     /locus_tag="VXD58_000243"
     CDS             33959..34939
                     /locus_tag="VXD58_000243"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV0675003.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT        10581 a         6295 c         7784 g        10412 t
ORIGIN      
        1 ttatttttgt atgttgatat gttgagtttt tgaggagtaa tcttgaattc ttatttaaaa
       61 tttaatataa aaaagttgga agaagcttcc aacttttaaa ataggtttct attagattag
      121 tgggtgaagt cgagtaccat acgtccttgg atttggcctt tttccatttc gtcgaaaatg
      181 gcaacagcat cttctactgg acgtttttga acaactggga ctaccagacc ttctgcacca
      241 aattggaagg cttcttctaa gtctttacga gttccgacaa gagaaccgat gacttggatt
      301 ccatcgagga ctgttttaac gatgctgagt tccatcattt cagaaggaag accaacagcg
      361 acgacgcgac caccagcacg aatggagtca acagcctggt tgaaggcaac tttagacaca
      421 gcagttacga cagctgaatg agctcctcca tcagtttttt ctttaatgag tccagctaca
      481 tcttcaactt cgaggccgtt aatcacaatg tcagcgccta cttcttttgc aagggcaagt
      541 ttgtcattgt tgatatcgac tgcgataaca tgagcattga atactttttt agcgtattga
      601 acagcgaggt taccaagtcc accagcaccg taaagaacag cccattggcc tggttcaact
      661 tttgcttctt tgatagcttt ataggttgtt actccagcac atgtgataga agaagcttgg
      721 gctggatcaa gtccgtcagg aactttgaca gcatagtcag cagttacgat acattgttca
      781 gccataccac cgtctactga gtagccagca tttttcactg tacggcaaag ggtttcgcga
      841 ccagttgtac agtattcgca agtgccacat ccttcaaaga accaagcaac gctgacgcgg
      901 tcaccgactt taaggctttt cacatctgga gcaatctctt taacgatacc gataccttcg
      961 tgcccaagaa cacgtcctgg gacttgacca aagtcaccat gagcaacgtg gaggtcggtg
     1021 tggcaaacgc cacagtattc aacttctaca agtgcttccc cagtttcaag tggacggagt
     1081 actttttctt taatagcaac accagtgctt tctggattta caacaacagc tttcatagct
     1141 atcctccttg atattgaccg agaacaggga caacgggact ggattgattt tttgtcaaca
     1201 gagtcgagtg tgacgagctc tcttgtattt atatgtgaag tatatcacaa aagaaagcct
     1261 tttccaaggt tttggagaca aaaagtagaa caatcgatta actggttgat gagactgtga
     1321 aaagagcaca aagaccagct tgcaagctgg tcatactctt cgaaaatctc ttcaaaccac
     1381 gtcagcttca tctacaacct caaagcagtg ctttgagcaa cctacggcta gcttcctagt
     1441 ttgctctttg attctcattg agtatcagta taggttataa gaataagtta taagaataag
     1501 tcttgtaggg ttttggcaac cccatcttgg tcgttggtaa gggaaatttg ctcatctgca
     1561 taagggagta gctctggatt ggcatttttc atggcataac ccttcccagc aaaagcgagc
     1621 atttcggtat cattgtgttc atctccaaag gcaatcaaat cttttttgtc acgattcatt
     1681 atcttgagca agtagtccaa agcaaaggcc ttgttgacac cttttggggt acattcaagg
     1741 atattgagcg gacctcccca ggtattgata gaaagttgat gctggtagaa ggcgttcatt
     1801 tcttttgcca aggcatattt gtcactggct ctggtctgca agaggataca gttagggtcc
     1861 ttggtcacca attcaggctg gaattgatct tcaggctgga aagcttctac accaaatagt
     1921 ttgggattgg caatttcttc attgggattt gtaatgtaga attttttacg atattctcca
     1981 gcgataaaat cggcttgaat gtcctctgaa cgttgaacca tatctagcag atattttttg
     2041 tctacagtca aacacttttc aaaatcccaa acttggtctg gtaaatgagt aagggatccg
     2101 ttgaagttaa tcataggagt gtctaagccc agttcacggt aaaaatcttt tgacatacgg
     2161 taagggcgac ctgtcgtaat aataacctga tggccttttt cagcaacttt tttgatggtt
     2221 tttttggtaa agtcagaaat ttgactgtct gagttgagca gggttccatc taggtcaact
     2281 gcaataattt ttttcgtcat atggaccttt ctagtgtgtt ttcagataag atgtcttcta
     2341 ttataacaga aagcaggcag aaaagcagat tccaaacaaa aaatgaaatt ggattaattt
     2401 cttaaacaaa tcaaacaaag acattgaaaa agtgaatgat aaatgatata attctattat
     2461 tgttcgtaaa aattaaaagg agattgatga tggacaaatt atttaaacta aaagagaacg
     2521 gtacagacgt tcgtacagag gttctcgctg gtttaacaac tttctttgca atgagctata
     2581 ttctctttgt aaacccacaa attctttcgc aaacaggaat gcctgctcag ggtgtgtttc
     2641 ttgcaacgat tatcggtgca gttgctggta ccttgatgat ggccttctat gctaacttgc
     2701 catacgctca agcaccaggt atgggactca atgccttctt tacctttaca gtcgtatttg
     2761 gacttggtta ttcatggcaa gaagccctag ctatggtctt catctgtggg attatttcat
     2821 tggttattac cttgacaaat gttcgtaaaa tgatcattga atcgattcca aatgcccttc
     2881 gctcggctat ttcagctggt atcggtgtct tccttgccta tgtagggatt aagaatgctg
     2941 gacttttgaa atttacgatt gatccaggca actatactgt tgtaggagaa ggggctgaca
     3001 aagctcaagc aacgattgca gcaaactctt cagcagttcc aggcttggtc agctttaata
     3061 atccagctgt tttagtggct cttgcaggac ttgccattac tatcttcttt gtcatcaaag
     3121 ggattaaagg gggaattatt ctctctatct tgacaacaac tgttcttgct attgcagttg
     3181 gtttggttga tttgtctagt atcgattttg ctaataacca tgttggtgca gcttttgaag
     3241 atttgaagac aatctttggt gcagctcttg gttcagaagg tttgggagct ttggtttcag
     3301 atacagctcg cttgcctgaa actctgatgg ccattcttgc cttctcattg acagatattt
     3361 ttgacacaat tggtaccttg atcggtacag gtgaaaaagt tggtattgta gcgacaaatg
     3421 gtgaaaatca ccaatcagcc aaattggata aggctcttta ctctgatttg attggaacga
     3481 cagtcggtgc cattgcaggt acttcaaacg taacgactta tgttgagtct gctgctggta
     3541 tcggtgcagg tggacgtact ggtttgacag ccttggttgt agctatctgt tttgcgattt
     3601 caagcttctt tagcccactt ctagcgatcg taccaacagc ggctacagct ccaatcttga
     3661 ttatcgttgg gattatgatg cttggtagct tgaaaaatat ccattgggat gatatgtctg
     3721 aagcagttcc tgccttcttc acatctatct ttatgggatt cagctactct atcactcaag
     3781 ggattgcagt tggtttcttg acttacactt tgactaagct tgtcaaaggt caagttaaag
     3841 atgttcatgc catgatttgg attttggatg ccttgtttat ccttaactac atcagcatgg
     3901 ccttataata gaatgaccca gggggatttc cccccttttt taatacaaag gagataggtg
     3961 atgaaagaga aaaatatgtg gaaagaattg ttgaatcgtg caggctggat tttggtcttt
     4021 ttacttgccg tccttttata tcaggttccc ctagtggtta cctctatttt gactttaaaa
     4081 gaagtagccc tgctacagtc agggctgata gttactggcc tttcaattgt ggttctggct
     4141 ctatttatta tgggagctcg taaaaccaag ttagctagtt ttaatttttc tttttttaga
     4201 gctaaagatt tggcacgttt gggcttgagt tatttagtta ttgtcgggtc aaatatactt
     4261 ggttctatct tgttgcaact gtcaaatgag acgacaacag ctaaccagtc tcagattaac
     4321 gatatggttc agaatagttc tctgatttct agcttcttct tgctagcctt gcttgctcca
     4381 atttgtgagg aaatcttgtg ccgtgggatt gttcctaaaa agattttccg aggcaaggag
     4441 aacttgggat ttgtagtcgg tacgattgtg tttgctttat tgcatcaacc aagtaattta
     4501 ccttctttat tgatttatgg aggtatgtcg acagttctat cttggacggc ctacaagacc
     4561 caacgtttgg aaatgtcgat cttgcttcac atgattgtta atgggattgc tttctgtttg
     4621 ttggctcttg tggtgattat gagtcggaca ttaggaattt ctgtttaaaa gtttttatgt
     4681 aggaaccgac ctctttctac tagggaaaga tgaatgcaat cgtgtccatc tttttctttt
     4741 tatggtaaaa tagaaaaata atatgatgaa aatccttgag ggagtgaccg atatgtcaag
     4801 taaagccaat catgcaaaga cagttatttg cggaattatc aatgtaaccc cagactcctt
     4861 ttcggacggt ggtcaatttt ttgctcttga gcaggcactc cagcaggctc gtaaattgat
     4921 agcagaagga gccagtatgc tcgatatcgg cggagaatcg actcggccgg gaagtagcta
     4981 tgttgagata gaagaggaaa tccagcgtgt tgttccagtg atcaaagcga ttcgcaagga
     5041 aagtgatgtc ctcatctcta ttgatacttg gaagagtcaa gtagcagagg ctgctttggc
     5101 tactggtgcc gatctagtca atgatatcac tggtctcatg ggtgatgaaa aaatggccca
     5161 tgtggttgct aaagctggag cgaaagtggt catcatgttt aacccagtta tggctcgacc
     5221 tcagcatcct agttcgctta tcttccctca ttttggcttt ggtcaaacct ttacagaaaa
     5281 agagttagct gactttgaaa cattgccaat cgaagacttg atggtggctt tctttgaacg
     5341 agcactagcg agagcggcag aagctggtat tgcaccagaa aatatcctgt tggatccagg
     5401 aattggcttt ggtctgacca agaaagaaaa tcttctcctt ttacgggacc tggataaact
     5461 acatcagaag ggctatccaa tctttctcgg agtgtcgcgc aagcgatttg tcatcaatat
     5521 ccttgaagaa agtgggtttg aagtcaatcc tgagacagag cttggtttcc gaaatcggga
     5581 cacggcttcg gctcatgtaa ctagtatcgc tgcgagacag ggtgtagaag tggtgcgcgt
     5641 gcatgacgta gctagtcaca ggatggcagt tgaaattgcc tctgccattc gtctggctga
     5701 tgaagcggaa aatttagatt taaaacaata taaataagat gaaagaaatt gaaaacaatc
     5761 agtggattgc taactaccgg acggatcaac cgcattttgg cttggaacga atggtggaac
     5821 tgttagcttt gcgtggcaat ccccatctta aactcaaggt tctccatatc ggagggacta
     5881 acggcaaggg ctcgactatt gcttttttga aaaagatgct agaaaagcta gggctgagag
     5941 ttggcgtgtt tagttcgccc tatctcattc attacacaga ccagattagc atcaatgggg
     6001 aatcgatctc agaagcgagg ctagaagctc tcatggcaga ctatcagtct ttgctggagg
     6061 gagaagcggt cgccaattta cagggcacaa ccgagtttga gattatcaca gccctagcct
     6121 atgactactt tgcctcagag caagtagatg tggctatcat ggaagttggc atgggtggac
     6181 ttttggatag taccaatgtc tgtcagccca ttttgacagg aattacaact attggcttgg
     6241 atcatgtggc cctacttggt gacaccttgg aggccatagc agagcagaag gcaggtatta
     6301 tcaaacaagg gatgcccttg gtaacagggc gtattgctcc agaagccttg actgtgattg
     6361 accgcattgc ggaagggaaa gatgcgccga gacttgccta cgggacagat tatcaggttc
     6421 gtcatcaaga aagtgtggtg acaggggaag tctttgacta tacaagtgct gtcagacaag
     6481 gtcgcttcca gactagcctg cttggtttgt accaaataga gaatgctggg atggccatag
     6541 ctttacttga tactttttgt caagaagatg gtcgagagct agcaagcaat gactttcttg
     6601 gtcaagcctt ggaagaaaca agttggccag ggcgtttgga aatcgtgtca agagatccct
     6661 tgatgatttt ggatggagcc cataatcccc atgctatcaa ggccttgttg gtaaccttgc
     6721 aagaacgttt tgcggattat cataaggaaa tcctcttcac ttgtatcaaa accaaagcct
     6781 tgggggatat gttggacttg ctgggagcca tgccagatac cgagcttact ctaacacatt
     6841 ttgcggatag tcgggcgacg gatgaaagcg tgctgaaaga ggcagctaag tctagaaatc
     6901 tcagctacca agattggcat gattttctag agcagaattt gacagataaa aaagaagaga
     6961 aacaaacagt taggattgtc acaggttcct tgtatttctt gagccaagtg agggcctatc
     7021 tgatggagag gaagaacgag aatggataca caaaagattg aagcagctgt aaaaatgatt
     7081 atcgaggctg taggagagaa cgctaatcgc gagggcttgc aggaaacacc tgctcgtgta
     7141 gcccgtatgt atcaagagat tttttcaggt cttggtcaaa cagcagagga acatttgtca
     7201 aaatcctttg aaattattga cgataatatg gtggtagaaa aggatatctt tttccatacc
     7261 atgtgtgaac accacttctt gccattttat ggtagagcgc acattgccta cattccagat
     7321 ggtcgtgtgg caggcttgtc taagctagcc cgtacggttg aagtttattc gaaaaaacca
     7381 caaattcaag aacgtttgaa tatcgaagtg gccgatgcct tgatggacta tctaggtgct
     7441 aaaggagcct ttgttgtcat tgaggcggaa catatgtgta tgagtatgcg tggtgttaga
     7501 aaaccaggca ctgcaacctt gacgacagta gctcgtggtc tatttgaaac agataaggac
     7561 ctccgcgacc aagcttatcg tttaatgggg ctataaaaag aatccgtttc aagcggattt
     7621 ttctagaaag gaatcattat ggatcaactg cagattaagg atttggaaat gtttgcctat
     7681 catggtcttt ttcctagtga gaaagaattg gggcagaaat ttgtcgtttc agccatccta
     7741 tcctatgata tgaccaaggc agctacagac ttggatttaa cagcctctgt ccattacgga
     7801 gaattgtgtc agcagtggac gacttggttt caggaaacga gtgaagattt gattgaaacg
     7861 gtagcctata aactggtgga acgtaccttt gagtcttatc ctcttgtcca agaaatgaag
     7921 ttggaactga aaaaaccttg ggcaccggtg catttgtcac tagatacttg ctcggtaacc
     7981 attcatcgcc gcaagcaacg agcctttatc gccctaggaa gcaatatggg agataaacaa
     8041 gcaaacttga agcaagccat tgacaaactg cgagctcgtg gcatctatat tctcaaagag
     8101 tccagtgtct tagcgacgga gccttggggt ggagtggagc aggatagctt tgccaatcaa
     8161 gtggttgagg tggaaacctg gctaccagca caagacttgt tagaaacctt gttagccatt
     8221 gagtcagagc tgggacgggt gagagaagtg cattggggac ctcgtttgat tgatttggac
     8281 ttgctctttg tggaggacca gatcctttat acagacgacc tcatattgcc tcatccttac
     8341 atagcggaac gcctttttgt ccttgagtcc ttacaggaaa ttgcgcctca ttttatccat
     8401 ccgatattaa aacaaccgat ccgtaacttg tatgatgctt tgaaaaaata gaaaaactct
     8461 agttttcagt tacttgcaac tgaaggctag agtttttaaa ataggtcatt ttcttctggg
     8521 aggaggatag tttctctacc gtccatgtct aaaaccagta ctcttggggg ataacgaggg
     8581 tcgaaaggat ggttaaagtc aaaatcaatg gctgtaggga ggtgttgact tgaaaagtgg
     8641 aaggtaatct ttccttggtt attaagcaat tgaaactcga gttcttcttc caattcaaag
     8701 acatttttta agaaatggtc gataatatac caaaaagagt caatgatgtc atcaggcaag
     8761 ctggtaacaa tgccaaaact agcagaccgc atgtgggtat tggtaaaagc catatctctg
     8821 tctcctttct tttcccttat catacagcaa ataggattaa aaatcaagaa aaggtgattt
     8881 tttgaaaagg attttaacta cagggaaaaa gaaggaaaaa aattcccaaa aatctaccga
     8941 agttaatagg taaattccca aattaacttg attatataac tttcagttat tttgagaagt
     9001 taccgaaaaa tattttttat atctattgac ttttaggggt gaaatttggt atgatagtag
     9061 gcggtattgt ttaccccatt tgaaaggccc cggaaccttc caaatacttt tcgatgggaa
     9121 ggaacaccca tcaccgtaaa caaaaatcga actatatata ggagaaatca tgaacaaaac
     9181 aacatttatg gctaaaccag gccaagttga acgtaaatgg tacgtagttg acgcaactga
     9241 tgtaccactt ggacgtcttt ctgcagtagt tgctagcgta cttcgcggaa aaaacaaacc
     9301 aacatttaca ccacacactg atacaggtga cttcgtgatt gttatcaatg ctgaaaaagt
     9361 taaattgact ggtaaaaaag caactgataa aatctactac actcactcaa accacccagg
     9421 tggattgaaa caaatctctg caggtgaact tcgttctaaa aatgcagtac gtttgattga
     9481 gaaatcagtt aaaggtatgc ttccacacaa tacacttgga cgcgctcaag gtatgaagtt
     9541 gaaagtattt gttggagctg agcacactca cgctgcacaa caaccagaag ttcttgacat
     9601 ttcaggactt atctaaggaa aggaacaata aagtatgtca caagcacaat atgcaggtac
     9661 tggacgtcgt aaaaacgctg ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac
     9721 tgttaacaaa aaagatgttg aagagtacat cccacacgct gaccttcgtc ttgtcatcaa
     9781 ccaaccattc gcagttactt caactgtagg ttcatacgac gttttcgtta acgttgtagg
     9841 tggtggatac gctggtcaat caggagctat ccgtcacggt atcgctcgtg cccttcttca
     9901 agtagaccca gacttccgcg attcattgaa acgcgcagga cttcttacac gtgactcacg
     9961 taaagttgaa cgtaagaaac caggtcttaa gaaagctcgt aaagcatcac aatttagtaa
    10021 acgttaattc gaaagaatta ctatacttat acagagcacc tttcggggtg ttcttttttt
    10081 atactttctt actaaattgg tgcaattgac acagttgttg cgactttagt cgcttacaaa
    10141 tgtggctgca acctgacaag gtcagttgcc tcaaaacgtt aatcaataca attatatcaa
    10201 cgtttcaaag cactcaagag tttacctcat gggtgctttt tttatgtttt ttgaaaaagt
    10261 tcacctcaaa gtttacctta ttttcacctt attgttttag agactgtaag gcaaattcac
    10321 cgacggccat agggactaca ttagaagtat tgacataaat ctgtgttgta cctgtgtcgc
    10381 tatgtattag agcttcagaa atggcttcta agctcattcc tgcttgttta gcgagcgttg
    10441 ctccggtatg acgtagtttg tgtggcgtag cgtgtgtaag ctctgggtgt cgttttctga
    10501 tagttttcat cttattattg agatagtcag aatgtaaagg tttattgata ttgcctttgg
    10561 tatctatgta agtaaagatg aattgttctg gattactgat gataccaaac tttgccagtt
    10621 catatttttg ttgttttttc catgaagtca gaagttgaag taagtcatta gaaatagaga
    10681 aaatgggaaa tgtttccaca ataaaacgca tattatcaga tggttaatgc gttttatatt
    10741 tgggtttgag gcttataaaa taggataagc gtactgaaaa cttagatatg ataaacaaaa
    10801 taaaattttt aaaagctaca tagaaacatg gtataatgta tctaaaggag gtagcatatg
    10861 tccttagata tagataaaga aaagatgaca atcatgggaa tagcttttga aaaccgatcc
    10921 gtttttaaga gtgtttggta tgctttaagt acaaatatga ttgaaggatg gcgacctacg
    10981 gttagtgatg ttgaaaaatt acgagatgaa gctcttgctc tggggatgac ttaatgaagc
    11041 gcaactgtta tgaatcttat gactatgttg atccggataa tttatatacc gatccgggtt
    11101 cttcagttct aagaaataag caggaaggaa aaaagcacgc gagcttgaat atcgtatgat
    11161 tgctagtaag agtttgaaat tatttatcaa tcctatctta gttaatagtt taaaagtagt
    11221 ttgtttcaca actgcataag cttagaaaag ttgaaaagtg ataactcttt aataaactct
    11281 tggtttgtta aaagatattt tgtccactac atttttttca attgactatt atttgtcata
    11341 gatataacag atagaacatt gcattcagaa taatgaaaaa cgcatcaaaa atggtgcgtt
    11401 ctgtttttat gctaccataa aaggaggaaa acaaattgaa aatttttaag ggagagtttt
    11461 atcgaatctc tgtattaaca gacaagctag taaggttaga atactctcga actggaaatt
    11521 ttgaggatag aacgacacaa cttatctata atagagattt tggccaagtt tcgttagatt
    11581 atatcgagac atcaaatgta ctagatatta tgacggacta ttttcatctg cactttaata
    11641 aaggagaatt taacgccgaa aatttattta tagaattaaa aggaaatttt gccgtatatg
    11701 gtagtcgctg gtattttggt gaatctattg aaacgttaaa aggaacagct cggactctgg
    11761 ataaggcaga tggagcaatc tcgttagaag atggaattat tagccgaaat ggtatagcct
    11821 tattggatga ttctcaagga tttatttggg atgaacaatc tggttatatt gagagagaaa
    11881 atcaaattga cctgtatttc tttgcctatg ggcatgatta tagaggagca atcagagact
    11941 tttaccattt gactggttca acacccttgt tgccaagata tgctttaggc aattggtgga
    12001 gtagatattg gccttatacg tcggatgaat acttggattt aatagacaga tttgaaacag
    12061 agaaaattcc attatctatc ggtgtgttag atatggattg gcatataact gacattccag
    12121 ctcgttttgg aagtggctgg acaggatata gttggaatag aaacttaata ccaaatccag
    12181 aacagttatt gcaacaactt catgatagaa agttaaaact ctccttaaat gtccatcctg
    12241 ctgatgggat acgggcttat gaagaagctt atcctcaaat cgcaaaacga ttggggttaa
    12301 atgtagaact agaagaacct gctatttttg atttttttaa tccctctttt agggaagcct
    12361 actttaaaga tgttcattat gagttagaaa agcagggagt agatttttgg tggattgact
    12421 ggcaacaagg gacacaaggt atgctagatc cactttggct tttaaaccat tatcactatc
    12481 aggatagttg taaaaatgca gaaggtggtt tgattttatc aagatatgca ggtcctggta
    12541 gtcaccgcta ccctgttggt ttttcagggg atactattat tagttggaat tccttaagat
    12601 ttcaacccta ttttacagcg acagcatcta atatcggtta tagttggtgg agtcatgata
    12661 tcggtggaca tatgttgggg gattatgacg aagagctaca aactagatgg ctacagtttg
    12721 gtgtttttag tccgattact cgattacata gttctagaag tccttttaat agtaaagaac
    12781 cttggttttt ttcagaaaca acatctaaga ttatgaagaa atacctccgt ttgagacatc
    12841 agatgattcc ctatctatat accatgaatg taaagacaca tgaggaaggt gccccattaa
    12901 tcagtccaat ctattatttc tatccagaga atgatgagag ctataatgtt ccaaatcaat
    12961 acttttttgg aacagaactg atggtggctc ccattgtaga aaagatggat ttgacattcc
    13021 aatctgcaaa agtagatgta tggttccctg aaggtgaatg gtatgacttt ttttcagaga
    13081 aaaaatacac aggtggtgtg aagttaagtg tttatagaga catctcgact acgcctgtgt
    13141 ttgcaaaaag tggtgcaatc attcccttgg ttggttctga gataggtatg ggtgttgatt
    13201 tacctgaagt tgtagattgg tatgtattcc caggaaaaca acattctttt gaaatgcttg
    13261 aagatcaaaa tggtcaaaga tataaaacaa gattatcaat cgattgggaa atgggaatgg
    13321 tagagttagc attacaagga gattctagta tcgttccaag caatagaaaa catagaattc
    13381 attttaaagg aacgaatgtg tctataattg aattgccaaa taagaatgat acagctaaat
    13441 ttgaatggaa agataataaa aggacatctt taaatgacga agtttttaga ctactaaaga
    13501 cagcttctct tccatatgaa ttaaaagata gattgttaaa tcaattcatc aatgcgaaaa
    13561 actctcatga cttaatgaat atcttgcatc atcaggataa ggaattgaga gggcgtttgt
    13621 tggaaatgat atttactagc caaaactaat tgaaataatt agaaagtcat ttgtttagtg
    13681 tatatgaaga ttctgtatgg tattcgttat cactggtcac attgaaaatg tgaaataagc
    13741 acctacaaca tagactttac aacacttcat gttttatatc atggggtgtt tttctattta
    13801 agtcaaagcc catcaaaaaa tctcactatt catcaggtta aaggaaacct aatcatagtg
    13861 agattgtgaa ttatagtaga gtttgtatcg cctcttcaat ttgttttggg tctgtttttg
    13921 aagaaaagcg ttcaaagact tgcccatctc gaccgatgag aaacttagag aaattccatt
    13981 cgattcgttt tcctagtggg ccggatttct ggtcttttaa ccagacatag agagggtctg
    14041 cttccttacc gttgacctta atcttggcaa aacgtgggaa ggtggtttga aaatgtaggc
    14101 tacagaaggc gttgatttcc tctgcgctgc cgggtgcttg tcccataaac tgattgcaag
    14161 ggaaatccaa tatttcgaag ccctgagctt gatagcgttc atagagttct tgaagtccct
    14221 ggtactgggg cgttaaacca catccagtag cagtgttgac aatcaagaga actttgccac
    14281 gatagctatc caagggagtt gcttggttgt tttggttcaa aacggaaaaa tcataaagtg
    14341 aagtcatgag ggcctctttt ctttttctac attgtaacag tttttacttt tttcgtcgag
    14401 tcggatggaa gatattgttg aagaataagt cttcaatatg atagaaaccg tttacaaata
    14461 actttaaaaa tggtatgatt taattaaacc ggtttactta gttgctataa atctctaatt
    14521 tgagatttag ttcctattga ttttacaata tgtttattgg agtgtataca tgcaaacaaa
    14581 aacaaagaag ctcattgtga gtttgtcttc acttgtttta tcaggatttt tattaaacca
    14641 ttatatgaca gttggagcgg aagaaacgac tacgaatacc attcagcaaa gccagaagga
    14701 agttcagtat cagcaaaggg atacaaaaaa tttagttgaa aatggtgatt ttggtcagac
    14761 ggaggacgga agcagtccgt ggacaggaag caaagctcag gggtggtcag cttgggtaga
    14821 ccagaagaat agttctgcag atgcctcaac tcgagtcatt gaggctaagg gtggggctat
    14881 cactatctca agccatgaga aattaagggc agcggttcac cgtatggttc ctattgaagc
    14941 taagaaaaag tataaactgc gtttcaagat taaaacagat cataaagtcg ggattgccaa
    15001 ggttcgtatc attgaggaaa gtggtaagga caagcgattg tggaattctg caacgacgtc
    15061 aggaacaaag gactggcaga ccattgaagc agactatagc ccgactttag atgttgataa
    15121 aatcaagctg gagttattct atgaaacagg aactgggact gtttccttta aggatattga
    15181 gctggtagag gtagcagacc agctttctga ggattctcaa acagataaac agcttgagga
    15241 aaagattgat ttaccaattg gaaaaaaaca tgttttttct cttgcggact atacttataa
    15301 ggtagaaaat cctgacgttg cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg
    15361 gacaaccaat gtcattgtca gtaaagatgg caaggaagtg aaaaagattc ctttgaagat
    15421 tctagcctct gttaaggatg catacacagc ccgtttggat gactggaatg gcatcatcgc
    15481 tgggaatcaa tactatgatt ctaaaaatga acagatggcc aaattaaacc aggaattgga
    15541 aggaaaggta gctgatagcc tatctagtat ttcaagtcag gcggaccgca cctatttgtg
    15601 ggaaaaattt tcaaattata aaatgtctgc aaatctgact gccacttatc ggaaattgga
    15661 ggagatggcc aagcaagtga ccaatccttc ttctcgttat tatcaagatg aaactgtcgt
    15721 tcgaacagtc agggattcca tggaatggat gcataaacat gtctacaata gtgaaaagag
    15781 cattgttggg aactggtggg attatgaaat cggtacacct cgtgccatca acaatacctt
    15841 gtctctgatg aaagaatact tctctgatga ggaaattaaa aaatatacag atgtgattga
    15901 aaaatttgta ccagatcccg aacatttccg aaagacgact gataacccat ttaaggctct
    15961 aggtggaaac ttagttgata tgggaagagt aaaagtaata gctggtttac tgcgtaagga
    16021 tgatcaagaa atttcttcta ccattcgctc gattgagcaa gtgttcaagt tggtagacca
    16081 aggtgaaggt ttttatcaag atggatccta tatagaccat accaatgttg cctatacggg
    16141 tgcttatggg aatgttttga ttgatggcct gtctcaactg ttgccagtca ttcaaaagac
    16201 caagaatcca atcgataaag ataaaatgca aaccatgtac tactggattg ataaatcgtt
    16261 tgctcctttg ctggtgaatg gagagctgat ggatatgagt cgtggacgct cgatcagtcg
    16321 tgcaaatagc gaggggcacg tggccgcagt agaagtacta agagggattc accgaatagc
    16381 ggatatgtct gaaggagaaa ccaaacaacg tttgcagagt cttgtgaaga ccattgttca
    16441 atcggatagt tattatgatg tctttaagaa tttgaagact tataaggata tcagtttgat
    16501 gcaatccttg ttaagtgatg caggagtcgc aagtgttcca agaacaagtt acctatctgc
    16561 ctttaacaag atggataaaa cagccatgta caatgcagag aaagggtttg gatttggctt
    16621 gtcactcttt tccagtcgta ccttgaatta cgaacacatg aacaaggaaa ataaacgtgg
    16681 ttggtatacg agtgatggga tgttctatct ttacaatggc gatttgagtc actatagcga
    16741 tggctactgg ccaacagtta atccatataa gatgcctggt acaacagaga cggatgctaa
    16801 gagagcggat agcgatacag gtaaagtttt accgtctgct ttcgttggaa cgagcaaact
    16861 agatgatgcc aatgcgacag caaccatgga tttcaccaac tggaatcaaa cattgactgc
    16921 tcataagagc tggtttatgc taaaggataa gatcgccttt ttaggaagca atatccaaaa
    16981 cacttcaaca gatactgctg caactacaat tgaccagaga aaactggaat caagtaatcc
    17041 atataaagtc tatgtcaatg ataaagaagc ctcccttaca gaacaagaaa aggattatcc
    17101 tgaaacccaa agtgtctttt tagaatcatc cgattcgaaa aagaatattg gttacttttt
    17161 ctttaagaag agttcaatca gtatgagtaa ggctttgcaa aagggagcct ggaaggatat
    17221 caatgaagga cagtcagaca aggaagttga aaatgaattt cttacgatta gtcaggctca
    17281 taagcaaaat ggagattctt atggctatat gctcatccct aacgtgggtc gtgccacctt
    17341 caatcaaatg ataaaagagt tagaaagtag cctcatcgaa aataacgaaa cccttcagtc
    17401 tgtttatgat gccaaacaag gagtttgggg cattgtgaaa tatgatgatt ctgtctctac
    17461 tatttccaac caattccaag ttttgaaacg tggagtctat accattcgaa aagaagggga
    17521 tgaatataag attgcctact ataatcctga aacccaggaa tcagctccag atcaggaagt
    17581 ctttaaaaag ctagagcaag cagctcagcc acaagtacag aattcaaaag aaaaggaaaa
    17641 atctgaagag gaaaagaacc attcggatca aaagaatctc cctcagacag gagaaggtca
    17701 gtcaatcttg gcaagtctag ggttcttgct acttggggcg ttttatttat tccgtagagg
    17761 aaagaacaac taatttgttc atcatctaga tgaattacat gaaactgtgg aaaatagaaa
    17821 aaatccgaag aaggccaaat atcttcggat ttttctttta aaaaaggata agttgatact
    17881 tatccttttt acttcattat gtgactatag cgttttgaaa ctagaatagg acatcataac
    17941 tgctaaaaga tttctataaa ttcatttgat tttcctaatc aatttgttcg tatcctattt
    18001 cactctacta tattagtccg tctcgcacct cacggagcga gacaaaacaa ccacccgcta
    18061 tgcgggtgcg cgtcgaaggt tataccaaaa aaactccaaa cgcgatacaa taaaggtgtt
    18121 caacccactt gtaaagcgaa aggagaaaaa atatggcaca aaaggctcat agtttatctc
    18181 acacaaagtg gaggtgttct atcacattgt gttcacccct aagtatagac gaaaagtcat
    18241 ctataatcaa tatagaagta gtttaggcga aatatttcat cgcttgtgta gttataaagg
    18301 tgttgaaatg atcgagggtc acttaatgcc agaccatgta cacatgttag tcagtattcc
    18361 actaaggcta agtgtttcga gtttcatggg ttatttaaaa ggcaaaagtg cactcatgat
    18421 gtttgacaaa cacgccaatc tcaagtacaa gtttgggaat cggcatttct gggcggaagg
    18481 ttattatgta agtccagtag ggcttaatga agacacaatt aagaaatata gtcaagatta
    18541 aagaaattat cgagtggatg atttcttcac gagtatgaaa atttgagaac tagtaaagta
    18601 tgatatatag taaaatgaaa taagaacagg acaaaatcga tcaggacagt caaatcgatt
    18661 tttaacaatg ttttagaagt agaggtgtac tattccagtt tcaatctact atagcactag
    18721 ataaattgag tgtaaaagaa tatgaggatc cctttaggga tagtggtaag taataccaaa
    18781 gtctcttaaa gaggcaagtg acgagtcaag agcaataagg cttgaacaac gtgaaagcca
    18841 gcgtctttag gcgctggctg atgatttggg cttatagctc tgagataaac cacccgttag
    18901 acgggtagtt atgattttat ctgagtgtaa catactgttg ggcaatctcg ctgatgcggt
    18961 caaagttgcc ttgggaagcg agtttattga gttcgccacc aattccaacg gcatctgcac
    19021 cagcagcgaa ccattgaggg atgttgttta gaccgactcc tccggttacc attacggaaa
    19081 cttgtggaat cggtgccttg actgcagaga tatatgctgg actgagagta ctacctggga
    19141 agagtttgat gatttcacta ccggcttcaa gtgcagtcgt gatctctgtg agggtaatac
    19201 agcctggaat gtacggtgtg ctgtagagat tgcacatttt cgcagtttca gcatggaaag
    19261 atggagaaac aacgtaattt gctccggcta gaatggcatc tctagcagtt acggcatcaa
    19321 gcacagtacc tgcaccgata caaacactct gatcgtcctg atacaagtct acaagttcct
    19381 tgatgatttg tcctgcatac tgattggtat aggcgatttc aatagctttg ataccgccct
    19441 tgatacaagc aatcgaggct tgtagtcctt cttcctttgt atttcctcga atgacagcaa
    19501 caattttctg tttttttagt tcaataattg tatctgattt ggtcatgtaa ttctcctaac
    19561 gaatgatatc ttgtgcattt gccagtaaat tttcaatact agttgcggaa gtggagagat
    19621 ggtctcctgg aagagtgcat ttgagagttg cgctcgcaac tgcaaagtca atggtagttt
    19681 ttagggagga atgatggagt agttggtaaa gggcaccaga tataaaggca tccccgctac
    19741 caattcgttg atagactgca gtttttagtt ggacagactc ttcaaatcgt tcttccagag
    19801 cataggcttg atagacattt ttgtcttgct catcactaga gcggagggta tggaaaatgg
    19861 ccttgaaacc ataggcttct tttaaaagtc gcatgcgatt ttccacctct tctaggctag
    19921 cactgtctct tggaaacatc tctagatttt ggtcatcaat catgagagga tcaataccga
    19981 agcaatagtc agtaaaacgt gcaaacttag aaaattcata cttggcttct aggactgaaa
    20041 tcatctttgt tctcagattg agatccattg aaacgacaat tcctcggcgc ttggcttctt
    20101 ccaagagtag gagaaggatc gcacggacct cttgaccgat agctacggtg attccactaa
    20161 aatgaaaatg gctaatcccc tgaaagagag aatccatatc tagcatgttt ggccgaatct
    20221 ggctgatact cgtatgctta cgatcgtaga aaacttcact ttgacgacaa ccaaagccgt
    20281 tctccaaata gtagaggccg attcgatcgc caagccgaca gattgaactg gtatcgattt
    20341 ggtgctgttt caagaatgtg agaaaacgat ctccaatctc gttggcaggg agtgcggtaa
    20401 agactttcgt tgagataccc agggcttgca aattacaagc gatgttaatt tctgatccgc
    20461 caaaataagt cgaactggca acatgatctc cgatactggt ggcatctaat ggtgagattc
    20521 gaattaatgg ttcgccaaac agtaatattt tcgtcatcca ttagttcctt tctggtttag
    20581 aatagcttta cttggctaat acttctttca aataagcagc gacttcatca tcttggcagt
    20641 tttcaaagaa gtattcttgg aagtatttgc cagatatggt gtctttgagg aagtcttggt
    20701 cgattgtttt aaggatggtc atcaaatcat tgtgggtgat ttgtttcacc tcgtttaaga
    20761 tacgagcgtt gcgttgttca gggattacac gttctcttgg gtagccaccg cccatttctt
    20821 cagcaaataa gcgttcaaac atcaatttga gggtcagttc tgccccccag ccaaatcctt
    20881 tggcataagg gatagacaag gcgttaccac cattgatttg agaataaagg taagcgtcag
    20941 ttgggtccac tgctagacca cagacaacac cagggaagct gtttaaagca agcatagccc
    21001 ctacacccgt accacagccg gtaacaacaa agtcaactgc ctttgtattt aaaaggatgg
    21061 cagccattag tccgttctgc acataagtta attgactttc tccttcttct ccacgcatac
    21121 catagttaaa taattggtag tcttttttat ctgtcgcttc ttttagacta tcgtaaataa
    21181 tgtgattctt gctagcttga ctattttcat tgattaatgc gattttcata agatcttctt
    21241 tctattttta ctcaggttgt tttccgatgt aggctaagat accgccatct acatatagga
    21301 tgtggccatt gacaaaattg ctggcatcac tagcgagaaa gacagcaggg cccatcaaat
    21361 cttcaggatt tccccaacgt gcagcaggtg tttttgcaat gatgaactgg tcaaatgggt
    21421 ggcgagaacc atcttcttgc aactcacgaa gaggtgctgt ttgaggagtg gcaatataac
    21481 ccggtccaat tccgttacat tggatattgg ctccaccgta ttcagacgca atgttgcggg
    21541 tcaacatttt caagccccct ttagcagcag cataagcgct aactgtttca cgtcccagtt
    21601 cgctcatcat cgaacaaata ttgataatct ttccatgccc tttctttatc atagaaggaa
    21661 taactgcctt tgaaacgata aatggtgcgt ttaagtcaat atcgatgacc ttacggaaat
    21721 cagcggcgct catttcgcac attggaactc ggcggataat accagcgtta ttaacgagga
    21781 tgtcaatgac accaacctct tgttcgattt gcttgaccat ggcttggata ccgtcctcgt
    21841 ctgtcacgtc acagacatat ccatgggctt cgatgccaac ttcacgataa gcttcaatcc
    21901 ctttattgac cagatcttga ttgatatcgt taaagacaat agtagcgccg gcttcagcgt
    21961 aggatttggc aatagcaaat ccaattccat aagaagcgcc ggtgatgaga gcaatttttc
    22021 cttttaaaga aaactgctca attgagaatg atgtatttgt catttgatac ttcctttcaa
    22081 atttgttaag acttctctat aaccaaatta tattctagta aaccggtttt gtcaataaat
    22141 aaatattttt atttataaaa agatttattt tccttaaaaa tggaatattt tgaaatttct
    22201 gtctattaaa atggttaaaa agataatgaa agcgctaata tttttttaaa aaaactattg
    22261 acaagataaa aataaaggat tacaatgggg aatataaagt aaaccggtaa acctaaacaa
    22321 gaaaggagaa aagatgaaaa ttgtacttgt agggcatgga cattttgcta cagggattta
    22381 tagttcttta caattgattg caggcaatca agaaagtgtg gaggcgattg actttgtgga
    22441 aggaatgtca gcagatgaac tcaagcaaaa aatcttactt gcaatttcaa atgaagaaga
    22501 agttttaatc ctaagtgatc tcttgggagg atcgccattc aaggtttctt ctaccataat
    22561 gggagaaaat ccagccaaaa caatgaatgt tctctcgggt ttgaacttag ccatgttaat
    22621 ggaagcagtc tttgctagaa tggctcatag ctttgatgag gttgttaata aatcagtagt
    22681 ggcggcccag ggcggagtcg taaatggtaa agaattgttt tcaacggatg cagaggaaga
    22741 ggaagaagat ttcgaatcgg gtatttaaag ggtaaaagaa tgataaaaaa ggttacgatt
    22801 gaaaaaataa aatcgcctga gcgcttctta gaagtaccac ttctgacgaa agaagaagtc
    22861 ggccaggcaa tcgataaggt tattcggcag ttagaactca accttgacta tttcaaggaa
    22921 gatttcccta cgccagctac ctttgataat gtctatccaa tcatggataa cacggaatgg
    22981 accaatggtt tctggacagg agaactgtgg ttggcttatg aatacagtca acaggatgca
    23041 tttaaaaaca tcgctcataa aaatgttctt tctttcctgg atcgtgtcaa taagagagta
    23101 gaattggatc accatgatct cggcttcttg tacacaccgt cttgtatggc tgaatataag
    23161 ataaatggag atggagaggc tagagaagca accttgaaag ctgcagataa gttgattgaa
    23221 cgctatcaag aaaaaggtgg ttttattcaa gcttggggag acttgggcaa gaaagagcat
    23281 taccgtttga ttatcgactg cttgctcaat atccaactct tattctttgc ttatcaagaa
    23341 acaggcgatc aaaaatacta cgatattgca gaaagtcatt tctatgcttc agctaataat
    23401 gtaatccgtg atgacgcttc gtccttccac accttctatt ttgatcctga gacaggtcaa
    23461 ccctttaaag gtgtaacgag acaagggtat agtgatgatt catgctgggc acgtggtcaa
    23521 tcatggggag tctatggtat tcctttgact tatcgtcact taaaagacga gtcctgcttt
    23581 gacttgttta agggtgtgac caattatttc ttgaatcgtc tgccaaaaga tcatgtgtcc
    23641 tattgggatt tgatttttaa tgatggtagt gatcaatcac gagattcttc agcaacagct
    23701 atcgccgtct gtgggattca tgaaatgcta aaacatctcc cagaggtgga tgctgacaaa
    23761 gatatttata aacatgctat gcatgccatg cttcgttcct tgatcgaaca ttatgcaaat
    23821 gatcaattta cccctggtgg gacaagtctc ctccacggtg tgtactcatg gcattcaggt
    23881 aaaggagtgg atgaaggcaa tatctggggt gactactatt acctagaagc ccttatccgt
    23941 ttctacaaag actggaacct atattggtag gaggagaaat atgacaatgc caaatattat
    24001 tatgacccgt atcgatgaac ggttgattca tggacaagga caactttggg taaaatacct
    24061 aggttgtaat acggtcattg ttgccaatga cgaagtaagc acggacaaga tgcaacaaac
    24121 tctgatgaaa acagttgtgc cagactcagt tgccatgcgt ttcttccctt tgcaaaaggt
    24181 gattgatatc attcacaagg ctaatcctgc tcaaacgatc tttatcgttg taaaggatgt
    24241 gaaggacgct ttaaccttgg tagaaggtgg tgtccctatc aaagaaatca atattgggaa
    24301 cattcacaat gctactggta aagagcaagt gacacgctcc atcttcctgg gtgaagagga
    24361 caaggcagcc ctcaaggaat taagccaaac tcatcaagta acatttaata cgaaaacaac
    24421 tccaacagga aatgatggag ctgttcaagt caacattatg gactatattt aaaagaggag
    24481 atcgttatgt cgattaatgt atttcaagcg attttaattg gattatggac agctttctgt
    24541 tttagtggaa tgctattagg aatttacacc aatagatgta ttgttctgtc atttggtgtc
    24601 ggaattattc taggtgatct gcctactgct cttgcaatgg gagctattgg tgaattggct
    24661 tatatgggat tcggtgttgg tgctggaggt actgttccac caaacccaat cggacctggt
    24721 atctttggta ccttgatggc tatcactagt gctggtaaag tcagtccaga agcggctctt
    24781 gccctctcta ctccgattgc tgtggcgatt caattcttac aaactttcgc ctacactgta
    24841 cgtgctggtg cgcctgaaac ggcgatgaag cacttgaaaa accataattt gaagaaattc
    24901 aagttcactc taaatgcaac aatttggttg tttgccttta ttggatttac cttgggttgc
    24961 ttgggtgccc tttcaatgga taccttgttg aaactcgtag actacattcc accggtattg
    25021 cttacaggtt tgacagttgc tggtaaaatg ctcccagcta tcggttttgc gatgatcttg
    25081 tcagtgatgg ctaagaaaga gttgattccc tttgtcttgt tgggatatgt ttgtgcagct
    25141 tatctaaaca tcccaacaat tggtattgca attgtaggta ctatctttgc tttgattgaa
    25201 ttttataaca agccaaaaac agcggatcat gtggtagagg aggaagcaca cgatgactgg
    25261 atctaacaaa ttaacaaaac gtgattatct taaaacgtct ttgcgggcat tcttttgtca
    25321 aaatggattt aactatagta actatcaagg gttgggatat gccaatgtga tgtatcctgc
    25381 tttgaaaaaa cactatggag agaatcagga aggtttctac caagccttgg aagaaaactg
    25441 tgaattctat aataccaacc cacacttcct gccttttatt accagcttgc atcttgtaat
    25501 gttggaaaat ggccgtccgg caaaagaaac acgtagcatc aagatggcct tgatgggacc
    25561 attagcaggt attggggatt ctctctctca attctgttta gctccattgt tctcaaccat
    25621 cgcagcttcg tttgctcaag aaggcttggt tgtcggtcca atcttgttct tccttgcgat
    25681 gaatacgatt ttaacagcga ttaaattgtc aactggtctg tatggataca aactaggaac
    25741 gactgtgatt gataaactaa gcgagcagat ggcaacgatt tctcgtattg ccaatattat
    25801 tggtgtaacc gtaattgctg gtttggcagc gacatctgtt aagattatgg taccgatcac
    25861 ctttgctgca ggggaagtta aagcagacgc taaacaaagt atcgtaagta ttcagggaat
    25921 gcttgataag gttgctccag ctcttctacc agccctattt acacttttag tttactactt
    25981 gatcaaagaa aagaaatgga caacatataa actcgttatt ttaacagtta tcatcggaat
    26041 tatcggaagc tggcttaaga ttatagctta atggatacaa cattgtttta tggaatagtg
    26101 attgtcttgg cagtgagtcc ccttttactg tcaagctttc attctattcg tcagcaaaag
    26161 ttgcttcgca aacagatgga gcaacgacaa gagtatttag cttctttaac atctggtgat
    26221 gaagtgttgt tattgtctgg aattcatgga aaaatcattt ctatccaaga tgacttggtc
    26281 tccttgcaga ttgcaaaagg tgtggtcatc tatgtagaaa aggaaagtgt aatgggaaag
    26341 acaaaagaac tgctttttaa gtagttcttt ttctggtaat agataatgaa agaagaaaga
    26401 agacaatttt ttgaaagaat cgatggaaac caatgtcgtg attatatctt gtcccactgt
    26461 tcaaaagact atgagaaagt caagagttcc cttgaacgct tgatggacaa tcgttttatg
    26521 tttgatagtc cttgggatat ggagccttgt tcaaaaatcc atcaaatcca gccgatggta
    26581 tgggatcaag tatttgaaga tgatccagaa tggtcttata tgctcaatcg acaagaatat
    26641 ctcttgcagt ttatgatagg gtatgtggta gaaggagata aggactatat tcaaaagtgc
    26701 aagttctttc tatttgattg gattgagcag gtgagagaat tttctcctca atccttgatg
    26761 actagaacct tggataccgg tattcgttcc tttacttggt tgaaactact cttgctcctc
    26821 ttgaaatttg acttgctaga ggagaaagaa ctagagaaaa ttttggtcag tctagaaaag
    26881 cagattgact ttatgaaaag ccactatcgc gccaagtaca cccttagtaa ctggggaatt
    26941 ttacaaacaa ttccgatgct tgctatctat catttctttg cagataagat ggacctagaa
    27001 gaagcttacc attttgcttc agaggagttg aaacagcaaa ttgagacaca gattttagga
    27061 gatggaagcc agtttgaaca gtcgattctc tatcatgtag aggtttataa agccttgctg
    27121 gatttgtgtc tcttgcttcc agacttgcaa gatagttacc aagagttgct ggaaaagatg
    27181 gcgacctata ttcaaatgat gacaggctta gatggacgaa ctttggcttt tggtgatagc
    27241 gattctacag aaacgacaga aatgttgagc ctgtctgctg tggttttgaa caaggaagac
    27301 cttcttaacg gtctggatgt taaagtcgat ttgcttagcc tcttgttcct ggggcgagaa
    27361 aaggtcaagc gactgcagga atttgaaaag cgagcttggc agcctaagtt catgagcttt
    27421 gaagactctg gacatgtctg cattaaggat gaacatcgtt atctattttt caaaaatggt
    27481 ccgctaggaa gtgcccatag ccatagtgac gagaatagtt tttgcttaca gtatcaaggc
    27541 caacctattt tcatagatgc tgggcgttat tcttatcggg agatatatga acgttatctc
    27601 ttaaagagtg cttggagtca ttcgacctgc attgtagatg ggaaagctcc ggaaagaatc
    27661 acgggatcct gggaatatga atactatcct cactccctgt tttgtcacca taaagaaagg
    27721 gagggaatgc attatattga aggggcttat tggtcagcag aacctgattt gccttatctt
    27781 cacaggagaa aaatcctcat gttggtagag gatgtctggc tcttggtaga tgacatcagg
    27841 tgtcaaggtc agcatgaggc gttgactcag tttatccttg acaaggatgt gacctatcaa
    27901 gatgggaaaa tcaatcagtt gagactatgg agtgaagttg attttgattt ggaagatacc
    27961 atcatttctc ctaaatacaa tgagcttgaa agaagtagca aactcaccaa gcgccaattc
    28021 tttgagaatc agatgctgga ttataccatc attgcgcatg agagttttga aatcatccgt
    28081 cattctgtct accagacaga tgatcgtgaa gtggaaaatg ctctggcttt tgaagtgaaa
    28141 aatgacgaaa cagacaagct gattctgtta ttaagcgagg atatttgtgt aggtgaaaaa
    28201 ttgtgcctcg ttgacggaac aaaaatgcgt ggaaaatgtc tagtatatga taaaataaat
    28261 gagagaatga ttcgcttgca gtgctagaaa taggcatttt gaatagtgaa tatgttataa
    28321 taagtattag taggaggtgt tttagattgg agaagaaact gaccataaaa gacattgcgg
    28381 aaatggctca gacctcgaaa acaaccgtgt cattttacct aaacgggaaa tatgaaaaaa
    28441 tgtcccaaga gacacgtgaa aagattgaaa aagttattca tgaaacaaat tacaaaccga
    28501 gcattgttgc gcgtagctta aactccaaac gaacaaaatt aatcggtgtt ttgattggtg
    28561 atattaccaa cagtttctca aaccaaattg ttaagggaat tgaggacatc gccagccaga
    28621 atggctacca ggtaatgata ggaaatagta attacagcca agagagtgag gaccggtata
    28681 ttgaaagcat gcttctcttg ggagtagacg gctttattat tcagccgacc tctaatttcc
    28741 gaaaatattc tcgtatcatc gatgagaaaa agaagaaaat ggtctttttt gatagtcagc
    28801 tctatgaaca ccggactagc tgggttaaaa ccaataacta tgatgccgtt tatgacatga
    28861 cccagtcctg tatcgaaaaa ggttatgaac attttctctt gattacagcg gatacgagtc
    28921 gtttgagtac tcggattgag cgggcaagtg gttttgtgga tgctttaaca gatgctaata
    28981 tgcgtcacgc cagtctaacc attgaagata agtatacgaa tttggaacaa attaaggaat
    29041 ttttacaaaa agaaatcgat cccgatgaaa aaactctggt atttatccct aactgttggg
    29101 ccctacctct agtctttacc gttatcaaag agttgaatta taacttgcca caagttgggt
    29161 tgattggttt tgacaatacg gagtggactt gcttttcttc tccaagtgtt tcgacgctgg
    29221 ttcagccctc ctttgaggaa ggacaacagg ctacaaagat tttgattgat cagattgaag
    29281 gccgcaatca agaagaaagg caacaagtct tggattgtag tgtgaattgg aaagagtcga
    29341 ctttctaaaa tgaaggaaaa tgacttgcaa tctctgttaa gaaataaaat aatcccacct
    29401 agaacaagct aggtgggatt atttgcctat gaaatgagaa attatgggag caagctccta
    29461 aatcaactgt ttttgatcta cttctttaac tacttgataa aagttataga agtaggccaa
    29521 acttgaaatg atggttacga ctaggaatat tgaaaatttc cattggacag ggttggttaa
    29581 aagttgtgga aaggatatga ggagaaagaa gagggctgcg ttgaggacag gtatccgttt
    29641 tgattgtatt ttctcaagtc ctttattgag cgcaggaaga aagaggagta ggagtagtaa
    29701 aactgtatga gaaacagctc ctgaaataag ggcaaagaaa aggaaaatac tgataaaaac
    29761 atgaatgatc agtagtctag ctagtgattt cataaggtac ctcctaatcc tggtcttttt
    29821 tagctcttgc aatacgaagt gagtcgacaa tatgtatcat cactccgaaa aagaaagctc
    29881 ccagtatagt tttaaaaata tgttttgtat ttagaagaga actgataaaa tttggatttt
    29941 cacttgttag ggtatcaatg agtggaatta taaaaaatat cactgttcca taaatcgaac
    30001 ctgctttcag accaggataa cgtaactgtt tcttttcttt tttcatgagt ttgctccttt
    30061 tttaaaaatc ttcctcccaa aagagactgt tgaggtcagt ttggaggctg cgggcgagat
    30121 tgagacagag ttccaaggtt ggattgtact tgtcgttttc aatcatattg atagtctgtc
    30181 tcgagacacc gatatccttg gcgagttcga gctgggaaat acccaattcc ttgcgaaatt
    30241 ctttcacacg attcatctgg tctcctttct gatttatgtc gtatatattt gactatatta
    30301 tagtctttta aacataaagt gtcaagtatt tttgacatat tttttgaaga aatagtagtc
    30361 tccttgtcct atttgtctga caagtgcaag ctggtcggat ttgtggtaaa atagataaga
    30421 tatgacaaaa gaatttcatc atgtaacggt cttactccac gaaacgattg atatgcttga
    30481 cgtaaagcct gaaggtatct acgttgatgc gactttgggc ggagcaggac atagcgagta
    30541 tttattaagt aaattaagtg aaaaaggcca tctctatgcc tttgaccagg atcagaatgc
    30601 cattgacaat gcgcaaaaac gcttggcacc ttacattgag aagggaatgg tgacctttat
    30661 caaggataac ttccgtcatt tacaggcacg tttgcgcgaa gctggtgttc aggaaattga
    30721 tggaatttgt tatgacttgg gagtgtctag tcctcaattg gaccagcgtg agcgtggttt
    30781 ttcttataaa aaggatgcgc cactggacat gcggatgaat caggatgcta gtctgacagc
    30841 ctatgaagtg gttaatcatt atgactatca tgatttggtt cgtattttct tcaaatacgg
    30901 tgaggataaa ttctctaaac agattgcgcg taagattgag caagcgcgtg aagtgaagcc
    30961 gattgagaca acgactgagt tagcagagat tatcaagttg gtcaaacctg ccaaggaact
    31021 caagaagaag ggtcatcctg ctaagcagat tttccaggct attcgaattg aagtcaatga
    31081 tgaactggga gcggcagatg agtccatcca gcaggctatg gatatgttgg ctctggatgg
    31141 tagaatttca gtgattacct ttcattcctt agaagaccgc ttgaccaagc aattgttcaa
    31201 ggaagcttca acagttgaag ttccaaaagg cttgcctttc atcccagatg atctcaagcc
    31261 caagatggaa ttggtgtccc gtaagccaat cttgccaagt gcggaagagt tagaagccaa
    31321 taaccgctcg cactcagcca agttgcgcgt ggtcagaaaa attcacaagt aagagggaaa
    31381 aagatggcag aaaaaatgga aaaaacaggt caaatactac agatgcaact taaacggttt
    31441 tcgcgtgtgg aaaaagcttt ttacttttcc attgctgtaa ccactcttat tgtagccatt
    31501 agtattattt ttatgcagac caagctcttg caagtgcaga atgatttgac aaaaatcaat
    31561 gcgcagatag aggaaaagaa aaccgaattg gacgatgcca agcaagaggt caatgaacta
    31621 ttacgtgcag aacgtttgaa agaaattgcc aattcacacg atttgcaatt aaacaatgaa
    31681 aatattagaa tagcggagta agatatgaag tggacaaaaa gagtaatccg ttatgcgacc
    31741 aaaaatcgga aatcgccggc tgaaaacaga cgcagagttg gaaaaagtct gagtttatta
    31801 tctgtctttg tttttgccat ttttttagtc aattttgcgg tcattattgg gacaggcact
    31861 cgctttggaa cagatttagc gaaggaagct aagaaggttc atcaaaccac ccgtacagtt
    31921 cctgccaaac gtgggactat ttatgaccga aatggagtcc cgattgctga ggatgcaacc
    31981 tcttataatg tctatgcggt cattgatgag aactataagt cagcaacggg taagattctt
    32041 tacgtagaaa aaacacaatt taacaaggtt gcagaggtct ttcataagta tctggacatg
    32101 gaagaatcct atgtaagaga gcaactctcg caacctaatc tcaagcaagt ttcctttgga
    32161 gcaaagggaa atgggattac ctatgccaat atgatgtcta tcaaaaaaga attggaagct
    32221 gcagaggtca aggggattga ttttacaacc agtcccaatc gtagttaccc aaacggacaa
    32281 tttgcttcta gttttatcgg cctagctcag ctccatgaaa atgaagatgg aagcaagagc
    32341 ttgctgggaa cctctggaat ggagagttcc ttgaacagta ttcttgcagg gacagacggc
    32401 attattacct atgaaaagga tcgtctgggt aatattgtac ccggaacaga acaagtttcc
    32461 caacgaacga tggacggtaa ggatgtttat acaaccattt ccagccccct ccagtccttt
    32521 atggaaaccc agatggatgc ttttcaagag aaggtaaaag gaaagtacat gacagcgact
    32581 ttggtcagtg ctaaaacagg ggaaattctg gcaacaacgc aacgaccgac ctttgatgca
    32641 gatacaaaag aaggcattac agaggacttt gtttggcgtg atatccttta ccaaagtaac
    32701 tatgagccag gttccactat gaaagtgatg atgttggctg ctgctattga taataatacc
    32761 tttccaggag gagaagtctt taatagtagt gagttaaaaa ttgcagatgc cacgattcga
    32821 gattgggacg ttaatgaagg attgactggt ggcagaatga tgactttttc tcaaggtttt
    32881 gcacactcaa gtaacgttgg gatgaccctc cttgagcaaa agatgggaga tgctacctgg
    32941 cttgattatc ttaatcgttt taaatttggt gttccgaccc gtttcggttt gacggatgag
    33001 tatgctggtc agcttcctgc ggataatatt gtcaacattg cgcaaagctc atttggacaa
    33061 gggatttcag tgacccagac gcaaatgatt cgtgccttta cagctattgc taatgacggt
    33121 gtcatgctgg agcctaaatt tattagtgcc atttatgatc caaatgatca aactgctcgg
    33181 aaatctcaaa aagaaattgt gggaaatcct gtttctaaag atgcagctag tctaactcgg
    33241 actaacatgg ttttggtagg gacggatccg gtttatggaa ccatgtataa ccacagcaca
    33301 ggcaagccaa ctgtaactgt tcctgggcaa aatgtagccc tcaagtctgg tacggctcag
    33361 attgctgacg agaaaaatgg tggttatcta gtcggggtaa ccgactatat tttctcggct
    33421 gtatcgatga gtccggctga aaatcctgat tttatcttgt atgtgacggt ccaacaacct
    33481 gaacattatt caggtattca gttgggagaa tttgccaatc ctatcttgga gcgggcttca
    33541 gctatgaaag actctctcaa tcttcaaaca acagctaaag ctttggagca agtaagtcaa
    33601 caaagtcctt atcctatgcc tagtgtcaag gatatttcac ctggtgattt agcagaagaa
    33661 ttgcgtcgca atcttgtaca acccatcgtt gtgggaacag gaacgaagat taaaaacagt
    33721 tctgctgaag aagggaagaa tcttgccccg aaccagcaag tccttatctt atctgataaa
    33781 gtagaggaag ttccggatat gtatggttgg acaaaggaga ctgctgagac ccttgctaag
    33841 tggctcaata tagaacttga atttcaaggt tcgggctcta ctgtgcagaa gcaagatgtt
    33901 cgtgctaaca cagctatcaa ggacattaaa aaaattacat taactttagg agactaatat
    33961 gtttatttcc ctcagtgctg gaattgtgac atttttacta actttagtag gaattccggc
    34021 ctttatccaa ttttatagaa aggcgcaaat tacaggccag cagatgcatg aggatgtcaa
    34081 acagcatcag gcaaaagctg ggactcctac aatgggaggt ttggttttct tgattacttc
    34141 tgttttggtt gctttctttt tcgccctatt tagtagccaa ttcagcaata atgtgggaat
    34201 gattttgttc atcttggtct tgtatggctt ggtcggattt ttagatgact ttctcaaggt
    34261 ctttcgtaaa atcaatgagg ggcttaatcc taagcaaaaa ttagctcttc agcttctagg
    34321 tggagttatc ttctatcttt tctatgagcg cggtggcgat atgctttctg tctttggtta
    34381 tcaagtgcat ctagggattt tctatattgt cttcgctctt ttctggctag tcggtttttc
    34441 aaacgcagta aacttgacag acggtgttga cggtttagct agtatttccg ttgtgattag
    34501 tttgtctgcc tatggagtta ttgcctatgt gcaaggtcag atggatattc ttctagtgat
    34561 tcttgccatg attggtggtt tgctcggttt cttcatcttt aaccataagc ctgccaaggt
    34621 ctttatgggt gatgtgggaa gtttggccct aggtgggatg ctggcagcta tctctatggc
    34681 tctccaccaa gaatggactc tcttgattat cggaattgta tatgtttttg aaacaacttc
    34741 tgttatgatg caagtcagtt atttcaaact gacaggtggt aaacgtattt tccgtatgac
    34801 gcctgtacat caccattttg agcttggggg attgtctggt aaaggaaatc cttggagcga
    34861 gtggaaggtt gacttcttct tttggggagt gggacttcta gcaagtctcc tgaccctcgc
    34921 aattttatat ttgatgtaag aatggcaccc tgatgtttca gggtgttttt gtgtttaaat
    34981 acacaatgaa aatcaaagaa caaactagaa agctaacttt aggctgctca aaatatagta
    35041 gattgaaact agaatagtac acatctactt ct
//
LOCUS       DAVLFB010000005        34643 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLFB010000005 DAVLFB010000000
VERSION     DAVLFB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.005"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            188..1039
                     /locus_tag="VXD58_000244"
     CDS             188..1039
                     /locus_tag="VXD58_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0675004.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            complement(1271..1621)
                     /locus_tag="VXD58_000245"
     CDS             complement(1271..1621)
                     /locus_tag="VXD58_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675005.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(1761..2096)
                     /locus_tag="VXD58_000246"
     CDS             complement(1761..2096)
                     /locus_tag="VXD58_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675006.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(2184..3494)
                     /gene="der"
                     /locus_tag="VXD58_000247"
     CDS             complement(2184..3494)
                     /gene="der"
                     /locus_tag="VXD58_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV0675007.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(3508..4221)
                     /locus_tag="VXD58_000248"
     CDS             complement(3508..4221)
                     /locus_tag="VXD58_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV0675008.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(4218..5114)
                     /gene="dnaI"
                     /locus_tag="VXD58_000249"
     CDS             complement(4218..5114)
                     /gene="dnaI"
                     /locus_tag="VXD58_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV0675009.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(5115..6284)
                     /locus_tag="VXD58_000250"
     CDS             complement(5115..6284)
                     /locus_tag="VXD58_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV0675010.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(6285..6758)
                     /gene="nrdR"
                     /locus_tag="VXD58_000251"
     CDS             complement(6285..6758)
                     /gene="nrdR"
                     /locus_tag="VXD58_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV0675011.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            6898..7263
                     /locus_tag="VXD58_000252"
     CDS             6898..7263
                     /locus_tag="VXD58_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0675012.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            7268..7963
                     /locus_tag="VXD58_000253"
     CDS             7268..7963
                     /locus_tag="VXD58_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675013.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            7980..8747
                     /locus_tag="VXD58_000254"
     CDS             7980..8747
                     /locus_tag="VXD58_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675014.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(9100..10299)
                     /locus_tag="VXD58_000255"
     CDS             complement(9100..10299)
                     /locus_tag="VXD58_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675015.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(10296..11189)
                     /locus_tag="VXD58_000256"
     CDS             complement(10296..11189)
                     /locus_tag="VXD58_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675016.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11306..11386
                     /locus_tag="VXD58_000257"
                     /pseudo
     CDS             11306..11386
                     /locus_tag="VXD58_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11896..12084
                     /locus_tag="VXD58_000258"
                     /pseudo
     CDS             11896..12084
                     /locus_tag="VXD58_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12499..13116
                     /locus_tag="VXD58_000259"
     CDS             12499..13116
                     /locus_tag="VXD58_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV0675017.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(13436..14323)
                     /locus_tag="VXD58_000260"
     CDS             complement(13436..14323)
                     /locus_tag="VXD58_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0675018.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(14472..16355)
                     /locus_tag="VXD58_000261"
     CDS             complement(14472..16355)
                     /locus_tag="VXD58_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0675019.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            16732..18186
                     /locus_tag="VXD58_000262"
     CDS             16732..18186
                     /locus_tag="VXD58_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0675020.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRTSEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            18167..19132
                     /locus_tag="VXD58_000263"
     CDS             18167..19132
                     /locus_tag="VXD58_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0675021.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKPAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(19167..20441)
                     /locus_tag="VXD58_000264"
     CDS             complement(19167..20441)
                     /locus_tag="VXD58_000264"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV0675022.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(20441..21613)
                     /locus_tag="VXD58_000265"
     CDS             complement(20441..21613)
                     /locus_tag="VXD58_000265"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV0675023.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(21744..21992)
                     /locus_tag="VXD58_000266"
     CDS             complement(21744..21992)
                     /locus_tag="VXD58_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675024.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPISQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(22060..22685)
                     /locus_tag="VXD58_000267"
                     /pseudo
     CDS             complement(22060..22685)
                     /locus_tag="VXD58_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(22747..23196)
                     /locus_tag="VXD58_000268"
     CDS             complement(22747..23196)
                     /locus_tag="VXD58_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0675025.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(23198..23956)
                     /locus_tag="VXD58_000269"
     CDS             complement(23198..23956)
                     /locus_tag="VXD58_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV0675026.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(24071..26050)
                     /gene="pknB"
                     /locus_tag="VXD58_000270"
     CDS             complement(24071..26050)
                     /gene="pknB"
                     /locus_tag="VXD58_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV0675027.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(26047..26787)
                     /locus_tag="VXD58_000271"
     CDS             complement(26047..26787)
                     /locus_tag="VXD58_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV0675028.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(26802..28115)
                     /gene="rsmB"
                     /locus_tag="VXD58_000272"
     CDS             complement(26802..28115)
                     /gene="rsmB"
                     /locus_tag="VXD58_000272"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV0675029.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(28108..29043)
                     /locus_tag="VXD58_000273"
     CDS             complement(28108..29043)
                     /locus_tag="VXD58_000273"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV0675030.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(29056..31452)
                     /locus_tag="VXD58_000274"
     CDS             complement(29056..31452)
                     /locus_tag="VXD58_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV0675031.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHARDVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(31518..31832)
                     /locus_tag="VXD58_000275"
     CDS             complement(31518..31832)
                     /locus_tag="VXD58_000275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV0675032.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(31857..32483)
                     /gene="gmk"
                     /locus_tag="VXD58_000276"
     CDS             complement(31857..32483)
                     /gene="gmk"
                     /locus_tag="VXD58_000276"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV0675033.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(32613..34226)
                     /locus_tag="VXD58_000277"
     CDS             complement(32613..34226)
                     /locus_tag="VXD58_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV0675034.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(34296..34586)
                     /locus_tag="VXD58_000278"
     CDS             complement(34296..34586)
                     /locus_tag="VXD58_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0675035.1"
                     /translation="MEVEEKSIELSYEYVDDGYSGSNFERPNFKNMIDDLNKGKFSII
                     MVKDLSRFGRDYIESGKYLQKIFPEKGIRFISVNDNYDSDNADVSDIHLRFV"
BASE COUNT         9906 a         7818 c         6590 g        10329 t
ORIGIN      
        1 aaaaccatgt ttcaaaccag ataagttgaa tccaactcca aatcaaaaat ccagagtttt
       61 tcggtaaatt cttcaaatat tttttaaagc ccatgacttt ttccctcctt tttgatataa
      121 taataccacc tttttctacg ttttttataa atgtagaaaa tattcacatt tacctaggag
      181 gaacaatatg agatgggatt atggacaaat ttttaaagaa attcgaaagt caaaaggatt
      241 gacccaacaa gatgtatgtg gacaagtcat acatcgaaca actctaacaa atattgaaca
      301 cggtaaagtt attcccagtt ttgaaaacat ggtatttctt cttgaacaaa ttgatatgag
      361 cttggcagaa ttcaagtata tatgcaacga ataccatcct agtaaaaggc gagatattat
      421 tgtagagagc caaaatccgt ctacttttca agatactaga aaaatggttg aactcactga
      481 gaaatgtcaa aaatatctta agacacatca cgatgttcct attcaaaata tctatcgtca
      541 tacaaaaatt gtcacagagt tacgaactaa aggattcaaa aacaaccacg tcttgaaaga
      601 tttgtatgaa gaaatttggg actatcttga acctatggat acatggtaca ttagtgattt
      661 gaaattgctt ggaaccattc tctttttctt tccttctgaa aatcttcccc ttcttattga
      721 tagaattatg aaaaccatcg agaaatataa atacttccga gaaacaaaag catttttatc
      781 atctttttta gccaatctct ccactgtata ttttcaacat catttattca aagaatgtga
      841 aacaatcacc ctacaacttt tggtattagc agaagaatta aaaatatacg atattcttgg
      901 attctctcaa gtacgcttgg gaatactaca acataactcc gatttgattg ataaaggaat
      961 aactttatta cgactaacca aagaagaggc attagttaaa atactcgaaa aagaaattaa
     1021 tgatttttca aacctataat ttgtaataac tctcatcatt ttattatagt agattgtaat
     1081 agaatgtaat agaatgtgaa cgaatcaatt aggggatcta aagcatttta taccaatgtt
     1141 ttataagttg tattgtatta ttccatattc aatccactat acaagatgaa tatttctctc
     1201 aagtttcata atcaaaataa aaatgactgt atctatagct ccataaacaa atcatagata
     1261 cagtcatttt ttagttccta caccttcctt tatgcacacc tttagatttc cctagttccc
     1321 gcctcaagcg aatcattttt ttgaaaaaga cataggctaa ccaatcacta gcaaaagcga
     1381 taagtagtaa taatagccaa ataccccata acttgatatc tgtcaaattt ctcaagacga
     1441 tattgaaaaa cagaactgaa aaaactgtcc aagcaaggct aaaaagagca tagagggtga
     1501 tgtaaatcca gtaaaaatag taaaaaattg ggaaaaattt actttctcta ttatccttgt
     1561 caatccagct gaaaaagtaa aaacagggta ataagattat cattttaaac aagtgttcca
     1621 tcacaagcct ccttttttaa attgttttct ttagtttcgt tctattaaat gttattacca
     1681 atataaacct tggaatagcg tgtaataaat gattttagag gtaccataag ccacctccta
     1741 caaatagaaa ccgatataaa tcaatgcctt ccacccttag acttccctag ttcctgtctc
     1801 aagcgaaaca tttctttgaa acaggaataa gttaaccaat tcataccaat agctagcaga
     1861 ataaaaagaa accaaatgcc ccataacttg atatctgtca catttctcaa gacggtattg
     1921 aaaaacagaa ctgaaacaac tgtccaagca aggctaaaaa gagaatagaa ggggatgtaa
     1981 aaccagtaaa aataataaaa aattggaaaa aacttactat ttctgttggc cttttcaatc
     2041 cagttatcaa aataaaagta cggtgctaaa agtaagaatt taaacaaatg ttccatcacc
     2101 gacatccccc ttcttttgat agcgttttct attattttat tatatcaaaa aaatccggaa
     2161 ctgtcattcc agattctact tttttatttg cgttttcttg cgatgagatg aatcggtgtt
     2221 ccctcaaaaa caaaggcctt gcggatttga ttttccaaga aacgcaggta agaaaagtgc
     2281 atgagttctt cttcattgac aaagatgaca aaggttggtg gtttggttgc cacttgggtc
     2341 gcatagaaaa tcttgagacg ttttcctttg tctgtcggtg ttgggttgat ggcaatggca
     2401 tccatgatga catcgttcaa gacagctgat ggaatacgtg tattttgact ttcgctgatt
     2461 tgcttaatca tctcaggaag tttgtggaga cgttgcttgg ttaaagctga tacaaagata
     2521 atcggtgcgt aaggcaggta ttggaactgc tcacggatat cttcttccca gtttttcata
     2581 gtgtggttat ctttttcaag cgtatcccac ttgttgacca cgataatcat ccctttacca
     2641 gcttcatgag caaatccagc gatacgcttg tcgtattcac gaatcccctc ttccgcattg
     2701 atgaccatta agaccacatc tgaacggtca atagcacgca tggcacgcat gacagagtac
     2761 ttctcagtat tttcataaac cttaccagac ttacgcatac cagccgtatc aatcatggta
     2821 aactcttgac catctgtatc tgtaaagtgg gtatcaatag cgtcacgagt tgttccagca
     2881 acaggactgg caatgacacg gtcttctccc aagatagcat taatcaagct tgattttcca
     2941 acgttaggac gaccaatcaa gctaaactta atgacatctg gattttcttc ttcatattca
     3001 tttggaaggt tttccacaat cgcatctagt acatcccctg taccgatacc atggacagat
     3061 gagataggca gtggttcacc caaaccgaga gcatagaaat catagatatc atttctcatc
     3121 tcagggttgt ccaccttgtt gactgcgagg ataactggtt tgtgggtctt ataaagctta
     3181 cgagctacgt attcgtctgc atcagtaatt ccttccttac cagacacgac aaaaacgata
     3241 acatctgctt cttccatggc aatttctgcc tggtgcttga tttgttccat gaaaggagca
     3301 tcgacatcat caattcctcc tgtatcaatc atgctaaaag aacgattgag ccactcaccc
     3361 gttgcataaa tacggtcacg tgtcactcct tcgacatctt ctacaatgga gattcgctca
     3421 ccagcgatcc gattaaatag ggttgatttc ccaacattgg gacgtcctac aatggcaata
     3481 gttggtaggg ccataatttc tcactttcta caataatttc ttctgttcaa gattttttct
     3541 agttgagctt ggttcagctt gaccaaactg ttctgctagg cgctgactcc agcttgtggt
     3601 cgcacgcgcc ccagcatagt cagcctgaac acggtcataa gcttggattg cctcagttga
     3661 ctgttcttgg tattcttcct caaagacaac attctctagt ggcagtctcg gtttcatatc
     3721 atgatgttga tttggcacac ccagtgccat cccaaagaca gaataggtgt agtcaggtag
     3781 gttaaagagc tctgccactt cttcagactt gtatcgaacc aaaccgataa tcacaccacc
     3841 atagcccaag ctttcagctg ccaacaaggc gttttgtcca gcaagagctg catcgaccga
     3901 actaatcaag agaccttcca caccttgggg ttggaaggtg tcggtatgaa gtctggctcc
     3961 cttttctgct cggttcaaat ctccgacaaa gagaaggaaa acagcagact ggcgaatggc
     4021 ttcttgaggt accaattcat acaaggcatc tttcttctct tgacttcgta ccacaatcac
     4081 agagtaggat tggaaattct tccaagatga tgccatctgg gctgctgtca aaatctcatt
     4141 taagtctact tggggaattt cttgctcttt aaacctgcgc actgaagtat gagccttcat
     4201 caatttaatg gtttctgtca tcgacggttt actccttcta aacgagtctc ctcagccaaa
     4261 taacggatgc gttccatgac ccgtctggct tcccaggttt cgtcatttcc atgtttcact
     4321 ttcgcaaaat gcttctccaa atcttcaaag ttgaagttgg atgtgaaaaa ggtcggtaaa
     4381 ttttcctgca tccgatattg gagaatgacc tgcaggattt catcacgcac ccaagcggtg
     4441 gattgctcgg cgccaatatc atctaaaatc aggacctcag acagcttaat ctcatccacc
     4501 aaggtcttaa cattgccatc actgatagca tttttgacat caatgacaaa gctaggatag
     4561 tggaggagag ttgatgaaac accacgtttt tctgataaat catgagctaa ggccgccacc
     4621 atgaaacttt tacccacacc aaagtctcca tataagtaaa gaccttttcg aatagctgga
     4681 tattgctcca cgaaggctag tagcttttca aaaactggta agcgccccaa atcatccaag
     4741 tcaacttgag ccaaactagc tttcttgaga ctggctggta gattgattaa cttgagacgg
     4801 ttcttaatag ccgcttcttt ttcagccgcg attagctcag gagtttcttc atatgaaaca
     4861 tctgcatagc catgattctt aaccaaaatc ggcttgtagc ctttggcaat ataatccgta
     4921 tccccacgga gaaacttgtc acgctcggtg atgtactgat taaacttgga gatactgcga
     4981 tttaattcct ctggagtcaa ggattcttgc tggataaaag ccgcaacatc agggtccttc
     5041 atgattttct ggaccaaatc ttgataataa aaacggctgg gttgacgttt gagtacgtct
     5101 ccgacacttt ccatctaatc tcctcctttt tctaatcgag ctaatagttc ttgcttctta
     5161 cgttctagtt ccagacgagt ttcctcgctg gtttcattct tatattcagg attactccat
     5221 ttaggaacat tggtttttac tggggcagtc tgattctgtt tttgtgtttt tgctttctgt
     5281 cctcgctcac ggatgcgcaa gactgccttt tctgccgaat gaatcttttg ataggcatag
     5341 tcattggcta ccttcatggc atatttctca ttgatatttg ccgaatcgac cttattaaag
     5401 gtcaataaaa gaatgatatt gatgacttcg tccagcaagc ccaagccagc catctgttgc
     5461 aagagttctc tttctgtttg ggtaatggtt cccttgcgtg tttgcttgat ttctgccaag
     5521 aactgcaggg cagttttact tttggcttct ttgataatgg ttgtttcctt aagactaaag
     5581 tcagaggaaa ctggtttttg agcaattttt tcacgcatgc gtttggttga aataacctga
     5641 gaaacagctg ttgacttggc caattgatag gtttcaaacc aagtccattt cttctcctcg
     5701 gcaatagcaa agaggtttaa gacatcggac tgctcatccg caaaacgaag tccatctcga
     5761 gccatcagct ggcgaaaatg ttccaagtca aaatcattgg ccactttctt cttgagacca
     5821 aggtcttctt gactgcctag ttctgccaat tctggaaaga cttgattgag tgagacaggt
     5881 atttcttcac catcagcact ttcaactttc aaatcctcca cagcagagtc gccaatcttt
     5941 ttctctaaga gtctgcgata aacaggatgc tccaagaagt cttgactaga tagaggagca
     6001 tggagggcta gctgataaac atcccccttt tgatagaggg tcaagagatt aaaagcagat
     6061 aagattttca atgattttat cagtctatcc atcccaaagt tgagatgatt gaggatactt
     6121 gaaaaaagat attcctttct accattatcc caaaaactga ttgtataaag ataaaggctc
     6181 agtgcctcct gaccgataat cgggaggtag cactgtacca gagatgaggt atcttgcgac
     6241 acccgattat tctttagata agaaaaacgg tcaattggct tcatttatct ttcctttttc
     6301 tttttagagg actgggtgat ttgttggagc aagctctcta actcactgac atccttaaaa
     6361 ctacgataga cactagcaaa acgtacatag gtaatctcgt ccaattcagc caactcctcc
     6421 atgacgagtg aaccaatgtc ctcactttga atttcatttt catttcgacc acggagtttc
     6481 tgttcgatac gattgactac catgttgatt tcatcacttg acacaggacg tttctgggct
     6541 gagcggataa tcccattaaa gattttatct ctggagaatt gttcccgtgt gccatctttt
     6601 ttaacaacca ctaaggttct ttcttctact cgttcgtagg ttgtaaaacg gtgttggcac
     6661 tcgtcgcact ctcgtcttct acgaatggtg ttcccttctt ctgcttggcg actatcgata
     6721 acacttgact tggtagcccc acattttgga caacgcatcc tttccctcct tatcgttttc
     6781 ttttcattat accatttttt aagcgattcc caaaacaatt cttctttttg cttgacaagt
     6841 tttttgtttt gttgtattat ttaattaaga caaaaggata agagaaagga gactaagatg
     6901 tcctggacat ttgacaacaa aaaacccatc tatttacaga ttatggagaa aatcaagctt
     6961 cagattgttt cccatacact ggaacccaat caacaacttc caaccgtgag ggagctagct
     7021 agcgaggctg gtgtcaatcc caataccatc caaagagcct tatcagacct tgaacgagaa
     7081 ggatttgtct acagcaagcg aacaactgga cgatttgtga ctaaggataa ggagctaatc
     7141 gcccagtcac gcaaacaatt atcagaagaa gaattggaac acttcgtttc ctccatgacc
     7201 cattttggct atgaaaaaga agaactacca ggcgtagtca gtgattatat taaaggagtt
     7261 taagcctatg tcattactag tatttgaaaa tgtatccaaa tcatatggag caacaccagc
     7321 ccttgaaaat gtttctcttg acattccagc tggaaaaatt gtcggccttc ttgggccaaa
     7381 cggctcagga aaaacaaccc tgattaaact aattaatggc ctcttacaac cagatcaagg
     7441 acgtgtcctc atcaacgaca tggacccaag cccagcaacc aaggccgttg tagcttattt
     7501 gcctgatacg acctatctca atgagcaaat gaaggtcaaa gaagccctaa cctacttcaa
     7561 gaccttctat aaagatttca atcttgaacg cgcccatcat ctacttgcag acctgggcat
     7621 tgatgaaaat agtcgtctca agaaactatc aaaaggaaac aaagaaaagg ttcaactgat
     7681 tttggttatg agccgtgatg ctcgtctcta tgttttggac gaacccattg gtggggtgga
     7741 tccagcagcc cgtgcttata tcctcaatac cattatcaac aactactcac caacttctac
     7801 cgttttgatt tctacccact tgatttctga tatcgagcca atcttggatg aaattgtctt
     7861 cctaaaagac ggaaaagtcg tccgtcaagg aaatgtagat gatattcgct acgagtcagg
     7921 tgaatccatt gaccaactct tccgtcagga atttaaggcc taagcaaagg agattattta
     7981 tgttttggaa tttagttcgc tacgaattta aaaatgttaa caagtggtat ttagccctct
     8041 acgcagccgt gctagtcctt tctgccctca tcggaataca gacacaaggc tttaaaaatc
     8101 taccttacca agaaagtcag gctactatgc tactttttct agctacagtc tttggtggct
     8161 tgatgcttac acttgggatt tcaaccattt tcttgattat taaacgcttc aaaggtagtg
     8221 tctacgaccg acaaggctat ctgactttga ccttgccagt ttctgaacac catatcatca
     8281 cagccaaact aatcggtgcc tttatctggt cattgattag caccgctgta ttggctctaa
     8341 gtgctgttat tattctggct ttaacagctc cagaatggat tcctctttct tatgtgatta
     8401 catttgtaga aacacatctc cctcagatct ttcttacagg tatatccttc ctactaaata
     8461 ctatttcagg aatcctctgc atctacctgg ctatttccat tggacagctt ttcaatgaat
     8521 accgtacagc actcgctgtt gcagtctaca ttggtatcca aatcgtcatt ggatttattg
     8581 aacttttctt caatcttagt tctaatttct atgtcaattc actggtagga ctcaatgacc
     8641 atttctatat gggagcaggt atagccattg ttgaagaact catattcata gctatctttt
     8701 atctcggaac ctactacatc ttgagaaata aggttaattt gctttaaata atttttacct
     8761 agatatgtaa catactcata gaacaaaaga gaccaggcaa aaagtcttta caattagaaa
     8821 acgcatagta tcaagtgtta aaaatccttg atattatgcg ttttttgtga gctgacttat
     8881 ttcctttcac tatatcgcaa aatgaaataa gaacggaacg atgggatttt ggaattcaaa
     8941 tcaatttata agaatgtttt agaagtaata ttatcctatt ccagattcag ttcactatac
     9001 aattgagttt tcaagcaacc tgtttacata atgtgtacat aattaggttc gtgattccac
     9061 ccttttcacc tttaaaaacc tcgctttcgc aaggctcttc tatttataag ataaggcacg
     9121 tttaaaggtt ttccaaatcc ctaaatcatc cgtttgaaga acgagactag catacatgcg
     9181 tccgataaat cctgttgcta ccaccgcaaa aatcactgta atagcaagtg aaatccatgc
     9241 ttccgctccc tccgcatagc cattaatcgt tcgaaacggc ataaagaagg tcgaaataaa
     9301 gggaatataa gaaccaatct tcaagaggag attgtcacca gctgcaccta gagctgtcac
     9361 tccaaaaaaa ccacccataa tcaaaatcat caaaggcgac aaggctttcc ctgagtcttc
     9421 aggacgagaa accatagatc ctaggaaggc tgccaagact acgtacatga aaagactgat
     9481 caaaataaag agcaaggtat tcagtgagat agcatctccc aagtgatcca aaataccaga
     9541 ctgagccaag aatggcaaat ctttaaagag caaaacggca gccagaccac ctacaacata
     9601 gatcccaata tgcgttaaaa tcactagaaa cagagccatc atccgcgcat agaaatagtg
     9661 acttgccctt atgctagaaa agacgacttc cataattttg gtgccttttt cactggcaac
     9721 ttcctgagct gttacacccg cataggtaat cagaatcata taaagaaaga atcctaaggc
     9781 acctgctgca attgtttgaa taaacttttt attttccttg gcttcatcaa tcttttctgt
     9841 gaattgaatt gtctgcgcta agcgtttttc ctgctcttga gacaaggaag cagttgaacg
     9901 attaagctga ttttgcagtt cattgagtgt acctgtaacc tcaaatttaa ttccattttc
     9961 aagcgatgtt tcgccatgat aaactgcctt tagaacacta tcttcttgat caatggtcaa
    10021 ataacctttt aatttttctt ctttaattgc ttctttggca cttgcttcgt ctttatagtc
    10081 gaagttaaca ccatttacat tcttcagtcc ttctgctaca gatggcactg ttgtcactac
    10141 tgccacttta ttatttttag ccatagaaga accttggaga tgcccaattc ctacagagat
    10201 tcctaaaaag aggaacggcg aaatcaccat aaagaagaaa ctccatgact cgacatgtcg
    10261 aagataggtt tccttgatta caacccacat atttctcata cttccactcc tgattctagt
    10321 ttaaagattt catcgatagt tggcgcttgt tggtcaaatg ttgcgatata ttgaccttga
    10381 gtcaagattg ggaagagttc ccttccagcg ctctcatcct ccaaaatcaa tttccaactg
    10441 ccttgtttgg tcaagctcac ctgtttgaca tgaggaagat tttccaattc ttccttgctt
    10501 cgttcacttg aaacaaagag acgcgttttc ccgtattgat tgcggacatc ctgaactggt
    10561 ccgtgcaaga ccacacggcc atctcgaatc atcagaatat cgtcacaaag ttcctcaaca
    10621 ttggtcatga catggtcaga aaagataatg gttgctccgc gctctttttc ctgaaaaatg
    10681 acttgtttga gcaattctgt attaactggg tccaatccac taaaaggctc atccaagata
    10741 atcaggtctg gttcatgaat cagagtaata atgagctgaa tcttctgctg atttcctttt
    10801 gacagactct tgattttatc tgtcagcttt ccttttactt ccaacctctt catccattga
    10861 gggagttttt ctttgacttc tttggcatcc atgcctttta gagtcgccaa gtagcgaact
    10921 tgttcaagaa ctgtcaattt aggcatgaga ctgcgttctt caggcagata accaatccga
    10981 gcatagatct cctgacgaat atcctgacca tccagaccga tttctccctg atattctagg
    11041 aattttaaaa tactatggaa aatcgttgtt tttccagcac cattttttcc gactagtccc
    11101 aaaatacgac ctggtcgcgc ttgaaagtca ataccaaaca aaacttgctt ggatccaaaa
    11161 cttttctcta gacttcttac ttctagcatc tttcacctcc gaaatttctt gcactcatta
    11221 tactcctttt tgatagcctt tacaatgttt tttgtccatt tttagaagac tattgctgtg
    11281 taaaatatgg cctggagcac ttttatactc aatgaaaatc aaagagcaaa ctaggaagct
    11341 agccgtagac tgctcaaagt acagctttga ggttgcagat aaaactgacg aagtcagctc
    11401 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaactata cgtacggcaa
    11461 ggcgaagctg acgtggtttg aagagatttt cgaagagtat tagtgataaa tccattatac
    11521 agcagcaaac ttaatttata ccttccgctc ctcaactgtc tatttttaat cctgaattgt
    11581 tatttgagta actccttttt cctcgtaaag ttttcttcct ctaaaacttc tggaaaaagg
    11641 ctaatagttt cagacaacat ttttataaga aacaagttca tctgtcattt caagaaggag
    11701 taatccttta tctactaatg gacggaacag aattcaaccg cttgtccgat atgttttcta
    11761 aggattatat agtaaaatga aataagaaca ggacaaattg atcaggacag tcaaattgat
    11821 ttctaacaat gttttagaaa tagatgtata ctattctagt ttcaatctgc tatatctatt
    11881 atgcacaccc ctataggatc taatgaaaat cacaacaggc tcattcatag atagttacct
    11941 caggaaacta agaaaacgac tacaaaggaa gtcgcattca tcgaaaagtg gattaacaac
    12001 tatcctaaaa aatgcttgaa ctacaagtcc cccagagaag acttctggat gactaacttg
    12061 aacttgaaat ttagcaataa ttaattcacc atctaactat atttagtaat tatttcagaa
    12121 ctgattaata ttaaaattaa ctaacaattc aaaggattca tactagccat aaattacgtc
    12181 catcagagag agactcttac tacttttaga ttttagtctt tctagcttca gaatacatct
    12241 aaactttagg gaaaatgact attcgaaagc gcgaatgcct caaaattatc tcagataagc
    12301 tattcgaaac ttagaatgct tttaaattta tggaattgcg attattcgaa acctagaatg
    12361 catataacct ttagttgaca gacctattct aagtctcgaa gggctattta ctttctattc
    12421 cttatcaaaa aagactcatt ccccctttct cctccaaaat atggtatagt agaaatatac
    12481 tatctatgag gagtttacat gtcacaggat aaacaaatga aagctgtttc tccccttttg
    12541 cagcgagtta tcaatatctc atcgattgtc ggtggggttg ggagtttgat tttctgtatt
    12601 tgggcttatc aggctgggat tttacaatcc aaggaaaccc tctctgcctt tatccagcag
    12661 gcaggcatct ggggtccacc tctctttatc tttttacaga ttttacagac tgtcgtccct
    12721 atcattccag gggccttgac ctcggtggct ggggtcttta tctacgggca catcatcggg
    12781 actatctaca actatatcgg catcgtgatt ggctgtgcca ttatctttta tctagtgcgc
    12841 ctatacggag ctgcctttgt ccagtctgtc gtcagcaagc gcacctacga caagtacatc
    12901 ggctggctag ataagggcaa tcgttttgac cgcttcttta tttttatgat gatttggccc
    12961 attagcccag ctgactttct ctgtatgctg gctgccctga ccaagatgag tttcaagcgc
    13021 tacatgacca tcatcattct gaccaaaccc tttaccctcg tggtttatac ctacggtctg
    13081 acctatatta ttgacttttt ctggcaaatg ctttgacacg taaaaaatcc gtttggtttc
    13141 ccaagtggat ttttaaagcg tagattaact atagcttgat actaaatata ctttggtatg
    13201 gaaatcatgc atatttttcg atagtgaagc gaggacttac ctagcctttc caccgtgata
    13261 gaaacacctg aaatctaatg gtttcaggtg ttcggaaact ttgagcctag tgtctcaaag
    13321 tttaggtatg gaatttcgaa gaaagtcgct accgtccgta atcacttaag gaaaggctca
    13381 aaaatattgt tttcaaccac aaaatccgtt tggtttccca agcggatttt gtgctttatt
    13441 ttgaaacttc ttttgcaaga acaaagttcc caagtgtggc agaaccattt cctgcgactg
    13501 ctggcgtcac gatatagtca cgcacatctg gtactggtag gtaaccatta agaagagatg
    13561 taaatttctc acggacacgg tccagcatat gttgttgagc catgacccct ccaccaaaga
    13621 caatcacgtc tgggcggaaa gtcactgtcg cattaaccgc agcttgagcg atatagtagg
    13681 cttgaacatc ccaaacaggg ttgttgagtt caatagtttc cccacgtaca cctgtacgag
    13741 cttccaaact tggaccagct gcatagcctt ccagacatcc cttatggaaa ggacaaacac
    13801 ccttaaactc tttttcaata tccattgggt gtctagcaac ataataatga cccatttcag
    13861 ggtgacccac accaccgata aactcaccac gttggatgac gcctgcaccg atacctgtac
    13921 cgattgtgta gtaaaccaag ttttcgatac gaccaccagc attgttacgg gcaaccactt
    13981 caccgtaagc agagctgttt acgtctgttg tgaagtacat tggcacgttt agggcgcgac
    14041 gaagggcacc aagcaagtcc acatttgccc agtttggttt tggagtcgtc gtgataaagc
    14101 cataagtttt tgagtttttg tcaatatcaa tcggcccaaa tgaaccaact gcaagaccag
    14161 caaggttatc gaattttgag aagaactcaa tggttttatc gattgtttcg attggagttg
    14221 ttgttggaaa ttgtgttttt tctacaacgt taaagttttc atcaccgaca gcacagacaa
    14281 actttgtacc gccagcttcc aagcttccat ataattttgt catgataaac ctcttgtttt
    14341 tattttcttt attatagcat acttcgaaag tctaaatgtc tctatttttt agattttcct
    14401 ctgtaaatct tactatctaa taaaaacgaa caaacatgtc atttgttcgt tttcacatta
    14461 gagaggattg attagatttt cacttcgatc acagcatccc ccttcgcaac tgaacctgtt
    14521 gcgactggag ctactgaagc gtagtcagct gtatttgtaa cgataaccat tgttgtatca
    14581 tcaagtccag ctgtagcgat tttatttgag tcaaatgttc caagaacatc gccaactttc
    14641 accttatcac cttgagcaac ttttgcttca aaaccgtcac cgttcataga tacagtgtca
    14701 ataccaacat gaatcaaaac ttcagcacca tttcttgttt tcaaaccaaa agcgtgccct
    14761 gttggaaagg caattgaaac ttcagcatca gctggtgcat agaccacgcc ttggcttggt
    14821 ttcacagcga taccttgtcc catagctcca cttgagaaga ctgggtcatt gacatcagca
    14881 agagcgacaa catcaccgac gataggagtt acaagtgttt cattttgaag agctgctggc
    14941 gcaacttctt ctttttcttc agccacttca gcttgttttg cagctgcagt tgcgtctact
    15001 tcatcttcgt aaccaaacat gtaagtaaga gcaaaaccaa gggcaaatga tacagctacc
    15061 ataagaaggt attgtggaag ttgtccgtta ccaacataaa gcattgtacc agggatgatg
    15121 gtgataccat taccagtacc agcaagtcca aggatagaag ccaatccacc accgattgca
    15181 ccagcaatca atgaaaggaa gaatggttta cggaagcgca agttcacccc gaagatagca
    15241 ggctctgtaa tacctaggaa ggcagaaaga gcagccggga aagcaagtgt tttcagtttt
    15301 ggattttttg ttttaacacc aaccgcaaca gtagcagcac cttgagctgt catagcagct
    15361 gtgatgatag cgttgaatgg gttagcatgg tcagcagcaa gtaattgcac ttcaagcaag
    15421 ttgaagatgt ggtgcacacc tgacacgacg atcaattggt gaaccccacc aatcaagaaa
    15481 ccaccaagac caaatggcat gctaagaatc gcttttgtag caataaggat gtagttttca
    15541 acaacgtgga aaactggtcc aatgacaaag agtccaagga tagacatgac caaaagtgtc
    15601 acgaatggtg ttaccaagag gtcaatgaca tctggaacaa ccttgcggac agctttttca
    15661 aatttagctc cgacaacccc gatgatgaag gctggaagaa cggaaccttg caaaccaaca
    15721 acagggatga aaccaaagaa gttcatcgct gttacttcac caccttgagc aactgcccaa
    15781 gcgtttggaa gtgagccaga gacaagcatc ataccaagaa cgataccaac ggcaggattt
    15841 ccaccaaata cacggaaggt tgaccacaca accaaacctg gcaagatgat gaaggctgta
    15901 tctgtcaaga tttgtgtgta agttgcaaag tcacctggaa gtggcatttc aagagcgttg
    15961 aaaagaccac gcacacccat gaagagacct gtcgctacga taactgggat gattggaacg
    16021 aaaacatcac caaaagtacg gatagcacgt tggaaccagt tcccttgttt agcaacttct
    16081 gctttcatgt catccttaga tgatgttggt aatccaagta caacaacttc atcgtacatt
    16141 ttgttaactg tacctgtacc aaagataatt tggtattgcc ctgagttaaa gaaagcacct
    16201 tgaacttttt ccaagttctc aatcacttct ttattgattt tctcttcatc tttgaccatg
    16261 acacgtagac gtgtcgcaca gtgggcaaca ctattgacat tttcacgtcc gcccaaggca
    16321 tcgatgactt tttttgcaat ttcctgattg ttcatttgca aaaatctcct tatataacat
    16381 tttgttcttg tttgaaagcg attttattcg cccttacgac tattatttta tcatgtttta
    16441 aaaatatgtc aagcgttttg cagaaaaaat attctatcta cttagtgggc aagctttaga
    16501 ttgattgttt ttgactgttt catcaggaat ttcttgaaaa ataagtttga aaccttggtt
    16561 aaaaagtctt ttatttcatt atctttcaca ttttcgtaaa aatttgattg atttcctgta
    16621 tttattttat gataatcgtt tgacagtttt ttctcttttt taacataaag ggcttgcatt
    16681 ttttatcgaa aacagttact attaaaagtg ataagatttt tggaggaatg aatggaatgg
    16741 acaactgagc gtcgttacag actttatcaa gattggacgc aagaagaaat tcaacatata
    16801 aaggaaaata tggcacaatc tccatggcat actcattacc atgttgagcc aaaaacagga
    16861 cttctcaacg acccaaatgg cttttcttac tttgatggca agtggatcct cttttaccag
    16921 aattttcctt ttggtgcagc ccacggttta aaatcttggg cacagctaga aagtgatgat
    16981 ttggttcact ttagagaaac tggagtcaaa gttttgccag atactccatt agatagtcac
    17041 ggtgcctact ctggttctgc catgcaattt ggcgataact tattcctatt ttatacagga
    17101 aatgttcgcg atgaaaactg gatccgtcac ccataccaga tcggtgcttt gatggacaag
    17161 gagggtaaga ttacaaagat tgacaagatc ttgattgacc agccagcaga ctctactgac
    17221 cacttccgcg atccacaaat ttttaacttt aaaggtcaat actatgctgt cgtcggtgga
    17281 caggacttgg agaaaaaagg cttcgtccgt ctctacaagg ctgtcaataa cgactacaca
    17341 aactggcaag cagttggcga ccttgacttt gctaacgacc gtactgccta catgatggaa
    17401 tgtcctaatc tggtctttgt agaggaacag cctgtccttc tctactgtcc acaaggattg
    17461 gataagaaag ttctagacta cgataatatc tttccaaata tgtataagat tggggcttcc
    17521 tttgacccta aaaatgccaa aatggtagat gtgtctcaac ttcaaaacat ggattacggt
    17581 ttcgaagcct atgcaactca agccttcaac gctcctgatg ggcgtgctct agcagttagc
    17641 tggcttggtt tgccagatgt ttcttaccca tctgaccgtt ttgaccacca aggaaccttc
    17701 tctttggtca aggaactcac tatcaaagac gacaagctct accagtatcc agtcgctgct
    17761 attaaggacc ttcgtacttc tgaagaagcc ttctcaaacc gttcccaaac caagaacact
    17821 tacgaacttg aactcaactt ggaagctaat agccagagcg agattgtctt acttgctgat
    17881 aaagaaggta agggactttc aatcaacttt gaccttgtaa acggtcaagt gacagtggat
    17941 cgtagccagg ctggagagca gtatgcccaa gaatttggga taactcgttc ttgccctatc
    18001 gagaatcagg ctactactgc tacaatcttc atcgataact ctgtctttga aattttcatc
    18061 aataaaggag aaaaagtatt ttctggtcgt gtcttcccac atgcggacca aaatggtatc
    18121 ctgatcaaat ctggaaaccc aactggaact tactatgaat tagattatgg tcgcaaaact
    18181 aactgatgtc gccaaacttg caggcgtcag tcctactacc gtttctcggg ttatcaataa
    18241 aaaaggatat ctatctgaga aaaccatcca aaaagtcaat gaagccatgc gagaattggg
    18301 ctataaaccc aacaacctgg ctcgtagtct gcaaggaaaa ccagctaagt taatcggctt
    18361 gattttcccc aatatttcca atgttttcta tgcagaattg attgataaat tggaacacca
    18421 actcttcaaa aatggttaca agaccatcat ctgcaacagt gaacatgatt ctgagaagga
    18481 acgtgaatac atcgaaatgt tggaagccaa tcaggtggac ggcatcattt ctggtagtca
    18541 caacctagga atcgaagact acaatcgtgt gacagcgccg attatttcct ttgaccgaaa
    18601 cctatcgcca gacatccctg tcgtctcctc tgacaactat gctggtgggg ttcttgctgc
    18661 ccaaaccttg gtcaagacag gtgcccagtc tatcatcatg attacaggga atgacaattc
    18721 caattcgcca accggactgc gccacgctgg ttttgcatcc gtactcccaa aagctcctat
    18781 tatcaatgtt tccagtgact tttctcccgt cagaaaagaa atggaaatca agaatatctt
    18841 gacccgggaa aaaccagatg ccatttttgc ttcggatgat ttgacagcta ttctggtcat
    18901 taaaatcgct caagaattgg gcatttctgt cccaaaagag ctcaaggtca tcggctatga
    18961 tgggacctac tttatcgaaa attactaccc tcaattggct actatcaagc aacctttgga
    19021 agagattgct tgtctcacta ttgatcttct cttgcaaaag attgaaggca aggaagtcgc
    19081 cacaactggt tacttcttac cagttacgct attaccagga aaaagtattt aaacacaaga
    19141 aaactcagac cgattcgtct gagtttttat gatcttaaat tttcgagata gcgctgggct
    19201 gtctctaggt taaaggtttt atctgagatg aggcgctcta ctaggggagc aacttcagat
    19261 tcactagccc cagctaggag agctagggat ttggcctgta gtttcatgtg gccttgctgg
    19321 atgcccgtac ttaccaaggc tttgagggct gcaaaatttt gagcaagacc gatggacacg
    19381 ataatctggg ctaattctct ggcagaagga tttcctagta gatcatgact gagagctaca
    19441 cgtgggttga ggccgataga gccaccctta gtcgctacag gcatgggcag ggtcatctca
    19501 ccgaccaatt cttctctttc aaggtccagc gtccagcagc taagaccttg atagcgtcca
    19561 tctcgactgg caaaggcatg ggccccagct tcgatggcac gccagtcatt accagtggca
    19621 atcaaaatcg catcaatacc attaaaaatt cctttattat gagtagcagc tcggtaagga
    19681 tcagcctgcg caaactgact agccaacgca attttctccg caatctctcg tccttgatcc
    19741 ttttggcggc tcaagtagcg aaaggcgatg cgacagcttg cagtcaccag agaatcagtc
    19801 gcgtagttgg acaggattcc catgagactc tgtccctgac tgagttcttc taagactggt
    19861 ttcaaggctt ccagcatggt gttgagcata ttggcaccca tggcttcctg ggtatcgaca
    19921 tgaatataaa caacgagaaa gtctggttcg ccttttatct gctcgacatg cagatcacgc
    19981 gccccacccc cacgtttaac gatagaagga taggcttgat tggcaagctc caagagctcc
    20041 gctttcttgc tggcaatctt ctcttgcgct agtttaggat tagcaacttg ataaagggct
    20101 acctgcccaa tcatctggcg ctgatggact tgtgcagtaa aaccacctgc acgcttgatg
    20161 attttgctgg catagctggc cgccgcaacc acagagggtt cttctgtcac atagggaacg
    20221 gtgtattcct gaccgttgac aagtacctcc ggaaccagcg aataaggcag agaaaaagtt
    20281 cccaccacat tctcactcag ctggtctgcc acagtcacac tcatctgttc atccttctcc
    20341 agactagctt gtctctcagg actaaggagc gcctgagctt ttagcagctc gaggcgctct
    20401 tggtatgatt ttttagaaaa tccattccaa cttatcttca ttatttttca accttgctat
    20461 aacggcgttg gtggtcaaga atttcaacca aggcaaaatc ttgattttca tagccagcaa
    20521 actgggcaga gtttgtttca tccaagttta cttcctcaaa aaagaccttt tcatagtctg
    20581 caacggatag ggcagttcgt tggttgagct tgttcaagcg gtctttatcc aaataagctt
    20641 catatccttc aaccaattca ccactgaaga actcagccac agctccactt ccgtaactat
    20701 aaagggcgat tttatcccca gctttcaagc tatctgtatt ttccaagaga gacaaaagtc
    20761 caaggaaaag tgaacctgtg tagatattcc ccaccttttg actgtagaga atagactggt
    20821 caaaatgctt ttgtaagagg tcttttttct cttgaggcag gctcttatcc atgatttttt
    20881 tcaagccttt tagcgctaat ttaggataag gcaagtggaa acaaacagcc gcaaaatcat
    20941 ccaaagtaag ctggtagcgt ttttgatatt caagccaagt cgttttcaaa ctatccaagt
    21001 attgttgggt agaatagaca ccatttacat aaggagttgt cgagtaattt ggtcgccaga
    21061 aatccatgat gtcacgggtc tgagctacat tgtcattatt aaaggccatc atgcgtggat
    21121 tttgtgtaat caacatagct acacttccag caccttgagt tggttctccc ggagtttcaa
    21181 taccatattt ggcaatatca ctggcaatga ccaagacctt ggactccgga gaattttcca
    21241 catgcaattt ggcataatgg agggcagctg tcgccccata gcaggcttct ttaatctcga
    21301 aactacgagc aaagggctgg atgcccagca agccatgcac aaagacggcc gcagccttac
    21361 tctggtcaat tcctgactcg gtagccacaa tgaccatgtc aacttcttgt ctttcttgct
    21421 cagttaaaat agagtcacta gcactggccg ccaaggtcac gatgtcctca gttaggggcg
    21481 caatactcaa ttccttgagt aagagtcctt tacttaattt ttcagggtca attcccctcg
    21541 cttctgctaa gtcttgtaat ttcaagacat attgactggt cgcaaaacca atcttatcaa
    21601 taccgattgt catatttacc tctgttttat cattcatgta aaaaatcgtt ctatactatt
    21661 ttatcacaaa tggcagtaaa agagagaaaa aagacttgat tcaccaaatc aagcctctta
    21721 ttggtcatca ttttaaagaa tgattagttg ctagagagtt caccgatata agtagcttta
    21781 taagctccat tcacagttat cagctcctgg aggatcaaat ttcctgagta aatccttccc
    21841 atctcatcta caaatttttg ataaaactga ctgatcggaa tttctctgac atccttatca
    21901 aatgtcttat caagtgtttt actaaccttc tcagcaatca attgatgctc ttgccatcca
    21961 ctttgaaact ctgagcccga actagaaacc atgactggga taaacaacaa ggtcagtaga
    22021 tttacagaca ataaggaaag tagtagactt cctgcaaaac tagaatccta gttcatgatt
    22081 gataatgcca gcaatcaaat tcattcgtaa tccgaagcgt ttacgatgat ttcgataggt
    22141 tgttgaaaac attttaaacg tttttacttt ggcaaagatg ttctcaacct tgcttctctc
    22201 cttagatagc gcatggttac aggctttatc ttcaactgtt agcggcttga gtttgctgga
    22261 tttacgtgga gtttgtgctt gaggatatat cttcatgagc ccttgataac cactgtcagc
    22321 caagatttta ccagcttgtc cgatatttct gcgactcatt ttgaacaact tcatatcatg
    22381 acaatagttc acagtgatat ccaaagaaac aattctccct tgacttgtga caatcgcttg
    22441 agccttcata gcgtgaaatt tctttttacc agaatcattc gctaattgtt tttttaggac
    22501 aattgatttg tacttccgtc gcatcaatca ttaccgtgtc ttcagaactg agaggagttc
    22561 ttgaaatcgt aacaccacct ttgaacaaga gttacttcaa cccattggct ccgacggagt
    22621 aagttgcttt cgtggatacc aaaatcagcc gcaatttctt cataagtgcg gtattctcgc
    22681 acatatagaa agcgttgtta atttatttat ctcatttttc agaaaattct tttatttctg
    22741 taaagtctac gatactcgat gtgtttttat ataatgatac agtctgagaa tcactgttcc
    22801 gctagccatt ccaatagaga ttaccaaagc caacatgaca accaaggtcg cacttgccag
    22861 tgctttatta tagtcccctg tcacaaaaaa ggcagttgtt cggtaggaga gataacctgg
    22921 aaccagcggt gccaaaatgg ccaagataaa gaccacagca ggtgtcttat aaagaatact
    22981 taaaatctgg ctgacacaag aaccaataat ggctgcaatg aaggtagcta caatgacatt
    23041 ggtcggttcc ttgagcaaga gatagattag ccagacagtc atgcccaaaa tccctccagg
    23101 taagagcata gaccgttgca cattgagtac gattaaaaaa gtgataatgg caagaaaact
    23161 tgctactgct tgtaataaaa aggttgttag tgtcatatta gttcatcaat accaaggcga
    23221 cagaagttcc tgcccctaaa gcgagggtaa tgagcaggga ttcaaacatc ttactcatac
    23281 cagagtttat gtggttggtc ataatatcac ggaccgcatt ggtcaaggca atacctggta
    23341 caaacggcat gaccgcacca gctataatca aatctgccgt tgaaggaaaa cctgtgtagc
    23401 gagcccaaaa ctgggcaatt atcccaaaga caaaggctcc agcaaaggct gtcacaaagg
    23461 gaattcggat aaatttttcc acatagaggg aaaaggcaaa accaaataag gtcgccactc
    23521 ctgccccaag tgcgtcgtag atatttccgc taaacataac tgaaaagaaa ggagcactaa
    23581 aggtcgcagc cagagttacc tgcaacttag tatagggaag gggtttggct tgcaaggccg
    23641 tcaattgctt aaaggctgtt tctaagtcaa tctgcccccc aactagctga cgagaaatct
    23701 ggttcacatc gcagactttt tcgatgttat aagaagagga ggtcacgcgc ttcatgcgcg
    23761 aaatattggt attttcaata gagaaaaaga tagcggcagg catggcaagg acattgcaat
    23821 ccacaatccc ctgcgaatgc gcgattcgaa tcatggtatc ttctacacga tggatttctg
    23881 agccactttt aaggagaata gtccccgcta gcataatcac atcaatgacg gcatttaatt
    23941 cttttgattc ttccatgctt tcctcctttt atcaactccc tctattctat cacaaatccg
    24001 gactcaaaaa aaatctttgc catgaaatca tgacaaagat tgattactca ttttgattat
    24061 ccatctgctt ttaaggagta gctgaagttg ttttaggttt gtagattgaa atcttgactc
    24121 tagtcttatt taggtctacc ttttcacctg ctctaggact ttgttcaaca accatgcctt
    24181 ctacactacc tgcaggcgct gtcgtcactt ctacaacttc tatattagct tccttaatcc
    24241 caacaatttg aatcaaattg ttcttagtaa actccaagct ggaaccaatg taactcggca
    24301 tggcaacact tgtaactttt ttagctactg tcaagacaat ttgagtaggt ttactcacat
    24361 cataagtcgt tccggcacct ggactttgtt tcataatcgt tcctggttcg ctttcgctgg
    24421 actcttcttc ctctatctta atcaaattct caggaacctt cttctgcttg agttctgaga
    24481 ttacttctgt agagttccgt ccaatatagt tccctaattg aatcgtcgta gcttttttag
    24541 ctactgtcaa aacaatttga gttgccttgc tcaagtcata ggtcgtacct tctggtagac
    24601 tttgcttcaa gaccgttcca gcctcactct cattggactc ttcttcctca attttaatca
    24661 aattatctgg aacttttttc tcttttaatt ccgcgataac atcagaagat ttccggccga
    24721 cataattact aatttggaag gattgtttgc ctgatgagac aaccagattg attttcgttc
    24781 cttcttttcg accagttcca gcgccaggat ctgtacggat aatccgccct tcttccacct
    24841 tttcactagc ctctgtcttc tcctcaccaa tctcaaaatt ggcttttttg agcgttgcct
    24901 tggcctctgc aactgtctga cctgccacat ccggaatggc aatggttgca ggagttctgg
    24961 ataagatcca aataagagaa gctgccacca atacaaggct ggccaacaaa atcaggtaac
    25021 gcatcttaaa tctatgtttt ttcggtgctt gtggttggta agtttcctct gtcacagcct
    25081 ggcttgggtt tttgattgat ttgtgttctg tctgcgcttg aaccttagga atagatgtca
    25141 aggtactctg agaaaccttc ggcaaggtct tggtatctgc cttgctcgtt tcatcaaaga
    25201 ttaacttact ttcatttcta cgattgtagg acaagctact agacaagtcc acatacatct
    25261 ctgaaaccga tcggtagcga ttggtcaact ttttagcagt tgccttgata ataacatttt
    25321 ctaaagcctg aggtacagat ggattttctg caataacgga cggcaggggt ttctggaaat
    25381 gctggagggc aatggtcacc gcgctatccc cgtcataagg gatatggcct gtcaacatct
    25441 catagaaaat aatccccatg gcatagatat cactctgcac agtcgcctta gaaccacgcg
    25501 cctgctctgg tgacaagtaa tgaactgagc ccaacatcga gttagtctgg gtcagacttg
    25561 tctctgcaaa ggctacagca atcccaaagt ctgtgacctt ggcagtccca tctggtgtca
    25621 aaaggatatt ttgaggtttc aagtccctgt gaacaattcc tcgagtatgg gccaagcgca
    25681 tagccaagag aatttgtccc atgatacgga cggcttcttc attagaaaga ggataatgtt
    25741 ccttgatata gcgtttgagg tctagtccag caacatactc cattgcaaga tactgttgac
    25801 cgtcttcctc accaatatct gttatccgaa cgatatgagg atggtctaga tctgccatag
    25861 ctctcgcttc acgctgaaaa cgagctacag ctatcgggtc cgtctggtag ttggtcctca
    25921 gaaccttcac tgccacttct tccccatcta agattaagtc tttggctagg taaacatccg
    25981 ccatgcctcc tcgaccaatc tgtttgacaa tccgatagcg tccggcaaaa atcttgccga
    26041 tttggatcat tcttcatcct cctcgttcat agaaacaagg gcaaccgtaa tgttgtctaa
    26101 acctcctgca ttgttagcaa aacgaacaag tgtctccgtt ttatctgcta aaggaatatc
    26161 actggttaca atatcacgaa tctcactgcc tgaaatcatg ttggtcaagc cgtcactatt
    26221 gagcaagaga tagtcacctg actcaaggat aactgtccca aaatcaggct gaatttcatc
    26281 tttttgccca atagactggg tgataatatt tttttgcgga tgagcttctg cctcttctgg
    26341 tgtcaattga ccagccttga gcaattcatt aaccaaggaa tgatcgctcg tcaactgatg
    26401 gtattcttct ccacgaatca agccgatacg cgaatcacca atatgagcat agatagcctg
    26461 attatcaata atagcaagga cttccaaagt agttcccatg cctctgtaag cttcatcctg
    26521 accaagctgg tgaatctttt gattttcaat ttctaggtaa tgggcgaacc attcacgcac
    26581 ttcattgact gtatcgatct gggtatcaac ccaagctaca cccaggtctg tgaccgccat
    26641 ttcactagcg atattccctg cacgatgacc tcccatccca tcagctaaaa taatcatggt
    26701 acgtccagct ctattgacat agtggttgac atagtcttgg ttatttgttc gtttctgacc
    26761 aacatctgtt aataatgaaa tttccatgtg tcagttcctt cctaatccga tatcttgcga
    26821 aattgactga tgaagaatcc atcacttcca tacaattcag gtgtaatgag gatacagccg
    26881 tctttcatga tatccttaca ttcatgttct agttttacct gctcgaactc gggatgactc
    26941 tctaaaaagg ccttaacgac ttgaaaattc tcctctgaga cgatagtgca ggtgctataa
    27001 gttattatac cacctttgcc tagtatttga caaacactac ctaatatttc taactgaatt
    27061 tcctgcaagg acgcgaaatc tgccgtttct ttattgtatt tgatatctgg ttttcggcgc
    27121 aaaagaccga ttcctgaaca aggagcatcc accaaaatct tatcaaagga atcctggtca
    27181 aaaaactcat gcacctttct ggcatccaat ttttgagttt gaacccgatc ttcaactccc
    27241 agacgttggg cattttcttg aattaaatcc aacttgtggt cgtacaagtc cagagcagta
    27301 acctgacctg tcgtaagata agaggctata tgggctgttt tcccacctgg agccgcacag
    27361 gcatcaagca ctcgctcatc accttgtaaa tcaagcgtcg gagcaaccag ctgactggac
    27421 tcgtcttgga tggtaatggc tccatccgca aacaaattat gccctgcaaa atgcccctgc
    27481 tccttaacca gaccagtggt tgctaaaagg gaatcattcg cctccaacaa ggcttggatt
    27541 tcctcttttc gacttaggtc tgttacacga atactggctt tgtttcgcac taacaggctt
    27601 tcaaagatgg cttttgctct ctcctctccg tattcttcct tgagtttggc aactagccaa
    27661 actgggagag aataggcaat ggagtcacgc ttgttttttc gcttgatgct agcaatatct
    27721 ggccagcctt cacgcaagat acggcgaagg agggcattga ccaatttttc actgcctttt
    27781 ttacgaagtt tggccaattc cactgcttca ttgaccacag catgatctgg aatcttgtcc
    27841 aaatagcgga gttggtaggc actcatgaga agaaggacat agagccagct gtctaactgg
    27901 tctctgtctt cgataaagtg ggataggtac cattccagag tcagtttacg ggctaccgtt
    27961 ccatagacca gctcggtcac taagcccttg tctgctgcca aaagttgact cccctttaga
    28021 tgcttattta aggcgatatt tgagtatgct tggttcacaa aaacatcctc tagcactgct
    28081 agagctaaac ttctagccgt ttctacttta gtcaccaaat cgttctccta cagtcaatgt
    28141 acgtccaact ccgttgagga aggaagcaat gtccatctta ggcttaccag ctggctgcac
    28201 ttgtttgagg gatagagccc cttcagccgt tgcgacaatc aattctttct tgccgataga
    28261 gagaatctca cctggatttc cctgaccttc tactggtagg gcttcataaa tcttaaagcg
    28321 gtcgccctta aggaaagcat gggcaacagg ccaggggttc attccacgaa tttggttaaa
    28381 gagttgacga ttggttttgt tccagtccag tttttcttcc tctggcttta tatttggaga
    28441 gaaggtaacc tgactcgtat cctgcggttc aggtttgata tcaccagcaa tataggcagg
    28501 cagagtgtcc aaaagcaaat cacgaccaac tagcgccaat ttttcaaaca aggtgccaac
    28561 attgtcctca tctgtgatcg gaatgctgcg acgagaaatc atatctcctg catccatttc
    28621 cttaaccatt tccatgatgg tcacaccagc ttcctcatcc ccttgaatca aggcataatg
    28681 gataggcgca ccaccacggt gtctaggaag gagggaggca tgaacgttga cagcaaagtc
    28741 catgctatca aggagtttgc ttgggagaaa ctgcccaaaa gcagcagtca caattccatc
    28801 tgctcctagc ttcataagtt cttccatctc tggacttcca gataattttt caggttggta
    28861 gatagatagt cctgcttcct tggcagcctg cttgactggg gtttcttgga taactttttt
    28921 acgaccaaca gcacggtctg gctgggtcac aacggctaga atttcgtaac ggtcatctgt
    28981 caaaagtcct tttaagactg ttgctgaaaa gtcgggggtc cccataaaga ttagttttgt
    29041 catatcttct ccttcttata aaaattgctg cggctcatgg tcaatgctga gacggagctc
    29101 actattttcc cgttcttgag tcaaggccaa gacctggttg agggtcggcc ccagctcatc
    29161 ttctaaacgg tatttaatta aaatctggta atgatagagg ttgtgggtac gggcaatcgg
    29221 ttttggcgtt ggccccagaa tgggactggt ctctgacaag cctgaccgca aaatgttcat
    29281 gacttcatag gcacgtttga aaacctcttc ttctttcttg tgagaaaggg taataccaat
    29341 cgtgaaatag taaggtggat agccgagttg tcgtctgatt cccatttcat aggcataaaa
    29401 gccttcgtaa tcttgatcct tggcaaatcg aatagcatag tgctgcgaat tgtaggactg
    29461 tatcaagact tgacctgctt tttcagcccg acctgctcga cctgccacct gagtcaagag
    29521 ctggaaggtt ctctcagaag aacggaaatc aggcagattc aaggccgtat ccgcatttag
    29581 aactccgact agggtaacat tgggaaaatc caaacccttt gcaatcatct gagtaccaag
    29641 taaaatatcc gcttcccctc gcccaaactg gtcaagcaag gcttggtgac tgcctttctt
    29701 tcgagtcgta tccacatcca tcctcaaaat gcgagcttgg ggaaagagtt ctgctagctc
    29761 atcataagcc ttctgagttc ccgtcccata gtaacgaata ctgcggctct tacagttagg
    29821 acagacctga ggaatatcct tcgagaaacc acaataatgg cagttcatag tcttggtatc
    29881 catatgcaag gtcagagaaa tatcgcagtt gggacaagta tccaccgtcc cacactcccg
    29941 acacatgaca aagctagaat aaccacggcg attgagcatg agaaccacct gctctttttt
    30001 aaccagacgg tcttggatag cctctagcaa aggaggcgta aagtttgacg tctcattttg
    30061 tccgatatag tctcgaaagt caatcacttg aacctcaggg attgtagcca aaggattagc
    30121 acgttgggtt agacgtaagt gttgatagac gcctttgcca gcacgtgccc ggctctctaa
    30181 gctcggcgtt gcagatccaa gtaccagagt tgcttgatta tactgagccc gtaaaatagc
    30241 tacatctctg gcatggtaac ggggattgct gtcctgctta taagccactt catgctcttc
    30301 atcaataatc atgacaccca gatttttcag aggagcaaag atagcagatc tggcaccaac
    30361 aacaacttgg gcatcgccac gctccacctt gcgccattca tcatactttt caccattgga
    30421 taatcctgag tgaagaatgg ctaccttgtc cccaaaacgt gctataaaac gctcggtcat
    30481 ctgaggagtc aaggaaatct caggtaccag caaaatagct gtcttgccct tatccagggc
    30541 accttggata atctgcaagt aaacctcggt cttcccactt cctgtaatcc cttgaagtag
    30601 aaagggaggt tgagaactgc caatagaact cacaaccgca tcacgcgcct gtctttgttc
    30661 tggatttaac tccaaaggtc tacttgcttc aattccttca aaataagcag ccgagcgttg
    30721 aacttccttt tggactatgg taacagcacc ttgatccaca aagaagttga cttgctctcg
    30781 cgagtaggac tctaacaagc tagccaagga agcgctctct ggatgagaca gcagataatc
    30841 tctcagttcc aactttttct tggcacgtgt agaaatctca acaccttcta attgagcatg
    30901 gtcaacctca taccaagact gggtcttgac cttcttttga tcgactgcct gatattccag
    30961 accaagcagg ccttttctag tcaaacgcat catttcagct tgcttggcaa ggtctagtga
    31021 agaaaaggct agcgaatctt ctgaaccaaa caggcgcact cgttcttcct gactcaagcc
    31081 ttccagagga tagagaatct tgtcatagct agaattcaga aatcctggaa gcatggcctt
    31141 gaggatagaa attttgtagg agaagacaga tttgcgtaac tcctcagcca gccagagttg
    31201 ttctggcgtg agaacaggag aaaaatccag cacctctgca atatctttta aatcttgctc
    31261 catctcttct ccatctgatt gggactccaa accaagaaca atcccttgaa tcaggcgatt
    31321 ccccttacca aaaggcacat gaacccgcat cccaacttcc agcattccct caaattcctc
    31381 cggaatcctg taactatagg gctggtccgt ctgcatcaag ggcacatcta cgataatctt
    31441 agctagggcc atcgtctcac ctcctccttg tcagtacatt cttgcaatag aaaaaataag
    31501 attgagtccc cccaacctta aattttttca ccatcttctt tttctttagc aatttgctct
    31561 ttgattttct tttcttcttc ttctttgcgg cgtttttctt cttcgatacg gcgacgcact
    31621 gcttcacgtt ttccttctgg atctgggtga attgtaacgt ttcctgattc gatttcttct
    31681 aaagcgcgaa gagttgattt ttcagactta aaaccttgag ttgctggggc acctgcttcc
    31741 aattcgtggg cacgttttgc ttccaagatt acgagtgaat attttgaagg aaccttgtcg
    31801 agcaaggtat caatagaggg ttttaacatc atttgcttgt acctattttc taaattttat
    31861 cgggtagttg gagattttgg taacatctcc tgatagtgac caatgacacg atccacacag
    31921 aagtgttctg cttcaatcac acatttgaca cgttcagcag ctaggggtac ctgatcgttg
    31981 acaatcgcat aatcatactc acgcatgagg gcaatttctt ccttggcctt ttcgattcgt
    32041 tgggcaatca cttctgcact atctgttcca cgacctacca agcgatcttg caattcatcc
    32101 aaatctggtg gtgtcaggaa gataaagaca gcatctggaa cctttttctt gacctgaaga
    32161 gcaccctgaa cttcaatttc aaggaaaaca tcgattccct tgtccaaggt ttcattgaca
    32221 taggtcagag gagttccata gtagttgccg acatattctg cgtattccaa catctgtcct
    32281 tgacgaatca gctcttcaaa ttcttcacga gtacggaaga aatagtcaac accgtccact
    32341 tctccaggac gttgtgcgcg tgtcgtcatc gatacagaat attgaaattg gttttcagaa
    32401 ctctcaaaaa tctctcttct aaccgttcct tttccaaccc ctgaaggacc agaaaaaacg
    32461 attagtaagc ctcggtctgc cattgtgtct ccttttagtc aatctgtgaa ataacatttc
    32521 tctagaataa tggcaaaaag ccagattatc ctttacagtc tttctatcta gtgtaacaaa
    32581 aaagcagtaa tttttcaact gctctttctt atttatttag cataatctac tgcacgaagc
    32641 tcgcgaatca cggttacctt gatatttcct ggataatcga gattgttttc aattttctta
    32701 cgaactttgt gagccaagat tgtgactttg tcgtccttga tttttcctgg attgaccatg
    32761 atacgaattt cacgtcctgc ttgaagggca aagctagttt gcactccttc aaagccgtta
    32821 gcaatttctt ccaaatcatg gagacgcttg atgtagcttt caagagactc actacgagca
    32881 cctggacggg ctgcgctcaa ggcatctgct gcagcgacga taactgctat cacgctctca
    32941 gcttcaacat ctccgtggtg actagcaatc gtattcacca caactggggg ttccttgtac
    33001 ttacgggcca attccatacc gatttcaacg tggctacctt caacctcatg gtcaatggct
    33061 ttcccgatat cgtgaaggaa tccagcacga cgggcaagag ccgcattttc accaagttcg
    33121 ctcgccatga taccagccaa cttagcaacc tcaatcgaat ggcgcaaaac attttgtcca
    33181 tatgaagtac ggaactgcaa acgtcccata atcttcatca agtctggatg aaggtttggc
    33241 gcaccaattt cataggcagc agcctcaccg tattcacgaa tcttattgtc aatctcttga
    33301 cggtttttct caaccaactc ttcgatacga gctggatgta tacgaccatc tttgagcaac
    33361 atttccatag tcatacgggc aatctcacga cgaatcggat caaatcctga caaggtcacc
    33421 acttctggtg tatcgtcgat aatcacatcg acccctgtca aactttcaaa ggtacgaatg
    33481 ttacgacctt cacgaccaat aatgcgtccc ttcatagtat cgtctggcag atgaactgtt
    33541 gagtttgttg actccgctac atattcacca gcgatacgtt gcatagcttg aaccaagatg
    33601 tccttggcca ttttgtcaga acgttccttg acctcttgct cagcttcgcg aatgcgactg
    33661 gcaatctccc tggtcaagtt ttcctctgtc tgagccaaga taatatctcg tgcttctgcc
    33721 tgagacagag caccaatacg ctctagttct gcttcttttt gtctttcgac ttcctctaat
    33781 tgctcttcac gcgcatcaag gtttttcgct ctatcagaaa tactttgttc tttttgttca
    33841 agtgtttgtt ctttactcgt caaattgtcg tccttacggt caaggctagt agctctctct
    33901 gtcaaacgac tttcgatttg tttgagttct tgacgttctg atttgaattc agcgtccact
    33961 tcttcacggt attttctggc ttcttctttg gcctccaata gtgcttcttt tttaagagac
    34021 ttgctttcac gtttggcttc attaacaagt aaatccgctt cacgctcagc ttgtccacgt
    34081 aaattagttg cttcttgttc agcatttaaa agcatcaact ctgcagcttc ctgagatgat
    34141 ttcatcttag ctgagatgct gacatatcca atgactaaac caatgatgac ggcaaaaaca
    34201 gcaatcgcaa gcgacatgat ttccatgttt ttacctcatt ttattgttat tccgaatgac
    34261 atacattctt ttacattcta ccataaaaaa agaatttaaa caaaccttaa atggatatcg
    34321 ctaacatccg cattatcact atcatagtta tcatttacag atataaagcg aatacctttt
    34381 tcaggaaata tcttttgtaa gtactttcct gactcgatat aatctctgcc gaaacgagat
    34441 aaatctttta ccataatgat agaaaacttt cctttattca ggtcatcaat catattctta
    34501 aaatttggtc gctcaaaatt agaaccacta tatccatcat ctacatattc ataggataat
    34561 tcaatagatt tctcttctac ctccaaaaag caaaaaggat aaatttcccc tacactttaa
    34621 tagtgaaaat ttatccctct tgg
//
LOCUS       DAVLFB010000006        34390 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLFB010000006 DAVLFB010000000
VERSION     DAVLFB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.006"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            565..1017
                     /locus_tag="VXD58_000279"
     CDS             565..1017
                     /locus_tag="VXD58_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0675036.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1036..2373
                     /locus_tag="VXD58_000280"
     CDS             1036..2373
                     /locus_tag="VXD58_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV0675037.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2452..3336
                     /locus_tag="VXD58_000281"
     CDS             2452..3336
                     /locus_tag="VXD58_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0675038.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3336..4169
                     /locus_tag="VXD58_000282"
     CDS             3336..4169
                     /locus_tag="VXD58_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0675039.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4187..6280
                     /gene="nanB"
                     /locus_tag="VXD58_000283"
     CDS             4187..6280
                     /gene="nanB"
                     /locus_tag="VXD58_000283"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV0675040.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6292..7395
                     /locus_tag="VXD58_000284"
     CDS             6292..7395
                     /locus_tag="VXD58_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0675041.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7569..8267
                     /locus_tag="VXD58_000285"
     CDS             7569..8267
                     /locus_tag="VXD58_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0675042.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8286..9803
                     /locus_tag="VXD58_000286"
     CDS             8286..9803
                     /locus_tag="VXD58_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0675043.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9928..11256
                     /locus_tag="VXD58_000287"
     CDS             9928..11256
                     /locus_tag="VXD58_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675044.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11508..12374
                     /locus_tag="VXD58_000288"
     CDS             11508..12374
                     /locus_tag="VXD58_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0675045.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAIMIAIVSF
                     VQFKVLGNDVEY"
     gene            12390..13229
                     /locus_tag="VXD58_000289"
     CDS             12390..13229
                     /locus_tag="VXD58_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0675046.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13501..13953
                     /locus_tag="VXD58_000290"
     CDS             13501..13953
                     /locus_tag="VXD58_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0675047.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNRFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14050..14394
                     /locus_tag="VXD58_000291"
     CDS             14050..14394
                     /locus_tag="VXD58_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675048.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVTALIGRYIKLGTY
                     AVKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14681..15325
                     /locus_tag="VXD58_000292"
     CDS             14681..15325
                     /locus_tag="VXD58_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV0675049.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15494..16411
                     /locus_tag="VXD58_000293"
     CDS             15494..16411
                     /locus_tag="VXD58_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV0675050.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTSSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTAAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEDDRPVVEAAAALIRETKERFL"
     gene            16429..17313
                     /locus_tag="VXD58_000294"
     CDS             16429..17313
                     /locus_tag="VXD58_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0675051.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17329..18180)
                     /locus_tag="VXD58_000295"
     CDS             complement(17329..18180)
                     /locus_tag="VXD58_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV0675052.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18380..20437
                     /gene="pbp2b"
                     /locus_tag="VXD58_000296"
     CDS             18380..20437
                     /gene="pbp2b"
                     /locus_tag="VXD58_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV0675053.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20448..21044
                     /gene="recR"
                     /locus_tag="VXD58_000297"
     CDS             20448..21044
                     /gene="recR"
                     /locus_tag="VXD58_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV0675054.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21220..22263
                     /locus_tag="VXD58_000298"
     CDS             21220..22263
                     /locus_tag="VXD58_000298"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV0675055.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22347..23720
                     /locus_tag="VXD58_000299"
     CDS             22347..23720
                     /locus_tag="VXD58_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV0675056.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            23707..24318
                     /locus_tag="VXD58_000300"
     CDS             23707..24318
                     /locus_tag="VXD58_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0675057.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24409..25113
                     /locus_tag="VXD58_000301"
     CDS             24409..25113
                     /locus_tag="VXD58_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV0675058.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25332..26705
                     /gene="ftsA"
                     /locus_tag="VXD58_000302"
     CDS             25332..26705
                     /gene="ftsA"
                     /locus_tag="VXD58_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV0675059.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26722..27981
                     /gene="ftsZ"
                     /locus_tag="VXD58_000303"
     CDS             26722..27981
                     /gene="ftsZ"
                     /locus_tag="VXD58_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV0675060.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27986..28657
                     /locus_tag="VXD58_000304"
     CDS             27986..28657
                     /locus_tag="VXD58_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV0675061.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELAGLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNIPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28667..29206
                     /locus_tag="VXD58_000305"
     CDS             28667..29206
                     /locus_tag="VXD58_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV0675062.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29206..29469
                     /locus_tag="VXD58_000306"
     CDS             29206..29469
                     /locus_tag="VXD58_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV0675063.1"
                     /translation="MIFLIRMIYNAVDIYSLILIAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29466..30251
                     /locus_tag="VXD58_000307"
     CDS             29466..30251
                     /locus_tag="VXD58_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV0675064.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30260..31048
                     /gene="divIVA"
                     /locus_tag="VXD58_000308"
     CDS             30260..31048
                     /gene="divIVA"
                     /locus_tag="VXD58_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV0675065.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31396..34188
                     /locus_tag="VXD58_000309"
     CDS             31396..34188
                     /locus_tag="VXD58_000309"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV0675066.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10581 a         6072 c         7481 g        10256 t
ORIGIN      
        1 acggtaacaa aactaattta gagaatgaaa tatagagtat ttctctctta aaagtttttg
       61 gtgaaacgag atgtagaaag gagatttagc caaagagtct attagtgcta gaataataga
      121 ttagaattat tttagaaaaa cgaagtgagc agcttataaa ttcaagtccc caaatagatt
      181 catactagta tcttttgcaa aaaataaagg gcgacttcct tcatgaatat caatttcatc
      241 tataaggaag gtagctaatt gaactaactt atttattctg tttgtcgcta gaaaaatcag
      301 acctccttgt gaagattgag gagatactta atgaaaatca aagaagaaac tagcaagcta
      361 gtagcagatt gcccaaaaca ccgctttgag gttgtagata agactgacct atataatcca
      421 aggtgaagcg actgtggttt gaagagattt tcaaagagta taggctagag agtagtgttt
      481 ttatgtcctt ctagtagaaa atgctagaca gaagaatggg gaacttggat aggaaaaata
      541 gattgagaaa ggaggttaga agagatgatt attacaaaaa ttagccgttt aggaacttat
      601 gtgggagtca atccacattt tgcaacatta atagattttc tagaaaaaac aggactagaa
      661 aatttaacag aaggttcgat tgctatcgat ggtaatcgat tgtttgggaa ttgctttact
      721 tatctagcag atggtcaagc aggggctttc tttgaaacac accaaaaata tttggatatt
      781 catttagttt tggaaaacga agaagccatg gctgttacat cgccggaaaa tgtaagcgtt
      841 acccaagaat atgatgaaga gaaagatatt gaattataca cagggaaagt ggaacagttg
      901 gttcagttga gagctggcga atgcctcatc acttttccag aagatttaca tcaacccaag
      961 gttcgtataa atgatgaacc tgtgaaaaaa gttgtcttta aagttgcgat ttcttaatgt
     1021 agaaagagaa gaacgatgaa aaaaatgaga aagtttttat gtctagctgg aattgcgcta
     1081 gcggctgttg ccttggtagc ttgttcagga aaaaaagaag ctacaactag tactgaacca
     1141 ccaacagaat tatctggtga gattacaatg tggcactcct ttactcaagg accccgttta
     1201 gaaagtattc aaaaatcagc agatgctttc atgcaaaagc atccaaaaac gaaaatcaag
     1261 attgaaacat tttcttggaa tgacttctat actaaatgga ctacaggttt agcaaatgga
     1321 aatgtgccag atatcagtac agctcttcct aaccaagtaa tggaaatggt caactcagat
     1381 gctttggttc cgctaaatga ttctatcaag cgtattggac aagataaatt taacgaaact
     1441 gccttaaatg aagcaaaaat cggagatgat tactactctg ttcctcttta ttcacatgca
     1501 caagtcatgt gggttagaac agatttgtta aaagaacata atattgaggt tcctaaaact
     1561 tgggatcaac tctatgaagc ttctaaaaaa ttgaaagaag ctggagttta tggcttgtct
     1621 gttccgtttg gaacaaatga cttaatggca acacgtttct tgaacttcta cgtacgtagt
     1681 ggtggaggaa gcctcttaac aaaagatctt aaagcagact tgacaagcca acttgctcaa
     1741 gatggtatta aatactgggt taaattgtat aaagaaatct cacctcaaga ttctttgaac
     1801 tttaatgtcc ttcaacaagc taccttgttc tatcaaggaa aaacagcatt tgactttaac
     1861 tctggcttcc atatcggagg aattaatgcc aacagtcctc aattgattga ttcgattgat
     1921 gcttatccta ttccaaaaat caaagagtct gataaagacc aaggaattga aacctcaaac
     1981 attccaatgg ttgtttggaa aaattcaaaa catccagaag ttgctaaagc attcttagaa
     2041 gcactttata atgaagaaga ctacgttaaa ttccttgatt caactccagt aggtatgttg
     2101 ccaactatta aggggattag cgattctgca gcctataaag aaaatgaaac tcgtaagaaa
     2161 tttaaacatg ctgaagaagt aattactgaa gctgttaaaa aaggtactgc tattggttat
     2221 gaaaatggac caagtgtaca agctggtatg ttgactaacc aacacattat tgaacaaatg
     2281 ttccaagata tcattacaaa tggaacagat cctatgaaag cagcaaaaga agcagaaaaa
     2341 caattaaacg atttatttga ggctgttcag tagatgtaaa agactagaaa ataggtgggc
     2401 tagtgagctg aaaagctcta gcccaatctt gtaaaagaag ggagaaggag aatggttaaa
     2461 gaacgtaatt taactcgctg gatatttgtt ttgccagcta tgattatcgt aggattactc
     2521 tttgtttatc cgtttttctc gagtattttt tatagcttta ccaataagca tttgattatg
     2581 cctaattata aatttgttgg tttggctaac tataaagctg tgctatcaga tcccaacttc
     2641 tttaatgcgt tctttaattc aattaagtgg accgttttct cattagttgg tcaagtttta
     2701 gtagggtttg tattggcttt agctcttcac agagtacgcc acttcaagaa attatatagg
     2761 accttattga ttgttccttg ggcatttcct accatcgtta ttgccttctc ttggcagtgg
     2821 attctaaacg gggtttatgg ctacttacct aatctaatcg taaaattagg tttaatggaa
     2881 catacacctg catttttgac agatagtaca tgggcattcc tatgtttggt gtttatcaac
     2941 atttggtttg gagcaccaat gattatggtt aatgtgcttt cagctttgca aacagtacca
     3001 gaagaacaat ttgaggctgc taagatagat ggtgcttcaa gttggcaggt gttcaagttt
     3061 atcgtctttc cacatattaa agtggttgta ggacttctag ttgttttgag aactgtatgg
     3121 atctttaata actttgacat tatctacctc attactggtg gtggaccagc caatgctaca
     3181 acgacgcttc caatttttgc ttacaacctg ggttggggaa ctaaattgtt gggtcgtgct
     3241 tcagcagtta cagtattgct ctttatcttc ttggtggcga tttgctttat ctactttgct
     3301 atcatcagta agtgggaaaa ggagggtaga aaataatgaa gaagaaatcc agtatttatt
     3361 tagatattct ctcacatgta cttttagttg gtgcgaccat cgttgcaatt ttcccattgg
     3421 tatggattat catatcttct gtcaaaggga aaggggaatt aactcagtat ccaacacgat
     3481 tttggcctga acagtttaca ttagattatt tcactcatgt tatcaacgat ttgcacttca
     3541 ttgataacat tcgaaacagt ttaatcattg ccttggctac aaccctcatt gcgattatta
     3601 tttctgctat ggcagcctat ggtattgttc gattctttcc taaattggga gcaatcatgt
     3661 cgagactact cgtcattacc tacattttcc caccaatttt gttagcaatt ccctattcaa
     3721 ttgccattgc taaagttggg ttaacaaata gtttatttgg cttgatgatg gtttatctat
     3781 cttttagtgt tccatatgca gtttggctct tagttggatt tttccaaaca gttccaattg
     3841 gaattgaaga agcggctaga attgatggtg caaataaatt tgttacgttt tataaagttg
     3901 tgctaccgat tgtagcacca ggtattgtag caacagctat ttatacattt atcaatgctt
     3961 ggaatgaatt cctgtatgcc ttgattttga ttaacaatac aggaaagatg acagtagcag
     4021 tagcccttcg ttcacttaat ggttcagaaa tactagactg gggagatatg atggcagcgt
     4081 ctgttattgt agttcttcca tcaattattt tcttctctat catccaaaat aagattgcaa
     4141 gtggattatc agaaggatct gtgaagtaga cgaaagaagg aaaaaaatga ataaaagagg
     4201 tctttattca aaactaggaa tttctgttgt aggcattagt cttttaatgg gagtccccac
     4261 tttgattcat gcgaatgaat taaactatgg tcaactgtcc atatctccta tttttcaagg
     4321 aggctcatat caactgaaca ataagagtat agatatcagc cctttgttat tagataaatt
     4381 gtctggagag agtcagacag tagtaatgaa atttaaagca gatgaaccaa actctcttca
     4441 agctttgttt ggcctatcta atagtaaagc aggctttaaa aataattact tttcaatttt
     4501 catgagagat tctggtgaga taggtgtaga aataagagac gcccaaaagg gaataaatta
     4561 tttattttcc agaccagctt cattatgggg aaaacataaa ggacaggcag ttgaaaatac
     4621 actagtattt gtatctgatt ctaaagataa aacatacaca atgtatgtta atggaataga
     4681 agtgttctct gaaacagttg atacattttt gccaatttca aatataaatg gtatagataa
     4741 ggcaacacta ggagctgtta atcgtgaagg taaggaacat tacctcgcaa aaggaagtat
     4801 tggtgaaatc agtctattta acaaagcaat tagtgatcag gaagtttcaa ctattccctt
     4861 atcaaatcca tttcagttaa ttttccaatc aggagattct actcaagcta actattttag
     4921 aataccgaca ctatatacat taagtagtgg aagagttcta tcaagtattg atgcacgtta
     4981 tggtgggact catgattcta aaagtaagat taatattgcc acttcttata gtgatgataa
     5041 tgggaaaacg tggagtgagc caatttttgc tatgaagttt aatgactatg aggagcagtt
     5101 agtttactgg ccacgagata ataaattaaa gaatagtcaa attagtggaa gtgcttcatt
     5161 catagattca tccattgttg aagataaaaa atctgggaaa acgatattac tagctgatgt
     5221 tatgcctgcg ggtattggaa ataataatgc aaataaagcc gactcaggtt ttaaagaaat
     5281 aaatggtcat tattatttaa aactaaagaa gaatggagat aacgatttcc gttatacagt
     5341 tagagaaaat ggtgtcgttt atgatgaaac aactaataaa cctacaaatt atactataaa
     5401 tgataagtat gaagttttgg agggaggaaa gtctttaaca gtcgaacaat attcggttga
     5461 ttttgatagt ggctctttaa gagaaaaaca taatggaaaa caggtcccta tgaatgtttt
     5521 ctacaaagat tcgttattta aagtgactcc tactaattat atagcaatga caactagtca
     5581 gaatagagga gagagttggg aacaatttaa gttgttgcct ccgttcttag gagaaaaaca
     5641 taatggaact tacttatgtc ccggacaagg tttagcatta aaatcaagta acagattgat
     5701 ttttgcaaca tatactagtg gagaactaac ctatctcatt tctgatgata gtggtcaaac
     5761 atggaagaaa tcctcagctt caattccgtt taaaaatgca acagcagaag cacaaatggt
     5821 tgaactgaga gatggtgtga ttagaacatt ctttagaacc actacaggta agatagctta
     5881 tatgactagt agagattctg gagaaacatg gtcggaagtt tcgtatattg atggaatcca
     5941 acaaacttca tatggcacac aagtatctgc aattaaatac tctcaattaa ttgatggaaa
     6001 agaagcagtc attttgagta caccaaattc tagaagtggc cgtaagggag gtcaattagt
     6061 tgtcggttta gtcaataaag aagatgatag tattgattgg aaataccact atgatattga
     6121 tttgccttcg tatggttatg cctattctgc gattacagaa ttgccaaatc atcacatagg
     6181 tgtactgttt gaaaaatatg attcgtggtc gagaaatgaa ttgcatttaa gcaatgtagt
     6241 tcagtatata gatttggaaa ttaatgattt aacaaaataa aggagaaaaa catggttaaa
     6301 tacggtgttg ttggagcagg gtattttgga gctgaattgg ctcgctacat gcaaaagaat
     6361 gatggagcag agattactct tctctatgat ccagataatg cagaggcgat tgcagaagaa
     6421 ttgggagcaa aagtagcaag ttccttagat gagttggttt ctagcgatga agtagattgt
     6481 gttatcgtcg caactccaaa taatcttcat aaggaaccgg ttattaaggc tgcacagcat
     6541 ggtaaaaatg ttttctgtga aaaaccaatt gcgctttctt atcaagattg tcgcgagatg
     6601 gtagatgcgt gtaaagaaaa caatgtaacc tttatggcag gacatattat gaatttcttt
     6661 aatggtgttc atcatgcaaa agaactcatt aatcaaggag ttatcggaga cgttctatat
     6721 tgtcatacag ctcgtaatgg ttgggaagaa caacaaccgt cagtatcatg gaaaaaaatt
     6781 cgtgaaaaat caggtggtca cttgtatcac cacatccatg aattggattg cgttcaattc
     6841 cttatggggg gcatgcctga aactgtaacc atgacaggtg gaaatgtggc ccatgaaggt
     6901 gaacatttcg gtgatgaaga tgatatgatt tttgtcaata tggaattttc taataagcgt
     6961 tttgctttgt tagaatgggg ttcagcttat cgttggggtg aacattatgt cttaatccaa
     7021 ggaagcaaag gtgccatccg cttagactta ttcaactgta aaggaactct taagctagat
     7081 gggcaagaaa gctatttctt gattcacgaa tcgcaagaag aagatgatga tcggactcgt
     7141 atctatcata gtacagagat ggatggagca attgcttatg gtaaaccagg taaacgtact
     7201 ccattatggc tatcatctgt cattgataaa gaaatgcgct atctgcatga gattatggaa
     7261 ggagctccag tatcagaaga atttgcaaaa cttttgacag gtgaagctgc cctagaagca
     7321 attgctactg cagatgcttg tacccagtct atgtttgaag atcgcaaagt aaaattgtca
     7381 gaaattgtaa aataaatttt ggtattctcc tatttatagg tcgacttgct cctctgaaag
     7441 tacttttaga ggagctgttt gactttgcta gtttttgaaa ctgaaatcta ttatactaca
     7501 aactattgaa agcgttttaa ttttaaggta taataatctc atagaaataa agaaaaggag
     7561 gaaagaggat gccacagatt agcaaagaag ccttgattga gcaaatcaaa gatggaatca
     7621 tcgtttcttg tcaggctctt cctcatgaac cgctttatac agaagcggga ggggtgattc
     7681 ccttgctggt caaagcggct gagcaaggtg gagcagtcgg tatccgagca aacagtgttc
     7741 gcgatatcaa ggaaattaag gaagtcacta aacttccaat cattgggatt atcaaacgtg
     7801 attatccacc tcaggaaccc ttcatcacgg ctactatgaa agaagttgat gaattggcag
     7861 aactggacat cgaggtgatt gctctggatt gtaccaagcg tgaacgctac gatggtttgg
     7921 aaattcaaga gttcattcgt caggttaagg agaaatatcc taatcagctt ttgatggctg
     7981 atactagtat cttcgaagaa gggctagcag ctgtagaagc aggaattgac tttgtcggaa
     8041 caaccttatc aggctacaca tcctacagtc caaaagtaga cggtccagat tttgaattga
     8101 ttaagaaact ctgtgatgcc ggtgtagatg tcattgcaga aggaaaaatt catacaccag
     8161 aacaagccaa acaaatcctt gaatatggag tgcgaggcat cgttgttggt ggcgccatta
     8221 ctagaccaaa agagattaca gaacgcttcg ttgctagtct taaataagat gtgaggggga
     8281 gttttatgtt taaagtttta caaaaagttg gaaaagcttt tatgttacct atagctatac
     8341 ttcctgcagc aggtctactt ttggggattg gtggtgcact ttcaaaccca accacgatag
     8401 caacttatcc aatactagac aatagtattt ttcaatcaat attccaagta atgagctctg
     8461 caggagaggt tgtattcagt aatttgtcac tacttctctg tgtgggatta tgtattggct
     8521 tagcgaaacg agataaagga accgctgcgt tagcaggagt aactggttac ttagttatga
     8581 ctgcaacgat caaagctttg gtaaaacttt ttatggcaga aggatctgca attgatactg
     8641 gagttattgg agcattagct gtcggaatag ttgccgtata tttgcacaac cgatataaca
     8701 atattcaatt accttccgct ttaggattct ttggaggttc acgcttcgtt cctattgtta
     8761 catcgttctc ttctatcttg attggctttg tcttctttgt tatttggcca cctttccaac
     8821 aacttcttgt ttctacaggt ggatatattt ctcaggcggg tccaattgga acttttctat
     8881 atggattttt aatgagactt tctggagcag taggcttaca tcatataatt taccctatgt
     8941 tttggtatac tgaacttggt ggtgttgaaa ctattgcagg acaaacagtg gttggagctc
     9001 aaaaaatatt ttttgctcaa ttagccgatc cggcccattc tggattattt acagaaggaa
     9061 caaggttttt tgcaggtcgt ttctcaacaa tgatgttcgg tttaccggct gcctgtttag
     9121 cgatgtacca tagtgttcct aaaaatcgtc gtaaaaaata cgcgggtttg ttttttggag
     9181 ttgctttaac atcttttatt accggtatta cagaaccaat tgaatttatg tttctattcg
     9241 tcagtccggt tctatatgtt gttcacgcat tccttgatgg tgttagcttc tttattgcag
     9301 acgtcttaaa tatttcaata ggaaacacat tttcaggagg tgtaatcgat ttcactttat
     9361 ttggaatttt gcaggggaac gctaagacga attgggttct tcagattcca attggactta
     9421 tttggagtgt tttgtattat attattttta gatggttcat tactcaattc aatgttctaa
     9481 cgccaggacg aggagaagaa gtagattcta aagaaatttc tgaatccgca gattcaactt
     9541 caaatactgc agattattta aaacaggata gcctacaaat tatcagagcc ttgggtggat
     9601 caaataatat agaagatgta gatgcttgtg tgacacgttt acgtgtagct gtaaaagaag
     9661 ttaatcaagt tgataaagca cttttaaaac aaattggtgc agttgatgtc ttagaagtga
     9721 agggtggcat tcaagcaatc tatggagcaa aagcaatctt atataaaaat agtattaatg
     9781 aaattttagg tgtagatgat taagtactta ctgacttaat aaaaaacaga ggagagtgat
     9841 ggatgagtag gatgaaatga aatcgcatac aagaaataaa gaactcatta tccaagttgg
     9901 atacgcttat tacataggag aatacaaatg aaatttagaa aattagcttg tacagtactt
     9961 gcgggtgctg cggttcttgg tcttgctgct tgtggcaatt ctggcggaag taaagatgct
    10021 gccaaatcag gtggtgacgg tgccaaaaca gaaatcactt ggtgggcatt cccagtattt
    10081 acccaagaaa aaactggtga cggtgttgga acttatgaaa aatcaatcat cgaagcgttt
    10141 gaaaaagcaa acccagatat aaaagtgaaa ttggaaacca tcgacttcaa gtcaggtcct
    10201 gaaaaaatca caacagccat cgaagcagga acagctccag acgtactctt tgatgcacca
    10261 ggacgtatca tccaatacgg taaaaacggt aaattggctg agttgaatga cctcttcaca
    10321 gatgaatttg ttaaagatgt caacaatgaa aacatcgtac aagcaagtaa agctggagac
    10381 aaggcttata tgtatccgat tagttctgcc ccattctaca tggcaatgaa caagaaaatg
    10441 ttagaagatg ctggagtagc aaaccttgta aaagaaggtt ggacaactga tgattttgaa
    10501 aaagtattga aagcacttaa agacaagggt tacacaccag gttcattgtt cagttctggt
    10561 caagggggag accaaggaac acgtgccttt atctctaacc tttatagcgg ttctgtaaca
    10621 gatgaaaaag ttagcaaata tacaactgat gatcctaaat tcgtcaaagg tcttgaaaaa
    10681 gcaactagct ggattaaaga caatttgatc aataatggtt cacaatttga cggtggggca
    10741 gatatccaaa actttgccaa tggtcaaaca tcttacacaa tcctttgggc accagctcaa
    10801 aatggtatcc aagctaaact tttagaagca agtaaggtag aagtggtaga agtaccattc
    10861 ccatcagacg aaggtaagcc agctcttgaa taccttgtaa acgggtttgc agtattcaac
    10921 aataaagacg acaagaaagt cgctgcatct aagaaattca tccagtttat cgcagatgac
    10981 aaggagtggg gacctaaaga cgtagttcgt acaggtgctt tcccagtccg tacttcattt
    11041 ggaaaacttt atgaagacaa acgcatggaa acaatcagcg gctggactca atactactca
    11101 ccatactaca acactattga tggatttgct gaaatgagaa cactttggtt cccaatgttg
    11161 caatctgtat caaatggtga cgaaaaacca gcagatgctt tgaaagcctt cactgaaaaa
    11221 gcgaacgaaa caatcaaaaa agctatgaaa caatagtcct tagttattct ataaaaagta
    11281 gttttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat agtgtaagaa
    11341 aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa gttcttacat
    11401 aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt caaaaaatta
    11461 ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg cgggaaacag
    11521 tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt gtgttggctc
    11581 cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa tttgagtttg
    11641 taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa tctctgatta
    11701 acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca ctctttgtag
    11761 catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc gtcttcttcc
    11821 ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt tatgacccac
    11881 tatcagggat tctaaacttt gtccttaagt ccagccacat catcagccaa aacatttctt
    11941 ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg accacttcag
    12001 ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat tcactggttg
    12061 aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ttggaagatt aaatggccaa
    12121 gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca ttccagtgtt
    12181 ttgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt accttgatgt
    12241 actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac acaattggtg
    12301 tcttcttggc aatcatgatt gctatcgtaa gctttgttca atttaaagta cttggaaacg
    12361 acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa ccattaacag
    12421 cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc atctttccat
    12481 tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt attcctcctc
    12541 agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg cagaaccctg
    12601 ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc ttagtctgtg
    12661 caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt caacgcattc
    12721 tatttgctat ctttatcgct gctatggcgc ttccaaaaca agttgtcctt gtaccattgg
    12781 tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc ttgcctttga
    12841 ttggatggcc attcggtgtc ttcctcatga aacagttcag tgaaaatatc cctacagagt
    12901 tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg agtgtagcct
    12961 tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc aatacttgga
    13021 atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc atctcacttg
    13081 gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg gcaggagctg
    13141 cccttgctgc tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc ttcacacagg
    13201 gtattactat gggagcggtc aaaggataat actctgagaa aatctcttca aactacgtca
    13261 gcgtcacctt gccatactta agtattgcct gcggttagct tcctagtttg ttcttcaatt
    13321 ttcattgagt ataggaaaat caatctatca agatacagaa gtatatttta tagatttaga
    13381 gaatatagag gttataagtg tctacaaaat ggagggtatg cagttacttt atgaagtttt
    13441 gtcagacact tataaactta agaatggttt tagttaacta tcagaaacga aggaaagagt
    13501 atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc caatttagac
    13561 aaggctatcg actatctcta ccaacatcgt aaagattcat tcgaattagg aaagtatgag
    13621 attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca agttgagaat
    13681 aatcgatttg aacaccataa gaactatgca gatttgcatt tgctgataga agggcatgaa
    13741 tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga agcgagtgac
    13801 attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca caattttgcg
    13861 attttcttcc caggtgagcc acatcagcca aatggttatg caggcatgga agaaaaggtt
    13921 cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt tttttgtaaa
    13981 gctttgataa tactctacca tgaaattgat ctttgtgagg tagagaaatg agaataaaat
    14041 atttaaaaat tggtatcttc taagtatgct gcaagagcta gtttcttaga tggacagggg
    14101 attacagttg atgagatggc ttggataatt aggggcattg tgactgcatt gattggtaga
    14161 tacataaaat taggtactta tgcagttaag tatggtatta gtatggcgcg ctcgatctta
    14221 agtagggcag ctgcaactgc ggcagcaaga gtaggattat tgactaagat ttttggatgg
    14281 attttacgaa tagctgtgct cgcagctgat gtatatggta attttgccaa caatattgct
    14341 gtagcttggg atgcacatga taaaatccct aacaatggtc gtataaactt ttaaaatgcg
    14401 agaatgaaag cactttgtat ttttttattg tatatgttag cttggacagt gcttgcaatg
    14461 ataatccgtg gagggctaga tggatttgat gaacacactt ggcgtactat tttaattgct
    14521 tcgctgtccg ggatatatat agattatatg tccatagata aaagtagaaa aaagtccaaa
    14581 agaaaaaaga aaaaatagat ttgttcatgg tagggactta tgaaagcttt actgacaaaa
    14641 aagaaaacag tttacaaaga aaaatgatgg aggagcaaac atggcacaaa aaggagtaag
    14701 ccttatcaag gcagcatttg atacagataa ctttctcatg cgttttagtg agaaagtctt
    14761 ggacatcgtg acagccaatc ttctttttgt cgtctcttgt ttgcccatcg tgacgattgg
    14821 agtggctaaa atcagcctct acgagaccat gttcgaagtt aagaagagca gacgggtgcc
    14881 tgtttttaaa atctatctaa gatctttcaa gcaaaatctg aaactaggtc ttcagctggg
    14941 tttaatggag ttaggaattg tgtttcttac cctttcagat ctctatcttt tctggggtca
    15001 aacagctctg cccttccaat tgctgaaagc catttgtcta ggcattctga ttttccttac
    15061 gattgtgatg ttggctagct atccaatcgc ggcacgttat gatttaactt ggaaagaagt
    15121 gttacaaaaa gggcttatct tggctagttt taactttcct tggttcttcc tcatgttagc
    15181 cattcttgtc ctcattgtga tggttcttta tctgtccgcc ttcagtctac tcttaggtgg
    15241 ctcagtcttc ctactttttg ggtttggact attggtcttt atccagactg gattgatgga
    15301 gaaaattttc gcaaaatacc aataggagcc ttatttctga aactactttc aaaggctcca
    15361 aacgctattc tataagcgag aaactaaaat ctaagtgtct ataagatatt gaaagccttt
    15421 tcggtaaagt gtatactaaa gaagtaaaga aaaatctgct ttagcagaaa ataaaaataa
    15481 taggagaaaa tctatgtcag atttgaaaaa atacgaaggt gtcattccag ccttctacgc
    15541 atgttatgat gagcaaggag aagtaagtcc agagcgtacg cgtgccttgg ttcaatactt
    15601 cattgataaa ggtgttcaag gtctttatgt caatggttct tctggtgaat gtatctacca
    15661 aagcgttgaa gatcgcaagt tgattttgga agaagtcatg gcggtagcca aaggtaaatt
    15721 gaccattatt gcccatgttg cttgcaataa tactaaagat agtatggaac ttgctcgcca
    15781 tgctgaaagc ttgggagtag atgctattgc aacgattcca ccaatttact tccgcttgcc
    15841 agaatactca gttgccaaat actggaacga tattagttct gcagctccaa acacagacta
    15901 cgtgatttac aacattcctc aattggcagg ggttgctttg acttcaagcc tttacacaga
    15961 aatgttgaaa aatcctcgtg ttatcggtgt taagaactct tcaatgccag ttcaagatat
    16021 ccaaaccttt gtcagccttg gcggggaaga ccatattgtc tttaacggtc cagatgaaca
    16081 gttcctaggt ggccgcctca tgggtgctaa ggctggtatc ggtggtactt atggtgctat
    16141 gccagaactc ttcttgaaac tcaatcagtt gattgcggat aaagatttag aaacagcgcg
    16201 tgaattgcag tatgctatca acgcaatcat tggtaaactc actgctgcac atggaaatat
    16261 gtacggtgtc atcaaagaag ttttgaagat caatgaaggc ttgaatattg gttctgttcg
    16321 ttcaccattg acaccagtga ctgaagacga tcgtccagtt gtagaagcgg ctgcagcctt
    16381 gattcgtgaa accaaggagc gcttcctcta atctaaaagg aggtatttat gacatactac
    16441 gttgcaattg atatcggtgg aactaacatc aagtatggtt tggttgatca agaggggcaa
    16501 cttcttgaat cacatgaaat gccaacagag gcgcataagg gtggacctca tatcttacaa
    16561 aagaccaaag atatcgtagc tagttattta gaaaaaggcc cagtagcagg tgttgccata
    16621 tcttctgctg ggatggtgga tccggataag ggtgagattt tctatgctgg gccgcaaatc
    16681 cctaactacg caggcaccca gttcaaaaag gaaatcgaag aaagctttac tattccttgt
    16741 gagattgaaa atgatgtcaa ctgtgcaggt cttgctgagg cagtatctgg ttcaggcaag
    16801 ggagcaagtg tgacactttg cttgaccatt ggaaccggta tcggtggttg cttgattatg
    16861 gataggaaag tcttccatgg ttttagtaat tcagcctgtg aagtcgggta tatgcatatg
    16921 caggatggag cttttcagga tctcgcttct acaacagctt tagtgaaata tgtagctgaa
    16981 gcccatggag aagatgttga tcagtggaat ggccgacgta tctttaagga agctactgaa
    17041 ggaaacaaaa tctgcatgga aggtattgac cgtatggttg actatctagg aaaaggtctg
    17101 gcaaatattt gctacgttgc caatccagaa gtggtcattc ttggtggtgg tatcatgggg
    17161 caagaggcta tcctcaaacc taagatccgc acagccttga aagaggcttt ggtaccaagt
    17221 ttagcagaaa aaacacgatt agaatttgcc catcaccaaa atacagcagg gatgttgggt
    17281 gcatattatc attttaagac aaaacaatcc tagtttggct cagccaaact aggattttct
    17341 tacacgtttt tgtctacgat agccgttgag ttttttattt tcccagtagc tattaaagat
    17401 tttttccttg ctttcgcgat tgatttccaa aaagtaggca taaatcaaat cgataaagaa
    17461 gagcatagga agttgagcgg atattcgttg gatataggag ggttggctgt gggtggctac
    17521 aagaacagtc tctgtatagg tctggctatt tttattggga acacttgtaa agagtacagt
    17581 ctttgccccc atctccttgg catctaatag actatctaaa atagaaggag ttgagcctga
    17641 aagtgagaag cccagtacta gacaattttc atccatgatg ctggttgtcc aggcaaagcc
    17701 gtcttggtct gtcaaagctt cgcagaccac acctaatcgc ataaaacgta atttcatttc
    17761 acgggcgacg aggccagaac tccctgttcc aaagaagtag atacgctcag catcttcgat
    17821 tagctgggca attcgttcta gttggatttc gtcaatcaag tcttgtgttt gttccctcat
    17881 attgctataa cttctgagga ctcgtttggt cagtggactg tgcttggaaa cttggttggc
    17941 ttgattttct gcctgatgtt ggtattggaa aataaattct cggtagccag taaagccaca
    18001 ctttttagca aagcgggtca aagcagcttg agaaatatgt aatttttggg tgacttgttg
    18061 agaagataaa tcatctgtaa tcgtttcagc ttgcaaaaaa tagcgagcga tttcttgctc
    18121 taggtctgtc atttcttcaa aatgtgaatc aatgatagtt gcgatatctg gtttgtccat
    18181 agggaaagct cctttacatg agtcatactg gaagactaga tcagggaata gtcacacttc
    18241 attataacac ataatataag gatagataaa taaaaacgca tctctgtttt aaaaacgaaa
    18301 aaatcgaaaa agcttctctc ttttccataa ttttctactc aaattgtggt acaattaaga
    18361 gtaagatttt aagttagaaa tgagactgat ttgtatgaga aaatttaaca gccattcgat
    18421 tccgattcgg cttaatttat tgttttcaat cgtcatttta ctctttatga ccattattgg
    18481 tcgtttgttg tatatgcagg ttttgaacaa ggatttttac gaaaaaaagc tagcctcagc
    18541 tagtcagacc aagattacaa gcagttcagc ccgtggggaa atttatgatg ctagtggaaa
    18601 acctttggta gaaaatacgt taaagcaggt tgtttccttt acgcgtagca ataaaatgac
    18661 ggctacagac ttaaaagaaa cagctaaaaa gttactgact tatgtgagca tcagttctcc
    18721 aaatttgaca gaacgccagc tggcggatta ctatttggct gatcctgaaa tctataaaaa
    18781 aatagtggaa gctctcccaa gcgagaaacg cttggattca gatggcaatc gtctatccga
    18841 atcagaactg tataacaatg cggtcgatag tgtacaaacg agtcaactaa actatacaga
    18901 ggatgaaaag aaagaaatct atctttttag tcagttaaat gctgttggaa actttgcgac
    18961 aggaaccatt gctacagatc ctctaaatga ttctcaggtg gctgttattg cctctatttc
    19021 aaaggagatg cctggcatta gtatttctac ttcttgggat agaaaggttt tggaaacttc
    19081 cctttcttct atagttggga gtgtatccag tgaaaaagct ggtctcccag cggaagaagc
    19141 agaagcctat cttaaaaaag gctattctct aaatgatcgt gtaggaacct cctatttgga
    19201 aaagcaatat gaagagacct tacaaggaaa acgctcggta aaagaaatcc atctggataa
    19261 atatggcaat atggaaagcg tggatacaat tgaggaaggt agtaagggaa acaatatcaa
    19321 actgaccatt gatttggctt tccaagatag cgtggatgct ttactgaaaa gttatttcaa
    19381 ttctgagcta gaaaatggtg gagccaagta ttctgaaggt gtctatgcag tcgcccttaa
    19441 cccaaaaaca ggtgcggttt tgtctatgtc agggattaaa catgacttga aaacgggaga
    19501 gttgacgcct gattccttgg gaacggtaac caatgtcttt gttccaggtt cggttgtcaa
    19561 ggcggcgacc atcagctcag gttgggaaaa tggagtcttg tcaggaaacc agaccttgac
    19621 agaccagtcc attgtcttcc aaggttcagc tcccatcaat tcttggtata ctcaggctta
    19681 cggttcattc cctatcacag cggtccaagc tctggagtat tcatcaaata cctatatggt
    19741 ccaaacagcc ttaggtctta tggggcaaac ctatcaaccc aatatgtttg tcggcaccag
    19801 caatctagag tctgctatgg agaaactgcg ttcaaccttt ggcgaatatg gcttgggtac
    19861 tgcgacagga attgacctac cagatgaatc tactggattt gttcccaaag agtatagctt
    19921 tgctaattac attactaatg cctttgggca gtttgataac tatacgccga tgcagttggc
    19981 tcagtatgta gcaactattg caaataatgg tgttcgtgtg gctcctcgta ttgttgaagg
    20041 catttatggt aataatgata agggaggact gggtgacttg attcagcaac tgcaaccgac
    20101 agagatgaat aaggtcaata tatccgactc cgatatgagc atcttgcacc aaggttttta
    20161 tcaggttgcc catggtacta gtggattgac aactggacgt gccttttcaa atggtgcctt
    20221 ggtatccatt agcggaaaaa caggtacagc cgaaagctat gtggcagatg gtcagcaagc
    20281 aaccaatacc aatgcggtgg cctatgcccc atctgataat ccccaaatcg ctgtcgcagt
    20341 ggtctttcct cataatacca atctaacaaa tggtgtagga ccttccattg cgcgtgacat
    20401 tatcaatctg tatcaaaaat accatccaat gaactagaaa ggaaattatg ctttatccaa
    20461 cacctattgc caagttgatt gacagttatt ctaagttacc aggtatcggg attaagacgg
    20521 ctacgcgtct ggccttttat acgattggga tgtctgctga tgatgtcaat gaatttgcaa
    20581 aaaatctcct ttctgctaag agagaattga catattgttc tatttgtgga cgtttgacag
    20641 acgacgatcc ttgttctatc tgtactgatt cgactcgtga ccagacaaca attttagttc
    20701 ttgaggatag tagagatgtg gcagccatgg aaaatatcca agaataccat ggactctatc
    20761 atgtcctgca tggcctcatt tctcctatga atggtatcag tccggacgat atcaatctta
    20821 agagccttat gactcgtctt atggatagtg aggtttcaga agtgattgtg gcgactaatg
    20881 ctacagcgga tggtgaagcg acctccatgt atctttcacg tttgctcaag ccggctggta
    20941 tcaaggttac gcgtctagca cgaggtctcg ctgtgggagc ggacattgag tatgcggacg
    21001 aagtgacact cttacgagcg attgaaaatc ggacagagtt gtaagtgtag gcaaatttac
    21061 gaactccatt catttataaa aaatcaaaga ggctgaaaat cgttcctatc ggcctctttt
    21121 tgtatagtgt gatgagtaga atcaggttca agttttaaaa aaccaagcaa atatgatata
    21181 ctaaagagcg agtattctag tagaattagg acaaataata tgaaacaaac gattattctt
    21241 ttatatggtg gacggagtgc ggaacgcgaa gtctctgtcc tttcagctga gagtgtcatg
    21301 cgtgcggtca attacgaccg tttcacagtc aagactttct ttatcagtca gtcaggtgac
    21361 tctatcaaaa cgcaggaatt tagtcatgct ccggggcaag aagaccgtct catgaccaat
    21421 gaaaccattg attgggataa gaaagttgca ccaagtgcta tctacgaaga aggtgcagtg
    21481 gtctttccag tccttcacgg gccaatggga gaagatggct ctgttcaagg attcttggaa
    21541 gttttgaaaa tgccttacgt tggttgcaac attttgtcat caagtcttgc catggataaa
    21601 atcacgacta agcgtgttct ggaatctgct ggtattgccc aagttcctta tgtggctatc
    21661 gttgaaggcg atgatgtgac tgctaaaata gctgaagtgg aagaaaaatt ggcttatcca
    21721 gtcttcacta agccgtcaaa catggggtct agtgtcggta tttctaagtc tgaaaaccaa
    21781 gaagaactcc gtcaagcctt aaaacttgcc ttccgatatg acagccgtgt cttggttgag
    21841 caaggagtga atgcccgtga aattgaggtt ggcctcttgg gtaactacga tgtcaagagc
    21901 acgctacctg gagaagttgt caaggacgtt gccttttatg actacgatgc caagtatatt
    21961 gataacaagg ttactatgga tattcctgcc aaaatcagtg atgatgtggt ggctgtcatg
    22021 cgtcaaaatg cagaaacagc cttccgtgcc attggtggcc ttggtctatc tcgttgcgat
    22081 ttcttctata cagataaggg agagattttt ctcaacgagc tcaataccat gccaggtttc
    22141 acccagtggt ctatgtaccc actactttgg gaaaatatgg ggatcagcta cccagaacta
    22201 atcgagcgtt tggttgacct tgccaaggaa agttttgaca agcgcgaagc gcatttgata
    22261 taaaatgaaa gagagggtag aagccagaac catcactgca aggtgactag agttctcgga
    22321 cttcagccct ttttaaagga gtagaaatga aattaacaat ccatgaaatt gcccaagttg
    22381 ttggagccaa aaatgatatc agtatctttg aggacactca gttagaaaaa gctgagtttg
    22441 atagtcgttt gattggaact ggagatttat ttgtgccact taaaggtgcg cgtgatggcc
    22501 atgactttat tgaaacagcc tttgaaaatg gtgcagcagt aaccttgtct gagaaagagg
    22561 tctcaaatca tccttacatt ttagtagatg atgttttgac agcctttcaa tccctagcat
    22621 cctactatct tgaaaaaacg actgttgatg tctttgctgt tacaggttca aatggcaaga
    22681 caacgactaa ggatatgttg gcgcatttac tgtcaacaag atacaagacc tacaaaacac
    22741 aaggcaatta caataatgag attggccttc cttacacagt tcttcatatg cctgaaggaa
    22801 cagaaaagtt ggttttggag atgggacagg atcacttggg agatattcat ctcttgtctg
    22861 aattggctcg tccaaaaaca gccatcgtga ccttggttgg agaagctcat ttggcctttt
    22921 tcaaagatcg ttctgagatt gctaaaggaa aaatgcaaat tgcagacgga atggcttcag
    22981 gttccttgct tttagcgccg gctgacccta tcgtagagga ctatttgcca attgataaaa
    23041 aggtggttcg ttttggtcaa ggagcagagc tggaaattac tgacttggtt gagcgtaagg
    23101 acagtctgac ctttaaggct aatttcttgg aacaagccct tgatttgcca gtaactggca
    23161 agtacaatgc gacaaatgct atgattgcat cctatgttgc cttgcaagaa ggagtttcag
    23221 aggagcaaat tcgtttggca ttccaacatc ttgaattgac gcgtaaccgt accgagtgga
    23281 agaaagcagc caatggagca gatatcctgt cagatgttta caatgccaat ccaactgcta
    23341 tgaaactgat tttagagact ttctctgcca ttccagccaa tgaaggtggc aagaaaattg
    23401 cagtgttggc ggatatgaag gagcttggtg accagtctgt tcaacttcat aatcagatga
    23461 ttttgagcct ctccccagat gtgcttgata tcgttatttt ctatggagaa gatattgctc
    23521 agttggctca attggccagt caaatgtttc caatcggcca cgtttactac ttcaagaaaa
    23581 cagaagacca ggatcaattt gaagacctag tcaagcaggt taaggaaagc cttggagcct
    23641 atgaccaaat cttgctaaaa ggttctaact ctatgaatct agccaaattg gtagaaagtt
    23701 tagaaaatga atgcaagtga ttttgctaag tatcttcaaa gaatgattgc tcttacagat
    23761 actggactaa cctttacaaa ggatccgttt gaccgtgagc gctacgaaga cttgcgaagt
    23821 ctgttatctg aaatgttgaa tcaggtatca gaccttgatg cagacgaagt ggcagaactc
    23881 ttgaaaccga cctccgctta tgcgactcct ttaatggacg tccgtgcttg gatagttgag
    23941 gatgaaaaaa tttgtctggt taggggacaa ggagagaatg attgggcttt gccaggtggc
    24001 tttggcgagg tcggttattc accaactgaa aatattctca aggaaattga agaagaaacc
    24061 ggttttaaag ccaaagttga acgactacta gctgtttttg ataccaatcg tttccaacta
    24121 cagagcaaac aatatgcaaa gtttgttttt gaatgtaagc ttctcgatgg acaatttcaa
    24181 gaaaatcaag aaatcgctga ccttcaattt tttgcaattg accaactgcc gaatttatct
    24241 gaaaaacgca ttaccaagga gcaaatagag cttctttggc aggtttatca aggtcatagg
    24301 gggcaatatc ttgactaaga agatgattat cgtatttcta aatccatttt taacaactag
    24361 catggtataa taatatgcag gaaaattttg aattatgagg aagactagat gaatttatgg
    24421 gatattttct ttacgactca ggcaaccgag ccgcccaaat ttgacctttt ttggtatgtt
    24481 agcctattta cgctcttagc cttaaccttt tatacagccc atcgctatcg tgaaaataag
    24541 gtttaccaac gatttttcca aatcttgcag actgttcagt taatccttct ttatggttgg
    24601 tactgggtca atcatatgcc actgtcagaa agcctaccct tttaccattg ccgtatggct
    24661 atgtttgtgg tactcttgct tcctggtcaa tccaaatata aacaatactt tgcattattg
    24721 ggaacatttg ggacattagc agcctttgtt tatccagtgc cagatgctta cccttttcca
    24781 catatcacca ttctatcctt tatctttggt catttagcac tcttggggaa ctctctagtt
    24841 tatctattga gacagtataa tgcgcgattg ctggatgtga agggaatttt tctcatgacc
    24901 tttgccctaa atgccttgat ttttgtggtc aatttggtga caggtggcga ttacggattt
    24961 ttgacaaaac cgccattggt tggggatcac ggtctagtag ctaattattt acttgtttca
    25021 attgtgctgg tagctactat cagtttgact aagaaaatct tagaattctt tttagctcaa
    25081 gaagcagaaa aaatgattgc aaaggaagct taacacagag ctttcttttt tgctcttaga
    25141 gagtttttac aagcagctta taaaataaga atttctgaat agacaaactc aaaaaatggc
    25201 tgggaaattt aggaaaaaag caagcacgat taaatttttt gtgttataat attttgtgaa
    25261 tagctatgcc tatgtttagc tatggaataa tacgaagtgc gaaacttgga agatagagag
    25321 gaagcgatgt aatggctaga gaaggctttt ttacaggtct agatattgga acaagctctg
    25381 tcaaggtgct tgtggccgag cagagaaatg gtgaattaaa tgtaattggc gtgagtaatg
    25441 ccaaaagtaa aggtgtaaag gatggaatta ttgttgatat tgatgcagca gcaactgcta
    25501 tcaagtcagc catttcccaa gcggaagaaa aggcaggcat ttcgattaaa tcagtgaatg
    25561 tcggcttgcc tggtaatctt ttgcaggtag aaccaactca ggggatgatt ccagtaacat
    25621 ctgatactaa ggaaattacg gatcaagatg ttgaaaatgt tgtcaaatca gctttgacaa
    25681 agagtatgac acctgaccgt gaagtcatta cctttattcc tgaagaattt attgtggatg
    25741 gtttccaagg gattcgtgac ccacgtggca tgatgggggt tcgccttgaa atgcgtggtt
    25801 tgctttatac aggacctcgt actatcttgc acaatttgcg taagacggtt gagcgtgcag
    25861 gtgttcaggt tgaaaatgtt atcatttcac cactagcaat ggttcagtct gttttgaacg
    25921 aaggggaacg tgaatttggt gctacagtga ttgatatggg ggcaggtcaa acgactgtcg
    25981 ctacaatccg taatcaagaa ctccagttca cacatattct ccaagaaggt ggagattatg
    26041 taactaaaga tatctccaag gttttgaaaa cctctcgcaa attagcggaa ggcttaaaac
    26101 tgaattacgg ggaagcctat ccgcctcttg caagcaaaga aaccttccaa gtagaggtta
    26161 ttggagaagt agaagcagtc gaagtgacgg aagcctactt gtcagaaatt atttctgcac
    26221 gaatcaagca catccttgaa caaatcaagc aagaattaga tagaaggcgt ctattggacc
    26281 tccctggtgg tattgtctta atcggtggga atgccatttt accaggtatg gttgagcttg
    26341 ctcaggaagt ctttggcgtc cgtgtcaagc tttatgttcc aaatcaagtt ggtatccgta
    26401 atccagcctt tgcgcatgtg attagtttat cagaatttgc gggtcaatta acagaagtta
    26461 atcttttggc tcagggagcg ataaaaggtg agaatgactt aagtcatcag ccaattagtt
    26521 ttggtgggat gctgcaaaaa acagctcagt ttgtacaatc aacgcctgtt caaccagctc
    26581 ctgctccaga agtagagccg gtggcgccta cagaaccaat ggcggatttc caacaagctt
    26641 cacaaaataa accgaaatta gcagatcgtt tccgtggctt gatcggaagc atgtttgacg
    26701 aataaagagg aaaaataaat tatgacattt tcatttgata cagctgctgc tcaaggggca
    26761 gtgattaaag taattggtgt cggtggaggt ggtggcaatg ccatcaaccg tatggtcgac
    26821 gaaggtgtta caggcgtaga atttatcgca gcaaacacag atgtacaagc attgagtagt
    26881 acaaaagctg agactgttat tcagttggga cctaaattga ctcgtggttt gggtgcagga
    26941 ggtcaacctg aggttggtcg taaagccgct gaagaaagcg aagaaacact gacggaagct
    27001 attagtggtg ccgatatggt cttcatcact gctggtatgg gaggaggctc tggaactgga
    27061 gctgctcctg ttattgctcg tatcgccaaa gatttaggtg cgcttacagt tggtgttgta
    27121 acacgtccct ttggttttga aggaagtaag cgtggacaat ttgctgtaga aggaatcaat
    27181 caacttcgtg agcatgtaga cactctattg attatctcaa acaacaattt gcttgaaatt
    27241 gttgataaga aaacaccgct tttggaggct cttagcgaag cggataacgt tcttcgtcaa
    27301 ggtgttcaag ggattaccga tttgattacc aatccaggat tgattaacct tgactttgcc
    27361 gatgtgaaaa cggtaatggc aaacaaaggg aatgctctta tgggtattgg tatcggtagt
    27421 ggagaagaac gtgtggtaga agcggcacgt aaggcaatct attcaccact tcttgaaaca
    27481 actattgacg gtgctgagga tgttatcgtc aacgttactg gtggtcttga cttaaccttg
    27541 attgaggcag aagaggcttc acaaattgtg aaccaggcag caggtcaagg agtgaacatc
    27601 tggctcggta cttcaattga tgaaagtatg cgtgatgaaa ttcgtgtaac agttgtcgca
    27661 acgggtgttc gtcaagaccg cgtagaaaag gttgtggctc cacaagctag atctgctact
    27721 aactaccgtg agacagtgaa accagctcat tcacatggct ttgatcgtca ttttgatatg
    27781 gcagaaacag ttgaattgcc aaaacaaaat ccacgtcgtt tggaaccaac tcaggcatct
    27841 gcttttggtg attgggatct tcgccgtgaa tcgattgttc gtacaacaga ttcagtcgtt
    27901 tctccagtcg agcgctttga agccccaatt tcacaagatg aagatgaatt ggatacacct
    27961 ccatttttca aaaatcgtta agtaaatgaa tgtaaaagaa aatacagaac ttgtttttcg
    28021 agaagttgca gaggctagtc tgagtgctca tcgagagagt ggttcggtct ctgtcattgc
    28081 agttaccaag tatgtagatg taccgacagc ggaagccttg cttccgctag gtgttcatca
    28141 tatcggtgaa aatcgtgtag ataagtttct ggaaaaatat gaagctttaa aagatcgaga
    28201 tgtgacttgg catttgattg gtaccttgca aagacgtaag gtgaaagatg tcattcaata
    28261 cgttgattat ttccatgcat tggactcagt aaagctagca ggggaaattc aaaaaagaag
    28321 tgaccgagtc atcaagtgtt tccttcaagt aaatatttct aaagaagaaa gcaaacacgg
    28381 tttttcgaga gaggaactgc tggaaatctt gccagagtta gccggactag ataagattga
    28441 atatgttggt ttaatgacga tggcaccttt tgaggctagc agtgagcagt tgaaagagat
    28501 tttcaaggcg gcccaagatt tacaaagaga aattcaagag aaacaaattc caaatatacc
    28561 tatgaccgag ttaagtatgg gaatgagtcg tgattataaa gaagcgattc aattcggttc
    28621 cacttttgtt cgtataggta catcattttt taagtaggag agaaccatgt ctttaaaaga
    28681 tagattcgat agatttatag attattttac ggaggatgag gattcaagtc tcccttatga
    28741 aaaaagagat gagcctgtgt ttacttcagt aaattcttca caggaaccgg ctctcccaat
    28801 gaatcaacct tcacagtcgg ctggtacaaa agagaacaat atcaccagac ttcatgcaag
    28861 acaacaggaa ttggcaaatc agagtcagcg tgcaacggat aaggtcatta tagatgttcg
    28921 ttatcctaga aaatatgagg atgcaacaga aattgttgat ttattggcag gaaacgaaag
    28981 tatcttgatt gattttcagt atatgacaga ggtgcaggct cgtcgttgtt tggactattt
    29041 ggatggagct tgtcatgttt tagctggaaa tttgaaaaag gtagcttcta ccatgtattt
    29101 gttaacacca gtgaacgtta ttgtaaatgt tgaagatatc cgtttaccag atgaagatca
    29161 acagggtgag ttcggttttg atatgaagcg aaatagagta cgataatgat ttttttaatt
    29221 cgtatgattt ataatgcagt ggatatttac tccctgattt tgatagcctt cgctgtcatg
    29281 tcttggtttc caggtgccta cgaatccagt ttaggtcgtt ggattgtagc gttggtgaaa
    29341 ccagtgcttg ctcccttgca acgcctgcct ttacagatag cgggtcttga tttatctgtt
    29401 tgggttgcga ttgttttggt tcgattttta ggagaaaact tagtgcgttt tctggcgatg
    29461 ataggatgaa taaagggatt tatcagcatt tctccataga agatcgtcca tttcttgaca
    29521 agggaatgga atggataaag aaggtagaag atagctatgc tcctttttta actcctttta
    29581 tcaatcctca tcaggagaag ctattaaaga ttttggcaaa aacctatggt cttgcttgta
    29641 gcagtagtgg ggaattcgtc tcgagtgagt atgttcgagt tttattatac ccagattatt
    29701 tccaaccaga gttttcagat tttgaaatat ctcttcagga aattgtgtat tccaataaat
    29761 ttgaacattt aacgcatgct aagattttag ggacagtcat caatcaatta gggattgaac
    29821 ggaaactttt tggagatatc ctagtagatg aagaacgggc gcagattatg attaatcagc
    29881 agtttcttct tctctttcaa gatggactaa agaaaattgg tcgtatacct gtttcgctgg
    29941 aggaacgtcc tttcaccgag aaaatagata agctagaaca gtatcgagaa ctggatttat
    30001 ctgtgtctag ttttcgatta gatgttcttt tatcaaatgt tttgaaacta tctaggaatc
    30061 aagcaaacca gttgattgaa aagaaacttg tccaagtaaa ttatcatgtg gtagacaaat
    30121 cagattacac tgttcaagtt ggagacttga ttagtgtgag aaaatttggt cgcttgagat
    30181 tacttcaaga taagggacaa acgaaaaaag agaagaaaaa aataaccgtc cagttattat
    30241 taagtaagtg aggaatagaa tgccaattac atcattagaa ataaaggaca agacttttgg
    30301 aactcgattc agaggttttg atccagaaga agtcgatgaa tttttagata ttgtggttcg
    30361 tgattacgaa gatcttgtgc gtgcgaatca tgataaaaat ttgcgtatta agagtttaga
    30421 agagcgtttg tcttactttg atgaaataaa agattcattg agccagtctg tattgattgc
    30481 tcaggataca gctgagagag tgaaacaggc ggcgcatgaa cgttcaaaca atatcattca
    30541 tcaagcagag caagatgcgc aacgcttgtt ggaagaagct aaatataagg caaacgagat
    30601 tcttcgtcaa gcaactgata atgctaagaa agtcgctgtt gaaacagaag aattgaagaa
    30661 caagagccgt gtcttccacc aacgtctcaa atctacaatt gagagtcagt tggctattgt
    30721 tgaatcttca gattgggaag atattctccg tccaacagct acttatcttc aaaccagtga
    30781 tgaagccttt aaagaagtgg ttagcgaagt acttggagaa ccgattccag ctccaattga
    30841 agaagaacca attgatatga cacgtcagtt ctctcaagca gaaatggaag aattacaagc
    30901 tcgtattgag gtagccgata aagaattgtc tgaatttgaa gctcagatta aacaggaagt
    30961 ggaaactcca actcctgtag tgagtcctca agttgaagaa gagcctctgc tcatccagtt
    31021 ggcccaatgt atgaagaacc agaagtagct ccagtgcatc cgacaggtcc aacaccagct
    31081 acagaaactg ttgattcagc accaggattt gaagcaccgc aagaatccgt tacaatttta
    31141 taagaaacga tgtgagaaca atatcttatc cttatatttc cagcgagcag gagatggtgt
    31201 gagtcctgca ttccctaacg ataagattat cctctcaaaa actcaagtct gaagctagta
    31261 agatttgacg tttcccacgt tacgggataa gagggagaaa gactaaatct ttttccgaat
    31321 aaaggtggta ccacgatttt cgtccttttt ggaagtcgtg gtttttaatt tgttattatt
    31381 tataaaggag ataccatgaa actcaaagat acccttaatc ttgggaaaac agctttccca
    31441 atgcgtgcag gccttccgac caaagagcca gtttggcaaa aggaatggga agatgcaaaa
    31501 ctttaccaac gtcgtcaaga actaaatgaa gggaaaccgc attttgtctt gcatgatggc
    31561 cctccatacg caaacggaaa tatccacgtt ggacatgcca tgaatcatat ctcaaaagat
    31621 atcatcattc gttctaagtc tatgtcaggt tttaacgcac cttatatccc aggttgggat
    31681 actcatggtc tgccaatcga gcaagttttg gcaaagcaag gtgtgaagcg caaagaaatg
    31741 gacttggttg agtacttgaa actctgtcgt gagtacgctc tttctcaagt ttataaacaa
    31801 cgtgatgatt tcaaacgctt ggggatgtca ggtgactggg aaaatttata tgtgacttta
    31861 actccagact atgaagcagc ccaaatccgt gtctttggtg agatggctaa taagggttat
    31921 atctaccgtg gtgccaagcc agtttactgg tcatggtcat ctgagtcagc ccttgccgaa
    31981 gcggaaattg aataccatga tttggtttcg acttcacttt attatgccaa taaggtaaaa
    32041 gatggcaaag gtatcctaga tacagatact tatatcgtag tctggacaac aactccattt
    32101 accatcacag cttctcgtgg tttgactgtt ggtgcagata ttgattacgt tttggttcaa
    32161 cctgctagtg aaactcgtaa gtttgtcgtt gctgcagaat tgttgactag cttgtctgag
    32221 aaatttggct gggctgatgt tcaagtgttg gcaacttacc gtggtcaaga actcaaccac
    32281 atcgtaacag aacacccatg ggatacagct gtagatgagt tggtaatcct tggtgatcac
    32341 gttaccactg actctggtac aggtatcgtc catacagccc ctggttttgg tgaggacgac
    32401 tacaatgttg gtattgctaa tggtcttgaa gtcgcagtga ctgttgacga acgcggtatc
    32461 atgatggcga atgctggtcc tgagtttgaa ggtcagttct acgacaaggt tgtaccaact
    32521 gttattgaaa aacttggtaa cctccttcta gcccaagaag aaatctctca ctcataccca
    32581 tttgactggc gtactaagaa accaatcatc tggcgtgcag ttccacaatg gtttgcctca
    32641 gtttcaaaat tccgccaaga aatcttggat gctattgata aagttaaatt ccatacagaa
    32701 tggggtaaag tacgtctata taatatgatt cgtgaccgtg gtgactgggt tatctctcgt
    32761 caacgtgctt ggggtgttcc acttccaatc ttctatgcag aagacggtac agctatcatg
    32821 acagctgaga ctattgaaca tgtggcgcaa ctctttgaag tacatggttc aagcatttgg
    32881 tgggaacgtg atgccaaaga cctcttacca gaaggattta cacatccagg ttctccaaat
    32941 ggggagttca agaaagaaac agatattatg gatgtatggt ttgactcagg ttcatcatgg
    33001 aatggtgttt tggtaaatcg tccaaatctg acttatccag ctgaccttta ccttgaaggt
    33061 tctgaccaat accgtggttg gttcaactca tcactcatta catctgttgc caaccatggc
    33121 gtagcacctt acaaacaaat cttgtcacaa ggttttaccc ttgatggtaa aggtgagaag
    33181 atgtctaaat cacttggaaa taccattgct ccaagtgatg ttgaaaaaca atttggtgca
    33241 gaaatcttgc gtctctgggt aacaagtgtt gactcaagca atgacgtgcg tatctctatg
    33301 gatattttga gccaagtttc tgaaacttac cgtaagattc gtaacactct tcgtttcttg
    33361 attgccaata catctgactt taacccagct caagatgtag tcgcttacga tgaacttcgt
    33421 tcagttgata agtacatgac tattcgcttt aaccagcttg tcaagaccat tcgtgatgcc
    33481 tatgcggact ttgaattctt gacaatctac aaggccttgg tgaactttat caacgttgac
    33541 ttgtcagcct tctaccttga ttttgccaaa gatgttgttt acattgaagg tgccaaatca
    33601 ttggaacgcc gtcaaatgca gactgttttc tatgacattc ttgtcaaaat caccaaactc
    33661 ttgacaccaa tccttcctca cactgcggaa gaaatctggt catatcttga gtttgaagct
    33721 gaagacttcg ttcaattgtc agaattgcca gaagctcaaa cttttgctaa tcaagaagaa
    33781 gtcttggata catgggcagc cttcatggac ttccgtggac aagctcaaaa agccttggaa
    33841 gaagctcgta atgcaaaagt tatcgggaaa tcgcttgaag cacacttgac agtttatcca
    33901 aatgaagttg tgaaaactct actcgaagca gtaaacagca atgtggctca acttttgatc
    33961 gtgtcagact tgaccatcgc agaagggcca gctccagaag ctgcccttag cttcgaagat
    34021 gtagccttca cagttgaacg cgctgcaggt caagtatgtg accgttgccg tcgtatcgac
    34081 ccaacaacag cagaacgcag ctaccaggca gtcatctgtg accactgtgc aagcatcgta
    34141 gaagaaaact ttgcggaagc agtcgcagaa ggatttgaag agaaataagg ttgaaaagtc
    34201 taggcaaaat tcaatttgag aagaaaagac aactaatttt atagtctatt aaacgcattg
    34261 tatcacgttt ttgaatacct gatatgatgc gttttttatt tattttaaaa atttgcgagg
    34321 tatgactttt tatactcaac aagaatcaaa gagaaactta gcaagctaac agtagtaaga
    34381 taaaatagga
//
LOCUS       DAVLFB010000007        34336 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLFB010000007 DAVLFB010000000
VERSION     DAVLFB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.007"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            357..1520
                     /locus_tag="VXD58_000310"
     CDS             357..1520
                     /locus_tag="VXD58_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV0675067.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1517..2194
                     /locus_tag="VXD58_000311"
     CDS             1517..2194
                     /locus_tag="VXD58_000311"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV0675068.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2184..3038
                     /locus_tag="VXD58_000312"
     CDS             2184..3038
                     /locus_tag="VXD58_000312"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV0675069.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3057..4124
                     /locus_tag="VXD58_000313"
     CDS             3057..4124
                     /locus_tag="VXD58_000313"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV0675070.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4134..5300
                     /gene="aroC"
                     /locus_tag="VXD58_000314"
     CDS             4134..5300
                     /gene="aroC"
                     /locus_tag="VXD58_000314"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV0675071.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5310..6413
                     /locus_tag="VXD58_000315"
     CDS             5310..6413
                     /locus_tag="VXD58_000315"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV0675072.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6424..6762
                     /locus_tag="VXD58_000316"
     CDS             6424..6762
                     /locus_tag="VXD58_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV0675073.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6854..8137
                     /gene="aroA"
                     /locus_tag="VXD58_000317"
     CDS             6854..8137
                     /gene="aroA"
                     /locus_tag="VXD58_000317"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV0675074.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8130..8606
                     /locus_tag="VXD58_000318"
     CDS             8130..8606
                     /locus_tag="VXD58_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV0675075.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8603..9451
                     /gene="pheA"
                     /locus_tag="VXD58_000319"
     CDS             8603..9451
                     /gene="pheA"
                     /locus_tag="VXD58_000319"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0675076.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9448..10722
                     /gene="psr"
                     /locus_tag="VXD58_000320"
     CDS             9448..10722
                     /gene="psr"
                     /locus_tag="VXD58_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV0675077.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11001..11846
                     /locus_tag="VXD58_000321"
     CDS             11001..11846
                     /locus_tag="VXD58_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0675078.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11836..12939
                     /locus_tag="VXD58_000322"
     CDS             11836..12939
                     /locus_tag="VXD58_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0675079.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12941..13927
                     /locus_tag="VXD58_000323"
     CDS             12941..13927
                     /locus_tag="VXD58_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0675080.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13943..15376
                     /locus_tag="VXD58_000324"
     CDS             13943..15376
                     /locus_tag="VXD58_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV0675081.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15405..16439
                     /locus_tag="VXD58_000325"
     CDS             15405..16439
                     /locus_tag="VXD58_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV0675082.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16639..17376
                     /gene="mecA"
                     /locus_tag="VXD58_000326"
     CDS             16639..17376
                     /gene="mecA"
                     /locus_tag="VXD58_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV0675083.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17527..18813
                     /locus_tag="VXD58_000327"
     CDS             17527..18813
                     /locus_tag="VXD58_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV0675084.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18815..19684
                     /locus_tag="VXD58_000328"
     CDS             18815..19684
                     /locus_tag="VXD58_000328"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV0675085.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19760..19834
                     /locus_tag="VXD58_000329"
                     /pseudo
     CDS             19760..19834
                     /locus_tag="VXD58_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19979..20917
                     /gene="msrB"
                     /locus_tag="VXD58_000330"
     CDS             19979..20917
                     /gene="msrB"
                     /locus_tag="VXD58_000330"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0675086.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20975..22699
                     /locus_tag="VXD58_000331"
     CDS             20975..22699
                     /locus_tag="VXD58_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675087.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22701..24449
                     /locus_tag="VXD58_000332"
     CDS             22701..24449
                     /locus_tag="VXD58_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675088.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDTFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24796..26055
                     /locus_tag="VXD58_000333"
     CDS             24796..26055
                     /locus_tag="VXD58_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV0675089.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26131..26266
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26305..26805
                     /locus_tag="VXD58_000334"
     CDS             26305..26805
                     /locus_tag="VXD58_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV0675090.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26881..27249
                     /gene="rplL"
                     /locus_tag="VXD58_000335"
     CDS             26881..27249
                     /gene="rplL"
                     /locus_tag="VXD58_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV0675091.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27436..27606
                     /locus_tag="VXD58_000336"
     CDS             27436..27606
                     /locus_tag="VXD58_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675092.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27606..27857
                     /locus_tag="VXD58_000337"
                     /pseudo
     CDS             27606..27857
                     /locus_tag="VXD58_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27868..28208
                     /locus_tag="VXD58_000338"
                     /pseudo
     CDS             27868..28208
                     /locus_tag="VXD58_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28201..28581
                     /locus_tag="VXD58_000339"
     CDS             28201..28581
                     /locus_tag="VXD58_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0675093.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28594..28737
                     /locus_tag="VXD58_000340"
     CDS             28594..28737
                     /locus_tag="VXD58_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675094.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28830..29417
                     /locus_tag="VXD58_000341"
     CDS             28830..29417
                     /locus_tag="VXD58_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0675095.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            29619..29756
                     /locus_tag="VXD58_000342"
     CDS             29619..29756
                     /locus_tag="VXD58_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV0675096.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            29935..32544
                     /locus_tag="VXD58_000343"
     CDS             29935..32544
                     /locus_tag="VXD58_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV0675097.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKSMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            32577..>34336
                     /locus_tag="VXD58_000344"
     CDS             32577..>34336
                     /locus_tag="VXD58_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV0675098.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGISSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRAPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEK"
BASE COUNT        10450 a         6027 c         7591 g        10268 t
ORIGIN      
        1 aacaaaaggc ttctctataa aatcaaattt tccagatacc tactgaagct gatttatttc
       61 ttcatctttc ttctactacc tttgtcacta aatttgacag acacatattg ctgatacaac
      121 ctctctacct ataatctcac ggttaacctg aggctagttt catagtttgc tatttgattt
      181 tcgttgataa aaagaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac
      241 acatttattt cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa
      301 aagcaagggt agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga
      361 atagaataag agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag
      421 aagccagtga tcttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg
      481 aaggaaactt tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggtttg
      541 tcagcaaaga caaggtggcc ttcaatcaaa ctttctttga aacgttgttt agaaaagcca
      601 aagaaaagag aaacgcctac tatcaagatg atttgacaac tgcctttcgt ctctttaatc
      661 aagagggaga tggctttggg ggtctgacag tggaccttta tggcgactac gccgtctttt
      721 cttggtataa ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg
      781 ttttccctga ggttttaggg gcttatgaga aaatccgctt taagggtttt gactatgaat
      841 ctgcccatgt ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc
      901 tgtatcaagt ctttatgaat gatggcttga tgacaggaat tttcctagac cagcatgagg
      961 ttcgcggtag tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct
     1021 acacagcggc tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg
     1081 atctagccaa acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca
     1141 cagacgagca tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca
     1201 aagacttgac ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac
     1261 aaactttctc tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa
     1321 atccgggagg gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta
     1381 cagaacaaat tgataaaggc tttgcaggaa gaagttacca gattttaaac aaatatggtc
     1441 ttccagcaga ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta
     1501 gtatgaaggt tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc
     1561 tcaggctctg gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta
     1621 tctgcctaaa gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg
     1681 tgagttggtt ttcacgctac gaactcgctc cgaaggtgga gaaatcgacc tttctccaga
     1741 agagtatatc catctaatca aggaagttgc gcaattctat caaccagact atattgattt
     1801 tgagtactat agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt
     1861 gagttaccat aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac
     1921 gatcctaaat ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt
     1981 agacttgatg aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac
     2041 catttctatg ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag
     2101 ttggtctttt gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat
     2161 gaaaaaaatc agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg
     2221 ttgccaatcc tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga
     2281 cagctaccaa cggtgcttat gtggcttggg agattgaagc gagtgacttg gcagaaacag
     2341 tggccaatat tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc
     2401 aggtgattcc ttatttggat aagctgagcg atgaagcgcg cttgattggt gcggttaata
     2461 cggttgtcaa tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta
     2521 agtgcttgcc ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg
     2581 cggctaaatc aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg
     2641 ttcgttccgt ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag
     2701 gttttaaagt ggatttgtgt gctttagaga atgtttctga actgcaagca aggattgccg
     2761 agtcggattt gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc
     2821 ctgaaaacat agtcttacca gaaactctct tagttgcaga tatcatatac caaccctttg
     2881 aaacaccgtt tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa
     2941 tgttgctcta tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag
     3001 aagagatttg gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga
     3061 aaatcagaat cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg
     3121 cccaggctgg tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag
     3181 ataaccatgt agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt
     3241 ttcaggtagc tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc
     3301 agaaagtcta tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc
     3361 ttggtggtgg cgttgttggg gacctggctg gatttgtagc ctctacctat atgcggggta
     3421 ttcactttgt tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa
     3481 agacaggcgt taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg
     3541 gggttctgat tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga
     3601 tgggtgaagt tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg
     3661 aactgaatgg ttctgttgag agcattttgg aacatgcaga gaccttgatt gaacattctt
     3721 gtcaggtgaa gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc
     3781 tcaattttgg ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca
     3841 tgcatggaga ggctgttgcc atgggaatgg tacagatttc caaggttgct gaggaaaaag
     3901 gcctcatgcc agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc
     3961 ctgttgacta tgaaaattgg gaagttgaca agctttatca ggctcttact catgacaaga
     4021 aaacgcgtgg taacaccttg aaattggtct tggtgtcaga gcttggttca gcgatcattc
     4081 acccagtttc tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat
     4141 atttaactgc aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag
     4201 ctggacttcc attgacagct gaggatatca atgaggacct tagacgccgt cagggtggct
     4261 acggtcgtgg tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggcgttc
     4321 gccacgggaa gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga
     4381 aatggctgga catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa
     4441 ttactcatcc tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg
     4501 atttgcgaaa ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg
     4561 gtgcagtagc caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg
     4621 tctttggtgg caaggaaatc gatgttcctg aaaatctgac agtcgctgaa attaagcaaa
     4681 gagctgccca gtctgaagtt tctattgtta accaagaacg agaacaggaa atcaaggact
     4741 atattgacca aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg
     4801 gaggcgttcc agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat
     4861 tggctcaagc tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg
     4921 aggctggtta tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg
     4981 gttatactcg ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac
     5041 ccatcgttgt tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg
     5101 tggatatcga aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc
     5161 ttccagctgc aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc
     5221 tcgaaaaatt ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag
     5281 actatacaaa gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg
     5341 ggttgattgg agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt
     5401 taggttataa tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggc atgattgacc
     5461 gtgcaacgga tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc
     5521 caatcaaaca aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg
     5581 ttattatttc agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt
     5641 tggctggcaa gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag
     5701 gggctgcttc ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa
     5761 gcctgacaag tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc
     5821 gttttatcga gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc
     5881 ctcatatttt ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga
     5941 tggcaaggcg ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc
     6001 caggaatgtg gacctccatt ctcttgtcca atagcgagac cattctggat agaattgagg
     6061 atttcaagga acgtttggaa gtgattggtc aggccattag taagggagat gaagagcaaa
     6121 tttggaactt ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg
     6181 gtggtgtgga tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc
     6241 tgcggatttt ggaactgcta cgtgggactt ccttggttaa catccacatc aacgaggaaa
     6301 atcgtgagga tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa
     6361 gagctgagca tctcataaca gaaaataccg actatacagt cgtcatcaaa taaggagaag
     6421 aacatgtcaa atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca
     6481 gaatacaagg ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa
     6541 atctttacag aatatcttgc ctttcaagag gaaattcaaa aactagcaca tacaggtcaa
     6601 atgccagatg cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat
     6661 agcctcttgt cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt
     6721 gaaaaaattg tttttgaacc agtttcagaa ttgctgaaat aagaaaagaa cgatcctaaa
     6781 gtcaggaatt tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag
     6841 tatgaggtcc agtatgaaac taaaaacaaa cattcgccat ttacatggta gtatccgcgt
     6901 cccaggtgac aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga
     6961 gaccaaggtt tatgatattc tgcgaggtga agacgttctt tcgaccatgc aggtttttcg
     7021 tgaccttggt gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat
     7081 ggctggctta aaagcgccac aaaatgccct taatatggga aattctggca cctcgattcg
     7141 cctgatttca ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag
     7201 tctttccaaa cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat
     7261 ttcagggcaa actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag
     7321 acctattcat tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc
     7381 agccttacaa gctaaggggg agtcagttat tatcgaaaaa gagtgcaccc gtaatcatac
     7441 tgaagatatg ttgaaacaat ttggtggtca tttaagtgtg gacggtaaga aaatcacagt
     7501 ccaagggcca caaaaattga caggacagaa ggtggtcgta ccaggagata tttccagtgc
     7561 agccttttgg ttagtcgcag gtttgattgt tccaaattct cgtctagtgc tgcagaatgt
     7621 ggggatcaac gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt
     7681 ggaaataact gaaatcgatc cagtcgctaa atcttctacc ttgactgttg agtcttctga
     7741 cttgaaagga acagagattg gtggcgcttt gattccacga ttgattgatg aattgcccat
     7801 tattgccctg cttgcgactc aagcccaagg tgtaacagtt atcaaggatg ctgaggagct
     7861 caaggtcaag gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc
     7921 agatattact cctacggcag atgggatgat tatcaaaggg aaatcagctc ttcacggtgc
     7981 tagagtcaat acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctctt
     8041 ggttgcagat ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag
     8101 tttctttgat gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg
     8161 ctggaaaatc gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga
     8221 ttgagaagcg cctaggtatg tccattgcga attttttcgc tgaaaaggga gaagcagcct
     8281 ttcgtcaggt agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa
     8341 ctggaggagg agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata
     8401 acatctacct gaaagcagat tttgaaaccc tctaccaacg tatcgcagct gataaggaca
     8461 atcagcgacc gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa
     8521 gacaggcttg gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag
     8581 aggaaattat agaggaacta agatgaaaat tgcttatcta ggtcccaagg gatcattttc
     8641 acaccacgtt gtgcagatag cttttcctca tgaggaattg caggcttttg ctaacattac
     8701 agatgtcatc aaggcttatg agcaaggatt agtggactat tctgtggtgc cagttgaaaa
     8761 ttctattgag ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca
     8821 agcagtagca gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac
     8881 taagattgaa aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga
     8941 tgaacaatat ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt
     9001 tatttccgaa catccagacc agccctttgc agcagttgca cctagaagtt ctgctgaaga
     9061 atatggattg gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt
     9121 ctgggttcta ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag
     9181 tttggccttg acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt
     9241 tgcttggcga ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg
     9301 tgaatacttt ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca
     9361 aaaagaatta gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc
     9421 aatatcagac catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag
     9481 cagttgcgtt atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat
     9541 gagtttgcct atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg
     9601 gaacaagaaa gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa
     9661 tccaaatcac aagcactctc tttccctcca aaaaagaaaa gacggaagct ccgtcttaaa
     9721 cgaattttta tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg
     9781 tttttgcgtg gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa
     9841 gtagaagtct ttaatggtca ggacactaga gatggagtta atattttaat catgggtact
     9901 gatggtcgaa tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat
     9961 gtcgggggct cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat
    10021 atagatggtt atagtcaagt gattaacggt agaaaacaga cagataacaa gttaaacgta
    10081 gcctacgagt taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg
    10141 aaagataatt ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc
    10201 acagcgattg acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg
    10261 aatgggcgtc ccctaacaga agctacagtc ggagatgatt tatacgctac cgagactgag
    10321 tctccaaccc aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat
    10381 tatgctcgtt tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa
    10441 gttttaacag caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa
    10501 gctcttggaa aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca
    10561 aacggtttat ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa
    10621 ctgggtgact gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa
    10681 aacaaatatc aaaccaagct cgctcaaatg ggcttgagat aagataatac tcaatgaaaa
    10741 tcaaagtgca aactaggaag ctagccgcag gctgctaaaa acacagtttt gaggttgtag
    10801 ataagactga cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa
    10861 gagattttcg aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt
    10921 caggctttga ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa
    10981 acgatacgat agaaagaata atgaacaata tgactgattt aaaagcaatt caggctcgta
    11041 gtctggagat ggctgaatat tttgtggcct tttgtaaaga acatgatttg ctctgttatc
    11101 tctgtggcgg aggcgctatt ggtgcccttc gaaacaaggg atttattcct tgggacgacg
    11161 acctagactt ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt
    11221 atgcagatga acgttatttc ttgtcaaaga gtcacaagga ttttgttgat cgtaatcttt
    11281 ttattaccat tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt
    11341 tgccacatgg tctggccttg gatgttttgc ctttggatta ttatccgaaa aatccagctg
    11401 agcggaaaaa acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc
    11461 cagaaaagca tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa
    11521 aatctctctg ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg
    11581 ctgattgtga tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc
    11641 caatcacatc ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc
    11701 caatcggcta tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag
    11761 cagacaagca ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag
    11821 aatacaaggg agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct
    11881 cttggaggtg gtgcagagaa gattctatca accattgttt caaatctgga tccagaaaag
    11941 tatgatattg atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag
    12001 catgtacgca ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt
    12061 ttgtggagaa tgagaattta ttttccaaga ctggctcgtc gtttgcttgt aaaagatgat
    12121 tatgatgttg aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga
    12181 gaagtcaaga agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct
    12241 aaaagagaat cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa
    12301 aagaccagca attctatcaa ggaagtttat ccagattata cttctaaatt acagacaatc
    12361 tacaatggat atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag
    12421 attgctcctc aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt
    12481 gtagtggaag tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc
    12541 ggggctggtg atatggaaga ggaactgaaa aaacgagtca aagagtatgg gattgaggac
    12601 tatgtacatt tccttggtta tcaaaaaaat ccttatcagt atctatctca gacgaaagtt
    12661 cttttgtcta tgtctaaaca agaaggtttt cctggagtgt atgtggaggc cttgagtctg
    12721 ggactccctt ttatctctac gaacgttgga ggggctgagg aattatccca agaaggacga
    12781 tttggacaaa tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact
    12841 tctgcctcaa actttgatgt cgatgaggct agccaattca ttcaacaatt tacaattaca
    12901 aaacaaatcg aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag
    12961 tgtgattgtg ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca
    13021 gaagcagacc tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag
    13081 tggtcgcttg tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa
    13141 gaacgaagga ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct
    13201 gatttttatt gactcagatg attatatcca tccagaaatg attcagagct tatatgagca
    13261 attagttcaa gaagatgcgg atgtttcaag ctgtggtgtc atgaatgtct atgctaatga
    13321 tgaaagccca cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct
    13381 aaaggaatac ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag
    13441 acagattgca actgccctat cctttcctaa ggggttgatt tacgaagatg cctattacca
    13501 ttttgattta atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta
    13561 tttccataga ggggatagta ttacgaccaa accctatgca gagaaggatt tagcctatat
    13621 tgatatctac caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt
    13681 cgcttttttc agattggcct atgcccactt ctttattctg gataagatgt tgctagatga
    13741 tcagtataaa cagtttgaag cctattctca gattcatcgt tttttaaaag gccatgcctt
    13801 tgctatttct aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt
    13861 cataaatatt tccttatatc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt
    13921 acattagaac gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat
    13981 agtcagttat gccttgacgc tagtaagtgg aattgtgtat ctgtttaata ataataatgt
    14041 tagcttactt tctactttat tgttcttgtt ggttagtagc ttaattgctt gttggaatga
    14101 tatcaagtat tacttaatcc attttatttt ctatttaacc atttttgtat ttctggtatc
    14161 aagaccgacc attgattatt ttagggatgg tgctttggat acctatcatc caatagccta
    14221 tcgttttgcc tttatagttg tcatggtttc gattctgggc ttgaccacag gaggcattct
    14281 ggctcgttac ttcatagcta ggaagaaaat aaaagtagca aatataggaa attctctaaa
    14341 agaggtttat atcaagcggt tacgctttgt atcactagga gtttttcttc taacctatcc
    14401 tttctatttc attcggttat ttgaacggct cttgtatcgt ttgcagactt cctactatgc
    14461 ctactatgca aattttgaaa gtaaactgcc ttattttacc tacattttgt ctacctttac
    14521 ggtctatgca atgtgtatgt atctggcaac caagccaaag aaattgcagg ccacagcagt
    14581 gcttgtctcc tttattgcag ctaatactat tcatttggca attgggacac gaaatccctt
    14641 tattttaagt attttatttg cttttgttta ttactttatg cgggagcaaa ctgaaaaagg
    14701 aaaatggatt gggtttaaag aaaagttagc gatttttgta ggttctccta ttctcatgtt
    14761 agcgatggga gtactcaatt atgtacggga taatgtccaa gtttctcata caggtttctg
    14821 ggatatctta cttgacttta tctataaaca agggactagt tttggtgttc tggctcgagg
    14881 ttttctattt aacagtagcc tcccttaccg agatttccgt aattttactt ttggtcctgt
    14941 tcttgattat tttgcaaggg ggagtttggg agccattttc ggaggaaaag cctttgaaca
    15001 tacaaccaat agtgtggaac tagctattga tagtaatagt tatgcccaca atctatccta
    15061 tcttgtcttg aacaaggaat acttgaaagg gcatggtatc ggaagtagtt atatcatgga
    15121 gttgtatacc gactatggta tgattggagt ctttctgctt agtttcttac tcggcgtatt
    15181 atttatagcc atgctgcaag tagcctatcg ctcaaggaca atcctatttg ctttatccct
    15241 actcatcttg aataatctat tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa
    15301 tttatttaca atgcaattct ggggaattgt tcttgtgatt atatttgtag caaaaatgct
    15361 tacaaaggaa aactagtatc tactaaacaa aggagaaaaa aatcatgttt gaacattatt
    15421 cagtagctga tttgtttgca aatctttata aaaagcgaaa agcaaatatt ttagctctca
    15481 tcgctttatt tgctctcatt gctgtaccat ttacaattaa agcagttagg aataaaaaca
    15541 ctgtcaaaga tacaacaagt tattcaactt atcttatcta taaaatcact cctccaaaag
    15601 agtcggccaa aacgattttg aatcatcaaa ttggtggtta tagtgatttt tatgggaaat
    15661 tgattgatgg taatttgaat ggagcttatc ttttcaatga tgtagaaccc agtgagttga
    15721 aaaaaattgc cagtgaatta gacacgacag aaacaacctt gaaaaattca acgaatgact
    15781 attggtggaa aaaattgacc gtctactata tgattgacga tgcaggggtt ggtgtgaaaa
    15841 ttttgacatc aagtaaagat gccaatgact tgttagagaa aaaaattgat gggttgattg
    15901 agaaatttaa acatgcctat gcaaatgtga aaattgaaaa actggaaacc atcaactcta
    15961 aagaattgaa cgcaaatggt gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc
    16021 gtttagttgt tattggagtg gtttgtgtga ttttggttgt gatgggaaat gtgttagttt
    16081 atctctttaa tccaacaatc aatagagtag gtgatttttc tcagtatcaa attgattttg
    16141 taacagagat tacaacaatt gctaacctag cagatgtttt gtcatacaaa aatactggac
    16201 aggaattgac catcgttagc tcaaataaag ctatcctaga taaattgaaa cagagtcaag
    16261 aagctttaaa aggaatgcat tttgtagatt tacaggatgt atcatctctt ttggaaagag
    16321 atacagtcct tcttgttgaa gagtacggag tgactcgtta taagaaattt gagcaaagtc
    16381 ttcaaattct cagaaactta aatcgttcta tccttggtgt agcaaccttt aaattataag
    16441 tattctgatg tataaggtct tcaaaatcgg ctaagagctg attttgaagg ctttttctat
    16501 ctgtctgcag aaattttctt ttggagtctc ctatagaaag accctaggga aaatctatcc
    16561 gtatagagga gccttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa
    16621 agtatgaggt tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg
    16681 atgtctttag aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa
    16741 gaaaaaacag aagagttctt ttatgctatc ttggatgaac tagagatgcc tgatagcttt
    16801 ctggatacag gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt
    16861 gtgaccaagt caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat
    16921 atggaagaat tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc
    16981 gcagacaaaa ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag
    17041 gaagaagagc aagagcaggc tgaacaagaa gctgagagta agaaagaacc ttacatctac
    17101 tacatccttt cttttgctaa gttggctgac ttggtagttt ttgccaagac agtgactttt
    17161 gaaatggaaa cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg
    17221 gatattgaaa atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt
    17281 gcagacgata gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt
    17341 cacgatgcag tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag
    17401 attttaagaa gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta
    17461 ctataatagg aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag
    17521 ataaagatga cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct
    17581 ttcctcctaa aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt
    17641 gctaaggtat tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac
    17701 tttaactttg taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg
    17761 gaattgatgg ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga
    17821 aaacacgttg ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa
    17881 atcgctcaag ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca
    17941 attcttcgta ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta
    18001 gtcaacggaa cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat
    18061 gatgctcttg cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta
    18121 gatgggattg atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag
    18181 attgcctttg atgatgtagc ccacaaggga atccgcaata tcacaccaga agacgtagct
    18241 gtagctcaag agtttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca
    18301 ggtattgctg cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg
    18361 aatggcgtaa tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac
    18421 ggaccaggtg cgggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc
    18481 gttcgtcgtt tgaatgatgg taccattggc aaagacttca acgaatatag ccgtgacttg
    18541 gtcttggcaa atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctccagac
    18601 tcaaaaggtc aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag
    18661 caaatccttc aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag
    18721 attaataaag cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac
    18781 ctcttgaata ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag
    18841 tgccaatatc gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat
    18901 tgaggtctgc gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc
    18961 acatgatgag cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc
    19021 aagacgcttg aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc
    19081 ggttatcgtt gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca
    19141 tgaaaaattg cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat
    19201 ttatggtaat ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga
    19261 ctttccagag tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag
    19321 ccgtagtgtc ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc
    19381 caatgtagcg gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga
    19441 gggagacctc ttccatgagc gttatcgtca ggacttggta agagaatttg cgatgattaa
    19501 gcaagtgacc aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt
    19561 tatggttctg gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc
    19621 tttcaaagga aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa
    19681 ataaagaata gaagatagga tggggaaact cttaaccaga gggtttcata tcctttttgt
    19741 gaaaagaagt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg
    19801 ctcaaaacag tgttttgagg ttgcagatag aactgacgaa gtcagctcaa gacactgttt
    19861 tgaggttgca gatagaactg acgaagtcag taacatatat acggtaaggt gacgctaacg
    19921 tggtttgaag agattttcga agagtattag ttaaaaacgt gataaaggag aaataaagat
    19981 ggcagaaatt tatctagcag gtggttgttt ttggggccta gaggaatatt tttcacgcat
    20041 ttctggagtg ctagaaacca gtgttggcta cgctaatggt caagtcgaaa cgaccaatta
    20101 ccagttgatc aaggaaacag accatgcaga aacggtccaa gtgatttacg atgagaagga
    20161 agtgtcactc agagagattt tactttatta tttccgagtt atcgatcctc tatctatcaa
    20221 tcaacaaggg aatgaccgtg gccgccaata tcgaactggg atttattatc aggatgaagc
    20281 agatttgcca gctatctaca cagtggtgca ggagcaggaa cgcatgctgg gtcgaaagat
    20341 tgcagtagaa gtggagcaat tacgccacta cattctggct gaagactacc accaagacta
    20401 tctcaggaag aatccttcag gttactgtca tatcgatgtg accgatgctg ataagccatt
    20461 gattgatgca gcaaactatg aaaagcctag tcaagaggtg ttgaaggcca gtctatctga
    20521 agagtcttat cgtgtcacac aagaagctgc tacagaggct ccatttacca atgcctatga
    20581 ccaaaccttt gaagagggga tttatgtaga tattacgaca ggtgagccac tcttttttgc
    20641 caaggataag tttgcttcag gttgtggttg gccaagtttt agccgtccga tttccaaaga
    20701 gttgattcat tattacaagg atctgagcca tggaatggag cgaattgaag ttcgttctcg
    20761 ttcaggcagt gctcacttgg gtcatgtttt cacagatgga ccgcgggagt taggcggcct
    20821 ccgttactgt atcaattctg cttctttacg ctttgtggcc aaggatgaga tggaaaaagc
    20881 aggatatggc tatctattgc cttacttaaa caaataaaac agagagtggg gcttcccact
    20941 ttcttcattt ctagaatatg aatagaaggg atttatgaaa cacctattat cttacttcaa
    21001 accctacatc aaggaatcaa ttttagcccc cttgttcaag ctgttagaag ctgtttttga
    21061 gctcttggtt cccatggtga ttgctgggat tgttgaccaa tctttacctc agggagatca
    21121 aggtcatctc tggatgcaga ttggcctgct ccttatcttt gcagtaattg gcgttttagt
    21181 ggccttgata gctcaatttt actcagcaaa ggcagcagta ggttttgcta aggaattgac
    21241 aaacgatctt tatcgtcata ttctttcctt gccaaaggac agcagagacc gtctgacaac
    21301 ttctagtttg gttactcgct tgacttcgga tacctaccag attcagactg gtatcaatca
    21361 attcctgcgt ctctttttac gagcgcccat tatcgttttt ggtgccattt ttatggctta
    21421 tcgaatctca gctgagttga ctttctggtt cttagtcatg gttgccattt tgaccattgt
    21481 cattgtaggg ttatctcgat tggtcaatcc tctctacagt agtctcagaa agaaaacgga
    21541 ccaactggtt caggaaacgc gccagcaatt gcaagggatg cgggttattc gtgcttttgg
    21601 tcaagaaaaa cgagagttac agatttttca aacccttaac caagtttatg ctagattaca
    21661 agaaaagaca ggtttctggt ctagtttatt aacacctctg acctatctga ttgtcaatgg
    21721 aactcttctc gttattatct ggcaaggcta tatttcaatt caaggaggag tgctcagtca
    21781 aggtgctctc attgctctta tcaattacct cttacagatt ttggtggaat tggtcaagct
    21841 agccatgttg ataaattctc tcaaccagtc ctatatctca gccaagcgaa tcgaggaggt
    21901 ctttgcggaa tctcccgaaa acatccattc agaattagaa caaaagcaag ttaccagtgg
    21961 tcgggtttta caagtccaag aattgacttt tacctatcct gatgcggccc agccttctct
    22021 gagatacatt tcctttgata tgactcaagg acaaattcta ggtatcatcg ggggaacggg
    22081 ttctggtaag tcaagcttgg tgcaactctt acttggactt tatccaatag ataaggggag
    22141 cattgacctt tatcgagatg gatctagtcc tcgtaattta gagcagtggc ggtcttggat
    22201 tgcttatgtt ccccaaaagg tcgaactttt taagggaact attcgttcca acttgacttt
    22261 aggtttaaat caagaagtga ctgatcaaga actctggcaa gccttggaaa ttgctcaagc
    22321 taaggatttt gtcagtgata aggaaggact cttggatgct ctagttgaag caggagggcg
    22381 aaatttctca ggtggacaaa aacaaaggct gtctatcgca cgagcagtct tgcgccaggc
    22441 tccgtttctc atcctagatg atgcgacctc agcactggac accattaccg agtccaagct
    22501 cttgaaagct attagagaaa attttccaaa catgagctta attttgatct ctcaacgaac
    22561 ctcgacttta cagatggctg accagattct cctcttggaa aaaggtgagt tgctagctgt
    22621 tggcaagcac gatgacttga tgaaatccag tcaagtctat cgtgaaatca atgcatccca
    22681 acatggaaag gaggactaga atgaaacgac aaactgtaaa ccagacgctc aaacgtttag
    22741 ccgtagattt agcaaaccat cccttcctcc ttttcctagc ctttctagga actattgccc
    22801 aagttggctt atcaatttat ctacctattc tgattgggca ggtcattgac caagtcctag
    22861 tggctggttc atcaccagtt ttttggcaga tttttctcca gatgctcttg gtggtaatag
    22921 gaaatactct agtacaatgg gccaatcctc tcctctataa tcgtctaatc ttctcttata
    22981 ccagagattt acgagagcga atcatccata aactccatcg gttaccgatt gcttttgcgg
    23041 atcggcaagg caatggagag atggtcagtc gtgtaaccac ggacatcgaa cagttggcag
    23101 ctggcttgac catgattttt aaccaatttt tcattggtgt tttgatgatt ttggttagta
    23161 ttctagccat gctccaaatt catctcctca tgactctctt agtcttgctg ttgacgccac
    23221 tgtccatggt gatttcacgc tttattgcca agagatccta tcatctcttc cagaagcaaa
    23281 cagagacgag gggaattcag actcagttga ttgaagaatc gcttagccag cagactataa
    23341 tccagtcctt caatgctcaa acagaattta tccaaagatt gcgtgaggct catgacaact
    23401 actcaggcta ttctcagtca gccatctttt attcttcaac ggtcaatcct tcgactcgct
    23461 ttgtaaatgc actcatttat gcccttttag ctggagtagg agcttatcgt atcatgatgg
    23521 gttcagcctt gaccgtcggt cgtttagtga cttttttgaa ctatgttcag caatacacca
    23581 agccctttaa cgatatttct tcagtgctag ctgagttgca aagtgctctg gcttgcgtag
    23641 agcgtatcta tggagtctta gatagccctg aagtggctga aacaggtaag gaagtcttga
    23701 ccagtgacca agttaaggga gctatttcct ttaaacatgt ctcttttggc taccatcctg
    23761 aaaaaatttt gattaaggac ttgtctatcg atattccagc tggtagtaag atagccatcg
    23821 ttggtccgac aggtgctgga aaatcaactc ttatcaatct ccttatgcgt ttttatccca
    23881 ttagctcggg agatatcttg ctggatgggc aatccattta tgattataca cgagcatcat
    23941 tgagacagca gtttggtatg gtgcttcaag aaacctggct cacacaaggg accattcatg
    24001 ataatattgc ctttggcaat cctgaagcca gtcgagagca agtaattgct gctgccaaag
    24061 cagctaatgc agactttttc attcaacagt tgccacaggg atacgatacc aagttggaaa
    24121 atgctggaga atctctctct gtcggccaag ctcagctctt gaccatagcc cgagtctttc
    24181 tggctattcc aaagattctt atcttagacg aggcaacttc ttccattgat acacggacag
    24241 aagtgctggt acaggatacc tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg
    24301 ctcaccgttt gtcaaccatt caggatgcgg atttaattct tgtcttagta gatggtgata
    24361 ttgttgaata tggtaaccat caagaactca tggatagaaa gggtaagtat taccaaatgc
    24421 aaaaagctgc ggcttttagt tctgaataag ccattctctt ttgaaagttt atggacgaaa
    24481 aaagttgcct tcgagtgact tttttgttac aatagctaga aaaattgttc actgtaatac
    24541 tcaatgaaaa tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcacagcttt
    24601 gaggttgtag ataagactga cgaagtcagt tcaaaacact gttttgaggt tgcagataga
    24661 actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
    24721 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
    24781 aaaaaggagt ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaaaat
    24841 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
    24901 caagataagc cagcgttttt agaacaagct gagcagatta tagactatca gggagcttgg
    24961 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
    25021 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
    25081 tttactcccg acatgactac caatgcggtc aaagaagccc tgacagagat gctccagtca
    25141 ggaacaacaa cctttaacga tatgtataat cccaatggtg tggatatcca gcaaatttat
    25201 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
    25261 acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagacga aatcttaaaa
    25321 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
    25381 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
    25441 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
    25501 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
    25561 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
    25621 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
    25681 gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag
    25741 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
    25801 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
    25861 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
    25921 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
    25981 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
    26041 acagtagaac tttaaaagaa aaatcacgaa aaattttaaa aaaagtttgc aaaaatcttg
    26101 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
    26161 ggggagatcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
    26221 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
    26281 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
    26341 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
    26401 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
    26461 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
    26521 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
    26581 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
    26641 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
    26701 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
    26761 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacatag
    26821 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
    26881 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
    26941 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
    27001 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
    27061 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat cactggtctt
    27121 ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
    27181 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
    27241 cttaaataat agagtatcgc aatttgattg cggaatccct atttaccagt ctttgagagc
    27301 gataagcgat tgatagaaac tgataaattg atttggtttc ctgccaaaaa ttctttggca
    27361 ggatttttgt tttaataaac tttcgggggt agacactaga ttgtctaccc ctttttttgt
    27421 gtttcggagg taattatgca aaaggaagga cagtcatcac gtcaattcgt gaagagtcat
    27481 ctcatgacta tcatgggagt tgatattgta aaagcaactc agttagttga tgaaatggaa
    27541 caagtaggtt taatccgatt tgatgaattt ggaaatgttg gaattttagt cttggaggga
    27601 caataatgaa gcgtattacc gctaatcaat atcagacatc agagcgttat tataagctcc
    27661 ccaaactttt atttgagagt gaacggtata agaatatgaa actggaggtt aaggtggttt
    27721 actcagtttt aaaagatcgg ttggagttgt ctttgagcaa aggttggatt gatgaggatg
    27781 gggctattta tttgatttat tccaattcaa atttgatggc acttttaggc tgttcaaagt
    27841 caaaattact ctccatgtga gtttgaagtg acatttttag atgattacca taaaaaatat
    27901 aactacccac tattttacga atcctatctt caaaacgtta tggaattcct tgaaagtcaa
    27961 gacataaaga atggggttga tgcctttgta gatgatcatc aaaatctcgt ttttgtttta
    28021 tatggacaag gctatcgagc cgagggaaaa gagggaatac ttacaaccca agtaactgta
    28081 aaagcttatg atgaagacaa gaaaccgatt aacttcgcaa atttattaga ttccttaatt
    28141 tattagattc cttaatcgtg tcagaatatc aaatggaacc gaatctttgg gaggtctccc
    28201 atgattgatc tctatctaag taaaaataga caaagaaatc aacttctttt agatttcttc
    28261 caaaactatg gcatagaggt atcttgtcat tcagtttctg aaatgacaaa ggacaaatta
    28321 attgagatga tgagctattc ttcagattgt tttgagtttt tatctcccaa tttattacga
    28381 tttaagaacc gtgacaatct aagattaacg gatttcgttg agataatttt aaaaaatcct
    28441 gaactaacca tcagacttcc tcttgcggtt tcaaataagc gagtttatcc aagtctgaat
    28501 ttggaagagg ctagagctct attgccaaga gatacgaaac aattgattta catggcgcaa
    28561 acacattatt tatctaacta aaggagaagc aatatggctg aacgattttg ggagaacttg
    28621 tccattattt tggctgaaag aaatatcagc tggattgagt taaccagaaa aatgtttgcg
    28681 ggagagtttc actatccaag tgaattgaat cgtttgtatc aaaagattcg ccactagaag
    28741 atggaacaat gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt attagattta
    28801 gattatgaag atttatttcg gaggtaacta tgaaaagaat aattccagtt tatatattcc
    28861 aacaagtaaa tgtcctattg gtatctctat acttattgaa atttctttgt atcggtgagt
    28921 taactatact acagattctc tatggttcgt cactcatttc ttttttatgg atgtatggcc
    28981 aacgcaaaca agcgcataag gtcaatatga aatctaggat gaaatggctt ggtgttgaat
    29041 ttgttagcct actgattata agtctatgtt ttagtctgat tcatgctcaa ggaagcacga
    29101 atcaagcaaa cttaattggc cttcaacatc aaattccttg gttttcattt ttattgttct
    29161 taatcaatgc gagtatggtt gaagaatttc tgtatcgaga aattttatgg aatttggtca
    29221 gaaaattaga tatcagagtt gctttgacaa gtgttttatt tgccttagca catcatacag
    29281 gaaccattct agcttggtgt ttgtatgttt cacttgggat gtttttaggt atggtgcgct
    29341 ataaatctga cttatggggc agtatgggac tacatttggt gtggaaccta ctagtttata
    29401 gtttgctgct tttttaaaat aggtgacctg atttttaggt aacttctaaa actaacttcc
    29461 agttttggga gagagatgga agttactttg aaaagttacg gaaacattat tttctatgtg
    29521 acaacattgt ataattgctt gcattttact ggtggagaga gtataatgag ggtggataaa
    29581 gtacttaatc ccaaaaattt aaataggagg agctagctat ggcagaagaa gtattaaatc
    29641 ttcaattagt atctgttcaa gttgacgaaa cagatgaagt tgatggaatg cgatttagta
    29701 cattcagtac aaatcgttgc ggtaactgga gtgcttttag ttgggaaaat tgttaattca
    29761 tttaaattgt aaactttaga gattctctgg agttatggat gtcgttccaa tatgtgcacg
    29821 ttggaatgtt agtgcttata tggggagaat ccaaaggatt ctcccttttt atttaaataa
    29881 gtctgtctag ctatagatat attaagtttg ataaaatttc ttggagattt tgttatggat
    29941 tataatttca atttggagca tcctttcttt ttcactaata atgattatag tacagatact
    30001 agcataaagt accaagtaag tctgcctttt aattggcacg aagtaatgaa taatgatgag
    30061 tgggtttatc aataccctat tggaaaattt gttgaaagac aagggtggaa aatacacatt
    30121 agttcagagt ataacagttc acatgaattg ttacaagatg tagcaaaaat ttgccatgaa
    30181 atgcgaattc catttaaaca tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa
    30241 ttggttagtc gaggattttc tggtaaattt attacatgtt atccaaatca aaatgaatta
    30301 gagtcggttt tgcaaaggtt agaaagtgcc ttaaagcaat ataatgggcc atatatttta
    30361 agcgataaac gttgggatga agcaccaata tatctgaggt atggtgtgtt taggccgtca
    30421 agggatgatg aaaagaaggt tgctattgat gaattaattg ttggcgatga agttgttaaa
    30481 gatgaacgtt taccagtatt taaaattcca aaaggtatag tgccccctga ctttttgaat
    30541 aaatggcttg acaagaaaga taaaaaacag ggagactttc cctttattat agataatgca
    30601 attagatttt caaacagtgg tgggatatat aatgctaggc taaaagaaga tgggaaaaaa
    30661 ataattttaa aagaggctag accgtataca ggtctaggat ttgatggtac ttattcttca
    30721 gagaaattag cttcggagtg caaagcacta aaaattctaa atgaatggag tgagacgcct
    30781 aaaatatact ggcacggtaa gatctgggag cacaccttct taggaattga acatatgaag
    30841 ggcgttccac taaatagatg ggttaccaat aattttcctt tatatgaagt tgttgataag
    30901 accaaagatt atctattgag agtttcaaag atagtagaaa aactcatcga tttaacgaat
    30961 aagtttcatt ctgaaaatgt atatcatcaa gatttacatt taggaaatat cttggttaag
    31021 gatgaagatg agattagtat aatagactgg gaacaggctg tcttttcaaa tgatgaaaaa
    31081 gttgtacata aagttgcagc ccctggtttt agagcatggc gagaaacact tcccagtgag
    31141 atagattggt atggtattag acaaatagct cattatttat acatgccact agtaactaca
    31201 tcggatttga catacaacta tgtaagtcaa accaggatag aaggaaaaaa actatttgag
    31261 tctttgggtt atacaagaga acatatagat tatgtagaaa gtttattgtc atatctagat
    31321 tctaaatgtc cacagattga gaatatttcc aggaaaaaag ttttaaaatc catgcatgag
    31381 attagaacta tcgagagtga gcaagatata caagatttta ttataaaatt attgagaggg
    31441 tttacgctaa cttatggaca gtggcgtaaa gaatttcaat caagattctt cccagttcat
    31501 tattatggtc tgaattttaa tcaaggaatt gcgttttcag atttagctat cctttggagt
    31561 tatcaacaac tagctaagaa agtaaaaaat ttcaagtttg atgactatta tgagattcga
    31621 acacaggtta ttaatgaggc agtaaataat tttaagaaat cgtccctttc tggtcttttt
    31681 gatggtaaaa ttggtacaat ctggctcatt tatgaatttg gggagataga tcgggcagtt
    31741 gaacttttta caactcattt tatagagata tttgagaatt cacaaaataa aaatttatat
    31801 agtgggcagg ctggaatttt actggttgga ctgtattttc ttagtaaagg ggaaattgat
    31861 aataaactag gagaagaaat actaattagg cttagagaat atactttaaa ttatatagaa
    31921 aatccagaaa ctttttgtaa agttggcgct agtgatgttc agagtaatga tccttatgag
    31981 aattttggtg gattactata tggtcacgca ggggtagctt ggctcttcgg tgaagcttat
    32041 aaattaactg gtgaaagtat ctataaaaat ggtctagaat tggctgtaga taaagaatta
    32101 gttgcttata aagtagatag taataattca ttgcagtaca gccaaggtca tagattacta
    32161 ccatatctag caactggaag tgctggcctg cttttactta ttaatagaaa taaagaaatc
    32221 ttgtcaagca aatacttaaa atatttaact tctttagaga gggcaactga tgtagttttt
    32281 tgtgtattac caggtttatt taacggattt tgtggtcttg aagttgctaa taatatatat
    32341 agtgatatcg atgataactt ttcaggacaa aagaaattaa ttgaacaact ttatagatat
    32401 ttgtgtgtaa ttgaagaagg atttgttatt gcgggagata atggtttaaa gataacaaca
    32461 gatattgctt cgggatttgc gggtgttgca attggattgg tatcaataat ggataataaa
    32521 ttgacgattc ttcctcaaat ataatattta gataccatta ttaaggggtc atacttgtga
    32581 atcagagtaa tgaaatattt ttaaatacaa tacaggaaaa aacacactat aagaggtcta
    32641 tctcttcatc atttcaggaa ttaaaaaaat ggaattcttt gatatttagg agagggcgat
    32701 attggtacca atttgataat tggagtatac ccaatactgg tgtaataaag gaatggtatg
    32761 tcgaagataa tgactggatt cctaaggtta gttcttccag tttagacaat tcaaattcac
    32821 ttaaaattgt ctttgatgtg gaaaaatata ctttgccaga tgatagtatc ttacaggatg
    32881 ttaggatttc atctgatgag agttatatag ttttggttgt atcctcactg cgtagcacta
    32941 atttaattgg aataaaaaaa tatactatgg aagagttatt tatcattgaa gatatttcag
    33001 tagaaagttc gttctatttg ggtaaattcg gtgttatgta tactcgctcc aaagagtacg
    33061 ggaggccaag taagttattc tataaatctt ttgatagctt tacagaagag gaactgtttg
    33121 aagaaaacga atgctctttt cgattaaaaa tagtccacat tgatagcaat aattgttttg
    33181 taaagtcagt agactttcag aaaggcagga tttttctata cagtttcgac cggacgggtt
    33241 ttgttagaca ctcttacaca gaaacggtag ccccaacacc tagagatatc gctctattta
    33301 gtacagaaaa agcacagtac tttcttggga tctcttcaac tgaagaaaag aaagatcaac
    33361 taattctaag ggaaacttct tcgggagaca gagtttctat tactatacca tatcaagata
    33421 gggctagaag agttcactgt ataggacgtt atatcctttt agactgttca aacgcagcga
    33481 attccgtctt ttatcttgct acatttaaaa ataatagttt gagggagttg agtgttaata
    33541 agataacatt tgatgaaaag acaacacttt atgaaaattc attttctgat cgtgttcttc
    33601 tgtttttaga gaggagaact ttttacgaaa aaatacttag ttttgattta atagaaaata
    33661 cattaaaaac tgaatttgaa agacccatca taaaatcgaa taatactaaa tatttttcta
    33721 aggtgatttg gactaaaaaa gacagttatg atgtaagcat accgatttct ttattttgga
    33781 aaagtgaaga tacggatgag ctcccaagaa gaaaaaaatg tattctaagt gtctatgggg
    33841 cttatggtaa gaatgataat tctgatttag atgaaattat gttatctata atagatgcag
    33901 gttttattta tgctatagtt catgtaagag gtggaggcta tttgggtggt gaatggtatc
    33961 gctcaggaaa ggcgttaaac aaatggaatt ctatcagaga ttttattgag ggagtaaatt
    34021 atttaaggga aaatgatgta attgacagta agcgattagg tttaataact tctagtgcag
    34081 gtggaataat tgctggtgcg gtgttgaacg aggaaaaaaa tttattgcaa agcattctct
    34141 tattttcgcc atttataaat ccttatgata cacttcagaa tccaaatgat cctctttcta
    34201 agaccgaaat agcagagtgg ggagatatta gagcccctga agtaaaggct tatataaagt
    34261 catattcgcc tatgcaaaat attgaaaagg cacgagactc aaatactgtt atagttaata
    34321 ttttgggtga gaaaga
//
LOCUS       DAVLFB010000008        33767 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLFB010000008 DAVLFB010000000
VERSION     DAVLFB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.008"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            382..678
                     /locus_tag="VXD58_000345"
     CDS             382..678
                     /locus_tag="VXD58_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0675099.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            755..2785
                     /locus_tag="VXD58_000346"
     CDS             755..2785
                     /locus_tag="VXD58_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0675100.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVHLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYIP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2787..3428
                     /locus_tag="VXD58_000347"
     CDS             2787..3428
                     /locus_tag="VXD58_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675101.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3421..3714
                     /locus_tag="VXD58_000348"
     CDS             3421..3714
                     /locus_tag="VXD58_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675102.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            3965..4837
                     /gene="rsmA"
                     /locus_tag="VXD58_000349"
     CDS             3965..4837
                     /gene="rsmA"
                     /locus_tag="VXD58_000349"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV0675103.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4839..5717
                     /gene="rsgA"
                     /locus_tag="VXD58_000350"
     CDS             4839..5717
                     /gene="rsgA"
                     /locus_tag="VXD58_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV0675104.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5728..6384
                     /locus_tag="VXD58_000351"
     CDS             5728..6384
                     /locus_tag="VXD58_000351"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV0675105.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            6347..7009
                     /locus_tag="VXD58_000352"
     CDS             6347..7009
                     /locus_tag="VXD58_000352"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV0675106.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            7011..8267
                     /locus_tag="VXD58_000353"
     CDS             7011..8267
                     /locus_tag="VXD58_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV0675107.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            8257..9198
                     /locus_tag="VXD58_000354"
     CDS             8257..9198
                     /locus_tag="VXD58_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV0675108.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            9465..10292
                     /gene="purR"
                     /locus_tag="VXD58_000355"
     CDS             9465..10292
                     /gene="purR"
                     /locus_tag="VXD58_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV0675109.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10359..11609
                     /locus_tag="VXD58_000356"
     CDS             10359..11609
                     /locus_tag="VXD58_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV0675110.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11735..12529
                     /gene="pflA"
                     /locus_tag="VXD58_000357"
     CDS             11735..12529
                     /gene="pflA"
                     /locus_tag="VXD58_000357"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV0675111.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12604..13530)
                     /gene="yidC"
                     /locus_tag="VXD58_000358"
     CDS             complement(12604..13530)
                     /gene="yidC"
                     /locus_tag="VXD58_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0675112.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13608..13886)
                     /locus_tag="VXD58_000359"
     CDS             complement(13608..13886)
                     /locus_tag="VXD58_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV0675113.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13920..14660
                     /locus_tag="VXD58_000360"
     CDS             13920..14660
                     /locus_tag="VXD58_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV0675114.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            14716..15399
                     /locus_tag="VXD58_000361"
     CDS             14716..15399
                     /locus_tag="VXD58_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV0675115.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15511..16503
                     /locus_tag="VXD58_000362"
     CDS             15511..16503
                     /locus_tag="VXD58_000362"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV0675116.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16568..17107
                     /gene="rsmD"
                     /locus_tag="VXD58_000363"
     CDS             16568..17107
                     /gene="rsmD"
                     /locus_tag="VXD58_000363"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV0675117.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            17097..17585
                     /gene="coaD"
                     /locus_tag="VXD58_000364"
     CDS             17097..17585
                     /gene="coaD"
                     /locus_tag="VXD58_000364"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV0675118.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGRDIACYVPESILEEIRNEK
                     KD"
     gene            17569..18606
                     /locus_tag="VXD58_000365"
     CDS             17569..18606
                     /locus_tag="VXD58_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV0675119.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     INDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18664..18894
                     /locus_tag="VXD58_000366"
     CDS             18664..18894
                     /locus_tag="VXD58_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV0675120.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18918..20201
                     /gene="murA"
                     /locus_tag="VXD58_000367"
     CDS             18918..20201
                     /gene="murA"
                     /locus_tag="VXD58_000367"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0675121.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            20194..20382
                     /locus_tag="VXD58_000368"
     CDS             20194..20382
                     /locus_tag="VXD58_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0675122.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20421..21245
                     /gene="endA"
                     /locus_tag="VXD58_000369"
     CDS             20421..21245
                     /gene="endA"
                     /locus_tag="VXD58_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV0675123.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21474..22805
                     /locus_tag="VXD58_000370"
     CDS             21474..22805
                     /locus_tag="VXD58_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV0675124.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(23003..23073)
                     /locus_tag="VXD58_000371"
     tRNA            complement(23003..23073)
                     /locus_tag="VXD58_000371"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23039..23041),aa:Cys,seq:gca)
     gene            23489..27100
                     /gene="rpoB"
                     /locus_tag="VXD58_000372"
     CDS             23489..27100
                     /gene="rpoB"
                     /locus_tag="VXD58_000372"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV0675125.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            27133..30810
                     /gene="rpoC"
                     /locus_tag="VXD58_000373"
     CDS             27133..30810
                     /gene="rpoC"
                     /locus_tag="VXD58_000373"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV0675126.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKAFRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30921..31334
                     /gene="ndk"
                     /locus_tag="VXD58_000374"
     CDS             30921..31334
                     /gene="ndk"
                     /locus_tag="VXD58_000374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV0675127.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(32263..32895)
                     /locus_tag="VXD58_000375"
     CDS             complement(32263..32895)
                     /locus_tag="VXD58_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675128.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32897..33664)
                     /locus_tag="VXD58_000376"
     CDS             complement(32897..33664)
                     /locus_tag="VXD58_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0675129.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
BASE COUNT         9831 a         6582 c         7750 g         9604 t
ORIGIN      
        1 attcttattt agttactatg ttagaaaaac aatggcaaga agatttaaaa aaatatttat
       61 aaacatagtg aatcagtgac aaaaatgtcc ttatcctcgt atcaaaacag ttctaaagtt
      121 cgtctttaga actgtttttt tagatataag ctaaaaataa tatgaaatag ttagatttta
      181 aggacattga tgtccttgat agtgtgataa agatggagta taatgactat aaaagttcat
      241 ttcagggtgc tattttgaca atttagagaa aaacaaataa ttttagaata tcatttggta
      301 atataatagt atcaacgttg ataatggaaa gaggagcaag taactcaaca atcttgtaat
      361 caaaaataga ggagtatcaa tatgaaacaa acagttaaaa aattagctct tgtagcgagc
      421 attgcagcaa cattaggtgg aagtgtagca gttgcctctg cagccgttca atacccagaa
      481 ggtggtgttt ggacttatgg ttcaggtaac ggtggtgctt actcaaacta ctatcaccct
      541 tcaaaatacc atagttcaac agttgttagc agaaaaactg gttcatctga caagggatat
      601 gctggtgctg gagggacttc tcgtgcatgg attcgtactt cttggggaga gaaagttgca
      661 ttctactata atgtttagaa tggtatagcc ctattgttta gctttacttg gtcaccttct
      721 cactaagtga ccaagtaact ttttggagga aatgatgaaa cgtttattta ttttgatttc
      781 aatggtattg gtatcgcttt atatggtgat aacttccgtt gaccatcgag aagagatttt
      841 atttggtaac tatccttctg ttgatgtgac aggaatgatg ataaatcaac cagtagctag
      901 tcgcgaagag gtgacagagg ctttgagtca cttggcggta gagcataata gtctcatcgc
      961 ccgtcgaatc gttgagccaa atgaagctgg agaaacacgc tttacctatg ccacttatgg
     1021 tgagggaaag cttccagaag gtctgaccat ttcctccaag gagagtgcag aaacgagtga
     1081 tttattaggg tcttacttga ttgtgtcagg aagtttggat ggagtgagct tacagaccac
     1141 cttgaaagaa cttggctatc aaggctttgt ttcgaatgga gaagatccat tttcgatagt
     1201 cttactattg gcggccaccc ctatggtgct actgagttta gctatttttc tgctgacctt
     1261 tatgagtctg accctgattt atcggatcaa atcccttcgt caggcaggga ttcgcttaat
     1321 agctggtgag agcttgtttg gagttgctct cagaccagtg ttagaagatg tgagacagct
     1381 tatctgctca gtgctggtat ccagtctttt gggattgggg attctctggt atcaaggtgc
     1441 cttgtttatg gcaacggtac aactggtcat cattgctctt ctactttatg gattgaccct
     1501 ggcagggatt tctaccttac taagtgtcgt ccatctactt ggtttacagg aaaatagtct
     1561 ggtggatcta ttgaaaggga aactccctct caaacggatg atgacattga tgatggtggg
     1621 gcaactctta gctgtattgg tggtcggatc gagtgcgaca gctctcctac cccactaccg
     1681 tgaaatgcag gaaatggaga gagctagcaa taaatggagc cagtcctcag accgttaccg
     1741 tctatccttt ggttggtcta gtgcatttgc cgatgaagaa ggaacgcgta aggataatcg
     1801 tgagtggcag acatttactg aagaacggtt agccaataca gactcttttt atattatgag
     1861 caatgttgac aatttctcag atggagcaga agtggaccta gatggcaatc gtctcagtga
     1921 ctacataccg tcagggaatg ttatctatgt ctcgccgcgc tatctgatag aagaaaagat
     1981 taccgtttct tcagagttta tggacaagat gcaaaacttg tctgagggag agtttgggct
     2041 gatcttgcct gagagcttgc gagagcagtc tgcctactac caaggattgt ttacagatta
     2101 cctgcaaaac ttttcatctg aaagtgtaga agtgacgagt cagaaacact acttcccaca
     2161 ggtaaggcta gcttttacag aaacaggaca ggaacgtttc ctctataatg atgggtacaa
     2221 gacaacacgc cagtacctaa aagatccgat tattgtagtt ctaacgccgc aagcgactgg
     2281 aacaagacct gttgcaggga tgttgtgggg aactacggct aatagtgcct tgaaactaga
     2341 tcgatatgga gacagcatca cagctctaaa agagcaaggt ctgtatcaca aggtttctta
     2401 cttggtaaaa agccagctat tttttgccaa ggtactaaat gacaaacggg tggagtttta
     2461 ctctctcctt attgggacga ttttgaccct gtctacggct atcttgttat ttgattccat
     2521 gaatcttctc tattttgagc agttcagacg ggaacttatg attaaacgtc ttgctggtat
     2581 gacaatctat gagcttcatg gcaagtattt actggcgcaa ggaggagttc tcttgcttgg
     2641 cctagtccta tctagtattt tgacaagaga tggtttgatt agcgctctag ttgtagcttt
     2701 gtttacgctt aacgccctct tgattttagt aaggcaggat aaaaaagaag aagctggtag
     2761 catggcagta ttgaaaggaa aataagatga ttgatattca aggattggaa aagaaattta
     2821 atgaccgcgc gattttctct ggtttgaatc tcaagctgga gaagggcaag gtttatgcct
     2881 taatcggaaa gagtggaagc ggaaagacga cgctgctgaa tatcttggga aagctagaaa
     2941 agatagatgg tggaagggtt ctctatcagg ggaaagattt aaaaaccatt cctactcgtg
     3001 agtattttcg agaccagatg ggctatctct ttcaaaattt cggcctctta gaaaaccaat
     3061 caatcaaaga aaatttggat ttgggttttg ttggtcagaa aatctcaaaa gtagaacgtt
     3121 tggaaaggca agtgggggct ttagaaaaag ttaatctagg gtatttggat ttagaacaaa
     3181 aaatttatac tttatctggg ggagaggccc aacgagttgc ccttgctaag actattttga
     3241 aaaatccacc cttgattttg gcagatgaac caacagcagc tcttgatcct gaaaattcag
     3301 aggaggttat gaatctcttg gtggatttga aagatgaaaa tcgaattatc atcattgcga
     3361 cccataatcc cctagtctgg aataaggctg atgaaatcat tgatatgagg aaacttgctc
     3421 atgtgtgaaa aaatccgtat tcgcagggta tctgattatc ctagtgccag aggtggttta
     3481 gaagatatcc tcatcatgga aaatatgacc aatcatctcc ttttggttca aatccgagtg
     3541 catggctatt tgcttgattt tgctagtatt gaagggcaaa ggcaaaagca ttatcgtttg
     3601 aaaaatttac ctcagacggt tgaactgaca gtggatgatg tggaggagga tgtggatttg
     3661 accctacccg aaaatcgaag ttatcaagaa gctgattttt ttgatactca atgaaaatca
     3721 aagagcaaac taggaagcta gccgcaggct gtacttgagt acggcaaggt gaagctgacg
     3781 tggtttgaag agattttcga agagtatgaa cgtatgtttc gagagaagca ctaagtccct
     3841 ctttaaagat ttccaagact atctttcttc atgaggaaag atagtttttt ggtatgattt
     3901 tcattcccaa aatacaaggg gaatgtgtta caatagtagt aacagataat agaaaagaga
     3961 atagatgaga attgcagatt atagcgtgac caaggcagtg ttggagcgtc acggttttac
     4021 ctttaaaaag tcctttgggc aaaatttctt gacggatacc aatatccttc aaaaaattgt
     4081 ggatacggct gaaattgatg atcaggtcaa tgtcatcgaa atcgggccag gtattggtgc
     4141 cttgacagaa tttttggctg agcgtgcagc ccaagtcatg gcttttgaga ttgaccaccg
     4201 tttgatgcca attttggcag atactctgcg tgattttgat aatgtgaccg tagttaacga
     4261 agatattctc aaggttgatt tggcgcaaca tatccaaaat tttaaaaatc ctaacctgcc
     4321 aatcaaggta gtggctaatt tgccttacta catcacgacg cctattctca tgcacttgat
     4381 tgagagtggc attccttttt gtgagtttgt ggtcatgatg cagaaagaag tagcggaccg
     4441 catttcagcc cagcctaaca ccaaggctta cggtagcttg tctatcgccg tgcagtatta
     4501 catgacagcc aaggttgcct ttatcgtgcc tcgtacggtc tttgtgccag cgccaaatgt
     4561 ggattcagcc atcttgaaaa tggtgcgtcg tccagagcca gccgtagcag tagaagatga
     4621 gaactttttc tttaaggttt ccaaggctag ttttacccat cgccgcaaga ccttgtggaa
     4681 taacttgaca ggttactttg gtaagactga agaggtcaag gacaagctga ccaaggcttt
     4741 ggaccaggca ggcttgtcac caagtgtgcg tggggaagct ctcagcttgg cagaatttgc
     4801 cggtctagca gacgcactta aagggcaagg actctaagat gcagggacaa atcattaaag
     4861 ccttggcagg tttctactat gtggagagtg atggccaggt ttatcaaaca cgcgcgcgtg
     4921 ggaatttccg taaaaaaggc catacccctt atgttgggga ctgggtagat ttctctgccg
     4981 aggaaaattc agaaggctat atcctcaaaa ttcacgaacg gaaaaacagt ctggttcgtc
     5041 cgcctattgt caatatcgat caagctgtag taatcatgtc cgtcaaggaa cctgatttta
     5101 acagcaattt gctggatcgt ttcttggttc ttttggagca caagggcatc catcccattg
     5161 tctatatttc caaaatggat ttgttggaag ataggggaga actggatttt taccagcaga
     5221 cctatggtga catcggctat gactttgtga ccagtaaaga ggaactcctg tctttgttaa
     5281 caggcaaggt tacggtcttt atggggcaga caggtgttgg gaagtcaact cttctcaata
     5341 aaatcgcacc agacctcaat cttgaaacgg gagaaatttc agacagtcta ggtcgcggtc
     5401 gccataccac tcgagctgtt agtttttaca atctcaacgg gggtaaaatc gcagatacac
     5461 caggcttttc atctctggac tatgaagtat caagggctga agacctcaat caggcctttc
     5521 cagagattgc tactgttagc cgagattgta agttccgtac ttgtacccat acccatgagc
     5581 cgtcttgtgc cgtcaaacca gctgttgaag agggtgttat tgcaaccttc cgttttgaca
     5641 attacctgca attccttagt gaaattgaaa atcgtagaga aacctataaa aaagtcagca
     5701 aaaaaattcc aaaataagga gaaacctatg tctcaataca agattgctcc gtcaattctg
     5761 gcagcagatt atgccaactt tgaacgtgaa atcaaacgtc tagaagcaac tggggcagaa
     5821 tatgcccata tcgatatcat ggacagtcat tttgtaccgc aaatcagttt tggtgcaggt
     5881 gtggtcgaga gccttcgtcc tcatagtaag atggttttcg attgccactt gatggtgtca
     5941 aaccctgagc atcatctgga agattttgcg cgtgcaggtg cagacatcat cagtatccat
     6001 gtagaagcaa cgcctcatat tcatggcgcc ctccaaaaaa ttcgttcact cggagttaag
     6061 ccttcagtcg ttatcaatcc tggcacatca gttgaagcca tcaagcacgt ccttcatcta
     6121 gttgaccaag ttttagtcat gacggttaat ccaggttttg gtgggcaagc ctttctgcca
     6181 gaaaccatgg ataaggttcg tgagttggtt gctcttcgtg aggaaaaagg tttgaacttt
     6241 gaaatcgaag tggatggtgg gattgatgac caaactattg cccaagccaa agaagctggt
     6301 gcgaccgttt ttgtagcagg ttcctatgtc tttaagggag atgtcaatga gcgagtacaa
     6361 actctcagaa aacaactgga ctagggttgc cgtttttgca ggcggagacc gcggtcatta
     6421 tcggacagat tttgatgctt ttgttggggt ggatcgaggc tcgctctggg tcttggaaga
     6481 agacttgctt cttgctctag cagttgggga ttttgattct gtgaccgaag aagagcgaca
     6541 ggtgattcaa aaacgtgccc agcattttgt ccaagcccga ccagaaaaag atgataccga
     6601 tttggaattg gctctcttaa ccatctttga gaaaaatcct caggctcagg ttactatttt
     6661 cggtgccttg ggtggccgta ttgaccatat gttggccaat gtctttctgc ctagcaatcc
     6721 taagttggca ccctatatgc atcaaataga aattgaggat gggcaaaact tgattactta
     6781 ttgtccagaa gggaccagtc agctagaacc tcgttcagac tacgactatc tagcctttat
     6841 gccagttcgg gatagccagc tgactattct tggagccaag tatgagttga cagaggaaaa
     6901 ttttttcttt aaaaaagtgt acgcttctaa cgaatatata gatagggaag tgtcggtaac
     6961 ttgcccagat ggttatgtgg tcgtactgca tagcaaggac aggaggtagg atggaaagtt
     7021 tacttattct attattaatt gccaacctag ctggtctctt tctgatttgg caaaggcagg
     7081 ataggcagga gaaacactta agtaagagct tggaggatca ggcagatcat ttgtcagacc
     7141 agttggatta ccgctttgac caagccagac aagccagcca gttagaccaa aaagatttgg
     7201 aagtggttgt cagcgaccgt ttgcaagaag tgcggattga attgcaccaa ggtctgaccc
     7261 aagtccgtca agaaatgaca gataatctcc tccaaactag agacaagaca gaccaacgtc
     7321 tccaagcctt gcaggaatca aatgagcaac gtttggaaca aatgcgccag acggtcgagg
     7381 aaaaactaga aaagaccttg cagacacgct tacaggcttc ctttgagaca gtttctaaac
     7441 aactggagtc tgtcaatcgt ggccttggag aaatgcagac agttgcccgt gatgtcggag
     7501 ctcttaacaa ggttctctct ggaaccaaga cgcgagggat tctgggagaa ttgcaactgg
     7561 ggcaaattat tgaagacatc atgacacctg cccagtacga acgagaatac gcaacggttg
     7621 aaaactctag tgaacgagtg gagtatgcca tcaagttacc cggacaaggc gaccaagaat
     7681 acgtctatct gccaattgac tctaagtttc cactggcaga ttattaccgc ttggaagaag
     7741 cctatgagac aggtgacaag gatgagattg aacgctgtcg taagtcactc ctagcaagcg
     7801 tcaagcgctt tgccaaggat atcaagagca agtacatagc accgcctcgg acgaccaatt
     7861 ttggagtttt gtttgttccg acagaaggtc tttattcaga gattgtgcgc aatccggtct
     7921 tctttgatga tttgagacgg gaagaacaga ttatcgttgc aggaccaagt accctatcag
     7981 cccttcttaa ctccctatca gttggtttca agacccttaa tatccaaaag agtgccgacc
     8041 atatcagcaa gactcttgcc agtgtcaaga ccgagtttgg caagtttggt ggtattctgg
     8101 tcaaggcaca aaaacatctc caacatgcct ctggcaatat tgatgaatta ttaaaccgtc
     8161 gtaccatagc tatcgagcgg acgctccgtc acattgagtt gtcagaaggc gagcctgcgc
     8221 ttgatctact ccattttcaa gaaaatgagg aagaatatga agattagtca catgaaaaaa
     8281 gatgagctat ttgaaggctt ttacctaatc aaatcagctg acctgaggca aactcgagct
     8341 gggaaaaact acctagcctt taccttccaa gatgatagtg gcgagattga tgggaagctc
     8401 tgggatgccc aacctcataa cattgaggcc tttaccgcag gtaaggttgt ccacatgaaa
     8461 ggacgccgag aagtttataa caatacccct caagtcaatc aaattactct ccgcctgcct
     8521 caagctggtg aacccaatga cccagccgat ttcaaggtca agtcaccagt tgatgtcaag
     8581 gaaattcgtg actacatgtc gcaaatgatt ttcaaaattg aaaatcctgt ctggcaacgg
     8641 attgtccgaa atctctacac caagtatgat aaggaattct actcctatcc agctgccaag
     8701 accaaccacc atgcctttga aacgggcttg gcctatcata cggcgaccat ggtgcgtttg
     8761 gcagacgcta ttagcgaagt ttatcctcag ctcaataaga gcctgctcta tgcggggatt
     8821 atgttgcatg acttagctaa ggtcatcgag ttgacggggc cagaccagac agagtacaca
     8881 gtgcgaggta atcttcttgg acatatcgct ctcattgata gcgaaattac caagacagtt
     8941 atggaactcg gcatcgatga taccaaggaa gaagtcgttt tgcttcgtca tgtcatcctc
     9001 agtcaccacg gcttgcttga gtatggaagc ccagtccgtc cacgcattat ggaagcagag
     9061 attatccata tgattgacaa tctggatgca agcatgatga tgatgtcaac agctcttgct
     9121 ttggtggata aaggagagat gaccaataaa atcttcgcta tggataatcg ttccttctat
     9181 aaaccagatt tagattaata atttaagaaa aatgagcatt ttttaggata agaatgttcg
     9241 tttttttatg tgaatatggt ataataagta aaagacaaaa atgaatactc ttcgaaaatc
     9301 tcttcaaact agggtagtat cgccttgtcg tatgtatata tgcaggtata ttacagggtt
     9361 tgtcagttct attgacaatc tcaaaacagt gttttgaacc accagcgacc agctttctag
     9421 tttgcttttt gattttttga ataaaaatgg aataggaaat agaaatgaaa ttaagaagaa
     9481 gtgatcggat ggttgtcatt tccaactatt tgattaataa tccttataaa ctaactagtc
     9541 tcaatacttt tgctgaaaag tatgagtctg ctaaatcatc catctcagaa gatatcgtca
     9601 ttatcaaacg cgcctttgag gaaattgaaa tcggtcatat ccagacagtg actggggctg
     9661 gcggaggtgt catcttcaca ccgtctattt cgagtcagga tgctaaggaa atggttgaag
     9721 acttgcgtac caagttgtca gaaagtgacc gtatcttgcc aggtggttat atctatctgt
     9781 ctgatttgct tagcacacca gccatcttga aaaatattgg tcgtattatt gccaaaagct
     9841 ttatggacca aaaaattgac gcggttatga ccgtagcaac taagggtgtg ccacttgcaa
     9901 atgcagttgc caatgtcctc aatgtctctt ttgtcattgt gcgccgtgac ctgaaaatta
     9961 ccgaaggttc aactgttagc gtcaactatg tttcaggttc aagtggtgac cgtatcgaga
    10021 aaatgttcct ttcaaaacgt agtcttaagg caggtagccg tgtcttgatt gtggatgact
    10081 tcttgaaagg tggcggaacg gtcaacggta tgattagtct cttgcgcgag ttcgactcag
    10141 aactggcagg tgtagcggtc tttgcggaca atgcccaaga agaacgtgaa aagcagtttg
    10201 actacaagtc actcttgaag gtaactaata ttgatgtcaa gaaccaagcc atcgatgttg
    10261 aggttggcaa tatctttgac gaagataaat aagagataga actaaaggtt ggaacgattg
    10321 tcccagcctt tctttgcaaa cagaatagaa ggaagcttat gaaaacacca tttatcaata
    10381 gagaagagtt agaagcgatt gttgccgagt tcccgactcc ctttcacttg tatgatgaga
    10441 aggggattcg tgagaaggca agagccgtca accaagcttt ttcgtggaac aagggcttta
    10501 aggaatattt tgcagttaag gctactccaa ctccagctat tttgaaaatt ctccaagaag
    10561 aaggttgtgg tgtggactgc tctagttatg tagagctttt gatgagccat aaactggact
    10621 ttctgggttc tgagattatg ttctcttcca acaacacgcc agacaaggaa tacgcctatg
    10681 cacgtgaatt gggtgcgacc attaacttgg atgcctttga agatattgaa catctggaga
    10741 gagtagcagg cattccagaa atcatctctt gtcgttataa tcctggaggc gtttttgaac
    10801 tggggacaga cattatggac aatcctgggg aggctaagtt tggcatgacc aaggaccagc
    10861 tctttgaagc ctttgctatc ttgaaggaaa aaggagccaa gacttttggg attcactcct
    10921 tcctagcgtc caataccgtg acccatctct attatccaga gttggctcgt cagctctttg
    10981 aactggctgt tgaaatcaag gaaaagttgg gcatttggct agactttatc aatctttctg
    11041 gcggtattgg tgttaattat cgtccagacc aggagccgaa cgatatcgcc ttgattggtg
    11101 agggagttcg taaggtgtat gaagaagttc ttacgtcagc aggtcttggt caggtcaaga
    11161 ttttcaccga attgggtcgt tttatgctgg cacctcacgg tgctctagtc acaagagtga
    11221 ctcataagaa ggaaacctac cgtacctatc taggtgtgga tgcctcagca gtcaacctca
    11281 tgcgtccagc tatgtacgga gcttaccatc atattagcaa cgtgacccat ccagatggac
    11341 cagctgaagc ggtagatgtg gttggttcac tctgtgaaaa caatgataaa tttgcagtca
    11401 atcgcgaact gcctcataca gaaatcggtg atttgctggt aattcatgat acaggtgccc
    11461 acggattttc aatgggctac cagtataatg ccaaattacg ttctgcggaa atcctctata
    11521 ccgaagaagg taaagcccgt caaatccgcc gtgcagagcg ccctgaggac tattttgcaa
    11581 ccttatatgg cttcgatttt gaagaataat ctgataatag attgaaaatg aaattgaaaa
    11641 acagattgct ttctaaaaaa taggcaaaaa tcttgttttt ccttcaagtc gtgatataat
    11701 aaaactataa aacgttttca aggaaggtaa cgatatgtct gaagaaacaa ttgattatgg
    11761 acaagtgaca ggaatggtgc attcgacaga aagctttggg tcagtagatg ggcctggtat
    11821 tcgctttatt gtctttttgc agggctgtca catgcgttgc cagtattgcc acaacccaga
    11881 cacttgggct atggagtcca ataagtcacg tgaacggacg gtagatgatg tcttgacaga
    11941 ggccttgcgc taccgtggtt tctggggaaa taagggtggg attacagtca gtggaggaga
    12001 agctctcttg cagattgatt tcctgattgc tctcttcacc aaggctaagg aacaaggaat
    12061 ccactgtacc ttggatacct gtgctcttcc tttccgtaat aaaccacgtt accttgagaa
    12121 gtttgacaaa ctcatggctg tcactgactt ggttcttttg gatatcaagg aaatcaacga
    12181 agaacagcac aagattgtca ctagacaaac caataaaaat atcttggctt gtgcccagta
    12241 tctatcagat attggaaaac ctgtctggat tcgccacgtg ctagttccag gattgacaga
    12301 cagagatgat gacttgattg aacttggtaa gttcgtcaag accctcaaaa atgttgataa
    12361 gtttgaaatt ctaccttatc acaccatggg tgagttcaag tggcgtgaac ttggaattcc
    12421 atattccctc gaaggagtca aaccaccaac agcagatcgc gtcaagaacg ctaaaaaact
    12481 catggatacc gaaagttatc aagattatat gaaacgtgta catggataga aaagaagcct
    12541 gatggaaaca tcgggctttt gatttgcaaa aagacttagc aaatcagcta agcctttttc
    12601 ttcttatctc gaacgttgtt ttccagcgtt gcgatttttg tgttttttct tgcttgtgat
    12661 agcagttggt tgttcagggg taacgtcttt tcgtccactt ggtttagaga aagcacttgc
    12721 ttttggtggg ttcttggcta gttcttcacg gacttttttg cgaagttttg gacgaacgat
    12781 atagttgacg ataaactgtt ggagaatcat catgaaacca ccgacaaccc agtaaagtgt
    12841 gacactagct ggtgagaaga gggagaagac gacgatcatg agtgggctca tgtaaatcat
    12901 tttcttgatt tgttctcttt gcatttcatc ttctactccg tgaagtgaaa ggagcgattg
    12961 aagatagtaa aggacaccag cacaggcaac caaaatcata cttggagaac ctagaggaat
    13021 gcctaggtag cttgcttgag caaccccttc agtatgttgg gcagcaaagt agatagcaga
    13081 gaagaaaggc atttgaagga ggatagggaa acatcctaca ccgccaaaca tgctgatacc
    13141 gtgctctttt tgagcagcaa agagagcttg ttgggcttcg agtttttctt cttgagtagt
    13201 cgcttctttg agacgcgttt ggtgtggctc aaggacgtgc ttgagggcgt tcatcttttc
    13261 agagtgaagc gttgccttcc atgattggta gataccaagt ggcaagataa tcaagcgcac
    13321 gataatggtt acgatgatga tagcgacacc aaagcctaga cctttatcag tagcgaagta
    13381 cttgatagct tcagccatag gggctccgat ggtattccaa ataaatcctg ttggctgacc
    13441 tgtggtttta tcgacattga cacaaccagt caagacaagc aacatagccg ctcccatagc
    13501 tgagagtgca aaacgtttaa tagatttcac tgtttttatt ccttctttaa aaattatacc
    13561 tttctattct actgtttttt tacaaaatat acaatagttc tggagaccta atttgcgatt
    13621 ttaaagtcag agtagggagg aaagttgctt agtttgacat ctaagtagct tacttttgaa
    13681 aaaggtgtcg gtccttttcg gatttcttgg ataaattttg ccatgatagc agatgagtct
    13741 gcttgggcta agatttccac tgtgccatcg tcgttattcc atactcgacc tgtgatgcca
    13801 ccaatttcaa gtgccaagct gtaaacaccc caacgaaagc cgactccctg taccctacct
    13861 tgggcaatca ttctaacctt ttgcatacca aacccctttg attgtgttat aatatttcta
    13921 tgactattat aacctcaaaa gccaattctg tggtaaaaaa tgccaagaaa ttacgccaaa
    13981 aaaaataccg caagtctgcc tacttgattg aaggctggca cttgtttgaa gaagctgtcc
    14041 aagctggagt gacgattgag aaaatctttg ccctagaaaa ttatcgagat cagttagcta
    14101 ctttttcgca aactgtctgg gtgtcagagg atattttgct agatttggcg gattctcaga
    14161 ctccacaggg aattgttgcc gtggttcaaa aagaagaagt aggacaagct gatttgagtc
    14221 agggcaagtt cttgtttttg gaagatgtgc aagatcctgg taatgtagga actatcattc
    14281 gaactgcgga tgcagcaggt tttacaggag tgattgtttc agataagtcg gcagacatct
    14341 acagtctcaa aaccctacgt tccatgcaag gtagtcattt ccatctgccc atttaccgga
    14401 tgactagtca agcgcttctt gacgaaacta aaaaggtagc tatcccagtg ctagcaacaa
    14461 ccctatctaa agattctgtt gattacagag aactgccttc tatagaaaat tttgtactag
    14521 ttatgggaaa tgagggtcaa ggaattagtc cccttatggc tgaaagtgca gaccagttgg
    14581 tccatattag catgaagggg caggccgaga gtttgaatgt tgcggttgca gccggtattt
    14641 taatcttcca tttaagctaa ttttaacttt ttttgttata atcaaggaaa gatgttcaca
    14701 gaaaaggaga aatggatgaa tcacactatt atacatgacc gtgcaggtct caatcaattt
    14761 tacgctaagg tttatgcctt tgttggtctg ggaatcggac tatctgcttt ggtatcaggc
    14821 cttatgttga cggtctttca gtctcagttg gtttactttt tgatgcaggg gcgtctctgg
    14881 ttgaccattg ctacttttgc ggagttggct ttggttttcg ttgccagtag catggcttca
    14941 agaaatagtc cagctgctct tccagtattt ttactttact ccgttttaaa cggctttacc
    15001 cttagttttg tggtggcttt ctatactccg ggtacagttt tatctgcctt tgtatcgagc
    15061 gcccttctct tctttgtcat ggcggcagtt ggtattttta ccaagaaaga cttgagtggg
    15121 attggtcgag caatgatggc agctctcatt ggtttactga ttgctatggt agtcaatatt
    15181 ttcttggcta gtggcttctt tgattatatg attagcgtag ccatggtttt ggttttttca
    15241 gggttgattg cttgggataa ccaaaagatt cgcctcgcct atgaacaatc acaaggtcgt
    15301 gtagcgacag gctgggtggt gtcaatggct ctcagcatct atcttgattt tatcaatctt
    15361 ttcctaagta ttctacgtat ctttggtcgc aatgactaaa aggtctatga aatcagtgtt
    15421 ttgagtgaca ctgatttttt ttaaattttc tcttttcttt tcttggaaat aggtgtataa
    15481 tacgtttatt aaatttttga ggagttgtct atgaagaaaa gttttatcca tcaacaagaa
    15541 gaaatttcct ttgtcaaaaa cacttttacc cagtatttga aagataagct agaagttgtc
    15601 gaagttcaag gtcctatctt gagtaaggtc ggtgacggaa tgcaggacaa cctgtctggt
    15661 gtggaaaatc cagtatcggt caaggttctc caaatccctg atgctactta tgaagtggtg
    15721 cactcacttg ctaaatggaa acgccacacc ttggctcgtt ttggctttgg tgaaggagag
    15781 ggtctctttg tccacatgaa agcccttcgt ccagatgagg attccttgga tgcaacccac
    15841 tctgtttatg ttgaccagtg ggactgggag aaggttatcc caaatggtaa gcgtaacatc
    15901 gtttatctaa aagaaacagt tgagaagatt tataaggcta ttcgcctgac tgagctagct
    15961 gttgaagccc gctatgacat cgagtctatc ttgccaaaac aaatcacctt tatccataca
    16021 gaagaattgg tagaacgcta cccagacttg acaccgaaag aacgtgaaaa tgcgatttgt
    16081 aaagaatttg gagccgtctt tttgattggt atcggtggcg agttgccaga tggtaaaccg
    16141 cacgatggac gtgcaccaga ctatgatgac tggacaagcg agtctgagaa tggctacaag
    16201 ggtctaaatg gtgatattct tgtctggaat gagtctttag gtggagcctt tgagttgtct
    16261 tctatgggaa ttcgtgtaga tgaagaaact cttagacgtc aggtggaaat tacaggtgat
    16321 gaagaccgct tggaattgga atggcacaag tctttgttga atggtctatt cccattgaca
    16381 atcggtggag gaattggaca atctcgtatg gccatgttcc tacttcgcaa gagacacatc
    16441 ggagaagtgc aaacaagtgt ttggcctcaa gaagtccgcg atacttacga aaatattttg
    16501 tagagaatcg aaccgcaagg ttcggttttc tttctctttt tgtctataat ttggtataat
    16561 aaacagtatg aaaatcgtat caggaatcta tgggggacgt cccctcaaga cactagaagg
    16621 caagacgaca agacctactt cggataaggt taggggagcc atttttaaca tgattggtcc
    16681 ctactttgaa gtgggacgag tcttggacct ttatgcaggt agtggtggtt tatctatcga
    16741 agcagtatcg cgtggcatgt ccagtgctgt tttggtggag cgagaccgta aggctcagac
    16801 catcgtggct gaaaatatcc agatgaccaa ggaagttgga aaatttcaac tcctaaagat
    16861 ggatgcagaa agggcattgg aacaggtatc tggggaattt gacctcgttt tcttagaccc
    16921 tccctatgcc aaggaacaaa tcgtagcaga tattgaaaaa atggctgaga gagagctttt
    16981 ttctgaagat gttatggttg tgtgcgagac ggataaagcc gttgaacttc cagaagaaat
    17041 tgcctgtctg ggtatctgga aggaaaagat ttatggaatt agtaaggtga cagtctatgt
    17101 cagataagat tggcttattc acaggctcat ttgatccgat gacaaatggg catctggata
    17161 tcattgaacg ggcgagcaga ctttttgata agctttatgt gggtattttt tttaatcccc
    17221 acaaacaagg atttctccct cttgaaaatc gtaaacgggg gctagaaaag gctttgggac
    17281 atttggaaaa tgttgaagtc gtggcttctc atgatgaatt ggtggtcgat gttgcaaaaa
    17341 gattgggtgc tacttgtcta gtgcgtggtt tgaggaatgc gtcggatttg caatatgaag
    17401 ccagttttga ttactacaat catcagctgt cttctgatat agagactatt tatttacata
    17461 gtcgacctga acatctctat atcagttcat caggcgttag agagcttttg aagtttggtc
    17521 gggatattgc ctgctatgtt cccgagagta ttttggagga aataagaaat gaaaaaaaag
    17581 attagatggc ccttatacgt cattgcggcc ttgattgtga ctttcttggc atttgtagtg
    17641 cccttgcctt attatataga ggttccaggt ggttcggaag atattcgcca agtccttaaa
    17701 ataaatgaca cagaagataa ggaagctggt gcctatcaat tcgttacggt tggtgttcaa
    17761 catgccactt tagctcatat gatttatgct tggttgacac cttttacaga tattcgtagt
    17821 gctcaggaga ctacaggtgg ttcttccgat gttgaattta tgcgaatcaa tcaattctac
    17881 atgcaaacat cgcaaaatat ggccaagtat caaggactaa aaacagctgg taaggatatc
    17941 gaactcaagt actttggagt ttatgttttg aatgtgacgg ataattcaac ctttaaaggg
    18001 attctcaata tctctgatac agtcacagca gtcaatgatc agacctttga tagttccaaa
    18061 gacttgattg attacgtcag ttctcaaaaa ttaggggatt ccgtcaaggt cacctatgaa
    18121 gaggatgggc aaaccaagtc tgcagaagga aagattatca ccttggaaaa tggcaaaaat
    18181 ggaattggaa tcggcttgat tgaccgtaca gaggtaatca gcaatgtccc aattagcttt
    18241 tcaacagctg gtattggcgg tccaagtgct ggtctcatgt ttagtctagc tatctatact
    18301 caaatagctc acccagatct tcgtaatggt cgtattgttg ccggtacagg taccattgac
    18361 cgcgatggta atgtgggaga cattggaggt attgataaga aggttgtagc ttcggctagg
    18421 gcaggtgctg ctattttctt tgctcctgat aaccctgtta gcgaagaaga acaaaaggcg
    18481 catccggacg cgaaaaacaa ctaccaaaca gccctagaag cggctaaaac aatcaagacg
    18541 gatatgaaaa tcgtgcccgt taaaacccta caagatgcga ttgattactt gaaaaacaat
    18601 ccctaatttt tgagtgaagt ttccaaacta gcgcacaaac agagaagatg gtataatagt
    18661 caaatggttc aattattatt cactctaagt agtcatatgc tctttattta tgtgagtttt
    18721 taccttttaa aggatcttgt tagatgggag aaggttttaa aagtgacaga tgagaataca
    18781 agaaaagttc gtttattagt agcctttttt agcattgtca taggctacat cctgagttct
    18841 ttctttatta gcctgtatca tttgtggcaa gaagcgctta gaggattatt atgaaatcaa
    18901 gagtaaagga aacgagtatg gataaaattg tggttcaagg tggcgataat cgtctggtag
    18961 gaagcgtgac gatcgaggga gcaaaaaatg cagtcttacc cttgttggca gcgactattc
    19021 tagcaagtga aggaaagacc gtcttgcaga atgttccgat tttgtcggat gtctttatta
    19081 tgaatcaggt agttggtggt ttgaatgcca aggttgactt tgatgaggaa gctcatcttg
    19141 tcaaggtgga tgctactggc gacatcactg aggaagcccc ttacaagtat gtcagcaaga
    19201 tgcgcgcctc catcgttgta ttagggccaa tccttgcccg tgtgggtcat gccaaggtat
    19261 ccatgccagg tggttgtacg attggtagcc gtcctattga tcttcatttg aaaggtctgg
    19321 aagctatggg ggttaagatt agtcagacag ctggttacat cgaagccaag gcagaacgct
    19381 tgcatggcgc tcatatctat atggactttc caagtgttgg tgcaacgcag aacttgatga
    19441 tggcagcgac tctggctgat ggggtgacag tgattgagaa tgctgcgcgt gagcctgaga
    19501 ttgttgactt agccattctc cttaatgaaa tgggagccaa ggtcaaaggt gctggtacag
    19561 agactataac cattactggt gttgagaaac ttcatggtac gactcacaat gtagtccaag
    19621 accgtatcga agcaggaacc tttatggtag ctgctgccat gactggtggt gatgtcttga
    19681 ttcgagacgc tgtctgggag cacaaccgtc ccttgattgc caagttactt gaaatgggtg
    19741 ttgaagtaat tgaagaagac gaaggaattc gtgttcgttc tcaactagaa aatctaaaag
    19801 ctgttcatgt gaaaaccttg ccccacccag gatttccaac agatatgcag gctcaattta
    19861 cagccttgat gacagttgca aaaggcgaat caaccatggt ggagacagtt ttcgaaaatc
    19921 gtttccaaca cctagaagag atgcgccgca tgggcttgca ttctgagatt atccgtgata
    19981 cagctcgtat tgttggtgga cagcctttgc agggagcaga agttctttca actgaccttc
    20041 gtgccagtgc agccttgatt ttgacaggtt tggtagcaca gggagaaact gtggtcggta
    20101 aattggttca cttggataga ggttactacg gtttccatga gaagttggcg cagctaggtg
    20161 ctaagattca gcggattgag gcaagtgatg aagatgaata agaaatcaag ctacgtagtc
    20221 aagcgtttac ttttagtcat catagtactg attttaggta ctctggctct aggaatcggt
    20281 ttaatggtag gttatggaat cttgggcaag ggtcaagatc catgggctat cctgtctcca
    20341 gcaaaatggc aggaattgat tcataaattt acaggaaatt aggctggaga accagccttt
    20401 ttctaaagat aaggagaaat atgaacaaaa aaacaagaca gacactaatc ggactgctag
    20461 tgttattgct tttgtctaca gggagctatt atatcaagca gatgccgtcg gcacctaata
    20521 gtcccaaaac caatcttagt cagaaaaaac aagcgtctga agctcctagt caagcattgg
    20581 cagagagtgt cttaacagac gcagtcaaga gtcaaataaa ggggagtctg gagtggaatg
    20641 gctcaggtgc ttttatcgtc aatggtaata aaacaaatct agatgccaag gtttcaagta
    20701 agccctacgc tgacaataaa acaaagacag tgggcaagga aactgttcca accgtagcta
    20761 atgccctctt gtctaaggcc actcgtcagt acaagaatcg taaagaaact gggaatggtt
    20821 caacttcttg gactcctcca ggttggcatc aggtcaagaa tctaaagggc tcttataccc
    20881 atgcagtcga tagaggtcat ttgttaggct atgccttaat cggtggtttg gatggttttg
    20941 atgcctcaac aagcaatcct aaaaacattg ctgttcagac agcctgggca aatcaggcac
    21001 aagccgagta ttcgactggt caaaactact atgaaagcaa ggtgcgtaaa gccttggacc
    21061 aaaacaagcg tgtccgttac cgtgtaaccc tttactacgc ttcaaacgag gatttagttc
    21121 cctcagcttc acagattgaa gccaagtctt cggatggaga attggaattc aatgttctag
    21181 ttcccaatgt tcaaaaggga cttcaactgg attaccgaac tggagaagta actgtaactc
    21241 agtaaaagat aagcctaaac tcctatgtca cttatggatg taggagttct ttttactagt
    21301 ttaagcaggg ctagaacagg tactaagaaa aaatagcaac ttctaaaact aacttccagt
    21361 tttgggagag agatggaagt tactttgaga agttacggta agaaaaaata atatgtattg
    21421 gattaaattt tgtgatataa taaaggataa gatactattt taggagaaga actatggaag
    21481 acccaagtag tcagaatttg ttgctacaat ttgttttatt gtttatcttg acggtattaa
    21541 atgccttttt ctcagccaca gaaatggcca tggtttctct taatcgtgcc cgagttgaac
    21601 aaaaggcgga agaaggagac agacgctaca tccgcctgct gaaggtacta gaaaatccta
    21661 accacttttt atcaaccatt caggtaggaa tcaccctgat tacaatctta tcaggggcga
    21721 gtttaactaa tactctagga cgtgtgattg cctcttggct tgggaatggc gaaacagcac
    21781 aagccgtggc aacttttcta tccttggcat ttttgaccta tatttccatc gtttttgggg
    21841 aattatatcc taagagaatc gctcttaatc taaaggatgc cttggcaatt cgtacagcgc
    21901 cggttatcat tgggatcgga aaactagtca gtccttttgt ttggctttta gctgcttcga
    21961 ccaatttctt gagccatttg actcctatgt cattcgatga tgcggatgaa aagatgaccc
    22021 gtgatgaaat tgcctatatg ctgacaaata gtgaagaaac attggatgct gatgagattg
    22081 agatgctaca aggtgtcttt tcgctcgatg aactgatggc acgagaggtt atggttcctc
    22141 gaacggatgc ctttatggtg gatattcagg atgatagtca agccattatc caaagtattt
    22201 taaaacaaaa ttattctcgt atcccggttt atgatgggga taaggacaat gtaattggaa
    22261 tcattcacac caagagtctc cttaaggcag gctttgtgga cggttttgac aatattgttt
    22321 ggaagagaat tttgcaagat ccactttttg tacctgaaac tatttttgtg gatgacttgc
    22381 taaaagaact gcgaaatacc caaagacaaa tggcaatctt gctggatgaa tacggtggta
    22441 tggctggttt ggtgactttg gaagacctct tagaggaaat tgttggtgaa attgatgatg
    22501 aaacggacaa agcagcaata gatgttcatc agattggcga ggatacctac attgttcaag
    22561 gaactatgac cctcaatgat ttcaataatt actttgatgt tgaactggaa agtgatgacg
    22621 ttgataccat cgctggttat tatttgacag gagtgggaac tattccaacg actgagaaac
    22681 tcagctatga attggttagc caaaacaaac agtttatctt aaccaatgat aaagtgaaga
    22741 atggacgtgt taccaaggta aaagtccaaa tcacagaagt cgaaatagaa gaagaaacag
    22801 aataaactag aggcttttgt caccaggcgt gacgaagctt ttttcaattg atttcaaaag
    22861 ggaattatgc catggaaaga actctgccac aaactcgctc caaaagtttt taaagttata
    22921 cgtatttatt cgagggaaaa taaaaaaagc ccttcaaatt gggctttctg ttcatttgaa
    22981 acctgataaa tcaagttata gaaggcggta gacggatttg aaccgacgat caagcttttg
    23041 cagagccgtg ccttaccact tggctatacc gccttaacgt ttattattat accttgaaaa
    23101 tgattttcag tcaatagttt tttcagtttt ttgtaccata gcgtgattcg aatgtgatgc
    23161 caagttttct atgggaaaaa atttaccaaa aacctactaa aaccccttga aaaatctagc
    23221 taaatagggt ataatatggg taatcatttg tcgtaggttt tgtctgaaat attgtccaga
    23281 caaggctcac agcagttaaa tcttctgaaa aagtcagatt tagctgctct ttttgtgctt
    23341 tttttcaaga ttttgagcat ttgtaacaga ggcttaaaga ttctgaaaat tcgtcaagag
    23401 gacacggtga taaggggttt acaaccatat gacgattaga aaagcctgat tgacaaggct
    23461 tggaacttat ttacaaagga gaatcatctt ggcaggacat gacgttcaat acgggaaaca
    23521 tcgtacccgt cgtagttttt caagaatcaa agaagttctt gacttaccaa atttgattga
    23581 aattcaaact gactcattca aagctttcct agaccacggt cttaaggaag tgtttgaaga
    23641 tgtattgcca atttcaaact tcacagacac aatggagttg gaatttgttg gatatgaaat
    23701 caaggaacca aaatatacgc tagaagaagc tcgtatccac gatgctagct actcagcacc
    23761 aatttttgta accttccgtt tgatcaataa agaaacaagc gaaatcaaga cccaagaagt
    23821 tttctttggt gatttcccaa tcatgacaga aatgggtact ttcatcatca atggtggtga
    23881 acgtattatc gtttctcagt tggtccgctc accaggtgtt tactttaacg acaaagtaga
    23941 caaaaatggt aaggtgggct atggttcaac tgttatccct aaccgtggag cttggttgga
    24001 acttgaaagc gactcaaaag atatcaccta cactcgtatc gaccgtactc gtaagattcc
    24061 atttacaacc ttggttcgtg ctcttggttt ctcaggtgat gatgaaatct ttgatatctt
    24121 tggtgacagc gaattggttc gcaacactgt tgaaaaagat atccacaaga atccaatgga
    24181 ctctcgtaca gacgaagcct tgaaagaaat ttacgaacgc cttcgtccag gtgagcctaa
    24241 gacggctgaa agctcacgta gcttgcttgt agctcgcttc tttgacccac gtcgttatga
    24301 cttggcagca gttggtcgtt acaaaatcaa taaaaaactc aatgttaaaa cacgtttact
    24361 caaccaaacc attgcagagc cattggtaga ccctgaaact ggagaaatct tggtagaagc
    24421 tggtacgatt atgactcgta gcgtgattga aagcattgaa agccatttgg atggcgactt
    24481 gaacaagatt gtctacatcc caaacgatgc agccgttgtg actgagcctg ttgttcttca
    24541 aaaattcaag gttattgctc caactgatcc agatcgcgtc gtaacgatca ttggtaatgc
    24601 taacccagat gacaaggttc gtacggtgac tcctgcagat atccttgctg agatgagcta
    24661 cttcctcaac ttggctgaag gacttggccg tgtagatgat atcgaccacc ttggaaatcg
    24721 tcgtatccgt gcggttggtg aattgcttgc caaccaagta cgtttgggac tttctcgtat
    24781 ggaacgtaat gtccgtgaac gtatgtctgt tcaggacaat gaagtcttga caccacaaca
    24841 aattatcaat attcgtcctg taacagctgc agttaaagaa ttctttggtt catcacagtt
    24901 gtcacagttc atggaccaac acaacccgct ttctgagttg tctcacaaac gccgtttgtc
    24961 agccttagga cctggtggtt tgactcgtga ccgtgctgga tatgaagtgc gtgacgtgca
    25021 ctacactcac tatggtcgta tgtgtccaat cgagacacct gaaggaccta acatcggttt
    25081 gatcaataac ttgtcatctt acggacactt gaacaaatat ggttttgttc aaacaccata
    25141 ccgtaaggtt gaccgtgaaa caggtgttgt cacgaacgaa attgtttggt tgacagctga
    25201 tgaagaagat gaatatactg tagctcaggc taactctcgt ctgaatgaag atggaacctt
    25261 tgctgagaag attgtcatgg gacgtcacca aggggtcaac caagagtatc cagctaatat
    25321 tgttgactac atggacgttt caccaaaaca ggtagttgcc gttgcgacag catgtattcc
    25381 tttcttggaa aacgatgact ccaaccgtgc cctcatggga gccaatatgc aacgtcaggc
    25441 tgtgccattg attaatcctc aggcacctta cgttggtact ggtatggaat accaagcagc
    25501 ccacgattct ggtgcggctg tgattgctca gtatgatggt aaagttactt acgcagatgc
    25561 tgacaaggta gaagttcgtc gtgaagatgg ttcattggat gtttaccaca tccaaaaatt
    25621 ccgtcgttca aactcaggta ctgcttacaa ccaacgcact ctcgtaaaag ttggtgatgt
    25681 cgttgaaaaa ggcgatttca tcgctgacgg accttctatg gaaaatggag aaatggcgct
    25741 tggacaaaac ccaatcgttg cctacatgac ttgggaaggt tacaacttcg aggatgccgt
    25801 tatcatgagc gaacgcttgg tgaaggacga tgtctacaca tctgttcacc ttgaagaata
    25861 cgaatcagaa acgcgcgata caaagcttgg gcctgaagaa atcactcgcg aaattccaaa
    25921 cgttggtgaa gatgccctca aagaccttga cgaaatgggg attatccgta ttggtgctga
    25981 ggttaaagaa ggtgatattc ttgtaggtaa agtaacacct aagggtgaga aagatctttc
    26041 agctgaagaa cgtctcttgc acgctatctt tggagacaag tctcgtgaag tgcgtgatac
    26101 ttctcttcgt gtaccacacg gtgccgatgg tgtcgttcgt gatgttaaga tctttacacg
    26161 tgtaaatgga gatgagttgc aatcaggtgt taacatgttg gttcgtgttt acatcgctca
    26221 aaaacgtaag attaaggtcg gagataaaat ggccggacgt cacggaaaca aaggggttgt
    26281 ctctcgtatc gttcctgtag aagacatgcc ttaccttcca gacggaactc cagtcgacat
    26341 catgttgaac ccacttgggg taccatcacg tatgaatatc ggtcaggtta tggagcttca
    26401 ccttggtatg gcagctcgta ctcttggtat tcacatcgct acaccagtct ttgatggagc
    26461 aagttctgaa gatctttggt caactgttaa agaagcaggt atggatagcg atgccaaaac
    26521 gatcctttac gatggacgta ctggtgaacc gtttgataac cgtgtttctg tcggagtcat
    26581 gtacatgatc aaactccacc acatggttga tgataaattg cacgcgcgtt cagtcggacc
    26641 atactcaact gttacccaac aaccactcgg aggtaaggct cagtttggtg gacaacgttt
    26701 cggtgagatg gaggtttggg ctcttgaagc ctacggtgcg tcaaatgtcc ttcaagaaat
    26761 cttgacttac aagtcggacg atatcaacgg acgtttgaaa gcctatgaag ctattacaaa
    26821 aggcaaacca attccaaaac caggtgttcc agaatccttc cgagttcttg tcaaagaatt
    26881 gcaatctctt ggtcttgaca tgcgtgtcct agacgaagat gaccaagaag tggaacttcg
    26941 cgacttggat gaaggaatgg acgaagatgt catccacgta gatgaccttg aaaaagcccg
    27001 cgaaaaagca gcccaagagg ctaaagcagc ctttgaagct gaagaagctg agaaagcaac
    27061 aaaagcggaa gcaacagaag aagctgctga acaagaataa gcagttcact tagaatagaa
    27121 agggaagaaa tagtggttga tgtaaatcgt tttaaaagta tgcaaatcac cctagcttct
    27181 ccaagtaaag tccgttcatg gtcttatgga gaagtcaaaa aacctgaaac aatcaattat
    27241 cgtaccttga aaccagaacg tgaaggactc tttgatgaag tgatctttgg tcctacaaaa
    27301 gactgggaat gtgcttgtgg taagtacaaa cgcattcgtt acagaggaat tgtttgtgac
    27361 cgctgtgggg ttgaagtaac gcgtacgaaa gttcgtcgtg agcgtatggg acatatcgaa
    27421 ttgaaagctc ctgtatctca catctggtac ttcaagggga ttccaagccg tatgggcttg
    27481 acccttgata tgagccctcg tgccctcgag gaagttatct actttgcggc ttatgtggtg
    27541 attgatccta aggatacacc acttgagcac aagtctatca tgacagagcg cgaataccga
    27601 gagcgcttgc gcgaatatgg ttatggttca tttgttgcta agatgggtgc ggaagccatc
    27661 caagaccttt tgaagcaagt agatcttgaa aaagaaattg ctgaactcaa agaagaattg
    27721 aaaactgcta ctggacaaaa acgtgtcaaa gccatccgtc gtttggatgt tttggatgcc
    27781 ttttacaagt ctggaaacaa acctgaatgg atgattctta acatccttcc ggttatccca
    27841 ccagatcttc gtccaatgtt gcagttggat ggtggccgtt ttgcctcatc tgacttgaat
    27901 gacctttacc gccgtgttat caaccgtaac aaccgtttgg ctcgtttgct tgagttaaat
    27961 gcaccaggta tcatcgttca aaatgagaag cgtatgcttc aagaagcagt tgacgctttg
    28021 attgacaatg gtcgtcgtgg tcgtccaatc acaggaccag gtagtcgtcc attgaaatca
    28081 ttgagccaca tgcttaaagg taaacaagga cgcttccgtc aaaacttgct cggtaaacgt
    28141 gttgacttct caggacgttc cgttatcgcc gttggtccaa ctcttaagat gtaccaatgt
    28201 ggtgtgccac gtgaaatggc gattgaactc tttaaaccat ttgtcatgcg tgaaatcgtt
    28261 gcccgtgata tcgtgcaaaa cgtcaaagca gctaaacgct tggtggaacg cggagatgag
    28321 cgtatctggg atatccttga agaagtgatt aaagaacacc cagtgctttt gaaccgcgca
    28381 ccgacccttc accgtttggg tatccaagcc ttcgagccag tcttgattga tggtaaggct
    28441 tttcgcttgc acccacttgt ctgtgaagcc tacaatgctg actttgacgg ggaccaaatg
    28501 gccatccacg taccactttc agaagaagca caagcagaag ctcgtatcct catgctagct
    28561 gctgagcaca tcttgaaccc gaaagatggg aaaccggtag ttactccatc tcaggacatg
    28621 gttttgggta actactactt gaccatggaa gaagctggtc gcgaaggtga aggaatggtc
    28681 ttcaaagacc gtgacgaagc ggttatggct taccgcaatg gttatgttca cctccactca
    28741 cgtgttggta tcgcaacaga cagcctcaac aagccttgga cagaagagca aagacataag
    28801 gtcttgctta caacagttgg taaaattctc ttcaacgata tcatgccaga ggggctacca
    28861 tacttgcaag aaccaaacaa tgccaacttg acagaaggtg ttccagctaa atacttcttg
    28921 ccacttggtg gagctatcaa ggaagctatc agcaatcttg agctcaaccc tccattcaag
    28981 aagaaaaacc tcggaaatat catcgctgaa atcttcaaac gtttccgtac gacagaaact
    29041 tctgccctac ttgaccgcat gaagaacctc ggttaccacc actcaactct tgcaggattg
    29101 acagtgggta ttgccgatat cccagtcgtt gatgacaagg ctgaaatcat tgaagaatca
    29161 cacaaacgtg tcgaacaaat caccaaacaa ttccgtcgtg gtatgatcac agacgacgag
    29221 cgttacaatg ctgttacagc tgaatggcgt gctgcccgtg aaaaacttga gaaacgcttg
    29281 attgctaatc aagatcctaa gaacccaatc gttatgatga tggactctgg agcccgtggt
    29341 aacatctcaa acttctcaca gcttgccggt atgcgtggtc tgatggctgc tccgaatgga
    29401 cgtatcatgg aattgccaat cctttcaaac ttccgcgaag gtttgtcggt attggaaatg
    29461 ttcttctcaa ctcacggtgc tcgtaaaggt atgaccgata cggcccttaa gacagccgac
    29521 tcaggttact tgactcgtcg tttggtcgac gttgcccaag acgttatcat ccgtgaggac
    29581 gactgtggaa ctgaccgtgg tctcttgatc cgttctatcg cagaaggaaa agagatgatc
    29641 gagtctctcg aagagcgtct caacggtcgt tacactaaga aaactgttaa acatccagaa
    29701 actggtgcag tgattattgg tccaaatgaa ttgattacag aagacaaggc gcgtgaaatt
    29761 gtcaatgctg gtgtggaaga agtgactatc cgttctgtat ttacatgtaa cactcgtcac
    29821 ggtgtctgcc gtcactgtta cggtatcaac ttggcgactg gtgatgcggt tgaagttggt
    29881 gaagcagttg gtacaatcgc tgcccaatct atcggggaac ctggtacaca gcttacaatg
    29941 cgtaccttcc acacaggtgg ggttgcctca aataccgata tcactcaggg tcttcctcgt
    30001 gtccaagaaa tctttgaagc ccgcaatcct aaaggggaag cggttattac agaggttaaa
    30061 ggacaagtta ctgctatcga agaagatgca tcaactcgta ccaagaaagt ctttgttaag
    30121 ggtgaaactg gcgaaggtga atatgtcgtt ccatttacag ctcgtatgcg tgtcgaagtt
    30181 gggggccaag tagcgcgtgg tgctgctctg acagaaggtt ctatccaacc aaaacgtctc
    30241 cttgcagttc gtgatgtctt gtcagttgaa acgtaccttc tcggtgaagt acaaaaagtt
    30301 taccgtagcc aaggggtaga aatcggtgac aaacacatcg aggtaatggt tcgtcaaatg
    30361 atccgtaaag tccgtgtcat ggatccaggt gatacagatc ttctcatggg taccctcatg
    30421 gatatcaatg actttacaga tgccaacaaa gatgtcctta tcgcaggtgg agttccagcg
    30481 acaggtcgcc cagtccttat gggaattacc aaagcctcac ttgaaacaaa cagtttcttg
    30541 tcagcggctt ccttccagga aacaactcgt gtccttactg acgcagctat ccgtggtaag
    30601 aaagaccatc tccttggact taaagaaaat gttatcatcg gtaagatcat cccagctggt
    30661 actggtatgg cccgttaccg taaccttgaa ccacatgctg tcaacgaaga agaatacctt
    30721 aatcctccag tagaggaaga aggaaatgaa gaaacaacag aagtagttgt ggatactgcc
    30781 gttgaaactg tggaagaaac agtagaataa gagagaatga gagaacccga gggttctctc
    30841 attctctctt ctgaaaactt tctccatttt tccacgaaat gtggtaaaat aaaagaaaga
    30901 agctgacaaa ggagacgggt atggaacaaa cattctttat catcaaacca gatggtgtaa
    30961 aaagagggct agtgggtgaa gtgttaaagc gcatcgaaca acgtggattt acaatcgaaa
    31021 aattggagtt tcgttcacag gtttcagaag agttgattga ccagcactat caggacttgg
    31081 ttggtcagag tttttaccca ccgattcgtg aattcatgac ttcaggtcca gttcttgtgg
    31141 gtgtcatttc tggtcccaaa gtaatcgaaa cttggcggac catgatgggt gcaactcgtc
    31201 cagaagaagc tttaccaggc actattcgag gtgattttgc aaaagctgct ggagaaaatg
    31261 agattatcca aaatgttgta catggttcag attcagaaga gtcagctaag cgagaaattg
    31321 ctctttggtt ttaagagtgg attggctcaa tcaattggtt aaaagctcat ttgaatagaa
    31381 agtatagtca attagtttaa gacatgacgc atgatatcaa actttttagt ttttgatatg
    31441 gtgcgttttt tgattaagtg actattagtt tgtctcgctc cgttaggtgc gagacaaaaa
    31501 aaacacccgc tatgcgggtg tgcgtcgaag gttataccaa aaaaactcca aacgcgatac
    31561 aataaaggtg ttcaatccaa ttgtaaagcg aaaggagaaa aatatggcac aaaaggttct
    31621 tttatcctgt tcttgtttca attgactata tttctatact ttttcttcct ttctctgtga
    31681 aaatcgcaag tttttcccac cttttctcaa tactgaaaca acatctttcc agaacttcaa
    31741 taatttcttt ttgtgttaaa tcattattta actaaatctt tggaggcaat acgttttcaa
    31801 agctactttt ttaccatagg ttttgagcct tcaacacaga ttactaagcc aaaaatgctc
    31861 taaatttttg aaattctaac aaattccact caactttatc atcatctttg actttcacaa
    31921 tatcttcctc aaaataacat ggaataaaga tagttttctt ttaaaacggt tgtaggagga
    31981 aaatctctcc tacactatta ttgattctga gagaaaattg caaaatatca tctaccaaac
    32041 tttcaattat taagattttt tgcagtttat ctgaattaga atcgcaaggc ttctcaataa
    32101 tataattgtc gaattttttc attctaactt cctctccatt atttcttcct ttatcaagaa
    32161 aaagttacct caatattaat aaagtacaat tctaaattaa ctccaattct tgattcatga
    32221 taatatttca gtaactcatc gtgcaattaa aaacaaggat ttttatttca gatgatccat
    32281 cgtgaacact tcatcagcca tctcccaaat tgccggatta tgtgttgcga taatgattag
    32341 cctattatca tctcgaagag atagcaaaat ctccataatc aactgagagg ttgctgggtc
    32401 tattgaagct gttggctcat ctgccagaat aaagggtgga ttctttaaga taatttttgc
    32461 caaggcaacc cgttgcgatt ctccgcccga taactcaaag atgcgcttat ctaggtcaag
    32521 ataaaccagg ccgacctgtt ctaaagcctg cttctgcctc aaccgctgtt ccgaccgact
    32581 caacttttga ccaatgagac ctagcttaag gttttcttca atactttggt tttcaattaa
    32641 gccaaagttc tggaagaggt agcccaattc gtgacggaaa aaatcacttg atttataatt
    32701 ggccaagtct ttacctcggt aaaaaatcgt cccatcataa ggttctaatt tcccaatcat
    32761 gttcatcaag gttgtttttc cgctaccact tgaaccaatt aaggcataga cttttccagc
    32821 ctcaaatgtc attgaaagat tcgaaaataa ctctcgttct ccaaaagatt tactcacctg
    32881 tttaagttca atcatattaa cctcccttca aaacaagcat ggacatcttg ttttctttct
    32941 gcatttggac atgtaactgt aaaagagata gaccagtaaa gagtaacaag actaagaaag
    33001 caactcctat ctctacctga agaaatacac tcgcaacaaa tcccagtaaa aacacaccca
    33061 gttgagcaaa gagataagtg cgatggattt ctaagaacct gagacctgca atgcgtttga
    33121 taaaaatggc acgtctaaat tcttcaaagt agagcctatt catagtgtta aacaacaaga
    33181 ttgaagttgc aatcccaagc acagctcctg ctagcattac ccaacgttcc ctctggatat
    33241 tatccaataa tgtgatgtag ttgtggtaac ctgtttgcat ttctgagacc caattttcaa
    33301 tgccttgtct ctggataagc tcctgggcat cagacaattg attaaagaga acgtagttct
    33361 gtactgcgtc tatccaaaac aaaatggact gtggaccagt tgattggggt gttataacaa
    33421 tgatgattgg atctttcaaa aactgctggt aagaaatagg ggtcgtatta tacacaaaac
    33481 gatcctgacc tgattctaaa taacctaccg tagcagtcat ttgctgtcgt tcatcttgac
    33541 tagatatgcg actggttaag tcgtcttcaa aaacagattt ataatgttct tcctctgaac
    33601 ggaggtgttc aggcagcaat aagacaaact cccctacatc aaggtgattc atcttttgac
    33661 tcattttagt tgaactgtag gacttacttt ttcagagggc tccaaatttt attttcattt
    33721 acttttttta gcccataaaa tatttaccat acttataaaa agtagta
//
LOCUS       DAVLFB010000009        33654 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLFB010000009 DAVLFB010000000
VERSION     DAVLFB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.009"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            65..637
                     /locus_tag="VXD58_000377"
     CDS             65..637
                     /locus_tag="VXD58_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675130.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(1681..3939)
                     /locus_tag="VXD58_000378"
     CDS             complement(1681..3939)
                     /locus_tag="VXD58_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEV0675131.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(3965..5482)
                     /gene="malQ"
                     /locus_tag="VXD58_000379"
     CDS             complement(3965..5482)
                     /gene="malQ"
                     /locus_tag="VXD58_000379"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV0675132.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            6032..7303
                     /locus_tag="VXD58_000380"
     CDS             6032..7303
                     /locus_tag="VXD58_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0675133.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKNAKTAANDAVTLIKETIKQKFGE"
     gene            7414..8706
                     /locus_tag="VXD58_000381"
     CDS             7414..8706
                     /locus_tag="VXD58_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0675134.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            8708..9550
                     /locus_tag="VXD58_000382"
     CDS             8708..9550
                     /locus_tag="VXD58_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0675135.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            9803..10603
                     /locus_tag="VXD58_000383"
     CDS             9803..10603
                     /locus_tag="VXD58_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV0675136.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            10613..11599
                     /locus_tag="VXD58_000384"
     CDS             10613..11599
                     /locus_tag="VXD58_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0675137.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            11735..11815
                     /locus_tag="VXD58_000385"
                     /pseudo
     CDS             11735..11815
                     /locus_tag="VXD58_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(11980..12921)
                     /locus_tag="VXD58_000386"
     CDS             complement(11980..12921)
                     /locus_tag="VXD58_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0675138.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(12899..14662)
                     /gene="aspS"
                     /locus_tag="VXD58_000387"
     CDS             complement(12899..14662)
                     /gene="aspS"
                     /locus_tag="VXD58_000387"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV0675139.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(14943..15023)
                     /locus_tag="VXD58_000388"
                     /pseudo
     CDS             complement(14943..15023)
                     /locus_tag="VXD58_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(15059..15289)
                     /locus_tag="VXD58_000389"
     CDS             complement(15059..15289)
                     /locus_tag="VXD58_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675140.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(15307..15948)
                     /locus_tag="VXD58_000390"
     CDS             complement(15307..15948)
                     /locus_tag="VXD58_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0675141.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVAFAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(15960..16682)
                     /locus_tag="VXD58_000391"
     CDS             complement(15960..16682)
                     /locus_tag="VXD58_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV0675142.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            complement(16685..16882)
                     /locus_tag="VXD58_000392"
     CDS             complement(16685..16882)
                     /locus_tag="VXD58_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675143.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNLYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(16882..17088)
                     /locus_tag="VXD58_000393"
     CDS             complement(16882..17088)
                     /locus_tag="VXD58_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675144.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(17098..17412)
                     /locus_tag="VXD58_000394"
     CDS             complement(17098..17412)
                     /locus_tag="VXD58_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675145.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     FQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(17667..18956)
                     /locus_tag="VXD58_000395"
     CDS             complement(17667..18956)
                     /locus_tag="VXD58_000395"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV0675146.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETISMAAYFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(19034..20281)
                     /locus_tag="VXD58_000396"
     CDS             complement(19034..20281)
                     /locus_tag="VXD58_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV0675147.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            20498..21844
                     /locus_tag="VXD58_000397"
     CDS             20498..21844
                     /locus_tag="VXD58_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV0675148.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
     gene            complement(22180..23031)
                     /gene="rgg"
                     /locus_tag="VXD58_000398"
     CDS             complement(22180..23031)
                     /gene="rgg"
                     /locus_tag="VXD58_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV0675149.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            23155..23496
                     /locus_tag="VXD58_000399"
     CDS             23155..23496
                     /locus_tag="VXD58_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0675150.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            23554..23634
                     /locus_tag="VXD58_000400"
                     /pseudo
     CDS             23554..23634
                     /locus_tag="VXD58_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(23750..25453)
                     /gene="ilvD"
                     /locus_tag="VXD58_000401"
     CDS             complement(23750..25453)
                     /gene="ilvD"
                     /locus_tag="VXD58_000401"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV0675151.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(25686..26618)
                     /locus_tag="VXD58_000402"
     CDS             complement(25686..26618)
                     /locus_tag="VXD58_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV0675152.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(26615..27472)
                     /locus_tag="VXD58_000403"
     CDS             complement(26615..27472)
                     /locus_tag="VXD58_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0675153.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(27476..28822)
                     /locus_tag="VXD58_000404"
     CDS             complement(27476..28822)
                     /locus_tag="VXD58_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0675154.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(28835..29119)
                     /locus_tag="VXD58_000405"
     CDS             complement(28835..29119)
                     /locus_tag="VXD58_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0675155.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(29123..31153)
                     /locus_tag="VXD58_000406"
     CDS             complement(29123..31153)
                     /locus_tag="VXD58_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV0675156.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEYYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            31344..32351
                     /locus_tag="VXD58_000407"
     CDS             31344..32351
                     /locus_tag="VXD58_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0675157.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            32354..32554
                     /locus_tag="VXD58_000408"
     CDS             32354..32554
                     /locus_tag="VXD58_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0675158.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            32706..32888
                     /gene="rpmF"
                     /locus_tag="VXD58_000409"
     CDS             32706..32888
                     /gene="rpmF"
                     /locus_tag="VXD58_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV0675159.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            32904..33053
                     /gene="rpmG"
                     /locus_tag="VXD58_000410"
     CDS             32904..33053
                     /gene="rpmG"
                     /locus_tag="VXD58_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0675160.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(33545..>33654)
                     /locus_tag="VXD58_000411"
     CDS             complement(33545..>33654)
                     /locus_tag="VXD58_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0675161.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT        10464 a         7156 c         6071 g         9963 t
ORIGIN      
        1 ttttgactat aataacagta tatgataaaa tgtaagcaga tacaaataaa aaggagaaaa
       61 gctcatgaac aaattaatga aatttatttc ggtttttttg acgtcaattg tgttaattgt
      121 atcagcgatt ccaagtgttt cagctgtata cgcttctgaa caagtatcac aaattgaaac
      181 aaatatggaa cttcaacctg tcacttctct aacagaagaa caaatcaata cacttgcaaa
      241 cgaaatccaa tcttttcatc cagacgtctc acaacaatgg atcaaagaag taattaaccg
      301 acaattacaa ggcgattata caatcccacc tacatactct ccatttagag cagcttggca
      361 aggtattaca gttaatcaaa tgggtgctct attagatact gcaatagctt tagcattagg
      421 aggaactact gcaggccttg caaatctaat taaagtaaaa ggaaaacatg cagcaaaaag
      481 tgctattcgt tcagcaattt ctagatatct aggtagttgg tttgtaaatg atgttgcttt
      541 agaattcgct atgaatttat tatcaccggg gacttattta gcacaattat gggataaaaa
      601 tgatgccatt cctaacaacg gaaggattaa cttttaatca tgagatatac aataaaatta
      661 attgggtata acgtattttc gttgtttata ttctttatct tatcaatgtt gtttaaagtt
      721 ccctttaggt ggtggatcag cattgcaatg acgacttcgt cgtacattgt atataattac
      781 ttaaagaaaa agagagaaaa ggaatgatct tataccagat cattcctttt ctgctttctg
      841 taaatccctc ggagtgtgtt tggcgctttg atacgaagtg tcaaagctgt ataaacgtta
      901 ctatttccaa aaaagtaaca gaaaattcct gctttgtagg actgctttgg gtgagtaata
      961 gacatgtaga ctcaccctat ctcctgatgg cgaaaagaat ttagcaatga tgcaagttgt
     1021 tctgaactcg agaaaaaaca tgccgaaatc gaagaaaagc gtgcagaact tgaagcgaag
     1081 aagcaagaaa tgttgaagct cttggataaa tagaatttag aaaacagaaa aaactaaaaa
     1141 aatgtagggt ttatttccct acattcttaa aataacttag tacataaatg acaatgctgc
     1201 cactgccgtc aatattgcaa aaactatttt ccaaattttt ttattttttc caaaaatctt
     1261 atcaggtaac aagcttatta caagaaaggt aataaacaac accaatttaa gtgtgtatat
     1321 cataaaaaca ccttctttcc tacaattata ttataatact ttatgtaagc gaatacaaga
     1381 gatatgaggt aaccaactac gaaaaaatat aaggctgaag acagtaaaaa ccagcattta
     1441 aagccttcac tgtatctagg atttccctat ttttagacaa gattgaccca aatccacagc
     1501 atttaaaaaa gcgaatttca atcgaaactc gcttttttac aaaatgtatt agtataaatt
     1561 tgtatcttat actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca
     1621 aagcaccgct ttgaggttgc agataaagct gacgtggttt gaagagattt tcgaagagta
     1681 ttagttcaag tgccagatgt cttcattata ctgagcgatt gtacggtcag atgagaagaa
     1741 tcctgcttta gaaatgttaa cgatgacttt atccaaccat gcgtcacggt cttcgtagtc
     1801 agcaagcatt tgctctttga ctttgatgta gtcttccaag tcaagaagag tcatgaacca
     1861 gtctttgttg atcaattcat tgtaaagacg ttccaagcgc tctttgtttc cagctgcaag
     1921 aactgcatca ctaacgatga agtcaaccaa tggtttgata gcttcacgag cgtagaattc
     1981 gcttgatttg taagctgctt ttgcgtaaag gtcgataaca gtttctgaat cttcaccgaa
     2041 gatgtagatg ttttcttctc caaccaactc agcgatttcc acgttagcac cgtccatagt
     2101 accaagtgtc aaagctccgt tcaacatgaa tttcatgtta ccagtacctg aagcttcttt
     2161 agaagcaagt gaaatttgtt ctgagatatc acatgctggg ataaggaaac ttgctgcagt
     2221 aacgttgtag ttttcaacca taactacttg caagtgtgga gctactgctg gatcgttagc
     2281 aataacttct gacatgcaaa ggattaaatg gataatgtct tgagcgattg tgtaggctgg
     2341 agctgcttta ccaccaaaga agattgtgat tggacgagca gggatgttac cagctttgat
     2401 gtcaaggtat ttgtggatca cgtacaaagc gttcatttgt tggcgtttgt actcgtgaag
     2461 acgtttgatt tggatatcaa agatagaatt tggattgatt tccacacctt ggtgttcttt
     2521 caagtgacga gccaatttac gtttgttgtg agccttgatg ctttccaatt tttctttgac
     2581 agctgctttg tcttcataag acaacagttt ttcaagctca tctgcttcat ggtgccaacc
     2641 atctccaaga atctcatcca agtagtgaga caatcttggg ttagcatgca taagccaacg
     2701 acggaaagtg ataccgtttg ttttgttgtt gaacttttct gggtaaaggt cgtagaaggc
     2761 tttcaactca gaatttttca agatttcagt atggagtgct gcaaccccgt taacactgta
     2821 tccgtagtgg atatccatgt gagccatgtg aacacgtccg ctctcatcga tgatttgaac
     2881 agctggatct ttgtactctg ccttcacacg acggtccaat tcttcgatga ttggtaccaa
     2941 gtgaggaacc acttcttgca agaattcaag aggccatttt tcaagcgctt cagcaaggat
     3001 tgtgtggtta gtgtaggcag tcatgctacg aacaattgag attgcttcgt caagatcgat
     3061 accacgtgca gtcaaaagac gaatcaattc aggaatcacc attgatgggt gagtatcgtt
     3121 gatttggaca actgcgtagt cagcaaggtc atgcaagttg cttccttttt cgattgcttc
     3181 gtcgatgatc aattgcgcac cgtttgaaac catgaagtat tgttggaaga tacggagcaa
     3241 ttcaccttga cggtcactat catctgggta aaggaagaga gttaagttgc gagcgatatc
     3301 tgtcttgtca aagttgatac catctttaat aatagaagaa tcaactgaat ccaagtcaaa
     3361 caaacgcaag cggtttttag tcgctgtttc ataaccagta acatcaatat cgtaaagagt
     3421 tgatgtcaaa gtaaagtctg caaatggtac ttggtagcta cggcttgagc gaaccaacca
     3481 gttttgctct gtcaaccatg catttggaat tgtttcttgt tggttgtttt taagaacttg
     3541 ttggaaaaga ccaaagtggt agttaagacc aacaccgtca ccattcaaac caagagtagc
     3601 aattgagtcg ataaagcagg cagccaaacg tcccaaacca ccattaccaa gagatggttc
     3661 caattcaact tcttcaactt cgatcaagtc tttacctgca gctgcaagtt cttttttaac
     3721 atcgtcgtaa agaccaaggt taatcaagtt gtttgacaag agtttaccaa tcaagaactc
     3781 agctgagatg tagtaaactt ttttcttacc agtgttgact ggtttttggc tgcttgcaag
     3841 cttgctgtag ttaagaagag caaggtaaag ctcttcattg ctacattctg caatggtttt
     3901 attgtaacga ttttgtacaa attcttgtag tgataacatg tttagttgtc tcctgattat
     3961 tgtcttattt ctttaaatct accaaatttt cattaattcg gcgataaatt gttgtcaagt
     4021 caagcaaacc ttcctcgaca gctggtgtca attgatcttc agtcatacgc caagaccagt
     4081 ttccaccaag ggtagatggg aagttcatac gagctgcctc atccaattct agtaaatctt
     4141 gcatagttgc aattgccata aagctaactg atgaaaatac tgtacgaagc atagcgtgta
     4201 ccactgtttc gtattcttta cggttcgtgt aacgagccat gtactcacga gtcgcatcat
     4261 caatctcatt acggtaccaa ccaagaaccg tattgttatc gtgtgttcct gtgtacataa
     4321 ctgagttagc aggtgccaag tgtgggctat caatgctttc gtcttctggg ttgaaggcaa
     4381 attgaagaat cttcattcct gggaagccag tacgttcacg caattcgatc acttcatctg
     4441 tcatgaagcc aaggtcttct gcgatgatgt ttagctcacc aagttcttcc ttaacggctg
     4501 caaaaagctt gtagcctgga cctttcaccc actcaccagg tgctgctgta tcggaaccag
     4561 cagggatttc ccagtaagat tcgaagccac ggaagtggtc gatacgaacg atatcgtaga
     4621 ttttgaagct ttcacgcaag cgttcaatcc accatttgta gccgtctttg tccattgctt
     4681 cccagtcata gattgggtta ccccaaagct gaccagttac agaaaactca tctggtgggc
     4741 atcctgcgat acaagtagcc ttaccattga catctgtttt gaagagatgt ggatttgccc
     4801 acatatcact tgaatcttcc gctacgtaga ttggcatgtc cccaacgatt tcgatgtggt
     4861 tgtcgttagc gtaagctttc aatttcaacc attgttggaa gaagaagtat tgagtcacac
     4921 ggtggtaaac caacttgtct gccaattgct cacgatagct ttcaagtgct gaagctttac
     4981 gagcacgagc atctgcatct ggccattcag tccaagcaag attgtcaaaa tactctttga
     5041 tagccatata ctcagcaaag agctcaagcc atgattggtt gtcttgagca aatttctcaa
     5101 aatctttaac atctccgact tcaaagaaac gtttcaccgc tttttctaaa agaggacgac
     5161 gtgcatagta gattttagca tagtcaactt cagacgcatc gctaccaaag tcaactcctt
     5221 caaggtcact tgcttccaac aaaccttgct ccaccaagat atctaaatcg ataaaatgag
     5281 tgtttcctgc gaaggctgag aaagattggt aaggagaatc cccgtaacta gttgctccta
     5341 atggaaggat ttgccagtaa cgttgttttg tacggaccaa gaaatcaacg aagtcgtaag
     5401 cactttgacc aaatgatccg attccgtaag ctcctggaag agaagagatg tgcatcaaca
     5461 caccactttg acgttttttc ataatagcac ctcgtgtgtt aaaataatgg aacgttgcgt
     5521 attttgcaga cgcaaacgtt tgcgttactt ataagtatac tccttttcaa gcataattgc
     5581 aagcgttttt aaaaaacttt tttgattatt tttaaggaga ctaactcatt ttagcaaaga
     5641 taacttaaaa ctatctaatc ctaaggtttg actctcacga gacagtctac aaagtacaaa
     5701 acctccttag ggagcttgaa tttatatgta acaaagcaca aacgtccata gaaaatagat
     5761 agggattaga accagggagg tagcccctcc tggtttccct ctttagacag attccatata
     5821 tttttattaa aaatactgta ttcttatcta tttcatactt ttcttctatt aaaatgcaaa
     5881 attatgctat caaatcaagc ttaaaaattt tttaaaattt ttacaaaaaa tacttgcaac
     5941 cgttttctat ttgtgctata ctaagctcat aaaggaaaac gtttgcgttt ccttatcaat
     6001 aaaacttgct attctttggg aggaatacac tatgtcatct aaatttatga agagcactgc
     6061 ggtgcttgga actgttacac ttgctagctt gcttttggta gcttgcggaa gcaaaactgc
     6121 tgataagcct gctgattctg gttcatctga agtcaaagaa ctcactgtat atgtagacga
     6181 gggatataag agctatattg aagaggttgc taaagcttat gaaaaagaag ctggagtaaa
     6241 agtcactctt aaaactggtg atgctctagg aggtcttgat aaactttctc ttgacaacca
     6301 atctggtaat gtccctgatg ttatgatggc tccatacgac cgtgtaggta gccttggttc
     6361 tgacggacaa ctttcagaag tgaaattgag cgatggtgct aaaacagacg acacaactaa
     6421 atctcttgta acagctgcta atggtaaagt ttacggtgct cctgccgtta tcgagtcact
     6481 tgttatgtac tacaacaaag acttggtgaa agatgctcca aaaacatttg ctgacttgga
     6541 aaaccttgct aaagatagca aatacgcatt cgctggtgaa gatggtaaaa ctactgcctt
     6601 cctagctgac tggacaaact tctactatac atatggactt cttgccggta acggtgctta
     6661 cgtctttggc caaaacggta aagacgctaa agacatcggt cttgcaaacg acggttctat
     6721 cgtaggtatc aactacgcta aatcttggta cgaaaaatgg cctaaaggta tgcaagatac
     6781 agaaggtgct ggaaacttaa tccaaactca attccaagaa ggtaaaacag ctgctatcat
     6841 cgacggacct tggaaagctc aagcctttaa agatgctaaa gtaaactacg gagttgcaac
     6901 tatcccaact cttccaaatg gaaaagaata tgctgcattc ggtggtggta aagcttgggt
     6961 cattcctcaa gccgttaaga accttgaagc ttctcaaaaa tttgtagact tccttgttgc
     7021 aactgaacaa caaaaagtat tatatgataa gactaacgaa atcccagcta atactgaggc
     7081 tcgttcatac gctgaaggta aaaacgatga gttgacaaca gctgttatca aacagttcaa
     7141 gaacactcaa ccactgccaa acatctctca aatgtctgca gtttgggatc cagcgaaaaa
     7201 tatgctcttt gatgctgtaa gtggtcaaaa aaatgctaaa acagctgcta acgatgctgt
     7261 aacattgatc aaagaaacaa tcaaacaaaa atttggtgaa taaaaaattt gttcaagggg
     7321 ggtggaaatc aaatccccct ttgaatttat caatagagac acaaataatt tagctttctt
     7381 ataaaaaagt agtatcctat gaaaggagtt aatatggaaa agcaacaacc tagtaaagca
     7441 gccctgctgt ctatcattcc tgggttagga cagatttaca ataaacaaaa agccaaaggt
     7501 tttatcttcc ttggtgtaac catcgtattt gtcctttact tcctagcact tgcaacccct
     7561 gaattgagca acctcatcac tcttggtgac aaaccaggtc gtgataattc cctctttatg
     7621 ctgattcgtg gtgccttcca tctaatcttt gtaatcgttt atgtactctt ttatttctca
     7681 aatatcaaag atgcacatac gattgcaaaa cgcattaaca atggaattcc agttccacgc
     7741 acactcaaag acatgatcaa agggatttat gaaaatggct tcccttacct cttgatcatt
     7801 ccatcttatg ttgccatgac cttcgcgatt atcttcccag ttatcgtaac cttgatgatc
     7861 gcctttacca actacgactt ccaacacttg ccaccaaaca agttgttgga ctgggttggt
     7921 ttgaccaact ttacaaacat ttggagcttg agtaccttcc gttctgcctt tggttctgtt
     7981 ctttcttgga ctatcatttg ggctttggca gcttctactt tacaaatcgt aattggtatc
     8041 ttcacagcta tcattgccaa ccaaccattt atcaaaggaa aacgtatctt tggtgttatt
     8101 ttccttcttc cttgggctgt cccagccttc atcactatct tgacattctc aaacatgttt
     8161 aacgatagtg tcggtgctat caacactcaa gtattgccaa tcttggctaa attccttcct
     8221 ttccttgatg gagctcttat tccttggaaa acagacccaa cttggactaa gattgccttg
     8281 attatgatgc aaggttggct cggattccca tacatctacg ttctgacctt gggtatcttg
     8341 caatctattc ctaacgacct ttacgaagca gcttatattg acggtgccaa cgcttggcaa
     8401 aaattccgca acatcacttt cccaatgatt ttggctgttg cggcacctac tttgattagc
     8461 caatacacct tcaactttaa caacttctct atcatgtacc tcttcaatgg tggaggacct
     8521 ggtagtgtcg gaggtggagc tggttcaacc gatatcttga tctcatggat ctaccgtttg
     8581 acaacaggta catctcctca atactcaatg gcggcagctg ttaccttgat tatctctatc
     8641 attgtcatct caatctctat gatcgcattc aagaaactac acgcatttga tatggaggac
     8701 gtctaagatg aataactcaa ttaaactcaa acgtagactg actcaaagcc ttacttacct
     8761 ttacctgatt ggtctatcaa ttgtaattat ctatccactg ttgattacca ttatgtcagc
     8821 ctttaaagca ggtaacgtct cagcctttaa actagatact aatatcgacc tcaattttga
     8881 taactttaaa ggcctcttca ctgaaacctt gtacggtact tggtacctca acactttgat
     8941 tatcgcctta attaccatgg ctgttcaaac aagtatcatc gtacttgctg gttatgctta
     9001 cagccgttac aacttcttgg ctcgtaaaca aagtttggtc ttcttcttga tcatccaaat
     9061 ggtgccaact atggccgctt tgacagcctt cttcgttatg gcgcttatgt tgaacgccct
     9121 taaccacaac tggttcctca tcttcctcta cgttggtggt ggtatcccga tgaatgcttg
     9181 gctcatgaaa ggctacttcg atacagtgcc aatgtcttta gacgaatctg caaaactaga
     9241 cggtgcagga cacttccgcc gcttctggca aattgttctt ccacttgttc gcccaatggt
     9301 tgccgtacaa gctctctggg ccttcatggg acctttcggg gactacatcc tctctagttt
     9361 cttgcttcgt gagaaagaat actttactgt tgccgtaggt ctccaaacct tcgttaacaa
     9421 tgcgaaaaac ttgaagattg cctacttctc agcaggtgct atcctcatcg cccttccaat
     9481 ctgtattctc ttcttcttcc tacaaaagaa ctttgtttca ggacttacaa gtggtggcga
     9541 caagggataa tttatccccg ccaccctttt tcattttata ctcttcgaaa atctcttcaa
     9601 accacgtcag ctttatctcc aacctcaaag ttgtgctttg agcaacctgt ggctagtttg
     9661 cactttgatt ttcattgatt attagcaatt gtcactgtaa ataatatcct tgtagcaagc
     9721 aatttttctc ctagacttga aataaagcgc atttctctat ataataatac ttatatagaa
     9781 aacacctttt agaaagatac ctatgcttcc atatccattt tccttttttt caagtatttt
     9841 ggggtttcgt aagcccctgt ccaaacgttt cgggctcaac tggtttcaac ttctctttac
     9901 cagtatcttc cttatcagct tgtctatggt acccattgct atccaaaaca gctcccagga
     9961 gacctatccg ctagaaactt ttatcgataa tgtctatgaa cctctgacaa atgaggttgt
    10021 ccaggatctc tctgaacatg ctacaattgt cgatggcaca ttaacttata ctggaacagt
    10081 tagtcaagcc tcttctgttg tgattggtcc aagtcaaatc aaggaattac ctaagaactt
    10141 gcaactgcat ttcgatacaa atgagctagt catcagcaag gaaagcaagg aactgacccg
    10201 catctcttac cgagccattc agactgagag tttcaaaagc aaagacagct tgacccaagc
    10261 aatttctaaa gactggtacc aacaaaatcg tgtctatatc agcctcttcc tagttctcgg
    10321 tgcgagcttc ctctttggtt tgaatttctt tatcgtctct ctaggagcta gtcttctcct
    10381 ttatatcact aagaaatcac gcctcttttc atttagaacc tttaaagagt gctatcactt
    10441 tatcttgaac tgtttaggat tgccgactct gattacactt attttgggat tatttggcca
    10501 aaatatgaca accctgatta ctgtacaaaa tattcttttt gttctgtatc tggtcactat
    10561 cttttataaa acacatttcc gtgatccaaa ttaccataaa taggagattt ttatgcccgt
    10621 tacgattaaa gacgtggcca aggctgctgg tgtttcgcct tcaaccgtaa cccgtgttat
    10681 tcaaaataaa tcaaccatta gcgacgaaac aaaaaaacgc gttcgcaagg ctatgaagga
    10741 actcaactac cacccaaacc tcaacgctcg tagcttggta agcagctata ctcaggttat
    10801 cggattagtt cttcctgatg actcagacgc cttctaccag aatcctttct ttccatcggt
    10861 tctacgtggc atctctcaag tcgcatctga aaaccactat gccattcaga tagcaacagg
    10921 gaaagatgag aaggagcgtc tcaacgctat ttcacaaatg gtctacggca agcgtgtaga
    10981 tgggctaatc tttctctatg cccaagaaga agaccctctc gtaaaactcg tcgcagaaga
    11041 acagttcccc ttccttatcc taggtaaatc tctatctcct ttcatcccac ttgtcgacaa
    11101 cgacaatgtt caagctggtt ttgatgcgac tgaatatttc atcaaaaaag gctgcaaacg
    11161 cattgccttt atcggaggaa gtaaaaagct cttcgtgacc aaagaccgtt taacaggcta
    11221 tgaacaggcg cttaaacatt acaaacttac cactgacaac aatcgcatct actttgccga
    11281 cgagtttctg gaagaaaagg gctataaatt tagcaagcga ttattcaagc acgatccaca
    11341 aattgatgct atcatcacaa ccgatagcct cctagctgaa ggtgtttgta actatattgc
    11401 caaacaccag ctggatgtcc ctgttctcag ctttgactcg gttaatccca agctcaactt
    11461 ggcagcctat gtcgatatca atagtttaga gcttggtcgt gtttcccttg aaactattct
    11521 ccagattatt aatgataata aaaacaataa acaaatttgt taccgtcaat tgatcgccca
    11581 caaaattatc gaaaaataag agactgggca aagagtcgtt aaaagcaaaa acgcatacta
    11641 tcaggtattg aaaaaacttg atactatgcg ttttattgtg ggaagattta cttccttttc
    11701 tactgaaatt gagtcttttc ccaagatctt tttatactca atgaaaatca aagtgcaaac
    11761 taggaagcta gccgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacga
    11821 agtcagctca aaacatggtt ttgaggttgc agatagaact gacgaagtca gctcaaaaca
    11881 tggttttgag gttgcagata gaactgacga agtcagtaac atatatacgg caaggtgaag
    11941 ctgacgtggt ttgaagagat tttcgaagag tattaatcac taattatcta tctcaacaaa
    12001 tcttcctaga atatgaacat tttccgagac agagacaaag gagcttggat ccacttgtgt
    12061 cataatctgt ttaaattcat taaactctgc acgtgtaatg acagtgatta aaactgcctt
    12121 tctctcgtga ttataggttc cttctgcatc gtggatcatg gttgctccgc ggtgcaattt
    12181 tttatggatt ttttcaatta ccttctctgg atgatttgtc acaatcatgg cctgcatacg
    12241 cttttgctta gtaaagactg cgtctgtcac acggctagag acaaagatgg taatcataga
    12301 ataaagagcg tatttccaac caaaggtcaa acctgctatc agcatgatag ttccatttac
    12361 caagaaagaa atactaccga cattcttacc cgttttcttg cgaatagtca ggctgacgat
    12421 atccgtccca ccactggaga tattgtttcg aagagcaaaa ccaatcccca aacccataac
    12481 aacaccccca aaaagggaat tgataatggg atcctccgtc aaggttgcca cagggacaaa
    12541 ctggataaag aaggaactca tagataccgt gataaaggta aagacggtga acttatggcc
    12601 aatctgatac caagctaaga ccatcaaagg gaagttaatg gcgtagaagc ttagcgaaat
    12661 cggaatatga aaaccaaacc agtgattact caaggcagag ataatctgtg ccagacctgt
    12721 tgcaccactc gaatacacat gccctggttg gaaaaagaaa ttaactgcta ctgctgataa
    12781 aaaaccatag accagagagg ccgaaatctt ctcatcatac ttttctcgag agatactttg
    12841 taagacacgt aaaattttta tctgataagc aaagcggcgc agataatagc gccaccgctt
    12901 aattcgtttt gtttgtttca tcttcttcta cttgtaagct gagttcctct agttgtttga
    12961 gagcgactgt tgatggagct tgtgtcattg ggtcagttgc cttgttgttc ttaggaaagg
    13021 caatgacttc acggatattt tcttctccag caagcaacat gacaaaacgg tcaagcccga
    13081 tagccaaacc accgtgtggt gggaaaccat agtccatggc ttcaagaagg aaaccaaact
    13141 ggtcattggc ttcttcagtt gagaaaccaa gagccttgaa catgcgttct tgaaggtctt
    13201 tttggttgat acgaaggcta ccaccaccaa gctcataacc gttcaagacg atatcgtaag
    13261 caatggcacg aaccttagcc aaatcacctt ctaattcgtg agcagtctct tcctgtggaa
    13321 gtgtgaatgg atggtgggcg ctcatgtagc ggccttcttc ttcagaccat tcaaacatcg
    13381 gccagtcaac cacccaaagg aagttgaact tatcattatc aatcaagcca agctctttag
    13441 caatacgtcc acgaagggca cccagtgttg cattagccac ttcaagcgta tccgccacaa
    13501 agagaaccaa gtccttatct tcaagagcaa gcgctgttgt caattcttct tggataccag
    13561 tcaagaactt ggcaactggt ccgtttaatt ctccatcaac caccttgacc caagcaagac
    13621 ctttggcacc atactgtttg gctacttccg tcatcttgtc gatgtcttta cgtgaatagt
    13681 tgtccgcagc tcctttgacc acaatcgctt ttacagcagg tgcttctgaa aagactttaa
    13741 agtctacacc tttgaccact tctgtcaagt cctgaagcaa catgtcaaaa cgagtatctg
    13801 gcttgtcaga accgtaaaga gccatagcat catcgtattt catacgaggg aacggtagcg
    13861 ttacttcgat gccttttgtt tccttcatca cgcgcgcgat caagccttct gtaatatctt
    13921 ggatttcttg ctcagtaagg aaggacgttt ccaagtcgac ctgagtaaat tcaggctggc
    13981 ggtctccacg caagtcctcg tcacggaaac atttaacgat ttggtagtaa cggtcaaaac
    14041 cagcattcat caagagctgt ttcgtgattt gtggactttg aggaagagcg taaaaatgcc
    14101 ccttattaac acgagacggc actaaataat cacgcgcccc ttcaggcgtt gacttagaaa
    14161 ggaatggtgt ctccacgtcg ataaactcca actcatccaa gtagttgcgg atagagtggg
    14221 tcaccttggc acgaagttta agattttcca acatttctgg acgacgaagg tcaaggtaac
    14281 ggtaacgcaa acgtgtatcg tcatttgcct caatgccatc cttaatctca aatggtgttg
    14341 tcttagctgt gttaagcaca ataagagctg tcacgtttaa ctcaaccgca ccagttggca
    14401 acttatcatt ggcttgctca cgcgcagcga cctgaccagt cacctcaata acaaattcgc
    14461 tacgaaggct ttcagctgtt gccataacct ctgcagatac tttttcaggg ttgataacca
    14521 actgcatgat tccttcacgg tcacgaagat cgataaagat caaaccacca aggtcacgac
    14581 gacggccaac ccatcctttc aaggttattt cttgtccgat gtgttcctca cgaacacgac
    14641 cagcatacat actacgtttc attatttctc tcctctttta ttctgttact attttaccat
    14701 aaaagcgcag gctcttcatg aaaatcatca gaaaagtttg ccagtcttta aaagtcaggt
    14761 gaaagcccta aaaattagcg ctaatactct tcgaaaatct cttcaaacca cgtcagcgtc
    14821 gccttaccgt aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt
    14881 tttgagctga cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgactttg
    14941 tcagtttcat ccacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagtt
    15001 tgctctttga ttttcattga gtataataca aaaatccgat gaacttcacc ggactctttt
    15061 attttgaatt tttgcctgct ttacgctttt cagcgatttc ggctgccttt cgaggcaaga
    15121 caatttccgt tatgtaagcc gtcccaaaac gcagtacacc tgcaatagga gcaaagacaa
    15181 ctgctagata gttatagaag aaatcgcctt tgaaggcata agctagcgct ccaatgatga
    15241 aaaatagaac gactgcctga atcactgcta ataaaattac tcgtttcatg tgacctcctg
    15301 actctattat agcatgagaa tcatcaaaaa gccgactaaa ttattcaaag cgtgaagaga
    15361 aatactgtag accagacctt ttctgctaat gtaagccaaa cccaaactaa aaccaaggct
    15421 aaaatagaca aaaaattgtt gcacatcacc tggaaaatga atcaaggcaa atagaagact
    15481 agataccaga agaaaaatca gggttcgttt actattgtcc tgcttaggaa agagatagcg
    15541 tgctaacatc cctctaaaaa caatctcttc cgtcaaagga gcaaaaataa ccacagcaaa
    15601 gaatgagaaa agtggttgag acaaggtcaa gtctgtcgct atttgctgat ttactgaagg
    15661 atcatctggc aagaagaatt gaacgaccag agataagaac caaaccaaga caggaagcca
    15721 aataaatcga ttaaagccgc tcttctcaat atgaacagga gccttctgat accatttgta
    15781 aatgccgtac acatatactc cagcaaaggc cacatagagt agagtaacag catagggtga
    15841 agcgcctaaa gcaagcgacg cagtcgcgag cccctgaata aagccataga taaataaaaa
    15901 ggatagaagg gctagaagaa tccagccaag gtttttaagt aatttcatag ataactcctt
    15961 tatttgaaat aacgttttac cataggtaac tgcatcacat tgatataaac atggatggct
    16021 cctacaagca agaaagctag taactgaatc tctcctgtca agaaagaaat gataataaga
    16081 aaaatatata aggctggtaa gacatattgg tgtaattgga ataaaattcg aaaactctgt
    16141 tccaaattag cctgacgctc cccttcatca taagaattta tatagttcaa gacatccttt
    16201 ggtgtagcga aaaattccaa atcaaactga cgaacaatcg caatggtttt aaaaagagat
    16261 ttttgagcga ctaagaatac cacaaagagt aagaaagaaa ggaaaaatgt ttgagggttt
    16321 gtatgcaata taatcacctc acttaatgaa ataaaaatag ccaatggaat cgctacacct
    16381 gtaatattaa aagcaatggt tccaaactca agattccgat acatttgcac ataataggtt
    16441 tcattcagat cgtcatccat ttcctcttga tacaaggaat gaaattttct gcttttcttt
    16501 aagaaattga aagtcaaaaa catactaatg aaacctatca gtaaacaaat agctgatatc
    16561 catggcatca aggcttttac atctaaaata atttcgtggg attcgacacg tgccttaaac
    16621 atccctacaa acatgcccaa gacaatagac atcaaaaata acaatctacg tttctttttc
    16681 atattcattc tcctttttca cttgctagat ttttggattt cttttcaatc cattcaatta
    16741 ctgggatgag agcaaagtag acccaaacaa attggtcgct ttgatagaga ttaaaccagc
    16801 ttaggtccat cccaatcagt agaaatacac tgactaataa agctatgacc actacataat
    16861 aaatcacttt atacttgttc atcactcgtc ctcctccaaa cgaaataccg attcgactgt
    16921 ttcgttgaaa atttgagata ttttcagggc aatgataatg gatggggtgt actcatcccg
    16981 ttctagtagg ctaatggtct gtctggaaac ccctgccagt ttggctaggt cggtttgatt
    17041 gagaccatcg cgagctctaa gctcttttag acgattttta agttgcatgt tacacaccta
    17101 ctctccgtca aattcaacgg tttggatatc ctcaatgcgt tgcaacttga atttttcttt
    17161 tcccgtatta tctacacgtc gtagctttac ccattcctca tcaacatcca caacttccca
    17221 gttatctggc ccaatataca ctcccgttat aattggttcc tttccaatca tttcttgaaa
    17281 taatctcgac atttctgcgt ttcctttctc ttttcgctca agttttttga ttttattctc
    17341 tagtttcttg atttttttag aattattaga ataaaagaaa atcataaata gtataaatcc
    17401 tagtacccac atcatagctc ctttcttctt ttctcttaac ttgcttacat cgtaacacat
    17461 ctttttcttt ttgacaagta tagttgtcaa aaagtttatg atttttgtca ttttgcaaag
    17521 gaaaaaggtc aggagtaagt tcctgaccac tttatctatc attaatactc ttctaaaatc
    17581 tcttcaaacc acgtcagctt catctacaac ctcaaaacca tgttttgagc aacctgcggc
    17641 tagcttccta gtttgctctt tgattttcat tgagtataaa atcctagttt ttcaaagatt
    17701 tctgagaagt tttggctgat tgtctcaagt gacacttgca cttcttctcg ggtttggttg
    17761 ttcttgaccg tcacttgtcc gctttcgact tcgctctctc ctagggtgat gagggtctta
    17821 gccgcaaaga catctgctga cttgaactga gctttgagct tacggttgag gtaatcacgc
    17881 tctgctttga aaccttgttg gcgaagagct tggactagtt ctagggcttt gacatttgct
    17941 ccatcaccca agactgcgat atagacatct agggcgtttt cgatagggag ggccacgcct
    18001 tgtttttcaa ggatgagaag caggcgctct acaccaagcc caaaaccaaa tccagcagtt
    18061 tcagggcctc caaagtaagc aaccaaacca tcgtagcgac cacccgcaca gactgtcaag
    18121 tcattgccct caatctctgt gataaactcg aaaatggtgt ggttgtagta gtccagacca
    18181 cgcaccatat tggtatcgat gatgtaatct actccaagat tttccaacat ctgacgcaca
    18241 gcatcaaaat gagtttggct ttcttcatca agaaagtcca agatagacgg cgcattctct
    18301 actgccacct tgtcttcttt ttccttagag tccaagacac gaagaggatt ttcctccaag
    18361 cgacgttggc tatccttaga caaggtctcc ttgagcggtg tcaaatagtc aatcaaggct
    18421 tggcggtagg ctgcacggct ctcaggattt ccaagagtgt tgaggtgcaa tttgacacct
    18481 tgaataccga tttccttcaa aaaataggct gccatagaga ttgtttccac atcggtagct
    18541 ggattgctag agccaaaaca ctcaacacca atctggtgga attggcgcaa gcgccctgcc
    18601 tgtggacgct cataacggaa cataggtccc atatagtaga acttgcttgg cttttgcact
    18661 tctggggcga agagtttatt ttccacatag gaacggacaa cgggcgcagt tccttctgga
    18721 cggagggtaa tatgacggtc acccttgtca taaaaatcgt acatttcctt ggttacgata
    18781 tccgttgtat ctccgacaga gcgactgata acctcgtaat gctcaaaaat aggcgtgcgc
    18841 acttctgcat agttatagcg cttgaaaatc tcacgggcaa agccctcaac gtactgccac
    18901 ttagcagatt cagcaggtaa aatatcctgc gttccttttg gtttttgtaa tttcataggg
    18961 aatcctcttt aaacttaata gtcttatttt accacaaata aagggattaa aacagtaaga
    19021 aaaaaattag gatttagata tcatttttga gattaagaat tgtcaaaaaa atagctagca
    19081 aggaaagacc aacaaatagc atccaagtca actgtatatt ccatacggct actagtgaaa
    19141 aacaagctgt tcccacaggt atggataagg taaacaatag acctaaaaaa ttactagtac
    19201 gagctagaac ctctggagct agatttttca tgagcatggc actaatcttt ggttgaactt
    19261 taccagacac atacagggta aagaagagaa atagcaaacc aagcacgact tgattgaata
    19321 aattagccaa accaactaga ctaagtccta cggtcgccca catcatcaat ctaggcaagg
    19381 actgcttccc aaaataatca ttgcccgtaa ggctactgat gatgattgct actaaaacac
    19441 agaattgatt gataaatagt gcctctgtat aagaaaaatt caagagagaa tggctcaaaa
    19501 agaagatatt ataaattcca cccaaagcgc cacccaagga attaataagc aagacagcaa
    19561 agagcataaa accaaagttt ttctgcccac ttttaagaaa aacgagacgt aaatttcggt
    19621 aaattgttag gaactggtct ttgatagaaa gcttctcatt ttttaagttt tcaccatcag
    19681 cagatgacat tgacaggctc aatttgcttt ttcctaaaaa gaggatagtg gctgatacta
    19741 ggaaaaagca ggcattgatt cccgcaacga gagaaaaatt gttgaccgat agagctaaga
    19801 gccagactcc gaaagcttga ccaccaatag ctgaaatata ggtgatgaac tgtgaaaaag
    19861 aataagcctc catcagatca tcttcagcta ctttttcctt aataagaggc atgcgcaagc
    19921 cacctgcaaa atcactgatg acatcactaa tgacattgat caaacacagg ctagaaaagg
    19981 caaagagact agcttgctga acaactaggg ctgctaggaa aaatagaacc gcctgaaaca
    20041 aaccgctata gaccatccat ttgaccttgt ccctcgtgta atctgcccga atccctacaa
    20101 aaactgtaaa gagggtcgga agaatcatga caatattcgc catagcaaca gcaaaagatg
    20161 cttgtgacaa ggtcgatgca tagacgataa agaccaggtt gaaaatcgaa gcaccaaaag
    20221 cattgaagaa gcgtgataaa gtcaaaattc tataagctgg atttctaaac aatagtttca
    20281 tagataatct cccttcttgt tctaagttct acatcaagaa tagctttagt atccccaatt
    20341 attcacccca aatctaaaaa ccatccagaa tccttgcctt agcttagatc ctggatggtt
    20401 tcttttttca cccaatgggt gttttttact agacaaaaaa gagtttcccc tttatggtat
    20461 aagtgtagaa aaaaacacaa aaagaaagga aactcacatg aacagtttac caaatcatca
    20521 cttccaaaac aagtcttttt accaactatc tttcgatgga ggtcatttaa cccagtatgg
    20581 tggtcttatc ttttttcagg aacttttttc ccagctgaaa ctaaaagagc ggatttctaa
    20641 gtatttagta acgaatgacc aacgccgcta ctgtcgttat tcggattcag atatccttgt
    20701 ccagttcctc tttcaactgt taacaggtta tggaacggac tatgcttgta aagaattgtc
    20761 agctgatgcc tactttccaa aattattgga aggagggcag cttgcttcac agccaacctt
    20821 atcccgtttt ctttccagaa ctgacgagga aacagtccat agtttgcgat gcctcaacct
    20881 tgaattggtc gaattctttt tacagtttca ccagctaaac caactcattg tagatatcga
    20941 ttctacccat ttcacaactt atggcaagca agaaggtgtt gcttataacg cccactatcg
    21001 tgctcatggc tatcatcctc tttatgcttt cgaggggaag acaggttatt gtttcaatgc
    21061 ccagcttcgt cctggtaatc gttattgttc tgaagaggca gacagcttta tcacacctgt
    21121 tttagaacgg tttaatcaac ttctctttcg aatggatagt ggctttgcga ccccaaaatt
    21181 atacgattta attgaaaaaa cagggcaata ctacctcata aaactcaaga aaaatactgt
    21241 tctgagccgt cttggagacc tttccctccc ttgcccacag gatgaggact taaccatctt
    21301 gccccactcc gcctactcag aaactctcta tcaagcagga tcttggtcgc acaagcgtcg
    21361 tgtctgccag ttctctgaac gaaaagaagg aaacttgttc tacgatgtta tttctctcgt
    21421 tacaaatatg acgagtggaa caagccaaga ccagtttcag ctttatcgtg gacgtggtca
    21481 agccgagaat ttcatcaagg agatgaagga gggatttttt ggcgataaaa cggatagttc
    21541 aaccttaatc aaaaacgaag ttcgtatgat gatgagctgt atcgcctaca atctctatct
    21601 ttttctcaaa catctagctg gaggtgactt ccaaacttta acaatcaaac gcttccgcca
    21661 tctttttctt cacgtggtag gaaaatgtgt tcgaacagga cgcaagcagc tcctcaaatt
    21721 gtctagtctc tatgcctatt ccgaattgtt ttcagcactt tattctagga ttagaaaagt
    21781 caacctgaat cttcctgttc cttatgaacc acctagaaga aaagcgtcgt taatgatgca
    21841 ttaaagaaca gtcgagatga aaaaatcgtg tgacgaacca agggaggagt ctgccctttt
    21901 gaggaaatct agcgaggaaa aacgatactg gaacagcaga aagtaaaact gacctcatga
    21961 ggaggaagaa agtggctcat gaggtcaggg gttttgtaag ttacatctag ttgagagagg
    22021 tatgaatgat ttgggagtat agcacttatg aatttgcgaa acgattcttt tgaatatatg
    22081 caacaaacaa aatgagcaaa aaaacgatag aacttttgta atgacctgag agagggcaac
    22141 agttctatcg cttcaaattt tttcttggtt tgcagatact caacattcgg tcgtatttct
    22201 atagtattcg gcagatttat tacagccaag catctcaaaa atacggacag catcctccat
    22261 ctttttctga ccttccttga ctctaccttg cttgctatca aggagacctt ctgcccacag
    22321 ataaacaatt cggaaatagg tctcattttc cttgtagaaa tgctcttcga taacacgttt
    22381 aaaataatag gcattggtaa attcttcaca ctcaatacta gctaaaaagc cattcaatag
    22441 tatagtataa aaaaggtttc gattgccaga catttccatt agaaaatcgg atttacgtac
    22501 catttctcgt acatatctag taaaaagaga aacagataaa aatggagaac tgactgaaaa
    22561 taaattgagt tcatagattc cccagatctc ggtagaaaac aaataatcat gaaggacttt
    22621 tccttcctct gctgttaact ctaccctttc atccatgctc ttcatataag acttgatcat
    22681 aatggcattt agaatatgtt tctgtttgtt gtgagaatgg gcatgctttt gatactccct
    22741 gcgatataag tcctcaagag gtgctacatt ctttggttcc aagacatctg taatttcttt
    22801 tctcaactca gaatctgtat catactggaa acctctcgcc agaaagagga tctcctccac
    22861 actggcagat atattttcca gagcaaatag aaacttttcc accgaaagct cactctgacc
    22921 tgtttcaaaa cgggacaaca tagacggcga aaattgtcct ccggttgctt gtctcagtga
    22981 gatatttctt gactctcgta attgtctaaa gacttttcca atctgctcca tagacttccc
    23041 cttgattccg tattttcttc attttatcat atttttcaga aaattcatca aaaacttgcc
    23101 aaattgtcag aattatgaga aaatagagga tatttatcac gtggagggac tgctatgaga
    23161 gacgatatca aaatcaatga ccgtgctttg gccttgcaag accaaatcat cgaaaaacta
    23221 gagaaagttt ttgatacaga tgtggaattg gatgtttaca atctaggtct gatttatgaa
    23281 atcaatctgg atgaaacggg gctctgcaag attgtcatga ccttcaccga tactgcctgt
    23341 gattgcgccg aaagcctgcc tattgaaatc gtggcaggtc tgaaacaaat cgagggtatc
    23401 aaagatatca aggttgaagt tacctggtcg cctgcttgga aaatcacacg aatcagtcgc
    23461 tatggccgta ttgcccttgg actaccacct cgttaagcag gccaatcatt tttaaagatg
    23521 aaaaaagcaa gccaaaaatg gcttgctttt tgatactcaa tgaaaatcaa agggcaaact
    23581 agaaaactag ccgcaggttg ctcaaaacac tgttttgaag ttatggatag aactgacgaa
    23641 gtcagcccaa aacactgttt tgaggttgtg gatagaactg acgaagtcag taaccatacc
    23701 tacggcaagg cgacgttgac gtgatttgaa gagattttca agtatgagtt tattttttac
    23761 ctgacttgtc catattccag aagtctgtca cggctccgcg tgaagcagat gatacgatat
    23821 gggcatattt accgaggaca ccacggctgt aaagtggtgg caaggttgtt tctgccttgc
    23881 gtttttcaag ttcttcttcg gatacggcca tggaaatttc tttggtatct tggtcaaccg
    23941 taacgatatc gccggtacgg agataggcaa ttggtccacc atcctgagct tcaggagcga
    24001 tatgtccaac aaccagacca taagtaccac cagagaaacg tccgtccgtc aagagggcca
    24061 ccttatctcc ctgaccttta ccaacaatca ttgaagaaag tgacagcatc tcaggcatac
    24121 caggaccacc tttaggtcca acaaaacgaa caacgactac atcgccatca acgatttcat
    24181 ctgtcagaac ggcctgaatc gcatcttctt ctgagtcaaa gaccttagct ggcccaacgt
    24241 gacgacgcac tttaacacct gataccttgg caactgcacc gtcaggagca aggttcccgt
    24301 tcaagatgat aagcggacca tccgcacgtt ttggattttc aagtggcatg ataacttttt
    24361 ggcctggagt caagtctgca aagtcagcca agttttcagc tacagtctta ccagtacatg
    24421 tgatgcgatc tccgtgaagg aaaccatttg ccaacaagta cttcataacc gcagggacac
    24481 caccgacttc gtagaggtct tggaagacat actgaccaga tggtttcaag tcggccaagt
    24541 gaggcacacg ttcttgaatc gtattgaagt cctcaagtga caagtcaaca tttgcggcat
    24601 gggcaatggc gagcaagtga agagtggcgt ttgtagaacc accgagagcc atcgttacag
    24661 taatagcatc ttcaaaggct tcacgagtca agatatctga tggtttgaga ccaagttcca
    24721 acatcttaac aacagcacgt cctgctgctt cgatatcttc tttcttatca gctgattcag
    24781 ctgggtgaga ggatgaccct ggcaaactca tccctagaac ttcgatagca gttgccatgg
    24841 tattagcagt atacatacca ccacaaccac cagggccagg gcaggcatta cattcaagac
    24901 gtttcacgtc ctcagctgtc atgtcaccgt ggttccattt tccgatacct tcaaagacag
    24961 aaaccaagtc gatatcttta ccatcaagat ttcccggtgc aatagttcca ccataggcga
    25021 aaatagctgg gatatccata ttagcaatag caatcataga tccaggcatg ttcttgtcac
    25081 agccaccgat agcgacgaag gcatccacgt tgtgaccact catagccgcc tcgatggagt
    25141 ccgcgatgat gtcacgagat gttagagaga aacgcatacc aggcgttccc atagcgatcc
    25201 cgtccgctac ggtaatggtt ccaaactgta caggccaagc gcctgcagat ttgacacctt
    25261 ctttagccag tttcccgaaa tcatgcaagt gaatgttaca tggtgtattt tccgcccaag
    25321 tcgaaatcac tcccacaatc gatgtttcaa agtccttatc tgtcatacca gtcgcacgaa
    25381 gcatagcacg gttaggtgat ttaaccatgc tgtcatagat gctactgcgg tgacgtttat
    25441 ctaattcagt catcttatcc ctcccatttc agtttttact attatagcac aatttaaagg
    25501 caatgaacag aataaaattc ttgaattttc agaaaattct atacgcatgt gaaatattta
    25561 aaattaaaaa caacaaagcg gattagtgca ctttctgatg accagaatat gctttttaat
    25621 ccgctttctt taaataacgt actgtaattt ttacagaaat tctttcagat aagtgtattt
    25681 aacatctatc ttgcattata aatttctaga accttctctt ttatattcga ttcactcaaa
    25741 ccatactcat taagaagata atccattttc cctacttgac cgaatctttc ttgaacaccc
    25801 atccgatgaa tttttgttat tccatcatca gagaataatt cacataaagc actgccaatt
    25861 ccacctatct gattgtggtt ttctacagta aatatagttt ttccacttaa cattgttttt
    25921 atctgttctg gtatcggttt gattctaaat aaatctatca cacttactga ataacctaat
    25981 ttagacagtt catctgcaac tcgaatactt ggagcaacca ttataccaga agcaacgatt
    26041 acaagatctt caccatgcct taactcaatg tagcctttag aaaaatcttc tccaccttga
    26101 tacacaggaa ctggagcttt tctaattgtt cgaatatatt ttagtccttt taagtctaat
    26161 gtctggttca atatttcacg aaattggata tcatcagttg cttcaaaaat gatcgattta
    26221 ggaattaaac gtaacaatcc aatttcttca aatggcatat gtgtcccacc attcatttct
    26281 gcagttactc ctgcatctga tccaatcaca gtggcatcca attgtgcgta tccaagagaa
    26341 ataaataatt gatcaaatac tcttcgtgaa gcaaaaggac caaatgtatg aagataaggt
    26401 ctaaacccct gaatagacaa gcctgctgca aggccgacca tttctgcttc cataatccca
    26461 acattcacat aacggtctcc aaagtccttt tcaagattat gagtagccat cgaacttgac
    26521 aaatcggctt ctaagactac tatatcagaa tcactttgat tagcctctaa aaggaagtct
    26581 ctatatacat gccgtaattc tttcgtgctt ctcatcattc tgtttcctcc aattcctgac
    26641 ttaatctttc tacaactgag gttaacattt gtctctcctc tacagtaggg cgaagatgat
    26701 gattggattt catttcttcc agctcttgaa ccccttgacc tttaatagta tctaatacaa
    26761 tgcacttagg tgatgaatta tttgactgtt ttaattggac aatcccttca taaatttctc
    26821 taatatctga acccttgacc ctaatggatt caaatccaaa tgctgaaaat ttttctacga
    26881 aatcacctgg attacaaata tcctttgtaa aaccatctaa ttgttttttg ttatcatcaa
    26941 caaatacaat taagttggat aactgttgat gagaagcaaa ctgtatagcc tcccaacatt
    27001 gtccctcatt taactcacca tctccaacaa tagcataagt ataaaaggga ctctttctta
    27061 ttctctgacc atatgcaagt ccagttgcaa cactaattcc ttgtcctaaa gagcccgttg
    27121 tcatatctat gcctggcgtt agatttctat caggatgaga cggtaatttg gttccatttg
    27181 tatttaaaga atataagaat tctttgtcaa agaaaccatt caaatagagt gtactgtata
    27241 gagctggtcc tccatgacct tttgataata tgaaataatc tctatctcgt gctgcaaata
    27301 tttctggagt cattggcatt atttcaccat aaagcactgc taaaacttct acgatagaca
    27361 gacttcctcc gtaatgtccg aatccaagat gattcaatgt tctaagagta tttaatcgga
    27421 tgttagtcgc aaattttctt aacccatctt ctctattttt acttaaaatc atcccttatt
    27481 cctcctttgc agatggcttt ttaataaagg atactccaaa cataactgct agaataagaa
    27541 caagaccaat cacaatgcct gcttgtgagc caaattgatt taacattcct aaaataattc
    27601 ctgatagacc aaaatctgca tctgagaaag ttgatccttg gaaaccaagt cctcccaaaa
    27661 ctggcattaa aaagactgga agaaaactga ttaaaatacc ttgtaaaaat gctccaatag
    27721 tggctccacg aacaccacca gatgcattcc caatgacacc tgcagtcgct ccacagaaga
    27781 aatgaggcac aacacctggt aagataacaa ccgttcctga agcaatcata attgccatac
    27841 ttactaaacc accaataaaa ctagagataa atccaattag aactgcattg ggtgcataag
    27901 tataaacaat cggacaatcc aaagcaggtt ttgaattagg tacaagacgc tctgaaatac
    27961 ctttaaaggc tggaacaatt tcgcccaaaa taaggcgaac acctgctaaa ataacaaata
    28021 cccctgctgc aaattgacct gctaattgta aagcataaac tagaccactt gtaccactac
    28081 tgatttcttt ttctatatat tctgaccctg caaagatagc tacaataatg taaataactg
    28141 ccatggataa agtaatacta acagtactat cacgtaaaaa agctaaactc tttggaaatt
    28201 taatgtcctc tgttgatttt gatttgtcac cgataaggct accagtaaaa ccactcaacc
    28261 aatatcccaa agaactgaaa tgacctaaag ccaccttgtc atttccagtt aattgaacca
    28321 tatatttttg aacaaatgct ggggaaatac tcataataat accgagtgct aatcctccta
    28381 gtaagataag aggcaagcta gtaaagccag caactgataa aatgaccgca atcatacatg
    28441 ccatatatag agtgtggtgc cctgttaaaa aaatatattt aaatcgagta aaacgagcga
    28501 ttaagatatt gaacaccatg cctgcaaaca taatcagtgc agtagctgag ccatatgttg
    28561 ttaaagctac agctacaatt gcttcattat tcggcacaac gccagataaa tgaaaagcat
    28621 gctcaaacat ggtaccaaat ggatttaaag aattttgtac aattcctgca ccaccagata
    28681 caactaagaa accaacaaag gtcttaattc cacctttaat aatatcaggt aatttcttct
    28741 tctgaagaac taatcctaaa attgcaatta aagctactaa aatagctggt gtactaacaa
    28801 tatccaatat gaacttcatc atgacgctag cctcctatat aagtcctttt tcttcacaaa
    28861 gtttagtaat taattctcgt agttcatcca tatcaataat actatttaag atacgaacat
    28921 ctccaagatg actagctgaa tcagctagat cacgaccaac aatccaaata tcagctgcat
    28981 ttggatctgc tccacctaaa tcataatgtt caacttctac atctgaaaca ttcaaatcac
    29041 tcaatacaga ttcaatattc atctgtacca taaaacttga acctaatcct gaaccacaag
    29101 ctgtaccaat ttttaacatt atctaatcct cctgtttaat tatcatttta atgtcatcat
    29161 agttttttga tgatattaaa gtttgaacat gatttttatc tcttaaaatt gttgttaaat
    29221 gtgacaaagc ctttaaatga ctctcattat caatggctgc aatacaaatc aacaatctta
    29281 cctcttgttc tggattatcc aataaataaa tcggttcttc caaaactaac attgacattc
    29341 ctatttcatt cacaccttca tctggccgag cgtgaggaat tgctactccc ttccctaaat
    29401 taataaaagg cccaaactct tctacttttt gaatcattgc ctcagggtag ttctcagtta
    29461 tcttatcttg atccaaaagc ggtttagctg ctaaacgaat cgcctccttc catcctaatt
    29521 tttgcgaact aacctgatag gtttctttgg taatgagttg ttctagcact ggtacaattt
    29581 cctttctatc atttttttgg taaagataat tctttaacgc caatcttaat tccaattctt
    29641 gtgtaataat tccatatctt ttgacaatat tcagaatttg ttcaatctca aaatccccat
    29701 actctaaatt cggaaaatct tttaacacta gttctactag ttgtattgct tgctcttcag
    29761 tcatcataac cgaaactaga taatttggct tttctgtctc cacctttatg gtagaaaaaa
    29821 ccatatcata gtcactacta gctttcacct gtaaatcatc aatctttgag gttcctataa
    29881 actcaatttg aggaaataat gctaatagat tctcttttaa catcaatgaa gaactaacac
    29941 cattaggaca aatgattgct gctttatacc atttttgagg caaagtatct gctttcttta
    30001 aataacctcc gaaatggata acaaaatatg ctgtttcact atcaggtatg ggattgtcaa
    30061 tagcgtccat caagggcatc aaagaatctt tgactaattc aaataaatca ggataatatt
    30121 ctttaacatg caatacatat tcatttgaac taggtaggcc gaactttaat ctatagtaag
    30181 ccggtataag gtggcggcga agattttctc tcaatccttc cctttgttta aaatgtaaca
    30241 aagaaatatc ttccattcta cttataatag cctctgttaa ttgattaaag taaaccggag
    30301 caacatctac ttcaccttca aagcaacttg ataataaaac tgtgatatag cgataatcat
    30361 cctcagaaaa caccgtatct ataattccca aatcaaccac tgtatccaat aaaatagtgg
    30421 ttatatcttg aataacagga gatactaatg tctctgaaag acatactctt tcaacatccc
    30481 tttgatacct acacagaatg aatactaaac cgaaaaggca aacttttaat tgattaacaa
    30541 taggtactag ttgtagcttc tcataataat ctttaactac ctgatttatc aaatcataag
    30601 ttaatgaata cccccaactg gataaaacat aatccaaacc ccaaatccct atggaggatt
    30661 ccagcaactc actaaccatt tgaaaagcta agcggtgctt attccactct gaaccgtgta
    30721 aagtataacc ttttgctcta ctgtacccta gctccaaatc attatctaac ataatctttc
    30781 ttaatgattg aatatcagat aaggttgtat tcttacttac tttcaaaaag tcttggtaat
    30841 gattattcga tataaaatct aatcggcaaa aagtgtaaag atagattaaa gctaagcgag
    30901 tcgactttgg taaaaccaat tcatccgact taataatatc tgtcaaagac tgcttcgtac
    30961 gatttgataa actatagcga ccttgttttt tatccagcac tatcccttta ttagctagat
    31021 aaggcactaa ataatctatt ccttctttga cttcctttat aggtaagctc accttaacag
    31081 ataattcata taatgatagc tcacaatgat ccatcaaagt catcaaaata actagtgctc
    31141 tataatcaaa catttctctt cctttctaac tacaattata gtgtaaaaat tataagaata
    31201 aaagagtatt tgagcacaat ataatctcac cttttaaaag aaattattgg gctgaaagtt
    31261 tttagtgatt attttcaact ttctattgac ttttaaaaac aaagtgatat catttagata
    31321 tcaaataatg ggaggtcatt cttatgactg aaaaactaat caattcaaaa ccaaatggtg
    31381 tattcgcatt gattctcatt gagttgacaa tcgtacttgg tgtctttata tttataatgg
    31441 gtgttggttc ggaaaacatt tttggaatta ttatcggacc tttactaatc gtaattgcag
    31501 gtctagctca tgctggttta aaagttgtca aacctcagga agctctggtt ctgacactct
    31561 ttggtaacta tacaggtacc atcaaagaac ctggttttta ctttgtcaat cccttcagcg
    31621 tagcagtcaa ccctgcaaac cacactcgac ttggacaaag tggtgatgtt agcacaaaat
    31681 ctcctttttt aggagctaaa tcatcaaatg acaatgatgt aaatcttgaa attggcaaga
    31741 aacagatttc cctcaaagtc atgaccttga gcaattctcg tcaaaaaatc aatgattgct
    31801 taggaaaccc tgtagaaatc ggtatcgcgg taacttggag agttgtcgat accgctaagg
    31861 cagtcttcaa cgttgataac tacaaagaat atctttcatt gcaatgtgat agcgccctcc
    31921 gtaatattgt ccgcatctat ccttacgatg tgtctcctaa tgtggatact acgggtgacg
    31981 ggcaagcaga tgaaggtagt ctccgtggct ctagcgaaat tgttgctaac cgtattcgtg
    32041 aagaaatcca aagtcgtgtt gaggatgctg gcttggaaat ccttgaagca cgtatcactt
    32101 acctagctta tgctccagaa attgctgccg ttatgcttca acgccaacaa gcatctgcca
    32161 ttattgatgc acggaagatg attgtagatg gtgctgtagg aatggttgaa atggcactag
    32221 aacggctcaa tgaaggggaa ttggtagaac ttgacgaaga acgaaaagct gccatggttt
    32281 caaatctcct tgtcgttctt tgtggcaatc atgatgcaca accaattgtc aacacaggaa
    32341 gtctttacta aagatggctg aagctaaaaa aaagcagatc ccccttcgac tctcaacaaa
    32401 gttatacgct gcactcgcat catgggcaga agacgacttt cgttcagtca atgggcaaat
    32461 cgaatatctc ctcacagagt gtgtcaagca acggaagaaa gacggaaaat acgtatcaga
    32521 aaccattgac gaaccatttg agattgatat ttaacatctt ctcctgtctg acaagtcgga
    32581 caggagaatt ttttcatctt tttttgtcaa gtaactttac tttacaaaaa aaatgtgtta
    32641 tcctagtatg gttgatgaaa atcagtagat tgaatcgaat ataaatacct aaaggagaaa
    32701 tcaaaatggc agtacctgca cgtcgcactt caaaagcgaa gaaaaacaaa cgtcgtacac
    32761 actacaaagt aacagctcca tctgtaaact ttgacgaaac tactggagat tactcacgtt
    32821 ctcaccgtgt atcacttaaa ggatactaca aaggacgtaa aatcgctaaa gctgcatcag
    32881 ctgaataata gaagggagat accatgcgcg taaatattac acttgaacac aaagaatctg
    32941 gtgaacgctt gtaccttact tctaaaaaca aacgtaacac tccagaccgc cttcaattga
    33001 agaaatactc accaaaactt cgcaaacacg ttgtgtttac agaagttaag taagcattca
    33061 atacgaatca atacagaaga aaaacgcttg atcagcgttt tttctttttg ttaatttcac
    33121 tatattgcaa gggaaatcat tcgaatgact acatttttca ctaccttttt tgaaataaga
    33181 atacggattc ggacgattaa aagtaagaga aactcaaatg taagttgggg ttcctttttt
    33241 gtatattttt caaataacgc ctctacacta tttgtagcaa attcaccaac tacagttgta
    33301 tcttagttaa aataagttag aatatgtaag tgagtaccag atataccaag acatcgtcac
    33361 catctaaggt atattcaaaa tacaaaagtt gaccaactag atttctgaat atccttatat
    33421 atccattctt aaaattggtt taaatagcgt agtcttttaa actagttttg agaatccaaa
    33481 aaatcttcct acatatgtaa gaagattttt tagttcagaa tgattagatt tagctaatgg
    33541 atacctatcc tacccactca ccgttagcat tgactcgata gccatcgggt gtagtcgtgt
    33601 ttactaataa atctcctgaa ttataggtat agtaccattt accagaaact gtaa
//
LOCUS       DAVLFB010000010        33172 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLFB010000010 DAVLFB010000000
VERSION     DAVLFB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.010"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..2480)
                     /locus_tag="VXD58_000412"
     CDS             complement(<1..2480)
                     /locus_tag="VXD58_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV0675162.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGEYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMTTGRSLELDNVFITQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVARLEAFKSQ
                     TTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELDGASTLLAQE
                     AKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEA
                     LSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEELASRIASVQA
                     SGRNLFLNSLFKQDISKTGIWTTSTYTATIDSESKYLGYNALKIIGLNPSGRDGGNPK
                     VTYPVLGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIK
                     RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQI
                     STVESTFKQRVNSLEAGVNRLTEGLRTKV"
     gene            complement(2485..2835)
                     /locus_tag="VXD58_000413"
     CDS             complement(2485..2835)
                     /locus_tag="VXD58_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675163.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     LKPWSGAKFNLVER"
     gene            complement(2844..6497)
                     /locus_tag="VXD58_000414"
     CDS             complement(2844..6497)
                     /locus_tag="VXD58_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675164.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSSSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGDTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIERIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNIGNISEAVGNIAQDFSSTFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVAPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPILALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSLIVGGIKNLGLAIKALTFGKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICRAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDIFQKAWTGLTNIFSKLGLWFGERWADVTSVLANVSSWFGN
                     MFTSAYNAVKNAFSSIGGFFSGVWSTVQSIFVNAGQKVGSAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGISPQGGFSSDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            complement(6484..6834)
                     /locus_tag="VXD58_000415"
     CDS             complement(6484..6834)
                     /locus_tag="VXD58_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675165.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            complement(6873..7253)
                     /locus_tag="VXD58_000416"
     CDS             complement(6873..7253)
                     /locus_tag="VXD58_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675166.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSLEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVDK"
     gene            complement(7258..7671)
                     /locus_tag="VXD58_000417"
     CDS             complement(7258..7671)
                     /locus_tag="VXD58_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0675167.1"
                     /translation="MLANGITLSYGETKDSYTKLVGLKEVPEFGIELEKVENTTLEDT
                     VKKYEFGIGDIGELEYKFSYNNSSATAPYRVLRKAADDKKKLYFEQAYPDGTKVIFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSNLTFTDGIGG"
     gene            complement(7674..8042)
                     /locus_tag="VXD58_000418"
     CDS             complement(7674..8042)
                     /locus_tag="VXD58_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675168.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGIVDLHSELVFQFRMEN"
     gene            complement(8039..8554)
                     /locus_tag="VXD58_000419"
     CDS             complement(8039..8554)
                     /locus_tag="VXD58_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV0675169.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            complement(8529..8867)
                     /locus_tag="VXD58_000420"
     CDS             complement(8529..8867)
                     /locus_tag="VXD58_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675170.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYAKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            complement(8848..9159)
                     /locus_tag="VXD58_000421"
     CDS             complement(8848..9159)
                     /locus_tag="VXD58_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV0675171.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGEHSRTEGGIAVVYGENGLSTDLLQRIRMHRLARVAGHVFEA
                     E"
     gene            complement(9161..9349)
                     /locus_tag="VXD58_000422"
     CDS             complement(9161..9349)
                     /locus_tag="VXD58_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675172.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGEELEITEERYAELADNIASNGVA
                     ISDVLEEIFPKPEFLEED"
     gene            complement(9359..10384)
                     /locus_tag="VXD58_000423"
     CDS             complement(9359..10384)
                     /locus_tag="VXD58_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV0675173.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKPSTQGKVVNRAKAWKTGTTYKEGDTVTHADKVYVAVKDISNSSTAPDTDTTNW
                     KEKTGKK"
     gene            complement(10407..10931)
                     /locus_tag="VXD58_000424"
     CDS             complement(10407..10931)
                     /locus_tag="VXD58_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV0675174.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDSVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            complement(11192..11371)
                     /locus_tag="VXD58_000425"
     CDS             complement(11192..11371)
                     /locus_tag="VXD58_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675175.1"
                     /translation="MDKAKIGITNVEFSGTGGIESATVKLELNIYGADTFSAIELLPK
                     ILTDIHSLSYEVDWL"
     gene            complement(11413..11826)
                     /locus_tag="VXD58_000426"
     CDS             complement(11413..11826)
                     /locus_tag="VXD58_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0675176.1"
                     /translation="MIDIALAIAKKAHAGQVDKADVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            complement(11823..12032)
                     /locus_tag="VXD58_000427"
     CDS             complement(11823..12032)
                     /locus_tag="VXD58_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675177.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSIDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            complement(12553..13671)
                     /locus_tag="VXD58_000428"
                     /pseudo
     CDS             complement(12553..13671)
                     /locus_tag="VXD58_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(13580..15049)
                     /locus_tag="VXD58_000429"
     CDS             complement(13580..15049)
                     /locus_tag="VXD58_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV0675178.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            complement(15061..16359)
                     /locus_tag="VXD58_000430"
     CDS             complement(15061..16359)
                     /locus_tag="VXD58_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV0675179.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVVRLLKYSWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDVGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETSRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMNEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            complement(16337..16849)
                     /locus_tag="VXD58_000431"
     CDS             complement(16337..16849)
                     /locus_tag="VXD58_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV0675180.1"
                     /translation="MAFFDCLKGGDGKLNERQRRFADEYIISGNAYQSALRAGYSEKY
                     AKARSSELLDNVGISDYIKNRMEELQDEKILTQKQILVMLSEIASGQAKETIVVTTKV
                     AELMPDPVTGKSVKVYNEIPQLVEYPTKNSDRNKALELLGKRHKMWTDKVEADVSGTV
                     VFANESDIPD"
     gene            complement(17951..18033)
                     /locus_tag="VXD58_000432"
     tRNA            complement(17951..18033)
                     /locus_tag="VXD58_000432"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18000..18002),aa:Tyr,seq:gta)
     gene            complement(18114..18518)
                     /locus_tag="VXD58_000433"
     CDS             complement(18114..18518)
                     /locus_tag="VXD58_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV0675181.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            complement(18590..18955)
                     /locus_tag="VXD58_000434"
     CDS             complement(18590..18955)
                     /locus_tag="VXD58_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675182.1"
                     /translation="MKPKRYPYSGKRQKVSSPLFPARPILNEVPIVEEVKVELGVEAK
                     VGRFYPETIIQLDISGYGNRVVSVHRFPGTLLSVGESIQLKMLFYKKLRNLTTDRFLT
                     FRESDWKFFIRDLVNEFKH"
     gene            complement(18952..19194)
                     /locus_tag="VXD58_000435"
     CDS             complement(18952..19194)
                     /locus_tag="VXD58_000435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675183.1"
                     /translation="MVDKYIDEPHTTFIPVMNGKSSVLVPTRTKRKYILVVSEFTGNK
                     QVEETFEVTVEEYKHYKIGNTFIQDAVLENKEGDKQ"
     gene            complement(19269..19709)
                     /locus_tag="VXD58_000436"
     CDS             complement(19269..19709)
                     /locus_tag="VXD58_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675184.1"
                     /translation="MIPKFRVWVKIGKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYEKDYVG
                     VVKVLEGSWVIDTGSVAVRLWSEIDESEVLGNIYENLEFLEVNE"
     gene            complement(19706..20209)
                     /locus_tag="VXD58_000437"
     CDS             complement(19706..20209)
                     /locus_tag="VXD58_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV0675185.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPQ
                     KVKIPQFVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDGYE
                     VEEEKRYLVTLKNRQPLVKSQSGSTLYFSQDITARNYKGTQKELEEAKFGWVFDCEGI
                     DIEEVEE"
     gene            complement(20211..20528)
                     /locus_tag="VXD58_000438"
     CDS             complement(20211..20528)
                     /locus_tag="VXD58_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675186.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQNLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGN"
     gene            complement(20553..20735)
                     /locus_tag="VXD58_000439"
     CDS             complement(20553..20735)
                     /locus_tag="VXD58_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675187.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            complement(20751..21182)
                     /locus_tag="VXD58_000440"
     CDS             complement(20751..21182)
                     /locus_tag="VXD58_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV0675188.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRTRLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            complement(21179..21508)
                     /locus_tag="VXD58_000441"
     CDS             complement(21179..21508)
                     /locus_tag="VXD58_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675189.1"
                     /translation="MASKINVTERIAIIIEKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            complement(21522..21731)
                     /locus_tag="VXD58_000442"
     CDS             complement(21522..21731)
                     /locus_tag="VXD58_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675190.1"
                     /translation="MEWTDWVDWKPETKTDIKIKIENDGYTFPHYDKKNNGVKYVIST
                     MDIKQDCLRLGVPFEDVYPLQTTLF"
     gene            complement(21733..22428)
                     /locus_tag="VXD58_000443"
     CDS             complement(21733..22428)
                     /locus_tag="VXD58_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV0675191.1"
                     /translation="MEIDKIIKKDVLEFMETIPDNKIDLIVTDPPYLINYKTNWRKEK
                     HKFSNVIKNDNNPELIKEYIKECYRILKDDTAIYIFCSFDKVDFFKKEIEKYFSVKNI
                     IIWRKNNHTAGDLEAQFGKQYEMIILANKGRKKFNGERLTDVWDFKRVSSDELLHQNQ
                     KPIELIKRCIVKHSDVGDTVFDGFMGSGTTALAALETDRHFIGTEIDEYYFGIAEERI
                     KNHNAQLSLFDEV"
     gene            complement(22441..22857)
                     /gene="ssb"
                     /locus_tag="VXD58_000444"
     CDS             complement(22441..22857)
                     /gene="ssb"
                     /locus_tag="VXD58_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0675192.1"
                     /translation="MINNVTLVGRLVAPPDLRKMPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGIIGRIQVRSYEKDGQRRYVTVVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            complement(22952..23287)
                     /locus_tag="VXD58_000445"
     CDS             complement(22952..23287)
                     /locus_tag="VXD58_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV0675193.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            complement(23280..23603)
                     /locus_tag="VXD58_000446"
     CDS             complement(23280..23603)
                     /locus_tag="VXD58_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675194.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFNVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            complement(23606..23767)
                     /locus_tag="VXD58_000447"
     CDS             complement(23606..23767)
                     /locus_tag="VXD58_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675195.1"
                     /translation="MTEIEKISEELAEYGVPDELIGKIEDLLATLYGEKKEIGDRKIL
                     DISPESMGR"
     gene            complement(23770..24810)
                     /locus_tag="VXD58_000448"
     CDS             complement(23770..24810)
                     /locus_tag="VXD58_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV0675196.1"
                     /translation="MQELQVKVTQAQVEIIDREKFEQNINEVVAKYQNYTVTAATIKD
                     DKQTLADLRKLDKQVSDERIRNKKVLSEPADEFDKYVKNAIQPLKDIITKIAGDVKEF
                     EEHQKAVRIDTVKGYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTLKKATMKSL
                     DDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFGQIKADYEFE
                     KQKEEIRQAQERAERESQELLAAQQTKQQEQAPKSTETPNFDPETGKILDGGQILQNE
                     PNALRGAENDLKRYAQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQVRGYQRIEP
                     LTQAELNEQCGW"
     gene            complement(24820..25584)
                     /gene="bet"
                     /locus_tag="VXD58_000449"
     CDS             complement(24820..25584)
                     /gene="bet"
                     /locus_tag="VXD58_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV0675197.1"
                     /translation="MTNNQLATQIKRDITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYQNNSGTEFSLIVSKEAFMKRAERCEGYDGFEAGITVM
                     RNGEMIEIEGSLKLPEDILIGGWAVVYRKDRSHRYKVTVDFNEYVKTDRNGNPRSTWK
                     SMPATMIRKTALVQTLREAFPDELGNMYTDIDGGDTFDAIKDVTPQESREDVVARKMA
                     QIDQFNKEQEANHADPEPTQNEDPIQGELLDGELEY"
     gene            complement(25596..25826)
                     /locus_tag="VXD58_000450"
     CDS             complement(25596..25826)
                     /locus_tag="VXD58_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675198.1"
                     /translation="MTQMTNKGKSFIRAEISEKQKEYIRLLAELRGVTTQELLGQVVE
                     RFIDRNLQLIQDYKNELDDLNSKSRRRIDMNT"
     gene            complement(25946..26089)
                     /locus_tag="VXD58_000451"
     CDS             complement(25946..26089)
                     /locus_tag="VXD58_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675199.1"
                     /translation="MTKIEIENRVWLLANHEEKNELLDLGLTSKARYVKRVLELGKVY
                     AHV"
     gene            complement(26076..26336)
                     /locus_tag="VXD58_000452"
     CDS             complement(26076..26336)
                     /locus_tag="VXD58_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675200.1"
                     /translation="MFKALKTIKKIKQLQKEMHDVSLAFLALQDVGLMPEDERSKAKA
                     QTMHDVSHMLKDVLGGKSVDEAMKRLNSEVKIEEVEQEDDKD"
     gene            complement(26329..26535)
                     /locus_tag="VXD58_000453"
     CDS             complement(26329..26535)
                     /locus_tag="VXD58_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675201.1"
                     /translation="MKSLTKLKLRLEGIIKEVSLDWRVVAVELNEDLLEERKRRFMCE
                     QEIYDLKQQLAIYKEKEQMGEQYV"
     gene            complement(26535..26696)
                     /locus_tag="VXD58_000454"
     CDS             complement(26535..26696)
                     /locus_tag="VXD58_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675202.1"
                     /translation="MKRKKEQWKPRIVNIMADGSVIEDLTGYVIPAGHSYYDIILGMN
                     KRSNKEDVA"
     gene            complement(26914..27768)
                     /locus_tag="VXD58_000455"
     CDS             complement(26914..27768)
                     /locus_tag="VXD58_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0675203.1"
                     /translation="MQVVSSKELQERALQVETLKQQCPKHEGVYMWRSINPCTRNTLT
                     YCPECVQETINQNASEQLAIAEAQIRDTRSYSLFMKESIIPNDLKNATVGNFEIHTNQ
                     DAKAVNFAKRVTADYVKERYEGNTIISGPPGVGKSHLAVGIAKTLNESFQMLQVRKSV
                     VYMPSMELFSRMQEAFQYKDSKWEQRSVVKFLQNVDFLILDDLGKESSVGNEIRQGNN
                     WMQKVLYQILENRTNTIITTNFEGKHLKELYEQSLVDRITKGNMKTNAFKFSKDTASR
                     RSLSATDY"
     gene            complement(27778..28629)
                     /locus_tag="VXD58_000456"
     CDS             complement(27778..28629)
                     /locus_tag="VXD58_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEV0675204.1"
                     /translation="MNEQYFTEIDMDNHERYFKIPYRLLEDDYFSDLDPLAVMVYGIL
                     TDRVSLSRKNKQHFTDKDGYLYVVATNEEIGKWIKKSEPVVIKLKKQLIEHGLLKEKR
                     QGVRLANLLYPQKIRTKEILVQELKNIKGGTKEILVQELKNIKSNQPDNNHPYITNLI
                     EPEGVGANNLYSIEDAPAENDLGIVHDWIFSEFGRYPTPFEIEDLKYFLQDHSKEVIK
                     LAIKECVGNGKPYFKYLESILRDWKQKGLTTVELVENRQKPTRSNNKSNGRLRLSDDG
                     FDPRLGF"
     gene            complement(28626..28853)
                     /locus_tag="VXD58_000457"
     CDS             complement(28626..28853)
                     /locus_tag="VXD58_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675205.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHSLGEV"
     gene            complement(29044..29334)
                     /locus_tag="VXD58_000458"
     CDS             complement(29044..29334)
                     /locus_tag="VXD58_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675206.1"
                     /translation="MTKTIAINTSEHDVLLTARKTHPAVFVDGMFLDGVERVEFTNRF
                     LESCEVVLTFNDRVETNPFPLNDITLLEKLFGKASNGQSLRDIVLQTLEDGN"
     gene            complement(29331..29477)
                     /locus_tag="VXD58_000459"
     CDS             complement(29331..29477)
                     /locus_tag="VXD58_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675207.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLLKQVSHEIELRALES
                     ETKV"
     gene            complement(30068..30343)
                     /locus_tag="VXD58_000460"
     CDS             complement(30068..30343)
                     /locus_tag="VXD58_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675208.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEEIFLYSKLEAFCTNCRQF
                     AFGWTELAEEIECQERYLLDSGFTQDEIDDIRFDAAFVGMQDKMNVA"
     gene            30518..31321
                     /locus_tag="VXD58_000461"
     CDS             30518..31321
                     /locus_tag="VXD58_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675209.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFVTNNSMVDDSSSNTSSIQTIY
                     DQLEPPRQGKVLTFAERQLKEQNKEETKINEVSENIIRLDDYRQTTYRRVTGVVSAGS
                     GSIQDDDLDMEVSFYEDEIPDDYDAIAYVVGNSMEPKIKNGDYLFIKNTQQVDYNTIG
                     IFQVDGANYVKKLRQGYLESLNPDYEDIHLDESNDIRTIGEVVSVYREK"
     gene            31323..31523
                     /locus_tag="VXD58_000462"
     CDS             31323..31523
                     /locus_tag="VXD58_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675210.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            31707..33152
                     /locus_tag="VXD58_000463"
     CDS             31707..33152
                     /locus_tag="VXD58_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0675211.1"
                     /translation="MITTNKVAIYVRVSTTNQVEEGYSIDEQKDKLSSYCDIKDWNVY
                     KVYTDGGFSGSNTDRPALESLIKDAKKRKFDTVLVYKLDRLSRSQKDTLHLIEDVFIK
                     NGIEFLSLQENFDTSTPFGKAMIGLLSVFAQLEREQIKERMQLGKLGRAKSGKSMMWA
                     KTSYGYDYHKETGTVTINPAQALTIKFIFESYLRGRSITKLRDDLNEKYPKHVPWSYR
                     AVRTILDNPVYCGFNQYKGEIYPGNHEPIISKEEYDKTQSELKIRQRTAAENVNPRPF
                     QAKYILSGIAQCGYCGAPLKIMLGVKRKDGSRLKKYECHQRHPRTLRGVTTYNDNKKC
                     DSGFYYKDKLEAYVLKEISKLQDDADYLDKIFSGDNAETIDRESYKKQIEELSKKLSR
                     LNDLYIDDRITLEELQSKSAEFISMRGTLETELENDPALRKNKRKADMRKLLNAEKVF
                     SMDYENQKVLVRRLINKVKVTAEDIVINWKI"
BASE COUNT         8969 a         7615 c         5876 g        10712 t
ORIGIN      
        1 actttggttc taaggccttc agtcagacga ttcacaccag cttcgagcga gttgactcgt
       61 tgtttaaagg tcgattcaac tgttgaaatc tgaccttcta tatcttcagg agcttctgaa
      121 taagaagtat ctacatcgct tatttcaaac ttcggcatcc aaatccaaac ggttccttcc
      181 tggttgaaat tgaacaacca ttcatttgtg gtctgcttgg attcgtttgt ccaacctttt
      241 ggaatatgga caacatatcg tttaatttct gtcgacaatg tcacatttcc agttttatat
      301 ccgatattcc ctaatcgaga tcttagcatt attccatttt tatttgcctt agcataaaaa
      361 ctaatggtta catcttgatt agtcgtactt ccgggaatta ctttcccgaa ttgacccaga
      421 actggataag taaccttggg attccctcca tcacggccag atggattcag acctataatt
      481 ttaagagcgt tatatccaag atacttactt tcgctatcga tagtagccgt atatgtactc
      541 gttgtccaaa ttcctgtttt tgaaatatcc tgcttgaata gtgagttcaa gaatagattt
      601 cgaccggatg cctgcacact cgcaatccga ctagctagct cctcagctgt ctgcgtgagt
      661 tctgacttgc tggctttacc attggccaag ttggtcagtt ctgacagtct acgagtcgtc
      721 gtctcctcat acgtcgcttg cgctgacttc acgccagcca gttcattttt agtccggcta
      781 agtgcttcaa cttgcttggc aatctcagct tcagcctgtg cttgcttcgg tcgaatatcg
      841 tttgcgatag ttcgttttag aacatccaag tcacctgaca gagccgtttg tgcgctcgta
      901 gtctgcgact taaacgcttc aagtctagca acagaatcca gctcaatccg cttagcttcc
      961 tgtgcaagca gggtacttgc gccatccagt tcatttttag tccggctaag tgcttcaact
     1021 tgcttggcaa tctcagcttc agcctgtgct tgcttcggtc gaatatcatt cgcgatagtc
     1081 cgtttcagag cgtccaagtc acccgacaga gccgtttgtg cgctcgtagt ctgcgactta
     1141 aacgcttcaa gtctagcaac agaatccagc ccaatccgct tggattcctg agcaagagaa
     1201 ctacttgcgc cagcatttcg caaagcttcc tcagccttgc gcttagcttc tttcaatggc
     1261 ccgttgtcaa agctattaaa gcgctgattg atagtgtcag acagttctct cttgacttct
     1321 tcagctctgg ctttggccag ttctacttga tcgttaaagt cttttttgat tttgtcgacc
     1381 ttttggtcaa aatctttatc tgctgcttca atctgcgctt ggattttcgc ttcaaagcca
     1441 tcttgttgct taatctgctt ggtaatcgta ccctcgtaag aatactgggt atcgtttcca
     1501 gccttactat ctgcactgat acgacctctc agaccacctt taaagataaa gctctgactt
     1561 aagacaggaa ctttaaaagt ctctttcttg ttggtctgaa tggttaccca ctgcccaacc
     1621 tcaagtaaca aatgtccttg gtagttgaga ttatacggat agtaagttag gtttttcagt
     1681 ttgtaataca agtcatttaa agcgctctgg gttatgaaga cattatcaag ttccaaagac
     1741 cgacctgttg tcataccgac cgtcagagac ttcttatccg tcttacaagt gataccagct
     1801 atctgatact cgatttcact cttggtcaat ccatgcaaga agtaactgtc agcgttaatc
     1861 gtgatgttgg actcggtcaa atcacggatt tccatctttc cttctcggtt gaagaagcaa
     1921 gacatcccaa tcatctgagt catagcgctc agcatatccc tgaacgaaag tttcttacct
     1981 tcaggaactt gctcgatatg gtaacgcata gcgctgattc cgaaatagtc attcgctaac
     2041 tcaatgcctg ttttcaggca gatttcctga ataacctctc gtacttcagc tgggaaatgc
     2101 aaatccgtca cgtactcacg attgagctta aacataccgt ccataagttc aagtgtggtt
     2161 gtgttgcggt ttcggtcaat ctcaatatcg ttgatgaagt attcccccat cttgacccac
     2221 tggtaggtat ccccaaccag tagaccaatc tcagggtgca gggtatccag cttattgaac
     2281 gtggtaatga tactggtaaa ggtaatttta ccgctaccag cgcaggttcc accaggctta
     2341 taagtatcgc ccttgatgta gccatactca aaactagcct ctttgatatc ccgtgaagca
     2401 tattcaccaa cacgaatagc cagcgtcctt tccttggcaa acatggctct gtcaaattgt
     2461 cgtctagtta aagcgtccat tttcttacct ctctaccaga ttaaatttag cgccagacca
     2521 aggtttaagc ttctcagtaa aggtatagct aggagctgtc ctatcaccga catagaaagt
     2581 ctttgtgact tggccatcca tggggtctgg ataagatacc tcaaaaaatt tagatgatac
     2641 agcatgtaaa agctgactta tttctccctg agtcatcata ccccattcac agtctagttt
     2701 gcgtttggtc gtgatacggt cacgcaccat gtcgccattg gcattacgcc ctgtctctcc
     2761 atcgatatct tgaataccga cttgaaaaga tttgggaggc ttcacagcca ccccattgat
     2821 tgtcaattgt gccatttaac ctcctaaatc ttgagcaagg tttgacctgc tcgttcatgt
     2881 tccttgttga tttcttggat agctacccgt ccgaactcat ggcctgcgat ttgaataacg
     2941 atgtcgccgt cgctagaaaa accaccttgt ggactaatac cagccatggc atttactacc
     3001 gcactgctga ctactcgtcc aagtgtttgg ataaatcctg tattttcaag tggtacgacc
     3061 gcctctttac cagcttcacc aatcatggcg attgttggac tatcgacgat accaccacgg
     3121 gcaagacgag ggaggctaac tgtgcttaca ctaccaaccc atcctagacc aggtaagttt
     3181 ctgacaacgc ctaaaactcc attaatcatt ccgatgaagc cattgactac attttcaatc
     3241 gttccaagaa ccgcattgac cgcactctta aacgctccac ctacagcgct accaaccttt
     3301 tgtccagcat tgacaaatat gctttgaacc gttgaccata caccgctgaa gaagccacca
     3361 attgaactaa acgcgttctt gactgcatta taagcactag taaacatatt cccaaaccaa
     3421 gaagatacat tcgcaagaac acttgtaaca tctgcccatc tctcgccaaa ccataaacct
     3481 agtttgctaa agatgtttgt tagaccagtc catgcttttt ggaatatgtc agtaaaccat
     3541 gccccgatat tagccaaagc actagtcaca tctgcccaac gttgtccaaa ccatgagccg
     3601 attggagtga agatattaac gatagcgtcc catgcacctt ggaatacacc agagaaccac
     3661 tctccgatac cagagaagat gttgacaatg gcatcccatg cttgctggaa cttctcgcca
     3721 aaccattgac ctatcggctc aaagatttct tgtagttttg tccatagacc actgaaaaat
     3781 tcgccaatag ctcgacaaat accactaata aaatcacata gtccttgcca tgcagtttta
     3841 gcaaactcaa caacagtgtc ccagttttgg tagagcaaga caccgatagc aatcaaagct
     3901 gcgattgctg caataatcca tgttattgga cttgtcaaaa ctgctaacgc tgcattaaaa
     3961 gcccatgttg cagctgtagc gactcctgct gcaacagaat gtgcaaattc cgccgcggtt
     4021 gctaatccca ttttcgctgc atgagcagtc catgctagag ctgatttacc aagttctaaa
     4081 gcagtttttc ctagctgtgc aattgtttta cctgaattga ccacaaaatc ttttgcatat
     4141 aaggtgttca aatagattgt ttcaccaaaa ctgaccaact taccaaatgt caatgcttta
     4201 atagcaagac ctagattctt aatccctcca acaatcaaag agaccttact acctaacaag
     4261 ctgaatgctc ctgcaagtcc cccggcttgt tcagcccatg ataagaaatt aatcgtttgc
     4321 caagttgtta tcaaagccaa gataggttct ttgttttctt tacaccagtc agaaaaaacg
     4381 gtgaaaccat ctgccactaa cttaatagca tccgccaata gtcccaaagt ggctaaaagg
     4441 ccacctccta ataaatctga aattccttca atactaacac cgaatactcc tgataaaaac
     4501 tcagcaaaag gttgccaaga attctcccag aggatttgta tgatatcaat cagtccgtta
     4561 aaagcattag caatagagtt aatagcaggg gctacatgtt catcataaac acgacttaag
     4621 ccatcgccaa atttgttaac agacctttca atgctctcaa atacaggcgc aacagtatct
     4681 aataaacttt ggaagactga tgaaatttta ggagcgcttg tcacaacgac tttttcaaaa
     4741 cctttaaaca aacttcctgc taatttacta ccaacttcaa caatggtaga tgtcaaactc
     4801 aacagagttg acacaatagc gctaccgata cgaaccgcac cagttgaggt aatgacgtcg
     4861 tagaaagtac tagaaaagtc ctgagcgatg tttcctactg cctcggaaat gttaccaata
     4921 ttatcaaaca aagcgactag cgccctggta atgcgttctt tttgccttcc aaggccattt
     4981 gcaatacttt cggcaaggaa aacaccgata cctagcccga tagttgttat tgagcctgtc
     5041 acttgcccta aagcataagc aattttctca gccattcggt taaaggcatt cacaaccctt
     5101 gggtcagtgg cgatttctcc cattgtctta gctatttggt ctaaggcagt cttaatgcgt
     5161 tctatacctt ctggtctaaa tgctgcatca aaacctttct tgaagaggtc aaacaaccct
     5221 ttgagcttat ctccaagacc atcaaaaatg ctcttgaatt tgttgtccat gtcggtcaac
     5281 tcgacttctg gcaagatgtc tttgaaaggt ccgccaccgc ctccctttcc tttaccacct
     5341 ttgccaccgc ctccagaccc gcctgcgtcg tcatcttttg gtttttgcaa gatgttaatc
     5401 tcatcaaatc ccaaaagacc tagcaactct ttagcggcct tcttagcgtt tttggcggag
     5461 tctccaagat tgtcagcaag tcctcctgct gaatctccag cgtcgtctac tgcgtcagca
     5521 aggtctcctg ctccgcctgc agcatccttc atggcgttac ccatgtctcc aactgctcca
     5581 ccaacaccat ctttcactgt tgctttcttg ttgaacatca aagcgataaa ctcagcgagt
     5641 ttagcagtaa cgttctttaa gaccatagca aaagagttca agacaggcat aatggcattg
     5701 ataatcggta acatagagtt accaaggttc aatgctgcgt ccttcatcag cgacttaaat
     5761 aggctgatac gaccatttac agaattagac aaggtatccc catacttggc tgtagcctgt
     5821 tccagaatag ccataaggcg gatttgttgc tgggtttggt aatccaactg ttgccagctc
     5881 tgtccgtttg cgaacttctt aaaggcttca gtggactcaa tcatagccac gttgacgttg
     5941 attcctaggt cctcaatcgc ttcggtgttc cctagtaaac ctgagcgaat ccgctccata
     6001 acgtctgtaa tcgtgcgccc tgaaccttca gcaacaactg ccgatgtctg caacatctta
     6061 gcggtatagg cgcttagctt gttggtatct ttgataaatc cagaaaataa gtttgagtag
     6121 actgcaccgt agttggtagc ctcacccaca cccatattca tagcgttggc attatcgtta
     6181 acccatttta agaaagattg cgaactctcg cccatctgtc gcttgatttg gttcatagac
     6241 gctgatactt caagagccgt ctgcgttgaa tacatcccaa catcaagtaa tttcttacca
     6301 aggattgcaa aaccagcgaa cttagccagc ttgccaaacg cactaccgat tgagctcgac
     6361 tgttcacgaa ctttggcagt agcatttttc acttggtcag atgttccttt gacctgattc
     6421 tcgacttctt tcatcttctt cctgaaaggc gctatctcag cgtcaatcat gactttcaat
     6481 tcatcaagag ttgccattta cttcctcctt cctttttcga ttatgtctct ctgcaaattc
     6541 acgcatccgt tccttatgca acaaaagtgc ttgtttctgt cgttcctgtt ctactgcttg
     6601 ttgttcttct acaaacaact caggcgcata ttcccagaac tcaaaggcct tggcatcttt
     6661 ggataacaat aaagaaacgt ggttggatat catctgcgaa agtctgtatg agtcaataat
     6721 cttttcttta cgctcttgga ttttgacacg gttatagctt tcaatcattt ctctgatttc
     6781 aagtaccgtc aaatcccaaa aatcaagagg cttgcccccg atgtccaaaa acataggata
     6841 aagcctctca ataatctgag ttaccgttaa gattacttgt ctactgtcat tttcttcttg
     6901 gaagttttct tgtccttgct tcctcgtgga gtaaaacccg atacttcaaa gagtggcatc
     6961 aacacctctg tcatgaaggt tgtttggtct ccgccattgt ccacgtattc atcgtataaa
     7021 tcatagacat cctcaagaga atacccatgt tcatactgct gcaaggctcc gtgaactaac
     7081 aacaacataa ctttcaaagg cggtaaagtg aactcttcgc cagcttcagg catgaaaatc
     7141 ttcagcaagt tcatgccgat tttttcttcc acagttgcag cttgatgaga tgtcaaacgt
     7201 agcttcaact ctttttcgtc agtaactttc caagttgtgt attttaacgc catttaatta
     7261 acctccaata ccatcagtaa atgtaagatt agactgcaag gcaatcttaa gtgtgaaatc
     7321 gataacggca ttgacaccgc caccgccaag cttaacagat acttggcctt caaaaatgac
     7381 cttagtaccg tctggataag cttgttcaaa gtagagtttc ttcttgtcgt ctgctgcctt
     7441 acgcaataca cgataaggag cagttgcgct tgaattatta taagagaact tgtactcaag
     7501 ttctcctatg tccccaatac caaactcgta cttcttcacc gtatcttcaa gagtagtatt
     7561 ttctactttt tcgagttcaa taccaaactc tggtacttct ttcaatccaa caagtttagt
     7621 atagctatct tttgtttcgc cataagaaag cgtaattcca tttgctaaca tgtttaattc
     7681 tccattctaa attgaaaaac aagctctgag tgtaagtcga cgataccttc aaaacgcatg
     7741 accttatgtc tcaaatgaga cgggtctggc acgtcttggc agtcagttct tcgcaaacct
     7801 aaagactcaa aaatctgatt gattttaaca gctaactcac tagtgctagt atcatcaaag
     7861 atatccacct tgtagcggat agaggatttt tgttcctggt catcaaacca atcacccggc
     7921 ttgttttgtt cttccaaaaa aataacgact gggaaagtct cccaatcgct aggataagta
     7981 tcggtcacat tatctgcaac cttttgcaat tctttataaa taacaggctt gatattaatc
     8041 attttatttg ttctcttatc tttctacgga cataattcga aatattctta gacacacgct
     8101 cttgattgtc tctcaaagct ggataaagat aaggctgggc aggttgacca tacatcttgt
     8161 agaactcccc gattttttga aagtggtacg gtcctacatt gatttggtct tcatgtacat
     8221 accacggact agacttgtaa gtcacgctga cctctggaga aataccagaa tggctagctt
     8281 gtcctattgg tcccgttcca aactcaacat aaggagcgta tttaaggttg gtgtaaactt
     8341 cgcctatagc cttatctccg tccatttttg ccctagtttt gatactagtt ataagctctc
     8401 catctctcgc tggtgcgagt cttcttgcat ctgcttggac aacctttata gtagcattgt
     8461 gcaccgcacg taagacgata tcctcgccag tttttttact agccaatcgt ctacatttag
     8521 ctataagcct atctgcccct agtagccctg acacgctcta actccaaaac ttgatgatgt
     8581 gtgtagacct ttttagaaat aaccctatga gtcacttccg tctgactatc gatacacaca
     8641 ccatctttta ctttgatagt agctgacttg ttggcatttg cgttcaaaat atcattgaca
     8701 cgctcgccat acaattcaga ttgtagcttg ctactagctg gccacaattc aaggcggact
     8761 gtctcagctt ccttggcata cccttctttc gcgacacctt cctcagtgac agtcttttca
     8821 aaccgtcgca ttggataagg tttcagtcta ctctgcttca aaaacatgac ctgccaccct
     8881 tgctagcctg tgcatgcgta ttcgctgtag aagatccgta gacaggccgt tttctccgta
     8941 gactactgct ataccaccct cggttctaga atgctctcct tccgctcctg agcggttgtg
     9001 gagttcgata gcaacctcag gtattaaaag acttaaagca ggtgtcaaag atgtgcgatt
     9061 agtctctgac aagataagat ttgtagccct tgtttggagc aacatgagaa gctgagtatc
     9121 ttcttcgcct gttaatttct tcagcaactc tatagacata tcaatcctct tctaagaact
     9181 caggtttagg aaagatttcc tcaagaacat ctgagatagc gacaccgttg ctggcaatat
     9241 tgtcagccag ctcagcatag cgctcctcag taatctcaag ttcctctcct gccagtcgtt
     9301 tcacatttga ttcccaatca tagaaatctt gtttgatttt aaatttcata actcggacct
     9361 atttcttacc agttttttct ttccagttag ttgtatctgt gtctggtgcg gttgatgaat
     9421 tagaaatatc cttaaccgct acatagactt tatcggcatg agtaactgtg tcaccttctt
     9481 tgtaggttgt tccagttttc cacgctttag cacggtttac aactttacct tgagtagatg
     9541 gtttagcagc aggcttagaa tctgcaattg tgatgatgta tttcttgaag tgttcaagaa
     9601 caaatgcacc agtgtaaagc aattgctcta ccaattcgcc aaatcgccct ggaatgttat
     9661 cgttgtactt agtattatct acttgtactg gagatgtaac aacacctgga gcagtagcaa
     9721 gggcattaac acctttcagg aatttagaag gaaccttata gactgtgtaa tcatccaatt
     9781 caccaacata tccttttcca aggactttct tatctgcgtc accatgtggt agacgaacga
     9841 tttcagactt gatcgctttg tagaaacttg gagtgacgaa gagcaagcgt tcttttgtaa
     9901 ttccaagctc atcaagtttc tcagacacat caagaaccgc attataagcg ttgttcgctc
     9961 ctgctgtttt acccatggca acattgtcac ttacgtttcc aagtgctgca ccaaaacgta
    10021 gttcatcaag atatggagcg actacttctg cagcctgacg ggcaataaca taattgatat
    10081 tcacttgacc attagagtca cgttcgtcca attgatctac gaaacgaccc cagtattttt
    10141 cttcatcaag ggtataaacc ttttcttcaa cttcaacgtg atcaaattcg ttgtctttgt
    10201 tacgtttgta gtctttcaac tctgttgtgt taccagttgc tactgtaaaa gagcgacctt
    10261 gcaaggttac tgcatcgctt gatgtcacaa gtggtgttga atatgaattt actgcaagta
    10321 catcctcaat aatcccaaga tgtttcttgc gtgattctgc tgtgtttaat tcttcaaatg
    10381 ccatttattt ttcctctttt cttttattac aagaagtctt tacgccattt ttccgtgact
    10441 tcttgctgga ctgtttgtgc atttttgata ggtgcactac ctttcatacg ttcagaaact
    10501 cccttctgaa ctgactcttc ccatgctttt tgtatagagg taatagattc agataccgtc
    10561 tctgcgcttg tcaaatcaac tacatttact aactcaacag gtaagtcacg ttcacttagc
    10621 attgctttag cttctgcggt caattccttg cgagcaatag ccttctcacg gtcagctagt
    10681 tcttgctcac gctgatccaa ctgatatttc tgtttctcgt cagcgttcat cttggcaagt
    10741 ttcttggctt cgttttcctt ggcttcttgc tcagcttccc atttagagcg ttcggcagat
    10801 agcatcttac cgatttcagc acgagtgaaa gttcgttcgt gcttttcttc ctgcactgaa
    10861 tcaacatttc cttgagtgtc gacagtctca gttgattcag tagatacagt tgcattgatt
    10921 tcttctgaca taattgtcct ccagcgatta cgtcgccact cgataatctc gctttacgtc
    10981 cggcgacgga acagtacagc ttttaatgtc atcggtacag tttggacaat ataaaaaccg
    11041 tacgggattc catacggtta gattattttg tattttcttc aatcactttt tttacaaatg
    11101 ctatgataat cagcatcaaa aacaaaaata ccaaccaccc gaaagcgatt gatacccaat
    11161 cccatataaa catatcttac tccttttaat gtcacaacca atcaacttca tacgataatg
    11221 aatgaatgtc ggttaatatt ttaggtagta actcaatcgc gctgaacgta tccgccccat
    11281 aaatatttaa ctctaatttc actgtcgctg attcaattcc gcctgttcct gaaaattcta
    11341 cgttagttat cccaattttt gctttatcca ttttcaatcc tttctgggta cgaaaaaagc
    11401 acttagattt ctctaggtgc ttaagtaata aattgcattt ttatattttt taacacgctc
    11461 gtagtctgta ttggtaacag atttcaaacg tgataaatct gagttatgtt tcaaatctgc
    11521 aagttttaca actcttgcta aattatttga ttttactttc ccaagatatt cttgataact
    11581 ttgacctttt ttctttgtca aaatttgtac cgctgtaaca acttcatttg acaagccaga
    11641 cgcgaataaa tcggcagcag ttatatcgct atcctcaatc acatcatgta aaagagcgac
    11701 agctttttct tgttcagtgt tgacttgact ggccacatag agaggatgct gtatgtaatc
    11761 aacatccgct ttatctacct gccctgcatg tgctttttta gcgatagcca aagcaatatc
    11821 aatcatgccg ctaccatcct gtcaatataa gtaaatgcat cattttctga aatttcttca
    11881 aaatccgtaa agtcattaaa aaagatttta ttaaaccaat ctatgctatt aacccacttt
    11941 ttttcaatgt caaaaacttg catgacacca tcaatcaaac gaagtacttg agcattgttc
    12001 gtcgttgtgc ggtagtattt aatatctttc acatcacttc accctctcta tatttttagg
    12061 aatctcaagc ccattgctta aatcaagcat ttccttaaat aatttcatgc gttctagatc
    12121 agatgtattc gtatcacgat acttctcata gagttcatgt aatgaaccat tttttaagtc
    12181 gaaactttcc tgagtatgat actgcatttc aaagttgata ccatcttttt caacgactgt
    12241 attcacacct ttgtatggtc catctactgg ccaagtgttt tttactttta caattttata
    12301 accttctgcg ataagcttct gtttcatctt caaatactct tctgtaaaag tatcggaatc
    12361 gaaaatagtt gtgtacctta aggcgtcatt gatcttactc acggcttttg acaaacttat
    12421 attttcaact aggctatcta caataatttt acgtgataat gactcaactg ttttcttcct
    12481 aaattcaaga cctgccaatt tgttttctcc tgcgatacgt tgcatatcac ttgtaatttt
    12541 tggctcaact cctgaaattt tggacaatag ttgttcgcta taaaatttcg ccttggcttc
    12601 tcttgtatct tgattataca ccttttcccc gtctttcgca acatacttgc tataccactc
    12661 tttataagtc atatcagcag gtactagctc ggtcttccct gtctctggat tccttgctct
    12721 gcgcttcaac ttgctgtaat ctgcgtcctc atcgtatccg acagtagtag acctacacca
    12781 agggtgcata ggcggacaat tgacgccagg gacagccttg tccctatcat agacctgatt
    12841 gtcatgctcc tgacaaatcc gtgatgtacg cttgtctaag acggccgcaa agatatactt
    12901 ttctatgtct gcttcttcat agctgagtag ttccatttgg ttatgaaaaa aggctgattc
    12961 tgtccgaacc aaacgtcttg catcgttctg acctacattg aacctctcag caattgcttg
    13021 tgcagtttct cgtgtatctc ggcctgtcat gaggctcata agtagttcat cttttatgct
    13081 agaagtaagc ttccccgtat tcttccagat gtctgtagag taggtacttc cgtcacctac
    13141 ccaactgaaa gactgtagat gtttaatctc gctctcagga agcccagaaa agccgtatgc
    13201 tagtcctgtc tgctgctgca ggtcaaaggt agccttgtag taactatcct tcatcaagtc
    13261 gctataaaag gcgtctgagc ctgtcttctc tgaatgatag atagattcac gcatacggtc
    13321 taaatcgtcg ctcaaacgct ctaggcgctt catacggaaa gaataagctg ggctgtctaa
    13381 gtcagctagt aatctttgga tgttcgggtc attcggtctc gcttcaagta ctttacgaag
    13441 ttcattcaga tttttcttgt ctttcatgtt cttcaatact tgtctagctt ctacctgact
    13501 tagaccataa tcacgttgga acttatcaaa aatcttattg acttccttat ccaagtaagt
    13561 cttggcttcc tgatagacct tatcgaactg gtctgcctgc ttttcggcct tgtccatctg
    13621 ctggtaaatc agattggctt tcctcttcgc ccaatactcc tgattcttca tcctctacct
    13681 cgtcttcggg tttcgtgttg tctttgttga acatcggcat gtcttccatg ttcttctttt
    13741 tctcttcttc caaggcttcc agctcagcgt cagggtcttc cacaaacggc aagagagaaa
    13801 taagctgcct attggttact ttgccttcca agttgttcac aatctgagag atttctaaca
    13861 agttcttagg caaaccacga ctgaattgtg gaacgattga atgagactct aaagcaatct
    13921 gcttcatgcc taagtaatga gcaaaaatcg caatacgctg acgcaatcct cgcttatagt
    13981 tcgcttcctt ggtcttagta atcatctcaa ggcccatcag cttaaattcc atggctacgc
    14041 ctgatgtatt ccctgcgaaa ttctcatcag tcaaattagg cacatggcta aatgtgtaga
    14101 tgtcctcttt aagagctgta cgcaagattt cagtagcact ttcgtccagc gtattcttca
    14161 agaactcagc ccttgcacta tcgcccggca attccaaaag accttcttca gaaagaatct
    14221 tcattgctac cttagcgtct tctggagtgt ctgctaactg cgtgccatac aagacaagta
    14281 tagactctac agcctgttcc ttatcattga cacgattccc catcaaggaa ttataagcgt
    14341 ctatcaagct aatttgttgc tcatagtcac caattgcaaa gtgattgttg cgatattcga
    14401 taattgggat ttgaccaagg ttgtgaggtg ttgcctcctc gctctgagtt gttcctgaat
    14461 ctgtacttct cagcaccatg tgatagtgca gattttcggt aaagacctca gcctggtgct
    14521 tggtagtgtc tttcgtatcg tcttttactt catagtaata gaccgcaaac aaaggctttc
    14581 gctcaatact atcatcgtag accatgaaag tattctccgg atcaatacta gttgaatcca
    14641 actcagtcat accctcttta gcatagatgt actcgtaagc acgaccatag atagccatgt
    14701 tcaaagcatt ctgagcatct acttggtcaa tctcagcacc atcaaaggct gtaagtagtt
    14761 catcgatatc accgtcagca gtattgttat acttgatagg attgcccata aaatagcccg
    14821 tagccgtgtc tgcgatatcc ttggcatgat tggctaccgt cttgtaatta ggtgcgttca
    14881 cgttgcgtct cgtgtgttct aagatagcat gctcacccaa atagtagctt ttaagcttct
    14941 tcaaatgcga gccttcagtg ctatgtatcg ttatcaattt gtaaatcagg tctttcttca
    15001 aagaaccctc atcatatcca tcccgtggat aggttaaata ttggtacatg tctttcctct
    15061 ctatagacca taatcagaac gtctgcggac ggttgctttt ccaccttcga tacattgaag
    15121 gctgtaacgc aaagcgtcca tcaagtggtt gtttttatcc tctggtttat tcaaccagtt
    15181 gccttcttta tctcgctggt agcagtaact ataaaattca ttcatgatgt ttttacaatc
    15241 cggatgcaca taaatagcgt atccttgcaa tttggatacg cctgccataa tactatcctt
    15301 acctttccga ctctctttta ttctagatat gccatgttct gacctgagct cttcaatcag
    15361 ccgtgactct gcgctatcag caatgatttg tgaacgatga taacctttgt cttttatcat
    15421 cttcgcaact tcttttgtta tcaacccaac tttatacgcc tcatcaaaga cataaatctc
    15481 tttcgtcgtg tcatttatca acgaacaaca caaagcggtt ggatcgtgag taaaaccaaa
    15541 gtcaagaccg atacataact tattagctga atctcgtagc aattcatcct tatcgaaatc
    15601 cttgacggtc acgttctcat agattaaacc ttcagcaact ccccattcac catcacaaac
    15661 gattctcgca cgccttggat ttgtgtgata caagtcctca tagcgcttaa tatcgacttc
    15721 atcaagccac tcattgcatt tgtaagtggt tgtagtagcg aatgtatcag ctcggctagt
    15781 ctcttcatca aagaacacac gtttgagcca gtgcctctca ttccacgggt taaatgtgac
    15841 tgtgatttgt ttaaagaaat caggtacatc taagctacca cggattgact caactactgt
    15901 actgaacttg tcttcagtct caatttgata tgcttcctca aaccatgccc aacaaagact
    15961 gccgacatcg accgtgatag atgtgatttt gagttcatca tccaaaccac ggaataggat
    16021 tttttgacca gtcgctttta cagttatttc aggcaaagac tcgttaaatt taaacaaatg
    16081 agtcacaccc aacacattac acgcccattt aaaatccgta taagttgatt gcttatttgt
    16141 attcgaatac ctacgaataa caagcaagtt ggcccaggaa tatttcaaaa gacggacaac
    16201 atagtttaaa gcggttgtct tggacttctt cgaaccacgg gaccctttga ctacacgata
    16261 aagacttctt gagcgccaga actgtccgta cccagctcct actgtcttag gtaggtcgat
    16321 tacaatatca ctttgcttaa tctggtatgt ctgactcatt tgcaaacacc accgttccag
    16381 aaacgtctgc ctctactttg tctgtccaca tcttatgtcg tttacctaac aattcaagag
    16441 ctttattcct atcgctgttc tttgttgggt attcgacaag ttgagggatt tcattgtaga
    16501 cttttacaga cttaccagtc acgggatcag gcatcaactc agctactttt gttgtgacta
    16561 ctattgtttc tttcgcttgt cccgacgcaa tttctgatag catcacaagt atttgttttt
    16621 gagttaagat tttttcatct tgcaactcct ccattcgatt tttgatgtaa tcagaaattc
    16681 cgacattatc caacaattca gaagatcttg ctttggcata tttctcacta tatcctgctc
    16741 ttaaagctga ttgataagca ttacctgaga tgatgtactc atctgcaaaa cgcctttgtc
    16801 tttcattcaa ttttccatca ccacctttca aacaatcaaa aaaagccaca cgatgtgcga
    16861 ccttttcaag acctctctct gcgaattaaa atcgcaattg gaacgacagg attcgaacct
    16921 gcctacgttt caattcccta aacaggactt aatccgtcta ccatgtatcc attaactagc
    16981 atgagactac tgctttaaac gagtgacttt tgataactta tagtttatta tcttgtccac
    17041 aaatattcct acttgtatca ctcatgcacg attggttaga ccaatcactc ctcacatcgc
    17101 aaactactaa gccatttttc aattaacgaa gactccgcta aaagtctaag ctgctttact
    17161 ctttgacttt actctcatcc ttgcgagact tgagcaggca atctaattgc cgaagtgcac
    17221 tttcgtttac gacgggcgat gacttttgct tttttgagtt ttttctatct tgaatagcct
    17281 taaaatataa aaatcatctt tcatctatca cagacacgca tcgccatgtg tttcattctc
    17341 ttttgaagaa caaaatgcac agcgcctgct tgttatcgat tgttttgcgg acaatcgact
    17401 catcttacat acttttggga ggcacccaat ttttgtaaga tatggtatca agctcttgtt
    17461 gcacctcgaa ccaaatacct ctttcctctt atagactcgt ttcacagcca aacagccaca
    17521 tttgcatttc ctcagcacct tgccgttgga atcttcctgc tttaacttcg cctacctatt
    17581 ccaaaactga aatagttaag attacattgc ttagattgac cattgctggc aggatgtttg
    17641 atagatttaa aaacatcctt ttcctgagtt accacagatt atctaggcta agccctaaaa
    17701 gtgcaaggcg actacaacct tgcgtgcgta ttaaattttg acttcttttt tattttttgt
    17761 agtctttaaa acctctgagg gaatcaaacc ctctagctta taacttatcc ggaatataat
    17821 tagctacgca atcatgcgag gtccagtcgc ttccgcaacc atttttaagt taatgagtga
    17881 taggagttaa tgagtgatat gtgaatcccc acccagaaga tttaactcat tctgggacac
    17941 aaacactcaa aggagagggg aggacttgaa cctccaaggc cattacagcc ccctgacatt
    18001 acaggtaacc atctaccaat tctgagacct ctcttttcaa ttcttgatac taccattcta
    18061 acagattttt agaaccgtgc tgttccaaaa agtcccataa gctcactatg aggttagatg
    18121 acttcttcca aagctaagac cgcctcattt tttaacctgt agtaggttgt acgactcata
    18181 ttcaaatcat aacaaacgct atcagcggtg cctttgttga tgtaagtcat tcttaatact
    18241 gccctgtact ttggattttt aagcctattg atcattctac ctaattcaag ttttctgtta
    18301 atgacctctt tagtatcctg ctctatagcc tctttcatca ctaccaactg agtatagaca
    18361 tcatcaactt ttctagtctg tccaccttgg actttgacgt caatccactt ggggcttgag
    18421 agcaaacctg cctcaagctc gttaatttcg tctatacggc tttggatgtc catgtccaga
    18481 tcctgcaact ctttcaagag ctctttagcc ttgttcactc tctgtctcct ttgtggtata
    18541 ataatagtgt ttgaaattat tgctgagaca gagaatgtct tggctttttt taatgcttaa
    18601 attcgttgac caggtcacgg ataaagaact tccagtcaga ttctctaaaa gtcaagaaac
    18661 gatctgtagt aagatttcta agttttttat agaaaagcat ctttagttga attgactcac
    18721 cgacactcag taaggtacca ggaaagcgat gtactgaaac cactctattc ccgtatccag
    18781 aaatatctaa ttgtattatt gtttctggat aaaaacgccc aactttagct tcaactccga
    18841 gctcaacttt gacctcttcc acaattggaa cttcgtttaa aattggtcgt gcaggaaata
    18901 atggcgacga gactttttgc ctttttcctg aatatgggta tcttttaggt ttcattgttt
    18961 atccccttct ttattctcta aaacggcatc ttgtataaaa gtattaccga ttttatagtg
    19021 cttgtattcc tcaactgtta cttcaaatgt ttcttcaact tgcttattac ctgtaaattc
    19081 tgaaacgacc agaatgtatt ttcttttggt tctggttggc acaagtaccg aacttttacc
    19141 attcataaca ggtatgaacg ttgtgtgagg ttcatcaatg tacttatcta ccactgtccc
    19201 actcgaaatc tggtgacatg ctacgagtaa ggatgcgaat aaaacaatac ataggatttt
    19261 aaaatatctc actcgttgac ctccaaaaac tctagatttt cgtagatgtt gccaagcact
    19321 tcagattcat caatttcaga ccacaaacgc actgcgacac tgccagtatc aataacccaa
    19381 gacccttcaa gaacctttac aactcctaca taatctttct catattcata gaaaccgcca
    19441 atttcgtcag ctctacccaa aaatctagta gttcgtacaa tatcgccttc aaagatttcc
    19501 tttccgttct tgtctttgag tcctgttgat tgcatgagtt cgatttcgtc aaaatcataa
    19561 caatagatat ctctatcgtc tggtaaacca ttctcaaaat aaacttgttg tgtcactatt
    19621 tctttgtttt cgtagtcaat agcaagaatg tcatctgaaa aaaccatacg ttttcctatt
    19681 tttacccaca ctctaaattt tggaatcatt cctccacctc ctctatatca attccttcac
    19741 aatcaaacac ccagccgaac tttgcttctt ctagttcttt ttgagtacct ttataattcc
    19801 tagctgttat atcttgacta aaataaagag tactccctga ttgcgatttg accaaaggct
    19861 gcctattttt taaagtcacc aaataccgct tctcttcctc gacctcgtag ccgtcaagcc
    19921 aagcaagaca gaatttttcg atgttatttt cgtaaaacca atcaggaact ttcttatcat
    19981 aatgatcttc aattactctc attgcaccgt aaacatgaaa attgtttttc tttttaaatt
    20041 ctatatattc cgccacaaac tgcggaattt tgactttttg gggttcgtct agttgtttca
    20101 actctttcaa aaaaagttca accattgaag tataaggtac aggttcataa aaagggctgt
    20161 gttcattcca caattcttca tacttcttaa tcaatgcctt aatattcatc ttagtttcct
    20221 ctataaatca aataaactgc aataactacc tgagccatgc ttggcgaata gccaacccaa
    20281 tcatcaaact ccttagattt tggcaaccaa tccttagtag ctcccaaatc atagtctgta
    20341 ggcttttcat cagcgaagat gcattccatc gctcccataa acgtcatacc atcttctgcc
    20401 atttcccaaa aatagtccgc ccggtctttc accgcttgtg gtaaattttg cttgggaggt
    20461 tgcggcttcc cgtcttctac cgtccagttg tatacttcat taactttttg ctttaactct
    20521 tccatcatct tccaactcct ccgctttccg tcttaattct cattatcttc ctcctcaatt
    20581 ttaataacgg ccctaccgtt tgggtgtcgt ctttggtgtg atgtgtaagt gtaatacttt
    20641 aacatccttt cagtaattcc tgtctcgcta ctgatctgcg ctaatgttcc aagcgtaaca
    20701 aacacatcac cttgatataa tgcgtagtca gccatctgct cctcatttct tcaaatactc
    20761 agggatttca tgacctcaat ctcaacctct atccgtggat ttagactgta gaacttgcct
    20821 acatcatgta gcgctatctg accgtcgtcc tggaagacga tccctgacat gctatcatat
    20881 agcgcttttt cgtagttatc tatgtcaggc tttttgccta ctggtataat ttcatccagg
    20941 agggcctgtt ggttcttctt gactttagaa atgtactgag gaggtttgat ataaaatcta
    21001 agccgtgtcc tcaaagctcc ctcaaggata ggctgaccca tgtactgatt agcaatgagc
    21061 agctggcaat gattacgcca tgttttcata tccttgtctt cgtaagttgt ggtaaaactc
    21121 ccacgtcttg caaaccttgg ccgtgattga ggtttaggct caatgttcag ggtcaatttc
    21181 attcaagagc ccccttaaat cctgccatct caaagagatt ttctctgttt tcgtttacga
    21241 actcaaagaa ttttttaacc tcttgtaacg tcttgatatt gctcttgacc cgtgttaatg
    21301 aggtgaaaaa tacatcattt ttgggaattg ccttaacttt gcacttgtag accggttcaa
    21361 aaaggtcacc attgtcatct agtgtaggag ccgtgtcttt gttatcaaag ctaatgctca
    21421 tatcatagtt tagagtcgta acgacctcta ttttttgttt ctcaatgatg atagcaatac
    21481 gttctgtcac attgatttta cttgccatgt tctttctcct gttaaaaaag tgtcgtttgc
    21541 aaagggtaca catcttcaaa cggtactcca agtcttagac agtcttgttt gatgtccatt
    21601 gtagaaatca catacttgac gccattgttt ttcttgtcgt aatgtggaaa agtgtacccg
    21661 tcattttcaa tttttatctt gatatccgtt ttggtttcag gtttccaatc cacccaatcc
    21721 gtccactcca tctcatacct catcaaataa acttaattga gcgttgtggt tttttatccg
    21781 ctcctctgct ataccaaaat aatattcatc tatttcagtt cctataaaat gcctatccgt
    21841 ttctaacgct gctaatgctg tcgtaccgct tcccatgaaa ccatcaaaaa cagtatcccc
    21901 aacatcagaa tgtttaacaa tacatcgttt tatcaattca ataggttttt gattttgatg
    21961 gagtagttca tctgaactta ctctcttgaa atcccaaaca tctgtcagtc gctcaccgtt
    22021 gaatttcttc cgtcctttgt ttgccaatat aatcatctcg tactgttttc caaactgcgc
    22081 ctctaaatcg ccagcggtat gattattttt tcgccatata ataatatttt ttactgaaaa
    22141 atacttttca atttcttttt taaaaaaatc aaccttgtca aatgaacaga atatataaat
    22201 agccgtatca tcttttaaaa ttcgataaca ctcttttata tactctttta tcaattcagg
    22261 attgttatca tttttgataa catttgaaaa tttatgcttt tctttccgcc aatttgtttt
    22321 ataatttatc aaataaggcg ggtctgttac gattaaatca attttattat caggaattgt
    22381 ttccataaat tccaagacat ctttttttat aatcttatct atttccacta actgtccctc
    22441 ctaaaacggc aaaccgtcat ttgggaggtc aaaggggtta ggatcggtaa aaggtgagct
    22501 attcccattt tggaaactgt tgccttgtcc gtgctgactg ttgcgactct ctagcagagc
    22561 tacactctca gcgattacca cagtcacata tcgacgctga ccgtctttct cgtaagatct
    22621 aacttgtatg cgtccaatga tcccaataag agagcccttg ctgcaatact gagcaatgat
    22681 gtcagctgta cctctccaag cttgaaaatt gataaaatca gcctcacgct ctccattttc
    22741 gtttttgaaa ttgcgattga ctgcaagcgt gccctgtaaa ctagatacat tattaggcat
    22801 ttttcgtaga tcaggaggcg ctacaagcct cccaaccagt gtgacgttat tgatcatctg
    22861 atttgtcccc ctctagtgct acgctctccc aagagatacc ctaaaaacat ccataggata
    22921 gccagcccaa tctctttgat aaaatcattc attatttttc tcctttgcat tcataacata
    22981 cattttgacc tacatctttt cccttgatta ttgataagct accacatttc tcacagctga
    23041 ttatgaaacc taaaccattt gaattaatac tgcttatatt gttctctgag ggaactttgt
    23101 aaataatcaa tgcggatgta tgccaatatt cagcactgac tccactgtca gcgacagcag
    23161 acacatttga ttgaaatttg atgtcaatca acttaatgcc tggattttcg gcaagccagc
    23221 tatttatttg gtcgtcaatc gcctcatgat gtggataatc acatgaaaaa aatacggttt
    23281 taatcatatt cccctcctgg attgtgccac cagatcatca ggtcttcctg attatctctg
    23341 atgtactgct caaatatttc aaagtggacg atagcatgtt ttaagcgttg cataccctct
    23401 ccagattttg agcaaaagct gaaaacttta aagacaggct caatcatgtc aataatttct
    23461 acgacttggc cattgaggtt ccagacgcta tcctctccca cattgaaatc aaggataaac
    23521 tcatccccta ggttgtggat aacctgcaat ttcttgccgt ccgagtagat ggctacgctg
    23581 tcagatattt ttctgatgtc catggttacc tccccattga ctctggagaa atatccaaga
    23641 tttttctatc tccaatttcc tttttttcgc catacagagt cgctaatagg tcctctattt
    23701 ttcctattaa ctcatcaggc accccatatt cagccaattc ttctgaaatt ttttcaattt
    23761 ctgtcatact taccacccac attgttcatt tagttcagcc tgagttaatg gctcgatacg
    23821 ttgataaccc ctgacttgat agttcttttt aaaatcaaat ccgagttgac ttagaccagc
    23881 cttgaaacgg tctttttcgg ctgtgtctac aaaatacacc tctaaagtca ttttttgggc
    23941 atatcgtttt aggtcatttt cagcccctct aagagcgtta ggctcatttt ggaggatttg
    24001 tccaccgtcc aagattttgc ccgtttctgg gtcaaaattt ggggtttccg ttgattttgg
    24061 agcctgttct tgctgtttgg tttgttgggc tgctaaaagt tcctgacttt ctcgctctgc
    24121 tcgttcttga gcctgtctga tttcttcctt ttgcttttca aactcataat cagctttgat
    24181 ttgtccaaag acttcagcaa gagtcaagtc tttcagctgt ctaatgtaag gtgagtcagt
    24241 catgccatac tcagcacata accctgaaat agctgacttg gctttttcaa attcttgttg
    24301 tttctgaaat tcaaatgtga ccatgtcatc aagtgacttc atagtggctt ttttaagcgt
    24361 cacgccatct gccataaaat cgctagcttt gacatactca agggcctttt catcaaagag
    24421 acgaggatcc agcatgtact cagctgattt gttggctagg tagcctttga ctgtgtcaat
    24481 tcggacagcc ttttgatgtt cttcaaactc tttgacatca ccagcaattt tggtaatgat
    24541 gtcttttaga ggctggatgg cattcttgac atacttgtca aattcatcag ctggttcaga
    24601 taagactttc ttattcctga tccgttcatc agaaacctgc ttgtctaatt ttcgtagatc
    24661 ggcaagtgtc tgcttgtcat ccttgatagt tgcagccgta accgtgtaat tttgatactt
    24721 tgctacaacc tcattgatat tctgctcaaa tttctcacgg tcaatgattt caacctgtgc
    24781 ttgtgttact tttacctgta attcttgcat gttgtcctcc taatattcaa gttcaccgtc
    24841 tagcaactcg ccctggattg gatcctcatt ttgagtaggt tcaggatctg catgatttgc
    24901 ctcttgctct ttgttgaatt gatcaatctg agccatcttg cgtgctacga catcctcacg
    24961 gctctcttga ggtgtgacgt ctttgattgc gtcaaatgta tctccaccat cgatgtctgt
    25021 atacatgttc ccaagttcgt cagggaaagc ctctctaaga gtctgcacta gagctgtttt
    25081 tctgatcata gtggctggca ttgatttcca agtgctccgt ggattgccat ttctgtctgt
    25141 tttgacatac tcattaaagt caactgtgac cttatatctg tgtgaacggt ctttacgata
    25201 gacaacggcc caaccaccta ttagaatgtc ctcaggtaat ttaagagagc cctcaatctc
    25261 tatcatttct ccatttctca tgacagtaat tccagcctca aaaccatcat atccctcaca
    25321 acgttcagca cgtttcataa atgcctcttt tgagacaatc aagctgaact ctgtgccgct
    25381 attgttttga taggctacaa tgtagacctc gttagcaaat gggttaagat tgcgaccttt
    25441 acacaaggct agagcctgac ctacttgttt ttcagtcagt aggttttgtg ggtcaaaata
    25501 ctttttgatg tctgccccag tcaataaact tggatcagta gtgatgtcac gttttatctg
    25561 tgttgctaat tgattattag tcatcttgtt ttctcctatg tgttcatgtc aattctgcgt
    25621 ctagacttac tatttaagtc atctaattca tttttgtaat cttgaataag ttgcaaattc
    25681 cggtcaatga agcgttctac aacttgacct agaagttctt gtgttgtcac gcctctcaat
    25741 tcagcaagaa gtctgatata ttctttttgt ttttcagaga tctctgctct tatgaacgac
    25801 tttcctttat tggtcatctg cgtcattttt ttctccttta ggctagcaat ctcctacata
    25861 gatccattga ccagcgctga aaatccaatc agctgggtca agttcttctc gttcttcagg
    25921 cggttgcatt atatctctgt cataattaaa catgagcata caccttccca agctccagaa
    25981 ctcgtttcac atatctagcc ttggatgtta gcccaagatc cagcaattcg tttttttctt
    26041 catgattggc caaaagccag acacggtttt caatttcaat ctttgtcatc ttcctgctcc
    26101 acctcttcaa ttttcacttc gctatttaga cgtttcatgg cttcatctac cgacttgccg
    26161 cccaggacgt ccttgagcat gtggcttaca tcgtgcattg tttgagcctt cgccttgctt
    26221 ctttcatcct ctggcatcaa tccgacatct tgtagagcca gaaaggctaa actgacatcg
    26281 tgcatttctt tctgaagctg tttgattttt ttgattgttt ttagtgcttt aaacatattg
    26341 ttctcccatc tgttcttttt ctttgtagat tgccaattgt tgtttcagat catagatttc
    26401 ttgctcacac ataaagcgac gtttgcgctc ttcgagaagg tcctcattaa gctctaccgc
    26461 tactactctc caatcaaggc tcacttcctt gatgattccc tcgagtctga gttttaactt
    26521 agtaagtgat ttcattaagc tacatcctcc ttgttagatc gcttgttcat acctagaatg
    26581 atgtcatagt acgaatgacc agcagggatg acatagcctg tcaagtcttc aatgaccgaa
    26641 ccatctgcca ttatgtttac aattcttggt ttccattgct ctttttttct cttcatgtta
    26701 taattccctt gaataatttt gttgagcgcc tgattgccgt caggtgcttt tttattttat
    26761 gtcgtataga cacttccatt cgtcgcataa tacgtcagct cattcatctt attagtgaac
    26821 cgttcgtctg tcgtaatcag caacctctct ttaagcaggg ttgatagtcc gtaaaattgg
    26881 ctttcaaact gttcaatagc ctgcttgcgt tcctcagtag tcagttgctg acaaggagcg
    26941 tctcgaagct gtgtctttgc tgaacttaaa agcattcgtc ttcatatttc ctttcgttat
    27001 tctgtcaacg agactctgct cgtaaagttc tttgaggtgc ttaccttcaa aattagttgt
    27061 gataattgta ttcgtcctgt tttcaagtat ttgatacagg actttctgca tccagttatt
    27121 gccttgtctg atttcgttcc caacactcga ctctttgcct aggtcgtcca aaatcaagaa
    27181 gtcaacgttt tgcaggaatt tcacgactga gcgttgttcc cacttagagt ccttgtattg
    27241 aaaagcctct tgcattcgag aaaacagctc catggatggc atatagacta ccgatttccg
    27301 aacttggagc atttgaaagc tctcgtttaa ggttttagct atcccgacgg ccagatggct
    27361 cttgccaact ccaggcggtc cagagataat cgtattccct tcgtagcgct ctttcacata
    27421 gtcagccgtg actcgcttag cgaaattgac tgctttagcg tcctgatttg tatggatttc
    27481 aaaatttcca acagtcgcat ttttcaaatc gtttgggatg atgctctctt tcataaagag
    27541 agaatatgat ctcgtatctc tgatttgagc ttcagcaata gctaactgtt cgcttgcgtt
    27601 ctggttgata gtctcttgaa cacattcagg acaataggtc agcgtgttgc gagtgcaagg
    27661 gttgatagac cgccacatgt atacaccttc gtgttttgga cattgctgtt tcaaagtctc
    27721 aacctgcaag gctctttctt gcaactcttt gcttgatact acttgcatgc gcacccccta
    27781 aaatccaagc cgtggatcaa atccatcatc ggataatctc aagcgaccgt tcgacttatt
    27841 gtttgaccga gtaggcttct gcctgttctc taccagttca acagtcgtta aacctttctg
    27901 tttccagtct ctcaatatac tttcaagata tttaaagtaa ggcttaccat tgcccacaca
    27961 ttccttgatg gctaacttga taacctcttt actatggtct tgtaagaagt atttcaagtc
    28021 ctcaatttca aatggtgtcg ggtatcgtcc gaattctgaa aaaatccaat catgaacaat
    28081 tcctaagtcg ttttctgcgg gggcgtcctc tatactatat agattattag cacccacccc
    28141 ctctggttca atcaggttgg ttatataagg gtggttatta tcaggttggt tagacttaat
    28201 atttttaagt tcttgaacta aaatttcttt agttcccccc ttaatatttt taagttcttg
    28261 aactaaaatt tctttagttc tgattttctg aggatatagc aaattcgcca atctaacgcc
    28321 ctgtcttttt tctttcaaaa gaccatgttc tatcagttgc ttttttaatt tgattacaac
    28381 tggctcgctt ttttttatcc acttaccaat ttcttcgtta gtagctacaa cgtacagata
    28441 tccatcttta tcagtaaaat gttgcttatt ttttcgagat aatgaaacgc gatcggtcaa
    28501 aataccataa accataacag ccagcggatc taaatctgaa aaataatcat cttctagcag
    28561 ccgatacggg attttaaagt atctttcgtg attatccatg tctatctctg taaaatattg
    28621 ttcattcata cctctcctaa actatgattt aattcgtatt ttttgcctaa aaaaataaaa
    28681 tccttttcga cattgtatag tcgagcaagt ttgtctaaaa gatccattgg aattttcgaa
    28741 ctatcatgct cgtacttcaa cagtgtttgt tgatgaatat taagtttgtc ggcaacttct
    28801 tttgcagata aattataatt tgttcttatt gctctcaatg tcattttcgg cacattccta
    28861 cctccttatt tttctatttg ttcctcgcaa ttctgctata ataaaagcag aaaggaggta
    28921 atgttatgac tgattatcaa ttagaagctt ctctgattgt ccttggcaaa gagtacgaaa
    28981 gagccaagaa agatggtaaa gaaagcttca gtatacatgt gtcgttcttt gatggactag
    29041 atactaattt ccatcttcaa gagtttgcaa gacaatatcc cgtaaggatt gtccgttcga
    29101 agcctttcca aataactttt ctaataaagt aatatcattt agagggaagg gattggtttc
    29161 aactctatcg ttaaacgtta aaacaacttc acaactctct agaaaacgat tcgtaaattc
    29221 cactcgctct actccgtcca aaaacattcc atcgacgaat acagcagggt gggtttttct
    29281 tgccgtcagc agcacatcgt gctctgatgt atttatcgca atcgtttttg tcataccttc
    29341 gtctcactct ctagcgccct gagttctatc tcatggctga cttgtttcaa tagcttctca
    29401 cacgctattt tagcttctct gtacgttgtg ttttcgctga tgaagtaatc agcaagttcg
    29461 atgattttat cttccatgac cgtctccaaa aatcggtctt aagaccgatg taaccccttc
    29521 aaaaacagta tatatttata ttatccttaa caagaaagga gctgatgcaa attggcaaaa
    29581 tttttgaagg ggactgtggt tcagtgattc agtttggcta ggtaaccaac gcgtttttac
    29641 tgcgagtgtg actgcacgga gcctgtcgct gactataaga gggactgcag ctctgcttat
    29701 agcgggactg acagataact accgagcgac actcaaagac tagccaaacc acgttgattg
    29761 cagtgctgga cgcatgacca gcgaagtttc aaccagttgc tttacaccga ctgtgaaacc
    29821 ttatcaaagt gtgcaagtct tgacctagtg taaagtaggt taagactttt tttattgctc
    29881 aggaacttgt gaatcgttca aagaaatctt agaatctagt tcgtccactt tctcagcaat
    29941 atatgtcacg gtcctcaata tctcattgag ggctgttctt tctaattcgt ccatctttct
    30001 tctcctttct ttttcttttg ctctatgagc aacaacctgc caaggattcg aaccttggtg
    30061 ataccaatca ggctacattc attttgtcct gcattcctac aaatgctgca tcgaaacgaa
    30121 tatcatcgat ttcatcttga gtaaaaccag aatcgagaag gtaacgctct tggcactcga
    30181 tctcttctgc taactctgtc catccgaaag cgaactgacg gcagttagta cagaaagctt
    30241 caagtttgct atagaggaaa atttcctcgt aagtgccttg gattaaagtt tccttagcta
    30301 ctgctttgaa gatgttgatt gctttctcgt ttaatgtgtt catggtgttt ccctccgatt
    30361 tgttttttgt tatttcctta agcttgatta tattatacta cgatttaaat cgtatgtcaa
    30421 taactttttc gaattttttc gtaatttttt cgaatttttt atttacaaaa tcgaaaataa
    30481 acggtattat atagtaaaga agataggagg aaaaaacatg gcaagaggac gaggaaaatt
    30541 aactcctcaa gataaagagg atatgaaagt cttttccgca aatcttaact caattttatc
    30601 tgatagaaat tgtaaacaag ctgagctatc tcgagcgaca ggaataccac ctagcacatt
    30661 gacagggtat gtaaaaggaa cttctttgcc aatccctggc aatgttcaaa aaattgcaga
    30721 tttttttgga gtacctaaat ctgtattaga ccctagattt gtaactaata attctatggt
    30781 cgatgactct tcttctaata cttcctctat ccaaaccatc tacgaccaac tagaaccgcc
    30841 tagacaaggc aaagtcctga cttttgccga gaggcaacta aaagagcaaa acaaagaaga
    30901 aacgaagata aacgaagtat cggagaacat catcagactg gacgactaca gacagactac
    30961 ttaccgacgt gttactgggg ttgtctctgc tggtagtggt tcgatacagg acgacgattt
    31021 agatatggag gtttcgttct atgaggatga aatcccagac gactacgacg ctatcgctta
    31081 tgtcgtcggc aactctatgg agccaaagat aaagaatggt gactatcttt ttataaagaa
    31141 tacccaacag gttgattata acactatcgg catcttccaa gtagacggcg ctaactatgt
    31201 taagaaactg cgtcagggat atctggaaag cttgaatcca gattatgagg atatacacct
    31261 agacgaaagc aacgacatcc gaactattgg ggaagttgtc agtgtgtata gggagaaata
    31321 acatgagtaa cgaaagtaga ccgatggaag tgattaaaca caacctagat tgcaaatgcc
    31381 acagacgaag agagtggatt agagtaaatg ataaatggca tgctatcgag ttttcggtag
    31441 acgatccaaa cgaacctcct atgacagagg aagagaaagc caacgtggcc ttaattcttc
    31501 aacaacactt atcgaaagaa taaaaccaac tgtttccaaa atggaaataa ttgcaaacaa
    31561 aaaaaaagcc ccacgctctc aaaactttgg cgagtctgag cgtgaggcat gtgacaggaa
    31621 aagattttca tggagataac ctctcatgat gtcttttctt gtacccattt tatcattttt
    31681 taggaaattt tgaaagaggt actactatga taacaacaaa taaagtagct atatatgtca
    31741 gggtatcgac gacaaaccag gttgaggagg gatactctat agatgagcaa aaagacaagc
    31801 tctctagcta ctgcgacatt aaagactgga atgtatacaa agtatatact gatggaggtt
    31861 tctcaggatc caatactgac agaccagcgc tagaaagtct tatcaaagac gctaaaaaaa
    31921 gaaaatttga cacagttcta gtctataagc tggaccgtct tagccgtagt caaaaagaca
    31981 cgcttcactt gattgaggat gtattcatca agaatgggat tgaatttctg agcttgcagg
    32041 agaactttga cacctctact ccttttggta aggctatgat tggactcttg agcgtctttg
    32101 ctcagctaga aagggagcaa atcaaggaac gcatgcaact tggcaagcta ggacgtgcca
    32161 aatctggtaa atccatgatg tgggctaaga catcctatgg ttacgattac cacaaagaga
    32221 caggcacagt gaccatcaat ccagctcagg ctctgaccat taagtttatc tttgagagtt
    32281 acctgagagg gagatctatt actaagttga gagatgatct aaatgagaaa tacccaaagc
    32341 atgtgccttg gagctatcgg gcggtcagaa ccatactcga taaccctgtc tattgtggtt
    32401 tcaatcagta taagggagaa atttatccag gtaatcatga gccgattatt tcaaaagagg
    32461 aatacgataa gactcaatct gagctaaaaa taagacaaag aacagcagca gagaatgtca
    32521 atcctagacc attccaagct aagtacattt tatccggtat cgcccaatgt ggatattgtg
    32581 gcgctccttt aaaaattatg ttaggcgtaa agaggaaaga cgggagcagg ttaaaaaaat
    32641 atgaatgcca ccaaaggcac ccacgaacgc tgagaggcgt tactacctac aacgacaata
    32701 aaaagtgtga ctcaggattt tactacaaag acaagctaga ggcctatgtg ctaaaagaaa
    32761 taagcaaact acaagatgac gctgattacc tggacaaaat attttcagga gacaatgctg
    32821 agaccataga ccgtgagagc tataagaaac aaatagagga gctatcaaag aaactgagca
    32881 gacttaacga tctatacata gatgaccgca ttacccttga agaattacag agcaagtcag
    32941 ccgaatttat aagcatgagg gggactcttg aaactgaact agaaaacgat ccagcactca
    33001 ggaagaacaa aagaaaggct gatatgagga aactgctaaa cgctgagaaa gtcttttcaa
    33061 tggactacga aaatcaaaag gtgcttgtta gaaggcttat aaacaaggtt aaggtgacag
    33121 ctgaggacat tgttatcaat tggaaaatat aaataatttt agtaacctac at
//
LOCUS       DAVLFB010000011        33096 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLFB010000011 DAVLFB010000000
VERSION     DAVLFB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.011"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            58..339
                     /locus_tag="VXD58_000464"
     CDS             58..339
                     /locus_tag="VXD58_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV0675212.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            468..1412
                     /gene="manA"
                     /locus_tag="VXD58_000465"
     CDS             468..1412
                     /gene="manA"
                     /locus_tag="VXD58_000465"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV0675213.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1434..2909)
                     /locus_tag="VXD58_000466"
     CDS             complement(1434..2909)
                     /locus_tag="VXD58_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV0675214.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3075..3239
                     /locus_tag="VXD58_000467"
     CDS             3075..3239
                     /locus_tag="VXD58_000467"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0675215.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3240..3746
                     /locus_tag="VXD58_000468"
     CDS             3240..3746
                     /locus_tag="VXD58_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV0675216.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3766..4221)
                     /locus_tag="VXD58_000469"
     CDS             complement(3766..4221)
                     /locus_tag="VXD58_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0675217.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4241..5416)
                     /locus_tag="VXD58_000470"
     CDS             complement(4241..5416)
                     /locus_tag="VXD58_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0675218.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFTGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5475..6320)
                     /locus_tag="VXD58_000471"
     CDS             complement(5475..6320)
                     /locus_tag="VXD58_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0675219.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6445..7002
                     /locus_tag="VXD58_000472"
     CDS             6445..7002
                     /locus_tag="VXD58_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0675220.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7021..7488
                     /locus_tag="VXD58_000473"
     CDS             7021..7488
                     /locus_tag="VXD58_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV0675221.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7577..8206
                     /gene="upp"
                     /locus_tag="VXD58_000474"
     CDS             7577..8206
                     /gene="upp"
                     /locus_tag="VXD58_000474"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV0675222.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8380..8970
                     /gene="clpP"
                     /locus_tag="VXD58_000475"
     CDS             8380..8970
                     /gene="clpP"
                     /locus_tag="VXD58_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV0675223.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9049..9297
                     /locus_tag="VXD58_000476"
     CDS             9049..9297
                     /locus_tag="VXD58_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV0675224.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9398..10558
                     /locus_tag="VXD58_000477"
     CDS             9398..10558
                     /locus_tag="VXD58_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0675225.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10826..11695
                     /locus_tag="VXD58_000478"
     CDS             10826..11695
                     /locus_tag="VXD58_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0675226.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11699..12655
                     /locus_tag="VXD58_000479"
     CDS             11699..12655
                     /locus_tag="VXD58_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV0675227.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12655..13419
                     /locus_tag="VXD58_000480"
     CDS             12655..13419
                     /locus_tag="VXD58_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675228.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13419..14129
                     /locus_tag="VXD58_000481"
     CDS             13419..14129
                     /locus_tag="VXD58_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675229.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14543..15199
                     /locus_tag="VXD58_000482"
     CDS             14543..15199
                     /locus_tag="VXD58_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0675230.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15307..16402
                     /gene="prfB"
                     /locus_tag="VXD58_000483"
     CDS             join(15307..15378,15380..16402)
                     /gene="prfB"
                     /locus_tag="VXD58_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV0675231.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16420..17112
                     /gene="ftsE"
                     /locus_tag="VXD58_000484"
     CDS             16420..17112
                     /gene="ftsE"
                     /locus_tag="VXD58_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV0675232.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17105..18031
                     /locus_tag="VXD58_000485"
     CDS             17105..18031
                     /locus_tag="VXD58_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675233.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18317..20497
                     /locus_tag="VXD58_000486"
     CDS             18317..20497
                     /locus_tag="VXD58_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0675234.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20549..21364
                     /locus_tag="VXD58_000487"
     CDS             20549..21364
                     /locus_tag="VXD58_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0675235.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21504..22847
                     /locus_tag="VXD58_000488"
     CDS             21504..22847
                     /locus_tag="VXD58_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0675236.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23063..24253
                     /locus_tag="VXD58_000489"
     CDS             23063..24253
                     /locus_tag="VXD58_000489"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV0675237.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSEEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIKK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24806..25741
                     /locus_tag="VXD58_000490"
     CDS             24806..25741
                     /locus_tag="VXD58_000490"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV0675238.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25775..26812
                     /locus_tag="VXD58_000491"
     CDS             25775..26812
                     /locus_tag="VXD58_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV0675239.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            26985..27590
                     /locus_tag="VXD58_000492"
     CDS             26985..27590
                     /locus_tag="VXD58_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV0675240.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELTAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27747..28277
                     /locus_tag="VXD58_000493"
     CDS             27747..28277
                     /locus_tag="VXD58_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV0675241.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRVSFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28298..29200
                     /locus_tag="VXD58_000494"
     CDS             28298..29200
                     /locus_tag="VXD58_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV0675242.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVSCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDID
                     AACGQLRFNTMKRDRQKAVAAVNP"
     gene            29200..29682
                     /locus_tag="VXD58_000495"
     CDS             29200..29682
                     /locus_tag="VXD58_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0675243.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQPL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            29684..31225
                     /locus_tag="VXD58_000496"
     CDS             29684..31225
                     /locus_tag="VXD58_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0675244.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31281..32084)
                     /locus_tag="VXD58_000497"
     CDS             complement(31281..32084)
                     /locus_tag="VXD58_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV0675245.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(32168..32381)
                     /locus_tag="VXD58_000498"
                     /pseudo
     CDS             complement(32168..32381)
                     /locus_tag="VXD58_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32860..33060)
                     /locus_tag="VXD58_000499"
     CDS             complement(32860..33060)
                     /locus_tag="VXD58_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675246.1"
                     /translation="MNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDLLVD
                     DSLAGDIILLWRIQFGTFTTET"
BASE COUNT        10062 a         6166 c         7209 g         9659 t
ORIGIN      
        1 ttatataatg ctttttgatt ttagtatagt attatcgtag aaaatggact cggtgctatg
       61 gatacaccgg atgagaatgg ttatgtagca gatgactatc ggattactta cttagaggcc
      121 cacatcaagg ccatgcgaga tgccatttac aaagacgggg ttgacttgct tggttatacg
      181 acatggggct gtatcgattc ggtttcagct ggcacagggg aaatgaataa gcgctatggt
      241 tttatctatg tagatcgaga taatgtagga aatgggactc tcaaacgtag caagaagaag
      301 tccttctatt ggtacatgtc atttatagca atggtgtaag catcgagtag agcacatttg
      361 tttactattt ttataaaatc ttctcccctc attatagcaa aaaaagagtt caattcgaac
      421 tctttttttg ctataatgag gggagaaaaa tcagacagga gattaagatg tcagaaccat
      481 tatttttaca atcagttatg caagaaaaaa tctggggtgg taccaagcta cgtgatgagt
      541 ttggctacga catcccaagt gaaaaagtcg gagagtactg ggctatctca gctcatctaa
      601 atggtgtttc taaagttgcc aatggtcgtt acgagggaac agatcttgct actttgtatg
      661 cggaacaccg tgaattattt ggcaatcgtc ctgagcctgt atttccgctt ttgactaaga
      721 ttttggatgc taacgactgg ctcagcgttc aagttcaccc agacgatgct tatggactag
      781 agcatgaagg cgaacttgga aaaacagaat gctggtacat catcgctgcg gatgaaggtt
      841 cagagattat ctacggccac aatgccaagt caaaagaaga actccgccag caaatcgagg
      901 ataagaactg ggatgcgctg ttgacaaaag tgccagttaa agcaggagat ttcttctatg
      961 taccaagcgg taccatgcac gctatcgggg cgggtatctt gattcttgaa acccagcagt
     1021 ctagcgatac cacttaccgt gtctatgact ttgaccgtaa ggatgacaag ggcaatttac
     1081 gtgaacttca ccttgaaaaa tctatcgatg tcttgaacat tggtgaaccc gcaaatagcc
     1141 gtccagtgac aatcaaagca gatgatctac gttccactct ccttgtatcc aatgatttct
     1201 tcgcagttta caaatgggaa attactggaa aagttaactt tgaaaagaca gctgactaca
     1261 gcttgttgag tgtcttggct ggtcaaggtc aactaactgt tgacgggaaa aactatccaa
     1321 tccaaaaagg gagacacttt atcctaccaa gtgatgttga agcttggact ctggaagggc
     1381 aagatttaga attaattgtt agtcatccat aaaaaagaaa gggcctgagt tcattactca
     1441 agtccttttt gattacataa attgggcgat gaagaccacg atgatgattg gaatgacgga
     1501 acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca tgctctttca
     1561 tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa
     1621 aggtagagag ctccaaatgg catgaggaga ttgggaacca agaagttcat agcgtcaaag
     1681 aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc tgaaggaatg
     1741 ccaaagacaa aggtcaaaat tcctaaaata acactccatt tggcacgttt gctgttatcc
     1801 tggttggtga tattgcccac attgatctcc agcatcacga cagaagaagt gacagtcgca
     1861 aagaggaaga ggacgtagaa aatggttcca aaaggcatct tgtcaaagag ttgaggcaag
     1921 acgataaaga gcaagctcgg tcccccttca gactggatat tgaaggggga ccgagcttgg
     1981 aaaatggcta gacctgccat gatggatatc gagatattca tggctacgat ggagattcct
     2041 gactggacta gattggtttt cttgtccaag taagaagcat aggtcaacat gactgtaacc
     2101 cctagtgaga gggcaaagaa agattgtccc agagcataga ggagaccagc gctagtcagt
     2161 tttgaaaagt ctggtttgag gaagtaaaga accccttcca tggcatttgg caaactgaga
     2221 gagcgaccga tgataacaac aaagatgata aagagcaggg gcatcatgac tttcgaagct
     2281 ctttcaatcc ctttttgaac cccacgtgat acaataaaga tatttaataa gataaaggct
     2341 gcttgagctc ctagggcaat ggctggattt gaaatgattg aagtaaataa ctgagcataa
     2401 tcacccgttc caccaagttg gaacaatttc ccaaactcaa tacctagata gactagaatc
     2461 catcctccga taacactgta aaaagataag aggataaaga gggcaaaggc gccaatccaa
     2521 ccgataaagt tgtacttgtt attcttgccc agttttccaa aggttttgat agcggaaacg
     2581 ccagcactac ggccaagggc aaactcagcc agcaggagag ggaaaccgat taaaatagtg
     2641 gaaatgagaa agattagtaa aaagcctcca ccgccattag cagcagtcat gtagggaaac
     2701 ttccaaacgg aaccaagccc gatgaccgag ccagcagatg ctagaataaa gccaagtttc
     2761 gacccccatt gtgatttttc tgacatagtt aaactctgat ctcctttttc aaaataagaa
     2821 aaagctactt gagttgtcaa atagccttct gtttgattga tagacttgac tatcctatta
     2881 tgttttctaa ggtctgtcaa gaaaacaatt ttcaagtttt cacacctttc tcaaaaacgc
     2941 ttgactctga cctaagggga ggggttatac tatcattgta aggaggaaat catgtaccat
     3001 ataaaagaag ctgcgcagct ttcaggtgtc tctgtcaaga ccctgccctg catcactatg
     3061 acaagatagg actcttggtc cccttaaagt cggaaaacgg ctatcgaacc tatagtcaag
     3121 aggatttgga acgccttcag gtcattcttt actacaaata tctaggcttt tctttagaga
     3181 aaatagcaga actgttaaag gaagaaagga cagatttatt gctccatttg actaggtagt
     3241 tggactatct aactcgcgaa aggcaacatc tggatacctt gatttccacc ttgcaaaaaa
     3301 ctattcaaga acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc acgggattta
     3361 gctatcaaga caatcaaaaa taccaccaag aagcggtaga gaaatatggt caagaagtca
     3421 tgggacaagc gctcgaacgc caaaaaggtc acgaagacga ggctacggcc gcctttaacc
     3481 aagtctttca aactttggca caaaatcttc aagttggttt acctgcaaca gcaaccgaaa
     3541 accaggagca agcagccaag ctcttgcaag ccattcgcac ttatggattt gactgctcta
     3601 ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa cccagagttt aaggaaaaca
     3661 ttgacaagtt tggttctgaa acagcccagt acacgtcaga tgccattgcg gcttacgttc
     3721 agacaaatgc agaaaaaata ggctaggaat ttcctagcct attttttact tcaaatcata
     3781 aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt ctagaaacac
     3841 acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat tttcaaagtc
     3901 ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt gttctgtctt
     3961 gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga taccacagag
     4021 ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga cagcatcgac
     4081 aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc agtcgggact
     4141 aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt taacaaattc
     4201 gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa tgcagacttc
     4261 ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg tgggcattga
     4321 tatccataac ttcgcctgta aagtggaggc caggtaccag cttactttca agggttttag
     4381 gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca agggacattt
     4441 ttccagttac aggaatttta agttctttaa tggactggac aagttgttct cgttcctttt
     4501 cagtcagttg tttgactttt tcaggatatc cttgtacaaa aaattcggcc aagcgttctg
     4561 gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct agaaatgtaa
     4621 ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc ccacctttga
     4681 caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta aagagtaaat
     4741 catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa atcccttgta
     4801 aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga tcggtgatgg
     4861 tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc gaaggataag
     4921 acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc gctgacttaa
     4981 ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta gcaacttgac
     5041 cacctagttc ggtgattttc ttttccaaag cttcgataat agtccgagac ttgtcactgg
     5101 ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct gtaaaaaagt
     5161 tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt ccgtttccag
     5221 gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa cgtcccccac
     5281 cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg gttttctgtc
     5341 cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg atgacaatag
     5401 tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat ggtcacctct
     5461 tgtcaagaat gcaattaatc aatttcatag cccatcagca aaccgccctc ttctgcatag
     5521 aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg
     5581 attcgctctg agagctgttg acaacatttt tcgttattgc gttgggccat gacaatacgg
     5641 ccaccagcat atccagcttt tactaactca tcataggcag cttgaactga tttctttgat
     5701 ccccttgctt tttgtagcaa ttcgagagtt ccagtttcac tagcttttcc gaccatacga
     5761 atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt cttcaccaag
     5821 ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc agtgatagct
     5881 tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt ttctacgagt
     5941 aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt gtcaggatgg
     6001 tcttctagat aaatattctt tgctagttga gcactattgt gactgccaga aagggtacct
     6061 gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata gtcatctggg
     6121 cttggacaag ccgattttga agcttctgca gttgcataca tggtttccat catttggtca
     6181 atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt taaggggaca
     6241 cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca accagagtca
     6301 gcaataattt tccaagtcat agaaattctc catctttgtc agttatacat tgacaaaggt
     6361 tctgtctttt tttacaatta tatcatgaaa acccttgaaa caaaagcatc atccgtctgt
     6421 tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa
     6481 aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat tgaaacagcc
     6541 ctcttgcaac tcttggaaaa aaaggagctg accaagatta gtatttctga attggtcaaa
     6601 cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga ggaaatttta
     6661 gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca ttacgattta
     6721 aagacagacc tttatttggt ttgggttcac cttttccggg aggccagaaa ggaagctaga
     6781 gtgattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa
     6841 tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg
     6901 agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa ggtaccagct
     6961 gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag gagaaaagag
     7021 atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagccct actaattgcg
     7081 aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt
     7141 atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga tcacgaatgt
     7201 ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa
     7261 ggtgctgagc gtggtgttcc caaaggcttt acagcctatg taacccattt tccttgtctc
     7321 aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg tggtttatat caaccagtac
     7381 cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt gacccattta
     7441 ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa attatcaaaa
     7501 ataaatggct tagaaagatt tttaaaccgt tttttggtat aataagaaga ataaattgaa
     7561 agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga ttcaacacaa
     7621 attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc tagtagatga
     7681 gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag atgtggaaat
     7741 cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat tggccatcgt
     7801 cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg ttccagctgc
     7861 taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt
     7921 ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt atagtagacc caatgttggc
     7981 aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg catcaaatat
     8041 caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc
     8101 agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg gttatatcgt
     8161 tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc gaaaagaaga
     8221 atgacttgga aaagtatttc cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg
     8281 aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct tatcttcagt
     8341 catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt agttattgaa
     8401 caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa agaccgcatc
     8461 attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc ccaattgctt
     8521 ttcttggatg cccaagatag tacaaaagat atttaccttt atgtcaatac accaggtggt
     8581 tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc agatgtccaa
     8641 accattgtta tgggaatggc tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa
     8701 ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat gggcggtaca
     8761 ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct caaaactcgt
     8821 aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa agtccatgca
     8881 gatgcagaac gtgataactg gatgagcgcc caggaaacac ttgaatatgg ctttattgat
     8941 gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc gactgggttt
     9001 gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat gtttgaaaaa
     9061 gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa aaaactgcag
     9121 gattatggag atattaccta tcattccaag aaacatcgtt acttacaact ctatgttcca
     9181 actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt
     9241 agggtttgtc atatccaaga gttggaaaca ccctttgtgg gcaatcttta tcgataggaa
     9301 aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa ttcggtatat
     9361 tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat ttgccctatc
     9421 gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga agtctggagc
     9481 agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt ttaactttga
     9541 agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc aattggctgt
     9601 tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag tcgataaaga
     9661 taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa cccaatctaa
     9721 agtatcagca gtcgtaggac ctgcaacatc tggtgcgact gcagctgcgg tagcgaacgc
     9781 tacaaaagca ggtgttccct tgatctcacc aagtgcgact caagatggat tgactaaagg
     9841 tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa ttatctcaaa
     9901 ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca atgccagtga
     9961 ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa tcgttgcaga
    10021 tgaaactttc gtagcaggtg acacagactt ccaagcagcc cttacaaaaa tgaaagggaa
    10081 agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga ttgtaaacca
    10141 agcgcgtggt atgggaattg acaaaccaat cgttggtggt gatggattca acggtgagga
    10201 gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct caggcttctc
    10261 aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc gtgctaagta
    10321 caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa
    10381 cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat aaccttgcta aaacaaaaga
    10441 ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag tcaaaactgc
    10501 ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa aaccataata
    10561 gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat atttaatact
    10621 cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga ttatatatgt gactgacttc
    10681 gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt
    10741 ttgctctttg attttcattg agtataagaa cctatcaaaa agtgagggaa acccctcgga
    10801 attataaata gaaagagtga atcttatgct ccaacaactc gtaaatggtt tgattctagg
    10861 tagtgtttac gcgctgttag ccctaggata taccatggtt tacggaatta tcaagctcat
    10921 caacttcgcc catggtgata tttatatgat gggagccttt atcggttatt tcttgatcaa
    10981 ttctttccaa atgaatttct ttgtagcgct tattgtagct atgctagcga cagctattct
    11041 tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc cactctactc gtattgctgt
    11101 tttgattacg gctattgggg tttctttcct attggagtat ggaatggtct atctggttgg
    11161 tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt cgatatgatt tgggaccaat
    11221 tagcttaaca aatgtgcagt taatgatttt ggctatttcc ttgattttga tgattttgtt
    11281 acaagtcatt gtccaaaaga ctaagatggg gaaagccatg cgtgcagtat cagtagatag
    11341 cgacgcggcg caattgatgg ggatcaatgt aaaccgtacg attagcttta ccttcgcttt
    11401 gggttctgct cttgcgggtg cggctggtgt tctgattgct ctttattata actctcttga
    11461 gcctttgatg ggggttactc caggtcttaa atctttcgtt gccgcagtac ttggtggtat
    11521 cggaattatt cctggtgcgg ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc
    11581 gactgccttt gggatgtcag atttccgtga tgccattgtt tatggaatct tgttgttgat
    11641 cttgattgtc cgcccagctg gtatccttgg taagaatgtg aaagagaagg tgtaaacgat
    11701 gaaggaaaat ttaaaagtta atattctatg gttactcctt ttgttagctg gctatagctt
    11761 gattagtgta ctggtttcag tcggagtact taatctattc tatgtacaga ttttacagca
    11821 aattggaatt aatattattt tggctgttgg tctcaactta atcgttggtt tttcaggaca
    11881 attttcactt ggtcatgctg gtttcatggc gattggtgcc tatgcagcag ctattattgg
    11941 ttctaaatta ccaacctacg gtgccttctt tggagctatg cttgtagggg ctttgctttc
    12001 aggagcagtt gccttacttg tcggcattcc aaccttgcgc ttgaaggggg actatcttgc
    12061 ggtagcaact ctgggtgttt ctgaaattat ccgtatcttt atcatcaatg gtggaagcct
    12121 tacaaatggt gcggcaggta tcttagggat tcctaacttt acaacttggc aaatggttta
    12181 cttctttgtc gtgattacaa ccattgcaac cttgaacttc ttgcgtagcc caattggtcg
    12241 ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag tcagttgggg ttaatacgac
    12301 taaaattaaa atcatcgctt ttgtctttgg tgccattact gcaagtattg ctgggtcact
    12361 tcaggcagga tttatcgggt ctgttgtacc gaaagattac accttcatca actcaatcaa
    12421 cgttttgatt attgttgtat ttggtggact cggttccatt acaggtgcga ttgtttcggc
    12481 tattgttctg ggaattttga atatgcttct ccaagatgtt gctagtgtgc gtatgattat
    12541 ttacgctttg gccttggtat tggtaatgat tttcagacca ggtggactcc ttggaacatg
    12601 ggaactgagc ctatcacgtt tctttaaaaa atctaagaag gaggaacaaa actaatggca
    12661 ttacttgaag taaaacagtt aaccaaacat tttggtggtc taacagctgt tggagatgtg
    12721 actcttgaat tgaacgaagg ggaactggtt ggattaatcg gtccaaacgg agctgggaaa
    12781 accacccttt tcaacctttt gaccggtgtt tatgaaccaa gcgagggaac agtaacccta
    12841 gatggtcacc ttttgaatgg gaaatcacct tataagattg cctctttggg acttggacgt
    12901 actttccaaa atatccgtct ctttaaagat ttaacagttt tagacaatgt tttgattgct
    12961 tttggaaacc atcacaaaca acatgttttt actagtttct tacgcttacc agctttttac
    13021 aagagtgaaa aagaattaaa ggctaaagct ttggaattgt tgaaaatctt tgatttagat
    13081 ggtgatgcag agactcttgc taaaaatctt tcctacggac aacaacgtcg tttggaaatt
    13141 gttcgtgccc ttgctacgga acctaaaatt ctcttcttag atgaaccagc agcaggtatg
    13201 aacccacagg aaacagccga attgactgag ttaattcgtc gtatcaaaga tgaatttaag
    13261 attacaatca tgttgattga acacgatatg aatctggtca tggaagtaac agaacgtatc
    13321 tacgtacttg aatatggccg tttaattgct caaggaactc cagacgaaat taagaccaat
    13381 aaacgcgtta tcgaagctta tctaggaggt gaagcctaat gtctatgtta aaagttgaaa
    13441 atctttctgt gcattacggt atgatccaag cagtccgtga tgtaagcttt gaagttaatg
    13501 aaggagaagt tgtttccctt atcggtgcca acggtgcagg taagacaact attcttcgca
    13561 ccttgtcagg tttggttcga ccaagttcag gaaagattga atttttaggt caagaaatcc
    13621 aaaaaatgcc agctcagaaa attgtggcag gtggtctttc acaagttcca gaaggacgcc
    13681 acgtctttcc tggcttgact gttatggaaa atcttgaaat gggagctttc ttaaagaaaa
    13741 atcgtgaaga aaatcaagct aacttgaaga aggttttctc acgctttcct cgtcttgaag
    13801 aacgtaagaa ccaagatgca gctactcttt caggagggga acaacaaatg cttgccatgg
    13861 gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga tgaaccatca atgggacttg
    13921 ccccaatctt catccaagag atttttgata tcattcaaga tattcagaag caaggaacaa
    13981 ccgtcctctt gattgaacaa aatgccaata aagcacttgc aatctctgac cgaggatatg
    14041 tactggaaac agggaaaatc gtcctatcag gaacaggaaa agaactcgcc tcatcagaag
    14101 aagtcagaaa agcatatcta ggtggctaaa acaatccagt ggattgtttt agtcggcaga
    14161 tgaagattac gaagtaatca tcattatagt ctgggagact agtttaggtt gcagataaag
    14221 attgcgaagc aatcatcaaa tccagtggat tgttttagtc ggcagatgga gattacgaag
    14281 taatcatcaa tatagtccgg gggacctttt tagtcggtag attgagattg caaacaaatc
    14341 tgcatctaca ttgaaagctt aatttctaat aattgaaaaa atcgaatgaa aaatttctta
    14401 ccttcattca cagagctcga tttcagagct ctttttgcta gcttattcat acttttctga
    14461 atttcgaaaa agaaatgtaa gcgtttgata gatttacaaa aagattgtat aatagggata
    14521 agaatagaaa aggagaagtc tcatggcagt taaagatttt atgacccgca aggtagttta
    14581 tattagtcca gatacaacag tatctcatgc agcagatttg atgagagagc aaggtttgca
    14641 ccgtctgcct gttatcgaaa atgatcaatt agttggtttg gtgactgagg gaaccattgc
    14701 acaagcaagt ccatctaaag caacaagtct ttctatctat gagatgaatt atcttctgaa
    14761 taagacaaaa gtaaaagatg tcatgattcg cgatgttgtc actgtctcag gctatgctag
    14821 tctagaagat gcaacttatc tgatgttgaa aaataagatt agtattctcc ctgtcgtaga
    14881 taaccatcaa gtatacggag ttattactga ccgtgacgtt ttccaagcct ttcttgaaat
    14941 tgcaggttat ggcgaagaag ggattcgtgt acgctttgtt acagaagatg aagttggtgt
    15001 tcttggaaaa attgtttctt tgattgtaga agaaaatttg aatatctccc atacagtcaa
    15061 tattccgcgt aaggatggta aggtgattat cgaagtgcaa atcgatggat caattgattt
    15121 accagccttg aaagaaaaat ttgaagcaaa tggtattcaa gtggaagaaa tcgctcgcac
    15181 ttcagcaaaa gtcttgtaag aagggaagcc caaaggcttc ttttttcatg aaaaggggat
    15241 tagagaaaaa gatggaaaga aatgataaaa tatgctataa tgaaataatg taaaaaagga
    15301 gtatttatgg acatttcagt aattcgtcag aaaattgacg caaatcgtga aaaattagct
    15361 tctttcaggg ggtctctttg acctcgaagg gctagaggag gagattgcca tcttggaaaa
    15421 caagatgaca gaacctgatt tttggaacga taatattgca gcccaaaaaa cgtcgcaaga
    15481 attaaatgaa ttaaaaaaca cttacaatac cttccataag atggaagagt tgcaggatga
    15541 agtcgaaatt ttattggatt ttttggctga agacgagtca gtgcatgatg aactggtagc
    15601 gcagttagcc gaacttgata agataatgac cagctacgag atgactctac tcttgtcaga
    15661 accttatgac cacaacaatg ccatcttgga aatccatcca ggttctggtg gtactgaggc
    15721 gcaggactgg ggtgatatgt tgcttcgtat gtatactcgt tatggtaatg ctaaaggctt
    15781 taaagtggaa gtgttggatt accaagcagg tgatgaggct ggtattaagt cggtaacttt
    15841 atcatttgaa gggcctaatg cctatggtct cctcaagtca gaaatgggtg ttcaccgctt
    15901 agtgcgaatc tcaccatttg actctgccaa acgtcgccat acctctttca catctgtaga
    15961 agtgatgcca gaattggatg atactattga agtggaaatc cgtgaagatg atatcaagat
    16021 ggataccttc cgttcaggtg gtgccggtgg acaaaacgtc aataaggttt caacaggtgt
    16081 acgtttaacc cacattccaa ctggaattgt tgtccaatca acagtggatc gtacccagta
    16141 tggaaataga gatcgtgcca tgaagatgtt gcaggctaag ctctatcaaa tggagcaaga
    16201 gaagaaggct gcagaggtag attctctcaa aggtgagaaa aaggagatca cttggggaag
    16261 ccaaatccgt tcttatgtct tcacgcctta tactatggta aaagatcacc gaactagctt
    16321 tgaggttgct caggtagata aggttatgga tggggactta gatggtttta tcgatgctta
    16381 tctcaagtgg cgaattagct aagatagaaa ggaactcaca tgtcaattat tgaaatgaga
    16441 gatgtcgtta aaaaatacga caacggaacg actgctctac gcggtgtttc ggttagcgtt
    16501 caaccggggg aatttgctta catcgtagga ccttcaggag cagggaagtc aacttttatt
    16561 cgttctctgt atcgtgaagt aaaaatcgat aaaggaagcc tatcagttgc tggttttaat
    16621 ctggttaaga tcaaaaagaa agatatcccg cttctacgtc gtagtgttgg ggttgtcttc
    16681 caggattata aattgttacc aaagaaaact gtctatgaaa atattgctta cgctatggaa
    16741 gtaatcgggg aaaatcgccg taatatcaaa agacgagtga tggaagtttt ggacttggtt
    16801 ggattgaagc ataaggttcg ttctttccca aatgaactct caggtgggga gcaacagcgg
    16861 attgcgattg cgcgtgcaat tgtaaataat cccaaagtat tgatagctga tgagccaaca
    16921 ggaaatctgg atccggataa ttcatgggaa attatgaatc ttttggaacg gattaaccta
    16981 caaggaacaa ctattttgat ggcgactcat aatagccaga ttgtaaatac cttgcgccac
    17041 cgtgtcattg ccattgaaaa tggccgtgtc gttcgtgacg aatcaaaagg agagtatgga
    17101 tacgatgatt agtagatttt ttcgccattt atttgaagcc ttaaaaagtt tgaaacgaaa
    17161 tggttggatg acagtagctg ctgtcagttc agtcatgatt actttgacct tggtggcaat
    17221 atttgcatct gttattttca atacagcgaa actagctaca gatattgaaa ataatgtccg
    17281 tgtagtagtt tatatccgaa aggatgtgga agataatagt cagacaattg aaaaagaagg
    17341 tcaaactgtt acaaataatg actaccacaa ggtatatgat tctttgaaga acatgtctac
    17401 ggttaaaagt gttacctttt caagtaaaga agaacaatat gaaaaattaa ccgagataat
    17461 gggagataac tggaaaatct ttgaaggaga tgccaatcct ctctatgatg cctatattgt
    17521 agaggcaaac actccaaatg atgtaaaaac tatagccgaa gatgctaaaa aaattgaagg
    17581 tgtctctgag gttcaagatg gcggtgccaa tacagaaaga ctcttcaagt tagcttcatt
    17641 tatccgtgtt tggggactag ggattgctgc tttgttaatt tttatcgcag ttttcttgat
    17701 ttcaaatacc attcgtatta ccattatttc ccgcagtcgc gaaattcaaa tcatgcgctt
    17761 ggtcggagct aaaaacagtt atatccgtgg accgttcttg ttagaaggag cctttatcgg
    17821 tttattggga gctatcgcac catctgtttt ggtctttatt gtttatcaaa ttgtttacca
    17881 atctgtcaac aaatcgttgg tagggcaaaa tctatccatg attagtccag atttatttag
    17941 tccgttgatg attgccctac tatttgtgat tggggttttc attggttcat tgggatcagg
    18001 aatatccatg cgccgattct tgaaggttta ggtaaaatag ctgcttttat gaggagattg
    18061 taaaatctcc ttttttgcta caagagtttt tgaaaagaga tgcgcagaag aaaagagctt
    18121 ccaaagaagt cccccagaga agacttcttg atggctaatt tgaacttgaa atttggcgca
    18181 ctagaaattt tttagaaaaa agtgtaattt ttttataaaa gcctttacaa aaaaaattaa
    18241 agtggtataa ttaaattata gaaagtgcaa acgttttcgt aaaacgtttg cctaaataaa
    18301 aataaaggag atttgaatga tgaaagatac attcaaaaat gtcttgtctt tcgaattttg
    18361 gcaaaaattc ggtaaggctt tgatggtagt tatcgcggtt atgccggctg ctggtttgat
    18421 gatttcaatc ggtaagtcta tcgtgatgat taacccaacc tttgcaccac ttgtcatcac
    18481 aggtggaatt cttgagcaaa tcggttgggg ggttatcggt aaccttcaca ttttgtttgc
    18541 cctagccatt ggaggaagct gggctaaaga acgtgctggt ggtgctttcg ccgctggtct
    18601 tgccttcatc ttgattaacc gtatcactgg tacaatcttt ggtgtatcag gcgatatgtt
    18661 gaaaaatcca gatgctatgg taactacttt ctttggtggt tcaatcaaag ttgctgatta
    18721 ctttatcagt gttcttgaag ctccagcctt gaacatgggg gtattcgtag ggattatctc
    18781 aggttttgta ggggcaactg cttacaacaa atactacaac ttccgtaaac ttcctgatgc
    18841 actttcattc ttcaacggga aacgtttcgt accatttgta gttattcttc gttcagcaat
    18901 cgctgcaatt ctacttgctg ctttctggcc agtagttcaa acaggtatca ataacttcgg
    18961 tatctggatt gccaactcac aagaaactgc tccaattctt gcaccattct tgtatggtac
    19021 tttggaacgt ttgctcttgc catttggtct tcaccacatg ttgactatcc caatgaacta
    19081 cacagctctt ggtggtactt atgacatttt aactggtgca gctaaaggta ctcaagtatt
    19141 cggtcaagac ccactatggc ttgcatgggt aacagacctt gtaaacctta aaggtactga
    19201 tgctagtcaa tatcaacact tgttagatac agtacatcca gctcgtttca aagttggaca
    19261 aatgatcggt tcattcggta tcttgatggg tgtgattgtt gctatctacc gtaatgttga
    19321 tgctgacaag aaacataaat acaaaggtat gatgattgca acagctcttg caacattctt
    19381 gacaggggtt actgaaccaa tcgaatacat gttcatgttc atcgcaacac ctatgtatct
    19441 tgtttactca cttgttcaag gtgctgcctt cgctatggct gacgtcgtaa acctacgtat
    19501 gcactcattc ggttcaatcg agttcttgac tcgtacacct attgcaatca gtgctggtat
    19561 tggtatggat atcgttaact tcgtttgggt aactgttctc tttgctgtaa tcatgtactt
    19621 tatcgcaaac ttcatgattc aaaaattcaa ctacgcaact ccagggcgca acggaaacta
    19681 cgaaactgct gaaggttcag aagaaaccag cagcgaagtg aaagttgcag caggctctca
    19741 agctgtaaac attatcaacc ttcttggtgg acgtgtaaac atcgttgatg ttgatgcatg
    19801 tatgactcgt cttcgtgtaa ctgttaaaga tgcagataaa gtaggaaatg cagagcaatg
    19861 gaaagcagaa ggagctatgg gtcttgtgat gaaaggacaa ggggttcaag ctatctacgg
    19921 tccaaaagct gacattttga aatctgatat ccaagatatc cttgattcag gtgaaatcat
    19981 tcctgaaact cttccaagcc aaatgactga agcacaacaa aacactgttc acttcaaaga
    20041 tcttactgag gaagtttact cagtagcaga cggtcaagtt gttgctttgg aacaagtaaa
    20101 ggatccagta tttgctcaaa aaatgatggg tgatggattt gcagtagaac ctgcaaatgg
    20161 aaacattgta tctccagttt caggtactgt gtcaagcatc ttcccaacaa aacatgcttt
    20221 tggtattgtg acggaagcag gtcttgaagt attggttcac attggtttgg acacagtaag
    20281 tcttgaaggt aaaccattta cagttcatgt tgctgaagga caaaaagttg cagcaggaga
    20341 tctccttgtc acagctgact tggatgctat ccgtgcagca ggacgtgaaa cttcaacagt
    20401 agttgtcttc acaaatggtg atgcaattaa atcagttaaa ttagaaaaaa caggttctct
    20461 tgcagctaaa acagcagttg ctaaagtaga attgtaatat acttgaggtt ggaagctgta
    20521 ttccaacctc ttattttggg agaaaagaat gaaattttta acactcaata ctcacagttg
    20581 gatggagaaa gaagcagagg aaaaattcca gattttgctt gaagatattc ttgaaaagga
    20641 ctatgatttg atttgtttcc aagaaatcaa tcaggagatg acctcgtcag aggtggaggt
    20701 taatgacctt tatcaagctt tgccagcagc tgagcctatt caccaagacc attatgttag
    20761 actcttggtt gaaaagttgt tagagcaagg gaaaaattac tactggacct gggcttataa
    20821 ccatatcggc tatgaccgct accacgaagg tgtggctatc ttgtctaaaa cacctattga
    20881 agccagagaa attttggttt cagatgtgga tgatccaaca gactatcata ctcgccgtgt
    20941 tgccctagct gaaactgtag tcgatggcaa ggagctagca gttgccagtg ttcatctctc
    21001 ttggtgggat aaaggtttcc aagaagaatg ggcacgattt gaggctgtct tgaaaaaatt
    21061 gaacaagcca cttttactag ctggagattt taacaaccca gcaggtcagg aaggttacca
    21121 agctatttta gctagtccat taggcttaca agacgcattt gaagttgctc aagagaaaag
    21181 tggtagctat actgttccgc ctgaaattga tggctggaaa gggaacactg aaccccttcg
    21241 aatcgattat gtctttacta ccaaagagtt agcggtggaa aatttacatg tcgtatttga
    21301 tggtaacaag agtccacaag tgagtgatca ctatggcttg aatgctatat taaactggaa
    21361 ataataactg aaaagaggtt ggaactataa aattccagcc ttttcttact agagaagcta
    21421 ctggaaatag cctaaataag tgagactact gtaatggaat aaaatatggt ataattgata
    21481 aggtagatag aatcgaggat gttatgtcat ttacgaaatt tcaatttaaa aactatatta
    21541 gagaagcctt gaaggagtta aaatttacaa ctccaacaga ggtgcaagac aagttgattc
    21601 ctattgtttt ggcaggtcgt gacctagtag gagaatcaaa aacaggttca ggtaagactc
    21661 atactttctt gttaccgatt ttccagcaat tagatgaagc tagcgatagt gtacaagcag
    21721 tgattactgc accgagtcgt gagttggcta ctcaaattta ccaagtagcg cgtcagattt
    21781 cagctcactc agatgtcgaa gttcgtgtgg ttaattatgt gggtggtacg gataaggctc
    21841 gccagattga gaaattggca agcaatcagc ctcatattgt tattggaaca ccaggccgta
    21901 tctacgactt ggttaaatct ggtgatttag ctattcataa agccaagaca tttgttgttg
    21961 atgaagcaga tatgaccttg gatatgggat tcttggaaac tgttgataag attgctggca
    22021 gtcttccaaa agacttgcaa ttcatggtct tctcagcgac tatcccacaa aaactgcaac
    22081 cattcttgaa aaaatactta tcaaatcctg ttatggagaa aattaagacc aaaacggtta
    22141 tttctgacac cattgataat tggttgattt cgaccaaggg acatgataag aatgctcaaa
    22201 tttaccagtt gactcagttg atgcagccgt atttggcaat gatttttgtt aacactaaaa
    22261 cacgtgctga tgaattgcat tcatatctga ctgctcaagg cttgaaggtt gcaaaaatcc
    22321 atggagatat tgcccctcgt gaacgcaagc gaatcatgaa tcaggtgcaa aatctggatt
    22381 ttgagtatat tgtcgcaaca gatttggcag cgcgtgggat tgacattgaa ggtgtcagcc
    22441 atgtcatcaa tgatgccatt ccgcaagact tatctttttt tgttcatcgt gttggtcgta
    22501 ctggacgaaa tggcctacca ggtacagcta ttacccttta tcagccaagt gatgactcgg
    22561 atatccgtga gttggagaaa ttgggaatca agtttagtcc taagatggtc aaagacgggg
    22621 aatttcaaga tacctatgac cgtgatcgtc gtgccaaccg tgagaaaaaa caagataaac
    22681 ttgatatcga aatgattggt ttggttaaaa agaaaaagaa aaaagtcaaa ccgggttata
    22741 agaagaaaat tcaatgggcg gttgatgaaa agcgccgtaa aaccaagcgt gctgaaaatc
    22801 gcgctcgcgg tcgtgcagag cgtaaagcta aacgccaaac attttaatag aaattgttgg
    22861 agtattgagc tccaactttt ttatttatga gaacgaacta tctaaaccga aacactacat
    22921 taaagactgc aaattgcgat taaaaatggt ataatgataa agttatatag tcccgataag
    22981 atggtaggta tttattacga agagttttcc tatcagtact ttgtaactct ataacaatat
    23041 tttttaaggg ggggacattt ttatgtcaga gcgtaaatta ttcacgtctg aatctgtatc
    23101 tgaggggcat ccggataaga ttgcagacca aatttcagat gcgattttgg atgctatttt
    23161 agcaaaggat ccagaggcgc acgttgctgc tgaaacagct gtatatactg gttctgtcca
    23221 cgtttttggt gaaatttcta caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac
    23281 cattgcagag attggttata ccaatacaga atatggattt tctgctgaga cggtgggagt
    23341 acacccatct ttggtggaac aatctcctga catcgctcaa ggtgttaacg aagccttgga
    23401 ggttcgtgga aatgctgatc aagatccact ggacttgatt ggagcaggtg accaagggct
    23461 catgtttgga tttgcagtag atgaaacaga agagctcatg ccattgccaa ttgcactcag
    23521 tcataaattg gttcgtcgtc tggcagaact tcgtaagtct gaagaaatta gctatctccg
    23581 tccagatgca aaatcacaag ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt
    23641 agatacagtc gttatttcta ctcagcatga tccagaggcc actaatgaac aaatccatca
    23701 agatgtgatt aaaaaggtca tcaaagaagt tattccatct tcttatcttg atgataagac
    23761 aaaattcttt atcaatccga caggtcgttt tgtaatcggt ggtcctcaag gggactcagg
    23821 tttgactggt cgtaagatta ttgtagatac ttatggtggc tactctcgtc atggtggtgg
    23881 tgccttctct ggtaaagatg cgactaaggt ggatcgttca gcctcttatg cggctcgcta
    23941 tattgccaag aatatcgttg cagcagacct tgctaagaag gcagaagtgc agttggccta
    24001 tgctatcggt gttgcgcaac ctgtttctgt tcgtatcgat actttcggta caggaacagt
    24061 agctgaaagt caacttgaaa aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat
    24121 tatccaaatg ctggacctca agcgtccaat ttaccgtcaa acatcggctt acggtcacat
    24181 gggacgtaca gatattgatc ttccatggga acgtttggat aaggtagatg ctttgaaaga
    24241 agcagtaaaa taagatttta agaggggaac gtcctctctt ttttatagtt tttaactata
    24301 ctgggatact gttctgaaaa tccattttgt gaaagtagag atttacatgt atagtagatt
    24361 gaaactagaa tagtacacct caacttctaa aacattgtta gaaatcgatt tgactgtcct
    24421 gattgatttc tcctgttctt gtttcatttt actatatttc tttaaaaatg ataaaggttg
    24481 agatttctcc tcgtaataga taatcatggg gatatttcaa tccaaagttt tattcgttat
    24541 cacttgacta ttgcaaggtt ttctagagca acagagtcat ggaatggact catggttgag
    24601 atttctcctt gttgcttgga cttcattcaa aagtctgtta cccaagcctt gttcaaactt
    24661 ccaatacact agctgtttcc atagcatgac ttctgtacta gactttcttt tccgaataaa
    24721 tagatagaac cacagaatct agtaaaccta gaattaaaat tatggtataa tattagcaat
    24781 aaaagaaatc tggaggatta gaatcatggt atcaacgaaa acacaaattg ctggttttga
    24841 gtttgacaat tgcttgatga atgcagcagg tgtggcttgt atgacgatag aggagttaga
    24901 agaggtcaaa aactcagcgg caggaacctt tgttactaag acagcgacct tggacttccg
    24961 tcaggggaat cctgagccac gctaccaaga tgttccactt ggttccatca actctatggg
    25021 cttgccaaat aatggcttag actattattt ggattatctt ttagatttgc aggaaaaaga
    25081 gtcgaaccga actttcttct tatctctggt cggcatgtct ccagaggaaa cccatactat
    25141 tttgaaaaaa gtccaagaga gtgattttcg tggtctgact gagctaaatc tttcctgtcc
    25201 aaatgttcca ggtaaacctc agattgccta tgattttgag acaacagacc ggattttggc
    25261 agaagtgttt gcttacttca ccaaacctct tggaattaaa ttgccacctt attttgatat
    25321 tgttcacttt gatcaagcgg cagctatttt caacaaatat ccgctcaagt ttgtcaactg
    25381 cgttaactct atcggaaacg gcctctatat agaagacgaa tctgtcgtta ttcggcctaa
    25441 gaatggtttt ggtggaattg gtggagaata catcaaaccg actgctctag ccaatgttca
    25501 cgccttttat caacgtttaa atcctcaaat ccaaattatc ggaacaggtg gcgttctgac
    25561 tggtcgagat gcctttgaac acatcctctg tggagcaagt atggtgcagg tgggaacgac
    25621 ccttcacaaa gaaggtgtca gtgcttttga ccgcattacc aatgaactga aagcaatcat
    25681 ggtggaaaaa ggctacgaga gcttagaaga tttccgtggg aaattgcgct atattgacta
    25741 aattaaatcg aaaaatctga agaaaggaga gacgatgcta gccattgaag aaagtcagaa
    25801 gttgacttta tcaaatttac cgagcctgag cctatttaca gggacagatc agggtcagtt
    25861 tgaagtgatg aagagtcaag tgttgaaaca gattgggtat gattctgctg acctcaactt
    25921 tgcctacttt gatatgaaag aagtagttta caaggatgtg gaactggagt tggtcagcct
    25981 tcctttcttt gcggatgaga aaatcgtgat attagattat tttatggata tcacgactgc
    26041 taagaaacgc tttttgacag atgatgagct taagtcattt gaggaatacc ttgacaatcc
    26101 ttctccaaca accaagttga taatctttgc agaaggaaag ctggatagca aaagacggtt
    26161 agtcaaatta cttaagcgtg atgccaaggt cttcgatgca gtagaagtaa aagaacaaga
    26221 attgcgccag tatttccaaa agtggagtca gaaacaaggt ctgcagttta ccaatcattc
    26281 ttttgaaaat ctcctcatca agtcggggtt tcaatttagc gaaatccaga aaaatcttct
    26341 ctttttacag tcctataagg cgaattctgt tatcgaggaa gaggatattg ttaacgcaat
    26401 tcccaagacc ttgcaggaca atatttttga tttaactcag tttattctga ctaaaaagat
    26461 ggatcaggcg cgcgatttgg tgagagactt gaccttgcaa ggggaagatg aaatcaaact
    26521 gattgcagtc atgctgggac aatttcggac ttttactcag gtgaagattt tggcggagtc
    26581 tggccaaaca gaatcgcaga ttgcaagtag tttaggtagt tatctgggac gtaacccaaa
    26641 tccttatcaa atcaagtttg cattaagaga ttcgagagga ctttctttga gctttctgaa
    26701 gcaagctatt tcctatttga ttgagacaga ctatcagatt aagacaggtc tttatgaaaa
    26761 aggtttcctt tttgaaaagg cactcttaca gattgctagt caggtcaatt gacatttgtt
    26821 gaaactacaa cccacgtcag gattatcttg tcgtgggttt cttgctgatt tgcttatttt
    26881 attagaaagt atgtcagtga tttccttatc tataagcaaa aaacttgaaa aaagtgaatg
    26941 tttgcgttat cattttcata tagaaagaaa aagaggtatt acagatggct attatcttac
    27001 cagaacttcc atatgcatac gacgctttgg aaccatacat cgatgcggaa acaatgcatt
    27061 tgcaccatga caaacaccat caaacttatg tcaacaatgc caatgcagct ttagaaaaac
    27121 accctgaaat cggtgaagac cttgaagcct tgcttgctga tgtagaatct atcccagctg
    27181 atatccgtca agcacttatc aacaatggtg gcggacactt gaaccacgct cttttctggg
    27241 aattgatgac tcccgagaaa acagctcctt cagcagaact gacagcagca atcgatgcaa
    27301 catttggttc atttgaagaa ttccaagcag ccttcactgc agcagcaaca actcgttttg
    27361 gttcaggttg ggcatggttg gttgtcaaca aagaagggaa acttgaagtg acttcaacag
    27421 caaaccaaga cacaccaatc tcagaaggta aaaaaccaat cttgggcttg gacgtttggg
    27481 aacatgctta ctacgtgaaa taccgcaacg tgcgtcctga ctatatcaaa gctttctttt
    27541 cagtaatcaa ctggaataaa gtagatgaat tgtacgcagc tgctaaataa tgatagttgg
    27601 agggaagaat tgttcttctc ttttttaggt tataagattc tggtctgaca aaatcgtcag
    27661 acttttttca tttttatgag aaacgtgcta actgctagaa attatggtag aataaagtat
    27721 taagtaatcg tggaaaaagg atatctatgc gaaaagaaat tgcacctgaa ttatacaact
    27781 ataacaagtt tcctggtccg gagttccatt tacacgggga caaggtcgaa acggaaggga
    27841 tagctttttc cttggttgaa aatatcaagg atgcctttga tgtgacgact tttaatcagc
    27901 gtttttcaga agtattaacc aagtttgatt atatcgtggg ggactggagc aacgaacagc
    27961 ttcgcctacg aggtttttac aaggatgacc gaacagaaga aaaacttgaa aaaatcagtc
    28021 gtttacaaga ctacctttta gagtattgta gttatggttg tgcctatttt gtcttagaaa
    28081 atgaagcccc taagcgagta tcatttgaca agaaaatgcg taagacggaa gaagaaacac
    28141 cttctagaaa aggaaagaaa ccgactcaaa ctaaacgaaa gtcgaatgca gataagaaaa
    28201 atagacgtcg tcagaaagac caacattctc agaaagagga caaggaacaa cgtcattttg
    28261 tcattcgtca gaagtgagta gagaataagg agaagaaatg aaaccctcaa ttcatagttt
    28321 ggcacatcaa accatgcagg aatgggtatt ggagcaggga gaaaagaaat tccgtgcaga
    28381 tcaaatctgg gaatggctct accgtaaacg tgtgcagtca tttgaagaaa tgaccaacct
    28441 ttccaaggat ttgattgcta agctcaatga ccagtttgtg gtcaatccct tgaaacaacg
    28501 tattgtgcaa gagtctgctg atggtacagt caaatatctc tttgaattgc ccgacggtat
    28561 gttaattgag actgtactca tgcgtcaaca ctatggttta tctgtctgtg tgaccactca
    28621 ggtcagctgt aatatcggtt gtaccttctg tgcctctggt ttgattaaga agcagcgtga
    28681 cctcaataac ggtgaaatcg tagcgcaaat catgctggtt cagaaatact ttgctgatga
    28741 aggggttcag gtcaatcttg ccgtgtctct tcacgcaccc aacaatgaac ttcgttcaag
    28801 tatcatgaaa attaaccgtg cctttccgat tgaaaaactc tttgctgcta ttgaatacta
    28861 cattgagaca acaaaccgtc gtgtaacctt tgaatatatc atgcttaatg aagtcaacga
    28921 cggtgtagaa caagccttgg aattggctga attgctcaag aacatcaaga aattgtctta
    28981 tgtaaacttg attccttata acccagttag tgagcatgac caatatagcc gtagtcccaa
    29041 agagcgcgtg ttggctttct atgatacgct taagaaaaaa gggggcaact gtgtggttcg
    29101 tcaagagcat ggtacagata ttgacgcagc ttgtggacaa ttgcgcttta acacaatgaa
    29161 acgtgaccgc cagaaggcag tcgcagcggt aaatccttaa tgatgaagaa gacttataat
    29221 catattttgg tctggggagt cattttctat agcatttgca ttgtctattt ttgctttact
    29281 cctcaagaac aatctaccgt gggagtggga actccagata ttcagcctct tggacgcctg
    29341 gtttttcttt tgactccttt caattctctc tggaaactgg gcgaagtgag tgacattgga
    29401 caattatgtt ggattttttt acaaaatatc ctcaatgtct tcttgttttt tcctctgatt
    29461 ttccaactcc tttatctatt tccaaatttg cggaaaacaa aaaaggtcct tctttttagt
    29521 tttcttgtga gtcttggaat cgagtgtacg caattaatct tggacttttt ctttgatttt
    29581 aatcgcgtct ttgagattga tgatttgtgg accaacactt tgggtggcta tctggcttgg
    29641 ctcctttata aacgattaca taaaaacaag ataaggaatt aaaatgagta ttttagaagt
    29701 taaaaatctg agtcacggtt ttggtgaccg tgcaattttt gaagatgtgt ccttccgtct
    29761 cctcaaggga gaacatatcg gcctggtcgg tgccaatggt gaaggaaaat caacctttat
    29821 gagtatcgtg actggtaaaa tgctgccaga tgaaggaaag gttgagtggt ccaaatatgt
    29881 gacggctggt tacttggatc agcactctgt ccttgctgaa agacagtcgg tgcgtgatgt
    29941 tctccgtacg gcttttgatg agcttttcaa agctgaagct cgtatcaatg acctctatat
    30001 ggaaatggct gaagacggtg cggatgttga tgctctcatg gaagaagtgg gagaacttca
    30061 agaccgtctg gagagtcgtg atttctatac cttggatgct aagattgacg aagtagcgcg
    30121 tgctcttggt attatggact ttggcatgga tacggatgta acttctttgt caggtgggca
    30181 aagaaccaag gtgcttttgg caaaacttct ccttgaaaag cctgatattt tgctgttgga
    30241 cgagccgacc aactacttgg atgctgagca tattgattgg ctcaagcgct atctccaaaa
    30301 ctatgagaat gcctttgtcc tcatttcgca cgatattcca ttcctcaatg acgttattaa
    30361 tattgtctat catgtggaaa atcaacagct gacgcgttac tctggtgact actaccagtt
    30421 ccaagaagtt tatgctatga agaaatctca gctagaggca gcctacgaac gccagcagaa
    30481 agagattgca gacctcaagg actttgtggc tcgtaataaa gcccgtgttg caactcgtaa
    30541 tatggctatg tctcgtcaaa agaaattgga taagatggat attatcgaac tccaaagtga
    30601 gaaaccaaaa ccatcctttg atttcaaacc agctcgtaca ccagggcgct ttatcttcca
    30661 agccaagaac ttgcaaattg gttacgaccg tcctcttact aagcctttaa atcttacctt
    30721 cgaacgcaat caaaaggttg cgattattgg tgctaatggt attggaaaaa caactctctt
    30781 gaagagtctc ttaggcatta tctcgccaat cgctggggaa gtggagcgtg gagattattt
    30841 agaacttggt tattttgagc aggaagtaga aggcggtaat cgccaaactc ctcttgaagc
    30901 tgtctggaat gcctttcctg cccttaatca agcagaagtc cgtgcagccc ttgcccgttg
    30961 tggtttgaca accaaacata ttgaaagcca gattcaagta ttatcagggg gagagcaagc
    31021 caaggttcgt ttctgtctct tgatgaatcg tgaaaacaac gttttagtgc tggacgagcc
    31081 gaccaaccat ttggatgtgg atgcaaagga tgagctcaaa cgcgctctca aagaatatag
    31141 gggatctatc cttatggtct gccacgagcc agacttttat gaaggctgga tagaccaaat
    31201 atgggatttt aataatttaa cttaagaatg agagtaaaag aaatacagta ccgaatgtgg
    31261 atactgtatt tttttggttt ttaagattta aaatcgtagt ctttcaccac ttcgatgctg
    31321 tcgattgtga tagcagtagt tggcttgtct ttttcatctt tttcggcctt agcaatctta
    31381 tccacaacat ccataccgtc aatcacttga ccaaagactg ggtgtttgcc atctagacta
    31441 gggtttccac cttctttgta ggcttcgata attttctgtg gatacttgct tgtagggagt
    31501 ttagaagagg tatctgtaga gttttggttg atgaagaact ggctgccatt ggtgtttggt
    31561 tgaccagtat tagccatagc aagagcacca cggatgttat acaaataagg agtaatctcg
    31621 ttcttgaaac cagttccttt gtctttagtc ttatccttgt catgccagat ggactgacca
    31681 cctgtaccgt ccccttttgg atctccagtt tggaccataa agccatcgat gacacggtgg
    31741 aaggtaatac cgttatagta gccttctttg gcgtgagtga ggaaattttc aaccgctaga
    31801 ggagcgagtt tagggaagag tttaatgcga atatcacctt ggcttgtgtg gagaatcact
    31861 tcggcttcat cttcagcaac ttccttagat agttgtggga aattggcgtt ttcgtttgtt
    31921 aaagcatcgt ttaactcctt ggcagattgg gcagctactt tggaactttc ttcagcagca
    31981 agactggaat caacataatc atcaccacgc agactgcgtt ggacgctgct acacccagct
    32041 agggctacag tagacagtaa aagaagggtt gctagttttt tcattctgat cctctcaaaa
    32101 attcatttat aaccattgta ccctaaaagg aagtggattt caaatattag tagtttacag
    32161 aactggctta gataatagtt gggatttcat catcatcttg aaaatcttca acttcttccc
    32221 cagtaaatgg tttctgaaaa gaacttagta taaaacgggc aattggttca acaattagga
    32281 aatttagagg aagagctgca ataaagttta cgaaccaggc gcttagatag gtattgggtg
    32341 tgatagcaat gccattgtaa aaaaagtcca tagaaggaca tgaaagtgac cagctaattc
    32401 ttcttctgaa aaatgctcat gaagggcttg atccaggtcg caagatttca ttttagaaag
    32461 tccagtaact cccttcttct tcaggatatt ctttttcatg gtagtattta gatggtcaag
    32521 tgagtcaaaa gaagtttcga caagagcata ccctttttct accagagttt ttagatgttt
    32581 aggggcatca ataccataag tgtactcaaa gtattttgga aaccagatct taagaaagct
    32641 cgtaaagcta gccagttctc taaacgctaa gaactagcta caacacagca tttacaacac
    32701 ttcatggttc acaccatggg gtgtttttta atgattttaa gcaaaattca cacttaattg
    32761 aaagttattt aaattagatg aaaatttatt ttttgaaaaa gtgggaaaac attgattcta
    32821 agcgaatttt gaatcatatt cgaatgggag tttttaaaat catgtttcag tggtaaaagt
    32881 accaaactgg attctccata gtaaaatgat atctcctgct agtgaatcat ctactaataa
    32941 gtccatattt cgcttgggaa cgggcttggg acttagaagc caagtatcca actctcgttc
    33001 aggcgatatg aagggtttga cttggtgttc tttataaaat ttttctaaaa tagtattcac
    33061 ggatagtatc tttcaaacat ttatttccta ttctaa
//
LOCUS       DAVLFB010000012        32977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLFB010000012 DAVLFB010000000
VERSION     DAVLFB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.012"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(256..1818)
                     /gene="guaA"
                     /locus_tag="VXD58_000500"
     CDS             complement(256..1818)
                     /gene="guaA"
                     /locus_tag="VXD58_000500"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV0675247.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CLYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1960..2658
                     /locus_tag="VXD58_000501"
     CDS             1960..2658
                     /locus_tag="VXD58_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0675248.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2701..3597)
                     /locus_tag="VXD58_000502"
     CDS             complement(2701..3597)
                     /locus_tag="VXD58_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0675249.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3606..4541)
                     /locus_tag="VXD58_000503"
     CDS             complement(3606..4541)
                     /locus_tag="VXD58_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV0675250.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4538..5263)
                     /locus_tag="VXD58_000504"
     CDS             complement(4538..5263)
                     /locus_tag="VXD58_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV0675251.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5347..5982)
                     /locus_tag="VXD58_000505"
     CDS             complement(5347..5982)
                     /locus_tag="VXD58_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV0675252.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5984..6811)
                     /locus_tag="VXD58_000506"
     CDS             complement(5984..6811)
                     /locus_tag="VXD58_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0675253.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6833..7168)
                     /locus_tag="VXD58_000507"
     CDS             complement(6833..7168)
                     /locus_tag="VXD58_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV0675254.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7169..7885)
                     /locus_tag="VXD58_000508"
     CDS             complement(7169..7885)
                     /locus_tag="VXD58_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV0675255.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            8398..9009
                     /locus_tag="VXD58_000509"
     CDS             8398..9009
                     /locus_tag="VXD58_000509"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0675256.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9054..9782
                     /gene="rlmB"
                     /locus_tag="VXD58_000510"
     CDS             9054..9782
                     /gene="rlmB"
                     /locus_tag="VXD58_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV0675257.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9821..10732)
                     /gene="trxB"
                     /locus_tag="VXD58_000511"
     CDS             complement(9821..10732)
                     /gene="trxB"
                     /locus_tag="VXD58_000511"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV0675258.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10798..11022)
                     /locus_tag="VXD58_000512"
     CDS             complement(10798..11022)
                     /locus_tag="VXD58_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV0675259.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11100..11843)
                     /locus_tag="VXD58_000513"
     CDS             complement(11100..11843)
                     /locus_tag="VXD58_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0675260.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11843..12643)
                     /locus_tag="VXD58_000514"
     CDS             complement(11843..12643)
                     /locus_tag="VXD58_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0675261.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12801..13157)
                     /locus_tag="VXD58_000515"
     CDS             complement(12801..13157)
                     /locus_tag="VXD58_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV0675262.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13170..13682)
                     /locus_tag="VXD58_000516"
     CDS             complement(13170..13682)
                     /locus_tag="VXD58_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV0675263.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13682..14176)
                     /locus_tag="VXD58_000517"
     CDS             complement(13682..14176)
                     /locus_tag="VXD58_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675264.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14311..14757
                     /locus_tag="VXD58_000518"
     CDS             14311..14757
                     /locus_tag="VXD58_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV0675265.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14754..15401
                     /locus_tag="VXD58_000519"
     CDS             14754..15401
                     /locus_tag="VXD58_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV0675266.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15422..16003)
                     /gene="pdxT"
                     /locus_tag="VXD58_000520"
     CDS             complement(15422..16003)
                     /gene="pdxT"
                     /locus_tag="VXD58_000520"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV0675267.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16004..16879)
                     /gene="pdxS"
                     /locus_tag="VXD58_000521"
     CDS             complement(16004..16879)
                     /gene="pdxS"
                     /locus_tag="VXD58_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV0675268.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17031..18410)
                     /locus_tag="VXD58_000522"
     CDS             complement(17031..18410)
                     /locus_tag="VXD58_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV0675269.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18704..19627
                     /locus_tag="VXD58_000523"
     CDS             18704..19627
                     /locus_tag="VXD58_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV0675270.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19687..20292
                     /locus_tag="VXD58_000524"
     CDS             19687..20292
                     /locus_tag="VXD58_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0675271.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20311..21555
                     /locus_tag="VXD58_000525"
     CDS             20311..21555
                     /locus_tag="VXD58_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0675272.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSS
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21778..22035)
                     /locus_tag="VXD58_000526"
     CDS             complement(21778..22035)
                     /locus_tag="VXD58_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV0675273.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22077..24113)
                     /locus_tag="VXD58_000527"
     CDS             complement(22077..24113)
                     /locus_tag="VXD58_000527"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV0675274.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24373..25290)
                     /gene="glyQ"
                     /locus_tag="VXD58_000528"
     CDS             complement(24373..25290)
                     /gene="glyQ"
                     /locus_tag="VXD58_000528"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV0675275.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(25309..26070)
                     /locus_tag="VXD58_000529"
     CDS             complement(25309..26070)
                     /locus_tag="VXD58_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0675276.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26343..27185)
                     /locus_tag="VXD58_000530"
     CDS             complement(26343..27185)
                     /locus_tag="VXD58_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV0675277.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLESLKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTTAVQSGAPRVD
                     DVDF"
     gene            complement(27300..28691)
                     /locus_tag="VXD58_000531"
     CDS             complement(27300..28691)
                     /locus_tag="VXD58_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV0675278.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(28926..29303)
                     /locus_tag="VXD58_000532"
     CDS             complement(28926..29303)
                     /locus_tag="VXD58_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0675279.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            29518..30495
                     /locus_tag="VXD58_000533"
     CDS             29518..30495
                     /locus_tag="VXD58_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0675280.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30492..31574
                     /locus_tag="VXD58_000534"
     CDS             30492..31574
                     /locus_tag="VXD58_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0675281.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31638..32668)
                     /locus_tag="VXD58_000535"
                     /pseudo
     CDS             complement(31638..32668)
                     /locus_tag="VXD58_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         9785 a         7359 c         5908 g         9925 t
ORIGIN      
        1 ctccatgaat gatagatacc ttgttatgac gcgcttacta tattagaaag ttggctctct
       61 aattaaatta attctcatta tatatatcaa aagaccgcag tttgaaaact acggtctaat
      121 tttttgtcca aatgtaaatc acttggaact attattcaat ttattctttt tagtaaatgt
      181 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
      241 ttaatttttt tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta
      301 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
      361 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
      421 tgcaatcgtg tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
      481 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
      541 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
      601 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
      661 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
      721 ttcaaactgc atatcttctg gaagaccacc tacgttgtgg tgtgacttga tagtttgagc
      781 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
      841 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
      901 acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
      961 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
     1021 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
     1081 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     1141 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     1201 ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta
     1261 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     1321 gatggctgcg tataggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     1381 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     1441 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     1501 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     1561 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     1621 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     1681 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     1741 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     1801 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta
     1861 tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt
     1921 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     1981 atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg tgagaggctt
     2041 cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc
     2101 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     2161 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     2221 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     2281 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     2341 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt
     2401 aaggacttta aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc
     2461 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     2521 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     2581 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt
     2641 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     2701 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     2761 cataccaata aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaac
     2821 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     2881 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     2941 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     3001 gaccaaacct gctatgacca tccccacacc aatgaccaag ctgctccccc actttttaat
     3061 caaggctatg ggtaaaatga tatacaaagc ataagtcaag gcagaaaaga gaccccagaa
     3121 caggccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     3181 tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     3241 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     3301 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     3361 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     3421 ctttgcagta gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt
     3481 ggtcaagacc agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa
     3541 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     3601 tctccttact cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga
     3661 gctctctcgt ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga
     3721 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     3781 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt
     3841 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     3901 aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     3961 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc
     4021 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     4081 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     4141 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     4201 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct
     4261 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     4321 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca
     4381 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     4441 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     4501 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     4561 caacattatt tctatcttta caaaggaaga atttttcaga cttaggaata aaatattcag
     4621 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     4681 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     4741 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     4801 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     4861 caggcttttc tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca
     4921 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     4981 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     5041 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     5101 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     5161 acaaggcctt cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag
     5221 cttttaaagt aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt
     5281 tttattttaa aggattttag aattttttac aagatattat gctcaaattt aacattattt
     5341 agctatttag taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact
     5401 gaggtgcagt ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca
     5461 atcaaatact ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg
     5521 attccagttc tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct
     5581 ctcattgaca ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac
     5641 ggattgaatg atagcttcga gattattgag agcctcattc acaggaacat cctttccgat
     5701 atcatttgtc ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc
     5761 aaggttctct agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt
     5821 tgaagtccca aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag
     5881 aatgtttggt tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc
     5941 ttgttccttt aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta
     6001 accagttttc caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg
     6061 ccacagcttt ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa
     6121 gcatttcaac atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc
     6181 agcgcttgag taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa
     6241 tgcaaccata gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt
     6301 ccaaaaccga actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa
     6361 agaggtcaga tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt
     6421 ctggagtcat aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct
     6481 tcatccccgt tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct
     6541 tgttgacaat ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac
     6601 gagcgccatt agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca
     6661 tgcggtgaat ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat
     6721 ctaaaatctt ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat
     6781 ccatatctgt cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc
     6841 acttcaatcc cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg
     6901 cttcatctaa ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt
     6961 ggtaatgata caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc
     7021 aagtaggatg agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga
     7081 catccatgag aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa
     7141 ttcgttgatt ggaaatgaag ttccccattt aatagacaat gagcttttta tctccatctt
     7201 tttccaccgt ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc
     7261 cccaatcaat catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact
     7321 caacacccat ctgaggcatg ataatggtga tatctgcttc cttctctgcc cgttcaactt
     7381 cagcctttat cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa
     7441 tatactgctc aattccattg aaaccatagg aataggctaa caatgcaacc ttgatagcat
     7501 tcacttcctt aatgaccagc ggagcctgag cacgtggttc gtgcgtataa actccgattg
     7561 gagtgattcc agctttctca ataatatcgg ccattgaaat aactccctca atttgcgaat
     7621 ccaaaatatg attatgagct aaatccagca catgataacc tgcctcctta atagcatcca
     7681 taacttcaac aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg
     7741 ttccttcaaa atcaccaata gctaaatctg cttgcttgag ccaaggagtc acatactcaa
     7801 aattttcatg aaagtcatag gtaccgtctt cttttttagc tgaaaagaaa agtccatcgt
     7861 ggtagagcaa atccccgtgg gccataattc tggcagtttt ttcctcttcc tactcctgag
     7921 acttttgctt agtcccctct tgagaaatag tatctcgttt ttgactagtc aagggattcc
     7981 ttggaagctt tcaaacaaca agaccaagcc cattgataaa ccaactgcta gcaagagtat
     8041 cgccacaaat cccttattgc tccacttgcg ataactccta aaaagtttac caagcccttc
     8101 ataaaacgaa aagctgaacc accctgattt cgattttgtc ttctttgtat cttcgttctc
     8161 cctactttct tatgcaagcc ttttcttttt attatatcac agataagtat ttctttcaca
     8221 attgaattga acttcctatc tattttctat aaatcctaaa tgccataatg ctttcaattc
     8281 ttgtcatttt gtgatatcat gtagaggaaa tgaactaatc cacagtggct tattccaagt
     8341 ataccacttg ggctttggca gtagctaact gcactaaaca taatataagg agagaaaatg
     8401 tctgcaatag aacgtattac aaaagctgct cacttaattg atatgaacga tattatccgt
     8461 gaagggaatc ctactctacg cacggttgct gaggaagtca ctttccccct atctgaccag
     8521 gaaatcatcc taggcgaaaa gatgatgcaa ttccttaaac attcccaaga tcctgtcatg
     8581 gctgaaaaaa tgggactccg cggtggtgtt ggactggctg ctccccagtt agatatctca
     8641 aaacgcatta tcgctgtttt ggtacctaat attgttgaag aaggcgaaac tccacaggaa
     8701 gcctacgatt tggaagccat tatgtacaat ccaaaaatcg tctctcactc tgttcaagat
     8761 gctgctcttg gcgaaggaga aggttgcctg tctgttgacc gtaacgtgcc tggctatgtt
     8821 gttcgccatg cccgcgttac tgttgactac tttgacaaag atggagaaaa acaccgtatc
     8881 aaactcaaag gctacaactc cattgttgtt cagcatgaaa ttgaccacat taacggtatc
     8941 atgttttacg atcgcatcaa tgaaaaagac ccatttgcag ttaaagatgg tttactgatt
     9001 cttgaataaa gaaaatcccg ttgcaagacg gggttttgtg ttataataga ggcatgaaaa
     9061 caaatgatat tgtctatggt gtccacgctg ttaccgaagc cctccttgca aatacaggaa
     9121 acaaactcta cctccaagaa gatctccgag gtaagaatgt tgagaaagtc aaggaactag
     9181 ctacagaaaa gaaggtgtcc atttcttgga catcaaaaaa atctctctct gagattactg
     9241 aaggtgctgt tcatcaaggt tttgttctac gagtgtctga atttgcctat agcgagctag
     9301 attacatcct tgcaaaaaca cgccaagaag aaaatccact tctattgatt ctagatggtc
     9361 taaccgatcc ccataatctg ggttctatct tgcgaacagc cgatgcgacc aatgtttcag
     9421 gtgtcatcat tcccaagcac cgtactgtcg gagtaactcc tgtcgttgcc aaaacagcca
     9481 caggtgctat tgaacacgtt ccaattgccc gagtgaccaa cctcagtcaa accttagata
     9541 aacttaagga tgaaggtttc tggacctttg gaacggatat gaacggtact ccttgccaca
     9601 agtggaatac aaaagggaaa atcgccctca tcattggaaa tgaaggaaaa ggtatctcta
     9661 gcaacatcaa aaaacaggtc gatgaaatga ttaccattcc gatgaatgga catgttcaaa
     9721 gccttaatgc cagtgttgct gcggccattc tcatgtacga agttttccga aatagactat
     9781 aaaaaagttt ccagtcatct gattggaaac ttttttatga ttaactatgt tctgtaatga
     9841 atttataggc ttcttgacca gcgatagctc catctccaac cgctgttgtt acttggcgaa
     9901 ggtctttcaa gcgaacatct ccaactgcaa agataccgtc gactgcagtt ttcatgtggt
     9961 tatctgtcac aatccatcct gcctgatctt ggatattcaa ttctttaaca aaatcgctaa
    10021 gagggtccaa accaacatag ataaagacac caccgaaggc ttgttctgtc acttgacctg
    10081 ttttcacatt ttcaaatacg actgattcta ctcggttttc acccttgatt tcccttacta
    10141 cagaatccca gataaagctg attttttcat tcgcaaaggc gcgatcttgt aaaacctttt
    10201 gggcacgaag ttggtcacga cggtgaacaa tggtaacagt cttagcaaaa cgagtcaaga
    10261 agagggcttc ttcaacagct gaatctccac caccaactac caataaatct tggtcacgga
    10321 agaaagcacc atcacacaca gcacagtaag aaacaccacg actgttcagt tcttcttctc
    10381 caggcactcc caaaggacgg tgtttagaac cagttgctac gataactgta cgtgtttcat
    10441 atgtttggtc atcagtcatc actttcttaa aatcaccatg gtcttcgaca ttttcaacat
    10501 aaccataaat gtgctcaaca ccaagatttt caagtggttc aaacatcttt tcagccaatt
    10561 caggtccact aatattagcg tatcctgggt aattttcgat atcagatgta ttattcatct
    10621 gaccacctgg cagaccacct tcaatcaaag ctacttttag attgcttcga gcagcataca
    10681 aggccgcagt catccctgca ggtccagcac cgataataat agtatcgtac atatagattc
    10741 cttctttctt gttgtaacta tctttattct aactctttct tgtcaatcac gcaaggatta
    10801 tgccttgaaa acaagaatta aactcataac cgcaaaggat aatactggga caaccaagac
    10861 tccaatcata atcatatcat agagatgggc ttggcgagcc ctggctgtct tatcaactcc
    10921 cgacatggct ctcagaccaa tccaaatccc taaaaaaatc agaacaagga tggtggtcaa
    10981 gatcaaactc tcgaaatata aagaaaatag ttgcagtagc atgatttctc tcatttctat
    11041 cttttttaaa gagtaaactc agctagtcca actaactgag ttttccttta tctattatat
    11101 caaatataag tccgtttgta actagcgaag aattcttttg tccgctcttc tttaggggtg
    11161 tggataatct catccggagt tccagactcg atgattttcc ccttatctaa gaagagaatt
    11221 ttatccgcaa cttgggctac aaaggacatg tcatgactga ccaaaatcat ggtctgacct
    11281 gacttagcag catctgcaat agacttttct acttcaccga ccaattctgg gtcaagggct
    11341 gaagttggtt cgtctaagag caaaacatct ggtttcatag caagcgcacg cgctagggca
    11401 acccgttgct tctgtccacc tgataaatgg cgaggataat ggttttcacg gtccgaaagc
    11461 ccaaccttag ccaactcttc cttggcaatc ttagtcgctt cttggtcaga taatttcttg
    11521 acaacaacca agccttcttt cacattatca agtgctgttc ggcgttcaaa taaattaaac
    11581 tgttggaaaa ccatagacaa cttacggcgt agggcaagga tttcttcttg agtgatttta
    11641 gaaaaatcaa ctgaaaaacc atcaatctga atagagccac tgtcaggtgt ttcaagataa
    11701 ttgagactgc gaagaaaggt tgattttcca gctcctgaag aaccaatcaa ggctacgact
    11761 tctccttttt gaatatccaa gttcagatga tccaagacag tctgtcctga aaaggatttg
    11821 cttaaattcg aaatcttaat cattaacgaa ggtctccttt cacatctgtt tgcactgtat
    11881 caggtgcaga aatagccatt tttctctcga tgaaacgacc gaggctttca attccgatat
    11941 tgactaccca ataaacaagg gcaacggaga tgaagcgttc aaaatagcga taatcagctc
    12001 cacctagaat ctgagcttgg gcaaagactt ccacaacacc cgcactaaaa gctagagatg
    12061 ttcccttggt caaaccgatg agggaattaa tcaaggttgg agtagctacc accgctgcat
    12121 taggaataat cactcgtcga taaacttgcg ctcgggtcat acccagactg cgtgccgcct
    12181 caatctcacc aggattaact gagagaatgg ctgcacgaat ggtttcacta gcataagctg
    12241 cctcattaaa ggcaaaagcg acaatcgcaa aagctgcagc tggaatcgca ttgatattga
    12301 gaccagttcc ccattgctga ttgagggctt tcaaagccaa agggattccg tagtaggtca
    12361 acatgagttg caccaaaatc ggtgtccctt ttaagaaact aacaaagaag gcctgcaagg
    12421 gatataaaat cttgacacga ttgatcttca caatggcaaa aagaagcgcc aaaaccaagc
    12481 caaaaagggc accaccaatt gtcaacataa ttgttgttgg aagttgttgg acaattctag
    12541 ggattccatc aaagaccgaa cgtaggctaa acagcttgcc atccggaatc aattgcatca
    12601 agttttggta ccaatctgat gctaaaatcg ttgtaacatt cataaaaaca tcctcttctg
    12661 ataatgattc attctaccat gcttctgccg tcaatgaaat tatttgctac ggtcagatac
    12721 agactttgtc tacctactag tttatcatac tttgaaacag ggaagttata tattttatct
    12781 atcaaagaga tagataaaaa ctatttcagt cccagttctt cataagattt tcgataaccg
    12841 atttgcttaa cagttccatt ttctactaaa atgggccgtt ttaacaacat accgtcactt
    12901 gctagcaact cagccgcttc ttggtttgac aaacttccta ccttatcttt tagccctaat
    12961 tcacggtatt tgataccact ggtgttgaaa aattgcttca attcaaatcc tgaggtttct
    13021 agccaattca aaatgacttc ttggctaggt gtttcttcca cgatatggac ggctttatag
    13081 tccacaccta attgatttaa ttcttgtttt gcttttttac aagttgaaca tttggggtat
    13141 tcgataaatt ctaacatgtg cttctccttc tatttgttta tatctttaaa atctacgcct
    13201 tcatgctcca agagccaagc tttcttttcc actcctgcag catagcctgt cagacgcttg
    13261 cctgctccca acacacgatg acaaggtact aggatagacc aaggattgcg tcccactgct
    13321 ccaccaattg cttgagcaga agccacttgc aggtcttgag caatttgtcc ataggtcact
    13381 gtctgaccat aaggaatgcc ctgtaaatag tcccaaactc tcttttcaaa attcgttccg
    13441 attggcgcca agagcaagtt ggataaatcc tgaggcttgc ctttaaagta atcatctaag
    13501 caagcaataa ctgggtctaa aataggatga ctaacaactt cttctatcgt ttcatctcct
    13561 agtcccctct caaaatgctt ctgctcctga acccaaattc catacaaata atggtcatca
    13621 gctacaagtg atagaatacc aattggtgag gagtagagta tttttacata cttcttttgc
    13681 attatttctt taatttttga taggccaagc gttcgccatc tactggcacc ttaccaactt
    13741 gtttaaaacc aagtttttca aaaatatgtt gcataacctt gttttcagca tgcgtatctg
    13801 agcgaaaatc aagataatca aaaccttcaa tcaagccctc taagaaggtt tgagcaactc
    13861 cttttccctg cacatctgct gccacagcaa tacggtgaaa gactagatac tctgactctc
    13921 cagcttgcca gtttccctca taaatagctt cataggcctc ctctggactc ttggtcacag
    13981 cagcataggc tagcagttct ccctcttcca aggctacgta ggcttgccct gagatgatat
    14041 catcgataat aacatctgca tttggatagc cattttgcca ctggtcacta ccagcatctg
    14101 ctaaacattt tttggcatcc tccatcacct gcatgatggc atctacttca tttggaaaag
    14161 ctaaacgaat ttccatcttt tttcctcatt tctgactagt ttctcccatc atagcataat
    14221 ttaagtattt ctacaagcag ttaaaacttg actttgtgtt tttgttattt tataatctag
    14281 ttatcaaaac tatataaaaa ggagttaaaa atgaaaacta ccttttccta cccaaaatgg
    14341 gcagaaattc caaacattga cctctatctg gaccaggttt tgctctatgt caatcaggtc
    14401 tgcgccccta tctctcctaa taaagacaag ggcctaacag catctatggt caacaattat
    14461 gtgaaaaatg gttacctgac aaagcctgac aaaaaaaaat accaacgcca acagattgcc
    14521 cgtttgattg ctatcacaac cctcaagtct gtattttcta ttcaagaaat agcccagaca
    14581 cttaatactc tacaaactca agcaagttca gaccaactct acgatgcttt tgtggactac
    14641 atgaaccaag gaattgaccc agctaaccct attatccaaa ccagctgcca aaccgttaaa
    14701 ctctatcatc aaactctaga cttaatcgat catactcaag aggaggtaat ccaatgaaca
    14761 ctagtcttaa actcagcaaa caactcagtt ttggagagga gattgctaat agcgtgaccc
    14821 atgctgtggg tgcagtcatc atgcttatct tgctgcctat ttcatccatc tatagttatg
    14881 aagcacacgg atttttatcc tctatcggcg tttccatttt cgtcatcagt ctctttctca
    14941 tgttcctatc atccaccatt tatcactcta tggcctatgg ttcgacccac aaatatgttt
    15001 tgcgaatcat tgaccattct atgatttacg ttgccattgc cggatcatac acgcccgttg
    15061 tcttgacctt gatgaataac tggtttggct atctgattat tgtcatccaa tggggaacga
    15121 ccatctttgg tattctctat aaaatctttg ctaaaaaggt caatgagaaa tttagccttg
    15181 ctctttacct gattatgggc tggttggttc tggctatcat tcctgccatt atcagtcaaa
    15241 cgacacccgt tttctggagt ctcatggtaa ctggcggact ctgttataca gttggagctg
    15301 gattttatgc caagaaaaaa ccttatttcc acatgatttg gcatctcttt atcctagctg
    15361 cgtccgcact tcaatacatc gctattgttt attacatgta aaaaagttga gaaattcaat
    15421 ctcaactttt ttctttacac atattgataa agtactggtg caagcgcaca tcatcagtca
    15481 attctggatg aaaagaactt accaacatat ttttttcttg ggctgcaaca atttgattgt
    15541 tcactgttgc taaaatttct acaccctcac caacactact gataatcgga ccacggataa
    15601 aggtcattgg aatcttgcca actcccttac attctgcttc cgtgtagaaa cttcctaatt
    15661 ggcgcccata agcattacgc tcgaccacca tatccatagt tcctagatga ctctctttct
    15721 gagaagtgat ttccttagcc agcaaaatta agcccgcaca ggtcccaaac actggtaagc
    15781 cagatagaat ggcttctcgg atgggaagta gcatgttctg gtcacgtaag agcttgccca
    15841 tggttgtaga ctcaccacca ggcaaaatca aacccgacaa gtcactctga tcttgctgaa
    15901 aatcatctag atttctgagt tctacactct cgacacctaa ttgatctagc acttttgcat
    15961 gttctgcaaa ggccccttgc aaggccaata ttccgatttt catctatttt cctcgttcag
    16021 ccatgagaat ttggatttca ttttcattaa taccaaccat ggcttctcct aaatcttcag
    16081 agatttgagc taggatttga ggattacgga agttagtcac agccttaaca atggcactcg
    16141 ctcgtttaac aggatctcct gacttgaaaa tacctgaacc gacaaagacc ccctctgccc
    16201 ctaattgcat cattaacgca gcatctgctg gcgttgcaac acctccagca gcgaaattta
    16261 caactggcaa ttttccatgt tcatgaacat attggaccaa ttctacaggg acttgcaaat
    16321 ccttggcagc aacataaagc tcgtcctcac gtaagttttg aatgcggcga atttcctgat
    16381 tcatcatacg catatgacga acagcttgga cgatatcccc tgtccctggt tctcctttgg
    16441 tacgaatcat ggaagcacct tcagcgatac gacgcaaggc ttcacccaaa tccttagcac
    16501 cacagacaaa aggaacttgg aattctttct tgtccacatg gaaacggtca tcagctggag
    16561 atagaacttc actctcgtcg atataatcaa tttcaatagc ctctaaaatc tgagcttcaa
    16621 caaaatgccc gattctgacc ttagccatta ctggaatact aaccgcttct tggatttcct
    16681 taatcatctt tgggtcgctc atgcgggaaa ctcctccagc tgcacgaata tcagccggaa
    16741 ttcgttccaa ggccatcaca gctgccgcac cagcagcttc tgcgatacga gcctgttcag
    16801 gattctgcac atccataata acaccaccct tgagcatctg tgccaagttt ttatttagtt
    16861 cataacgatt ttcagtcatt tgttttatcc tcatcatttg tattggtagc taccttttca
    16921 ttttaagtca gaacagaatg aagcacaaga taaaaaaact ataattgtat ggggacaaat
    16981 tagctaaaaa ctatctgacc ttaacttaag gccagatagc tcattcattt ttatttttca
    17041 gccgtaaggg cagccattgt gatgtagttg tatggtttgt tgaagtgtgg caagaagaag
    17101 aggtctgtca atgccaattt atcaattgtc acatgctctt ggatagcaag tgagaacatg
    17161 tggattccca tgctaattgc aatatcatgt gaaaccattt gggcaccaag aatttcacgg
    17221 ctatctttgt caaagacaat cttaattgct acttcatggt tgtcatgttt catgaattct
    17281 ggtttttgaa gatcgttaaa gcctgtttca gttgcgttgt aaccagcagc tttcgctttt
    17341 tcaagagtca aaccagttga aaccatgtga agaccgtaga ttgagatacc atttgaacct
    17401 tgaacaccga ttccttccaa ttcatgtcca caagcattgt aggcaccaac gataccagtg
    17461 cgaacagcat ttgaagcaag agcgatatag cttgtatctt tacgagcatt gtcataaaca
    17521 gtcgcacagt caccaacagc gtaaacgtct gggatagatg tttcttgttt cttgtctaca
    17581 aggaaggcac cgttgcggaa gagttcgatc ttaccacctg caagggctgt gtttggacgg
    17641 aaaccaactg caaggataac catatccacg tcaaagcttt ctttgtcagt aatcaagcgt
    17701 tcaactttac cgtcaccttc gattgcttta acagtttgac ctagagccaa gcggatgttg
    17761 tgatcttcca agttcttcgc catcatttgt gtgaagtctt tgtcatagta accgttcaag
    17821 acagtatcaa cgatatcaac aaggacaact tcttttccaa gacgttcaaa ggcttcagca
    17881 agttcaacac cgatgtaacc accaccaaca acggcgatac ggtcgaggtg ttggctcttg
    17941 tcagaaagtt tattgataac ttcttcagca ttttggtaca atttcacgaa ttgtacgttt
    18001 tcaagagttg ctttaaattc gcggtttcct ttaacaattt caacaccttc gattggtggc
    18061 aagattggtg tagaacctgt agcgaaaatc aatttttcgt atgattcttt gtgctctttt
    18121 ccttcaactt ccgctgtaac tactttgtta tcatagtcga ttgaaagaac aggtgagttc
    18181 atgtaaactt tagcaccttt agcttccaat ttttctttat cagaatagaa caagccttca
    18241 gcaccgtcaa tttgttcacc aatccaaaga gccattccac atcctaggaa agagatgtta
    18301 gagttttggt caaatacaac aatttcgttc tcatttccaa aattatccaa catggtattg
    18361 atacatgctg taccagcgtg gttagcaccg actacaacga ttttactcat aaaaatttcc
    18421 tgccttcatt ctaaatttac cttactagta taccatttac cgttagcgtt tacaaggggg
    18481 gtactattat ttcagcattt tttatcaaaa ataattcccc atttatcctg ttttcaagcc
    18541 tgatgaattt tatgagcata tttcttgact ttttccaaat tttgtttgtg aaaattctaa
    18601 ctttatggta ttataataac atgaaaatga aatgtaaggg cttcaattta gatgattatt
    18661 tgatattaca acttattttc tataagagaa aggagttggt agcttgactc ttcgttcaca
    18721 ttctgaacgg ctaatgggga ctactatcac tatttcatta gtggatgagc aagccgatat
    18781 ctttctccaa aaatcctttg acttgctcaa agaacttgaa taccgcttca acgccaatag
    18841 tcaagaatct gagttgatgg aaatcaatta tcaagcagga atagcccccg tcacggttca
    18901 tccagacctg tttgagctga tttcacttgg gttagagcat agcctagcgc tctctagcca
    18961 cctcaatatc agcattggtc ccttaattca aacctggcgt atcggttttt cagatgccaa
    19021 ggtcgcccag cctcaagaaa ttgaatcggt gctgcctcta atcaatcctc atggtatcga
    19081 gttagattct tctacttcta ctgtgttttt aaaacagaaa ggaatgaaga tcgatctagg
    19141 ttgtttagcc aaaggataca gtgcggataa ggttgcccaa tttcttagga aagagggggt
    19201 gacttctgcc ttgatcaatc tgggagggaa tatcctgacc attggaaaaa atcaggcaag
    19261 aggggataac ccatggcaaa tcgggattca agacccagcc aatcctaggg gaaatcattt
    19321 aatgaccatc cctgttgtca ataaatctgt cgtgacttca ggcatttatg aacgtcacct
    19381 gaccgtcgat ggacaagatt accatcacat ttttgacagt caaacaggat atcctgttga
    19441 aacggaacta gcgagtctaa caatcatctc tgataaatca gtcgatggcg aaatctggac
    19501 gactcgactc tttggagaaa gacctgcttc tatcctctgg caagtcgaaa gtttggaggg
    19561 catcgaagtc atcctcatcg ataaagaagg ccacctaagc tgttcttcag gaattcctac
    19621 tctatagaga caactgtttc aatggaattt taaaattgta ttatgtaaaa agagaaagga
    19681 aatctcatgt taaaacttat tgctattgtt ggaacaaatt caaaacgttc tacaaaccgt
    19741 caattgcttc aatacatgca aaaacacttt actgacaaag ctgaaattga acttgttgaa
    19801 atcaaggcca ttcctgtctt caacaaacca gctgacaagc aagtacctgc tgaaatattg
    19861 gaaattgctg ctaaaatcga agaggcagat ggcgttatta tcggtactcc tgagtatgat
    19921 cactctattc cagctgtttt gatgagcgct cttgcttggt tgtcttatgg tatttaccca
    19981 cttttgaaca aaccaatcat gattacaggt gcttcttacg gtacgcttgg ttcatctcgt
    20041 gcccaattgc aacttcgtca aatcttgaat gctcctgaaa tcaaggcaaa tgttcttcca
    20101 gatgaattct tgctctcaca ctctcttcaa gcatttaacc caagtggcga cttggttgac
    20161 cttgatgtta tcaagaaatt ggatgccatc tttgatgact tccgtatctt tgtaaaaatc
    20221 acagaaaaat tacgtaatgc acaagaatta cttcgcaaag atgctgaaga ctttgactgg
    20281 gaaaatttgt aagataggag accaaaaaga atgaaatttg ttggacttgt tggatcaaac
    20341 tacgatcaat catataaccg taagctcttg gaatttatcc gtcgctactt caaactcaaa
    20401 tttgaattag aagttcttga aattgacgaa gttccaatgt ttaaccaaga tgaaaaatgg
    20461 gatgaaagtt tccaattacg ttatttatat aacaaaatta ctcgtgcaga tggtgttatc
    20521 attgctactc ctgagcataa ccacactatc tcagcttcac tcaaatctgt acttgaatgg
    20581 ctttcatacg aagttcatcc atttgaaaac aagcctgtta tgattgtggg tgcatcatac
    20641 tatgaccaag gaacatcacg tgctcaagtt caccttcgta aaatccttga tgctccaggt
    20701 gttaatgcct acacgcttcc aggtaacgaa ttccttcttg gtaaagctaa ggaagctttt
    20761 gataacaacg gaaatatcac caacgaaggc actgttaaat tccttgaaac ttgcttagat
    20821 aactttgtta aatacgtagg agtcgtttcg aaattgaaaa aaccaaaacc aatcgaatca
    20881 gaagacttgg attgtggaaa accaatcgct acaaccatca cagaagttga ccctgacgat
    20941 ccagaatggg tagaaaaagt tgcagcattc actggtgctg tttctggtga tacctatgtc
    21001 aaattggacc acggtatcct aacagttaac caaattgata tgttcttgaa agctatgcca
    21061 tttgaattga catacgctga cgacaacaac caattcctct actacaacaa cgctcaccaa
    21121 gatccagaca ctatgttcgc taaacgtgta ccacctcaat caggtagccg tatgtcgact
    21181 gttcatggtt ctcttccacc agcacgtatg aagaacgtag agtgggttat cggaacactt
    21241 cgcaacggaa accaagaata cgtccgtacg atcgttccag gttctcctgc aggtgttatc
    21301 aacactcaca actaccaagc aatgtactat cctgatggat catacgctgg tatcaatgaa
    21361 atcgtcttta acttccaacc atggcttgac tggtacctaa aagagactgg tcaacgtttg
    21421 gtgggtggta gcggaccatt tgctcctgct gccggaggtc atggagacgc cgatgctact
    21481 tctagcgctt ctgattcagg ggatgctgga ggccacggtg gtgacgcaga cgctacagcc
    21541 ggtgcaagtt actaatgata gtttttagga actattatgg ttcctttttt agcgaaaaaa
    21601 gactagcaaa cacaaactgc tagtctcttt gttatcgttt catctatctt tgttaaaaga
    21661 aagaatacat atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgta
    21721 cttaagtacg gcaaggtgaa gctgacgtgg tttgaatttg attttcgaag agtattatta
    21781 tgaatttgga tcatcaagac tacggccatg taatcctttt tcacgttgta cttggcgtag
    21841 tttttctggt gtaacatcgt ttccttcttc gtccacaatt ttgattcctt caatgtggtg
    21901 acgaacagcg cggcgataac cttcgatgta ctcctcacgt agtttggctt gttccacttt
    21961 ttcttctggt gttaagcctt ctgttttttt ctttttagca agctcattga tacgagcaat
    22021 ttttttcgga tccatttctt ctcctttgta ataagataaa gtccgtttat caaattttat
    22081 ttagtgttaa tttggttaaa acaagcaaac ttagctgctt tcttggttag ttgtgacaag
    22141 attgccaaac gattttgacg gacagcctga tcttcagcca ttaccatagt attttcaaag
    22201 aaagcatcaa tgactgggct aagcgcaaaa agttgtttca attgctgact tgcaggtcct
    22261 gataaaatga gtgtttctac tgcttctgcc aaagcttttt cttggtcatt ctcaaatagt
    22321 gctgaatcaa ccgtagcaac cccttctgcc ttctcggcca ggttaaaggc acgagaaagt
    22381 gattcaacag atggtttaaa atcttcttcc ttgcttactt ctacgagagc acttgctgct
    22441 tccaacatat ctgccacaac aaagtttgaa cctgcaagaa ctgcttcctt gatatctttt
    22501 ggagtagagc ccatcatctt atcaacacga gccttgataa agtccataac ctctgcttta
    22561 ttttcataag tcaaactgtc aaatttcaat gcataaaggc tatcaatcag ctcatccata
    22621 gcaatgtgcc aaccaaaggc atccaagata cgaaccacac cttgagttgc acgacgaagg
    22681 gcataagggt cattagaacc tgatggaatc aatcctactg agaagaaact caaaatcgta
    22741 tccaatttgt ctgcaatggc tagaactgcg ccgaccttgc tctctggaag ttctccttca
    22801 gctgatgtag gcatgtagtg ttcacgaata gcagctgcca ccgctggagt ttcaccagca
    22861 agaagggtgt atttttcacc cataattcct tggagttcgt caaattcacc aaccatacct
    22921 gtcaacaagt caaacttgta aatggctgct gcacgagcaa ggtcaactgt ttcatccact
    22981 gacaaactag ctttttctgc caaaagtaca gtgatttgac ccgtacgaat catgtgttca
    23041 cgaagggaac caatcttctc atggaaggtg acattgttta atttttcaac aagatctgaa
    23101 atcaccaatt tttggtcttc acgccagaag aattctccgt cttccaagcg ggctaccaag
    23161 actttttcat ttcctttgat gacatttttc aaacgctctg cgtttccgtt acgaacagaa
    23221 atgaagtttg gcaagagttt tccatcttga tcacgaacaa caaagtaacg ctggtgttcc
    23281 ttcatagaag tcaccaagac ttcttctgga acttcaaggt atttagcatc aaaacttccc
    23341 atgaaggcag ttgggtattc aaccaaattc aagacttcat tcagcaaatc cgcatcaatt
    23401 tcgatacgta caccatgttt tgcctcaatt tccttgattt ggtcaacaat catttgctca
    23461 cgttcacatg gatctgcgat tacaaactgc ttacgaaggt cttcttcata gctcaatgct
    23521 gactgaatct tggtttcttg tcccaaaaaa cgatggccac gactcacacg acttcccttg
    23581 atatcaagga aatccaagtc aaactcttgc tcatccaaga gaacagttaa agtgtgaaca
    23641 gggcggatgt attcaaagct atttcccgcc cagtgcatgc tgacagggaa agtcagtgac
    23701 ttcaagacat ccacaatgcc tggaacaatg gcttcaactg cttgaccaat ttcttcctta
    23761 gtgacataga catattcttc acccttgatt tcacggaatt cgatatcttc aacagtcaaa
    23821 cctttcccac ggacaaatcc ttgagctgct ttggtgaagt ttccatcact atctaaggca
    23881 attttctttg ctggaccctt gaaatcttct gttaaatcag actgtttgtc tgcaagacca
    23941 gttacacgaa cagccaaacg acgtggtgtt gagaaagttt gaatggcttc aaaagacagg
    24001 cgttttccct tgaggaaggc tgccattttt tcgcctagtt gtttttcact tggcgtaaca
    24061 acataggctg gtaattcttc aagaccgagt tctactaata agttttttgt catgtttttt
    24121 cctttacatt ttcttcaatc ttttttgata tgcaccttag gtactgggga cgtcgctaac
    24181 taactctgtg agtctgtatg gaaatccaat gctaaattta tcaagatttc caacaatctc
    24241 atcaaagtgg atttagctga ctgatttttg aacctaaggt ttcaaaaatc cccacataac
    24301 agtacagcgg tgcatatccc tctagcaagt cttcgacttg cctctaacga ttttagagta
    24361 cagtattact cactattctg cgtcttctgc taaaagttta actcgtgttt cttcatccaa
    24421 aagtgggtaa cctaggcgtt tgcgttctgc aacaaaggtt ttggctacaa cacgggccaa
    24481 gttacggata cgagcgatat agcctgcacg ctctgttaca gatacggcac cacgcgcgtc
    24541 aagcagatta aaggtatgtg aacatttgag aacatagtca taggcagggt gtaccaagcc
    24601 ttcttctaat gcacgaccag cttctttttc aaacttatca aaattttcaa gcaacatttc
    24661 ttggtccgaa atttcaaatg aatattttga gtgctcatac tcaggctgga taaagatttc
    24721 tccgtatttt acaccatcag cccactcgat atcatagaca gagtctactt cttgaatgta
    24781 agaagccaag cgctccaaac cataggtaac ttccgcagtc acagggctag ttgccaatcc
    24841 accgacttgt tggaaataag tgaactgagt gatttccatt ccgtcaagcc aaacttccca
    24901 accaagacca gctgaaccag ttgatgggtt ttcccagttg tcctcaacaa aacgaatatc
    24961 gtgctccaaa ggattgattc ccaatttttc caaagactca aggtaaagtt cttggatatt
    25021 tgatggagaa ggcttcatga ccacctggaa ttggtggtgt tggtagagac ggttagggtt
    25081 ttccccataa cgaccgtcag caggacgacg tgatggctct acataagctg cattccatgg
    25141 ctcaggtccg atagcacgaa ggaaagtgta aggactcatt gttcccgcac ctttttcatt
    25201 atcataagcc tgcataagca tacaaccttg gtcattccaa aattgttgca aagtaaggat
    25261 gatttcctgg aaagtcaatt tcttagacat tgtttactcc tttagttttt atatttcttt
    25321 ttcaattaat ttctgctgaa caaaccaatc taacaaggga aaagtttttt ctgtatttgc
    25381 ccataaatga taagaatcta agaccgcttg tacactacct aaattttcta ccaacttatc
    25441 tactgtcaaa aagctgcgcc agtattcata aaaaatactt gcatagttct tatcataagt
    25501 cgaagaacca aaatcattca atgaatgcca accatacttc ttctgaaaaa gttctacgag
    25561 agattgatta caaatttttt ccgcttgaaa ttcctcttct gtcaagaaat acttgcgact
    25621 aatatattca accatcccct cttcgaacca gatataagag tcataaccat caaaatcatc
    25681 taaaaaatgc tccgaccaat gagctaactc atgtcctaca atctgtaaga ggaaattttc
    25741 agataaagaa tgatagtgac tttctattgc ttgagtttgc tcagaacact cgtagtccat
    25801 caattgatac aaatacaact ctttccaaac agctaaatca ggcgccataa ccattcgtct
    25861 attatttgta taggctggaa cagcgctttc cctaataatc tgtgtagcag cattaaaatc
    25921 tgaccaaata acggcttgcg gtaaatcaca gactgcaagt tcattcttta aaaaagattg
    25981 ataatctttc aacttttcag tatttcttac tacaaaatca cgaaatgtag ctagttgact
    26041 atcgtctttt acaagataaa ggttgtccac ggctctcctt tctttcacac aatagactga
    26101 ataagcaaaa gaaccacatc tgacaatcct aggttgattt aacctagggg cgtcagaaac
    26161 gcggttccac cctaatttat tacttcattg tttttgaaaa tactacagaa agcgccagct
    26221 cattattttg ccctacttgg ctcccactgt cccaagctcg cttgaagaac gccataagac
    26281 tttctttcct gctgactatt atatcaaaaa ttagaataat gtacaattct ttttatgatt
    26341 ttctagaaat ccacatcatc aactcgtgga gcccccgact gaacagcagt cgtttttaag
    26401 gtttctctct cctcatgact cagttcaatt ccaaaacaat caagattagc ttgaatacga
    26461 gaggttgtga cagattttgg aagtggtaaa aatccttctg ccaagctcca ggccaaggct
    26521 atctgagcaa ccgattttcc gtgatttgct gcgatttctt ggacttgctt gctatcaaac
    26581 agttctcctt gtccaaaagg cccccaagct tccaataaaa ttcccttttc acgacagtaa
    26641 gctacgactt cctcttgata cacacctggc gccaagcgaa cttgattgac cgcaggaacg
    26701 atagttgcag tttcaagcaa ggcatccaaa tgatggggaa gaaaattgct aacgccgata
    26761 gcacggattt tcccttcttg atagaggtct tccatcgctc tccaaacttc cgcattgcga
    26821 gttttccatg cgtcattttc tctgagcggt tttgggttcg gccaatgaat caaatacaaa
    26881 tccaaataat ccaagcccag tttttctata gatttttcca aagcttgacg agtttgctca
    26941 taggtttgct gactattcca aagcttggta gttacgaaca tttcttcacg tggaactccg
    27001 ctatctttga ttgcttgacc aacactttct tcattctgat aaatcgccgc cgtatcaata
    27061 tgacgataac cagccttcaa ggattctaac actgcacgat aggcttcttc tccatcctta
    27121 gccttaaaag ttccaaatcc caatactgga atttctactc cattatttaa ttgataggta
    27181 ttcatatgct tctccttcta tatttattca tcaattatag cataaaacat tttttaagta
    27241 gtactatctt ctgtaaaaaa taagtctgtc aaatatctaa cagacctatt ttttcttgct
    27301 tattcatcac gactatagta cagctcatga gcttttaggc gagtattgag catctctggt
    27361 atggtcacaa aggtataacc ttgatttttc aaatactcaa tgacccttgg caaggcattg
    27421 actgtcggac tgtgaatatc atgcatcaaa acgatagagc cgttagctac ttgatgctga
    27481 atttctgtca aaatagatgc ttcattttta ctcttccagt ccagactatc cacatcccac
    27541 atgataaagc tcaaatccaa gctattgcga atatcatctg taatagcacc ataaggtgga
    27601 cgcatgagtt tagaactaga acccagcact ttagttagca catcctcagt atcagtaatc
    27661 tgctttttag cttcatcaag agagagttgc gagagaatcg gatggctcca actatggttt
    27721 ccaacaacat gaccttcaga ttttatcctc ttcaccaagt cctcattccc agaaacattt
    27781 ttcccaagca caaagaaagt cgctttaata tcatatttag ctagggtctc taatacctgc
    27841 ggggtcgttg ctggatttgg accatcatca aaggttagag cgacaacttt ttgatatttc
    27901 ttatcaaagt aagattggta caaggccgca tctttttcga gtaagtacga agattggata
    27961 acatcaaaga aagcagatac tggcaaggct atctcttcta aattttcaac cacaggactt
    28021 ggataaagga taatctgact atccttgtaa tcaaaattcc atgcagacaa gtcttggtca
    28081 gagaagtttt ttacaatctg ctcactctgg tcttgctcta tttttttatc ctctatgaag
    28141 gaggtcaact cttttatcag ctgttcctta gccttactag catctgaaaa cagttggtca
    28201 agtgtaaaag gttgcccatc ttctgttaag tgaacctttc ctagactggt cttttcagtc
    28261 tcttcaacct taaaagcaac taaatcatag atttgtttgg tcacattacg attaacgact
    28321 ccctttaaac ctgactcttc ttgctctgta tagtagaaaa caagattgtc cttactttct
    28381 agcttgtcct taacatcttg atttatcaac tccctaacag aggaaatcac tttctcccct
    28441 tggagaggat aataggcaat cacttcggct tgcccctgac gaaaatgctc cttctgattt
    28501 ccctcactca attgatcatc tttctctttt ttgagcgatt caatcttttg ctcaaaactt
    28561 ttttgctggt agatcttaaa agcaataaat ccgcctaata ggcaaataga aattgctaaa
    28621 ataccaatca aagccaataa tacatgtctc gttttcccgt gtctgccacg tcctagtcta
    28681 cttttattca tatttatatc ataacaaatt tgagcaataa tttcaatgat acaatttggc
    28741 aatccacaat tcctctagca agccaaagca tcttacatca actccaacat ccgtatctgt
    28801 agagattatg tagttttcta tctatggaat ttctagtaga ttgaagctag aatagtagac
    28861 ctatacttct aaaacattgt tagaaatcga tttgattgtc ctgatcgatt tgtcctgttc
    28921 tcatttcatt ttactataaa actatgaaaa gctaggtcat ctgagaagta attactatcg
    28981 acaattgcga tagcgtatac tggtgcgatt tgtttgtagc tctggtgggt atcaccttca
    29041 cgttggcgaa ttttttctag gttttgatta acctgactgc acagataagc ccataggcga
    29101 ttgatgaaaa aattctgatg atgaacctga atttcgatga taacttggat tccattatct
    29161 aactccgcca agacgtctat actagtatag aaatcctgcg ctgagtaggg catggaaggc
    29221 aagacgtgaa tgttacttcc ctccaaaatc gtcacatttt ttgctggcaa gtccagcata
    29281 tcgcggataa attgacaaat cattagttgg gctgataccc ggatgtctga gaatcctcct
    29341 tttcctcctt ctattatagc acatttttaa atctaggttt gctagattct atgcttctat
    29401 ctatttattc ggaaaagaaa gctaatagct tatttgaaat tttctcctct ctgttggata
    29461 gtcccgtcta tctatgttat aatgaaagaa ggatttaaaa tcggaaaagg agtatttatg
    29521 ctaaaattag gtgtcatcgg aactggcgct atcagccatc atttcataga agcagcccat
    29581 accagtggag aataccaact ggtcgcaatc tattctagaa aactagaaac tgcagcaacc
    29641 tttgcttctc gctatcagaa tatccaacta ttcgatcaat tagaagtctt cttcaaatct
    29701 tcttttgatt tagtctatat cgccagtcca aactccttac attttgctca ggcaaaagct
    29761 gccttgtctg ctggtaaaca tgttattctc gaaaagccag ctgtcactca accacaagaa
    29821 tggtttgatt tgattcaaac agctgaaaaa aataactgtt ttatctttga agcagctcgt
    29881 aattaccacg agaaagcctt tactactatc aaaaactttt tagcagataa gcaagtgtta
    29941 ggagcagatt tcaactatgc caaatactct tccaagatgc ctgacttgtt gactggacaa
    30001 acaccaaatg tcttttcaaa ccgttttgct ggtggagccc tcatggattt ggggatttat
    30061 cccctctatg ctgctgttcg tctttttgga aaagccaatg acgcgactta ccatgctcaa
    30121 cagcttgaca atagcattga cctaaatgga gatggtatcc tcttctaccc agactatcaa
    30181 gttcacatca aggctggaaa aaacatcact tccaatcttc cttgcgagat ttatacaaca
    30241 gatggaacct tgactctcaa cacgattgag catattcgct cagctatttt taccgaccac
    30301 caaggaaatc aagtccagct tcctatccaa caggctcctc atacgatgac tgaggaagtc
    30361 gctgcatttg cacacatgat ccagcaacca gatctgaatc tctaccaaac ttggctgtat
    30421 gatgcaggct ctgttcatga gctactatat accatgcgcc agactgctgg cattagattt
    30481 gaggcagaaa aatgaaaacc aaactaccga ctgaatggca agaactgagt gaccaactcg
    30541 gtttccaaga attcaccccc attcaaactc aactatttga gccccttctt gctggagaaa
    30601 acctcctagg agtgagccaa accggaactg gtaagaccct agcgtatctc ctaccaagtc
    30661 ttctcagact acaaaagaaa aaagcccaac aactcttgat tctagcacca aacacggaac
    30721 ttgctggaca gatttttgat gtatgtaaaa cgtgggcaga agctatcgga ttgactgctc
    30781 aactcttcct ttcaggttca agtcagaaac gacaaattga acgccttaaa aaggggccag
    30841 aaattctgat tggaactcct ggccgtatct ttgaactcat taaacttaaa aaaatcaaga
    30901 tgatgaatgt ggaaaccatc atcctggatg aatttgacca attactagat gattctcaga
    30961 ttcactttgt agaaaaaatc actcactacg cacctcgtga ccaccaactg gtctacatga
    31021 gtgccacgac caagtttgac caagaaaaga ttgtgccgaa tacacgcact attaatctct
    31081 ctgaccaaaa attggacaat atccagcatt tctacatgca ggtagaccaa cgtcatcgag
    31141 tggatatgct acgaaaactg gctcatgtag aggatttccg tggtctagtc ttcttcaata
    31201 gcctgtcaga ccttgggaat gccgaggaaa aactacagta tcgtgatata ttgactgttt
    31261 ccctcgctag tgatgttaat gttaaattta gaaaaatcat cttagaaaag tttaaagata
    31321 accaactaac cctgcttctt gcaactgacc ttttggctcg tggaattgat atcgatagcc
    31381 tagaatgtgt cgtaaacttt gatattccta gagatagcga aacctacaca caccgtgctg
    31441 gccgtactgg ccgtatgggc aaggaaggat atgttattac tctcgtcact catccagaag
    31501 aaatcaaaaa acttaaaaag tttgcaagta tccgagaaat tgtcctaaaa aaccaagaac
    31561 tctatatcaa ataaagcccc attgctctat acacatccag tgtatggagc ttttttataa
    31621 gcaattcaat tttatactca tctgctttca aaaagcattc tattccatct ccgattaacg
    31681 atggacttta tcacctcctt ctccagtcct tgtatgacat cttggagttg attcatgaca
    31741 tcttccaaag ttcgaaaggc tttattctta aatccacgtt tacgaatctc tttccacact
    31801 tgttcaatgg gttcatctct ggtgtgtatg gaggaataaa ggtaaaatca atattagtcg
    31861 gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt aaaaaataat
    31921 catctggata agcttgtgaa agctcttcta aaaagacgtt catccactca gtattacatc
    31981 caccagctat taagaaaaat gattcgcctg tatggggcat caacagctcc ataacaatag
    32041 cgaaattctc gtatatagtg actatggaca tgtggaccta ctcctattgg agaccaacaa
    32101 gatcccagtt tactgattct accgaaacca gcctcatctt ggtacatcaa gcgaacctta
    32161 tgaaaaggtc tactggtttt aaagcgcttt cttgtcttct tgaattgaga ttttattttt
    32221 agacgcgaca atggtttgag cgtctgcttt cctaggatgt tctggacgtg gcataatatt
    32281 tcgccaacca tggtgcttca acagttgata gaaggcatca cgtgtgtagg aacgatataa
    32341 gcctgaaata aggtattgta acaaattctc ctgcctctgt agccttcaaa tggcgggcaa
    32401 gaaaggcttt ctcttcctca actgtcatat atgcatggtt acgaccacca cgtgtttctt
    32461 gaaggagaga gtcgagtccg aactcctcat atttttttac atttcgccaa atcgttgttt
    32521 gattacagtc taaaagatct ataatctctt tataagattt gcccatcaga cgaaatagaa
    32581 cgatttgaag gcgtttatga tatttagctg tacgaaagtc ttttaaaagt gttttgatgg
    32641 tttggatttc ttctttagtt gatttcatat tactattgta taatgctttt tgatttgagt
    32701 ctagtattat actcttcgaa aatctcttca aaccgcgtca gcttcgcctt gtcgtataga
    32761 atgttactga cttcgtcagt tctatctaca acctaaaaac agtgttttga gcagtctgca
    32821 gctaactttc tataacaaaa tgaaataaga acaagacaaa tcaatcaaga caatcaaatc
    32881 gatttctaac aatgttttag aagtagatgt gtactattcc agtttcaata tattatagtt
    32941 tgctctttga ttttcattga gtattagttc aatatct
//
LOCUS       DAVLFB010000013        32917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLFB010000013 DAVLFB010000000
VERSION     DAVLFB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.013"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            147..935
                     /gene="codY"
                     /locus_tag="VXD58_000536"
     CDS             147..935
                     /gene="codY"
                     /locus_tag="VXD58_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV0675282.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            935..1510
                     /locus_tag="VXD58_000537"
     CDS             935..1510
                     /locus_tag="VXD58_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0675283.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1592..1677
                     /locus_tag="VXD58_000538"
     tRNA            1592..1677
                     /locus_tag="VXD58_000538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Leu,seq:aag)
     gene            1960..3090
                     /gene="ugpC"
                     /locus_tag="VXD58_000539"
     CDS             1960..3090
                     /gene="ugpC"
                     /locus_tag="VXD58_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV0675284.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            3591..4349
                     /locus_tag="VXD58_000540"
     CDS             3591..4349
                     /locus_tag="VXD58_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0675285.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4437..4949
                     /locus_tag="VXD58_000541"
     CDS             4437..4949
                     /locus_tag="VXD58_000541"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV0675286.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5131..6075
                     /locus_tag="VXD58_000542"
     CDS             5131..6075
                     /locus_tag="VXD58_000542"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV0675287.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            6084..6761
                     /locus_tag="VXD58_000543"
     CDS             6084..6761
                     /locus_tag="VXD58_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV0675288.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6859..7617
                     /locus_tag="VXD58_000544"
     CDS             6859..7617
                     /locus_tag="VXD58_000544"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV0675289.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7655..9160
                     /gene="lytC"
                     /locus_tag="VXD58_000545"
     CDS             7655..9160
                     /gene="lytC"
                     /locus_tag="VXD58_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV0675290.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9658..10176
                     /locus_tag="VXD58_000546"
     CDS             9658..10176
                     /locus_tag="VXD58_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV0675291.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            10306..10812
                     /locus_tag="VXD58_000547"
     CDS             10306..10812
                     /locus_tag="VXD58_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV0675292.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEKKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10812..10982
                     /locus_tag="VXD58_000548"
     CDS             10812..10982
                     /locus_tag="VXD58_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675293.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            11014..12246
                     /gene="clpX"
                     /locus_tag="VXD58_000549"
     CDS             11014..12246
                     /gene="clpX"
                     /locus_tag="VXD58_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV0675294.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIDEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12255..12842
                     /locus_tag="VXD58_000550"
     CDS             12255..12842
                     /locus_tag="VXD58_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV0675295.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHAPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12853..13233
                     /locus_tag="VXD58_000551"
     CDS             12853..13233
                     /locus_tag="VXD58_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV0675296.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13285..14175
                     /gene="rapZ"
                     /locus_tag="VXD58_000552"
     CDS             13285..14175
                     /gene="rapZ"
                     /locus_tag="VXD58_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV0675297.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14172..15149
                     /locus_tag="VXD58_000553"
     CDS             14172..15149
                     /locus_tag="VXD58_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV0675298.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15146..16057
                     /gene="whiA"
                     /locus_tag="VXD58_000554"
     CDS             15146..16057
                     /gene="whiA"
                     /locus_tag="VXD58_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV0675299.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16091..17059)
                     /locus_tag="VXD58_000555"
     CDS             complement(16091..17059)
                     /locus_tag="VXD58_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV0675300.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEDYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQTLQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17195..18052
                     /locus_tag="VXD58_000556"
     CDS             17195..18052
                     /locus_tag="VXD58_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV0675301.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            18039..18818
                     /locus_tag="VXD58_000557"
     CDS             18039..18818
                     /locus_tag="VXD58_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV0675302.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18842..20194
                     /locus_tag="VXD58_000558"
     CDS             18842..20194
                     /locus_tag="VXD58_000558"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV0675303.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20268..20642
                     /locus_tag="VXD58_000559"
     CDS             20268..20642
                     /locus_tag="VXD58_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV0675304.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20644..21492
                     /locus_tag="VXD58_000560"
     CDS             20644..21492
                     /locus_tag="VXD58_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0675305.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21769..22536
                     /locus_tag="VXD58_000561"
     CDS             21769..22536
                     /locus_tag="VXD58_000561"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV0675306.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22548..23732
                     /locus_tag="VXD58_000562"
     CDS             22548..23732
                     /locus_tag="VXD58_000562"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV0675307.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDLDLLLQAENLRQAQGKEVNPQVIKETYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23729..25600
                     /locus_tag="VXD58_000563"
     CDS             23729..25600
                     /locus_tag="VXD58_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0675308.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAQDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25743..26927)
                     /gene="mntE"
                     /locus_tag="VXD58_000564"
     CDS             complement(25743..26927)
                     /gene="mntE"
                     /locus_tag="VXD58_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV0675309.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27231..29927
                     /locus_tag="VXD58_000565"
     CDS             27231..29927
                     /locus_tag="VXD58_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV0675310.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29940..30731
                     /gene="yghU"
                     /locus_tag="VXD58_000566"
     CDS             29940..30731
                     /gene="yghU"
                     /locus_tag="VXD58_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV0675311.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLRTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30847..31257
                     /locus_tag="VXD58_000567"
     CDS             30847..31257
                     /locus_tag="VXD58_000567"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV0675312.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31269..32864
                     /locus_tag="VXD58_000568"
     CDS             31269..32864
                     /locus_tag="VXD58_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675313.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGRTFEGQVGKDSYLKTFKVQ"
BASE COUNT         9691 a         6125 c         7590 g         9511 t
ORIGIN      
        1 caatagaaaa ggaacaattt aattaaaaat attctggttt ttaattaaat tgtaagtcaa
       61 tgcaagtcaa taaggaattt tcagataatt attgaaaaag agacgttttt atgttataat
      121 gatagcgatt aaattcgagg tgaaaaatgg cacatttatt agaaaaaact agaaaaatta
      181 catcaatttt gaaacgctca gaggagcagt tgcaggatga gctcccttac aacgctatta
      241 cgcgtcagtt agcggatatt attcattgca atgcctgcat tatcaatagt aagggacgtc
      301 tgcttggcta ttttatgcgt tataaaacaa atacagatcg cgtagagcaa ttcttccaaa
      361 ctaagatttt cccagatgac tacgttcaag gggctaatat gatttacgaa acagaagcaa
      421 acttgcctgt tgagcatgat atgagtattt tccctgttga gagtagagat gattttccag
      481 atggcttgac gactattgca ccgattcatg tatcggggat tcgccttggt tctttgatta
      541 tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt tcttgttgag attgccagta
      601 ccgttgttgg gattcagctt cttaacttcc aacgtgaaga agatgagaaa aatattcgtc
      661 gtcgtactgc tgtcaccatg gcggtcaata ccctttctta ctccgaactc cgtgctgttt
      721 cagcaatttt aggggaatta aatggaaatg aagggaagtt gactgcgtca gtgattgcag
      781 atcgtatcgg aatcactcgc tctgtgattg tcaatgctct tcgtaaactt gagtctgcgg
      841 ggattattga aagtcgctca cttggaatga agggaaccta tcttaaggtc ttgatttcag
      901 atatttttga agaagtgaag aaaagagatt actaatgaca aaggctttaa tttcgattga
      961 ttatacagaa gattttgttg ctgatagtgg gaagctgaca gcaggtgctc cagctcaggc
     1021 gatttcggat gccatcagca aggtaactcg attagctttt gaacgaggag attatatctt
     1081 ctttactatt gatgctcatg aagaaaacga ttgtttccat ccggaaagta agttatttcc
     1141 tcctcataat ctgattggga cgagtggacg gaatttatat ggagatttgg ggatctttta
     1201 tcaagagcat ggttcagaca gtcgtgtctt ttggatggat aaacgccatt actcagcttt
     1261 ttcagggact gacctagata ttcgtttgag agagcgtaga gtgtctaccg ttatcttaac
     1321 aggtgtcttg acggatatct gtgtcctaca tacagctata gatgcctata atctaggata
     1381 tgacatcgag attgttaaac cagctgttgc ttccatctgg cctgaaaatc atcaatttgc
     1441 cctaggtcat ttcaaaaata cactcggagc taagttagta gatgaaaatc taaatgaact
     1501 ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa aaaagtgttg acaagcatcc
     1561 caaaagttga tatactagta aagtaatcga tgcggggatg gcggaattgg cagacgcgca
     1621 ggactaagga tcctgtgacc gctttaggtc gtgagggttc aagtccctct ctccgcatag
     1681 gaaaagagtt agctaaagct agctcttttg tatttctcta aaaaattaga atccagtcaa
     1741 atgtagtgga gaatcagcga aaatatggga aaactgatcc ttgtttctgc ttttgtcata
     1801 gaagaataat aaagattatc ttcttcaaat tctccgatat tctctaaagt tttgtgcaag
     1861 ttgcacagaa cttttttatt tttttggtca tcttgccata gaaatataaa gcgttttcat
     1921 ctataatata attatcaaaa gacaaaagga gttcacctca tggtagaatt gaatcttaaa
     1981 aatatttaca aaaaatatcc aaacagcgaa cactattcag ttgaagattt caacttgaac
     2041 atcaaagata aagaatttat cgttttcgta ggaccttcag gatgtggtaa atcaactaca
     2101 ctccgtatga ttgctggtct tgaagacatc acagaaggta ctgcatctat cgatggcgtg
     2161 gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca tggtattcca aaactacgct
     2221 ctttacccac acatgactgt ttatgacaac atggctttcg gtttgaaatt gcgtaaatac
     2281 agcaaagaag acattaacaa acgtgttcaa gaagcagctg aaatacttgg attgaaagaa
     2341 ttcttggaac gtaaaccggc tgacctttca ggtggtcaac gtcaacgtgt tgccatgggg
     2401 cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg aacctttgtc aaacttggat
     2461 gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa ttcaccgtcg tatcggagct
     2521 acaactatct atgtaactca cgaccaaaca gaagcgatga cacttgcaga ccgtatcgtt
     2581 attatgtcag ctactaagaa ccctgctggt acaggtacta tcggacgtgt agaacaaatc
     2641 ggtactcctc aagaagttta caaaaatcca gttaacaaat tcgttgcagg attcatcgga
     2701 agcccagcta tgaacttcat caacgtgaaa ttggttggta gcgaaattgt ttctgacggt
     2761 ttccgtttga aagtgccaga aggagcattg aaagttcttc gtgaaaaagg ctacgaagga
     2821 aaagaattga tctttggtat ccgtccagaa gacgtgaatg cagaacctgc tttccttgaa
     2881 acattcccag actgtgttgt aaaagcgact atctctgtat cagaactgct tggttcagaa
     2941 tctcaccttt actgccaagt tggtaaagac gagtttgttg caaaagttga tgctcgtgac
     3001 tacttgcaaa caggtgcaac agttgagctt ggatttgact tgaacaaagc acacttcttc
     3061 gatgtagaaa ctgaaaaaac aatctactaa gaataaataa aattcaaagc actacaagaa
     3121 aagatatctc tttatcaatt gtagtggaga gatatcagtt aatctaggga gagaaacaaa
     3181 atgcttctct cctttttgct agggaagtca tattatacat ctatattgtg atgctcttta
     3241 tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtacgt atgattactg
     3301 acttcgtcag ttttatctgc aacctcaaag ctgtactttg agtagcttac ggctagtttc
     3361 ctagtttgct ctttgatttc cattgagtat tatttgtggg taccatctac aagtgaagct
     3421 atatgcgtaa actacgtgag caattgaatt cgaactagag aggtaataat aaatttatgc
     3481 tatagttatg gtgacttgta tgcttttgat tctagtttat caaataatag attagaattg
     3541 tcagataata tcattttgtg ttataatgaa gaaaaaacag aggtgttcaa atgtcagaag
     3601 caggtcataa gtttttagca aaattgggga aaaaacgctt acgtccaggt ggaaagcgtg
     3661 ccacagattg gttaattgca gaaggaggat tttcaaaaga aaagagaata ctagaggttg
     3721 cgtgtaatag gggaactaca gcaattgagt tggcacagcg ttttggttgc aagataactg
     3781 ctgttgatat ggatgctcaa gctttagaag tggctaaaaa atctgctgga acggcaggtg
     3841 ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa acttccttat caagatgcta
     3901 gttttgatat tgttataaat gaagctatgc tgactatgca agccgatcaa gctaagaaaa
     3961 aatgtgtaat ggaatatcta agggtattaa aacctggagg tcttctcttg acacatgatg
     4021 tgcttcttaa ggaagctaaa gagtctatca gacaggaatt atcacaagca attcatgtaa
     4081 atgtaggtcc tttaactcaa gatggttggg aacaggtgat gatagaatca ggttattgtg
     4141 atgtgaaagc attgactggt gaaatgacat taatgaaatt atcgggtatg atttatgacg
     4201 aaggtttgct aggaactttg aaaatttgtg taaatgcttg taaaaaggag aatagaaagc
     4261 agtttttaac tatgtataaa atgtttgcta agaataaaca gaaattgggc tttattgcga
     4321 tggctagtta taaatcgtca aaacgttaga taattattga agttaacttt tccttttttc
     4381 tttcttaaaa aatatgctat aatagagagt aaaaaacttt gaaagaaaga aaaaagatga
     4441 atttaaaaga ttacattgca acaattgaaa attatccaaa ggaaggcatt accttccgtg
     4501 atattagtcc tttgatggct gatggaaatg cttatagcta cgctgttcgt gaaatcgttc
     4561 agtatgctac tgacaagaaa gtcgacatga tcgtgggacc tgaagctcgt ggatttatcg
     4621 tgggttgtcc agttgccttt gagttgggaa ttggttttgc gcctgttcgt aagccaggta
     4681 aattgccacg cgaagttatt tctgctgact atgaaaaaga gtacggtgtc gataccttga
     4741 ctatgcacgc ggatgccatt aagccaggtc aacgtgttct tattgtagat gaccttttgg
     4801 cgacaggtgg aactgttaag gcaactatcg agatgattga aaaacttggt ggtgttatgg
     4861 caggttgtgc cttccttgtt gaattggatg aattgaacgg ccgtgaaaaa atcggtgact
     4921 acgactacaa agttcttatg cattattaat gaaaacagtc cctagggctg ttttctctac
     4981 actaggatat aaaaatagac tataactagt tagagaaaaa ctataattga aaactatatc
     5041 ttcttgcagt ataataaaag gactaagtgt ttgagatttg tcttcaaaca catgcaatta
     5101 ttcctgagag agtacagtta ggagagggtt atgccgattc gaattgataa aaaattgcca
     5161 gctgttgaga ttttacggac agagaatatc tttgtcatgg atgatcaacg tgctgcccac
     5221 caagatatcc gtcctttgaa gattttaatt ttaaatctca tgccacagaa aatggtcaca
     5281 gagacccagt tgttgcgcca cttggctaat acacccctac aactggatat tgattttctc
     5341 tatatggaga gccaccgttc taaaacaact cgttcagagc acatggagac cttctataaa
     5401 acttttcctg aagtcaagga tgagtatttt gatgggatga tcatcacggg tgctccagtt
     5461 gagcatttac catttgagga agtggactat tgggaggaat ttagacagat gcttgagtgg
     5521 tctaagactc atgtctattc gacccttcat atctgttggg gagctcaggc tgggctttat
     5581 ctgcgctatg gtgtagaaaa ataccagatg gacagtaagc tatcaggtat ttatcctcag
     5641 gacaccctaa aagagggtca ccttctattt agaggctttg atgatagcta tgtatcccct
     5701 cattcacggc acacggagat ttctaaggaa gaggtcttaa acaagactaa tctcgagatt
     5761 ttatcagaag gacctcaggt tggggtttct attttggcca gtcgtgattt acgagaaatt
     5821 tatagttttg gtcatttgga gtatgaccgt gataccttgg caaaagagta ttttcgagat
     5881 cgtgatgcag gtttcgaccc acatattcca gaaaattact ttaaagatga tgatgttaat
     5941 caggtacctt gtctttgttg gtcttcatct gcagccctct ttttcagtaa ttgggtaaac
     6001 catgcggtct atcaggagac gccttttgac tggagaaaga tagaagatga tgcatctgca
     6061 tatgggtatt tataagagga attatgacat atttagacgc ttttaaatca ggtaccttgg
     6121 ttttaccgag tgccctgctc ttgcatttta aggaactctt tccttctagc gacgattttc
     6181 tggtttggca atttttctat ttgcaaaata cgacaggctt agaagaaatg tcgccaagcc
     6241 agattgctga aaggattggc aaggaaattt cggatgtcaa ccagtccatt tctaatctga
     6301 cggaaagggg actgctccag tatcgtacta tcgaattaaa tggcgaaatt gaattgctct
     6361 ttgatgctag tttggccttg gaacgtttgg atgacctgtt tggagcagtt cattcaagtt
     6421 cagaccagct aacaccacaa aaccagctca aggatttggt ggaaaccttc cagcaggagt
     6481 tgggacgatt gttgacgcct tttgagattg aggatttgac caagacacta aaggaagatg
     6541 gaaccagtgc tgacttgatt aaggaggctc ttcgtgaagc tgttttgaat ggaaaaccaa
     6601 actggaagta cattcaggcg attttgagaa actggcgcca tgaaggaatc aagagtgtgg
     6661 ctcaaattga ggccaagaga gcagaaagag aagcaagcaa tcctcagttg acacaggtat
     6721 ctgcagattt cagaaatgcc atggatctct ggaaggatta atccatgcaa gtaggcttga
     6781 aatccgagta agatttgcaa gctgtgtata attgtgatag aataaataga aaataaattg
     6841 aaaaaagagg tatgtgaaat gtcacgtaaa ccatttatcg ctggtaactg gaaaatgaac
     6901 aaaaatccag aagaagctaa agcattcgtt gaagcagttg catcaaaact tccttcatca
     6961 gatcttgttg aagcaggtat cgctgctcca gctcttgatt tgacaactgt tcttgctgtt
     7021 gcaaaaggct caaaccttaa agttgctgct caaaactgct actttgaaaa tgcaggtgct
     7081 ttcactggtg aaactagccc acaagttttg aaagaaatcg gtactgacta cgttgttatc
     7141 ggtcactcag aacgccgtga ctacttccat gaaactgacg aagatatcaa caaaaaagca
     7201 aaagcaatct ttgcgaacgg tatgcttcca atcatctgtt gtggtgaatc acttgaaact
     7261 tacgaagctg gtaaagctgc tgaattcgta ggtgctcaag tatctgctgc attggctgga
     7321 ttgactgctg aacaagttgc tgcctcagtt atcgcttatg agccaatctg ggctatcggt
     7381 actggtaaat cagcttcaca agacgatgca caaaaaatgt gtaaagttgt tcgtgacgtt
     7441 gtagctgctg actttggtca agaagtcgca gacaaagttc gtgttcaata cggtggttct
     7501 gttaaacctg aaaatgttgc ttcatacatg gcttgcccag acgttgacgg tgcccttgta
     7561 ggtggtgcgt cacttgaagc tgaaagcttc ttagctttgc ttgactttgt aaaataatca
     7621 gtaagtagca aaagctaggt ggaacagcat tcagatgtct gttacatttt ttataggaga
     7681 gcaaagattg aaaacaaaaa ttggattagc aagtatctgt ttactaggct tggcaactag
     7741 tcatgtcgct gcaaatgaaa ctgaagtagc aaaaacttcg caggatacaa cgacagcttc
     7801 aagtagttca gagcaaaatc agtcttctaa taaaacgcaa acgagcgcag aagtacagac
     7861 taatgctgct gcccactggg atggggatta ttatgtaaag gatgatggtt ctaaagctca
     7921 aagtgaatgg atttttgaca actactataa ggcttggttt tatattaatt cagatggtcg
     7981 ttactcgcag aatgaatggc atggaaatta ctacctgaaa tcaggtggat atatggccca
     8041 aaacgagtgg atctatgaca gtaattacaa gagttggttt tatctcaagt cagatggggc
     8101 ttatgctcat caagaatggc aattgattgg aaataagtgg tactacttca agaagtgggg
     8161 ttacatggct aaaagccaat ggcaaggaag ttatttcttg aatggtcaag gagctatgat
     8221 gcaaaatgaa tggctctatg atccagccta ttctgcttat ttttatctaa aatccgatgg
     8281 aacttatgct aaccaagagt ggcaaaaagt gggcggcaaa tggtactatt tcaagaagtg
     8341 gggctatatg gctcggaatg agtggcaagg caactactat ttgactggaa gtggtgctat
     8401 ggcgactgac gaagtgatta tggatggtgc tcgctatatc tttgcggcct ctggtgagct
     8461 caaagaaaag aaagatttga atgtcggctg ggttcacaga gatggtaaac gctatttctt
     8521 taataataga gaagaacaag tgggaaccga acatgctaag aaaatcattg atattagtga
     8581 gcacaatggt cgtatcaatg attggaaaaa ggttattgat gagaacgagg tggatggtgt
     8641 cattgttcgt ctaggttata gcggtaaaga agacaaggaa ttggcgcata acattaagga
     8701 gttaaaccgt ctgggaattc cttatggtgt ctatctctat acctatgctg aaaatgagac
     8761 cgatgctgag aatgacgcta aacagaccat tgaacttata aagaaataca atatgaacct
     8821 gtcttaccct atctattatg atgttgagaa ctgggaatat gtaaataaga gcaagagagc
     8881 tccaagtgat acagacactt gggttaaaat catcaacaag tacatggaca cgatgaagca
     8941 ggcgggttat caaaatgtgt atgtctatag ctatcgtagt ttattacaga cgcgtttaaa
     9001 acacccagat attttaaaac atgtaaactg ggtagcggct tatacgaatg ctttagaatg
     9061 ggaaaaccct tattattcag gagaaaaagg ttggcaatat acctcttctg aatacatgaa
     9121 aggaatccaa gggcgcgtag atgtcagcgt ttggtattaa gcgatgattt gaaagaggga
     9181 tgtgatagta gcaccctctt tttctttgtt ttatgatagt tcatcctcga gtaaattcaa
     9241 gatcttgctc ggaaatgaag cttatatagt agattgaata tagacaaata ccttgtgatt
     9301 ggtaaaacat tttagaaatt catttacctt tcctaatcga cttggtttca tcttatttca
     9361 atctattata gtattgggga atttcttcaa accacatcag cttggtcagt tctacctgcg
     9421 acctcaaaac ttgtgctttg gtcaagctgg gtttagtttc ctagtttgct gatggatttc
     9481 cattgactat aagcatccaa ccctcttttt gtcttctaaa gaattcttaa attatcagtc
     9541 tattgcaact tttctcatgt gggtcatttg gcttgcaatt gtctttcctt agtagtatac
     9601 tgaggtagta atcattaaga agtggttaca taaaaataat gaatgaggta aaataaaatg
     9661 gtagaattga aaaaagaagc agcaaaagat gtcgcaacat taacaaaagc agcgccagta
     9721 gcattggcaa aaacaaagga agttttgaat caagcagttg ctgatttgta tgtagctcac
     9781 gttgctttgc accaagtgca ctggtatatg catggtcgtg gtttccttgt atggcatcca
     9841 aaaatggatg agtacatgga agctcttgat ggtcatttag atgaaatcag cgaacgcttg
     9901 atcacacttg gtggaagccc attctctact ttgacagagt tccttcaaaa cagtgaaatc
     9961 gaagaagaag ctggtgaata ccgtaatgtt gaagaaagct tggaacgtgt tctagctatt
    10021 tatcgctacc tcatcactct tttccaaaaa gcgttggatg tgactgacga agaaggcgat
    10081 gatgttacaa acgatatttt tgttggtgct aaggctgaac ttgagaaaac agtttggatg
    10141 cttgccgcag aacttggaca agcacctggt ttgtaagaaa caagacaaca tagataaatc
    10201 tgcaggaaat ttcttacaga ttttttctat tttgtaagct attccaaacc agtctttttt
    10261 tgagtttttt gataaaatag tactatcagt gaaaaggatg gaagcatgac taagaaaatc
    10321 gtagctattt gggcccagga tgaagagggt gtgattggta aggacaatcg tctgccttgg
    10381 catttgccag cagaattgca gcactttaaa gaaacaactc tgaatcatgc tatcttgatg
    10441 gggcgggtaa cctttgatgg aatggggcgt cgcttgcttc caaaacggga gaccctgatt
    10501 ttgacgcgta atccggaaaa aaagatagat ggggttgcta cttttcagga cgtccagtct
    10561 gttcttgact ggtatcaggc tcaagaaaag aatctctaca ttatcggtgg gaagcaaatt
    10621 tttcaggctt ttgaagctta ccttgatgaa gtgattgtca ctcacattca tgctcgggtg
    10681 gaaggagata cctatttccc tgaagagctt gacttgtctc tttttgagac agtttcaagc
    10741 aaattttacg ccaaagatga gaagaatcct tatgatttta ccatccaata ccgcaagaga
    10801 aaggaagttt aatggaacgg agtttatttg gtctattcac ggcttttctg tgtttcatct
    10861 gctttctggc aggagcacaa gcttttcgta aaaagcgtta cgggctttct atcctccttt
    10921 ggttaaatgc ctttaccaat ctggtcaata gtattcatgc tttttacatg accttatttt
    10981 agatagaatg acagtataga atggaaggaa atcatgtcta caaatagaaa aaatgatatg
    11041 atggtttatt gctcattttg tggcaaaaac caagaagaag tacaaaaaat aattgctggc
    11101 aacaatgctt ttatttgtaa tgaatgcgtg gagttagctc aggaaatcat tcgagaagaa
    11161 ttggttgagg aagtcttggc agacttgtct gaggtgccaa aaccaattga actcctccat
    11221 atcttgaacc actatgtaat tggtcaagat cgtgccaagc gtgccttggc agtggcggtt
    11281 tataaccact acaaacgcat caatttccac gatacacgcg aagagtcaga agatgtggat
    11341 ttgcagaagt caaacatttt gatgattggc ccaactggtt cagggaaaac tttccttgcc
    11401 cagaccttgg ctaagagctt gaatgtacct tttgctattg cggatgcgac agctctgacg
    11461 gaggctggtt atgtgggtga ggatgtggaa aatatactcc tcaaactctt gcaggttgct
    11521 gactttaaca tcgaacgtgc agagcgtggc attatctatg tggatgaaat tgacaagatt
    11581 gccaagaaga gtgagaatgt gtctatcaca cgtgatgttt ctggtgaagg ggtgcaacaa
    11641 gcccttctca agattatcga gggaactgtt gctagcgtac cgcctcaagg tggacgcaaa
    11701 catccacaac aagagatgat tcaagtggat acaaaaaata tcctcttcat cgtgggtggt
    11761 gcttttgatg gtattgaaga aattgtcaaa caacgtctgg gtgaaaaagt catcggattt
    11821 ggtcaaaaca ataaggcgat tgacgaaaac agctcataca tgcaagaaat catcgatgaa
    11881 gacattcaaa aatttggtat tatccctgag ttgattggac gcttgcctgt ttttgcggct
    11941 cttgagcaat tgaccgttga tgacttggtt cgcatcttga aagagccaag aaatgccttg
    12001 gtgaaacaat accaaacctt gctttcttat gatgatgttg agttggaatt tgacgacgaa
    12061 gcccttcaag agattgctaa taaagcaatc gaacggaaga caggggcgcg tggacttcgc
    12121 tccatcatcg aagaaaccat gctagatgtt atgtttgagg tgccgagtca ggaaaatgtg
    12181 aaattggttc gcatcactaa agaaactgtc gatggaacgg ataaaccgat cctagaaaca
    12241 gcctagaggt gactatggaa cttaatacac acaatgctga aatcttgctc agtgcagcta
    12301 ataagtccca ctatccgcag gatgaactgc cagagattgc cctagcaggg cgttcaaatg
    12361 ttggtaaatc cagctttatc aacactatgt tgaaccgtaa gaatctcgcc cgtacatcag
    12421 gaaaacctgg taaaacccag ctcctgaact tttttaacat tgatgacaag atgcgctttg
    12481 tggatgtgcc tggttatggc tatgctcgtg tttctaaaaa ggaacgtgaa aagtgggggt
    12541 gcatgattga ggagtactta acgactcggg aaaatctccg tgcggttgtc agtctagttg
    12601 accttcgtca tgccccgtca gcagatgatg tgcagatgta cgaatttctc aagtattatg
    12661 agattccagt catcattgtg gcgaccaagg cggacaagat tcctcgtggt aaatggaaca
    12721 agcatgaatc agcaatcaaa aaaaaattaa actttgaccc gagtgacgat ttcatcctct
    12781 tttcatctgt cagtaaggca gggatggatg aggcttggga tgcaatctta gaaaaattgt
    12841 gaggaaaaga aaatggcaaa aacaattcat acagataagg ccccaaaggc tatcggaccc
    12901 tatgttcaag gaaaaatcgt tggcaacctt ttgtttgcta gcggtcaagt tcccctatcc
    12961 cctgaaactg gggaaattgt aggagagaat atccaagaac agacagagca agtcttgaaa
    13021 aacatcggtg ctattttggc agaagcagga acagactttg accatgttgt caaaacaact
    13081 tgcttcttga gcgatatgaa cgactttgtt ccttttaatg aggtttacca aacggccttc
    13141 aaagaggaat tcccagctcg ttcagctgtg gaggtagctc gtcttcctcg tgatgtaaaa
    13201 gtcgaaattg aagtcatcgc agagattgga taagctagtt gaagtttggt gttgccaaac
    13261 ttcttttgat ataaggagaa aaagatgaca aagaaacaac ttcacttggt gattgtgaca
    13321 gggatgagtg gcgcagggaa aactgtagcc attcagtcct tcgaggatct aggttatttc
    13381 accattgata atatgccgcc agctctcttg cctaagtttt tgcagctggt tgaaattaag
    13441 gaagacaatc ctaagttggc cttggtagtg gatatgcgta gccgttcttt cttttcagag
    13501 attcaagctg ttttggatga gttggaaaat caagatggtt tggatttcaa aatcctcttt
    13561 ttggatgcgg ctgataagga attggtcgct cgttacaagg aaaccagacg gagtcaccca
    13621 ctagcagcag acggtcgtat tttagatgga atcaagttgg aacgtgaact cttggcacct
    13681 ttgaaaaata tgagccaaaa tgtggtggat acgactgaac tcactccacg tgagctgcgt
    13741 aaaacccttg cagagcagtt ttcagaccaa gaacaagccc agtctttccg tatcgaagtc
    13801 atgtctttcg gatttaaata tggaatcccg attgatgcgg acttggtctt tgatgtccgt
    13861 ttcttgccaa atccctatta tctaccagaa ctgagaaacc aaacgggtgt ggatgaacct
    13921 gtttatgatt atgtcatgaa ccatcctgag tcagaagact tttatcaaca tttattggcc
    13981 ttgattgagc cgattctgcc aagttaccaa aaggaaggta agtccgtttt gaccattgct
    14041 atgggatgta cgggtggaca acaccgtagt gtggcatttg ctaaacgctt ggtgcaggac
    14101 ttatccaaga attggtctgt taatgaaggg catcgcgaca aagaccgcag aaaggaaacg
    14161 gtaaaccgtt catgagaaaa ccaaagataa cggtgattgg tggagggact ggaattcccg
    14221 tcattctaaa aagtctgcgg gaaaaagatg tggaaatcgc agctatcgtg acggtggcag
    14281 atgatggtgg ttcttcaggt gaactccgaa aaaatatgca acagttgaca ccgccaggtg
    14341 atcttcgtaa tgtccttgtg gccatgtcgg atatgcctaa gttttatgag aaggtctttc
    14401 agtatcggtt ctctgaggat gccggagcct ttgctggcca tccattggga aatctcatca
    14461 ttgctggctt gtcagaaatg cagggttcaa cctataatgc catgcagtta ttgagcaaat
    14521 ttttccatac aacagggaaa atttatcctt ccagtgacca tcctttgacc cttcatgcag
    14581 tctttcagga tgggacagaa gtggctggag agagtcatat tgtagaccat cgaggcataa
    14641 ttgacaatgt ctatgtgacc aatgccctaa acgatgatac gcctctggcc agccgtcgag
    14701 tagtgcagac catccttgaa agtgacatga ttgtcctagg gccaggttcc ctctttacct
    14761 ctattttgcc caatatcgtg attaaggaaa ttgggcgggc tcttttggaa actaaggcag
    14821 aaattgccta tgtctgcaat atcatgaccc aacgtgggga gacggaacac tttacagata
    14881 gcgaccacgt ggaagtcttg catcgtcacc ttggtcgccc ttttatcgac actgtcttgg
    14941 tgaatattga aaaagtgcct caggaataca tgaattccaa ccgttttgat gaatacttag
    15001 tgcaagtgga acacgatttt gtaggtcttt gtaagcaagt ttcgcgcgtg atttcatcta
    15061 acttccttcg tctggaaaat ggcggtgcct tccacgatgg agatttgatt gtggacgagt
    15121 tgatgcgcat tatacaggtg aaaaaatgag tttcacagta gcagtaaaag aagaaatcct
    15181 gggccaacac catctgagcc ggcatgaatt atctgccatt atcaaaatgt ctggtagcat
    15241 cggtctctcg acttcgggct tgactttgtc agttgtgaca gaaaatgcca aactggcccg
    15301 tcacctctat gagtcctttc tccatttcta tgaaatcaaa tctgaaattc gtcaccacca
    15361 acggagcaat cttcgcaaga atcgcgtcta taccgttttt acagatgaaa aggtgcagga
    15421 tttgttaagt gatttgcact tggcagactc tttctttggc ctggaaacag gtattgatga
    15481 ggcgatttta tcggacgagg aagcaggtcg tgcctatctc tgtggcgctt tcttggcaaa
    15541 tgggagcatt cgtgaccctg agtcaggcaa gtaccagttg gaaatcagtt ctgtttatct
    15601 ggaccacgcg caaggaattg cctcccttct ccaacagttt ttactggatg ccaaggtgct
    15661 tgagcgcaag aagggggctg tgacctatct ccagcgagca gaagacatta tggacttctt
    15721 gatagtaata ggagccatgc aggcacgtga tgattttgag cgggttaaga ttttgcgaga
    15781 aacccgtaac gacctcaatc gggctaataa tgccgagaca gctaatatcg ctcggacagt
    15841 ttctgccagc atgaagacca ttaacaatat cagtaaaatc aaagatatca tgggcttaga
    15901 aaatttgcca gtagatttgc aggaagtggc gcaactgagg attcagcacc cagactactc
    15961 tatccagcag ttagcagata gtctcagcac ccctctgacc aaaagtggtg tcaatcacag
    16021 actcagaaaa atcaataaga tagcggatga attataaaac cacgaatcct atgtgactcg
    16081 tggttctttt ttataaactg gtagagtgtt ttggttgtac tttttgttca gggtcaatgt
    16141 agttgatagc gttgttgaca gcagttggag cttctccgag gcctgtcgca atcagatcaa
    16201 tttttccgtc atagtagcag cagtcaccga tagcatagat acctgcttgg ctggattcct
    16261 gtttgctgtt gacgataatc ttgtgacggt tgaggtcgag cccccagttt ttaaggttac
    16321 cgacagaaga tttgaaacca tagttgacaa agaggtggtc taggtcaatg gtttcagttt
    16381 catcagattt gacttttgtg atttcaagtt tatcaagtgt ttttccattt ccaaggagtt
    16441 ggctaggggc gaatggtgtc ttgatggtta cagatgattc ttgcaaggtt tgaacactgt
    16501 gttccaaggc acggaaatta tctctgcggt gaacaagggt agttggtgcg attttttcaa
    16561 aagccaaagc ccaatccaca gccgagtctc ccccaccaag aatcgtcact ttcttaccag
    16621 cgtattgctg aatgttagaa acgtggtagt ggatattttc atagtcctca accccttcaa
    16681 gttccagcgg acgtggtttg aaggcaccgc cacccatagc gatgataact gttttagtca
    16741 ggtgacttcc tttggaagtt gtgatggcaa attcttcttc ttgtttgtca atctcaagaa
    16801 ccgtttcatt gagatgaata ggggtatcaa atccatttag ctgttcaatc aagcggttag
    16861 tcaactcttc tccagtcagg tttgggaagc ctggtacgtc taggatttcc ttttcagggt
    16921 agagaatagc aggttgtcca cctagctggg gaagagagtc gatgatttga accttggctt
    16981 ggcgtaggtg ggcataaaag gctgcaaaaa gcccgacagg accaccaccc acaatggtaa
    17041 tatcatagag ttgagacata gtttctcctt tgttttttct agtcagttta ttttatcata
    17101 tttttgttca atgtaaaatg gactaggata gggaaaaaaa tgccagaaaa tggtataata
    17161 ggacgaaagt gttttgatag ggaggatatc agggatgaat tttcaacaat tatccaatct
    17221 gcaatattgg accagtttgt ttgcaagtcc atggacgata gctatcaatc tgattgatat
    17281 tttgattgtt gcttatattt tataccattt tacaaaagct attgcaggaa ccaagattat
    17341 gattttggta cgtggagttt tggtgtttat tttagctcaa atccttgcaa atatgattgg
    17401 tttgactacg atttcttggt taatcaatca aattattact tatggggtta ttgcggcggt
    17461 tgttatcttc tctccagaga ttcggactgg tttggaacgt ttgggaagag cgacagattt
    17521 cttttccaat gcccctatta gtgctgagga acagatgatt cgtgcctttg ttaagtctgt
    17581 tgaatacatg agtcctcgta aaatcggggc cttggttgct attcagcgtg tacgtacctt
    17641 gcaggagtat atgtcgacag gaattccctt ggatgctaag atttctgcag aacttctcat
    17701 taacattttt attcccaaca ctcccctaca tgacggtgcg gtgattatca aagaagaacg
    17761 tatcgctgtg acgtctgcct atctgccctt gacaaaaaac acagggattt ccaaggaatt
    17821 tgggaccaga caccgggcgg ctatcggttt atcagaagtc tcagatgcct tgacttttgt
    17881 cgtatcagag gaaacgggag gaatttcgat aacctataat ggaaggttta agcacaacct
    17941 aacacttgat gaatttgaaa cagaattacg tgaaatctta cttccaaaag aggaagtggg
    18001 tcttagtttt aaagaacgat tgctaggagg atggaaacat gaaaaaaaat agtttatata
    18061 tcatatcctc actctttttt gcttgtgtct tatttgtcta tgctacggcg acgaattttc
    18121 aaaacagtac cagtgctagg caggtaaaaa cggaaaccta tactaataca gtaaccaatg
    18181 tccctattga catacgctat aatagtgata agtattttat tagcggtttt gcttcagaag
    18241 tatcagtggt cttgactggt gcaaatcgcc tatcgctagc tagtgaaatg caagaaagta
    18301 cacgtaaatt caaggttact gctgacctaa cagatgccgg tgttggaacg attgaagttc
    18361 ctttgagcat tgaagattta cccaatgggc tgaccgctgt ggcgactccg caaaaaatta
    18421 cagtcaagat tggtaagaag gctcagaagg ataaggtaaa gattgtacca gagattgacc
    18481 ctagtcaaat tgatagtcgg gtacaaattg aaaatgtcat ggtgtcagat aaagaagtgt
    18541 cgattacgag tgaccaagag acattggata gaattgataa gattatcgct gtcttgccaa
    18601 ctagcgaacg cataacaggt aattacagtg gttcagtacc tttgcaggca atcgatcgca
    18661 atggtgttgt cttaccggca gttatcactc cgtttgatac aataatgaag gtgactacaa
    18721 aaccagtagc accaagttca agcacatcaa attcaagtac aagcagttca tcggagacat
    18781 cttcgtcaac gaaagcaact agttcaaaaa cgaattaaaa atagaaaagg attttataac
    18841 aatgggtaaa tattttggga ctgatggagt ccgtggagaa gctaacctag agctaacacc
    18901 agaattagcc tttaaactag gacgttttgg aggctatgtt cttagtcaac atgaaacgga
    18961 agcgccgaaa gtctttgtag gacgtgacac acgtatttca ggggaaatgc tggaatcggc
    19021 cttggtggca ggtctccttt cagtagggat tcacgtatac aaacttggtg tccttgcaac
    19081 accagcagta gcttacttgg ttgaaactga aggagcaagt gccggtgtca tgatttctgc
    19141 tagccacaac ccagcccttg ataacggaat caagttcttt ggcggtgatg gcttcaaact
    19201 agatgatgaa aaagaagcag aaattgaagc cttgctagat gctgaggaag acactcttcc
    19261 tcgtccaagt gcagaaggct taggaatttt ggtagattat ccagaaggct tgcgtaagta
    19321 tgaaggatac cttgtttcaa ctggaactcc tcttgatgga atgaaggttg ccttggatac
    19381 agctaatgga gcagcttcta ccagtgcccg tcaaatcttt gcagaccttg gtgcccaatt
    19441 gacggttatc ggggaaacac cagacggtct taacatcaac cttaatgttg gttcaacaca
    19501 tccagaagcc cttcaagaag tggtcaaaga aagtgggtca gctattggtt tggcctttga
    19561 tggagacagt gaccgcttga ttgctgttga tgagaatggt gacatcgtcg atggtgacaa
    19621 gattatgtac atcatcggaa aatacctttc tgaaaaagga caattggctc aaaatacaat
    19681 tgtgacaact gttatgtcta accttggttt ccacaaggcc ttgaatcgcg aaggtattaa
    19741 caaggcagtt actgcagttg gtgaccgcta cgttgttgaa gaaatgagaa aatcaggtta
    19801 caaccttggt ggtgaacagt ctggtcacgt tatcttgatg gattacaata ccacaggtga
    19861 tggtcaatta tcagcagttc aattgactaa aatcatgaag gaaactggta agagcttatc
    19921 agagttggcg gcagaagtaa cgatttatcc acaaaaatta gttaatatcc gagtggaaaa
    19981 cgtcatgaag gaaaaggcca tggaagtgcc agctatcaag gccatcatcg agaagatgga
    20041 agaagaaatg gcggggaacg gccgtatcct tgttcgtcca agtggaacag aacccctctt
    20101 gcgtgttatg gcagaagcgc ctacaacaga agaagtagac tactatgttg ataccatcac
    20161 agatgtagtt cgtgctgaaa ttgggattga ctaaatccta gaaaactaga tgaaaataaa
    20221 aaattatggt acaatagctt ataatattgt agccgatgga gaaagacatg aatcttaaac
    20281 gtgaacaaga atttgttagt cagtatcatt ttgatgcgcg taattttgaa tgggaaaatg
    20341 aaaatggagc tcctgaaacc aaggtggatg tgaatttcca attgctccaa catgaccaag
    20401 aaaatcaagt gacttccttg attgtcatct tgagctttat gattgtattt gataagtttg
    20461 tcattagtgg aacaatctca caagtaaacc atatcgatgg ccgtattgtc aatgaaccaa
    20521 atgaattgaa ccaagaagag gtggaaacct tggctcgtcc atgtttgaac atgctcaatc
    20581 gtttgactta cgaagtgact gaaattgcat tagacttacc aggaattaat ttggagttct
    20641 aatatgaaat tagctgtttt cacagattcc tctgcttatc tcagtgcaga gatcttgcaa
    20701 agagaagact tgtttgtctt ggatattcct gtcaatattg atggtgagga atatgtcgaa
    20761 ggcatcaatc tgtctgctga ggaattttac caaaaaatgg ctcaggcttc tgaattgcct
    20821 aagaccagtc aaccaagtat tgccaagtta gatgagatct taacttcgct caaagaacaa
    20881 ggctatacac atgccttggg gcttttccta tcttctggaa tttcaggttt ttaccaaaat
    20941 atccagtata tggtcgatga ctatgagggc ttaaccattg ctttcccaga cactttgatt
    21001 acaagtgctc ccctaggcat catggttgaa agtgtcttta attggcgtga tcagggcgat
    21061 gattttgcca gcattcagga taagctagcc attcaaatca gccgtacgtc agcctttatc
    21121 atggtagatg acttggatca tttggtaaaa ggtggacgcc tttcaaatgg ggctgccatt
    21181 ttgggcaatt tgcttagcat taagccaatc ctttatttta acgatcaagg tgtgattgaa
    21241 gtttacgaaa aagttcgtac tgaaaagaag gccaccaagc gcttaattga aattatcaag
    21301 gaaacaacgg cttcaggtca ataccgggtc attgtcattc atgggaatgc tcctgaaaag
    21361 gctgaagaat tgcgtcagca cttgcttgat tttggcttgg gttcggatgt ttcacttgct
    21421 acatttggta gtgtcattgg aacgcaccta ggagcaggaa gtattgctct gggttatatt
    21481 ccagtgattt agttggcgct tctaggctag ttaagatgcc ttgtatctta gaaattgaac
    21541 acggactacc tgcctcttga aaaaagatgc ctgtcttgcc tttcgtggaa agtcagtgcc
    21601 attccctatt tttcagaggc agctaaggtc ctttgtatct tgataattga acacgcctgg
    21661 aaccctgtgt gaaaaagata gttcttccaa gaagtagaca cttcttgatt ggaattccta
    21721 ttttcacttt gtgttcttac ggactttgta tcttagacag gagtagagat gagtattcga
    21781 gtaattattg ccggttttaa gggaaagatg ggccaggctg cttgtcagat ggtattgact
    21841 gatccagact tggacttggt ggcagttttg gatccttttg agtctgagtc agaatggcag
    21901 ggtattcctg ttttcaagga taaggctgat ttagctggtt ttgaagcgga tgtctgggta
    21961 gattttacta ctccagctgt tgcctacgaa aatacacgtt ttgctcttga aaatggcttt
    22021 gctccagtag ttggaacgac tggtttcacg agtgaagaaa ttgcagagct aaaagaattt
    22081 tctcgtgccc aagacttggg tggcctgatt gcccctaact ttgccttggg tgctgtctta
    22141 ctcatgcaat ttgcgacgca ggctgccaaa tatttcccaa atgtggagat tattgagctc
    22201 catcatgaca agaaaaagga tgctccgagt ggaacagcca ttaaaacagc tgagttgatg
    22261 gcagaggttc gagagtccat tcagcaaggt gcagcagatg aggaagagct gattgctggt
    22321 gctcgtggtg ctgactttga tggtatgcgc atccactcag ttcgtttgcc aggcttggta
    22381 gctcatcagg aagtcatctt tggcaatcag ggagaagggt tgaccctccg tcatgactcc
    22441 tatgatcgca tctccttcat gacaggagtc aatttgggaa ttaaagaagt tgtcaagcgt
    22501 catgagcttg tctatggatt agaacactta ttatgagatt aatgcaaatg ccttctgaat
    22561 ttcagaaggc tttaccagta ttagaaaaaa ttaaagaagc aggctttgag gcttattttg
    22621 ttgggggctc tgttcgagat gcccttctcc atagccctat ccacgatgtg gatattgcga
    22681 cgtcttctta tccagaagag accaagcaga tttttccgcg aacagccgat atcggaatcg
    22741 agcatggaac cgtcttggtc ttagatgggg atgaggagta tgaggtaaca acctttcgga
    22801 cagaggatgt ctatgtggac tatcgcagac ccagtgcggt ttcctttgtg cgctcgctag
    22861 aagaagacct caaacgccgt gatttcacag tcaacgcctt tgccttggat gagacaggag
    22921 aaatcgttga cttgttccat ggtttagaag atttggaaaa gcaagtcttg cgagcagttg
    22981 gagtggctag tgagcgtttc aacgaagatg ctttacggat tatgcgtggt ttccgttttc
    23041 aggctagtct tggttttgca cttgagccag aaacatttaa agctatgaag accttgacgc
    23101 cgcttttgga gaaaatttct gtagagcgta ccttcgtcga gtttgataaa ctcttgctgg
    23161 ctccattttg gagaaggggc ttggcttcca tgattgagag tcaagcttat gactatctcc
    23221 ctgatatggc atctagccag gacaagctca acagactgtt tgatttggag actgatttta
    23281 cttttgaatc ctctgaacaa gcctgggcgg ctttactatg ggctttggag attgaaaatg
    23341 cgcagtcatt tttgaaatct tggaagacct cacgccagtt tgccaagcaa gttcaggatt
    23401 tgctgattat tttggctctg cgtgaaaatg gagaattgag taagcgtgat tgttatcgct
    23461 ttgacttgga tttactttta caggctgaaa atcttcgtca agctcaggga aaagaagtca
    23521 acccacaagt cattaaagaa acctaccaga gcttgactat tcatgataag aaagaaatcc
    23581 aaatcaacgg cggtattttg atcaaggaat atggctatca gccaggccca gacttgggag
    23641 agattttaac agagattgag tttgccattg tcgatggaga attggagaat aatcgtgaag
    23701 ccatccatgc ttacctgagg gagaaaaaat gagtgatttt atcgttgaaa aactaagtaa
    23761 atccgttggt gacaagaccg tttttaggga tatttccttt attatccatg acttagacag
    23821 aattggttta atcggtgtca atgggactgg caagaccacc cttttggacg tcctttctgg
    23881 tgtttctgga tttgatgggg atgtcagtcc tttttcagct aaaaatgatt accagattgg
    23941 ttacttgact caggatcctg attttgatga tagaaagaca gttttggata cggttctatc
    24001 tagtgaactc aaggaaatcc agctcattcg tgagtatgaa ttgattatgc tcgactatag
    24061 tgaggacaag caggcgcgtt tggaacgtgt catggcagag atggactctc tccaagcttg
    24121 ggaaattgag agtcaggtca agaccgttct tagcaaattg ggcattcaag acttatctac
    24181 tcctgttggg gaattgtcag gtggtctgag aagacgggta cagttggcac aagtcttact
    24241 tggcaaccac gaccttttgc ttttggatga gccgaccaac catctggata ttgcgattat
    24301 tgagtggctg accctctttt tgaaaaattc taagaagacc gtccttttta tcactcacga
    24361 tcgttatttc ttagacgctt tgtcaacacg gattttcgag ttggagcgag caggcttgac
    24421 agaatatcag ggcaattacc aggactatgt tcgccttaag gcagaacagg acgagcgcga
    24481 tgcagctcta cttcataaaa aagaacaact ctacaaacaa gaactggcct ggatgcgcag
    24541 acaaccgcag gcacgtgcga ccaagcaaca agctcgtatc aatcgtttcc atgatctgaa
    24601 aaaggaagtt tcaggcagta gtgctgagac agacttgact atgaactttg aaaccagtcg
    24661 gattgggaag aaagtcatcg agtttcagga tgtttccttt gcctatgaaa ataagcccat
    24721 tttgcaaaat tttaatctct tagttcaggc tcaagaccgt attggaattg ttggggacaa
    24781 tggtgttgga aaatcaaccc tacttaacct gattgcagga agtcttgagc cgacagcagg
    24841 acaagttgtg attggggaaa ctgttcgcat cgcctatttc tctcaacaaa ttgagggttt
    24901 ggatgaaagc aagcgtgtga tcaattacct gcaggaagtg gcagaggagg tcaagaccag
    24961 tggtggttct acgacttcca tcgctgagtt gctggagcaa ttcctcttcc cacgttcgac
    25021 gcatgggact ttgattgaga aattgtcagg gggtgagaaa aaacgtcttt atctcctcaa
    25081 actgcttttg gaaaaaccaa atgttcttct tttagacgag ccaaccaatg acctagatat
    25141 tgcaactttg acagtcttag agaatttctt gcaaggtttt gcaggtcccg ttttaacagt
    25201 cagtcacgac cgctatttct tggataaggt agcgaccaag attctcgctt ttgaggatgg
    25261 caagattcgt cctttctttg gtcattacac cgactatctt gatgaaaaag cttttgaaac
    25321 agatatggcc aatcaagtgc aaaaggccga aaaggaaaaa gtggtcaagg ttcgagaaga
    25381 caagaaacgc atgacctacc aagaaaagca ggagtgggca agtattgaag gtgatattga
    25441 aaccttggaa aaacgtatcg ctgctattga agaggaaatg caggctaacg gctctgactt
    25501 tggtaagctg gctactctcc aaaaagaatt ggatgagaaa aatgaagcac tccttgaaaa
    25561 atacgaacgc tatgagtatc tcagtgaatt tgatagttaa gactattcga aaatcaaagt
    25621 gcaaactatg tcggcttcac attgccgact caagtacagc ctgtgactgg cttcctaatt
    25681 tgcactttga tttttattga gtataaaaga atagaaaaat cccgtctcat gaaaggattt
    25741 ctctattctt ttttggtttc ttgatgaaag atattttgcc aggtttcgtg gcgacgccag
    25801 atactggtat ggatgatacc atctaactca tagcagatga gtttggtttt ttgactgatg
    25861 gaagtaattt gaatgtcctt gatagcagaa tttaactctt ttttggtctt ataagcctct
    25921 ttatccatct gctctccatc ttggcgaata tagacaaaat catcagtcaa gagttcttct
    25981 agttggtttc cttggtcaat caattgttca cgcataagca attttttata gacattgtct
    26041 aaaatttcat cctcagggat aggtgctggt tcgatatgga catcggtatc aaagacgcca
    26101 aaacgctcct ccagcataga ctcgacctga tccgcgattt catggctttc aaaaacagac
    26161 aagtcaggat tcatctctag tgtaatatcc aggtagatgt tgctaccgta ggtgcgacct
    26221 ctttgcgatt tgaccttgct gattttggga atttccatga tagccttttg gtagtcctcg
    26281 agcaggcggt cgtcaaagcc atctgaaaga ctaaaggaag actcgatgaa gatatcatag
    26341 gcagtcttca agataaagaa agtgatgatg atagcaacca gtttatccac aatcggataa
    26401 ttaaaactac tagctaggat ggcaatggcg gttccaagtg aggtaacagc gtcagaaaga
    26461 ttgtccttag cagctgcctt cagcgccttg gagttggatt tcttactgag gcgagtattg
    26521 tagagataga ccacaaacat aatcgctgca gaaatgattc ctagagttgc accaagagga
    26581 tcaatgaccg tttcttcccg actgagaatc ttttgaatgg tatctcttag aacatcgaaa
    26641 ccgacataga acatgatgat agaagtgatc aagcttgcca aatcttcaat cttccaatga
    26701 ccaaaacggt ggtcacggtc tgcaggctgg cgcgccatcc gaatcccgat taagagggct
    26761 acatttccaa tgatgtccga tacgttatta aaaccatcgg ccaccaaact ggatgaatga
    26821 aggagatgac cagctgctaa tttggctgca gacaagatca aataggtcga aatactgata
    26881 atggctccac gctcagctaa cttgagattt gagatagatt gcttcattca acttcctttc
    26941 tagtcacata gcattctacc attatactgg ttttcaagga aaaattcaaa taaggcagtt
    27001 tttactcttt gaaaactccc caaaatttgg tataatggta ctactcaagg gagtagctgg
    27061 cagaaacctg tgatagtgtc gtcattccga attttatgct gaaaagtatg ctttccggcc
    27121 ctatcttaaa cagcgagact tgttatgatt aacaggtctc tttttgtttg tcgtgaaaag
    27181 atatgacaga aagagagtct gttttgcaca caatgtcaag gaggagacac atgtcaaaag
    27241 aacaaaaacg ccaagcgttt tatactcaga gccctgaaga ggtcttgcag gctgtggatg
    27301 cgaccgagca aggtttgtca tcaagtgagg cggaaaagcg ccttgccgaa tttgggcaca
    27361 atgaactcga agaaggcgag aaacgatcaa tcttggtcaa attcatcgag caatttaagg
    27421 atttgatgat tatcatccta gttgcggcag caatcttgtc agtcgtgact tctggtgggg
    27481 aagatatcgc agatgccatt atcatcctag ctgtggttat catcaacgct gcctttggtg
    27541 tttaccaaga aggaaaagct gaagaagcta ttgaagccct caaatccatg tctagtccag
    27601 ttgcccgcgt tcttcgtgat ggacacatgg cagagattga ctctaaagaa ttggtaccag
    27661 gcgatatcgt tgcccttgaa gcaggtgacg tggtaccagc ggacctacgt ttgatagaag
    27721 ccaactctct taaaattgaa gaagcagcct tgacaggtga atctgtacca gtcgaaaaag
    27781 acttgtcagt cgagcttgcg acagatgctg gtattggtga ccgtgtcaac atggccttcc
    27841 aaaactcaaa cgtaacctat ggtcgtggga tgggtgttgt tgtcaataca ggtatgtaca
    27901 ctgaagttgg tcatattgct ggtatgcttc aagatgcgga tgagactgat acaccactca
    27961 aacaaaattt gaacaacctt tctaaggtct tgacctatgc tatcttggtc attgcccttg
    28021 ttacttttgt agtgggtgtc ttcattcaag ggaaaaatcc acttggtgag ttgttgactt
    28081 ctgttgccct tgccgttgca gccattccag aaggacttcc tgctatcgtt accatcgttc
    28141 tttctcttgg tactcaagtt ttggccaaac gacattccat cgttcgtaag ttgccagcag
    28201 ttgaaacact tggttcaact gaaatcatcg cttctgataa gactggtacg ctgactatga
    28261 acaagatgac agtcgaaaaa gtcttctacg atgcggttct acatgactca gctgatgata
    28321 ttgaactagg tcttgaaatg ccactacttc gttcagttgt cttggccaat gatacgaaaa
    28381 tcgatgtgga aggtaacttg attggtgacc caaccgaaac agcctttatc caatatgcct
    28441 tggacaaggg ctatgatgtc aaaggtttct tagagaaata tcctcgtgta gctgaattgc
    28501 catttgactc tgaccgtaag ctcatgtcaa cagttcaccc attgccagat ggtcgtttcc
    28561 ttgtagcagt caagggtgcg ccagaccaac tcttaaaacg ttgtcttctt cgtgataagg
    28621 ctggggatat tgctccgatt gatgagaagg ttacaaatct cattcataca aacaattctg
    28681 aaatggctca tcaagccttg cgtgtccttg caggtgctta taagattatc gatagtattc
    28741 cagaaaatct cacttctgaa gagcttgaaa atgatttaat ttttactggt ttgattggga
    28801 tgattgaccc tgaacgtcct gaagccgctg aggctgttcg tgtggctaag gaagcgggaa
    28861 tccgtccaat tatgattaca ggtgaccacc aagacacagc ggaagccatt gccaaacgtt
    28921 tgggaatcat tgacgcaaac gatacagaag gtcacgtttt aactggtgct gaactcaatg
    28981 aactgtcaga tgaagaattt gaaaaagtcg ttggtcaata ctctgtttat gcccgtgtgt
    29041 ctccagaaca caaggttcgt atcgtcaagg cttggcaaaa acaaggtaaa gtcgttgcca
    29101 tgacaggtga tggtgtcaat gacgcgccag ctctgaaaac agccgatatc ggtatcggta
    29161 tgggaatcac tggtacagaa gtttctaagg gggcttctga catgattctt gcagatgata
    29221 actttgcgac tattatcgtc gcagtggaag aaggacgtaa ggtcttctca aacattcaaa
    29281 agactattca gtacctactt tctgctaata ctgctgaagt attaaccatc ttcctatcaa
    29341 ccttgtttgg ttgggatgtc ttgcagccgg ttcatctttt gtggatcaac ttggtaacgg
    29401 ataccttccc agctattgct cttggtgttg aacctgcgga gcctggtgtc atgaatcata
    29461 aaccacgtgg acgcaaggca agcttcttct caggtggtgt tttgagttct atcatttatc
    29521 aaggtgtact ccaagcagct cttgttatga gtgtttatgg ccttgcgatt gcttacccag
    29581 ttcatgtggg tgacaatcat gctattcatg cagatgccct aacgatggcc tttgcaaccc
    29641 ttggtttgat tcagctcttc catgcctaca atgtcaagtc tgtctaccaa tccatcttga
    29701 cagttggccc attcaagtct aagaccttta actggtccat cttggtatcc tttatccttc
    29761 tcatggcaac aatcgttgta gaaccgcttg aaggaatctt ccacgtaacc aaactagact
    29821 tgtcacaatg gggaattgtt atggctggaa gcttttcaat gattatcatc gtcgaaatcg
    29881 ttaagtttat tcaacgcaaa ctcggttttg acaaaaatgc gatttaaggg aggtttcgga
    29941 tgtcagcata tcaattaccg accgtatggc aggatgaagc tagtaatcaa ggagctttta
    30001 cggggctaaa cagaccaaca gcaggtgccc gtttcgaaca aaacttgcca aaaggagaac
    30061 aagcttttca gctttattca ctgagaacac caaatggtgt gaaggttact atcttattgg
    30121 aagaattact agaagctggt tttaaggaag cggcttacga cttgtataag attgctatca
    30181 tggatgggga tcaattcgga tcagactttg tgaagctcaa tccaaattcc aagattccag
    30241 ccttattgga ccagtcaggt actgaagatg taagagtctt tgagtctgct catattcttc
    30301 tttaccttgc tgagaaattt ggagcctttt taccaagtaa tcctgtggaa aaggtagaag
    30361 ttttgaattg gctattctgg caagcaggtg cagcaccttt tctaggtggg ggatttggac
    30421 atttcttcaa ttatgctcct gaaaaattgg aatatcctat taaccgtttt acgatggaag
    30481 tgaaacgcca gttggattta ttggataagg aattggctca gaaaccttat attgcaggca
    30541 atgactatac gattgcagat attgctatct ggtcttggta tggacagtta gttcaaggaa
    30601 atctttacca aggttctgca aaattcttgg atgcctcaag ttatcaaaat ctagtaaaat
    30661 gggcagaaaa aattgccaat cgtccagctg ttaagcgtgg cttggaagta acttatacag
    30721 aaattaaata gcaaaaaagt catcttcgga tggctttttg ctacagttga gggaaagggt
    30781 ttgcagttat cggcttttgt gctataattg ctataatata gtaaaaagca agaggagtgt
    30841 gaggaagtgg aaaagaaaat tgtgaaggat atcttatttt tatctcaagt gtctcagccg
    30901 gcaagtcagg aggaccttta tcttgccaga gatttgcagg atacactctt agcaaatcgt
    30961 gatacctgtg ttggtctagc tgccaatatg attggggtgc agaagcgcgt gattatcttt
    31021 aatcttggct tggttcccgt ggtcatgttt aacccagtgc ttctgtcctt tgaaggatct
    31081 tatgaggcag aagaaggctg tttgtccttg gtaggtgtga gatcaactaa gcgttatgaa
    31141 accataaggc ttgcctatcg tgacagcaag tggcaggaac agaccattac cttgacaggc
    31201 ttcccagctc agatttgcca gcatgagctg gatcacttgg aaggacgaat tatttaggag
    31261 gaaagcaaat gaaacgaata gtctttgaac ttatttttat cgcaacgacc tggtatatct
    31321 ttttaccgcc ccttaacctg accagctggg aatttctctt cttcctctgt gggcatttgt
    31381 tagttgtggc aatcttattt ggctttggca aggggataaa ccttgtcaaa acggttcatg
    31441 tgcgccacgg taaggcggaa gctgccttaa atcttgaggg tttcaaaatc aatcggttag
    31501 ggaaaattct gttagcttcg attggaggaa ttcttctctt ggcagctttg gtttccttgg
    31561 taacttctag catgtttcag gctaaaaatt atgccaatgt agtcacggtt acggaaaaag
    31621 actttactga atttcctaag agtgacacca gtaaggttcc tatcctagat agaagtactg
    31681 ctgaaaaaat tggagaccgc tacttgggtt ccctaaccga taaggtgtcg caatacgtag
    31741 cggcagatac ctatacccaa ttgacaattg atgggaaacc ttatcgggtc acaccactag
    31801 aatatgcaga ccctatcaaa tggtttaaca atcaagccaa gggaattggt gagtatatca
    31861 aggtggacat ggtaactgga aatgcggatt tggtggactt gaagacacca atcaagtatt
    31921 cagactcgga gtattttaac cgtgatgtca aacgtcacct gcgcttgaag tacccgacca
    31981 aaatctttaa aactccatct tttgaggtgg acgatgaggg caatcctttc tatgtagcaa
    32041 cggtttacca aaagcaattt ggacttgctg ttcctcgtcc tgcttcagtc attatcttgg
    32101 atgctacaaa tggagaaacc aaggaataca gcttatcaga tgttccagaa tgggtggaca
    32161 ggatctatcc agcagaggaa accattgagc aaattaacta caacggcaag tacaaggacg
    32221 gtttcttgaa tgccatgatt tccaagaaaa acgtgaccca gactaccaag ggctataatt
    32281 acttgtctat cggtaatgac atctatctct acacaggtgt gacgtcggct aatgcggatg
    32341 agagtaatct tggtttcatc cttgaaaata tgcgaacagg agaaatcact aagtatagct
    32401 tggcttctgc gacagaagaa tcagcccgtg aatcagcaga aggtgctgtt caggagaaat
    32461 cctacaaagc aaccttccca atcctcatca acctcaatga caagcctctc tacatcatgg
    32521 gcttaaagga caatgctggc ttggtcaaag agtacgccct ggtagacgca gtcgagtacc
    32581 aaaatgttat cgttgctact acagtggaag agatgctcag caagtatgcc aataaaaacg
    32641 accttgaaat tgacaatgca acgacagaaa gcatcaaggg agtagtagca gacctcaaat
    32701 cagctgttat caagggcgac accgtctact tctttaaagt tgatggcaag atttacaagg
    32761 ttaaggcctc agtatccgat gaccttcctt accttgaaaa tggtagaacc ttcgaaggtc
    32821 aagtaggaaa agacagctat ctcaagacct ttaaagtaca gtaatactct gcgaaaatct
    32881 cttcaaacca cgtcaacttt atctgcaacc tcaaagc
//
LOCUS       DAVLFB010000014        32420 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLFB010000014 DAVLFB010000000
VERSION     DAVLFB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.014"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(106..1740)
                     /locus_tag="VXD58_000569"
     CDS             complement(106..1740)
                     /locus_tag="VXD58_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675314.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1721..2470)
                     /locus_tag="VXD58_000570"
     CDS             complement(1721..2470)
                     /locus_tag="VXD58_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675315.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2649..4103
                     /locus_tag="VXD58_000571"
     CDS             2649..4103
                     /locus_tag="VXD58_000571"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV0675316.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLEEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4193..6811)
                     /gene="alaS"
                     /locus_tag="VXD58_000572"
     CDS             complement(4193..6811)
                     /gene="alaS"
                     /locus_tag="VXD58_000572"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV0675317.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPCITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6833..7318)
                     /locus_tag="VXD58_000573"
     CDS             complement(6833..7318)
                     /locus_tag="VXD58_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV0675318.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7593..7832)
                     /locus_tag="VXD58_000574"
     CDS             complement(7593..7832)
                     /locus_tag="VXD58_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675319.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8093..9163)
                     /locus_tag="VXD58_000575"
     CDS             complement(8093..9163)
                     /locus_tag="VXD58_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0675320.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYK
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9160..9933)
                     /locus_tag="VXD58_000576"
     CDS             complement(9160..9933)
                     /locus_tag="VXD58_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675321.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9930..10736)
                     /locus_tag="VXD58_000577"
     CDS             complement(9930..10736)
                     /locus_tag="VXD58_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675322.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10717..11874)
                     /locus_tag="VXD58_000578"
     CDS             complement(10717..11874)
                     /locus_tag="VXD58_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675323.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12034..12939)
                     /gene="murB"
                     /locus_tag="VXD58_000579"
     CDS             complement(12034..12939)
                     /gene="murB"
                     /locus_tag="VXD58_000579"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV0675324.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13051..13803)
                     /locus_tag="VXD58_000580"
     CDS             complement(13051..13803)
                     /locus_tag="VXD58_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV0675325.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13813..14544)
                     /gene="budA"
                     /locus_tag="VXD58_000581"
     CDS             complement(13813..14544)
                     /gene="budA"
                     /locus_tag="VXD58_000581"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV0675326.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14686..15957
                     /locus_tag="VXD58_000582"
     CDS             14686..15957
                     /locus_tag="VXD58_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV0675327.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15977..16792)
                     /locus_tag="VXD58_000583"
     CDS             complement(15977..16792)
                     /locus_tag="VXD58_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0675328.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16949..17602)
                     /gene="phoU"
                     /locus_tag="VXD58_000584"
     CDS             complement(16949..17602)
                     /gene="phoU"
                     /locus_tag="VXD58_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0675329.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17614..18372)
                     /gene="pstB"
                     /locus_tag="VXD58_000585"
     CDS             complement(17614..18372)
                     /gene="pstB"
                     /locus_tag="VXD58_000585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0675330.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18385..19188)
                     /gene="pstB"
                     /locus_tag="VXD58_000586"
     CDS             complement(18385..19188)
                     /gene="pstB"
                     /locus_tag="VXD58_000586"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0675331.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19199..20083)
                     /gene="pstA"
                     /locus_tag="VXD58_000587"
     CDS             complement(19199..20083)
                     /gene="pstA"
                     /locus_tag="VXD58_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0675332.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20073..20990)
                     /gene="pstC"
                     /locus_tag="VXD58_000588"
     CDS             complement(20073..20990)
                     /gene="pstC"
                     /locus_tag="VXD58_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0675333.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20990..21868)
                     /gene="pstS"
                     /locus_tag="VXD58_000589"
     CDS             complement(20990..21868)
                     /gene="pstS"
                     /locus_tag="VXD58_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV0675334.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22442..23746)
                     /locus_tag="VXD58_000590"
     CDS             complement(22442..23746)
                     /locus_tag="VXD58_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV0675335.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23747..24520)
                     /locus_tag="VXD58_000591"
     CDS             complement(23747..24520)
                     /locus_tag="VXD58_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV0675336.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24510..24788)
                     /locus_tag="VXD58_000592"
     CDS             complement(24510..24788)
                     /locus_tag="VXD58_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV0675337.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24788..25189)
                     /locus_tag="VXD58_000593"
     CDS             complement(24788..25189)
                     /locus_tag="VXD58_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV0675338.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25399..26016)
                     /locus_tag="VXD58_000594"
     CDS             complement(25399..26016)
                     /locus_tag="VXD58_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV0675339.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26006..26779)
                     /locus_tag="VXD58_000595"
     CDS             complement(26006..26779)
                     /locus_tag="VXD58_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV0675340.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEVIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26790..27527)
                     /locus_tag="VXD58_000596"
     CDS             complement(26790..27527)
                     /locus_tag="VXD58_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV0675341.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27532..28662)
                     /locus_tag="VXD58_000597"
     CDS             complement(27532..28662)
                     /locus_tag="VXD58_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV0675342.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28746..29135)
                     /locus_tag="VXD58_000598"
     CDS             complement(28746..29135)
                     /locus_tag="VXD58_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV0675343.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29151..29534)
                     /locus_tag="VXD58_000599"
     CDS             complement(29151..29534)
                     /locus_tag="VXD58_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV0675344.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29535..30323)
                     /locus_tag="VXD58_000600"
     CDS             complement(29535..30323)
                     /locus_tag="VXD58_000600"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV0675345.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            complement(30316..31251)
                     /locus_tag="VXD58_000601"
     CDS             complement(30316..31251)
                     /locus_tag="VXD58_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV0675346.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31381..31557)
                     /locus_tag="VXD58_000602"
     CDS             complement(31381..31557)
                     /locus_tag="VXD58_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV0675347.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31697..32404)
                     /locus_tag="VXD58_000603"
     CDS             complement(31697..32404)
                     /locus_tag="VXD58_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV0675348.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
BASE COUNT         9700 a         7443 c         6216 g         9061 t
ORIGIN      
        1 cctccctata tttttgatta ccaatcttaa tcatttacaa ctacattcta acaaactata
       61 aaagcgtttg tcgaattgaa tttatcaagc aagcaaccaa ctatctcatc ttttttctat
      121 ttctgccaat atgcgtgaca ggtaataatg atagccaaaa atagcaagag caagcaagac
      181 gataagagct cctactccca agttgatggc aaggataggg gagagagact gaaccaagaa
      241 tatgctccca attacaaggg ccatcaggat tgcactataa ataaacaata aaactatggc
      301 gactatgcca tttgaacgat tcaccaggtc cgtaatgcta ctccaattgg ttgacagatt
      361 tttaacgtcc ttaaagtaat ggtggcaaga aaggatgaca ctggcaatga tccagactac
      421 aagaaggtaa atcatcgaaa ggatggacaa gcctagatat agagaaagac cgagcaaagt
      481 cagaactggt aaaaaggact gaacagcaaa gataatccaa aatttcactt tcacataacg
      541 agcaaagtca aagggtaaac tcttcagaaa atcaacattt tccctctcca aggacaaggc
      601 aattgaatgc aggctggtga tattgttatt gacaactgct ataaacactc ctacaaaaaa
      661 caagggtaac cagtatggag gatgaatgtc tggaactatc tgagaatctc ggattttgga
      721 aatcagactg atcatcatga gataaggaag gaaagcactt gtaaaaagca ctgtaatcac
      781 accagtcccc tgtcccaaga gggtgaggtg gtagcgtaaa accatacgga aaaagccctt
      841 tttagtagtt gaaatcctct ccttgctgcg acgttctttt ttgaccttct cctcactgtt
      901 aagcaggatc acgtcataaa aacgaggaag gaccttcttt ttggtcagat aaagcaggaa
      961 gagagttagt cctatccaag cgagcagacc cactaaggct tctgtcgaaa aaggctccac
     1021 tgctattttg taaaagatat gaagaggata aaggagaaat ggaatgtctc taactttgtc
     1081 aacaatactt ccaaaagtcg actgaagaaa gaagataaat attaaaggta tgagaactcc
     1141 tatcccaatc atcacattcg aaaaaataga ctgatacttt ctgaagaccc tagtctgagc
     1201 caagaaatgt actgccacta ccgtcactaa agtaacagag acaaataata aggtcaagga
     1261 cagtagcatc aaaggcaaac ccagccaaag agaaggagct agactaatat agagggctag
     1321 aaaataagct aggattggta caattccagt tagagctggc aagaggacag acagaccttt
     1381 ggccattata atctctgatt ctttaaaggc atagggccta taggaggcca ggtccttact
     1441 ctcataaaag acattgtaaa aggcagtgaa agaagttgaa aaggccagca ccagtaaaat
     1501 agcaaccatc gagctaaaat aaataggcat ttcctcaaaa ggaaaatgaa tggctatatt
     1561 actaaaacag atgagcatca agagactgga aaaaatgtaa gaacttagga ctctagcaga
     1621 aacatttact tttttcccag gattcttagc ctgcttcttt cgtagattag ccagattagc
     1681 ttcttgagat gaatagagga tattgatatc aactaatttt ttaatgacct tgagacgcat
     1741 ccgcaacctc ctcttttcta ccagcaagac taaggtagat actttccaaa aactggtctg
     1801 ggtggtcttt cctcaagtcc tctaccttac cacaataaat caaatgcccc tttttcaaaa
     1861 tggcaatccg atcacagact tgctctgcca cctctaggac atgagttgaa aacaagactg
     1921 tcttcccttt ttgtgcatgt tccttcatca tctgtttcaa atcaaaggca gcctggggat
     1981 ccaaaccagt caagggttca tccaagaccc aaatatcggg atcagacaag agtgctccga
     2041 tgacaaagac tttctgacgc attccgtgag aaagagtttc aataacctga tagcgatttt
     2101 cagcaaaatc aaaaacgttc aatagcctag ctagactagc ctccaagtca gatctactca
     2161 gatcatagga tgaggcgatc aattcccaaa attcattggc cgttaagcgt aaaaataagt
     2221 caggcgagtc tgctacgtag ccaatctttc gtttaatagc caagcgattt tccgataact
     2281 cctgaccgtc taccaaaata cgaccactgc tgggtgaaat gatactgact agggatttta
     2341 tggtggtcga ttttccagcc ccattatgac caatcaagcc cataatctct ccattttcaa
     2401 tctgcaaatt gagattactc aaggcctctt tatcaccata caacttacta acattttgaa
     2461 attcaatcat gggacaactc ctatctttat ctgtattata catcgctata ctagcacttt
     2521 gatacaaaaa aatcaatact ttattttaag tagttaaaat agtttccttc tatcttacgc
     2581 aaacgtttgc accaagagat actttatgat agaataaaga acaagattga caagtaagag
     2641 gaaacatcat gcaaaatcaa acactcatgc aatactttga atggtatctg ccccacgacg
     2701 gtcaacactg gacgcgtctg gctgaaaatg ctccacacct agctcatctg gggatcagtc
     2761 acgtctggat gccaccagcc ttcaaggcaa ccaacgaaaa agatgtcggc tatggggtct
     2821 atgacttatt tgacttagaa gagttcaacc aaaaagggac tgtccgcacc aagtatggtt
     2881 tcaaagaaga ctatcttcaa gccattcaag cccttaaagc acagggaatt caacctatgg
     2941 ccgatgtagt tctcaaccac aaggctgctg ccgatcacag ggaagccttt caggtcatcg
     3001 aagttgatcc tgtagaccgt acagttgaac ttggagaacc cttcaccatc aatggctgga
     3061 ctagttttac cttcgatggt cgccaagata cctataatgg cttccactgg cattggtacc
     3121 acttcaccgg tacagactac gatgccaaac gcagtaaatc tgggatttat ctgatccaag
     3181 gggacaacaa gggctgggct aacgaggaat tggtcgataa cgaaaacgga aactacgact
     3241 acctcatgta tgccgaccta gactttaaac atcctgaagt catccaaaac atctatgact
     3301 gggctgattg gttcatggaa acgactggtg tagctggttt ccgtttggat gccgttaagc
     3361 atattgactc tttctttatg cgcaacttca tccgcgatat gaaggaaaaa tacggtgacg
     3421 atttctatgt ttttggtgaa ttttggaacc cagacaagga agccaatctg gactatctcg
     3481 aaaaaacgga agaacacttt gaccttgtcg atgttcgtct ccaccagaat ctctttgaag
     3541 ccagtcaagc tggcgcaaac tatgaccttc gtggcatttt cacagatagc ctggttgaac
     3601 tcaagcctga caaggctgtg acttttgtcg acaaccacga tacccaacga ggacaagccc
     3661 ttgagtctac cgttgaagaa tggttcaagc cagcagccta tgccctcatt ttgttacgcc
     3721 aagacggcct tccatgtgtc ttttacggag actactatgg gatttcaggg cagtatgctc
     3781 aacaagattt caaagaaatc cttgaccgcc tcctagccat ccgaaaagat ttggcctatg
     3841 gagaacaaaa tgactacttt gaccatgcta actgtatcgg ttgggtacgt tcaggtgctg
     3901 aaaatcaatc cccaatcgca gtccttatct caaatgacca agaaaacagc aagtcaatgt
     3961 ttgtcggtca agaatggact aatcaaacct ttgtagattt acttggtaac caccaaggtc
     4021 aagttacaat tgatgaggaa ggttatggac aattccctgt ctcagctaga tccgtaagtg
     4081 tctgggcagt caataccatc taatagctca taataaccaa gctaggtcca agcggatttg
     4141 gcttttttgt attcacaaaa agacctaccc aaatggatag atctttactt gattacaatt
     4201 tacctgctac tgcatccaac aattcttgga tcttagcttg gttgcttcct cctgccatgg
     4261 ccatgtctgg tttaccacca ccacgtccat cgatgattgg tgctaattct ttgacaaggt
     4321 ttcctgcatg aaggtctttt gtcttgcttg ctacaaggac attgactttg tcaccgatag
     4381 cagcaactag gacaagaaga tcagagtagt ctttttgttt ccagttatct gcaaaggtac
     4441 gaagggcacc ggcatctgat acagacactt gactagcaat gtagcggtga ccgttgactt
     4501 ccttcacatc cttgaagata tcgcctgcgg ctgcagctgc ggctttttct ttcaactcag
     4561 cattttcttt ttgaagttga cgaagttgtt cttgaagtcc ttctaccttg tgaggtactt
     4621 ccttgacttg aggtgctttc aaggttgctg caacagcttt aagagcgtcc tcttgttcac
     4681 gataggcttc aaaggcttcc ttaccagtca ctgccaagat acgacgagtt cctgaaccga
     4741 ttccttcttc tttgacaatt ttgaagagac caatctcaga agtgttgcca acgtgggtac
     4801 caccacaaag ttcaatagag tagtcaccga tagtcacgac acgaacttcc ttgccgtatt
     4861 tctcaccaaa gagggccatc gctcccattt ctttagcagt gtcaatatcc gtttcaactg
     4921 tcttaacttc aagagcttcc cagatttttt cgttgacttg ctgttcaatc gcacgaagtt
     4981 cctcagcagt tactgcttgg aagtgggtaa agtcaaagcg aaggaattca acttcgttaa
     5041 gagatcctgc ctgtgttgcg tggtttccaa ggatattgtg aagggcagcg tgaagcaaat
     5101 gagtcgcagt gtggtttttc atgacacggt gacggcgatt gctatcaatt gccaaggtat
     5161 attcttggtt caaggcaagc ggtgcaagga cttcaactgt atgaagggct tgaccatttg
     5221 gggctttctg aacattggtc acagtagcca caaccttacc tgactcatcc aagatttgtc
     5281 cgtagtcagc tacctgtcca cccatttcag cataaaatga cgtttccgca aagataagag
     5341 aggcagttcc ttctgaaaca gcttctactt ctgcattgtc cgccacgata gctaccaatt
     5401 tagaagacaa ttggctagca ttgtagttga agacactttc tacagtgatg ttttgaagag
     5461 tttcattttg catacccatt gagccaccct tgacagctga ggcacgcgcg cgttcttgct
     5521 gttctttcat ggctgcttca aaaccttcac ggtctacagt cataccagct tcttcagcga
     5581 tttcttcagt caattcaact gggaacccat aagtatcata gagtttgaag acatctgaac
     5641 cagcgataac agattgacct ttttctttca agtctgctac aatgccttgg gcaaagtgtt
     5701 gacctgagtg aagggtacgg gcaaatgatt cttcttcgct cttaacgatt ttctcaataa
     5761 agtcacgttt ctcaagcact tctgggtagt agctttccat gatttttcca acagttggaa
     5821 ccaatttgta aaggaaaggc tcgttgatac ccaatttttg accatgcata gaagcacgac
     5881 ggagaagacg acgaagaaca taaccacgac cttcatttcc tggaagggca ccatcaccga
     5941 tagcaaatga aagagaacga atgtggtctg cgataacctt gaagctcatg ttgtcgccat
     6001 cttggtcata aaccttacca gacaatttct cgacttcacg gataatcggc atgaagaggt
     6061 ccgtttcaaa gttggtctta gccccttgga taacggccac caaacgctcc aaaccagcgc
     6121 ccgtatcaat gttcttatgt ggcaattcct tgtattcgct acgaggaaca gcagggtctg
     6181 cgttaaattg tgacaaaacg atgttccaga tttcaatata acggtcgttt tcaatatctt
     6241 ctgcaagcag gcgaagaccg atattttctg ggtcaaaggc ttccccacgg tcaaagaaga
     6301 tttctgtatc tggtccagaa ggtcccgcac cgatttccca gaagttgtcc tcaattggaa
     6361 tcaagtgact tggatccact cccacttcaa tccagcggtt gtaagaatct ttatcgtctg
     6421 gatagtaggt catgtaaagt ttttcagcag ggaaatcaaa ccattcaggg cttgtcaaaa
     6481 gctcataagc ccaagtgata gcttcgtcac ggaagtaatc cccgatagag aagttcccca
     6541 gcatttcaaa catggtatgg tgacgcgcgg tcttccctac gttttcgatg tcgttggtac
     6601 ggatagcctt ttgggcattg gtaatacatg gattttcagg gataatggtc ccgtcaaagt
     6661 atttcttaag ggttgctacc ccagagttga tccacaaaag agttgggtca tttacaggaa
     6721 ccaaacttac tgatggttct actgagtgac ctttggtcgc ccagaaatca agccacattt
     6781 ggcgtacttg tgcactagat agttgtttca tattgtctcc ttattcactt gtttaatgtg
     6841 attggctttc cagcatttcc acatagtcaa tcgcgacaca gagggaaatg actaggtctg
     6901 cataagcgtc ttcaagaacc gttacggtat aggtagaggt cagatggaag agttccttct
     6961 taatttccgc aatcaactga tcgcgatcat ccagcaattt gaaattcaaa tcccagatat
     7021 tgccctcgat acgaagacct agattatcaa actcatactt atctcgccag aaggtcaact
     7081 tcttacgaat gacaaaactc gagccatccc gaagctgaat ctcaaaacga ggaagcaagg
     7141 tcaagatttc tttactaatc tcactgactt gttcaccagc cgcatcatag atggtaaagg
     7201 ttttaggaat cttaaaaaat gatccctcca cctgataggc aatttctccc ctgtcattct
     7261 tgatagcgaa gcgttcgcct ccaagacgaa acttttgttt gacaagaaat gttttcatca
     7321 acacctccaa aaatcaaaag acaagctcat atcacgaagg gcgaaaaacc gcggtaccac
     7381 cttcattcaa tgaacttgtc attctcttgt tcttatgcaa ttgtatgatt gagtagcatg
     7441 acttcctagc ttagatggct cgcagcaccg ccatttctct ggactaagac aagtgaaaat
     7501 caattctcaa ctttcttatt atatcgtttt tttaatcttg cgtcaactgg aaatgatctc
     7561 cgttgaatta gaccaattcc ctacatctct gattactttt tcaggatata ttttttctta
     7621 ctgccatttt tctttttatc ccaaattttc atattactaa acacagctac tagaatattt
     7681 ccaaatataa aggtgcctat cacccaatat atggactcag ttgttaggta ttgtcgatcc
     7741 aagccatcct ttaaatggaa tagtataaca gtttggttaa caatcataaa ggttggccag
     7801 aaactttttt tgaaaaaagt agacattttc attatttgtt gccgctttct gtaaggttaa
     7861 tactcaataa aaatcaaaaa gcaaactagg aagctagcct caagctgtac ttgagtacgg
     7921 caaggcaacg ctgacgtggt ttgaagagta taggcttagt atactactag gcaagcaaat
     7981 aagcaaataa acaaataaac aactagaata gaaaaagata gggctctaaa aactgacttc
     8041 tattccttaa aaacgaacca gcttgactga ttcgtcttct tacgtttatc tcctacttcc
     8101 gatacatttt aaactgtagg aagaggtcgc tatatttccc tgtccattta tggtcaaatt
     8161 tcttataaac ttctaggtgt ttcatggttt caacatcggg atagaaggcc ttatcttcct
     8221 ttgtttcctc tgggagcaat tccttcgctg gtaggtttgg tgttgaatag ccgacatact
     8281 ccgcattttg gagagcattt tcaggtttca acataaagtt gataaaggca taggctgagt
     8341 tttggttttt aactgttttg ggaatgacca tattgtcaaa ccaaagattg ctggcctctg
     8401 tcggtaccac ataacgtaga ttttcatttt tttctaacat ttggctggct tcaccagaga
     8461 aggtcacgcc gattgcaaca ttattctgaa tcatatagcc cttcatctcg tccgcaacga
     8521 tagccttgat atttggagtc agtttgtaga gcttatccac tgtctcttcc aactgctgca
     8581 gatccttgga gttgaggctg tagccgaggg aattgagtcc tagtcccagc acctcacgcg
     8641 ccccatcaaa gagcatgata gaattcttat actccggctt ccaaaggtca tcccaatgct
     8701 caggcgcttc atctaccatg gtttcgttgt agacaattcc taaggttccc cagaagtaag
     8761 ggatggagaa tttattacct gggtcaaagg actggttgag aaactctggt ccgatatttt
     8821 cgattccttt aatttttgaa taatcaagcg gaaccaagag gtcttcgtcc ttcatcttgt
     8881 taatcatgta ttcacttgga atggcaatat cgtaggtcgt tccaccctgc tttatcttag
     8941 tgtacatggc ttcgttggag tcaaaagtct cgtactgaac ttgaattcct gtttcttctg
     9001 taaactgagt caagagttca ggatcgatat agtcgcccca gttatagata accaattttt
     9061 gactatctcg actattgatt ttactatcta aatgagtcgc aattccccac aagacaagga
     9121 taatcgctgc aattcctgct aaaaatgaat agattttttt catgcttgct cctccttctc
     9181 acgagagata aagtaataac ctacaactag gataatacta aagagaaaga ctagagcaga
     9241 cagggcattg atttctaagg aaatcccctt gcgagcacga gagtaaatct cgactgatag
     9301 ggttgaaaag ccatttcctg ttacaaagaa ggtcacggca aaatcatcca gcgaataggt
     9361 gaaggccatg aagtaacctg caatgataga cggagtcagg taaggaagca tgatttcctt
     9421 aaacatctga aattgactag ctcccaagtc ataggccgca tgaatcatgt cgccattcat
     9481 ttccttgagt cgaggcaaga ccatcaagac cacgatagga atggagaagg ccacgtgact
     9541 agatagaacg gtcaaaaagc caagtgaaaa cttgagttgg gtaaagagaa tcaagaagct
     9601 agcaccaatc ataacgtcag gcgcaaccat gaggatatta ttgagtgata gaaaggcttc
     9661 ttggtatttc ttacgagact ggtagatgta aatggcacca aaagtcccga taatggtcgc
     9721 tatcaaggct gataggaagg ccaagaaaaa tgtctgagcc aaaatcagca tgagtctccc
     9781 atctccaaac atggtttcaa agtgagtcca gctaaaacct gtaaagctat tcatatcatc
     9841 accagcatta aaggcatagc caatcaagta aaaaatcggc aggtagagga ccagaaagac
     9901 cagtcccaga taaaggttgg caaatttttt catcgttctc tcctttcctt ggtcacccac
     9961 atggtgatga acatggtcag gatgagaatc acaccgatgg ttgaacccat accatagttg
    10021 tcattggtta gaaaattctg ctcaatagcc gtccccaagg tgataacgcg gttcccacca
    10081 atcaaacggg tcagcatgaa gagactcaaa cttgggataa agaccgactg aaccccactt
    10141 cgcacaccgt tcatagatag agggaagatg acatgacgga aggtctccca cttagttgca
    10201 ccaaggtcgt aactagcatt gatgagatta ttatccatat cgtctaagac attgaaaatc
    10261 ggcaaaatca taaaggggag ctcgatgtag cttgcgacaa agataaagga aaaatcggta
    10321 aaaagcaact gttgtgaacc aattccgata aattccaaga attggttaat agagccattt
    10381 tgaccaaaaa tcccgataaa agcataggct ttaaggagca aattgatcca ggtaggaagg
    10441 ataatcagca tgagccagag ttgacggtgc ttgagacggg tcaaaaagag ggctgttgga
    10501 tagctgataa gcaaggtcac aaaggtcaca atccctgcat aaagcactga gttgaaactc
    10561 attttgaggt aggtcaagtt ttgtgacgca aagtaagatt tataattttc taaactaaac
    10621 tggccttcaa tgttgaaaaa ggattgtccg aaaatcaaga ccaagggtgc caatacaaag
    10681 agggcaatcc aaagcatgta gggcactaca aagagtttag agcttgtttt cttcatctct
    10741 ttcctcctcg atcgcattga tcagacctgc ttcttgctct tcgatttcca cgtattcttc
    10801 gatacgagca tcgaactctt cttcggtttc attgagacgc atgatgtgga tgtcttctga
    10861 ttcaaagtcc agaccgattt cctcacccac gatggcctta cgggttgagt ggatcatcca
    10921 ttcatttcca agttcgtcat aggcgataat ttcataatga actccacgga agagctgggt
    10981 gtcgacctta acttggagtt tgccttcttc aggaagggta atgcgcaagt cctctggacg
    11041 aataacgacc tcaacaggtt catttggctt catcccacca tcaaccgctt caaagcgttt
    11101 gccgttaaat tcgaccaagt agtcctcaat catggtacct ggcaagatgt ttgactcccc
    11161 gataaaggtg gcaacaaagt ggttgattgg ctcatcgtag atgtccacag gggttccaga
    11221 ctggacaatc tcgccatcat tcataacgaa aatccagtca ctcatggcaa gagcttcttc
    11281 ctgatcgtga gtgacaaaga caaaggtaat gcccaatcgt tgttgtaatt cacgcaattc
    11341 gtactgcatg tctgttctca atttcaagtc cagcgctgat aaaggctcgt ccaacaagac
    11401 cacacggggt tggttgatga tagcacgggc gatggccaca cgctgacgtt gtcctccaga
    11461 aagtttgcgg atggaacgtt tttcataacc ttccaactga accatcttga gaacttccgc
    11521 tacacgctgc tcgatttctt tcttatcaat tttacgcaag cgaagtggaa aggcaacatt
    11581 ttcaaacaca ttcatatgtg ggaacaaggc ataggattgg aagacggtat gtacgtcgcg
    11641 cttgttggtt ggaatatcat tgatacgaac accgtctagc atgatatctc ctgtcgtcgc
    11701 atccagtaaa cctgcaataa tgtttaggat agttgatttc cccgaaccag atgcacctag
    11761 aagggtgtag aatttccctt cttccaactc aaagttgatg tctttgagaa ccttggtgtt
    11821 gctgtcttca aaaactttag agacgttttt gaattcgata attggttttt tcaattggca
    11881 taaattcctt ctttttcata gattaaccga tcggggctct gtcaggtccc cactacctct
    11941 tgcagggagt aaaaccacct gcatacatct tcgctaccga taggctttca ccccctttcc
    12001 atgacatagc agcagctttt caaatacagc ttcctacttg ctttcaccca agatccggac
    12061 ttctctttca agcgtaatac ctgagtgttc cttgactttt tcgataaccg attggatcaa
    12121 gtcctcgtag tctttggccg ttccatctgc gacattgatc ataaatcctg catgcttttc
    12181 tgacacttct acgccaccga tacgatagcc tttcaagcca gcttctgaaa ttaactgacc
    12241 tgcaaaatgc ccgactggac gcttaaagac cgagccacaa gatgggtatt ccaaaggttg
    12301 cttgagttca cgtaggtgcg tcaagcggtc catttcctgc ttgataacct gatgggttcc
    12361 tggggctagg gcaaatttaa ctgacaagac aactgcacca gactcctgaa tagctgaatg
    12421 gcggtaacca aaagccaagt ctttagcaga cagggtttcg atttctccat ccttggtcaa
    12481 gaccttacaa gactgcaaga tgtgagcaat ctcgccacca taggcacccg cattcataaa
    12541 gacagcaccg ccaacgcttc ctggaatacc acaagcaaac tcaaagccag tcaaactatg
    12601 acggagggca atgcgagttg tttcaatcaa gttagcccca gcttctgctt caatggtata
    12661 gccatcaaca gaaacgttat tgagcttgtc acacaagatg acaaatccac gaatcccacc
    12721 atcacgaacg atgatattgc ttgcattgcc aagaaccatc caagggatat tttcttggtt
    12781 ggcaaatttc acaaggcgcg ccaactcaaa acgatttcgt ggaaagacca aataatcagc
    12841 ctctccacct acttttgtat aactatagct atgcaagggt tccttaaaac ggatatcaat
    12901 tccttctaag atttcaagca ttttttctct tacagacatg tcactcttcc ttttacaaaa
    12961 ttcattccat tataccattt ttagagacat ttgacgacca taaaaatacc ttgtttggat
    13021 tttgcataag aaaaagaggt tccccccttt ttatgatttt ttacaaaaga tttccttggt
    13081 tccataggcg accagaacga gctccagtgc tagaatcact tcaaccaaga ctggatttgt
    13141 caaccagcct acttggaaaa gagatggtgc cagatcaaag aaggcatgca agccataggc
    13201 tgctaggaga taaatccatt tcttctggcg aacagcttgg taaacccaaa ctgtcaaaag
    13261 taattggaaa ccaagcgcca agattcgctc aaaaccaagc aaataaatct gccagaccga
    13321 aagtgactga atggttttta acatattttc agacagtaat tgcataacct gtggattctg
    13381 agtttgaact gccgaaagaa cgatgtaaag attgagtaaa ctaataaggc ctagaaaaat
    13441 caactccaag ccaccatgcc ctaggccata ggccaaggca tctgcctttt ccaaacttct
    13501 ctttttctcc aaccatttga agaaaacaag acgagccgtt tcctcaaaaa atgctgccat
    13561 ggctaggcca tagataatat agataagcgg atgatcttgc aagagggcaa tactaccgtc
    13621 tttttgaggg tgcaaaatca agatatgcac cagtttttcc aaaatctgcg aagagacaaa
    13681 gaaagcgact gcacccaagc ccaagacagc tagattaatc tggtatttct ttttggcaaa
    13741 ccaaatgctt cctatcagaa aagctagcaa cagcaacatg gtaataatga tatgaatggt
    13801 cattttcttc tcctattctg ccttttcaat atcttttttc atctcgtcaa cattgaactt
    13861 agcaaacaag tattgacggt cttggactgg gaaacgttgg tccaactggt caactgctcc
    13921 cacctcgata atgccttcct tgatgacaaa atccatgaca tgtccaccga aggtcaaatc
    13981 atctgatatg aagtgcagat ggtagcctgc cacactgacc ccatggaaaa tctcaggcgt
    14041 ccagaaacca acaatagtcc ccgcaacatt gtcacgacta tattccggtt gatgggttgc
    14101 gacatcagca aacttagtat cgggtgttga cttaggaatc atacgcacat gcatatgtga
    14161 aaattctccc cgaatcttga tagaacggaa aagattttcc ccatcataat aagactcaat
    14221 tcgttctttc aattccttgt ctgtcatctc aaagcgctgg cggaaaatga cctctgcctg
    14281 atgcggtacc actgcagcgt aaggaataag ggcatctggt gacacttcca caatttctgg
    14341 ctggtctcct gaccctttgg cctgataagc cttgccatcc aagacaatca attccccatc
    14401 aatagaatcc aaggttccca aaccaaggtc accatgctct agcaattctc ccactgtcat
    14461 ggtaccacca taaaggccag ccatcaaggc tccaagagta ttgtattgaa ataatttcac
    14521 tggttcctgc acgttcttat ccattcactt tcttgtctgt tattctataa atcttactcc
    14581 taagtatacc acatttgccc ctagatgtga acgagagaaa cgctctagac attgccaaga
    14641 aggaaaaaaa agggtacaat gtaacaaaat caagggaggt ctggaatgaa gaaacaaagc
    14701 aagtacaaag aggtcgtttc ctatctgaaa aatggtatcg agtctggacg atttccgacg
    14761 ggtagtcgcc tgccttctat ccgtcaactg agccttgact ttcactgtag caaggacacc
    14821 attcaacgag ccctgctgga attacggcac gaacaatacc tctatgccaa gccccagagt
    14881 ggctactatg tcctagaaca agggcaacac caggacctag aaatcgaggt taccgacgaa
    14941 catgccagtg cctatgacga tttccgactc tgtgtcaatg aaaccttgat tggccgagaa
    15001 aactacctct tcaactacta tgataatcaa gaaggattag aagacctaag acaatccatt
    15061 cacaaactcc tctttgagca agctctctac tgcaaggcta accaactagt actgacttct
    15121 ggaacccaac aagccttgtt tatcctctct caaatatcct ttcctagcca agccaaggaa
    15181 atcttggtgg aacagccaac ctaccatcgg atgaatcgcc tcttgattgc acaggggctg
    15241 gactatcaaa cgattgaacg aggcattgat gggattgacc tgaaggagct ggagcgacac
    15301 ttcaagacag gaaaaattaa gtttttctac accattcccc gatttcacta tcctctggga
    15361 cattcctatt ctgagcaaga caaacgatcc attcttaact tagctgccaa gtatgatgtc
    15421 tatatcgtag aagacgatta tctgggtgat ttggactcca agaagggcca aaccttccac
    15481 tatcttgata cagaggagca tgtcatttat atcaagtcct tctcgaccag cctttttcct
    15541 gcccttcgta ttacagcact cattcttcca aatgctatca aagaagcatt tgtggcctac
    15601 aaaaatatcc tagactacga cagcaacctc attatgcaaa aggccctgtc actctatatt
    15661 gacagtcaat tgtttgaaaa aaatcgtttg gctcgcttga ccaatcatga atcttaccaa
    15721 aaacaaatcg aggaaaggat aactaaaaca ccttgtcccc ttcctcatta tcccctacac
    15781 gatggtttat tgctagacct gagacagtat cctaaaatcg ccagtctcaa acacagtcaa
    15841 ccgggcttgg acttctttga agaggcctat ttaagtacct gtccttatca atttgccaag
    15901 gtgtccctag acaatctgga aaatgtttta aactatttaa aagcagaatt ggaatgacat
    15961 ccaactctgc tttttcttat tcttcaactt cagttagact tgctgctttt tcaagatagc
    16021 tttggtaaat tccttcatcc ttaagttttt ggatgacctt gtctaccact tctttcaaat
    16081 catcactatt ttttctaaga gcaacggcat tggcgtcgcc gtccttcatc ttcaagctga
    16141 cagttgcgac agctaagcca gcgtttttag cagcataact aagtgcaaca ggctcatcca
    16201 tatgaacagc atctactttt ccagcctgca attcattgac tgcttcaccc atattagtta
    16261 gggaagttaa ttgagctttt ggcaattgtt ccttgaccat tgattctgga acagtccctt
    16321 tttgggctgc aatattagca ctttctaggc tagttaaatc cttgtatttt tccacatcag
    16381 ccttacgaac caagaaacta atcttgtttt catagtatgg gattgaaaaa tcaaagactt
    16441 cttttctctc gtcagtagca ctaattcctg caactgctag gtcagcctta ccagtttgaa
    16501 gactggtcaa aacattgtca aaactcatgc ttgagatttc caacttaacc ccaagttcat
    16561 cagcgatagc ctgagccatg tcgatgtctg caccgactac ctggttcttt ccatcaacca
    16621 atgattgaaa ttcaaagggt gcatagtcag gactggttgc cacaactaat ttcccctttt
    16681 gcttaattgc ctcaacagct gactgagaac cattagaacc tgactggcaa gctactaaga
    16741 agaaacttgc aagaaaatta cataaaacaa atatccattt ttttgatttc ataaataaac
    16801 aacctctctg ttaattttgt ataattataa cgatatccaa gttacttgtc aagtgttttt
    16861 taaattttta tctcaaaaat attttttcgt tcaaaaaaag gagccatcag ttgatttcaa
    16921 gctccttttt atacagaatt aaactatttt atagttcgac aatcttacct gtttcaaagt
    16981 agacaaccca ttcacagata tttttagcat agtcaccgat acgctccaag taggaaataa
    17041 cttggaaata atcacgaccc gtaacaatgg cttctggatt tttcttaatc tcttcagtcg
    17101 caaggtcacg gatagtttca aaatagtggt taatttgctc atccatggag gccacccggt
    17161 atgcgtcgtc aacagaacca ttaagataaa gatcaagtgc tgcttcaaca acgcttttaa
    17221 cttcacgtcc cattttttta atttcttcct ctacagctgg aatacgttct tcccccttca
    17281 tacggatggt tgcctgggca atggctacag cgtgatcccc catacgctcc acatctgata
    17341 cagccttaag gacagtcaag actgtacgca aatcttgaga gactggttgt tggagtgcga
    17401 tcatttcaaa tgatttcttt tccagtttca cttcgtattc atttacttct gcatcatctt
    17461 cgatgacctc ttttgccagg tcacggtcat gcgtgacaaa agcacgtacc gtacgattga
    17521 tttgtgagag cacttcttgt cccatagcgt agaactggtt atgtaatttc tctaaatctt
    17581 cttcaaattg agatcgtaac atctttcatc tccttatcca aattttcctg taatatagtc
    17641 ttccgtttcc ttgtgttggg gatcaaggaa catctgcttg gtatcattaa attcaatcaa
    17701 atctccatct aggaaaaatc ctgtcttatc agagatacgt gaagcttgct gcatggaacg
    17761 ggttaccaga agcatggtgt acttgtcttt tagaccatac aaggtttcct caattttacc
    17821 agctgaaatc ggatccaaag ccgaagttgg ctcatccaag aggatgattt taggactagt
    17881 tgccaagaca cgggccacgc agacacgctg ctgttgacca cctgacaatc caatagctga
    17941 atcatataga cgatccttga cctcatccca gatagaggca ccttgcaagg ctttttctac
    18001 ggcttcatcc agaacctgct tatccttaat tccattgata cgaagcccgt agacaacatt
    18061 ctcatagata gtcataggga aaggattagg ttgttggaaa accattccga tttccttacg
    18121 taattcaacc gtatctgtac gcggactgta gatgttgtga ccattgtaca ccacggatcc
    18181 agttgtggtc acctctggat tgagatctcc catgcggttg agagacttga ggagggttga
    18241 cttccctgat ccagatggac caatcaaggc tgtaatttcc ttaggttgga aagataggga
    18301 aacactattc aaagccttct ttttattata ataaacggac aggtctgata cctgtaaaat
    18361 cgcatctgtc atacggtttc ctttctaacc aaagtgacca gatacatagt cattggtgga
    18421 ctgtagcttg gcattttgga aaatagttgc agtcttgtca tactcaatca aatcacccaa
    18481 gtaaaagaag cctgtatagt cacttgcacg agcagcctgc tgcatattat gcgttacaat
    18541 gatgatggta aagtttttct tgagctcaaa catggtctct tctagttgca tggtcgcaat
    18601 cggatccaag gctgaggctg gctcatccat taagaggata tctggcttaa cagagatggc
    18661 acgagcgata cagagacgtt gttgctgacc acctgataag gtcaaggctg acttgtggag
    18721 atcgtcttta acctgatccc agagggcagc ctgacgaagg gaggtttcta cgatttcatc
    18781 taggacttgc ttatccttaa ctccagcacg ttcatgcgca aaggtaatat tacggtaaat
    18841 tgacttagca aatggattgg ggcgttgaaa aaccattcca atgtgtttac gcatttcata
    18901 aacgttgatt tctggacggt tgacatcaat tccacgatag agaatctgcc cagttacttt
    18961 agcaatatca atggtatcat tcatgcgatt gagactgcgt aagtaggtag atttccccga
    19021 tcccgacgga ccaatcaaag ctgtaatttt atttctttca aattgcatat caatcccctt
    19081 aatggattca tttttaccat agtaaacatg gacatcctta gtagaaaggg ctactttttc
    19141 ttcaggaaag gtaaggatat gcttctcatc ccagttatat gttgacatgg cttctccttt
    19201 aggcagcggt taatttcttg tgtagatagc ttccgaactt acgagctcca aagttaaaaa
    19261 tcaggataaa gatcaggagc acagcggcag aacctgctga tacaatggtt ccatctggaa
    19321 tagtgccttc actattgact ttccagatat ggacagccaa ggtttctgct tgacggaaga
    19381 tagagatggg gctagtcaca ctgaggatat tccagttaga ccagtcaaga gctggcgccg
    19441 attgccctgc tgtatagatc agagctgcag cttcgccaaa gatacgacca gatgccaaga
    19501 cgacacccgt tacaatacct ggaagcgctt ctggaataac aacatgaacc actgtctccc
    19561 agcgagaaat cccaagagcc agaccagcct cacgttgggt atggtgaacg tgtttcaaac
    19621 tatcctctac attacgcgtc atctgaggca agttaaagac tgtcaaggcc aaggcacctg
    19681 aaatgattga aaacccatac tcaaactgga ctacaaagat caagtaacca aagagaccca
    19741 ccaccactga tggtaaagag gacaagattt caatacaagt ccgcacaaag ttggtaacag
    19801 gacctttttt agcatattca gccaagtaaa tcccagctcc catagaaaga ggtacagaaa
    19861 taatcaaggt aatgaccaat aggaaaaagg aattgtaaag ctgaatgcca atcccaccac
    19921 ctgcttgaaa agcagaagac cttccagtca agaaagacca agagatatgg ggcaagcccc
    19981 gaaccaagat atagagaatt aaggaagcca agattgtcac aatgatgcta gcaatcgtat
    20041 agaggacagc tgttgcaagt ttatctaatt tcttagcgcg cataattttt ctttcctctt
    20101 tctttcgtaa tcaatttaat cacactgtta aaaactaaac tcatcaagag cagtaccaag
    20161 gccagtgacc agagaacatt attatttaca gttcccatga cagtgttccc aattcccata
    20221 gttaatatag aagttaaagt tgcagctggt gtggtcaagg aagttgggat aacagctgag
    20281 tttccgacaa ccatctggat agctaaagcc tcaccaaagg cacgcgccat cccaaagacc
    20341 actgcagtga aaataccaga acgggccgcc ttcaagatca cacgccagat agtctgccag
    20401 cgagtggctc ccatagcgaa actggcctca cgataataac gaggaaccgc acgcaagcta
    20461 tccgttgtca taaaggttac ggtcggcaaa atcatgacaa agaggacgga aatccctgac
    20521 aaaatcccaa aaccagtccc accaaagaca ctgcgaacaa agggaacgac gacttgcaag
    20581 ccaataaatc cgtacactac tgaaggaatc ccaaccagga gttcaatagc tggttgcaaa
    20641 atcttcgccc cttttggtga tacttcggtc ataaaaactg ctgcaccaat agcaaagggt
    20701 gttgcgataa gggctgagag aatggtaacg ataaaggaac ccaaaatcat aggaagggca
    20761 ccaaattctt tactagaagg attccaagtt cctcccaaaa gaaagtcaaa gatattcaca
    20821 ccattgacaa agaaggtcga caagcctttt tgcgctacga aaaccaaaat catggccaca
    20881 aggatgacta tcaaagaaag acaggcaaag gtcaaacctt ttcctaattt ctccagacga
    20941 gaattctttg atggaagcaa cattttctta gctaattctt cttgattcat tattgtctcc
    21001 cttccaacac tgtcacagtt ccggcagcat ctttttcaac cttcatttcc ttaatcggaa
    21061 tatacttcaa tcctttgaca atcccttctt gggtctcatc cgagagaaca aaattgagaa
    21121 attctgcagc caactcattg ggctgcccca atgtatacat atgctcataa gaccacaagg
    21181 gccaattatt gctacttata ttttctggac ttaagtcata gccattcaac ttcatgcttt
    21241 tgaccgaatc atctatatag gtaagagata aataagagat agctcctgga ctttttgata
    21301 cgattgattt taccgctcca tttgaatcct gctcctgact ttgcatggca gactgacctt
    21361 ccataatgac agtatcaaag gtagcacgag aaccagagcc ggctgcccga ttgataacag
    21421 agatgggtaa gtccttacca ccaacctctt tccaattggt tacctcacct atgaagattt
    21481 gacgaagttg ctctgtcgtt aggttatcaa catcaacctc cttattgaca atcagagcca
    21541 agccagctac cgcgaccttg tggtcaacaa gagcagaagc atcaattccg tctttttcct
    21601 cagcaaatac atctgagttt cctatatcaa ctgccccaga ctgaacctgg gacaagcctg
    21661 taccagaacc tcccccttgg acattgaccg tttttccaac atggatcgtg ccaaattcat
    21721 ctgccgctac ttcaaccaag ggttgcaagg cagttgagcc aacagccgtt atggattctc
    21781 cacgatcaat ccagctagca cagcctacta aacaagccgt cagccaaaaa gcgataagag
    21841 acagagcaag cttttttctt tttttcactg tttttctcct cgaaaataat tatgaatact
    21901 gtgaattttt taagtagttc tttatgagtt gacgcatgaa ttcttaccaa atttctgcgc
    21961 aattgattat ttatataata taggctatat tactctttcc taacctcctt ttttcatatg
    22021 tggataaaat ctcttgtcta tcccttcccc cattgtcacc cattatagtc atttcgtgtc
    22081 tctttttccc ctttttaatg caagggaaat tactctcctt agatgataat ccaaaagcta
    22141 gaaaggtatc tcaaacctct ctactctccc agactagttt acaactaaaa ggaaaagatt
    22201 ctattttatg agaaatctag tttacaagcg gtaagaacgc taataactaa acttcttgta
    22261 ctctttgaaa atctcttcaa accagtgttt tgagctatct atggctagct tcctagtttg
    22321 ctctttgatt ttcattgagt agtaaaacta catgtaatgg caatcaagat atcaagaatc
    22381 atcctactaa aaaaatccat actttcacta taacatagaa taagatattt gactagcatt
    22441 ttcatttgaa tctgaggcct tttggaaaat aatttttcaa aacatttcca gtaacctttg
    22501 caaagcccaa gccattgcct ttaaccaaaa cttggtacca accatttggc agactttctg
    22561 ccagctgaac gatttctcca gccgcatact tgacaaacgc ttcttggcca atttcaaccg
    22621 actgttcgac ctgactcggt ttcaaggcta aaccaagagc gaaactgggc tcaaagcgtt
    22681 tcttcttaaa agtgcccaga tgcagtccat tgcgagcaat cttgagcttc cctaaatctg
    22741 gcaaaagttc tggcaagaga taaagctggt ctccaaaagt ctgcaagata cccggtagat
    22801 tgaccttcaa atggttttgg gcaaattcct gccacaaggc aacttgttca cggctgaggt
    22861 tactcttact tgccttaaat ttaggagctg gattgttacc cttaaactgt agatgggcaa
    22921 caaactgacc ctctccctta aactgatgag ggtacatccg agccgtttct ggcaggtcaa
    22981 taccagctac cattccattg atatgctcta ctggcaacaa gtcaaaatca tactcttcca
    23041 gcaaccaatt gacaatctct tcgttttcct cgggtgccca ggtacaggtc gaataaacca
    23101 gacgaccacc ttcagctaac atggtcactg catcctccag aatttctctt tgcaagctag
    23161 cacattgact cggataatct aagctccaat agtccatagc atcaggttgc ttacgaaaca
    23221 ttccttcacc agagcaaggg gcatcaagaa cgattaagtc aaaatagcct ttaaagacct
    23281 tgaccaagcg gtcggcagat tcattggtca ccacgacatt tgtcgctcca aaacgctcca
    23341 tgttttcaac caaaatctta gcccgtttgc ttgaaatttc attggaaaca agtagcccct
    23401 cccctgctag ataggctgcc agttgagttg atttgccccc cggtgcagca gccaagtcca
    23461 agaccttcat accaggactg ggttgagcta cttgagccac catttgagca gcaggttctt
    23521 gcgaataaac taaacctgta gcatgctcag gcgatttccc tgaaaccttc ccatagtggc
    23581 cccaaggggt ttgagtaatg gcatcagaaa aggaaagttg ctcttctttt aagggattga
    23641 cccgaaaggc cgaaaccgct tcctcctcaa aagaggcaag aaaatctctt gcctcatctc
    23701 ctagtatctc tttatatttt tcaacaaatc cttctggaaa ttgcatttaa gttcttttcc
    23761 tttcgtaaat ataggactga atttcctcct gcatctcaag aggcaccatc atgaccggct
    23821 gtctggtttg aaaatcagga gcttcaccaa aaagggtcac aacccgatag cccagacttt
    23881 cccctaaaat actagctgcg gcataatccc atggttgcag ataagtgaga taggtcaaca
    23941 aacgccctga caaaatcttg gcaaaactaa tggccgcact tccatagaca cgaacaccaa
    24001 gaaccgctcg gctcaaatca gccagccccc attcattggt ttccagcata ccactattcc
    24061 ctgcaatgag aaaatctcca agtggtttag ttttaaaagg agctaggggc ctatcattta
    24121 gacaaactgg aaattcccca ccaccgtggt aacaatcccc tttgaccaca tcataaatca
    24181 gaccaaactg tccctgacca ttttcaaaat aagccatcat aacagcaaaa tcttcctgct
    24241 gggctacaaa attattggta ccatcaatgg gatcaatgac ccaaaccttg ccctcttgaa
    24301 ccgaggctcg cagacaacct tcttcagcac aaatcttatc ctcaggataa cgggacaaaa
    24361 tctcaccaac caagagttcc tgaacttctt tgtccagtct ggtcaccaaa tctgttggag
    24421 aggacttggt ttcaacacgc aagtcttcct gcatatggtc aagaatgtac tgacctgctt
    24481 tcttaacaag ctctttagca aattcaaatt tactttccaa gagaaatctt tccttcccct
    24541 ttttctttgg cagcctgaac tgctcggtaa agtgaatagc cactaaccgt ttcaaattct
    24601 cgtcccaaac gtttctcttc gctcttgctt ggcacgaccg ctttgaatcc cttataggag
    24661 tccagtaact ttttagcctc tactttgcct tcataggcag cttcaacatc attaaaaaaa
    24721 gaaagcactg aagcaagttc ttcagtgctc cacgacaaat ctagtgggta actatactgt
    24781 ttgttcatta actaatacca gctctcattc ttgcttcttt tagttcttgc ttacgataac
    24841 tacgagggag aaaagcacga atctcatctt cattaaaacc gatttgcata cgcttggcat
    24901 caataataat tggacgacgc aaaagactag gatactgctc aatcaaatga agcaattccg
    24961 ataccgaaat actctctaca tcaatattca atttttgaaa aatttttgaa cgagttgaaa
    25021 tgatgtcatc agtaccattt tcggtcaagg aaaggatgtg ttgcaattct tttcttgtta
    25081 aaggactggt cataatattg tgttccacaa agggaacctt atgtttttct aaccaggcct
    25141 tagccttacg acatgatgta cagctcggtg atagaaatag tgtaatcatg cttttctctt
    25201 cttatctata ctttgctact tctattatac aaaaaaataa ggcgcttgac tagggatttt
    25261 tagaaaaaaa gcctattttt tcaagaaaaa taggctgttt gcgaacgatt gacacaattg
    25321 gatttggtta attcactctt aacgatggtt ttaaacgata tatattttta tatatgcaaa
    25381 ttaaaaacat ctttcatttc acttcctacg acttttcaga cacagatagc caaagaagct
    25441 ttcatagagg gcaaaaaaga ggaggaaggc atgaagaaag aaggtctctg gcaaaatcat
    25501 aataacagga tccttggctg gatcaaaaag ccaggtatca tctcccacaa agagaatttg
    25561 atggaaaaga gtaaagaatt ggtcaaaacc aatcaaaact cccccaagtc caatcatcac
    25621 aggtaagact actagagcca ggagactttt tcgataaaga gacaaaaagt cctttttcac
    25681 aatcctattg acaaagacat agaaacttgg cagtgtcact agagctacta gctgaaccaa
    25741 atgaaagaga ttcttgacca ctgcgaaatg gtgcagacca gctgctgacg aacgaaaatc
    25801 aggcatctgt aagacctgac taaaaggatt ggtcagataa ttcatcaaga tatgaaaatt
    25861 gtattgaatg gtttctggtt ttagatagac tcgattcgtt aagtttagcc actgaatctc
    25921 cataggatag aaaatccaag ccagataaat ggtcagaagg attgagaggg agaggagaaa
    25981 gagcatagag ccccaaaagg tcaatttagt tttcatcaaa atcccactcc gcaaggctag
    26041 aaaccacatg tgtcggtgcg attggcaggc cagctacttc ttctgccttg gtaaaacctg
    26101 tcgtcaccaa gagcgttgga atgccattgt caatcccagc ccggatatca gtcaaataat
    26161 tgtccccaac catgattaac tcttcacgtt ccaaacctaa gtgctcaacc gccttgtcca
    26221 taatgatggc atttggtttt ccgatataaa ccggcttcac tcgtgtcgct acttcaagca
    26281 gcgtaatcag tgagccagca cctggcaaaa gaccgcgttc cgtcgggatg ttgaggtcag
    26341 gattggttcc gataaaatgg gcaccctttt gaatagcaag agttgctgtg gcaaattttt
    26401 catagtcgac ttgccaatcc agaccaacta ccacgtaggc aggtttttcc ttgtcttcca
    26461 cataaccagc cgccttgatg acttccttga gtcctgcttc tccgacgaca tagacggtct
    26521 tttcaagccc caaatcattc atatagtcga tggttgccaa agtcgctgtg tagacagtcg
    26581 ataggggcgt atcgatatta aaattctgag ccaacatctc cttaacactc tctggagtgc
    26641 gggttgtatt gttggttaca aagagatagg gaatgtcccg cttttgcaat tcatgaacaa
    26701 aagtctctcc agcagggatt cggtctttcc ccttataaat ggttccgtct aaatcaatta
    26761 aatagccttt atatttcatc tatttctccc taagcctttt ttatttcttg ccaagtaatg
    26821 attgcttggg cattgataac cccatcactt gtaatttcat gcttgctttc cagtccagtc
    26881 cgttcaacag ccgatgtaat caccccacct ggtcgaactt ccttgacata cttgaggttg
    26941 attttcttgg gaatatagtg ggtcaaaaaa tccgctccca tgacctcaaa aatccagtcc
    27001 aagtatttac tgttattgac atgaccattc atatccaagt cgtaaaaacg aacatggtaa
    27061 tccttgctga tcggttcttc caaggactca tacttcggtc cacggataag ttttttatca
    27121 aaatcagact ggtaaggagc cacaatctca ggttcaacaa catggacttt tcgactgtcg
    27181 cggtccatga gaacaaaggt cgccatcatg tggatgagct cctgctccgc ttcattataa
    27241 atagtaaagc gacggtagca aaaaagtcga ttgtagctca aggcttccgt ttcgatggta
    27301 atttcttccg caaaacgagg caaacgaacc acctcaatat catattctac gataatccag
    27361 accagattat attcttccaa aatggcctta tcactaactc ccagttcaat cgactgcatc
    27421 cctgaaactt gcagtgacag caaaatcaca tctggaagtt tgatatgacc gttcatatca
    27481 gccatatcaa aaggaatttt cattttcatt tgataagtta agcccatgat cctactccaa
    27541 aataaatcgt tctgctacag tatctcccaa aaagagacct ctctttgtca tgcgaacgtg
    27601 gtcaccctca atctgcatga ggccttgttg aaccaaatct ctgacaattt ctccataaag
    27661 tccagcaaaa gactgtccaa atttttcctc aaatcgcgcc atggaaaccc cggatttctt
    27721 gcggagtccc aagaacattt cttcttccat ttgctccttt tgactcaggt gatcttctgt
    27781 aatacaagca ttgccttcct caaccgcact gagataatga cgaatgggac catgattttt
    27841 atagcgtact ccattgacat aaccagatgc ccctgcacca ataccatagt attcagcatt
    27901 gtcccagtac atgagattgt gacgactttc aaaaccgggt ttggagaaat tagaaatctc
    27961 ataatgctca aaacccgctc gctccagctc tgcaatgatg tactcaaaca tctccgcttc
    28021 tagttcctcc ttaggcagag gcaatttccc acgtcgcatc cggttcataa agaccgtatg
    28081 gttttctaaa atcaaactat acaaactcat gtggggaata tccagtccaa tggctttagc
    28141 cacattttcc tttacttgct ccatggtctg accaggcaga gcataaatca aatcaatgga
    28201 gatgttgtca aaaccagcca gtttcagacg atcgatattt tcataaatat ccttctccaa
    28261 atgactgcgc ccaatctttt tcaacatctt atcatcaaag gtctggacac ctagcgaaac
    28321 acgattgaca gccgaatttt tcaaaacagc tatcttatcc gcatccaaat cgcctggatt
    28381 ggcttcaatg gtcaactctt ccaagacaga caagtccaag tttttagtca agccattcag
    28441 taacacctcc agttgcggag ccgacagggc tgtcggtgtt ccaccaccga tataaagggt
    28501 tgacaacttt tcaatatcat aagaacgaaa ctcttccagc agatgctcta aatagctgtc
    28561 gactggctga tttttgatga agacctttga aaaatcacaa taataacaaa tctgggtaca
    28621 aaatgggatg tgcacatagg ctgacgttgg ttttttctgc atagtaatta ttataccaca
    28681 aagactagat tccagataaa aatcaccatc cccagataca tagtccgtcc ggagatggtg
    28741 atggtttatt cttctgttat atcaatcaca atctcttctg agtcatcaag agcttcggct
    28801 ttttcttgcc attgttcctt gagattattt aattgatttt ttgatgcttc tgtcgcttga
    28861 aaagcatagg atttagcttg agcaagtata ctgtccacag tgatttcacc tgactcaacc
    28921 tgttcttttg ttttcagaac aaaatctgta gcctgctcct taacttctgt cagtttttca
    28981 cagacttgct ccttggcata ctccggatct tctctcaaat catctagaaa atcttgagcc
    29041 tgactgcaaa cttgtttgcc cttatcactt gttaaaaaca aggcaagagc tgcacctgaa
    29101 accgttccta aaaggattga ggataattta cccataagga ttctcctttt ttattttttg
    29161 aaaaatttac ttgcaagacg aagagctgac agacttgcac cagtcttgag tgtttttgaa
    29221 ccagctgatg aagctttctt gctcaagaca cgcgcatggt cattgaggtc tgaaacagat
    29281 agagataaat ctgcaacagc actgaagagt ggatcaatcg tagccacctt gacattgata
    29341 tcatctgcca agacattgac cttagccaac aactcattgg tgtgatgcaa ggtcacatcc
    29401 acatctgaag tcaaggtttt aatcgtcttt tctgtttcat cgatgacacg accaagcttt
    29461 tgtacagtaa tgatcagata gaccaaaaag acaatcaaag ctagggcaac aagaatatat
    29521 gcaacttcta acatttattt ttcctcctct gtaatatagt aaggggcctt ctttcgattt
    29581 tgataaataa cgatcattat accgagaccg ataaggacaa ctgacagcca ttgggacact
    29641 cgaaggctga agaacatgag actatctgtt cgcatacctt cgatgaccat acgaccgaaa
    29701 ccataccaaa tcaagtaaaa ggccgtgata tgacctcgtc tgagactctt ccatttccgt
    29761 ctaaaaatca gaatcaaagc aaagccaagc agattccata gagactcata aaggaaagtc
    29821 ggttgacggt agctcccctc aatatacatc tggtcacgga taaagccagg tagataatcc
    29881 agattatcca ctgttgcacc ataagcttct tggttaaaga aattacccca acgccccaaa
    29941 ctttgagcaa tcataacgct aggcgccgca atatctagaa aatcccaagt attgatgagt
    30001 ttacggtcag caaagatata gagcacaaga gccccagtta tcaaaccacc gtaaatggcc
    30061 aaaccaccat tccaaatggc aaaaatctct cctaaattct gactatagta atcaaatcgg
    30121 aaaataacat agtagagacg agctcctaaa atagccaagg gaaaggctac taagataaaa
    30181 tctaaaatat cgtctggtat gatcttcttt ctaggtgctt ctttcatggt caaataaacc
    30241 gcaagaatca agcctgtcac aatacataag gcataccaac gaatggctag gggtcctagt
    30301 tgaatagcaa ttggatcaag cattttgcac ctcatttcga gcaatgagac ttgtcagtcg
    30361 ttcgtcgaac aaacgggtcg catcaaagcc catttccttg gcacgataat tcatggcagc
    30421 tgcctcaatc acaacagaga tattacgacc tgttttaact ggaatacgaa tacgaggaat
    30481 ggctacgcca gaaacttcaa gttcctctgc attgtttcca agacgatcaa aggtcttatg
    30541 tgtatcgtaa ttttccaaat agacagcaag ctgaacctgt gaagaatcct tgacagcact
    30601 cgcaccgtag agactcataa catcgataat accaacccca cgaatttcaa tcaagtgttt
    30661 caaaatttca gctggttcac cccagagagt aatctcatcc ttggcaaaga tatcgacacg
    30721 gtcatcggct accaaacggt gaccacgttt gacaagctca agacctgtct cgctcttacc
    30781 aattccacta tctccctgaa tcaagacgcc catcccataa atatccatca agacaccgtg
    30841 cacactggta cgttctgcca aacgagaatc cagatagcta gataactctc cagataaacg
    30901 actggtagct gcacggctgg ttaaaatagc aatcttacat tctctagcag cctttaacat
    30961 ctcctctgga accaccaaac cacgagcaac aatgactgct ggtgtctcag gtagaaacat
    31021 ttttttcaaa acttcataac ggctgttgga aggcatgctg atcagataag accactcctt
    31081 catccccaaa agttgaatcc gctctggtgt atagtagtca aaatagcctg tcatttcaag
    31141 accaggtcgc gtaatatccg ctgtattgat ttccttttca agcaactctg gttcaccata
    31201 gacaatgtct agtctaagct tttcaatcac ttcttttact aaaaccgaca tcatttcctc
    31261 cttcaaatga gttcttctct ctattatatc aaattgtagg aggaagtttc agatttttgc
    31321 aggctttgga gtatttattt aaaagaaaaa ggactagatt tctctagcct ttcatttcaa
    31381 ttagaatttt ttacgtttac gagctacttc tgatttacgt ttacgtttta cagaaggttt
    31441 ttcatagaat tcacgtttgc gtgtttcttg aagagtacca gctttagtaa ccgcacgttt
    31501 gaaacggcga agtgcgtcgt caagagattc attcttacgt actactgttt tagacatttt
    31561 tttcactccc ttcaagttca atatctattt cattctacac gtaaaaggaa taattgtcaa
    31621 gcaaaaatgc ggtttaattt tgattttttc ttccatttat acaagagttg aaagcgttca
    31681 atagaacatt gcttttttat ttttcgagta agctaagcgc ttcagcatct gcgatgatgg
    31741 ttacatcagg gtgattttga aggctacttg caggtaggtt ctcagtcact gggccagata
    31801 ctgttccggc aatggcttct gctttcgact caccgtaagc aaaaagaata atagacttgg
    31861 catccaaaat gtttttaatc cccattgaaa tagcttgggt tgggacgtct tcaatcttgg
    31921 caaagaagcg tgcattggct tcgatagtag actggtcaag ttctactaga tgcgtttgac
    31981 tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc attgcgaccg attcccaaga
    32041 tttggaggtc aactggatgg tcagccaaaa tttggttgta gcgttcaact tcagcttcag
    32101 cattgtcctt aacaccacga ggcaagaaac tttctttaaa tggtttttgg ttgaacaagt
    32161 tttcttgcat gaagtaacga taagattgtg gattgtcccc atcaaggccc acatactcat
    32221 caaggttgac actggttaga tttgaaaaat caaggtcact ctcaacaatt tccttgtaaa
    32281 attcaagtgg gctacttcct gtcgcaagtc ctagagtttg agctccatta gccaattttt
    32341 ccttcaaaat ctcaaaagca acttttccac cttgaacttg gttttcaact ttaataactt
    32401 tcatcttttc atcctccatt
//
LOCUS       DAVLFB010000015        31818 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLFB010000015 DAVLFB010000000
VERSION     DAVLFB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.015"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(410..502)
                     /locus_tag="VXD58_000604"
     CDS             complement(410..502)
                     /locus_tag="VXD58_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV0675349.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            663..1634
                     /gene="ruvB"
                     /locus_tag="VXD58_000605"
     CDS             663..1634
                     /gene="ruvB"
                     /locus_tag="VXD58_000605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV0675350.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1615..2187
                     /locus_tag="VXD58_000606"
     CDS             1615..2187
                     /locus_tag="VXD58_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV0675351.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2418..3176
                     /locus_tag="VXD58_000607"
     CDS             2418..3176
                     /locus_tag="VXD58_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV0675352.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3185..3988
                     /locus_tag="VXD58_000608"
     CDS             3185..3988
                     /locus_tag="VXD58_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV0675353.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4010..5269
                     /gene="rseP"
                     /locus_tag="VXD58_000609"
     CDS             4010..5269
                     /gene="rseP"
                     /locus_tag="VXD58_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV0675354.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5282..7135
                     /locus_tag="VXD58_000610"
     CDS             5282..7135
                     /locus_tag="VXD58_000610"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV0675355.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7235..8614
                     /gene="bglA"
                     /locus_tag="VXD58_000611"
     CDS             7235..8614
                     /gene="bglA"
                     /locus_tag="VXD58_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV0675356.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8806..10614
                     /gene="glmS"
                     /locus_tag="VXD58_000612"
     CDS             8806..10614
                     /gene="glmS"
                     /locus_tag="VXD58_000612"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV0675357.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10733..11782
                     /locus_tag="VXD58_000613"
     CDS             10733..11782
                     /locus_tag="VXD58_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV0675358.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(11882..15679)
                     /gene="spuA"
                     /locus_tag="VXD58_000614"
     CDS             complement(11882..15679)
                     /gene="spuA"
                     /locus_tag="VXD58_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV0675359.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWHLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDTHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVKIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16096..16509
                     /gene="rpsL"
                     /locus_tag="VXD58_000615"
     CDS             16096..16509
                     /gene="rpsL"
                     /locus_tag="VXD58_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV0675360.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16529..16999
                     /gene="rpsG"
                     /locus_tag="VXD58_000616"
     CDS             16529..16999
                     /gene="rpsG"
                     /locus_tag="VXD58_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV0675361.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17423..19504
                     /gene="fusA"
                     /locus_tag="VXD58_000617"
     CDS             17423..19504
                     /gene="fusA"
                     /locus_tag="VXD58_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV0675362.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19608..23999
                     /locus_tag="VXD58_000618"
     CDS             19608..23999
                     /locus_tag="VXD58_000618"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV0675363.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24101..24364
                     /locus_tag="VXD58_000619"
     CDS             24101..24364
                     /locus_tag="VXD58_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV0675364.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24357..24635
                     /locus_tag="VXD58_000620"
     CDS             24357..24635
                     /locus_tag="VXD58_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV0675365.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24656..24808
                     /locus_tag="VXD58_000621"
     CDS             24656..24808
                     /locus_tag="VXD58_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675366.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            24830..26071
                     /locus_tag="VXD58_000622"
     CDS             24830..26071
                     /locus_tag="VXD58_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV0675367.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26081..26311
                     /locus_tag="VXD58_000623"
     CDS             26081..26311
                     /locus_tag="VXD58_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0675368.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26669..27391
                     /locus_tag="VXD58_000624"
     CDS             26669..27391
                     /locus_tag="VXD58_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0675369.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27608..28942
                     /gene="pepC"
                     /locus_tag="VXD58_000625"
     CDS             27608..28942
                     /gene="pepC"
                     /locus_tag="VXD58_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV0675370.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(28998..29909)
                     /locus_tag="VXD58_000626"
     CDS             complement(28998..29909)
                     /locus_tag="VXD58_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0675371.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(29933..30736)
                     /locus_tag="VXD58_000627"
     CDS             complement(29933..30736)
                     /locus_tag="VXD58_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV0675372.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30764..31753)
                     /gene="manX"
                     /locus_tag="VXD58_000628"
     CDS             complement(30764..31753)
                     /gene="manX"
                     /locus_tag="VXD58_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV0675373.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         9058 a         6128 c         7456 g         9176 t
ORIGIN      
        1 gattttggtc ggggaattgg cggggacttt tttagcgaat ataactaaga aataggtctg
       61 tggtcgcttc agctagttca aactcaattt gattgtaagt gactgttaat tgaagaactg
      121 gaactactgt aataatgatt ttgcttgtct agtagtggcg tattaaatga gaagtaacat
      181 gtaagattgt ctcttcgttt acgaaatcaa aatttctatg aaattgatag aatccctaac
      241 gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa gaaaatgtaa acctttgaca
      301 aaattcgtga actatgggat aataaaaagg aattgagtac tagttctata tcataggcta
      361 gaaaagaccc caagctcacg accaaataag cttgaggtct tttttgacac tatttgtcct
      421 tgtttagcca tttatcgact atacgaagaa cgacaccgac cacaattggt ccgatgatag
      481 ttttgagtac taattccatc atgggctatc tcacctcctt tcgtaggcgg tgtagcagtg
      541 tcgtagaata ttataccaca taagtgctaa acttaggagt cacccaatca ggtggctttt
      601 ttgtttgtgg cttggttttt tgatataata gagccatgag tagaatttta gataatgaga
      661 taatggggga tgaggagtta gtagaacgca cgctccgtcc tcagtattta cgtgaatata
      721 tcggacagga taaggtcaag gaccagctac aaatctttat tgaagctgcc aaaatgcggg
      781 atgaagcgct ggatcatgtg ctcttatttg ggcctccagg tctcgggaaa acgaccatgg
      841 cctttgttat tgccaacgaa ctgggagtca atcttaagca gacgtcgggt ccagtcattg
      901 aaaaagccgg agatctggta gctattttga atgagttaga gcctggggat gtacttttta
      961 ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt gctttatagt gctatggagg
     1021 acttctacat cgatattatg attggggctg gtgagggtag tcgtagtgtt catttggagt
     1081 taccaccttt taccttgatt ggtgcgacga ctcgggctgg tatgctctcc aatccgctac
     1141 gggcacgttt tgggattaca ggccatatgg agtattatgc ccatgctggc ttgacagaaa
     1201 ttgtcgagcg gacggcagat atttttgaga tggaaatcac tcatgaggca gcatctgagt
     1261 tggccctacg tagtcgtggg acccctcgta ttgccaatcg tctcctcaag cgcgtgcgcg
     1321 attttgccca gataatgggg aatggggtaa ttgatgatat tattaccgat aaggctttga
     1381 ctatgctgga tgttgaccat gaaggtttgg actatgtgga tcaaaaaatc cttcgtacca
     1441 tgattgagat gtacagtgga ggacctgttg gtctaggaac tctttctgtg aatatcgccg
     1501 aagagcgtga gacagttgaa gatatgtatg agccttactt gattcaaaaa ggttttatca
     1561 tgcggacacg gtctggacgg gtggcgactg ctaaggcata tgagcactta ggttatgaat
     1621 acagtgaaaa ataagcaaga aatcttagag gcttttagag aaaatccaga tatgatggcc
     1681 attctgacga tcatccgaga ccttggtctg aaagactcgt ggttggcagc aggttctgtc
     1741 agaaatttca tttggaatct tttgtcagac aaatcccctt ttgatcatga aacagatata
     1801 gatgtgattt tctttgatcc agatttttct tatgaggaaa ccttattact ggagaaaaag
     1861 ctgcgagagg attttcctca gtaccagtgg gaattgaaaa atcaggtcta tatgcatcag
     1921 cacagtcctc acactgcttc ctataccagt tctcgtgatg ctatgagtaa gtatccagaa
     1981 cggtgtacga cagttggact gcgcttgaat gaagaatcag attttgaact ctatgcacct
     2041 tatggtttgg aggatatttt gaattttcaa gttcgtccaa ctcctcattt cttagaaaat
     2101 gaagaccgaa tggaactcta tcaaacacgt ttatccaaga aaaattggca ggagaaatgg
     2161 aaaaatttga tttttaaaaa tacttaagga aactttaagc tagggagtgt acactaagtt
     2221 cataagttaa gaagacctta acttaaactc ctaaaacttt ttcataataa tctccctata
     2281 aaaataaagt cgcctaatca ggcggcttat ttttttgaaa aatgggcttg gtgcctgaga
     2341 ataaatagct tagtgataga agaaaatggg gaaatatggt ataatgaaac gatagatttt
     2401 tgaataggaa taagatcatg tttggatttt ttaagaaaga taaggctgtg gaagtagagg
     2461 ttccgacaca ggttcctgct catatcggca tcatcatgga tggcaatggc cgttgggcta
     2521 aaaaacgtat gcaaccgcga gtttttggac acaaggcggg catggaagca ttgcaaaccg
     2581 tgaccaaggc agccaacaaa ctgggcgtca aggttattac ggtctatgct ttttctacgg
     2641 aaaactggac ccgtccagat caggaagtca agtttatcat gaacttgcca gtagagtttt
     2701 atgataatta tgtcccggaa ctacatgcga ataatgttaa gattcaaatg attggggaga
     2761 cagaccgcct gcctaagcaa acctttgaag ctttaaccaa ggctgaggaa ttgactaaga
     2821 acaacacagg attgattctt aattttgctc ttaactatgg tggacgtgct gagattacac
     2881 aggcgcttaa gttgatttcc caggatgttt tagatgccaa aatcaaccca ggtgacatca
     2941 cagaggaatt gattggtaac tatctcttta ctcagcattt gcctaaggac ttacgagacc
     3001 cagacttgat tatccgtact agtggagaat tacgtttgag caatttcctt ccatggcagg
     3061 gagcctatag tgagctttat tttacggaca ccttatggcc tgattttgac gaagcagcct
     3121 tgcaggaagc tattcttgcc tataatcgtc gccatcgccg atttggagga gtttaggagg
     3181 aaatatgacc caggatttac agaaaagaac cttgtttgca gggattgccc tggctatttt
     3241 cctaccaatt ttaatgattg ggggcctctt gcttcagata gcaatcggaa tcatagccat
     3301 gctagccatg catgaacttt tgaagataag aggtctagcg accatgacga tggagggcct
     3361 cttgactctc tttgcaacct ttgcattaac cattcccttg gagaattacc tgactttttt
     3421 gccagttgat gggaatgtgg ttgcctatag tgttttgatt tcaatcatgt taggaacgac
     3481 cgtttttagc aagtcttata cgattgagga tgcggttttc cctcttgcta tgagcttcta
     3541 cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt gcaggtttgg acaaggctct
     3601 cttagccttg tgtatcgtct gggcgacaga cagtggtgcc tatcttgttg ggatgaacta
     3661 tgggaaacga aagttagcac caagggtatc gcctaataaa acccttgagg gtgccttggg
     3721 tggtatttta ggagcaattt tagtaaccat tatctttatg atagttgaca gtacagttgc
     3781 tcttccatat ggaatttaca agatgtcagt ctttgctatt ttctttagca ttgctggaca
     3841 atttggtgat ttactagaaa gttcgatcaa acgtcatttt ggtgttaagg attctgggaa
     3901 atttatccct ggacatggtg gtgttttgga tcgtttcgat agtatgttgc ttgtatttcc
     3961 aatcatgcac ttatttggac tcttttaatc aaaagacgga ggaaacgcta tgctcggaat
     4021 tttaaccttt attctggttt tcgggattat tgtagtggtg cacgagttcg ggcacttcta
     4081 ctttgccaag aaatcaggga ttttagtacg tgaatttgcc atcggtatgg gacctaaaat
     4141 ttttgctcac attggcaagg atggaacggc ctataccatt cgaatcttgc ctctgggtgg
     4201 ctatgtccgc atggccggtt ggggtgatga tacaactgaa atcaagacag gaacgcctgt
     4261 tagtttgaca cttgctgatg atggtaaggt taaacgcatc aatctctcag gtaaaaaatt
     4321 ggatcaaaca gccctcccta tgcaggtgac ccagtttgat tttgaagaca agctctttat
     4381 caaaggattg gttctggaag aagaaaaaac atttgcagtg gatcacgatg caacggttgt
     4441 ggaagcagat ggtactgagg ttcggattgc acctttagat gttcaatatc aaaatgcgac
     4501 tatctggggc aaactgatta ccaattttgc aggtcctatg aacaatttta tcttaggtgt
     4561 cgttgttttt tgggttttaa tctttatgca gggtggtgtc agagatgttg ataccaatca
     4621 gttccatatc atgccccaag gtgccttggc caaggtagga gtaccagaaa cggcacaaat
     4681 taccaagatt ggctcacatg aggttagcaa ctgggaaagc ttgatccaag ctgtggaaac
     4741 agaaaccaaa gataagacgg caccgacttt ggatgtgact atttctgaaa aggggagtga
     4801 caaacaagtc actgttacac ccgaagatag tcaaggtcgt taccttctag gtgttcaacc
     4861 gggggttaag tcagattttc tatccatgtt tgtaggtggt tttacaactg ctgctgactc
     4921 agctctccga attctctcag ctctgaaaaa tctgattttc caaccggatt tgaacaagtt
     4981 gggtggacct gttgctatct ttaaggcaag tagtgatgct gctaaaaatg gaattgagaa
     5041 tatcttgtac ttcttggcaa tgatttccat caatattggg atttttaatc ttattccgat
     5101 tccagccttg gatggtggta agattgtgct caatatccta gaagccatcc gccacaaacc
     5161 attgaaacaa gaaattgaaa cctatgtcac cttggccgga gtggtcatca tggttgtctt
     5221 gatgattgct gtgacttgga atgacattat gcgactcttt tttagataat cgaggaatat
     5281 tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa atgccaagcg atgctcaagt
     5341 tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc caagtttcag caggtgttta
     5401 ttcttatcta ccacttgcca accgtgtgat tgaaaaagct aaaaacatca tgcgccaaga
     5461 attcgaaaag attggtgctg ttgagatgtt ggctccaacc cttcttagtg cagaattgtg
     5521 gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt tacaaactaa aaaaccgtga
     5581 aaaatcagac tttatcttag gtccaactca cgaagaaacc tttacagcta ttgtccgtga
     5641 ttctgttaaa tcttacaagc aattgccact caacctttat caaattcagc ccaagtatcg
     5701 tgatgaaaaa cgcccacgta atggacttct tcgtacacgt gagtttatca tgaaggatgc
     5761 ttatagtttc cacgctaact atgatagttt ggatagtgtt tatgatgagt acaaagcagc
     5821 ctatgagcgt attttcactc gtagtggttt agacttcaag gctattattg gtgacggtgg
     5881 agccatgggt ggtaaggata gccaagaatt tatggccatt acatctgctc gtacagacct
     5941 tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt gacgaaattc ctgcagaagt
     6001 gcaagaagaa atcaaggcag aattgctcaa atggatagtc tctggtgaag ataccattgc
     6061 ttactcaagt gagtctagct atgcagctaa cttagaaatg gcaacaaacg agtacaaacc
     6121 aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt gcaacgccag atgttaaatc
     6181 aattgatgaa gttgcagcct tcctcaatgt tccagaagaa caaacgatta aaaccctctt
     6241 ctacattgca gatggtgagc ttgttgcagc ccttctagtt ggaaatgacc aactcaacga
     6301 agtcaagttg aaaaatcact tgggagcaga tttctttgac gttgctagcg aagaagaagt
     6361 ggcgaatgtt gttcaagcag gatttggttc acttggacca gttggtttgc cagagaatat
     6421 taaaattatt gcagatcgta aggtgcaaga tgttcgcaat gcagttgtcg gtgctaacga
     6481 agatggctac cacttgactg gtgtgaaccc aggccgtgat tttactgcag aatatgtgga
     6541 tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga caaggtgtcc ttaactttgc
     6601 gcgtggtatt gagatcggtc atattttcaa actcggaact cgctactcag caagcatggg
     6661 agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc atcatgggat gttacggtat
     6721 cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac gctcgcctct ttgttaacaa
     6781 aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat ttccctaaag aattggcacc
     6841 atttgatgtg catttgatta ctgtcaatgt caaggatgaa gaagcgcaag ccttgacaga
     6901 aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc ttgacagatg accgtaacga
     6961 acgtgtcggt gttaaattta gcgatagtga cttgattggt cttccaatcc gtatcactgt
     7021 tggtaaaaaa gcggccgatg gcattgtaga agttaagatc aaggcaactg gtgacaccat
     7081 tgaggttcat gcagataacg tgcttgaaac gcttgaaatc ctaagcaaga aataaaaact
     7141 ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt taccttgttt tttctgtata
     7201 atgggagaaa tgagtagata aagaggtaaa tgttatgcta agatttccaa aggattttgt
     7261 ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg acggtaaggg
     7321 agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata atgggattgg
     7381 ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc ttttgttaga
     7441 gactggtcac acagtctttc ggacttctat tcagtggtca cggatttttc cacaaggctg
     7501 tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg ctattaaggc
     7561 taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg cccttcaaga
     7621 ggatggtgat ggttgggaaa ataaggcgac agtctcagcc tatgaagact atgctcgttt
     7681 ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg agcccatcgt
     7741 tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg atgcagaggc
     7801 agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta aggcttgcca
     7861 tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg cttatccacg
     7921 tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc aggcccaatc
     7981 tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa tcttgcatga
     8041 acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcgtg ataatacggt
     8101 ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc gatttgctaa
     8161 gcatccagat agtccactct taccagaaca tttttacgag ccttatgtga tgcctggacg
     8221 taaaatcaat tctcaccgtg gttgggagat ttatgagcaa gggatttatg acatcgccca
     8281 aaatatcaag gaaaattatg gcaatattga gtggatgttg actgagaatg gtatgggtgt
     8341 tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc gtattgactt
     8401 tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca actgtaaggg
     8461 ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata aaaatcgcta
     8521 tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat caggccactg
     8581 gttcaaagaa ttaagcgata ataatggatt ttaaaaagga ctctgactga ttatcccaat
     8641 cggtcagagt tttttgctta caggagctta atttgaacta taccaatttt tactcttgac
     8701 aagtgaaaga aacaagtata tactgttttt gctgattcta taaaagatga attagactat
     8761 accaatttta aggagaaagc acagcttgcc tgtgtcgtat atactatgtg tggaattgtt
     8821 ggtgttgttg gaaacacaaa tgcaactgat attttgattc aagggcttga aaagcttgaa
     8881 taccgtggct atgattctgc gggaattttt gtcctagatg gtgctgataa ccatttggtg
     8941 aaggctgttg gtcgtatcgc agaattgtct gccaagacag ctggtgttga gggaacaact
     9001 ggtatcggac atactcgttg ggcaactcat gggaaaccaa cggaagacaa tgctcaccca
     9061 caccgctctg agacagaacg ttttgtcttg gttcacaatg gggtgattga aaactacctt
     9121 gaaattaaag aagaatacct tgctgggcac cacttcaaag ggcaaacaga tacggaaatc
     9181 gccgtacatt tgattggaaa atttgcggaa gaagacggtc tctcagttct tgaagccttt
     9241 aaaaaagctc ttcatattat ccgtggttca tatgcctttg ccttgattga ctctgaaaat
     9301 ccagatgtca tctatgtagc gaaaaacaaa tctccacttt tgattggtct tggggaaggc
     9361 tacaatatgg tctgctcaga tgctatggct atgattcgtg aaaccaacca atacatggaa
     9421 attcatgacc aagagttggt aatcgtcaag gctgatagcg tggaagttca agactatgat
     9481 ggtaacagtc gtgaacgtgc tagctatact gcggaacttg acttgtcaga tatcggtaag
     9541 ggaacttatc cttactacat gcttaaggaa attgatgagc aaccaactgt tatgcgtaaa
     9601 ctcattcaag cctacacgga tgatgctggt caagtagtga ttgatcctgc tatcattaag
     9661 gctgttcaag acgcagaccg catctacatc cttgcagctg gaacatctta ccatgcagga
     9721 tttgcttcta agaaaatgtt ggaagaattg acagatacac cagttgaact tggcatctca
     9781 tctgagtggg gctacggtat gccacttctc agcaagaaac cactcttcat ctttatcagc
     9841 caatctggtg aaacagcgga tagtcgtcaa gttttggtca aggctaatga aatgggaatt
     9901 ccaagcttaa cagtgacaaa cgttccaggt tcaaccctct cacgtgaagc caactatacc
     9961 atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa ctaaagccta tacagcgcaa
    10021 atcgcagccc ttgccttcct tgcaaaagca gtcggagaag caaatggtaa tgctaaagcg
    10081 caagcctttg acctggttca tgaattgtca atcgtagctc agtctatcga atcaactctt
    10141 tcagagaaag aaaccattga agccaaggtt cgtgaacttc ttgaaacaac tcgtaacgcc
    10201 ttttacatcg gacgtggtca agattactac gtagccatgg aagcaagtct caaactcaaa
    10261 gagatttctt atatccagtg tgaaggtttt gcggcaggag aactcaagca cggaaccatt
    10321 gccttgattg aagaaggaac acctgtcttg gctctcttgt cagatccagt ccttgccaac
    10381 cacactcgtg gaaatatcca agaggtggca gcccgtggtg ctaaggtcct cactatcgca
    10441 gaagaaaatg ttgctaaaga taccgacgat atcgtcctta cgactgtaca cccttacctc
    10501 tcaccaattt caatggtcgt accgacgcaa ttggttgctt actttgcaac cctccaccgt
    10561 ggcctcgatg tggataaacc acgtaacctt gctaaatcag taacggtaga ataagctaaa
    10621 aaagtctagt tatctaggct ttttctaatg agcaaattgg ttgcttgtac tcaagattcg
    10681 tgttagaata atttttcaaa ttgaaggaca gagatagaca aggagaatca caatggtaga
    10741 attgggaatt tcaacatttg gggaaataac ggagcttgaa gggactggac aaacttacag
    10801 tcatgctgaa cgcattcgcc agttggtggc agagattgag ctggctgaca aggttggttt
    10861 ggacgtatat gggattggcg agcaccatcg ggcggatttt gcagtatcag ccccagagat
    10921 tgtcctggcc gctggggcag tcaataccaa gaaaatccgt ttgaccagtg cagtcagcat
    10981 tctgtcaagc atggatccga ttcgtttgtt ccaacagtat gccactatcg atgctttgtc
    11041 aaatggacga tcggagatta tggctggaag gggctctttc acggaatctt tccctctgtt
    11101 tggttatgac ttgaaagact acgactctct ttttgatgag aaattagact tgcttcagtt
    11161 agtcaatgaa aagaccaagt tagactggca aggtcgattg actcaaacga ttgctggtaa
    11221 agaagtttat cctcgcccag ttcaggacaa attgcccttg tggatagcta caggtggtca
    11281 tgtcgaatca acagtgaaga ttgctcaggc tggtctaccg attgtatatg ctattattgg
    11341 tggtaatcca cgttatttta aaaagttgat tcaggcttat cgtgagattg gaagcgaagc
    11401 gggccatgct gacaaagact tgaaagtggg agcccattct tgggggtgga ttgcggaaga
    11461 tggtgagcag gcggtgaaag attatttcca tccgactaag caagtggtgg atgctatttc
    11521 caaagaccgt ccgcactggc aggaattgcg ttatgagcaa tatttggagc aggtagggcc
    11581 aaatggcgcc atgtttgttg gcaatccaga tcaggtggcc gaaaaattga ttcgcatgat
    11641 agaggattta ggattggacc gcttcatgct tcatctaccg cttggttcta tgcctcatga
    11701 ccaggttctg agagctattg aactcttcgg aacacaagtt gcacccaaag tacgagctta
    11761 ttttgctatg aaagaggctt aataaaaaat cctaatcaag tttattagga caacaaatat
    11821 tgtacaaaaa ttaatccccc gagctataat gcttggggga tttttctata ggagggctag
    11881 tttagttctc ttttttattt tttagtaaga aaccgagacc aaggagcgca aggaggctga
    11941 ttcctgcaaa taggagtttg ttttcgtttt tgattcctgt atttggaagt tcagcttgtt
    12001 tatctggggt tgcaggtata ttttccttgc tagagttttc taccgattcg tttcgaaccg
    12061 cttcttttac agatgcttct tgtgcttctt gtgctggttg ttcagcttgt gaatcagctg
    12121 tagcttggct aggtttattt tccgcatcag ctactttggc atctggttta gtagaatcag
    12181 gtctagcttc tggagctggg tcttgatgtg cagggtgaga agcttcattt tgatgttcag
    12241 gcttggaagg ggttgagtct ggtttctctt ctgcagttga ctcatggcta gttccatttt
    12301 gagagactcg aagaacagta gctgtaaggg cattcaattt caagcctttt tcagtccatt
    12361 caagtccttt cgggttggca attttgactg atcctgcttg gttttcatct gccaaaactt
    12421 ccgcatttct tagatgtgca aaggcagttc ccaaattaaa ttcgcgagct ttttcatccg
    12481 cattgacaaa gactgcgtag atatcgccgt ttggagcagt gatttggtag ccaatcacta
    12541 catcctcttt ttccacacca ttttggcctg ggacagtgat gaggtggaca cggtctttga
    12601 tatcttggag actcttaagt cggaaggcat ctgtagattg acgaagggca atcaaacctt
    12661 tcatatagtc acggctcttg acattttcag gataagcttt accatctgta gccttagtcc
    12721 agtcaaactt gttgactgcg tcgctagaat cgtaagaatc atggatgaag taaggatagt
    12781 caaatgggtt gccgtcctta tcacgcaaca agtgagattt gtttggaacc ttatcctctg
    12841 ctactggagt cttgtaggct gggtcacgga attgtttagt acgtccatat tcctgaccgg
    12901 agtggataaa tggagttcct tgagctgtca agaccatgag atttccaagt cgtaaacgac
    12961 ggtggatttc agcatagttc tcagccttgc ttgggtcttt tttgatagac tgggcaatga
    13021 tgtcaaagag ggtcaagtta tcatgggctg ctatgtattg gatgacatct ccagggctgt
    13081 cagcttcaaa gttagttggt tgagcaatga gatttttaaa gatggtgttg acatcacgct
    13141 tgccacctgt gataaaggca ggttgacctt cgtttggata accagatttg aggttgttac
    13201 ggatgtcatc tgaaaagaca gcgacagtat cggtatgttt catccaatct tggtcagcag
    13261 ctttagtagg catgttttca tcaccggcat aggttctcca accttcacca agcatgatga
    13321 ggtttggatt gagggcgcgt gcagccttgt aagcttcttc gatagaagcg gcgtcatggt
    13381 ctcccatcat atcgaaacgg aatccatcca ctttgtaggt atcaactagg tatttgatag
    13441 agtcaactag gagccgtttg gtcatatggt gggttgttcc caagcgtcca ccaccaaagc
    13501 tagttcgagg tgtgccatcg gcatccataa agtggtagta gtttggctct aggtcttcaa
    13561 agatatcgac tttggctgtg tggttataaa cgacatctag gatagctccc ataccacgtt
    13621 tgtggatttc gttgatgagg tttttaaatt ctgcgattcg tttttctgga ttcttaggat
    13681 cgcttgagta cataccagtc aaggagaagt agttttgagg gtcatatccc cagttgtagt
    13741 tgctgttgct tgaagcgtag tcagacaagc gttcatggtt tttcaattca ttgacaaagt
    13801 agtaagacaa gactggaagg agctggatat gggttacacc caagtctttg agatagtcta
    13861 gtttttcaat gaaggcttca aaagtcccaa atggtttggt caagtctttt gcaatggcag
    13921 gatctgaagt gaagtcacgc acatgagctt cgtagataac ggcgtcttca cgagtcttga
    13981 aattgtgaat cttaccataa gtcaagtctt gaggtccgag tttagctgga tctacaaagg
    14041 cggctttagc cactttatgg gtatcgtcaa ttttggcatc gtcgctattc caagcagcaa
    14101 gagatttagc gtaaggatcg agtgcaagaa cagttttacc ttgacgctcg atttggtatt
    14161 gataatagta gccagtgaaa tctgtgattc cgagtttgtt tgtgctgtct agagtttgtt
    14221 tccaagttcc tctttcccct ttttcgagag cgacagttcc aactactttg tcagggtcat
    14281 tcttgtcgta gacaacaaca gaaaccttat cagcacttgg tgaccaaagg gtcaaatcaa
    14341 cttgttttcc ttcttctttt aggtcagctc ccagtttgcc atcatagctg tatgtctcat
    14401 ctttcaggtg ccagcttgtt ttggtagtga attggtcgga attgtagcta acagtataag
    14461 ggtgttttgt gtcagagaaa tctccgctgt aggtcacttt cttaccagct tcatcgattg
    14521 caacatcggt aatagttacc ttgtttccta ggtgattagt gatgttggag tgtttgagga
    14581 tatcttcttt tttagcaccg acaagtgttg aaaagctact ttcaatgcta gaagtgccta
    14641 cgtgttgggc tcctgtcata cggatatcat ggacatagta tggatttgtg taaatcgatt
    14701 catcatcgtc ttttaggaaa atttggctat gatttttcaa atctgtgaac ttataatttt
    14761 ctttacggat tttcacgtcg tctccttgtt tgctctcatc tagtaataaa aatccaaatt
    14821 ctcttgcggc ttcattaaga ggaatgtcga tatagcggcc atatttgcct gtagccgtaa
    14881 agtctgttcc gtcaggccat tgagcgctac ttggattttt cacatctccc cagtaccaga
    14941 gagatttctt gtcatagttg ccatctgtgc ggtagtagtt gacgcgaaca gttcctgcag
    15001 gctgtggctc gtaagagaaa accttgtaat cttggtctaa ccaagcttcg ttcatttttg
    15061 gaactagttt ttctacagat ttatcgccgg ttagattttt tccagctgta ttgttgatga
    15121 ggaagctaat tttcttggct tgttctccct ttaatttgac atctaggtaa tagccgtagt
    15181 catctttctt ggcatccttg aaggacaaag ctccgtttgg ccagttttca gatggttttt
    15241 caacatcgtc ccaagtccat agtccttgag catccttgtt ttcttcagga agttttttga
    15301 catggatacg gaagtagtta tcttcgatgg gctcttctgc cttagtttca gttgttactg
    15361 gactagtaga agtagttggc tctgttgcag gtttttcttc tgttttctct agggaagcgt
    15421 ttgcatttct aggtgaagaa atatcacttt cgttcttacc agagccagct gctgtatcag
    15481 taagaggttg ggcaagggtg gttgtatttc tgttgttggg ttcgttagtg gttggtgtac
    15541 tttcgtcgga tgagatagtt ggtgtagcca tggcaagcaa gacaaggctt gcgccgataa
    15601 ggacagaacc agttccattt ttgagggaac ggatgctgta gaccattttt ttctcagtgt
    15661 gagatggtgt ttttctcata atgattctcc aatcaataaa tttctatatc agtatagcat
    15721 gtagtaaaac aatatgcaag cgttttcttt gatttgtgaa aaaaattctt acttttgaga
    15781 agttttctgt actattttat atatcagtat aaatgacgga gataaaaatg tcacataagc
    15841 caagccgtat tattaggaag atgatgtgtt cccagctatt tttattcaga aattgatctc
    15901 taacattacc aataaaaaag aaaaatatct tgacaagcaa gggaaaattt tgttacaata
    15961 atagacggta ctttttactt ttggtctctc aagagtgtac agggacgtgc tgacaaatgt
    16021 tgcaaaagta cacacagatg atatctgtca ccaagtgtat catcaccaaa aataaaaaaa
    16081 cacaggagaa tgtagatgcc tacaattaac caattggttc gcaaaccgcg taaatcaaaa
    16141 gtagaaaaat ctaaatcacc agctttgaac gttggttaca atagtcataa aaaagttcaa
    16201 acaaacgttt cttcaccaca aaaacgtggt gttgcaactc gtgttggaac aatgacacct
    16261 aaaaaaccta actcagccct tcgtaaattc gctcgtgtac gtttgagcaa ccttatcgaa
    16321 gttactgcct acatcccagg tatcggacac aacttgcaag agcacagcgt ggtgcttctt
    16381 cgcggtggac gtgtaaaaga ccttccaggg gtacgttacc atatcgtccg tggtgcactt
    16441 gatactgcag gtgttaacga tcgtaaacaa ggccgttcta aatacggtac taaacgtcca
    16501 aaagcataag gaaaggggat aaagagaaat gagtcgtaaa aatagagctc caaaacgtga
    16561 cgtattgcca gatccgctat acaattcaca actagttact cgtcttatca accgcgttat
    16621 gcttgatggt aaacgtggta ctgctgcttc aatcgtttac ggtgcttttg agcaaatcaa
    16681 agaagctact ggcaacgatg cacttgaagt atttgaaaca gctatggaaa acatcatgcc
    16741 tgtacttgaa gtacgtgcac gtcgtgttgg tggttctaac taccaagtcc cagttgaagt
    16801 tcgtccagaa cgtcgtacaa cacttggact tcgttggttg gtaacaatcg ctcgtcttcg
    16861 tggtgaacac acaatgcaag accgtcttgc aaaagaaatc ttggatgctg ctaacaacac
    16921 tggtgcagca gttaagaaac gtgaagatac tcaccgtatg gctgaagcta accgtgcatt
    16981 cgcacacttc cgttggtaag ataggatgcg aaagcgttaa gaaagtccca gagaaaatag
    17041 ggaatcgaag caggttgcgg ttgcaaccaa tgagattcat ctttttctcc agacttttag
    17101 cttgagctca actaaatcat gatgctaggc acggaaggat gcaaggtgaa aataggaaac
    17161 tgacgcagta ttcaacgaat acaaggagtt ttatcttttt cacgcagcat cccgttccag
    17221 ctcacatcgg ctaactaact ttagcctggg ttcaaattag ctaaatcgat tagtattagc
    17281 tataactcag cttaccatct cgtaagttga aaccaacaat agcatgaaaa cattgagaac
    17341 gggtaggtcc tgcctatccg tttttattaa aatcgtgtta taatagaata gaaatcaaaa
    17401 ataaatagga gaaacaaacc tcatggcacg cgaattttca cttgaaaaaa ctcgtaatat
    17461 cggtatcatg gctcacgtcg atgccggtaa aacaacaact actgagcgta ttctttacta
    17521 cactggtaaa atccacaaaa tcggtgaaac tcacgaaggt gcgtcacaaa tggactggat
    17581 ggagcaagag caagaacgtg gtatcacgat cacatctgct gcgacaacag ctcaatggaa
    17641 caaccaccgc gtaaacatca tcgacacacc aggacacgtg gacttcacaa tcgaagtaca
    17701 acgttctctt cgtgtattgg atggtgcggt taccgttctt gactcacaat caggtgttga
    17761 acctcaaact gaaacagttt ggcgtcaagc aactgagtac ggagttccac gtatcgtatt
    17821 tgccaacaaa atggacaaaa tcggtgctga cttcctttac tctgtaagca cacttcacga
    17881 tcgtcttcaa gcaaatgcac acccaatcca attgccaatc ggttctgaag atgacttccg
    17941 tggtatcatt gacttgatca agatgaaagc tgaaatctat actaacgacc ttggtacgga
    18001 tatccttgaa gaagacatcc cagctgaata ccttgaccaa gctcaagaat accgtgaaaa
    18061 attgattgaa gcagttgctg aaactgacga agaattgatg atgaaatacc tcgaaggtga
    18121 agaaatcact aacgaagaat tgaaagctgg tatccgtaaa gcgactatca acgttgaatt
    18181 cttcccagta ttgtgtggtt cagccttcaa aaacaaaggt gttcaattga tgcttgatgc
    18241 ggttatcgac taccttccaa gtccacttga catcccagca atcaaaggta ttaacccaga
    18301 tacagacgct gaagaaactc gtccagcatc tgacgaagag ccatttgcag ctcttgcctt
    18361 caagatcatg actgacccat tcgtaggtcg tttgacattc ttccgtgttt actcaggtgt
    18421 tcttcaatca ggttcatacg tattgaatac ttctaaaggt aaacgtgaac gtatcggacg
    18481 tatccttcaa atgcacgcta acagccgtca agaaatcgac actgtttact caggtgatat
    18541 cgctgctgcc gttggtttga aagatactac aactggtgac tcattgacag atgaaaaagc
    18601 taaaatcatc cttgagtcaa tcaacgttcc agaaccagtt atccaattga tggttgagcc
    18661 aaaatctaaa gctgaccaag acaagatggg tatcgccctt caaaaattgg ctgaagaaga
    18721 tccaacattc cgcgttgaaa caaacgttga aactggtgaa acagttatct caggtatggg
    18781 tgaacttcac cttgacgtcc ttgttgatcg tatgcgtcgt gagttcaaag ttgaagcgaa
    18841 cgtaggtgct cctcaagtat cttaccgtga aacattccgc gcttctactc aagcacgtgg
    18901 attcttcaaa cgtcagtctg gtggtaaagg tcaattcggt gatgtgtgga ttgaatttac
    18961 tccaaacgaa gaaggtaaag gattcgaatt cgaaaacgca atcgtcggtg gtgtggttcc
    19021 tcgtgaattt atcccagcgg ttgaaaaagg tttggtagaa tctatggcta acggtgttct
    19081 tgcaggttac ccaatggttg acgttaaagc taagctttat gatggttcat atcacgatgt
    19141 cgactcatct gaaactgcct tcaagattgc ggcttcactt tcccttaaag aagctgctaa
    19201 atcagcacaa ccagctatcc ttgaaccaat gatgcttgta acaatcactg ttccagaaga
    19261 aaaccttggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgtag atggtatgga
    19321 agcacacggt aacagccaaa tcgttcgtgc ttacgttcca cttgctgaaa tgttcggtta
    19381 cgcaacagtt cttcgttctg catctcaagg acgtggtaca ttcatgatgg tatttgacca
    19441 ctacgaagat gtacctaagt cagtacaaga agaaattatt aagaaaaata aaggtgaaga
    19501 ctaatccgtc ctcactctag aaggaagtca cttagtggct tccttttgtc tttagaaaat
    19561 acctctaaat atggtaaaat agtagaagaa taatgtgagg aaaatgaatg tcaaatagtt
    19621 ttgaaatttt gatgaatcaa ttggggatgc ctgctgaaat gagacaggct cctgctttag
    19681 cacaggccaa tattgagcga gttgtggttc ataaaattag taaggtatgg gagtttcatt
    19741 tcgtattttc taatatttta ccgattgaaa tctttttaga attaaagaaa ggtttgagcg
    19801 aagaattttc taagacaggc aataaagctg tttttgaaat taaggctcgg tctcaagaat
    19861 tttcaaatca gctcttgcag tcctactata gggaggcttt ctctgaaggt ccatgtgcta
    19921 gtcaaggttt taagtccctt tatcaaaatt tgcaagttcg tgctgagggt aatcagctat
    19981 ttattgaagg atctgaagcg attgataagg aacattttaa gaagaatcat cttcctaatt
    20041 tagccaaaca acttgaaaag tttggttttc caacttttaa ctgtcaagtt gagaagaatg
    20101 atgtcctgac ccaagagcag gaagaggcct ttcatgctga aaatgagcag attgttcaag
    20161 ctgccaatga ggaagcgctc cgtgctatgg aacaactgga gcagatggca cctcctccag
    20221 cggaagagaa accagtcttt gattttcaag cgaaaaaagc tgcagctaaa cccaagctgg
    20281 ataaggcgga gattactcct atgatcgaag tgacgacaga ggaaaatcgt ctggtatttg
    20341 aaggggttgt ttttgatgtg gagcaaaaag tgactagaac aggtcgtgtt ttaatcaact
    20401 ttaaaatgac ggactatact tcaagttttt ctatgcaaaa gtgggttaaa aacgaggaag
    20461 aggcccagaa gtttgacctc atcaagaaga attcttggct ccgagttcga gggaatgtgg
    20521 agatgaataa cttcacacgc gatttgacta tgaacgtaca ggatctgcag gaagttgttc
    20581 actatgagcg gaaggatttg atgccagaag gtgagcgtcg ggttgagttt catgctcata
    20641 ctaacatgtc gactatggat gctttgccag aggtcgaaga gattgttgca acagctgcta
    20701 agtggggaca caaggcggtt gctatcacag accatgggaa tgtccagtcc tttccacatg
    20761 gctataaggc ggctaagaaa gcgggaatcc agctgatcta tgggatagaa gccaatatcg
    20821 tggaggaccg tgtccctatc gtctataacg aagtggagat ggacttatca gaagcaacct
    20881 acgtggtctt tgacgtggaa acgacgggac tttcagctat ctataatgac ttgattcagg
    20941 ttgcggcctc taagatgtac aaggggaatg ttattgctga atttgatgaa tttatcaatc
    21001 ctgggcatcc cttgtcagct tttactacag agttaactgg aattacagat gatcatgtca
    21061 aaaatgccaa accactagaa caagttttgc aagaattcca agaattttgc aaggatacgg
    21121 tcctagttgc ccacaatgct gcctttgacg ttggctttat gaatgctaat tatgagcgtc
    21181 atggtcttcc aaagattagt cagccagtta ttgatacgct ggagtttgct agaaacctct
    21241 atcctgagta taaacgccat ggtttggggc ctttgaccaa gcgttttggt gtggccttgg
    21301 aacatcacca catggccaac tacgatgcgg aagcgactgg tcgtctgctt ttcatcttta
    21361 tcaaagaggt agcagaaaaa catggtgtga ccgatttagc tagactcaac attgatctaa
    21421 tcagtccaga ttcttacaaa aaagctcgga tcaagcatgc gaccatctat gtcaagaatc
    21481 aggtaggtct aaaaaatatc tttaagctgg tttccttgtc taataccaag tattttgaag
    21541 gagtgccacg gattccgaga acggttctag atgcccatcg agagggcttg attttaggtt
    21601 cagcctgttc agagggtgaa gtttttgacg tggtcgtttc tcaaggtgtg gatgcggcgg
    21661 ttgaggtggc caagtattat gactttatcg aggtcatgcc accggctatc tatgcgccct
    21721 tgattgctaa agagcaggtc aaggatatgg aggaactcca gaccattatc aagagtttga
    21781 tagaggttgg agaccgcctt ggcaagcctg ttttggctac gggaaatgtt cactatatcg
    21841 aaccggaaga agagatttat cgtgaaatta tcgtccgtag tttgggacag ggtgcgatga
    21901 ttaaccgaac tatcggtcat ggtgaacatg cccaaccagc accacttcca aaggctcatt
    21961 ttcgaacgac taatgagatg ttggatgaat ttgccttttt gggagaggaa ctggctcgta
    22021 aactggttat tgaaaacacc aatgccttgg cagaaatatt tgaacccgtt gaagtcgtta
    22081 agggtgactt gtatacgcct ttcatcgaca aggctgaaga aacagttgct gagttgacct
    22141 ataagaaagc ttttgagatt tatggaaatc cgctgccaga tattgttgat ttgcggattg
    22201 aaaaagaatt aacatccata ctggggaatg gatttgctgt gatttatctg gcatcgcaga
    22261 tgctggtgca acgttctaat gaacggggtt atttggttgg ttctcgtggg tctgtcggat
    22321 ctagtttcgt tgcgaccatg attggaatta cggaggtcaa tcctctctct cctcactatg
    22381 tctgtggtca gtgtcagtac agtgagttta tcacagatgg ttcgtacggt tcaggatttg
    22441 atatgcccca taaggactgt ccaaactgtg gtcacaaact cagtaaaaac ggacaggata
    22501 ttccgtttga gaccttcctt ggttttgatg gggataaggt tcctgatatt gacttgaact
    22561 tctcgggaga agatcagcct agcgcccact tggatgtgcg tgatatcttt ggtgaagaat
    22621 atgccttccg tgcgggaaca gttggtacgg tagctgccaa gactgcctat ggatttgtca
    22681 agggttacga gcgagattat ggcaagtttt atcgtgatgc agaagtagaa cgcctcgctc
    22741 aaggagcggc gggtgtcaag cggacaacag gccaacaccc ggggggaatc gttgttattc
    22801 cgaactacat ggatgtctac gattttacgc ctgtccagta tccagcagat gatgtcacgg
    22861 ctgaatggca gaccactcac tttaacttcc acgatatcga tgagaacgtc ctcaaactcg
    22921 atgtactggg acatgatgat ccgaccatga ttcgaaaact tcaggatttg tctggtattg
    22981 accctaataa aattcctatg gatgacgaag gcgtgatggc actcttttct gggactgatg
    23041 tgctaggggt aacacctgaa caaattggaa cgcctacggg tatgttgggg attccagagt
    23101 ttggaacaaa tttcgtacgt ggaatggtag acgaaaccca tccgacaacc tttgcggaat
    23161 tgcttcagct gtctggtctg tcccacggta ctgatgtttg gttggggaat gctcaggatc
    23221 tgattaagca aggaatagcg gacctatcga ctgttatcgg ttgtcgggac gacatcatgg
    23281 tttacctcat gcatgcgggt ctggaaccta agatggcctt taccattatg gaacgggtac
    23341 gtaagggttt gtggctaaag atttcagaag aggagagaaa tggctatatc gaagcaatga
    23401 aggctaataa ggtgccagag tggtatatcg aatcctgtgg gaaaattaag tacatgttcc
    23461 ctaaggccca tgcggcagcc tacgttatga tggccttgcg tgtagcttac ttcaaggttc
    23521 accatcctat ttattactac tgtgcttact tctccattcg tgctaaggct tttgatatca
    23581 agaccatggg tgcgggcttg gaggtcatca agcgcagaat ggaagaaatc tctgaaaaac
    23641 ggaagaacaa tgaagcctct aatgtggaaa tcgatctcta tacaactctt gagattgtca
    23701 atgagatgtg ggaacgaggt ttcaagtttg gtaaattaga tctctaccgt agtcaggcga
    23761 cagagttcct catcgacggg gataccctta tcccaccatt tgtagcaatg gatggtctgg
    23821 gagagaacgt tgccaagcaa ctggtgcggg cgcgtgaaga gggagaattc ctctctaaaa
    23881 cagaactacg caagcgtggt ggactctcat caaccttggt tgaaaagatg gatgagatgg
    23941 gtattcttgg aaatatgcca gaggataacc agttgagttt gtttgatgag ttgttttaaa
    24001 aaattgctta ataatctatt aaaagaggct aacgtatatc caatagattt acattagctt
    24061 tcttttttgt taaaatagtc tatggaaaga gggtgagagt atgtcaaaga tgagtataag
    24121 catccgtctg gatagtgagg ttaaggagca ggcccaacag gtgtttagta atctgggaat
    24181 ggatatgaca acagctatta atattttcct tcgtcaggca attcaatatc agggattacc
    24241 ttttgatgtt agactagacg aaaatcggaa gttgctccaa gcgttaacgg atttagacca
    24301 aaatcgtaat atgagccagt cttttgaatc agtctcagat ttgatggagg acttacgtgc
    24361 ttaagattcg ttatcataaa cagtttaaaa aagattttaa gttggctatg aagcgtggtt
    24421 tgaaggcaga attattagaa gaagttttga attttctggt tcaagaaaaa gaacatcctg
    24481 ccagatatcg tgatcattca ttgacggcat ccaagcattt tcaaggagtt cgtgaatgcc
    24541 atacccagcc agattggctt ttggtttata aagtagacaa gtcggaattg attttaaatt
    24601 tgctgaggac aggcagtcac agtgatttat tttaatctat tttaaggggg ttctcatgaa
    24661 actaagaata tttgcggaag ataagccggc taagaaggta tttgaatatc aattagaact
    24721 tgctgatcgt acaattcttc tatcgacagc actcttgtca ggtgctattg ctttagcagg
    24781 aatcttttct gctttgaaag aaaaataaaa atagaaaaga gaaaacagaa tggttttacc
    24841 aaattttaaa gaaaatctag aaaaatatgc gaaattgttg gttgcgaacg gaattaacgt
    24901 gcaacctggt cacactttgg ctctctctat tgatgtggag caacgtgaat tggcacatct
    24961 aatcgtgaaa gaagcttatg ccttgggtgc gcatgaggtc atcgttcagt ggacagatga
    25021 tgtgattaac cgtgagaaat tcctccatgc cccgatggag cgtttggaca atgtgccaga
    25081 atacaagatt gctgagatga actatctttt ggagaacaag gctagccgtc ttggggttcg
    25141 ttcatctgat ccaggtgcct tgaacggagt ggacgctgac aagctttcag cttctgctaa
    25201 agctatggga cttgccatga agcctatgcg tatcgcaact caatctaaca aggttagctg
    25261 gactgtagca gccgctgctg gacttgagtg ggctaagaaa gtcttcccaa atgctgcgag
    25321 cgacgaagaa gcagttgatt tcctttggga tcaaatcttc aaaacttgtc gtgtctacga
    25381 agcagatcct gttaaggctt gggaggaaca tgcagccatt ctcaagagca aggccgatat
    25441 gcttaataag gagcaatttt cagcccttca ctacacagcg ccaggaacag atttaacact
    25501 tggtttgcca aagaaccacg tttgggaatc agctggtgct gtcaatgcac agggcgaaga
    25561 attcttgcca aatatgccga cagaagaggt cttcacagcg cctgacttcc gtcgtgcaga
    25621 tggttatgtc acttctacaa aaccgcttag ctacaacgga aatatcattg aaggcattaa
    25681 ggtgaccttt aaggatggac aaatcgtaga tatcactgct gagaagggtg atcaggttat
    25741 gaaagacctt gtctttgaaa atgcgggtgc gcgtgccttg ggtgaatgtg ccttggtacc
    25801 agatccaagt ccaatttctc agtcaggcat tacctttttt aacacccttt tcgatgaaaa
    25861 tgcgtcaaac cacttggcta tcggtgcagc ctatgcgact agcgttgttg atggagcgga
    25921 gatgagcgaa gaggagcttg aagctgcagg gcttaaccgt tcagatgttc acgtagactt
    25981 tatgattggt tctaaccaaa tggatatcga tggtattcgt gaggatggaa cgcgggtacc
    26041 tcttttccgt aatgggaatt gggcaaatta aggagataat atgttaggaa gtatgttcgt
    26101 tggtctccta gtgggatttt tagcaggtgc tatgaccaat cgtggagagc gaatgggatg
    26161 ttttggaaaa atgtttctcg gttggatcgg agcctttcta ggtcacttgc tctttggaac
    26221 ttgggggcca gttttatcag gaacagctat tatcccagcg attttaggag ccatgattgt
    26281 tttagctatt ttttggagac gaggaagtta atttcctaaa tctgatactc aatgaaaatc
    26341 aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga ggttgcagat
    26401 agaactgacg aagtcagcta aaaacactgc tttgaggttg cagatagaac tgacgaagtc
    26461 agctaaaaac actgttttga ggttgcagat agaactgacg aagtcagcta aaaacactgt
    26521 tttgaggttg cagatagaac tgacgaagtc agtaacatat atacggcaag gcgacgttga
    26581 cgcggtttga agagattttt gaagagtatg aaacgaaaag gaggttggtc attgtactag
    26641 cctccttttg atatgatata atagttctat gagattagat aaatttttag ttgcctgtgc
    26701 ggtgggaagt cggactgagg tcaaaaactt gctcaaggct gggcgcgtga ctgtaaatgg
    26761 taaaaaagaa aaatcagcta aattgcagat tgatgaaaaa atagatgaga ttcgctttga
    26821 tgggcaagtg ttggagtatg aagagtttgt ctactacatg atgaacaagc ccaaaggagt
    26881 tatctcagcg actgaggatc ccaagcacag aaccgttctg gacttgttgg atgacttggc
    26941 gcggagtaag gaagtttttc cagtaggacg cttggatatt gacacgcatg gtcttttgct
    27001 cttgaccaat aatggtcagc tggctcatgt tcttctttcg cccaagcgtc atgtggacaa
    27061 gacttatcag gctcaggtca agggaattat gacccaagaa gatgtggaga catttatcaa
    27121 gggcattccg ctcaaagact ttacctgtca gcccgctaga ctggagattc tttccacaga
    27181 tgcagaaaag aatcagagcc aaatccgtgt gaccattgca gaagggaagt ttcatcagat
    27241 caagcgtatg gtgggctact gtggcaagga agtggtggac ttgcaacgat tgactatggg
    27301 aactttagta ttagatgaga acctggaacg aggggaatgg cgtcgcttga ccaaggaaga
    27361 attagaaatt ctccgcgcta atattattta atttagttta atactcaatg aaaatcaaag
    27421 agcaaactag gaaactagcc gcaggctgta cttgagtacg gcaaggcgac gttgacgtgg
    27481 tttgaagaga ttttcgaaga gtataaattc gaaaaaggtg agggagaatg tccttacctt
    27541 ttatgttttt tagttgcttc ctttgtgaaa agagttataa tagactgtag aataaaagga
    27601 ggaatctatg aacgcgattc aagaatcatt tactgataaa ctatttgcca actatgaagc
    27661 aaatgtaaaa taccaagcga ttgaaaatgc tgccagccac aacggaattt ttgcagctct
    27721 ggaacgtcgc caaagccatg tagacaacac acctgttttc tcattggatt taaccaagga
    27781 caaggtcact aaccagaaag cgtctggtcg ttgctggatg tttgcggctc tcaacacctt
    27841 ccgccacaaa ctcatctcgc aatacaaatt ggagaacttt gagttgtcac aagcccacac
    27901 tttcttctgg gataagtatg agaaatcaaa ctggttcttg gagcaagtca ttgcgacttc
    27961 agaccaagaa ttgactagcc gcaaggttag cttcttactc caaacacctc aacaagatgg
    28021 tggtcaatgg gatatggtcg tttccctctt tgaaaaatac ggtgtcgtgc ctaagtcagt
    28081 ttatcctgag tctgtttcat ctagcagcag tcgtgagcta aatgcgatcc ttaataaatt
    28141 gcttcgtcaa gatgctcaaa tcttgcgtga tttgcttgtt tctggtgcag atcaagtgac
    28201 tgttcaagct aagaaagaag acctcttgca agaaatcttt aactttcttg ctatgtcatt
    28261 aggacttcca ccacgcaagt ttgactttgc ttatcgcgat aaagataaca actacaaaag
    28321 tgaaaaagga atcacaccac aagagttcta caagaaatat gtcaatcttc ctttagaaga
    28381 ctacgtttct gttatcaatg ctccaactgc tgataaacct tacggaaaat cttacacagt
    28441 tgagatgttg gggaatgtgg ttggtagccg tgcagttcgt tacatcaacg ttccaatgga
    28501 gcgcttgaaa gaattggcga ttgcccaaat gcaagcaggt gagactgttt ggtttggttc
    28561 tgatgtcggc cagctcagta accgtaaggc tggaatcctt gcgacagatg tttatgactt
    28621 tgaatcaagc atggacatta aacttactca agacaaggct ggacgtttgg actatagcga
    28681 aagcttgatg acccacgcca tggtcttgac aggtgttgac ttggacgaaa atggtaaatc
    28741 aaccaagtgg aaggttgaaa actcatgggg agacaaggtc ggtacagatg gttactttgt
    28801 tgcctcagac gcttggatgg acgaatacac ataccaaatc gttgttcgta aggaattgct
    28861 gacagcagaa gaacaagctg cctatggagc agaaccaatt gtacttgcac catgggatcc
    28921 aatgggagcc ttggctaaat aaaagcatag aaaaaaggaa tcagatttta gaacctggtt
    28981 cctttttagt tgcttgatta catgatgtga agaacatgtg ccacaatacc cactgcgaag
    29041 agggcaagga taatagtgat tggagatact tttttcttaa gtaagtacat gcaaagtaaa
    29101 gtaaggagta gtcctgataa tccaggaatc aacatatcca agttttgttg gaaagtagta
    29161 actttttcag gagtttgaga caatccttgt cctacttgtg cgaatgcttc ttggatacct
    29221 ttagaccctt ctggcaattt atcccaatgg atataagcct tttcatctag ttgaacttta
    29281 gaaacatcga aagcaaattt aatatttacc cagcgttgaa caaggacagc aagaatgaac
    29341 atcccaagga tagaagctcc tttagtgata tcttgaagga taccaccaga catatcttta
    29401 gtgatttctg atccagcctt gtatccaatc tcttgaacat accacaagaa tgacatacga
    29461 atcaagttcc atgcaacaaa gaagaggagt ggccccaaga tattgccagt aagggcaagt
    29521 gaagcaccga gagatccaag gattgggcgt actgtaaacc agaatactgg gtcaccgata
    29581 ccagcaagag gtcccatcat accgatttta accccttgga tagcagcgtc atcgatttcc
    29641 acaccgttag cacgttcttc ttcaagcgca agagtaaccc ccatgactgg agcagctacg
    29701 tatggatgag tgttgaagaa ctcaaggtga cgctcaagag cagcgatttg atcttctttt
    29761 ttagtataga gttttttgat agctggaatg agtgtataag cccagcccaa gttttgcatc
    29821 cgttcaaagt tccaagaccc ttgtaagaag gttgaacgcc accaaacttt tttacgatct
    29881 gatttagtta attgaagttt ttcagtcatg atgttttcag tcctttctta tcttagtagt
    29941 cttctaggat atcgccgatt gggtcgttag aagttgcggc tcctccgcca ccatttccac
    30001 cagttttaga aaggtgaagg tagataagag cgatagcaac accgatagca ccgaatccga
    30061 ttagagtaat atctgacaca gcagcgagaa cgaaaccaag agcgaagaat ggccatactt
    30121 cacgagttgc catcatgttg ataaccatgg cgtaaccaac ggcaacgacc ataccaccac
    30181 cgatagccat accatctttg agccagtctg gcatggcact aaggatactt tgtacagttt
    30241 cagttggtac cataaggaga agagctgcag gaagcgcgat acgaagtcct tggaagagta
    30301 gcgcaatgaa atgcgcacgc tccacagcgc cgaagtcacc ttttttagcg gcagcatctg
    30361 cagtatgaac caaaccaact gaaattgtac gaacaatcat tgtcaagaaa agtccagcta
    30421 cggcaagagg gatagcaacc gcttgggcaa caccgatacc agtcttggta aagtcaccac
    30481 caagaaccat gataatggca gcagcgacag aagcaagtgc agcatcagga gcgatagcag
    30541 caccgatatt tgaccaacca agggcaatca tttgaagcga tccaccgagg ataatccctg
    30601 cttccaagtg acctgttaca agcccaataa gggtacaggc tacaagtggt tggtgaaatt
    30661 ggaactggtc gaggatgcct tcaagacctg caaagaaggc tacaacgact actaaaacca
    30721 tagaaataat agacatgttt aaaatccttt cataaataat ggcttatttg acattggctt
    30781 tgttaatcaa gtcaaacaaa tcttttttag aatcatttgg tactttacgg acatcaaatt
    30841 caacacccaa gtcacgcatt ttttcaaatg tagcaacatc ttctttgtcc atagacaaaa
    30901 cggtattgac caatgtttta cctgttgagt gagccataga accaacgtta agagtcttga
    30961 ttggcacgcc gccttcgatg gcacgaaggg catcttgagg tgtttcaaac aagataaggg
    31021 catgtgtttc tccaaaacgt gggtcttttg aaatctcaat cagtttttga attggaacca
    31081 cgttagcctt gacattacct ggagctgctt gtttaatcaa ttctttacga aggtcgtctt
    31141 tagccacgtt atctgaagca acgatgatac gatttgcttt tgaatctgga gtccaagcag
    31201 ttgcaacctg accgtgaagt agacgtgtgt caagacgggc aagattgatt ttcaatttac
    31261 cgtctccgat aacagttcct tctgggatag cagtttgggc aactggagca gctgcagcgc
    31321 ttgcaacttc ttcaactgga tttagctctt ctggaagagc tttgatgcca tctttggctt
    31381 ctttaatgat attagcagcg actttttcta cacctgcagc agcgtccatg aggcgctctg
    31441 tgtaggcttg aatcaacatc ggtaagttaa gtcctgtgat gatggcaaac ttacgctcag
    31501 gattttctcc catcacgcga ctagcttggt taaatggaga accactccaa aggtcagcca
    31561 aaactagaac ctcatcttct gcgtcaaatg cagcaacagc gttattaaac ttagcgtata
    31621 gatcatcagg accttcattt ggcataaagg tcacaacttg aaccttttct tgttcaccaa
    31681 agatcataga tcctgactga tgaatacccg cagcaaattc gccgtggctc gcaataatga
    31741 ttccgatact cattattgtc attcctcctt ttttgtttta gttttcattt aagaaaactt
    31801 taagactgtt taagtata
//
LOCUS       DAVLFB010000016        27642 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLFB010000016 DAVLFB010000000
VERSION     DAVLFB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.016"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..382
                     /locus_tag="VXD58_000629"
     CDS             <1..382
                     /locus_tag="VXD58_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675374.1"
                     /translation="FICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIF
                     MQICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLV
                     CIWIMGFEFFLLPKLIKLKEQNLV"
     gene            924..1145
                     /locus_tag="VXD58_000630"
     CDS             924..1145
                     /locus_tag="VXD58_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675375.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1662..1915
                     /locus_tag="VXD58_000631"
                     /pseudo
     CDS             1662..1915
                     /locus_tag="VXD58_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2043..3272
                     /locus_tag="VXD58_000632"
     CDS             2043..3272
                     /locus_tag="VXD58_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0675376.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3292..5988
                     /gene="ppc"
                     /locus_tag="VXD58_000633"
     CDS             3292..5988
                     /gene="ppc"
                     /locus_tag="VXD58_000633"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV0675377.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6071..6288
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6401..7405
                     /locus_tag="VXD58_000634"
     CDS             6401..7405
                     /locus_tag="VXD58_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0675378.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7474..8340
                     /locus_tag="VXD58_000635"
     CDS             7474..8340
                     /locus_tag="VXD58_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675379.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8337..9095
                     /locus_tag="VXD58_000636"
     CDS             8337..9095
                     /locus_tag="VXD58_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675380.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9185..10945
                     /gene="dnaG"
                     /locus_tag="VXD58_000637"
     CDS             9185..10945
                     /gene="dnaG"
                     /locus_tag="VXD58_000637"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV0675381.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KLASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10948..12057
                     /gene="rpoD"
                     /locus_tag="VXD58_000638"
     CDS             10948..12057
                     /gene="rpoD"
                     /locus_tag="VXD58_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV0675382.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12071..12400
                     /locus_tag="VXD58_000639"
     CDS             12071..12400
                     /locus_tag="VXD58_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV0675383.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12515..13558
                     /gene="cpoA"
                     /locus_tag="VXD58_000640"
     CDS             12515..13558
                     /gene="cpoA"
                     /locus_tag="VXD58_000640"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV0675384.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13574..14899
                     /locus_tag="VXD58_000641"
     CDS             13574..14899
                     /locus_tag="VXD58_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0675385.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14930..15106
                     /locus_tag="VXD58_000642"
     CDS             14930..15106
                     /locus_tag="VXD58_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0675386.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15165..15299
                     /locus_tag="VXD58_000643"
     CDS             15165..15299
                     /locus_tag="VXD58_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV0675387.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15363..16673
                     /gene="obgE"
                     /locus_tag="VXD58_000644"
     CDS             15363..16673
                     /gene="obgE"
                     /locus_tag="VXD58_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV0675388.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16683..16841
                     /locus_tag="VXD58_000645"
     CDS             16683..16841
                     /locus_tag="VXD58_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675389.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17928..19187
                     /locus_tag="VXD58_000646"
     CDS             17928..19187
                     /locus_tag="VXD58_000646"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV0675390.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19203..19754
                     /locus_tag="VXD58_000647"
     CDS             19203..19754
                     /locus_tag="VXD58_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675391.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19747..21024
                     /locus_tag="VXD58_000648"
     CDS             19747..21024
                     /locus_tag="VXD58_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0675392.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            21040..21900
                     /locus_tag="VXD58_000649"
     CDS             21040..21900
                     /locus_tag="VXD58_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV0675393.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            22281..23083
                     /locus_tag="VXD58_000650"
                     /pseudo
     CDS             22281..23083
                     /locus_tag="VXD58_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            23274..25565
                     /gene="pcrA"
                     /locus_tag="VXD58_000651"
     CDS             23274..25565
                     /gene="pcrA"
                     /locus_tag="VXD58_000651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV0675394.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            25608..26288
                     /gene="radC"
                     /locus_tag="VXD58_000652"
     CDS             25608..26288
                     /gene="radC"
                     /locus_tag="VXD58_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV0675395.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(26285..26974)
                     /locus_tag="VXD58_000653"
     CDS             complement(26285..26974)
                     /locus_tag="VXD58_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0675396.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(26992..27633)
                     /locus_tag="VXD58_000654"
     CDS             complement(26992..27633)
                     /locus_tag="VXD58_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV0675397.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
BASE COUNT         8430 a         4857 c         6092 g         8263 t
ORIGIN      
        1 ttttatttgt tattttgttt atacaatttt ttctttaatt caattaggtg gtatgggaac
       61 tgatatgtta ttaaccaata taatatttcc caatataatg tcatttcttt tatttttacc
      121 tatagctatc tttatgcaaa tttgctgtat aagatttgat tccattttag caaaggcctt
      181 acctaatatt ttgctgatac ttattgtttt aatcacgttt cagagtgatt ggaatatatt
      241 tatacctgca acttattatt atacagaaat tcagtcaact acaaacttag gtattaaatt
      301 attagtttgt atctggatta tgggatttga atttttcctt cttccaaaac taataaaatt
      361 gaaagaacaa aatctagttt aaatgttgct cgaaaattaa ataattgaat acgattaaca
      421 atgaaacaat acaacgtgag tcatgacttc aaagtaattg attttatgat tctggtttct
      481 tacgcttaat taagcaagtt gtattcactt tatcaaattg ttattactct aaaaagcgtc
      541 taacagctat agagtgtctg tttgcttctc tttactttta ttttaaataa ttcattaacc
      601 ttccgtaagt gcccaataat aaggtgtgtt gaagaagtct gagataaaat agttctcagt
      661 cacaaaaaag ctatagattc ccattggtga acagctccct gtcaagtaga cagtaaaata
      721 atataaaatc ttaagcgacc tgattcctga attccagtgg agtcaggttg ttttttaacc
      781 gtctttctta atattgtctt acctataata gatatgctta tgagatatgt ttaataaatt
      841 aattttagtt caaaatgtcc agttaggaca ctttgaatga tttcttagtt ttatatatta
      901 tacttttctc aggagaaagc tagatgtaca gacgtttgag agatttgagg gaagatcacg
      961 atttgaccca aaaacaaata gctaaaatac tttcgtttac aaactcagct tatgctaaaa
     1021 ttgaacgggg tgagcatacg ttaacagcag atgtattggt aactctctca aacttttacg
     1081 atgtcagtac agactaccta ttggaattga cagattttcc tgataaaatt cgctttagaa
     1141 aataatctcc tcaatttcat agagtgtgaa aatgaggaga ttttttattt gttctttgaa
     1201 aattaaatag tccaaaatgg tactttcctc atttgtggag caaatttgaa tggctcgcca
     1261 tgataagagc gattttaaaa ccatcaataa aatagagcga tactttatat gccatgatac
     1321 aaatgatata caatgatact tctgaccgtt caggctgcca tcgtaaaaga gcagcaagtg
     1381 aaattcttat gatgacttca tcagtcatgc cacgttaaat gtgtgagttt gttagagaaa
     1441 cgcaattaat cctcaaaagg ttccccgaac cttttgagtt ccacagacgc atcacgtgga
     1501 gtgtgtaagc ttgttgctaa aagcgtaaaa accttggaac gaaaggaata atagacttcc
     1561 tgcgaaacaa aaatacaata caataaaact atggatgatg aagcaagtaa acaattaagc
     1621 gatagccgtt tcaagagcct tgtaggtgtt cagcgcacga gatgttagct gtgttaaaaa
     1681 cagcttatca acgcaacgta aacgcgcaaa aggtggacga aaaaccaaat taagcctaga
     1741 cgatctcctc atggtaacta ttcaatacat gcgagaatag cgcacttatg aacaaattgc
     1801 ggctgatttt ggcattcacg aaagcaactt aatccgtcgg agccaatggg ttgaagtaac
     1861 tcttgttcaa aatggtttta cgatttcaaa ttctgcccta attatgaaaa acagtaaaat
     1921 tcgaaggatt gtaaggtaag agtttttttc tttctgaaaa aatggtataa tagcaatcaa
     1981 aactagaaaa taaaacggaa tttggaacag atttgtctgt atcctagtag agtggtgatg
     2041 ctatgaagat tagtaagagg cacttattaa attattccat cttgattccc tacttgcttt
     2101 tatctatttt gggcttgatt gtggtctatt cgaccaccag tgctatttta attgaagaag
     2161 gcaagagcgc cttgcagttg gttcgaaacc aaggaatctt ttggattgtt agtttgatac
     2221 tgattgcctt aatttataaa ttgagactag attttttgag aaatgagcga ctaatcattt
     2281 tagttatatt aatagaaatg cttttattgt tcttggctcg ttttattggt atttccgtaa
     2341 acggggcata cggttggatt tcggttgcag gagtaactat tcagccagct gagtacttaa
     2401 aaatcattat tatttggtat ttagctcacc gattctccaa acagcaagaa gaaatagcta
     2461 cttatgattt tcaagttttg actcaaaatc aatggcttcc ccgtgctttt aatgattggc
     2521 gattcgttct cctagttctg attggaagtt tgggaatttt ccctgattta ggaaatgcga
     2581 ctattttagt cttggtttcc ttgattatgt atacagttag tggaatcgct tatcgctggt
     2641 tttcaaccat tctggcgctc gtatctgccg cttctgtctt tgtcttgacc actatcagcc
     2701 taatcggtgt tgagaccttt tcaaaaattc cagtatttgg ctatgtagcc aagcgcttta
     2761 gtgccttttt taatcctttt gccgatcgtg ctgatgcagg tcaccagtta gctaattctt
     2821 attttgccat ggtcaatggt ggttggtttg gtctaggtct tggaaactcg attgaaaaac
     2881 gaggttattt gccagaagct catacagact ttgtcttttc tatcgtgatt gaagaatttg
     2941 gctttgctgg tgccagtctt attttagctc tcttgttttt catgattttg cggattatct
     3001 tggtcggtat tcgagcggag aatcctttca atgccatggt tgcactcggt gtcggaggga
     3061 tgatgttggt tcaggtattt gtcaatatcg gagggatttc gggcttgatt ccatctacag
     3121 gagtaacctt ccccttctta tcccagggtg gaaatagtct tctagtctta tcagtggcag
     3181 tagcctttgt cttaaatatt gatgccagtg aaaaacgcgc taagttgtac cgagaattgg
     3241 aaaatcaacc aatgaacctt ctgttgaagt aggataaaga aaggatagtt tatgtctctt
     3301 caaaaattag aaaattatag taataaaagt gttgtgcaag aagaagtctt gattctaaca
     3361 gaattactgg aagatattac taaaaatatg cttgccccag agacctttga aaaaataata
     3421 cagttgaaag aattatcaac gcaggaagat tatcaaggtc taaaccgtct agtgactagc
     3481 ttatcaaatg atgaaatggt ctatatttca cgctatttct ctatcttacc tcttttgatc
     3541 aatatttcag aggatgtgga tttagcttat gaaatcaatc atcaaaataa tattgatcag
     3601 gactatttag gtaaattatc tacaacgatt aaattggtag cagaaaagga aaatgccgtt
     3661 gagatcctag aacacttgaa tgttgtccct gttttgacag cccatccaac acaagtgcaa
     3721 cgcaaaagta tgttggattt aacaaatcat attcatagtc ttttgcgtaa ataccgtgat
     3781 gttaagttgg ggttgatcaa taaagataaa tggtacaatg atttgcgtcg ttacatcgaa
     3841 attatcatgc agacagacat gattcgtgag aaaaaattaa aagtgactaa cgaaatcacg
     3901 aatgctatgg aatattataa cagctccttt ttgaaagctg tacctcattt gacgacggag
     3961 tataagcgct tagcgcaagc gcatggtctg aatttaaaac aggctaaacc aatcaccatg
     4021 ggtatgtgga taggtggtga ccgtgatgga aatccatttg ttacagcaga gaccttgaag
     4081 cagtctgcac tcactcaatg tgaagtcatc atgaactact atgataaaaa gatttaccaa
     4141 ctttatcgtg aattttctct ttcaactagc attgtcaacg tcagcaagca agtcagagaa
     4201 atggctcgtc aatccaagga taactcgatt taccgcgaaa aagagcttta ccgtcgtgcc
     4261 ttgtttgata ttcaatcaaa aattcaggca actaaaacct atctgattga ggatgaagaa
     4321 gttgggactc gttatgaaac cgccaatgat ttctacaagg atttgattgc cattcgagat
     4381 tctctactag aaaataaggg tgagtccttg atttcaggtg attttgtgga attattgcag
     4441 gcagtagaga tatttggttt ttacctagca tcaattgata tgcgacaaga ctctagcgtc
     4501 tatgaagcct gtgtggcaga actcttgaaa tcagcaggaa ttcattctcg ttatagcgag
     4561 ttgagcgaag aagaaaagtg tgaccttctc ttgaaagaat tagaagaaga tccccgaatt
     4621 ctttctgcga ctcacgcaga aaaatcagaa ttattagcaa aagaattagc tatttttaag
     4681 acggctcgtg ttttgaaaga taagttggga gatgatgtca tccgtcagac catcatttca
     4741 catgcaacca gcctttctga tatgctagaa ttagctattc tgttaaaaga agtaggactg
     4801 gtggatacgg aaagggcgcg tgttcagatt gttccccttt ttgaaacaat tgaagacttg
     4861 gatcattcag aagaaacaat gagaaaatat ctttctctta gccttgccaa aaaatggatt
     4921 gactcacgaa ataactacca agaaatcatg cttggctact ctgacagtaa taaagatggc
     4981 ggttacttgt catcatgttg gaccctctac aaggctcaac aacaattgac tgctattgga
     5041 gatgaatttg gcgttaaggt taccttcttc catggtcgtg gtggtactgt cggtcgtggt
     5101 ggtgggccaa cctatgaagc cattacatct caaccgctca agtctatcaa ggatcgtatc
     5161 cgcttgacgg agcagggtga agtaattggg aataaatacg gtaacaaaga cgccgcttac
     5221 tataaccttg aaatgctagt atcggcagct attaaccgta tgattactca gaagaagagc
     5281 gataccaata ccccaaatcg ttatgaagcc attatggatc aagtagtgga ccgtagttac
     5341 gatatctacc gtgatttggt ctttggtaat gagcatttct atgattattt cttcgagtca
     5401 agtccaatca aggctatttc aagttttaat attggttctc gtccagccgc tcgtaagact
     5461 attactgaaa tcggtggttt gcgtgccatc ccttgggtat tctcatggtc acagagtcgt
     5521 gttatgttcc ctggatggta cggggttggt tcaagcttca aggaatttat caataaaaat
     5581 ccagagaata ttgctatctt acgagatatg taccaaaatt ggcctttctt ccaatcgctt
     5641 ctttcaaatg ttgatatggt tttgtcaaaa tcaaatatga atattgcttt tgaatatgct
     5701 aaactttgtg aagacgagca agttaaggcc atctatgaga ctattttaaa tgaatggcaa
     5761 gttactaaga acgttatctt ggctattgaa ggacatgacg aactcttagc tgacaatcca
     5821 tatctaaaag ctagtctgga ttaccgtatg ccttacttta atattctcaa ctatattcag
     5881 ttagagttga ttaaacgcca acgtcgtgga gaattgtcca gtgatcaaga acgattgatt
     5941 catatcacca tcaacggaat tgcgacagga ttgcgtaatt caggttgata attttcaaga
     6001 gtgaatgcta aaagtgaata tcaaaaaaat tctaatagac tattgacaag tagtttaaaa
     6061 atgatataat ttaaccattc agaaaagtaa tcatacaaac tttttagaga gtctgtggta
     6121 gctgaaaaca gataagtggc gatgatgaaa attgggctga atgctattta gaatttgaaa
     6181 ttataaaaat tcggtaagca caccttacag tgcatctcgt tattgcgaga ctgagcgata
     6241 gggaaattcc ctataattga ggtggtaccg cgcatcgacg tcctcacaca agttttttgt
     6301 gtgaggattt ttttgatgga ggttagtatg gaaagaaaac gatggcgtcg cttgtttaga
     6361 taagtgaaat atgttaaagg aaataaaaag gagaaacaga atgaaaaata aacgtttaat
     6421 tggaattatt gctgcattag cagtcttagt agcaggaagc ttgatttatt cttcaatgaa
     6481 taaatcagaa gctcagaata ataaggatga gaagaaaata actaagattg gtgtgcttca
     6541 atttgtgagc catccatccc ttgatttgat ttataaaggg atccaagatg gacttgcaga
     6601 agaaggatat aaagatgatc aagttaaaat tgattttatg aactcagaag gtgaccaaag
     6661 taaggttgcg acaatgagta aacaattggt tgcaaatggg aatgaccttg tggttggtat
     6721 cgcaacacca gcagcccaag ggttggctag tgcaacaaaa gacctaccgg ttatcatggc
     6781 cgctattaca gacccaattg gtgctaactt ggttaaagat ttgaaaaaac caggtggcaa
     6841 cattacaggg gtatctgacc acaatccagc tcaacaacaa gttgaactca tcaaggctct
     6901 gacaccgaat gtgaaaacaa tcggagctct ttactcaagt agcgaagaca attcaaaaac
     6961 acaggtcgaa gaatttaagg cttatgctga aaaagcaggt ctgacagtgg aaacatttgc
     7021 agttccttca acaaatgaaa ttgcctcaac tgtcactgtt atgactagca aggtagatgc
     7081 tatttgggtt ccaattgata acaccattgc atcaggattt ccaacggttg tctctagcaa
     7141 tcaaagttct aagaaaccaa tttatcccag tgcgacagct atggtagaag taggtggttt
     7201 ggcatcagtt gtaattgacc aacatgacct tggtgtggca acaggtaaaa tgattgtgca
     7261 agtcttgaaa ggtgcaaaac cagccgatac cccagtcaat gtcttttcaa ctggtaagtc
     7321 agtcatcaat aaaaaaatag cacaagaact aggtattact attcctgagt ctgttctcaa
     7381 agaagcagga caagtcatcg aataaataag gtagggagga ggagactctt cccatttttt
     7441 aaaaagatga atatagaaag gattaaataa aatatgattg tttccattat ttctcaagga
     7501 tttgtctggg ctattctagg tctgggaatc tttatgacat ttaggatttt aaactttcca
     7561 gatatgacga cagaaggttc cttccctctt gggggagctg ttgctgtcac tttgataacc
     7621 aaaggcgtga acccattttt agcgacactt gttgctgtag gagcaggttg tttggctgga
     7681 atggcagcag gccttcttta tacaaaaggg aagatcccaa ccttgctatc agggattttg
     7741 gtgatgactt cttgtcactc aatcatgctc ttgattatgg gacgtgcgaa tttaggcctg
     7801 cttggaacca agcaaattca ggatgttttg ccttttgatt cggatttgaa tcaactcttg
     7861 acaggtctca tctttgtgag tattgttatt gctctcatgc tctttttctt ggacactaaa
     7921 ctcggacaag cctatattgc tacaggggat aatcctgata tggctagaag tttcgggatt
     7981 catactggac gcatggagct catgggcttg gtcttatcaa atggtgtgat tgcccttgca
     8041 ggtgccctca ttgctcagca agaaggttat gccgatgtgt ctcgagggat cggggttatc
     8101 gttgtggggc ttgcaagttt gattattgga gaagttattt tcaagagttt gagcttggca
     8161 gagcgtttgg ttactatcgt tgtaggttct atcgcttatc aatttttagt gtgggcagtt
     8221 atcgcacttg gctttaatac aagttacctt cgtttataca gtgccttgat tttagcagtc
     8281 tgcctcatga ttccaacatt taagcaaaca atcttgaaag gagccaagtt aagcaaatga
     8341 cagcaattgt agaattaaaa aatgcaacca aaatcgttaa aaatggcttt gatgaagaaa
     8401 agattatttt aaatgatgtt tccttagaaa tttttgaacg ggactttatc acgattttgg
     8461 gcggaaatgg tgctggaaaa tcaaccctct ttaacactat agcagggacc ttatcactaa
     8521 ctagtggaac tatccgtatt ttaggtgaag aactcactaa gttttcaccc gagaagcgtg
     8581 ccaagtacct gtctcgtgtc ttccaagatc caaagatggg gacagctccc cgtatgacgg
     8641 tcgctgaaaa tcttttaatc gccaagtttc gtggtgaaaa gcgtggattg ttaccacgac
     8701 gcttgactag ctataaggat gaatttcagg caaccattga aaaagtagga aatggtcttg
     8761 agaaacactt gaatacaccg attgagttct tatcaggtgg acaaagacag gctttgagtc
     8821 tcttgatggc aaccttgaag cgacctgaat tactcctgtt agatgagcat actgctgccc
     8881 tggatccaaa gactagtgtt gctttgatgg aattgacaga tgaatttgtt aagaaagatc
     8941 agctaacagc ccttatgatt actcatcata tggaagatgc tctcaaatac ggcaatcgct
     9001 tgattgtcat gaaagaagga cggattatcc aagatttaaa ccaagaagaa aaagcaaaaa
     9061 tgaaaatctc tgattattat caactctttg aataaagtag ggaaaatgag tgaaatggtt
     9121 tacttttttt ctgaaataaa gtatactata taaagtaaac tatgataaca tggaggtatt
     9181 gtgtatggtt gacaaacaag tcattgaaga aatcaaaaac aatgccaaca ttgtggaagt
     9241 cataggagat gtgatttctt tacaaaaggc aggacggaac tatctagggc tctgtccttt
     9301 tcatggtgaa aaaacacctt ctttcaacgt tgtagaggat aagcagtttt atcactgttt
     9361 cggttgtggt cgctcaggtg atgtctttaa gttcatcgag gagtaccaag gggttccctt
     9421 tatagaggca gtccaaatct taggtcagcg tgttggaatt gaggttgaaa aaccgcttta
     9481 tagtgagcag aagctagcct cacctcacca agctctttat gatatgcacg aagatgcagc
     9541 caaattttac catgcgattc tcatgacaac gactatgggc gaagaggcca gaaattacct
     9601 ttatcagcgg ggtttgacag atgaagtgct taaacatttt tggattggtt tagcacctcc
     9661 agaacgaaac tatctctatc aacgtttgtc tgatcagtat cgtgaagagg atttactgga
     9721 ttcaggcctg ttttatctct cggatgccaa tcaatttgta gacacctttc acaatcgcat
     9781 tatgtttccc ctgacaaatg accaaggaaa ggtcattgcc ttctcaggtc gtatctggca
     9841 aaaaacggat tcacaaactt ctaagtataa aaacagccga tcgactgcaa tttttaacaa
     9901 aagttacgaa ttatatcata tggatagggc aaaaaaatct tctggaaaag ctagtgagat
     9961 ttacttgatg gaaggattca tggatgttat tgcagcctat cgggctggaa tcgaaagtgc
    10021 tgtggcgtcg atgggaacgg ccttgagtcg agagcatgtt gagcatctga aaaggttaac
    10081 caagaaattg gttcttgttt acgatggaga taaggctggg caagccgcga cattgaaagc
    10141 attggatgaa attggtgata tgcctgtgca aatcgtcagc atgcctgata acttggatcc
    10201 tgatgagtat ctacaaaaaa atggtccaga agacttggcc tatctattaa cgaaaactcg
    10261 tattagtccg attgagttct acattcatca gtacaaacct gaaaacagtg aaaatctgca
    10321 ggctcagatt gagtttcttg aaaaaatagc tcccttgatt gttcaagaaa agtccatcgc
    10381 tgctcaaaac agctatattc atattttagc tgacagtctg gcgtcctttg attataccca
    10441 gattgagcag attgttaatg agagtcgtca ggtgcaaagg cagaatcgca tggaaggaat
    10501 ttccagaccg acgccaatca ccatgccaat caccaagcag ttatcggcta ttatgagggc
    10561 agaagcccat ctactctatc ggatgatgga atcccctctt gttttgaacg attaccgttt
    10621 gcgagaagac tttgcatttg ctacacctga atttcaggtc ttatatgact tgcttggcca
    10681 gtatggaaat cttcctccag aagttttagc agagcagaca gaggaagttg aaagagcttg
    10741 gtaccaagtt ttagctcagg atttgcctgc tgaaatatcg ccgcaggaac ttagtgaagt
    10801 agagatgact cgaaacaagg ctctcttgaa tcaggacaat atgagaatca aaaagaaggt
    10861 gcaggaagct agccatgtag gagatacaga tacagcccta gaagaattgg aacgtttaat
    10921 ttcccaaaag agaagaatgg agtaataatg gcaacaaaac aaaaagaagt aacaacattt
    10981 gacgtacagg tagcagaatt tatccgtaat cataagcaaa aagggacagc aacagatgat
    11041 gaaatcaatg caagtctggt tattcctttt accttggacg ctgatgggat tgaagatctc
    11101 ttgcaacgga ttcaggatgc aggaatttct atcacagata acgaaggaaa tccaagtgcg
    11161 cgtgttctta gcaatgaaga agaaccagaa ctcagcgatg aggacttgat tgggtcaact
    11221 tctgctaagg tcaatgaccc tgtccgtatg tacttgaaag aaataggggt cgttcctctc
    11281 ttgaccaatg aagaggagaa agagttggca ctggctgttg aagctggtga tatcgaagcc
    11341 aaacaacgtc ttgcggaagc caatcttcgt ttggttgttt ccattgccaa acgctatgtc
    11401 ggtcgtggca tgcagttcct tgacttgatt caagaaggaa atatgggctt gatgaaggcg
    11461 gttgacaagt ttgactattc taaagggttc aagttttcaa cttatgcaac ttggtggatt
    11521 cgtcaggcta tcactcgtgc tattgcggac caagctcgta ccatccgtat cccagttcac
    11581 atggttgaaa ctatcaataa attggttcgt gaacagcgga atctccttca agaattgggg
    11641 caagatccga caccagaaca gattgctgaa cgaatggata tgacacctga taaggttcgt
    11701 gaaatcttga agattgccca agaaccagta tctcttgaaa ctcctatcgg tgaagaggac
    11761 gatagccacc ttggagactt tatcgaagat gaagtgattg aaaatccagt ggattatacg
    11821 actcgtatcg tcttgcgtga gcaattggat gaaatcttag atactcttac agaccgtgaa
    11881 gaaaatgttc tgcgtctacg ttttggacta gatgatggaa aaatgcgcac acttgaagat
    11941 gtggggaaag tctttaacgt aactcgtgag cgtatccgtc agattgaagc aaaggctttg
    12001 agaaaactac gccaaccaag tcgtagcaaa ccgcttcgtg attttattga agactaagag
    12061 tgaggaaaat atggcttata cagaagagca aattgaaaac atcaaaacac ggattttaac
    12121 agccttggaa gaagtcatcg accctgagtt gggaatcgat attgtcaatc ttggtttgat
    12181 ctatgagatt cgttttgacg gtgacacagg gcaaacagag attgacatga ctttgacaac
    12241 tatgggttgt cccttagcag accttttgac agaccagatt tatgatgcta tgatagaggt
    12301 tccagaagta acggatactg aggttaaatt agtctggtat ccagcctgga cagttgaaaa
    12361 aatgagtcgc tatgcccgca ttgccctagg cattaagtaa acatataaaa gcatgtgaga
    12421 gactgttgga aaagcgagga aatttcccct cttttcctct agtctctcct ttcttttgct
    12481 gattttattc aaagaaaatg atataatagt agttatggag aaaaagaaat tacgcatcaa
    12541 tatgttgagt tcaagtgaga aagtagcagg acagggagtt tcaggtgctt accgtgaatt
    12601 agttcgtctt cttcaccgtg ctgccaagga ccaattgatt gttacagaaa atcttccaat
    12661 cgaggcagat gtgactcact ttcatacgat tgattttccc tattatttat caaccttcca
    12721 aaagaaacgc tcagggagaa agattggcta tgtgcatttc ttgccagcta cacttgaggg
    12781 aagtttgaaa attccatttt tcttaaaggg aattgtgaaa cgctatgtat tttcttttta
    12841 caaccggatg gagcacttgg ttgtggtcaa tcctatgttt attgaggatt tggtagcagc
    12901 tggtattcca cgtgaaaaag tgacctatat tcctaacttt gtcaacaagg aaaaatggca
    12961 tcctctacca caagaagagg tagtcagact gcgcacagat cttggtctta gtgacaatca
    13021 gtttatcgta gtaggtgctg ggcaagttca gaaacgtaaa gggattgatg actttatccg
    13081 tctggctgag gaattgcctc agattacctt tatctgggct ggtggcttct cttttggtgg
    13141 tatgacagat ggttatgaac actataagaa aattatggaa aatcccccta aaaatttgat
    13201 ttttccaggc attgtatcgc cagagcggat gcgcgaattg tatgctctag cggatctttt
    13261 cttgttgcct agttacaatg agctctttcc tatgactatt ttagaagctg cgagttgtga
    13321 ggctcctatt atgttgcgtg atttagatct ctataaggta attttggagg gaaattatcg
    13381 ggcgacagcg ggtagagaag agatgaaaga ggctattttg gaatatcaag caaatcctgc
    13441 tgccttaaaa gatctcaaag aaaaggctaa gaatatttcc agagagtatt ctgaagagca
    13501 tctgttacaa atctggttgg acttttatga gaaacaagcc gctttaggga gaaagtaaaa
    13561 agtgaggtaa tctatgcgaa ttggtttatt tacagatacc tattttcctc aggtttctgg
    13621 tgttgcgacc agtattcgaa ccttgaaaac agaacttgaa aagcagggac atgctgtttt
    13681 tatctttacg acgacagata aggatgtcaa tcgctacgaa gattggcaaa ttatccgcat
    13741 tccaagtgtt cctttctttg cttttaagga tcgtcgcttt gcctaccgag gttttagcaa
    13801 ggcacttgaa attgctaaac agtatcagct agatattatc catactcaga cagaattttc
    13861 tcttggcctg ttggggattt ggattgcgcg tgaattgaaa attccagtca tccataccta
    13921 tcacacccag tatgaagact atgtccatta tattgctaag gggatgttga tccggccgag
    13981 tatggtcaag tatctggtta gaggtttcct gcatgatgtg gatggggtta tttgtcctag
    14041 tgagattgtc cgtgacttgc tatctgatta taaggtcaag gttgaaaaac gggtcattcc
    14101 tactgggatt gaattagcca agtttgagcg tccggaaatc aagcaggaaa atttgaaaga
    14161 actgcgtagt aaactaggga ttcaagatgg tgaaaagacg ttgcttagtc tttcgagaat
    14221 ctcctatgaa aaaaatattc aagcagtttt agcagccttt gctgatgttc tgaaagagga
    14281 agacaaggtt aaactggtag tagctgggga tggcccttat ctgaatgacc tcaaagagca
    14341 agctcagaac ctagagattc aagactcagt catctttaca gggatgattg ctcctagtga
    14401 gacggctctt tactataaag cggcggattt cttcatttcg gcatcgacaa gcgaaacgca
    14461 aggtttgacc tacttggaaa gcttagccag tggaacacct gtcattgctc acggaaatcc
    14521 ttatttgaac aacctcatca gtgataaaat gtttggaacc ttgtactatg gagaacatga
    14581 tttggctggt gctattttgg aagccctgat tgcaacacca gacatgaacg agcatacctt
    14641 atcagagaaa ttgtatgaga tttcagctga gaactttggg aaacgagtgc atgagtttta
    14701 tctggatgcc attatttcaa ataacttcca gaaagatttg gctaaagatg atacggtcag
    14761 tcagcgtatc tttaagacag ttttgtatct tcagcaacag gtggttgctg tacctgtaaa
    14821 aggatctaga cgcatgttga aggcttcaaa aacacagttg atcagtatga gagactattg
    14881 gaaagaccat gaagaataga aagaggaaca gctatgaaaa aaacaattaa tgagaagcgg
    14941 tcgtgataaa aagattgcgg gtgtttgtgc tggggtggcc cattatctgg atatggatcc
    15001 gactatcgtt caagtcattt ggggtgttct tacttgctgt tacggagctg gaattgtagc
    15061 ttacattatt ttatggatta tcgcaccagt agcaactgac tattgaaaac tagctcaatt
    15121 catgctaaga taatagaaaa tagaatgtaa cgaaggagag aaaaatggca tttggagata
    15181 atggaaatcg taaaaaaact atgtttgaga aaataacctt gtttatcgtg attatcatgc
    15241 tagtagcaag tttattggga atttttgcaa ctgcaattgg tgccctcagt aatctataaa
    15301 atagattcaa gaaaatttag tgactgggat ttcccagccc ttttttaaag tgagaagaaa
    15361 taatgagtat gtttttagat acagctaaga ttaaggtcaa ggctggtaat ggtggcgatg
    15421 gtatggttgc ctttcgtcgt gaaaaatatg tccctaatgg aggcccttgg ggtggtgatg
    15481 gtggtcgtgg aggcaatgtg gtcttcgttg tagacgaagg actacgtacc ttgatggatt
    15541 tccgctacaa tcgtcatttc aaggctgatt ctggtgaaaa agggatgacc aaagggatgc
    15601 atggtcgtgg tgctgaggac cttagagttc gagtaccaca aggtacgact gttcgtgatg
    15661 cggagactgg caaggtttta acagatttga ttgaacatgg gcaagaattt atcgttgccc
    15721 acggtggtcg tggtggacgt ggaaatattc gtttcgcgac accaaaaaat cctgcaccgg
    15781 aaatctctga aaatggagaa ccaggtcagg aacgtgagtt acaattggaa ctaaaaatct
    15841 tggcagatgt cggtttagta ggattcccat ctgtagggaa gtcaacactt ttaagtgtta
    15901 ttacctcagc taagcctaaa attggtgcct accactttac cactattgta ccaaatttag
    15961 gtatggttcg cacccaatca ggtgaatcct ttgcagtagc cgacttgcca ggtttgattg
    16021 aaggggctag tcaaggtgtt ggtttgggaa ctcagttcct ccgtcacatc gagcgtacac
    16081 gtgttatcct tcacatcatt gatatgtcag ctagcgaagg ccgtgatcca tatgaggatt
    16141 acctagctat caataaagag ctggagtctt acaatcttcg cctcatggag cgtccacaga
    16201 ttattgtagc taataagatg gacatgcctg agagtcagga aaatcttgaa gaatttaaga
    16261 aaaaattgtc tgaaaattat gatgaatttg aagagttacc agctatcttc ccaatttctg
    16321 gattgaccaa gcaaggtctg gcaacacttt tagatgctac agctgaattg ttagacaaga
    16381 caccagaatt tttgctctac gacgagtccg atatggaaga agaagcttac tatggatttg
    16441 acgaagaaga aaaagccttt gaaattagtc gtgatgacga tgcgacatgg gtactttctg
    16501 gtgaaaaact catgaaactc tttaatatga ccaactttga tcgtgatgaa tctgtcatga
    16561 aatttgcccg tcagcttcgt ggtatggggg ttgatgaagc ccttcgtgcg cgtggagcta
    16621 aagatgggga tttggtccgc attggtaaat ttgagtttga atttgtagac taggagactg
    16681 gtatgggaga taaaccgata tctttccgag atgcggatgg taattttgtt tccgccgcag
    16741 acgtttggaa tgaaaagaaa ttggaagaac tatttaatcg tctcaatcca aatcgtgcct
    16801 tgagattggc acgaactaaa aaggaaaatc catctcagta aagaagctaa aaaatcccgt
    16861 gcctcatcag acacgggatt ttgtggtacg acaggcatgt atagcaaact gaatctggaa
    16921 tagcacagca tatcttctaa aatattgcta taaattgatt tgaatttcaa aattaaattg
    16981 ttttattctt atttcaattt gttatagtat atctgatgtc aaagttctcg gcgagtcaaa
    17041 tagtgattcc caagcctgac tatcgtgagg tagcggatta aaatggtctg gggatagacc
    17101 gttttaagtc tgatgctgga aataagaatt gtcagaagaa gggatagcga aatcgtggct
    17161 ctacgaacag gaacgtgata ataaggcgta tatagcgaat aagagggcct caaactctaa
    17221 agtccaaaaa ggtagtcgta acctatatgc gtaaatcatg agagtaattg aattcgtact
    17281 aagattttct atttttactg taacctttta acgtcctcat atcttgtata cacgaggaaa
    17341 gatgtacgac ttatcccgtg aggtctatca ctataaagag aaaacgacag atagaagtga
    17401 tcctgagtca cggttatctt taaaatagat tgtctgatag gatgctagcc tgaaaacgtc
    17461 ttgttcatga aatataggac ggtatgtata aaacgcttct gtgaactgag agaaggggga
    17521 gaagttcttg ctaaaattta gttgaacagc cgtattccga tacttagata agagatctag
    17581 tcttagctcc tactcagttt caggggataa aaaaggggca atagcgattc gagaaagatt
    17641 atactcttcg aaaatctctt caaatcacgt caatatcgcc ttgccgtatg tgtaggatac
    17701 taactacgtc agttccatct acaatctcaa aacagtgttt tgagcaacct gcggctagtt
    17761 tcctagtttg atctttgatt tttattgagt attagtaatt cagttactaa ctcgtcaact
    17821 ctgatttatc caataaaatt gaaaaggatg gaaaaaagga taaatttatg atatacttta
    17881 ttttgaagac cttattagaa atcttgaaag agtattgaaa acttagaatg agaaaaattg
    17941 ttatcaatgg tggattacca ctgcaaggtg aaattactat tagtggtgct aaaaatagtg
    18001 ttgtggcctt aattccagct attatattgg ctgatgatgt ggtgactttg gattgtgttc
    18061 cagatatttc ggatgtagcc agtcttgtcg aaatcatgga attaatggga gctactgtta
    18121 agcgttatga cgatgtcttg gagattgatc caagaggtgt tcaaaatatt ccaatgcctt
    18181 atggtaaaat taacagtctt cgtgcatctt actattttta tgggagcctc ttaggccgtt
    18241 ttggtgaagc gacagttggt ctaccgggag gatgtgatct tggtcctcgt ccgattgact
    18301 tacaccttaa ggcgtttgaa gctatgggtg ccactgctag ctacgaggga gataacatga
    18361 agttatctgc taaagataca ggacttcatg gtgcaagtat ttacatggat acggttagtg
    18421 tgggagcaac gattaatacg atgattgctg cggttaaagc aaatggtcgt actattattg
    18481 aaaatgcagc ccgtgaacct gagattattg atgtagctac tctcttgaat aatatgggtg
    18541 cccatatccg tggggcagga actaatatca tcattattga tggtgttgaa agattacatg
    18601 ggacacgtca tcaggtgatt ccagaccgca ttgaagctgg aacatatata tctttagctg
    18661 ctgcagttgg taaaggaatt cgtataaata atgttcttta cgaacacctg gaagggttta
    18721 ttgctaagtt ggaagaaatg ggagtgagaa tgactgtatc tgaagacagc atttttgtcg
    18781 aggaacagtc taatttgaaa gcaatcaata ttaagacagc tccttaccca ggctttgcaa
    18841 ctgatttgca acaaccgctt acccctcttt tactaagagc gaatggtcgt ggtacaattg
    18901 tcgatacgat ttacgaaaaa cgtgtaaatc atgtttttga actagcaaag atggatgcgg
    18961 atatttcgac aacaaatggt catattttgt acacgggtgg acgtgattta cgtggggcca
    19021 gtgttaaagc gaccgactta agagctgggg ctgcactagt cattgctggg cttatggctg
    19081 aaggtaaaac tgaaattacc aatatcgagt ttatcttacg tggttattct gatattatcg
    19141 aaaaattacg taatttagga gcggatatta gacttgttga ggattaaacc gtagaggtgt
    19201 ttatgaatat ttggaccaaa ttagcaatgt tttctttttt tgaaacggat cgcttgtatt
    19261 tgcgtccttt cttttttagt gatagtcagg acttccgcga gatagcttca aatccagaaa
    19321 atcttcaatt tattttccca acgcaggcaa gtctggaaga aagtcaatat gcactggcca
    19381 attactttat gaagtcccct ttgggagtgt gggcaatttg tgaccagaaa aatcaacaaa
    19441 tgattggttc tattaaattt gagaagttag atgaaatcaa aaaagaagct gagcttggct
    19501 attttttgag aaaagatgct tggtcgcaag gatttatgac agaggttgtt agaaaaattt
    19561 gtcagctttc ttttgaggaa tttggcttaa aacaattatt tatcattacc caccttgaaa
    19621 atgaagctag ccaaagagtt gctcttaagt ctggatttag tttgttccgt cagtttaagg
    19681 gaagtgatcg ttacacaaga aaaatgcggg attatcttga atttcggtat gtaaaaggag
    19741 agttcaatga gtaagcatca ggaaattcta agctatttgg aggaattacc agtaggtaaa
    19801 agggtcagtg ttcgtagcat ttcgaatcat ctaggagtta gtgatggaac agcctatcga
    19861 gctattaaag aagctgaaaa ccgtggaatt gtggagaccc gtcctagaag tggaacaatt
    19921 cgtgttaaat cccagaaagt tgctatagag agattaacgt ttgctgaaat tgcagaagtg
    19981 acttcttctg aggttctggc tgggcaagaa ggtttagaga gagagtttag taagttttca
    20041 attggtgcca tgactgaaca aaatatcttg tcttaccttc atgatggggg gctcttgatt
    20101 gtcggagacc gaacccgtat tcagttgcta gccttggaaa atgaaaatgc agttctggtt
    20161 acagggggat ttcaggttca tgatgatgtg cttaaactgg ccaatcaaaa agggattcct
    20221 gttctaagaa gtaagcatga tacctttacc gtcgcgacca tgatcaataa agccttgtca
    20281 aatgtccaaa tcaagactga tattctgaca gttgagaaac tttatcgccc tagtcatgag
    20341 tatggttttc tgagagagac agatacagtt aaagattatt tggacttggt tcgtaagaat
    20401 cgtagcagcc gtttccctgt tatcaatcaa catcaggtcg ttgttggtgt tgtaaccatg
    20461 agagacgctg gtgataaatc accaagcacg acaattgata aggttatgtc tcgtagtcta
    20521 ttttgggttg gattatcgac aaatattgcc aatgtgagtc aacggatgat cgcagaagac
    20581 tttgaaatgg taccagttgt tcgaagcaat caaactttgc ttggcgttgt gacgcgacga
    20641 gatgtcatgg agaagatgag ccgttcccaa gtttcggctc taccaacttt ttctgagcag
    20701 attggacaaa agctctctta tcaccatgat gaagtagtca ttacagtgga accctttatg
    20761 ctagaaaaaa atggagtttt ggctaatggt gtattggcag aaattctgac ccacatgacc
    20821 caagatttag ttgttaatag tggtcgcaat ctcattatcg agcagatgct gatctacttt
    20881 ttgcaggctg ttcagataga tgatatattg cgcattcagg cacggattat tcatcatacg
    20941 agacggtcag ctataattga ttacgatatt tatcatggtc accagattgt ttcaaaagca
    21001 aatgtgactg ttaaaattaa ttagaaacta ggagaaaaga tgataacatt aaaatcagct
    21061 cgtgaaatcg aagctatgga caaggctggt gattttctag caagtattca tataggctta
    21121 cgtgatttga ttaagccagg cgtagatatg tgggaagttg aagaatatgt ccgccgtcgt
    21181 tgtaaagaag aaaatttcct tccacttcag attggggttg acggtgccat gatggactat
    21241 ccttatgcta cctgttgctc tcttaacgat gaagtggctc acgctttccc tcgtcattat
    21301 atcttgaaag atggtgattt gctcaaagtt gatatggttt tgggaggtcc cattgctaaa
    21361 tccgacctga atgtctcaaa attaaacttc aacaatgttg aacaaatgaa aaaatacact
    21421 cagagctatt ctggtggttt agcagactca tgttgggctt atgctgttgg tacaccgtcc
    21481 gaagaagtca aaaacttgat ggatgtaacc aaagaagcta tgtacaaggg tattgagcaa
    21541 gctgttgttg gaaatcgtat cggtgatatc ggtgcggcta ttcaagaata cgctgaaagt
    21601 cgtggttacg gtgtagtgcg tgatttggtt ggtcatggtg ttggcccaac tatgcacgaa
    21661 gaaccaatgg ttcctaacta tggtattgca ggtcgtggac tccgtcttcg tgaaggaatg
    21721 gtcttaacca ttgaaccaat gatcaataca ggcgattggg aaattgatac agatatgaaa
    21781 actggttggg cgcataagac cattgacggt ggattgtcat gtcagtatga acaccaattt
    21841 gtcattacga aagatggacc tgttatcttg actagccaag gtgaagaagg aacttattaa
    21901 taaaaagtga aaagactact ggaagtttat tttgataaaa aatccagtag atcttttcat
    21961 aataaaacgc attgtatcaa gtgttaggag ctgatatcat gcgtttttct gcttttaaga
    22021 ttttttccaa ctctgtttgt aagcgcatca taacaaaggg tctaggattc agggctctcc
    22081 tcctatatac tattagtaaa gtaaaactaa gagaggatat tttagtgtcg cagtctattg
    22141 ttcctgtaga gattccacaa tattgtcgtt ttgattctaa aaagagaaat ggaattctgt
    22201 ttaacgttcg tattgccaat cttaaattta ccttttttag attagacttc ctgcgaaata
    22261 aaatatggta tagtagttct atgaatgatg aagcaagtaa acaactaact gatgcacgat
    22321 ttaagcgtct tgttggtgtt cagcgcacga cttttgaaga gatgttagct gtattaaaaa
    22381 cagcttatca acttaaacac gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc
    22441 ttcttatggc cactcttcaa tatgtgcgag aatatcgaac ttatgaagaa attgcggctg
    22501 tttttggtat ccacgaaagc aacttaatcc gtcggagcta atgggttgaa gtaactcttg
    22561 ttcaaagtgg tgttacgatt tcaagaactc ctctcagctc tgaggccacg gtaatgattg
    22621 atgcgacgga agtaaaaatc aatcgcccta aaaaaacaat tagcgaatta ttcttgtaaa
    22681 aaaatgccac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttcttt
    22741 ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtt gcagaaatat
    22801 cggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc
    22861 tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagttg aagataaagc
    22921 ctgtaaccat gcgctatcca aggagagaag caaggttgag aacatctttg ccaaagtaaa
    22981 aacgtttaaa ataatttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat
    23041 gaatttgagt gctggtatta tcaatcatga actaggattc tagttttgca ggaagtctat
    23101 tgaggtattg agctagttta tgaaaaaatt gggtgaaaag tcgagtgttt tagaaaccca
    23161 cagtgtagta ttctagtttc aatccactat attttgctac tccccgtaaa gtttctattt
    23221 tccctgattt ctgatataat agaaatattg acttcaagag taaggaagag aagatgaacg
    23281 cattattaaa tggaatgaat gaccgtcagg ctgaggcggt gcaaacgaca gaaggtccct
    23341 tgctaatcat ggcaggggct ggttctggaa agactcgtgt tttaacccac cgtatcgctt
    23401 atttgattga tgaaaagctg gtcaatcctt ggaatatctt ggccattacc tttaccaaca
    23461 aggctgcgcg tgagatgaaa gagcgtgctt atagcctcaa tccagctact caggactgtc
    23521 tgattgcgac cttccactcc atgtgtgtgc gtattttgcg tcgcgatgcg gaccatattg
    23581 gctacaatcg taattttaca attgtggatc ctggtgaaca gcgaacgctc atgaaacgta
    23641 ttctcaaaca gttgaacttg gatcctaaaa aatggaatga acgaactatt ttggggacca
    23701 tttccaatgc taagaataat ttgattgatg atgttgctta tgctgcccaa gctggcgata
    23761 tgtatacgca aattgtggcc cagtgttata cagcctatca aaaagaactt cgtcagtctg
    23821 aatccgttga ctttgatgat ttgattatgc tgaccttgcg tctctttgat caaaatcctg
    23881 atgttttgac ctactaccag caaaaattcc aatacatcca cgttgatgag taccaagata
    23941 ccaaccacgc tcagtaccaa ttggtcaaac tcttggcttc ccgttttaaa aatatctgtg
    24001 tggttgggga tgcggaccag tctatctacg gttggcgtgg tgctgatatg cagaatatct
    24061 tggactttga aaaggattac cccaaagcca aggttgtctt gctggaggaa aattaccgct
    24121 caaccaaaac cattctccaa gcggccaacg aggttattaa aaataataaa aatcgccgtc
    24181 ctaaaaatct ctggactcaa aacgctgatg gggagcaaat cgtttactat cgtgccgatg
    24241 atgagctgga tgaggctgta tttgtagcca gaaccatcga tgaacttagt cgcagtcaaa
    24301 acttccttca taaggatttt gcagttctct atcggactaa tgcccagtcc cgtacaattg
    24361 aggaagccct gctcaagtct aacattcctt ataccatggt tggcggaacc aaattctaca
    24421 gccgtaagga aattcgcgat attattgctt atctcaacct tattgctaat ttgagtgaca
    24481 atattagttt tgagcgtatt atcaacgagc ctaaacgtgg aattggtcta ggtacagttg
    24541 agaaaatccg tgattttgca aatttgcaaa atatgtctat gctggatgct tctgctaata
    24601 ttatgttgtc tggtatcaag ggtaaggcag cccaatctat ctgggatttt gccaatatga
    24661 tgcttgattt gcgggagcag ctagaccact taagcattac agagttggtt gagtccgtcc
    24721 tagaaaaaac aggttatgtc gatattctta acgcccaagc gactctagaa agcaaggcac
    24781 gggttgaaaa tatcgaagag tttctttctg ttacgaagaa ctttgatgac accacggatg
    24841 tgacagaaga ggaaactggt ctggacaaac tgagtcgttt cttaaatgac ttggctttga
    24901 ttgccgacac agattcaggt agtcaggaga catcagaagt gaccttgatg accctgcatg
    24961 ctgccaaagg tctcgaattt ccagttgtct ttttgattgg gatggaagaa aatgtctttc
    25021 cacttagtcg tgcgactgaa gatccagatg aattagaaga agagcgccgt ctagcctatg
    25081 taggtatcac gcgtgcagag aaaattctct atctgaccaa tgccaactca cgcttgcttt
    25141 ttggtcgtac caattataac cgtccgactc gttttattaa cgaaatcagt tcagacttgc
    25201 ttgagtatca aggtctggct cgtcctgcaa atacaagctt taaggcatca tatagcagtg
    25261 gtagtatttc ctttggtcaa ggtatgagtt tggctcaggc tcttcaagac cgtaaacgcg
    25321 gtgctgcccc aaaatcaatc cagtcaagcg gtcttccatt tggtcaattt acagctggcg
    25381 caaaaccagc atctagcgag gcaaattggt ccattggtga tattgctctc cacaagaaat
    25441 ggggagaggg aaccgttctg gaagtttcag gtagcggtgc taggcaggaa ttgaaaatca
    25501 atttcccaga agtaggtttg aaaaaacttt tagccagtgt ggctccaatt gagaaaaaaa
    25561 tctaattttc catccttctc acgaataata aagtgaggag gatttttatg tacagtattt
    25621 cattccaaga agattcacta ttaccaagag aaaggctggc caaggaagga gttgaagcgc
    25681 ttagtaacca agagttgcta gctattttac tcaggacagg aacacgtcaa gttagcgttt
    25741 ttgaaattgc ccaaaaagtc ttgaacaatc tttcaagcct aacggatttg aaaaaaatga
    25801 ccctgcagga attgcagagt ttgtctggta ttgggcgtgt taaggccata gaattacaag
    25861 ctatgattga actggggcat cgtattcaca aacacgagac tcttgaaatg gaaagtattc
    25921 tcagcagtca aaagttggcc aagaagatgc agcaggaatt aggggataaa aaacaagagc
    25981 acctggtggc actctatctc aatactcaaa atcaaatcat ccatcagcag accattttta
    26041 tcgggtctgt aactcgtagt atcgctgaac cgcgagagat tcttcactat gcaatcaagc
    26101 atatggcgac ttctcttatc ttggtccaca atcatccttc aggagcggta gcgcctagcc
    26161 aaaatgatga tcatgtcact aaacttgtta aagaagcctg cgaattgatg gggattgttc
    26221 tcttggacca tttgattgtc tctcattcta attactttag ttatcgtgaa aagacagatt
    26281 taatctaaag ttcattaacg acatagtcaa agagtttttt atctttggga cgattttcaa
    26341 aaagaagttc tggatgccat tggacaccga gaaaggcgac atcatccgta ctcatgacag
    26401 cctcaatgat accatcttta ggatcatgag ccacaacttt taaatttggt gctaagtcct
    26461 tgatgctctg gtggtggaag gagttgatat gagagatttc tccatagatt tcttggagaa
    26521 cggtatctgg ttctgttacc aagcgttgag ttgtgtactc aacagaagaa tcctgccaat
    26581 ggtcttcgat atcttggtac aaagttccac ccatggcaac gttaaagagt tgggtaccac
    26641 ggcagacaga gaaaatgggc tttttctgtt taatagcttc cttgatgagg gtcagttcga
    26701 agatatctct ttgaaggtga tagtcatcgc tatcaatggt tttgggttcg ccataaaatt
    26761 ttggatcgac attttgccca cctgtcaaga tgagcttgtc aatcaaactg atatagtggc
    26821 aggccatttc ttgatcacca atcggtagga tgatgggaat ccctccagca tctttaacgc
    26881 cttcaacaaa gccttttgct gcgtagctca tcatgatgtc atcatctgga tgagcttttt
    26941 cgtttcctgt aatcccaata actggttttt tcataaaatg attttcgctt tctaatcctc
    27001 ttttcgcatg aagtagagga gggtttggag ttcacttgtc aaatcgacat actgaacgac
    27061 cacgtctttt ggtaaatgca gatggactgg tgaaaaactg agaattcctt tcacaccagc
    27121 atcaaccaag agattagcaa cctcttgtga cttgacgctg ggaacagtta ggatagcagt
    27181 cttcacatca gcatccttga ttttatcctt gatctgagaa atcccgtaaa tgggaatccc
    27241 gtcaggagtt tgggtaccga cttcaggatg gtcgtctagg tcaaaggcca tgataatctt
    27301 catcttgtta cgttcgtgga agcggtagtg gagaagggca tggcccatat ttccaatacc
    27361 aaccagcatg acattggtaa tagagttgtc attgagcaaa tcggcaaaaa atgtcattag
    27421 ttttttgaca tcatagccaa aaccacgacg accaagttca ccaaaatagg aaaaatcacg
    27481 acgtacggtc gctgaatcaa taccgatagc ctctgcaatt tgcttagagt tggcacgttc
    27541 aatcttttct gcatgaaatc tcttaaaaat tcgatagtag agagagagtc tttttgctgt
    27601 agcttttgga atagcaaact gtttatcttt cacaaaatca ca
//
LOCUS       DAVLFB010000017        27540 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLFB010000017 DAVLFB010000000
VERSION     DAVLFB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.017"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(576..1802)
                     /locus_tag="VXD58_000655"
     CDS             complement(576..1802)
                     /locus_tag="VXD58_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV0675398.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1845..2537)
                     /locus_tag="VXD58_000656"
     CDS             complement(1845..2537)
                     /locus_tag="VXD58_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0675399.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2710..4461)
                     /locus_tag="VXD58_000657"
     CDS             complement(2710..4461)
                     /locus_tag="VXD58_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675400.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4451..6196)
                     /locus_tag="VXD58_000658"
     CDS             complement(4451..6196)
                     /locus_tag="VXD58_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675401.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6324..6854)
                     /locus_tag="VXD58_000659"
     CDS             complement(6324..6854)
                     /locus_tag="VXD58_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV0675402.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            complement(6851..7307)
                     /locus_tag="VXD58_000660"
                     /pseudo
     CDS             complement(6851..7307)
                     /locus_tag="VXD58_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7441..8268
                     /gene="xth"
                     /locus_tag="VXD58_000661"
     CDS             7441..8268
                     /gene="xth"
                     /locus_tag="VXD58_000661"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV0675403.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8280..8378
                     /locus_tag="VXD58_000662"
     CDS             8280..8378
                     /locus_tag="VXD58_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0675404.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            8423..8662
                     /locus_tag="VXD58_000663"
     CDS             8423..8662
                     /locus_tag="VXD58_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV0675405.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9045..9140
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9540..10121
                     /locus_tag="VXD58_000664"
     CDS             9540..10121
                     /locus_tag="VXD58_000664"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV0675406.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10121..11383
                     /locus_tag="VXD58_000665"
     CDS             10121..11383
                     /locus_tag="VXD58_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV0675407.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGNGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11434..11895)
                     /locus_tag="VXD58_000666"
     CDS             complement(11434..11895)
                     /locus_tag="VXD58_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV0675408.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11892..12656)
                     /locus_tag="VXD58_000667"
     CDS             complement(11892..12656)
                     /locus_tag="VXD58_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV0675409.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12837..13241)
                     /locus_tag="VXD58_000668"
     CDS             complement(12837..13241)
                     /locus_tag="VXD58_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV0675410.1"
                     /translation="MKDFHFDAISAFESYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(13311..14792)
                     /locus_tag="VXD58_000669"
     CDS             complement(13311..14792)
                     /locus_tag="VXD58_000669"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0675411.1"
                     /translation="MILVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(14811..15989)
                     /locus_tag="VXD58_000670"
     CDS             complement(14811..15989)
                     /locus_tag="VXD58_000670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV0675412.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            16154..17161
                     /gene="galR"
                     /locus_tag="VXD58_000671"
     CDS             16154..17161
                     /gene="galR"
                     /locus_tag="VXD58_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV0675413.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQGLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(17208..18245)
                     /locus_tag="VXD58_000672"
     CDS             complement(17208..18245)
                     /locus_tag="VXD58_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0675414.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(18246..18599)
                     /locus_tag="VXD58_000673"
     CDS             complement(18246..18599)
                     /locus_tag="VXD58_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV0675415.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(18843..19733)
                     /locus_tag="VXD58_000674"
     CDS             complement(18843..19733)
                     /locus_tag="VXD58_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV0675416.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            19866..20402
                     /locus_tag="VXD58_000675"
     CDS             19866..20402
                     /locus_tag="VXD58_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0675417.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(20569..21375)
                     /locus_tag="VXD58_000676"
     CDS             complement(20569..21375)
                     /locus_tag="VXD58_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV0675418.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(21720..23240)
                     /locus_tag="VXD58_000677"
     CDS             complement(21720..23240)
                     /locus_tag="VXD58_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV0675419.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(23233..23961)
                     /locus_tag="VXD58_000678"
     CDS             complement(23233..23961)
                     /locus_tag="VXD58_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675420.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(23976..24740)
                     /locus_tag="VXD58_000679"
     CDS             complement(23976..24740)
                     /locus_tag="VXD58_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV0675421.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            complement(24744..25187)
                     /locus_tag="VXD58_000680"
     CDS             complement(24744..25187)
                     /locus_tag="VXD58_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0675422.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(25869..26165)
                     /locus_tag="VXD58_000681"
     CDS             complement(25869..26165)
                     /locus_tag="VXD58_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0675423.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(26187..27251)
                     /gene="pepA"
                     /locus_tag="VXD58_000682"
     CDS             complement(26187..27251)
                     /gene="pepA"
                     /locus_tag="VXD58_000682"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV0675424.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         8103 a         6328 c         5138 g         7971 t
ORIGIN      
        1 ccttctataa aagctagatt taacctcgat aaaaatagat gcagtgcttc taaaatactg
       61 gaatagggca tataggtaat cgtagacaag tgttttatag attgtctcgc aggagatcta
      121 atgatgtata gcaagcatca ctaaacatat gggatatttg cgttattata atatcaacat
      181 gctataaatt ctatcatgca aaagaatgac tagaacatga aagacaggca caaaactttg
      241 aacaatattc tgaaaacaaa aaaaagacgc tagtttccac aatctcactc atggaaactc
      301 acgtctttca aaaaataggg tgaattgaaa actagaacaa gacactagat tttctaaatg
      361 atgtctagta aggattctaa atgctatacc aaattgctca catcttattt caaaccacgg
      421 gactcccaca agaaaggaaa atcctataaa aattctctct ctgaggagaa agaggcctca
      481 actacaggtg taaactatta tcaattgaaa acaacagttt ttacactcaa ttagactttt
      541 tagggacagg atcactttcg cttgactgta gtttgtcatt tttttgataa agttagcact
      601 ttttcagaaa ctgttttgaa agtctcaatg atatagtcta cttcttcatc gcttaattta
      661 gtatgaagag ggagggtaat ttcattctca aagaaggcat aggccttagg atagttcgtc
      721 atatcaaatc caagattctt ataggctgtc aagagaggaa gcggtttgta gtgaacatta
      781 cttgcaattc ctgctttagc caattcttgg atgatgaggt tgcgttcttc taggcttgct
      841 ccttctacac gggtgatgta gaggtggcgt gaagattcga cagtttcagt cttgtgtgcc
      901 aaaggatgga tgcgagaacc tgcaaaacca ctatcatagc ggtccacaat gtccttacgg
      961 cgttgcaaca aacttggata gcggtccaat tgtaccaaac caagtgaagc catgatatcg
     1021 gtcatgttgc acttataggc tggtgtaacg atatcgtatt cccatgaccc cagttgcatc
     1081 ttggcaagag catccttagt ttgcccgtga agggaaagga tttggaattc cttgtacatc
     1141 tcttcgtcat caatcactgg attggctttc caagtcgcac ttccaccttc tgccgttgta
     1201 aagttcttaa cagcatggaa tgagaaggaa gtaaaatcag cgatagaacc agaaggttgt
     1261 cctttataag tagatcccaa agcgtgggca ctatcagaga caatgacaat acggttaaag
     1321 gccttttgcc acttgcttga agcggtaaag aagtcacgtt ttttctccac gacttggaac
     1381 aaacggtcat aatcgcaaac aatccctgcg agctctactg ggataatcac cttagttttc
     1441 tcagtgatag cttgctcaag caggtcatag tccatctcaa acgtatctgc ttggatatcc
     1501 accatgacag gggttgctcc cacgtgcgta atgacactac atgaagccgt ataggtcatg
     1561 gctggaacga tgacttcatc accaggtccc acttccaaaa cgcgtaaaat caactccaga
     1621 gcggctgtcg cagagttgag acaaacagtc ttaggtgtct gtgtgtaaag agacaagcgg
     1681 cgctccagtt cttttgtttt aggacctgtt gtgatccaac cagaacgcag ggtatccgct
     1741 acttcagcaa tttctgcttc tgtgatatca ggcggtgaaa atggaatatt gtaatttggc
     1801 attgatttgc tccttttatc tgtactcatt ttcttatgac tactttattt tagtacctca
     1861 aacacggttt gaaacatgat tttgatgtcc ccaaagaaac taaactctcg gagataggcg
     1921 aggttatagc gcatcttttc aggaagaaca tgctccacat aggcctgatc aactgacaga
     1981 cctttctccg tcatttgact gatgatggtg tcctcatcct tgtagttgat gctggctgga
     2041 gaggtaattc ctgcttgcaa gagcaaggtt gccatcattt cagggctata ctgctctgta
     2101 taacgtggca cttcaggtcg tgtaccgaca aaggacatct cacctttaag gacattgacc
     2161 aactgaggca gttcgtccaa acggacacgt cggatgaaat ttccaacctt ggtaatacgg
     2221 ctatcgttag cagaagtcac cagacttcct tttttatccg catccgtcac catggtacgg
     2281 aacttccaaa tcttgaaccg acggttgtac tgggtcacac gctcttgctt gtaaatcact
     2341 ggccccttgc tatccaactt gatccaaatg ctcaagatga gaaagatggg agaagttaga
     2401 accagtaaga ccaaggccaa aacccagtcc aagcaacgct tgaaaatcag cgaacccttc
     2461 cttttagaga caagctggta gtaagactca acctcgcttg atttcatttc cacaggcaag
     2521 tcttcccatt tcagcatgct gttctccttt gtttttatta tactatttgt caacattttt
     2581 cattatacca caaaatggaa aaggcggtga aagaaatctg taatttgaag gatttccttc
     2641 tagtactcaa tgaaaatcaa agtacagctt tgagattgca gataaagctg acatggttta
     2701 aagagatttt caaagagtat aggccatggc ccctgcctgc aaactataca tcttgtgata
     2761 ggttcctccc agagtcaaga gttcctcatg ggttccactc tcgataatgc gtcccttatc
     2821 caagacatag atgcagttgg cgtcttgaat agtagaaagg cggtgagcga tagcaatagt
     2881 tgttcggccc tgtctcatct tcgccagaga agcttgaacc aaactttctg tttcagagtc
     2941 aatattggct gtcgcttcat ccaaaatcag gattttaggc tggctggcga ctgttctagc
     3001 aaaggcaaga agctggcgct gcccagtaga gaagctcgaa ccacgctcgg aaacagggga
     3061 gtcgtacccc tgaggaagtt cttgaataaa ggaatctgca tccacaaagg ctgccgcagc
     3121 ctgaacctgc tcatcactga tttcttggta catggcgata ttggacttaa tagttccatg
     3181 atagaggaag ggttcctgta aaaccaaacc gatgtttttt ctcagctctt cttgactgaa
     3241 atccctgata tccacatcat ccaagagaac tctccctgac tggaattcat aaaagcgcat
     3301 gaggacattg ataatagacg atttccctga acctgtatga cctacaaagg caatggtttc
     3361 acccttatta acagagaaag aaatgtcatc cagaatcgga tgtttaccgt catacgagaa
     3421 acacacatgt tcaaaacgga tattgccttc tttgactttg gcttgcccat tttcttgaag
     3481 gggctcatag gtcctctcat cgattaaggc aaagacacgg cctgcagaaa ccatagacgt
     3541 ttgcagtgtt gaaaagtttt gcgtcacctc aatcaaaggg tcaaagaggc ggttgatgta
     3601 ctggataaaa gcatacatgg tcccggccgt tatcccgata gaaagaccac gatagccaaa
     3661 ataggtcatc aagacagcat aacctagaag tttcaacaaa ctcatggcag gtctcaaaaa
     3721 gagggcatcc aaggctacag aacggttggc ataggccaag tgctcttggt tgatttcatc
     3781 aaattctgcc tgcaggcgct tctcttgatt aaaagcctga ataatcctga ttccctcgat
     3841 attctctgcc agcttactat tgatatctga caagagactt ctggttttct cgatgatttt
     3901 cactgacttt tttcgataga gattgaccaa aaggaaaatc aaggggagaa agagcaagac
     3961 taaagccgtc aaacgaaaat ccagcaccaa catggtataa agggttgtca gaaagataaa
     4021 aactgctgag ataaagctgg ataaaatccc agaaaacata tcactaatcg tctcggtatc
     4081 gttggtcaaa cgagaaacga tagaacctgc tggcgtcttg tcaaagtaag acatgcccag
     4141 tttctccata ttggcaaagg catcccgacg aatatcccta acaatactgt aagacacgcg
     4201 cgcaaagaga agattgccga catactgaac tacagtttgt aggatgtaga gaccatagta
     4261 gaccagcaaa acggtcacgg ctagttggtt aagattgctg agatactggt cgataaagtg
     4321 ggaagccaca aggggaatga cacttttaat gaccgtcgtc gctaggagaa aactgagtgc
     4381 caaaaaggta aggagtccat aaggcttgag ataggacatc aagcgcttca atacagtcca
     4441 ttgttcttgt ttattctgca tcttcttctc ccttcatttc cagctgctga gactggtagg
     4501 tttgggcata ccagccatcc aaagctagca agtcttcgtg cctgcctcgt tcgataattt
     4561 gaccattttg caggactaaa atcaaatctg catggacaac tgcactcagg cgatgggcag
     4621 tgataatggt tgtcttgtcc tttcgcgtct ccttgaggtt gtcgataatc gcatactctg
     4681 tcttggcatc tacggcggat aaggaatcat ccaaaatcaa gatatcaggg tctaaaatca
     4741 tagcccgact catagccaac cgttgctttt gaccaccaga aagactgact cctttttcac
     4801 caatcagcgt atcaaatcct tgaggcatgt ctacaatatc ttggtaaact cgggctagct
     4861 tagtagcttc ctcgaccgct gaaaggggca agttaggatt gccaaagcgg atattgtcta
     4921 ggattgaagt cgcaaaaaga aactggtcct gaggaacata gcccatgaga ctgcgaaggt
     4981 ctgtcagacg atagtcccga atatcgtgac cgtttagata aatggcgccc ttatccacat
     5041 cgtattcacg caagaggagc ttgattaagg acgttttccc agagcctgtc tgcccaacca
     5101 agcccagtgt ttgccctttt gccaaactaa agtgaatatc cgtcagtgtt tcctcatttt
     5161 caaaagcaaa gctgtcaatg gcatactcca aacgcccatt ttcaatacca tccagaggaa
     5221 actccgggtc ttgtacagga gattcctgag acaaaagatt ttcaatccgc tggtaagaaa
     5281 ccttccctcg ctgagtagta ttaaagagga agccgatggc cataagaggc cagaccagca
     5341 tatccaaata gctgataaag gtgactagat tcccaactgt aatctgccct tcctgaacca
     5401 ccaaggagcc aaccaaaagc gttaaaacat aggacgaacc aacaaacaag agaaccatag
     5461 ggtcaaagag actatcatat ttcatggttt gcaggttctt ttggaaggtt aattcattga
     5521 ctgcctgaaa agacttcaac tcgtctgcct gataaccgaa agacttggtc actttgatac
     5581 ctgatacgga ctcctgtacc ttgttattga gttcagaaaa agcagcttgg gattcgccaa
     5641 aggccttatg agtctttctc cctaggcgac tagtcgcata ggccatgaaa ggtaggggga
     5701 gaatggcaac aagagtcatc tgccatgaga tgctaaagag catggtcaac aaagtcacca
     5761 gagccgtgat agaggcatcc accgcagaca tgacaccgcc acctgctaaa cgagtcaagg
     5821 cattgatatc attggttgcg tgtgccatca gatcacccgt ccgataggtt tgataaaagg
     5881 ctgacgacat ttttgtgaaa tgcttaaaca agcgagaccg catgatctgt cccaagcaat
     5941 aagaggtccc aaggatatac atacgccaca catagcgcaa atagtacata ccaaaggctg
     6001 caagtagcaa gtaaaatagg ctaagaagga ggtcctgctg ggttaattgc cccgatgtga
     6061 tggcatcaat aacccgcccc ataaccatag gaggaatgag attgaggacg gaaaccaaga
     6121 ccagggccac aattccgact agataacggc gtttttctaa cttgaaaaac caccaaagtt
     6181 tttgaataat ggacataaaa tccctttctg attgcaaatg gaaacctgag gccaatactc
     6241 aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaagc actgctttga
     6301 ggttgtagat agaactgacg aagtcagtaa cctacatacg gcaaggcgac gttgacgcgg
     6361 tttgaagaga ttttcgaaga gtacaagacc ccaggttttt ctttttcata agttacgact
     6421 gtaacagcac ccttgtcata ttcagcaata aagatattgg ctacattgtc atgcccttgt
     6481 ttactgaggt tatcaagcaa ccactcttcg ctacgaccaa tcgattccaa gacatctact
     6541 tgaatcacac cgtcagtcac aactggatac ttaggatttt catctcccat ttgcacaacg
     6601 ataagttgcc cattttgctc ttgcacagct cgtttgactt gcttcatctg gaaaatccct
     6661 tggctacgaa gtttgagggc tacatccgat gcagacaaac caactgaacg acaggcttct
     6721 gggtcaatat tcccattttt gataaggaga gttggcttac catcaatcaa acgtttcaca
     6781 aagtgaacat tgttgttgag ccatttgaga gtcaagacca gtttgattaa aatttcgata
     6841 taattaagtg tcatttgttc acatccacca gttcaattgc gtctgtctta tacaattcga
     6901 ctttctctaa caggtacttg tctggctcac ttccgtttag ggcacgataa tagcgatctc
     6961 ccaccttgat aagtgcgcca tctgtgtttg tggaagtatt aacatagact tctgacttgt
     7021 ctactcccaa atctttggaa acaacctcga tgaaatgaag tgaagtttga aatttattgt
     7081 tagaaacttg attgttttga taagtcgtga tgctgaccaa aagcagagct aaataaggcc
     7141 agagctgaaa tcatgaccaa ctcacgaaat tcggttccct ttttatcatg gtatgccttg
     7201 aaagcaaaaa aaactgtcac agctaataga acaatcccaa agccaaccat gataccattt
     7261 tgctgaccga tttggctgag tacatagtca taagagtaga atttcatata ttttcactcc
     7321 ctttcataaa atcattctta attataaacg aaagagagta atttttcaaa ccatacgttg
     7381 gggaaataga tctttttttg gtataataaa atctataatc tgaatgaaaa aggtaacttt
     7441 atgaaactta tctcatggaa tattgattcc ctaaacgctg ccctaactag tgactcagct
     7501 cgtgccaaat tgtcccaaga agtcctacaa accttggtcg ctgaaaatgc tgatatcatt
     7561 gctatccaag aaaccaagct ttctgccaaa ggacctacaa agaaacacgt ggaaatttta
     7621 gaagaactct tcccaggcta cgaaaacaca tggcgctctt cccaagagcc tgcccgtaaa
     7681 ggctatgctg gaaccatgtt cctttataag aaagaactta cgcctacgat cagttttcca
     7741 gaaatcggtg ccccttctac catggacttg gaaggccgta tcatcactct agaatttgat
     7801 gcatttttcg taacccaagt ttacactcca aacgctggtg acggtctcaa acgcttggaa
     7861 gaacgccaag tctgggatgt taaatatgct gagtatttgg ctgaactaga caaagaaaaa
     7921 ccagtccttg cgaccggtga ctacaacgta gcccacaatg aaatcgacct tgcaaatcct
     7981 gctagcaacc gccgttcacc tggatttact gacgaggaac gtgctggatt taccaacctt
     8041 ttggcaactg gatttacaga caccttccgc catgttcatg gcgatgttcc tgaacgctac
     8101 acttggtggg cacaacgcag caaaacttct aaaatcaaca atacaggctg gagaatcgac
     8161 tactggctca caagtaaccg tatcgctgac aaggtcacta aatctgatat gattgactca
     8221 ggtgctcgcc aagaccatac accgattgtc ttggaaattg acttgtaagg attttctata
     8281 tggactacaa tgcggtcatt cccgagtttc ttgtatcgaa tatcgaacag tcccggtcct
     8341 tctactgtgg tttactgggt tttaggattg aatactaacg tccagaagaa aactttctct
     8401 ttctcttgaa gagtgtcaac taatgttaga ggagggaaca aaggatcaac tagctgaact
     8461 gacctacccc tttggtcgtg gagtcaatct ctcctttggc ataaaggatg ttccaaaact
     8521 ctatcaaaaa gtgatggagg caaactatcc tatttatcgt ctcttgacca aaagaaagtt
     8581 tcgtgtcagt gatccctata tctatcctca taaatttgca gttttggatc cagatggcta
     8641 ttttttaaga tttagcgagt agaataataa aaattgggaa aagtctttaa agttagaaaa
     8701 aggcatattg tcaggtgtct aaaaacttga tagtatgcct tttatgatgg aaaaatcttc
     8761 taatactctt cgaaaatctc ttcaagccac gtcaacgtcg ccttaccgta ggtatatgat
     8821 actgactctg tcaattctat ccacaacctc aaaacagtgt tttgaggttg tggatagctt
     8881 cctagtttgc tctttgattt tcatttagta taaatgttag cttacaatgg agtttttgtt
     8941 caccctctct tatttttgtt cgctttttaa accaaaacac taacaaaata gttgatttct
     9001 aaagttattt agtgtaaaat aaaaacattg gctcaagcct atttaaaatt gaatatcgtt
     9061 ttacttgttt atgtcgtgaa ttggcacgac gtttctacaa ggtgccggaa cacctaacaa
     9121 taagtaagtc agcagtgaga tatggccgtt tttgccatgt ctatttaggg gacttactta
     9181 gagattaagt aggtcttttt tgaatatttt taatatttcc cttaattagt gcggacggga
     9241 ggtgaaatta tcctgtgaat aatttcagga atccagggaa tttcatccct aatttttgag
     9301 cccctagctc tttcatttct aggcttaggc taaaatagtt ccccgaacta ttttactctc
     9361 aaaaatcccg tcatagacaa taacaagatt attgtctatg ataccactac aacgggaaat
     9421 atttgataag actcgcttcg ctcgtaatag ttttatcaaa tatatagtat tagcaaaagg
     9481 gatcactata gaaatattta aacaacaagt aaattaactt ttagacttta ggaggtctta
     9541 tgaaattatt agaagagcgc atcctcaagg atgggcatat cttgggtgat aacatcctca
     9601 aggtagattc ctttttaacc caccaagttg actttagctt gatgcgagag attggtaagg
     9661 tttttgcgga aaaatttgct gctactggca ttaccaaggt cgtaaccatt gaagcgtcgg
     9721 gtattgcccc agccgttttt acagctgaag ccttaaacgt tcccatgatt ttcgccaaaa
     9781 aagctaagaa catcaccatg aacgaaggca tcttaactgc tcaagtctac tcctttacca
     9841 agcaggtgac cagcactgtt tctatcgctg gaaaattcct ctcaccagag gacaaggttt
     9901 tgattatcga cgatttcctt gctaatggcc aagctgctaa aggcttgatt caaatcatcg
     9961 aacaggccgg tgccacagtc caagctatcg gtatcgtgat tgagaaatcc ttccaagatg
    10021 gtcgtgattt gcttgaaaaa gcaggctacc ctgtcctatc acttgctcgc ttggatcgtt
    10081 ttgaaaatgg tcaggtcgta tttaaggagg cagatctcta atgcaaactc aagaaaaaca
    10141 ctcgcaagca gccgttctgg gcttgcagca cttactagcc atgtactcag gatctatcct
    10201 ggttcccatc atgattgcga cagcccttgg ctattcagct gagcagttga cctacctgat
    10261 ttctacagat atcttcatgt gtggggtggc aaccttcctc caactccaac tcaacaaata
    10321 ctttgggatt ggactcccag tcgttcttgg agttgcattc cagtcggtcg ctcccttgat
    10381 tatgattggg caaagccatg gtaatggcgc tatgtttggt gcccttatcg catctgggat
    10441 ttacgtggtt cttgtttcag gcatcttctc aaaagtagcc aatctcttcc catctatcgt
    10501 aacaggatct gttattacca cgattggttt aaccttgatc cctgtcgcta ttggaaatat
    10561 gggaaataac gttccagagc caactggtca aagtctcttg cttgcagcta ttactgttct
    10621 gattatcctc ttgatcaaca tctttaccaa aggatttatc aagtctatct ctattttgat
    10681 tggtctggtt gttggaactg ccattgctgc tactatgggc ttggtggact tctctcctgt
    10741 tgcggtagct ccacttgtcc atgtcccaac tccactctac tttgggatgc caacctttga
    10801 aatctcatct attgtaatga tgtgtatcat cgcaacggtg tctatggttg agtcaactgg
    10861 tgtttatcta gccttgtctg atatcacaaa agatccaatc gacagcacgc gccttcgcaa
    10921 cggttaccgc gcagaaggtt tggcggtact tctcggagga atctttaaca ccttccctta
    10981 caccggattt tcacaaaacg ttggtttggt taaattgtca ggcatcaaaa aacgcctgcc
    11041 aatctactac gcagctggtt tcctggttct ccttggactg cttcctaagt ttggtgccct
    11101 tgcccaaatc attccaagct ccgtcctcgg cggtgccatg ctggtgatgt ttggttttgt
    11161 atctattcaa gggatgcaaa tcctcgcccg agttgacttt gctaacaatg aacacaactt
    11221 ccttatcgca gctgtttcaa tcgctgcagg tgtcggtctc aacaacagta atctctttgt
    11281 cagcatgccg acagccttcc aaatgttctt ctcaaacgga atcgtcgtag ccagcctact
    11341 cgctattgtc ctcaatgccg tattaaatca taaaaagaaa taagaaaaag aggtgcgagc
    11401 ctcttttttg tgtgacaaaa taaaaacatc tacctattca tatatgctag tgtcatagtc
    11461 ctgaaaatca tctgcatcaa gaacaatttc acaactctta tacttttctc ttacaagtcg
    11521 ttcggcttca tctggatttt cagcctctat acttactaaa cgtgataaag tttctgtaat
    11581 ttctactgta tactttttca gttttagatt gctttccaat aattcaccta ttaacaataa
    11641 tctatgatag gagctatgta gccactctgg caatttatct cctacttccc acccatattt
    11701 ttccttcatc aaagctagtt gtttttcgtc accaaatttc atattttcag ataaccatct
    11761 cacacaatcg ggatctgata aggtacagac gattaaactt aggttatcgt aacggctttc
    11821 agtttcataa tcaggccaga tgacttttaa gaaatcatac acctcttctg atgcaacaag
    11881 ttgtttcgtt ttcataattt ccgatacttt cctctacctt taaattctat tatttctttg
    11941 tctcttaata tttgaagctg ttgcctaatc ttttctttga tatgattgtt cttaacaaag
    12001 atatttttta ggtcactttc aaaacgatac atatcttcaa gggtaaattc tgaaccctct
    12061 atcttatcta tacaatttag aatttctatt gtccaacctc ttgatgacag agagctcttc
    12121 cttaaaaata aaccttgctt aaattctttt gtaacttttt ctggatctct aacttgtcca
    12181 tcttgcacaa gaaatatcct tcctttagaa ggtacttgtg ataaatcaat gttacaacca
    12241 atccaacctg ctcgtctagc agttggtgca agtggttttc tttgaataat cgatttcggt
    12301 gtaacaaatt gcttcggaag gacaagaaag ttatttacct caaaattttt tgtgtaagtt
    12361 aaaaaaaaga aattaggatt attatctgcc tgcacacgct tcatcatcgt tgcataagca
    12421 ccatcattga ttgttgatga aaaatttcct tttttgctct ttagttcaaa ctcctcacta
    12481 caatgattac agtaaaaatc tgctacaggc cgattatttt caaaatgatt taagggattg
    12541 ttcccacaat tagggcaata agactgacga taaacccaat cctccgttaa gatacgtgcc
    12601 ttttggctat tacttttata ggtttctact aattctaaat taaagtgtaa ttccatttaa
    12661 aaattcctca ctaatatgag aactcactcg atataagcaa ttaaaatcta ctataactag
    12721 tataccacat atatgtacct attacatttg tatttcatca ctctctttta tatttccttc
    12781 taacatatct cataaattaa aaatgagtga gataaattta tcccactcac ctttctttat
    12841 atacttaccc gtctttcctc agacctttgg cctgtgacaa acatggtaaa ggttgcctta
    12901 cagacatttc tgccctcttg attggtgata tccacatcca tcacacaggt tgtacgacct
    12961 tgatggacac attctccttt aatggtcaat acatcgtcga gttttcctgc cttaaggtag
    13021 ttgatagagg attggagtgt caccccatca agtcccagcg agataaccac caaaccactg
    13081 atttggtcac atagcgtgaa tagatagcca ccatgggcat tgccatagta gttgagcgat
    13141 gagttcacta ctttggtcgt caccacaacg tgaccatctc tcattttttc aatttcgtaa
    13201 ctttcaaagg cagatatagc gtcaaaatga aagtctttca tcgtttcctc ctgatatttc
    13261 aaacgacact atgatactac tttttatagg actgtgcaag gagaaagctc ctagtctagt
    13321 aaaattccga cctgttccac aaagcgcata aaggctgtct gcccttgttc tgtctgcttg
    13381 tagactcctg catcctcaag tacacgcaca aagatagcac ccacagagtc cttgacgatt
    13441 gcaagggctt tttctttatc cgttaggtct ggatgttggg atttgagttg gtctgcccac
    13501 tcctgatgat aatcggcaac tgtaacagct tctcctacaa gatagctagc gacttgctct
    13561 acttcttctt tcagacgtgg tggcaagatt gccaagccca tgacctcaat caagccgata
    13621 ttttccttct tgatatgttg gacatccttg tggggatgat agatgccatc aggatgctct
    13681 gctgaagtct gattgtctcg caagaccaag tccaactcaa actgtccatc acgcttacgc
    13741 gctattggtg tgatggtgtg atgcggtgtc ccgtctgtct ctgctaaaat ctgcacacta
    13801 gaatctgaat actgacgcca ttcctggaaa atcttatcag ctaagttaat caaatcctct
    13861 ttggaatccg aagtcaagcg taggacagac attggccact tgataattcc agccttgacc
    13921 tgctcaaaac cagcaaatcg gaaggtcttt tgcaagggag ccaattccat aggaaatacg
    13981 tgacggcctc cctgataatg atcatgagtt agaatagagc cccccacaat cggcaggtcg
    14041 gcattagatc cagcaaaata tcctggaaac tgctctacga tagccaacag acgttcaaaa
    14101 ctctgacgac taatggccat gggacgatgc tggccatcta aaaagataca atgctcatta
    14161 aagtaagcat agggcgaata ctggaacccc cattcctgac caaccatttc aaaacggata
    14221 atccggtggt tgctgcgagc tgggtggttg acccgaccat ggtaaccctc attctctaaa
    14281 caaagttgac actgaggata attactagct tgcaccaact tggctaccgc aatctctttc
    14341 ggatcctttt caggcttaga gagattgatg gtaatttcaa gttctccata atcagatgga
    14401 acacgataag cgatattttt agcaatggcc ttgagtttga tgtaatcatt tttctgactg
    14461 agttgataaa aatcctctat cgcttgctca ggagagtgga cataggcttc ccagaagtca
    14521 cgattgacct gactcggata aggagtcacc aagtccatca gttcagtacc aaggatttca
    14581 cgcgcagtct gactatcctc aatcatctct aatcgaacgg cttcctcaac tagctggtcc
    14641 ttgaggtcaa tcaatttatc cagattggtc tcaacttcca aaacaccttc tcccactcgt
    14701 gccaaaacac ggttggtcag gtagattcga tccatttcct caaatgaact ttcagaaatg
    14761 acatgtgtta caaatttatc tactaagatc actatagagc ctccttttga ctagtcaagg
    14821 acgcgagtgc cacctgcaac ttcagcgata tagaagcttg gagcgtatcc aactacttcc
    14881 tcgtagtgtt tgcctacagc ttccttaaag gcctcaacag tatctttttg aaccaaggca
    14941 atggcacagc caccaaaacc agcccctgtc atacgagcac cgagaactcc ttcttgtgcc
    15001 caagctgtgt gaacaagagt atccaattcc aaaccagtta cttcataatc atgctccaga
    15061 gaaacgtgtg acgcattcat caaacgacca aatgtttcca aatcacctgc ttgaagggct
    15121 gcttgagctt taagggtacg ttggttttca agcacagcat ggcgagcacg tttcaaacga
    15181 ttttcatctt taatcaaata gctatattgg tcaacggccc actcgtccaa ttcacccagg
    15241 gtctgaatat ccaaggcaac ttgcaattct tccactgctt tttcacactc agcacgacgt
    15301 tcattgtatt tagagtccgc caattcacgg cgtttgttgg tgttcatgat aacaacgaca
    15361 ttgtccttca aatcaagtgg caccaagtcg tattctaaag tattggtatc taagtaaata
    15421 gcacgttggt cagcccccat accaatagca aactggtcca tgataccaga gttgactccg
    15481 ataaagttgt tttctgtttg ttttccgatt ttaaccaaat ccaaacggtc tagttttaaa
    15541 tcaaagagat gctctgccac gactcctgtc aagagctcca aggatgctga agaagacaag
    15601 ccagcaccat ttgggatatt cccataaaca taaaaatcaa aacctttgtc aatcacgtgc
    15661 ccagcttctt gcaagaaatg aagaacccct tttggataat tggtccagtt gtgctctttt
    15721 tcaaacttga ggtcagcgag aggcacttcg ataatgccct tgtcctcaaa gttagctgag
    15781 tagaaacgca agacttggtc gtcacgctta cgagctgcac cgtaagttcc caaggaaata
    15841 gcagcaggaa aaacgtgccc accgttgtag tctgtgtgtt caccaatcaa attaatacgg
    15901 cctggtgaaa agaaggtttg gtctgcttct tgaccaaaaa cagcaagaaa gtctttgcga
    15961 aggacttcag tagtaagatg ttgtgccata tgaattctcc tttgactgtc cgttaagcca
    16021 tcttaacgtg taatcgtttt ctttctattg tatccaagga atttcccaag gtcaatcctt
    16081 tttactaaaa ttttactaaa atatcagtaa aaagaagaaa aatatgatat actaacctaa
    16141 tcaaggagga tttatggcta ccttaaaaga cattgcacag ctagcctctg tctcaatcgc
    16201 gaccgtatcc cgtgtcctca accgcgacca gagcctatct gttacagaag aaaccagaca
    16261 ccgtatttta accgttgctg aagagctggg ctacaccaag cacctcaaga caggcgagtc
    16321 ccacaaaccc aagcaaaaga ttgccattat tcaatgggtc agcgaacaag gggagctaga
    16381 cgacctctac tactaccaga ttcgcctagg aatagaaaaa agagcccaag agttggacta
    16441 cgatatcttg cgctatttta atgaccaccc ttttacccta agcgaggaag tgattgggat
    16501 tctctgcatc ggaaagttta gtcgagctca gatttctgcc tttgaagaat accaaaagcc
    16561 tcttgtattt ctagacagcg atacactttc cctgggacat acctgtatta tcacggattt
    16621 ttacactgct atgaaacagg ttgtcgatta tttcctcagt caaggaatgg accgtatcgg
    16681 gattctaaca ggccttgaag aaacaacaga ccaagaagaa atcattgagg ataagcggct
    16741 ggaaaatttc agaaactaca gtcaagcaaa aggaatctat catgatgaac tgatctttca
    16801 aggaaacttt actgcccagt ctggctatga cttgatgaag gaggccattc agggcttggg
    16861 agaccaactt ccgccagcat ttttcgcagc cagcgatagt ttagctatcg gtgccctccg
    16921 tgccctccaa gaagctggaa tcaacctgcc agatcgcgtc agcctcattt cctttaacga
    16981 cactagtctg accaaacagg tctatcctcc cctctctagt attacagttt atactgaaga
    17041 aatgggccga gcaggtatgg atattcttaa caaggaagtc ctccacggtc ggaaaatccc
    17101 tagcctgacc atgctgggaa ccagactgac attaagagaa agtaccctaa atcaagaata
    17161 ggataacata aaaaacgaat aggagtttta gaactcctat tcgtttttta ttcgattata
    17221 atcatagact taatggtctt acgttcatcc atatctttgt aggcttggtc gatatcttcc
    17281 agtttataac ttgaagtaaa gacgcgacct ggattgatat caccatcaag gacggctttt
    17341 agtaaaaatt gcttatcgta tgttgtagca gaagctgccc cacctgctac agagatattt
    17401 tgcataaatg tcgaaccaag agcacgatta ttatagtgtg ggactcctac aaagcccata
    17461 cgccctccat tatgaagaac acctagcgcc tgttctatag cagcctccgt accaacacat
    17521 tcaagtgctg catctgctcc tccaccgagg atttcacgca ccttggtaat tccttcttga
    17581 ccacgttctg caacaacagc tgtcgcacct gactccatag ccatcttttg acggtcttca
    17641 tgacggctca taaggataat ttgtgatgct ccacgcatct tagccgcgat gacagcacat
    17701 tgaccaacag ccccatcacc gataacaaca accttgtccc ctttttgaac atttgcaaca
    17761 cgcgctgcat gatagcctgt cggcatgaca tctgcaagag tcaaaaggga cttgagcatc
    17821 ccttctgtat aatcagaagg ttgaccaggg attttaacca gcgcccagtt tgcatagtgg
    17881 aagcgaatat attctgcctg aaaatcaccc cccaaattat tgccaatatg attgtcgcaa
    17941 gaaccgtcaa atccagcaag acaggcatca cactcaccac atccatgtgt aaaagggaca
    18001 atcacaaaat cacctggttt caccgtcgta atggcttccc cagcttcttc aacaatccca
    18061 atcgcttcgt gtccactatt tttatgtcca gctttcgttt ctggattacg gtacctccat
    18121 aaatctgaac cacaaacgca cgcacgaacc acacgaataa tcacatcatc cgcttctatt
    18181 atttgcggac gttcaatgct agcaagtcca acctgacctg cctttgtata tactgctgat
    18241 ttcatttaaa attttccttc cttataaagt ttaattttga gatttaaacg atttaaagct
    18301 gtctgtagct gagacaagcg ctcctctaat ttttgctttt cctcttctaa aataccaagc
    18361 ctctcctctc tcgtttcatc tcccttttgg tagagcgaca tatagtcaac taaactatct
    18421 acagagacac ccgccgaacg aaaacactta ataaattcca gcgcttcgat atcctgatct
    18481 tgaaaatcac gaatcccagt agcagtacga gtaatcggtg gcacaagacc aacccgttca
    18541 taataccgaa tcgtatccgc tgaaattccc aacaaatcac tggcagattt aatattcaca
    18601 cgctaacctc ctctcatttc attcactgaa ttcacgctat tctaactcac ttatcttatt
    18661 atataacttt gagttgactc caagtcaagc attttgtatc taatcgaaaa atttttatca
    18721 ttaacaatca tatatccgga gtttatacaa tgctcttctc ttaccaattt ttgaaatatt
    18781 taaataataa agaaattact ctattccaaa taagatagta cccaagtatt tttcactttt
    18841 ttctaatgtt gatgctcata actccgttcc aagtcacaca cctttcgctt atgggtttgg
    18901 tgagtttcta ggtcagcatc aatttcaatg gtaatatttt gaaaaccaca atcttttagg
    18961 aaaattcgaa tagactcttt acaagtttcc atatgttcca tttcttttag acaaacatgg
    19021 acaatggcat ttttttccaa agcatccata gtccagagat taagctgatt aaggctggcc
    19081 acattgtcca atcgctccag gccactcttt acttgcttga tatcaagacc ttctggcaca
    19141 gcatccaaga aaatcttgag tgtagaccaa aaacgtggaa gggcttttga aagaataaag
    19201 aaagaaatga caagggacaa aagaggatct aggatatacc agtccgtaaa tcgaagaaca
    19261 atcgccatca ggataacagc tacccaccct agcgtatctt ccagaaaatg caaactcaga
    19321 atagactcat tctttgtctt tcccttacca accaccagac tcgctaacag attgatagta
    19381 atcgcaataa ttcctaacca gagaatcccc tcatcattga ctggttgcgg atgcaaaatc
    19441 ttcgtgacat tttccaaaat gactagaaca gagcccgtta cgagaatcac agctgttacc
    19501 aaggctccta gcaggctaaa ccgcttatag cccaaggtgt actgattgtc ttcttcacga
    19561 ttggagattg tttctagaaa agctgatatt ccaattgcaa tcgcatctcc caagtcatgc
    19621 acagagtcag caagaacagc gctagaacca aatactccac ctgcaataaa ctcaacaatg
    19681 gcataagtca aatttaagaa aaaagccacc caaacagcat attttgcctt catatttctc
    19741 attcctttgt tataatagat ttatgaacac cttgttcatt atcattatcc actaaaactc
    19801 aaagaaagga tagaagcaaa acgtcagctt tattcagttc tgaacaattt gccttaagtg
    19861 tccatatgac taacattgac cgccgtatca gcaaaaccaa aaaagccatc tatcaagctt
    19921 ttatacaact tttgaatgct aagggctacg aggccactac tgttcaggat atcattgatc
    19981 tcgcagatgt gggaagatcc accttttact gtcactatga aagtaaggag cttcttctgg
    20041 accagctctg ccgctacctc ttccatcatc tttttgaaag agagcaagcc atttcaaccg
    20101 aggactacct cgcccacctc tttctccatt ttcataaaaa ccaagaccat atcaccagtc
    20161 tgctattttc caaaaatgac tacttcctcc gtcaactcca taaagagcta gaacaccatg
    20221 tctattccgt gctagctgat aatttaaaag aagcccaccc taatatgcct acttcttacc
    20281 tccaacattt tgtcgtaact aactttatcg aaacattgac ctggtggctc aaaaaaggtc
    20341 aagatttcac agaccaggaa gttgtccaat tttatctaga ccttctcatt cctaaaaatt
    20401 gaatatagag taaaacttca gttgtcttat ttctaggtta ctgagttttt tatcttttca
    20461 acaacaaaag aggacccgcc gatcctcttt ttcatactat aaatccttga ttatcaacta
    20521 tatctgtttt aatcgaaatc tcaaaacagc actttcaaac atcttttcct agttaagtaa
    20581 atcagtattc tgcttagctg ccttgctcca ttgataccaa ccaactagac tgttaatgag
    20641 ataaattaga tatttccctt gaatttgcag gctttctccc caccagagat agattgaaaa
    20701 gacattggta gccgcccaga atatccactg ttcacggtaa acagctgtca tgaggatttg
    20761 ccctacccca ttggttgcat ctgtgattga atcacgatag ggacgattgg caccaataga
    20821 ctgataaatg aagccaaagg ccaaccacca aagcacacta atggaaagat actttgtcca
    20881 gcccttgccg tccagtttac gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg
    20941 ataaatccaa actagaagtc caattggctg catgactgtg aagtaaagtg tcgtcagcac
    21001 ctcaccataa aagcctttct gtagggccaa aataaggtaa ataacagagt taatcaagcc
    21061 aaaaagataa ttacttgctc gaccttccga tacaaagatt acacagataa tccctgtcaa
    21121 gctacaaatc atcccaatcc agtcaacaat acgatgttcg taaaccaact ccagccagag
    21181 aggaaaactt cctaaaacca gcaaataaat ccactgggca aaactacgat gggcaaagag
    21241 gtcatcccag atagccttca tagttcctga aaatcctaaa tcagccatag ccgcaaccat
    21301 acgacggtaa ccacctgaca tttcacctag ggttgttttg atattttcaa ttttcttttg
    21361 caaataagta tgcatcattt ctccttttgt ttttaaagag ccgtgtctgg atagactttc
    21421 ggacgcaacg ctctattaga taatgaactg cctatacaca agatttctaa ccttagtcga
    21481 catgagctga aacctcttat ttgttaagta gttcacaaaa tattatacac ctattttatg
    21541 aatagtcaac tgtctttaca gtaaaatttt agaaaatcat gaaaattttc tctttctttc
    21601 cattttaagt gacattcagt cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc
    21661 attcttttat ctagtcgttt aaggaagttg agttcagtat gtttaaagtc tctgtcccat
    21721 catttcttca acaaaccttg ttcttggaga aactccttgg ctacttgctt tgctgacttg
    21781 ccttcaacac cgacttggta gttgagctgg ctcatctggc tttctgtaat cttaccagcc
    21841 aatgtattaa gaactctttc caactctggg tgtttcttga gaagagcttc tttcatgagt
    21901 ggagcccctt gataaggtgg gaagagttgc ttgtcatctt ccaagacctg taaatcataa
    21961 cgctccaatt ccgcatcagt cgaataggca tccgtgattt gaatatcccc tgactgaatt
    22021 gcctgataac gaagagctgg ctgcatagtc gccacattga gattgagacc atacattgat
    22081 tgcaagccct tatttccatc ttcacggtcg ttaaactcaa gcgtaaagcc tgccttcagc
    22141 tgcccttcca cttttttcaa atccgaaatg gtcttcaagc catattcttg agcaatcttt
    22201 ttcggaacag ctacagcata ggtattttga taagacatag gtttgagata ggctagatga
    22261 tcctgcttgg caatgccatc acgcgccact tgataaacct gctctggctc atgactcacc
    22321 ttgggtgatg gttgaagcaa actttcagtc accgtaccgg taaattcagg ataaatgtca
    22381 atatcacctt ttttcagagc ttcataaagg aaacttgtcg tcccaaaatt cggtttaaca
    22441 gtcgcagtca tgctggtatt ttcttcaatc agcaatttat acatattagc caaaatttct
    22501 ggttctgggc ctattttccc agcaataatc aagttttcct tctctttttg agccaaaagg
    22561 gctggactat aagacagacc cagtaataaa gccaccaagg caaaaccaga gaaaatcgtc
    22621 cgcaatttcg ctttttccat tacttttagt aggaagttaa aggcaatagc tagcactgca
    22681 gaagaaaggg ccccaatcaa aatcaaactg gcattattac ggtcaattcc caacaggata
    22741 aaggaaccca gtccccctgc cccaatcaag gtcgccaagg tagccgtacc gataatcaag
    22801 accgctgccg tccgaattcc agacatgata acaggcatgg caagtggaat ttcaaacttc
    22861 ttgagacgct cccatctggt catcccaaag gcaatcccag cctcttgaag actcggatca
    22921 attcccttca gcccagtaat agtattttgt aaaatcggaa aaatcgcata aatcactaga
    22981 gctgtcaatg ccggtaaagt cccaattccc atcaagggaa taaagagtcc caacaaggcc
    23041 agagacggga tggtctggaa aatccctgca atctgtaaga cccaatccgc caacttctca
    23101 tgatagcgaa gataaacagc caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa
    23161 agtgacaact gcaaatgttg agatagggct gtcaaccaat cactaaaacg atcctgaaaa
    23221 gttgcaatta aattagtcat gaacactacc tccaaacaag tctgctacaa agtctgttgc
    23281 aggcactttt aaaattgtct cgggattcgc tacctggcga atttctccat cctgcaagac
    23341 agcaatacgg tccgccaact tcaaggcttc atccgtatca tgggttacaa aaatcgttgt
    23401 catcccaaac tctttatgca attcttttgt cagaacctgc aactgttttc tcgaaatagc
    23461 atccaaggcc gaaaagggtt catccatgag gaaaatcttg ggctgaccaa tcatagctcg
    23521 gacaataccg acccgttgct gttctccacc agatagctca cttggaagtc gatgcccata
    23581 ctcagctact ggtaatccaa cttttgccaa aagctcttct gttttttgag caatttcttc
    23641 cttagtccaa cccttcattt caggaatgag agcaatattt tcctcaactg ttagattggg
    23701 aaagagagca atagcctgta aaacatatcc agtagtaaga cgaagttcac gctcatcata
    23761 gtctttgatg cgcttgccat ccatataaat atttccatca gttggttcca agagacggtt
    23821 aatcatctta atcatggtcg tcttacctga cccagaaggc ccaactaaaa ccatgaactc
    23881 cccattctca atctgtaaat tgacatctct caagacatct ttttctgtgt agcgcagtgc
    23941 tacatttttg tattcaatca ttctttgtcc tcaatttaaa acttccctcg attagtcaag
    24001 tcttctacct taggtatgac ttccttatta tcccaatgct ccacaatttt cccgttctct
    24061 aaacggaaga tatcgtactg ggcataagca acgccatcaa tctgagtctg accatagcta
    24121 accacatagt ttccttgtcc taagagttgg aaaacaaagt caaaagtgac actatattca
    24181 gccacatagt ttttataagc agcacttcct tgtccaatct catgattatg ctgaatcaaa
    24241 tcgtctgcca cataatcact ccactgctct agctccccat tttggaaagt ttctgtcaag
    24301 aaacggcgaa ccagcttttt attttctgct ttcttgtcca aatccttgat ttcaaaatct
    24361 ccaaaaattt gatctagttg gtcattttca ggtgttcgat agtagtcaat gacatcccaa
    24421 tgctcaacaa tacgaccatt ctcatccgca cggaaagtat ccgtcgtcac ccattgagct
    24481 tctccaccat tcagatattg atgaacatga acaaagacca gattgccatc ctcaatggtg
    24541 cggacaatct taatctgacg ctctggatga cgctcaaaga aatctgcaaa gaaggctgca
    24601 aatccttctt tcccgtcagg aacacctgtc gaatgttgga tataggtatc ccctacagac
    24661 tgggcttgag cctcagcaac tcgtccgtct tgaatggcat ggatgtatag gttgtgagca
    24721 tttttcactt gttgtgacat attctaaacc tcattttcct tctctttcag atttgccaaa
    24781 attctttctt gaaaaccttc aaattggtga atttcttcct ctgaaaatcc tttgtaaaag
    24841 atagtatcca atttctgact gacacgatgc cccacttctt tctggaactt gcctaactcc
    24901 gttaaaacta aatacttctt acgcttgtct tttccacacg gactaacaat tacaagcttt
    24961 tgttcctcta gcttttttat catagtcgtc agcgtattat tcgcaagccc agtcgcaagc
    25021 gcgatatctg tcgcagttgc gcagccagtt tcactattcc ataaaaccgc taaaatcttg
    25081 ccctgttcac ccctataaag agcctcagga tcttgactca gtaacttttg aaaaatccgc
    25141 ccattcaaca aacgaatatg atgggctagc aaatgactat ctttcataac acctccaatt
    25201 tatttcgata tcgaaatgaa taaaataatt gtaacactca tcgttctaac tgtcaactat
    25261 ttcgatttgg aaataatttt tgataattat ccacaccacc atactccggc tcaactaact
    25321 tttaacgaga gtttctaaac tccttcgtcc tccagtctac aaaagccttc cattcgtact
    25381 atcctatatt ttatgagggg acacattttt cctatcagac catttatttt aaagatagaa
    25441 gtaaatcata attgcttcca tctgttcttt tatagtatat tgaagttaga ctagagcact
    25501 gtatcttcta aaacattgat agaaagcgat ttgaatttcc caatcaattt gttcgtattt
    25561 atagcatttc gaaactggaa taggacacca tgactgctaa aagatttcta taaattcatt
    25621 tgattttcct aatcaatttg ttcatatctt atttcattcc gctataattt caccttaccc
    25681 tatctttttc gtagcaccct tcaaacagcc tatcccctac cgtttgacga ttcctcactt
    25741 cactccactt ccattacaga agtttcttca ctactatggt ctcggctgac ttctcatgat
    25801 tccttgttac tactatttga acgctcacga gataagtctt ataaaaaatg ctttgatcca
    25861 caatggaatc aaagcatttt aaaaagttcc tcatacataa gcgcagaagt cgcagttcct
    25921 ctgtacttgg cttcttctct tttgacaaag cgagccaagt tgagcaactc aggtgctgga
    25981 tgtttgggat ttaggagcaa ttcacgattg accaggcctg agagacgaac tgcctgcaat
    26041 tgctcatttg tagtaggcag ttttttagta gtctctagga gagcagcaac taaatcttca
    26101 ctcaaatcat gtcgagcatg attgtaaaga tcttttataa ggctttctag gtttggttct
    26161 accatcccta ccacctccct tatggtttaa taatgtttaa tcaaatcaac cgttgaacga
    26221 tccaatttct tcaccaaggc ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca
    26281 tagagggttt ggtgagaatg gatataacga gcgcagacac cgatagttgt tgatgggaca
    26341 ccaccatttt tcagatgagc tgcacctgca tctgttccgc ctttaccaca gtagtattgg
    26401 tacttgatac cagcttcttc agccgttgtc aaaaggaaat ccttcatccc tgggagaagc
    26461 aagtgacctg gatcatagaa acgaatcaag gttccatctc caatcttgcc ttgaccaccg
    26521 tagacatcac ctgctggtga gcaatcaact gcgaggaaga cttctgggtc aaacttggtt
    26581 gtagaggtat gagcgccacg cagaccaact tcttcttgga cgttagaacc cagatagagt
    26641 tcattgccga gtttttgacc cgataaagct tcagctagct cgcttaccat gaggacaccg
    26701 tagcggttat cccaagcttt tgagatgata tttttttcat tggctgtcaa aattgcagaa
    26761 ctatctggta caatggtatc accaggacgg atgccaaaac tttctgcctc agccttgtcc
    26821 gcaaaaccac catcaaaaac gatatcggca atggctggca tggttggtcc cccctttcca
    26881 cgagtcaaat gcggaggaac agaacctgaa atcacaggaa tttcatgacc atcacgagtc
    26941 aagagtttga aacgttggct gctaaccacc atggggttcc agccaccgat ttctacgaca
    27001 cggaaggtac catctggctt gatttcgctg accataaaac caacttcgtc catatgagaa
    27061 gcgaccaaga cgcgcggtgc atccacagct tctgaatgtt tgataccaaa aataccaccc
    27121 aagccatctg tcaccacttc atccacatgc ggtgtcaact tttcacgaag ataagcacgg
    27181 acaggcgctt catgacctga gactgcagca agttctgtta cttctttaat ttttgaaaat
    27241 aatgttgtca tttcagttcc ttctttcttt catccatttt accacttttt ataggagaag
    27301 gatagtggga aggtggattt ctaagttagt atcttagtcc tgctctatct tagaaaagga
    27361 tagtattctc ttgcatgtag tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt
    27421 tatttccaaa caaaaaaaca atacaccatc aaagttgttt ggatttttca tgaaatttac
    27481 agaaaatagt tgacttccct ttcttctttc tttaaatata tagttggttg agtttggaat
//
LOCUS       DAVLFB010000018        26821 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLFB010000018 DAVLFB010000000
VERSION     DAVLFB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.018"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            135..683
                     /locus_tag="VXD58_000683"
     CDS             135..683
                     /locus_tag="VXD58_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV0675425.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            793..1692
                     /locus_tag="VXD58_000684"
     CDS             793..1692
                     /locus_tag="VXD58_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV0675426.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1717..2991)
                     /gene="serS"
                     /locus_tag="VXD58_000685"
     CDS             complement(1717..2991)
                     /gene="serS"
                     /locus_tag="VXD58_000685"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV0675427.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVEAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3205..3576)
                     /locus_tag="VXD58_000686"
     CDS             complement(3205..3576)
                     /locus_tag="VXD58_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV0675428.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3579..4943)
                     /locus_tag="VXD58_000687"
     CDS             complement(3579..4943)
                     /locus_tag="VXD58_000687"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV0675429.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5312..6097
                     /locus_tag="VXD58_000688"
     CDS             5312..6097
                     /locus_tag="VXD58_000688"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV0675430.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6191..6625
                     /locus_tag="VXD58_000689"
     CDS             6191..6625
                     /locus_tag="VXD58_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0675431.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6625..7599
                     /locus_tag="VXD58_000690"
     CDS             6625..7599
                     /locus_tag="VXD58_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV0675432.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7659..7883
                     /locus_tag="VXD58_000691"
     CDS             7659..7883
                     /locus_tag="VXD58_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0675433.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            8002..8976
                     /gene="fabK"
                     /locus_tag="VXD58_000692"
     CDS             8002..8976
                     /gene="fabK"
                     /locus_tag="VXD58_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV0675434.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8969..9889
                     /gene="fabD"
                     /locus_tag="VXD58_000693"
     CDS             8969..9889
                     /gene="fabD"
                     /locus_tag="VXD58_000693"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV0675435.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTSLLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTTHLAHVEDQASLVALLEK"
     gene            9923..10654
                     /gene="fabG"
                     /locus_tag="VXD58_000694"
     CDS             9923..10654
                     /gene="fabG"
                     /locus_tag="VXD58_000694"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV0675436.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10676..11911
                     /gene="fabF"
                     /locus_tag="VXD58_000695"
     CDS             10676..11911
                     /gene="fabF"
                     /locus_tag="VXD58_000695"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV0675437.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11914..12399
                     /locus_tag="VXD58_000696"
     CDS             11914..12399
                     /locus_tag="VXD58_000696"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV0675438.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12396..12818
                     /gene="fabZ"
                     /locus_tag="VXD58_000697"
     CDS             12396..12818
                     /gene="fabZ"
                     /locus_tag="VXD58_000697"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV0675439.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12830..14197
                     /gene="accC"
                     /locus_tag="VXD58_000698"
     CDS             12830..14197
                     /gene="accC"
                     /locus_tag="VXD58_000698"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV0675440.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14234..15100
                     /locus_tag="VXD58_000699"
     CDS             14234..15100
                     /locus_tag="VXD58_000699"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV0675441.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15097..15864
                     /locus_tag="VXD58_000700"
     CDS             15097..15864
                     /locus_tag="VXD58_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV0675442.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            16023..16208
                     /gene="briC"
                     /locus_tag="VXD58_000701"
     CDS             16023..16208
                     /gene="briC"
                     /locus_tag="VXD58_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV0675443.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            16699..16926
                     /locus_tag="VXD58_000702"
     CDS             16699..16926
                     /locus_tag="VXD58_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0675444.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16975..17397)
                     /gene="nusB"
                     /locus_tag="VXD58_000703"
     CDS             complement(16975..17397)
                     /gene="nusB"
                     /locus_tag="VXD58_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV0675445.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     AVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17390..17779)
                     /locus_tag="VXD58_000704"
     CDS             complement(17390..17779)
                     /locus_tag="VXD58_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0675446.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17801..18361)
                     /gene="efp"
                     /locus_tag="VXD58_000705"
     CDS             complement(17801..18361)
                     /gene="efp"
                     /locus_tag="VXD58_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV0675447.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18490..19932)
                     /gene="gatB"
                     /locus_tag="VXD58_000706"
     CDS             complement(18490..19932)
                     /gene="gatB"
                     /locus_tag="VXD58_000706"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV0675448.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19932..21398)
                     /gene="gatA"
                     /locus_tag="VXD58_000707"
     CDS             complement(19932..21398)
                     /gene="gatA"
                     /locus_tag="VXD58_000707"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV0675449.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(21398..21700)
                     /gene="gatC"
                     /locus_tag="VXD58_000708"
     CDS             complement(21398..21700)
                     /gene="gatC"
                     /locus_tag="VXD58_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV0675450.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            21913..23457
                     /locus_tag="VXD58_000709"
     CDS             21913..23457
                     /locus_tag="VXD58_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV0675451.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23726..24211
                     /locus_tag="VXD58_000710"
     CDS             23726..24211
                     /locus_tag="VXD58_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV0675452.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            24327..24515
                     /locus_tag="VXD58_000711"
     CDS             24327..24515
                     /locus_tag="VXD58_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV0675453.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24672..25037
                     /locus_tag="VXD58_000712"
     CDS             24672..25037
                     /locus_tag="VXD58_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0675454.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            25040..26707
                     /locus_tag="VXD58_000713"
     CDS             25040..26707
                     /locus_tag="VXD58_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV0675455.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7709 a         5086 c         6204 g         7822 t
ORIGIN      
        1 ggaagtgtga taaactaata taacaacgaa aatcttatga gagaataaag ctttatctta
       61 gataaactta ggaatcagga ttttataaga ttttccaata atattagtgt caataataag
      121 aaaagaggta tcttatgaca acatttacta tccatacagt agagtcagca ccagcagaag
      181 tgaaagaaat tcttgaaaca gtagaaaaag acaacaatgg ctatattccc aacctaatcg
      241 gtctcttggc caatgccccg actgttttag aagcctacca aattgtctca tctatccacc
      301 gtcgcaacag cctgacaccc gttgagcgtg aagtggtgca aatcacggca gccgtgacca
      361 atggttgtgc cttctgtgtc gcaggtcaca cagccttttc catcaaacaa atccagatga
      421 atgatgactt gattcaagct cttcgcaatc gtactccaat tgaaacagat cctaaattgg
      481 ataccctagc taagtttacc ttggcagtta tcaataccaa gggtcgtgta ggagatgaag
      541 ccttgtctga gtttttagaa gctggctaca ctcaacaaaa tgccttggat gtggtttttg
      601 gtgtcagcct agcaatcctc tgtaactatg ccaacaactt agctaataca ccaattaatc
      661 cagaattgca accttatgcc taattcatat ctgagtaaaa tgaagtctga aataagcgga
      721 cttcgttttt ttaaatcctc atttaagcgc ttttatgtta tactgaaact aacatgatta
      781 ttggaggtta ggatgaaaaa actcccctta gtattttcag gttgtttgct aggtttggca
      841 ggagctggaa atcttatttt agatacgttg ccggttctat cccatctttt tagtctgact
      901 ggtttggttt tatggattta ctttctaatt ctgcatctct ttaattggaa agaaaccaag
      961 caagaattga ccaagccccc tcttttgtca ggaatggcaa cctttcctat ggctgggatg
     1021 attttatcga cctatgtctt tcgcgtattc tcttatcttc ctttggtagc acaagggatt
     1081 tggtggtttt catttctctt ggatttgacc ttgattgctg gttttaccat caagtttgct
     1141 tgttcagggc ggagggttca tgccactcca agttggacgg ttctctatgt ggggatagca
     1201 gtggctgcct tgacctatcc tctggtaggt attatcgaaa ttgcctatgc gaccttgagt
     1261 tttggttttc tcttgacctt ctatctctat ccccttattt atagcgattt aaagaaacat
     1321 ccactcccac tagccttgct tggacaagaa ggaatctact gtgctccttt ctctctactc
     1381 ttggcttctc tagttcgagt aggaggaacc agcctgccga cttgggtctt gattgtcatg
     1441 attttggctt ctcaatcctt ctttttcttt gttttaactc gtctgcccaa cattttaaaa
     1501 caaggttttc aaccagcctt ctcagccctc accttcccaa ccattatcac agcgacctcg
     1561 ctcaagatgg ctcagggaat tttgaaactt ccatttctgg attacctggt attggctgaa
     1621 accattatat gcctaactat tttattcttt gtactaggtg cttatctgat ttggttacga
     1681 aaaaaggtct agctagaaat agctaaacct tattttttat ggtttgatga cttcagctcc
     1741 acccatgtat ggacgaagtg cttctgggat ggtcacagaa ccatcttcat tttggtaatt
     1801 ttcaagaatt gcagccactg tacgtccaac tgcaagtcca gaaccgttca aggtatgaag
     1861 gagtttcacc ttgccatctg cttcatcacg gtaacggatt tgggcacgac gggcttggaa
     1921 atcttctgtg tttgaacagc ttgagatttc acggtaattg ttttgtgctg gaatccacac
     1981 ttccaagtcg taagtcttcg cagctgagaa gcccatatct ccagtagaga gagcaacgac
     2041 acggtatgga aggttgagtt tttgaagaat gttttcagcg ttggctgtca ttttttccaa
     2101 ttcttcgtaa gattcttctg gtttggcaaa tttgaccatt tcaaccttgt ggaattggtg
     2161 caaacggatc aagccacgcg tatcacgacc ggcagaacca gcctcagaac ggaatgacgg
     2221 actcatggca gtgaagtaga ttggaagatc tttgccgtct aagatttcat cacggtagta
     2281 gtttgtcaga ggaacttcag cagttggaat caagacaaag ttggtatcgc tgagttcaaa
     2341 agtatcttcc ttaaattttg gatactgacc agtaccaaac atagaatcat ggttgactat
     2401 gtaaggtgtg atgacttcag tatagccttc ttttccatgt tcatccaaca taaagttgta
     2461 gatagcacgt tccaaacgag caccgaggcc tttatagaag aggaagcgag cgcctgttac
     2521 cttaccaccg cgttcccagt caaggatacc aaggtcttca ccgagatccc agtgagcttt
     2581 aggttcgaag tcaaactcgc gtggagtacc ccaacggcga acttccacat tgtcgtcttc
     2641 gtcagtccca acaggaacgc tgtcagctgg gatatttgga agagtcgttg taaattctgt
     2701 caatttagca tcgatttctg ccaattcagc atccaaggct ttaacctcag cagatagatt
     2761 ttgcatggca gcaatcttgt catctgtatt ttccttgttg cgcttagctt gggcaatctc
     2821 agcagaaact gtgttacgtt ctgctttgag agtttcaacc ttgaccaaga tgttacgacg
     2881 tttagcatcg atttctttca tttcattcaa gacagcagcc tctacaccac gtgtagctaa
     2941 tttttctgcg acagcttcaa aatctgtacg aatacgtttg atatctaaca taagaactcc
     3001 tttatgaaaa aagcacgcct gacaaaacgt tggagtggca gggccacggt tccatccaac
     3061 ttcacaggtg tgcacttgat tgtgtatgta attgttacta acggtagaat ttcacctatc
     3121 ccccctatct gctcgcagta cccgcagact ttctgaaaga agaagataac ctacttatcc
     3181 gttgctatga ttatactaaa gtttctactt ttttgcaaat agatttttaa atttttggct
     3241 aattgtctga atcagggtcg gaagtttgac gaccttgtca ttgcctagtt tttcgcgtgc
     3301 aattttgaga atggcacctg agtcttttga agcaaagagg aattttcctt tgtctgtaaa
     3361 gacttcgaag tggcggctga ttttgcgtcc agtgacattg gctccaatct gattgatatg
     3421 gctccaagga atctggataa attgttcgac attgacatct gggtaaaatt ccaaagcctg
     3481 atctccgaca aggaatttcc caactttccc agcgatagag aggtaggaag tgcctgtcgt
     3541 actgaggagt actgttttgt taagtgattg ggccatgctt agtcttcctt actttctcca
     3601 aaaaaggcat tgtagagggc tttaattgct gctttctctt ggtctttatt gacaacaaac
     3661 ataatagaaa cttcactaga accttgagac atcatctgga tgttgatttt gttttcagat
     3721 agagcgcgtg tcgcagtagc agtcactccg atgtggctct tcattttttc accaacaatc
     3781 ataatgatag aaaggtcgtg ttcgatttct gcatgatcta ctttagcctt ttgaaccaac
     3841 tgacgcagga tttcttcttc cttgatggga gttagttggc gagaacggag aatgatagaa
     3901 agatcgtcga tacctgttgg catatgttcc caaccgatgt taagttcttc caggatttgc
     3961 agaaccttgc ggccaaatcc aacctcacgg ttcatgaggt atttcgacat gttaatgctg
     4021 acaaagcctg agtcaccagc aattcccaca actggaaatt catcattact gtgttttaga
     4081 acgatacgag tacctggatg gtcagggttg ttggtattct tgataaccag aggaattttt
     4141 ccacggtagg caggaagaag agcctcgtca tgaaggactg agaagcctgc ataggccaac
     4201 tcgcgcattt cacggtaggt caactcagga atcgagtgtg gttggtggat aataccaggg
     4261 tgggctgcaa agataccatc aacgtccgta aagttttcat agaggtcggc tttgacacca
     4321 gcagcaatga tagaacctgt aatatcagaa cctccacgtg agaaagtaca gatttgattt
     4381 tccttagtga caccaaagaa accaggaatg acaaggactt cattggtgtt tgtcaattct
     4441 tcaatcttgt cataacttga tggaatgatg cgagcgtgac caggttcact tgtgaccaca
     4501 atcccagctt ctctagggtg catatagcgt gcatcgatac cattttggtt aaagtaggca
     4561 gcaatcaatt tggcattgtt attttcaccg gctgctagga aagtatcgta gagaaattca
     4621 ttttcttcaa taggaagagt ggccaaggcg tgaatgcttt tagaaatttt ttctagcaca
     4681 gctggtttta gtcccaattc actaaccata gcagcatagc ggtcgataat ccagctttgg
     4741 ctcttgctaa tatcgttacc cgcaacatag tcgcggtagt atttaatcag ggcatccgta
     4801 accttagtat cttcagcatt gcgtttacca ggcgcagaaa cgactacaaa acgacgctct
     4861 gaatcgcttt tgacgatgtt taaaactttt tctaattgac tagcagaggc aagagaacta
     4921 cctccaaatt taacaacttt cataagaact cctaaagtaa gtattttata cgattatagc
     4981 agaaagaaag gcttttttca atgaagaaaa taagatgctt tctagcctaa agcgagcctt
     5041 tcttatcggc tgagacactt tcagtcgatt ttttcttgct ttctgctcct aaaaaagata
     5101 tactttgaca gtgaaataat ttaacactca atgaaatcaa agagcaaact aggaagctag
     5161 ccgcagactg ctcaaagcac tgctttgagg ttgtaaataa gactgacgaa gtcagctcaa
     5221 aatactgttt tgaggttgca gatggaagct gacgcggttt gaagagattt tcgaagagta
     5281 taaactgctt ataaaataaa aaggagccct gatggaacac attatttatc agcttgaaga
     5341 ggacttggca atccttacct tgaaccgtcc tgaggtcgca aatggttttc atattcccat
     5401 gtgtgaggag attttagaag ctctgacttt ggcagaagaa aatccagctg tgcattttat
     5461 cttaatcaat gccaatggaa aggtcttctc agttggggga gatttggtag agatgaagcg
     5521 ggcagtggat gaggatgata ttccatcatt gacaaaaatc gcagagttgg tcaatacgat
     5581 ttcttataaa atcaagcaaa tagccaaacc tgttttaatg gaagttgacg gtgctgttgc
     5641 aggtgccgca gcgaatatgg ctgttgcggc agatttctgt ttggcgacgg ataaggctaa
     5701 gtttatccaa gcctttgttg gtgttggttt ggctccagat gcagggggaa ttcatctctt
     5761 gagccgcagt atcggtgtga cgcgtgctgc tcaattagct atgacaggag aggctttaac
     5821 ggcagaaaaa gccctagaat gggggttggt ttaccgcgtt tctgaagctg aaaaacttga
     5881 aaagacgaga gaacagcttc ttaaaaaatt aagacgtgct tcaagtaatt cctatgctgc
     5941 cattaagaag ttggtttggg agagccaatt taaagactgg cagggttacg ctactttaga
     6001 actgaaccta cagaaatcct tagctcaaac agaggatttc aaagaaggag tgcgagctca
     6061 ttcggagaga agaagaccta aatttacagg aaaataaaaa atacttgcat cattctttga
     6121 attttgatat aattctttct gtcaaatgtt ttgattgtaa aagttttttg aagaaggagg
     6181 gatatgaaaa atggactacc aacgaatcaa tgaatattta acatctatat ttaacaatgt
     6241 ccttgtaatt gaggaagtga acttgagagg tagtcgtttt aaggatatct ccatcaaaga
     6301 aatgcatacg attgatgtca tcggtaaggc tccagatgtg actccaagtc aagtgtcaaa
     6361 agagttaatg gtaactcttg gaactgttac gacaagtttg aacaatttag agcgtaaggg
     6421 ctacattgag cgagttcggt cagaacagga tcgtcgtgtg gtgcatctgc atttgacaaa
     6481 gaagggtcgc ttgattcata gactgcataa acgcttccac aaggccatgg tagaaaaaat
     6541 tattgatggc atgagcgagg aagaaattgc tgtcatgggt aaaggcttga ctaatcttta
     6601 ccaatttttg gaggatttga aataatggct tttgcaaaaa taagtcaggt tgctcattat
     6661 gtgccagagc aagtggttac aaatcacgac ttggctcaga ttatggatac caatgatgag
     6721 tggatttcaa gtcgaacggg aatacgacaa aggcatattt caagaacaga atctaccagt
     6781 gatttggcta cagaggttgc taagaaactg atggcaaaag ctggaataac aggagaagaa
     6841 ctggatttta tcatcctagc taccattact ccagattcga tgatgccctc tacagctgct
     6901 cgtgttcaag ctaatattgg tgctaataaa gcctttgctt ttgacttaac cgcggcttgc
     6961 agtggatttg tatttgctct ttcaactgct gaaaagttta tcgcttctgg tcgctttcaa
     7021 aaaggcttgg tgattggtag tgaaaccctc tctaaggcag tcgattggtc ggatcgatca
     7081 acagctgtgt tgtttggaga tggtgctggt ggtgtcttgt tagaagctag cgagcaagag
     7141 catttcttag ctgagagtct caatagcgat ggaagtcgca gcgagtgttt aacttatggg
     7201 cattcaggtt tgcattctcc attttcagat caagaaagtg cagattcgtt tttgaagatg
     7261 gatggacgca cagtctttga ttttgccatt cgagatgtag ccaagtctat caagcagact
     7321 attgatgaat ctcctataga ggtgacagac ttggattatc tgctacttca tcaagccaat
     7381 gaccgtattt tggataagat ggctagaaaa attggtgttg accgagccaa acttccagcc
     7441 aatatgatgg aatatggcaa taccagtgca gccagtatcc cgattttact ttcagagtgt
     7501 gtagaacaag gtctcatccc tttagatggt agccagactg ttcttctatc aggcttcggt
     7561 ggaggtttga cctggggcac gctcattctt acaatttagg taatcatgtg gtgaacacat
     7621 tgttataaaa aaactattta ttttaaagga gtcctatcat ggcagtattt gaaaaagtac
     7681 aagaaattat cgttgaagaa cttggaaaag acgcatcaga agtaacactt gaatcaactt
     7741 ttgatgattt ggacgcagat tcattggact tgttccaagt aatctcagaa atcgaagatg
     7801 cttttgatat ccaaatcgaa gcagaaaatg acttgaaaac agttggtgac ttggttgctt
     7861 acgttgaaga gcaagcaaaa taagcagaat attagtagaa ggagtaggga gaaacccact
     7921 ccctcttgtt taggtaatag tttgactgtc aaattatggt gaaatcgaac tattacgtaa
     7981 gcaaggaagg ttggaggcac tatgaaaacg cgtattacag aattattgaa gattgactat
     8041 cctattttcc aaggagggat ggcctgggtt gctgatggtg atttggcagg ggctgtttcc
     8101 aaggctggag gattaggaat tatcggtggg ggaaatgccc cgaaagaagt tgtcaaggcc
     8161 aatattgata aaatcaaatc attgactgat aaaccctttg gggtcaacat catgctctta
     8221 tctccctttg tggaagatat cgtggatctc gttattgaag aaggtgttaa agttgtcaca
     8281 acaggagcag gaaatccaag caagtatatg gaacgtttcc atgaagctgg gataatcgtt
     8341 attcctgtcg ttcctagtgt cgctttagct aaacgcatgg aaaaaatcgg tgcagacgct
     8401 gttattgcag aaggaatgga agctgggggg catatcggta aattaacaac catgaccttg
     8461 gtgcgacagg tagccacagc tgtatctatt cctgttattg ctgcaggagg aattgcggat
     8521 ggtgaaggtg ctgcggctgg ctttatgcta ggtgcagagg ctgtacaggt ggggacacgg
     8581 tttgtagttg caaaagagtc gaatgcccat ccaaactaca aggagaaaat tttaaaagca
     8641 agggatattg acactacgat ttcagctcag cactttggtc atgctgttcg tgctattaaa
     8701 aatcagttga ctagagattt tgaactggct gaaaaagatg cctttaagca ggaagatcct
     8761 gatttagaaa tctttgaaca aatgggagca ggtgccctag ccaaagcagt tgttcacggt
     8821 gatgtggagg gtggctctgt catggcaggt caaatcgcag ggcttgtttc taaagaagaa
     8881 acagctgaag aaatcctaaa agatttgtat tacggagccg ctaagaaaat tcaagaagaa
     8941 gcctctcgct ggacaggagt tgtaagaaat gactaaaaca gcctttttat ttgctggtca
     9001 aggtgcccag tatctaggga tgggacggga tttctatgat cagtatccga ttgttaaaga
     9061 aacgattgat cgagcgagtc aggtgctagg ttatgatttg cgttatctca tcgatacgga
     9121 agaagacaaa ctcaatcaga cccgctatac gcaaccagcc attctagcga cttcggttgc
     9181 tatctaccgt ttattgcaag aaaagggcta tcagcctgat atggttgctg gtttgtctct
     9241 tggagaatac tctgccttgg tggcaagcgg cgccttggat tttgaagatg cggttgcctt
     9301 ggtagctaag cgtggagcct atatggaaga agcggctcct gctgactctg gcaagatggt
     9361 agcagttctc aatacgccag tagaggtcat tgaagaagcc tgtcaaaaag cttctgaact
     9421 tggagtggtt actccagcca actataacac acctgcacaa atcgtcattg ctggagaagt
     9481 ggttgcagtt gatcgagcgg ttgaactttt gcaagaagca ggtgccaaac gcttgattcc
     9541 tcttaaggtg tcaggtccct ttcacacctc tctccttgaa cctgctagcc agaaactagc
     9601 tgaaactctg gctcaggtaa gtttttcaga ttttacttgt cccctagtcg gcaatacaga
     9661 agctgctgtg atgcaaaaag aggacattgc tcagctcttg acgcgtcagg tcaaggaacc
     9721 cgttcgtttc tatgaaagta ttggggtcat gcaagaagca ggcataagca actttatcga
     9781 gattggaccg gggaaagtct tgtcaggttt tgttaaaaaa attgatcaaa ctactcactt
     9841 agctcatgtg gaagatcaag cgagtttagt agcactttta gaaaaataga ctaaaataag
     9901 tagaagtttt gaaaggaaaa aaatgaaact agaacataaa aatatcttta ttacaggttc
     9961 gagtcgtgga attggtcttg ccatcgccca caagtttgct caagcaggag ccaacattgt
    10021 cttaaacagt cgtggggcaa tctcagaaga attgctcgct gagttttcaa actatggtat
    10081 caaggtggtt cccatttcag gagatgtatc agattttgca gacgctaagc gtatgattga
    10141 tcaagctatt gcagaactgg gttcagtaga tgttttggtc aacaatgcag ggattaccca
    10201 agatactctt atgctcaaga tgacagaagc agattttgaa aaagtgctca aggtcaatct
    10261 gactggtgcc tttaatatga cacaatcagt cttgaaaccg atgatgaaag ccagagaagg
    10321 tgctatcatt aatatgtcta gtgttgttgg tttgatgggg aatattggtc aagctaacta
    10381 tgctgcttct aaggctggct tgattggctt taccaagtct gtggcacgcg aggtcgctag
    10441 tcggaatata cgagtcaatg tgattgctcc aggaatgatt gagtctgata tgacagctat
    10501 cttatcagat aagattaagg aagctacact agctcagatt ccgatgaaag aatttgggca
    10561 ggcagagcag gttgcagatt tgacagtatt tttagcaggc caagattatc taactggtca
    10621 agtgattgcc attgatggtg gcttaagtat gtagcgaaag ctagaggtga aaagaatgaa
    10681 actaaatcga gtagtggtaa caggttatgg agtaacatct ccaatcggaa atacaccaga
    10741 agaattttgg aatagtttag caactgggaa aatcggcatt ggtggcatta caaaatttga
    10801 tcatagtgac tttgatgtgc ataatgcggc agaaatccaa gattttccgt tcgataaata
    10861 ctttgtaaaa aaagatacca accgttttga taactattct ttatatgcct tgtatgcagc
    10921 ccaagaggct gtaaaccatg ccaatcttga tgtagaggct cttaataggg atcgttttgg
    10981 tgttatcgtt gcatctggta ttggtggaat caaggaaatt gaagatcagg tacttcgcct
    11041 tcatgaaaaa ggacccaaac gtgtcaaacc aatgactctt ccaaaagctt taccaaatat
    11101 ggcttctggg aatgtagcca tgcgttttgg tgcaaacggt gtttgtaaat ctatcaatac
    11161 tgcctgctct tcatcaaatg atgcgattgg ggatgccttc cgctccatta agtttggttt
    11221 ccaagatgtg atgttggtgg gaggaacaga agcttctatc acaccttttg ccatcgctgg
    11281 tttccaagcc ttaacagctc tctctactac agaggatcca actcgtgctt cgatcccatt
    11341 tgataaggat cgcaatgggt ttgttatggg tgaaggttca gggatgttgg ttctagaaag
    11401 tcttgaacac gctgaaaaac gtggagctac tatcctggct gaagtggttg gttacggaaa
    11461 tacttgtgat gcctaccaca tgacttctcc acatccagaa ggtcagggag ctatcaaggc
    11521 catcaaacta gccttggaag aagctgagat ttctccagag caagtagcct atgttaatgc
    11581 tcacggaacg tcaactcctg ccaatgaaaa aggagaaagt ggtgctatcg tagctgttct
    11641 tggtaaggaa gtacctgtat catcaaccaa gtcttttaca ggacatttgc tgggggctgc
    11701 gggtgcagta gaagctatcg tcaccatcga agctatgcgt cataactttg taccaatgac
    11761 agctgggaca agtgaagtat cagattatat cgaagctaat gtcgtttatg gacaaggttt
    11821 ggagaaagaa attccatacg ctatttcaaa tacttttggt tttggaggcc acaatgcagt
    11881 tcttgctttc aaacgttggg agaatagata agtatgaatt taaacgatat taaagacttg
    11941 atgactcaat ttgaccagtc aagtttgaga gaattttctt ataaaaatgg gacggatgag
    12001 ttgcagttta gcaagaatga agcaagacct gtgcctgaag ttgcaactca agtcgctcca
    12061 gcacccgttc tagcaacacc gagtccagta gctcctacat ctgctccagc agagactgta
    12121 gcagaagaag ttccagctcc agctgaagca agtgtggcta ctgagggaaa tcttgtagag
    12181 agtccacttg ttggagtggt ttacttggct gctggaccag ataaacctgc cttcgttaca
    12241 gttggtgata gtgtcaaaaa aggtcaaaca ttggtaatta tcgaagccat gaaagtcatg
    12301 aatgaaatcc cagctcctaa ggatggtgtg gtaacggaaa ttctcgtctc taacgaagaa
    12361 atggttgagt ttggtaaagg attggtacgt atcaaatgat cgatattcaa ggaatcaaag
    12421 aagctcttcc ccaccgttat cctatgcttc tagtggaccg tgtcttggaa gtgagcgagg
    12481 ataccattgt tgctatcaaa aatgtgacca ttaacgaacc tttctttaac ggccactttc
    12541 ctcaataccc agttatgcca ggtgttctga ttatggaagc cttggcgcaa actgctggtg
    12601 tgttggagtt atcaaaacct gaaaataaag ggaaactggt cttttacgct ggtatggaca
    12661 aggttaagtt caagaagcaa gttgtaccag gcgaccaatt ggttatgaca gcgacttttg
    12721 taaaacgtcg tggcaccata gctgtggttg aagcaaaggc tgaagtggat ggcaagcttg
    12781 cagccagtgg tatccttact tttgcaattg ggaactaagg aggttctcca tgtttcgaaa
    12841 aattttaatt gccaatcgtg gtgaaattgc ggttcgtatt atccgtgcgg cacgtgaatt
    12901 ggggattgcg acggtagcgg tttattcaac tgctgataag gaagctcttc atacgctttt
    12961 ggcagatgaa gcagtttgta ttggtcctgg caaggcaaca gagtcttatc tcaatattaa
    13021 tgcagttcta tcagctgcag tcttgactga ggcagaagct attcaccctg gttttggatt
    13081 tctcagtgaa aattccaaat ttgcgaccat gtgtgaagaa gtaggtatca agtttatcgg
    13141 tccatctggt catgttatgg atatgatggg ggataagatc aatgcgcgtg ctcagatgat
    13201 taaagcaggt gtgcctgtta taccaggttc agatggagaa gtgcataact ctgaagaagc
    13261 tttgattgtt gctgaaaaaa ttggctatcc tgttatgctc aaggcttcag caggtggagg
    13321 tggtaaaggg attcgtaagg ttgaaaaacc agatgacctc gtttctgcct ttgaaactgc
    13381 ctctagtgag gccaaggcca attatggcaa tggtgccatg tacatagaac gggttatcta
    13441 tccagctcgg cacattgagg ttcaaatcct aggtgatgag catggacatg tgattcactt
    13501 gggtgaacgg gattgttctc ttcaaaggaa taaccaaaag gttttggaag aaagtccctc
    13561 gattgcaatc ggaaaaacgc tgcgtcatga aataggtgct gctgctgttc gagcggcaga
    13621 gtttgttggc tatgagaatg caggaaccat tgaatttctt cttgatgaag caagtagaaa
    13681 tttctatttc atggagatga atactcgtgt tcaggtagaa catccagtaa cagagtttgt
    13741 ttcaggtgtt gatatcgtta aggaacagat ttgcattgcg gcaggtcagc ctttgtctgt
    13801 taagcaagaa gatattgtcc tacgcggtca tgccatcgag tgtcgtatca atgcagaaaa
    13861 cccagccttt aactttgctc caagtccagg taagattact aatctctatc tgccaagtgg
    13921 tggagttggc ttgcgcgtgg attcagcagt ttatccaggt tataccattc cgccttatta
    13981 tgatagtatg attgccaaaa tcatagtaca cggcgaaaat cgttttgacg ccttgatgaa
    14041 aatgcaacgt gccctctatg aattagagat tgaaggagtg cagaccaatg cagatttcca
    14101 gcttgatctc atttcagatc gcaatgtcat tgctggggat tatgatactt ccttcttgat
    14161 ggaaaccttc ttacctaaat atcaagaaaa agaataaaat gactttaaga gtttaaaccg
    14221 aaaaggggaa tcaatggctc tatttagtaa aaaagataag tatattcgaa tcaatcccaa
    14281 tcgttcggtt agggaaaaac ctcaagctaa gccagaggtt ccagatgaat tattttccca
    14341 gtgtccaggc tgtaagcata ccatctatca gaaggatctg ggaagtgaac gtatctgtcc
    14401 gcactgtagc tatacctttc gtatttctgc ccaagaacgc ttggctttga cgattgatat
    14461 gggaaccttc aaagaattgt ttacagggat tgaaagcaag gatcccttgc atttccctgg
    14521 ttaccaaaag aaactggcat ctatgcgtga aaaaacaggt ctgcatgaag ccgttgtgac
    14581 aggaactgct cttattaaag gtcagactgt ggctcttggg attatggatt ctaattttat
    14641 catggcttct atgggtacgg ttgtaggtga aaaaatcact cgtttgtttg agtatgcgac
    14701 tgtcgaaaaa ttgccagttg tcctattcac agcctctggt ggagcccgta tgcaggaagg
    14761 aatcatgagt ctcatgcaga tggctaagat ctctgcagcg gttaaacgcc attcaaatgc
    14821 tggtctcttt tacctgacca ttttgacaga tccaacgact ggtggtgtga cagcttcttt
    14881 cgctatggaa ggcgatatca ttctggctga accacagagc ttggttggtt ttgctgggcg
    14941 tcgtgtgatt gaaaatacgg ttcgtgaaag cttgcctgag gatttccaaa aggcagaatt
    15001 cctattagaa catggctttg tggatgctat tgtcaaaaga agagacttac cagatacgat
    15061 tgctagccta gtcagattgc atggagggag tcctagatga atattgcaaa aatagtcaga
    15121 gaagcgcgtg agcagagtcg cttgacaacc ttggactttg cgacaggcat ttttgatgaa
    15181 tttatccaat tacatggtga ccgttctttt cgtgatgatg gtgcagttgt tggtggtatt
    15241 ggttggcttg gagaccaagc tgtaacagtg gttggtatcc aaaaaggcaa gagtttgcaa
    15301 gacaacctca aacggaattt tggccaacca catccagaag gctaccgaaa ggcactgcgg
    15361 ttgatgaaac aggctgagaa atttggccgt ccagttgtga cctttatcaa tacagcaggt
    15421 gcttatcctg gtgtcggagc ggaagaacgt ggtcaagggg aagctatcgc tcgcaatctc
    15481 atggaaatga gtgacctgaa agttcctatt atcgccatta ttatcggtga aggtggttca
    15541 ggcggggctc tggctctagc tgtcgcggac cgtgtctgga tgctggaaaa ttctatctat
    15601 gccattctca gtccagaagg ctttgcttcc attttatgga aggacggtac tcgcgccatg
    15661 gaagcagcag aactgatgaa aatcacttcg catgaactgt tagaaatgga cgtggtggat
    15721 aaggtgattt ctgaagtagg actttctagt aaagaactaa ttaagagtgt caaaaaagaa
    15781 ctccaaacgg agctggctag actttcacaa aaaccgctag aagagttgct ggaagaacgc
    15841 tatcaacgat ttagaaaata ctaagaatga ctaaatgaga ctagaaaggt ctcatttttt
    15901 atggaaaaat gcaattcagg tcgaaaaatt gccagttgag gagatagact ataaaataga
    15961 caaaaaaaga aatatactat aaatgtgagc ctacaacaat ctattttaag aaggtacttg
    16021 caatgacagg tacagaaaca tttacagtta tttcaactga ggacttggag caaacttcag
    16081 gtggtcttgc tgtttgggaa gatggatata gtagatggtt atattataga gaatttgctc
    16141 cctatatgag gcaaggggca cttaattctt atatagatgc ttggaagtac ggcttccgaa
    16201 cagggtaatc tttcaaaaaa gtacgatgag aaacacaaag aaactaagac aatttggaat
    16261 atttttgctt attattttac tatctaccta tttaccacaa acaattggat tgtatgtgac
    16321 gatcatttta gggttaggag ctgatgtcta ttctcttatt ttaacagtgg gattagtggg
    16381 aagtttcctt ttctattaat ttggaggtta aaaaagaaaa agagctggaa ctggtcattt
    16441 gtcttctcct tatttgcaac ttatgtggtt tatcagattc tcggtaattt ttgggcacgc
    16501 tatgctcatt tgattaatca taggaatatt catgatgagt attttaccgt ggttctttca
    16561 aatggacaac caaccttttt atcgactatc ttatcttttg tcctgcctgt aatcatcggt
    16621 cctgtttttg aggagactct tgatagaggg tattttatga ataccttctt tccctagtcc
    16681 aagtattatt tagatgtcat cttatcaggc cttatctttg ggcttagtca tttgatacta
    16741 tctcacagag atccaattag tttattatat tatagtctga ttggtttctt ttttgccctt
    16801 gtttatcgtt ctaccgacaa tctgagatta acgattctct gccatagttt ttttaacttt
    16861 ctcaatcatg caaaacctat ctggattttt gtttacaatt atatctatta tcattttttt
    16921 agatagtggg aggcaaagaa aaagtgtttg atagatatca aacacttttt ctttttattg
    16981 ttcttctgtt acaaactggc tgagcagtcc attgataaaa cgggcagatt tttgatcgga
    17041 gaagtccttt gcaagctcga tagcttcatt aacagcaacc agctgaggag tgtcaaatga
    17101 agtgatttca aagactccca agcgaaggag gtttctctcc acgagcgtta agcgttcaat
    17161 ggtccaacct gcttttaaat gctgagtgat ttgcttatct agttcttcct ttttagcttg
    17221 aacaccagaa acgaggtcta tcaaaaaggc tggaagttgt acagccgtat cttcacgatc
    17281 atgagtatag gcgaaacgac aagcagtttc gacatccgta ccgaactcaa ggctcatgag
    17341 agcttgaaaa gcgcatttac ggagttggcg tctagattct aatagtggac tagtcattga
    17401 ggaagtcctc gtcaaataga tctttcaatt ctggttttgg tgttttatct gggacgatac
    17461 ctgcaacgtg aatattgata gcagcgagtt ctacatcagc catattacgg acggcatctt
    17521 tgacagcttt ctggatagca accgctacct taggaacttt tactccgtac tcaaggtaga
    17581 gatagatatc tgctgtgagt tcttcgtcca cgtttttaag ataaatgcca cggccgagtg
    17641 aaagttttga aagggtatca gacactgatc tgtttgaaaa agagtgaaca ccctctacct
    17701 ttgcagtagc gatagcaatg attttttcaa gtacacgtgg ggcgataacg atttcgccaa
    17761 gttgttcttc aattcccata gaatgacctc tttctagaga ttaggcacga gaaacgtaag
    17821 ttccttctgc agtgttgata acgagttttt gtcctgcttc gatgaagtct ggaacgttta
    17881 cgacaagtcc agtttccatc gttgctggtt taccagaacc tgtaacagta gcacctttga
    17941 tagatggttg agtttcagca actgtcaact caacagtagt aggaacggtg acaccgatca
    18001 cttcagttcc gtagaattgg attttcacat cagagttttc aaggatgtaa agcaattcgt
    18061 tttcaacatt gactacaggg atttcgtatt ggtcataagt ttctgtattc atgaagtatg
    18121 ctgtgtcatc cattttgtac aagtattgag ctgggacagt ctcgataata gcttgttcaa
    18181 atttttcctc tggacggtag cttgtgtcaa atgtagaacc agtacggaca tcacgcaatt
    18241 tcatacgcat gatcgtgttt cctttacctg gtttgtggtg actagcttcc aaaacgcgaa
    18301 tcaatttgcc gtcagctgtt tcaaaggtca taccagcttt taatttactt gcttcaatca
    18361 tgtttttacc tctttaataa atattattac tcttcatttt accataaaat actctggaaa
    18421 taaagccaaa atagttattg ggagttggta gagaagaaaa aaccaacctt agggctggtt
    18481 ttgttctgtc tagttttctt tcaacttcgc caattcctgt gcaagtagtt taagggcaac
    18541 ttgtgggttg gcttggcctt tggttgcctt cataaggaat cctgtaaagg ctttgtcggc
    18601 gttacgtttg cctgacttga agtcggcaac agcagcttcg ttatcggcaa agacttggtg
    18661 gatgattggg atcaagatag ctggatctga aatttgaacc atacctgctt tttccacgta
    18721 ttcacgcgcg ccaccgccat ttttagctag atggacaaag actttcttgg caatcttaga
    18781 tgaaatagta ccgtcttcga tgatggcaat catttcaacc aagttttctg gtgtcaattc
    18841 gatttgttcc agtgttttac cttcagcatt caagaactga gcgacttccc cttggagcca
    18901 gttagagact tgtttggcat caccacctag ggcaacagct ttttcaaaga agtcagaagt
    18961 gactttatta gcagtcaact gactagcatc gtagtctgat aaaccaaggt cagatacata
    19021 acgcgcacga cgttcttttg gaaactctgg caactcagtc cgcatttcct caatccactc
    19081 gtcagaaatt tcaaagaggg gtaggtctgg ttctgggaag tagcggtagt cagcagcccc
    19141 ttccttgaca cgcatgagga tggttgcttt attcgcttca tcgtaacggc gtgtttcttg
    19201 gcggatttga ccacctgagc gaagaatttc agcctggcgt tggacttcgt attcaagacc
    19261 tttacgaacg tttgagaagg agttgaggtt cttcaattca gtcttggtac cgaatttctc
    19321 ttgaccataa ggacgaaggg agatgttggc atccacacgc atcgaacctt cctccatctt
    19381 aacgtcagaa atgccagcgt actggataac ttccttgagg gctgtcagat aagcataggc
    19441 ttcttcagga gaacgcatat ctgcctcaga tacaatctca atcaagggaa ccccttggcg
    19501 gttgaggtca acataagagt agccatctgt accatgggtg tttttaccag cgtcttcctc
    19561 taggtgggca cgttcgatac cgattttctt ggtcgtaccg tcttctagtt tgacttcaat
    19621 ccagccatta tatccgattg gttcatcaaa ctgagaaatt tggtaggctt tggggttatc
    19681 aggatagaag tagttcttgc ggtcaaagtg catctttttg tggatgtcca tgttgagggc
    19741 aagagcagcc ttgataccgg catcaacaac ccctttattg agaactggta gaactcctgg
    19801 gaaagaccag tcaatcacgt tagtgttggc attttggtca tttccaaagt gggcagaagt
    19861 aggtgagaag atttttgaat tggtgttgag ctctacgtgg acttcaagtc cgatgactgt
    19921 ttcaaagttc attagttgtc acctccaaaa atcacgggtt gttgtttgtg gtagtctgtt
    19981 gttgcttcaa aagcagcagc agcttggtaa atggtttcct cagagtactt gggaccaatc
    20041 aattggagtc cgacaggtag accttgagag aatccagcag gaatcgaaat tccaggcagt
    20101 cctgccaagt ttacaggtat ggtcaatagg tcggctaagt acatggcaac tgggtcatgg
    20161 ttgagagaat ccaagtcata ggcaacactt ggagcagttg gacccaaaat caaatcgtaa
    20221 tccgcgaaga ctttttcgaa atcttgaatg atgagggtac ggacttgacc agcctttttg
    20281 tagtaggcat catagtaacc tgatgaaaga ctgaaagtac ccagcatgat acgacgtttt
    20341 acctcttcac caaaaccttg gcttcggctg tttacataga tttcatcaag gttggttgca
    20401 tcttctgcgc gatagccgta acggataccg tcgaagcgtt gcaagtttga tgaagcttct
    20461 gatgaagcga tgatgtaata aacggcaaca ccgtatttag agtgaggaag gctgacttct
    20521 tcgacgatag cacccaattt ttcaaagtgt ttggccgcgt ttaagattgt ttccttaacc
    20581 tctggatcaa ttccttcgcc taggtattcc ttaggcaaag cgattttcat acccttgatg
    20641 tcttggccga tttttgaagt aaagtcggcg atgcggacag gagcagaagt agagtcttta
    20701 gcatcttcgc tggcaatagc gttgagcaag agggcatttt ccttaacagt aggagcaaaa
    20761 ggtccaatct ggtctaatga gctaccaaag gcaatgagac cgaaacgtga aactgttccg
    20821 taggttggtt tgagaccaac gattccgttg aaggcagcag gttggcggat ggaaccacca
    20881 gtatcagaac caagtgacaa gcgaacttgt cctgaggcta cagctgcggc agaaccactt
    20941 gatgacccac caggaacctt gctgtggttc caagcgtttt tagttgctcc gtagtgtgaa
    21001 gtttcacctg aaccacccat agcaaattcg tccatgttgg tctttccaac gacaatcatg
    21061 cccttggttt ttgcattggc aacagctgtc gcatcaaaga ttggctcata gttgtagagc
    21121 atttttgagg cagcagttgt gagaataccg tctgtagaga tgttatcctt aacagcaagt
    21181 ggaattcctg aaaggacatt gtcagcatca attccagctt catcaatggc tttagcttga
    21241 acaagagctt gctcctcagc gatggtgaca aatgaattga gggcttcctc acgagacttg
    21301 atattttcaa gtgttgcttg ggtcaattct gttgcagaaa tttccttaga gacaaggaga
    21361 ttgtgcaact cttcaatagt tttattgtta aaagtcatca ggcatctcct ccatcgtcta
    21421 ggatagctgg caccttgata tagtagttgt cttgttcagg tacgttttta aacaagcgat
    21481 cacggtctgt tccttcttcg gccacatcag ggcggagtac agtcttgcgg tcagccatag
    21541 tcgtagtagg tgcgacacca gttgtgtcaa cttcgcccag caattcaacc atgtcaacaa
    21601 tcttagacaa ggtggtcgca aaggcagcag tttcttcttc agagaatctt aattttgaaa
    21661 gattggcaac gtgtgttacc tcttcttgcg taattttcat cttgcatcct ttcgtgaaat
    21721 gatgattttt agtctgttct attttaccat attttcctat aaataaggga gggggaagtg
    21781 aaaagagata gaaattgaaa aaaatgctaa aatccgatat aatggagtat tgaaaggaat
    21841 ggtaaaatcc aatgtaaaaa tcattctcag ctattgaaac aagaaaaata gagaatcaaa
    21901 gaaagagaac ttatgaatat tcaagaagaa attaagaaac gtcgtacctt tgccattatc
    21961 tcccacccgg acgcggggaa aacaaccatc actgagcagt tactctactt tgggggtgag
    22021 attcgtgagg ctggtacggt aaaagggaag aaaacaggga cttttgctaa atctgactgg
    22081 atggatatcg agaagcaacg tgggatttct gttacttcat ctgttatgca atttgactac
    22141 gacggcaagc gcgtgaatat cttagacacg ccagggcacg aggacttctc agaagatacc
    22201 tatcgtacct tgatggcggt ggatgctgcg gtcatggtcg tggactctgc caaggggatc
    22261 gaggctcaaa ccaagaaatt gtttgaggtt gtgaaacatc gtggcattcc agtttttacc
    22321 tttatgaaca agctggaccg tgacggtcgt gagcctttgg atctcttgca agaattggaa
    22381 gaaatcttgg gcattgctag ctaccctatg aactggccta tcgggatggg gaaagccttt
    22441 gagggcttgt atgacctcta taaccaacgt ttagagcttt acaaagggga tgagcgtttt
    22501 gccagtctag aagacgggga caaactcttt ggtagcaatc ctttctacga gcaagtcaag
    22561 gatgacattg agcttttaaa tgaagctggg aatgagtttt cagaggaagc tattctggct
    22621 ggagaattga cgcctgtctt tttcggttca gccctgacaa actttggtgt gcagaccttc
    22681 cttgaaatct tcctcaagtt tgctccagaa ccacatggtc acaagaaaac agacggtgaa
    22741 attgtggatc cttatgacaa ggatttctca ggctttgtct ttaaaatcca agccaacatg
    22801 gatcctcgtc accgtgaccg tattgccttt gtccgtatcg tatcaggcga atttgagcgc
    22861 ggcatgagtg tcaatctccc tcgtactggt aagggggcta aactatctaa tgttacccag
    22921 tttatggcgg aaagtcgtga gaatgtgatc aatgccgtgg caggtgatat tatcggggtt
    22981 tacgataccg gtacttatca ggttggggat accttgacgg ttggaaaaaa caagtttgaa
    23041 tttgaaccac tgccaacctt tactcctgaa attttcatga aagtttctgc caagaatgtt
    23101 atgaagcaaa aatccttcca caaggggatt gagcaattgg tgcaagaagg agccgttcag
    23161 ctttataaga attaccaaac aggtgagtac atgctgggag ctgttggtca actccagttt
    23221 gaagtcttta aacaccgtat ggaaggcgaa tacaatgcgg aagtggtcat gagcccaatg
    23281 ggtaaaaaga ccgttcgttg gatcaagcct gaggacttgg atgaacggat gtcgtcaagt
    23341 cgcaatatct tagccaaaga ccgttttgac caaccagtct ttctctttga aaatgacttt
    23401 gccctccgct ggtttgcgga caagtatcca gacgtagagt tagaggagaa gatgtgactc
    23461 agtaccaaca attggagcta aagttccaaa ttgttggacg ctagtcgcta tttggcgaac
    23521 tagctaactg cctcactaac tacgtttggc aaatgaaatc gatttgtaaa actccgtgtc
    23581 gtaatgtaaa aattgtaaac cgtaatggga cttcatctaa aatctccact ggtatggttt
    23641 tacgaacgaa taggcgaaaa taatggttac gtagaaacat atgatgggaa taatatgatg
    23701 ggaataaaaa gaaataagat aaagagtgaa agaagagaaa aaagcgattg tcttaggtgc
    23761 agataatgct tatatggata aagttgaaac aacaataaaa tctctttgtg ttcatcatta
    23821 taatcttaag ttttatgttt ttaatgatga ccttccgcga gaatggttcc aattgatgga
    23881 aaaaagacta gagactttga atagtgagat tgtgaatgtt cagattgaca gtagtattct
    23941 gaaaggttat cgtctcccct ttgagggact ttcatatgcg gcttttttta ggtattttat
    24001 tccaaagtat gtaagcgagt ctcgtgtact gtatttggat tcagatattg ttgtaagaaa
    24061 atcgatagat gagctgtggg atttggactt gacggctata cccttagctg cagttagaga
    24121 tgatttttat acacataatt ttaattctgg tgttctgtta atcaacaatg gcatgtggag
    24181 agcagaaaat gtcacgcaag acctgattta agacacaaat aaaaaatctt ccaattccct
    24241 tgcaaaaaac aagaaagtgt gataacatag tacggtatgt ggtgctagca catccgctat
    24301 attagatcta ataggaggaa aacacaatgg ctaaagtatg ttactttaca ggtcgtaaga
    24361 ctgtatcagg aaacaaccgt tcacacgcga tgaaccaaac aaaacgtgcc gtaaaaccaa
    24421 accttcaaaa agttactgtt cttatcgatg gtaaacctaa aaaagtttgg gcttcagctc
    24481 gtgctttgaa atcaggtaaa gttgaacgcg tttaataata aaagcaaagg agaccttagg
    24541 gtcttttttc ttttattgtg ggtataacat cgtttgaaaa atagagtaaa tatccgctga
    24601 tacatcattc tgcttttaca cttgggctga aatatgataa aatagagtat caactagttg
    24661 aggtaaaaag gatgactgta aaaattaata caaaagatgg tcaaatcgaa ctaacagatg
    24721 aagtgattgc aaccgtcgta ggtggagcag caactgagat ttttggtgtg gtcggtatgg
    24781 ctagtaaaaa tgccctcaaa gataatttcc aagccctgct aggtaaggaa aattattcta
    24841 aaggtgtcgt cgtaaaggca gccgaagatg gcagtattgc agttgatgta tataccgtgt
    24901 taagctacgg aacaaagatt agtgaagtgt caaaaaacat tcaagagcgt gttgccttta
    24961 gcttggaaaa ccagcttgga attactgctc agactgtaaa tgtctacatt caaaatatca
    25021 aagttgtagg agaataaccg tgtcaaaaat tactactagc ttatttcaag aaatggtgca
    25081 ggctgcatca actcgcttga ataagcaagc tgaatatgtc aattcattaa acgtctttcc
    25141 agttccagat ggagatactg ggacaaatat gggaatgacc attgaaaatg gtgctaaaga
    25201 agttgcagac aagccagctt ctacagttgg agaagtagcg agcattcttg ccaaagggct
    25261 tttgatgggt gcgcgtggga actcaggagt gattacgtct cagcttttcc gtggattttc
    25321 ccaagctatc aaggataaag acgagttaac aggtcaagac ttggctctgg ccttccaatc
    25381 aggtgtggaa gttgcctata aggcagtgat gaaacctgtt gaaggaacga ttttgacagt
    25441 ttctcgtgga gctgctatcg gtgctaagaa aaaagctgag caaacagatg acactgttga
    25501 agtcatgcgc gcagccttgg aaggtgctaa aacagctcta gctaaaacac cagacatgct
    25561 tccagtattg aaagaagttg gcgttgtgga ctcaggtggt caaggattgg tcttcatcta
    25621 cgaaggtttc ctttcagccc ttactggcga atatattgca tctgaggact ttgtagcgac
    25681 tcctgccaac atgagtgaga tgatcaatgt agagcatcat aagtctgtag ctggtcacgt
    25741 agcgactgag gacatcacgt ttggttactg tactgaaatc atggtagctc ttaagcaagg
    25801 tccaacctat gctaaagatt ttgactacga tgaattccgt aactacttgg atgagcttgg
    25861 agattctctc ctcgttgtta acgatgatga aattgtcaaa gtccatgttc atacagaaga
    25921 tccaggactt gttatgcaag aaggtctcaa atatggtagc ttggtcaagg taaaagttga
    25981 caatatgcgt aaccaacacg aagcacaggt tgagaaagaa gctactcaag ttatcaagtc
    26041 ggctgaagaa aaagagtatg ctttgattgc tgtggtggct ggtaaaggtc tagcagatat
    26101 cttctgttct caaggcgtgg attatgttat cgaaggcggt caaaccatga acccttcaac
    26161 agaagacttt atcaaggctg ttgaacaggt taatgcccgt aacatcatct tcttgccaaa
    26221 caacaagaac atcttcatgg cagctcaatc tgcggcagaa gttttggagc aaccagcggt
    26281 agtggtagag gctcgcactc ttcctcaagg tatgacaagt cttcttgcct ttgatccaag
    26341 caagtccatt gaagaaaacc aagagcgtat gacagctgct cttagcgatg tcgttagcgg
    26401 aagcgtcaca acagccgtgc gtgatacaac gatcgatggc ttagaaatcc atgaaaacga
    26461 taatttaggt atggtggatg gaaaaattct tgtgtcaaac cctgatatgc accaaacatt
    26521 gactgaaacc ttgaaacata tgttggatga agacagtgaa atcgtaacct tctatgtcgg
    26581 tgaagacgga agcgaagaac ttgccaatga aatcgctcaa gaaatcgtag aagaattcga
    26641 agacgttgaa gtcgagattc accaaggtca acaacctgtt tacccatacc tatttagtgt
    26701 ggaataagat taatgaagga ctgagaaatc agttcttttt tctttttagt aaatgaaatc
    26761 ggtatctttt taataaaaac aaaaataaca ttcataaata aacgttaaaa tagaaaattc
    26821 a
//
LOCUS       DAVLFB010000019        25872 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLFB010000019 DAVLFB010000000
VERSION     DAVLFB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.019"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            32..2011
                     /locus_tag="VXD58_000714"
     CDS             32..2011
                     /locus_tag="VXD58_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675456.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKTSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKANAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2078..3574
                     /locus_tag="VXD58_000715"
     CDS             2078..3574
                     /locus_tag="VXD58_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675457.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3574..4500
                     /locus_tag="VXD58_000716"
     CDS             3574..4500
                     /locus_tag="VXD58_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675458.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4509..5576
                     /locus_tag="VXD58_000717"
     CDS             4509..5576
                     /locus_tag="VXD58_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675459.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5587..6513
                     /locus_tag="VXD58_000718"
     CDS             5587..6513
                     /locus_tag="VXD58_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675460.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7080..7940
                     /locus_tag="VXD58_000719"
     CDS             7080..7940
                     /locus_tag="VXD58_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0675461.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            7952..8845
                     /locus_tag="VXD58_000720"
     CDS             7952..8845
                     /locus_tag="VXD58_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675462.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            8858..9661
                     /locus_tag="VXD58_000721"
     CDS             8858..9661
                     /locus_tag="VXD58_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0675463.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(9694..10404)
                     /gene="treR"
                     /locus_tag="VXD58_000722"
     CDS             complement(9694..10404)
                     /gene="treR"
                     /locus_tag="VXD58_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV0675464.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            10589..12556
                     /gene="treP"
                     /locus_tag="VXD58_000723"
     CDS             10589..12556
                     /gene="treP"
                     /locus_tag="VXD58_000723"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV0675465.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            12734..14359
                     /gene="treC"
                     /locus_tag="VXD58_000724"
     CDS             12734..14359
                     /gene="treC"
                     /locus_tag="VXD58_000724"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV0675466.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            14615..14863
                     /locus_tag="VXD58_000725"
     CDS             14615..14863
                     /locus_tag="VXD58_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV0675467.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            15070..15864
                     /gene="racE"
                     /locus_tag="VXD58_000726"
     CDS             15070..15864
                     /gene="racE"
                     /locus_tag="VXD58_000726"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV0675468.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            15861..16832
                     /locus_tag="VXD58_000727"
     CDS             15861..16832
                     /locus_tag="VXD58_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV0675469.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            16808..17329
                     /locus_tag="VXD58_000728"
     CDS             16808..17329
                     /locus_tag="VXD58_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV0675470.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17326..17787
                     /locus_tag="VXD58_000729"
     CDS             17326..17787
                     /locus_tag="VXD58_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV0675471.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            17778..18518
                     /gene="xerD"
                     /locus_tag="VXD58_000730"
     CDS             17778..18518
                     /gene="xerD"
                     /locus_tag="VXD58_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV0675472.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            18518..19246
                     /locus_tag="VXD58_000731"
     CDS             18518..19246
                     /locus_tag="VXD58_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV0675473.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19265..19834
                     /locus_tag="VXD58_000732"
     CDS             19265..19834
                     /locus_tag="VXD58_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV0675474.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            19821..20543
                     /locus_tag="VXD58_000733"
     CDS             19821..20543
                     /locus_tag="VXD58_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV0675475.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            20543..20785
                     /gene="yidD"
                     /locus_tag="VXD58_000734"
     CDS             20543..20785
                     /gene="yidD"
                     /locus_tag="VXD58_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV0675476.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(21010..21975)
                     /locus_tag="VXD58_000735"
     CDS             complement(21010..21975)
                     /locus_tag="VXD58_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675477.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(22071..22823)
                     /locus_tag="VXD58_000736"
     CDS             complement(22071..22823)
                     /locus_tag="VXD58_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0675478.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(22820..23776)
                     /locus_tag="VXD58_000737"
     CDS             complement(22820..23776)
                     /locus_tag="VXD58_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV0675479.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMVFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(23766..24725)
                     /locus_tag="VXD58_000738"
     CDS             complement(23766..24725)
                     /locus_tag="VXD58_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675480.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSILFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(25148..25849)
                     /gene="galE"
                     /locus_tag="VXD58_000739"
     CDS             complement(25148..25849)
                     /gene="galE"
                     /locus_tag="VXD58_000739"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0675481.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT         7423 a         5001 c         5874 g         7574 t
ORIGIN      
        1 tctataattc acataaacag aggagtagaa aatgaaaaaa aatcgtgtat ttgctacagc
       61 aggtcttgtt ttattagcag caggtgtact tgcagcatgc agttcttcaa aaacatctga
      121 ttcatcagcc cctaaagctt atggctatgt ttatacagca gacccagaaa ccttggacta
      181 cctgatttca agtaaaaata gtacaacagt agtgacttca aatgggattg atggtttatt
      241 cactaacgat aattacggta atcttgctcc tgcagttgca gaggattggg aagtctctaa
      301 ggatggtttg acctacactt ataagattcg taaaggggtt aaatggttta cctctgatgg
      361 agaagaatat gcagaggtga cggctaaaga tttcgtgaac ggtttaaaac acgcagcaga
      421 taaaaaatca gaagctatgt atttagctga aaattcggtt aaaggcttgg cagattatct
      481 atcaggaact tcaacagatt tttcaacagt tggtgtcaag gcggttgatg attatacgtt
      541 acaatacact ttgaaccagc ctgaaccgtt ctggaactct aagttgacct attctatttt
      601 ctggcctctg aatgaagaat tcgaaacatc aaaaggaagc gattttgcta aaccaacaga
      661 tccgacatcc ttgctttata atggtccatt cttgttgaaa gggttgactg caaaatcttc
      721 tgtagagttt gtaaaaaatg agcaatattg ggataaagaa aatgtccacc tagatactat
      781 caatctagct tactatgatg gatcagatca ggagtcgcta gagcgtaact tcactagtgg
      841 agcttatagt tatgcccgtc tttaccctac cagctccaac tattctaagg ttgcagaaga
      901 atacaaggac aatatctatt acacacaatc aggctctggg attgctggtc tgggtgtgaa
      961 tattgatcgc caaagttaca actatacttc taaaactaca gattcagaga aagtagctac
     1021 taagaaggca ttgcttaaca aagatttccg tcaagccttg aattttgctc ttgatcgctc
     1081 agcttactca gctcaaatca atggtaaaga tggagcagct ttagcagttc gtaatttatt
     1141 tgtaaaacca gactttgttt cagctggtga gaagaccttt ggtgatttag tcgctgctca
     1201 acttcctgct tatggtgatg agtggaaagg tgtgaattta gctgatgggc aggatggttt
     1261 attcaatgct gacaaggcca atgcagagtt tgcgaaagct aagaaagctt tagaagcaga
     1321 cggcgttcag tttcctattc atctggacgt tccagtagac caagcatcaa aaaactatat
     1381 atctcgtatt cagtccttta aacaatctgt agaaacagtt cttggtgttg aaaatgtcgt
     1441 tgttgatatt caacaaatga caagtgatga attccttaat attacttact atgctgccaa
     1501 tgcttcatct gaggattggg atgtatcggg aggagtttca tgggggccag actatcaaga
     1561 cccatctact tacctggata ttttaaaaac aactagcagt gaaactacaa aaacatattt
     1621 aggatttgat aatccaaata gcccttcagt agttcaagtt ggtttgaaag aatacgataa
     1681 attagttgat gaagctgcca aagagacaag cgacttgaat gtccgttatg aaaaatatgc
     1741 agcggctcaa gcatggttga cagatagttc actctttatt cctgctatgg cttcttctgg
     1801 tgcagcacca gtgctttcac gaattgttcc atttactgga gcttctgcgc aaacaggctc
     1861 taaggggtca gatgtttact tcaaatattt gaaattacaa gataaagcgg tgactaagga
     1921 agagtatgaa aaagctcgtg aaaaatggtt gaaagaaaaa gctgaatcaa atgagaaagc
     1981 tcaaaaagaa ttggcaagtc atgtgaagta aataatttct aatagaactt tctctccatg
     2041 aggagaaggt tcttttggga tttttaaagg aaataatatg aaaaaatata tttttatgcg
     2101 tgttttgcgg tcattggttt cgattttctt agtaacgact ttgacctaca cgattatcta
     2161 taccttggtt cctcgaaaat tgattttcaa gcaggatcct aactataata aaattgcgac
     2221 aacggctgat aaacgtgata actatgaaaa tactgtgttt gagcgtatgg gctacattga
     2281 gtattacgat actaaagagt tgcaagaaaa ggcaagtagc atggattctt ctgtaacagt
     2341 agaagcaaat gcgaccaata aagctattta tgaaaagtac atcaatcaat taggtcatgg
     2401 ttggactttg ggagaattta ctgaaagtgg tcaattctat gctactcgtg aaattccaat
     2461 ttttgaacgt gtttttcact tctatgctaa cttgattgac attgaccata caaataaaat
     2521 ccaagaccct gaaaatccag acttgaaacg ctaccttcgt tttgaaaacg atccagctat
     2581 cggatggtca ttggtcggtt caggaactaa acataaatat ctcttgtact ttaacagtca
     2641 gttcccattt gttcatcaaa actttgtgaa cttgaattta ggtgactctt acccaaccta
     2701 tgctaataca ccagttcttc aggttattac tcaaggtcaa ggacaaacca aaactgccca
     2761 agttcagttc ccaacaggta agaaaacgtc ttctgtaaat atttactcaa gaacctacaa
     2821 gtcacctagt caggctgact ctcgtgaagt agctagctat gggaaagatg atccttatac
     2881 agcgactgaa agtaattacc aatatccatc tatgattgtc agctctgcta ttactggttt
     2941 gattggtttg gttcttgcct atgctcttgc cgtgccactt ggttcagcca tggctcgttt
     3001 caagaacact tggattgata gcctctcaac aggggctttg accttcttgc ttgctcttcc
     3061 aacgattgcc ttggtttaca tcgttcgatt gattggatca tctatttccc ttccagattc
     3121 attccctatc ttgggagctg gagattggcg ttcttacgtt ttaccagcag tcatccttgg
     3181 tttgttgggt gctcctggta cagccatttg gattcgtcgt tacatgattg acttgcaatc
     3241 tcaagacttt gttcgtttcg ctcgtgcaaa aggtttgtct gaaaaagaaa tttcaaacaa
     3301 acacatcttt aaaaatgcca tggttccgct ggtttcagga attcctggtg ccattattgg
     3361 ggttatcggt ggtgcaaccc ttactgaaac agtcttcgcc ttcccaggta tgggtaaaat
     3421 gttgattgac tctgtaaaag catctaataa ctctatggtc gttggtcttg tcttcatctt
     3481 tacatgtatt tctatcttct cacgtctttt gggagatatt tggatgacta ttattgaccc
     3541 acgtattaaa ttgactgaga aaggaggcaa ataatgtcta caatcgataa agaaaaattt
     3601 cagtttgtaa aacgtgacga ttttgcctct gaaactattg atgcgccagc atattcttac
     3661 tggaaatcag tgtttaaaca atttatgaag aaaaaatcaa ctgtagtcat gttgggaatc
     3721 ttggtagcca tcattttgat aagtttcatc tacccaatgt tttctaagtt tgatttcaat
     3781 gatgtcagca aggtaaacga ctttagtgtt cgttatatca agccaaatgc ggagcattgg
     3841 ttcggtactg acagtaacgg taaatcgctc tttgacggtg tctggttcgg agctcgtaac
     3901 tccatcctca tttctgtgat tgcgacagtg attaacttgg ttatcggtgt ttttgtcggt
     3961 ggtatttggg gtatttcaaa atcagttgac cgtgtcatga tggaagttta caacgtcatc
     4021 tcaaacatcc cacctctttt gattgttatt gtcttgactt actcaatcgg agctggattc
     4081 tggaatctga tttttgccat gagcgtaaca acatggattg gtattgcctt catgatccgt
     4141 gtgcaaatct tgcgctatcg tgacttggaa tacaacttgg cgtcacgtac tttgggaaca
     4201 ccaaccttga agattgttgc caaaaatatc atgcctcaat tggtatctgt tattgtgaca
     4261 accatgactc aaatgcttcc aagctttatc tcatacgaag ccttcttgtc tttcttcggt
     4321 cttggattac cgattacagt gccaagtttg ggtcgtttga tttcggatta ttcacaaaac
     4381 gtaacaacca atgcttactt gttctggatt ccattgacaa cccttgtctt ggtatccttg
     4441 tcccttttcg tagttggtca aaacttagcg gatgctagtg atccacgtac acatagatag
     4501 gagtagaaat gacaaaagaa aaaaatgtaa ttttgactgc tcgcgatatt gtcgtggaat
     4561 ttgacgttcg tgacaaagta ttgacagcca ttcgcggcgt ttcccttgaa ctagtcgaag
     4621 gagaagtatt agccttggta ggtgagtcag gatcaggtaa atctgttttg acaaagacct
     4681 tcacaggtat gctcgaagaa aatggtcgca ttgcccaagg tagtattgac taccgtggtc
     4741 aggacttgac agctttatct tctcacaagg attgggaaca aattcgtggt gctaagattg
     4801 cgactatctt ccaggaccca atgactagtt tggaccccat taaaacaatt ggtagtcaga
     4861 ttacagaagt tattgtaaaa caccaaggaa aaacagctaa agaagcgaaa gaattggcca
     4921 ttgactacat gaataaggtt ggcattccag acgcagatag acgttttaat gaatacccat
     4981 tccaatattc tggaggaatg cgtcaacgta tcgttattgc gattgccctt gcctgccgac
     5041 ctgatgtctt gatctgtgat gagccaacaa ctgccttgga tgtaactatt caagctcaga
     5101 ttattgattt gctaaaatct ttacaaaacg agtatcattt cacaacaatc tttattaccc
     5161 acgaccttgg tgtggtggca agtattgcgg ataaggtagc ggttatgtat gcaggagaaa
     5221 tcgttgagta tggaacggtt gaggaagtct tctatgaccc tcgccatcca tatacatgga
     5281 gtctcttgtc tagcttgcct cagcttgctg atgataaagg ggatctttac tcaatcccag
     5341 gaacacctcc gtcactttat actgacctga aaggggatgc ttttgctttg cgttctgact
     5401 acgcaatgca gattgacttc gaacaaaaag ctcctcaatt ctcagtatca aagacacatt
     5461 gggctaaaac ttggcttctt catgaggatg ctccaaaagt agaaaaacca gctgtgattg
     5521 caaatctcca tgataagatc cgtgaaaaaa tgggatttgc ccatctggct gactaggagg
     5581 aaggaaatgt ctgaaaaatt agtagaaatc aaagatttag aaatttcctt cggtgaagga
     5641 agtaagaagt ttgtcgcggt taaaaatgct aacttcttta tcaacaaggg agaaactttc
     5701 tcgcttgtag gtgagtccgg tagtgggaaa acaactattg gtcgtgctat catcggtcta
     5761 aatgatacaa gtaatggaga tatcattttt gatggtcaaa agattaatgg taagaaatcg
     5821 cgtgaacaag ctgcggaatt gattcgtcga atccagatga ttttccaaga ccctgccgca
     5881 agtttgaatg aacgtgcgac tgttgattat attatttctg aaggtcttta caatcaccgt
     5941 ttatttaagg atgaagaaga acgtaaagag aaagttcaaa atattatccg tgaagtaggt
     6001 cttcttgctg agcacttgac tcgttaccct catgaattct caggcggtca acgtcaacgt
     6061 atcggtattg cccgtgcctt ggtcatgcaa ccagactttg ttattgcaga tgagccaatt
     6121 tcagccttgg acgtttctgt acgtgcccaa gtcttgaact tgctcaaaaa attccaaaaa
     6181 gagctcggcc tgacctatct cttcatcgcc catgacttgt cggttgttcg ctttatttca
     6241 gatcgtatcg cagttattta caagggtgtt attgtagagg ttgcagaaac agaagaattg
     6301 tttaacaatc caattcaccc atatactcaa gccttgcttt cagcggtacc aatcccagat
     6361 ccaatcttgg aacgtaagaa ggtcttgaag gtttacgacc caagtcaaca cgactatgag
     6421 actgataagc catctatggt agaaatccgt ccaggtcact atgtttgggc gaaccaagcc
     6481 gaattggcac gttatcaaaa aggactaaac tagtaatggt tttataattt ccatgtcaat
     6541 ttttatggaa attataaaac tttttttgtt ggactgattt gaaaaacttt gaaagcttcc
     6601 tgaaagaatt tttgaaaaat tttaaaaatt atgaaatgtt attgacaggt gatgaaaaag
     6661 gtggtaggac aggaagcatc aaaaaagaaa gcagaaaaag aaatgaataa gagacaagcc
     6721 gtaactatga atcaaaactt ttactttagc tactttagat agtgtttgta gacatgtcac
     6781 catggatgca aacagttcaa gcaatttgtt tgtatctatg tggctaagat ttttgattgt
     6841 ggtccatata gatgaatatc taaatggact agctaagttg taacttgtta ggttatttca
     6901 agtatccgtt tagatagtat ctaggcggtt ttttgtttta tcttttctga gaaggagttt
     6961 cattatgaaa gttgttcgaa aatcactagc cccctcttgg tagtggggct cctcttgact
     7021 tctctgatca gtttgcatca gttgaaggca gataagaaaa agatgtcttt cgtatcggta
     7081 tttcgcaatt tattacccac cagtccttat acgctactag agaaggattt gtggatgagc
     7141 tggccaaaca aggctatgtt gagggggaaa atattgagat tgatttgcaa aatgcacagg
     7201 gagaacaaag aaatctaaaa acgatttccc agcaactagc agaatccagc gatgtcgttc
     7261 tagccatcgc aaggccttct gctcagagct tggccaatac gacgcaaacg acaccagtta
     7321 tcttttcagc tgtaacagac ccagtcagtg ccaagttggt tgagtcaaga gaacatcctg
     7381 ggggcaatgt aactgggaca agcgatcagt catcagatgc catttcaacc caaatcaatt
     7441 tgataaagaa agtgttgcca aaggctaaaa cgattggaat cctctatact cagagcgagc
     7501 ccaattcggt tgttcaaaag gatgaagcta agcgacttct gaaagaaaaa ggctttaccg
     7561 ttgttgaaaa aacaatcttg gacagtaata acgtcaaggc ggcagcagag agtttgatgg
     7621 cagaggtgga tatggttttt gtcccaactg ataatatcat ttcgttaacc atggaaacag
     7681 ttaagcaggt ttctattaaa cacaaggttc cagtattcgg tggttcaaca gaaatgattg
     7741 ctgttggtgg cttgtataac tacggaacca attatgaaga attggtacgt cagacagctc
     7801 gcatgctgat tcgtgtttta aaaggtgaga aaccagaaaa tatagcagtt gagttgcctg
     7861 aaaaactgga attgcatacc aatcaagaaa tggcagatgc attgggaatt gatattagta
     7921 agttagaagg caaggaataa ggaggattaa gatgaatttt gtgttatcta gtttatcaga
     7981 aggtttgttg tggtcgattg tggcgattgg ggtctacttg actttccgta ttttggatat
     8041 tgcggatatg actgctgagg gagcctttcc tctgggggca gcagtcgttg tatcacagat
     8101 acaggcaggg gcaaatcctt ggcttgcgac tttacttgct ttgctggcag gtatggtagc
     8161 aggtcttgta tcaggaatgc tccacaccaa gatgaaaatc ccagctctct tgacagggat
     8221 tgtgaccttg acagggctct attcaatcaa tattaaaatc atgggcagtg tacccaatct
     8281 ttccttggga gattctgcaa ccgtctttaa acagttggcg agcttgggtc tgacaaatga
     8341 aggaactgtt ttctcactta gtttggcctg ttttttactt gtttgtttgg tcttgaccct
     8401 tttgatgaaa acagagattg gcctggtctt gcgttcgact ggggataata tcccgatgag
     8461 tgaggccaat ggagtcaatg tagacaccat gaagattgtt ggttacatga tttcaaacgg
     8521 tttgattgct ctatgtggtt ccttgtttgc ccaaaatgat ggattttcag atgtgacttc
     8581 tgggacagga accattgttg ttggtttgag ctcggtgatt attgcggaag tcttgataca
     8641 cgacttgacc attggaggcc gcttattatc catcggaatc ggtgctattg tttaccgttt
     8701 gattatttta aatatctatg aaattccaaa tctagatcaa aacttggtac gtctctttaa
     8761 tgcaatcttg ctcgctttgg ttttatttgc accagaattg caaaagagat taaagattcg
     8821 tggtctgaaa ttgagaaatg aataggagga gaaagttatg acaagtttgt taacacttga
     8881 aaatattcac aagacatttg aagcagggac ggtcaatgag aaccatgtcc tcaaaggatt
     8941 agatttagag gttgaagagg gagattttat ctctgtgatt ggtggaaatg gagcaggaaa
     9001 atccactttg atgaatatct tggctggcaa tctatcggtg gacgaagggg accttctact
     9061 ggcagggaaa tccattaaaa atctgagtgt cagaaagaga gccaaggata ttgcccgtgt
     9121 ttttcaagat cctaagatgg gaacagcttc tcgtttgacg attgaggaga atatggctat
     9181 tgccttgaga cgcgggcaga agagagggct tggttggggc gtgaaggaga aggatagaat
     9241 ccagttccaa gaggccttga aggagttgaa tattggtctt gaaaaccgct taaaagtaga
     9301 tactcaatat ctctcaggag gacaaagaca ggctttgact ctagtcatga cagatctgat
     9361 gaaaccaaaa cttttacttt tggatgaaca tactgcggca cttgacccga aaaccagtca
     9421 aatggtgatg gacttgacgc aaaagattgt ggagcaccat cagtggacga ctttgatgat
     9481 tacccatgat atgaatcatg cgattgagta cggcaatcgt ctcattatgc tctatcaagg
     9541 caagatagtg gtggatgtca agggagaaga gaaaaagcat ctgacagttg aagaccttat
     9601 gcatctcttc cagaaaaata gtggccaaag tctggtcagt gatgaattag ttttgggata
     9661 aagaaaaagg ctgagatctg gtgtctcagt cttttatttc ttgcgttttg caaaatctac
     9721 aaatctaaat ttctctaact tatggcgact ttcggtaaac tgaaattggc gtccgtcttg
     9781 gagatagact tttgatttaa tcgaaacgac ataagggtct ttgccaatgt ccatgagaat
     9841 cttgtctcgg tcgcttgagt ggtcaatggt gatttccttc tgagcataat caataaggag
     9901 tttgaggcca ttttctatat aagaatagat agactgctca gcaatttggc gagtgagatt
     9961 tggaattagt tccatatcca gatagtccgt atccagaacg gataccagat catccaccac
    10021 acgctggcgg accaccttcc aaaccatccg aaactctggg aaaccggtta tcagtgagga
    10081 ttttttatca ataataatct tgtccagact gaccacgttg gttttagagc gcagtccaag
    10141 ttctttaact agttcttggt agctggttag gttggataca gggaaattga cggtttcttc
    10201 tttgacgact tgagaacctt gctctcttat ctttttgatc aatccttcct cttggaggag
    10261 agacagggcc tttcggacgg tatcacgact cacttgatat tgctccataa ggtcgtgctc
    10321 gctaggaagg aaatctccga cagcgtaagt ctcgttttga atggtttctt ggatttgctt
    10381 aaataattgt tggtatttct tcattttgcc tgctttaata ggtttttagt ctagtttaat
    10441 gaacacctgt atctatttta ccatacaagt gctagaaaat ttacaaaaaa acaccctttt
    10501 tataccaaac aagttgcaga caagtttggt atcgtttaca atatacttat caaatcaaac
    10561 ttgctttgac aagtataagg agaatcaaat gggaaaattt gaacaagaag ccaaagatct
    10621 gcttcaggca attggaggca aagaaaatgt gactgccgta actcactgtg cgacacggat
    10681 gcgctttgtt ttaggagatg ataagaaggc taatgttaaa gctatcgagt caattccagc
    10741 tgttaaagga acttttacaa atgcaggtca atttcaggta atcattggaa atgacgtgcc
    10801 catcttttat aatgatttta cagccgtttc aggtattgag ggtgtttcca aagaagcagc
    10861 caagtctgca gctaagagta atcaaaacgt ggtccaacgt gttatgacca ctctggcgga
    10921 gatttttact ccgattattc cagccttgat agtcggagga ttgatcctcg gtttccgtaa
    10981 tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag accatcacag aatcctctca
    11041 gttttgggca ggtgtcaatc acttcctctg gttgcctggt gaagctatct tccagttctt
    11101 accagtaggg attacttggt ctgtttctcg taagatggga accagccaaa ttttgggaat
    11161 tgttctcgga atctgtttgg tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac
    11221 gccagcagct gatatcgcgg caaactgggt ttggaatttt ggctatttta ctgttaatcg
    11281 tatcggttac caagcccaag ttatcccagc cttgcttgca ggtttgagtc tgtcttatct
    11341 tgaaatcttc tggcgcaagc atatcccaga agtcatttct atgatttttg tacctttctt
    11401 gtcattgatt ccagccttga ttttggctca tactgttttg ggaccaatcg gttggacaat
    11461 tggacaagga ctttcatcag ttgtcttggc aggtttaact ggtccagtta aagggctctt
    11521 cggtgcaatt tttggtgccc tctacgctcc atttgtcatc acaggtctgc accatatgac
    11581 caatgccatt gatacacaat tgattgcgga tgctggtggc actgccctat ggccaatgat
    11641 tgctctttct aatattgctc aaggctcagc cgtgtttgcc tattatttca tgcatcgcca
    11701 tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt tcagcctatc tcggtgttac
    11761 agaaccagct ctttttgggg ttaacgtaaa atatatttat ccatttgttg ctgggatgac
    11821 tggttcagcc cttgcaggca tgttatccgt tacttttaat gtaactgcgg cttctattgg
    11881 tatcggtggt ttgccaggta ttctctctat tcaacctcaa tacatgctgc catttgcagg
    11941 aactatgcta gttgcgattg ttgttccaat gctcttgact ttcttcttcc gcaaggctgg
    12001 tctctttaca aaaacagagg gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga
    12061 agcagaattt gtgaaccatg aaccagtaga acttacttcg gtagaaatta tcagcccact
    12121 aactggccaa gtgaaagaat tgagtcaagc gacggatcct atttttgcat caggtgtcat
    12181 ggggcaaggt ctggtcattg aaccaagcca aggtgagttg acctctccag ttaatgggac
    12241 agtgacggtt cttttcccta ccaagcatgc catcggcatt gtctctgacg agggagttga
    12301 attgctcatc cacatcggta tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct
    12361 tgtagtccaa ggagatcacg ttacagttgg tcagcaactg attcgttttg atatggatgt
    12421 cattaaggct gcaggtctgg tgacagaaac tcctgttatc atcaccaacc aagatgctta
    12481 tacagcgact attcccggaa cttatccgac aacgattcaa gctggagcat ctctcatggt
    12541 cgctacacga atctaatact cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa
    12601 cctcaaaaca gtgttttgag caacctgtgg ctagcttcct agtttgctct ttgattttca
    12661 ttgagtataa ttgaaatagc acaaataggg acggaagact gcttcctccc ttttatcaga
    12721 aaaggagaaa cagatgacac ttgataaagg aaaagtagtc tatcaaatct atccaaaatc
    12781 ttacaaagac accactgaaa acggtattgg ggatttccgt gggattatcg aaaaaattcc
    12841 ctatttagcc aaactaggcg tggatatggt ctggctcaat ccattctatc caagccctca
    12901 acgggataat ggttacgata tttcagatta tatggcagtg gatcctcttt ttggtgatat
    12961 ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac aagattgact ttatgctgga
    13021 catggtactt aatcattgtt cgacagagca tgaatggttt cagaaagccc tagctggtga
    13081 taagtattat caagactttt tcttcatcca agaccaacca acagattggc agtctaagtt
    13141 tggtggttct gcatgggcgc ctttcgggga tactgggaaa tactatcttc acctatttga
    13201 tgagacccag gctgacctta actggcgcaa ttccaatgtc cgtaaggagc ttttcaaggt
    13261 tgttaatttc tggcgcgaca agggtgtcaa aggtttccga tttgatgtga tcaatttgat
    13321 cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa gggaagccag cttatacaga
    13381 caaacccatc gttcataact atttgcgtat gatgaaccaa gccacttttg gatctgacga
    13441 tagctttatg acagttgggg aaatgtcttc taccaccatg gaaaactgtg tcctctattc
    13501 gtcgcctgat cgtcaggaat tatccatgac ctttaatttt catcacctca aggtggacta
    13561 taaggatgga caaaagtgga ccttggctcc ctttgatttt gaagagttga agagtcttta
    13621 ccatagttgg ggtaaggaaa tgagtgataa agacggttgg agtgccctct tttggaacaa
    13681 tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg
    13741 agccaccatg ctagcagcca gcattcacct gtcacgtggg acaccttata tctacatggg
    13801 tgaggaaatc gggatgattg acccagacta tgattctatg gctgattatg tggatgtcga
    13861 atcgcttaat gcctatcaga tgctcttaga agaaggaaag agtcagcaag aagccttcca
    13921 gattattcaa gctaagtcgc gtgataattc acgtattccc atgcagtggg atacttctga
    13981 aaatgcagga ttttcaacag gcactccatg gttgaaagtc ggtaaatcct ataaagatat
    14041 caatgtagaa aatgaaatca aaggtctgat tttcaccttc tatcaagact tgattcgact
    14101 gcgtaaggaa atgccaatca tttcagaagg aaattacaaa ccagcctttg aagatagcaa
    14161 acaagtctac gcttttgaac gccagtttga ggatcaaaag ttactagtgc tcaataactt
    14221 ttatgccaaa gaagtggaaa tcgacttgcc agcagtctac caaaatggac agattttgat
    14281 ttcaaactat gaagatgcag aagtgtctga gaaaattcta ctcaaaccct atcaaacact
    14341 ggctatttat gtaaattaat actcttcgaa aatctcttca aaccgtgtca gttttatctg
    14401 taacctcaaa gcggtgcttt gagcaacctg tggctagctt cctagtttgc tctttgattt
    14461 tcattgagta taaacttagc aaggagacct ctttttcaga aaagcagggt tctttttgct
    14521 gtctattcca tccttgaaag tgtagaacaa actggtcagg gtggctagtt tgtggtataa
    14581 tgaaagggat tcaaaaagaa acaggagaaa aatgatggat ttacttttag caattgtatt
    14641 gattgtgcta gcttttctag gaggagctct tggaggaatg tacttggttc gtaagcaaat
    14701 cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa gcagttcgta ctcttttgag
    14761 tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa gtttaccacc aaatcatccg
    14821 ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa taaataagga aaagtctggg
    14881 atgaaagttc cagacttctc actatgttgg ttatggttta gggatgagac tttttccttg
    14941 cattctattg atatttgatt atgattaaga gagaggtagt tgacattcta tcaagctgtc
    15001 ggtcagttcg ttcagtacaa ggaatcataa tgaacgatca atcagaaaaa agactagaaa
    15061 gaagactgta tggataatcg accaattggt tttttggatt cgggtgtcgg gggcttgacc
    15121 gttgtgcgcg agctcatgcg ccagcttccc catgaagaaa tcgtctatat tggagattcg
    15181 gcgcgggcgc cctatggccc ccgtcctgct gagcaaattc gtgaatatac ttggcagctg
    15241 gtcaactttc tcttgaccaa ggatgtcaaa atgattgtca ttgcttgtaa cactgcgact
    15301 gcggtcgtct gggaagaaat caaggctcaa ctagatattc ctgtcttggg tgtaattttg
    15361 ccaggagctt cggcagccat caagtccagt caaggtggga aaatcggagt gattggaacg
    15421 cccatgacgg tacaatcaga catataccgt cagaaaatcc atgatctgga tcccgactta
    15481 caggtggaga gcttggcctg tcccaagttt gctcccttgg ttgagtcagg tgccctgtca
    15541 accagtgtta ccaagaaggt ggtctatgaa accctgcgtc ccttggttgg aaaggtggat
    15601 agcctgattt tgggctgtac tcattatcca ctccttcgcc ctattatcca aaatgtgatg
    15661 gggccaaagg ttcagctcat cgatagtggg gcagagtgcg tacgggatat ctcagtctta
    15721 ctcaattatt ttgaaatcaa tcgtggtcgc gatgctggac cactccatca ccgtttttac
    15781 acaacagcca gtagccaaag ttttgcacaa attggtgaag aatggctgga aaaagagatt
    15841 catgtggagc atgtagaatt atgacaaata aaatttatga atataaggat gaccaggact
    15901 ggtatgttgg gtcttatagt atttttggtg gcgttaacag tttgagcgac tataagacag
    15961 attttcctct gtttgaattc tccaaaatat ttggagatga agagtatggt ttcccgcttt
    16021 cagttactgt tttacgctat ggttctacct accgtttgtt ctcctttgtg gtagacatgc
    16081 ttaatcaaga aatgggacga aacttggaag ttattcaacg tcatggggcc ctgctcttgg
    16141 ttgaaaatgg gcaactcttg tatgtagaat tgcctaaaga aggggtcaat gttcatgatt
    16201 tctttgagac aagcaaggtc agagaaacct tgttgattgc gactcgtaac gaaggtaaaa
    16261 ccaaggaatt ccgagctatc tttgataagt taggctacga tgtggaaaat cttaatgact
    16321 accctgacct gcctgaagta gcagaaacag gtatgacctt tgaagaaaat gcccgcctta
    16381 aggcagaaac catttctcaa ttaacgggca agatggtttt ggcagatgat tctggtctca
    16441 aagtcgatgt ccttggtggc ttaccaggcg tctggtcagc tcgtttcgca ggtgtgggag
    16501 caactgaccg tgaaaataat gccaaactct tgcacgaatt ggccatggtc tttgaactca
    16561 aggaccgctc ggctcagttc cacacaaccc tagtcgtagc cagcccaaat aaggaaagtt
    16621 tagttgttga agcagactgg tcaggttata ttaactttga acctaagggt gaaaatggct
    16681 ttggctatga tcccctcttc cttgtaggag agacaggtga gtcatcagct gaattaaccc
    16741 tagaagaaaa aaatagtcaa tctcaccgtg ccttagccgt taagaaactt ttggaggtat
    16801 ttccatcatg gcaaagcaaa ccatcattgt aatgagcgat tcccatggcg atagcttgat
    16861 tgtggaagaa gtccgtgatc gctatgtggg caaagtcgat gccgtttttc ataacggcga
    16921 ttctgaacta cgtccggatt ctccactttg ggagggcatc cgcgttgtta aagggaacat
    16981 ggacttctac gccggctacc cagaacgtct ggtgactgag cttggttcga ccaagattat
    17041 ccaaactcat ggtcacttgt ttgacatcaa tttcaacttt caaaagttgg actactgggc
    17101 tcaggaggaa gaggccgcta tctgcctcta tggtcacttg catgtgccaa gtgcttggtt
    17161 ggaaggcaag atcctctttc taaatccagg ttctatcagt caaccacgag gtaccatcag
    17221 agaatgtctc tatgctcgtg tggagattga tgatagttac ttcaaagtgg actttttgac
    17281 acgagatcac gaggtgtatc caggtttgtc caaggagttt agccgatgat tgccaaggag
    17341 tttgagactt tcttgttggg gcaggaggaa acttttttga cccctgctaa aaatctagct
    17401 gtgttgattg atacccacaa tgcggatcat gcgaccctct tgctcagtca gatgacctat
    17461 acccgtgttc ccgttgtgac agatgaaaaa cagtttgttg ggacgattgg actcagagat
    17521 attatggctt atcagatgga gcatgacttg agccaagaaa tcatggcgga tacggatatc
    17581 gttcatatga caaaaacgga cgtagcggtt gtttcgcctg atttcaccat tacggaggtc
    17641 ttgcacaagc tagtagatga gtccttctta ccggtcgtgg atgcagaggg tattttccaa
    17701 gggattatta cgcgcaagtc catcctcaag gccgttaatg ccctcttgca tgactttagt
    17761 aaggaatatg agattcgatg ccaatgagag acaggatttc agccttttta gaggaaaagc
    17821 agggcttgtc tgtcaattcc aagcagtcct ataagtatga tttggagcaa tttttagaca
    17881 tggtaggtga gcggatttct gagaccagtc tcaagattta ccaagcccag ctagccaatc
    17941 taaaaatcag cgcccagaag cgaaagattt cggcctgtaa ccaatttcta tactttctct
    18001 atcaaaaagg agaggtggac agcttttacc gcttggaatt agccaaacaa gctgaaaaga
    18061 agacggaaaa gccagagatt ctatacctag actctttttg gcaggaaagc gaccatccag
    18121 agggccgctt gctagcgctc ttaatcctag aaatggggct cttgcccagt gagattttag
    18181 ccatcaaggt tgcggacatc aatctggatt ttcaggtgct gcgaatcagc aaggcttccc
    18241 aacagaggat tgtcaccatt cccacggcct tgctttcaga attggaaccc ttgatggggc
    18301 agacctatct ttttgaaaga ggagagaaac cctattctcg tcagtgggcc tttcgtcagt
    18361 tagaatcttt tgtcaaggag aaaggttttc catccttatc agctcaagtc ttacgtgaac
    18421 agtttattct aagacaaata gaaaacaagg tcgatttgta cgaaattgca aaaaaattag
    18481 gattaaaaac agtcctgacc ttagaaaaat atagataatg gatattaaat taaaagattt
    18541 tgaaggaccc ctggacttgc tcttgcatct ggtttctaag taccagatgg atatctacga
    18601 tgtgcccatt acggaagtca tcgaacagta tctagcctat gtctcaaccc tgcaggccat
    18661 gcgtctggaa gtgacgggtg agtacatggt catggctagt cagctcatgc tgattaagag
    18721 tcgtaaactc cttccgaagg tagcagaagt gacagacttg ggggatgacc tggagcagga
    18781 cctcctctct caaatcgaag aatatcgcaa gttcaagctc ttgggtgagc acttggaagc
    18841 caagcaccaa gaacgggccc agtattattc caaagcgccg acagagttga tttacgaaga
    18901 tgcggagctt gtgcatgaca agacgaccat tgacctcttt ttggcttttt caaatatcct
    18961 agccaagaaa aaagaggagt ttgcacaaaa tcacacgacg atcttgcggg atgagtataa
    19021 gattgaggac atgatgatta tcgtgaaaga gtccttgatt ggacgagatc aattgcgctt
    19081 gcaggatttg ttcaaggaag cccagaatgt ccaagaggtc atcaccctct ttttggcaac
    19141 cctagagtta atcaaaaccc aggagctgat cctcgtgcaa gaggagagtt ttggagatat
    19201 ctatctcatg gaaaagaagg aagaaagtca agtgcctcaa agctagactt gatagagagg
    19261 aaagatgagt actttagcaa aaatagaagc gctcttgttt gtagcgggtg aagatgggat
    19321 tcgggtccgc cagttagctg aactcctctc tctgccaccg acaggcatcc agcaaagttt
    19381 aggaaaatta gcccagaagt atgaaaagga cccagattcc agtttggctt tgattgagac
    19441 aagtggtgct tatagattgg tgaccaagcc tcaatttgca gagattttga aggaatactc
    19501 taaggcgcct atcaaccaga gcttgtctcg ggctgccctt gagaccttgt ccattattgc
    19561 ctacaaacag ccgattacgc ggatagaaat tgatgccatc cgtggagtta actcgagtgg
    19621 agccttggca aagttgcagg cttttgacct gataaaggaa gacgggaaaa aggaagtatt
    19681 ggggcgcccc aacctctatg tgactacgga ttatttccta gattacatgg ggataaacca
    19741 tttagaagaa ttaccagtga ttgatgagct tgagattcaa gcccaagaaa gccaattatt
    19801 tggtgaaagg atagaagaag atgagaatca ataagtatat tgcccacgca ggtgtggcca
    19861 gtaggagaaa agcagaagag ctgattaagc aaggcttggt gacggttaac ggccaagtgg
    19921 tgcgtgaact agcaaccact atcaagtcag gcgacaaggt cgaagttgaa ggtcaaccta
    19981 tctacaacga agaaaaggtc tactatctgc ttaacaaacc acgcggtgtg atttccagtg
    20041 tgacagatga taagggtcgc aagacggttg tcgacctctt gcccaatgtc aaagagcgta
    20101 tttaccctgt gggtcgtttg gactgggata catcaggtgt cttgattttg accaatgatg
    20161 gggactttac agacgagatg attcaccctc gtaatgagat tgacaaggtt tatgtcgcgc
    20221 gtgttaaagg tgtggccaat aaggacaatc tccgcccctt gacccgtggt cttgagattg
    20281 atggtaagaa aaccaagcca gctgtttatg aaattctcaa agtggaccca gtcaaaaatc
    20341 gctctgtggt gcagttgacc atccatgaag ggcgtaacca tcaggttaaa aagatgtttg
    20401 aagctgttgg tctccaagta gataagttgt ctcggactcg tttcggacac ctagacttga
    20461 caggactccg tccaggagaa tcccgtcgtc ttaataaaaa agaaatcagc caactacaca
    20521 ccatggctgt aactaagaaa taatgaaacg aattttaata gcgcctgtgc gcttttacca
    20581 acgttttatc tcaccagtct ttccaccctc ttgtcgcttt gagctgactt gttccaacta
    20641 catgattcag gctattgaaa aacatggttt taagggggta ttgatgggct tggctcggat
    20701 tttacgttgt catccctggt cgaaaacagg taaggacccc gttccagacc acttttccct
    20761 taaacgaaat caagaagggg aatgaggtgg ggtaaataga tttcaaaatg ataaaaacgc
    20821 atcctatcag gtttgagtaa acttgatagg atgcgtttta gaatgtcaaa attttatact
    20881 cttcgaaaat ctcttcaaac cgcgtcagct ttcatctgca acctcaaaac agtgttttga
    20941 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt aaattgagtt
    21001 tgaagtggct tatttcaaag ctttttgtat gtcttcaatc atgagttttg ttgattcaag
    21061 tccgcctccg cttagatacc agaggtctgg tgttagttgg ataatcttac catttttagc
    21121 agcaggtgtt tcagcgataa gggcattttc taggacaccg tcgttgctag agttgtcccc
    21181 accgatggca agggtacggt tgatgacaaa gaggatgtca gggttgattt ctttgacact
    21241 ttcaaagctg acttcttgtc cgtggcgtga gtcttcaaat tttgtatcag ttggtttgaa
    21301 tttcaaggtt tggtacaaga aagagaaacg agatttggca ccaaaggctg ccatttttcc
    21361 ttcattaagg aggatcgcaa gggctttttt gtcagagctt tcatttttag tagcgacttc
    21421 ttggatgctc ttgtctagct tggccaattc ttccttggct ttctgtgtac cagtttcgcc
    21481 gaaggcactt gctaaggatt cgatattagc cttggtagaa gtccagtagt cgtccttgct
    21541 tgcttggaag agaacggttg gggcgatttc tttgaatttg tctacgaatt tttgtgtacg
    21601 tggtgaagcg ataatcaaat caggctcaag ggcggcgata gcttctaaat caggttcttt
    21661 catagaacca acatttttga cagttcccac taggtctttt agataagtcg gaacagtttt
    21721 tgtaggcatt ccgacgatat ttttttcaaa tcctaaagcg cgaatagtat ccgcagcgcc
    21781 gaggtcaaag gtcacaatct tttcaggaac tttggaaagt ttgacctcgg ccagtgaact
    21841 tttaatggtt acctctgttg gagcagagct actggtctct gtctgactag tgcttgagtt
    21901 tgtactacat gcaccaagta ggagcaagaa gctggccact agggcagtga aataaagttt
    21961 aagggatgtt ttcataattt ctcctcttta aaatgtgata acgatttagg gagtctcttg
    22021 atcttattga ctaagagact gaaggttctc taacttgagc ttttatgtta ctagctatag
    22081 atacagatct ttttgtcatt gatatcagct agcgtgatgg gaatctcata aagttgactg
    22141 agcaggtcag cctgcatgat ttgatcggtt cttcccttgc taaagacctg gccgtccttg
    22201 aaggcgacaa tttcatctgc atactgactg gctatgttga tatcgtggag gacgatgata
    22261 atggtcttgc cgagttcctc caccagtcgt cgaagaatct gcatcatgct gacgctttgc
    22321 ttgatatcga gattgttgag tggttcgtcc agcaagataa agtccgtatc ctgggccagt
    22381 accatagcga taaagacgcg ctggagttgc ccccctgaca ggctattgat gtagcggtct
    22441 tttaagttgg tcagttccaa atagttcaga gtttctcgga ttttttccca gtcttctgat
    22501 ctaagtcgac ctcggctgta gggaaaacgt ccaaaactga ccagttcttc aacagtcaat
    22561 ttggcttggt aattgatttt ctgttttagg atggttagtt cttgggccag ttcttgcgaa
    22621 ttccagctct cgatttcacg tcctttgata ctgagaactc cctgatcttt cttggttagt
    22681 ctgctcatga tggagaggag agtcgatttt ccagcaccat ttggaccaat aaaggctgtc
    22741 agtttttgag gactgacttc aagcgaaatg ccttgcaaaa tatcctgttt ttgaatggat
    22801 ttgtcaatgt tttccagttt cactgacgag ccctcctata tagtaagata aagaataaga
    22861 agccacccac actctcaatg atcatactga tacgaatttc cagtgcaaag actcgttcaa
    22921 tcaaggcttg ccccaaggtt aagctaataa atccaaccag aatggccact ataaagagta
    22981 acttgtgctg atagtctttg acaatcaggt aggtgaggtt ggccagcata aagccgaaga
    23041 agaccatagg tcctaccaag gcagtggccg ttgaggtcaa aagcacgatt ccccagagga
    23101 gctctttctg ttctttttca acatcgagtc ccaatatctg agccgtttct ctttgcaggt
    23161 gcaagacatc tagaacgact gcttttcgaa agaaaaagat tgtcaaagcg aggatgatca
    23221 gagaaccgat ggctaggatg gaagtgttaa gatgttgaaa ggaggcaaaa agactatttt
    23281 gcagtttatc gtattcgttt ggatccatta ggacttgaag gaaggtgctg atatttcgaa
    23341 agagacttct gagcgctaga cagatcagca ggatgaagac caggtcttgc ttcatcagtg
    23401 tcttcaagta accttgtaag gcgagaaaga agagggactg gacaagaagt aagactagga
    23461 attctaagat aggggatttg ccaagttgaa gaaacttgct ttcaaaaacc agtagtaggg
    23521 tttgtagtag gacgtagaag gattcaattc ccaaaatact aggcgtcagg aagcgatttt
    23581 ccgtcagggt ttgaaaacta atggtcgaaa tcccagtcgc gatggctacc aagagataaa
    23641 cgatgatctt ttgggaacgc aacttccaag caaaggctga caagtgagtg atgggccaaa
    23701 agtagagaag acaagctccg atggcaagaa taatgagaat ccagaagagc ttggtatgtt
    23761 tgcttttagt ctgcatcttt tcgtccccct ctccagagaa gtaggataaa gacgagacta
    23821 ccgatgattc ctagcaagag actgacagac aactcatagg gcctaatcag aactcgggat
    23881 aggatatcgc aagccagaac tagattggca ccaaccagtg cgaccatgag tttggtttga
    23941 cttagattat ctccatagcg cttgcgaaca agattgggaa cgataactcc gagaaatggt
    24001 aagccaccca cggtaatcat ggtgacgctt gtcgttagcg ccaccagaaa gagggccagt
    24061 ttttcaagta gggagtagga aatccccaaa ctctcgctgg tttctttccc tagattcatg
    24121 atggtgaagg tttgggataa tttccaaacg gttatcagga tgatgaggcc taagaagagc
    24181 cactcatact gatgggtctg aatcatggag aaggagccct gggtccaggc agtcatactc
    24241 tgaaccagat tgaaacgata ggcgataact tctgtgactg agccgataat cccgctatag
    24301 atgatcccaa tcagaggcaa catccacctt tcctttacag taaaaatggt cataaaggct
    24361 aggaagaaga gggtgaatag aatggatgaa acaaaagcga agagcatctt ttgggtcaga
    24421 ctagccgatg gaaagacaaa aaggctcagc accattccca gtttggcggc ttcagtcgtt
    24481 ccaactgtac tcggtgcagc aaactgattt tgggtaatag tctgcatgag aaggcctgcc
    24541 atactcatac tagaggcagt caggagaata ctgatagttc ttgggagacg ggactcttga
    24601 aagaggagcc aggtctgctg gtcgaaatca aatagctttc cccatgaaaa atcactggtc
    24661 ccaatgctaa tagagagaaa gactaggagt agaagtaagc caattaaata atgagaaagt
    24721 ttcatacccc gtcctttcat gtagatttgg tatcgaaaga tacctgcgga tataaatgta
    24781 acattatttt tctaatctgt caataaaatt tctgacaatt taataaatac aacaaggaga
    24841 gagcaacaag actttctcct ttgttatcct attctaaaat gtttttacct taatctgata
    24901 aaataatatc ttcgagggag tagctagccg tccaatcaag atattgttta gcttttgaag
    24961 catctgctag gacactggct gggtcactag cacgtcgagc aacaatctcg tgtgggattt
    25021 tttaatttag taattcttca gcagttttaa agatttcttt gatagtatag ccttttttag
    25081 ttcctaagtt aaagatttga gaagaactgt cttcttgaaa taggtagttc attcctttaa
    25141 catgagccta tgcaaggtcc aagacataaa tgtaatctcg aatacatgaa ccgtcacgtg
    25201 tatcgtagtc atctccaaat atttttaagc tatcattttg tcccaatgcg gtcttgttga
    25261 tatttggaat gatgtgagtt ggatttttca cacgcagacc gtttgaagca tccatttcag
    25321 ccccagcaac attaaagtaa cggaaaataa catatttcca gtcgtagcga ttggccatcc
    25381 agtaaatcat tcgttcgccc atcagttttg tctctgcata agggttgaca gggtcgagca
    25441 gggtatcttc agtcaccggc ttgtcaatac agttatttcc atagagagaa gcagtcgaag
    25501 agaacatgat tttttgaatg ccaacttcag ataagacttt gagaacttgg ttcataccag
    25561 caacgttggc agtgaagtat ttacttggat tttcaatact ttcgctcaca acaatttcac
    25621 ctgcacaatg gaggacagca tcaatctgat tttcttctaa gtaagccttt aaggcgctag
    25681 catcataaac atccagttct ttaaagctag cacgactatc cacagccgct cgatttcctg
    25741 tggagagatt atcgaggaca tgcacctgat agccagcatt taaaagagct ttaacggtat
    25801 gggagccaat gtagctagtc ccacctgtaa caagaattgt tttggtcatg attttttcct
    25861 tttctagttt tg
//
LOCUS       DAVLFB010000020        25182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLFB010000020 DAVLFB010000000
VERSION     DAVLFB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.020"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..467
                     /locus_tag="VXD58_000740"
     CDS             <1..467
                     /locus_tag="VXD58_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0675482.1"
                     /translation="YFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREALITR
                     EKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEV
                     SLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            505..810
                     /locus_tag="VXD58_000741"
     CDS             505..810
                     /locus_tag="VXD58_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0675483.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            858..2333
                     /locus_tag="VXD58_000742"
     CDS             858..2333
                     /locus_tag="VXD58_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV0675484.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQEVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2683..9384
                     /locus_tag="VXD58_000743"
     CDS             2683..9384
                     /locus_tag="VXD58_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV0675485.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9632..10488)
                     /locus_tag="VXD58_000744"
                     /pseudo
     CDS             complement(9632..10488)
                     /locus_tag="VXD58_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10699..11058)
                     /locus_tag="VXD58_000745"
     CDS             complement(10699..11058)
                     /locus_tag="VXD58_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV0675486.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11150..12106
                     /locus_tag="VXD58_000746"
                     /pseudo
     CDS             11150..12106
                     /locus_tag="VXD58_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12090..12647
                     /locus_tag="VXD58_000747"
     CDS             12090..12647
                     /locus_tag="VXD58_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0675487.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12640..12894
                     /locus_tag="VXD58_000748"
     CDS             12640..12894
                     /locus_tag="VXD58_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675488.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12906..14960
                     /locus_tag="VXD58_000749"
     CDS             12906..14960
                     /locus_tag="VXD58_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV0675489.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15139..15999
                     /locus_tag="VXD58_000750"
     CDS             15139..15999
                     /locus_tag="VXD58_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV0675490.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            16210..16920
                     /gene="ccdA2"
                     /locus_tag="VXD58_000751"
     CDS             16210..16920
                     /gene="ccdA2"
                     /locus_tag="VXD58_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0675491.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYILKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16940..17506
                     /locus_tag="VXD58_000752"
     CDS             16940..17506
                     /locus_tag="VXD58_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV0675492.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTTSKT
                     SAAKGKEVTDFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17517..18629
                     /gene="msrB"
                     /locus_tag="VXD58_000753"
     CDS             17517..18629
                     /gene="msrB"
                     /locus_tag="VXD58_000753"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV0675493.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18852..19589
                     /locus_tag="VXD58_000754"
     CDS             18852..19589
                     /locus_tag="VXD58_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675494.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19586..21277
                     /locus_tag="VXD58_000755"
     CDS             19586..21277
                     /locus_tag="VXD58_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0675495.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21393..21956
                     /locus_tag="VXD58_000756"
     CDS             21393..21956
                     /locus_tag="VXD58_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV0675496.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            21978..>25182
                     /locus_tag="VXD58_000757"
     CDS             21978..>25182
                     /locus_tag="VXD58_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0675497.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEKGHVGEALAQEQLPEYKVTEGTLVETSTTDLDYKTET
                     TEDPTKYTDEETVLRTGEKGSQVTKTTYKTVEGVKTDQVLSTSTEVTKEPVNQQVIRG
                     TKPIEGTLIEESLEKIPFKEVVKEDDQLKKGLEVVAQEGKEGQKKITKTFNTIKGVKT
                     EDAPKVTEEILEEPQDKILKRGTKSFEKPVLTITEIESKDLKRTSDVKYSLENPSKAA
                     IKSITLTLKKGDEIVKTLNVSPEDLTTTLTDLQYYKDYKLETKMVYDRGEGDEEEVLK
                     EEPLRIDLKKVEIKDIIRTDLIKYENQVETDETRLTSVPTDLSNYYLKVTSNDHKTNL
                     LAVQKIEESTIDGKQVYKVIAVADNLVQRNVDNRFEETYTYYIEKPKASQAGIFYDFE
                     ELIQAIQTNPAGEFKLGQSMSARNVVPKGKSYITSEFTGKLLSDGDKRYAIHNLEHPL
                     FDKIKAGTVKNINFENIDIDLSNQDNIAAIANEISGTTRIENIKAIGTITARNNVAGF
                     VNNIKEDATLENVAFIGSINSAGNNSTVGGIAGSNYMGFVNRAYVDATITANNANASM
                     LVPFVQYKAHSWLSGTKGKLTNSVAKGILDVKNTKYVGGITSKTWPYGAVQDNVTYAK
                     VIKGQEIFGSNDVNDGDANPFVSNLFGVIGYSSSEDGTGKDNKNKNKLKHLSKSEADA
                     KVASFNITAAKFVSEPYELNKLNNVSSREAAYESIQDY"
BASE COUNT         7802 a         4713 c         5533 g         7134 t
ORIGIN      
        1 attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa gaacatctct
       61 ttgatcaggt tgcaacttta ttggaagaac gagaaattgt aactccaact tatcgtgaag
      121 ccttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt ctaggaaagg
      181 acttgccaaa tgtagcgatt cctcacacag atattgttca taatctagct gagaaagtgg
      241 tggttgttcg attagaaaaa ccagtaactt ttcacaatat gatagctcct gataaggaag
      301 tagaagtatc tctactcttc tttatcatta acaattcaag ttcaagtcaa acgaacattc
      361 tggctcagtt gatggacttt ttcacaggaa atggtcatct tgaagaccta tcaaaaattt
      421 ctgaaccaga aaaactttat gcttacattg ctgaagcaac cgcttaatct tgtctattaa
      481 aaaataaaat cggaggaaat ccaaatgatt aaaattcttg ctgcctgcgg tgcaggtgtt
      541 aactcaagtc accaaattaa aagtgctcta gaagaagaac tttcaaaccg tggttatgat
      601 gttcactgtg atgcagtcat ggtgaaagat gtaaacgaag accttatgaa aggttacgat
      661 atctttacac caatcgctgc aacagacctt ggttttgaac caggtatccc agttatcgaa
      721 gctgggccaa tcttattccg tatcccagca atgagcgctc cagtatttga caatattgaa
      781 gcagctatta aagaacatgg attaagctaa ttctaatttg ttaacattca tataacataa
      841 aaaagggagg aactgttatg gatgcaatct ttgacctaat cggaaaggtt ttcaatccca
      901 tcttagaaat gggtggacct gtcatcatgt tgatcatttt gacagtattg gctttacttt
      961 ttggagtgaa attctccaaa gcgcttgaag gtggtatcaa acttgccatc gctcttacag
     1021 gtatcggtgc tatcatcggt atgctaaatg gtgctttctc agcatcactt gcaaaattcg
     1081 ttgaaaacac tggtatccaa ttgagtatta cagacgttgg ttgggcacca ctcgcaacaa
     1141 tcacttgggg ttctgcttgg acactatact tcttgctcat catgttgatt gtcaacgtag
     1201 tgatgcttgc tatgaagaaa accgatacac ttgatgtcga tatctttgat atctggcact
     1261 tgtctatcac aggtctcttg attaaatggt atgctgataa caatggtgtg agtcaagagg
     1321 tttcactctt tattgctaca gcagctatcg tccttgtcgg tgtgttgaaa attatcaact
     1381 ctgacttgat gaaacctaca tttgatgacc ttcttaacgc cccaagttca tcaccaatga
     1441 catcaactca catgaactac atgatgaacc cagttatcat ggttttggat aagatttttg
     1501 aaaaattctt cccaggcctt gataaatatg actttgatgc tgctaaattg aacaagaaaa
     1561 tcggtttctg gggatctaaa ttcttcatcg gtttcatcct tggtatcgtt atcggtatta
     1621 tgggaactcc acatccaatt gcaggtgttg cagatgcaga taaatggcgt cttgttatca
     1681 aaggatggtt gtctcttggt ttgactgccg gtgtatcttt ggaactcttc tcacttatcg
     1741 gttcatggtt catcgcagcc gtagaaccac tatcacaagg tattacaaac gttgctacta
     1801 aacgtcttcg aggacgtaaa ttcaatatcg gtcttgactg gccattcatc gctggtcgtg
     1861 ctgaaatctg ggcttgtgcc aacgtacttg caccaatcat gttgattgaa gcagtgcttc
     1921 tttcaaaagt tggaaatggt atcttgccac ttgcaggtat catcgctatg ggtgttactc
     1981 cagctctctt ggttgtaact cgtggtaaat tgctccgtat gattatcttc ggaacactct
     2041 tgttgccact cttcctcctt tcaggtacac ttattgcacc atttgcaaca gaacttgcta
     2101 aaggtgtagg tgccttccca gaaggtgtga gccaaactca attgattact cactctactc
     2161 ttgaaggacc aatcgaaaaa cttcttggtt ggacaattgg taacactaca actggtgata
     2221 tcaaagcaat ccttggtgca gtagtcttcc ttgtattcta tatcggtatc tttgcttggt
     2281 acagaaaaca aatgatcaaa cgtaacgaag agtacgcagc aaaagcaaaa taatgcgctc
     2341 ctataaaata taaactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc
     2401 taaaaacaca gttttgaggt tgtagataag actgacgaag tcagctcaaa acatggtttt
     2461 gaggttgtag atgaaactga cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa
     2521 actgacgaag tcagtaatca tatctacggc aaggcgacgc tgacgaagtt tgaattggat
     2581 tttcgcagag tgtaaattgt tgaaactagg ttgtcatacc atgtatacca cttgggcttt
     2641 ggtagtagct aactgcacta aatataatat aaggagggtt ttatggggaa aggccattgg
     2701 aatcggaaaa gagtttatag cattcgtaag tttgctgtgg gagcttgctc agtaatgatt
     2761 gggacttgtg cagttctatt aggaggaaat atagctggag aatctgtagt ttatgcggat
     2821 gaaacactta ttactcatac tgctgagaaa cctaaagagg aaaaaatgat agtagaagaa
     2881 aaggctgata aagctttgga aactaaaaat gtagttgaaa ggacagaaca aagtgaacct
     2941 agttcaactg aggctattgc atctgagaag aaagaagatg aagccgtaac tccaaaagag
     3001 gaaaaagtgt ctgctaaacc ggaagaaaaa gctccaagga tagaatcaca agcttcaagt
     3061 caagaaaaac cgctcaagga agatgctaaa gctgtaacaa atgaagaaat gaatcaaatg
     3121 attgaagaca ggaaagtgga ttttaatcaa aattggcact ttaaactcaa tgcaaattct
     3181 aaggaagcca ttaaacctga tgcagacgta tctacgtgga aaaaattaga tttaccgtat
     3241 gactggagta tctttaacaa tttcgatcat gaatctcctg cacaaaatga aggtggacaa
     3301 ctcaacggtg gggaagcttg gtatcgcaag actttcaaac tagatgaaaa agacctcaag
     3361 aaaaatgttc gccttacttt tgatggcgtc tacatggatt ctcaagttta tgtcaatggt
     3421 cagttagtgg ggcattatcc aaatggttat aaccagttct catatgatat caccaaatac
     3481 cttcacaaag atggtcgtga gaatgtgatt gctgtccatg cagtcaacaa acagccaagt
     3541 agccgttggt attcaggaag tggtatctat cgtgatgtga ctttacaagt gacagataag
     3601 gtgcatgttg agaaaaatgg gacaactatt ttaacaccaa aacttgaaga acaacaacat
     3661 ggcaaggttg aaactcatgt gaccagcaaa atcgtcaata cggacgacaa agaccatgaa
     3721 cttgtagccg aatatcaaat cgttgaacga ggtggtcatg ctgtaacagg cttagttcgt
     3781 acagcgagtc gtaccttaaa agcacatgaa tcaacaagcc tagatgcgat tttagaagtt
     3841 gaaagaccaa aactctggac cgttttaaat gacaaacctg ccttgtacga attgattacg
     3901 cgtgtttacc gtgacggtca attggttgat gctaagaagg atttgtttgg ttaccgttac
     3961 tatcactgga ctccaaatga aggtttctct ttgaatggtg aacgtattaa attccatgga
     4021 gtatccttgc accacgacca tggggcgctt ggagcagaag aaaactataa agcagaatat
     4081 cgccgtctca aacaaatgaa ggagatggga gttaactcta tccgtacaac ccacaaccct
     4141 gctagtgagc aaaccttgca aatcgcagca gaactaggtt tactcgttca ggaagaggcc
     4201 tttgatacgt ggtatggtgg caagaaacct tatgactatg gacgtttctt tgaaaaagat
     4261 gccactcacc cagaagctcg aaaaggtgaa aaatggtctg attttgacct acgtaccatg
     4321 gtcgaaagag gcaaaaacaa ccctgctatc ttcatgtggt caattggtaa tgaaataggt
     4381 gaagctaatg gtgatgccca ctctttagca actgttaaac gtttggtcaa ggttatcaag
     4441 gatgttgata agactcgcta tgttaccatg ggagcagata aattccgttt cggcaatggt
     4501 agcggagggc atgagaaaat tgctgatgaa ctcgatgctg ttggatttaa ctattctgaa
     4561 gataattaca aagcccttag agctaagcat ccaaaatggt tgatttacgg ttcagaaaca
     4621 tcatcagcaa cccgtacacg aggaagttac tatcgccctg aacgtgaatt gaaacatagc
     4681 aatggacctg agcgtaatta tgaacagtca gattatggaa atgatcgtgt gggttggggg
     4741 aaaacagcaa ccgcttcatg gacttttgac cgtgacaacg ctggctatgc tggacagttt
     4801 atctggacag gtacggacta tattggtgaa cctacaccat ggcacaacca aaatcaaact
     4861 cctgttaaga gctcttactt tggtatcgta gatacagccg gcattccaaa acatgacttc
     4921 tatctctacc aaagccaatg ggtttctgtt aagaagaaac cgatggtaca ccttcttcct
     4981 cactggaact gggaaaacaa agaattagca tccaaagtag ctgactcaga aggtaagatt
     5041 ccagttcgtg cttattcgaa tgcttctagt gtagaattgt tcttgaatgg aaaatctctt
     5101 ggtcttaaga ctttcaataa aaaacaaacc agcgatgggc ggacttacca agaaggtgca
     5161 aatgctaatg aactttatct tgaatggaaa gttgcctatc aaccaggtac cttggaagca
     5221 attgctcgtg atgaatctgg caaggaaatt gctcgagata agattacgac tgctggtaag
     5281 ccagcggcag ttcgtcttat taaggaagac catgcgattg cagcagatgg aaaagacttg
     5341 acttacatct actatgaaat tgttgacagc caggggaatg tggttccaac tgctaataat
     5401 ctggttcgct tccaattgca tggccaaggt caactggtcg gtgtagataa cggagaacaa
     5461 gccagccgtg aacgctataa ggcgcaagca gatggttctt ggattcgtaa agcatttaat
     5521 ggtaaaggtg ttgccattgt caaatcaact gaacaagcag ggaaattcac ccttactgct
     5581 cactctgatc tcttgaaatc tagtcaagtc actgtcttta ctggtaagaa agaaggacaa
     5641 gaaaagactg ttttggggac agaggtgcca aaagtacaga ccattattgg agaggcacct
     5701 gaaatgccta ccactgttcc gtttctatac agtgatggta gtcgtgcaga acgtcctgta
     5761 acctggtctt cagtagatgt gagcaagcct ggtattgtaa cggtgaaagg tatggctgac
     5821 ggacgagaag tagaagctcg tgtagaagtg attgctctta aatcagagct accagttgtg
     5881 aaacgtattg ctccaaatac tgacttgaat tctgtagaca aatctgtttc ctatgttttg
     5941 actgatggaa gtgtacaaga gtacgaagta gatagctggg agattacgga agtagataaa
     6001 gccaaacttt cagtagctgg atcacgtatt caaatgactg gtcagttagc tggagaaact
     6061 attcatgcaa cccttgtggt agaagaagga aatgctgcag cacctgtagt gccaactgtt
     6121 actgttggag gtgaagcagt aacaggtctt actagtcgac aaccaatgca atatcgtact
     6181 ctatcttatg gtgcccaatt gccagaagtc acagcaagtg ctgaaaatgc tgatgtgaca
     6241 gttcttcaag caagcgcagc aaacggcatg cgtgcaagca tatttgttca gcctaaagat
     6301 ggtggccctc ttcaaaccta tgcaattcaa ttcctagaag aagcaccaaa aattgctcac
     6361 ttgagcttgc aagtggaaaa agctgacagt ctcaaagaag accaaactgt caaattgtcg
     6421 gttcgagctc actatcaaga tggaacgcaa gctgtattac cagctgataa agtaaccttc
     6481 tctacaagtg gtgaagggga agtcgcaatt cgtaaaggaa tgcttgagtt gcataagcca
     6541 ggagcagtca ctctcaaagc ggaatatgag ggagctaaag gccaagttga actcactatc
     6601 caagccaata ctgagaagaa gattgcgcaa tctatccgtc ctgtaaatgt agtcacagat
     6661 ttgcatcaaa aacctactct tccaacaaca gtaacggttg agtatgacaa aggtttccct
     6721 aaagctcaca aagtcacttg gcaagctatt ccgaaagaaa aactagactc ctatcaaaca
     6781 tttgaagtac taggtaaagt tgaaggaatt gaccttgaag cgcgtgcaaa agtctctgta
     6841 gaaggtatcg tttcagttga agaagtcagt gtgacaactc caatcgcaga agcaccacaa
     6901 ttaccagaaa gtgttcggac atatgattca aatggtcacg tttcatcagc taaggttgca
     6961 tgggatgcga ttcgtccaga gcaatacgct aaggaaggtg tctttacagt taatggtcgc
     7021 ttagaaggta ctcaattaac aactaaactt catgttcgcg tatctgctca aactgagcaa
     7081 ggtgcaaaca tttctgacca atggaccggt tcagaattgc cacttgcctt tgcttcagac
     7141 tcaaatccaa gcgacccagt ttcaaatgtt aatgacaagc tcatttccta caataaccaa
     7201 ccagccaatc gttggacaaa ctggaatcgt actaatccag aagcttcagt cggtgtccta
     7261 ttcggagatt caggtatttt gagcaaacgt tcagttgata acttaagcgt tggcttccat
     7321 gaagaccatg gagttggtgc accgaagtct tatgtgattg agtattatgt tggtaagact
     7381 gtcccaacag ctcctaaaaa ccctagtttt gttggtaatg aggaccatgt ctttaatgat
     7441 tctgccaact ggaaaccagt tactaatcta aaagcccctg ctcaactcaa ggctggagaa
     7501 atgaaccact ttagctttga taaagttgaa acctatgctg ttcgtattcg catggtgaga
     7561 gcagacaaca aactaggaac gtctatcaca gaggtacaaa tctttgcgaa acaagttgcg
     7621 gcagccaagc aaggacaaac aagaatccaa gttgacggta aagacttagc aaacttcaac
     7681 cctgatttga cagactacta ccttgagtct gtagatggaa aagttacggc agtcacagca
     7741 agtgttagca acaatggcct cgctaccgtc gttccaagcg ttcgtgaagg tgagccggtt
     7801 cgtgtcatcg cgaaagctga aaatggcgac atcttaggag aataccgtct gcacttcact
     7861 aaggataaga acttactttc tcataaacca gttgctgcgg ttaaacaagc tcgcttgcta
     7921 caagtaggtc aagcacttga attgccgact aaggttccag tttacttcac aggtaaagac
     7981 ggctacgaaa caaaaaacct gacagttgaa tgggaagaag ttccagcgga aaatctgata
     8041 aaagcaggtc aatttaccgt tcgaggccat gtccttggta gtgatcttgt tgctgaggtc
     8101 actgtacgag tgacagacaa actaggcgaa gctctttcag ataaccctaa ctatgatgaa
     8161 aacagtaacc aggcctttgc ttcagcaacc aatgatattg acaaaaactc tcatgaccgc
     8221 gttgactatc tcaatgacgg agatcattca gaaaatcgtc gttggacaaa ctggtctcca
     8281 acaccatctt ctaatccaga agtatcagcg ggtgtgatct tccgtgaaaa tggtaagatt
     8341 gtagaacgga ctgttgctca agccaaactt cacttctttg cagatagtgg tacggatgca
     8401 ccaactaaac tcgttttaga acgctatgtc ggtccagagt ttgaagtgcc aacctactat
     8461 tcaaactacc aagcctacga cgcagaccat ccattcaaca atccagaaaa ttgggaagct
     8521 gtgccttatc gtgcggataa agacatcgca gctggtgatg aaatcaacgt aacatttaaa
     8581 gctgtcaaag ccaaagctat gagatggcgt atggagcgta aagcagataa gagcggtgtt
     8641 gcgatgattg agatgacctt ccttgcacca agtgaattgc ctcaagaaag cacgcaatcg
     8701 aagattcttg tagatggaaa agaacttgct gatttcgctg aaaatcgtca agactatcaa
     8761 attacctata aaggtcaacg gccaaaagtc tcagttgaag aaaacaatca agtagcttca
     8821 actgtggtag atagtggaga agatagcctt ccagtacttg ttcgcctcgt ttcagaaagt
     8881 ggaaaacaag tcaaggaata ccgtatccag ttgactaagg aaaaaccagt ttctgagaag
     8941 acagttgctg ctgtacaaga agatcttcca aaactcgaat ttgttgaaaa agatttggcc
     9001 tacaagacag ttgagaaaaa agattcaaca ctgtatctag gtgaaactcg tgtagaacaa
     9061 gaaggaaaaa ctggtaaaga acgtatcttt acagcgatta atcctgatgg aagtaaggaa
     9121 gaaaaactcc gtgaagtggt agaagctccg acagaccgca tcgtcttggt tggaaccaaa
     9181 ccagtagctc aagaagctaa aaaaccacaa gtgtcagaaa aagcagatac aaaaccaatt
     9241 gattcaagtg aagctagtca aactaataaa gcccagttac caaatacagg tagtgcggca
     9301 agccaagcag cagtagcagc aggtttagct cttctaggtt tgagtgcagg attagtagtt
     9361 actaaaggta aaaaagaaga ctagaaattt tgataccttc tttatcatta aagaccaaag
     9421 aaaaactctt gtcctaaata gaagataaag agtctggaac aaaatagttc tcaaccacaa
     9481 aaaagctaga tatttccaat tgcggaactc tagcttttta attttgagtc gttctcttat
     9541 tgtatagtag attgaaacta gagtagtaca cttctacttc taaaacattg ttagaaatcg
     9601 atttgactgt ccttatcgat ttgtcctgtt cttatttcat ttcactatgt tttaggaggt
     9661 tattggccat aagtaccaat cccatgtcaa ttctcacttg acgcttgcct ctcagattac
     9721 atctcttgta acccaaacaa gcctttatct gcccaaagac aggttctaca tcaatcttgc
     9781 gttgagcgaa aatctgtcta tcttggggag ataaaagcgc ctgacattct ttagctttca
     9841 agttttgata gcgttcgttc atatacaatc ccttttgagg ggctgattca ggttcatcag
     9901 cgtagtaaac cttgatttcc tgttgaaagt ctgtctgtgt tttctggtac ttgatatggt
     9961 gaaaacgata gcaccagcca tcaggatgta tgtagctatc ctccttgtca ttatagtgcc
    10021 aattcgctaa gtttttagct gactgtttat accctctctt ttgttcctta tcaaacatgg
    10081 catatttaat cagaagggga gtgatttctt cttgaaaata gacttgtatc tgttcttgaa
    10141 gtttggcgga gaatttctct gtcgttttct tccacacaaa actatacttg ttggcattgg
    10201 attcaatctt agttccgtca ataaacaaac aatctaaggt cactaactct tccatttttc
    10261 aatttttcga ccagagaaaa tcccttgtga ataggcaaat agaagagtag atacaagcat
    10321 tttagggtga taagacgggc gaccaaaggc atgataggag gtgtagaagt gacgttcctc
    10381 caaggtattc accacttttt caatagtaaa gacgagatgg tcttgtggta agaaggaact
    10441 gatttctagt ggtaaagttg tttgatttgt gttatagtga atatgcatgg gatagccttt
    10501 ctaaatggtt tattaagcaa ttctatttta ccaagactat cgtaaccatg caaaaaagca
    10561 acggcaaatc cgttgccttt tttggatata agagactatt gtcccagact cttttctttg
    10621 tataaaattg tatagtgtta ttgtctatac tattttgtag agaaagcaaa aatccctaga
    10681 aaatgcttag ggattcgact atttcattaa gaacaattca acaatcatct ctatatacat
    10741 gataagggtt aataaaaatc ctgtacgcca gaagaatttg atgaatttag gatagtaaaa
    10801 gctgcgtttt tttaaaagga agaaaaagac gagaataatg gctaggaacg agagggctag
    10861 ccccagtcta gggaggaaat tatgggtaaa ggttttagct gtaatcaagt aatactcaaa
    10921 taccaagact ggaaaagcca aatccgcaaa gtttcgtcct agttttttta atctaaaaag
    10981 tttggttagg ataatgcaga cgactaaggt cagtatcaat aataaaatag atgctaattt
    11041 cattaaaatc atacccatat tgtatcataa aaaaaggcta atggaaacga gaaacagagg
    11101 aatttatagg aaagtcaggc ttttgagatt gtgggtgatt tttgttataa tgaaagttat
    11161 aaaatcttat gataccttga atgattatta tcgaaaactc tttggagaaa agacttttaa
    11221 agtccctatt gatgcgggat ttgactgtcc caatcgtgat ggaactgtgg ctcatggagg
    11281 ctgtactttt tgtacggttt ccggttctgg agatgccatt gtagccccag atccgcctat
    11341 ccgtgagcaa ttttataagg aaattgactt tattcatcgt aagtggccag atgttcagaa
    11401 gtatctggtt tattttcaaa attttaccaa cacccatgaa aaggtggaag ttattcgaga
    11461 gcgttatgaa caggctatca atgagccagg tgtggtagga atcaatattg gaacgcgccc
    11521 agactgttta ccagacgaaa ccatcgaata tttggctgag ttatcggagt gcatgcatgt
    11581 gacggttgaa ttgggcttgc agactactta tgaagcaacc tctgacctga ttaaccgtgc
    11641 gcattcttat gaattgtagg tggaaacggt caagcgtttg agaaagtatc caaaaattga
    11701 gattgtttcc catttgatca atggtttgcc tggtgaaacc catgagatga tggttgaaaa
    11761 tgtccgccgt tgtgtcacgg ataacgatat tcaagggatt aaactgcatt tgctccatct
    11821 tatgacaaat actcgtatgc aacgagatta ccatgaggga cgtttacaat tgatgagtca
    11881 ggacgaatat gtcagggtca tctgtgacca actggaaatt attcccaaac atatcgtcat
    11941 ccatcgaatt acaggagatg cgcctaggga tatgctgatt ggtcctatgt ggagcctcaa
    12001 taaatgggaa gtcctcaaca gcattgagat ggagatgaga cgtcgtggaa gtgttcaagg
    12061 atgcaaggct gtaaaacagg agtttgaaaa tgaaaagacc acttgagatg gcacatgatt
    12121 ttttggctga ggtcgtgaca aaagaggatg tcgtagtgga tgcgactatg ggaaatggtc
    12181 atgacacgct ttttttagcc aagctagcca agcaagtcta tgcctttgat attcagaagc
    12241 aagccttgga aaagacccaa gagcgtttgc atcaggctgg cttgacaaat gcccagttaa
    12301 tcttgcaagg ccatgagaca ctggaccagt ttgtgacaaa agctaaggca gggattttta
    12361 atttgggcta tttgccgtca gctgataagt ctgtcatcac ccgaccgcag acaacgattg
    12421 aggcattaga aaagctatgt ggcttacttg tcaaaggtgg acgaattgct attatgattt
    12481 actatggtca tgaaggaggc gacctcgaga gagatgctgt cttggatttt gtgatccagt
    12541 tgaaccaaca agagtacaca gctgccattt accgaacttt aaaccaagtc aacaacccgc
    12601 cgtttttagt gatgattgaa aaattagaga gatacagaca tggataaaca atacctacac
    12661 gaaaagctgg atgccatgcg ccagaatttt gttgaatcaa cctaccacga acgagcggtg
    12721 ggcgtgctag accaagcgca tatgagcaaa aaaatgctta aaatcaagaa aaaattagtt
    12781 gctcttgaaa tggaacggtg ccagagaaaa attgagcaca aagactgttc caagattgac
    12841 caaaaaatca aagagcagaa ggagatattt gaatcctgtt gtaaaaaaga ttaaggagga
    12901 cgtgcgtgga attactgatt tacctcatcc tatttttact ggtcttgatt gtctcaagta
    12961 caaccaataa gctcctaccc tttttgcctc tccctttggt gcagattctt ttgggaattg
    13021 tgattggtct ctttttaccc aatactgact ttcaccttaa tacggagttg tttttggcac
    13081 tggttatcgg ccccttgctt ttccgagagg ctgaagaagc agatgttacg gctattttaa
    13141 aacactggcg aatcattgtt tatctcatat ttccagtgat ttttatctcg accctgagtt
    13201 tgggtggctt ggcccatctt ctttggttca gccttccctt ggcagcttgc ttggctgttg
    13261 gggcagccct tggtcctacg gacttggtgg cctttgcctc tctttcggag cgttttagct
    13321 ttcctaagcg cgtgtccaat attcttaagg gcgaaggact cttgaatgat gcttctggtt
    13381 tggtggcttt tcaggtagct ttgacagctt ggacaactgg agctttttct ctggggcaag
    13441 ctagcagttc actcatcttt tcaatcctag gcggtttttt aattggattt ttaacagcca
    13501 tgaccaaccg cttcctccat accttcttgc taagtgtgcg tgcaacggat attgccagtg
    13561 aacttttatt agaattgagt ttgcctcttg tgaccttctt tctggcagaa gaagttcatg
    13621 tttcaggtat tattgccgtc gtagttgctg gaattttaaa ggcaagtcgt ttcaagaaaa
    13681 tcacgctcct cgaagcccaa gtggatacgg tgaccgagac ggtctggcat acagtgacct
    13741 ttatgctcaa cggttctgtc tttgtgattt tagggatgga gctggaaatg atagcagaac
    13801 ctatcttgac caatccaatc tataatcctc tacttttatt gctatctctt atcgccctta
    13861 cctttgtcct ctttgtcatt cgttttatta tgatctatgg ctattatgcc tatagaaccc
    13921 gacgcctcaa gaaaaagcta aataagtata tgaaggacat gtttctcttg accttttcag
    13981 gtgttaaggg aacggtgtcg attgctacga ttctcttgat accaagtaat ctagaacagg
    14041 agtatcctct cttgcttttc cttgttgcag gtgtgacgct tgttagcttt ttaacaggtc
    14101 tcttggtctt gcctcatctt tctgatgaag aggaagaaag caaggattat ctcatgcata
    14161 tcgccatttt gaatgaagta acgctagagt tggaaaaaga gttggaagac accagaaata
    14221 aactttccct ctatgcggct attgacaatt atcatggacg tattgaaaat ctcattttaa
    14281 gccaagaaaa ccaggatgat caagaagact gggctgcttt gaaactcttg attcttagta
    14341 ttgaaagtga tggtttggaa caggcctatg aagaggggaa tattagcaat cgtgcttacc
    14401 gagtttacca acgttatctg aaaaatatag aacaaggaat caatcgtaaa cttgcctcaa
    14461 gactgaccta ttattttctt gtttccttga ggattttacg ttttctcctt catgaagttt
    14521 ttactcttgg aaagaccttc cgtagctgga aggacaagga gcaaagccgt ctccgtgctc
    14581 ttgattatga ccaaattgca gagctctatc ttgccaatac agagatgatt attgaaagtt
    14641 tggaaaacct gaagggagtc tacagacgct ctttgattag ttttatgcag gagtctcgtc
    14701 ttcgagaaac agctattatc agcagtggtg cctttgtcga acgggttatc aatcgtgtca
    14761 aacccaacaa tatcgatgaa atgctgagag gctattatct ggagcgcaag ttgattttcg
    14821 aatacgaaga aaaacgatta attacaacta agtatgccaa gaaattacga caaaatgtaa
    14881 ataacttaga gaactattcc ttgaaggaag ctgccaatac cctgccgtat gatatggtgg
    14941 aattggtaag aagaaattag ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc
    15001 gccttggatt atatatgtga ctgacttcgt cagtttcatc tacaacctca aagcagggct
    15061 ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tataagatta
    15121 taaatgaagg agtatgacat gaaaaagtgg tggaaagagc tgatagacaa gcctttatta
    15181 aaagcttttt tgcattatta tcaagcatca gatagtgagt tgaccagtgt cgcagtagcc
    15241 tactattggt tgatttcgat tttccccctg cttttggtgg tggtcaatat cctcccctat
    15301 tttcagattc ctgtgggaga atttctgtgc tttatgaaag acgttctgcc cccaagcctc
    15361 tatgaaggtg tggaaaaaat agccagagaa gttttgacgc aaccttcaac aggtttattg
    15421 agtttttctg ttttatcggc gttatggagt ttttcaaaat ctatgaattt cttgcaaaaa
    15481 gcttttaaca aggcttatgg cgtcgaaaag agtcgtggac ttatttccca tcagatgcta
    15541 agtctcctag tcagttttgg cttgcagctc ctctttgcgt ttgccttgtt tttgatctta
    15601 tttggtcaga tgattttggc tttacttgct cattattgga caaaagatgg cattatctat
    15661 caggctttac aagggttggc aggtcctctg atttacgccc tcctctttgc tatcttggtc
    15721 atgctttatt attttcttcc caatatatct aacaggaaaa ttagctatac cctaccaggc
    15781 agtgcctttg ttctcttagt gattttaggt ttactaactc tgttctctag ttatctcaat
    15841 tactatgtcc atcatttggt ggatgtccga attttaggtt ccgttcttct tgttgttatg
    15901 atgttttggt ttatcttgat tgctaaaatt gttatcctag gcgcggttat caatgccagt
    15961 atgcagagtt tgagagatcc ggtctttaaa aaagactaac tatttatcca ttacaaaagc
    16021 gcatagtatc aaaactttat agaaccttga tactatgcgc ttttgtgctt tgacagtttt
    16081 ttgtatagtt tttgtccaag atttctaaat catctgatta aattcctatt ttcccctatc
    16141 attgtccctg ttttttcgaa aatggaagga gtataataaa tctatcaata aaatgatagg
    16201 aggaacttta tgggtcatat tttctttttt ctaagtgtct ttttggcagg gattctatcc
    16261 ttcttttctc cttgtatctt acctttgtta ccggtctata caggagtgtt actagatgat
    16321 aaggatggtg ctcaggcttc tagcggcaaa ttttcaatct cagttactag tttattacga
    16381 actctggcct ttatagcagg aatttccttt atatttattt tgttgggcta tggagctggt
    16441 tttttaggcg atttgcttta tgcttcttgg ttccaatatc ttactggggc aattattatc
    16501 cttcttggtt tgcaccaaat ggagattcta cactttaagg ggctttataa ggaaaagagg
    16561 ctacaactgc aaggacaggg gcaaaatggt aagggctata gtcaggcatt tttattgggc
    16621 ttgaccttta gttttgcttg gacgccttgc gtggggccgg ttctggggtc tgttttggcc
    16681 ttggcggctt caggtggttc aggagcttgg cagggagctg gtctcatgtt ggtgtatacg
    16741 ctgggcttgg cgctaccatt cttgcttcta gctctgacct ctagttatat tttgaaacat
    16801 ttccgaaaac ttcatcccta tctcggaatc ctcaaaaaag tgggtggttt tctcattatt
    16861 gtgatgggac tcttggttct gtttggaaat gcttcaattt taagtcaatt atttgaataa
    16921 aatggaaagg aatatcaata tgaaaaaatg gcaaacatgt gttcttggag caggttcgct
    16981 cctttgtttg acggcttgtt caggcaagtc cgtgactagt gaacaccaaa cgaaagatga
    17041 aatgaagacg gagcagacaa ctagtaaaac aagcgcagct aaagggaaag aggtgactga
    17101 ttttgaattg atgggagtag atggcaagac ctaccgttta tctgattaca agggcaagaa
    17161 agtctatctc aaattctggg cttcttggtg ttccatctgt ctggctagtc ttccagatac
    17221 ggatgagatt gctaaagaag ctggtgatga ctatgtggtc ttgacagtag tgtcaccagg
    17281 acataaggga gagcaatctg aagcggactt taagaattgg tataagggat tggattataa
    17341 aaatctccca gtcctagttg acccatcagg caaacttttg gaaacttatg gtgtccgttc
    17401 ttacccaacc caagccttta tagacaaaga aggcaagctg gtcaaaacac atccaggatt
    17461 catggaaaaa gatgcaattt tgcaaacttt gaaggaatta gcctaggagg cgtcttatga
    17521 atgataagtt aaaaatcttc ttgttgctag gagtattttt tctagccata accggtttct
    17581 atgttctatt gatacgaaat gcagggcaga cagatgcctc gcaaattgaa aaggcggcag
    17641 ttagccaagg aggaaaagca gtgaaaaaaa cagaaattag taaagacgca gacttgcacg
    17701 aaatttatct agctggaggt tgtttctggg gagtggagga atatttctca cgtgttcccg
    17761 gggtgacgga tgccgtttca ggctatgcaa atggtagagg agaaacaacc aagtacgaat
    17821 tgattaacca aacaggtcat gcagaaaccg tccatgtcac ctatgatgcc aagcaaattt
    17881 ctctcaagga aatcctgctt cactatttcc gcattatcaa tccaaccagc aaaaataaac
    17941 aaggaaatga tgtggggacc cagtaccgta ctggtgttta ttacacagat gacaaggatt
    18001 tggaagtgat taaccaagtc tttgatgagg tggctaagaa atacgatcaa cctctagcag
    18061 ttgaaaagga aaacttgaag aattttgtgg tggctgagga ttaccatcaa gactatctca
    18121 agaaaaatcc aaatggctac tgccatatca atgttaatca ggcggcctat cctgtcattg
    18181 atgccagcaa atatccaaaa ccaagtgatg aggaattgaa aaagaccctg tcacctgagg
    18241 agtatgcagt tacccaggaa aatcaaacag aacgagcttt ctcaaaccgt tactgggata
    18301 aatttgaatc cggtatctat gtggatatag caactgggga acctctcttt tcatcaaaag
    18361 acaaatttga gtctggttgt ggctggccta gttttaccca acccatcagt ccagatgttg
    18421 tcacctacaa ggaagataag tcctacaata tgacgcgtat ggaagtgcgg agccgagtag
    18481 gagattctca ccttggccat gtctttacgg atggtccaca ggacaagggc ggcttacgtt
    18541 actgtatcaa tagcctctct atccgcttta ttcccaaaga ccaaatggaa gaaaaaggct
    18601 acgcttattt actagattat gttgattaat actcttcgaa aatctcttca aaccacgtca
    18661 gcgtcgcctt accgtatgta gggttactga cttcgtcagt tttatctaca acctcaaagc
    18721 ggtactttga gaaacctgtg gctatcttcc tagtttactt tttgattttt attgagtata
    18781 agaggtcttt cctaagcagt tagaggagaa ttttgctata ctgatactag taagtggtaa
    18841 aggagcagat catgacctac acaatcttaa tcgtagaaga tgaatatctg gtaagacaag
    18901 gtttgactaa actggtcaat gtagcagcct acgatatgga aatcatcggt caggctgaaa
    18961 atggaaggca ggcttgggaa ttgatccaaa agcaggtgcc agatatcatt ttaaccgata
    19021 tcaacatgcc tcatctaaat ggcatccagt tggccagtct ggtacgagaa acctatcctc
    19081 aggttcattt ggtcttttta acaggttacg atgattttga ttatgccttg tctgctgtca
    19141 aactaggtgt ggacgactac ctgctcaaac ccttttctcg tcaggatatt gaggaaatgt
    19201 tggggaaaat caaacaaaaa ctagacaagg aagagaaaga agagcagtta caagatttat
    19261 tgactaatag gtttgaagga aacatggccc agaaaatcca gtctcatctg gctgatagcc
    19321 aatttagttt aaagtcttta gccagtgact taggttttag tccgacctat ctgagttcct
    19381 tgattaagaa agagttgggc ttgccttttc aggattatct ggtgagagaa cgtgttaaac
    19441 aagccaagct cttgctttta actacagatc tgaagattta tgagatcgca gagaaggttg
    19501 gttttgaaga tatgaactat tttacccaac gttttaagca gattgcaggt gtgacacctc
    19561 gtcagtttaa gaagggagaa gaccgatgaa gcgttcttct cttttagtta gaatggttat
    19621 ttccatcttt ctggtctttc tcattctcct agctctggtt ggaactttct actatcaatc
    19681 aagttcttca gccattgagg ccaccattga gggcaacagc caaacgacca tcagccagac
    19741 tagccacttt attcagtctt atatcaaaaa actagaaacc acctcgactg gtttgaccca
    19801 gcagacggat gttctggcct atgctgagaa tcccagtcaa gacaaggtcg agggaatccg
    19861 agatttgttt ttgaccatct tgaagtcaga taaggacttg aaaactgttg tgctggtgac
    19921 caaatctggt caggtcattt ctacagatga cagtgtgcag atgaaaactt cctctgatat
    19981 gatggctgag gattggtacc aaaaggctat tcatcaggga gctatgcctg ttttgactcc
    20041 agctcgtaaa tcagatagtc agtgggtcat ttctgtcact caagaacttg ttgatgcaaa
    20101 gggagccaat cttggtgtgc ttcgtttgga tatttcttat gaaactctgg aagcctatct
    20161 caatcaactc cagttggggc agcagggctt tgccttcatt atcaatgaaa accatgaatt
    20221 tgtctaccat cctcaacaca cagtttatag ttcgtctagc aaaatggagg ctatgaaacc
    20281 ctacatcgag acaggtcagg gttatactcc tggtcacaaa tcctacgtca gtcaagagaa
    20341 gattgcagga actgattgga cggtacttgg cgtgtcatca ttggaaaagt tagaccaggt
    20401 tcggagtcag ctcttgtgga ccttgcttgg ggccagtgtc acatctcttc ttgtctgtct
    20461 ctgcttagtg tggttcagtc ttaaacgctg gattgctcct ttgaaggatt tgagagaaac
    20521 catgttggaa attgcttctg gtgctcaaaa tcttcgtgcc aaggaagttg gtgcctatga
    20581 actgagagaa gtaactcgcc aatttaatgc catgttggat cagattgatc agttgatggt
    20641 agctattcgt agccaggaag aaacgacccg tcagtaccaa cttcaagccc tttcgagcca
    20701 gattaatcca catttcctct ataacacttt ggacaccatc atctggatgg ctgaatttca
    20761 tgatagtcag cgagtggtgc aggtgaccaa gtccttggca acctatttcc gcttggcgct
    20821 caatcaaggt aaggacttga tttgtctctc tgacgaaatc aatcatgtcc gccagtatct
    20881 ctttatccag aaacaacgct atggagataa actggaatac gaaattaatg aaaatgttgc
    20941 ctttgataat ttagtcttac ccaagctggt cctacaaccc cttgtagaaa atgctcttta
    21001 ccatggcatt aaggaaaagg aaggtcaggg ccatattaaa ctttctgtcc agaaacagga
    21061 ttcgggattg gtcatccgta ttgaggatga tggcgttggc ttccaagatg ctggtgatag
    21121 tagtcaaagt caactcaaac gtgggggagt tggtcttcaa aatgtcgatc aacggctcaa
    21181 acttcatttt ggagccaatt accagatgaa gattgattct agaccccaaa aaggaacgaa
    21241 agttgaaata tatataaata gaatagaaac tagctaactc ccagtcaatt ctgggagttt
    21301 tatgcgtaat tgggcaaact ttctaggttg tctatcttgc taaaaacaag aaaaaacggt
    21361 atgatagaag agtgacaaca gaggtaacag agatgaacga gacacagatt caaagagaaa
    21421 cacgtcaggt agtggaagat gttttagaaa agaccaattt gaaacagggg gccctttttg
    21481 ttttaggtct atcttctagt gaggtgctag gtggtcagat tgggaaagaa tccagtcaag
    21541 agattggtga gctgatagtg gagactatct tgggtattct aggtagtaga ggtatccatt
    21601 tagcagttca aggttgtgag catgtcaatc gggctctcgt tgttgaacgt caggtggcag
    21661 agcagtttgg tctggaaatt gtcagtgtcc atcctactct tcatgcagga ggttcggggc
    21721 agttggcagc ctttaagttt atgcaggatc cagttgaggt tgaatttatc aaggctcatg
    21781 ctggattgga tatcggagac actgcaattg gcatgcatgt caagcatgtt caggttccga
    21841 ttcgccctat tttgagagag attggtcatg cccatgtaac ggcactgact agtcgtccaa
    21901 aattaatcgg aggtgcgcgt gcgcactatc cgcaagatgc tattaggaag ttttgagaat
    21961 gaataaagga gataaaagtg tttaaaaaag accgtttttc aattcgtaag attaagggag
    22021 ttgtaggctc tgtatttctt ggaagccttt tgatggctcc ttctgtagtg gacgcagcca
    22081 cctatcacta tgtaaataaa gagattattt cacaagaagc taaagattta attcagacag
    22141 gaaagcctga caggaatgaa gttgtatatg gtttggtgta tcaaaaagat cagttgcctc
    22201 aaacagggac agaagcatct gttttgacag cttttggttt gctgactgtt gggagcttgc
    22261 ttttaatcta caagagaaag aaaattgcta gcgtctttct agttggagct atgggattgg
    22321 tagttcttcc tagtgcagag gctgtagacc cagttgcgac cctagcgctg gctagtcgag
    22381 agggtgttgt tgaaatggat ggctatcgct atgttggtta tctatcaggt gacatcctca
    22441 aaacgcttgg cttggacact gttttagaag aaacctcagc aaagcctgga gaggtgactg
    22501 tagtcgaagt tgagactcct caatcaacaa caaatcagga gcaagctagg acagaaaacc
    22561 aagtagtaga gacagaggaa gctccaaaag aagaagcacc taaaacagaa gaaagtccaa
    22621 aggaagaacc aaaatcggag gtaaaaccta ctgacgacac ccttcctaaa gtagaagagg
    22681 ggaaagaaga ttcagcagaa ccagctccag ttgaagaagt aggtggagaa gttgagtcaa
    22741 aatcagagga aaaagtagca gttaagccag aaagtcaacc atcagacaaa ccagctgagg
    22801 aatcaaaagt tgaaccacca gtagaacaag caaaagtccc agaacaaccc gtgcaaccta
    22861 cacaagctga gcaaccaagt acaccaaaag aatcatcaca acaagaaaat cctaaagaag
    22921 ataggggagc ggaagagaca ccgaaacaag aagatgaaca gccagcagaa gccccagaaa
    22981 tcaaggttga agaaccagta gaatcaaaag aggagactaa aacagctaaa ggtacgcaag
    23041 aaaaaggtca cgtgggtgag gctcttgctc aagaacaatt acctgagtac aaagtaactg
    23101 agggaaccct tgttgaaaca tcgactacag acctagacta caaaactgaa acgactgagg
    23161 atccaaccaa gtatacggac gaagaaactg tcctacgaac tggtgaaaaa ggaagtcaag
    23221 ttacaaaaac aacctataaa acagtagaag gtgttaaaac tgaccaagtt ctttcaacca
    23281 gtacggaagt gaccaaggag cctgtaaatc aacaggtaat ccgtggtaca aaaccaattg
    23341 aaggaactct tatagaagaa agtcttgaaa agattccatt taaggaagtt gttaaagaag
    23401 atgaccaact gaaaaaaggc ttagaagttg tagctcaaga aggaaaagaa ggccagaaaa
    23461 aaatcaccaa gacctttaat accattaagg gagttaaaac agaagacgct ccaaaagtta
    23521 cagaggaaat ccttgaggaa cctcaagaca aaattttgaa acgtggtacc aagagctttg
    23581 aaaaaccagt gttgaccatc acagaaattg aatctaagga tttgaagcgt acttcagatg
    23641 ttaagtatag tttagaaaat ccaagtaagg cagccattaa atctattacc ctaactctga
    23701 aaaaaggtga tgagattgtc aaaaccttga atgtttcacc agaagacttg acaactaccc
    23761 tgacagattt gcagtactac aaggattata aacttgaaac aaagatggtt tatgaccgtg
    23821 gtgaggggga tgaggaagaa gttctgaaag aagaaccgct aagaattgat ctcaaaaaag
    23881 ttgaaataaa agatattata agaacggatt taatcaaata tgaaaaccaa gttgaaactg
    23941 atgaaactcg attgacttca gtgccaactg atttgtcaaa ttattattta aaagttacct
    24001 ctaatgacca taagaccaat ctactagcgg ttcaaaaaat tgaagagtca acaattgatg
    24061 gaaaacaagt ctacaaggta atagcagtag cggataactt ggtccaacgt aatgtcgata
    24121 atcgctttga agaaacttat acctactata ttgaaaaacc caaagctagt caggctggta
    24181 ttttctacga ctttgaagaa ttgatccaag ccattcagac aaatcctgcc ggtgaattta
    24241 aactgggtca aagcatgagc gctcgtaacg ttgttcctaa agggaaatct tatattactt
    24301 cagagtttac tggtaaacta ttgagtgatg gtgataaacg ctatgctatc cataatttgg
    24361 aacatccttt attcgataag attaaggctg gaactgttaa aaatataaat tttgaaaata
    24421 ttgatattga tttatctaat caagataata tcgctgcaat agcaaatgaa atcagtggaa
    24481 cgacacgtat agaaaatatc aaagcaatcg gaaccataac agctagaaat aatgttgctg
    24541 ggttcgtaaa taacataaaa gaagacgcta ctttagaaaa tgtggcattt atcggtagta
    24601 taaattctgc tggtaataat tcaacagttg gaggtattgc cggttctaac tatatggggt
    24661 ttgttaatag agcctacgtt gatgctacta ttacagcaaa taacgcaaat gcaagtatgc
    24721 ttgttccttt cgttcaatat aaagctcata gctggctatc agggacaaaa ggaaaactta
    24781 caaactctgt agcaaaaggt attcttgatg ttaaaaatac taaatatgtt ggtggaataa
    24841 cctctaaaac ttggccatat ggagcggttc aagataatgt aacatatgcg aaagttatta
    24901 aaggtcagga aatattcggt tcaaacgatg ttaatgatgg agatgcaaat ccttttgtgt
    24961 ctaatttatt tggagttatt ggatatagct cttctgaaga tggaactggt aaagataata
    25021 aaaacaaaaa taaattaaaa cacttatcta aatctgaggc tgatgcaaaa gttgctagtt
    25081 tcaatattac tgctgctaaa ttcgtaagtg aaccttatga attgaacaaa ttgaataatg
    25141 ttagcagtcg tgaagcagct tacgagtcta ttcaagatta ta
//
LOCUS       DAVLFB010000021        24778 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLFB010000021 DAVLFB010000000
VERSION     DAVLFB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.021"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(515..1363)
                     /locus_tag="VXD58_000758"
     CDS             complement(515..1363)
                     /locus_tag="VXD58_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV0675498.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1515..1793
                     /locus_tag="VXD58_000759"
     CDS             1515..1793
                     /locus_tag="VXD58_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675499.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1795..3858
                     /locus_tag="VXD58_000760"
     CDS             1795..3858
                     /locus_tag="VXD58_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0675500.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLVRTKELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3936..5192)
                     /locus_tag="VXD58_000761"
     CDS             complement(3936..5192)
                     /locus_tag="VXD58_000761"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV0675501.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5334..7799
                     /locus_tag="VXD58_000762"
     CDS             5334..7799
                     /locus_tag="VXD58_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0675502.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7864..8775
                     /locus_tag="VXD58_000763"
     CDS             7864..8775
                     /locus_tag="VXD58_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV0675503.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            9005..9703
                     /gene="dapD"
                     /locus_tag="VXD58_000764"
     CDS             9005..9703
                     /gene="dapD"
                     /locus_tag="VXD58_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV0675504.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9771..10901
                     /locus_tag="VXD58_000765"
     CDS             9771..10901
                     /locus_tag="VXD58_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV0675505.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10913..11452
                     /locus_tag="VXD58_000766"
     CDS             10913..11452
                     /locus_tag="VXD58_000766"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV0675506.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11436..12113
                     /locus_tag="VXD58_000767"
     CDS             11436..12113
                     /locus_tag="VXD58_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV0675507.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12185..13084)
                     /gene="galU"
                     /locus_tag="VXD58_000768"
     CDS             complement(12185..13084)
                     /gene="galU"
                     /locus_tag="VXD58_000768"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV0675508.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13106..14122)
                     /locus_tag="VXD58_000769"
     CDS             complement(13106..14122)
                     /locus_tag="VXD58_000769"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0675509.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14334..14774
                     /locus_tag="VXD58_000770"
     CDS             14334..14774
                     /locus_tag="VXD58_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0675510.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(14897..15313)
                     /locus_tag="VXD58_000771"
     CDS             complement(14897..15313)
                     /locus_tag="VXD58_000771"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0675511.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(15381..16031)
                     /gene="phoU"
                     /locus_tag="VXD58_000772"
     CDS             complement(15381..16031)
                     /gene="phoU"
                     /locus_tag="VXD58_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV0675512.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16046..16798)
                     /gene="pstB"
                     /locus_tag="VXD58_000773"
     CDS             complement(16046..16798)
                     /gene="pstB"
                     /locus_tag="VXD58_000773"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV0675513.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16800..17615)
                     /gene="pstA"
                     /locus_tag="VXD58_000774"
     CDS             complement(16800..17615)
                     /gene="pstA"
                     /locus_tag="VXD58_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV0675514.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17608..18471)
                     /gene="pstC"
                     /locus_tag="VXD58_000775"
     CDS             complement(17608..18471)
                     /gene="pstC"
                     /locus_tag="VXD58_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV0675515.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18589..19464)
                     /locus_tag="VXD58_000776"
     CDS             complement(18589..19464)
                     /locus_tag="VXD58_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV0675516.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19631..20962)
                     /locus_tag="VXD58_000777"
     CDS             complement(19631..20962)
                     /locus_tag="VXD58_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV0675517.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20963..21670)
                     /locus_tag="VXD58_000778"
     CDS             complement(20963..21670)
                     /locus_tag="VXD58_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675518.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21754..22230)
                     /locus_tag="VXD58_000779"
     CDS             complement(21754..22230)
                     /locus_tag="VXD58_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV0675519.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            22237..22764
                     /locus_tag="VXD58_000780"
                     /pseudo
     CDS             22237..22764
                     /locus_tag="VXD58_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23079..24770)
                     /locus_tag="VXD58_000781"
     CDS             complement(23079..24770)
                     /locus_tag="VXD58_000781"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV0675520.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
BASE COUNT         7152 a         4738 c         5358 g         7530 t
ORIGIN      
        1 ggacggattt attttggatt ttatttgtta ttttgacctg cctctatatt ggtaaccatg
       61 atttgtttac tctcaatcat caagaattct cttttcgtgg tagcgtttgg ggtctggtac
      121 tggccttata tcacttattg ttcattgata agtttgtaat ttcgaatcga aaataaagat
      181 tagaactatg attgactggc tacttttaat attgattttg gaggaagatt ttgtctctat
      241 tatttattat tttaaattta tctattttgt acaagattta ttctttgaga cgggagaaat
      301 cgaaatactt gatttatacg gcctatatca tatttggggt aaatgtagta tatggtattc
      361 aatggttatt aagagaactg atttcaacta tttcccctta atgtgtatgg aaactataac
      421 aaaacgcata agattaaggt ttgtgtacct gttcttatgc gtttttataa tattgaagat
      481 tttcggtaag ttttatacct ttgggaagtc tagtttagaa tgctttccca accaaaatct
      541 ctgcttcgat ggtaatctct gtcagttggc tccagtcaat cttgctctgg tcaacgtgaa
      601 agaggagagg agtcatactg agtagggctt ggagttgttc tgctgtgata gtcttagtca
      661 gagaggcagt ttgactagat aggatggtaa agtgttcctg gaaatgctcc ttgatatctt
      721 ggttagaata ctccttgttt gtcagctggt cctgtactct ttgacggatt tctttgaggt
      781 gattttcagt tgggataacc tttatcaaga taccgtcttt ggataaaacg cgacgaaatt
      841 ctccatagtt ggcaggtgag aagatatcaa gcagaatatc catgttagcg tcttttatag
      901 gaagtcgtgc caagtcgcca acaaaccaat tgactgccca gttgggttca cttttagcag
      961 cgatttggac tgaatctttg gagatgtcaa aggcatagaa agttttttca gagtgacttt
     1021 cttgtagttt gcgagaatag aatccttcac cacaaccgat atccaaaatt gttgtggtag
     1081 tttttgagct tgcaagcaag tcagatacag catctaagat agcttggtaa aagccggctt
     1141 ctaggatttg ttgacggttt tgaaaatttt ccttgttata gttagcagat tgcttgattt
     1201 gaggtgctag attgacatag ccaaatttcg ccaagtcaaa agaatgacgg ttgcagcact
     1261 tgaaattagt ctctaacaga gtcagatttt cttgacagat aggacaggca aaggcagtcg
     1321 cagaagcaaa acgctgaagt ttgggcttga gatttgtatt catagtttaa gtgtatcaaa
     1381 agaggcaaat gaaagcaact ttaggaataa gtagatggga gattcagtag aaataaaact
     1441 aattctccaa tttatagaat gaaattgctt ttatatctaa attgctatat aataatgata
     1501 aggaggaaat aagtatgtta aaagaagtat taaccgttgc aaaagttgcg aaaaaatctt
     1561 cattattttt gggtggtgtc gcatttggta cccttggttt gaaaatctta gcaagtaagg
     1621 aagctaaaaa aggttattct aaagctttgg ctaaggctta caagttgaaa gacgagctag
     1681 atgcatctgt ttctgttgtg aagcaacatg gagacgatgt cttgcaagat gccaaatatt
     1741 tgtacgagca agagaaaaaa gaagagcaat tagatagcct tataggtgaa taatatgtct
     1801 tttaaagtgc tacatagagg ataccaacat atccgactat catcttcttt ttcacttacc
     1861 ttggatattc aagactatct tcgttccttg gcgagagatg aaaaggggat tgagtctatc
     1921 cagttttaca tggatcaaca gcactttact ctacgcataa aagaaggctt ttctgtatta
     1981 gataatgcag aagccttttt aaaaagaatt gataaaggga aagtttctga gttgatgact
     2041 cttcccattc gtagagaaga gagtgcttat tctattgttt caggtgcagc ggttaagcgt
     2101 gtactttttc gtagttttgt gccgtatcct attcgctata tatggacttg ttatcaggct
     2161 ttgggttata ttagagaagc ctatcaaaca ctagcgcgta aggaactaac gatggaagtc
     2221 ttggactgtt cggctatttt attgtctttg tttatgaacc aatccaagac agctagcaat
     2281 atcatgttta tgcttgattt ggggaatcat ttagatcagt ggtccttgaa aaaaactgca
     2341 acagatttag aacagagtct tcttgcaaaa gagagcgatg tattcctagt acagggcgat
     2401 acggttgtta gtatcaagag ttccgatgtt caaataggag atgtcttgat cttatctcaa
     2461 ggaaatgaaa ttctgtttga tggacaagta gtttcaggtt taggtatggt caacgaaagt
     2521 tccttgacag gagagagttt tccagttgaa aaaagagagt ctgatttggt ttgtgcaaat
     2581 acagtattag aaactggaga gttacgcatt cgtgtaacag ataatcagat gaacagccgt
     2641 attttacagc tgattgagtt gatgaagaaa tctgaagaaa acaagaaaac gaaacaacgc
     2701 tatttcatca agatggcgga taaagtcgtc aaatataatt tcttgggggc tgggctgact
     2761 tacctattga caggttcttt ttctaaggct atttctttcc tattggtcga tttctcctgc
     2821 gctttgaaaa tctctactcc tgtagcttat ttgacagtta tcaaggaagg gttgaaccgt
     2881 gaaatggtga ttaaggatgg agatgttctg gagaaatatc tggaagttga tactttcttg
     2941 tttgataaga caggaacaat cacaactagt tatcctatag ttgaaaaggt gttacctttt
     3001 ggagactata gtgaggaaga tattctcaga atcagtgcct gtcttgagga acacatttat
     3061 catcctattg ctaatgccat cgtcaagcaa gctgagatag agggaattga acatgaggaa
     3121 atgcatggga aactccaata tatcgcaagc aaggggatca aatctcatat agatggccaa
     3181 ccagttctta ttgggaatta tgtcttgatg caggatgagc agattcatat cagttcagaa
     3241 caaaatgctt taattgaaga gtacaagagt cactacaatc tcttattctt ggcttatcaa
     3301 aatgaattga ttggaatgtt ctgcattcat actcctttga gaaaagaagc aaaaacagcc
     3361 ttggataaac ttaaggcaca ggggaaaaaa ttgattctgg caacagggga caccttggtt
     3421 aggacaaagg aattagtcaa agatttgccc tttgatcagg tctatacaga cttgaaacct
     3481 gatgggaaat ttgagttagt agagaaactg cagaaagcag gtcacactat tttgatggtt
     3541 ggagatggat tgaatgactc agcggcttta actctatcag atatcggtgt ggtgatgaat
     3601 gagagtgcag atatttctaa gcagatgagt gatatcttat tgttagataa tcgtttggat
     3661 ttcttccaag agttggattc gttatcatca tctttgcaaa cactcatcaa gaaaaatatt
     3721 caagataccg ttgtcgtaaa tagtagtttg attggcttcg gcttatttaa ttggctcagt
     3781 ccttcaaatc tctctatctt acataatcta acaaccttgc gcattgtcct gcgtagcctg
     3841 tctattaaaa atagatagat gagagttatt aacagcaaaa aggagttacc tttctcgagg
     3901 ttaactcctt tgtttataga taaatgttga atagtttagt aagtcaatac aaagtatttt
     3961 ttcttcccac gacggataac agtcagttca ttctctaact tatcagcgtc actcaagaca
     4021 tagtcaagct cttggatgcg gtcgccgttt acgtagatgg ctccgttttg gacgtcttca
     4081 cgggcttggc gttttgagtt aactatacca gatgagacga gcagttccac gatattgttg
     4141 ttttcgtctg cctgtacttg gtagtttggc acaccacgaa gtccttgttt gagctctttg
     4201 acagaaaggt ttttgatgtt tcctgcaaag agttgctcag tgatgttaag tgcttctttg
     4261 taggcttctt ctccgtgaac aagtgtaaca acttcacgag ccaagacttt ttgagccaag
     4321 cgttcgtgtg gcgctgcttc aaattgttta cgaatatctt caatctcatc aagtgacaag
     4381 aaagtaaaga ttttcaagaa gcgaacagcg tcagcgtcca tcacgttcat ccagaattgg
     4441 tacatttcgt atggagaagt cttttcggga ttgagccaga cggcatttcc ttctgattta
     4501 ccaaatttct taccagttgc atctgtgatt agtggaacag tgataacgtg accagtcttg
     4561 tccgccttac gacgaagcaa ttcggtacca gctgtcatat ttccccactg gtcagaacca
     4621 ccgatttgaa gagtgacatt atggtcttgg ttaaggacga agaagtcata cccttgcatg
     4681 atttggtaag cgaactcagt gtaagaaatt cctgtttcga tccgtttttt aacagattcc
     4741 ttactcatca tgtagttgac cgtgaagtat tttccaatat cacggaggaa gtcaatgaag
     4801 ctgatgctgc caaaccagtc gtagttgttg accatgacag ccttgttttc gccattttca
     4861 aagtcaagaa aacgagaaag ttgtccttgg atagacttga cccagccatc tactgtgtct
     4921 tttgtttgga gactacgttc agcatctttg aaggacggat ctccgatgag acctgtagca
     4981 ccgccaacga gcgcataagg tttgtgacct gctagttgca agcgacgact tgtcaagatt
     5041 gcgacaaggt ggcctaggtg aaggctgtca gcagttggat cgtagccagt ataataagaa
     5101 acttgacctt cttctagggc tttacgcaaa gcttcttcat cagtcgtttg aaatatcaaa
     5161 ccacgctctt ttagctcatc aaaaatgtgc atgtgttttt tctccttttt aaaattattg
     5221 tttctaccta ttgtatcaca aacttgggca atagcctagc agaataggga agaaagtggc
     5281 tattttattg aaaatttccc ttttgtggta taatagataa agtgaggaaa tccatgcaaa
     5341 atcaattaaa tgaattaaaa cgaaaaatgc tggaattttt ccagcaaaaa caaaaaaata
     5401 aaaaatcagc tagacctggc aagaaaggtt caagtaccaa aaaatctaaa accttagata
     5461 agtcagccat tttcccagct attttactga gtataaaagc cttatttaac ttactctttg
     5521 tactcggttt tctaggagga atgttgggag ctgggattgc tttggggtac ggagtggcct
     5581 tatttgacaa ggttcgggtg cctcagacag aagaattggt gaatcaggtc aaggacatct
     5641 cttctatttc agagattacc tattcggacg ggacggtgat tgcttccata gagagtgatt
     5701 tgttgcgcac ttctatctca tctgagcaaa tttcggaaaa tctgaagaag gctatcattg
     5761 cgacagaaga tgaacacttt aaagaacata agggtgtagt acccaaggcg gtgattcgtg
     5821 cgaccttggg gaaatttgta ggtttgggtt cctctagtgg gggttcaacc ttgacccagc
     5881 aactaattaa acagcaggtg gttggggatg cgccgacctt ggctcgtaag gcggcagaga
     5941 ttgtggatgc tcttgccttg gaacgcgcca tgaataaaga tgagatttta acgacctatc
     6001 tcaatgtggc tccctttggc cgaaataata agggacagaa tattgcaggg gctcggcaag
     6061 cagctgaggg aattttcggt gtagatgcca gtcagttgac tgttcctcaa gcagcatttt
     6121 tagcaggact tccacagagt cccattactt actctcctta tgaaaatact ggggagttga
     6181 agagtgatga agacctagaa attggcttaa gacgggctaa ggcagttctt tacagtatgt
     6241 atcgtacagg tgcattaagc aaagacgagt attctcagta caaggattat gaccttaaac
     6301 aggacttttt accatcgggc acggttacag gaatttcacg agactattta tactttacaa
     6361 ctttggcaga agctcaagaa cgtatgtatg actatctagc tcagagagac aatgtctccg
     6421 ctaaggagtt gaaaaatgag gcaactcaga agttttatcg agatttggca gccaaggaaa
     6481 ttgaaaatgg tggttataag attactacta ccatagatca gaaaattcat tctgccatgc
     6541 aaagtgcggt tgctgattat ggctatcttt tagacgatgg aacaggtcgt gtagaagtag
     6601 ggaatgtctt gatggacaac caaacaggtg ctattctagg ctttgtaggt ggtcgtaatt
     6661 atcaagaaaa tcaaaataat catgcctttg ataccaaacg ttcgccagct tctactacca
     6721 agcccttgct ggcctacggt attgctattg accagggctt gatgggaagt gaaacgattc
     6781 tatctaacta tccaacaaac tttgctaatg gcaatccgat tatgtatgct aatagcaagg
     6841 gaacaggaat gatgaccttg ggagaagctc tgaactattc atggaatatc cctgcttact
     6901 ggacctatcg tatgctccgt gaaaagggtg ttgatgtcaa gggttatatg gaaaagatgg
     6961 gttacgagat tcctgagtac ggtattgaga gcttgccaat gggtggtggt attgaagtca
     7021 cagttgccca gcataccaat ggctatcaga ccttagctaa taatggagtt tatcatcaga
     7081 agcatgtgat ttcaaagatt gaagcagcag atggtagagt ggtgtatgag tatcaggata
     7141 aaccggttca agtctattca aaagctactg cgacgattat gcagggattg ctacgagaag
     7201 ttctatcctc tcgtgtgaca acaaccttca agtctaacct gacttcttta aatcctactc
     7261 tggctaatgc agattggatt gggaagactg gtacaaccaa ccaagacgaa aatatgtggc
     7321 tcatgctttc gacacctaga ttaaccctag gtggctggat tgggcatgat gataatcatt
     7381 cattgtcacg tagagcaggt tattctaata actctaatta catggctcat ctggtaaatg
     7441 cgattcagca agcttcccca agcatttggg ggaacgagcg ctttgcttta gatcctagtg
     7501 tagtgaaatc ggaagtcttg aaatcaacag gtcaaaaacc agggaaggtt tctgttgaag
     7561 gaaaagaggt agaagtcaca ggttcgactg ttaccagcta ttgggctaat aagtcaggag
     7621 cgccagcgac aagttatcgc tttgctattg gcggaagtga tgcggattat cagaatgctt
     7681 ggtctagtat tgtggggagt ctaccaactc catccagctc cagcagttca agtagtagtt
     7741 ctagcgatag cagtaactca agtactacac gaccttcttc ttcaagggcg agacgataat
     7801 tctctaaatg aagtggccaa tcaatggatt gccacttttt tcttttcgtg ttacaataag
     7861 ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct cctatccggc
     7921 ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta tcaggtgtct
     7981 tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact catttggtct
     8041 gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa ttacctgccc
     8101 tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta tctctgtgta
     8161 gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt ctttatcctc
     8221 ttttacaatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt ctatgttttg
     8281 gttgccatgc tgggtgtttg cttgatggcg acaaagggag acctctctca gttatccatg
     8341 acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt taatgttatc
     8401 ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg ggggatgatt
     8461 ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt tactcttgat
     8521 atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt tgcttttttc
     8581 atttccatga aggctgtgtc cttggtttct cctttggtgg ttgccgttat cagtgccagt
     8641 gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt ggattggtcc
     8701 ctccttctag ctatagcctt gattatttta cccatgatat ttttgtctat agaagaagcg
     8761 aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact agaatagtca
     8821 ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta ggggcttgct
     8881 tttaccgaca gcacgtttta tctcacatcc ctaaaaaatc atacctaaaa acagacaaaa
     8941 aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga accaggaggc
     9001 acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg ccaatgctga
     9061 aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg tgcctagctc
     9121 tgttgtcaaa ctaggaaatg tcctattcgg agactggaag gacgtggctc cgcttcttga
     9181 aggtttggta gaaaatcaag attatgttgt cgagcaagat gctcgtaatt ctgcagttcc
     9241 tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta tccgtgacca
     9301 ggtggaaatt ggtgacaatg ctgttatcat gatgggagct gttatcaata tcggtgctga
     9361 aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg ccatcgttgg
     9421 aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc cagctagtgc
     9481 tgaaccagtc cgtgtcggag acaatgttct catcggggct aatgcagtgg ttatcgaagg
     9541 agtccaaatc ggtagtggtt cagttgtcgc agcaggagct attgttaccc aagatgtccc
     9601 agaaaacgtg gtagtagcag gtgttccagc tcgtattatc aaagaaattg atgcccaaac
     9661 tcaacaaaaa acagcgctag aggatgcgct tcgtaccttg taattgtaaa agtaaaaaag
     9721 aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag atgttagatt
     9781 tgattcagac tagacgagat ttacaccaga ttccagagat tggcttggag gagttcaaga
     9841 ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat tttgttcaaa
     9901 ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg gagcgaacca
     9961 ttggttggcg gacagatatt gatggcctgc ctatcgtcga acaaacaggt ctaccttttg
    10021 cttctcaaca ccaaggtcgc atgcatgcct gtggccatga ttttcatatg accattgcct
    10081 taggctgtct tgagcgcgcc cttgaggagc aacccaagaa taatctgctc tttctatttc
    10141 agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggacgat gcttttggag
    10201 attggttgcc agatcaattt tatggcctcc atgtccgtcc agatttgaaa gtcggccaga
    10261 ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc cgtttcaaag
    10321 gcaaaggtgg ccacgcagct tttcctcatg aagccaatga cgccttggtg gcggctagtt
    10381 actttgtgac ccaagtgcag tcagttgtta gccgcaatgt caacccaatc gagggagcag
    10441 tggtgacctt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca gacacagctt
    10501 ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg caaaagaggg
    10561 taaaaacagt tgcagaaggg gttgcagctg cctttgatat ggaagtcgaa gtggaactca
    10621 aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa ctgatggact
    10681 tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg acaggcgagg
    10741 actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt atcgatagtc
    10801 cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct attggggtgg
    10861 ctgcggtgtc tagtttcctg aaaaagaagg cagcagaata gagggcttgt ctatgaaatc
    10921 ggaattacgc aagcaagtct tgcatgaaat gaaggctata tctcaagagc aaaaacaggc
    10981 tatagaccaa gctttaaccg agcgaatttt acaacacccc ttttatcaag aagccaaggt
    11041 catcgcaacc tacctctctt tttctcatga gtttcaaacg cgggaactga ttgagcaggc
    11101 gctgaaggac ggcaagaagg ttttaatacc caaaacttat cccaaggggc gcatggactt
    11161 tgtagtctat gatccgcagc agttggtaaa aactgccttt gccttactgg agccacaggg
    11221 agatttggaa gtggtggatg catctcagat tgatttgatt catgttcctg gcctggcttt
    11281 tacgacggat ggatatcgga ttggatatgg tggaggttat tatgaccgct atctggaaca
    11341 tttttctggt catactttga gtacggttca tccttgtcaa attcaggact ttatacctga
    11401 aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt gatagacgtt
    11461 accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta atgttggtca
    11521 ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc atgtatgggc
    11581 cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt tttgttcata
    11641 ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga aggcaggtag
    11701 aggagatttt cggttctaag cagtttttct ttctctatct tttatcagga atgatgggca
    11761 atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct acctctcttt
    11821 atgggctatt tgccgcgatt attgttcttc gctatgcaac tcgcaatcct tatatccaac
    11881 agctagggca atcctatctg acactttttg tggttaacat tattggaagt gttctgattc
    11941 caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt ctagcagtta
    12001 tctttccagt tagaggagaa aaacggatgt ataacaccag ccagagatta ggagcggtag
    12061 tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg tgacgaagtt
    12121 gaaagaaaat ttgtgtaatg aaaaaagata aggcattttt tgaaccttat ctttttattt
    12181 tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc ttcaaatcat
    12241 ctttgacttg tgggtgtttg agggcgtagt cgatggatgt tttcatgaag ccaaacttgt
    12301 ctccgacatc gtaacgagcc ccattgaact cacgagcaaa tacacgttgt gttttattga
    12361 gggtgtcgat tgcatctgtc agctgaattt catttcctgc acctggagct tggttttcga
    12421 ggatttggaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg tcgctaggag
    12481 cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca tcttttcctt
    12541 cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact ggcatgacag
    12601 cgatagtaga cgcgtgggta cgctcgtagt catccatgag ttgtttggta agtggaacag
    12661 ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa ggttcatttc
    12721 cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga gtttggcgga
    12781 taaaatgcag ccgcatgtca gttgttttat caactagctt caaaagatct gttttccctt
    12841 tttctttgag gttatattcc aattcgaagt ttgaatcgaa gtggtcctca atagaacgtt
    12901 ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc tcttccacga
    12961 taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg gccttggttg
    13021 ctggtaaaaa tcgagttcct agtccagcag cagggataac tgcctttcta acttttgatg
    13081 tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta aattcattgt
    13141 tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact tggtaaatag
    13201 cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct gctcgagtcg
    13261 ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg gattctcctc
    13321 gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc gttacgatca
    13381 aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt gctaccccta
    13441 ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta tcaccaaatc
    13501 caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca ccagcagttt
    13561 caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta aatagctcct
    13621 gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt aggtcacgca
    13681 caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta cggagatgtt
    13741 caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt tctaatccct
    13801 ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc tgggcaacaa
    13861 gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatttttc aatgtttctg
    13921 ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag tagtgcttat
    13981 tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa atacgtacct
    14041 cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca ggccccaaga
    14101 cggcgacggt ttgtttttcc atgatttcct ccttgataga tttctttctc ttattctatc
    14161 atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata cacaaagacc
    14221 gagatgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg atagatagtt
    14281 tgattcagta tatgcttata atagaggtaa acaactcagg aagttcttgt aggttgcgag
    14341 agtttggcga aaaaattaaa agattacgtt tggctaaaaa aatcagtcgt tcagaatttt
    14401 gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat ggagaatcca
    14461 gaccaacact aacaaagtta aaatatattg ctgaacgttt ggaggttgaa gattacaagt
    14521 tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat ttcttgatga
    14581 ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt tttgataaga
    14641 tttttgaaga gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt
    14701 catatctagc actagcgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac
    14761 tgtccttctg gtgattttgc ttaatttctg atgttcgaga catgattgag gagatttgtc
    14821 ttgatttttt cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat
    14881 aaggtgactt attctgttag gcagttttcc cctgccctct agctctatat ggaacaggga
    14941 gtatcagatt tatctggcag attgtatcaa ataaggcttg aaactgtttt gaataggcgt
    15001 atagacttga gaatttgaga atatgtcgtc tagcagtaga gacatatttt ccggcaatat
    15061 gaaggaagag acgtcgaaaa cgcttgatag tcaaggcctt tacttcatca ccagctagct
    15121 gctttaaaaa gaggtagaga ttataagcca gacagcccat catcatacgt acttcattct
    15181 taatcatggt cgaactatct gtcttatccc caaagaatcc tgctttcctt tctttgataa
    15241 agttttcagc attccctcgt tcacgataga attgaaacag ctcctcacta tctacactgc
    15301 ttaaattggt caaatgaact tttttacaac cataaaaaag agcacttaat cgtactcttc
    15361 tcttttaagg attagttgaa ttaattcaaa tccactagtt ctcctgtttc taggtagact
    15421 aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt ccagatgccc tatgatataa
    15481 aggtattgag ttccattggg aattgaggtt tcttggtctt tcataagtcc aatgatttcc
    15541 tttgataagg cataataata ttggtcaatc tgttcatctt tttgagcaat actaatagct
    15601 tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg ctagcatgct gagggataat
    15661 ttacccattt ggtgtaactg ttcttcgtca ggggctagtt gattttcttt tagttgcaaa
    15721 acagctttgg caatgcctgc catatggtct cccatacgtt caaggtctga acaagaagac
    15781 atgatgctaa tcacaaatcg aaggtcagac acttgtggct gctgcaaggc caacaaacgg
    15841 gcacaggtca attcgatagc gctttgacct tggtttatag catgatcctt attgataatt
    15901 agctctgcca tctccttgtc tttggaggct aaggccagta aggcttttga agctgtttca
    15961 aggacaagtt gccctagtcc taaaaaggat tgttctaatt catgcaattc taagtcaaat
    16021 tgatttctca taggtttttc cttccttatc cgaaccgtcc tgaaatatag tcttctgtgc
    16081 gctgatcttt tggattggta aacacgtcaa cggtatctcc aaattcgcaa atttctcctg
    16141 ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg ttgcatgtta tgggtaacaa
    16201 tgataatcgt ataatccttt tttagttgct gaatgaggtc ttcaattttt aaagtggaga
    16261 tagggtctaa ggctgaagtc ggctcatcca ttaacagaat atcaggttct actgctaaag
    16321 ctcgcgcaat gcaaaggcgt tgctgctgac cgccagataa ggacatggca ctctttttaa
    16381 gatcatcttt gacttcttcc caaatggctg ccccttttaa agatttctcc actaaggcat
    16441 ctaattgttt tttgtctcga ataccatgtg tccttgggcc ataagccacg ttatcataga
    16501 tagacatggc aaagggatta ggctgttgaa aaaccatccc tacacgctta cgtagctgat
    16561 taaggttgaa tttgctacta taaatatctt gctcatctaa gaggacttgg ccttcaatat
    16621 ggcaagaagg aaccaaatcg ttcatccggt taagggtttt tagaaaagtt gatttgccac
    16681 aaccagatgg gcctatcaag gcagtaatct gtctttctgg taattgaatc gaaatatttt
    16741 ttaaggcttg aaaatccccg taaaataagt ctaggtgtct gactgaaaat gttcccatac
    16801 taggaagctc ctttcacaag ttttcgagat aataagcttg atagagtatt tatcattaaa
    16861 acagtaataa tcaaaatcac accggtagca taggcttcat tgacatgtag cccctcactt
    16921 gacagcatat acatatgcag ggctagagaa cgacctgaag acatgagact acttggcgta
    16981 ttggtagagg tacctaatgt atacatgagg gcagctgtct caccaacgat acggccaata
    17041 gctagtatca ctccagctaa aatacctggc atggcaactg gtagaacaat tctaaaaaca
    17101 gtccgtaact tacctgcccc aagtccataa cttgcttgac gcatgctatc actaacagat
    17161 aaaagggctt cttctgttga gcgaataatg actggcaaca ccatgatgac tgaggttaag
    17221 attcctgata acagagagta ttgaaaacct aagaagacta caaagaagag catgccaaac
    17281 agaccaaaaa caatggaagg aatcccagac aaggtatctg aggccaatcg catgatttta
    17341 acacaaaggg aatctttttt tgtatattcc acaagataaa aaccagcaaa aatccctatg
    17401 ggcaaggcta aaagaagagc accaaagacc agaataacgg tggaaataat cgctggcata
    17461 agggaaatgt tctcagaagt ataagtccaa gaaaagaggg atagacttag atgaggtaaa
    17521 cctttgatga ggataaaacc aatgattaaa aagagagagc caaaggttaa agctgaaaaa
    17581 caataaacga gaagttttag caggtattta ctcataagat gattttcctt tcaagtaggc
    17641 aaagtaggca ttaatcaaga gaataaggaa aaagagaact gctgaggttg caataagggc
    17701 ttccctatgc tgacctgatg cgtaagccat ttccagaaca atattggttg ttaaggttct
    17761 ggttcctgaa aagagtccac ttggaataat cggctggttg cctgccacca aaataactgc
    17821 catggtttca cctactgcgc gaccgatgcc taaaataact gctgataaaa taccagatct
    17881 cgcagctggc aagatgacac taaaaatact ccgttcatga ctagctccta gagccaagct
    17941 accagaataa tacgttttgg gaactgttcg gatagcagat tctgacaaac tgataatggt
    18001 tggcaaaatc attattccta atagtaacga agcggttagg acactcatgc catttcctaa
    18061 aaagcttcta atccaaggca ccaataattg taggccgaaa aaaccataaa caatagatgg
    18121 aatggccgcc atcaagttga tagctgattt taagaagcca tagacgggct ttggacaata
    18181 ataaaccata aacaccgatg tcaagatgcc tgttggcacc ccaatcacaa tcgctcctaa
    18241 ggtaattaat aaggaaccaa cgatcattgg taaaatacca tagcttgccg gaatgttcgt
    18301 tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg tagttagcca tgaaaggtaa
    18361 gccattacta aaaataaaga aacagattag caaaatagct acaacagcta ctgttgcact
    18421 catgaaaaaa attgccctaa aaactgcttc tttgaaggct tgttttatca catcttgtcc
    18481 tttctagtga agaaagtaag ggagatacga cacctcccta cttgccttct ttatcttatt
    18541 gtacgatgaa acgtctgcat ctctttagag atttatggag caaacatttt atttaatctt
    18601 gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt tcagccatac tgacttggct
    18661 tgccttattg tcattattga ccacaacagc aataccgtct aaagcaatag catcatgggt
    18721 gagactctta ccttcttcag gagttaattc cctagaaacc ataccaatat cagcggtttt
    18781 ctccttaaca gcggtaatac ctgctgaaga cccattagag gtaatatcaa tcgtaacttc
    18841 tggattttct tttttataag cttctgctaa tttttccatt aaagaagata ctgaagtgga
    18901 acctacaaca gacaacttgc ctgataagtg ttggcttgta tattctgtgg tttcagtttt
    18961 agcttcaata aatttattat ctgtgaccac ttgttgacct tgtttggagt ggataaagct
    19021 gataaaatct tgacctagct tggaaagatt agaagaccaa acaatgttga agggacgttg
    19081 aagagggtat tcaccatcta aaactgtgtc tcgactagcc ttgacaccat caatctctaa
    19141 agccttgaca gatttcgtta aagatcccaa ggagatgtag ccgatagcat tagcattccc
    19201 ttgaactgct gagagaacac cttctgtact attttgaatc acagctgttt tggcagtgtt
    19261 gtcaattttt ttatcaccgt cttttttgag aatccctgtg atttctgtga aggcaccccg
    19321 tgttccagag ccattttctc gtgaaatcac ctcaatcgtt cctgaagctg actgtttgga
    19381 agcagctgac tgattgccac aggcaacaag cccaaatcct gataagccaa tggctgcaag
    19441 agtaagcatt tttttgaatt tcataataat cacctttatc tctatgtatt tttcttgtgt
    19501 aggcttacta catttatagt ctaacaagtc tttgtaaagg tttatccctg attcatgtaa
    19561 agattgtgta aagaatcaaa aaaagccact tttgaaaaat ggctgctcct aaaaatagct
    19621 ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag aaaaatcgtg aagcaactgc
    19681 ctctgccaag ctgactcgtc accgtgactt ggccacctaa taattgactg agttctttga
    19741 caatggcaag gccaagacca gtgccaccag tttgtctgct tcgaccttta ttaactcggt
    19801 aaaaacgttc aaaaatacga tcctgctcta attgactaat accaatccct gtatctgata
    19861 cagaaatctt aatgccttcg ttcacctttt gggtcttgac ctcaattttt cccccttgtt
    19921 cagtgtaacg gatggcattg gataaaagat tgagtaagat ttgggaaagt aattgactat
    19981 ctgatacgag ggtgacatca tctggcacct gcacctttag ctgtaaatcc ttcttcttga
    20041 gctgaggttg caagctttga gtcaaatcct gtacaaattc agccaaagaa agggtcgtcc
    20101 attgtatagg catttgttga gccttagata aggtaagaag atgctcaaca atatgctcaa
    20161 gacgcaaact ttctttgtaa ataatgtcta gaaagtcatc cttgagcgct tcttcttcag
    20221 ctgacatccc cttaatggtt tcagcaaagc ccttaattga agtaactggt gtcctcaatt
    20281 catgggaggc atttgagaca aaggctaaat ttaacttttc ataagttcta atcgttgtta
    20341 aatcatatag caagacgagc acagcttcca cagattgggt ggggctaaaa acgggaactg
    20401 ctgtcacttc taaaatcaag tcaccctcat gaaacccact tacttcttgt tttaaccttg
    20461 ttttttgatc aaaggcttgg tgaactaaat tccgaatatc catccgtttg aggtcatcaa
    20521 gtgaacttat gtcgccgtcc acatcgggaa aataatgagg cagagagcga ctggataata
    20581 acatctgacc ttgagcggaa actaaaaacg tccccatggt taggtgcgac agaagaacct
    20641 ccattgtttc ggctaggtcc ttgtattgct gatcctgttg ggagactttg gtttttaggc
    20701 cagacacata ctgagccaaa gactttaagt cttcttgccc tttttctaaa aagtattcac
    20761 tactggtcaa gagaggttgg tgcaaggtct caaaagcaac ttcccatttc caaaggcaaa
    20821 agagccagta gccaccttgt cccaaagaaa gggctagaag aaagagaccg atgcctttac
    20881 tgatccaagt taatgccatc cctgcaatca gaatgaggct aacacttaga ttgactagcc
    20941 aaaattgaag gtagcgtttc atctataact ccttgaactt ataaccataa ccccgaatgg
    21001 ttcgaataaa ttgaggggct ttaggattgt cttcaatttt ttccctcaac ttaccaatat
    21061 gaacgtccac caaacgtgtt tcctgcccaa agtcataccc ccagatacgt tccaaaagac
    21121 gctctctagt cagtgtcatg ttgggatgtt tcataagata gagcaagagt tcaaattctt
    21181 ttggggtcaa actcagtaac ttattcgcct tgtagacttc atgacgctca gggtatactt
    21241 tcaaggtccc aaatagccaa gaatcgtcag cgatattatc tgaatcatct ccttcttgtt
    21301 ctcctttagt tcgcctgagg acagccttga cacgcgccag caattctcta gggctaaaag
    21361 gcttggtcag gtagtcatca gcccctaatt ccaaggccaa aaccttatca aattcatcac
    21421 ttttcgcaga aaccatcata attggagttt tgacgccttt ggctctcagc cgcttacaaa
    21481 cttccatgcc atctaattgt ggtaacatga tatcaagcaa gataaaatca aagggttctg
    21541 tttctgccaa agctaaggcc ttccgtccat ttgttaccaa ttgagtagaa aagccttcct
    21601 tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc atcatccact aataagactt
    21661 gttttgtcat ctattatctc ctattggtaa cattataaca caattatcag aaatcctaac
    21721 attgctaaat cagattaaat ttgcttatca agactagtat ctggtcaaac gctcaatcat
    21781 ctccttgtgc tctggatagg tcgccagtag atctaccctt tcaaataatt caaaatcctc
    21841 aaattcaaaa ccaggagcaa caagacaaga aaccagagca taatccttat caactgttga
    21901 tccccaaata gtgcccttag gaacacagta gtgaagttgt tgccctttgg atatgtccag
    21961 gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc atgtgaacag taagtgggga
    22021 tcctgcatga aaataccaga tttcatctgc tgtcaatcgg tgaaaatgtg aaggattcgt
    22081 ttcttctaat aagaaataaa tactggtata aagcgccctt cccttaccag caaggtttat
    22141 agtgtctgaa gctttttttg tttgtctaaa atagccacct tcaacatggg gagctaactc
    22201 tagagttctt atcaagtctt ctttatccat cggggccaat gggttgaagt aactcttgtt
    22261 caaagtggtt ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
    22321 gcgacggaag tacaaatcaa tcgccctaaa aaaagaatta gcgaatgatt ctggtaaaaa
    22381 gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttctat
    22441 tgccatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc tggtaaaatc
    22501 ttggctgaca gtgattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    22561 aaatccagca aactcaagcc gctaacagct gaagataaag cctataacca tgcgctatct
    22621 aaggaaagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatattttca
    22681 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    22741 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt aggtgaatta
    22801 gtgaagtgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat cgattatatt
    22861 taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta gcgtctaaca
    22921 attaggaact ttagttccaa taactttaag attacgacgt tttaggacat aaatcgatca
    22981 tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat ttggcgttag
    23041 ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc tggcgcttct
    23101 actccaagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct gagggctaga
    23161 cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta tttgttaaag
    23221 gattgagcca ggctaattgc aaatttagca atgatagaag gttcaaagtt atctgccgca
    23281 cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct ttcagtatca
    23341 ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa gatagattgg
    23401 atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga taccatagct
    23461 tctaggtcga agtcgtatcc atttgtacgg tcagttttga ggtcatagaa tttaatggct
    23521 ccaaccccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt tttagcctcg
    23581 atttggacct tggcacggct aacagcctct gcaacagtag gctctagcaa gatgacattc
    23641 cctttacgag tagagagctt cttcccttct tttgtaacca gaccaaaagg aacgtgggta
    23701 atgtcgtcac tccaatcgta gcccatctct tgcaagacag ctttgagttg tttaaagtgg
    23761 gcagattgct cttgaccaac gacgtagata gatttagcaa attggtattc atttttacgg
    23821 tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga cttcttgatg
    23881 agggctggat gttcaattcc atatttctca agattcacaa cttgggcacc ttctgattca
    23941 agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc attgtagaag
    24001 gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg gttaaattcc
    24061 actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc tccattttca
    24121 agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc agcttcagcg
    24181 ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac agcttcttcg
    24241 tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca gtctcccaaa
    24301 tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa gctatctccg
    24361 ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg actagacatg
    24421 tcgataacaa cattttcttg tttgccaata ttttggtcag catagtgttc tttttcagtg
    24481 gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa gttaacgtaa
    24541 ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc cagttcagcc
    24601 gcaatcattt gtggtgcttt acgttcgact ttggcaagag aaaaagcagg gaaagcaatg
    24661 tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc ttggtccagg
    24721 ctatcaatga tgctagataa ttcgctagca atcaattctt ttgtattcat taagagct
//
LOCUS       DAVLFB010000022        24412 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLFB010000022 DAVLFB010000000
VERSION     DAVLFB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.022"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(107..856)
                     /locus_tag="VXD58_000782"
     CDS             complement(107..856)
                     /locus_tag="VXD58_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV0675521.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            989..3325
                     /locus_tag="VXD58_000783"
     CDS             989..3325
                     /locus_tag="VXD58_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV0675522.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLMETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDSIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3427..4104
                     /locus_tag="VXD58_000784"
     CDS             3427..4104
                     /locus_tag="VXD58_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV0675523.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4091..4888
                     /locus_tag="VXD58_000785"
     CDS             4091..4888
                     /locus_tag="VXD58_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV0675524.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4905..6008
                     /locus_tag="VXD58_000786"
     CDS             4905..6008
                     /locus_tag="VXD58_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV0675525.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            6309..7328
                     /gene="galE"
                     /locus_tag="VXD58_000787"
     CDS             6309..7328
                     /gene="galE"
                     /locus_tag="VXD58_000787"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0675526.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7330..8283
                     /locus_tag="VXD58_000788"
     CDS             7330..8283
                     /locus_tag="VXD58_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0675527.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(8345..8551)
                     /locus_tag="VXD58_000789"
     CDS             complement(8345..8551)
                     /locus_tag="VXD58_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV0675528.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8603..9079
                     /locus_tag="VXD58_000790"
     CDS             8603..9079
                     /locus_tag="VXD58_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV0675529.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9088..9759
                     /locus_tag="VXD58_000791"
     CDS             9088..9759
                     /locus_tag="VXD58_000791"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV0675530.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9890..10228
                     /locus_tag="VXD58_000792"
     CDS             9890..10228
                     /locus_tag="VXD58_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV0675531.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10231..10860
                     /locus_tag="VXD58_000793"
     CDS             10231..10860
                     /locus_tag="VXD58_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV0675532.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11100..12251
                     /locus_tag="VXD58_000794"
     CDS             11100..12251
                     /locus_tag="VXD58_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0675533.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12564..13313
                     /gene="truA"
                     /locus_tag="VXD58_000795"
     CDS             12564..13313
                     /gene="truA"
                     /locus_tag="VXD58_000795"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV0675534.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13303..14076
                     /locus_tag="VXD58_000796"
     CDS             13303..14076
                     /locus_tag="VXD58_000796"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0675535.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14054..14515
                     /locus_tag="VXD58_000797"
     CDS             14054..14515
                     /locus_tag="VXD58_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0675536.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            14742..16083
                     /locus_tag="VXD58_000798"
                     /pseudo
     CDS             14742..16083
                     /locus_tag="VXD58_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(16211..16414)
                     /locus_tag="VXD58_000799"
     CDS             complement(16211..16414)
                     /locus_tag="VXD58_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675537.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(16407..17489)
                     /locus_tag="VXD58_000800"
     CDS             complement(16407..17489)
                     /locus_tag="VXD58_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV0675538.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(17615..18397)
                     /locus_tag="VXD58_000801"
     CDS             complement(17615..18397)
                     /locus_tag="VXD58_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV0675539.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(18397..19740)
                     /locus_tag="VXD58_000802"
     CDS             complement(18397..19740)
                     /locus_tag="VXD58_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV0675540.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(19839..21155)
                     /locus_tag="VXD58_000803"
     CDS             complement(19839..21155)
                     /locus_tag="VXD58_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV0675541.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            21348..22574
                     /locus_tag="VXD58_000804"
     CDS             21348..22574
                     /locus_tag="VXD58_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV0675542.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            22773..24347
                     /locus_tag="VXD58_000805"
     CDS             22773..24347
                     /locus_tag="VXD58_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0675543.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         6951 a         4677 c         5575 g         7209 t
ORIGIN      
        1 agtcaaatcg atttctaaaa atgttttaga agtagaggtg tactattcta gttccaatct
       61 actatataaa tgaaaacctt agctgtttgg ccaaggttca tttcttctat gcaagttctt
      121 ctcttttctt atctgggttc cagatgaagc ttgcgataaa gctaaagatg atggttagga
      181 tagcgaggat aatagcaagg atgagggcag ggatgcggat aaaccaccag agtggatttg
      241 gttttttatc attgtgccat tgtttcgttt cttcgtccag acgttggaat tctgtttgaa
      301 tacgattggc gactgtttca atgccttcat cattcatttt cttgatatct gagatatcga
      361 ttggatttcc aaagttcata tcgacacgtt cacggctaat caagcccttc aaagtcatgg
      421 gaccggtgta ggtaaccggc atgatacgga ccttggccat tttggcaatc agtgctgcgc
      481 cccccttgac atcgtttgag tggcggctac cacttggaaa catgatgaga gagcggtcac
      541 tttttttgag aacgttgata ggatatttga tggctgaggc gctgggattt tcacggtcga
      601 tgggaaaggc gccacacata cgaatccacc aaccaaagat acggttggta aagagttctt
      661 tttttgccat aaagatgaac tgttttggct tggtcgcaaa ggccatataa acaggatccc
      721 accaggtacg gtgaggcgca actaaaatat aattttcatc ttgattagga attttatcag
      781 tattatgata gtgagcattg ccattgatgg accataagag caatacaact aatccacgca
      841 aataagtata aaacatgcga tctccttcga ttgttttctt gttattatta taccttatca
      901 aaggagggct ggcaaacttt tcccttgact agatacatat ttaggatgaa attagaattc
      961 tgttaaaaaa aatgatataa tagaatttat ggataaaaat aagattatgg gattaaccca
     1021 aagagaagtc aaggaaagac aggctgaggg tttggtcaat gactttaccg catcagccag
     1081 taccagcact tggcaaatcg ttaaacgaaa tgtctttacc ctttttaacg ctttgaactt
     1141 tgccattgct ttggctcttg cctttgtgca ggcttggagc aatctggtct tctttgctgt
     1201 tatctgcttt aacgcttttt ctgggattgt gaccgagcta cgagccaaac acatggtgga
     1261 caagctcaat ctcatgacca aggaaaaggt caaaaccatc cgtgatggtc aggaagttgc
     1321 tcttaatcct gaagaattag tgctaggaga tgtcattcgt ttgtctgcag gagagcagat
     1381 tcctagtgat gccttggttt tggaaggctt tgcggaagtc aatgaagcca tgttaacggg
     1441 agaaagtgat ttggtgcaaa aggaagttga cggcttactt ttgtcaggaa gtttcctagc
     1501 cagtgggtca gttttatctc aagttcacca tgtcggtgca gacaactatg ctgccaaact
     1561 catgcttgag gctaagaccg ttaaacccat caactcccgt atcatgaaat cgctggacaa
     1621 gttggctggt tttactggga agattatcat tccctttggt ctggctctct tgctggaagc
     1681 cttgctttta aaaggcctgc ctctcaagtc atccgttgta aactcgtcga cagctctttt
     1741 gggaatgttg cctaagggaa ttgccctttt gaccattact tcgctcttga ctgcagtgat
     1801 taagttgggc ttgaaaaagg tcttggtgca ggagatgtac tctgttgaga ccttggcgcg
     1861 cgtggatatg ctctgtctgg acaagacggg taccatcacc caaggaaaga tgcaggtgga
     1921 ggctgttctt ccgttgatgg aaacgtatgg tgaagaggct attgccagca tcttgactag
     1981 ctacatggcc catagtgagg ataagaatcc aactgcccaa gccattcgcc agcgttttgt
     2041 gggagatgtt gcttatccta tgatttccaa tcttcccttc tcgagcgacc gcaagtgggg
     2101 ggctatggag ttagaaggct tggggacagt tttcttaggg gcgcctgaga tgttgctgga
     2161 ttctgaagtc ccagaagcca gggaggcctt ggagagagga tcacgtgtct tggtcttagc
     2221 tctcagtcag gagaaattag accatcacaa accacagaaa ccatctgata ttcaggctct
     2281 agccttgctg gaaatcttgg actccattcg agagggagca gcagagacgc tggactatct
     2341 ccgttctcag gaggtaggac tcaagattat ctctggtgac aatccagtta cggtgtccag
     2401 cattgcccag aaggctggtt ttgcggacta tcacaactat gtagattgct caaaaatcac
     2461 cgatgaggaa ttgatggcca tggcggagga gacagctatt ttcggacgtg tttcccctca
     2521 tcaaaagaaa ctcatcatcc aaacgttgaa aaaagcgggt catacaacgg ctatgacagg
     2581 ggacggggtt aatgatatct tggcccttcg tgaggcggat tgttctatcg tgatggcgga
     2641 gggggatcca gcaacccgtc agattgccaa tctggttctc ttgaactcag actttaatga
     2701 tgttcctgag attctcttcg agggtcgtcg cgtggtcaat aacattgccc acatcgcccc
     2761 gattttcttg ataaagacca tctattcctt cctgttagca gtcatctgta ttgccagtgc
     2821 tttactaggt cggtcagagt ggattttgat tttccccttc attccgatcc agattaccat
     2881 gattgaccag tttgtggaag gtttcccacc attcgttctg acttttgagc gaaatatcaa
     2941 acctgttgag cagaatttcc tcagaaaatc catgcttcgt gccctaccaa gcgctctcat
     3001 ggtcgtcttt agcgtcctgt ttgtgaaaat atttgggagt agccaaggtt ggtctgagtt
     3061 agaaatctca actctcctct attatctctt ggggtcaatt ggtttcttat ccgtatttag
     3121 agcctgcatg ccatttaccc tatggcgtgt cctcttgatt gtttggtcag taggaggttt
     3181 cctagccaca gctctcttcc caagaatcca aaaactgctt gaaatttcaa ccttaacagg
     3241 acaaacatta cctgtttatg gtgtcatgat gttggtcttt accgtgattt tcatcctgac
     3301 cagtcgctat caagccagaa aataaagaaa ggctgcaatc tgtggattgc agttttttta
     3361 ggtacaagat tgccagtcga aatgtggtat aataagaggt aatagagttt tggaaagtga
     3421 gagaagatga tttcaaagag attagaattg gtagcttcct ttgtgtcaca gggggctatt
     3481 ttactagatg tgggaagtga ccatgcttat ctgcctatcg agttggttga gagaggccaa
     3541 atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct atcagtctgc ggttaaaaat
     3601 gttgaggctc acggcctaaa ggagaaaatc caagtccgtt tagccaatgg cttggcagct
     3661 tttgaagaga ctgaccaagt gtctgtcatt accattgctg gcatgggtgg tcgtttgatt
     3721 gctaggattt tagaagaagg tttggggaag ttagctaatg tagagcgttt gatcctccag
     3781 cccaataatc gtgaagacga cttgcgtatc tggctacagg atcatggatt ccagattgta
     3841 gcagaaagca ttttagaaga agctggaaag ttttatgaga ttttggtggt ggaagcagga
     3901 caaatgaagc tatcagccag tgatgttcgc tttggtccct tcttgtccaa agaagtcagt
     3961 ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc cctcggacaa
     4021 atcccagaaa aaaatctgga agaacgtcaa gttctagtag ataagattca agctatcaag
     4081 gaggtgctcc atgttagcaa gtgaagtgat ccaagcttat gaagcctttt gccctcagga
     4141 attttctatg gagggagaca gccgtggtct gcaaattggc actttagaca agggtatcca
     4201 aagggtcatg gttgccctcg atattcgtga agagacggtg gcagaggcta ttgaaaaggg
     4261 tgtggacttg attatcgtca agcacgcgcc tatctttcgt cctatcaagg acttgcttgc
     4321 cagccgtccg caaaatcaga tttacatcga cctgattaag catgatatcg cagtttatgt
     4381 cagccatacc aatattgata tcgttgaaaa tgggctcaat gactggtttt gtcagatgct
     4441 aggaatcgag gagacgactt atctgcagga aacaggtcca gaacgtggaa ttggacgtat
     4501 tgggaatatt cagcctcaga cattttggga attggcccaa caggtcaagc aagtctttga
     4561 cctagatagc cttcgaatgg tgcattatca agagaatgat ttgcagaagc ctatttcaag
     4621 agtagcaatt tgtggtggaa gcgggcagtc tttctataag gatgctttgg caaagggggc
     4681 agatgtctat atcactggtg atatctacta ccatactgct caggatatgc tgtctgatgg
     4741 cttgttagca ttggatccag gtcactatat cgaagtgctt tttgtggaaa aaatcgcagc
     4801 actccttact caatggaaag agaaaaaagg ctgggaacta gagattttac ctagtcaagc
     4861 atcgaccaat cctttccgtc atatctagtt agaaagtgaa gacaatgaaa aaagttgcca
     4921 ttattggagc aggaattgtg ggagcaacag ctgcctacta cctctcgaga gaaagtgacc
     4981 tagaggtgac cgtttttgac catgagcaag gtcaagccac caaggccgca gcaggaatta
     5041 tcagtccttg gttttccaaa cgccgtaata aagcctggta caagatggcg cgcttggggg
     5101 ctgattttta tgtggattta ttagctgatt tagagaaatc aggacaagaa atcgactttt
     5161 accagcgttc gggagtcttt ctcttgaaaa aggatgaatc caagttggaa gaactctatc
     5221 aactggctct ccagcgcaga gaagaatctc ccctgatagg ccagttagcc attctggatc
     5281 aagcatcagc taatgaatta ttccctggct tgcagggatt cgacaacttg ctctatgctt
     5341 ctggtggagc gagagtagat ggccaactct tggtaactcg tctgctagaa gccagtcatg
     5401 tcaagttggt caaagaaaaa gtgactctga cactcttatc atcaggctac cagattggcg
     5461 aagaggtgtt tgatcaggtt attttggcaa cgggagcttg gttgggggac atgttagaac
     5521 ctttaggtta tgaagtggat gtccgtcctc aaaaaggaca actacgagat tatcagcttg
     5581 cccaagacat ggaagattac cctgttgtca tgccagaagg ggagtgggat ttgattccct
     5641 ttgcaggtgg gaaattatcc ttgggcgcta cccatgaaaa tgacatggga tttgatttga
     5701 cggtagatga acccttgctc caacaaatgg aggaggcggc cttgcctcat tacccagttt
     5761 tagctagagt gacttcaagg gctgagcgtg tgggaatccg tgcctatacc agtgatttct
     5821 ctcccttctt tgggcaggtg ccagaattgg caggtgtcta tgcagccagt ggactaggtt
     5881 catcaggcct cacaactggt cctatcattg gttaccatct agcccaactg atccaagaca
     5941 aggagttgac cttggaccct ctaaattacc caattgaaaa ctatgtcaaa cgagtaaaaa
     6001 gcgaataata ctcaatgaaa atcaaagaac aaactaggta actagccgcg ggttactcaa
     6061 agcactgctt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgagg
     6121 ttgtggatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg ttgacgtggt
     6181 ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg tagtttagga
     6241 tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaataatct gagaatcgag
     6301 gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt ggaacccaca
     6361 ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat cttgtcaata
     6421 gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt cctttctatg
     6481 aggcagatat tcgtgacaca gctaccctca gagatatttt caagcaggaa gaaccgactg
     6541 gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc cctcttgcct
     6601 actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa gaaaacaact
     6661 gtaaaaacat catcttcagt tcttctgcga cagtttacgg ggatccgcac acagtgccca
     6721 tcttggaaga tttcccactt tcagtgacca acccatacgg tcgtactaag ctcatgctag
     6781 aggaaatttt gactgatatt tacaaagcag actcagaatg gaatgttgtc ttgcttcgtt
     6841 actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca aacggtattc
     6901 caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag caagtgcaag
     6961 tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgactat atccacgttg
     7021 tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt tcaggactaa
     7081 acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc caaaacatgg
     7141 aaaaagcggt gggccgtcct attccttacc gcatcgtaga acgtcgccca ggtgatatcg
     7201 ctgcctgcta ctcagacccg gcaaaagcta aagcagaact cggttgggaa gcagaactcg
     7261 acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca aatggatttg
     7321 aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga agtacttcct
     7381 cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat cgagtatgtc
     7441 tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta tcgggagcaa
     7501 aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc agccctttat
     7561 gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga cctccaagat
     7621 cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga cttggactgc
     7681 gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt ctgtgctgtt
     7741 ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg tgtccgtgat
     7801 tttcgtatga tgagaaggtc tgtagtcgat gccattttaa gcttgaccga gtccaatcgt
     7861 ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct actatttgga ctatccaaat
     7921 gtcgaaaggc aggctggcaa gaccagttgg agttttaggc aactcttttt ttactccatt
     7981 gaagggattg ttaatttttc agatttccct ttgactatag cctttgtagc tggtctccta
     8041 tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct cattttgggc
     8101 aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg aggcattcaa
     8161 ctcttgacca ttgggattct cggtaagtat atcagtaaga tttatttaga gactaaaaaa
     8221 agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga aaaaataaag
     8281 tgaaaagact ataattttac atggaaatgt gctaaactag agggagtgga ttatcgccat
     8341 tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg gcaattttta acagcctcta
     8401 agacatcctg actaggagaa atttcttttt ccagttggtc agggtcatcg taaaaacgca
     8461 cgattccatt atcgtggtaa tcaaataaat cagaataagt ttggcaaagt ccacaggcga
     8521 tacatcgttc aggtataagt gtgattttca tgttcatatt tatattgtaa taagaaaagt
     8581 aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg caagaagata tttatgatca
     8641 agaagaatca agagcagggc gtcggcatcg aaaccacgga ggggctgata ggatggctaa
     8701 tcgtattttg acgatcctag ctagtatttt ctttgtaatt gtggtggtga tggtcatcgt
     8761 tctcatctat ctatcatcgg gggggagtaa tcgcacagca gccttaaaag actttcatga
     8821 ttctgatgca agtgtagtac aaatctcatc ttcaagcagt tctcagcctg agcagagttc
     8881 agagccagaa tctacttcta gtagttcaga agaagctgct aatcctgaag gaacgattaa
     8941 agttctcgca ggagaagggg aagcagctat tgccgctcgt gcaggaatct ccattgctca
     9001 gttagaggcc ttgaatcctg ggcacatggc tacaggatct tggtttgcta atccaggtga
     9061 tgttataaaa ataaaatagg agccgatatg aaaacaattc aaattgctat tgatggtcct
     9121 gcttccagcg gtaagagtac ggtcgcaaag attattgcta aggattttgg gttcacctac
     9181 cttgatacag gagctatgta tcgtgcagcg acctatatgg ctcttaagaa ccaattagga
     9241 gttgaagaag tcgaagccct tctagccttg ttggaccagc atccaatcag ctttggacgt
     9301 tcagaaactg gagaccagct tgtttttgta ggagatgtgg atattaccca tcctatccgt
     9361 gaaaatgaag tgaccaatca tgtttctgct attgcagcaa ttcctgaagt gcgtgagaaa
     9421 ctggtttctc tccaacaaga aattgcccag caaggcggga ttgtcatgga tggtcgcgat
     9481 attggaactg ttgtattgcc acaagcagaa ttgaaaattt tcctagtagc ttctgttgat
     9541 gagagagcag agcgtcgtta caaggaaaat attgccaagg gaattgaaac agaccttgaa
     9601 accctaaaaa aggaaattgc tgcgcgtgac tacaaggata gtcatcgtga gacttctcct
     9661 ctcaaacaag cagaggatgc tgtctacctt gatacaactg gtttgaacat tcaagaagta
     9721 gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa tgtagaaaag ccgatagatt
     9781 gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt agggtataat agttgctgtt
     9841 cgagaaattt tgattttcaa agaatagtga atgatgataa ggagaaacca tgaacaattt
     9901 accaaattgt ccaaaatgta actcagagta tgtctacgaa gacggtgccc tactggtttg
     9961 cccagagtgt gctcatgagt ggaatcctgc tgaagttgca gaagtagaag aaggtcttgt
    10021 cgctatcgat gccaacggaa ataaattgac tgatggtgat acagtaactc tcatcaagga
    10081 cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga aaaatatccg
    10141 catcgtagaa ggcgatcaca atatcgactg taagattgat ggttttggtg ccatgaaact
    10201 taaatcagag tttgtgagga agatttaagc atgtcaaaac actataaact tgtattttat
    10261 agccgtatct tcttgtttct agcggctttt acgggagttt atcttgaaat tactaggcat
    10321 ggtggttttg ggatgcttct ctattacacg gttctgtcca acctcttggt aactattttt
    10381 accctttatc ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag gccaagtctc
    10441 ttgcgcttaa aaggtggggt caccatgagt atcatgatta cctgtgtgat ttaccatttc
    10501 ctcttggcgc ccattgcgac taatttctat accctagaaa atttcctttg ccactatatc
    10561 gttcccatct ggtttttagc ggataccctc ttttttgaca aacagggtca atacaagatt
    10621 tgggatccag cagtgtggac cattttaccc tttctgtata tgatgtttgc tctttttaat
    10681 ggcttggttc taaaactcaa tattccaaat gccaaggata atcctttccc ttacttcttt
    10741 ttgaatgtga acaagggttg gaatgttgtg tttaagtggt gtctgattat ctttgttgcc
    10801 tatatggtag caggatttat tttctacttt atcaagcaaa tcaagagaaa gtcatcctaa
    10861 tactcttcga aaatctcttc aaaccacgtt agtttccatc tgcaacctca aaacactgtt
    10921 ttgaacaacc tgcggctagc tttctagttt gctctttgat tttcattgag tatgagatac
    10981 aagggattca ttttcgaatt taagaaggag tctacaaacc aatgaggctt cttcttttct
    11041 tgcttatttg ataactatca aaaaagtaga agattaagag aaatatagaa agagatttca
    11101 tgaaacaatt tttagaacgg gccagcattt tggctctctc cctcgttttg attacctcct
    11161 tttccatttc gagtgcccta ccagccatgt ttgactatta tcagggttat tctaaggaac
    11221 aaattgagct cttggtgagc ttgccttcct ttggaatcat gatgatgtta ctgctaaatg
    11281 gtttcttaga aaaaatattt cctgagcgct tacagattag tttgggcttg ctgattttat
    11341 cattgagcgg tacagctccc ttctggtacc aagcctatcc ctttgtcttt ggaacacggc
    11401 ttctctttgg tttgggtctt gggatgatca atgccaaggc catttctatt atcagtgaac
    11461 gctaccaagg aaaaaggcga attcagatgt tagggctacg cgcttctgca gaggtcgttg
    11521 gagcttctct cattaccttg gccgtcggtc agttgttggc ctttggttgg acagctatct
    11581 ttctagccta tagtgctgga tttttggtga tgccccttta tctgctcttt gtcccttatg
    11641 gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc aagtcgttta actcgagaaa
    11701 tgaaaggctt gatttttacc ttagctatcg aagcggcagt tgtagtttgt accaatacag
    11761 ctattaccat ccgtattcca agtttgatgg tggaaagagg attgggggat gcccagttat
    11821 ctagttttgt tcttagtatc atgcagttga tcgggattgt ggctggggtg agtttttctt
    11881 tcttgatttc tatctttaaa gagaaactgc tcctctggtc tggtattacc tttggcttgg
    11941 ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt agtagcagga agtgttctgg
    12001 ctggatttgc ctatagtgta gtcttgacga cggtctttca acttgtctct gaacgaattc
    12061 cagctaaact cctcaatcaa gcaacttcat ttgctgtatt aggctgtagt ttcggagcct
    12121 ttacgacccc atttgttcta ggtgcaattg gcttactaac tcacaatggg atgttggtct
    12181 ttagtatctt aggaggttgg ttgattgtaa tctctatctt tgtcatgtac ctacttcaga
    12241 agagagctta ggattgattc ctaagttttt cttttgatac tcaatgaaaa tcaaagagca
    12301 tagttgattg agtttggaat agtacgctgt agctgctaaa atatttctag aaattaattt
    12361 gacttttcta atagagttat tcatatctta tttcaatcta ctatagaaag ctagccgcag
    12421 gttgctcaaa atagtgtttt gaggttgcag atggaaactg acgtggtttg aagagatttt
    12481 cgaagagtat gagattttta tacccataca atatttgttt taggtccttg tttacagact
    12541 aatttcttga taaaatagag gttatgacga gatataaagc aactatttcc tatgatggtt
    12601 atgcctttgc tggctttcag cgccagtctc atgcgcgtag cgttcaggaa gaaattgaaa
    12661 aaaccttgac cagattaaat aaagggcaaa ccattactgt tcacggtgct ggtaggacag
    12721 atagtggggt tcatgccctg ggacaggtca ttcattttga cctgccttat cagatggatg
    12781 aggagaaact ccgttttgcc ttggataccc agtctcctga agatattgat gtgatttcga
    12841 ttgagcttgt ggcagatgat tttcattgcc gttatgccaa acatagcaag acctatgagt
    12901 ttattgtgga tagaggacgt cccaaaaatc ctatgcgccg tcactatgcc acccactttc
    12961 cctacccact cgatgtggaa cgaatgcaga ttgcaatcaa aaagctagag ggaacccatg
    13021 attttaccgg ttttacagcc tctgggacta gtgtagagga taaggttcgc accatcacag
    13081 aagctagttt aatagtcgat gagacaggac aatttttgac ctttaccttt tcaggaaatg
    13141 gtttcttgta taaacagatt cgcaatatgg tggggactct tctcaaaatc ggtaacaacc
    13201 gcatgccagt agagcagatt gatctcatct tggagaagaa ggacaggcaa cttgcaggtc
    13261 ccactgcagc accaaatggt ttgtatttaa aggagattcg ttatgaagaa taatcgtatt
    13321 ttagcacttt ctggaaatga tatttttagt ggtggtggac tgtcagctga tttggctacc
    13381 tataccttga acggcttgca tgggtttgta gcagtgactt gtttgacagc cttgacagaa
    13441 aaaggatttg aagtctttcc aactgatgat accatttttc aacatgaatt agatagctta
    13501 cgtgatgtgg aatttggggg aattaagatt ggtcttctcc ctactgtcag tgtggctgag
    13561 aaggccttgg actttatcaa acaacgccca ggagtacctg tggtgttgga tcctgtcttg
    13621 gtctgcaagg aaacgcatga tgtagctgtc agtgagctct gccaagagtt gattcgcttt
    13681 ttcccttatg tcagtgtgat tacgcctaat ctcccagaag cagaattatt atccggtcag
    13741 gaaattaaaa ccttggaaga catgaaaact gcagcgcaga aattgcatga tttaggagcg
    13801 ccagcagtca ttatcaaggg aggcaatcgt cttagtcagg acaaggctgt ggatgtcttt
    13861 tatgatggac agacctttac tatcctagaa aatccagtta tccaaggcca aaatgctggt
    13921 gcaggttgta cctttgcctc tagcattgcc agtcacttga ttaaaggtga taaacttttg
    13981 ccagcagtag aaagctctaa ggctttcgtt tatcgtgcta ttgcacaagc agatcagtat
    14041 ggagtaagac aatatgaagc aaacaaaaac aactaaaatc gcccttgtat ccctattaac
    14101 cgccctttct gtggttctag gttatttctt aaaaatccca acacctacag gaattctaac
    14161 tcttttagat gctggtgtct tctttgcggc cttttacttt ggtagtcgtg aaggagcggt
    14221 agtcggagga ctagcaagtt tcttgattga cctcttatca ggctaccctc agtggatgtt
    14281 ctttagcttg gtcaaccatg gcttgcaggg atttttcgca ggatttaaag gaaaaagtca
    14341 gtggttaggc cttattttag caactattgc catggtagga ggctacgcct tgggttctac
    14401 tttgatgaat ggctgggcag cagccctccc agaaattcta ccaaatttca tgcaaaatat
    14461 ggtagggatg attgtaggat ttattcttag tcaaagtatc aagaagatta agtaatactc
    14521 ttcgaaaatc tcttcaaacc acgtcagctt ccatctgcaa cctcaaaaca ctgttttgag
    14581 cagcctgagg ctagctttct agtttgcact ttgattttca ttgagtataa agaggttgag
    14641 taaaaagtat ttcaaataag aaaaacgcat agtatcaggt gttgaatgaa ctgcccccca
    14701 aaagttagac agaaaaaatc taacttttgg ggtgttttat tatgaaacta acttatgatg
    14761 ataaagttca gatctatgaa cttagaaaac aaggatatag cttagagaag ctttcaaata
    14821 aatttgggat aaacaattct aatcttaggt acatgattaa attgattgat cgttacggaa
    14881 tagagttcgt caaaaaaggg aaaaatcgtt actattctcc tgatttaaaa caagaaatga
    14941 ttcataaagt ctgacatgaa ggctagacta aagatagagt ttctcttgaa tacggtctcc
    15001 caagtcgtac gatacttctt aactggctag cacaatacag gaaaaacggg tatactattg
    15061 ttgagaaaac aagagggaga gtacctaaaa tgggacgtaa gccaaaaaag agacctgaag
    15121 agaggacaga attagaacgt cttcaagcag aaaatgagta cctgagagcg gagaatgcca
    15181 tcctaaaaaa gttaagagaa ctccgattga aggaggaaaa agagaaagac agaaattgtt
    15241 taagaattaa tgactgagtt ttcgttagat cttcttctaa aagccattaa actagctcgt
    15301 tcgacctact actatcactt gaaacagcta gacaaaacag ataaggacca agagcttaaa
    15361 gctgaaattc aatccatttt tatcgaacac aagggaaatt atgcttatcg tcgaattcat
    15421 ttagaactaa gaaatcgtgg ttatctggta aatcataaaa gagttcaagg cttgatgaaa
    15481 gtactcaatt tacaagctaa aatgcgacag aaacgaaaat attcttctca taaaggagac
    15541 gttggtaaga aagcagagaa tctcattcaa ggccaatttg aaggctctaa aacaatggaa
    15601 cagtgctaca cagatgtgac tgaatttgcc attccagcaa gtactcaaaa gctttactta
    15661 tcaccagttt tagatggctt taatagcgaa attatcgcct ataatctttc aacttcaccc
    15721 aacttagaac aagtacaaac aatgttggaa caggcattca cagagaagca ctacgagaat
    15781 acgattctcc atagtgacca aggctggcaa tatcaacacg attcttatca tcagtttctt
    15841 gagggtaagg gaattcaagc atccatgtca cgcaagggaa acagccaaga caacggtatg
    15901 atggagtcct tctttgggat tctgaaatcg gaaatgtttt acggttatga gaagtcgttt
    15961 cagtcgctta agcaattgga acaagctatt gtagactata ttgattacta caacaataaa
    16021 cgaattaagg taaaactaaa aggacttagt cctgtgcaat acagaactaa atccttcgga
    16081 taaattaatt gtctaactgt ttgggggcag tacattttaa cacttgatac tatgcgtttt
    16141 attgtgggaa gattttcctc attttctagt gaaattgaac tttttctaac atcttttgat
    16201 agatgacttc ttaatcaatt atcatttcca ttgttgtttc ttggggtttc atcccttgtc
    16261 tttgataaaa gcggagagca ccctcgttat cattccagac acttagggtt agattattgc
    16321 agccaatttc tttagcataa tagcagaaaa atgacaataa aatagcccct agatattttg
    16381 aaaggaaagc ataggggcta tatagtttaa tcaaaataaa gcaaatcttt gctggtgctt
    16441 gtaaagaggt cgaagccatc cttggtaaca acaccgcagt cttcaatacg aacaccgact
    16501 ttaccaggga tatagatacc tggttcaaca gagaagcaca tgccttcttc gatgaccatg
    16561 tcgtttcctt ccatgataga tgggaattca tggacatcca taccgatacc atgcccgaga
    16621 cggtggttga agtactcacc ataaccagct ttttcgatga cctcacgggc agcgcggtcc
    16681 acttcatgag cagtcacacc tggcttgata aagtcaagag cagcttgttg ggcttcaaga
    16741 gtcaagttgt aaatatcttt cttgaattgg tctggtttgc cgacagcgac tgtacgagtc
    16801 atatctgacg catagccatt gaccagaaca cccaggtcaa agaggagaag agcatcattt
    16861 tcaaccttat tagctgctgg aatgccgtgt ggattcgcag cattatcacc agtcaagacc
    16921 atggtatcaa agctcatttc ataaccttca cgtttcatgg caaagtcgat ttgtgcgatg
    16981 atatctgtct cagtcttatc aagagaaata ttgtcaaaac caacatgaac agccttgtca
    17041 gcataaagac ctgcaaccat cattttttgc acttcatcag ctgatttgat gaggcgcatg
    17101 cgttggatac gaggagtgag gttgtcaaac tcagcagtct caaaaactgt tttcaaacca
    17161 tggtatttgg tcaagatgag attgtcaaac tcaacagcga cacgtttgaa gtcaagttgt
    17221 ggaagagcat gtttgatttt ttgccatgga ttttcagaat cgacatagcc cactactggg
    17281 aaggaaacgg tgctacttgc acgttctact tcaagagctg ggacaaagag gagaggttcc
    17341 tgatccgcta ggacaaagag gaacatttgg cgttcatggg gatcactgta aaaaccagtg
    17401 aggtaattga ttgtgacggg gtcagatacg acagcgacgt ctagtttttc tgattcaaga
    17461 tatgttagga tttgttgtaa tttagacata tgctaccttc tttctacccc tctattttgg
    17521 caaaaaatga gagaaaatgc aagggagaag ggtaaaagaa aagacaaaaa actccgacaa
    17581 gataacggag ttctttgata tcattttcct ttgtttaaga aaagtcagcc ttgcttttga
    17641 cgtcactgta ctcttcagcg atttcttggc tgagaatgtc ctcataggca gggagttgga
    17701 tgtcctgacc atatttcttc ttgagggcag tagtaactag gcgataagcc agattggcat
    17761 tacgagagag gataggcccg tggaagtagg aaccaaagac attcttataa tgaacccctt
    17821 caccgacctt ttcttcgttg tttccatttc catagacaac ctgtcccagc ggtttttggt
    17881 catcagagag gaaggtacga ccttggtgat tttcaaatcc atagtaggtt tcatcgaaat
    17941 cttcattgtg aatcttgatg tcaccgataa aacggttatt ggtctggttg agcgtgtagt
    18001 gtcccatgac ccctagccct tcgatacgtt ttcctgaagc ttcaacataa tattgaccca
    18061 atagttggaa accaccgcag atagccagaa ctacaccgtc gttttggatg tagttgtcaa
    18121 tgctctcttt tttagcaggt aggtcgtctg caatgatact ttgttcaaag tcttgaccac
    18181 caccgaaaaa ggcgatgtcg tagtgatttt catcaaagtc atcatggaga gaaacgatgt
    18241 caacggtcac atgggctccc agtttttcag ccacatactt gagcatgagg atgtttccat
    18301 tgtccccgta ggtattcatg agatttccgt agaggtgggc aatgttgagc tgataggggt
    18361 aattgctatc ttttgaggaa agtgaagtat aaaccattag ttcatctcct ttctaacaat
    18421 ctgacgacta gccagcagtt cacgaaattc cagcatggca gtataagttg ccagaatata
    18481 ggcatgcttg cagtcttgat tctcaatggt cttgagaact tgctccagat tactcgtttc
    18541 agtgattttc tcagctggat agccagtcac tcggaggcga cgagcgattt cagaatgacg
    18601 aacaccgcca gcgttgattt caggaatgtc catgtcagtg atttgctcaa agtctgcatc
    18661 ccagatccag ctagtgtcaa ttccatctgc atagttggca ttaaggagga cagataggct
    18721 aaatggataa ggtgctagtt tgatcatttc gatagcttgg gttgcaccga ctggattttt
    18781 aatcaagaca agggtacatt ccttgtcacc gatatgaaag gtttcttggc gtccaaagac
    18841 agcacggctc ttgtcaaatc cctgtttgat gagttgggaa tctgcgccga ggaaacgggc
    18901 gatggccaca gcagctaggg cgttatagat attatagagc ccgccgattt ggataccgta
    18961 ttcttggccg tctatgacaa agcgagagcg attgttggtc aactcaacca gttttgtcaa
    19021 acgatagtcg agatcaggac gtttacatcc acaaccttca cagatatagg cacccaagtt
    19081 tgcataggta ttatgctcat atttgaggat gccttggcag tcaggacaga gaatcccttc
    19141 ggtattgtag tgagcaagtt gggctggtcc tttttccaag tcaaaaccaa aatactctat
    19201 agggtttgga atagttggct tgtagaaaag tggactgtct ccgttaagga gaacagtagc
    19261 agttggaact tttcgaatgg catccaatat catgttatag gtagtataga tttcaccgaa
    19321 acggtccatc tggtcacgga agatattagt aatgacaaaa agactaggct ggatatagtc
    19381 acagatacga gatagactgg cttcgtcaat ttcgaggacg gcaatatttt tcccagtttt
    19441 tgaagatttg gctgttagga aggttgttgc aatccctgta atcatgttgg caccgcttgg
    19501 gttggttaga acttgaccat aaacctcttt taaaatgccg acagtgaggg cagttgtcag
    19561 ggtttttcca tttgttccag tgacaacgac aatctcgtag ttcttagcta ggctttgtaa
    19621 aatatcttta tcaaattgaa gggcgacttt ccctgggagc gtacttccac gtccaagacg
    19681 gcttaaaacg aagtgggaag aacgcccagc aagaaggccc aaagtagttt ttaagttcat
    19741 gtttctatta tatcacaaaa gagggaaaag acagatttgg tgtttcgtag aagtaaaaac
    19801 agcccaaaga gggctgtcag ttaagatgag atttcaactt aaatcgcaaa caagtcattc
    19861 aagttctcag ccaaggttgg gtgagtgaag atttgttttg tgaagtaagt gtaaggaatc
    19921 ttgttgtcca tagcaacagt aatgatgttg atgatttctt gagaaccttc tgagaagatg
    19981 cttgcaccaa gaatttcttt tgtttcagta ttaacaacag ctttgaaagc tccgcgaagg
    20041 tctccattta cgtgaccacg aggcatggct gcaacaggga tttctttcac tgcgtatgga
    20101 agtttcaaat cagctgcttg gctttcagtc aaaccaactt gtgaaagtgc aggtgtgatg
    20161 aacatagtat ttggcacatt gagacggtcc tcaagtgtgt agctgccatc tccagcaagg
    20221 tagctgtaaa caacacggaa gtcatcaagt gaaatgtaag taaattgaag gccaccgttg
    20281 acatctccaa ctgcaaagac accaggaacg tttgtttgac agtgtttgtc tactttaata
    20341 gcaccacgtt cagttagttc aatatctgta ttttcaagtt gaagtggttc tacatttggt
    20401 ttgcgtccag ttgcgtagag aagggcgtcg aaacggtaag tttcgtcttc agttacgaca
    20461 agcacttggt caccatcgtt tttgatttca gtagtatgga tattttgaag caattcaatg
    20521 ccatcttctt ccatgtattg tttagcaaga gctgcgatgg aaggttctgc acgaggtagg
    20581 aatgtatcca aggcatctag gactgtgacc ttgcttccaa gtttgttgta aaggccggca
    20641 aattcaagac cgatatttcc gccaccaagg attccaagtt tttcaggtaa tttgtccaag
    20701 ctttggatac ctgttgagtc aaagatgttt ttgcttgtag caagtccagg aattggcaag
    20761 acgtttgaaa cagcaccagt gttgatgacg attgtttcag cagtcagttc tttcttttca
    20821 tcaccagctt ggatttcgat gactttattt gaaaggaagt gagcttccgc atcaaagata
    20881 tctacgcctg taccagcaac agtcgcatag tttttaccgt tgaggcgacc agtgatcgtg
    20941 tttttagtag caatcacttc ttcaaaagac aagtcctttt cagcagcaac tagcaaggtt
    21001 ttagttggga tacaaccaat gttgatacaa gttccaccgt acatagcctt gctgcgttca
    21061 acgagggcaa cttttttgcc agctgaagcc aatttacctg ctagtgtttt accagcttta
    21121 ccaaatccaa taacaattaa atcgtatgtt aacatttatc ttcctcctat tcgaatttca
    21181 ttctagcaca agaaaaaaac ttttgcacaa ttctgctacg gaaaagacag aaaaagataa
    21241 aaacgctttc cagacaaaaa actagtaatt ttttgacaag cttgataaac taaaatccta
    21301 tctattttta tataagaaag aataaatgtt cgaaaatagg aggttttatg aaatcgaatc
    21361 gttatattat tgcctttgct ggggtcattt tacacttaat gctgggttcg acttatgctt
    21421 ggagtgttta tcgtaaccct attattgaaa aaacgggatg ggatcaggct tctgttgcct
    21481 tcgcctttag tctagcaatc ttttgtttgg gcttatcggc tgcatttatg gggcgtttgg
    21541 tagaaaaatt tggtccgaaa gtcatgggga gtctatctgc ttttctatac gcaggtggaa
    21601 atatcttaac aggatttgca atagaccgtc aggaactgtg gttgttgtat ctagcttatg
    21661 gcattttagg tgggcttggt ttgggagcag gctatattac ccctgtgtca acgattataa
    21721 aatggtttcc tgataaacgt ggtctcgcaa caggtttagc gattatgggg tttggttttg
    21781 cttctttatt gactagtccc atagcgcaac acctcatcgc aggggtaggg attgtagaaa
    21841 ctttttatat tttaggagca agttacttta ttatcatgct cctagcttca caattcatta
    21901 agcgtccaaa tgagcaagag cttgcaattt tatcttcttc agggaaagaa aaaacagcct
    21961 ctttgacgca aggaatggct gcaaatcagg ctctaaaaag caatcggttt tatatgcttt
    22021 ggattatttt ctttatcaac atagcttgtg gtttaggctt aatttcagcg gcatcgccaa
    22081 tggcacagga gatggctggc ttgtctacaa gtcatgcagc agtaatggtg ggtgttttgg
    22141 ggattttcaa tggatttggt cgcttgctct gggcgagttt gtctgactat atcggtcgcc
    22201 ctctaacctt tagtatatta ctgcttgtca atcttttctt ttctctctca ctttggctct
    22261 ttacagattc cgttttattt gtagttgcta tgtctatttt gatgacttgc tatggagctg
    22321 gtttttcttt gattccagct tatctcagtg atatttttgg aaccaaggaa ttggccgctc
    22381 tgcatggtta tattttaaca gcttgggcaa tggctggttt agcgggacct attttattag
    22441 cagagactta taaaatggct cattcgtaca cacaaacctt gttcgttttt ctcattttat
    22501 acagtatcgc cttggctttg tcttattatc taggtcgttc aatcaaaaaa gaaagtcaaa
    22561 aaccgcttac atgattttct gtagaaatgg ggctatcttt tgctctggtc ttgtgttata
    22621 ctagataggt tgcaaagaaa acagtacttt tcttttgtgg aaaagaagca acacgatttt
    22681 atatctagtt atatgaaaat acgtcataaa aagaaaagta taaactaagc cctatagggt
    22741 ggcttcgcac cacctttaga aagaagaata acgtgaaatt taatgaatta aacttgtctg
    22801 ctgatttgct agcagaaatt gaaaaagctg gttttgtaga agctagtcct atccaagaac
    22861 aaactattcc tttggccctt gaaggcaagg atgttatcgg tcaagctcag actggtacag
    22921 gaaaaactgc agcctttggc ttgcctaccc ttgaaaaaat ccgtatagaa gaagcgacta
    22981 ttcaagcctt ggtcatcgct ccaactcgtg aactagctgt ccaaagtcaa gaagaactct
    23041 tccgctttgg tcgtagtaag ggagtcaaag tccgttcagt atatggcgga tcaagcattg
    23101 aaaaacaaat taaggctctt aaatctggtg cccatatcgt ggtgggaact ccaggtcgcc
    23161 tcttggactt gattaaacgc aaggccttga aattacaaga cattgaaacc cttatccttg
    23221 acgaagcgga tgaaatgctt aacatgggct tccttgaaga catcgaagcc attatttccc
    23281 gtgtacctga gaaccgtcaa actttgcttt tctcagcaac tatgccagat gccatcaaac
    23341 gtatcggtgt tcagtttatg aaagcccctg aacatgtcaa gattgcggct aaggaattga
    23401 caacagaatt ggttgaccag tactatatcc gtgttaagga acaagaaaaa tttgacacca
    23461 tgactcgtct catggatgtg gcacaaccag aactcgctat tgtatttggt cgtaccaaac
    23521 gccgtgtgga tgaattgact cgtggtttga aaattcgtgg cttccgtgca gaaggaattc
    23581 atggcgacct agaccaaaac aaacgtcttc gtgtccttcg tgactttaaa aatggcaatc
    23641 ttgatgtttt ggttgcgaca gacgttgcag cgcgtggttt ggatatttca ggtgtgaccc
    23701 atgtctacaa ctacgatatt ccacaagatc ctgagagtta tgttcaccgt atcggtcgta
    23761 caggtcgtgc tggtaagtca ggtcaatcta ttacttttgt tgctccaaac gaaatgggtt
    23821 accttcaaat cattgaaaac ttgactaaga aacgcatgaa aggtctcaaa cctgcaagtg
    23881 tagaagaatc cttccaatca aaaaaacagg tagctctcaa gaaaatcgaa cgtgattttg
    23941 cagatgaaac cattcgtgcc aactttgaga aatttggtaa ggatgctcgc aaattggctg
    24001 ctgagtttac tccagaagaa ttggcaatgt atatcttgag tctgacagtc caagacccag
    24061 atagccttcc agaagtggag attgcacgtg aaaaaccact accgtttaaa ccatcaggta
    24121 atggtttcgg tggtaaagct aagggaggtc gtggaggccg tcgtggggac gaccgtcgag
    24181 agcgtgatcg tcgtggcaat ggtcgccgtg atgagttcaa aaaaggaagt cgtggcaacg
    24241 atcgttttga taaggaaaaa cgttaccgta aggataataa aaaaccacgc aatactttaa
    24301 gcgaaaagca aacaggcttt gttattcgta acaaaggtga taaataagaa aaagcggttc
    24361 aaaatgaatc gcttttttat ataaggagac cgaatgtgaa aaagacaaat aa
//
LOCUS       DAVLFB010000023        23837 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLFB010000023 DAVLFB010000000
VERSION     DAVLFB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.023"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(91..240)
                     /locus_tag="VXD58_000806"
                     /pseudo
     CDS             complement(91..240)
                     /locus_tag="VXD58_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(394..2025)
                     /gene="rlmD"
                     /locus_tag="VXD58_000807"
     CDS             complement(394..2025)
                     /gene="rlmD"
                     /locus_tag="VXD58_000807"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0675544.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLDMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     QEHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2099..3073)
                     /locus_tag="VXD58_000808"
     CDS             complement(2099..3073)
                     /locus_tag="VXD58_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV0675545.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3147..3761)
                     /gene="pmp23"
                     /locus_tag="VXD58_000809"
     CDS             complement(3147..3761)
                     /gene="pmp23"
                     /locus_tag="VXD58_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV0675546.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(3761..4735)
                     /locus_tag="VXD58_000810"
     CDS             complement(3761..4735)
                     /locus_tag="VXD58_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV0675547.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4743..5999)
                     /locus_tag="VXD58_000811"
     CDS             complement(4743..5999)
                     /locus_tag="VXD58_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV0675548.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6061..6492)
                     /locus_tag="VXD58_000812"
     CDS             complement(6061..6492)
                     /locus_tag="VXD58_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675549.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6494..7096)
                     /locus_tag="VXD58_000813"
     CDS             complement(6494..7096)
                     /locus_tag="VXD58_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV0675550.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7080..7919)
                     /gene="prmC"
                     /locus_tag="VXD58_000814"
     CDS             complement(7080..7919)
                     /gene="prmC"
                     /locus_tag="VXD58_000814"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV0675551.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7919..8998)
                     /gene="prfA"
                     /locus_tag="VXD58_000815"
     CDS             complement(7919..8998)
                     /gene="prfA"
                     /locus_tag="VXD58_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV0675552.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8995..9585)
                     /locus_tag="VXD58_000816"
     CDS             complement(8995..9585)
                     /locus_tag="VXD58_000816"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV0675553.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9708..9890
                     /locus_tag="VXD58_000817"
     CDS             9708..9890
                     /locus_tag="VXD58_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV0675554.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10026..11399
                     /gene="mnmE"
                     /locus_tag="VXD58_000818"
     CDS             10026..11399
                     /gene="mnmE"
                     /locus_tag="VXD58_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV0675555.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11446..12381)
                     /gene="dapA"
                     /locus_tag="VXD58_000819"
     CDS             complement(11446..12381)
                     /gene="dapA"
                     /locus_tag="VXD58_000819"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV0675556.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12437..13513)
                     /locus_tag="VXD58_000820"
     CDS             complement(12437..13513)
                     /locus_tag="VXD58_000820"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV0675557.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14144..14216)
                     /locus_tag="VXD58_000821"
     tRNA            complement(14144..14216)
                     /locus_tag="VXD58_000821"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14181..14183),aa:Thr,seq:ggt)
     gene            complement(14316..14825)
                     /locus_tag="VXD58_000822"
     CDS             complement(14316..14825)
                     /locus_tag="VXD58_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV0675558.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14823..14919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14948..15379
                     /locus_tag="VXD58_000823"
     CDS             14948..15379
                     /locus_tag="VXD58_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV0675559.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15461..15838
                     /gene="mscL"
                     /locus_tag="VXD58_000824"
     CDS             15461..15838
                     /gene="mscL"
                     /locus_tag="VXD58_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV0675560.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15880..16974)
                     /gene="hemH"
                     /locus_tag="VXD58_000825"
     CDS             complement(15880..16974)
                     /gene="hemH"
                     /locus_tag="VXD58_000825"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV0675561.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17129..18352
                     /gene="pepT"
                     /locus_tag="VXD58_000826"
     CDS             17129..18352
                     /gene="pepT"
                     /locus_tag="VXD58_000826"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV0675562.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18388..19017)
                     /locus_tag="VXD58_000827"
     CDS             complement(18388..19017)
                     /locus_tag="VXD58_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675563.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            18982..19203
                     /locus_tag="VXD58_000828"
     CDS             18982..19203
                     /locus_tag="VXD58_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675564.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19474..20485)
                     /locus_tag="VXD58_000829"
                     /pseudo
     CDS             complement(19474..20485)
                     /locus_tag="VXD58_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20711..23830)
                     /gene="phtE"
                     /locus_tag="VXD58_000830"
     CDS             complement(20711..23830)
                     /gene="phtE"
                     /locus_tag="VXD58_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV0675565.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         6814 a         4956 c         4409 g         7658 t
ORIGIN      
        1 tattttttga gttttaaact ttggtagaag tcttgaactc cttatattta cataactatt
       61 tgcctctata actgttatga cacgaaacca acaagcaagt tcaagcacct ccatatcttt
      121 attcaaatga ggactaagat attcacaaac tttatcataa acctctttat cttttttcat
      181 aaaaggggca tacaacttag caaatttatc ctaaaatttt ttgttgcctt tatcaatcat
      241 ttcaacgctc ctttaaatat aaaaatgatc ctatcaatct ttatattgag cctattttct
      301 aattgatacc ttgcaagaaa ggaataatag ctgttcctat accaagtagg aatgtaataa
      361 atgcagaaag tcatatactg tcattctttt ctattaaatc attttgaagt gtctcaaagc
      421 atccatgatg gcttcttctt tttcaggtgt acactgtgga ataatttgtt tatccttttt
      481 agacttgttg taatgttctt gtaattctat tccacatttc ttttttatct gtgcaatata
      541 taatgtcgaa acttttaatt caaatttatt ccaaacatat tctttgattt gagcatatgt
      601 tgctttgctc tccgcacttg tcaaatccat ctcatccagc tcaatttcaa cacttatgtg
      661 cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg acgtgatgcg tttgaggaaa
      721 tagatccacc ggctggactt tcttcaattc atatcccaac tcttggtata gtttaatatc
      781 acgcgccatg gttgcgacat tgcaggagat ataggcgatg cgatcggctc ctgtttgggc
      841 gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga ggatcaacca agataacggt
      901 tggttgaata ccttccttga gccatttctt catggcattt tcagccgtgt cacagacata
      961 gtgggcatta gtaatcttgt tcaaagaagc attcttctgg ctattctcta ctgcttctgg
     1021 aatcagttca acaccgtaga cttctttgac atgcttggcg actgataaac caatggttcc
     1081 aataccagaa taggcatcaa taatcacatc atcttttttt aactctgcaa agtcaatggc
     1141 tgtttgatag agtttctccg ccatttcagt attgacttgg taaaaggctg ggccagcgat
     1201 ttggaagtca tttcccaaca tctggtccgt aatatagtct tgaccataaa gagtgcgcca
     1261 ctccttacca aaaatcgcat tggtattctg gtcgttgata ttttgcatga cagacacaat
     1321 ctctgggaac tgcttgataa cttgttcaat caattggtca acacgaaaaa cttttggacg
     1381 agttgtcacc aaaacgacca tgatttgtcc tgaatagtga ccacgacgca ccacaagatt
     1441 ccgaatcaat ccagattgtt ccttttcgtc ataaggtttt aaatcaaaac gacggagcag
     1501 gtctcgaaga gctactacga cttggtcaat gacaggatcc tggataaaga aatcttcaag
     1561 gggcatgagg ttatgcgaat tcttacggaa aaatcctgtt tccaagacac cattcactcg
     1621 acgaacgggc acctgcgcct tattgcgata cttgactgga tgttccatat caagcgtttc
     1681 agcaacttct acatctgcaa ttccagcaat cttgtagaga ctgtccttga cttgcttggt
     1741 tttaaacttg agctgttctg gataagaaag gtgtcctaaa tccgcgattc ctgaacgcag
     1801 gtaagccaaa tctagatctt gattacggtg tggtgactgg acaaggtatt tttcaacttt
     1861 tccaaagcca atctttttat tgaccttgag gacacgcatg agaatttttt cactcggtaa
     1921 agcattctct acaaaaaaga ccaaaccatc taccttggca actcctgccc cttcatgggt
     1981 caaatcaaca atttcaactt ctacaatatc atttttcttt aacattcttt tctctttcta
     2041 ttggccgaca aaaaagacgg caacgttacg gttaccatcc attataccat gaaatttctt
     2101 aagaaccaaa actcttgaag aagttgacaa gggcttgcca gagggagctt aagaagttac
     2161 caccgtcctc tagcgcctta tccgcatcaa agttaaggtt gatattttta aaactgtcgc
     2221 cagcttgtga tacgatgctt tgtttaaggt catttagggt tttagtgaaa tctgcattgc
     2281 tgaggatatc actctttgag agattcaagg caaaattgat gatgatattg atctggtttc
     2341 ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc ttcaacaatc ttgcgtacat
     2401 cttcttctgt cagatttccc ttgctttctt tagctttggc gagtcctgac ttgatatcag
     2461 ctagggcaac gtttaattta ttagcatcat agcctgattt gtccttgttt tcagcattga
     2521 tatctgacaa agcctttagc tcttcttgag ccaaatcttt attggcttgc ggcaccttgg
     2581 ctccattagc ttctagcgaa tagtagatcc cagccaaagc actctcacct gtaactggaa
     2641 taggggctgc tacagtgatt ttggcatgtt ccatacccag cgttactgct gcgtttcggt
     2701 acatatcctg agtcacctta gtgatatttt ctggtgtttc aatcttgacc tcaagtggcg
     2761 atttgtcacc tagcttttga atcttggctg atgaatacaa ctgtaagcta gagtcattgg
     2821 ccacattcat gattttagaa taaacatcag gtgtcatggt cttgagttct ttggtatctg
     2881 ttgaggcatt gtagcccagt tttttaagag tttgattttt ttggtcttca gatagggagg
     2941 aacctaggac atattcaggt tggacatagg tttcatcgat aactttttga acatctgttg
     3001 ctgcatggac gctattcata gctgttactg cccacaagac cgcagcacta gtcagaaaga
     3061 gtttctttct catagggaat ttcctccttt acctttctag agtaatatat ctatcttaaa
     3121 gaaaacttat aacaaaaaca cctgaactag ccagatgttg aaaagagagt gaaacatttg
     3181 atgatgtaaa ggttgagttg cacctgtcta gaataataat agtttcctcc atttacatag
     3241 agttcggcac cgtgaaaaat ggaaatggga tgaatataac tataagtctt tccagtcgta
     3301 ttaccaagca agggggcaac agtctcacga gagtactgtt tggctagagc caaggtattt
     3361 tccttgccat tttgggcgat aaaatcgata taggcaggtc caaaattata ggcttgaaca
     3421 gctgtccaga tatctacacc cttcttctgc gccagataga gattgcctgt cagagtttga
     3481 atgccttgcc gaatgctaga ggcattatca ttgatggtgt tggtggaacc acttgcagac
     3541 tcactagact gcataacatc gccttctttt ccttttgttt cagtataaat catagcaagc
     3601 acaagctctt cgtttgctgg ggtgtctttt tcactcaata tttctcgcac catgggttga
     3661 taggtcatga cttgtttgac atcttgatga acgcggtaag ctttatagcc agcaaaaagg
     3721 aagactgcta gtacaagcac tcttcgaatt cgtttaaaca ttatttactt tggatatcct
     3781 cgatattttt gattaagata gagtaggttc cattttcgtt ttggataaac tcaacagact
     3841 cggcgtcttg atagacgtta ttgggaacga tgagctcaat tccatttgat aaggagagtt
     3901 tttggttttc aaatttcttt aattggcgac tgacatcaat ttcatcaaat tgaacaggtt
     3961 ctggtacggc ttctttgact tggtcaataa agctcaaacg agccgtcaga ttgttgtcaa
     4021 aaaggtcatt agccaatttc tcaggtgaca attcattgct ttcttctagg ttgttgaaaa
     4081 tagctgattt gaccttggat tgaaattgaa aatcatctgt gttaaaagat tcagcaattc
     4141 tctgggctgt tttttccagt tccttgatag attttttagg agaaatctta ggagcgacag
     4201 caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt gtacttgatt cgtttttcaa
     4261 tcaggtgata cttgctactc tgaagattga ccaccaaggc ctcatcagct cctgttccaa
     4321 atccaggcag gttattctga gttagcttga ttggattatc aacttctcct ccgaggtggg
     4381 tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg ttctacacct tctttagaaa
     4441 attgaacaaa aatcaagtca ttggtcttga gattttcaga aatgctaaac tcctctttcc
     4501 agagattagc cagcgttact gatgtctcca acaaatcgtc tgtaatatga ttgaagaagg
     4561 gattttcttc ttcgaaaatc ccagtcttgg cttcatctga atacacatgt tcaatttttt
     4621 tacgcaggta ttcttcgatt tttggagtaa tattgagaaa cttatctgct aagaacagtt
     4681 cggtatcatc cggactgaac tggtgaataa tggctttctt aatataaatg tccataaaag
     4741 ttttagtcct cgtatagtag gaaggcatct gtcaattctt tgactgcact tctcacttct
     4801 tctaatacag cctcattttc tgaattctta agggttttaa tgatgagttc agccactttg
     4861 cgactttctt cttcaccaaa tccacgtgca gtaatggctg ctgctccgat acgaatccca
     4921 cttgtcttga atggtgacaa gctttcgtaa gggattgagt ttttatttaa ggtaatattg
     4981 acttcatcca acaagttttg agcaactttg ccgttttcta caactttagt cacatcaaca
     5041 aggaagagat ggttttcagt tccacctgaa ataatacgga aatcagggtc ttgcaagaag
     5101 acatctgcca tagccttgct gttcttaatt acattggcag catattcctt gaaggctgga
     5161 tccaaaactt ctttgaagga aactgcctta gccgccacaa catgctctaa aggaccgccc
     5221 tgaatacctg ggaaaatagc tgaattgatt tttttagcaa gttcttcgtc attggtcaaa
     5281 atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg ttgttgttgt gatatgagcg
     5341 tatggaactg ggcttggatg aaggccagcc gcaaccaaac cagcgatatg ggccatgtcc
     5401 accatgagct tcgcaccgac agcatctgcg atttcacgga attttgaaaa atcgataatt
     5461 tgagaatatg ctgaagcacc agctacaatc agttttggtt ttacttcttg ggcttgtttc
     5521 aagatagcat caaagtctaa gagttccgtt tcaggatcaa cactataaga aacaaagttg
     5581 taggtttgac cagagaagct aacaggagcc ccatgagtca aatgaccacc tgatgccaaa
     5641 tccattccca taaccgtatc acctggctca atcaaggaca tgtaagccgc acagttagct
     5701 tggcttcctg aatgtggttg gacattggca aatttagcac cgaaaatttc ttttgcgcgt
     5761 tcaatagcaa gagtctctac aacgtctact acatcagttc caccataata acggcgtcct
     5821 gggtaaccct cggcatattt atttgtcaag atagaccctt gagctgccat aacagccttg
     5881 gaaactacgt tttccgaagc aattaactcg atattatttt gttggcgttc ttcttctttg
     5941 gcaatagcat tccagagatc agcatcatat gctttaaaat catctttgtc aaaaatcata
     6001 ggtcttctcc tttattgtgt gactagtcca ttagtttgat tttacaataa gaaaatcaaa
     6061 ctaaaagatg cgaataaacc gtttctgcat tttatcacaa gtatagccaa ctttttcata
     6121 aaatgcatga gcacccagac gatgattggc agaatttaag cggataaacc cataaccaca
     6181 tctttttgct tcttcttcca acccttgtag taaactttta ccaatacctt gaccttgcgc
     6241 ttgaggtgaa actgctaaag ctaagatatt aaatcctgct ttggaataga gtgattcgta
     6301 aacttcagcg tggacatatc caagtaagac atgattagct gcatcctcat agccaagtag
     6361 gaaatgatgg gaatcctgag acagtctagc tagttggcta gccgtttcct ctggactaaa
     6421 agtataaccc aaagcctctt ggttgatgtc acatatcgct ttcacatctg tttcttgcaa
     6481 atctcttagc atttcaagcc tcctcaaaag aaatctctgg caaccgagca agaatatctt
     6541 ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc tccagacaaa tccacaatag
     6601 ttgaatcctg tccagttaga aaagcatcgt cttccagacc cagaacctct tggtcaaaat
     6661 ccttcagaat ttgttcaaag gttacaccac ttgcctgacc tgagatattg gcagacggcc
     6721 caatcaaggg acctgtctct cgaattaaat ccagtgtgat agggtgactg ggcatccgaa
     6781 atccaatagt tgcaaggtca gaatttaccc aatagggaac tcggtcattg gcttcgagaa
     6841 taatggtcaa gggacctggc aaaaaggtct ctacaagttt ttgtagataa gttggctgat
     6901 tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt gagattgagt gccttatctc
     6961 taggacgacg tttgagttgg taaacatggt caactgcttt ttcatctaag gccttggcaa
     7021 aaagaccata aacagtctct gtaggtagaa cgacagctcc accattttcc aactcttgtc
     7081 taatcctgtc catcatcaac cacaaccatc ctattttgac caaattggtc tttgagtgtt
     7141 cgaacttttt tttcaggaag atgttttcta aaaagttcgg gaacactttg accttgcttg
     7201 tatccaattt caaggtaaat cttaccacca tccttgagat agtcttttgc atcttccgca
     7261 attctacagt aaatagctag gccatcctcg tctgcaaaga gagctagatg aggctccgaa
     7321 tgcaaaacat tcaaacctac ctctgactca tcttcacgag agatataggg tggattggat
     7381 acaattatat catatttttc agaaatttct gcaaaacaat cagatttttt aaaaaatata
     7441 ttaagatttt gatcgctagc attctctgat gcaagctcta aggcctcttg agaaacatct
     7501 gctgctgtca ctgaccaatc tggtctattt tttgctagag cgagagcaat agctccacta
     7561 cctgttccaa tatccagaac tgagagattc gtctcaagat tttcagttag aataagctcc
     7621 actaactcct ctgtttctgg acgaggaatc aaaacccgtt catccacctt taaatgcatt
     7681 ccataaaaat ctgcctgtcc aatgatgtac tgagctggct tgtgagctgc tagttgctgg
     7741 taaatatctt ctacaaattg tttttcttcc tctgttgtca cctcctgctg gagggcaaaa
     7801 ataaagtctg taaaagatag atttttcaga ctacgataga caaaagagag gctttccgct
     7861 tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa ataattgagc taatttcatt
     7921 atttgtttaa ttcttctagt ttttgtgttt ggtcataaag caccaaggca tccacaactt
     7981 cgtccaattt accagacaaa atcgtatcta gtttttggag ggtcaagccg atacggtggt
     8041 ctgtgacacg gttttgtggg aagttataag ttcggatccg ttctgaacgg tcaccagtac
     8101 cgattgtcga cttacgctca gcgtcttgtt catcctgagc aatctgagca aagtggtcag
     8161 cgacgcgtgc acggataatc ttcatagcct tctcgcggtt cttctgctgg gtacgttctt
     8221 cctgcatctc aaccttgata ttggttggca agtgaacgat acgaacggca gtcgcaacct
     8281 tattgacgtt ctgtccacca gcaccagagg cgtgatagat gtcgacacga aggtcttttg
     8341 gatcaatgtc gtattcaacc tcttcaactt ctggcataac aagaactgtc gctgtcgaag
     8401 tatgaacacg gccttggctt tctgtcacag gaacacgttg cacacggtgg gcacctgatt
     8461 catacttaag cttagagtat acagactgac ctgaaaccat agcaaccact tctttaaaac
     8521 caccgacacc attcatagag gcttccatga cttcaaagcg ccaaccttgg gcttccgcat
     8581 acttttggta catagttagc aaatctccag cgaaaagtgc cgcttcgtct ccaccagctg
     8641 ctccacggat ttcaaggatg atattcttgt catcgtttgg atcctttgga aggagcaaaa
     8701 ttttcagttt ttcttcatat tcttcttttt cagccttggc atctttgagt tcttgcttgg
     8761 ccatttcttc caagtccgca tctccgcctg attccttaat catctcttcg gcatcgacga
     8821 tattttgaag gacttgttta tactcacggt aggctattac ggtgtcacga ttggaagctt
     8881 cttcttttga aagctccata aaacgcttgg tgtctgaaac gacatcaggg tcactcagca
     8941 attctcctag ttcttcataa cggtcttcta cagcttgtag ttgatcatag atgttcattt
     9001 ttcttcattc tccttattga tttcaggggc aaaataatgt ttacggcaaa ccgagatata
     9061 ggtttcatta ccaccaatct ggatctgttc tccatcgtaa acgggcagtc catcctgtgt
     9121 tcgcaacacc atggtcgcct ttttcttgca atactgacag atggtcttga tttcgtcaat
     9181 cttgtctgct aaaagcaaga gatatttgga accttcgaac aattcattgc gaaagtcatt
     9241 tttcaagcca aaagccatga cgggtatgtc taactcgtcc acaacacgag ctaggtcata
     9301 aacatggtgg cgtttgagaa actgggcttc atcgaccaaa acacagtaag gtttttctgg
     9361 taggtctcgg atatagccaa agatatccgt tgtttcctca atcgcaaggg cagggcgttt
     9421 catgccaatt cgactcgaca catagccaac accgtcacgc gtatccagag ccgaggtcat
     9481 aatcacaaca ccttttcctt gctcctcgta gttataggcc actttgagaa tctcaatcgt
     9541 tttaccagag ttcatggtcc cataacgata gtacaactgt gccatgtttc ttgcttcacg
     9601 tccatttcta aatttttgct acattctagt atatcataat tttcttaagc tttaaacggc
     9661 aaaatgtggt aaaatggaag aaatcaaaac tagtggagga agctattatg ccatttgtac
     9721 gcatcgattt atttgaagga cgcacgctcg agcaaaagaa agctcttgct aaggaagtaa
     9781 cggaagcggt tgttcgcaac actggagcac ctcaatctgc tgttcatgtc atcatcaacg
     9841 acatgccaga aggaacttat ttcccacaag gggaaatgcg caccaaataa gctagcttaa
     9901 gcagaattgc ttaggctttt tcaatctcca agtagcaccc attgaagaaa tagtctaaat
     9961 ttgttacaat ttgaaaagag acttggaaaa ttccaagaaa agagcaatta attaaaggaa
    10021 acattatgat tacacgtgaa tttgatacca tcgctgctat ctctactcca ctaggtgaag
    10081 gggctattgg tattgtccgc ctgagcggaa cagacagttt tgctattgtg caaaagattt
    10141 ttaaaggaaa agacttgaac aaggttgcca gccacactct caactacggt cacattattg
    10201 atcctctgac tggtaaagtc atggacgagg ttatggttgg ggctatgaag tctccaaaga
    10261 ccttcactcg tgaggatatt atcgagatta acacccacgg tgggattgcg gtgaccaatg
    10321 aaattctcca gctagctatt cgtgaagggg ctcggttggc agaacctggt gaatttacca
    10381 aacgtgcttt tttaaacggt cgcgtagact tgacacaggc agaggctgtg atggatatca
    10441 tccgtgccaa gactgacaag gccatgaaca ttgcggtcaa acaattagac ggctcccttt
    10501 ctgacctcat taacaatacc cgtcaagaaa tcctcaatac acttgcccaa gttgaggtca
    10561 atatcgacta tcctgaatat gatgatgttg aggaagctac tactgccgtt gtccgtgaga
    10621 agactatgga gtttgagcaa ttgctaacca atcttcttag aacagcccgt cgcggcaaaa
    10681 tccttcgtga aggaatttca acagctatca tcggacgtcc caacgttggg aaatccagcc
    10741 ttctcaacaa cctcttgcgt gaggacaagg ctatcgtaac agatatcgct gggacaacac
    10801 gagatgtcat cgaagagtac gtcaacatca atggtgtacc tctcaaattg attgatacag
    10861 ccggtattcg tgaaacagat gacatagtgg aacaaatcgg tgttgagcgt tcgaaaaaag
    10921 ccctcaagga agccgacttg gttctactag tgctaaatgc cagtgaacaa ctgactgcgc
    10981 aagacagaca acttcttgaa attagccaag ataccaatcg cattattcta cttaataaaa
    11041 ccgacctgcc agaaacgatt gaaacttcga aactacctga agacgttatc cgtatttcag
    11101 tccttaaaaa ccaaaacatc gacaagattg aagagcgaat caacaacctc ttctttgaaa
    11161 atgctggctt ggtcgagcaa gatgctactt acttgtcaaa cgcccgtcac atttccctga
    11221 ttgaaaaagc agttgaaagc ctacaagccg ttaatcaagg tcttgagctg gggatgccag
    11281 ttgatttgct tcaagttgac ttgactcgta cttgggaaat cctcggagaa atcactgggg
    11341 atgctgctcc agatgaactc atcacccaac tctttagcca attctgttta ggaaaataag
    11401 aaaaatccat gatccttcat tcggtcatgg attttattgt ctttattagt aatctggtct
    11461 taagacccct gttacagttg ccttagttgc ttcgtagtcg ccatctacga caaccttgat
    11521 aatgcgtttg gcatcttctt ctggtgctgg aacaagaggt agacgagtgg gtccagcttc
    11581 aaatcccata tagttaagaa ttgccttaac tggagcagga cttggataag agaagagagc
    11641 attaacctta ggaatgaatt tacgctgaat tgctgcggct ttcttcatat cgctttctgc
    11701 aatggcagta aacatctcgt gcatttcatc cccatttgta tgagaggcaa cagaaataac
    11761 cccatccgcc ccaaggttca tggcatggaa agcatctcca tcctcacctg tataaatcaa
    11821 gaactcttca ggcttgtgct caatcaagta agccatatta gccaagctag tacattcttt
    11881 gacaccgata atatttggat ggtcagccaa gcgaagcatg gtttctggag tcaattcgac
    11941 aactacacgc cctggaatgt tatagataat aattggtagg tcagaagcat ctgcaatagc
    12001 cttaaagtgc tgatacatcc cttcttgaga aggtttgttg tagtaaggaa caatagcaag
    12061 cccagctgcg aaaccaccaa attccgctac ttctttgaca aactcaatag agtcacgcgt
    12121 atcattagta cctacacccg caatcaaagg aacgcgtcca ttgacaacct tttgtacagc
    12181 cgcaaacaac tccaactcct catcgtgggt caaagttgga ctctcagcag tcgttcctgc
    12241 gagaagaatt ccatccgtat gatgggccaa taaatgctca atcaaggctg gaatagcatc
    12301 aaagttaatg gaaccatcct catggaaggg ggtaataaag gctgtaatga ttttacattc
    12361 ttttaaatct tgataagaca cgaaaacacc tctctatttc aaagaaggag ttcatctgaa
    12421 ctcctaaacg atatgactat tttaattcaa atttcaattc agctgttgga cgaaccaatc
    12481 cacgttcatg aagagtttca gcaatctgaa ctgagttcca agcagcacct ttgagaaggt
    12541 tatctgaaac aacccacatg tgaattcctt tttctgcatc caagtcttta cggatacgac
    12601 caacaaaggt atcacgtgaa ccaactgcat tgatggcttg aggatagatt tgatgaacta
    12661 catcatcttc aagaacagca cctgggaagg atgcgatagc tgctttgact tcttcaattg
    12721 gagccacttc ctttgtttca atataaactg actcagagtg agctgacaag actggaatac
    12781 gcacacatgt cgcagatact gcaatgctat catcttccat aattttctta gtttccttgg
    12841 tcatcttcat ctcttcgtac gtgtaatcat tatcagtgaa gacatcaatt tgtggaagag
    12901 cgttaaaggc gataggataa tgtttcttgt caccacctga aggcaagatt tccgcatgca
    12961 aatcacgtgg tttcactcca tcattcaaga cctcacgaag ttcacgttgt gtctcaagaa
    13021 ttgctcccat accagcacct gaaacggctt gataagttga aacaatgata cggtccaagc
    13081 cccatttttg gcgaaccggc tcaagagcca ccatcatttg gattgttgaa cagttagggc
    13141 aggcaatgat tccgttgtgg gcatcaagtg catgagcatt gacctctgga acaaccaaag
    13201 gaacatctgg attttgacgg aaataagatg tattatccac tactaccacg ccagctttta
    13261 ctgcgtatgg tgcatactta gctgatgtcg aaccacctgc tgaaaagaga gcaatatcaa
    13321 ctccttcaaa agctgtttca gtcgtttctt caatcgtaat atcttgatct ttaaatttca
    13381 atgacttacc tgctgaacgt gcagaagcaa ggtaacggat tttatcgatt ggaagtgttg
    13441 attcttccaa catttttatc atctgagcac cgacagcacc tgtcgcgccg actacagcaa
    13501 ctgtatatcc cataaataac ctctttcgga attttctaaa aatttctata atagaagtat
    13561 tatactactt tttccatgaa ttgcaaagct ttttcatcat tttttggaaa ataaaaatcc
    13621 ccaatcgcta gactagggat taagaggcat cttcatgtga tgagcaggtt cacacaactc
    13681 atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa tagtagcccg aagactacct
    13741 atcgactcat cgcatctact attctactag atagagtcat ttttgtcaac agccaaaact
    13801 ctttatactc ttcgaaaatc tcttcaaact tcgtcagcgt cgccttgccg tatgtatggt
    13861 tactgacttc gtcagtttca tctacaacct caaaaacatg ttttgagctg acttcgtcag
    13921 tttcatctac aacctcaaaa acatgtttta agctgacttc gtcagttcta tctacaacct
    13981 caaagcagtg ctttgagcaa cctgcggcta gtttcctagt ttactctttg attttcattg
    14041 agtattagtt ttatttacac aggtgcataa gaaatatatt ggaaaaattg ggggcagaca
    14101 taacaaaaac ccttgtaaat caagggcttt tctgtctatt taatgctaat tgccgggatt
    14161 gaaccggcga cctcatcctt accatggatg cgctctgcca actgagctaa atcagcttac
    14221 ttaagaagta tactatattc agaaaaactt gtcaagtttc cttagaaatt ttccatagga
    14281 aaaagccagg taagaactac ctagcttatc ttcttctatt tacttaaatc aattcgacga
    14341 ccaattttac caataataat cgctccaata atcaatgaag caaattcacc gattcctgtt
    14401 gtaaaccaag taaggaagaa tggtaattct gctacaatat ttaactctgc agcgatagta
    14461 aacatggaaa ttgaaaagag gattgaaaag aagaaatgat cttttcgaat caatccgtta
    14521 aagaggtaat ccttgctata ttttgcaaaa agccaaacac ctaaactgag gaatactaag
    14581 gttgaaccac caccaacaaa gacatctagc agtccgaagc taaagaaatt agcaatcata
    14641 caaccgatgg taactccgat gatgtactta ggattgtaaa aagccataaa gttcatcatt
    14701 tctgaaatac gaaactgata agcaccatag ctgatggcat ttaggggcgg tgtgacagtc
    14761 aaaacgacgt agatagcagc gacgattgca atatctgcaa catcacgaat agttaatttt
    14821 ttcatctttt ctcctttggc ggttatccgc gtgtaatatg cttggtgaaa gaagctaagc
    14881 accaagggtt gattcattca accttactag tatagcacaa tagcctgctt tatgctatac
    14941 ttaaagcatg aaaaaaccta ttcaaaaatt ttttaacaat gaaatcttag cctatctctt
    15001 ttttggcctt gcgacaactc tagtttcgat tctctcacga ctagtcattt atcaactaag
    15061 tcatcaggaa ctccttgcta cggccttggc aaatattatc ggtatcttat ttgcctttat
    15121 cacaaatgat acgattgtat ttaagcaaaa aaagaaaaat agacttatcc gtttagtgaa
    15181 attttctctt gctcgccttt ctacttttct gttagacttg tttttaactt ttctctttgt
    15241 aacccaattt cctcacatca tagggcaatt cgtaactgaa aacattgata agataaatgc
    15301 tattgaaaca ataattgcac aaatattcat tattatcatc aattatatct tgagcaaggt
    15361 ctatattttt agaaaataat gctccattta tgagcctttt aattgccttc tataatgatt
    15421 taggatacaa tgaaacacga aacattgaaa ggaaaacagt atgttaaaaa atctaaaatc
    15481 gttcttgctt cgaggaaatg ttattgacct tgctgtcggt gttgtcattg cctctgcttt
    15541 tggtgctatc gttacttcac ttgtaaacga cattatcact cctcttattt taaatcccgc
    15601 tttgaaagct gctaaagtcg aacgtatcgc tcaactttct tggcatggag tcggctatgg
    15661 taacttttta agtgctatta tcaattttat ctttgtgggt accgccctct tctttattat
    15721 caagggcatt gaaaaagcac agaaactgac tggcataaag aaagaaaaaa ctgccgaaaa
    15781 aaaaccaacc gaattggaag tccttcaaga aataaaagct ctccttgaga aaaaataatc
    15841 gattaaaagg atctagcgca aagctaaatc cttttttctt tactctttgg gtaacttgcc
    15901 tgctttttca agcatttttt tgataagata aggcatctta acattctccc gccctttttt
    15961 ctcaatcagt tttttcacaa attctggcat ttgtaaatct tcagattcag ctaaaatgtt
    16021 tttcgtctcg tctgaaggaa ctaactcatc aaaggtctct tcttctggga gaaattcctt
    16081 aaactcttga ttttcattga ctctaactgt attgaccaaa gcatcaatca aacgagaatc
    16141 tgtatttggc attggtggac gatggtagtt taccccaaat tcctggcata agtcataaca
    16201 ttctacatca ttgtcaaaca agacctcaat atgctcactg atgaaactaa taggtacaaa
    16261 aatataatgg tctggatgtt ctgtctgttc tctgagatac tccaaaacat ctggtttaat
    16321 ccatggaata ccaatatcac tctcactctg ccaagtgttg gtatattgtt ctgaactcaa
    16381 gcccagtttt tcagcgacta gcttgctatt ttcgaaaatt tggtctatat aaggatcacc
    16441 aaagtccaag gcaaaaatgg gcacactatg ggctgaaaag atgactttaa agctgtcttg
    16501 ctttacttct tcttttaaaa tcttagcaat ttcatctgcc caatagttta agagcgcttc
    16561 ttcctgatac cagtccttaa tgaccaaaaa ttgaatttgt ttgctttcta aaaatttttc
    16621 atagcccatg acagagtaga aagaataatg gggctccaaa atcaagcaaa tacactgttc
    16681 aattccgtct gcttccattt gaccgatcac atctggaata aagggactgg aaaacttatt
    16741 agcaaagtag acactgtatt cattccccag cctagcttct accaaggcaa cctcttgacg
    16801 ggtaattttt tgcagaggcg tgcctccaat tcgtacataa ttatcataga gagtctgaat
    16861 ctcgtggtct tggggtctca ccccacgacg aatatttgtg aaaaaatcag ctacaccttc
    16921 aaaggtaatc tcttctggtg aaccgaaggt catcattaaa attgcttttt tcatgactgc
    16981 ttccttgtga tgtttttatc aagtttattt tatcattaat taatcgataa gtaaacgcta
    17041 atatagggac attctcaatc ttctgtcttt tgtttttctc ttctttctat gatacaatgg
    17101 aaaaaataaa ttcaaaagga gtttttttat gacttatcca aatctcttgg accgcttctt
    17161 aacctacgtt aaggtcaaca cgcgctctga tgaacactct actactactc caagtacaca
    17221 gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa atgaaacgtg ttggactgca
    17281 aaatgtttac tatctaccga atggttttgc tattggaacc ttgccagcca acgatccgtc
    17341 tttaacacgt aagattggtt ttatatcgca catggatact gctgatttta atgctgaagg
    17401 agtcaatcca caggtaattg aaaactacga tggtggtgtg attgaactag ggaattctgg
    17461 tttcaaactc gatccagctg acttcaagag tcttgaaaaa tatccaggac aaacgctcat
    17521 cacaacagat ggaacaagct tgctaggtgc tgatgacaag tcaggaattg ctgaaattat
    17581 gacagccatt gaatatctaa ctgctcatcc tgaaattaag cactgtgaga ttcgtgttgg
    17641 ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa tttgatgcag aagattttga
    17701 tgtggatttt gcctacactg ttgatggtgg tccactaggt gaacttcagt acgagacttt
    17761 ctcagccgct ggtgctgaat tgcatttcca aggccgtaat gtccaccctg gtactgccaa
    17821 agagcagatg gtcaatgccc ttcagctagc aattgatttt cataatcaac ttccagaaaa
    17881 tgaccgacct gagttaactg aaggttacca aggtttttac catctaatgg atgtgacagg
    17941 tagtgttgag gaggtgcgtg caagctacat cattcgtgat tttgaaaaag atgcctttga
    18001 agcgcgtaaa gcatccatgc aatctatcgc tgataagatg aatgcagaac ttgggagcta
    18061 ccgtgtcact ctcaacttga cagaccagta ctacaatatg aaagaagtca ttgaaaaaga
    18121 tatgactcca attaccattg ctaaagccgt tatggaagat ctaggtatca cgcctattat
    18181 cgaaccaatc cggggtggaa cagacggctc taagatttcc tttatgggaa tcccaactcc
    18241 gaatatcttt gcaggtggcg aaaatatgca cggacgtttt gaatacgtta gccttcagac
    18301 tatggaacgt gcagttgata ccatcattgg catcgtagct tataaaggct aaaaagacga
    18361 ggtagctcag ctacttcgcc tttcttttta ttctactggt ttttcttgat ttccagtagt
    18421 tgtagaagat tctgttgttt cattttctga agttgattca gcaggtttag aatctcttgt
    18481 attgcttggt ttgttttcgt cgctagcaat ttcaatgtta gattctgcag ttgcgtttgg
    18541 ttggttctca gcactggtgt tatcactatt tgcttcagca tttcttgctg gacttgtttc
    18601 ttcacttgcg ctagcttttg actggatttg atgattcaaa actagaatag cttttgtcga
    18661 ttcaagtaaa gctgttttgt ctttactctt agcagaaagt tgatctaata atgcatccac
    18721 cttatcaaag tccgcatcag atccattatt actttctaaa taagagtgaa gcgacatgag
    18781 aatatcgtag agtttttgat agagtacaag tgtctgagaa tcttgctcag cattttcctt
    18841 ttcttgttga agggcgctag cgatacgagt caagacatct tttacctgac tgtttacttc
    18901 atccaagtct gcatcagcct tgtttgtggc agcttttaga ttttctactt cttctgccaa
    18961 ggattgtctg attccttctt catggatttg ttccaagagt tgatttacct tgctcaaaag
    19021 actttctact tcttccttgc tatctgtcgc agattattgg ttgccatcta ccatgtactc
    19081 ctaaaacagg agagttataa tccaagatta caaggcctta cagaaataag aaatccagat
    19141 aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc agcacggatt caatatgctt
    19201 taattttaaa gtttaggtgt caagacctct ttttagtgtg cccaaaattt agagaagtaa
    19261 tcaatcaact aacttttatt tttttcaaac tttcagtaaa ctgacctaaa gctaactcaa
    19321 tctgtctttg tagatgcttc tgctatcagc tagaagttga tctacttttg ccaagactgc
    19381 cttctcatca aaagttccag gttgatagtt ggattgcagg gatggaatct tgtttttcaa
    19441 agccgcttca tatcccttag tttgaacctt gatgtagtga ttgtggtcgc catgaggaat
    19501 cacaaaacct tctgaatctt cacttataat tcgattggca tcaaaaccat gaccatcttc
    19561 ttcctcatga tggacatgta gtgacggatt acttaataca gaactagaag aacttcctac
    19621 ctcttccgtg ttagagtgtg atgggggatt gttaagagat gacttaggaa tatagtgata
    19681 gtgatcccca tgtcttacta tataagcatc acctgtatct ctgacaatat cattagggtt
    19741 aaagacatat gtggctgcta attcacctgc cgacaagtca ctctcaggaa tgaaatgata
    19801 gtgaccacca tgtggtacta tagtagattg aaatagaata tgagcaaatt gataagggga
    19861 ttttaaagta atttctaaca atgatttaga aactatgatg tgctattcta aattcaactc
    19921 actatatata accatcatcg gtagtataac gtccctgtaa ttttgctaca gatacttctg
    19981 cactagctcc tttatcgtct ttaccatgtt cttgtttttg gcgattgatt tcatcttttg
    20041 ttcgtacatt ttctgcatga gcttgatctt taaggtaaac ataatacttt ccatctacct
    20101 taataatata tcctccctta acctaactga cgatatcttg atctttcggc tgatagttgg
    20161 gggctttcat taatagctct tcactaaaga gcgcatcaaa aggaacttta ccattatagt
    20221 agtgataatg atcgccatga gaagttacat aaccttgatc tgtaatctta ataacaattt
    20281 gttttgcttg aattccttct ttttgactaa cctagtctgg agtcaaattt tcagtcttct
    20341 tagtgtcttt attactgttt acatatgaaa cacgattttt atctgtattg gcctgttagc
    20401 tatgttggtt cagagcataa acacacagac ttaaggaaag gataacaaca gatccagctg
    20461 ctatatattt ctttttaaat ttcataatta cctcatttct ataattattt atatgatgtc
    20521 ttcattatta aatgattaaa taaattaatt aaccaattaa ttaactagta aatattccac
    20581 ctctttttaa gttgtatgtc aagaaatttt atatattaat aataaaatga aattctccca
    20641 aagtcagagt tttatttcta acttttgaga gaacttcatt tttgattcag actttttcta
    20701 ctgctattcc ttacgctatg agatcagata aattcttttt tatcacttct ccacttggca
    20761 atcttaattc aatcgttcca tccatattga atataacact atctaagcct aatccgtaac
    20821 tagctgtaaa tttttctaat ttttcttgta caggatctac tgctggagct tcctctaatg
    20881 ctggatctaa catagggtca ctccccacat tcccttctgg attcaacatt ccattatccg
    20941 ttgagttttc tggttttaca ggtttttcgt ttggtgcctc tggtaaagaa tctgctggtt
    21001 tattttctgt tggttggttc tcaactgttc cagtagatac ttttccattt tcagatggtt
    21061 tattttcacc atttccttga ggtgcttctc ctgtaaaatc tgccatattc tttttaatga
    21121 cttctcccga tggtaaatat aattcaattg ttccgtccat attaaacaag acattttcta
    21181 gcttcatccc ataactttca gcaaattttg ctactttttc ttgtacagga tccactgtag
    21241 gaacttcttc taacgttgaa ttactagtac tattcccagt ttcagaaagt ttttcttttt
    21301 ctaccttctc actagtcttt ggttcttcta ccttttcatc aagttttgag ttttcttgtg
    21361 ctttattcct tttaaattgt ggtagaatac ttggtttatc agtttgattt tctttttcca
    21421 agataggtac ttccacaata taagtcgatt gattgtccaa ataagcattt gccatgaagg
    21481 ttacaggaat tttatttccg gccgttctgg ttgttccttg gtttaatttc ggaatcggta
    21541 atttgatttc accaacttta tagttatttt ctaaataagc atttccatga aattcatcaa
    21601 acactctgac taaagcatca gttcctttag gcactgcaaa ttgagggttc actcttaaat
    21661 aagtatcccc tgcatggaaa ggatagaaaa tcgtttgact ggccattttg taagctaaag
    21721 aggttggaac tgtaaatgta ccatcataac ttacttctgg ataatctttt gaagcgatag
    21781 tatacttaaa tgtttgtcct ggtaaataag gttgatctaa ttcaaagttt gcaatattcc
    21841 ctactccttc tccaaatact ttaccagata ctttctccaa tacttttcca tctggtgtta
    21901 ttaattttac tagcatattg atacctaatt ttttctccaa ttcaggcgga aaactaaaag
    21961 aaacgcgttt ttgaccattg gctagagtaa agttttgatt attaaacgta ctatttttta
    22021 acaaattaac aacattcgtt aattcttctc cagtataaac tttattccct tcttttttag
    22081 caactccttc ttcgggttta aacagttcat agttactgtg agaatgacca attccaaccg
    22141 gtttatgttc atcaatcgga tctgcatgat ggtgatctcc atgcggataa ataatcgcat
    22201 ttttttcttt attcacgaca atactttcac gtttgacacc atattgtttc ataatgccag
    22261 caattttttc ttcgattttt ttatctaaat ctttcatttc tttggcatta cttggataat
    22321 cctgttcatg agatgacaaa gaatctaatc cattatgact agttttaact tcctctaaat
    22381 gtttttgcgc agccttaatt tgctcttctg tcaagtcctt cttgaagaaa taatgattgt
    22441 ggtctccgtg actcatgaca aaacctgatt catcttcagc gataatacga ttagcatcaa
    22501 atccgtatcc atcttcttca tgtttctcat gtgaagttcc tggattgatt ggaagagatg
    22561 gagaaggtgt tgctagacta ttgtttggaa gagtcggttg cccaatttga tttgattttg
    22621 gaatgtaatg gaaatgatca ccatgtctta caatataagc tgtagccgtt tcttcaacga
    22681 tatcttttgg attaaaaata taaccatcag atgctgaaga gagctcctta cttgtcgtta
    22741 aagaagaagg attgcttgaa agactgccta gactagacac tacttcatta ggttttgcat
    22801 ttgtagaaac tgtagaacca gttccactga taggcaccat tctggcaatc ttttcttcta
    22861 aggcagaaag cttgctgtaa ggaataaagt ggtaatggtc gccatgcgga atcgcaactc
    22921 catttggtgt acgactgata atcttagcag ggtcaaagac caggccatct gattcactgt
    22981 aacgttgggc gctaggtgaa tcatagagtt ccttcaaaag actctggaga ttttcagatt
    23041 tatttgctgg cttgctagtt gatccttttg ctacagattg cgtgttattg tcactagctg
    23101 ttgaagaata gcttaactga ctcggttgca tattttttcc agccagatgt gctttagctg
    23161 ctgctaattc actagcagat aaatcgcttt tgggaatgta gtgatagtga cctccatgag
    23221 gaacgatata agcattaccc gtatcttcga taatatcagc tggattaaag acataaccat
    23281 catttgtcgt atatcgtccc tgagaccttg ctacagcaac attagagtta accttctcat
    23341 tatctttgac atgttcttgt ttttgacgat tgatttcatc tttagttcga acattatcag
    23401 catgagctgc atctttcagg tagacataat attttccatc gaccttgatg atataaccac
    23461 ccttgacttc attgacaata tcagcgtctt taagttgata gtttggatcc ttcatcaaga
    23521 gttcttcact aaagagggca tcataaggaa ctttcccatt atagtaatga tagtggtcac
    23581 cgtgtgacgt tacatagccc tgatctgtaa ttttgattac aatttgctca gcctgaattc
    23641 cttctttctg gctaacctgg tctggtgtca agttttcact tttctgactt gactggctgc
    23701 catccacata agagacacga ttattgtcct tattttcctg cgaacgatgc tggtttagtg
    23761 cataggcaca tagactcaag gatacgataa cagctgatcc agctgctata tattttttac
    23821 taaatttcat aaatccc
//
LOCUS       DAVLFB010000024        23655 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLFB010000024 DAVLFB010000000
VERSION     DAVLFB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.024"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..1083
                     /locus_tag="VXD58_000831"
                     /pseudo
     CDS             1..1083
                     /locus_tag="VXD58_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1182..2804)
                     /gene="groL"
                     /locus_tag="VXD58_000832"
     CDS             complement(1182..2804)
                     /gene="groL"
                     /locus_tag="VXD58_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV0675566.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2820..3104)
                     /gene="groES"
                     /locus_tag="VXD58_000833"
     CDS             complement(2820..3104)
                     /gene="groES"
                     /locus_tag="VXD58_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV0675567.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3260..3655)
                     /locus_tag="VXD58_000834"
     CDS             complement(3260..3655)
                     /locus_tag="VXD58_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV0675568.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EVDFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3733..4494)
                     /locus_tag="VXD58_000835"
     CDS             complement(3733..4494)
                     /locus_tag="VXD58_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0675569.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4536..5162)
                     /locus_tag="VXD58_000836"
     CDS             complement(4536..5162)
                     /locus_tag="VXD58_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV0675570.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5178..5495)
                     /locus_tag="VXD58_000837"
     CDS             complement(5178..5495)
                     /locus_tag="VXD58_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV0675571.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5492..5791)
                     /locus_tag="VXD58_000838"
     CDS             complement(5492..5791)
                     /locus_tag="VXD58_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV0675572.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6076..6267)
                     /locus_tag="VXD58_000839"
     CDS             complement(6076..6267)
                     /locus_tag="VXD58_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0675573.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6428..7102)
                     /locus_tag="VXD58_000840"
     CDS             complement(6428..7102)
                     /locus_tag="VXD58_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675574.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(7108..7554)
                     /locus_tag="VXD58_000841"
     CDS             complement(7108..7554)
                     /locus_tag="VXD58_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675575.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7668..8171)
                     /locus_tag="VXD58_000842"
     CDS             complement(7668..8171)
                     /locus_tag="VXD58_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0675576.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8168..8530)
                     /locus_tag="VXD58_000843"
     CDS             complement(8168..8530)
                     /locus_tag="VXD58_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV0675577.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8596..10455)
                     /locus_tag="VXD58_000844"
                     /pseudo
     CDS             complement(8596..10455)
                     /locus_tag="VXD58_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10494..10943)
                     /locus_tag="VXD58_000845"
                     /pseudo
     CDS             complement(10494..10943)
                     /locus_tag="VXD58_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(11067..11783)
                     /locus_tag="VXD58_000846"
     CDS             complement(11067..11783)
                     /locus_tag="VXD58_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0675578.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12664..14079)
                     /gene="ply"
                     /locus_tag="VXD58_000847"
     CDS             complement(12664..14079)
                     /gene="ply"
                     /locus_tag="VXD58_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV0675579.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14090..14422)
                     /locus_tag="VXD58_000848"
     CDS             complement(14090..14422)
                     /locus_tag="VXD58_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675580.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14493..15101)
                     /locus_tag="VXD58_000849"
     CDS             complement(14493..15101)
                     /locus_tag="VXD58_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675581.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15114..15572)
                     /locus_tag="VXD58_000850"
     CDS             complement(15114..15572)
                     /locus_tag="VXD58_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV0675582.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15697..16096
                     /locus_tag="VXD58_000851"
                     /pseudo
     CDS             15697..16096
                     /locus_tag="VXD58_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16861..17121)
                     /locus_tag="VXD58_000852"
     CDS             complement(16861..17121)
                     /locus_tag="VXD58_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675583.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(17180..17731)
                     /locus_tag="VXD58_000853"
     CDS             complement(17180..17731)
                     /locus_tag="VXD58_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675584.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHLKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17721..18716)
                     /locus_tag="VXD58_000854"
     CDS             complement(17721..18716)
                     /locus_tag="VXD58_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675585.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHILKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            complement(19124..19549)
                     /locus_tag="VXD58_000855"
     CDS             complement(19124..19549)
                     /locus_tag="VXD58_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675586.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(19693..20061)
                     /locus_tag="VXD58_000856"
     CDS             complement(19693..20061)
                     /locus_tag="VXD58_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675587.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(20083..20289)
                     /locus_tag="VXD58_000857"
     CDS             complement(20083..20289)
                     /locus_tag="VXD58_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675588.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(20276..20437)
                     /locus_tag="VXD58_000858"
     CDS             complement(20276..20437)
                     /locus_tag="VXD58_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675589.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(20796..21752)
                     /gene="lytA"
                     /locus_tag="VXD58_000859"
     CDS             complement(20796..21752)
                     /gene="lytA"
                     /locus_tag="VXD58_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0675590.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(22128..23498)
                     /locus_tag="VXD58_000860"
     CDS             complement(22128..23498)
                     /locus_tag="VXD58_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0675591.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         7156 a         5246 c         4085 g         7168 t
ORIGIN      
        1 tagaaagcgt cgattcaagt gttatcactg ttcaaaaatg atggtcgctg aaacttctat
       61 cgtcaagaag aatcaccaaa tccctcgtat tatcaaccaa aaaattgcgc aaaagttaat
      121 tgaaaagatt tctatgaccg atattgccca tcagctttcc atctcaactt caactgttat
      181 tcgtaagctc aatgattttc actttaaaca tgatttttct tgtcttcctg agattatgtc
      241 ttgggatgag tatgctttta caaagggaaa gatgagtttc attgcgcaag attttgataa
      301 tcttaatatt atcactgttc ttgaaggcag aacacaagct gtcatccgaa atcactttct
      361 tcgctacgat agagccgtcc gatgtcgcgt caaaatcatt actatggata tgtttagccc
      421 ttactatgac ttggctaaac agcttcgctt tcaaatttct aggctcaggc tgaaacagtc
      481 tcccagactg tttcactccc gaatgctaaa atcgttcttg atcgctttca cattgtacaa
      541 catcttagcc gtgctatgag tcgtgtgcat gtccaaatca tgaatcagtt tcatcgaaaa
      601 tcccatgaat acaaggctat caagcgctac tggaaactca ttcaacagga tagccgtaaa
      661 ctgagtgata agcgatttta tcgtcctact tttcgtatgc acttaaccaa taaagagatt
      721 ttaaacaagc ttttgagcta ttcggaagac ttgaaacacc actatcagct ctatcagctc
      781 ttgctttttc acttccagaa caaggaacca gagaaatttt tcggacttat tgaggacaat
      841 cttaagcagg ttcatcctct ttttcagact gtctttaaaa ccttcctcaa agataaagaa
      901 aagattgtca acgcccttca actacactat tctaatgcca aactggaagc gaccaataat
      961 ctcatcaaac ttatcaagcg caatgccttt ggttttcgga actttgaaaa cttcaaaaaa
     1021 cggattttta tcgccctgaa tatcaaaaaa gaaaggacga aatttgtcct ttctcgagct
     1081 tagctgactt caacccacta cagttgacaa agagcctata aaaagaaggg ttagtcattc
     1141 cctccttttg tgttttttat aagttgtttt ctatagaaag cttacatcat cccgcccatc
     1201 atacttggat ccattgctgg agctggggct actggttctg gtttattggc tacgactgct
     1261 tctgttgtca aaatcaagct ggctacagat gctgcatttt gtagggctga acgactcact
     1321 ttaactggat caatgatacc ttgatcaatc atgttaaccc actcgccagt tgctgcgtta
     1381 aatcctatac caagctcagc atttttcaaa cgatcgataa cgatagatcc ttcaaatcct
     1441 gcattgtgag caatttgacg aacgggttct tccaaagcac ggagaacaat attacgtcct
     1501 gttgcttcat ctcctgtcaa ttccaaggta gcaacagctg gaatcacatt ggcaagagct
     1561 gttccaccgc ctgcaacaat accttcttca acagctgcac gagtagcgtt gagggcatct
     1621 tcaatgcgga gtttcatttc tttcaactca gtttcagttg cggctccgac cttaataacc
     1681 gctacaccac ctgacaattt ggccaagcgt tcttgcaatt tttcacggtc aaattcagaa
     1741 gttgtagttt cgatttgaga cttgataacc gcaacacggt gagaaatcgc ttcaggattt
     1801 cctgcacctt ctacaataac cgtgctatct ttgtccacgg tcactctcgc tgcttgacca
     1861 agagcttcaa ttgtcgcatc tttcaactca agaccaaggt cttctgtgat aactgttccg
     1921 cctgttaaga tggcgatatc ttcaagcatg gctttgcgac ggtcaccaaa accaggtgcc
     1981 ttgactgcta ctacgttgaa ggttccacga atcttgttca aaacaagagt tggaagagcc
     2041 tcgccatcca catcatccgc aataatcaag agtggacgat tgctttggag aatgctttcc
     2101 aaaagtggca agatctcttg gatattggaa attttcttgt ctgtaatcaa aatgtacgga
     2161 ttttcaaggt cagccaccat tttttcgcta tctgtcacca tgtactgtga aaggtaacca
     2221 cggtcaaact gcattccttc tacgacttca agctctgttt ccataccacg tgactcttcg
     2281 atggtgatga caccgtcttt gccaactttt tccattgctt cagagatgta ctcaccaact
     2341 ttttcagaac gagaagatac ggctgcaact tgagcgatag cttctttatt ggcaacaggg
     2401 atggcgttgt ttttcaaagc ttcaactgct gcggcaactg ctgtttcaat cccacgacga
     2461 ataccgattg gatttgcacc tgctgtgacg tttttgattc cttcacggac gatagcttgg
     2521 gtcaagactg ttgcagtcgt agtcccgtca cctgcgatat cattggtttt agaagctact
     2581 tctgatacca atttggcacc catattttca aaatggtctt ctaattcaat ttctttggca
     2641 atagtcacac cgtcattggt aatcaatggt gagccaaatg atttttcaag aacgacattg
     2701 cgaccttttg gtcccaaggt tactttaaca gtatctgcaa ggatatcgac accacggacc
     2761 atagctgaac gggcatctga tgaaaattta atttcttttg acatacttac tttctccttc
     2821 tattcttcaa tgattgccaa gatgttagct tcgcctacga tgatatactt ttcatcgcca
     2881 tctttgacat caagacctgc gtgggcttca actaagacac ggtctccagt tttaacactt
     2941 ggagcaacca agtcaccgtt caaggtacga acaccttgtc cagtagccac aacttgggct
     3001 gttttggttt tttcttgggc tgaacctgcg agaacaaagc ctccaacagt ttgttctttt
     3061 tcttctactt ttaagaccaa acggtcccct aatggtttca acatctgatt tcctccataa
     3121 tgagatagat attattagca ctctttatac ctgagtgcta attcatagtt ctattgtatc
     3181 acttagtcag aaataatcaa gaaaaaagtc tgactttctc aagataaaaa gcctgagacc
     3241 aactcagact ttttaattct taaaatggca attcttcctc ttccaagacc aaatctgcca
     3301 aatcttggcc tgcattattt tcacgcatag cacgttgggc acgactttcc aagagttgga
     3361 atcctgtcac aaggacttcg gttacataat tcatctgacc atttttctca aagcgacggg
     3421 tacgcaattc tccatcaacg gaaatgagac tacctttggt tgcgtagctt gccaaagttt
     3481 ctgctagtct gccccatagg accatattga caaaatcaac ttcacgttca ccgttttggt
     3541 ctttgtaacg acggttgaca gcgatagttg ctcgcgctac tgacttgtca ttgttggttt
     3601 tgtgcaattc tggtgtagac gttaaacgtc caatcaagat aactttatta tacatatttt
     3661 cttcctccta cttatctatt cgtaggaaat caaaaaaagt tacagaaatt tgtaactttt
     3721 cgagaaaatt ttttattttt tatgaaccat gaaacctgtc gcctgttgat tggccataat
     3781 ggtcatatct gtaatctgaa cacgacgagg ttgactagtc acatagacta ctgtatctgc
     3841 aatatcctga gcttgcaaag cttctattcc ttggtaaacg gacgcagctc gttctttatc
     3901 accatgaaaa cgcactgtag aaaaatctgt ttcgacaatt ccaggctgaa tggttgtcac
     3961 cttgatatcc gttgcgatgg tatcaattcg cagtccatcc gaaaaggtct taaccgcagc
     4021 cttggtagct gagtaaacag cggcaccagc ataggcgtag attcctgcgg ttgaccccat
     4081 attgataata tgaccttgat tgacttttat cattgctggc aagaaacagc gagtaaccgc
     4141 catcagacct ttaacattgg tatccaacat ggtcaacata tccaactcct catagtcttg
     4201 atagggagcc aagtcaagag ccagtccggc gttgttgacc aagatatcaa tcctccctat
     4261 cgtttctaga atatcagagc aaacagtctt caccatgacc atatccgtca catctagtgg
     4321 aaaagtccag acggtttgat ttggaaagct tactgcaaac tccgacttga ggatttctaa
     4381 tctgtctatc cgtcgtcctg ttagaacgac atcctcaccc tgctccagat aagcacgcgc
     4441 aatcgcttca ccgattcctg aggttgctcc cgtaatcaca acattttttg ccatcttatt
     4501 tccttctagc tggtctatca gatattaaca acttcttagg cagtccagtg tttagctgag
     4561 tcaaatggtg ttccgacaac ttggtcttct gataattcaa gcaccccacg tttttgtgga
     4621 gcatttggca gatgcaattc acgaggactg cacatcatac caaaactctt ttcaccacga
     4681 agttcacctg ggaaaatgag attccctttt ggcatcatag ctccaggaag cgcgacaatg
     4741 gttttcaacc ccacacgcgc attgggagct cctgcaacga tttgtacagt cttatcactt
     4801 gcgactgcaa cttggcagat gttgaggtgg tcactatctg gatgggctac catctcaaca
     4861 atttcaccga caacaaactt aggttcctta tcattaacaa tttcttctgt aaaaccttcc
     4921 gcctgcaact cttggttcaa acgagcgact tgctcatctg tcaaaaagac ttgaccgcgc
     4981 tctgcagttt caaataaact tgaaacttcg aaaatattcc aagccactgt ttccccattt
     5041 tctttgagga aaacacgggc taccttgcct ttgcgttcca cattcagttt ggcatctccg
     5101 ctatttttca cgatgaccat aaggacatca ccgacatgtt ctttgttata cgtaaaaatc
     5161 attgtttcct ttttctccta tttcaatcct gctaaaaaat cgttaatttg ctccttactt
     5221 ttacggtcgc gattgacaaa acgaccgatt tccttgtcct tttctagaac aacaaggcta
     5281 ggaattccgt aaacatccca gagtttggcc aaatccatat actgatctcg gtccattcga
     5341 ataaaggtga actctggatt ggtctcttca atctctggta aggcaggata aatataacga
     5401 caatcgccac accagtctgc cacaaaaagg aagaccttct tgcccgcttt ttccactaaa
     5461 gatgctaatt cttctaaact tgctggctgt atcataagac ttcctcctca tagactaggt
     5521 cttcattttc atagacaaag gtataatgac ggccatcctc aaaaatgacg ccaccaacca
     5581 agctctccag actgctttcg taaacttgaa cataaagggt cgcaatttcc cccatgtcgg
     5641 aaaaatggtc tcgcacaatc tctgtcaact cttcctgagt cttcatgagc ttacggtcat
     5701 ctgcaacttt tttcgtagca agggcaaggc ttccgatacc tagcagagcc aagcctgcca
     5761 tccacatttt tttagctttc ataccattca ttttaacaca aaaaaggctt caggacaaat
     5821 gaggaagcag cagaaaagca agtaaaaagc ctcttccttt aaggaaaagg acttcttata
     5881 ctaagagcaa actagaaagc tagccgcagg ctgctcaaaa cacggttttg aggttgtaga
     5941 taaggcgacg ttgacgtggt ttgaagagat tttcaaagag tattattctt attgccaggc
     6001 acctaagttg ccaacgtagt aaccatcagg tgtgtaggta ttgcgaagca tcttacctga
     6061 tgaagccaga taatactact tgccattgtc tttgacccaa tcattcgcaa tcatggaacc
     6121 agaagaactt acataatacc attctccctt gtcataaacc caagtactga ctttcatggt
     6181 tcctgagcaa ttaaaggcaa aaaaactgtc caatatcatt cgttttttaa aagcattcga
     6241 cactacatca tctacgctgt agtacataga tacatcaaac gtaaaagcca aaatatcgaa
     6301 aaatatacaa cttttaaagg cagcatttca ttttttaaag tctaacaacc atagaactga
     6361 aatcagagca aggcatcaat ttcctactct atagtacgcc tattttgatg gttgaagtga
     6421 agatttttta tttaacgtag acaatttcca acttcccaaa agccacatct ccacggataa
     6481 tcaaggtttt gctggttggg gctacaggag cgtcagcctt agctgcacca aaggaggttt
     6541 ctactttcaa atctacacgc cagtgttgtg gaacatagac ggttgcattt ccgaaggcta
     6601 cttcaatatt taaagttgca aaatcaccta gcatctctgc attatcatag tagattttag
     6661 catccccaaa agcgacttcc acttggtcat ctacgagatc ttgatcttgc ttatagaagg
     6721 tcccgctacc aaaagcgact tccttatccg tgacgactgt ttttttccca ttgtaccacc
     6781 attttttttc attccagaac ttacttgaat gcgtcagata accaacacca agtaccacca
     6841 agatgctagc ccaaataaga gaatgattgg taactggtaa caagtcataa gcgtaatttg
     6901 caatgatgag cgccagtaaa cctgtaaaaa ctgccgaagt gagatgacgt ctaaggatgg
     6961 actcaatgga cttataagca aaaaaaacaa tacctagtaa aggccatatt ttaccatcca
     7021 aagaaggaat tccaaaattc ccttgcagca agatccaagc tgctaaaacc aataaaacaa
     7081 taccaaatgc tttctttttc atgtttttta cctcatgttt cttagatttt cttttaggag
     7141 ggattggtaa tgccgtgaga catgaacctc cttgtgcgtc tgataaaagg aaatggtgcc
     7201 cgttcctgaa aaggacttgt ccactgagta aatctgacgg atgtttgcga tcgttgactt
     7261 ggaaactcga ttaaaatagc gaggcaagat agactccaac tcatagagct tgaggcgaac
     7321 ctcataagct tccttctggt tatgagcgta gatcttgctc ccttctgttt caaagaagag
     7381 aatttctgac aggtctagat aatattcacc tgtccccttg taaaaagtca acctcggagc
     7441 ctttgactct tgcaagagcc gttgcaagtc tgcaatttca tctgtcaaag tgggggtctt
     7501 gatgacaatt tcagtctcct ctaaattgcc gtcaatctca attcgtaact tcatcacatc
     7561 tcctttctga ctctactata tcaaagctaa gttaggaata caagggcaaa aaagcaagtg
     7621 gtcaatttga agcagtaagt ggttgtagga ctagcttaac ttacatgtca gaataggaag
     7681 aaaaggaatc cccacaccaa accacaagca tagccaacca agacatcctt gggataatga
     7741 acacccccta aaaccctcac caaccccagc aaagctgaaa agatcaagca tactaagcca
     7801 ataggtagac tagcatgcag acaggccatg gagatgatag ttgccgaaaa gacatggcga
     7861 ctgggcatcg accgtcccga actatcctta tcaagaaggg gacagatatc ccaagtttca
     7921 taaggtctcg ggtgattgat tttcttacga aacagggaca aggtcacaaa acctgatgca
     7981 ggaataagca cataaacccc aacttgctga ccaagtccta gttgcaggta agtggtgact
     8041 agcaagatca gatagaccag gggcatggcg actgtcatca aacgattgaa actgcgtaat
     8101 aggaataaaa gaattggacg actagttagt ctggagctga tatttcgata ccattcttga
     8161 taatttttca tttatgcttc tcatactttt ctagaacttg ttgagatgtt ttccccttgg
     8221 caagccaatc cactagcttg tccaagcgac gtatttcctg catcagagga tcatcaattt
     8281 cctcaatacg gacaccgcaa atcttccctg taattgcaat tcgttcaggg acataatatg
     8341 gtgcttgtct aaaaaaatct ccgtaagtaa catccctatc cagacaagca agaacatcac
     8401 tcacttcata ccctgtcaac caactggtca cttggtgaac tgactctgcc tttccaccct
     8461 ttcgctcaac ttttgcaatc aaggctaagt agacagcagc aaatttcata ttgtataatt
     8521 tctgactcat acttctctct ttctatgtgt actagtataa ataagaaaaa gaaggccgtc
     8581 aagccttctt ttgatttatt cttctgcttc atcttctgta aattgactat tgtacaagtc
     8641 agcgtagaag ccaccttgcg ccatcagttc ctcatagttg ccttgctcga tgatatttcc
     8701 atctttcatg accaagatca agtctgcatt tcggatggtt gacaagcggt gggcaatgac
     8761 aaaggatgtg cgtcattcca tcaaacggtc catggctttt tggatcaatt cctctgtccg
     8821 tgtgtcaaca gaagaagtcg cctcatccaa aatcaaaagc ggtgcatcct taagaagggc
     8881 acgagcaata gtcaatagtt gtttttgtcc tacagacaag gtcacggtgt catccaagat
     8941 ggtatcatag ccatctggca aggtcataat aaagtggtga attcccacag ccttactagc
     9001 ttccatcatt cgttcatcac taatccctat ttgattatag atgagattgt ctcgaatagt
     9061 tccttcaaag agccaggtat cctgcaagac cattgaaaag gcatcatgca cttctgaacg
     9121 cgtcatagcc ttggtatcca caccatcaat gtgaatactt cccttatcaa tctcatagaa
     9181 tttcatcaaa agattgacaa tggttgtctt accagtccca gtcggcccaa caatggcaac
     9241 cttttgacca gcatgagctg tcgcagagaa gtcatagtct tgaacattga caccgtccac
     9301 cagaatttct cctgctgaca cgtcgtagaa acgtggaatc agattgacca gagttgattt
     9361 accagaacct gttgacccaa taaaggccac tgtttgacca gtttctgctt taaagctaac
     9421 atgttcaata actgcctccg aatttgccgc atagcggaag gtcacatcct taaactcgac
     9481 ctgacctttg aagttttcat cagtcagctg cacttgaaca gggttttgga tagaagaatg
     9541 caaatctaaa acttgattaa tccgcttagc agagaccata gttcggggaa gaacgatgaa
     9601 gagtgctccc atgagaagga agcccatgac aacctacatg gcataagaca tgaaaacaat
     9661 catgtcacta aagagaggca gacgcgctat cgaagcagcg tcgttaatca cataggcccc
     9721 aatccagtaa atcgctacac tcaaaccact tgaaatcccc atcatgatag gattcaaaat
     9781 agccataaga cggttgacaa acaaattcaa acgggtcaat tcatcattta ctgctgcaaa
     9841 tttttcattt tgataatcct ctgcattgta ggcacgaacg acacgaatac ctgttaaact
     9901 ctcacgagtg atactgttca gtttatctgt caacccctga atcaaggact gttttggaaa
     9961 ggctagcgtc atcaaaacgg tcgtcatcag gacgttgaca atcactacca caagtacggc
    10021 ccagagtcag tattctgaat gacctaaaat cttcccaata gcccagatag ccataattgg
    10081 accacgcgtt accacttgca agcccatagt aatcaacatt tgaacttgag taatgtcatt
    10141 ggtagtacgc gtcaagaggc taggaattga aaatttctta atctctgtct gcgagtaatc
    10201 caaaactcgg ttaaaaatat cacttctcag cctactagta taagaagccg ccactcggga
    10261 tgcaaaaaat ccaactgcaa ctacggacaa gaaggcaaga aagagcattc ccatcatcat
    10321 gcttgccggc tgccacaact catctaaatt agtttcttga ctacctagca aatccgtaat
    10381 tttcgagata taggtcggca cttccaactc tagatagacc gaaaagcaag taaagaaaat
    10441 ggctagtaaa atcatccccc attcttttct actaattctt ttggttaatt tctttattct
    10501 ctcctcctat tcccttgata ttttgcctgt agttgaccga gaactttctc aaaaatcagt
    10561 aattcatctt catcaatgtc ttccatcaac tgcttgtcta tgcgttcaaa aaaagcctta
    10621 acctgttgca tctgagaacg tgctttgtcc gtcagacgaa caaacttagc ccgcttatca
    10681 acaggactcg cctccaattc caccaaacca ttttgcacta tacgcttaac cagattacta
    10741 gcaacagact tggtaatatt gagttcctgc tcgatatctt taatcaagac caagtcttgg
    10801 tttttctagc gattatccaa aaaacgcaca acctgacctt gcggcccacc cataaattca
    10861 atgccgcaac gtttggcttc cttttgcacc atcaggtgaa tttgatgacc aaaacgctta
    10921 aagactaaca tcggtttatc cataatctcc cccttctaaa taaaaatagt tctctggaga
    10981 ataattaaat ttctatgaga actattttct tgattaaaaa aatcccaagt gattttctca
    11041 cttaggatca tgttctatag gttaaattaa aacccatcta cgttcgtata aatcttttgg
    11101 acgtcttcgt cgtcttcaag aacgctgtaa agtttttcaa aggtttcaag gtcttcgcct
    11161 gacaattcca cttctgactg aggaatcatt tccaattcag tcacttggaa ttcttcaata
    11221 ccagactcac ggagagcaac gatagccttg tgaaggtcag ttggcgctgt gtaaactgtg
    11281 attgtacctt cttgtgcttc tacgtcatcc acatccacat ccgcttcgag caattgctca
    11341 aagactgcgt ctgcatcttc acctccaaat acaataacac ctttgttgtc aaagaggtaa
    11401 gaaacagaac ctgaagcgcc catgtttccg ccgtttttac caaaggctgc acggacattg
    11461 gctgctgtac ggttgacgtt agaagtcaaa gtatccacaa ttagcataga gccatttggc
    11521 ccaaaacctt cgtaacgtcc ttctgtaaag gtttcgtctg tgtttccttt ggctttatca
    11581 atcgctttat cgataatgtg ttttggcact tgggcttgtt tagcacggtc gataacgaat
    11641 ttcaaagctg agtttgattc tggatctgga tcaccttttt tagctgctac atagatttct
    11701 acaccaaatt ttgcatatac cttagagtta gctccatctt tagccgtttt cttggctacg
    11761 atattggccc atttacgtcc cattaggaat ctcctttttt cacattttaa tctttattat
    11821 tataacacaa gtttttttga ttttcactag aggaaatgga ttttattagc aaatcaagct
    11881 aggatagcac tttacctgct aagatggtct tacctttcta tctttatcaa cagacactca
    11941 tccacattca aaaaacaaac tagaccatta tctgcaaata gaaagtttca gccaagtttg
    12001 acaaagtcag ctcaaattac tgtttgaagt ttgtagatat aagcgacaaa aacaatcata
    12061 ctgcaccttt tgttgacagt ctactccaga catatcatag ttcaagtaaa tactttgaaa
    12121 ttcaacagtt cttataggcg ctattgtatt ctaagaaatc aatagaagag tttctaagca
    12181 aacctctaat actcaataaa aatcaaagag caaaccagaa agctagccgc aggttgctca
    12241 aaacactgtt ttgaggttgc ggatggggct gacatggttt gaagagattt tcgaagagta
    12301 taatttacgt gttcccaaga tggagaagtt agactagtac actggcactt ctaaaccatt
    12361 gctagcaatt gatttgttca tatttaattt cattttttcc ataaatgggt attagatata
    12421 aacagcaaaa tatttccgat acgtgtcgtt cttgaatttc caatcatcta aaacaagtaa
    12481 aggataatca atcccctgta tatcaaggaa ttggctaccc tttttacttt tttacacatt
    12541 ctgtttgata gattcatttt aacatcacga gcatactcca atggaaatcg ctaggcaaga
    12601 gataaacttt cagatatccg cagagagatc atcgcctctt tttgtcgcaa gcattctcct
    12661 ctcctagtca ttttctacct tatcttctac ctgaggatag agagttgttc cccaaataga
    12721 aatcgtccgc ttacgcacta gtggcaaatc ggttttttca taaaccgtac gccaccattc
    12781 ccaggcaagc ccggtacact ctctaatttt gacagagaga ttacgaacat tcccttttaa
    12841 aggaatacta gtggtaaagt gagccgttaa atcctgccca tttctgtccc aagccttagg
    12901 agtcaagact tccttacctt gatgatcata ggataattca ttccaagtaa tataatattg
    12961 ggcaacatag gcaccactat gatccagcag taaatctccg tttctgtaag ctgtaacctt
    13021 agtctcaaca tagtctgtac tattttgaaa ggtcgcaact acattgtcac gtaaaaaaga
    13081 agttgtatag gaaatcggca agcctggatg atctgctgta aagcgactgc cttcttgaat
    13141 caagtcctct accatatcca ccttgcctgt tacaactcgg gcacccgaac ttgggtcgcc
    13201 ccctaaaata accgccttca cttctgtatt gtccaaaatc tgtttccact ctgtctgagg
    13261 agctaccttg actcctttta tcaaagcttc aaaagcagcc tctacttcat cactcttact
    13321 cgtggtttcc aacttgagat agacttggcg cccataagca acactcgaaa tatagaccaa
    13381 aggacgctct gcagaaattc ctctctgttt taaatcctct accgttacag tatcttgaaa
    13441 cacatctcct ggatttttaa cagcatctac gctgactgta taataaatct gcttaaaatt
    13501 aacaatctga atctgctttt cgcccgaatg gacagagtta aaatcaatat caagagaatt
    13561 ccctgtcttt tcaaagtcag aaccaaactt gaccttgagt tgttccatgc tgtgagccgt
    13621 gattttttca tactgcattc tagctgggac attattgacc tgaccataat cttgatgcca
    13681 cttagccaac aaatcgttta ccgctccgcg aacacttgaa ttgctggggt cttccacttg
    13741 gagaaagcta tcgctacttg ccaaaccagg caaatcaata ctataagtca tcggagcacg
    13801 atcgaccgca agaagagtgg gattattctc taacaaggtc tcatccacta cgagaagtgc
    13861 tccaggatag aggcgactgt cgttggtagc tgttacagaa atatcacttg tatttgtcga
    13921 caagctccgc ttctttcttt cgataacaac aaactcatcg ggtagctgat taccctcttt
    13981 gatgaaacga ttttcaatac tttctccctg atgggtcaag agtttctttt tatcgtaatt
    14041 catagctagt ataaagtcat ttactgcttt atttgccatc ttctacctcc taataagttc
    14101 ctggattgag ttgcataaac tcagacttgt tcagcgaaat cagccgtggt tggactaagt
    14161 aatccaaaat ttcctcgtac aattcttctg agacattgcg tcgccgtctg gctagataag
    14221 aagtcggaat gcccgtatta tccaacataa ataccttatc taagtcaatc aaggttggtc
    14281 ttgtaaaagg attacgagct agatccggct cttctatcat aaagttcttg accaaacgtc
    14341 tggtcaagag agctggtttg aaggtctgat ttttaaccaa ctctttgttt ttagtcatgc
    14401 tgttgtcaat acagatatac atatgattct tcacagccaa atcgctacta atagtcggaa
    14461 aaggcaaata aagagctaca acatctcctc tcttaatcaa gcaagagcac ccccttttct
    14521 cctaatgtaa catagacagg attgaccaag tcttctgatt gactcagaat ttccaaagtt
    14581 tgagtttggc gcgctgtcaa tttagtagca tcttgtctct tcaatacaaa atgcttgtcg
    14641 ccaataacct tgacaatata atccttctcc aaagctgact ggtaaatcca catcagatgt
    14701 tgtctgtcct gagaactcaa gagagaagga ttttcaagcc tcccgatagt ctgataaaaa
    14761 tcaaaaacag gagctaactc ctgccaatct gattggctag ttgtcaaggc tagaaaaagg
    14821 gctttgcgag ctgatacttc ttggttagcc ttgagagtta ctttcccctc caagtttttt
    14881 agaaatcggg aaactccaga aagcaaattt ttctctaact gcgagaaata aaaacctttc
    14941 gttcccagac ataagtcttt catgtcgctt tctctagcaa ataagagctc aaacatttga
    15001 tagtaaaaga aaaatatctg gcactgggtc gcgctcatct tttccttatc ggcttctttt
    15061 tttaaccaga gcaagggcga caggtaactg gattgagaca tttcctctac ctcctactct
    15121 tttttaactg gagcatctgc actagctgcc acttcttttg actggatact ttcccactgg
    15181 ttaatctcct ctgagataag accttcgcat gtcttgacaa atagggcaaa agccttggtc
    15241 tttcctgcat atttctccgt ttggcattga tagaggaatt tttctttctc caggagttgc
    15301 gcagtttttt ggtaagaaat ccaattttcc tttgcattat acaaattgat aatcccctca
    15361 cacagcaagc cgagactgga taaggcaacc gaaatcaaac ggtagcgatc acctggcata
    15421 ggaatagcac aaaagacagc tatgaggaaa cctgccacga tttctgttat ttttaatacc
    15481 ttatagcgcc tacgatgttg aacgcttttc tttaaaaaat gagctatctg tacgtctaat
    15541 cgctctgtca ggtacatttc ttctggcgtc atattcgtaa ctcctttcat ttactttgat
    15601 aaccatagta taacatattt gaatatcaaa gtatagtaaa aactggacta taacttatta
    15661 gacttcctgc gaaacaaaat atggtatagt agttctatga attatgaagc aagtaaacaa
    15721 ctaactgatg cacgatttaa gcgtcttgtt agtgttcagc gtaccacttt taaagagatg
    15781 ttagctgtat taaaaacagc ttatcaaaaa agtcggacaa gctggtaaaa tcttggctga
    15841 cagtggttat caagggctca tgaagatata tcctcaagca caaattccac gtaaatccag
    15901 caaactcaag ccgctaacag ttgaagataa agcctgtaac catgcgctat ctaaggagag
    15961 aagcaaggtt gagaacatct ttgtcaaagt aaaaacgttt aaaatgtttt caacaaccta
    16021 tcgaaatcat cgtaaacgct tcggattacg aatgaatttg attgctggta ttatcaatca
    16081 taaactagga ttctagtttt gcaggaagtc tattatatat atgcacagac atatgaaatc
    16141 tttttgatac agccttcttt ttacatagag agacagcagt tatgatatat ttatccattc
    16201 acaaaatctt ctgtaccaat actacaataa ttggtacatt ttgattacac aatcgtttca
    16261 aaaaatatga tggaagactg agtattttat agtaattccc ttcctatcaa ctagatcaac
    16321 cagttgagtg ttgaatgttg aaacacccta gcctcagcaa aggactatga tcagcatttc
    16381 ctagccaata ggacgctcca aatcacctat tctaagtcca tcactagctt ggtcagtaag
    16441 atacagactg caaggcaaga ctgacgtggt tgatgagatt ttggaagagt attacaatca
    16501 tagattcacc agtagacttg accgcagatt taactatagt agattgaaac tagaatagta
    16561 cacttctgct tctaaaacat tgttagaaat cgatttgact gtcctgatca atttgtcctg
    16621 ttctcgtttc attttactat actaaaaaac cacctgaaac aaatcaagtg actagaggat
    16681 aaaaacaaag actgggcaaa aagttttgaa aagcaaaaaa cgcataatat caggtcttga
    16741 aaaaccttgc tactatacgt ttgattgtgg gaagattgac ttcattttct cctgaaattg
    16801 aagttttttc tagtttccag gtatagacga attcaatata gccatctcgc tacaattatt
    16861 ttaaccatcg aaaaaatttt tcagaatttc aaaaatttca ttttgatcca atgaaatgtc
    16921 attattttga agaatagttg acttcacagg ctctaattct atcacaaagc ggtgcatgtc
    16981 tgcttttacc gcactacttg atgttatagc cgataacttt tcatctccta gcaaggaagc
    17041 tgtcaaacgg gtaaggtcat tcaaatgctt tttaatggac ttacttaaac cttgagcacc
    17101 tgtggcagag cgctccctca tatccagcca agctttagcc ttaaaaataa ttaatactca
    17161 atgaaaatca aagagcaaac taggaagcta gttgcaggct gctcaaaaca ctgttttgag
    17221 gttgtagatg gaagctgacg tggtttgaag agattttcga agagtataga ccacaattac
    17281 tcaataccga atacccctga atggtttctt ttaaatgcac caatatatta taataatctt
    17341 catccaataa taaggctgat aaactagcat cttggtcaaa atataaggga atttctcgac
    17401 cgtccttctt taatggatat tctggtaaga tactaaatag ttcaatcata gaaggaaact
    17461 cagaattagt tgttgtaaat cgaaaaagct gcgctttctc atctttctga cttccttgat
    17521 actctcccaa ttctaaaaaa tgattcaagg tagtataaaa ttccttattt tttacttcat
    17581 caatgatgac catatcataa tcttttgttg tgcgactttt aaatccttgc gaatccaata
    17641 cgatagaggt agcagttccc ccaatcagaa cataatagtt ctgaaaatcc gcaaacattt
    17701 cttgaaaaat aactttcgta ttagctggca tcatcttctc ccagatactg taatatcata
    17761 ttttctagtg cttcactctc ttcctctata cgtgggtcat catcatcttt taaggtcaaa
    17821 taaagagaaa tcggatctac aaattgttta tcatgattat ttttaaaatc attccaaaac
    17881 tcagatacaa aaggacgata tttccatatc tctagcatct ttccttttaa aatatgctga
    17941 gaaagtggca aggataactg attgaatttt ctctgccata tgacatagct agtattttca
    18001 tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag caccaccata tagaaggtta
    18061 gaaacagatt ttatctgctt tatatcgcca tctggcaata aaatccgttt tttgatggga
    18121 ttaaataaac atgacacgga ttttaagaat aattctttct ttgacaccgt atatgtgtaa
    18181 agcttatttt gcttgtttaa ccaatataaa gctttaaaag tcctcaaaca cctataaatt
    18241 gttgagtttg gaagtccagt gacttgtgaa agcaaatcaa catctactac tttttgacct
    18301 tttgtcaata aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggact
    18361 tcagtatcat tcgcattgag tactagtccc aatggaggga agaagaggtt tcccttaaag
    18421 tctacaaacg gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa
    18481 aacaccaaaa caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat
    18541 gaactcaaac tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc
    18601 atttgagtat actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca
    18661 atactgacat caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt
    18721 tttccttatt ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt
    18781 ttgataaaat aaccaaaaac tactccctat cccctctcac attaccctac atatcgtttg
    18841 acatgcgact gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag
    18901 gacttactat ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg
    18961 ctcatatgcc tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat
    19021 gaatacttcc acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa
    19081 gcatattatt ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt
    19141 caatcagtat atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatgatttaa
    19201 catctcttag gttattactt cctaatatcc cattatgctt tttcgctaag gaaatagaag
    19261 ccgcttctcc cttaccaata atcttgttac tatcatgatt tcttgttaaa tctctatata
    19321 atgcgtattc ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagctacca
    19381 actgatctat cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct
    19441 cttgtggaat aacaatttta cccgaataga gcttttctaa aagatgttca gtaccaaccc
    19501 ataaaaatac tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc
    19561 cccctcttca tctagcccat atacaatatc atatctgaaa gcatctagta acagttcctc
    19621 atacttccct tgcgaaattc tgttattttc taaaagttgt tcagttgaat taacatatta
    19681 tcctaatatc atttactttt tactttctga caaaggacga tataaacttg tatcatagcc
    19741 taatcttgaa gctgtctcta taacactaat atccatattt ttaatttctt ctgcatcaag
    19801 gtatccatca ttcctcaatc tatataacat aactttatga ctgataccat aaaactgacc
    19861 caattttata atatcttcta cttcaagatg agttctattg gcattttctc taatttcctc
    19921 aaccatccta tacagtgaag atgggaaaat taaaaaataa gaagcaaact gatccgcttt
    19981 tctttcagtt tcatctcctt caccaatcaa gataagactg actgaactct tcttcacctc
    20041 atcataataa agatgataca gttcatgtgc taaagaaaat ctttaccttc ctaatgacat
    20101 gtctgaattg actgcaatga gactgaactg agttccttta taacagactc cgctaatatt
    20161 ctttccgagt ccataaaata ccagcgtcaa attttctatc ttttgtacca atttaaaaat
    20221 atctatcggc gattcaccat cagctcccaa cttttttcta agatttgaag ctttattact
    20281 taaatccatt cgtgatattc ctttcacctt tttccatttt ggcaatcatg ctttgattca
    20341 aagacaaata ctcagcaatc ttatcttgag taagatttga agatattcta agttctttta
    20401 ttctttttcc aacatcacat acatttatca catacattta tcatattcat acacctctca
    20461 taaaaagaat agcacacttt tgtcataatt ttttaaataa aaaaattatg acaaaacaag
    20521 gaagcaattt attgatgctg cttaaaaatc taaaattgat gagttcctat caaaaatcgt
    20581 atcttcaacc tcaaaacagt acttaaagct atccgactcg gtttacattg tcaaatttag
    20641 attttatttg agcataactt tctagtttgc tttctgattt ttgtttaata tagtagcaaa
    20701 cattgaagga aatatctcca caaaaaaacg catactatta agctttttca agacctaata
    20761 atatgcgctg ttctgatttg aaagacattc cattattatt ttactgtaat caagccatct
    20821 ggctctactg tgaattctgg cttgtctgcc agtgttccgt ctggtttgag gtagtaccag
    20881 cctgttccgt ccgctgactg gataaaggca tttgatacca tggcgccttc tttagcgtct
    20941 aagtagtacc aagtgtcctt gtacttgacc cagcctgtct tcatggcacc ttcttcgttg
    21001 aaatagtacc acttatcagc gattttcttc cagcctgtag ccatttcgcc tgagttgtcg
    21061 aaccagtacc agttgccgtc tgtgtgcttc ctccagcggt ctgcaagcat atagcctgaa
    21121 ctgtcaaagt agtaccaagt gccattgatt ttctcaaact tgtcttttgg ataagagccg
    21181 tctgaatgta cgtaccagta gccagtgtca ttcttctgcc agcctgtttc aatcgtcaag
    21241 ccgttctcaa tatcatactt aaactgctca cggctaatgc cccattttgc caagtaaggg
    21301 tatggatcca catggtctga gtggttgttt ggttggttat tcgtgcaata ctcgtgcgtt
    21361 ttaattccag ctaaactccc tgtatcaagc gttttcggca aacctgcttc atctgctaga
    21421 ttgcgtaaga gttcgatata aaggcggtag tccgtcatga actcttcttt agttgaatgg
    21481 ctttcaatca gttcaaccgc tgcataggtt tcagcattcc aaccgccccc aacgtcccag
    21541 gcaccattat caacaggtcc tacctgcatg atgcaaccgt tcccaacaat gtgcgagaaa
    21601 aaacctaatt ctgggtcttt ccgccagtga taatccgctt cattctgtac ggttgaatgc
    21661 ggattcccag ttgagtgtgc gtgtacttgc ctatatggtt gcacgccgac ttgaggcaaa
    21721 tctgttctta atttactcac attaatttcc atattctact ccttatcaat taaaacaact
    21781 cattttttac aatccagaac cagaaactcc tttatttcta ccttacaaag aagacaatct
    21841 tagtcacgat taggcttgta gatagaacct caaaacgcac tattttgaca ctgtaaatag
    21901 gactgacaag gtctgcattc tatctacaat aacaccccag actaaaaagc ttttcaaaag
    21961 tatattttta cagtctctat atgtcctttt cataaatact atactttatt atatcatata
    22021 aaagaagtca aaagtctgtt aaactatttt caacaccaaa ctaaagaaga gaacacaagt
    22081 tttttcgatg ttcactagag gaaatggatt ttattcagta aatccaacta ggattgcact
    22141 ttggttgcca aaattgcctt tccttctttt atcaagggat gacggaatag tgagaagtac
    22201 agttgaactg tcatggcaac ccagataaga ggttcacaaa ggataacacc cttatatcct
    22261 gcccaaggaa taatcaaaac cacaaaaacg atttttccga ttagttcaat aaagctagaa
    22321 actagaggaa ggatcttctg ccccaagccc tgcaagcaat tgcgataaat caacaagagg
    22381 ctcaaaatgg gataaaaggc tgaactgatt tgcagataga gacttccatt ttctatcaag
    22441 taaccatctg tcgaactagc caagaaggaa accaaggctg gactggcaaa aaagaggaaa
    22501 atacaaacaa aaactgccca ggatatactt aaacgactgc cgattcgaag accttgaaca
    22561 atgcggtcag gtcgcttagc tcctagattc tgagaagcaa aggtcgtcat tgatgcagaa
    22621 atagcggtca taggaagaag ggcaaaggtc ataatgcgtc gagctgccgt ctgggcacta
    22681 ataatcactg caccaaatgt attaacagaa aactgtaaaa tcacactgcc gatagataca
    22741 attgaactca tcaagcccat agccaaacct tgctccaaga gatccgcgta caagcttttg
    22801 tcccatttga aatgtttaaa ctgtggcaag agttctggca cacttttacg aatataataa
    22861 aagcagagaa ccgctgataa accttgcgaa ataatggtag caagtcctgc ggattgaact
    22921 cccagatgca attgcgtaat aaaatagaga tccagaacca cattaaccaa ggcagagaaa
    22981 atcagaaatc ccagggctgc tagactgtca ccaatagacc gcaacaagcc tgcaaaaaga
    23041 ttataagcaa agctgacacc tacacaggtc acaatcatag aaatatattg ataagattga
    23101 ggaagaattt ctgcaggagt atctaagtat tgcaagagag gatacaagcc aagaaagccc
    23161 agcaacataa ctagaatgct caaaagagca cctaaaatcc aggtggctgc tactgcttcc
    23221 ttgattttag tgaaattccg agccccataa taacgagcaa tgacaatccc catgccattg
    23281 ccaacaccaa gtgtaaagcc tacaatcagg tcaaaaatcg ctgtcgtcgc tcctactgca
    23341 gccaaggatt cttgaccaag aaatcgtcca acaatcaaga catcagcagt gttatagagc
    23401 tgttgaaaaa tatttgatag caagattgga aaggtgaagc ttaagagcga gggaagaatc
    23461 ggaccatgta tcaggtccac tgttcgtttt ttattcataa agctattata tcagctttcc
    23521 agaggagcga caagaaacag caaacttata tatagcggaa tgaaataagg acagtcaaat
    23581 cgatttttaa caatgtttta gaagtagatg tgtactattc tagtttcgat ctattatagt
    23641 tggttgagtt tggaa
//
LOCUS       DAVLFB010000025        22893 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLFB010000025 DAVLFB010000000
VERSION     DAVLFB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.025"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            5..190
                     /locus_tag="VXD58_000861"
     CDS             5..190
                     /locus_tag="VXD58_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV0675592.1"
                     /translation="MIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYGKGSDYV
                     KEKVVDILGIENPWENS"
     gene            207..404
                     /locus_tag="VXD58_000862"
     CDS             207..404
                     /locus_tag="VXD58_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675593.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            complement(520..1179)
                     /locus_tag="VXD58_000863"
     CDS             complement(520..1179)
                     /locus_tag="VXD58_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675594.1"
                     /translation="MTERICFIVTAIGESGTETRDRADEVFSYLIAPVCEELGYKPVR
                     VDQVDAVDNINETIINYLKTAPMVVADMTGHNPNAFYELGFRQAKELPLVPIIQAGNG
                     LPFDVISQRTVFYNLSVGKIEQSKRELKAKMKSFENFEMPESRIDKSVTLEELDDKLT
                     KKLNKILNLLESQQSYSSRIHTYDFNAKPTIGDHQSITQRVQEIINQTHNRPLFPEDK
                     K"
     gene            1233..1946
                     /locus_tag="VXD58_000864"
     CDS             1233..1946
                     /locus_tag="VXD58_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV0675595.1"
                     /translation="MNEIFNFHGQEVRTLIIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQASMIEDIDYLKNEQPIHPSYAQSLLKKRKA
                     RVVACLGGIDSPAYADKIFAQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMTW
                     EPSTNTKMKIMKLNSFDEG"
     gene            1959..2216
                     /locus_tag="VXD58_000865"
     CDS             1959..2216
                     /locus_tag="VXD58_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0675596.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            2302..2622
                     /locus_tag="VXD58_000866"
     CDS             2302..2622
                     /locus_tag="VXD58_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675597.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGAWRHIKLQEQRKEDERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPQGLRPEPLALPEPKQSA
                     IRFL"
     gene            2638..2931
                     /locus_tag="VXD58_000867"
     CDS             2638..2931
                     /locus_tag="VXD58_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675598.1"
                     /translation="MEELIEWLETKEKLNREMISDEETSDFNLHLENDNDTMFMIKEY
                     LNDYNKLAKDYRILSSENRLLKLEKMELEGRHIYEDMRMKYRANRRKWGARYV"
     gene            2924..3757
                     /locus_tag="VXD58_000868"
     CDS             2924..3757
                     /locus_tag="VXD58_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV0675599.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALDKELDKDKELDKD
                     IEINNNKVMISSSLSENLKNSGIHLTNKSHQQLLEYVGLDGMSFDMLNRAVEKTSGSH
                     KPSFNYLIAILESWKKKGFTSIEQVDEDDRKYKEGKNYNHSRQQNNEISEQEVKDEWG
                     F"
     gene            3745..3903
                     /locus_tag="VXD58_000869"
     CDS             3745..3903
                     /locus_tag="VXD58_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675600.1"
                     /translation="MGILELIEQFEDEFYPISDEKKSLLIKQPLSTVTACLSEMASWR
                     ECGGSLSW"
     gene            3897..4667
                     /locus_tag="VXD58_000870"
     CDS             3897..4667
                     /locus_tag="VXD58_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0675601.1"
                     /translation="MVDNVFEEIALSYHRSTEQQDEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQVRVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKKICREWSEGVRNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            4682..4876
                     /locus_tag="VXD58_000871"
     CDS             4682..4876
                     /locus_tag="VXD58_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675602.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     LQVSKALRGSFGNFYPVEVE"
     gene            4876..5103
                     /locus_tag="VXD58_000872"
     CDS             4876..5103
                     /locus_tag="VXD58_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675603.1"
                     /translation="MLELYFVYNGHCKFYLGTFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            5230..5397
                     /locus_tag="VXD58_000873"
     CDS             5230..5397
                     /locus_tag="VXD58_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675604.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            5384..5593
                     /locus_tag="VXD58_000874"
     CDS             5384..5593
                     /locus_tag="VXD58_000874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675605.1"
                     /translation="MKYDKQAEIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LRKKNKELRRKLEELEDDGRVKAKS"
     gene            5565..5894
                     /locus_tag="VXD58_000875"
     CDS             5565..5894
                     /locus_tag="VXD58_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675606.1"
                     /translation="MMEELKQKVNAVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGSEDET"
     gene            5884..6387
                     /locus_tag="VXD58_000876"
     CDS             5884..6387
                     /locus_tag="VXD58_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV0675607.1"
                     /translation="MKLNELIKKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYEVEKE
                     KRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCPG
                     IEIEEVE"
     gene            6387..6902
                     /locus_tag="VXD58_000877"
     CDS             6387..6902
                     /locus_tag="VXD58_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0675608.1"
                     /translation="MGDIRILDACCGSRMFWFDKNESHTTFMDIRQEKFEIYGKKVNV
                     NPDVVGDFRDMPFDGETFNLVVFDPPHLKWAGQNSIMRSQYGQLDKVTWSEDLAKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLKSVPFKPLFGQQRGTTHWMTFMKFEKVTERK
                     DGTFVKEEDLA"
     gene            6899..7288
                     /locus_tag="VXD58_000878"
     CDS             6899..7288
                     /locus_tag="VXD58_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675609.1"
                     /translation="MIPKFRVWHHELGRLMSVKCMFFQDSEIEEFELNDALMNDYITA
                     YPDEIELMQSTGLKDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMPAVYLKG
                     LGDGYAWTEYEEIIGNIYENPELLEDK"
     gene            7288..7524
                     /locus_tag="VXD58_000879"
     CDS             7288..7524
                     /locus_tag="VXD58_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEV0675610.1"
                     /translation="MNPEIIDNINKPSHYQGANGLEAIDVVHNFVGSLSGASAFFWGN
                     AIKYMLRFQKKNGLEDLKKARKNLDWLIEEMEHE"
     gene            7521..7679
                     /locus_tag="VXD58_000880"
     CDS             7521..7679
                     /locus_tag="VXD58_000880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675611.1"
                     /translation="MNTHCMKATPLLPWGRLRKSAKKQELLNGASISEFAIEDICTFF
                     GIEIQERK"
     gene            7891..8292
                     /locus_tag="VXD58_000881"
     CDS             7891..8292
                     /locus_tag="VXD58_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV0675612.1"
                     /translation="MKKLSDEELKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     TVGISKPTETIAIRIADDPTLKFLEGFKGIINKLLSNLVEEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAIMKGEL"
     gene            8481..9023
                     /locus_tag="VXD58_000882"
     CDS             8481..9023
                     /locus_tag="VXD58_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0675613.1"
                     /translation="MEFVSPIKDNDNIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            9187..9378
                     /locus_tag="VXD58_000883"
     CDS             9187..9378
                     /locus_tag="VXD58_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675614.1"
                     /translation="MVKEHRDDFLGEKAFEKLNKDIDANPEVGFEIVGYTQTAFVNGM
                     HIPLTAILVKWNNFFKESE"
     gene            9498..9818
                     /locus_tag="VXD58_000884"
     CDS             9498..9818
                     /locus_tag="VXD58_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV0675615.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            9957..10349
                     /locus_tag="VXD58_000885"
     CDS             9957..10349
                     /locus_tag="VXD58_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEV0675616.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            10342..12072
                     /locus_tag="VXD58_000886"
     CDS             10342..12072
                     /locus_tag="VXD58_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV0675617.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFSCIQALANLG
                     KGGDVMRFFD"
     gene            12080..12298
                     /locus_tag="VXD58_000887"
     CDS             12080..12298
                     /locus_tag="VXD58_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675618.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            12316..13518
                     /locus_tag="VXD58_000888"
     CDS             12316..13518
                     /locus_tag="VXD58_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV0675619.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            13502..14077
                     /locus_tag="VXD58_000889"
     CDS             13502..14077
                     /locus_tag="VXD58_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV0675620.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            14074..15246
                     /locus_tag="VXD58_000890"
     CDS             14074..15246
                     /locus_tag="VXD58_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV0675621.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVSAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTYPSQEKRGGQ
                     KLEQRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSTDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGENTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            15258..15527
                     /locus_tag="VXD58_000891"
     CDS             15258..15527
                     /locus_tag="VXD58_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675622.1"
                     /translation="MSYKVIRPFKDLRDPKKHCYKVGDNYPRVKHKLDKEFAETLLNG
                     ANSAGSIFLMNVEDKDMSKDDTLTTEVEKEETKVVDGKPEEGDEE"
     gene            15530..15811
                     /locus_tag="VXD58_000892"
     CDS             15530..15811
                     /locus_tag="VXD58_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675623.1"
                     /translation="MDNVQLLELLKLKLGIATKLRDKSLEKIIEAVKTELEDNLGVLL
                     DLDSSEDQMFVVDFAAFRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            15798..16097
                     /locus_tag="VXD58_000893"
     CDS             15798..16097
                     /locus_tag="VXD58_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEV0675624.1"
                     /translation="MWNHEITLISKEVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            16094..16453
                     /locus_tag="VXD58_000894"
     CDS             16094..16453
                     /locus_tag="VXD58_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV0675625.1"
                     /translation="MSLTSDLANEIAKAMAEYSAEVEDKIDLIAEDVVNEAVTELKAT
                     SPKRHGKYARNWRFKKNAKGSYVIYNAAPTYRLTHLLENGHVLRNGGRSRAFPHIKPV
                     EEKVKENFEKRIKEIGK"
     gene            16450..16788
                     /locus_tag="VXD58_000895"
     CDS             16450..16788
                     /locus_tag="VXD58_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675626.1"
                     /translation="MKLSDFAAILEQVNLPVTYRAFKTGNAPDLPYLVYYESIPAINA
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWTTHELFFDVQEETFIETERLYVKSY
                     TVYLYQGGMT"
     gene            16785..17363
                     /locus_tag="VXD58_000896"
     CDS             16785..17363
                     /locus_tag="VXD58_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0675627.1"
                     /translation="MTQENKVTFGLKNVHVAPIKSIGADGVIAYDEIFRFPGAMELTL
                     DPRGESTPIKADDIDYHFMNSNEGYEGKFKISHIIEMFATKILGEIKDAQTGVLTEKA
                     DAESTSFALMFEFSGDKNKTRHVLYYCSASRPGNGSKTKNGTNVNERELGFKASPRPL
                     DSVIKRSITSADNKEIYDNWFKKVYEPTAVAA"
     gene            17375..17794
                     /locus_tag="VXD58_000897"
     CDS             17375..17794
                     /locus_tag="VXD58_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675628.1"
                     /translation="MRKIVWVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETHKELTATDIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            18072..20831
                     /locus_tag="VXD58_000898"
     CDS             18072..20831
                     /locus_tag="VXD58_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV0675629.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMVKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFSSLPAPVKDFVVILGTV
                     VTAVGVIAPIFLSLQALAEFLKISIGEMIIAALPIIGTAIAIAAAVAAIIVIVKYLWE
                     TNEGFRDAVTTVWNAILEVINAVVSEISNFIMSIFGTVVAWWTENQELIRASAETVWN
                     AIYTVISTILEILGPLIQAGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIIT
                     GDWSGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNVWNGIKDTV
                     SNVLNAISSTVSSVWEGIKSTISGAINGAKDAVSSAIEAIKGLFNFSISWPHIPLPHF
                     YVSGSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNSLMVGGEAGNEAVLPLN
                     DKTLGAIGRGIAQTMGGTSPTINITITGNTVREEADIIRIADEVAQRIADELQRKTQL
                     RGGFT"
     gene            20828..21550
                     /locus_tag="VXD58_000899"
     CDS             20828..21550
                     /locus_tag="VXD58_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0675630.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSIALGESKTALLEHGGI
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKHFKVIDTQRLTRSGTLNVQGSALAFPKITIVG
                     QSASETSFTIAGQVIRLERLTESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSLEIETVWGWA"
     gene            21551..>22893
                     /locus_tag="VXD58_000900"
     CDS             21551..>22893
                     /locus_tag="VXD58_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV0675631.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMTLSRMIQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKTVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAR
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLDSV"
BASE COUNT         7502 a         3820 c         5319 g         6252 t
ORIGIN      
        1 aaagataatc aaggctaaaa tcaaaactcg tttgattgag ctggatatga ctcaagccga
       61 attggcaaaa caagtatctg tagcatcatc agttatttca gaactgctga agtatggaaa
      121 aggaagtgat tatgtgaaag aaaaagtcgt agatattttg ggtattgaaa acccttggga
      181 aaatagttag agcatgagga aatagtatga acaacgcagc gcaaaaagta acacggattg
      241 acaaagatgc ctgggagatt gctacggagc tggcgaacga gtacggcgta tctatttgtc
      301 acatcatcag cgagagcgtc cgctactgtg cagagaatgc cgaatttaag gagatggacg
      361 ttgtcgttaa acgattggta gtcggcagta aggtgctgga gtaggagggg aagatgaatg
      421 aactagaaag aacagccctc aatgaagtat tgaggaccgt tagacttata aatgaaaaag
      481 ttgctgaggt tgttgaactt caaagtcagc aagagctagc tatttcttat cttcggggaa
      541 taatggacgg ttgtgagtct gattgattat ctcttgaact cgttgcgtaa ttgactgatg
      601 gtcacctatt gttggcttcg cattaaaatc atacgtgtgt atgcgagaag aataagactg
      661 ttggctttct aacagattta gtatcttgtt tagtttttta gttagcttgt catcaagctc
      721 ttctaatgta acgcttttat caatgcgact ttcaggcatt tcaaagtttt caaagctttt
      781 cattttagct ttcaattctc ttttggattg ctcgattttt ccaacagaaa ggttatagaa
      841 aacagttctt tgggatataa catcaaaagg aagtccatta cctgcttgta tgataggaac
      901 taaggggagt tcttttgctt gtcgaaatcc taattcataa aatgcattcg gattgtgtcc
      961 tgtcatatct gctacaacca taggggcagt cttaaggtag ttgataatag tttcattgat
     1021 attatcaact gcatctactt ggtcaactcg tactggctta tatcctaatt cctcacagac
     1081 tggggctatc aggtaagaaa aaacctcatc tgctctatct ctagtttctg ttccagattc
     1141 accgatagca gttacaataa aacaaattct ttcagtcata ttttttctcc aatcgttttt
     1201 atttcattat accaaattta gaaaggaact ttatgaacga aatttttaat tttcacgggc
     1261 aggaagtccg tactttgatt attgatgacg aaccttggtt cgttgggaaa gatgttgcag
     1321 acatcttagg atatagcaag gctagaaatg cgattgctct tcatgttgat gaagatgacg
     1381 ccctaaaaca gggcctcaca gataatttag gaagggttca agaaactatc atcatcaacg
     1441 aatctggcct ctactctctc atcttatcca gcaagttgcc tcaagctaag gaattcaagc
     1501 gctgggtgac ttcagaggtc ttgccagcta ttcgcaagca gggcggattt atccgcgagg
     1561 acttggacga ggatgctttc atcgctctat ttactggaca gaagaaattg cgcgagcaac
     1621 aagctagcat gattgaagat atcgactatc taaaaaacga acagccaatt catccaagct
     1681 acgctcagtc gctactgaag aagcgtaagg ctcgggtcgt ggcatgcctg ggtggtattg
     1741 atagtccagc ttatgcggat aagatttttg ctcagtcagt ctttagacaa gctgagattg
     1801 actttaaaga ccacttcaac attagtcgct atgacttgct acccaagaag catgcggatg
     1861 ccgctctagc ttactggatg acttgggaac caagcaccaa tactaagatg aaaatcatga
     1921 aattgaactc atttgacgaa gggtaggagg ggaagaagat ggacaatgtt ctactttcac
     1981 tatctgaatg gattaaatcc attatcaagg acacaatcac tagattggtt gaaatcgaaa
     2041 aagatagtga ccactatcca gagttgatgg atgtgaacac tacctgtgat tttctaggaa
     2101 ttaagtatgc cacattttca gataattatc gttacttaaa gggatttcca aaggaattac
     2161 ctggtaagaa atggtcaaaa agagccatca aagaatggct ctctaatcaa atataataac
     2221 tttactaaaa ggcttctgga caaggtctta gcaaaattat ttgactatat tataacacaa
     2281 aaagaggata aggagataaa aatgtttgaa ccaccgattt tagaccagct gatgggggtt
     2341 ggagccttac tgcttggatt tgcaggggct tggcgtcata tcaaattgca ggaacaacgc
     2401 aaggaagatg aaagacgaga agagcaagaa tttgcgtcta tgattattca aggctataac
     2461 catgcttacg aacgtggtag agaagctgaa cgtcaagaaa tccgcaagaa tattcgtcgt
     2521 ccgttcaagg gctttaccta cgacaatgaa ccgcctcaag gattgcgtcc tgagccgtta
     2581 gctttgccag aacctaaaca gtctgcaatc agatttttgt aatgaggagg tcaggaaatg
     2641 gaagaattga ttgaatggct tgaaaccaaa gaaaaattaa acagggaaat gatttctgac
     2701 gaggaaacat cggactttaa cctacatcta gaaaatgata atgataccat gttcatgatc
     2761 aaggaatact tgaacgatta taacaagcta gccaaagact atcgaattct atcgtctgaa
     2821 aatcgtctac ttaagcttga aaaaatggag ctggaaggca ggcacatcta tgaggatatg
     2881 cggatgaagt accgtgccaa tcgtaggaag tggggtgctc ggtatgtctg aaattaagtg
     2941 gataaaaatc acaaccgata tttttgacga tgaaaagatt tgcctgattg atgccttgcc
     3001 tgatcctgat gccatcttag tgatatggtt caagattttg acacttgctg gaaaacataa
     3061 cagtaatggt ttgttgatga tgactgataa ggttcactat acagatgaaa tgttagctac
     3121 tatttttcgt agaccattga atacagtaag aatggctatt ggagtttttg aacagtttgg
     3181 gatgattgag attatcgatg gtattattag cttgccaaat tgggaaaaac atcaaaacgt
     3241 tgacggaatg gagaaaatca aggaacagac acgtaaccgt gtagccaaat accgtaaaaa
     3301 acagaaaaat cttgctcttg gtaacgttac aggtaacgtt acagtaacgg acggtaacgc
     3361 actagataaa gaattagata aagataaaga attagataaa gatatagaga taaataataa
     3421 taaggtgatg attagttcca gcctctctga aaatttgaaa aatagcggaa tccatctaac
     3481 taataagtca catcaacagt tgcttgaata tgtaggactt gatggaatga gttttgatat
     3541 gttgaaccgt gcagtcgaga aaacttcagg gtcacacaaa cctagtttca attatctaat
     3601 agctattctt gaaagttgga aaaagaaagg cttcacatcg attgaacaag tggatgagga
     3661 cgaccgtaaa tataaagagg gtaaaaacta caatcattca agacaacaaa ataatgaaat
     3721 atcagaacag gaggtcaagg acgaatgggg attttagaac ttatcgagca atttgaagat
     3781 gaattttatc cgataagcga cgaaaagaaa tctttgctta taaaacaacc tctttctact
     3841 gtcactgcct gcttgtctga aatggctagc tggcgtgaat gcggaggtag tctgtcatgg
     3901 tagacaatgt gtttgaggaa attgccttat cttatcacag gagtacagaa caacaagatg
     3961 agttttgcga aaagcataac attcctttga taaagatatt gcggactgag agtgttgtat
     4021 gtcgcatgtg tgaatctgag cggatccatg aggagaatca agtaagagtg aatgaactgg
     4081 ccgacgctga gaatgagcga gagaggaaat actatcttga aaagttctct ctttatgatg
     4141 aggttttgaa aaatgcgact ttggacaatt ttgagacgcc aaccgaaaaa gaagcggaaa
     4201 agctagcttt tgcaaagaag atttgtcgtg agtggtctga gggggttagg aacaacatcg
     4261 tgttacaagg agaagctgga acgggtaaga gccatttggc ttttgctatg gttaaggctc
     4321 tatctgagta cacgaaagag attgctatat ttatcaatgt gactgacttg ttgatgaaga
     4381 ttaaagctga ttttagtcag gaagagtttc tggtcaataa aattgcgagt gccaagtttt
     4441 tggttttgga tgatttgggc atggagaagg atagcgaatg gtcgtttact attctctaca
     4501 atatcctgaa taagcgttca aatacaatca ttaccacaaa tttgacttct gccgatattc
     4561 aaaaaagata tggcagacct ttcatgtcca gactaatgaa gggtgtagat aaagaccatt
     4621 tgatggtttt caatgatttg acaaacaagc gaaagcaata tttttagaat aggggtggct
     4681 gatgtttatt ttaaagcatg ggacaagaga ggataagccg tttctaaggt ctgcaattat
     4741 cagtgtgact ggcttggata tttcgtgttc cgaggagaag aaagccatgc ggtttatttc
     4801 tcgggcggca gccttacagg ttagcaaggc attgagaggt tcctttggga acttctaccc
     4861 tgtcgaggtg gagtgatgtt agagctttat ttcgtctaca acgggcactg caagttttac
     4921 cttggaacgt ttgacaatgt cgatgatctc attgaacaga tggaagatca tcagtgggct
     4981 ttctcggcta tcactcatcc aagatttcag aagcacattg gtcagcggac gacacgattt
     5041 gactacggtt ctaaggattg ttactattta gcgacttttt caggaggaga aaaaaatgat
     5101 tgaacttatt aaagaatttg gaatggctat tctgtggtta tttctcggct atttagtcgg
     5161 ggaacgtgca gcaagaaagg aaaagaaaga tgatcaataa cgttacattt ttagtgggag
     5221 gtaagaaaca tggttggagt aacctatcag gaaattcatc tctttgttga atttttgaaa
     5281 gagcagtacg ggcaaggtcg tccagactat attgaagccc tgaacgattt agacggtctg
     5341 gtggaagtct cctacagaga agctattgaa agatttttag aagatgaagt acgataaaca
     5401 ggctgagatt gacggactga aacgcacgat cgagcaaaac gaagagaaga taatcgagta
     5461 ttcgaagccg tgcgatgcac gcaagagacg gattagagcg ctggagcgcg atttgttgag
     5521 gaaaaagaat aaagaattga gacggaaatt ggaggagttg gaagatgatg gaagagttaa
     5581 agcaaaaagt taatgcagta tacaactgga cggtagaaga cgggaagccg caacctccca
     5641 agcaagattt accacaagtg gtgaaagacc gggcggacta tttttgggaa atggcagaag
     5701 atggtatgac gtttatggga gcgatggaat gcatcttcgc tgatgaaaag cctacagact
     5761 atgatttggg agctactaag ggttggttgc caaaatctaa ggagtttgat gattgggttg
     5821 gctattcgcc aagcatggct caggtagtta ttgcagttta tttgatttac agaggaagcg
     5881 aagatgaaac ttaatgaatt gattaagaaa tataaaaaac ttgagggtgt atggaatgct
     5941 gaaggagcag aactagctcg tcaaattttt ctgcaagact tggaacaact agataaacca
     6001 aaaccagtca aagttccgca gtgtgtggcg gaatatatag aatttaaaaa gaaaaacaat
     6061 tttcatgttt acggtgcaat gagagtaatt gaagatcatt atgataagaa agttcctgat
     6121 tggttttacg aaaataacat cgaaaaattc tgtcttgctt ggcttaacgg ctacgaggtc
     6181 gaaaaagaga agcggtattt tgttaagatt aaagggaata ttaaagaaaa tatgttggtt
     6241 tatggagaac ttttgaaaag gtatttcttt acaaaaagct ttagtttaga cgatgttata
     6301 tattcccaca ctcgtaaaga actagaagac gcaaacttcg gctgggtgtt tgattgccca
     6361 ggtattgaga ttgaggaggt ggaataatgg gagatatacg aatactagac gcttgttgtg
     6421 gcagtcgtat gttttggttt gataaaaatg agagtcatac aactttcatg gacatcagac
     6481 aagaaaaatt tgaaatatat ggaaaaaagg taaacgtaaa ccctgacgtt gtcggagatt
     6541 ttcgtgatat gccattcgat ggtgaaacat ttaatctagt agtatttgat ccgccacatt
     6601 tgaaatgggc aggtcaaaac tcaattatgc gatcacaata tggccagctg gataaagtta
     6661 cctggtcgga agatttagcc aagggttttg aagaatgtat gagagttcta aaagttggag
     6721 gtactctagt ttttaaatgg tctgattgtc aaatcaacgt taaagaagtt cttaaatctg
     6781 taccttttaa accattgttt ggacaacaaa gaggtactac gcattggatg acgtttatga
     6841 aatttgagaa ggtcacagag agaaaggacg gcacgtttgt aaaagaggag gatttggcat
     6901 gataccgaaa tttagagtgt ggcatcatga attaggtaga ctgatgtcag tcaaatgtat
     6961 gttttttcag gatagcgaga ttgaagaatt tgagttaaac gatgctttaa tgaatgatta
     7021 cattacagct tatcctgatg aaatcgaact catgcaatca acaggactca aagacaagaa
     7081 cggcaaggag gtattcatcg gtgacatcgt taaatgtaca agaggatgtc tccatgaagt
     7141 atatttagaa aaagaatacg gtggtacatt cataggtgga atgcctgctg tatacctaaa
     7201 aggacttgga gatggatatg cgtggacgga atatgaggaa atcatcggca acatctacga
     7261 aaatccagag cttttggagg ataaataatg aacccagaaa taattgacaa tataaacaaa
     7321 ccaagccact accaaggtgc aaacggtctt gaggctatcg atgttgtgca taacttcgtt
     7381 gggagccttt ccggagcgtc tgctttcttt tggggcaacg caatcaagta tatgttacgg
     7441 tttcaaaaga aaaatggtct ggaagacctg aagaaagcta gaaagaatct tgactggctg
     7501 attgaggaga tggagcatga gtgaatacgc attgtatgaa ggcgacacct ttattaccat
     7561 ggggacgctt gcggaaatca gcaaagaaac aggaattgct gaacggtgca agtatttcag
     7621 agtttgccat cgaagatatc tgtacgtttt ttgggattga gatccaagaa aggaaataaa
     7681 agtcaatgaa caaaagaatt aagaagaaga tagctaaacg gcagatacaa gaaaagcaag
     7741 aagaattagc caaacaacta cggaagttaa gtcctgaaga aattgaagct atagctaaaa
     7801 tgattaatca ggcagtttct aatattcgca aggctttatc tcaaatattt gataacttgt
     7861 ttacattttt aaaaaacttg gaggtggaaa ttgaaaaaat tgagcgacga agaactcaaa
     7921 acgttagaca gagaactttt caaatttcaa aacattcaac gcacaataga cttaagaagg
     7981 ctagaattag aaaccagaaa cccagatgct caaagtgggc caactgtagg aataagcaaa
     8041 cctaccgaaa ctatcgcaat cagaatcgca gatgatccga ctttaaaatt tcttgaagga
     8101 tttaaaggga ttattaacaa actcttaagc aatctagtcg aggaggacaa agaaattttc
     8161 aatctacgct ggagataccc tcaactgaga tgggaagaaa tagcagaaca gaaattcatg
     8221 agcaaagcta caatctatcg acgtagaagg attatcttag aacagtacgc tataatgaaa
     8281 ggtgagttgt aaataagatt gagacaaaag acatcttgaa gtctcacaaa aaaagggcta
     8341 ttatgatagc atgaacttct gaaaacaaaa acacaaatca catgttggag ttatcctatt
     8401 tttacagaaa agttgtttaa cagaggaaca tcatgagtca gcaaccagct ggctttttgt
     8461 tttgtagaaa ggaggcagtt atggaatttg tatcaccgat aaaagacaat gataatattc
     8521 aagctatgaa agattatctc agagaatgga atgagatgta ttatatgcta ttcatcacag
     8581 gcctgaatac tggcttacga gtcggagata tacttacctt gaaagttaaa gatgttcaag
     8641 gttggcacat caaactgaga gaacggaaga ctggcaagca gataacaaga cggatgacaa
     8701 aagaactcaa gaaagaaatg aggagatatg ttgaaggcaa accatttcat catttcttat
     8761 tcaagagtag gcaaggtcag aataaagcga tcactcgtga gcgagcctat caaatcatac
     8821 atgaagcagc tgaagaactt ggcattgata atgttggcac acatacaatg cgcaagacat
     8881 tcggctataa atattacaac aagacaaagg acgtaggaac attacagaaa atgttcaatc
     8941 actcatcacc tgcaatcacc ctgagataca tagggataga gcaagcagag cttgatgacg
     9001 cactacggaa ctttgtcatt taatttttta gatattactt tcacataatg agttaagcat
     9061 aaactgaaaa aatgaaagtc ttcaaaacct atgattagta agggtttgag atttagagtg
     9121 agtttaacaa aatataagat atgtgaaagt gagggataaa attggtatag ttacaagagg
     9181 tgaaaaatgg taaaagaaca ccgtgatgat tttcttggag aaaaggcctt cgagaaatta
     9241 aataaagata ttgatgccaa tcctgaagtt ggctttgaaa ttgttggata tactcaaaca
     9301 gcatttgtaa atggaatgca tataccgtta acagccatac tagtaaaatg gaataatttt
     9361 tttaaagaat cagaatgaga caaaagacat cttgaagtct cacaaaaaaa ggtttattat
     9421 ggtagcatag atttcttgta tgaggagggg ataggtcaaa ggcctgtccc ttttagcatt
     9481 gagaaaggag gtttgagatg tataacaaac ctatcagacc atccttgaaa tctaagaagt
     9541 gggagaagtt ccgtgatagg ataatgcgta agcatgatta tctttgccaa gaaagtttgc
     9601 gttacggaat ttctgttcaa gcagaaatgg tacatcatat ctttcctgtg tctgaatatc
     9661 ctgagcttga atttgttgaa tggaattgct tgccattgac gaataagaaa cataatacgt
     9721 ttcatgatag agtgaacgat agagtaatca accaaggatt gtattggcag aaaaaaagaa
     9781 aaaaagaatt tttaaatttt ttcaaaaatg aaaaatgaaa atttttaatc ccccccctct
     9841 ttttgaaaaa tcattttggc cagtagggta ccggtgaagg gaactttttc caagtcgggg
     9901 gccttcaaac aaaaaggggg taaaaactaa gcgattttga cgaaaggagg tagtttttgg
     9961 ctaaaccaat tacagcaaag tcgattaagt caaaagtggt caagcagatg aaagacttgg
    10021 gaacttatcg taaagagttc gaaatgatca ttgacatttt tgcaggtatg ttataccagt
    10081 atcagaaact tgctcaagat tatgctgata tgggttatcc agtaacagac acctacgtca
    10141 ataaggctgg tgctgaaaat gagcgcaaag ttccaatctt gacagcgatg gaaattttga
    10201 ggaaagatat actcagctac tctaatcagt tgatgatgaa tcctaagtcg ctcggtgagg
    10261 tagtagaaca agagggtgag tcagttctta ctgaggtact gaagttcaag aacgaaatca
    10321 agaagaagcg agtgactggc aatgggtaat cttgataaag ctaaaaaata cgctcaacac
    10381 gtcttaactc accgagaaga acattgcgaa gaaaacatcc ttgctgctga acgttttttc
    10441 cgtgatttag aaaatccaac ctttgagatg gatgaggata tggtggattt tgttattcac
    10501 tttatcgaga acgtgatagt tcatcagcag ggcgatgata tgtttgcggt atctatccgt
    10561 aacaagccat tgcttttgca accgtggcaa catttcgttg tagttaatct gtttggtttt
    10621 tactacaagg gtacgaatga gcgcaggttc aaagaagcgc ttatcatgct tgctcggaag
    10681 aatgggaaga cctcgtttac tgctgcaatc gcacttgctt atcagatatt agatacagat
    10741 agcggttcca aatgctatat cgttgctaac tcagtcaagc aagcgatgga agcttttggt
    10801 tttttaagat tcaacgttga acgctggaac gataagaaca ttcgtatcaa ggataataac
    10861 caagaacact ctatcaccgc caattttggt gatgaaggtt ctttctttat ccaggcttta
    10921 gcgaatgatg agagccgtct ggactctttg aacggaaatg ttatcatctt ggacgaagct
    10981 cacacaatga ggaacagtaa gaaacatggt cttatgaaaa aaacaatgtc agcatacaga
    11041 aacagtatgc tttttgttat ctcaacggct ggggatattc ctaccgggtt ccttgctaac
    11101 cgtctgaaat actgtcaaaa ggtgctcaag caattagtca ctgatgattc atttttcatc
    11161 ttcatctgca aggctaatca atctgctgat ggggacgtgg tgaactatct ggacgagaat
    11221 atcctcaaga tggctaatcc gtcatggggt gtcacggttt cgctcaaggc tctcaaggaa
    11281 gaagcagagc aggctatgaa tgatcctcag acaagaaatg agtttttcaa taagaccttg
    11341 aatatcttca ctaactctat gaacgcttat tttaatcctg atgagtttat tgcgtcggat
    11401 agctgctacg attggagttt agaagagctg gcacgtttgc ctattcgttg gtatggtggt
    11461 gcggacttgt caagattgca cgacttaaca gctgctgctc tctatggtgt ctatcatgac
    11521 ggtgagaaag acgttgatat ctgtatcaca catgctttct tccctcggat taatgctcag
    11581 aaaaaagcta atgatgacgg gattccactc tttgggtggc agtctgacgg ttggttgacg
    11641 atgagcaaca ctccgaccgt cctctatgat gatatcgtca aatggttcat caagatgagg
    11701 gagaaagggt tcaagattgc tgctgtcgga atggatagga agtttggccg tgagttcttg
    11761 acgaagatga aacaagctcg gttcaagatg attgaccaac ctcagctttt ttatctgaaa
    11821 tcagagggat tcagacggat tgagtttaaa gttaagaata aagaatttta ctatcttcat
    11881 tctgatgctt acgaatactg tgtgagcaat gttagagcaa ttgaaaaggt ggacgatgct
    11941 gtgcaatatg agaaattaga tggagacggt gggactgcaa ggattgactt gtttgatgcc
    12001 agcgtctttt cttgtataca ggctcttgct aatcttggca agggtggtga tgtgatgaga
    12061 ttctttgatt aggtgaatta tgaatgaaat agtattatca gaacatgaaa ttaacttgct
    12121 gatcaacaaa gggcgagtta aagtaatttt aaacggggaa gtagtaacta ttcgtcaaag
    12181 acatatgaaa aatttgatgg ctgaaacagt aaaatgggaa aaacaggtaa ttgatgtcag
    12241 tcagaatatc gtaagaaata aacactttga ttcacttttt caaaatactt ttcgttagaa
    12301 aggaggtgag gaaacatggg tatttttgaa aagttttgga aacgaaacaa gccaagtaag
    12361 tcaatcaaca tgctgagtca ttcagattta gggttgtcaa acctgatgga ctcgtatgta
    12421 cctttggcca gaaatccaga tgtggtgaca gcggttaata agattgctga tttggtctct
    12481 aatatgacca tccacttgat ggagaataca gataaaggtg atatcagaat ccgtgatggg
    12541 cttgctagaa agattgatat caatccgtgt aaacacatga caaggaagtc atggattttc
    12601 aagattgtgc gcgatttgct tttatatggc gatgggaact ctgtcctaca tgtggaatat
    12661 gaacctgtta cggattatat ttctaatcta agaccatttc cgatgagaga ggtttcattc
    12721 caaacagata aggattccta tgtaatctca tttaggggtg aagaatattc ccctgatgaa
    12781 gtagtccact tcgtcatcaa tccagatcca gatattccat acattggtac tggttttagg
    12841 gtgacgttga cagatgtggt tcaaagtttg aacatggcta ccaagactaa aaaaagcttt
    12901 atgaacggga agaatattcc tagtcttatc gttaaagtag actcgtctag tgctgaacta
    12961 gattcggagc aagggcgtga gcgtatcgct gagaagtatt tgagtactag cagggttggc
    13021 gctccatgga ttgttccaga ggcattgctg gacattcagc aggtaaaacc gcttagtcta
    13081 acggacatcg ctttaaacga gtctgtggaa ttggataaaa gaacagttgc aggtctatta
    13141 ggagtacctg cttttatttt gggtgtagga gagttcaaca agacagagta taacaacttt
    13201 gtaaatacga ctgtcatgag tattgctacc actattactc aaacactaac cagggactta
    13261 cttttgtcta gcaatcgtta cttcaagcta aatcctcgtt cactcttctc ttacaacatt
    13321 acggagttgt ctgctgttgc tcaacaaatg gcaaacagtg ctgcaatgcg tcgtaatgag
    13381 tggagggatt ggctagggat ggctcctgat cctgagatgg aagagttgat tgttcttgaa
    13441 aactttctcc ctcaggagaa actaggagat caaaataaac tgaagggagg tgaggaagag
    13501 aatgcaaaag cgaaatagct atcgtgccac tcaatttcaa acgagagaag aagaatctgg
    13561 agacttggtg ttgagtggct attttataaa atttgatgaa gagacggagt tgtggcgtgg
    13621 ttatcatgaa gtaatcaagc gtgctggtgt tgagaaagct gtcacagacg ctgatatcag
    13681 agctttattt aaccatgatg atagccttgt tctcggtcga acaggtaacg gaactctgac
    13741 actgggtgtt gatgatgttg gtctttttgg agatatcatc attaacaagg atgatcctca
    13801 agctgttggg gcctatgccc gtgtcaagcg tggagatgtt atagggtgta gctttggctt
    13861 tataccggta aaaatcgaaa cggaagaacg tgaagatggt tcatatttgg acactgtctt
    13921 agagctagaa atctttgaag tgagtccatg tactttccca gcctatccac aaacggaaat
    13981 tgctgcacga caaaaagact tcgaaagtca gaagcgtgct aatcgtgaag cgctagacaa
    14041 gcgcaagaaa gaaattaagg agaaatttaa gctatgaata aggcattaat ctttggtgct
    14101 cgtatgcgag caaaagcaac taaggtagtt gagttggaag aaactatcga agaattgaac
    14161 aaacgttcgg ttgttgagtt agaaaagttg gatcgtgcta aaaatgatga agaagtttca
    14221 gcagttgaaa agactgtaga cggtcttcaa agggaaattg aagaaaaaga agctgaaaaa
    14281 gtacagcttg aaaatgagat cgatgagtta gataaacaaa tcaaagagca aaatcgtaaa
    14341 gcaccaactt acccaagtca agaaaaacgt ggaggacaga aattggaaca acgtgacgca
    14401 atcgctaaat acattcgtac tggtcaaact cgtgacattg taggcttgaa aactactgat
    14461 tcaggaagcg cagctttaat ccctactgaa gttttgaaac ctcattttgt taataaaaca
    14521 cgtaatccac ttttggatct tgtggaacgt gtgaaagtta atagtggatc tggtaaatat
    14581 ccagttatca agaaaacgga tggtgtaatg gtttcaacag atgaattgaa atcaaatcca
    14641 gaactcggaa aaccagcaat cagcgagatt gattattcaa tcaagactta ccgtggatat
    14701 gtccctgtgt cacaagaaat gattgacgac gcagactatg acatcatgtc cattgttgaa
    14761 gacgaagtgt tcaatcaagg tgaaaacact gaattgtcat tagttacagc tgtcctcaaa
    14821 acagctaccc aagcagatgc ggctggattt gatggtatta aagatatcta caacaagaag
    14881 cttaaatcaa tttataaagc aagcatcgtt gtaactaagt caatgtttgc tgcgcttgac
    14941 aaggtgaagg acaaagatgg gcgctacatg cttcaaactg atgtggcttc acctactggc
    15001 tattcatttg gtgggaaaac aatctacaaa gtagatgata cagtgtttgg aaacgaagga
    15061 gatatgaaat tcttcatcgg agatgttact gagttcgtca aagagtttga ccgttctcaa
    15121 gtatccgtta aatgggtgaa caatgacatt tacggacaat tgcttgggct ttttatccgt
    15181 ttggatatta agaaagcaga tgaagaagct ggattcttcg gaacatatac tgatgttgta
    15241 gcttaaggag gtagcgtatg agctataaag taatccgtcc ttttaaggac ttacgagacc
    15301 caaagaaaca ttgctataag gttggggata attaccctag agttaagcac aaacttgata
    15361 aagagtttgc ggagactctg ctaaatgggg caaatagtgc aggatctatc tttttgatga
    15421 atgtagaaga taaggatatg tcgaaagatg acacattaac tacagaagta gaaaaggaag
    15481 aaacaaaggt agtagatggg aaacctgaag agggagacga ggagtagtta tggacaatgt
    15541 tcaattatta gaattgctta agctaaaatt gggtatagca acaaaactac gcgataagtc
    15601 cttggagaaa atcatcgaag ctgtcaaaac agaactagaa gataacttag gagttctact
    15661 tgacttagac agctcagaag accaaatgtt tgtagttgat tttgcagcct ttcgctatga
    15721 gggtggggtg gatatgccac gtcaccttca gtggcgactg cataatttac agatagcatc
    15781 aaagaaagag gtcaagaatg tggaatcatg aaatcacact gatctctaag gaagtcacag
    15841 gtaaggataa actactacaa ccaatctctg aagatgttga agttactctc ttatgtcgca
    15901 aaaagagggt cactcgctct gaattttatc aggcgaatca ggtagggctt aaaccgagct
    15961 tggtcgttga gattcgaaat tttgagtatg agaatcaaga gcttgcgaag ttcgaaggca
    16021 agcaatatca tatcttgaaa acctatccta ttgattctga aattttagag ttgactttgt
    16081 cagaggtgtt gaaatgagct taacaagtga tttagcgaat gaaattgcaa aggcaatggc
    16141 agagtactct gctgaggtag aagataagat tgacctgatt gctgaggacg ttgtaaacga
    16201 agccgttacg gaattaaaag cgactagtcc aaaacgtcat ggaaagtatg ctagaaattg
    16261 gcgcttcaag aaaaatgcta aagggtcata cgtcatctac aacgcagctc caacctatcg
    16321 tttaactcac ttactagaaa atgggcatgt tttgagaaat ggcggtcgta gtcgggcatt
    16381 tccacatatt aaacctgttg aggagaaagt taaagaaaac tttgagaagc gaatcaagga
    16441 gattggaaaa tgaagctatc agactttgca gctattttgg aacaggtaaa cttgcctgtc
    16501 acttatcgag cgtttaaaac tgggaacgct ccagacctac cttatctggt ctattatgaa
    16561 tcaattccag ccatcaatgc agctgacaac acggttaatc atcagattaa gagcgtgaca
    16621 gtagagctag cttttgagag taaggatgaa gatttggaag aacgtctgga agagctgtgg
    16681 acaacccacg agctcttttt cgatgttcaa gaagaaacat ttatcgagac tgaaagactt
    16741 tatgtcaagt cttatacggt ctatctatac caaggaggaa tgacatgact caagaaaata
    16801 aagtaacctt tggtttaaaa aatgttcacg ttgcgccaat taaatcaatt ggtgcagatg
    16861 gagtgattgc ttacgatgaa attttccgct ttcctggagc aatggaattg acattggatc
    16921 caaggggtga atcaacacca atcaaagcag acgatatcga ttatcacttc atgaactcaa
    16981 acgaagggta tgaagggaaa ttcaaaatct ctcacattat tgaaatgttt gcgactaaga
    17041 ttttgggtga aatcaaagat gctcagacgg gtgttttgac tgaaaaagct gatgcagaat
    17101 ccacatcatt tgccttgatg tttgaatttt caggggacaa gaataaaaca cgtcacgtcc
    17161 tttactactg ttcagcgagc cgtccaggca atggttcaaa aaccaaaaat ggtacaaacg
    17221 tcaacgagcg tgaacttggc tttaaagcaa gtcctcgccc tctggattca gttattaaac
    17281 gttctatcac atcagctgat aacaaggaaa tttatgacaa ctggtttaag aaagtgtatg
    17341 aacctactgc ggtggcagct taaggagaaa atctatgcgt aaaatcgttt gggttggcga
    17401 tcaggagtat gagttgggga ccaatggcta tactcctatc gcctacaagc aacaatttgg
    17461 gaaagattat tttcaagatt tgttttcaat gttgaaaaat caatcattca tgaatgaatt
    17521 gaacaagctg gaaactcaca aagagttgac tgcaactgat attgacattt cgatgctgtc
    17581 agattttgat atgacatttt tcaaccgtct tttttggacc tttgctaaat ctgcaaatcc
    17641 tcacatcaag ccttatgaac aattcttcat ggaaatggaa gtctttccga ttcaggaagt
    17701 tgggcctgta ttgatggaaa tgctgaatgc gagcatgacg acaaaaaagc accagatgaa
    17761 tcagaatcag ctagcgaaga aatcttcaca gtagaatcct acttatcttg ctgtaaagaa
    17821 actggtcttt ctattgatga tctaaagcac atttcaatcg gaatggctct ggattatcag
    17881 acggattatg tgaatctacg gagcgaggat aagggtggcg aacggaaagc cacgcaagct
    17941 gattttgaca gtttttaaag aaaaaatgag tgctgagaga gtgattctga ggtcaagttc
    18001 cttgccctga ctgcattatc agtcgtagaa gttctctcag cgcttttcta ttttttgaga
    18061 aaggaggaaa tatggcagga aatatcaaag gtatcaaaat tgaaatcgat ggcgacacgc
    18121 aacccttgca gaaggcgctg aaaaatgtca ataaggccgc tactgatgca agtcaggagt
    18181 tgagacagat tgacaaggcc ttgaaatttg atacagggaa cgtaacgctc ctgactcaga
    18241 aacaagaggt cttacaaaag caagtttcga cgactaagga gaaactggaa accttgagac
    18301 aagctcagtc tcaggtggaa cagcagttca aaaatggtga tatcggtgct gatcagtacc
    18361 gtgcctttca acgcgaagtc gaagttactc aaaacgtcct aaaaggatat gagggtaagc
    18421 ttgcaaatgt gaatcaggcg cttaatgaga atgggagtgc tactcagaac aacaagaacc
    18481 aattaaaaga gttgcaaaat gagcagagtc aacttgcttc agagatggta aaggtgacaa
    18541 gctcattcaa actgcaagaa agcgctttgg gttcaaatgc tagtgaagcc gagagaaatg
    18601 ctcttgctca gaaaaagatt ggtgctcagt ctgagattgt aagtaaacag atttcaaatc
    18661 tagaacagca attggaaatc actaaaaaag aatttggtga gaactccaca caagccaaca
    18721 ggatggaagc tgagctaaat caggctaaga cagcattcaa tcatctcaat gatgagatga
    18781 aaggaacaaa gtctgctgct gatagcactc aagaaagttt aagtgaaatc tcaagaaatt
    18841 taagagcaga actacttcaa cagtttagtg agaagttgag tgctatttca gaaaagcttg
    18901 tggaagtagg aaaagaagcg ttagaagcag ctgctcaaat gcaagctagt aatgctcaat
    18961 ttactaccgt tttcggagat atggaaaccc aagcaagaga agcgttgaat gctattggtc
    19021 aggaaatgga tattgtccca gagcgattgc aaggatcatt cactcagatg gcttcatttg
    19081 caaaaacttc aggattggat acagcagaag ctttggatct tacttctcgt gcaactaggg
    19141 cagcagcaga cggtgcagcc ttctatgaca aatctattga gagcgtgaca gagagcttac
    19201 aatctttttt gaagggaaac tttgctaacg atgccgctct tggaatctct gcgacagaga
    19261 caactaggaa tgcagctgca aataaattgt acggaaagtc attcaaggac ttgagcgaag
    19321 cgcagaagca attgacattg cttcagatgg tcgaagacgg aaataaactc tcaggagctc
    19381 ttggacaggc tgcaagagaa tcagacggcc tagaaaacgt tatggggaat ctgaaacaag
    19441 ctgggaccaa tgcattatct gctattggtc aacctcttct ggaaatgatg atccctgttt
    19501 tccaaacctt ggcaacgatt gtgaaaggtg tagctgagct gttcagttcc ttacctgctc
    19561 cagtaaaaga ttttgttgtt attttaggaa cagttgtgac tgctgtaggg gtcatagccc
    19621 ccatattctt atcgttgcaa gccctagctg agtttttaaa aatatctatt ggagaaatga
    19681 taattgccgc attgccaatt attggaacag ctattgcaat tgctgctgca gttgctgcaa
    19741 ttattgttat tgtaaaatac ctctgggaaa ctaacgaagg ttttcgagat gcggtcacga
    19801 ccgtttggaa tgcgattctt gaagttatca atgcagtcgt atcagagatt tctaatttta
    19861 tcatgagtat ctttggaacg gttgttgctt ggtggacgga gaaccaggaa cttattcgag
    19921 caagtgctga gactgtctgg aatgctatct atacggttat aagcacaatt ctggaaattt
    19981 taggtccact cattcaagct ggttgggata atatccaact tgtcattaca acagcttggg
    20041 aaatcatcaa gaccgttgtt gagactgcaa tcaatgttgt ccttggtatt atccaagcag
    20101 ttatgcagat cattactggt gattggtcag gcgcttggga aactatcaag ggagtattct
    20161 ctactgtatg gcaagctatt caaagcattg ttcagactat tttttcagct attcagagtt
    20221 acatttcaaa tatcctcaac ggtatttcag gaactgtatc aaatgtctgg aacggcatca
    20281 aggatactgt atcaaatgtg ttaaatgcta tatctagtac tgtatcaagt gtttgggaag
    20341 gtatcaagag taccatttca ggtgctatca atggggcaaa agatgctgta tcttcagcta
    20401 ttgaagctat caagggattg ttcaacttca gcattagctg gccacatatc ccactacctc
    20461 acttctatgt aagtggttca gccaatccat tagattggtt gagtcagggt gttccaagta
    20521 ttggaattga atggtatgcc aagggcggga tcatgacgaa accgaccatt tttggaatga
    20581 atggcaatag ccttatggtt ggtggcgaag ctgggaatga agcagtatta ccgcttaatg
    20641 acaaaacact tggtgctatc ggtcgaggta tagctcagac tatgggtgga acttcaccga
    20701 caatcaacat tactattact ggtaacaccg tcagagaaga agctgacatc attcggattg
    20761 ctgatgaggt ggctcagcgt attgctgacg agttgcaacg taagacacaa ttgagaggag
    20821 ggtttacatg ataaagcata atgagcttgt gattgacggt gtgagaacat cgtcttttcc
    20881 gtttaaagtc attgtccatg attctccttc aattgctctg ggagagagca agacagctct
    20941 tttggagcat ggtggtatca gtggagcaat cgttcagacg aacaaacata gggaattggt
    21001 caagaaaacc tatacgattt acttggtcaa acctactgaa gaacagatga accaatttat
    21061 gagtctgttt atccgtgaaa agttctggct agagagtgag cgagtcaaaa caactcgtct
    21121 ttggtgctat aaggtcaatg tgagcgacct tgaagaagtg caacctggtc tttacatgac
    21181 caaagcaacc ttcacttgtc accctaccaa acacttcaaa gtcattgata cacagagatt
    21241 gacaagaagt gggactctga atgtgcaagg ttctgctctt gcttttccta aaatcacaat
    21301 cgttggtcag agcgcttctg aaacttcgtt tacaatcgct ggtcaggtca tccgtcttga
    21361 acgactcact gagtcacttg tgatggtcaa taatcctgac aatccaagtt ttaaaacaac
    21421 aacagggaag ccagtcaaat ggtcagggga ttttatcaca gttgatccag cgaaagtgaa
    21481 gaatattggg gttgttttgg gtccaggtat tcaatcactt gaaattgaga cggtttgggg
    21541 gtgggcataa ttgctttatc tacttaataa agatgtgaga accgttcggt ggaacgggga
    21601 gccacttcat gaagcgactt cggcgattgt taaagagatc atgaatggtg atttcacctt
    21661 aactgtgaaa tatcccattt ccgactctgg tatttatcag ctcatccaag aagatatgtt
    21721 gataaaagcg ccgactcctg ttcttggtgc gcagctattt cgcattaaga aacctgttga
    21781 atacaatgat catctggaaa tcacagccta tcacatttca gacgatgtga tgcaacgttc
    21841 tatcacacca gtaagtgtga ctagtcagag ctgtggcatg actctttctc gcatgattca
    21901 aaacaccaaa actgctttgg gagatttttc tttcaatagc gatatccagg accgtaggac
    21961 cttcaatacg actgaaacag aaaccctata ctctatattg ctggacggta agcacagcat
    22021 tgttggtaca tgggaaggcg agctggttcg tgataacttt gcgatgactg tcaagaagag
    22081 tcgtggggag aatcgtggtg ttgttattac aacgcacaaa aatctgaaga actaccaacg
    22141 cacaaaaaac agtcagaatg ttgtcacaag aattcacgca aagtcgactt ttaaacctga
    22201 aggtgctgaa aaagaaacga ctatcagagt gactgttgat agtcctctta tcaactcata
    22261 cccttatatc aatgaaaaag agtatgagaa caacaatgct aaaactgttg aagagttgca
    22321 gaagtgggca cagtctaagt tctcaaatga gggaattgac aaggtctctg atgctatcaa
    22381 gattgaagct tatgaacttg atgggcaagt ggtccatatg ggcgacacgg tcaatctcaa
    22441 gagctggaaa cataatgtcg atgcattcaa gaaagctatt gcttatgagt tcgatgcctt
    22501 aaaagaagaa tacatctctc tgactttcga tgataaggca ggtattggtg gttctagagc
    22561 ttctggtggc ctatctagcg cagccgatgc aattcttgga gtgacagaat ctgcacaaga
    22621 aatcgccctt gaaaaggctc ttcaaaatgc tgacttagac tttgatcata aggctggatt
    22681 gcttagacag gaaatttctg acgatattga actggccaaa gccagagctg aagaagtcaa
    22741 gagagaactg tctgacacta tcaatcagcg atttaatagc tttgacaacg ggccattgaa
    22801 agaaactaag cgcaaggctg aggaagcttt gcgaaatgct ggcgcaagta ccctgcttgc
    22861 acaggaagct aagcggattg ggctggattc tgt
//
LOCUS       DAVLFB010000026        22850 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLFB010000026 DAVLFB010000000
VERSION     DAVLFB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22850
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.026"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..612
                     /locus_tag="VXD58_000901"
     CDS             <1..612
                     /locus_tag="VXD58_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0675632.1"
                     /translation="GNSNTRVLLMTHDKQTIYDFDIFLKEIMESCKEEEGGQKSKYKK
                     LELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMRKILEAYGSFNYK
                     KGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTLSIDFFDTISDEE
                     RKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            785..2083
                     /gene="purB"
                     /locus_tag="VXD58_000902"
     CDS             785..2083
                     /gene="purB"
                     /locus_tag="VXD58_000902"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV0675633.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(2138..6076)
                     /gene="strH"
                     /locus_tag="VXD58_000903"
     CDS             complement(2138..6076)
                     /gene="strH"
                     /locus_tag="VXD58_000903"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0675634.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(6391..7107)
                     /locus_tag="VXD58_000904"
     CDS             complement(6391..7107)
                     /locus_tag="VXD58_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0675635.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            7455..9242
                     /locus_tag="VXD58_000905"
     CDS             7455..9242
                     /locus_tag="VXD58_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV0675636.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            9239..9715
                     /locus_tag="VXD58_000906"
     CDS             9239..9715
                     /locus_tag="VXD58_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV0675637.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            9743..10648
                     /locus_tag="VXD58_000907"
     CDS             9743..10648
                     /locus_tag="VXD58_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0675638.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            10635..11450
                     /locus_tag="VXD58_000908"
     CDS             10635..11450
                     /locus_tag="VXD58_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0675639.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALTLSALGHFALGM"
     gene            11457..11861
                     /locus_tag="VXD58_000909"
     CDS             11457..11861
                     /locus_tag="VXD58_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0675640.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            12159..13325
                     /locus_tag="VXD58_000910"
     CDS             12159..13325
                     /locus_tag="VXD58_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV0675641.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKKLVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALDCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            13441..14478
                     /locus_tag="VXD58_000911"
     CDS             13441..14478
                     /locus_tag="VXD58_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV0675642.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            14685..15539
                     /locus_tag="VXD58_000912"
     CDS             14685..15539
                     /locus_tag="VXD58_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0675643.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            15689..15946
                     /locus_tag="VXD58_000913"
     CDS             15689..15946
                     /locus_tag="VXD58_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV0675644.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADLVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(15988..16305)
                     /locus_tag="VXD58_000914"
     CDS             complement(15988..16305)
                     /locus_tag="VXD58_000914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675645.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEEYKSPHNKTHTIKVFMYCPPKVRQKKSNFWGQYISIPDTMRFFNFKDFLQLLLTHI
                     KLF"
     gene            complement(16377..16655)
                     /locus_tag="VXD58_000915"
     CDS             complement(16377..16655)
                     /locus_tag="VXD58_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0675646.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNA"
     gene            16877..17641
                     /locus_tag="VXD58_000916"
     CDS             16877..17641
                     /locus_tag="VXD58_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV0675647.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(17881..18078)
                     /locus_tag="VXD58_000917"
     CDS             complement(17881..18078)
                     /locus_tag="VXD58_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675648.1"
                     /translation="MKTKASIRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADKTDEVS
                     SKHCFEVADRTDEVSNIYTAR"
     gene            18296..19675
                     /locus_tag="VXD58_000918"
     CDS             18296..19675
                     /locus_tag="VXD58_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0675649.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            19689..20354
                     /locus_tag="VXD58_000919"
     CDS             19689..20354
                     /locus_tag="VXD58_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV0675650.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            20468..20563
                     /locus_tag="VXD58_000920"
                     /pseudo
     CDS             20468..20563
                     /locus_tag="VXD58_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            20754..21293
                     /locus_tag="VXD58_000921"
     CDS             20754..21293
                     /locus_tag="VXD58_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0675651.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            21360..21770
                     /locus_tag="VXD58_000922"
     CDS             21360..21770
                     /locus_tag="VXD58_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV0675652.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLY
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            21886..>22850
                     /gene="pavB"
                     /locus_tag="VXD58_000923"
     CDS             21886..>22850
                     /gene="pavB"
                     /locus_tag="VXD58_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV0675653.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLE"
BASE COUNT         6310 a         4300 c         5113 g         7127 t
ORIGIN      
        1 gggaatagta atactagagt tttactaatg acccatgata aacaaacaat atatgacttt
       61 gatatctttt tgaaagagat aatggaatcc tgtaaagaag aggaaggtgg acaaaaatct
      121 aaatacaaga agttagaatt ggtttctggt aaattacaag aattcaaaac tagtactcac
      181 gattatacag aactacttga aatagtcttc ggttatgcat tgggaaatag tacacctact
      241 tcggaaagtt ttgttgggaa tgcaatgaga aaaattttag aagcctatgg tagttttaat
      301 tataagaagg gaattgccga gctaacaaca gatcctttaa ttgttgaaaa aattgataaa
      361 gaatacagaa cttattttga gaatcttatg tatagactgg ttttaaacgg tgaaagtcat
      421 tttaaggatc cggttaaaac tttgagtatt gatttctttg ataccatttc tgatgaagaa
      481 cgaaaaaaaa cagctagaga tttactagtt ttgctttatc ttttagatga tttacatgtt
      541 ttaaaacatt tagagggtgt atcaaatgcg gaaaatagac ttgaacaatg gaagtgtgag
      601 attttagaat aagagaatca taccatttga cttattgcaa tggttgaagt ttgcatgata
      661 gctgattaga gtaatcaaag gattattact tcctaatttt attttctaca aaccctaatt
      721 tagcatctaa taatgcaaag tttcctgagc ctcatagata aaaaagattt ttaaggagaa
      781 aaacatgatc aaccgttact ctcgccctga gatggcgaat atttggagtg aagaaaataa
      841 ataccgtgct tggcttgagg tggaaatcct ctctgacgag gcatgggctg agttggggga
      901 aatccctaag gaagatgtgg ctttgattcg caagaaggcg gactttgaca tcgaccgtat
      961 tttggaaatt gagcaggaga cgcgccacga tgtggtggct ttcacgcgtg cggtttctga
     1021 gactcttggt gaagagcgca agtgggttca ctatgggtta acttctaccg acgtggtgga
     1081 tactgcctat ggttacctct acaagcaggc caacgacatc atccgtcgtg accttgaaaa
     1141 cttcactaat atcatcgctg acaaggccaa ggagcacaag ttcaccatca tgatggggcg
     1201 tactcatggt gtgcacgctg agccgacaac ttttggtctt aaattggcaa cttggtatag
     1261 cgaaatgaaa cgcaatatcg agcgcttcga gcatgcggct gctggtgtag aagctggtaa
     1321 gatttctggt gcggttggga actttgccaa tatcccacca tttgtagagg agtatgtctg
     1381 cgataaactt ggcatccgtg cccaagaaat ctctacacaa gtccttcctc gtgaccttca
     1441 cgctgagtac tttgcggttc ttgccagcat tgcgacttca atcgaacgta tggcgactga
     1501 gattcgtggt ctacaaaaat ctgagcaacg cgaagtagaa gagttctttg ctaaagggca
     1561 aaaagggtct tcagcaatgc ctcacaaacg caacccaatc ggttctgaaa atatgactgg
     1621 tctggcgcgt gtcattcgtg gtcacatgat tacggcttat gaaaacgtcg ctctctggca
     1681 cgaacgcgat atttctcact catcagctga gcgtatcatc acaccagata cgaccatttt
     1741 gattgactac atgctcaacc gttttggaaa tatcgtcaag aacttgacag tcttcccaga
     1801 aaatatgatc cgaaacatga actcgacttt tggtcttatc tttagccaac gggctatgtt
     1861 gacattgatt gaaaaaggca tgacccgtga gcaagcctat gacttggtgc aaccaaaaac
     1921 agcctactct tgggacaacc aagtagactt taaaccactt cttgaggcag attcagaagt
     1981 aacatcacgt ctcacacaag aagaaatcaa tgaaatcttc aacccaattt attacaccaa
     2041 acgagtggat gatatctttg aacgtcttgg actaggtgat taattaaaaa ataaacagcg
     2101 agcttcaatc tcgctgttta ttttttatcg aaaagactta gtcttctttt cttttagtga
     2161 gtccataggc tgctagtgtg gacatgagtc ctgcgactac tagtcctgca gaatcgtgag
     2221 ttcctgtttc aggaagtttt ttctctgtta ccacaggagc tggatcttga ggaagagctt
     2281 tgctttcctc agcaggagca gttgatggag ctggttggct tgggatttct agtcttggtt
     2341 tttcttcagc aatagcggct tgtccgtttt catcgcctac atgtgttacc atagttccga
     2401 cttcgactat ttgagtaacg gcttcctgtg ctacgacact atttacaagt gttttcactt
     2461 ccttaccatc ggcagaagtg ctcacagagt agaagttgct acgatgtcca ttgacgccct
     2521 tagtaatgac ttgtgttttt cctttgagta agagtggatt ttcacgagtc actgtggtaa
     2581 atggaatttc ttctttttgg atatccagtc taggttttac ctcagtagtt ggtgcaagac
     2641 cactttcatc acccttgtga gttacaggag cgccaacttc aaccacttgg tttataactt
     2701 ctttggttac ctggctatca aggactgttt ctgttgtttt tccattttca gtgagtacag
     2761 agatgtaatg agttcgttca cctttgactc ctgctgtgat aatattttcc tgaccagctg
     2821 ggaggttagg attttctttc ttgataactt caaatggaat ttcttcagtt cttgtgatga
     2881 gttctggtct ggtttcaaca ttggcagcca cttcattttc atccaggctt cctgaatgag
     2941 ttgcagctgg tttgaggcct tgaagagcgg cttttaggtt ggctacaagt gtgtcaagct
     3001 cagcttgttt attacggttg aggttgtaat ttagagctgt tttagctgcg tcaagggcct
     3061 caagactttc tttactatat ccttctaagt ttgtaggaat tttagctaat tcttcgcgga
     3121 gagcattata attagcacgg aagtagtctt tgttgtggtc tgcaaaggca gtcatgagtt
     3181 caaagatttc ctcttccttg tactcagcgc ttggtctatc tgcccagatt gaaagcatac
     3241 ttccgactgt tggaagatct acttcaggat atttggtaga agctagttga ttgaatggaa
     3301 ctttaccaga attttcaact gcttttgaaa gagggtaagc ttcatcttgt ttgtgattgc
     3361 ctataacata ataccagtca ccgttggtat tcaagaattt atagcctttg cttgctaggt
     3421 attgaggtga tgcgaggtta tatccccacc agcctttaga ccagtaagaa atcaagacat
     3481 ctttgtcaaa ctgaacatcg tccttgtctt catagtagaa gccatcgttg aaggccattg
     3541 gttgaagccc tctttctttg gccatagctg cgagggtgtt ggcatattcg gcaaatttgc
     3601 catagagttg ataccacttg aggtagtacc agccttgggc actagtcgca tcgttggcgt
     3661 attcgtcagt accaaagttg aaaatctttg ttttacctgc aaagaagtcc atgtatttac
     3721 cgatgagggc ttttacaaag ttcatcgctt cttcgttttt caagtccata gttgtttttg
     3781 aaactttatc aaagtgggct tgaggatttt taatacctaa tttttccatg gcaaccagca
     3841 tagcatccat gtgacctgga ctgttaatag ctgggatgag accaatgtcc ttagatttag
     3901 cgtattcaat tagctctgtc acttctgcct gtgttagtgc agtaccgttt ggatcgtcgt
     3961 agtaagcttt agttccttcg ataatagctt ttttaacgtc atcactagca taggtttttc
     4021 cgttggcagt aatggtcata tcattgagta gaaagcgaag tccgtcattt cctagaagga
     4081 gatggacatc agaatatccg agctcactgg ccttgtctac gatgcgtttg agctggtcca
     4141 gagtaaagta tttgcgtcca gcatcgattg agattacctt gtttttggca agtttttcaa
     4201 cctcacgttt agcttcttct tctttttgag cttcaggcgt gagggtcaag ttgttgacag
     4261 tttcttgaag tttagcaatg gcttgatcaa tcgtatcttg ttgggcacgg ctaaggttgc
     4321 tatcgagaga gcgaatagct ttttcagctt cttttacggc cgtgacgctt tctgcagtat
     4381 aacggttcag gtcttttggt acctcgttaa gtgcttgctc tgcagattca taatcagctg
     4441 cgaagtattc agcgttggca tttgcaaaat gacgcatgag tttgaagagg cgtgatggtg
     4501 aataacgtgc agatggagtg tcagcccaag cagctaccat accaccgatg attgggatat
     4561 cagctccttc tgtttttggt acagaagtga ttggtgtgtt tttaatacca ttgagcccct
     4621 gatcgagatt gtaccagcct tggccatcag cgtttcgtcc aagaacgtag taccaagcat
     4681 cattggtatt aaggatttgg tgaccttttt cagctagtag tttagaagaa gcgacatcgt
     4741 agcctcccca accaccagtc cacatagaaa cgatgatgtc tttgtcaaaa ctaccaaagc
     4801 ttgtgtcgct attgtagtag ataccgtcgt taaaggccat tggtttgaga ccgtgcgatt
     4861 ttacaatacg agcgaggtca ttggcgtagg caataaattt ttcatagcct tttacagggt
     4921 agccttcgtt tggatagtat ttatcagctt gaagcacact ccaaccttta gcatctgtcg
     4981 catcattggc atattcatca agtccgatgt tgaagatttc agtctttttc gcgaaataag
     5041 cagcatactt gtcgataagg gcttttgtaa aagcgacagc ttgttcgttg tcaagatcga
     5101 cagtacgggc tgatttcttc ccaaaatagc taaagttagg gttttggatt cccaattctt
     5161 tcatggcatt gagaatcgca tccatgtgtc caggactatt tactgtcgga atgagaccga
     5221 tacctttatc tttggcatag ttaatcagat ctgtcatttg actttctgtt aagtgattgc
     5281 cgtttggatc gttgtaataa tcatttgtac ctttttcaat ggcgcgtttg acatcgtcac
     5341 tggcataggt cttgccgtta gctgtgatgc tcatatcgtc caacatgaaa cggagtccat
     5401 catttccgac taataggtgt aaatcagtgt agccataatg tttcgcttta tcgatgattt
     5461 ccttgagctg ttctggtgag aaatatttac gtccagcatc aatagaaaca attttctttt
     5521 tcgctagttt ttcatttaca gttgcagcac gttcctttcc tgtctctgtt tccggtttgt
     5581 cagcctctgc tttcgcttca tcttttttag ctggtttatc cttgtcagtc ttgtctgtat
     5641 ttgactcttt agaatcaacc tctttcgctt cttccttttt agggctagct tcttcttcct
     5701 ttttattagc agtttctttt tcagcagaag ttggagttac cacctctgct ttatcactag
     5761 gagttgaact aacttcctct tgtggttttt cttctgtttt tggaagacta gctaccttat
     5821 cagtagctgg agtttctgtt tctacagttt ttggagcttc tggttgaagc tctgctttag
     5881 gtgtttcctc agtccgattt tcggatgatt gaggggaatc agaaaccgta tggatggtcg
     5941 gttggttttc tgtagtagta ggagtaactc catcggctgc aacagtctgt gcttggaagg
     6001 caaatccaat tagaacagaa gctgctccta cagcgtattt acgaatagaa aaacgctgtt
     6061 gtttttcatg tttcattgca aaacctcctg attgcattgt tatattgata gcgattatat
     6121 aaatcaacgc ctttatttta tttcttatat taacgagagt caagaggaga tgacaaaaaa
     6181 ctataataag tataaaaaaa tataaaattt aaacttaaga tttcagattg gtcgaaaaaa
     6241 tacatatata tatctagtat aatttttggt tctatttcta taaaatattc cacaaattat
     6301 agaatcttcc aaaaataggt aagcgctacc tttttggtgt ggtataataa gcatagaaaa
     6361 agcccgagcg attaggctca ggctttcttc ttagtgatca cggtcacatg agatgaattt
     6421 aatcttgtag taatcagatc gtttgtaagt ttcactgtat tctaaaactt ggccagttga
     6481 ttcgagtttg gtgattttag tttgtaggac agtagggaat tgttcatcga ctccgaggac
     6541 tgaagccgca tgttctggag ttggaaagac tatttcgttg atttcttcaa agtgttcatc
     6601 attcatgtga atgtggtagt ctaacttgaa acgattatag atagaactat agtattcaag
     6661 gtttggataa tttgcgttga tatattgttc tgggatgtag gatgtatggt agatataaac
     6721 gacaccgttt gattcgcgga tacgttcaat cttgtagtag aattgatcgc cgcgtagacc
     6781 caatttttcc aagtaaacaa gcttgtttcc gcgttcaatt gaaagaacag ttaccttatc
     6841 atctttagca ttgaagagtt caatatctga aaactctaca agcttgtgtt tgcgtgcacg
     6901 tgaaacgaag gttccttttc cttgttggcg gacaatatag ccatctttgg caaggtcgtt
     6961 taaggcgcga acaactgtga tagagctgac atcgtacatt gaaatgagtt ctgcttcagt
     7021 gtaaaattta tctccactgc taaactgccc agagatgatt ttatttttta attcgtcttt
     7081 tatgtattga tatttgggaa ttgccatatt tttacctcta ttttccttct ccatagaaaa
     7141 ttttaccata aaagcgaaaa aaatagtagt cttttgaata aaaaattaga ataatagttt
     7201 gaaaaattaa gcgatatagg gattgctcat attttatcaa tgcattctat acaaaattat
     7261 tttaggtcct atattccgaa tttaaagagc gtttgaaatg ggtctaaaaa tgtttgaaac
     7321 taattgaatg tgtgtataat agtaaaaatg cagtaaatat cccgacaatt tctattgaca
     7381 attcaaacag attggtttat aattaatata acaacaaatg aaagcgcaaa ctttcgcggt
     7441 cggaaggtag ttttatgaca cgatttgaga tacgagatga tttctatctc gatggaaaat
     7501 catttaagat tttatctggt gccattcatt attttaggat tcctccagag gattggtatc
     7561 attcgctcta taacttgaag gctcttggtt ttaatacggt agagacttat gttgcttgga
     7621 atttacacga gcctcgtgaa ggtgagtttc attttgaagg tgatctggat ttagagaaat
     7681 ttctccaaat agcgcaggat ttgggtctct acgcaattgt gcgtccgtct ccatttatct
     7741 gtgcggaatg ggaattcggt ggcttaccag cttggctctt gaccaagaac atgcgaattc
     7801 gctcatccga cccagcatat atcgaggcag ttggtcgcta ctatgatcag ttattgccaa
     7861 gactggtgcc tcgtttgttg aacaatggtg gcaatattct catgatgcag gttgaaaatg
     7921 agtatggttc ttacggagaa gataaggctt acctgagagc gattcgacag ctaatggaag
     7981 agtgtggcgt aacctgtccc ctctttacat cagatggtcc atggcgagct actctgaaag
     8041 ctggaacctt aattgaagag gacctctttg taacaggaaa ctttggttct aaggcacctt
     8101 acaacttttc gcagatgcag gaattctttg atgaacatgg taagaaatgg ccactcatgt
     8161 gtatggagtt ctgggatggt tggttcaatc gctggaaaga accgattatc acacgggatc
     8221 ctaaggaatt ggcagatgca gttcgagagg ttttggaaca aggctctatc aatctttaca
     8281 tgttccacgg tggtacaaac tttggtttca tgaatggttg ctcagctcga ggaactttgg
     8341 acctgccaca agttacatct tatgattacg atgcccttct ggatgaagaa ggaaatccaa
     8401 ctgctaaata tcttgcagtc aagaagatga tggcaacaca tttttcagag tatccgcagt
     8461 tggaaccact ctacaaagag agtatggagt tggatgctat tccactagtt gaaaaagttt
     8521 ctttgtttga aaccttagat agcttgtcaa gtcctgtaga aagtctctat cctcaaaaga
     8581 tggaggagct gggacaaagt tatggctacc tactttatcg aacagaaaca aactgggatg
     8641 cagaagaaga aagacttcgt atcattgatg gtcgagatag ggcccagctg tatgtcgatg
     8701 gtcagtgggt taaaactcaa tatcagacag agattgggga agatattttt tatcaaggta
     8761 aaaagaaagg gctatctagg ttagatatct tgatagaaaa tatggggcgt gtcaactatg
     8821 ggcataagtt cttagcggat acgcaacgta agggaattcg gacaggggtc tgtaaggatc
     8881 tgcatttctt actaaactgg aaacactatc cactcccact agacaatcct gagaaaattg
     8941 atttttcaaa aggatggact caaggacaac cagcctttta cgcttatgac tttacagtcg
     9001 aagagccaaa agatacttac ctagacttgt ctgagtttgg taagggagtt gcctttgtca
     9061 atgggcagaa tctaggacgt ttttggaacg ttggcccaac tctctcactt tatatccctc
     9121 atagctatct caaggaaggt gccaaccgta tcattatctt tgaaacagaa ggtcaatata
     9181 aagaagagat tcatttaact cgtaaaccta cactaaaaca tataaagggg gaaaacttat
     9241 gacaattgta ggatgccgta ttgatggacg tttgatccac ggacaagtag ccaatctttg
     9301 ggctggaaaa ctaaatgttt cacgcattat ggttgtagac gacgaagttg tcaacaacga
     9361 tattgaaaag agtggtttga aacttgcgac accaccaggt gtgaaattga gtattttgcc
     9421 agttgagaaa gctgcagcca atattcttgc tggtaaatac gatagccaac gtctctttat
     9481 cgtggctcgt aaaccagacc gcttccttgg tttggtagaa gcaggtgtac cacttgaaac
     9541 ccttaatgtt gggaatatgt ctcaaacacc agaaactcgt tctattacac gttctatcaa
     9601 cgtagtagac aaggatgtgg aagacttcca caaactggca gaaaaaggtg ttaaacttac
     9661 tgctcagatg gttccaaatg atccaatttc agactttttg agcttattaa aataggaaaa
     9721 aaatttttag gaggtcattg ttatgataca atggtggcaa attttacttc tcactttgta
     9781 ctcagcttat caaatctgtg atgagttgac gatcgtttca tctgcaggtt cccctgtatt
     9841 tgctggtttc attactggtt taatcatggg agatgtgact actggtttac ttatcggtgg
     9901 taacttgcaa ctgttcgttc ttggggttgg taccttcggt ggtgcttctc gtatcgacgc
     9961 aacttctggt gcggttcttg cgacagcctt ctctgtttca caaggaattg atgcaccgct
    10021 tgccattact acaatcgctg taccagtagc agctctcttg acttacttcg acgttcttgg
    10081 tcgtatgact actaccttct tcgctcaccg tgtggatgct gcaatcgaac gctttgacta
    10141 taaaggtatt gaacgcaact acttgcttgg tgcgattccg tgggctctat ctcgtgccct
    10201 tccagtcttc tttgcccttg cttttggtgg tgcctttgta caatcagtag tagacttcgt
    10261 tgaagcctac aaatgggttg cagatggctt gacacttgca ggacgtatgc ttccaggtct
    10321 tggatttgca atcttgcttc gttaccttcc agttaaacgt aaccttcact accttgctat
    10381 gggatttggt ttgacagcta tgttgactgt tctttactca tatgtaacag gtcttggtgg
    10441 cgctgttgct ggtatcgtag gtactcttcc tgctgaagtt gctgaaaaaa ttggtttcgt
    10501 gaacaacttc aaaggtttgt ctatgattgg tatttctatc gtaggtattt tccttgcagt
    10561 gcttcacttc aaaaatagcc aaaaagtagc tgtagcagca ccttctacac catcagaaag
    10621 tggggaaatc gaagatgacg aattctaatt acaaacttac aaaagaagat tttaatcaaa
    10681 tcaacaaacg tagcttgttt actttccaat taggttggaa ctacgaacgt atgcaagctt
    10741 ctggttacct ttacatgatc ttgcctcagt tgcgtaaaat gtatggtgat ggaactcctg
    10801 aattgaaaga aatgatgaaa gttcatactc aattcttcaa tacttcacca ttcttccata
    10861 ccattatcgc tggttttgac cttgccatgg aagaaaaaga tggtgtaggt tcaaaagacg
    10921 ctgttaacgg tatcaagaca ggtttgatgg gaccattcgc tcctcttggg gatacaatct
    10981 ttggttcact tgtacctgct atcatggggt cagtcgcagc aactatggct atcgctggcc
    11041 aaccttgggg gatcttcctt tggattgcag ttgcagtagc gtatgacatc ttccgttgga
    11101 aacagttgga atttgcttac aaagaagggg ttaaccttat caacaacatg caaagtacct
    11161 tgacagcttt gattgacgct gcatctgtac ttggtgtctt catgatgggt gctcttgtag
    11221 caacagtgat taactttgaa atttcttaca agttgccaat cggtgaaaag atgattgatt
    11281 tccaagacat cttgaaccaa atcttcccac gtttgcttcc agcaatcttt actgccttta
    11341 tcttctggtt gcttggtaag aaaggtatga actctactaa agctatcggt attattatcg
    11401 tacttgcttt gactctttct gcccttggtc actttgcact tggaatgtaa ttccttatga
    11461 ctaaatcatt aattttggtg agccatggtc gcttctgtga ggagcttaga ggtagcacag
    11521 aaatgattat gggcccacaa gacaacattt acacagtagc tcttcttcca gaagatggcc
    11581 cagaagaatt tactgctaaa tttgaagctg ttattggagg attggatgat ttcctagtct
    11641 ttgcggatct tctcggtggg acaccttgta atgtggtgag tcgcttgatc atggaaggtc
    11701 gtgatattga cctttacgca gggatgaatc ttccaatggt gattgaattt atcaatgcga
    11761 gccttacagg cgcagatgcg gactacaaga gccgtgctgc agaaagcatt gtgaaagtta
    11821 atgacctgtt agcgggcttc gatgatgacg aagatgaata atactcttcg aaaatctctt
    11881 caaactacgt caacgtcgcc ttgccgtagg tatatgttac tgacttcgtc agtcttatcc
    11941 ggcaacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatctgcaa cctcaaagta
    12001 gtgctttgag cagcctgcgg ctagtttcct acagatttta gttggaactc gattcaattc
    12061 atgtgacaac gtgaaaatcg ttagagcatt ttatatagaa tatacatggg aatggagctt
    12121 actcccattc ccatatttaa tagaaaaaga ggaactcaat gctacattat acaaaagaag
    12181 acttgctcga attgggtgca gaaatcacta cgcgtgaaat ctaccaacaa cctgatgtat
    12241 ggagagaagc ttttgaattt tatcaagcaa aacgtgaaga aattgcagcc ttcctacaag
    12301 aaatcgctga taaacatgac tatattaagg ttatcttgac aggtgctggg acttctgctt
    12361 atgtgggaga caccttgcta ccttacttta aggaagtcta tgacgaacgc aaatggaatt
    12421 tcaatgctat tgcgacaaca gatatcgttg ccaatccagc aacctatttg aaaaaagatg
    12481 tggcaactgt ccttgtgtct tttgctcgta gtgggaactc gcctgaaagt gtggcgactg
    12541 ttgatttggc caaatccttg gtggatgagc tttatcaagt gacgattact tgtgcagcag
    12601 atggtaaatt ggctcttcaa gctcacggtg atgaccgtaa tctcttgctc ttgcaaccag
    12661 ctgtctctaa tgatgctgga tttgccatga cttctagctt tacgtctatg atgttgacag
    12721 ctctcttggt ctttgatcct acagaatttg ctgttaagtc tgaacgtttt gaagttgtat
    12781 ctagtcttgc ccgtaaagtt ttagacaagg cagaagatgt caaaaagctc gttgatttag
    12841 actttaaccg tgtcatctat ctaggcgctg gtcctttctt tggacttgct catgaagctc
    12901 agctcaagat tttggaatta actgctggtc aagttgcgac catgtatgaa agcccagttg
    12961 gcttccgtca cggtccaaaa tctcttatca acgacaatac agttgttttg gtctttggta
    13021 caacgacaga ctacactcgt aagtacgact tggacttggt tcgtgaagtt gctggtgacc
    13081 agattgctcg tcgtgttgtg cttttgagtg atcaagcttt tggtcttgaa aatgtcaaag
    13141 aagtggccct tgattgtggc ggtgtcttga atgatattta ccgtgtcttc ccttacatcg
    13201 tttatgccca actctttgcc ctattgactt cactcaaggt agaaaataaa ccagatacac
    13261 cgtctcctac aggtacagta aaccgtgtag tacaaggtgt cataattcac gaatatcaaa
    13321 agtaagacag tgtttatgaa ttcttgacaa gaggatttgt aaattatcag ataaaccata
    13381 gattgtcagt acgctttcta tggtttgttt gcttgagaga aatagtaaaa ggagaacaga
    13441 atgaaagcat acacagagcg tgtatttgga aatgttgagg gcgaggatgt cttggcctat
    13501 cgatttgaga cagacggtgg ctaccaactt gaggttatga cttatggcgc gaccatcttg
    13561 cgctatgtcg cacctgacaa ggctggaaat tttgccaatg ttatcttggg atttgatgac
    13621 tttgatagtt atgtaggcaa tagtcccaag catggagcaa gtgtaggtcc tgtagcgggt
    13681 cgtattgcag gtgcgacctt tgagctcaat ggtaagacct atgaccttga ggttaataat
    13741 gctagcaact gtaatcacag tggttcaact ggttgggatt ccagcttgtt tgaagttgaa
    13801 gaagtaagcg atcatggctt gactctctac acagagcgta cagatgggac aggagggttc
    13861 cctggaaatc tcaagatttg gatcagttat cacttggaag aaactggtgc ctatgaaatc
    13921 agctacaagg taacgaccga tcaggatacg ctggtcaatc caaccaacca cagctatttc
    13981 aacttgtctg gtgatttcac gcagacgatt gaccgtcatg tcttccaact aaacacagag
    14041 ggcatttact caatcgctcc tgacggtgtt cctgccaaaa ctccagaagc caaccgtgat
    14101 gtggtcaaac acatctacaa tggtgccttg ttgaaggata tctttgcaga agaagatgag
    14161 caaatccagc tggcatcagg tttggatcat ccatttgccc ttcctgcagg ccatgacaat
    14221 gctggattcc tttatgacca aaattcaggt cgcttcctgc ttttcaagac agaagctcct
    14281 tgctttgtgg tctacacagc aaactttgtg gatgaaagtg tcatcatagg aggtcagcca
    14341 atgctacagc acaatgggat tgctcttgaa gcgcaagctt taccagatgc cattcacagt
    14401 gaccttaaag gccaagtcat tcttaaagct ggtcaaacct tcaccagtaa gacacgttat
    14461 gaacttgttg tgaaataaaa aagctggtca aaaagtcttt aaaattaaaa taacgcatat
    14521 tgtcaggtga tgaaaagttt gataatatgc gttttattat gaaatgattt actttatatt
    14581 atcctgaaat ggtgttattt ttgagttatc tggcttgact ttcttgatgg aaattatata
    14641 atagttgcaa caataataat tacaacgaaa aaagaggacc atttatgacc tatgaataca
    14701 agagtcacat ttatttggca gagacagttt taaatgtaaa ggatttggcc agtcaaacag
    14761 ccttttatca gcaagttatc ggtttagaaa tcttatctca aactgagaca gagtccattc
    14821 taggccttgg tggaaaagtc ttggtacagc tgattcaagc acaagagagc ggagaagtga
    14881 gggaacatta tggtctttac catttggcca ttctcttgcc cacacgcaag gctttggctg
    14941 atgtcttgaa acacctgaca gatttacaga ttcctcttgt cggcggtgca gaccatggtt
    15001 acagtgaagc tatttacttg gaagatttgg agggaaatgg cattgaactc tatcgagata
    15061 agccagtttc cacatgggat attcgagaag atggacgcat tatcggagtg acggaggctc
    15121 ttgcggcgca ggacatttat gagttggggg aaagagtaga gccctttatc ctggcagaag
    15181 gtacgagaat ggggcatatt catctttcag tcaaggatag tcgaaagtcc agccagtttt
    15241 atcaaaaggt gttagggcta gaggataaat tcagtgtgcc tagcgctagt tggattgcgg
    15301 ctggagatta ccatcatcat ttagcagtta acgaatgggg aggaaaaggt ctggcttcgc
    15361 gtaagcaagg cttaccaggt ttagcctact atgtcatcga agtcgcacat aaaaaagaac
    15421 tgttaacgat tgcccaacga gcacaagaag ttgacgcacc aatcaaatgg atgacatcga
    15481 gccaattgga aatcacagac tcagatggca tcgtgacccg tattcgttta gcaaggtaaa
    15541 attaggaatt tttaataagt tataagtatt gctagtttgc tttttgaacg gtctttggta
    15601 acagaactgc gtgagctgga tgggtgagaa ttttgacaaa gaagctgatt agagatataa
    15661 tgaagaaaag tcgagcaagg aaaaagaaat gacaaattca aagtatatta cttgtttgaa
    15721 acgttcagag ggccagttgt gtgggattca aaagatgatt gaaggagatc gtgactgtgc
    15781 tgatcttgtg acgcaactga cagcagtgag atctagtgta gagcgcgtga ttgagatgat
    15841 aattaccgaa aatcttactg aatgtatcaa tcagccacta gatgattctg aagctcaaaa
    15901 ggaacgctta gaaaaggcta tccgatactt gattaaacgg aaataaatga atgttttgag
    15961 acgatatgtg caaaggtcta ttaactatta gaatagttta atgtgagtca aaagaagttg
    16021 taaaaaatct ttaaaattaa aaaaacgcat agtatcaggt attgaaatgt actgccccca
    16081 aaagttagat tttttctgtc taacttttgg ggggcagtac ataaaaacct tgatagtatg
    16141 cgttttattg tggggagatt tatactcttc gaaaatctct tcaaaccacg tcagcttcac
    16201 cttgccgtag atatggttac tgacttcgtc agttctatcc acaaccttaa aacagtgttt
    16261 tgagcagcct gcggctaact tcctagtttg cactttaatt ttcattgagt attacttcat
    16321 cttctcatga aattgagttt ttgcccagct cctcttgatt attctacttg accgggctaa
    16381 gcattcatac caccttctac gttggtaacg gtgaggcctt gggagctgag aaattggcag
    16441 gtagatgcag agcggactcc tccttgacaa ataacatagt attcatggtc aggtttgagt
    16501 tctttgtagc cttgctctaa ggtacttaat ggaagatttt tggcacctgg tgcatgtcct
    16561 gcttggaatt catgtgcttc acggacatcg ataagttcta gattttcatt ttgatatttt
    16621 ccataaaagt cagccatgct gatactagtt tccatattct actccttctg ggttagccat
    16681 tttgtatagt gaataagcgc cgtcaaggtt ttggacggta aatcctgctt gcttgaggat
    16741 aggctctgcg atatagctgc gcaaactact gtggcaactg acgatgtagg cttgtttttc
    16801 tcctcagttc atcagcttcc tccttgacac tcggtcagat tttgatacaa tagtacaaaa
    16861 ttagaggagg tgggctatga ttcagaaaca tgcgattccc attttagagt ttgatgataa
    16921 tcctcaggcg gttatcatgc ccaatcacga ggggttggat ttgcagttgc caaagaagtg
    16981 tgtctatgct tttttaggtg aggagattga ccgctatgcg agggaagtag gggcgaactg
    17041 tgttggcgaa ttcgtttctg ctaccaagac ctatccagtc tatgtcatca actacaaggg
    17101 tgaggaggtc tgtctggctc aggctcctgt tggctccgct ccagcagccc agtttatgga
    17161 ttggttgatt ggctatggtg tggagcagat tatctctact gggacctgtg gtgtcctagc
    17221 tgatatagag gaaaatgcct ttctagtccc tgttcgcgct ttgcgagatg agggagccag
    17281 ttaccactat gtggcacctt gtcgttatat ggaaatgcag ccagaggcta ttgctgctat
    17341 tgaggaagtt ttggaagaca gagggattcc ttatgaagaa gtcatgacct ggacgacaga
    17401 cggtttttac cgagaaacgg ctgaaaaggt ggcttatcgt aaggaagaag gctgtgctgt
    17461 tgtggagatg gagtgttctg ctcttgctgc agtagctcaa ttgcgtgggg ttctctgggg
    17521 tgaattgttg ttcacagcag attctctagc ggacttggac cagtacgaca gtcgtgactg
    17581 gggctcggaa gcttttaata aggcgctaga actgagttta gcaagtgttc accaccttta
    17641 gttgtactgg caaaggattt gttttatcat aaaatgtcta gctcatactt ttcaaaaata
    17701 tgtttaaacg aggtcacctt cctcttgtcc taggcatgtt gaggttggga aaaatcttta
    17761 aaatcagaaa aacgtatcat atcaggtgat gaaaattttg atactatgcg ttttatgttg
    17821 gtaagattta gagtgagatg aaatgatact cttcgaaaat ctcttcaaac caggtcagct
    17881 tcaccttgcc gtatagatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg
    17941 ttttgagctg acttcgtcag ttttatctgc aacctcaaaa cagtgttttg agctgacttc
    18001 gtcagttcta tccacaacct caaaacagtg ttttgagcaa cctgtgacta gctttctaat
    18061 cgatgccttg gttttcattg cctataatca aaaagagaaa ttttctcctg aaaagcatat
    18121 agagtagctg gcgttaaaag ctcctgtctt gcttttttga cctatagtca tatctatcaa
    18181 gtattgttct tgcctaagct atcaataaaa aggtggcatt ttttaggctt ggtgttagtg
    18241 aattttgcct tatcctatct aagtcatttc gagcttttta tggtacaatg gaaacatgtt
    18301 attcaaatta tctaaggaaa aaatagagct aggcttatct cgtttatcgc cagcccgtcg
    18361 tatttttttg agttttgcct tggtcatttt actaggctct cttcttttga gcttgccctt
    18421 tgtccaagtt gaaagctcac gagcgactta ttttgatcat cttttcactg ctgtctctgc
    18481 agtctgtgtg acaggtctct caacccttcc agtagctcac acctataata tctggggcca
    18541 aataatctgt ttgctcttga ttcagatcgg tggtctaggg ctcatgacct ttattggggt
    18601 tttctatatc cagagcaagc aaaagcttag tcttcgtagc cgtgcaacta ttcaggatag
    18661 ttttagttat ggagaaactc gatctttgag aaagtttgtc tattctattt ttctcacgac
    18721 ctttttggtt gagagcttgg gagctatttt gcttagtttt cgccttattc ctcaacttgg
    18781 ctggggacgt ggtcttttta gttccatttt tctagcgatc tcagccttct gtaatgccgg
    18841 ttttgataat ttagggagca ccagtttatt tgcttttcag accgatttac tggtcaatct
    18901 ggtgattgca ggcttgatta ttacaggcgg ccttggtttt atggtctggt ttgatttggc
    18961 tggtcatgta ggaagaaaga aaaaaggacg tctgcacttt catacgaagc ttgtattatt
    19021 attgactata ggtttgttgt tatttggaac ggcaactact ctctttcttg agtggaacaa
    19081 tgctggaacg attggcaatc tccctgttgc cgataaggtt ttagttagct tttttcaaac
    19141 agtgacgatg cgaacagctg gcttttctac gatagattat actcaggctc atcctgtgac
    19201 tcttttgatt tatatcttac agatgtttct aggtggggca cctggaggaa cagctggggg
    19261 actcaagatt acgacatttt ttgtcctctt ggtctttgca cgaagtgagc ttctaggctt
    19321 gcctcatgcc aatgttgcga gacgaacgat cgcgccgcga acggttcaaa aatcctttag
    19381 tgtctttatt atctttttga tgagcttctt gataggattg attctgctag ggataacagc
    19441 caaaggcaat cctcccttta tccacctcat atttgaaacc atttcagctc ttagtacagt
    19501 tggtgtaacg gcaaatctga ctcctgacct tgggaaattg gctctcagtg ttatcatgcc
    19561 acttatgttt atgggacgaa ttggtccctt gaccttgttt gttagcttgg cagattacca
    19621 tccagaaaag aaagatatga ttcactatat gaaagcagat attagtattg gttaagaaag
    19681 gaaagagcat gtcagatcgt acgattggaa ttttgggctt gggaattttt gggagcagtg
    19741 tcctagctgc cctagccaag caggatatga atattatcgc tattgatgac cacgcagagc
    19801 gcatcaatca gtttgagcca gttttggcgc gtggagtgat tggtgacatc acagatgaag
    19861 aattattgag atcagcaggg attgatacct gcgataccgt tgtagtcgcg acaggtgaaa
    19921 atctggagtc gagtgtgctt gcggttatgc actgtaagag tttgggggta ccgactgtta
    19981 ttgctaaggt caaaagtcag accgctaaga aagtgctaga aaagattgga gctgactcgg
    20041 ttatctcgcc agagtatgaa atggggcagt ctctagcaca gaccattctt ttccataata
    20101 gtgttgatgt ctttcagttg gataaaaatg tgtctatcgt ggagatgaaa attcctcagt
    20161 cttgggcagg tcaaagtctg agtaaattag acctccgtgg caaatacaat ctgaatattt
    20221 tgggtttccg agagcaggaa aattccccat tggatgttga atttggacca gatgacctct
    20281 tgaaagcaga tacctatatt ttggcagtca tcaacaacca gtatttggat accctagtag
    20341 cattgaattc gtaaagaggg atgacccctc ttttttgatg cttaagatgg caaatagaga
    20401 cagaagcccc ttgtcttcta gtaaaagttc ttcaaaggct ggactttatg gtaaaataga
    20461 aggaagtgac aagagagagt aatactcaat gaaaatcaaa gatcaaacta ggaaactagc
    20521 tgcgggctgc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagctcaaa
    20581 acactgtttt gaggttgcag atagaactga cgaagtcagc tcaaaacact gttttgaggt
    20641 tgcagataga actgacgaag tcagtaacat ctatacggca aggcgacgtt gacgcggttt
    20701 gaagagattt tcgaagagta taagaaaaaa tcagtcccct aaaggagcag attatgaagt
    20761 tattgtctat cgccatttct agctataatg cagcagccta tcttcattac tgtgtggagt
    20821 cgctagtgat tggtggtgag caagttggga ttttgattat caatgacggg tctcaggatc
    20881 agactcagga aatcgctgag tgtttagcta gcaagtatcc taatatcgtt agagccatct
    20941 atcaggaaaa taaaggccat ggcggtgcgg tcaatcgtgg cttggcagag gcttctgggc
    21001 gctattttaa agtagttgac agtgatgact gggtggatcc tcgtgcctac ttgaaaattc
    21061 ttgaaacctt gcaggaattt gagagcaagg gtcaagaggt ggatgtcttt gtgaccaatt
    21121 ttgtctatga aaaggaaggt cagtcttgta agaagagtat gagttacgat tcagtcttgc
    21181 ctgttcggca gatttttggc tgggaccagg tcggaaattt ctccaaaggc cagtatatca
    21241 tgatgcactc gctgatttat cggacagatt tgttgcgtgc tagccagttc taattgcctg
    21301 aacatacttt ttatgtcgat aatctctttg tctttacgcc ccttcagcag gtcaagacca
    21361 tgtactatct gcctgtcgat ttctatcgtt atttgattgg gcgtgaggac cagtctgtca
    21421 atgagcaagt gatgattaag tgcattgacc agcaactcaa ggtcaatcga ctcttggtag
    21481 accaacttta tttgtcccaa gtgagtcatc ccaaaatgcg agaatatctg ctgaatcata
    21541 ttgaaatcac gacggtgatt tccagtaccc tgctcaaccg atctggaaca gcggagcatc
    21601 tggcaaaaaa acgccaattg tggacctata ttcagcagaa aaatccagaa gtctttcagg
    21661 ctattcgtaa gaccatgttg agccgtttga ccaaacattc tgtcttgcca gatcgcaaac
    21721 tgtccaatgt cgtctatcaa atcaccaaat ctgtttatgg atttaattaa tataagtgtt
    21781 ttataagagg gatttaagaa aaattttaac tttttcttag tcctttttaa tttcaggaga
    21841 ttatactaga gtcatcaaat aaagaaagac tctaaggaga atcctatgaa attcaatcca
    21901 aatcaaagat atactcgttg gtctattcgc cgtctcagtg tcggtgttgc ctcagttgtt
    21961 gtggctagtg gcttctttgt cctagttggt cagccaagtt ctgtacgtgc cgatgtggtc
    22021 aatccgaccc caggtcaagt cttacctgaa gagacatcgg gaacgaaaga gggtgactta
    22081 tcagaaaaac caggagacac cgttctcact caagcgaaac ctgagggcgt tactggaaat
    22141 acgaattcac ttccgacacc tacagaaaga actgaagtga gcgaggaaac aaactcttct
    22201 agtctggata cactttttga aaaagatgaa gaagctcaag aaaatccaga gctaacagat
    22261 gccttaaaag aaactgtaga tacagctgat gtggatggga cacaagcaag tccagcagaa
    22321 actactcctg aacaagtaaa aggtggagtg aaagaaaata caaaagacag catcgatgtt
    22381 cctgctgctt atcttgaaaa agctgaaggg aaaggtcctt tcactgctgg tgtaaaccaa
    22441 gtaattcctt atgaactatt cgctggtgat ggtatgttaa ctcgtctatt actaaaagct
    22501 tcggataatg ctccttggtc tgacaatggt actgctaaaa atcctgcttt acctcctctt
    22561 gaaggattaa caaaagggaa atacttctat gaagtagact taaatggcaa tactgttggt
    22621 aaacaaggtc aagctttaat tgatcaactt cgcgctaatg gtactcaaac ttataaagct
    22681 actgttaaag tttacggaaa taaagacggt aaagctgact tgactaatct agttgctact
    22741 aaaaatgtag acatcaacat caatggatta gttgctaaag aaactgtcga aaaagctgtt
    22801 aaagataacg ttaaagacag tatcgatgtt ccagcagcct acctagaaaa
//
LOCUS       DAVLFB010000027        22035 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLFB010000027 DAVLFB010000000
VERSION     DAVLFB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.027"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            91..2313
                     /gene="recJ"
                     /locus_tag="VXD58_000924"
     CDS             91..2313
                     /gene="recJ"
                     /locus_tag="VXD58_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV0675654.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2488..4149
                     /locus_tag="VXD58_000925"
     CDS             2488..4149
                     /locus_tag="VXD58_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0675655.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4218..4997
                     /locus_tag="VXD58_000926"
     CDS             4218..4997
                     /locus_tag="VXD58_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0675656.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5109..6329
                     /gene="murM"
                     /locus_tag="VXD58_000927"
     CDS             5109..6329
                     /gene="murM"
                     /locus_tag="VXD58_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV0675657.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6334..7566
                     /gene="murN"
                     /locus_tag="VXD58_000928"
     CDS             6334..7566
                     /gene="murN"
                     /locus_tag="VXD58_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV0675658.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRVIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7645..8346
                     /locus_tag="VXD58_000929"
     CDS             7645..8346
                     /locus_tag="VXD58_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV0675659.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8386..10233
                     /gene="uvrC"
                     /locus_tag="VXD58_000930"
     CDS             8386..10233
                     /gene="uvrC"
                     /locus_tag="VXD58_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV0675660.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10223..11056
                     /locus_tag="VXD58_000931"
     CDS             10223..11056
                     /locus_tag="VXD58_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0675661.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGTIGMPYFNWEVLKNHRSQYAVIEVEDGELVNILFRKV
                     AYDYEAELKLAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11238..12038)
                     /locus_tag="VXD58_000932"
     CDS             complement(11238..12038)
                     /locus_tag="VXD58_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675662.1"
                     /translation="MKKFSLLLAILPLLVACGNQATPKETNSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAERYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12209..12814
                     /locus_tag="VXD58_000933"
     CDS             12209..12814
                     /locus_tag="VXD58_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV0675663.1"
                     /translation="MKFLELNKKRHATKKFTDKSVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIADRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12827..14227
                     /gene="pepV"
                     /locus_tag="VXD58_000934"
     CDS             12827..14227
                     /gene="pepV"
                     /locus_tag="VXD58_000934"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV0675664.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14281..14859
                     /locus_tag="VXD58_000935"
     CDS             14281..14859
                     /locus_tag="VXD58_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV0675665.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15019..15171
                     /locus_tag="VXD58_000936"
     CDS             15019..15171
                     /locus_tag="VXD58_000936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0675666.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            15214..15306
                     /locus_tag="VXD58_000937"
     CDS             15214..15306
                     /locus_tag="VXD58_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0675667.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLVFEEL"
     gene            15477..16802
                     /gene="brnQ"
                     /locus_tag="VXD58_000938"
     CDS             15477..16802
                     /gene="brnQ"
                     /locus_tag="VXD58_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV0675668.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16900..17937
                     /locus_tag="VXD58_000939"
     CDS             16900..17937
                     /locus_tag="VXD58_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV0675669.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYIKSALVD"
     gene            17939..18409
                     /locus_tag="VXD58_000940"
     CDS             17939..18409
                     /locus_tag="VXD58_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0675670.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLEKETRLRFLEEMSLVQEAVAKAFTAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLNGRGPVWWVPFEEMTAETCQAKSDEIKQLVKRLSSELDKLLEIKE"
     gene            18412..19128
                     /locus_tag="VXD58_000941"
     CDS             18412..19128
                     /locus_tag="VXD58_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV0675671.1"
                     /translation="MKKRYLVLTALLTLSLAACSQEKAKNEDGEAKTEQTAKTDGTVG
                     SKSQGTAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            19392..19817
                     /gene="rplK"
                     /locus_tag="VXD58_000942"
     CDS             19392..19817
                     /gene="rplK"
                     /locus_tag="VXD58_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV0675672.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20025..20714
                     /gene="rplA"
                     /locus_tag="VXD58_000943"
     CDS             20025..20714
                     /gene="rplA"
                     /locus_tag="VXD58_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV0675673.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            20987..21145
                     /locus_tag="VXD58_000944"
                     /pseudo
     CDS             20987..21145
                     /locus_tag="VXD58_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(21372..21452)
                     /locus_tag="VXD58_000945"
                     /pseudo
     CDS             complement(21372..21452)
                     /locus_tag="VXD58_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21460..21822
                     /locus_tag="VXD58_000946"
     CDS             21460..21822
                     /locus_tag="VXD58_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV0675674.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6608 a         4056 c         5090 g         6281 t
ORIGIN      
        1 aaatgttata aaaaagcaat ctggatagag aaaacgtcta aatcatgtta taatgaagca
       61 atagaattct tagaaggagt ggatgtcttt ttgataacac ctacttatga atggcagttt
      121 gccctgcagg tagaagatgc ggattttaca aagatagcca agaaggctgg actgggtcct
      181 gaggtggctc ggttattgtt tgagagaggg attcaggacc aagaaagtct gaagaagttt
      241 ttagaacctt ccttggagga cttacatgat gcttatctgc tccatgatat ggacaaggca
      301 gtggagcgga ttcgtcaggc tattgaagaa agggaaaata ttctcgttta tggagactac
      361 gatgcggatg gcatgacttc ggcttctatt gtgaaggaaa gtttggaaca acttggtgcg
      421 gagtgccgag tttacctgcc aaatcgtttt accgatggct atggccctaa tgctagtgtt
      481 tataaatact ttatcgagca agaaggaatt tccttgattg tgacggtgga caatggggtt
      541 gctggtcatg aggctattgc attggctcag tctatgggag tagatgtcat tgtgacagac
      601 catcattcca tgcctgaaac cctgccagat gcttatgcta ttgtccatcc tgaacatcca
      661 gatgcggatt atccttttaa atatttggct ggttgtggag ttgctttcaa gttggcttgt
      721 gccctgttag aagaagtgca agtggaattg cttgatttgg tcgctattgg aactattgca
      781 gatatggtga gtctgacgga tgaaaatcgt atcttagttc aatatggtct ggaaatgttg
      841 ggtcataccc agcgcattgg tctgcaagaa atgctggaca tggctgggat tgctgccaac
      901 gaagtaacag aagaaacggt tggtttccag attgctcctc gtttgaatgc cttgggtcgc
      961 ttggatgatc ccaatcctgc cattgatttg ttgactggat ttgatgatga ggaagcgcat
     1021 gagattgccc ttatgattca ccagaaaaac gaagagcgca aggaaatcgt tcagtctatc
     1081 tatgaagaag ccaagaccat ggtggatcct gagaagaagg ttcaggtctt ggccaaggaa
     1141 ggctggaatc ctggggttct aggaatcgtg gctggtcgtt tattggaaga attgggacag
     1201 acagtcattg ttcttaatat agaagacggt cgtgccaagg gcagtgctcg tagtgtggaa
     1261 gcggtcgata tttttgaagc tctggatccc catcgagacc tcttcatcgc ctttggaggt
     1321 catgcaggtg cagcgggtat gacgctggaa gttgagcaac tctcagattt atctcaggtt
     1381 ttggaagatt atgttcgtga aaaaggtgca gatgctggtg gcaagaataa gttaaaccta
     1441 gatgaagagt tggatttgga ggcacttagc ttggaaacgg tcaaaagttt tgaacgttta
     1501 gctccttttg gaatggataa tcagaaacct attttttata tcaagaattt tcaggtcgaa
     1561 agtgctcgta ctatgggggc aggtaatgcc catctaaagc tgaaaatttc caagggtggg
     1621 gcgagttttg aagtggtagc ctttggtcaa ggcagatggg cgacagagtt ttctcaaacc
     1681 aagaatctag agttagcggt taaattgtct gtcaaccaat ggaatggcca aactgccctc
     1741 cagttgatga tggtggatgc gcgagtggaa ggtgttcaac tttttaacat tcgtggaaaa
     1801 aatgcagtct tgccagaagg tgttccagtc ttggattttc ctggagaact gccaaatctt
     1861 gcggctagtg aagctattgt cgtaaaaaac attccagagg atattactca gctgaagacc
     1921 atttttcagg aacagcattt ctctgctgtc tatttcaaaa atgatattga caaggcctat
     1981 tatctgacag gttatgggac tagagatcag tttgccaaat tgtacaagac tatttaccag
     2041 ttcccagagt ttgatattcg ctacaagctg aaagatttgg ctgcatatct taatattcaa
     2101 caaatcttgc tggtcaagat gattcaagta tttgaagaac taggctttgt gacgataaaa
     2161 gatggtgtga tgacagtcaa taaagaggcg ccaaagcggg agataggaga aagtcaaatt
     2221 taccaaaatc tcaaacaaac cgttaaagac caagaaatga tggcgctggg tacggtgcaa
     2281 gaaatttatg attttttgat ggaaaaagag tagaagttag gaaagagttg ggaaatcaac
     2341 tcttttttga aaacagacct tcattttgaa aatcatcaaa aaaatggtat aatggtagga
     2401 aaagattcgg ctgaaagtat tagaactttt agaataagag ggtagagttg ccctataatc
     2461 aagataaact aagattttgg aggaaaaatg agtaatatca gtttaacaac acttggtggt
     2521 gtgcgtgaga atggaaaaaa tatgtacatt gctgaaattg gagagtccat ttttgttttg
     2581 aatgtagggt taaaatatcc tgaaaatgaa caattagggg tcgatgtggt gattccaaac
     2641 atggattacc tttttgaaaa tagcgaccgt attgctgggg ttttcttgac ccacgggcat
     2701 gcggatgcca ttggtgctct accttatctc ttggcagagg ctaaagttcc tgtatttggg
     2761 tctgagttga ccattgagtt ggcaaagctc tttgtcaaag gaaatgatgc cgttaagaaa
     2821 tttaatgatt tccatgtcat tgatgagaat acggagattg attttggtgg gacagtggtt
     2881 tccttcttcc ctacgactta ctccgttcca gagagtctgg gaattgtctt gaagacatcg
     2941 gaaggaagca tcgtttatac aggtgacttc aaatttgacc aaacggctag tgaatcttat
     3001 gcaactgatt ttgctcgttt ggcagagatt ggtcgtgacg gcgtcctggc tctcctcagt
     3061 gattcggcca atgcagacag caatattcag gtggctagtg aaagtgaagt tagggatgaa
     3121 attacccaaa ctattgctga ctgggaaggt cgtatcatcg ttgcagctgt ttccagtaat
     3181 ctttctcgta ttcagcagat ttttgacgct gcggataaaa caggtcgacg tatcgtcttg
     3241 acaggatttg atattgaaaa tatcgtccgc acagcgattc gtcttaagaa gttgtcttta
     3301 gccaacgaaa ttctcttgat taagcctaaa gatatgtctc gctttgaaga ccatgagttg
     3361 attattcttg agacaggtcg tatgggtgaa cctatcaatg gacttcgtaa gatgtcgatt
     3421 ggtcgccatc gttatgtaga aatcaaggat ggggacctgg tctatattgc tacggctccg
     3481 tctattgcta aagaagcctt tgttgcgcgt gtggaaaata tgatttatca ggcaggtggg
     3541 gttgtcaaat tgattaccca aagtttacat gtatcagggc acggaaatgt gcgtgatttg
     3601 cagctgatga tcaatctttt gcaacctaag tacctcttcc ctgtccaagg ggagtatcgt
     3661 gagttggatg ctcacgctaa ggctgccatg gcagttggga tgttgccaga acacatcttc
     3721 attcctaaaa aggggacgac catggcttac gagaatggag actttgttcc agctggatcg
     3781 gtttcagcag gagatatctt gattgatggg aatgccattg gtgatgttgg aaatgttgtt
     3841 cttcgtgacc gtaaggtctt gtcagaggat ggaattttca tcgtggctat tacagtcaac
     3901 cgtcgtgaga agaaaattgt ggctagggct cgtgttcata cgcgcggatt tgtttatctc
     3961 aagaagagtc gcgatattct ccgtgaaagt tcagaattga ttaaccaaac ggtagaagag
     4021 tatcttcaag gagatgactt tgactgggca gatcttaaag ggaaggttcg agataatttg
     4081 accaagtatc tctttgacca aaccaagcgt cgtccagcta ttttaccagt agtcatggaa
     4141 gcaaaataat cgttgaaata aacagagaga aagtcgagtt tcggcttttt cttatagaaa
     4201 aatagaagga gaaaatcatg gcagtaatga aaatcgagta ttactcacaa gtattggata
     4261 tggagtgggg ggtgaatgtc ctctaccctg atgccaatcg agtggaagaa ccagagtgtg
     4321 aagatattcc cgtcttgtac cttttgcacg ggatgtctgg aaatcataat agttggctta
     4381 agcggaccaa tgtagaacgc ttgcttcgag gaactaatct catcgttgtt atgcccaata
     4441 ccagcaatgg ttggtacacc gatacccagt atggttttga ctactacacg gctctagcag
     4501 aggaattgcc acaggttctg aaacgcttct tccctaatat gactagcaag cgtgaaaaga
     4561 cctttatcgc tggtctttct atgggaggct acggctgctt caaactggct cttgcgacaa
     4621 atcgtttttc tcatgcagct agcttttcag gtgccctcag ctttcaaaac ttttctcctg
     4681 aaagtcaaaa tctgggaagt ccagcctact ggagaggtgt ttttggagag attaaagact
     4741 ggacaactag tccctattct cttgaaagtc tggctaaaaa atcggataaa aagaccaaac
     4801 tttgggcgtg gtgtggcgaa caggatttct tgtacgaagc caataatctc gcagtgaaaa
     4861 atctcaaaaa actaggtttt gatgtgacct atagccatag cgctggaact cacgagtggt
     4921 actactggga aaaacaattg gaagtttttt taacaaccct accaattgat ttcaaattag
     4981 aagagagact gacttagttt gaacttcagc atagggggag tagaactaaa ataaaatatg
     5041 ttttcactag acttttcaaa cgaaagtagt agaatagtaa taaaatactg gaggaaagag
     5101 agtaggaaat gtaccgttat caaattggca ttcccacatt agaatatgat cagtttgtca
     5161 aagaacatga attagccaat gtattacaaa gtagtgcttg ggaggaagtt aagtctaatt
     5221 ggcaacatga gaagtttggt gtttacaggg aagaaaaatt actggcgaca gctagtattt
     5281 tgattagaac tcttccgcta ggctataaaa tgttttacat cccaagagga cctatattgg
     5341 attatgggga taaagaactc ttgaattttg tcattcagtc tattaagtcc tatgctcgca
     5401 gtaagagagc ggtttttgtg acttttgacc caagtatttg cctatctcaa agtttaatca
     5461 atcaggaaaa gacagaattt cctgaaaatc tggctattat tgatagtttg caacaaatgg
     5521 gagtaaggtg gtcaggaaaa acggaggaaa tgggagacac cattcaacct cgtattcagg
     5581 cgaaaatata caaggaaaat tttgaagaag ataaactttc caagtcaaca aaacaggcta
     5641 ttcgaacagc acgaaacaaa gggcttgaga ttcaatatgg tggactggaa ctattagatt
     5701 cattttcgga gttgatgaaa aaaactgaga agcgaaaaga gattcatttg aggaatgaag
     5761 cctattataa aaaattgtta gataatttta aggacaaggc ctatatcacc ttggccacct
     5821 tggatgtttc taaacgttcg caagagttag aagaacagtt agcgaaaaat agagccttgg
     5881 aagagacctt tactgagtcg actcgaactt caaaagtaga agcgcagaag aaggaaaaag
     5941 aacgtttgtt agaggaattg accttcttgc aggaatatat agatgtaggt caagcgagag
     6001 ttcctttagc ggctactttg agtttggaat ttggtactac ctctgtcaat atatatgctg
     6061 gtatggatga tgattttaaa cgttacaatg caccaatttt aacttggtat gaaacggctc
     6121 gctatgcctt tgaacgaggt atgatctggc aaaatttagg tggtgttgaa aactctctca
     6181 atggtggact ttatcatttt aaggaaaaat ttaatccaac gattgaagaa tacttgggtg
     6241 aatttacaat gcccactcat cctctctatc ctctgttaag acttgctctt gatttccgta
     6301 aaacattaag aaaaaaacat agaaagtaag tatatggcac taacaacact cacgaaagaa
     6361 gagtttcaga cttattctga tcaggtttct tctcgttcct ttatgcaatc tgtccagatg
     6421 ggggatttgc tagaaaaaag aggggctcga attgtttatc ttgctttgaa acaagaagga
     6481 gaaattcaag ttgcagctct ggtttatagc ctgcccatgc tgggtggtct gcatatggaa
     6541 ctcaattcgg ggccgattta tacccaacaa gatgctcttc cagtttttta tgcagagtta
     6601 aaagaatatg ccaagcaaaa tggtgtatta gagttgcttg taaaacccta tgaaacttat
     6661 caaacttttg atagccaagg taatccaata gatgctgaga aaaaaagtat tattcaagat
     6721 ttgactgatt taggttatca atttgatggc ttaacaacag gttacccagg tggagaacca
     6781 gattggttat actataaaga tttaactgaa ttaactgaaa agagtttgct taaaagtttt
     6841 agcaaaaagg gtaaaccctt ggtgaaaaag gctgaaacct ttggcattcg gttgaaaaag
     6901 ttaaaacgtg aagaactatc gatttttaag aatataacaa aagaaacctc tgaacgtaga
     6961 gaatatagtg ataaaagttt agaatattat gagcattttt atgatacttt tggagaacaa
     7021 gcggaatttc tcatagcaag cttaaatttt tcggactata tgagcaaatt gcaaggtgaa
     7081 caaagtaaac tagaagaaaa cttggacaag ttgcgacttg atttgagtaa aaatcctcat
     7141 tctgagaaaa aacaaaatca actgagagaa tattctagtc aatttgaaac gtttgaagtt
     7201 cgaaaagcag aagcgcgaga cttgattgaa aaatatggag aagaagatat tgttttagct
     7261 gggagtttat ttgtttatat gcctcaggaa acgacttatc tctttagtgg ttcctacact
     7321 gagtttaata agttctatgc ccctgccctg cttcaaaaat atgttatgtt ggaaagcata
     7381 aaacgtgtaa tacctaaata caacttccta ggcattcaag ggatttttga tggaagtgat
     7441 ggtgttttgc gttttaaaca gaattttaat ggctatattg tacgcaaagc gggtactttc
     7501 cgttaccatc catcgccttt aaaatacaaa gctatccagt tactcaaaaa aatagtagga
     7561 cgttaagatg aaaaagtcag tatttagatt tcttttagct tcttttagta aaataattct
     7621 tatttgctag aaaggtggag agacatgcgc tggctttttc gtttgatagg ggctttcttt
     7681 tcttttgtgt ggcgtttgtt ttggcgtctg gtttggatag ttgtgctctt atgtgtgctt
     7741 gctttcggac ttctctggta tctgaacggg gattttcaag gagcgctaaa gcaagcagaa
     7801 cggtcagtaa aaattggtca acaaagtatt gaccaatggg agaaaacagg gcaactgcct
     7861 aagttaagcc agacagatag tcaccagcat tctgaaggaa ggtgggcaca ggcctctgct
     7921 cgtatttacc tggatccgca gatggattca cgctttcaag aggcttattt agaagcaatc
     7981 cagaactgga atcaaactgg tgcttttaac tttgaactcg tgactgagtc tagtaaggcg
     8041 gatattacgg ctacggagat gaacgacgga ggcactcctg tggcaggaga ggcggaaagt
     8101 caaactaatc tcttaacagg gcaattcttg tccgtaacgg tgcggttgaa tcattattat
     8161 ttgtccaatc catactatgg ctactcctat gaacgccttg tccatacggc agaacatgag
     8221 ttaggtcatg cgattggctt ggaccataca gatgagaagt ctgtcatgca accagcaggt
     8281 tccttttatg gtatccagga agaggatgtt gcaaacctcc gaaaaatata tgagactagt
     8341 gagtagggta ctatctttcc ctactttttt tgctataatg gaactatgaa caacttgatt
     8401 aaatcaaaac tagagctctt gccgaccagc cctggttgct acattcataa ggataaaaat
     8461 ggcaccatta tctatgtagg aaaggctaaa aatctgcgta atcgagtacg gtcctatttt
     8521 cgtggaagtc atgataccaa gacagaggct ctggtgtctg aaattgtgga ttttgaattt
     8581 attgttacgg agtctaatat tgaggcactt ctcctagaaa tcaacctgat caaggaaaac
     8641 aagcccaagt acaatatcat gctcaaggat gacaagtcct atcctttcat caaaatcacc
     8701 aatgagcgct atccacgctt gattatcact cgtcaggtca aaaaggacgg aggtctttat
     8761 tttggaccct atcccgatgt gggggcagcc aatgaaatca agcggttgct ggatcggata
     8821 ttcccttttc gtaagtgtac caacccgccc tctaaggtct gtttttatta ccatatcggc
     8881 cagtgtatgg ctcacaccat ctgtaagaag gatgaggctt atttcaagtc tatggcccag
     8941 gaggtttctg attttctgaa agggcaggat aacaaaatca ttgatgagct aaagggcaaa
     9001 atggcagcgg cagcccagac tatggagttt gaacgtgcgg cggaataccg tgacctgatt
     9061 caggctattg gaacgcttcg aaccaagcaa cgggtcatgg ctaaggatct ccaaaatcgc
     9121 gatgtctttg gctactatgt ggataagggc tggatgtgtg tgcaggtttt ctttgttcgt
     9181 cagggcaagc tcatcgagcg cgatgtcaat ctcttcccct acttcaatga tccagatgag
     9241 gatttcttga cctatgtggg acaattctat caagaaaaat ctcacctagt tcccaatgag
     9301 gtactgattc cgcaggatat tgacgaagaa gctgtcaagg ctttggtgga taccaagatt
     9361 ctcaaacccc aacgtggaga gaaaaaacag ctggtcaatc tagccataaa aaatgcacgt
     9421 gttagtctgg agcagaaatt taacctgcta gaaaaatcag tcgaaaagac ccaaggagct
     9481 attgaaaatc taggacgttt gctccaaatt ccgactccag ttcgcatcga gtccttcgat
     9541 aactctaaca tcatggggac cagtcctgtt tcagccatgg tggtctttgt taacggcagg
     9601 ccaagtaaaa aagattaccg taagtacaag ataaaaaccg ttgttgggcc ggacgactat
     9661 gctagtatga gagaagtcat tcgaagacgc tatggtcgag tacagcgtga ggctttgact
     9721 cctccagatt tgattgtgat tgatgggggg caaggtcaag tcaatattgc taagcaagtc
     9781 atccaagaag aactgggctt ggatattcca attgcaggtc tgcaaaagaa tgataagcac
     9841 caaacccatg aattgctctt tggagatccg ctggaagtgg tggacttgtc tcgcaattct
     9901 caggaatttt tcctcctcca acgcattcaa gatgaggtcc accgctttgc cattaccttc
     9961 caccgccaac tgcgctccaa aaattctttc tcatctcaat tggatgggat tgatggtctg
    10021 ggacctaaac gcaagcaaaa tctcatgagg cacttcaagt ctctgaccaa aatcaaggaa
    10081 gccagtgtgg atgagattgt cgaagttgga gtaccaagag cagtagcaga ggctgtgcaa
    10141 agaaagttga acccgcagga aacagaaatc ttgcttcaag tggcggaaga aagagtagat
    10201 taccaaacgg aaggaaacca caataaacca taaaatcgca attttatcag atattcatgg
    10261 caatgcgacg gcgctagaag cagtgattgc agatgctaaa actcaaggag ccagtgaata
    10321 ttggctcatg ggagacattt tcctccctgg ttcaggtgca aatgacttgg tcgctctgtt
    10381 aaaggaactt cctatcacag caagtgttcg aggcaattgg gatgatcgtg tcctggaggt
    10441 cttggatggc gaatatggtt tggaatatcc gaaagaaatc cagtcaatgc gcatgaccca
    10501 gtttttgatg gagcgaatgg atcctgcaac gattgtctgg ctacgaagct tgcctttgct
    10561 ggaaaagaaa gaaattgacg gattgcgctt ttctatctct cataatttgc caaataagaa
    10621 ctatggtggt gacttgctag ttgagaatga tacagagaaa tttgaccaac tgctagatgc
    10681 ggaaacggac gtggcagttt atggtcatgt tcacaagcag ttgcttcgtt atggaagtca
    10741 agggcaacaa atcatcaatc ccggtacgat tggtatgccc tattttaatt gggaggtgtt
    10801 aaaaaatcac cgttcccagt atgccgtgat agaggttgaa gatggggaat tggtaaatat
    10861 cctatttcgt aaagtcgctt atgactacga agcggagttg aaattggcca agtccaaggg
    10921 gcttcccttt atcgaaatgt atgaagaact gcgtcgtgac gataactatc aggggcacaa
    10981 tctggaatta ttagcaagct taatagaaaa gcataggtat gtagaggatg tgaagaattt
    11041 ttttgatttt ttgtaagagt ttcctaaaat agccaatgca aactaaaaaa gcgattggct
    11101 gatcccatcg cttttaatat atcttatact caatgaaaat taaagagcaa actaggaagc
    11161 tagccgtagg ttgctcaaag cacagctttg aggttgcaga taaagctgac gtggtttgaa
    11221 gagattttcg aagagtatta ttgtaactga gattgatctg ggaggtaaga accacctaga
    11281 taggtattgc tgagtttttc aagggttccg tcttgataga gttctttgag cgctttatca
    11341 aatttctctt taaactcttt ttggtcgctt gagaaaatga tataattgct ggggctatct
    11401 gcagaaggta aatcaacgac tgagaggtct aaaccacggt ccttgataat cttttgaacg
    11461 gataccttgt caaaaactag gaaatcaaac tctccgttag caaggtctag gattcgttta
    11521 ccaatatcct caccagaaaa atcaatagta gcgggattat cagtgtgttt ctgattccag
    11581 ttattgatga attgagcgtt agaagttccg gtatcctctt gtgttgtttt accagcgatc
    11641 tggtcaagag aagtcaaagg atttttcttg ttgctgacga ggacgagggg attgttggaa
    11701 atcggaagtg agtagaggta tctttcagcg cgctcttttg tgtaactcaa gttattggcc
    11761 gcagcctgat agtgaccaga atcaagtcct gggaagatgc tctcccaagc agttctttga
    11821 aattgaatct cgtagtcgct gagtttttca tctactgcct ttagaacttc gatatcgaag
    11881 cctgtcagat tgcctttatc ttcgtagtca aatggtggca cgtcgccagc tgtagcaagg
    11941 acgattgtct tttgagagtt agtctcttta ggtgtggctt gatttccaca ggcaactaaa
    12001 agtgggagga tggctagtaa taggctaaat tttttcatac tgtctccatt caaatgtaaa
    12061 gtacttgcta gtttatcaga aactttcccg atgaactaat atatattttt tatggctgta
    12121 ataaaaaatc ttaatcatga aaaatctctg caagctcttt caaaatatgg tagaatggaa
    12181 gcagtagaat tagaaaagga aatcgattat gaaatttctt gaattaaaca aaaaacgtca
    12241 tgcgactaag aagttcactg ataagtcagt tgatccaaaa gatgtgcgta ctgctatcga
    12301 aatcgcgact ttggctccaa gtgcccacaa cagccagcca tggaagtttg tggtggttcg
    12361 tgagaaaaat gctgaactgg caaagttagc ttatggttcc aattttgaac aggtatcatc
    12421 agcgcctgta accattgcct tgtttacaga tacggactta gccaaacgcg ctcgtaagat
    12481 tgcccgagtt ggtggtgcta acaatttctc agaagaacaa cttcaatact tcatgaaaaa
    12541 tctgccagct gagtttgccc gttacagtga gcaacaagtc agcgactacc tagcactcaa
    12601 tgcaggtttg gttgctatga acttggttct cgctcttaca gaccaaggaa ttggctccaa
    12661 tattatcctt ggttttgaca aatcaaaagt taatgaagtt ttggaaatcg cagaccgttt
    12721 ccgcccagaa ctcttgatca cagtgggtta tacagacgaa aaattggaac caagctaccg
    12781 cttgccagta gatgaaatca tcgagaaaag atagaaagaa gaaaaaatga cagcaattga
    12841 ttttacagca gaagtagaaa aacgcaaaga agacctcttg gctgacttgt ttagcctttt
    12901 ggaaatcaat tcagaacgtg atgacagcaa ggctgatgcc cagcatccat ttgggcctgg
    12961 tccagtaaaa gccttggaga aattccttga aatcgcagac cgcgatggct acccaactaa
    13021 gaatgttgat aactatgcag gacattttga gtttggtgat ggagaagaag ttctcggaat
    13081 ctttgcccat atggatgtgg tgcctgctgg tagcggttgg gacacagacc cttacacacc
    13141 aactatcaaa gatggtcgcc tttatgcgcg cggggcttcg gacgataagg gtcctacaac
    13201 agcttgttac tatggtttga aaatcatcaa agaattgggt cttccaactt ctaagaaagt
    13261 tcgcttcatc gttggaacag acgaagaatc aggctgggca gacatggact actactttga
    13321 gcacgtagga cttgccaaac cagatttcgg tttctcacca gatgctgaat ttccaatcat
    13381 caatggtgaa aaaggaaata tcacggaata cctccacttt gcaggagaaa atacaggtgt
    13441 tgcccgtctt cacagcttta caggtggttt acgtgaaaat atggtaccag aatcagcaac
    13501 agcagtcgtt tcaggtgact tggctgactt gcaagctaaa ctagatgcct ttgttgcaga
    13561 acacaaactt agaggagaac tccaagaaga agctggcaaa tacaaggtga cgatcattgg
    13621 taaatcagcc cacggtgcta tgcctgcttc aggtgtcaat ggcgcaactt accttgccct
    13681 cttcctcagc cagtttggct ttgctggtcc agccaaagac taccttgaca tcgcaggtaa
    13741 aattctcttg aacgatcatg agggtgaaaa tcttaagatt gctcatgtgg atgaaaagat
    13801 gggtgctctt tctatgaatg ccggcgtctt ccgcttcgat gaaacaagtg ctgataatac
    13861 cattgccctc aacatccgct atccaaaagg aacaagtcca gaacaaatca agtcaatcct
    13921 tgaaaacttg ccagttgttt ctgttagcct gtctgaacac ggtcacacgc ctcactatgt
    13981 gccaatggaa gatccacttg tgcaaacctt gttgaatgtc tatgaaaaac aaactggcct
    14041 taaaggtcat gaacaagtca tcggtggtgg aacctttggt cgcttgctag aacgcggagt
    14101 tgcctacggt gctatgttcc cagactcgat tgataccatg caccaagcca atgaatttat
    14161 cgccttggat gatcttttcc gagcagcagc aatttatgcc gaagctattt acgaattgat
    14221 caaataaaac gatagaagtc tgagatctta tgcttggact tctttttgga gggaaagtag
    14281 atgtctcaaa tcgaaagaat caaacaggct atcatggcgg attcacagaa tgccagctat
    14341 acagagcgtg gcattgagcc tctctttgca gcgccaaaaa ctgctcgcat caatatcatc
    14401 ggtcaggctc cgggacttaa aactcaagaa gcaggccttt actggaaaga taaaagtggt
    14461 gaccgcttgc gggactggct aggtgtggat gaagatacct tttacaattc aggttatttt
    14521 gctgttttgc ctatggattt ctactttcca ggacatggca agtcgggtga tcttccgcct
    14581 cgtacaggtt ttgcagaaaa atggcatccg cagctcttac aggaattgcc tgatattcag
    14641 ttaaccctct tgattgggca atatgcccaa gcctactatt tacaggagaa aatcagtggg
    14701 aaggtaacgg agagggtgaa acactataaa gactatctgc cagcctattt tccgctagtt
    14761 cacccatcac cacgaaatca aatctggatg gccaaaaatc cttggtttga ggcagaagta
    14821 gtgccagatt tgaaaaaaag aattaaaacc attttatagt caatgaaaat caaagagcaa
    14881 actaggaagc tagtcgtagg ctgctcaaag tacagctttg aggttgcaga taaaactgac
    14941 gaagtcggta acatacgcac ggtaaggcga cgctgacgtg gtttgaagag attttcgaag
    15001 agtattagaa gaaaaagaat gaaagaaata gcctttgacg cattttacca gctttaccaa
    15061 aacgaccagc tttctctagt ggatgtgaga gaagtggatg agtttgcagc tcttcattta
    15121 gaatgtgccc acaacctacc gcttagtcaa ttggctgata gttatgatta attggacaaa
    15181 gatcgcttgc attatattat ttgcaaatct ggaatgagat cggcgcgtgc ttgccaattc
    15241 ctgttagaac aaggttataa tgttatcaat gtccagggtg gcatgttagt ctttgaagaa
    15301 ctttaaaatt ttgcatttct cctacttggt gtggactggg taggagagtt ttatttttag
    15361 ataattctta tttttaagaa aattgaaaac atttaatatt tgcctcgtga tacttttttc
    15421 agactcctaa tcgtggtata ctaggtcagt attttataaa tatgaaggag atttttatgg
    15481 ctaaaaaagg taccctaaca ggtttgctcc tgtttggaat attttttggt gcggggaact
    15541 tgatttttcc gccttctcta ggtgctctat ctggagaaca ttttcttcct gccatcgcag
    15601 gttttgtctt ttcaggcgtt ggtatcgccg tcttgaccct tattattgga acgctaaatc
    15661 ctaaaggata tatctacgag atttcaacga agatagcgcc ttggtttgcg actctttacc
    15721 tctcagttct ttacttgtca atcggtccat tctttgctac cccacgtact gctacaacag
    15781 cttacgaagt agggattagc ccccttttgt cggatgcaaa taaaggactt ggcttgattg
    15841 tatttacggt tctgtatttt gcggcagcct atttgatttc gcttaatcca tcaaaaatct
    15901 tagaccgcat tggacgtatt ttaacgccag tctttgcaat tttgattgtt atcttggtcg
    15961 ttctgggagc tatcaaatat ggtggaacaa gtcctcaagc tgcttcagct gcttatcaag
    16021 cttctgcctt tggtacaggt ttcctagaag gttataatac cttggacgcc cttgcctcag
    16081 tggcctttag cgtaatcgca gttcaaacct tgaaacaact tggattttca agtaagaaag
    16141 aatacatttc aactatttgg gttgttggta tcgttgttgc ccttgccttc agcgctcttt
    16201 acatcggttt aggttttctt ggaaatcatt tcccagtacc agctgaagcg atgaagggtg
    16261 gaacaccagg tgtttacatc ttgtcacaag ccactcaaga aatctttggc tcaacagctc
    16321 aactcttcct tgcagctatg gttaccgtaa cctgcttcac aacgactgtt ggtttgattg
    16381 tgtcaacagc tgagttcttt aatgagcgct tcccacaaat cagctacaag gtttatgcga
    16441 cagcctttac cttgattgga tttgctattg ccaatttggg tcttgatgcg attatcaagt
    16501 actcaattcc agtactggtt atcttgtacc caatcacgat tgctatcgtt atgattgtca
    16561 ttgtcaacaa atttgttgcc ctttcaaaac caggtatgca gttgacaatt gctgtggtta
    16621 cagttattgc cattgcaagc gtactaggaa gctcgtttaa ggttgagttt cttgcaaatc
    16681 ttgttagcgt tcttcctttt gccaaggcat ctctcccatg gttggtgcca gccattgttg
    16741 gaatcttgct ctcattggtt ctaccaaaca agcaagaaag cgatgttttt gaaatggaat
    16801 aatcacttaa atcacttttg tagccaagtc tacaggagtg attttctttt tttatccgat
    16861 gataaatgtg ttataatagg tagcgaaaga ggtgaagaaa tgaatcaaac agtagaatat
    16921 atcaaagaac tgacagccat cgcatcgcca acgggcttca ctcgtgaaat ttcgaattat
    16981 ttagtcaaga ctctagaagg ttttggttac cagccagttc gcacagccaa gggtggtgtc
    17041 aatgtgacca tcaaaggtca aaatgatgag caacatcgct atgtgactgc ccatgtggac
    17101 acgctgggtg ctattgttcg tgctgtcaaa ccagatggtc gtcttaaatt ggaccgtatc
    17161 ggtggttttc cttggaacat gattgaaggt gaaaactgta ccgttcatgt ggctagcaca
    17221 ggtcaaaagg tatcaggaac catcctcatc caccaaactt cttgccatgt ctacaaggat
    17281 gcaggaactg cagaacgcac gcaggacaat atggaagtgc gtttggatgt aaaagtaagc
    17341 aatgaaaaag aaactcgtgc tcttgggatt gaggtcggtg attttatcag cttcgatcca
    17401 cgaactgttg tgactgatac aggttttatc aagtctcgtc atttggatga taaggtcagc
    17461 gcagcgattt tgctcaacct gcttcgtatt tataagaaag agaagattga attgccagta
    17521 acaactcatt ttgctttttc agtttttgaa gaagtaggac atggtgctaa ttctaacatt
    17581 cctgctcagg tagtagaata tctggctgtg gatatgggag ctatgggaga tgaccagcag
    17641 acagatgagt acacagtatc tatctgtgtc aaggatgctt caggtcctta tcactatgac
    17701 ttccgtcaac acttggttgc cttggctaaa gagcaagata ttccttttaa gctggacatc
    17761 tatccatttt atggttcgga tgcttcagcg gctatgtctg caggggcaga ggtcaagcat
    17821 gcccttcttg gtgcgggtat cgagtctagt cattcttatg agcgcactca tattgactcg
    17881 gtggtcgcaa cagagcgtat ggttgatgct tacattaaga gcgcgttggt agactaatat
    17941 gtgtcttata tgccagagaa ttgagctcat caagaaggga gaaaatccct attttgtcaa
    18001 agagttggaa acaggctatc ttgtgattgg agatcaccag tattttgcag gctatagtct
    18061 ctttctagcc aaggaacacg tcacggaatt gcaccattta gaaaaggaaa caagactacg
    18121 ttttctcgaa gaaatgagtc tagtccaaga ggcagttgct aaggccttta ctgctgagaa
    18181 aatgaatatc gaactgctag gaaatggcga tgcccatctt cattggcatc tgtttcctag
    18241 acgaacaggt gacatgaatg gtcacggtct caatggacgt ggtccagtct ggtgggttcc
    18301 ttttgaagaa atgacagcag aaacctgcca agcaaaatcg gatgagatta aacaattagt
    18361 caaacgttta tcgtcagaac tagataaact attagaaata aaggagtaga aatgaaaaaa
    18421 agataccttg tcttgacagc cttgctaacc ttgagtctag cagcttgttc acaagaaaaa
    18481 gcaaaaaatg aagacggaga agctaagaca gaacagacag ccaaaactga tggaacagtc
    18541 ggaagtaagt ctcaaggaac tgcccagaag aaagcagaag tggttaacaa aggagactat
    18601 tacagcattc aagggaaata cgatgaaatc atcgtagcca acaaacacta tccattgtct
    18661 aaagactata atccagggga aaatccaaca gccaaggctg agttggttaa actcatcaaa
    18721 gcgatgcaag aggcaggttt cccaatcagt gaccattaca gtggctttag aagttatgaa
    18781 actcagacca agctctacca agattatgtc aatcaagatg ggaaagaagc tgctgaccgt
    18841 tattctgctc gacctggcta tagcgaacac caaacaggct tggcctttga tgtgattggg
    18901 actgatggtg atttggtgac agaagaaaaa gcagcccaat ggctcttgga ccatgctgct
    18961 gattatggct ttgttgtccg ttatctcaaa ggcaaggaaa aggaaacagg ctatatggct
    19021 gaggaatggc acttgcgtta tgtcggaaaa gaagcaaaag aaattgctga gagtggtctc
    19081 agtttggaag aatactatgg ctttgaaggc ggagactacg tcgattaata ctcttcgaaa
    19141 atctcttcag accacgtcag cgtcgcctta cctactgact gcgtcggttc tattcacaac
    19201 ctcaaaacag tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
    19261 tgagtacaaa aaataaactt ttctcttgca attccagata aatagtgtat aatggatggg
    19321 tatgtgaaaa acatacttgt gggaggtaaa aatctctaat taccgccaaa accacaaagg
    19381 aggatttaaa aatggctaaa aaagtcgaaa aacttgtaaa attgcaaatc cctgctggta
    19441 aagctacacc agctccaccg gttggacctg ctcttggtca agctggtatc aacatcatgg
    19501 gattcacaaa agagttcaac gctcgtacag ctgaccaagc tggtatgatc attccagttg
    19561 ttatctcagt ttacgaagat aaatcattta ctttcgttac gaaaacacca ccagctgctg
    19621 ttcttttgaa aaaagctgca ggtgttgaaa aaggatcagg tacacctaat aaaactaaag
    19681 ttgctacagt tactcgtgca caagtacaag aaattgcaga aactaagatg ccagatttga
    19741 acgcagcaaa cgtagagtct gcaatgcgta tgatcgaagg tactgctcgt tctatgggat
    19801 tcactgttgt tgactaatca ataacacagt agaaaatctc acagcagtct attagttgct
    19861 tttcatacta ggcaagtgac tgagccttgt acttgggtac agcaagggag cttaaggccg
    19921 taggaagaga aaaatagtag actgaaaacc cgcaagactt catcatttcg agaagtgacg
    19981 tgggagatga aaatcgattg aaccacttac aaggagaata gaaaatggct aaaaaaagca
    20041 aacaacttcg tgctgctctt gagaaaatcg acagcacaaa agcatacagc gtagaagaag
    20101 ctgtagcact tgcaaaagaa actaactttg caaaatttga cgcaactgta gaagttgctt
    20161 acaacttgaa catcgacgtt aaaaaagctg accaacaaat ccgtggagca atggtattgc
    20221 caaacggtac tggtaaaact tcacgtgttc ttgttttcgc acgtggtgca aaagctgaag
    20281 aagcaaaagc tgctggtgca gactttgttg gtgaagatga ccttgttgct aaaatcaacg
    20341 acggttggtt ggacttcgac gtagttatcg ctacacctga tatgatggct cttgttggac
    20401 gtcttggacg tgtccttgga ccacgtaact tgatgccaaa ccctaaaact ggtactgtaa
    20461 caatggatgt tgctaaagca gttgaagagt ctaaaggtgg taaaatcact taccgtgctg
    20521 accgtgcagg taatgttcaa gcaatcatcg gtaaagtatc atttgaagct gaaaaattgg
    20581 ttgaaaactt taaagctttc aacgaaacaa tccaaaaagc aaaaccagct acagctaaag
    20641 gaacttacgt aacaaacttg actatcacaa ctactcaagg tgttggtatc aaagttgacg
    20701 taaactcact ttaatcagta gttaaagtaa tgtaaaaaag ttgaagacgc tatgtctcaa
    20761 ctttttttga tgtacgacgg gcatgtcgta tatctgaggt gtaagtcctc ggtgggcacc
    20821 tactaccggt gaacccaata gcgattccca agtctgacta tcgtgaggta gcggattaaa
    20881 acggtctggg gatagaccgt tttaagtctg acgctggaaa taagaatcgt cagaggaagg
    20941 gatagcgaaa tcgtggctct acgaacagga acgtgataat aaggcctata tagcggataa
    21001 ggtatctggc tggggcgact actatcaatt agtagctcag aagtcggtac ttaaacgtgc
    21061 tatatcaaaa ccagtccttg aaaaacgtgg actggtttcg tgtttggatt attaccttga
    21121 acgacatgcg ttaaaagtta gttgaaccgc cgtatgccga acggcacgta cggtggtgtg
    21181 agaggggcta gagattatcc cctactcgat ttcgaaatct agtggaatga atctggaata
    21241 gtccatcgag ctttctaata ctctttgaaa atctcttcaa accacgtcaa cgtcgccttg
    21301 ccgtgtgtat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag
    21361 ctgactacgt cagttctatc cataacttca aaacagtgtt ttgagcaacc tgcggctagt
    21421 ttcctagtct gctctttggt tttcattgag tataacagat tgttagaagt tggtttaaat
    21481 tccctaatca gtttgttcac atttaccttc gatatattat atcccatagt taaggttggt
    21541 catacagatg attatagtca tggagccgta aaacttagtg tttctttagt tgacaaagat
    21601 gccatgaaaa aaatatctgt aactgtaata ggatattttg aaataaatat agatgaaaat
    21661 atcaccgata ttctatacgt aaatggtact gctattcttt atcattattt acgttctatt
    21721 gtttcaatag tttcggcaat tgatagcagt gaagcaatgt tgctacctac cattaatgtt
    21781 ttagagttac tagataaatc tcaacctttt gaagaagaat aatttattag ctcactaaat
    21841 tgagggtaag gaaaagtaaa atcagtaaga aaaatgtctt gcattataca gcaacctttt
    21901 gggaatgagt agatggattg aataaaattt tattaagagt ggatgattta tctgtagatt
    21961 attattggac agttagtctt gaagtagtct aagaattagg ttataatcag tagaatcctt
    22021 gctaataatg aggag
//
LOCUS       DAVLFB010000028        20846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLFB010000028 DAVLFB010000000
VERSION     DAVLFB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.028"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            154..3255
                     /locus_tag="VXD58_000947"
     CDS             154..3255
                     /locus_tag="VXD58_000947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV0675675.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVSYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3338..4345
                     /gene="pfkA"
                     /locus_tag="VXD58_000948"
     CDS             3338..4345
                     /gene="pfkA"
                     /locus_tag="VXD58_000948"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV0675676.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4404..5909
                     /gene="pyk"
                     /locus_tag="VXD58_000949"
     CDS             4404..5909
                     /gene="pyk"
                     /locus_tag="VXD58_000949"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV0675677.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6644..6869)
                     /locus_tag="VXD58_000950"
                     /pseudo
     CDS             complement(6644..6869)
                     /locus_tag="VXD58_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7717..8589
                     /locus_tag="VXD58_000951"
     CDS             7717..8589
                     /locus_tag="VXD58_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV0675678.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8735..9079)
                     /locus_tag="VXD58_000952"
                     /pseudo
     CDS             complement(8735..9079)
                     /locus_tag="VXD58_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9222..9534
                     /locus_tag="VXD58_000953"
                     /pseudo
     CDS             9222..9534
                     /locus_tag="VXD58_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9538..10254
                     /locus_tag="VXD58_000954"
                     /pseudo
     CDS             9538..10254
                     /locus_tag="VXD58_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10384..11199)
                     /locus_tag="VXD58_000955"
     CDS             complement(10384..11199)
                     /locus_tag="VXD58_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV0675679.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11196..12101)
                     /locus_tag="VXD58_000956"
     CDS             complement(11196..12101)
                     /locus_tag="VXD58_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV0675680.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(12347..12487)
                     /locus_tag="VXD58_000957"
     CDS             complement(12347..12487)
                     /locus_tag="VXD58_000957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675681.1"
                     /translation="MDKTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNRTTVR
                     RS"
     gene            complement(12476..12559)
                     /locus_tag="VXD58_000958"
                     /pseudo
     CDS             complement(12476..12559)
                     /locus_tag="VXD58_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12603..14732
                     /locus_tag="VXD58_000959"
     CDS             12603..14732
                     /locus_tag="VXD58_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV0675682.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            14719..15168
                     /locus_tag="VXD58_000960"
     CDS             14719..15168
                     /locus_tag="VXD58_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV0675683.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            15227..15499
                     /locus_tag="VXD58_000961"
     CDS             15227..15499
                     /locus_tag="VXD58_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV0675684.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15853..16044)
                     /locus_tag="VXD58_000962"
     CDS             complement(15853..16044)
                     /locus_tag="VXD58_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675685.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16472..17230
                     /locus_tag="VXD58_000963"
     CDS             16472..17230
                     /locus_tag="VXD58_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675686.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17232..19220
                     /locus_tag="VXD58_000964"
     CDS             17232..19220
                     /locus_tag="VXD58_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0675687.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19262..19906)
                     /locus_tag="VXD58_000965"
     CDS             complement(19262..19906)
                     /locus_tag="VXD58_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV0675688.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19932..20846)
                     /locus_tag="VXD58_000966"
                     /pseudo
     CDS             complement(19932..20846)
                     /locus_tag="VXD58_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         6348 a         3826 c         4395 g         6277 t
ORIGIN      
        1 ttttatatac aatatagtat aaatataaaa atgatgacaa aaatacaaat gaatagaaaa
       61 taaattagta agctgatgaa atttttctca agagaagcca tttataggtg aaaatggtat
      121 aatatagtga gaaggataga ggagaagtgt aaattgatcg cacaactaga tacaaaaaca
      181 gtctatagtt ttatggaaag cgtcatttcg atcgaaaagt atgtgagagc agctaaagaa
      241 tacggctaca ctcatttggc tatgatggat attgacaatc tttatggcgc tttcgacttt
      301 ctagagatta caaaaaaata cggcattcat cctttgctag ggcttgaaat gacagtgttt
      361 gtagatgatc aggaagtgaa tttgcgcttt ttagctctat ctagtgtggg ctatcagcag
      421 ttgatgaagc tttcgacagc caagatgcag ggggagaaaa cttggtcagt cctgtcccag
      481 tacctggagg atatcgcggt cattgtgtct tattttgata gagttgagtc gttagaacta
      541 ggctgtgatt actatatagg ggtttatcca gaaacactag caagcgaatt tcatcatcct
      601 atcttacctc tttatcgggt caacgctttt gaaagcaggg atagagaagt tcttcaagtt
      661 ttaacagcga ttaaagaaaa tctaccgctc agagaagttc ccttgcgttc gagacaagat
      721 gtctttatat cagcaagttc tttagagaaa ctattccaag agcgttttcc gcaagctttg
      781 gacaatttag aaaagcttat ttcaggcatt tcttacgact tggatactag tctgaaactg
      841 cctcgtttta atccagctag accagcagta gaggagttga gagagcgtgc tgaactgggg
      901 cttgttcaga aggggttgac tagtaaagaa tatcaagata gactagacca agaattgtct
      961 gttattcatg atatgggctt tgatgattat ttcttggttg tttgggattt gttgcgtttt
     1021 ggacgatcga atggctatta tatgggaatg ggaaggggtt ctgcagtagg cagtttggtt
     1081 tcttatgcct tagacatcac ggggattgac ccagtagaga aaaatctgat ttttgaacgc
     1141 tttcttaatc gtgaacgcta taccatgcct gatattgata ttgatatccc agatatttat
     1201 cgtccagatt ttatcagata tgttggtaat aaatatggta gtaaacatgc ggcacaaatc
     1261 gttacttttt caacctttgg agccaagcaa gctcttcgag atgtcttgaa acgctttggt
     1321 gtgccagagt atgaattatc tgcaattact aagaaaatca gttttcgtga caatcttaag
     1381 tcggcctatg agggcaatct ccagtttcgt cagcaaatca atagtaagtt agaataccaa
     1441 aaagcttttg agattgcttg caagatagag ggctatccaa ggcaaacctc tgtccatgcg
     1501 gctggtgttg taattagtga ccaagattta accaactaca ttcctctaaa gtatggtgat
     1561 gaaattccac tgactcagta tgatgctcat ggagttgagg ctagcggact tttgaagatg
     1621 gactttctgg gactacgaaa tttgaccttt gtccagaaga tgcaagagtt gcttgctgaa
     1681 acagaaggta ttcatctgaa aattgaagaa atcgatttag aagacaaaga aacgttagct
     1741 ttatttgcct ctggtaatac aaaaggtatc tttcaatttg agcaaccagg tgccattcgt
     1801 ctgcttaagc gtgtgcaacc agtctgtttt gaagatgtcg tcgcgactac ttctctaaat
     1861 cgaccaggtg caagtgacta catcaataat tttgtggcaa ggaagcatgg gcaggaagaa
     1921 gtgactgttc tagattcagc tctggaggat attttggctc caacttatgg cattatgctc
     1981 tatcaggagc aggttatgca ggttgctcag cgatttgctg gatttagtct tgggaaggcc
     2041 gatattttgc gtcgagctat gggtaaaaag gatgcctctg ctatgcatga gatgagggct
     2101 tcctttattc aaggctccat agaagcaggc catactgcgg aaaaagcaga gcaggttttt
     2161 gatgttatgg agaagtttgc aggttatgga tttaatagat ctcatgccta cgcctactca
     2221 gccttggcct tccagttggc ttatttcaaa acgcattatc cagccatttt ttatcaggtc
     2281 atgttaaatt attccaacag tgattactta atagatgcac ttgaagcagg ttttgaagta
     2341 gcctctctat ccatcaatac tattccctat cacgataaaa ttgccaacaa gtctatctat
     2401 ataggtttga aatcgattaa ggggctcagc aaggacttgg cgctttggat tattgaacat
     2461 agaccttatt ctaacattga agattttata gctaaattac ctgagaatta tctgaaactt
     2521 cctctgctag aacctttggt aaaagttggt cttttcgatt catttgaaaa aaatcgtcaa
     2581 aaagtattta ataacttagc taatctattt gaatttgtga aagagttggg aagtttgttt
     2641 ggagatgcta tttatagttg gcaggaatcg gaagattgga cggaacaaga aaaattttat
     2701 atggaacaag agcttttagg gataggtgtc agcaaacatc cactacaagc tattgcaagt
     2761 aaggctattt acccgattac cccaatcgga aatttgtcag aaaatagcta tgctattatt
     2821 ttggttgaag ttcagaaaat aaaagtgatt cgtaccaaaa agggtgaaaa tatggccttc
     2881 ttacaggcag atgatagtaa gaaaaaattg gatgtcactc tcttttcaga cttatatcgt
     2941 caggttggac aggaaataaa agagggagcc ttctactatg taaaaggaaa aatacaatca
     3001 cgtgatggcc gtctgcaaat gattgcacaa gaaataagag aagcagttgc tgaacgcttt
     3061 tggatacagg tgaaaaatca tgaatcggat caagaaattt cacgcatttt agaacaattt
     3121 aaaggcccaa tcccagtcat catccggtat gaagaggaac agaaaaccat cgtttctccc
     3181 catcattttg tagctaaatc caatgaatta gaggagaaat tgaatgaaat cgttatgaaa
     3241 acgatttatc gctaaaaata cggaaaatag aagaatttta aaatcaaatg tgatataatc
     3301 aagaagaatg ttaaaagaaa aaggagcata aaccaatatg aaacgtattg ctgttttgac
     3361 tagtggcgga gacgcacctg gtatgaacgc tgccatccgt gcagttgttc gtcaagcaat
     3421 ttcagaagga atggaagtgt ttggtatcta tgacggatat gctggtatgg ttgccggtga
     3481 aattcatccc ctagatgcag cttcagtagg ggacatcatt tctcgtggtg gtacttttct
     3541 tcactcagct cgttatccag agttcgctca acttgaaggg caacttaaag ggattgagca
     3601 attgaaaaaa cacggaattg aaggtgtagt tgttatcggt ggtgacggat cttaccacgg
     3661 cgctatgcgt ttgactgaac atggcttccc agctattggt cttccaggta caatcgataa
     3721 cgatatcgtt ggtactgact ttacaatcgg ttttgacaca gcggttacta ctgccatgga
     3781 cgctatcgat aagattcgtg atacatcatc aagtcaccgt cgtacttttg taatcgaagt
     3841 tatgggacgt aacgctggtg atatcgctct ttgggctggt attgcaactg gtgctgatga
     3901 aatcatcatc cctgaagcag gcttcaagat ggaagatatc gtagcaagca tcaaagctgg
     3961 ttatgaatgt ggtaaaaaac acaatatcat cgtcttagct gaaggtgtga tgtcagcggc
     4021 tgaatttggt caaaaactta aagaagctgg agatacaagc gaccttcgtg taacagaact
     4081 tggacatatt caacgtggtg gttctccaac tgcgcgtgac cgtgttttgg cgtcacgtat
     4141 gggtgcacat gctgttaaac ttcttaaaga aggtatcggt ggtgttgcgg ttggtattcg
     4201 taacgaaaaa atggttgaaa atccaattct tggtactgca gaagaagggg cattgtttag
     4261 ccttactgca gaaggtaaga ttgtggttaa caacccgcac aaagccgata ttgagctatc
     4321 tagcttgaat aagagcttgt cataatttac aatttagtta ataagaccaa aaaggtcata
     4381 tatataaagg agtcacaaaa atcatgaaca aacgtgtaaa gatcgttgca actttgggtc
     4441 ctgcggtaga aatccgtggt ggtaaaaaat tcggtgagga cggatactgg ggtgaaaaac
     4501 ttgatgttga agcttcagct aaaaacattg ctaaattgat tgaagctggt gctaacacat
     4561 tccgattcaa cttctcacac ggcgaccacc aagaacaagg tgagcgtatg gcaactgtta
     4621 aacttgcgga aaaaattgca ggtaaaaaag ttggtttcct tcttgataca aaaggacctg
     4681 aaatccgtac agaattgttc gaaggtgaag ctaaagaata ttcatacaaa actggtgaaa
     4741 aaattcgtgt tgcaactaaa caaggaatca aatcaactcg tgaagtgatt gcgttgaacg
     4801 ttgctggtgc tcttgatatc tatgatgatg ttgaagttgg tcgtcaagtt ttggttgacg
     4861 atggtaaact tggtcttcgt gtggttgcta aagatgatgc aactcgtgaa tttgaagttg
     4921 aagttgaaaa cgatggtatc atcgctaaac aaaaaggtgt gaacatccct aacactaaaa
     4981 ttcctttccc agctcttgct gaacgcgata acgacgatat ccgtttcggt cttgaacaag
     5041 gtatcaactt catcgcaatt tcattcgtac gtactgcaaa agatgtgaac gaagttcgtg
     5101 caatctgtga agaaactgga aacggacatg ttcaattgtt cgctaaaatc gaaaaccaac
     5161 aaggtatcga taacttagat gaaatcatcg aagcagctga tggtattatg attgctcgtg
     5221 gtgatatggg tatcgaagta ccgttcgaaa tggttccagt ttatcaaaaa atgattatca
     5281 agaaagtcaa tgctgcaggt aaagttgtta tcactgcaac aaacatgctt gaaacaatga
     5341 ctgaaaaacc acgtgcaact cgttcagaag tatcagacgt attcaacgct gttatcgacg
     5401 gaactgacgc tacaatgttg tcaggcgagt ctgcaaacgg taaataccca ctcgagtcag
     5461 taactacaat ggctacaatc gacaagaacg ctcaagctct tcttaatgaa tacggacgtc
     5521 ttgattcaga ttcatttgag cgtaactcta agacagaagt aatggcttct gctgttaaag
     5581 atgctactag ctcaatggat atcaaattgg ttgtaactct tactaagaca ggtcatactg
     5641 cacgtttgat ttctaaatac cgtccaaatg ctgacatctt agcattgaca tttgacgaat
     5701 tgacagaacg tggcttgatg ttgaactggg gtgttatccc aatgttgaca gatgctccat
     5761 cttcaactga cgatatgttc gaaatcgctg aacgtaaagc ggtagaagca ggtctcgttg
     5821 agtcaggcga tgatatcgtt atcgttgctg gtgtgccagt aggagaagct gttcgcacaa
     5881 acacaatgcg tatccgcaca gtacgttaag aaaaatataa aaacctatca tatccagctt
     5941 tctagcctgt atgatgggct ctttttttgt ccagggggca aaaagggggc aaaactttca
     6001 taatggattg atactcttcg aaaatcaaat tcaaaccacg tcagcgtcgc cttaccgtat
     6061 atatgttact gacttcgtca gttctatcta caacctcaaa gcagtgcttt gagcaacctg
     6121 cggctagctt cctagtttgc tctttgattt tcattgagta tgaaataaga tatgcataaa
     6181 ttgattagaa agtcaaatga atttctacaa atgttttagc aatcgcaatc tacttgtcta
     6241 gattcgatct gatatatttt cgatttaatg atatggtatt taaaacctcc aaagtagctt
     6301 gctccattct tttacctacg tgagtctaga tgttatttac tgttttagcg tttttgtgtt
     6361 ctactctaac cattatagca ttcttctcag ctagtgtact aaggagtgtg tgcctgaaaa
     6421 tatgggaact aaggggctgg tttatcggtt tctctagttt agtatttgcc ttttgcaaag
     6481 tgatcttaaa tgcctttctc taaatttaca tatcactatt gtttaacaaa atctaatcta
     6541 ttttaggtca cttattcttt ttttgaaatg tagaatgaac tttttcaaag tttttcgaat
     6601 cttttaaaat ctgtttgttt tatatcgcca ttctccccct tttttaattc tccctatata
     6661 gcctgacaac ttttccgatg gtacgaatat ggttgctttc gtctaggtgg atgtcggagt
     6721 attcgggatt gagttttttt gaggtagcct tggcggagtt tcttgacata gttagtgccg
     6781 tctacttgga agatgccgat ggtattatag tcaatctgtg gggtattctt gataaatagg
     6841 tagtcgctgt ttcttatctt tggctccatg gacttgctga cgacataagc gattgggtcg
     6901 tagtcgtctg ggataatgga aactccataa ctaaatcgtc gtcctgcatc aagcggctac
     6961 ctgcagagat aaactaccta acacgagagt aagtagtctg tctgtagtcg tccagtctga
     7021 tgatttttac gatacttcgt ttttctgatc atacagttgc ctctcggcat aggtcagaac
     7081 tttaccttgt ctgggtggtg cccgttggtc gtagatagat tggatatcgc taggagaatc
     7141 cttttgaact ggaggaaaga gggcatcaat caagctactg aatactttaa ctaagtcaaa
     7201 tatagtattt ttcttagtag acctaaccct tttttcataa tttctaatgg tgtttttact
     7261 tatacctatc ttagtaccca attcttattg agtccaacca ttactagtct atattgtttt
     7321 atagttgatt gagtttggaa tagtacgctg tagctgctaa aacatttcta ggaattaatt
     7381 tgcctttcct aatagagttg ttcatatctt atttcaatct actatatttt tcacctctaa
     7441 caatcgcaat ctcttcttta tccatgaacg aaaacgcttt ctatctttgt aagtaaagca
     7501 tcacacgaaa tccacgaaaa tgaaaacctt tgttgtgttt tcgtaaaaaa tttgttgaca
     7561 gagcacgaaa cgcctactat aattaaatca agtttaagga aataacaaaa aaacaaatcg
     7621 gagggaaaga cgatgaacac attaaatgag aaagtaatca atatctgtaa agcagtagtt
     7681 aaagaaactt taatccaaga catttaggag aaagaaatga agaaatcaag aaaattagcc
     7741 acattaggaa tctgttcagc cttgttttta ggcttggctg cttgtcaaca acaacatgct
     7801 acttctgagg ggacgaatca aaggcaaagc agttcagcga aagttccatg gaaagcttca
     7861 tacaccaacc taaacaacca ggtaagtaca gaagaggtca aatctctctt atcagctcac
     7921 ttggatccaa atagtgttga tgcatttttt aatctcgtta atgactataa taccattgtc
     7981 ggctcaactg gcttatcagg agatttcact tcctttactc acaccgaata cgatgttgag
     8041 aaaatcagtc atctctggaa tcaaaagaag ggcgatttcg ttgggaccaa ctgccgtatc
     8101 aatagttatt gtcttttgaa aaattcagtc accattccaa agcttgaaaa gaatgaccag
     8161 ttgcttttcc tagataatga tgcgattgat aaaggaaagg tctttgattc acaagataag
     8221 gaagagtttg atattctatt ttcgagagtt ccaactgagg caactacaga tgtcaaggtt
     8281 cacgctgaaa agatggaaac attcttctca caatttcaat tcaatgaaaa agctcgaatg
     8341 ctgtctgtag tcttgcacga caatctggat ggcgagtatc tgtttgtagg ccacgttggg
     8401 gtcttagtac ctgctgatga cggtttctta tttgtagaga aattgacttt cgaagagccc
     8461 taccaagcga ttaaatttgc tagtaaggaa gattgctaca agtatttagg caccaagtat
     8521 gcggattata caggcgaggg actggctaag ccttttatca tggataatga taagtgggtt
     8581 aaactttaat agtgttatga tatgacagta atcttaggat ttttatttct atttttgatg
     8641 agttggtggg ttatttcaat cgttaatagc cagaaaccac gcaaaaatga aacatttatt
     8701 ggctatatcc aaagatacga tattgatggg aatggctatc aatcattacc gtgtcctcag
     8761 aactgagagg agttcttgaa atcgtaaaac tactttgaac aagagttact tcaacccatt
     8821 ggctccgacg gattaagttg ctttcgtgaa caccaaaatc agccgcaatt tcttcataag
     8881 tgcggtattc tcgcacatat tgaagagtgg ccataagaag gtcttctagg cttaatttag
     8941 gttttcgtcc cccttttgtg tgtttaagtt gataagcagt ttttaataca gctaacatct
     9001 cttcaaaagt ggtgcgctga acaccaacaa gacatttaaa tcgtgcatca gttagttgct
     9061 tacttgcttc ataattcata gaactactat accatatttt gtttcgcagg aagtctaatg
     9121 ctaaaatctt atcagtgaga agatttctta aagtttattc tttgtagttt catctgtatt
     9181 agtacataat ttaacttaga gaaaatagga ggactaatcg aatggaatta aaagatttta
     9241 cagaaaaaga acaggaaatg attaagaaaa ggcttacaat gtctaatatt agtgacaaag
     9301 aaactactga gaagattctg gcgctagtac cacaagactt gattaagcga atcccgtttt
     9361 ttgtcagaaa acatgctaca acacgtacga ttaaacgcat ttcaattgaa caccctgaac
     9421 tctacgctgt agctcaaaca agtggtgaga ttccagaaaa aaaaacgcga agaattgcgt
     9481 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
     9541 aaaaaatatt ttattggcgg tttgggaagc aatgcctatc atagcaagga ttttctttaa
     9601 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
     9661 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggt
     9721 cattctattg gaggcgattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
     9781 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
     9841 ttagaggaaa ctaaaaatta tatcaaatct caaattattt cggacttaga tgttcttact
     9901 tctaaagaaa aatctgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
     9961 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
    10021 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
    10081 ttgtttatta gtcctcgcta tcctaatgaa gctacatgga gagaggaagc cctaaaagaa
    10141 ttgccagact attttgatac tattttttta gaaaactttg gccatgagct ttatactcaa
    10201 gcacctaaag aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact
    10261 tgtattgctg gaaatggtat aagatgaaaa tctacaagtc actaattttc atataaccaa
    10321 aaaacgaaca ggctcttttt ctgttattca gaagagaact tgttcgtttt tatcttgatt
    10381 ttcttaaaaa gctctatata catagggatt ttctggttta tcgactttgg taaagctgtc
    10441 gatttccaag gttggaaggt tttgtttgag ttctttatgg tagtgattga ccagttttcc
    10501 tttggctgaa atccaagtgt tgttttcata ctgccgggta ttgcccttgg tcagcaatcc
    10561 atagacacca gaatctgcga tacagtggat aatgccgaat cggaacagaa attgactatt
    10621 ggcatgactg gggtcgttat agacaaagcc tgtaaactgg attgtcttgc cctcaaactc
    10681 atctggatag tcgtagatag cctccatgac ttccatatag ttttcattag tgatctgaat
    10741 gctgtcttgg gataagtatt tatccgccgc cgttcgcatt tccttttcat aggctgattt
    10801 tgaaaaataa gaactagtat ctggtttcaa atattggctt gtagtccctt cacttgtctg
    10861 aatagccaga ttcgttcctt ccgatagagg gaaatgatag cctttagctg aaacagtctg
    10921 agaatccaag ctaacagttg ggaaagttaa gccgatgaca agtggaatag ccagaagaga
    10981 aatactcgtc atcttggcca atcggctgtt cagatgactg tgggttttga cttgcttcat
    11041 ccagatatac aattgaacga tagccaagat aaaagaaagc accatggaga tataggccag
    11101 ataggaatag tgcatgttga tgtactggtt tagtttgccc gacagatgga ggtaaatagt
    11161 cagttcaaaa tagccagcta aaactaaaaa tcgaatcata ggatgactcc aatcaagaga
    11221 gaatagacta agacgacaag agttacaatt gttatgaagt gactgataaa tcgtgctttc
    11281 aagtaatttt tcatcatgag aatatttttg atatccagca ttggaccaat gacgagaaag
    11341 gccagaactg gtgccaaacc gaaactcgag agaagagaag cacctataaa ggcatccgcc
    11401 tcactacaga gcgaaagaag aaaggctaaa atcatcaaga gcaggatggc aaaaagaggg
    11461 gtcgcactga tagaggtcag aatccgagtc ggaacgtaga cctgtattat agaagcaaag
    11521 aggcagccaa ataccaaata acgccccgta tcaaaaaatt catcaatggc ctgcacaaag
    11581 acttgaaaaa ctttttttgc agaactcaag taagaaaaat catgctcatg acaagccaga
    11641 cgattttctt tctgaatcgg ttcttgccag aaaaatccta gaaaaattcc tagtattaca
    11701 gccacaagaa tggaacccag agctcgtaat agggcgacat ggaaggagtt gccaaaggca
    11761 gaataggtcg caaaaagaac aatgggattg ataacaggtg ctgtcacaag aaaaggaacg
    11821 gccgtgtaac ttggaacctt tttttccaga aaacgattga tgatggggac gattccacat
    11881 tcacaagaag ggaaaagtat accgacaaag gtcccaaaaa agattctccc ccaacgattt
    11941 cgagggagaa aatgataaac cttgtcaggt gtgatataaa cttcaatcag ccctgagaca
    12001 atgcttccta tcagaacgaa gggaagggct tcaatgataa tggagagaaa aatggctcca
    12061 gtttgaagta cactagatgg gagttgttga aaagcagtca tctagttttt cttttctgct
    12121 ttgacttttt tctctttgtc atcagggaaa gtaacttgtt tcaaatcagg gagtttagca
    12181 aactcttcaa acatcttgtc caagtcgtct cgttttgtaa atttgacagc cataggtgta
    12241 cctcgattct aaattctacc tctattatac tattattttg cggaaaaggg agttttttaa
    12301 tttgttttcg atagttgata ctcttcgaaa atctcttcaa accacgtcag cttcgcctta
    12361 ccgtagtacg gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
    12421 tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact tcgtcagtct
    12481 tatccacaac ctcaaaacag tattttgagc agcctacggc tagcttccta gtttgctttt
    12541 tgatttttat tgagtatgaa aaggattgga gaaagccttc cagcttgtgg tataatagat
    12601 ttatggataa aaaatatgaa aaaatctctc aggatttggg agtgacgtta aagcaaattg
    12661 ataccgttct aagtttgaca gctgaagggg cgactattcc ctttatcgcg cgttatcgca
    12721 aggacatgac tggtagtctg gatgaggtgg cgattaaggc tattattgat ttggataaaa
    12781 gtctgacaaa tctcaatgac cgtaaggaag ctgtcttagc taagattcaa gaacaaggta
    12841 agttgaccaa ggaattggaa gaagctatct tagttgccga aaaattagca gacgttgaag
    12901 aactctatct tccttataag gaaaagcgtc gtaccaaggc aaccattgcc cgtgaagctg
    12961 gactctttcc tcttgctcgt ttgattttgc agaatatagt tgacttagag aaagaagctg
    13021 aaaagttcgt ctgtgaagga tttgcgactg gcaaggaagc cttgaccggt gcagttgata
    13081 ttttggtcga agccttatcg gaagatgtga ccttgcgttc tatgacttat caggaagtgc
    13141 tgagacactc taaactcact tctcaagcca aggatgaaag tcttgatgaa aagcaggttt
    13201 ttcagattta ttatgatttt tcagagacag ttggaactat gcaaggctat cgtaccttgg
    13261 ctctcaatcg tggggagaaa cttggtgtct tgaagatcgg ttttgaacat gcgacggacc
    13321 gtattcttgc cttctttgct actcgtttca aggtgaaaaa tgcttatatt gatgaagttg
    13381 ttcagcaatc cgttaagaaa aaggtcttgc ctgctattga gcgtcgtatt cggacagaat
    13441 taactgagaa agctgaagag ggagctatcc aacttttttc tgacaatctg cgcaatctcc
    13501 tcttggttgc tccactgaaa gggcgcgtgg ttcttgggtt tgacccagcc tttcgtacag
    13561 gtgccaagct agctgtcgtg gatgcaacag gaaaaatgct gacaactcag gttatttatc
    13621 ctgttaaacc agcatcagct cgtcaaatcg aagaagccaa gaaagattta gcagatttaa
    13681 ttggtcaata cggtgtagag attattgcca ttggaaatgg aacggccagt cgtgaaagtg
    13741 aagcttttgt agcggaagtt ctgaaagatt tccctgaagt cagctatgtt atcgttaatg
    13801 aaagtggtgc ttctgtctat tctgccagcg aacttgctcg tcaggagttt ccagacttga
    13861 ccgttgaaaa acgctctgcc atttctatcg cccgtcgttt gcaagatcct cttgcggaat
    13921 tggtcaaaat cgatcctaag tcaattggtg tcggtcaata ccaacacgat gtcagtcaga
    13981 agaaactatc tgagagtctg gactttgttg tcgatacagt ggttaaccaa gttggtgtca
    14041 atgtcaatac agctagccca gctcttcttt cacacgtagc tggactcaat aaaaccattt
    14101 ctgaaaatat cgtcaaatac cgtgaggaag agggaaaaat cacttcacgc tcccaaatca
    14161 agaaagttcc tcgtttgggg gccaaggctt ttgaacaggc tgctggtttc ctccgtatcc
    14221 ctgaaagtag caatatcctt gataatacag gagttcaccc agaaaattac gctgccgtta
    14281 aggaactctt caaatgcttg gacattaagg atctgaatga agaggcccaa agcaaactca
    14341 agtcgctttc agtcaaggaa atggctcaag agctagacct cggtccagaa acccttaaag
    14401 atatcattgc agatcttctc aaaccaggtc gagatttccg tgattccttt gacgcacctg
    14461 tgcttcgcca agatgtccta gatatcaaag acctagtggt aggtcagaag ctagaaggtg
    14521 ttgtgcgtaa cgttgttgac ttcggtgcct tcgttgatat cgggattcac gaggacggct
    14581 tgattcatat ttctcatatg agtcgcaagt ttatcaaaca ccctagtcaa gtggtggcag
    14641 ttggagattt ggtgaccgtt tgggtcaaga aaattgatac tgaacgtgaa aaagttaatc
    14701 tgtcgctcct cgctccaaat gaaactgact gattatgtca agcaggtttc actagaagac
    14761 ttcgggagag cttttatcca tcatgtccag tggaataggc gtctacgttc gacaggtggg
    14821 cgatttttcc ccaaagatgg gcatctggat tttaatccca aggtttatca gaaactgggt
    14881 atggaggttt ttaggaaaat tgtccgacat gaactctgtc attatcacct ttatttccaa
    14941 gggaagggct atcaacacaa ggatcgggat tttaaggaac ttttgaaagc agtggatgga
    15001 ttacggtttg taccatcctt gcccaatagt aactctaaac cactcaagct ttatcgttgt
    15061 caatcctgcc agcaaaggta tcagcgcaag cgtaggattg ataccaaacg ctatcgctgt
    15121 ggactttgtc gaggtaaatt gcttctgata aatcagcctg aggactgatg aaagccgagc
    15181 ctccccgtga tatactatgt ctaacctagc agaaagagga aatatcatga acagtaaatt
    15241 ttataaaatg agacgcaatc gcatgatttc aggagttctg gcaggtctgt cagacaagtg
    15301 gaattttgat gttaccctag ttcgtttctt gtttgctatt tttacagttg caaactttgg
    15361 acttggcgtg attatctata tcatcctagc ttctatcatg cctaccaagg aagaaatcga
    15421 agcagaaatg tacggaacag gcccacgtaa acgcaaagaa gcccaagcca ttgacgacaa
    15481 tgaaggctgg ttttggtgag gttctacaga agatttggaa taagcaaaaa gctttaaatc
    15541 aatagtctaa actgcttgat ttaaagcttt tttactttta tactcaataa aaatcaaagt
    15601 gcaaactagg aagccagcca caggctgtaa tttagtcggc aatgtgaagt tgacatagtt
    15661 tgcactttga tttttgaata gtattaataa tatttcgcca gcttcatcca caaactatag
    15721 tggattggaa ctagaatagg acacctctac ttctaaaaca ttgttagaaa tcgatttgac
    15781 tatcctgatc gatttgtcct gttcttattt cattttatta taaaagcagt aatttaaact
    15841 cagtttttta atctattttt gctgctgaga caatacattt ttggaaaaat ggtggatgct
    15901 gataacaact gataaccagc aaataacata agcagtgccg gtaaattctg taccgataaa
    15961 gtgtgtgtgt tcaactaaga gctgaccgat gaatggagag agcagataga gaagtccgat
    16021 gagtatatgt tctttgtgtt tcattaaagc gcaccttctt tccttatagg ttgaacgact
    16081 tgcaagtgtt aaattgaatg gtatgttatc taggtaacct tactaaaatg ttagttcccg
    16141 cttcctcctt ttcaaagtaa gccttttcac acttatattt tacaacttta aaaataggaa
    16201 atcaaatcat tttctaagta attttgctta tatttcaccc aatcttacaa aaatgtaaga
    16261 ttaaagtctc ttttgtaagg ttaggttatg gcaagcggtc tttttttgat gtaaactaga
    16321 ctcataaaga acaaaggagt aacatcatga aacaaatctt acataagaaa actagaaaac
    16381 caagtcacaa agaaattgag cgcgttcagc tgggatgcac tatgatgcaa gctacatttc
    16441 atttgatggg atattaagaa aggagattct catgacactt ttagatgtaa aacacgttca
    16501 aaaaatttat aaaacacgtt ttcagggcaa ccaagtagaa gccctcaagg atattcactt
    16561 taccgtcgaa aagggtgact acgttgccat catgggtgag tctggttctg gtaaatcaac
    16621 tcttctcaat attctagcta tgctggataa accaagtcgt ggtcaggttt acttgaatgg
    16681 aactgacacc gcaaccatta aaaattcaca ggcctctagc ttccgtcgtg aaaagttggg
    16741 ctttgtcttc caagacttta acttgctaga tactctatct gtaaaggaca atatcttgct
    16801 tccgcttgtc ttgtcaagaa gacctattac agagatgatg aagaaattgg tggtgacagc
    16861 tgagaatctg ggaataaacc aattacaaga gaagtaccct tacgagattt ctggtggtca
    16921 gaaacagcgt gtagcagtag cccgcgccat catcacagaa cctgaaattc tccttgcgga
    16981 cgaaccgaca ggagctcttg actccaagtc atctgcagcc ttacttgatg tctttgatga
    17041 aatcaatgag cgcggccaaa ccattctcat ggtaacccac tcaacagcag cagctagcag
    17101 agctaaacgt gtactcttta tcaaagacgg cattctttac aatcaaatct accgtggaga
    17161 gaagacagaa cgtcagatgt tccaagaaat ctctgatacc ttgactgtca tggcaagcga
    17221 ggtgaattag tatgtttcga ttaaccaata agttagcggt atcgaacttg attaaaaacc
    17281 gcaaactcta ctatcctttt gcgctggctg ttctcttggc agtcactgtc acctatctct
    17341 tttactctct aaccttcaat cctaagattg cggaaatccg tggaggaaca accattcagg
    17401 ctacacttgg atttggtatg tttgtcgtca cccttgcgtc agccattatc gttctctatg
    17461 ccaatagttt tgtcatgaag aaccgttcca aggaactagg aatttatggc atgttgggct
    17521 tggagaagcg tcatcttatc agtatgacct ttaaggagtt agtggtattt gggattctaa
    17581 ctgttggagc gggtatcggt attggagcct tgtttgacaa gttaattttc gctttcctgc
    17641 tcaaactaat gaaattgaag gttgagctgg ttgctacctt ccagacgaaa gttgtcatta
    17701 cagtgcttgt tgtcttcggt ttgattttcc taggcctcat gttcctgaat gcccttcgaa
    17761 tcgcccgtat gaatgccctc cagctctctc gtgagaaagc tagtggagag aaaaaaggtc
    17821 gcttccttcc tctccaaacc attcttggtt ccataagttt aggaattggc tattatctcg
    17881 cccttacggt aaaagatcct cttacagcct taacaacctt cttcatagct gttttactgg
    17941 ttatctttgg gacttatctc ttgtttaatg cagggattac cgttttcctc caaatcttaa
    18001 agaaaaataa gaaatactat taccaaccaa ataacctcat atctgtttct aacttgattt
    18061 tccgtatgaa gaaaaatgca gttggactag ccaccatcgc tatcttgtca acaatggttt
    18121 tggtaaccat gtcagcagcg acaagcattt tcaattcctc agaaagcttt aaaaaagttc
    18181 taaatcctca tgattttggg gtttcagggc aaaatgttga aaaagaagac ttggacaaac
    18241 tcttgagcca gtttgcaagt gacaaaggtt ataagattaa agaaaaagaa gtatttcgtt
    18301 acacttactt tgctgttgcg aatcaagaag gaaccaagtt aactattttt gaaaaaggac
    18361 aaaaccgtgt ccaaccaaaa acagttttca tggtatttga ccaaaaagat tatgaaaata
    18421 tgactggtca aaaactgtct ctatcaggaa atgaggtcgg tctctttgcc aaaaatgacg
    18481 gactgaaagg acagaaagct ctaactctaa atgatcatca attttctgtc aaagaagaat
    18541 ttactaaaga ttttattgtc aaccatgttc caaataagtt taatatcttg actactgatt
    18601 acaattacct tgttgttcct gatttacaag cctttttgga tcaattccca gattcggcta
    18661 tctataatca gttttacggt ggtatgaatg tagatgccag cgaagaagaa caactcaagg
    18721 tcgctgagga gtatgaaaac tacctcaatc aatttaatgc tcaattagac acagaaggta
    18781 gctatgttta tggtagcaat ctagcagatg ctagttctca gatgagtgcc ctctttggtg
    18841 gtgtcttctt tatcggtatt ttcctatcca ttatctttat ggtcggaact gttctggtca
    18901 tctactacaa acaaatttct gaaggctacg aagaccgtga acgctttatt atcttgcaga
    18961 aagtcggttt ggaccaaaag caaatcaagc aaaccatcaa caaacaggtt ttaactgttt
    19021 tcttccttcc tttgctcttt gccttcatac atctcgcctt tgcctaccat atgcttagcc
    19081 tgattttaaa agtgattggt gtactggata cgactatgat gttgattgtg accttgtcta
    19141 tctgcgctat cttcctcatc gcctatgtgc tgattttcat gattacttca agaagttatc
    19201 gcaagattgt gcaaatgtaa aaaagatacc tcgacttcaa aatcgaggta tttcttgtat
    19261 tctaaatgct gaaaagttgt tcgagcagga aggtaactcc catggtcaag agaccaatag
    19321 caaggttccg aatcatagct gttttggttg gggcttttcc aagtctagca cttgtgtaac
    19381 cagtgagaag aagggccaca ccgacaataa ggacggtagc agggatgcgg taatcacttg
    19441 gaaaaatggt cactgacagc attggaggca aacttctaag gaaaaaggca acgaagctag
    19501 aaatggcagc gtgccaagga ttggtaaatt cttcatactc aatcccatat ttttcctcta
    19561 ccagagcctt gagtggattt ttaagaaaga tcttattggt caagagttgg gcagaagttt
    19621 tgaattctcc attttggata taagcagcat agagggattt tttggctagt tccctatctt
    19681 ggtctagcaa gagtttttct cgcgaaacgg cagcttcctc ggtatctttt ggagttgaaa
    19741 cggatacata ttctccacca gccattgaaa aggcaccagc taagatagcc gtaaaacctg
    19801 ataaaaagat aatccagata ttggtcgtgg cactggcaac tccgataacc acaccagcaa
    19861 tggaaataat tccatcgtta gcatcaagaa cacccgcacg caggatattt aaacgacctg
    19921 caaaatttga atcaatttcg tgatttgttt ctgacgctaa atttcaagtt caagttagcc
    19981 atccagaagt cttctctggg tgacttgtag tccaagcatt ttttaggata gttgttaatc
    20041 cacttttcga tgaatgcgac ttctttggga gtcattttct tggttccctt aggtaactat
    20101 ctacgaatga gcctgttgtg attctcatta gttccccttt cccaagaggc ataggggtgc
    20161 gcataataga tgtgctcctc agaaaatata tcaaacaagc gattgaattc cgttccatta
    20221 tctgccgtga tggaaagaat cttgtgttgt tttaagatga gttttagagc ctgattgacc
    20281 acctcagcac ttttatttgg aatcaatcgg atgatctgat gtctactctt tcgatccgtc
    20341 aagacaagca agcagtagtt tttcgatctc gtaagtagaa ccgtatcaat ctcataatgc
    20401 ccattctcca agcgaagatt gatagcttca ggccgctgtt cgatggattg accagcaggt
    20461 ttaaagttgg tgctagcctg tttcttaagc gcttttcctt ttctagggta aagcagatcc
    20521 tgcttgctta accccaattt tccatgatga atccaatagt aaatggttga aattcccacg
    20581 ttaacccctt tagccataac catcatttca ggcgaaaatt tttggttatg atagtggaga
    20641 atcttttcct ttagttcctt ggtcacgctt gatctcttga ccgagtgctt gcgattattt
    20701 tcataagact gttgagcata gtcggcagaa taaacctctt tgaagcgccc ttttccaaga
    20761 cattgtcgga ctgtcctacg cttgatttca gtgtggatag tttgaggagc ttttccaagt
    20821 agagaggcaa tttctctatt tgattt
//
LOCUS       DAVLFB010000029        19613 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLFB010000029 DAVLFB010000000
VERSION     DAVLFB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.029"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(166..780)
                     /locus_tag="VXD58_000967"
     CDS             complement(166..780)
                     /locus_tag="VXD58_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV0675689.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VXD58_000968"
     CDS             complement(1595..1864)
                     /gene="rpsO"
                     /locus_tag="VXD58_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV0675690.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2005..2634)
                     /locus_tag="VXD58_000969"
     CDS             complement(2005..2634)
                     /locus_tag="VXD58_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV0675691.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2872..3072
                     /locus_tag="VXD58_000970"
     CDS             2872..3072
                     /locus_tag="VXD58_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675692.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            3503..3826
                     /locus_tag="VXD58_000971"
     CDS             3503..3826
                     /locus_tag="VXD58_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV0675693.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3884..5827
                     /gene="thrS"
                     /locus_tag="VXD58_000972"
     CDS             3884..5827
                     /gene="thrS"
                     /locus_tag="VXD58_000972"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV0675694.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5876..6850)
                     /locus_tag="VXD58_000973"
     CDS             complement(5876..6850)
                     /locus_tag="VXD58_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0675695.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6843..7520)
                     /locus_tag="VXD58_000974"
     CDS             complement(6843..7520)
                     /locus_tag="VXD58_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675696.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7676..8749
                     /locus_tag="VXD58_000975"
     CDS             7676..8749
                     /locus_tag="VXD58_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV0675697.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8840..9142)
                     /locus_tag="VXD58_000976"
     CDS             complement(8840..9142)
                     /locus_tag="VXD58_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675698.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9210..9647)
                     /locus_tag="VXD58_000977"
     CDS             complement(9210..9647)
                     /locus_tag="VXD58_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV0675699.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9723..10565)
                     /locus_tag="VXD58_000978"
     CDS             complement(9723..10565)
                     /locus_tag="VXD58_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV0675700.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10677..11327
                     /locus_tag="VXD58_000979"
     CDS             10677..11327
                     /locus_tag="VXD58_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV0675701.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11537..12172
                     /locus_tag="VXD58_000980"
     CDS             11537..12172
                     /locus_tag="VXD58_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV0675702.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWENYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            12303..12716
                     /locus_tag="VXD58_000981"
     CDS             12303..12716
                     /locus_tag="VXD58_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675703.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            12735..13149
                     /locus_tag="VXD58_000982"
                     /pseudo
     CDS             12735..13149
                     /locus_tag="VXD58_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13188..13631)
                     /locus_tag="VXD58_000983"
     CDS             complement(13188..13631)
                     /locus_tag="VXD58_000983"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV0675704.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13661..15883)
                     /locus_tag="VXD58_000984"
     CDS             complement(13661..15883)
                     /locus_tag="VXD58_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV0675705.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16294..16536
                     /locus_tag="VXD58_000985"
     CDS             16294..16536
                     /locus_tag="VXD58_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675706.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            16876..17505
                     /locus_tag="VXD58_000986"
     CDS             16876..17505
                     /locus_tag="VXD58_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0675707.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(17628..19520)
                     /gene="pepO"
                     /locus_tag="VXD58_000987"
     CDS             complement(17628..19520)
                     /gene="pepO"
                     /locus_tag="VXD58_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV0675708.1"
                     /translation="MTRYQDDFYDAINGKWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5748 a         4447 c         3628 g         5790 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccccct gaaaatcaga
      121 gagctgattt gagctcgggc taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
      301 ccgactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaacc tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
      841 attaatttag ctatcgctag tgggtaatgt cgaacgtagg aaagcatttt actcctcctc
      901 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
      961 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacagctac
     1021 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccatttga
     1081 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
     1141 cagagagctg attttagccc gggctaaaat cctagtgaaa aagatgaaac tccttgtgct
     1201 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
     1261 attcttgcta gataaatcgt ttatagacta ggggacgagg tgaagatcgt acgaaacttt
     1321 cagtgaaaca aaaaaatctc cccgaaggga gaaaagctag tttattttac cttacagtgc
     1381 taggaaccgt aaccgaagat tatacttgag tatagcgagg taaggtgaca acgcaacaaa
     1441 gctacccaat ttagagtagc tttattatca agatatgctc aattgaacac ggcctaagca
     1501 cttggcaaaa aagataaaat ctcctagaaa ctgaagtttc ttcgtcaatt ttcctatttt
     1561 tgctttgtgt ttttgacggc ctttgtatct tgaattagcg acgaagtcct agagagttga
     1621 tcaactcacg gtaacggtta acgtcgtttt tacgcaagta tgcaagcaag ttacgacggc
     1681 gaccgatttt tttcatcaat ccacggtaag tagcgtggtc ttttttgtgt tgtttgatgt
     1741 gttcgttaag gtggttgatt tcccaagtaa ggacagcaac ttgaacctct actgaacctg
     1801 tatcaccttc gtgacgtgca tattgtgcaa tgatttcatt ttttttctct tttgagattg
     1861 ccatgatgtt tctccttttt atttggcttc atccgagtga caggttggca tgcctgtaac
     1921 caagaagaag ttatttgtct ttacgacaat ccctatttta ccaagattta aattttctgt
     1981 caaacattaa tactctcata gttattattt ttcaagtaaa gagatacgtt cttttatatg
     2041 atgacctttt tcaagttcat ctaccaatcc aatagcataa tctgcataac taatctggct
     2101 tattcctttt tcattagtag taaaaatttc tcctgctaga atgtagtttc ctgttttttc
     2161 accatcggga ataaaatcta cagcaggact gacaaaagtc cagttgagat tattttttgt
     2221 acgaagaaga tccaattcat ccgcttgagc ttttgctagt gacttaaatt cttcaggaaa
     2281 atcagacgta tctaaaagtc gtgtcgtttt tgtttcatct atataaagac ttcctgctcc
     2341 ccctacaact agaaaacgtg tctgtgttcc agcaagaagc tgattaaata gttcgattga
     2401 tttgctgtgg agcggtagcg tatctggtgt ataagcacca aacgctgaaa taacagcatc
     2461 aaatccagta agatcatctt ttgtcaactc aaataaatct tttttaataa tagactcagc
     2521 ttgacttttg ttttcagaac gaacaatagc cgttacttca tgtcctcgtt tgactacttc
     2581 ttcaacaatt gctttccccg cttgtccatt tgctgcaata actgctagtt tcatttttta
     2641 tacctctctt attgtaatta ttttagttac aacaattgtg acactcttaa taatcaatgt
     2701 caatagtttt gcttaattat tatcaaaata tttctaccaa gaaaactaac catgattcta
     2761 gtgaaaaaaa aatcttcttt gccaacaaat ttactttctt gttttaaaca tgctataata
     2821 atcatagcaa gagatctaag ttgtctgttt ttttaaaacg aggtgattat catgcgtaga
     2881 ttctattccc atctccccta ctatctggtc atattattct tttattggcc actttatgag
     2941 ttgttcttac tagttgtttc tgaccccctt acactcaagg aactctatat aaacaatctt
     3001 ctcttcttta cacctctggt aatcttgatt gtatcgttac tctatagcta ccgtttccgt
     3061 ttctcacttt gatggttagt tggtaacgga ctgctctttt actttactat cataaccttt
     3121 ggtgagttta tactaattta cttgctaatc tatgaaacag ttgctctggt gggcatggat
     3181 tctggtatta gcatcaagca tattctacaa aaaatgaaaa acaaaaaact ttcacaaaat
     3241 ccttgaaaaa tctcacaatc atgctataat aatccataga gacaagtcac ttagtccctt
     3301 tctactagag agtgcgtggt tgctggaaac gcataggaag tctaaactga tactactctt
     3361 gagtttttta tgaaaacata aaacggtggc cacgttagag ccaatcagag gtgtccctct
     3421 cttttgaggt acataaatga aggtggaacc acgttgcgac gtcctttcga ggatgtcgca
     3481 tttttttatt aggatactaa ttatggagtt gcaagaatta gtggagcgca gttgggcaat
     3541 ccgacaagct tatcacgaac tggaagttaa gcatcatgat tccaagtgga cggtagaaga
     3601 agaccccttg gctttatcta atgatattgg aaatttccaa cgactggtga tgacaaagca
     3661 aggacgctac tatgatgaaa caccctacac actggaacaa aaactttcag aaaatatctg
     3721 gtggctatta gaactttctc aacgtttgga tatagacatt ctgacggaaa tggaaaactt
     3781 cctctctgat aaagaaaagc aattgaacgt taggacttgg aagtagtctg atgataaaaa
     3841 atcaatgctt agaaactatg aaataataaa aaaggagaac atcatgatta acattacttt
     3901 cccagatggc gctgttcgtg aattcgaatc tggcgtaaca acttttgaaa ttgcccaatc
     3961 tatcagcaat tccctagcta aaaaagcctt ggctggtaaa ttcaacggca aactcatcga
     4021 cactactcgc gctatcactg aagatggaag catcgaaatt gtgacacctg atcacgaaga
     4081 tgcccttcca atcttgcgcc actcagcagc tcacttgttc gcccaagcag ctcgtcgtct
     4141 tttcccagac attcacttgg gagttggtcc agccatcgaa gatggtttct actacgatac
     4201 tgacaacaca gctggtcaaa tctctaacga agaccttcct cgtatcgaag aagaaatgca
     4261 aaaaatcgtc aaagaaaact tcccatctat tcgtgaagaa gtgactaaag acgaggcacg
     4321 tgaaatcttc aaaaatgacc cttacaagtt ggaattgatt gaagaacact cagaagacga
     4381 aggcggtttg actatctatc gtcagggtga atatgtagac ctctgccgtg gacctcacgt
     4441 tccatcaaca ggtcgtatcc aaatcttcca ccttctccat gtagctggtg cgtactggcg
     4501 tggaaacagc gacaacgcta tgatgcaacg tatctacggt acagcttggt ttgaaaagaa
     4561 agacttgaaa aactaccttc aaatgcgtga agaagctaag gaacgtgacc accgtaaact
     4621 tggtaaagag cttgacctct ttatgatttc acaagaagtg ggacaaggtt tgccattctg
     4681 gttgccaaat ggtgcgacta tccgtcgtga attggaacgc tacatcgtaa acaaagagtt
     4741 ggcttctggc taccaacacg tctacactcc accacttgct tctgttgagc tttacaagac
     4801 ttctggccac tgggatcatt accaagaaga catgttccca accatggaca tgggtgacgg
     4861 ggaagaattt gtccttcgtc caatgaactg tccgcaccac atccaagttt tcaaacacca
     4921 tgttcactct taccgtgaat tgccaatccg tatcgctgaa atcggtatga tgcaccgtta
     4981 cgaaaaatct ggtgccctca ctggccttca acgtgtacgt gaaatgtcac tcaacgacgg
     5041 tcacctattc gttactccag aacaaatcca agaagaattc caacgtgccc ttcagttgat
     5101 tatcgatgtt tatgaagact tcaacttgac tgactaccgc ttccgcctct ctcttcgtga
     5161 ccctcaagat actcataagt actttgataa cgatgagatg tgggaaaatg cccaaaccat
     5221 gcttcgtgca gctcttgatg aaatgggcgt ggactacttt gaagccgaag gtgaagcggc
     5281 cttctacgga ccaaaattgg atatccagat taaaactgcc cttggaaaag aagaaaccct
     5341 ttctactatc caacttgatt tcttgttgcc agaacgcttc gacctcaaat acatcggagc
     5401 tgatggcgaa gatcaccgtc cagtcatgat ccaccgtggg gttatctcaa ctatggaacg
     5461 cttcacagct atcttgattg agaactacaa gggtgccttc ccaacatggc tggcaccaca
     5521 ccaagtaacc cttatcccag tatctaacga aaaacacgtg gactacgctt gggaagtagc
     5581 caagaaactc cgtgaccgcg gtgtccgtgc agacgtagat gagcgcaatg aaaaaatgca
     5641 gttcaagatc cgtgcttcac aaaccagcaa gattccttac caattaattg ttggagacaa
     5701 agaaatggaa gacgaaacag tcaacgttcg tcgctacggc caaaaagaaa cacaaactgt
     5761 ctcagttgat aattttgttc aagctatcct agctgatatc gccaacaaat cacgcgttga
     5821 gaaataagag tctagcataa aagcctccaa tctggaggct ttttctcatc tatttttact
     5881 caaggactaa gttcacttga gcaaactgaa tccgcactgt cgttcctttt ccgacctcag
     5941 actcgatacg aatctggtgc cccagttctt cagaaatttt cttagataga taaaggccaa
     6001 gtccagagga ctgctgggtc aaacggccat tgtatcctga aaagccacgt tcaaatactc
     6061 ggaggacatc actgtttttt atcccgattc ccgtatcttt gatacaaagc tcttggtcat
     6121 ccatataaat ctccagacca ccttccttgg tgtacttgag actgtttgag atgatttgct
     6181 caataaccac tagcagccac tttttatccg tcacgatttc tttatcaagg tcatgtagat
     6241 tgacatttaa gcctttttga ataaagaaaa gagcatattt acgaattatt tccttgacca
     6301 agtcctcaat ttgaacctgc tttaagacca aatcatcatg gaaactttct aaacgcaggt
     6361 actgtaaaac taggttggta taagagtcga ttttgaaaat ttcctgttct agctgctgct
     6421 tcagttggcg gtcgaccact tctgcaacta agagttgact ggctgcaatg ggggtcttta
     6481 tctgatggac ccacaaggta tagtaatcca gcaaatccgt cagttttctt tctgcttttg
     6541 acctctgctg atagagttcc atctcacgcg cttctaattt ttctgctaaa gctatttcca
     6601 aaggagactt ggcttccctc tctccataga gaagttcctg gcgatagacc tgcatttcca
     6661 ccaatatgtc ccaagtgaaa aataagatgg ttacaaagca acacaagaag aaaaagtaga
     6721 ggaagtaaat tcctagactg gcaaataaaa actgaaagag taagacaaga aatgccaaag
     6781 aaagcagata gataaaaaga cgactacggg agcgcagata ggctagaaaa aattgtttcc
     6841 aatcaagcat gcttcaatcc gtaccctatt cctttcttgg tctcgataaa tcctaccaat
     6901 ccctgctcct ccaacttttt acgcaaacga gccacattga cagagagggt attatcatca
     6961 atgaaaaagt cactgttcca aagttcccgc atcaggtcgt cacgtgctac gatgttgcct
     7021 gcatgctcaa ataacacgcg taaaatctgg aattcattct tggtcaaatt caagacttgc
     7081 ccttgataat gtaaatccat ggatttggta ttgaggataa caccagcata ttccagcaaa
     7141 ctctcatcac gcccaaactc ataggaacga cgcaacaagc cctgaacctt agctaaaaga
     7201 acctgctggt caaaaggctt ggtcacaaag tcatccgccc ccatattgat tgccatgaca
     7261 atatccatag cctggtctct cgaagaaaga aacatgatag gtaccttgga aatcttgcgg
     7321 atttcctgac accagtgata accattaaac aagggcaaac caatatccat gaggaccaga
     7381 tgaggttccg actgaacaaa tagactcaaa acttccataa agtcttctac caggaccact
     7441 tcaaatcccc attcagagag cattttccca atctgttgac gaatgacctg atcatcttct
     7501 attaataaaa tcttgtgcat gcgcttctcc ttttccatta ttataacaga tttttccatg
     7561 ctagatggtc tgaaactgaa tttgaaatag cctgttttta gccagtgcaa acaggctatg
     7621 ctactagcta atttgaggga aatttgctaa gataaataaa aagaaaggag ctcttatggc
     7681 caatattttt gactatctga aagatgtcgc atatgattct tattacgacc ttcccttgaa
     7741 tgagttagac attctaacct taatagaaat cacctacctc tcctttgata atctggtctc
     7801 cacacttcct caacgtcttt tagatctagc acctcaggtt ccaagagagc ccaccatgct
     7861 tactagcaaa aatcgccttc aattattaga taaattggct caacacaagc gcttcaaaaa
     7921 ttgcaaactc tcccatttta tcaacgacat cgaccctgaa ctgcaaaagc aatttgcggc
     7981 tatgacttat cgtgtcagcc tcgataccta tctgattgtc tttcgtggga cagatgacag
     8041 tatcattggc tggaaggaag atttccacct gacctatatg aaggaaattc ctgctcaaaa
     8101 gcacgccctt cgctatttaa agaacttttt tgcccatcat cctaagcaaa aggttattct
     8161 agctgggcat tccaagggag gaaatctcgc tatctatgct gctagccaaa ttgagcaaag
     8221 tttgcaaaat cagatcacag cagtttatac atttgatgca cctggtctcc atcaagaatt
     8281 gacacagact gcgggttatc aaaggataat ggatagaagc aagatattca ttccacaagg
     8341 ttccattatc ggtatgatgc tggaaattcc tgctcaccaa atcatcgttc agagtactgc
     8401 cctgggtggc atcgcccagc acgatacctt tagttggcag attgaggaca agcacttcgt
     8461 ccaactggat aagaccaaca gtgatagcca gcaagtagac acaaccttta aagaatgggt
     8521 ggccacagtc cctgacgaag aacttcagct ctacttcgac ctcttctttg gcactattct
     8581 tgaggctggt attagctcta tcaatgactt ggcttcctta aaggcgcttg aatacattca
     8641 tcatctcttt gtccaagctc aatccctcac tccagaagaa agagaaacct tgggtcgcct
     8701 tacccagtta ttgattgata ctcgttacca ggcatggaaa aatagataat actcttgaaa
     8761 attaaatgta tacaaaacaa aagacctaga atacatactt tcatgtgcat tctaagtctt
     8821 tttaaataga atctaatagt caataaaaat caaagagcat tgagagataa tggggcttgg
     8881 aacgtccctc tcgcttcaac aaaatgaccc cattatagat taaaaagatg ccacttagaa
     8941 aaagcaaaaa aggaagtaag acaaaggcaa atacataaaa agctaactga acattctcgt
     9001 atccattttt ataaaaaagg taggatagat aaaaataact tgaaatgagg gataataaaa
     9061 ataatactgg attccacaaa cttctattat ccttccaaaa tgacactata aaggctaata
     9121 caattcctat aacgagatac atttcttact cctttaatag ctacatttta tcataattat
     9181 ccaaagaaaa aagagggcat ttatccctct taatccttca tctgactctc tgcatcggcc
     9241 acgacttttt ctagactggt ttgaccaagt tctgcctcca tagtcaactg aattctctcc
     9301 aatttttgat ccaaaacatc atgaatatga gctcctacag ggcaatttgg attcggattg
     9361 tcatggaaac tgaagagttg acctgtctta ccaagacatt cgaccgcctg ataaacatct
     9421 aaaagactaa tatccttaag gtccttgaca atatctgttc cgcccgttcc acgcgctact
     9481 gaaatcagct ctgccttctt caactgggac aagatctttc tgataatgac aggattgacc
     9541 ccgacactag cagccagaaa atcactggtc accttgcttt tcttcccctc gagggcaatg
     9601 attatcagca tatgagtcgc aatggtaaat ctacttggaa tttgcatcct cttctccttt
     9661 ttacgaggct accctgcctc tactcttctt tttctattat tatacccttt ttagttgtaa
     9721 tgtcaatcgt taccactttt caaccagtcg tctaactccc gatcgcaacc ctctttctga
     9781 gccaattctc tcaaaaattc ctgatgatga gtatggtgga tcccattgac cagactttca
     9841 tagtaaacct caaaataggg aagtctcagg tctttagcca gctgcaattc agctgccaca
     9901 tcgtaatcta cccgtcggaa gtccatatct accaggccct tgtcatcaaa ctccaaaatc
     9961 atatactggg cccgcaagtc cttccgtagc tgagcgtcca aaaagaaagg ttggccaatc
    10021 gaacccggat tgacaatcaa ttgcccacca gtcccgtaac gaagcaactg ctggtgaatg
    10081 tgtccataaa cagcaatatc acagggagga tgagtcacca agcggtcaaa ttcctcttgt
    10141 ttgccagtat gaatcaactc tcgcccccag ttcttatcag gcagatgatg gctaattccc
    10201 actgtcaaat ccccaaactg acgatgaatt tgaagaggtt gattgtggag cacttcaatt
    10261 tcttctaggg aaatttcctc taaaacatac tggcactggc gcaagagata gcgttgacta
    10321 gggcgagtac tatccaattc cttacggaca ccatgccaaa gactgtcttc ccagtttccc
    10381 aaaactctag ccgtaatcgg tagttgatcc aacaagtcca aaatccttct acgccctgtc
    10441 cctggcatga gaatatctcc caaaagccag tattcatcca ctcctagctg ccgagcatct
    10501 gccaaaacag cctccaaggc ggtggtattt ccatgaatat ctgaaagaag agctattttc
    10561 gtcatatcca tctcctcgtt ttttctcttg caataagtat aacataaaaa gtcacagcta
    10621 gagaaatcta gctttttttg atatactaga taaagatatt agacaagagg aaacgaatga
    10681 ccccaaacaa agaagactat ctaaaatgta tttatgaaat tggcatagac ctgcataaga
    10741 ttaccaacaa ggaaattgcg gctcgcatgc aagtctctcc ccctgccgta actgaaatga
    10801 tcaaacgaat gaaaagtgaa aatctcatcc taaaggacaa ggaatgtggc tatctactga
    10861 ctgacctcgg tctcaaactg gtctctgagc tctatcgtaa gcaccgcttg attgaagttt
    10921 ttctagttca tcatttagac tatacaagtg accagattca cgaggaagct gaggtcttgg
    10981 aacacactgt ctctgacctg ttcgtggaaa gactagataa actgctaggt ttccctaaaa
    11041 cctgccccca cggaggaact attcctgcca agggagaact cctcgttgaa atcaataacc
    11101 tcccactagc tgatatcaag gaagctggcg cctaccgcct gactcggatg cacgatagtt
    11161 ttgacattct ccattatctg gacaagcact cacttcacat cggtgaccag ctccaagtta
    11221 agcagtttga tggcttcagc aataccttca ctatcctcag taacgacgag gatttacaag
    11281 tgaatatgga cattgcaaaa caactctatg tcgagaaaat caactaattt ctcaagtccc
    11341 ctaccaaccc tgaaagtttt attttcaggg ttttatttct atcattttat tttactttca
    11401 tttggtggta gaatatttga aaaaaaatgg gagaatattt gaaaaaaaga aaggtagttt
    11461 aacctatgaa aaaatcacta aaaatttttg ctacatctaa atagtttgac ctcttcgggg
    11521 ttgctttggt cgttggattg cgattgcatc tggttacctc aactcacgcc tcgataaatt
    11581 tgtagactgg ggaccatgga cagctcttgt accctttgga ttgatttccg taaccaacgt
    11641 tgggatttcc atgttgtcca ctcgtttcac ggggaaatta agcaaatggg aaaattactt
    11701 tggtattgtt aataccattt tgtccggtgc tattgattat atccttggaa ataaggcggc
    11761 catcattacc tatcccgtca ccttcctcat ttataccttt gctataaaga aatggaaagc
    11821 ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag gtaaaattgg cggccatcat
    11881 catttccatc atcgccttcc tctttgcctt tgtgaccaac tatatcggat atgggggcaa
    11941 gatgaatctc cttgcctacg taacaactat tgcctttgca ctgtccctca ttgccaatgc
    12001 tttgaatgca ttaaaactaa caactcagtg gggcttttgg ttgatttaca atttcgttca
    12061 gctgacaaaa gctggtattc aaggaaactt cgccaatatc ggtaaataca tcttttatat
    12121 cctcaatgca atcggagctt tatttgtctg gaatgatgaa gaagttagat aatagaagga
    12181 aactcaaata aagaatacta gccaacttaa gagaatctcc cctctgctat ttttatcatt
    12241 ttagttgtaa ctcccattct tttagagtag aatgaagtta gataaaagag ggaggtcttg
    12301 tcatgaaaaa actctataga atccatttta tagcaattgc agttattgat ttgctactat
    12361 ttgcattttt tatcactaga ctcgagacat cttttgagtg gctcttgctc tctggtttaa
    12421 ttttcttcct tgctcaagga ctcctgctat ttctcttggt cgtccgactc aaacatcaat
    12481 tcgctgagat ttatcctcaa atcaataaaa agattcgctt ctactattta ggggttctca
    12541 ccattgattt tctatttttt gttctcttag ccttcattag ttctcagcgt ttttcatctc
    12601 ttatgccaat catcactgct tgccattcta ctttttatta tatgacagct gactacctaa
    12661 gagaaaacta tccagacttt tacgacaaac acatctcttt atgggagtgt ctctaaggaa
    12721 aaggaggttt tagcatgaaa aaaatcatct tcatcaaaac cattcaactc cttgtcattg
    12781 atggaatcat gctggtattt ttgacattta aaagggggcc ttacttggga ctggattttg
    12841 atttatagtg gttggctcat tttctttcat cccgtgctat tgacctatct ttcaaaccaa
    12901 ctttgtgacc actttagtca actctattcc cagattagac cgagattctg gcgttttgct
    12961 ttacaaattc tcctatggga tagcctgatg attctctcct tggtgtcttt aagtgatatt
    13021 ccacttttcc ttcagggaac tctcctcatc ctaggacatc tcatcccttc ctatcgcatc
    13081 tgccaaagcc tgaaaagaga cttcccccaa gcatatcaag aaccgatttc tttttggagt
    13141 attttatgat cgatgagaaa gaccaagccg actgggcttg gtctttctta tctcttttta
    13201 gtatctagga taatggtaac aggtccgtta ttaaccagct caacctgcat atctgctcca
    13261 aagatacctg tctgaacggg cacttcttgc gctaattttt gattgaaagc atcatagaag
    13321 tctgatgcca tatcaggttt agctgcccct gtaaaggctg gacgattgcc tttcttagta
    13381 tccgcaaaga gggtaaactg agaaatagag aggatttctc cttcaatatc tttgacagac
    13441 aggttcatct tgccttctgc gtctgaaaaa atccgcatat tgaccagttt tctcacagca
    13501 tagtccaaat cttcctcttg gtcctctggt ccaacaccaa ccagcaataa aagtccctga
    13561 ttgatttttc cctgaatctg gccttctata ctcacttggg cttttttaac ccgttggata
    13621 atgattttca taatagcctt tctagtaaga gctaggacaa ctagccgttg gtccgtttga
    13681 cagagtaaac ttctggcaca ctcttaattt tatcgacaac cgtggtcagt gtagagaggt
    13741 tggcaatacc gaaggacaca tggatattag caaacttcat atccttggtt ggttgggcat
    13801 tgaccgttga aatattcttg gttgtatttg aaagaacttg cagtacatcg ttcaacagtc
    13861 ctgtacggtt gagaccgtag atatcgatat gggccatata ctccttattt gagctagagt
    13921 actggtcttc ccattccaca tcaaggagac gttgctcgta gttttcttgg gcacgcaggt
    13981 tcatacagtc cacacggtga atagccacac cacgaccctt ggtaatgtag ccaacaatat
    14041 cgtcaccagg cacggggtta caacacttag caatccgcac taggagacca gaagcacctt
    14101 caataaccac tcccccctca tgcttgacct tgagagtttc tttattttca accttgacct
    14161 cgccaccttt gacaagctcc tctgcctcag ccttggcctt ggcacgctct tcctcacggc
    14221 gttccttttc agtcagacgg ttaaagacgg taatcgcacc gatttcccca aaaccaatgg
    14281 ccgcaaagag ggagtcttct gtcttgtaac tggtcttttg cagaacttga tccatgtggc
    14341 gcttgtccat aaatttattt gccacatagc cattttcttg gaactgagcc atcagcatct
    14401 cacgaccctt gttgacagac aattccttat cttggttttt aaagaactgg cgaatcttat
    14461 tgcgcgcctt gctagtcttg accatattga gccagtcacg gctaggtcca aaggagttcg
    14521 ggttggcgat aatttcaacc tgatcccctg tctttaactt ggttgtcagt ggaaccatgc
    14581 ggccattgac cttggcacca gttgcttttt caccgacctt ggtatggatt tcgtaggcaa
    14641 aatcaatcgg tcctgaatct ttgggaaggg aacggacagc tccatctggg gtaaaaacgt
    14701 aaatctcctc agccaaatag ttttccttaa cagagtccac aaattcctta gcatcatcag
    14761 cctggtcttg gagctccatc atctccttga tccagttcat tccaatagct gattccttgc
    14821 tgttaacttg cccctttata cctttcttat aagcccagtg agccgcaacc ccgtactcag
    14881 ccacctcgtg catttccttg gttcgaatct ggaattcaat cggccctttt ggtccataaa
    14941 cagtcgtatg gatagactga taaccattgg ccttgcggtt ggcaatatag tctttgaagc
    15001 gacctggcat cggtttccaa aattcatgca cgtaaccaag catggcataa acatcacttt
    15061 gggtatctaa aatacaacga atagcaatca gatcatagat ttcctcaaac cgttttctct
    15121 tgtcctgcat tttgcggaaa attgagtaaa tatgcttggg acgaccataa atcttccctt
    15181 tcaagtgacg ttctgtcgta tactcctcta attttgtgac tacctcatcc accaaggcct
    15241 cacgctccct gcgcttttcc ttcatcatat gggtaatctt gtaaaactcc gttggattga
    15301 gataacggaa agacaagtct tctaattccc atttgacact ggaaatcccc aaacgatggg
    15361 caagtggggc atagatttcc atggtttctt tggaaatacg ctcctgcttg tcttttcgaa
    15421 gatgtttcag ggtccgcata ttgtgcaagc ggtcagacag tttgaccaaa ataacgcgga
    15481 tgtcctcaga catggccatg agcatcttgc gatgattttc cgctaattgc tcctcgatcg
    15541 atttgtactc gaccttgcca agcttggtaa ctccgtcaac aatcacccgc acatcaggac
    15601 caaactctct ttccaaatcg tccaaggtcg catctgtatc ttccaccaca tcatgcaaga
    15661 atccacaagc tactgttaca gcatccagct ttagcttagc taaaatacct gccacttgga
    15721 tagggtgaat gatataaggc tcgcctgatt tgcgatattg accactgtgg cattcaacag
    15781 catagaccaa ggccttatgg acaaaatgaa catcctcttc cgttaaatat tctttggtta
    15841 aagcgacaac ttcttcgcct gttaaattca cttctttcgg catctctact ctccaattct
    15901 tcctaccatt ttatcacttt tttaagaata tgaaaactag attggaacag aataagaaaa
    15961 aaataattca aaattgcttg ataattctga attattggcc cataatatac tacgaagtta
    16021 gattttaaac ttaggtgata gaaggagaga tagaagaacg gaaaccatat tgtaacccaa
    16081 agactttctg atttccccaa ttccattgaa gatacgaaag ataaacggtg gaactcgtat
    16141 cacatacact ggtaccttga ctggattttg gaattaatac tcaatgaaaa tcaaagagca
    16201 aactaggaaa ctagccacag gttgctcaaa gcactgcttt gaggttgtag ataagactga
    16261 caaagtcagt cacatatata cggcaaggcg acgttgacgc ggtttgaaga gatttttgaa
    16321 gagtataaaa atcctcaaga tactttcttc tatcctttag tttataagga gaatacctat
    16381 gaaaaaactg ctatttctat ctttgctctc ctaatgttag gagtttgctg cctgttccta
    16441 ttcagccagc aaagctataa aaaactcgtt caatactatg ctaacgacca gaacctgccc
    16501 agtaggataa cttatagtga atatagcgac aaatgagaag ccaactacgg tagcactcta
    16561 aacatcacgt ctatcaaaca agctaatgac ggagtttatg caacctatga agggcaattg
    16621 acacctttcc aatattgata aattgataac cagcctgtct tcatctagtc atgctggttt
    16681 ttaagttcat tttaaatcct tacctattct ccctaactgt gctatactta atttatactc
    16741 aatgaaaatc aaagagcaaa ctagaaagct agccgcaggc tgttcaaagc actgctttga
    16801 ggttgcagat aaagttgacg cggtttgaag agattttcga agagtattag tacattcttt
    16861 gagattggag ttagtatgaa aatccataaa accgtgaatc ctgttgccta tgaaaatacc
    16921 tattatctag aaggcgaaaa gcacctcatc gtcgtcgatc ctggtagtca ttgggaagcc
    16981 attcgtcaga caatcgagaa gatcaacaaa ccgatctgtg ctattctctt gacccacgcc
    17041 cattatgacc atatcatgag tctggacttg gttcgcgaga cgtttggcaa tcctcctgtc
    17101 tatatcgcag agagcgaagc cagctggctc tacactcctg tcgataatct ctccggtctc
    17161 cctcgccacg atgatatggc agatgtggtc acaaaacctg cagaacacac ctttgtcttt
    17221 cacgaagaat accaactaga ggaatttcgt tttaaggttc taccgacccc agggcactct
    17281 atcggtggtg tttccctagt ctttcctgat gctcacctag tcttgacggg agatgctcta
    17341 ttccgcgaaa ctatcggacg gaccgacctt ccgactggta gcatggagca actccttcat
    17401 agtatccaga cccaactctt caccctacca aactacgatg tctatccagg acatggtcca
    17461 gctactacta tcgctcacga aaaggccttc aatccctttt tctagcaaga tgatgacaat
    17521 cgaaatttaa gtaaactatc cagcaaatct ttctattaca aaaggcatcc tatcaaggtt
    17581 ttcacacgtg attggatgcc ttttttctga tgactagatt ttttgtatta ccaaataatc
    17641 acgcgctcct ctggtgaacg ccacattccg tctccttctt tgacatcata ggttgtaaag
    17701 aaatcgtcga agtttggtac ttgcacattg acacggagtt tggctggtgc gtgcacatcg
    17761 acgctagcca aaagtttcat aaattctggt cgacctttca tgcgccagat gcgaccgaag
    17821 ttgtagaaga actcttctgc tgagaagtct gcttctctct tagctgcttc aagcgctgct
    17881 gcgattcctc ccaagtcagc cacgttttct gatacagtca atttaccgtt aatggttgct
    17941 ccataagaat cctgtccatc aaattggtca atgacttttt gtgttttctc cttgaaggca
    18001 gcatagtcgc tctctgtcca ccaatccttg aggctaccat tttcgtcaaa ggaagccccg
    18061 ttagtatcaa aggcgtggga aatttcatgg gcaatcactg ccccaatacc accgtagtta
    18121 gcagaagatg actgatgcaa gtcatagaaa ggcgcctgta aaatggccgc tggaaagaca
    18181 atcaggttct tctgaggatt gtagtaggca ttgaccatat gagcaggcat gccccattcc
    18241 ttatagtcta caggctggtt ccacttactc caactgtgct tgatttccac acgcgcaaag
    18301 gctagagcat tctcaaaaag actggcagtt tcattcacta ccttatcctt gtaacgtgca
    18361 ggcaattctt ctggatagcc aatataaggt ttgatcacat tgagcttcac gatagcctgt
    18421 ttacaggttt ctggagtgag ccagtcattc ttaagcagac gctccttata gacatcaatc
    18481 atggttgcca cttttttctc cacatccgcc ttggcttctg gagagaactt ctcacgggcg
    18541 taccaaagcc ccagggcttg cttgaaaggt tcctgtgcta gatgataagc tgctttgacc
    18601 ttatcttttg cctctggaac tccagaaagg gcacggctgt aggcaccaga caaaacacgg
    18661 atatcctctg ttaaatagct ggttgaaaga ttgacaacac tcaaaatcaa ggttgcttta
    18721 aggagagacc aggcttcctc actgtaaaat tgctctgctg cttgccagaa acgttcctcg
    18781 tctacaataa ccttgtctgg taattgccca ataactgctt tgaagaagtc atccaaaggt
    18841 agggcaggcg cgaatttctt gaaatcttcg taagaatatg gatgatagag tttagcatat
    18901 tctgaacttt cttcattaga gagcaccact gccgcaactc ggcggtccaa ttcaagtctt
    18961 ttttctagca agtcttcaat ttcttcatca gagaaatcat aagccttgag gagatttgcg
    19021 ctgctttctt tccaaagagt caagagctct tcgcgctgag gatgttcttc tgcatagtag
    19081 gtcgtatctg gcaagattgt gcttggagcg ctagcccata gaacattgat tctagcatcc
    19141 ataaagtctg gcgatacacc aaaaggaagg aagtttggtt ttcctgcaag ctcaaactct
    19201 gctagtttag ctgtaaaatc cgcaaaagtt tccaattctt ggaattcttt aaggagtggt
    19261 aagacaggtg tgataccgtc agcttctctc ttgtcaaaat cacgaactag gcggtggtat
    19321 ttgacaaagt tttccaagat agcatcctca ggcacttctt cacctgctaa ccacttgtct
    19381 gttgtcgcca gcatcaggtc ttcaatttcc tggtctaaat caacaaaacc tcctgtttga
    19441 gacttatctg ctgggatttc agctgtctgt tgccattttc cattgatagc atcataaaaa
    19501 tcatcttgat aacgtgtcat cttgttctcg ctttcatttg tatttgcatt tatcttaaca
    19561 aaaatcacta gggaatgcaa ttaataacca tttaactatt attttttagc tat
//
LOCUS       DAVLFB010000030        19606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLFB010000030 DAVLFB010000000
VERSION     DAVLFB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.030"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            385..567
                     /locus_tag="VXD58_000988"
                     /pseudo
     CDS             385..567
                     /locus_tag="VXD58_000988"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1179..1463
                     /locus_tag="VXD58_000989"
     CDS             1179..1463
                     /locus_tag="VXD58_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675709.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1456..1694
                     /locus_tag="VXD58_000990"
                     /pseudo
     CDS             1456..1694
                     /locus_tag="VXD58_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1790..2008
                     /locus_tag="VXD58_000991"
     CDS             1790..2008
                     /locus_tag="VXD58_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675710.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2375..4699)
                     /gene="pflB"
                     /locus_tag="VXD58_000992"
     CDS             complement(2375..4699)
                     /gene="pflB"
                     /locus_tag="VXD58_000992"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV0675711.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5093..6154
                     /gene="dinB"
                     /locus_tag="VXD58_000993"
     CDS             5093..6154
                     /gene="dinB"
                     /locus_tag="VXD58_000993"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV0675712.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(6301..7146)
                     /locus_tag="VXD58_000994"
     CDS             complement(6301..7146)
                     /locus_tag="VXD58_000994"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV0675713.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            7401..8372
                     /locus_tag="VXD58_000995"
     CDS             7401..8372
                     /locus_tag="VXD58_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV0675714.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIETTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKVCARF
                     SPAFKAYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
     gene            complement(8571..10460)
                     /locus_tag="VXD58_000996"
     CDS             complement(8571..10460)
                     /locus_tag="VXD58_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV0675715.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNFFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            10598..12163
                     /locus_tag="VXD58_000997"
     CDS             10598..12163
                     /locus_tag="VXD58_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0675716.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            12163..12903
                     /locus_tag="VXD58_000998"
     CDS             12163..12903
                     /locus_tag="VXD58_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0675717.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(13012..13227)
                     /locus_tag="VXD58_000999"
     CDS             complement(13012..13227)
                     /locus_tag="VXD58_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675718.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(14098..15348)
                     /gene="ilvA"
                     /locus_tag="VXD58_001000"
     CDS             complement(14098..15348)
                     /gene="ilvA"
                     /locus_tag="VXD58_001000"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV0675719.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(15398..15754)
                     /locus_tag="VXD58_001001"
     CDS             complement(15398..15754)
                     /locus_tag="VXD58_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0675720.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(15992..16228)
                     /locus_tag="VXD58_001002"
     CDS             complement(15992..16228)
                     /locus_tag="VXD58_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675721.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(16335..17357)
                     /gene="ilvC"
                     /locus_tag="VXD58_001003"
     CDS             complement(16335..17357)
                     /gene="ilvC"
                     /locus_tag="VXD58_001003"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV0675722.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(17423..17899)
                     /gene="ilvN"
                     /locus_tag="VXD58_001004"
     CDS             complement(17423..17899)
                     /gene="ilvN"
                     /locus_tag="VXD58_001004"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV0675723.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(17892..19592)
                     /locus_tag="VXD58_001005"
     CDS             complement(17892..19592)
                     /locus_tag="VXD58_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV0675724.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         6118 a         4168 c         3505 g         5815 t
ORIGIN      
        1 tttttgtttt tacaattctc cttagttaaa tcttgtttaa tatatgtttt acatatagta
       61 tttagcgcca catagtactg aactctctcc aaaaacggtt attcctcttt gaatagggcg
      121 ttatcacaag aaaagcatct ccacgtttca acttcatatg gctcaaaaac aatcaattga
      181 tgctaaaacc tgtacctaga tgtttcggtt cataaaacca tgaaactgta aaagtgaatg
      241 aaattgatag cgatagtcaa atcaagaggc atcataactc taaaaagtca caatatataa
      301 gttcatcctc ggaaaaatat cattctaatt gttgaaatgc ctacatgaaa agaaacgtca
      361 aatgctcatg aaacaacgaa tacaggtatc aaaactatga caaaacaagt ccctaaattt
      421 actaaagaca ctgctcaact ttacacctgt aagtggttgt tgtataataa agttacaaag
      481 atgtacgacc acactgttgt aaatcatagt gttcgcgaat atattactga tagcatttct
      541 acaaatacaa ttgaagagac tggatagact ttaaacgaaa tatgtcagtg ttttggcatt
      601 cctagccttc atatcattta aagaattcta tagacaaaat ttttcccaat acagacactc
      661 gtaacaactg cttcattttt ctaccaacat atttaggaac aggataagat acaagaggat
      721 taatccatag atcagttcta taccaatcta agacaaataa gctaaaaaaa cgattgataa
      781 taagcaaata gattccaaat tttctctatc tgctcatttt aataaacaat actagtgtaa
      841 ctatccttcc agtcagaaac ttgtcaaatc acaccgaaaa ctcttctaaa atttatctcg
      901 ttaggcaatc aagcaaaaac tcgacgatag tacaaacatt atcatacaga attgactgcc
      961 taaattatat actttagtaa ggttttcgga taagaaaaaa ggttcatttt acatttctaa
     1021 acattctttt ctaagatgaa aaacagaatt tttcgattgt gatttaaagc aacaagaaga
     1081 ttttcagtat catcctatag atacgagcta attaagaaaa actacatttt tgaatataaa
     1141 ctacaataat ataaactaaa ttttatagga ggaagacaat ggattggtac gattatatga
     1201 tacaggcatc caaacaatca caattcaacg caagccattg gtttcgctat ttgcgaaaag
     1261 ttatttttga agactattct tatttaacaa accaagatgt agaaaagttg ctagactcca
     1321 aagaactaac ccgttttcaa aaaattagct tgaagtatgc ctttcaagag catactccaa
     1381 ctcataaata tgtgatttca ttaaataaac ctgctaagtt aaccaatgtt caaaaattga
     1441 tggagaaata caaacatgga taaaatgaaa ccggtcttcc aagccctaaa taagaaatta
     1501 attcaggaaa atctgacttt aacaattatg gttatgtctt agaatatcat ggtttacgta
     1561 ccacacaaga tgttgatgct ttttatgacc aaaatcagaa aatcaatgaa attattgctc
     1621 gtgtaggaaa acagtttaat cttaacatcc acgaagagtt atggctcaac aatcatgttg
     1681 caaagcaaat atgaacaaac aacctccctt aagtttatgc gaatccttgt actcatttga
     1741 gaatctgacc gttttagtag tccctattga gtatgtgtta ggaatgaaga tgatgagtat
     1801 cagagagcag gatttgcaag atattggtgc aattattaaa tacaaaaact ttcattcacc
     1861 attcgatacc tttaaatatt taaaagacat gggatttgat actattgacc tctctgttct
     1921 attagaaggt tttagttatg cttatggtat ggattggtta gaaaaatttt ttaaagaaaa
     1981 tcaagataaa cttcgcgaat tttattaaca gtatcaaaca agatgattgt caattagcca
     2041 gtcatcttat ttttatgatg aaatgaaata aaatcggaac aaatcactta gagaattcca
     2101 gtcaatttct agcactgttt gagccgtttc agcggactat agtaaaatga aataagaata
     2161 ggacaaatcg atcaggacag tcaaatcgat ttctaagaat gttttagaag taaaggtgta
     2221 ctattctagt ttcaatctat tatatttcag attcggtcca ctattccttc ccaaaaacat
     2281 tgcatattgc gccgaaggaa ttgctcatac tatcccaagc acaaaaccct cagtcgtttg
     2341 accgagggtt ccttttcatt attcaaaaac ttgattagct caatgcatcc aaggcatcat
     2401 ccattgaaag aacttcgtgg aagacacgtt gtgtcaattc agttttttgt tctggagtga
     2461 ggtatttagt atttacacag tatccagaga tacgtacgat aacgtcttca cctgacataa
     2521 ttttttcgta aacatcgttc aagtccataa cgttcaagtt aacgtgttgt ccaccgtttt
     2581 cgaagtaacc atcaaggatt gttaccaagt tatcaacttg ttcatcacga gtcttaccaa
     2641 gagcgcgagg tgatacttgt gtagtcaatg agataccgtc agctgcataa ctaaagtcaa
     2701 ggctagaaag tgagttcaag ttttgcaacc aaccaccttt agctttgtta gatgggttag
     2761 cacctggtga gaagaattca agtttagaca agttcacaga accatcttcg ttgaggtata
     2821 cacctttgtg aactggtgag ttaccagttt gtttagagta agcaacgtta gatgtgattg
     2881 tcaaaagtga tactgtagct tctgcgtctt tgtatagttt gtggctacgt agacgagttg
     2941 tgtaagcttc gatcaaccat tctgccaatt cgtttgaacg tgggtcatct tcaccccagc
     3001 gtgggtagtc accgattgtt tcgtaatcgt agatgtagcc atcttcgtca cggattggtt
     3061 taactgtagc gtatttgata gctgacaatg tatcaacagt gttagcaaat ccacagatac
     3121 cgaatcccat gttggcacgt tgtttagttg gcaagaaggc catttgaaca gcttcgtagt
     3181 tgtacctatc agtcatgtag tggatgatgt tcaaggcatc tacgtaagtg tcagtcaacc
     3241 agtcaagaga tttttcaaag ttcgctttaa ctgattcgaa ttcaagaact tcgtcacgga
     3301 ttggttcgat atcaaatact ttgtagtctt tgtgaacatc gtcgtaacca ccattcaaac
     3361 cagtaagaag ggctttaaga acgtttacac gagcaccgaa gtactggatg ttgtggcgtt
     3421 gttcttcatt ttctggatca agtggagaca cacagcatga gatacagctc atttcaccat
     3481 atccgtcttt agccattgtt gttacacctt cgtattggat agaagagtgt ttgtggctca
     3541 tgtgcataca gtagcgacgg aagttgtatg gcaatttgtc agtccaaaga actgtcaagt
     3601 ttggttctgg tgagttaccg atgttgtcaa gagtgttcaa gaaacggtag tccatcttag
     3661 taacacggtg acgaccgtcg ttacccatac cagccataga agttgtgata aaggttgggt
     3721 cacctgagta caattggtca taagcttttg tacgagcaaa tttaactgta cgaagtttca
     3781 taacgaaatc atcaacgaat tcttggattt ctgattcagt aaatgtacca cgagcaaggt
     3841 cacgttctgc aaagatgtcc aatacgattg gtacacgacc tagagatgta gcagcaccgt
     3901 taatcacacg gcagacagcc atgaaagcaa tgttaaccca ttggattgct tctttcacgt
     3961 tcatcgctgg tttgcgaaca tcaaccccgt aaaggtcacc caggcgaaca acttgttgca
     4021 atgcttggta ttgaaggttt acttcttcac gaagacggat tgtttcttca tcgatttctt
     4081 tgattgcatt ccagtcattt actttttctt gcatcaagta gtctgcaccg taaagagcaa
     4141 gacgtgcgta aacaccgatg atacgtccgc gtgagtatgc atctggaaga ccagttacag
     4201 tgtgtgcgtg acgagcgcga cgaatatttg aagtgtaggc acggaaaata ccgtcgttaa
     4261 ctgttgttac atatttagtg aagatttcgt gaacagctgg gtctggttcg tatccatttt
     4321 cttttaaagt agtttcagcc atacggatac caccttttgg catgaagttc aatttgaaga
     4381 gttcatcgtt ttggataccg aagataactt cattttcttt gtcgataaat ccagcaggga
     4441 tatcagcgat agatgttgga cgagtgtcca ttgggaaacg agtttcttcg tagtgtgctt
     4501 tagtttcttc tacaattttc ttgatgtgaa gtgaacgctc tgttggtcct gcaaggaagc
     4561 tttcgtctcc atcataaggt gtgtagttag cttgtacaaa gcgtgataca cttgcttttt
     4621 ctttccaatc tacgcctttg aagccttccc aagctttgtc aaaaatatct tgtgcttcaa
     4681 caactgtctt aacaaccata ttaatgtcct cttttttcta gtaacagtca tctgttacat
     4741 tcatgagaca agtataccat atagtaaccg attttaacaa gtgaaaaatc cctattttta
     4801 cactttcttt tctaaaacag tctatatttt atttcaaact gtattatatt tttgaaaaaa
     4861 taaagtcctt ttttcttttt ttcagaaaaa agggtataat aaaagaaaat aagcagtaac
     4921 actcaatgga aatcgaaaag caaactagga agctagccgc aggctgctca aagcactgct
     4981 ttgaggttgt aaatagaact gacgaagtca gctcaaaata ctgttttgag gttgcagatg
     5041 gaagctgacg tggtttgaag agattttcga aaagtataaa aaggtgctag gcatgttgat
     5101 ttttcctttg ctaaatgatt tgtcaagaaa aatcatccat attgacatgg atgccttttt
     5161 tgctgcggtg gaaatcaagg ataatcctaa actcagagga aaacctgtca ttattggaag
     5221 cgaccctcgg caaacaggtg gacggggagt cgtttctacc tgtagctatg aggctcgagc
     5281 ttttggtgtc cattctgcca tgagttccaa ggaagcttat gaacgttgtc cccaggccgt
     5341 ctttatctca ggaaattatg agaaatacaa agctgtggga ctccagattc gagctatttt
     5401 taagcgctat acagatttga ttgaacccat gagcattgac gaagcctatt tggatgtgac
     5461 agaaaataaa ctcggtatca agtcagcggt caaaattgct cgcctcattc aaaaagatat
     5521 ctggcaagaa ctccatctaa ctgcttctgc cggtatttcc tataacaagt tcttagctaa
     5581 aatggcgagt gattatcaaa aaccacatgg tttgacagtg attctacctg aacaggctga
     5641 ggattttctc aaacaaatgg atatttccaa atttcatgga gtaggaaaaa agacagtaga
     5701 acgtcttcat caaatgggcg tttttactgg tgctgattta cttgaagttc ctgaggtaac
     5761 cctaatagac cgttttggta gactaggcta tgatctgtat cgaaaggctc gtggcattca
     5821 caactctcca gtcaaatcca atcgcatccg taaatcaatc ggcaaggaga aaacctacgg
     5881 gaagattctc cttgctgagg aagatatcaa aaaagagctg actcttctat cagaaaaagt
     5941 cgctctcaat ctacatcaac aagaaaaagc tggaaaaatt gtcattttga aaatccgcta
     6001 cgaggacttt tcaactctta ccaaacgaaa aagtcttgct caaaaaacac aagatgctag
     6061 tcagataagc caaatagccc tgcaactcta tgaagaatta agcaagaaag aaagaggtgt
     6121 ccgcctattg gggattacca tgactggatt ttaaagctca atgaaaatca aagagcaaac
     6181 taggaggcta gccgcaggct gctcaaaaca ctgttttgag gttgcagata aagctgacgc
     6241 ggtttgaaga gattttcgaa gagtataaaa ccttgaagag ttgccccttc aaggtttttc
     6301 ttatacaaat aaacggacaa aactgtaaag tagcaaaaca ctaccaagca cgatacggta
     6361 tttaccaaaa agggtgaagt cgtgtttttt cacatagctg gtcaagaagc gaatagccac
     6421 catgctgacc gcaaaagcta ctcccatcgc gaccaagagc aaaaacaatt gcccaaagct
     6481 caagagttct ccggctttca caaatttgaa aatctttaag gcactagctc caaacataac
     6541 aggaatccca agatagaagg taaattctgt cacaactgaa cgactggttc catttaacaa
     6601 accaccgaca atcgttgcac ctgaacggct agtccctggt aaaagagcaa gaacttggaa
     6661 gagtccgata tagaaagcgg tcgtataagg aagcttgtcc aactctgtta cacttggctc
     6721 gatagcacgc gctttattgc gcttttccaa atagatgaag gcaaccccgt agataatcaa
     6781 catgagagca actgaaacca tgttatggaa gtgggtatca aaccaatcat caaatttaaa
     6841 gacaccaagt aaaggtaaag tagcaatcaa gaccttcaac catagtctcc aagtcttacg
     6901 aacttcctgt ttgtccttag tcggtttaaa aggattgagc ttgttaaaat aaatcaccat
     6961 aactgctaaa atagcaccaa gctgaatcac gacattaaac atggacataa aggcttcatt
     7021 ttgattttgg tattggatga attcctctgc taaaatcaag tgacctgtac tggaaatcgg
     7081 caaccattcc gtaattcctt caacaatccc gaagaagata gattttaaaa tttcaataag
     7141 atacatagat tactccctat attataatta gcgcagttag ctactgccaa agcccaagtg
     7201 gtatacttgg aataagccac tgtggattag ttcatttcct ccaatcgctt gacgatttcc
     7261 gaaaggatct aaccacagct tttgtttcaa ttggtaatga gtttgataac gcatagacgt
     7321 tttatctcgt ctaaggtaac aaggcaaaaa gttcgtggat aaccaagtag acttgaaata
     7381 ctatatctgg aggtactccc atgctttatg tgggcattga tatcgctaaa aataaacacg
     7441 atgttacagc cttgaatgtt ccaggaaaaa ctgttcttaa accactcact ttttcaaata
     7501 ataaagctgg ttttgaactc ttagatctgt ctcttcgaca gctcaaccaa gactgtctca
     7561 tcgctcttaa acttctttct gatcccaatc gtgaacaatt tcaacacgat aatcggcaag
     7621 tagacctaaa aatactggct agacatattc atcgtctcaa gaaaaaacag tctgattgga
     7681 aagtacaata cactcgttgt cttgatatca tctttcctga gttggataaa atcgttggaa
     7741 agcattcaga atatacctac caactcttga cgcgctaccc taatcctcag aaaaggattg
     7801 aggcaggatt tgataagctg atagaaatta agcgattgac cgcttctaaa attcaggata
     7861 tcctctcagt cgcacctcgt tctatcgaaa caacatctcc tgctcgtgaa ttcgaaatca
     7921 tcgaaatcat caaacattac aagaggctca ttgacaaggc ggaaacatgt gtcaatgact
     7981 tgatggctga gttcaactca gtcatcacga cggttactgg gattgggggt cgtttagggg
     8041 cggtcatttt agccgagatt cgaaatattc atgcctttga taatcctgct caattacaag
     8101 ctttcgctgg actggattct tctatttatc agtcaggtca gattgattta gctggaagaa
     8161 tgatcaaacg gggttcccct catctgcggt gggcactcat acaagctgcc aaagtatgcg
     8221 ctcgcttttc acctgctttt aaggcctatc ttaagactaa gttagaacaa ggaaaacatt
     8281 acaatgtagc catcatccac cttgcaaaaa aacttatccg aaccctgttt tatatcctaa
     8341 aaaagagctg ccatttgacg aacaaaaagt gatgtaattt ctgaaattaa cacaggatta
     8401 cctttgaaaa aatggacttt ttcaagggct ttttgccgtg ctctaaaatc atcgccaaat
     8461 catcaaattg aaataagaaa tagtttagat aaaactattg acttttctta tagtttgtct
     8521 ttcttctatc ttccattata tcatactttt tcagtctatg gtagctctct ttaaaaggct
     8581 ccgatactgc cgccacctcc gcctccagag aagccaccgc cagaacttcc acttccagaa
     8641 gatacggagt aggtacttgc tgtatttgcg acactagcat aatggctcat ttgcgcgctt
     8701 gaatgataaa acatactgtg ccagccataa gctacataga gattgatatc tggattttca
     8761 acttgaatct gatgaacctt catcaaatga ctaaccttgt ccgcatagcc aaataaggtt
     8821 gcatagacca agagacgatt ccaaacaaca atactttcca attcagcctt atccagtcgt
     8881 gcaatctcac gtaacatatt ttcaaaactg gtccagagat agtagacctc agctcccgct
     8941 tcatttagaa caccatcacg attatctagt cgaagcttcc aataatagaa aacagacaaa
     9001 actaacccta gaaaaccaag tattggcaaa gggaggtaaa gatagccatg aacgtctaaa
     9061 ctgtacaaga acaaaccaaa tccgataaat aggggcaaga tagtcaagac acccataccc
     9121 acttgcaaag tcttttccaa accagttaaa ggacgatagt aatctgggag cccccagaag
     9181 gaaactcgtt ttctcactcc ttcttgcatc tggttcaata cctcttcaaa agaagatttg
     9241 agttgaagcc ctcttgcttg aatccgtttt tcatcagaaa ctttggctct acgataaaga
     9301 ctatcagata ccttgtaatc cgcaaaaaaa ttggaaagag tggcttcttt tttgcctgaa
     9361 aaggctagat ttagacagtc tctctcaaat cttgacaaac catcttcttt caccaatctc
     9421 aagccaactg catctccttc tgaaataata gagacattcc cacggtctat cacatctagc
     9481 aaggtagctt gaataagttg gtcaaaagtg aatttgccag cccccttgac caagggactc
     9541 acttcctcca aggaggtcga gtagactgct tctgataaaa ccataggctc taattccatt
     9601 ggtggttcat agagacgatg atttttggca tatttgactg aaggagtggt ctttcttcta
     9661 taaataaaat agaagcagac actcaataac aaggagatag aaagtatcga agggaggacc
     9721 caagtaacga gttgtttact ctgatctttt tctctaacaa tcgagtcttc tatcttatta
     9781 aactcttcta aacgattccc tttcaagccc tgatccctag cgctagcaaa atcggtccga
     9841 gaccaatagg catgcaactc aactccacgc ttagccggaa gattgtctaa acggatagta
     9901 taatcaaggt tactcttttc aatcgttccc tctctaaaaa gtttccctgt atggaaaaag
     9961 agtttctcag cccccttgtc tccccttaca tgaaattcaa acttttcaat agactctgaa
    10021 ctatctgtca gaggttgcca atttaattca gcgatatcat cataaaggaa aagtaaattt
    10081 tttaagttcc agacgaggtc aacttcaact atgtcgccct cctgacctgg attatagact
    10141 ctcacagtat aaccatccgc ttcttctgtt acttcgctag tcacatctgc tagttctgca
    10201 ccgtttttcg cagcctgaac ctttggatga gggtcaatgt caaacccgct aggcatctta
    10261 ccagcacgtc caagtcccac gatttggccc ttaaagtcct cctcaaactg gtaaactatc
    10321 ttctgtctaa actctgccgt attatctgca tgaatataca aatccccttg ataagagttt
    10381 atcttgaaat caatggcaaa aacagagagt ggaagaaggc aaaacaagcc taacaccagt
    10441 aagaaaaaag tttttttcat caactaagcc ccctttttat ctttcttatc attatagcat
    10501 cttttcgtaa gtaaaggctt acttatgttg aaaaattacg catttttcga taaaatagga
    10561 gacagttatt ttttaataga tcagaatagg agaaatcatg agaaaaatat acctatctat
    10621 tttcacaagt ctcttgctga tgctaggact tgtcaatgtt gctcaagccg atgaatattt
    10681 acgcatcggg atggaagcag catatgctcc ctttaactgg acccaggatg atgatagcaa
    10741 cggagctgtc aaaatcgatg ggaccaatca gtatgccaac ggatacgatg ttcaaatcgc
    10801 caagaaaatc gctaaggact taggtaaaga acctttggtt gttaaaacca agtgggaagg
    10861 tctagtccct gcccttactt ctggtaagat tgacatgatt atcgcaggta tgagtccaac
    10921 tgcagaacgc aaacaagaaa ttgccttttc gagcagttac tacactagcg aaccagtttt
    10981 gcttgtcaaa aaagattctg cctacgcaag tgctaaatct ttggatgact ttaatggtgc
    11041 gaaaatcact tctcaacaag gtgtctacct ctacgacttg attgcacaaa tcccaggtgc
    11101 taaaaaagaa acagccatgg gagacttcgc tcaaatgcgc caagctcttg aggctggtgt
    11161 cattgatgct tatgtttctg aacgtccaga agcactgact gctgaagctg cgaactctaa
    11221 gttcaagatg attcaagtag aacctggttt caaaactggg gaagaagata cagctatcgc
    11281 tatcgggctt cgtaaaaatg acaatcgtat tagccaaatc aatgccagca ttgaaaccat
    11341 ttcaaaagat gaccaagttg ccttgatgga tcgtatgatc aaggaacaac ctgccgaagc
    11401 tacaacaact gaagagacta gcagtagttt ctttagccaa gtcgctaaaa ttctttctga
    11461 aaactggcaa caactcttgc gtggtgctgg tatcactctt ttaatctcta tcgtcggaac
    11521 catcataggt ctcattattg gacttgccat tggtgtcttc cgtactgctc ctctctctga
    11581 aaacaaagcc atttatggcc ttcaaaaact agtcggctgg gttctcaatg tctacattga
    11641 aattttccgt ggtacgccaa tgattgttca atcgatggtt atctactatg gaactgccca
    11701 agctttcggg atcaaccttg accgtacact ggctgctatc ttcatcgttt caatcaatac
    11761 cggtgcctac atgactgaaa tcgtccgtgg tggtatccta gcagttgaca agggacaatt
    11821 tgaagctgcg actgctcttg gtatgaccca taaccagacc atgcgtaaga ttgtcctacc
    11881 tcaggtagtc cgtaacatcc tacctgcaac tggtaatgaa tttgtcatca atatcaaaga
    11941 tacatctgta ttgaacgtta tctctgttgt cgaactttat ttctcaggaa ataccgtggc
    12001 aacacaaacc tatcaatact tccagacatt tacaatcatc gccgtgattt actttgtcct
    12061 caccttcacc gtaacacgta tcctacgatt tatcgagcgc cgaatggaca tggataccta
    12121 tactacaggt gctaaccaaa tgcaaacgga ggatttgaaa taatgacaca agcaatcctt
    12181 gaaattaaac acctcaaaaa atcctatgga caaaacgaag tgctaaaaga catttcactc
    12241 actgtccaca agggagaggt catctctatc atcggaagct ctggaagcgg aaaatcgacc
    12301 ttcctacgct ccattaacct acttgaaaca ccaactgatg gacaaatcct ttatcatgga
    12361 caaaacgtcc tcgaaaaagg ctatgacctc acgcaatacc gtgaaaagtt ggggatggtt
    12421 ttccaatcct ttaacctctt tgaaaatctc aatgttcttg aaaacacaat cgtcgctcag
    12481 acaactgtcc taaaacgcga acgcacagaa gctgaaaaga ttgccaaaga aaacctggaa
    12541 aaggtcggca tgggagaacg ctactggcaa gccaaaccaa aacaactctc aggtggtcaa
    12601 aaacaacgtg tggccatcgc tcgtgccctc tccatgaatc cggacgctat tctctttgat
    12661 gaaccaacat cagctctcga tccagaaatg gttggagaag tcctcaaaat catgcaggac
    12721 ctggctcagg aaggcttgac catgattgtc gtaacccatg aaatggaatt tgcccgtgat
    12781 gtctctcacc gtgttatctt tatggataag ggcgtgatcg ctgaagaagg taaaccagaa
    12841 gacctcttca ccaatcctaa agaagaccga acaaaagagt tccttcaacg ctatctcaaa
    12901 taaaaagaaa aggctgcatc aaccgtgcag tctttttgct gtcctcaaaa tgaaaaggca
    12961 gattctctat ctctactact tgaaggtcaa gaatccgccg ttatccaaag attaatcttc
    13021 aaatttttca taattttttt catagaaatc aattaagcct agggctgttt caaatgaaat
    13081 attttttact tttgcacgac cctgcgccag agcaatgata gacatttcac gcgcattcgt
    13141 ttctttagag atacggtagc ctgtgatttt cttatcacga acccagccaa caactgattc
    13201 tactttttca aagtttgact tagccatgtt tttctcctat tttcttcttt ataatattta
    13261 attgtatacg tttttactac ttttgtcaat aaaaaaaaca tatattcaac gaaaataatt
    13321 agttcagaat tttcttactt cctttttaaa tccgataata tataggtttt gctagcctct
    13381 acaccataaa tatttgctaa taaaactact gtattattac cctcttaagg tgcgaaatta
    13441 ttcaagttct taaaaaatgc ttctctattt ccgtaagata gcttatttct tcgatttaag
    13501 aactgaagat gatagttcta acctttagaa atccaagcct tcactagctc aaaaactcct
    13561 atctttcttc ctcaataaat catctgagag ataggtattt cttatatcct tcaaattcat
    13621 cactcctttg gatattgagg tgctagccta gaagcagaca cccgattatc taaaccatcc
    13681 ttaatagaga tgacaagaca gataagctca gttaattact tatatatctt tttaatatgg
    13741 tatatttgtc aacaaaaaga aatcaaatca taccttatac tcaatgaaaa tcaaagagca
    13801 aactaggaag ctagccgcaa gttgctcaaa acactgtttt gaggttgcag atagagctaa
    13861 cgtggtttga agagattttc gaagagtatt aaagacagtg tgactatgct tgtcaataga
    13921 taggaaatca aaatgaagga tggaaatctg agccaaacat cataagtgct ataatgaaca
    13981 gaagtaagaa ttgcacgata agttttctaa aaccatagga atcgtcccct ttcttcatct
    14041 tcattatagc atatatacat cagttgtaca aataatatga tattttttta ttagtcctca
    14101 gacaagcata ttataaagcg tttcattacc atttaagtta atataagctg gatcaaaacc
    14161 ttccattcta cgaatcaaac ctgcataatc atgcttatct gctaaagcga tcccaatcaa
    14221 tactgggcct gttcccttgc tagctcgttt gatatactca aaacgtgtga tatcatcatt
    14281 tggccccagg atatcattta caaactcacg caaagctcct ggacgttgtg ggaaattgac
    14341 cacaaaataa tgtttgatac catcataaat caaggcacgc tcttccattt ctggcatacg
    14401 gttgatatca ttatttcctc cagaaatgat acaacaaatg gttttcccct taatatattc
    14461 agctaaaacc tctaaagagg cgatactagc tgctccagca ggttctgcga ctatcccttg
    14521 cttagagtaa aggtcaatca aggtttcaga aatcaatccc tcatcgacac ctaccaaagt
    14581 ttgaatatgt tgacgagttg cttcataggt caactgacct accttttgca cagcaatccc
    14641 atccgcaaat ttatcaattt ccttgagttt gactggacct ccagcctcaa aggcagcttt
    14701 catggaacgc gctccattcg cctctactcc gataacctca atctctggac ttgtttcctt
    14761 gatataggta gaaaccccgg caatgagacc gccaccacca acaggaacca agacagcatc
    14821 aaaatcaatc gattcttttc gagcttcttc taaaatctca taagcaactg ttccttgacc
    14881 tgcttgaaca tgagcatcat caaaaggatc aataaaggta cgattttcag agactgtaaa
    14941 ttcttgagct gctttggctg aggcatcaaa ggtatctcca actagtttaa tagttacaaa
    15001 atccccacca aaaaagcgaa cctgaccaat cttttgttgt ggcgtagtaa tgggcataaa
    15061 gatagtagca ggaattttca tttcattaca agtataggct actccctgcg catgatttcc
    15121 cgcagaagcg caaactaccc cacgttcacg ttcttccttg ctgagctggg aaatggcata
    15181 ataggcacca cgaattttaa aggagcgaac acgctgggca ttttcttttt tcaaataaat
    15241 cttagcacca tacttctccg ataaataatg atcgtaatcc agtggagtat tcacaaccac
    15301 accgttcaag accttgtgag ccttgattat atcttttgaa cttaacatat aaaacctctt
    15361 tttctataaa aaacagtcac taatgactgt tttttagtta ttcatctccg aaaatttcag
    15421 taacactttc atggtattta tgagccatac gaatcgaaaa ctctttagga agttcataac
    15481 gatttgtgga aatccaatca tataaccatt ctaccaaact ttcttcacta tcattacgat
    15541 ggaagataaa atagtttaaa atttctttat attctacatc attcaaatcc aacgtgtcta
    15601 caaattgact acgaggaaca aaattagaat ctaattttct tagttgatct gtcaattctc
    15661 ccaattgtaa atccaactga aaaaatagtt tttgcagttg cctatcagtt tcatcacatc
    15721 ctgtcttgat agaaaacaaa ttggtaatat tcatatgatt tcaaccccta aataagtaca
    15781 tctaatatcc cacgaatagc tgaagctaaa gttttcgcag tccagtcatt taaacccgat
    15841 tctttcaatc cagatgcaat aatttgctct atttgatata aaccgccatc tatcgtctca
    15901 tttattctgc gaagaggacc ttcaatatag gataaagctt ctccgaatct aacagctgat
    15961 ttaggtccta atgatttacc aactatattt gttaatattt tttatttctc aaaatccatt
    16021 tcgctatata acgagcagat ttagtcgcac cagctctctc aaacggagaa tgaatttcaa
    16081 gaccttcttc tctagctatt tgtgccaatt tgaagaattg ttcaaaggtc atatgcaacc
    16141 taccatcttc aaaattagca tcatcataaa tgttaattac agcattttca tcatacgacc
    16201 aatctgcttt attttggtct gctagcacta ttaccgacgg tgtaattaca ttatacaact
    16261 agaaccaata ataacgatgc aatttttctc ataatctttt tcataaaatc atctccagcg
    16321 ctatatattt ctaattagtt atagattttg aatgcatcat cgtcgttttt accaacgaat
    16381 ggcattgctt tacgcaattc tgcaccaact ttttcaattt caaggttagc tgcttgttca
    16441 cggtaagcag tcaattttgg acgtccagct ttatagtcat ttacaaagtc atttgcaaat
    16501 ttaccatttt ggatgtctgc caagacagcc ttcatatttt ctttaacttg ttcagtgatt
    16561 acacgtggac ctgatacata gtcaccgtat tcagcagtgt ttgaaataga ttgacgcatt
    16621 ttcttgaatc caccttcgta gatcaagtca acgatcaatt tcatttcgtg aagaacttca
    16681 aagtaagcca attctggagc gtaacctgct tctgtcaaga cttcgaaacc tgcttcgata
    16741 agggcagtca aaccaccaca aagtacagct tgttcaccaa acaaatcttc ttcagtttct
    16801 tctttgtaag ttgtttcaag aagacctaca cgagccgctc caacaccttt acaccagtcc
    16861 atagcaatgt ttttagcatt tcctgttgca tcttggtata ctgcataaag agctggaaca
    16921 ccaaatcctt cttcgtaagt acgacgtacc aagtgtcctg gtcctttagg agcacacatg
    16981 aagacatcta catccgcagg aactttgata aattcaaagt ggatgttgaa accatgggca
    17041 aatccaactg cgtttccagc ttccaagttt ggagcgattt ctgcttcgta caattcttgt
    17101 tgaatttcgt ctggcgccaa gatcatgata acatcagcca acttagtagc ttctgctact
    17161 gtgtaagtat caaatccatc ttcttttgct ttatcaaaag atttacctgg acgtacaccg
    17221 ataataacgt cacgacctga atcacgcaag ttttgagcat gcgcatgccc ttgtgaacca
    17281 taaccgataa cggcgatttt tttaccgtca agtgctgcta ctttaacatc tttttcatat
    17341 tccatttgaa ctgtcatagc ttttctctct tttctattat ttattgcctt ttaagctggt
    17401 ttaacaaatt taagttggat ttttaatcgc gggtaaatcc agttgcaccc gttcgagcaa
    17461 tattgcgaat accgtatggg cgaatgactc gcaatagggc ttcgctcttt tctgcatttc
    17521 ccgtcatctg aatggtaatc gagcttggcg ctacgtctac cactgttgca cggaaaggtt
    17581 gaataatcgc taaaatctca gctctcttct cagctggcgc tgacatctta accaaaatca
    17641 cctcgcgctc caaatgaggc ttgtctgtaa tatctcgaat gcgaatcaca tcaatctgac
    17701 gattgagctg tttgatgatt tgctccactt catcatgaga agcaacatca ataataatag
    17761 tgatacgcga tacattcgga tcttctgttg ctccaacaga gatgctttca atattaacct
    17821 gacgacgaga taggacacct gtaaagcgat tgaggactcc tgaacgattt tgtagttttg
    17881 ctgttaacat tctacgcatg gaactgcacc cccaacatct catgattact cttaccagcc
    17941 ggtaccattg gtaacacctg ttccttacga gaaatatcta cctcaattag cataggaaca
    18001 tcctcagtga tgacttcaag gtcttgagcc aaggtctcag gattgtcaaa cttatagttt
    18061 ttaataccat aagcctgcgc catcaattgg aaatcaggaa gggtatcaaa gaccgactct
    18121 gatgttctgc cttcatagaa ggattcctgc cactggcgaa ccattccgag tgaatgattg
    18181 ttcagcataa ccaccttgat tggcaccttg taaatattca aaatagccaa ctcctggttg
    18241 gtcatttgga aaccaccatc cccaacaaac aagactactt ccttatctgg gttagcaatt
    18301 ttagcaccga ttgctgctgg aattccaaag cccattgttc ccaaaccacc tgaagtcact
    18361 aactgacgtt cattttggta gggataatac tgagctgtcc acatttggtg ttgaccaacg
    18421 tctgttacca caatggcatc tccattcgtc aattcaccaa ttcgttcaat aactgcttgc
    18481 ggttgaacca cacgctcttt cttgtcatac gaacgaacac gattcttgtc tttagtgact
    18541 ttctcaatcc acttttcagt attgttgtga actgttggtt ctgctagcaa catttgcaag
    18601 gccttcttag catctccaac tacaggaatg tctgcactga taatcttgcc aatctcagct
    18661 gggtcaatat caatgtgggc aaccttagca ttcttagcga aagtcttagg attccccgtc
    18721 aaacggtcat cgaaacgaga accaatacta atcataaagt ccgcttccgt catggcaata
    18781 tttgctgcga atgacccgtg catgcctccc attccaagaa agagtgggtg actcgttgca
    18841 atcgttcctt gtcccaaaag actggttacc actggaattt gatagcgttc tgcaaattca
    18901 tttagttccg tagcagcctc agcataacta attccaccac cagctaacaa gactggcttt
    18961 ttagccttgg acaattgctt caagattttc ttgatttgca tatcattcgg ctcaagagtc
    19021 ggctgataac ttggtaaatt cacttctggt gaataaatga agtctgtttc taaagcagat
    19081 atgtcttttg gtaggtcaat tacaactggc cctggacggc ctgtagttgc gatatggaca
    19141 gcttccgtaa tgatacgcgg aatatcagct gtctcacgaa cttggtaatt gtacttagtg
    19201 attggcatgg taattcctac gatgtctgcc tcctgaaagg catccttccc aatccctgct
    19261 cgcgccacct gacctgtaaa gaccaaaagg ggaacgctat cgctcatagc atccgcaatc
    19321 cctgtaatgg catttgttgc tcctggtcca ctagtgacga cggcaacacc caactttcca
    19381 gttgatttgg catagccttc agcttcatgc aaacaacctt gctcatggcg ccctagaatg
    19441 tggcgaatgc ctttaaaatt atatatcgca tcataaaaag gcaaaaccgc accaccagga
    19501 taaccaaaga tggtatcaac tcctaaatca cgaagtgttt ccaaaactag gtccgacccc
    19561 gtcttaggag attctaaact gattttctcc attattcccc tttctt
//
LOCUS       DAVLFB010000031        18320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLFB010000031 DAVLFB010000000
VERSION     DAVLFB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.031"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            91..5394
                     /locus_tag="VXD58_001006"
     CDS             91..5394
                     /locus_tag="VXD58_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0675725.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDERDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KQALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEITVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASVTVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSNQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(5561..7720)
                     /locus_tag="VXD58_001007"
     CDS             complement(5561..7720)
                     /locus_tag="VXD58_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV0675726.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VXD58_001008"
     CDS             complement(7717..8313)
                     /gene="recU"
                     /locus_tag="VXD58_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV0675727.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8379..8906
                     /locus_tag="VXD58_001009"
     CDS             8379..8906
                     /locus_tag="VXD58_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV0675728.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            8976..9305
                     /gene="gpsB"
                     /locus_tag="VXD58_001010"
     CDS             8976..9305
                     /gene="gpsB"
                     /locus_tag="VXD58_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV0675729.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9326..9706
                     /gene="rnpB"
                     /locus_tag="VXD58_001011"
     ncRNA           9326..9706
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VXD58_001011"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9791..10948
                     /locus_tag="VXD58_001012"
     CDS             9791..10948
                     /locus_tag="VXD58_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV0675730.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREMRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLCSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10961..12355
                     /gene="mapZ"
                     /locus_tag="VXD58_001013"
     CDS             10961..12355
                     /gene="mapZ"
                     /locus_tag="VXD58_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV0675731.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12431..13855
                     /gene="gndA"
                     /locus_tag="VXD58_001014"
     CDS             12431..13855
                     /gene="gndA"
                     /locus_tag="VXD58_001014"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV0675732.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPYLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13867..14556
                     /locus_tag="VXD58_001015"
     CDS             13867..14556
                     /locus_tag="VXD58_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675733.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGIGYTMQE"
     gene            14654..15676
                     /gene="cbpC"
                     /locus_tag="VXD58_001016"
     CDS             14654..15676
                     /gene="cbpC"
                     /locus_tag="VXD58_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV0675734.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            15694..16692
                     /gene="cbpJ"
                     /locus_tag="VXD58_001017"
     CDS             15694..16692
                     /gene="cbpJ"
                     /locus_tag="VXD58_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV0675735.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(16810..18042)
                     /locus_tag="VXD58_001018"
     CDS             complement(16810..18042)
                     /locus_tag="VXD58_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0675736.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
BASE COUNT         5658 a         3312 c         4228 g         5122 t
ORIGIN      
        1 cagggttaag cattgcactt tcagaggaag gagtattttt taaaaaggaa atgtaaacgc
       61 ttgcccaaaa atgaaaggat ttagaagttt atgaataaag gattatttga aaaacgttgt
      121 aaatatagta ttcggaaatt ttcattaggt gttgcttctg ttatgattgg agctgcattc
      181 tttgggacaa gtccggttct tgcagatagc gtgcagtctg gttctacggc gaatttacca
      241 gctgatttag ctactgctct tgcaacagca aaagagaatg atgagcgtga ttttgaagcg
      301 cctaaggtgg gagaagacca aggttctcca gaagttacag atggacctaa gacagaagaa
      361 gaactattag cacttgaaaa agaaaaaccg gctgaagaaa aaccaaaaga ggataaacct
      421 gcagctgcta aacctgaaac acctaagacg gtaacccctg aatggcaaac ggtagagaaa
      481 aaagaacaac aaggaacagt cactatccga gaagaaaaag gtgtccgcta caaccaacta
      541 tcctcaactg ctcaaaatga taacgcaggc aaacaagccc tgtttgaaaa gaagggcttg
      601 accgttgatg ccaatggaaa tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag
      661 ggcaaatcac gctttggtgt cttcttgaaa tttaaagata ccaataataa tgtttttgtc
      721 ggttatgaca aggatggctg gttctgggag tataaatctc caacaactag cacttggtat
      781 agaggtagtc gtgttgctgc tcctgaaaca ggatcaacaa accgtctctc tatcactctc
      841 aagtcagacg gtcagctaaa tgccagcaat aacgatgtca atctctttga cacagtgact
      901 ctaccagctg cggtcaatga ccatcttaaa aatgagaaga agattcttct caaggcgggc
      961 tcttatgacg atgagcgaac agttgttagc gttaaaacgg ataaccaaga gggggtaaaa
     1021 acagaggata cccctgctga aaaagaaaca ggtcctgaag ttgatgatag caaggtgact
     1081 tatgacacga ttcagtctaa ggtcctcaaa gcagtgattg accaagcctt ccctcgtgtc
     1141 aaggaataca gcttgaatgg acatactttg ccaggacagg tgcaacagtt caaccaagtc
     1201 tttatcaata accaccgaat cacccctgaa gtcacttata agaaaatcaa tgagacaaca
     1261 gcagagtact tgatgaagat tcgcgatgat gctcacttaa tcaatgcgga aataacagta
     1321 cgcttgcaag ttgtggacaa tcaattgcac tttgatgtga ccaagattgt caaccacaat
     1381 caagtcactc caggtcaaaa gattgatgac gaaagaaaac tactttcttc tattagtttc
     1441 ctcggcaatg ctttagtctc tgtttctagt gatcaaactg gtgctaagtt tgatggggca
     1501 accatgtcaa acaatacgca tgtcagcgga gatgatcata tcgatgtaac caatccaatg
     1561 aaggatttgg ctaagggtta catgtatgga tttgtttcta cagataagct tgctgctggt
     1621 gtttggagta actctcaaaa tagctatggt ggtggttcga atgactggac tcgtttgaca
     1681 gcttataaag aaacagtcgg aaatgccaac tatgtaggaa tccatagctc tgaatggcaa
     1741 tgggaaaaag cttataaggg cattgttttc ccagaataca cgaaggaact tccaagtgct
     1801 aaggttgtta tcactgaaga tgccaatgca gacaagaaag ttgattggca agatggtgcc
     1861 attgcttatc gtagcattat gaacaatcct caaggttggg aaaaagttaa ggatatcaca
     1921 gcttaccgta tcgcgatgaa ctttggttct caagcacaaa acccattcct tatgaccttg
     1981 gatggtatca agaaaatcaa tctccacaca gatggtcttg ggcaaggtgt tctccttaaa
     2041 ggatatggta gcgaaggcca tgactctggt cacttgaact atgctgatat tggtaagcgt
     2101 atcggtggtg tcgaagactt caagacccta attgagaagg ctaagaaata tggagctcat
     2161 ctaggtatcc acgttaacgc ttcagaaact tatcctgagt ctaaatactt caatgaaaaa
     2221 attctccgta agaatccaga tggaagctat agctatggtt ggaactggct agatcaaggt
     2281 atcaacattg atgctgccta tgacctagct catggtcgtt tggcacgttg ggaagatttg
     2341 aagaaaaaac ttggtgacgg tctcgacttt atctatgtgg acgtttgggg taatggtcaa
     2401 tcaggtgata acggtgcctg ggctacccac gttcttgcta aagaaattaa caaacaaggc
     2461 tggcgctttg cgatcgagtg gggccatggt ggtgagtacg actctacctt ccatcactgg
     2521 gcagctgact tgacctacgg tggctacacc aataaaggta tcaacagtgc catcacccgc
     2581 tttatccgta accaccaaaa agatgcttgg gttggggact acagaagtta tggtggtgca
     2641 gccaactatc cactgctagg tggctacagc atgaaagact ttgaaggctg gcaaggaaga
     2701 agtgactaca atggctatgt aaccaactta tttgcccatg acgtcatgac caagtatttc
     2761 caacacttca ctgtaagcaa atgggaaaat ggtacaccgg tgactatgac cgataacggt
     2821 agcacctata aatggactcc agaaatgcga gtggaattgg tagatgctga caataataaa
     2881 gtagttgtaa ctcgtaagtc aaatgatgtc aatagtccac aatatcgcga acgtacagta
     2941 acgctcaacg gacgtgtcat ccaagatggt tcagcttact tgactccttg gaactgggat
     3001 gcaaatggta agaaactttc tactgataag gaaaagatgt actacttcaa tacgcaggcc
     3061 ggtgcaacaa cttggaccct tccaagcgat tgggcaaaga gcaaggttta cctttacaag
     3121 ctaactgacc aaggtaagac agaagagcaa gaactaactg taaaagatgg taaaattact
     3181 ctagatcttc tagcaaatca accatacgtt ctctatcgtt cgaaacaaac caatcctgaa
     3241 atgtcatgga gcgaaggcat gcacatctat gaccaaggat ttaacagtgg taccttgaaa
     3301 cattggacca tttcaggcga tgcttctaag gcagaaattg tcaagtctca aggggcaaac
     3361 gatatgcttc gtattcaagg aaacaaagaa aaagttagtc tcactcagaa attaactggc
     3421 ttgaaaccaa ataccaagta tgccgtttat gtcggtgtcg ataaccgtag taatgccaag
     3481 gcgagcgtca ctgtaaatac tggtgaaaaa gaagtgacta cttataccaa taagtctctc
     3541 gccctcaact atgtaaaagc ctacgcccac aatacacgtc gtaacaatgc tacagttgac
     3601 gatacaagtt acttccaaaa catgtacgcc ttctttacaa ctggatcgga cgtatcaaat
     3661 gttactctga cattgagtcg tgaagctggt gatcaagcaa cttactttga tgaaattcgt
     3721 acctttgaaa acaattcaag catgtacgga gacaagcatg atacaggtaa aggcaccttc
     3781 aagcaagact ttgaaaatgt tgctcagggt atcttcccat ttgtagtggg tggtgtcgaa
     3841 ggtgttgaag ataaccgcac tcacttgtct gaaaaacaca atccatatac acaacgtggt
     3901 tggaatggta agaaagtcga tgatgttatc gaaggaaatt ggtcactcaa gacaaatgga
     3961 ctagtgagcc gtcgtaactt ggtttaccaa accatcccac aaaacttccg ttttgaagca
     4021 ggtaagacct accgtgtaac ctttgaatac gaagcaggat cagacaatac ctatgctttt
     4081 gtagtcggta agggagaatt ccagtcaggt cgtcgtggta ctcaagcaag caacttggaa
     4141 atgcatgaat tgccaaatac ttggacagat tctaagaaag ccaagaaggc aaccttcctt
     4201 gtgacaggtg cagaaacagg cgatacttgg gtaggtatct actcaactgg aaatgcaagt
     4261 aatactcgtg gtgattctgg tggaaatgcc aacttccgtg gttataacga cttcatgatg
     4321 gataatcttc aaatcgaaga aattacccta acaggtaaga tgttgacaga aaatgctctg
     4381 aagaactact tgccaacggt tgccatgact aactacacca aagagtctat ggatgctttg
     4441 aaagaggcgg tctttaacct cagtcaggcc gatgatgata tcagtgtgga agaagcgcgt
     4501 gcagagattg ccaagattga agctttgaag aatgctttgg ttcagaagaa aacggctttg
     4561 gtagcagatg actttgcaag tcttacagct cctgctcagg ctcaagaagg tcttgcaaat
     4621 gcctttgatg gcaatgtgtc tagtctatgg catacatctt ggaatggtgg agatgtaggc
     4681 aagcctgcaa ccatggtctt gaaagaacca actgaaatca ctggacttcg ttatgttcca
     4741 cgtggatcag gttcaaatgg taacttgcgt gatgtgaaac ttgttgtgac agatgagtct
     4801 ggcaaggagc atacctttac tgcaactgat tggccagata acaataagcc aaaagacatt
     4861 gattttggta agacaattaa ggctaagaaa attgtcctta caggtactaa gacttacgga
     4921 gatggtggcg ataaatacca atctgcagcg gaacttatct ttactcgtcc acaggtagca
     4981 gaaacacctc ttgacttgtc aggctatgaa gcagctttgg ctaaggctca gaaattaaca
     5041 gacaaagaca atcaagagga agtagctagc gttcaggcaa gcatgaaata tgcgacggat
     5101 aaccatctct tgacggaaag aatggtggaa tactttgcag attatctcaa ccaattaaaa
     5161 gattctgcta cgaaaccaga tgctccaact gtagagaaac ctgagtttaa acttagctct
     5221 ttagtttcca atcaaggtaa gacgccagat tataagcaag aaatagatag accagaaaca
     5281 cctgaacaaa tcttgccagc aacaggtgag agtcaatctg acacagccct cttcctagca
     5341 agtgttagtc tagccctatc tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta
     5401 gtaaaacctc ttaacaagat tacggaagca gtctctatct tttccaatga ggtttatagt
     5461 acagaaaaag cctgagaaga tgtcttctca ggcttttgtt aagcacataa atacaatagt
     5521 gctatgacaa aatcacccag aaaaatctgg gtgataaatg ttatggttgt gctggttgag
     5581 gattctgatt ttgttgatca ggggttgtat ttgattgttg cgtattattg ttaggattgg
     5641 tagtcgtact attatttgtg cttggagtgg ttgagctaga ctgtgaagtt gaactatctg
     5701 atgatgagct tgaactttca gttgatgggg gttgttgtgg agcaggtgag ttccacgtag
     5761 aacgagcacc atttttaaat acgaattctc catttctgta gagcccctct ggtatattcc
     5821 aatcctctgg attgcttcct tcagacagat aggtcatcat agagcggtaa actttggcag
     5881 cgaccgtaag gccattgcct acaagtggtg tcagacggtt agaatagcct gtccatacag
     5941 ccattgaata tttacgcgta tagccagcaa atagttcatc aggtgctaca aattgagagg
     6001 tcttgatgtg gttttcaatt tcctcgtctg tatagttaga ggttcctgtt ttaccagcct
     6061 gagggagcca agcaagatag gcatttcgtc cagttccata actcaagact gttttcatca
     6121 tgtcggtcat catataggct gtcgtttcct tcatggcacg agttccgaca ttagagaact
     6181 ctttttcact cccatcacta aagacgactt tatggatata cattggttta tagtaagttc
     6241 caccatttgc aaaggcagcg taagcagcag ccatcttttc actacttgct ccatattttt
     6301 tgtctgattc ggttgtgtta cttgaaatgg catttgagta gtgaatactt gggtagtcga
     6361 ttccgagacc atttaggaaa gtcttggcgc ggttgagtcc gaccttgttt agagtttcca
     6421 cggctgggac gtttcgcgat tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc
     6481 ccctatccca gttataaaca ggggtatttg tcccagggta gttatagggc tcatcgtgaa
     6541 cgatagtagc agttgaatcg tagataccgt actccaaggc aggagcatag tctgtgatcg
     6601 gtttcatagt tgatccccag tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa
     6661 cattacttga ctgatggcgt gctcctagct gggcaatgac tttaccgtta gaaacatcaa
     6721 caatggtaga agcgacttgc aattcatcgt ctggataggc aacgtattcg tctgtattgt
     6781 aaatatccca cagatgtttt tgagcttctt ggtctacatt tgtgtagaca tccatcccag
     6841 ttgtgagcag gttatagcct gtttcttctt caacttgatt gatgacttcc ttgaggtaat
     6901 tatccatgta agcagggtaa ttacttgctg atttgagact ttgtagtcca tcagtaattg
     6961 gtgtattgac tgctttctca tactgttcag cagagatgta gccttgattt ttcatttcag
     7021 ataagaccaa gtttcggcgg tcttgggctg cttctggatg tgaatagggg tcatattggt
     7081 ttggtgcctg aggcattcca gccagcaagg ctaactgagg taaacttaaa ttattgaggt
     7141 ctttaccata gtagttttga gctgctgtct gcattccata gttcccatta gacatgtaga
     7201 ccttatttat atagtaggtc aagatttctt gcttggttgc tttttgttct aactgaatcg
     7261 ctaaccaagc ttcctgagcc ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt
     7321 aagtcaactt aatcaactgt tgggtgagag ttgatccacc ttggagggaa ttgctttgca
     7381 gattgcgcaa gaaagctcct aggatacgga tggtatcaat ccccctgtgg tcgaagaagc
     7441 gatggtcttc gatagaaacg attgccttaa ccaaatctgt gggaatatca ttagcttggg
     7501 cattgacgcg gcgttcagaa cccaagtcag caatgagttg atttttattg tcgtagattt
     7561 tactagaagt tgttgcaact agtttactct cggataggct aggagccttg ctaacgtagt
     7621 agaaaaaaac tcctccgcct aagacaatgg ctgcgataac caagcttaag aagctaatgc
     7681 tcagatactt gattaggcgc agaatcgttg gtttgttcat cttgttttac cacctaataa
     7741 atgttctttg ataacattga gataaggaat ttgagggaag gcaccagcct tgatttcata
     7801 tccatattct cgaatatatt caagtggcat tgatttttgt cccttatctt gatgatagaa
     7861 gcgaatcaaa tcgaatgccg gcaataagta ggtttcttgc tgagaagaaa agtgaagaag
     7921 gacaaagcag attccttgtt gggcaaggac ttgttccata tgctgaatct gatgtggatg
     7981 aaaatttttc atcggaatcg cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta
     8041 atatccatta taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac
     8101 aatcttggca cgacttcgtt gtggatagtc cacttgtacg atttgaatag gagttggttt
     8161 cttatgtata acagccaagc cctgagacaa atagtagtcg ttggtagcat tgatcatctt
     8221 ttcaaaagac attcctcgat ttgcgaaatt tttgggttga gaaagagatg tttgtctttt
     8281 ttgtgatgaa actttatgtg gatagttgac cataattctc cttattggta caataacatc
     8341 actttattat accataattt gcacagaaag ggttaaaaat ggctacagct ttggttttag
     8401 gctattctgc ttttgatttg ggtttatttt ctgataagga tcctcgtctt aaattgatta
     8461 aaaaagcgat acgtaaagat ttagaggcta tggcagcaga tggggtgtct tggcttgtgt
     8521 ttacagggag tttgggtttt gaatattggg ttttagaggt tgctcaggag atgaaaactg
     8581 aatacggttt ccagttggcc accatttttg cttttgaaac ccatggggaa aattggaatg
     8641 aaggcaatca gatgaaactg agtcgcttta agcaggtaga ctttgtcaaa tatgcctatc
     8701 ctcgctatga acacaagggg cagttaagag attaccagca gtttttgctg gaaaacacga
     8761 ctagttccta tcttttttat gatgaagaaa atgaaacaaa actagcttat ttttaccaaa
     8821 agatgaaaaa tcaagaggac tattttataa agagattaac atttgatgaa ttaaatgaac
     8881 ttgctgaaaa tttttccgaa aattgaagct ttgaccttga tttttgtttg ccttttttta
     8941 tataataata ctagcaagcg agaatggaga gagacatggc aagtattatt ttttcagcga
     9001 aagatatttt tgaacaagag tttggacgtg aagtccgtgg ctataataaa gtagaagttg
     9061 acgagttttt agacgatgtc atcaaggact atgaaaccta tgctgccttg gtcaagtcac
     9121 ttcgtcagga aattgcggat ttgaaggaag aattaactcg taaaccgaaa ccttcaccag
     9181 ttcaagcaga accccttgaa gcggcaatta caagttctat gacgaatttt gatattttga
     9241 aacgcctgaa tagattggaa aaagaagttt ttggtaaaca aattttagat aactcagatt
     9301 tttaagtagt tatttgagat gtgcaatttt tggataatcg cgtgaggaga attgcttctc
     9361 atgaggaaag tccatgctag cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac
     9421 cataagccta gggacgagaa atcgttacgg cagttgaaat ggctaagtcc ttggataggc
     9481 cagagtaggc ttgaaagtgc cacagtgacg gagtctttct ggaaacagag agagtggaac
     9541 gcggtaaacc cctcaagcta gcaacccaaa ttttggtcgg ggcatggagt acgcggaaac
     9601 gaacgtagta ttctgactgc tatcagctag agctgttagt ggtagacaga tgattatcga
     9661 aggaagtggt cctagtcact tctggaacaa aacatggctt atagaaaatt gcatataggt
     9721 tggggctgag aaattttctc aacctcattt tttaaagtgg acatatagaa aggtcttgca
     9781 agactgtaac atgaaaaaag aatttaattt aattgcaact gtggcagcag ggcttgaggc
     9841 tgtcgttggt cgtgaaatgc gagagttggg ctacgattgt caggttgaaa atggacgtgt
     9901 tcgttttcaa ggagacgtga gagctattat cgaaaccaac ctttggcttc gggcagcaga
     9961 tcgtatcaaa attatcgtag gaacgttccc agctaagact tttgaagagc tatttcaggg
    10021 agttttcgct ttagattggg aaaattattt accacttgga gctcggttcc cgatttcaaa
    10081 agctaaatgt gttaagtcca aacttcacaa tgagcccagt gttcaggcta tttctaagaa
    10141 agctgttgtc aagaaattgc agaaacacta tgctcgccca gaaggggttc ctctgatgga
    10201 gaatggtcca gagtttaaga ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat
    10261 tgatacgacc gggtctagcc tctttaaacg tggttatcgt accgaaaaag gtggcgctcc
    10321 tatcaaggaa aatatggcag cagccatttt acaactttct aactggtatc cagacaagcc
    10381 tttgattgat ccgacctgtg gttcggggac tttctgtatt gaggcagtta tgattgctag
    10441 aaagatggcg ccaggtcttc gtcgttcttt tgcatttgag gaatggaact ggatcagcga
    10501 tcgcttgatt caagaagtgc gcacagaagc ggctaaaaaa gtagaccgtg agcttgagct
    10561 ggatatcatg ggctgtgata ttgatgctcg catggtggaa attgctaagg ccaatgctca
    10621 ggtagctggt gttgcaggag acattacttt taagcagatg cgcgtgcagg atttatgttc
    10681 cgataaaatc aatggagtaa tcatttccaa tccgccttat ggtgaacgtt tgtcagatga
    10741 tgcaggggtg accaagctct atgctgagat ggggcaagta tttgcaccgc tgaaaacttg
    10801 gagcaaattt atcctgacta gtgatgaagc ttttgaaagc aagtatggta gccaagcaga
    10861 taagaagcgt aagttataca acggaacctt gaaagtggat ctatatcaat attttggtca
    10921 gcgtgtcaaa cggcaagagg taaaatagaa agggatactc atgagtaaaa aaagacgaaa
    10981 tcgtcataaa aaagaaggtc aagaaccgca atttgatttt gatgaagcaa aagagctaac
    11041 agttggtcaa gctattcgta aaaatgaaga agtggaagca ggagtcttgc ctgaggattc
    11101 cattttggac aagtatgtta agcaacacag agatgaaatt gaggcggata agtttgcgac
    11161 tcgtcaatac aaaaaagagg agttcgttga aactcagagt ctggatgatt taattcaaga
    11221 gatgcgtgag gctgtagaga agtcagaagc ttcttcggag gaagttccat cttctgaaga
    11281 catcttacta cccttgcctc tggacgatga ggagcaaggc ttggatcctc tattgctaga
    11341 tgatgaaaat ccaacagaaa tgactgaaga agtggaagag gagcaaaacc tttctcgtct
    11401 ggatcaagag gactcagaaa agaaaagtaa aaaaggcttt attttgaccg ttttggcgct
    11461 tgtatcagta attatttgtg tcagtgctta ttatgtctac cgtcaagtgg ctcgttcgac
    11521 taaggaaatt gaaacttctc aatcaactac agccaatcaa tcggatgtgg atgattttaa
    11581 tacactttat gacgcctttt acacagatag caataaaacg gctttgaaaa atagccagtt
    11641 tgataaactg agtcaactca agactttact tgataagctg gaaggtagtc gtgaacatac
    11701 gcttgccaaa tctaaatatg atagtctagt aacgcaaatt aaggccattc aagatgtcaa
    11761 tgcacaattt gaaaaaccag ctattgtgga tggtgtgttg gataccaatg ccaaagccaa
    11821 atcggatgct aaatttacag atattaaaac tgggaatacg gagcttgata aagtgctaga
    11881 taaggctatc agtcttggta agagccagca aacaagtact tctagctcaa gttcaagtca
    11941 aactagcagc tcaagctcta gtcaagcaag ctcaaatacg actagtgagc caaaaccaag
    12001 tagttcaaat gagactagaa gtagtcgcag tgaagtcaat atgggtctct cgagtgcagg
    12061 ggttgctgtt caaagaagtg ccagtcgtgt tgcctataat cagtctgcta ttgatgatag
    12121 taataactct gcctgggatt ttgcggatgg tgtcttggaa caaattctag caacttcacg
    12181 ttcacgtggc tatatcactg gagaccaata tatccttgaa cgtgtcaata tcgttaacgg
    12241 caatggttat tacaacctct acaagccaga tggaacctat ctctttaccc ttaactgtaa
    12301 gacaggctac tttgtcggaa atggcgctgg tcatgcggat gacttggatt actaagcagt
    12361 cgttacaaaa ttctttcttt tcaaaagtaa aaatgataaa ataaaacaaa tgaaacaaga
    12421 ggagtgtcaa atgacaaaag ctaactttgg tgttgtaggt atggccgtaa tgggtcgtaa
    12481 ccttgccctt aatattgaat cacgtggtta cacagttgct atctacaacc gtagtaaaga
    12541 aaaaacggaa gatgtgattg cttgccatcc tgaaaagaac tttgtaccaa gctatgacgt
    12601 tgaaagtttt gtaaactcaa tcgaaaaacc tcgtcgtatc atgctgatgg ttcaagctgg
    12661 acctggtaca gatgctacta tccaagccct tcttccatac cttgacaagg gtgatatctt
    12721 gattgacggt ggaaatactt tctacaaaga taccatccgt cgtaatgaag aattggcaaa
    12781 ctcaggtatc aactttatcg gtactggggt ttctggtggt gaaaaaggtg cccttgaagg
    12841 tccttctatc atgcctggtg gacaaaaaga ggcctacgaa ttggttgcgg atgttcttga
    12901 agaaatctca gctaaagcac cagaagatgg caaaccatgt gtgacttaca tcggccctga
    12961 tggagctggt cactatgtga aaatggttca caatggtatt gagtacggtg atatgcaatt
    13021 gatcgcagaa agctatgact tgatgcaaca cttgctaggc ctttctgcag aggatatggc
    13081 tgaaatcttt actgagtgga acaagggtga attagacagc tatttgatcg aaatcacagc
    13141 tgatattttg agccgtaaag acgatgaagg ccaagatgga ccaatcgtag actacatcct
    13201 tgatgctgca ggtaacaagg gaactggtaa atggactagc caatcatctc ttgaccttgg
    13261 tgtaccattg tcactgatta ctgagtcagt gtttgcacgc tacatttcaa cttacaaaga
    13321 agaacgtgta catgctagca aggtgcttcc aaaaccagct gccttcaact ttgaaggaga
    13381 caaggctgaa ttgattgaaa agatccgtca agccctttac ttttcaaaaa tcatttcata
    13441 cgcacaagga tttgctcaat tacgtgtagc ctctaaagaa aacaactgga acttgccatt
    13501 tgcagatatc gcatctatct ggcgtgatgg ctgtatcatc cgttctcgtt tcttgcaaaa
    13561 gattacagat gcttacaacc gtgatgcaga ccttgccaac cttcttttgg atgaatactt
    13621 cttggatgtt actgctaagt atcaacaagc agtgcgtgat atcgtagctc ttgcggttca
    13681 agctggtgtg ccagtgccaa ctttctcagc agctattact tactttgata gctaccgttc
    13741 agctgacctt ccagctaact tgatccaagc acaacgtgac tactttggtg ctcacactta
    13801 ccaacgtaaa gacaaagaag gaaccttcca ctactcttgg tatgacgaaa aataagtagg
    13861 tcagccatgg ggaaacggat tttattactt gagaaagaac gaaatctagc tcatttttta
    13921 agtttggaac tccagaaaga gcagtatcgg gttgatctgg tagaggaggg gcaaaaagcc
    13981 ctctccatgg ctcttcagac agactatgat ttgatgttat tgaacgttaa tctgggagat
    14041 atgatggctc aggattttgc agaaaaattg agccgaacta aacctgcctc agtcatcatg
    14101 attttagatc attgggaaga cttgcaagaa gagctggaag ttgttcagcg ttttgcagtt
    14161 tcatacatct ataagccagt ccttatcgaa aatctggtag cgcgtatttc ggcgatcttc
    14221 cgaggtcggg acttcattga tcaacactgc agtctgatga aagttccaag gacctaccgc
    14281 aatcttagga tagatgttga acatcacacg gtttatcgtg gtgaagagat gattgctctg
    14341 acccgccgtg agtatgacct tttggcgaca cttatgggaa gcaagaaagt attgactcgt
    14401 gagcaattgt tggaaagtgt ttggaagtat gaaagtgcga ccgagacaaa tatcgtagat
    14461 gtctatatcc gctatctacg gagcaagctt gatgttaaag gacaaaaaag ctacattaaa
    14521 actgtgcgtg gtattggata taccatgcaa gaatagaaaa gcagttgcag ttgtgtaact
    14581 gcttttttga ggagttttta tatattgaca tgcagtcggg tctttgctac aatcgtttta
    14641 ggaggatata tagatgaaac ttttgaaaaa aatgatgcaa gttctactag cagtcttttt
    14701 ctttggtttg ctagctacaa atacggtatt tgcgaatacc acaggtggcc gatttgttga
    14761 taaggataat agaaaatatt atgtaaaaga tgatcataaa gcaatctatt ggcataaaat
    14821 agacggtaaa acttactatt ttggtgatat tggagagatg gttgtcggtt ggcaatactt
    14881 agaaattcct ggaacaggtt atcgtgataa tttattcgat aaccaaccag ttaatgaaat
    14941 tggccttcag gagaagtggt actattttgg acaagatggt gctttgctag aacaaacaga
    15001 taaacaagta ctagaggcaa aaacgtctga aaatacagga aaagtatacg gtgaacaata
    15061 tcctctatct gctgaaaaga gaacttatta ttttgataat aattatgctg taaagacagg
    15121 ctggatttat gaagagggtc attggtatta tttaaataag ctaggaaatt ttggcgatga
    15181 ttcttacaat ccactaccaa ttggtgaagt tgctaagggt tggactcaag attttcatgt
    15241 tactattgac attgatagaa gcaaacctgc tccatggtac tacctagatg cttcaggtaa
    15301 gatgcttaca gattggcaaa aagtaaacgg aaaatggtat tattttggct cctctggttc
    15361 tatggcaaca ggttggaaat atgtacgagg caaatggtat tacttagata ataaaaatgg
    15421 tgatatgaaa acaggatggc aataccttgg taacaagtgg tactacctcc gttcatcagg
    15481 agctatggta actggctggt atcaagatgg ttcaacttgg tactatttag atccttctaa
    15541 tggagatatg aaaataggtt ggacaaaagt aaatggaaaa tggtattatc tcaattcaaa
    15601 tggagcaatg gttacaggta gccaaactat cgatggtaaa gtttataatt tcgcctcatc
    15661 tggtgagtgg atttaatatt ggagggtata tagatgaaaa ttttgaaaaa aactatgcaa
    15721 gttggactga cagtattttt ctttggtttg ctagggacca gtacagtatt tgcagatgat
    15781 tctgaaggat ggcagtttgt ccaagaaaac ggaagaacct actacaaaaa gggggacctc
    15841 aaagaaacct actggcgagt gattgatggt aagtactatt attttgattc tctatctgga
    15901 gagatggttg tcggctggca atatatcccg tttccatcta aaggtagtac aattggtcct
    15961 tacccaaatg gtatcagatt agaaggtttt ccaaagtcag agtggtacta cttcgataaa
    16021 aatggagtgc tacaagagtt tgttggttgg aaaacattag agattaaaac taaagacagt
    16081 gttggaagaa agtacgggga aaaacgtgaa gattcagaag ataaagaaga gaagcgttat
    16141 tatacgaact attactttaa tcaaaatcat tctttagaga caggttggct ttatgatcag
    16201 tctaactggt attatctagc taagacggaa attaatggag aaaactacct tggtggtgaa
    16261 agacgtgcgg ggtggataaa cgatgattcg acttggtact acctagatcc aacaactggt
    16321 attatgcaaa caggttggca atatctaggt aataagtggt actacctccg ttcctcagga
    16381 gcaatggcca ctggctggta tcaggaaggt accacttggt attatttaga ccacccaaat
    16441 ggcgatatga aaacaggttg gcaaaacctt gggaacaaat ggtactatct ccgttcatca
    16501 ggagctatgg caactggttg gtatcaagat ggttcaactt ggtactacct aaatgcaggt
    16561 aatggagaca tgaagacagg ttggttccag gtcaatggca actggtacta tgcttatagc
    16621 tcaggtgctt tggcagtgaa tacgaccgta gatggctatt ctgtcaacta taatggcgaa
    16681 tgggttcggt aatgacaggg cgcttataat tatattagaa cactcgctta atcaaagaag
    16741 tgactttgtt gtacttgacc tgacgattgg ggagattaaa agcatgtgaa atctacacat
    16801 gctttttcgt tagttatgag tgttgaactt gactaaccat tgacaaaaga taagagcaat
    16861 taaaataaga tacaaaaata tcaatgctaa ctgcaccgaa acgcccgatg ctatggatag
    16921 ggctaatagg gagagcaggg acggggcgag aatgtccact gtgctcagag cagagcccac
    16981 cgtccccatc ttgtcactgg ggatatgggc aaagacagct gcttgtaggc gagggctgag
    17041 aatgccgata atcgtagaac tgatgaaact ggtcatgaaa attacgatat aggcttgaca
    17101 aaggatacta gttattagag tcaataggga aatctcacag aaaaccaaga ggtgcgtgaa
    17161 acggatattt ttaaatagat tactgctggt catattgcca aggatagctc cacaagacat
    17221 gatgatggag agcagggcga ggagttgccc aatgctcagt tgtgctagtg tctgttggat
    17281 gaagcggagg gcaatcagcc gaggggacac atccaagatg gcaatcatac tggtcgtcag
    17341 aaacagtagt atgacggtat cttttaacct caggagcaca gccagcgact gtttaagatg
    17401 ttgaaaatat ttcttaaaac tcagtgctgt atgtgacatt tcaattcttt tttcaacctc
    17461 atacaaggta tgtcgaataa catacaggcc caaaaaggca atgacaaaag tcagagtgtt
    17521 gataagggaa atagtttgaa tacttataac attgataagg aatgcgccag ccagattggc
    17581 gacaatacgg acaaccctca tcagagactg cctgaacccc atggcatcat gcaggtcgtc
    17641 cttaattaca ctgatgtaga gggcgttcat catgtaggca gacaggtaac tcaaggtgtc
    17701 agagatgagg ttgatgatga caatcataat gactgaaact aatgaaaagg gcgacaagac
    17761 gagaaatact gtcaaaatag cgtagagcag gattttgatt ttggcaatcc agagtgcgtg
    17821 tttgactcta ttttcttgaa aatctcctag aatcccgaga aagagttgcg atagtagggg
    17881 aaccatctct gaaatggaaa tcagtaaaat cgctagcgga gcgaaattgt actgagccac
    17941 ataattgata atggcgaggt agaaaatact gtcgccgaat gatgaaaaac agctgtttag
    18001 cagcaactgt ctatatatgt tgtttgtcca aaataatttc atacttttct ccttatgtct
    18061 gcacatcgaa tgggaattta ttatttaata gacaccaata taccccatgc ttcctattcc
    18121 gaagtcaaac aaatctgctt taccactatc tccttgaaac ataagtaaac cgtgatcatc
    18181 tcgttcagaa catgctaaaa ttacctgttg gatttttttc atcagtaatt tgttttgatt
    18241 gtagacagtg gtttgtagta agctagttat tccgtggcaa tatcctgtca tcatttttag
    18301 atgatggtta aaaacaaatt
//
LOCUS       DAVLFB010000032        18210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLFB010000032 DAVLFB010000000
VERSION     DAVLFB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.032"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..1426)
                     /locus_tag="VXD58_001019"
     CDS             complement(<1..1426)
                     /locus_tag="VXD58_001019"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV0675737.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWCVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVKDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTA"
     gene            1532..3154
                     /locus_tag="VXD58_001020"
     CDS             1532..3154
                     /locus_tag="VXD58_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0675738.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            3358..5316
                     /locus_tag="VXD58_001021"
     CDS             3358..5316
                     /locus_tag="VXD58_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675739.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            5488..5721
                     /locus_tag="VXD58_001022"
     CDS             5488..5721
                     /locus_tag="VXD58_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV0675740.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            5727..7169
                     /locus_tag="VXD58_001023"
     CDS             5727..7169
                     /locus_tag="VXD58_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675741.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDMSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSFETPFALLFI
                     FATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTIYSSSKGSSVA
                     LENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            7327..8421
                     /locus_tag="VXD58_001024"
     CDS             7327..8421
                     /locus_tag="VXD58_001024"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0675742.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            8429..9595
                     /locus_tag="VXD58_001025"
     CDS             8429..9595
                     /locus_tag="VXD58_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0675743.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            9688..12786
                     /locus_tag="VXD58_001026"
     CDS             9688..12786
                     /locus_tag="VXD58_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV0675744.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLTQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            12834..13451
                     /locus_tag="VXD58_001027"
     CDS             12834..13451
                     /locus_tag="VXD58_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV0675745.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            13462..14796
                     /locus_tag="VXD58_001028"
     CDS             13462..14796
                     /locus_tag="VXD58_001028"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV0675746.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            14806..15270
                     /locus_tag="VXD58_001029"
     CDS             14806..15270
                     /locus_tag="VXD58_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675747.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            15267..15761
                     /locus_tag="VXD58_001030"
     CDS             15267..15761
                     /locus_tag="VXD58_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675748.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            15842..17497
                     /gene="mltG"
                     /locus_tag="VXD58_001031"
     CDS             15842..17497
                     /gene="mltG"
                     /locus_tag="VXD58_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV0675749.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            17568..18050
                     /gene="greA"
                     /locus_tag="VXD58_001032"
     CDS             17568..18050
                     /gene="greA"
                     /locus_tag="VXD58_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV0675750.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT         5446 a         3402 c         4068 g         5294 t
ORIGIN      
        1 cggctgtatc tcctgggtag gattctttct tgccctggat gatttggtaa caatcggctc
       61 ccttacccgc aataataact gcatctaatt cgtgatttgt gatagccatt gccgccttga
      121 tggcttcttg gcgatccgca atcttctcaa caggatgatt gatgtagcta ctaatttcat
      181 ctgcaatggc cattgggtct tcatagttag ggtcatcagc agtcagaaag acttgaatct
      241 cagggtgttg attgaggagg aggccaaagt ccttacgacg actttctccc ttgtttcctg
      301 ttgatcccag aaccagagca atctttccgg tttgatgagt ttcaaccaca ttgatgagtt
      361 ttttcagact atccccattg tgggcatagt cgatgaagac cttggctcca tttttctgag
      421 tgaggacttc catacgacca ggaacgcggg ttgcagcgat gccttttttg atgtcctcaa
      481 gacttgctcc gagacggaga caagcaagtc cagcagcaac tgcattttct tggttgaagt
      541 tgccaatgag ttggatatca taatctccag cgagtttacc cgtagctgaa aagctaaagg
      601 ctttggaatt ctcgatttgg ttatcaaatt ggctaccata gaaatcatgg tcttgatctt
      661 taacctgttc tttcaagact gagaagtggt ccatgtcact gttaatgatg actgctcggc
      721 tattttccat caagagacgc ttgtggtaga aatagtcttc aaagctaggg tgttcaatcg
      781 ggccgatatg gtctgggctg atattgagga aaactcccac atcaaaggtt agaccataga
      841 cacgtctgac cagataggat tgactggaga cttccatgat gaggtgggta cggtcatttt
      901 gcacagcctg attcatcatg tcaaagaggt caatactctc aggggttgtc aacgctgact
      961 taaagaaagt ctcgccatca agagttgtgt tcatggtcga caacatagca ggtcgatgcc
     1021 cttgagataa gatgttatag gcgaaatagg ctgctgttgt cttaccctta gtaccagtaa
     1081 aggcaaggag tttgagtttt tcctgtggat taccatagaa ctccatggca atcaaactca
     1141 tggctttctt tatatcgttc acaatgatga cagggatacc gacttcgtag tccttttcag
     1201 ctacacacca agctaagcct tgtgttatag cagaaagaag gtattctttt ttaaaggcag
     1261 cgccttttgc aaaaaaaaga gtgtcttctg ttacttttcg gctgtcgtag ctgatgctat
     1321 caaaaacaac tttgctgtag ttgtagtggt aatgaccttg gtcaataatt tcgcgaaaaa
     1381 ggccatcttt ctttaaaata tctaatacag tttcaatttt tatcatactt tctattgtaa
     1441 accgaaagtc gcaaatttac aagtaacaag gaaaagttta taatggaaga taaggagttt
     1501 ttcctagtta tcaaaattga atgaggaatc tatgtcgcac gaaaacaatc accagcaggc
     1561 ccagatgtta cgggggactg cttggctaac ggctagtaac tttatcagtc gcctactcgg
     1621 ggctgtttac attatccctt ggtacatctg gatgggggct tatgcagcta aggcaaatgg
     1681 tctctttacc atgggttaca atatctatgc ttggttcttg ttggtttcaa cagcggggat
     1741 tccagttgcg gtggccaagc aagttgccaa gtataatacc atgcgagaag aagagcatag
     1801 ctttgccctg attcggagct tcttaggctt tatgacagga ctaggcctgg tttttgcttt
     1861 agtcttgtat gtctttgctc cttggctagc agacttgtct ggcgtgggca aagacttgat
     1921 cccaatcatg caaagcttgg cttggggagt cttgattttc ccgtctatga gtgttatccg
     1981 aggatttttc caagggatga ataacctcaa accctatgcc atgagccaaa ttgctgagca
     2041 ggtcattcgt gttatctgga tgctcctagc aacctttatc attatgaagc tcggttcagg
     2101 agattatcta gcagccgtta cccaatcaac ctttgctgcc tttgtcggta tggtagccag
     2161 ttttgcagtc ttgatttatt tccttgccca agaaggttca ctcaaaagag tctttgaaac
     2221 aggagataag attaacagta agcgtctctt ggttgatacc attaaggaag ccattccttt
     2281 tatcctgaca gggtctgcca tccagctctt ccagattttg gatcagctga cctttatcaa
     2341 tagtatgagc tggtttacca actacagcaa tgaggacttg gttgtcatgt tttcttattt
     2401 ctcagccaat cctaataaaa tcacgatgat tttgatttct gtaggggttt cgattgggag
     2461 tgttggtttg ccacttttga cggaaaacta tgtcaagggg gacttgaaag cagcttctcg
     2521 tctggttcag gacagtctca ccctactctt tatgttcttg ctaccagcaa cggttggagt
     2581 ggttatggta ggagaacctc tatatacagt cttctatggt aagccagata gtttggctct
     2641 gggcttattt gtctttgcag ttttgcagtc tattatttta ggcttgtaca tggtcttgtc
     2701 tccaatgctt caggccatgt tccgcaaccg caaggccgtt ctctatttta tctatggttc
     2761 tattgccaag ctagtcttgc aactacctac catcgccctc ttccacagtt atggtccttt
     2821 gatttcaaca accattgctc tcatcattcc taacgtcttg atgtatcggg atatttgtaa
     2881 agtaactggt gtcaagcgca aggtgatttt gaagcgaacc attttaatca gtttgctgac
     2941 cctagtcatg tttctgttaa taggaaccat ccagtggctg ttaggatttt tcttccaacc
     3001 aagtggacgt ttgtggagct tcttttatgt agctcttgtc ggtgccatgg ggggtggact
     3061 ttatatggtt atgagtctgc gtacctattt attagataag gtaataggaa aagcccaagc
     3121 agatcgcctg cgagcaaaat ttaagctttc gtaaggtagc atttataaat aaaaggaata
     3181 agttgaacaa tcttttagga gaacttgaag cttattcctt ttttagcgta aaaatagcta
     3241 aaattatatt tcaaaaatag aaaatgaggc ttttttcgat tttttggaaa aaatcattga
     3301 ctaaacaaac taaacgttgt ataataaaac aaatatttaa agcaggaggt tccggaaatg
     3361 aaaaagtcta agagcaaata tctaaccttg gcaggtcttg tcctgggtac aggagtttta
     3421 ttgagcgcgt gtggaaattc tagcacggcg tcaaaaacat acaactatgt ttattcaagt
     3481 gatccatcta gcttgaacta tctagcagaa aaccgcgcag caacatccga tattgttgca
     3541 aatttggtag acgggttatt agaaaatgac caatatggga atattattcc atcattagca
     3601 gaggattgga ctgtttctca ggacggtttg acctatacct acaaacttcg taaggatgcc
     3661 aagtggttta cttctgaggg agaagaatat gcgcctgtaa ctgcccagga ttttgtgaca
     3721 ggtttgcaat atgcagctga taaaaaatca gaagccttgt atctagtgca ggactctgtt
     3781 gctggtttgg atgactatat cactggtaaa acaagcgact tttcaactgt cggtgtcaag
     3841 gcacttgatg accaaacggt tcaatatact ttggttaaac cagaacttta ctggaattca
     3901 aaaacacttg caacgatact ttttcctgtt aatgcagatt tcctgaaatc aaaaggggat
     3961 gattttggga aggcggatcc atctagtatt ttgtacaatg gacctttctt gatgaaagca
     4021 cttgtctcaa aatctgctat tgaatataag aaaaacccta attactggga tgctaagaat
     4081 gtctttgtag acgatgtgaa attgacctac tatgatggta gcgaccaaga atcactggaa
     4141 cgtaatttta cagctggtgc ttatactacg gctcgtcttt ttcctaacag ctccagctat
     4201 gaagggatta aagaaaaata caaaaacaat atcatctata gtatgcaaaa ttcaacttca
     4261 tatttcttta attttaacct agataggaag tcttacaatt atacttctaa aacaagtgac
     4321 attgaaaaga aatcgaccca ggaagcagtt ctcaataaaa acttccgtca ggctatcaat
     4381 tttgcttttg acagaacatc ttatggggct cagtctgaag ggaaagaagg tgcaacaaag
     4441 attttgcgta acctagtggt tcctccaaac tttgtcagta tcaagggaaa agactttggt
     4501 gaagttgtag cctctaagat ggtcaactat ggtaaggaat ggcaaggtat caactttgcg
     4561 gatggtcaag acccttacta caatcctgag aaagccaagg ctaagtttgc ggaagctaag
     4621 aaagaactcg aagcaaaggg tgttcaattc ccaatccact tggataagac tgtggaagta
     4681 acagataaag taggcataca aggagttagt tctatcaaac aatcaattga atctgtttta
     4741 ggttctgata atgtagtgat tgacattcag caattaacat cagatgagtt tgacagttca
     4801 ggctactttg ctcaaacagc tgctcagaaa gactatgatt tatatcatgg cggttgggga
     4861 cctgattatc aagacccgtc aacctatctc gatattttta atactaatag tggaggagtt
     4921 ctacaaaatc ttggactaga gcctggagaa gccaatgaca aggctaaggc agttggactg
     4981 gatgtctata ctcaaatgtt ggaagaagct aataaagagc aagatccagc caaacgttat
     5041 gagaaatatg ctgatattca agcttggttg attgatagtt ctttagttct tccaagtgtt
     5101 tcgcgtgggg gaacaccatc attgagaaga accgtaccat ttgctgctgc ctatggttta
     5161 accggtacaa aaggggttga atcatataaa tacctcaaag tacaagataa gattgtcaca
     5221 acagacgaat atgcaaaagc cagagaaaaa tggttgaaag aaaaagaaga atccaataaa
     5281 aaagcccaag aagaattggc aaaacatgtc aaataaaatc taattgtaga taagtttgtg
     5341 taagaagtct ttgtgcttaa aaacgcataa tatcaggtat ataaaatcct agatgttatg
     5401 cgttttgtta taggaagatt tattagattt tatcctcaaa ttgagttatt cccgacttta
     5461 tccgttgaaa tattataagg agtatttatg aaattaaaat tattaagagt agatactaag
     5521 gtgattatgg ggagtttctt acttgttctg tctagtctac ttgctttgtt gcttcccctt
     5581 atcttaaagg gtttaataga tgggagttct attgaaaata taggttccaa agtatttcaa
     5641 tcgtttttga tttttattgg tcaagccttg ttttcttcta ttggttacta tctgtttagt
     5701 caatcgggtg aaaaaagata gcaaaaatca ggaaaaaagt gatagagggg ttgatttatg
     5761 cagagaaatc cttctttgat aagagccaaa gtggggagtt gacttctgcc attgtcaatg
     5821 acatgagtgt cattcgtgag tttttaatta cgactttccc aaatattatt ctgagtttag
     5881 ttatggtact tggttccatt gtagtcttat ttagtcttga ttggaatctt tctctacttt
     5941 tattcatcac tcttccttgt atgatgttta ttatcttgcc cctttccaat atcagtgaaa
     6001 agtatagtcg tcgtttacag gaggaaatcg gatttttaac agggcaattg actgaaaaga
     6061 ttcaagaaca tgaattaatt aagactaacc aagcagagaa aagtgtacag gatgttttgg
     6121 ataattgtat tgaaagggta caaaacaatt cactgaaatc agatagggtt acttcttttg
     6181 agaccccgtt tgctctctta tttatctttg cgactatagt tgtgatgcta acctatgggg
     6241 gttatcgggt cagcgcagga tatatatctg tgggaacctt ggtttcgttt ttgatttacc
     6301 tctttcaatt acttaatcct attagtaata tagctaattt tgtaactatt tattctagta
     6361 gcaagggatc ttcagttgca ctggagaact tgcttgcagt tcctaaagaa aaatttgagg
     6421 gaggaaaatc ggtatcagga caagggttga attttaacca tgtctatttt ggttatgatg
     6481 aaaatcgacc tgtcttaaag gatattactt gttcaatttt caaggggcaa aaaattgctt
     6541 ttgttgggcc atctggatca ggaaaatcaa cgattgtgcg tttgttagag cggttttata
     6601 aaccgctttc aggagatatt ctaatggagc aatcaagtat atatgatttt aacttaaaag
     6661 aatggagaag taaaatcgct tgggtttcac aaaataatgc agtcttatct ggcagtattc
     6721 gtgacaatct ttgtctcggt ttgaatcgct tagtaactga tgatgaattg atgaaagtgc
     6781 tagacttagt atcactaggt gatgagattc gctccatgaa agagggacta gatactgaag
     6841 ttggtgaacg cggacgactc ttgtcagggg ggcgaagcca aagacttcaa atagctagag
     6901 cctacttaaa agatgctgaa attcttatat ttgatgaagc cactgctaat cttgatgcgg
     6961 attctgagta tgcgattatc agtagcctct attctgtact aaaggagaag acggttgtga
     7021 ttatagcgca tcgcttgtca acggtaaaag atgtggattg tattttcttc ttagaggagg
     7081 ggaaaatcac tggctcagga actcataagg aactactgga aaatcatgag cgttatgctc
     7141 gttttgtgca ggagcaaatg atagagtgaa ggtgtctttt gagattcacc attttatagt
     7201 ctattaaagg gagcaggaaa aactcccttt ttatatagtt tgaaactata actagctctt
     7261 gaaaagaaga aaatgagttg atgaaaataa gtggtacaat agttactata gatttggagg
     7321 aattgtatga gcaaggaatt acacattaac acaattttag cacaagcagg gatcaagtct
     7381 gatgaggcaa caggtgcttt ggtgacacca cttcattttt caacgaccta tcagcatcca
     7441 gagtttggtc gatctactgg gtttgactat acgcgcacta aaaatccaac tcgtagtaag
     7501 gctgaggaag tcttggcggc tattgagtca gcagactatg ccctagcgac tagctcaggg
     7561 atgtcagcta ttgtactggc ctttagcgtc tttccagtag gaagtaaggt cttggcagtg
     7621 cgtgatcttt acggtggttc ttttcgctgg tttaaccaag tggagcagga aggtcatttc
     7681 cattttacct atgccaatac agaagaagag ttgattgccg agttagaaaa ggatgtggat
     7741 gttctctata tcgaaacccc aaccaatccc ttgatgttgg aatttgatat cgaaaaacta
     7801 gcaaaattgg ctcatgctaa gggtgccaaa gtggtggtgg acaatacctt ctatagccct
     7861 atctaccaac gtccgattga agatggagca gatatcgttc tccattcagc aaccaagtat
     7921 ctagcaggcc acaatgatgt cttggctgga gtggttgtga ccaatagttt agaactatac
     7981 gagaagcttt tttacaatct taatacaaca ggggcagtct tgtctccatt tgacagctac
     8041 cagttgcttc gtggtctcaa gaccttgtct cttcgtatgg agcgctcaac agctaacgcc
     8101 caagaagtgg ttgccttttt gaaggattct ccagcagtta aggaagttct ctacactggt
     8161 cgtggaggca tgatttcctt taaagtagcc gatgaaacac gcattcctca tattttgaac
     8221 agtctcaagg tcttctcttt tgcggaaagt ttgggtggag tggaaagtct tattacttat
     8281 ccaacgactc aaactcatgc tgatattcca gcagaagtac gccattctta tggtttgaca
     8341 gatgacctct tgcgtttgtc tattgggatt gaggatgcta gagatttgat tgcagatttg
     8401 cgccaagcct tagaaggata agacaaagat gggaaaatat gattttacaa gcctgcccaa
     8461 ccgtttaggg caccatacct ataaatggaa agaaacagaa acggatagtg aagttctacc
     8521 agcttggata gcggatatgg actttgtagt cttgcctgaa atccaccaag ccgtgcaaac
     8581 ttacgcagac caactggttt atggttatac ctatgccagt gaagacttaa ttaaggaagt
     8641 tcaaaagtgg gaagctacac aatacggtta caactttgac aaagaggctc ttgtctttat
     8701 cgagggtgtg gtaccagcca tctcaacagc tattcaaacc tttacaaaag aaggcgaggc
     8761 ggttttaatt aacacgcctg tctacccacc ctttgctcgc agtgtcaagt tgaataatcg
     8821 tagattgatt actaattcct tagtggaaaa ggatagtctg tttgagattg actttgacca
     8881 acttgaaaag gatttggtgg aagaggaggt taaactctat gttctttgca accctcacaa
     8941 tcctggtgga cgtgtttggg aaaaagaagt gttggagaag attggccaac tctgccaaaa
     9001 acacggtgtt ttgttagttt cggatgagat tcaccaagat ttgaccctct ttggtcacaa
     9061 acaccagtct ttcaatacca tcaatcctgc cttcaaaaat tttgctatcg tcttgagcag
     9121 tgccactaaa acatttaata ttgctggaac aaaaaattcc tatgcagtca ttgaaaatcc
     9181 taagttgaga ctggctttcc agaaacgcct gttggccaat aatcagcatg aaatttcagg
     9241 cttgggttat ttggcgacag aagctgccta tcgatacggt aaagattggc tagaggaact
     9301 caagcaagtc tttgaagacc acatcaatta tgtggtggat ctatttggaa aagagactaa
     9361 aatcaaggtc atgaaaccgc aaggtaccta cttgatttgg cttgactttt cagcttatga
     9421 cctgactgat gaaacattgc aagagttgtt gagaaatgaa gccaaggtta tcctcaaccg
     9481 tggtttggat tttggagagg aaggaagtct ccatgcccgc atcaatatag ctatgcccaa
     9541 atctctgttg caagaagtct gtcagcggat tgtggctact tttgccaaat gttaaaaatc
     9601 cagccttcta ggagaaaagt cttcctagaa ggctattttc ataggcgaaa atatggtata
     9661 ataaaaagat aaggtaaagg tgaaaatatg gctaaattga ttccggggaa aattcgtatc
     9721 gaaggtgttg ccctttatga aactggtaag gttgatatca tcaaggaaaa gaacaatcgg
     9781 ctctacgctc gcgttgcaga agaagaactg cgctatagtt tagaggatga tttgattttt
     9841 tgtgcctgtg atttttttca aaagaggggc tactgtgtgc atttggcagc gctagagcat
     9901 tttctgaaaa atgatgagcg tggtcaggaa atcttgtgga gtctggaaga aggtcatgaa
     9961 gaaaaagagg ccgttgaaac caaggtgacc ttgggtggca agtttttgga tcgaatttta
    10021 tctccaaaat cagaatgcgc ctatgagtta tcagctgtgg gtcaggtgga agcaggaacc
    10081 aatcatatct tgtggactct gagaatcggc caaatcaata gccaaaaata ctatgtcatt
    10141 cgggatattc cacttttttt aaggattgtc gagcagagaa agtcgtatat gattggcaag
    10201 atttatgagg agtctctttc ttgggaagcc tttgatgatg ccagccaaga acttttgact
    10261 ttcttgcacg gactaacgga ggaagggctg gttccagacc tctttttcca aaaccaaggt
    10321 cggcacctct ttttccctct gacctttttt gagcagggtg tgaatttact gatggccttg
    10381 ccccattttc aatttgacca tcaagtggat agctaccaaa ccctgctttt tcaggatatg
    10441 catgctgatg ccaatctatt tgcctttaca gtaacagaat attcggacta ttttgaaatg
    10501 gaaatcagtg agagtcctag ggtcaatgtc ttttatcaag gagcagtact tttccataaa
    10561 ggtcaggttt attttttaac agaccagcag atgcgtcttc tcaaggaaat taaagcgtta
    10621 cctgtggatc gacaaggaaa gaaatacctg caatttgata gcagtgatcg agacaaattg
    10681 gcctcctgtc tgactctttt tatccagatg ggcaccgttt cagctccaga acgtctacaa
    10741 atcaaaactt ttgcgccctc tttttacttt gacagggaag aagataatcg gattcgttta
    10801 gaaattcagt ttgattatgg aaatagacag gtatctagcc gacaagagct agaagaatta
    10861 ccattttcaa gtgacgcgga cttagaagaa agaattttcc aagtctgttt ggctgcgggc
    10921 tttgaagccg attttcaatc ttggcgtcag gccttaaaag cggagtctgt ctatcatttt
    10981 tttcatgata tcattccagt ctttgaaaaa ctcgggcatg ttgacctatc agataaacta
    11041 gaagagcttt atagcctagc gagtcctcag gtgcagattg cctccaaggg aggtctcttg
    11101 gaaatccagt ttgattttca agatattgcc caggaggaaa tcgaccaagc catacaggcc
    11161 ttggttgcca atcaggattt ttatattgat tcgtctaatc aagtttactt tttcgatgaa
    11221 gaaaccaaga aaattcgcca aaatctacag gaactggggc aatttgaatt aaaagatggg
    11281 accttgcagg ctcggaaatc cttggcctac agtttagctc atctctttga agggcgagac
    11341 cgtgtttctt tttcacaaga attccagaat ctgacccagg atttgacgca tccagaggac
    11401 tttcctttgc aagcaactca ggtcaaggct gacttgcgag attatcagga aaagggaatt
    11461 ggctggttgc agatgctcca tcattatggt tttggtggga ttttggctga tgatatggga
    11521 cttgggaaaa cccttcagac cattgctttt ttgactagtc aagtgacaaa agaaagtcgg
    11581 gttttgattc tcgctccgtc gggtttgatt tacaactggg cagatgagtt tcagaaattt
    11641 gctccacagt tggatgtggc tgttgttcat ggtttgaaag caagtcgtga ggagattctt
    11701 gccgagagcc atcaaatcta tgtgactagc tatgccacct tccgtcagga cagtgagctt
    11761 tatcagggga tggcctttga cttccttttc ttagatgaag ctcaggtcat gaaaaatgcc
    11821 cagaccaaga ttgcccagac cttgagacaa tttgtggtgc cgtcggtctt tgccttgtca
    11881 ggaactccga ttgaaaacca tctgggtgag ttgtggtcta tttttcaaat cgtcatgccc
    11941 ggacttttgc caagcaagaa agaatttatg aaattgccag cagagcgagt ggctcagttt
    12001 atcaagcctt tcgtgatgcg acgcaagaaa gaagaagttc tgactgaatt gccagacttg
    12061 attgaggtgg tttataaaaa tgaactggaa gaccagcaaa aggctattta ccttgcccag
    12121 ttacaacaga tgcgagacca tctggctcaa gtgtcagaac aggaatttca gcgaagtcgt
    12181 gtggaaattt tatctggttt gatgcgcttg cgtcaaatat gtgacactcc tgccctgttt
    12241 atggaagatt atcagggagc cagcggcaaa ctagatagtc tccgagacct actggtacag
    12301 gtggcagacg gcggacaccg tgtcttgatt ttctctcagt tcaagggaat gttggaaaaa
    12361 attgaacaag aactgccaga cttgggcctg acatccttta aaattacggg ttcaacccca
    12421 gccaaggaaa gacaagacat gaccaaggcc tttaaccaag gagaaagaga tgcctttctg
    12481 atttccctca aggctggtgg ggtcggtctg aacctgacag gtgcagatac agtgatcttg
    12541 gttgaccttt ggtggaatcc tgcggtggaa gcgcaagcca ttggccgtgc ccatcggatg
    12601 ggtcaggaag aaacggttga ggtctatcgc ttggtgacca agggaaccat tgaagaaaaa
    12661 attcaggaac tccaagaaca aaagaaacat ctggtgtcac aagtattgga cggcacagag
    12721 tcacgtggta gtctgaccct agcagaaatt agagaaattt tgggaatttc tgaagccagc
    12781 acttgaaaaa tcaagacaat acgctataat gaagtgttga aaggaaatag aaaatgaaac
    12841 aagaacgatt tccattggtg tcagatgacg aggtcatgtt gactgaaatg ccagtcatga
    12901 atctctatga tgagtctgat ctgatcagta atatcaaggg tgagtatcga gataaaaatt
    12961 atttagaatg ggctcctatt gctgaagaaa aaccagtaaa accgattgaa aagcaagtag
    13021 ggaaacctaa aaaagctcct ttaggggtta aaaaagaagg aaagagctat gcggaggtgg
    13081 cgcgtgaaga agcgcgtgca gacttgaaaa agaaacgctc tgctaactat ctaactcagg
    13141 atttcagcct tgcgagacgt cattctcagc ccagtctagt tagacagggc aatcaaccga
    13201 caactccttt ccaaaaggaa aatcctggtg aatttgtcaa atatagccaa aaattgaccc
    13261 agtctcatta tatcttggcg gaagaagttc attctatccc taccaagaat gaagaagtgt
    13321 cagcacctgc tccaaagaaa aacaattatg attttctaaa gaagagccaa atctacaata
    13381 aaaaaagtaa acaaacagaa caagaacgtc gggttgccca agagttgaat ctgaccagaa
    13441 tgacagaata ggggagaaaa catgtcaaag acatatcatt ttatcggaat taagggatca
    13501 gggatgagtg ccttggcctt gatgttgtac cagatggggc acaaggttca gggatcagat
    13561 gttgaaaagt actactttac ccaacgcggt cttgagcagg caggaattac cattcttcct
    13621 tttgatgaaa agaatctaga cggtgatatg gaaattatcg ctggaaatgc ctttcgtcca
    13681 gataacaacg tcgaaattgc ctatgcggac caaaatggta tcagctacaa acgttaccat
    13741 gagtttctag gtagctttat gcgtgacttt gttagcatgg gagtagcagg agcacatgga
    13801 aaaacttcaa cgacaggtat gttgtctcat gtcttgtctc acattacaga taccagcttc
    13861 ttgattggag atgggacagg tcgtggttcg gccaatgcca aatattttgt ctttgaatct
    13921 gacgaatatg agcgtcactt catgccttac cacccagaat actctattat caccaacatt
    13981 gactttgacc atccagatta tttcacaagt ctcgaggatg ttttcaatgc ctttaacgac
    14041 tatgccaaac aaatcaccaa gggtcttttt gtctatggtg aagatgctga attgcgtaag
    14101 attacgtctg atgcaccaat ttattattat ggttttgaag ctgaaggcaa tgactttgta
    14161 gctagtgatc ttcttcgttc aacaactggt tcaaccttca ccgttcattt ccgtggacaa
    14221 aacttggggc aattccacat tccaaccttt ggtcgtcaca atatcatgaa tgcgacagcc
    14281 gttattggtc ttctttacac agcaggattt gatttgaact tggtgcgtga gcacttgaaa
    14341 acattttccg gtgttaaacg tcgtttcact gagaaaattg tcaatgatac agtgattatc
    14401 gatgactttg cccatcatcc aacagaaatt attgcgacct tggatgcggc tcgtcagaaa
    14461 tacccaagca aggaaattgt agcagtcttt caaccgcata cctttacaag aaccattgcc
    14521 ttgttggacg actttgccca tgctttaaac caagcagatg ctgtttatct agcgcaaatt
    14581 tatggctcgg ctcgtgaagt agatcatggt gacgttaagg tagaagacct agccaacaaa
    14641 atcaacaaaa aacaccaagt gattactgtt gaaaatgttt ctccactcct agaccatgac
    14701 aatgctgttt acgtctttat gggagcagga gacatccaaa cctatgaata ctcatttgag
    14761 cgtctcttgt ctaacttgac aagcaatgtt caataggatg ttcccatgga aattccaatt
    14821 aagatcattc aggcaagcaa gtctgatttg cctgagatag gggcacttca gacctcgtct
    14881 tttccagctg aaaagcagca actttcccat attttagaag aaagtatccg taagtgtgcg
    14941 gatacctttc ttctagctag ggatgaaaat caacttttag gctatatttt atcaagtccc
    15001 cagtcagaca atccgcaata tctaaaagta cattctttag tcatcgagtc tgaccatcag
    15061 agacagggct tgggaacact tcttcttgca gccttgaaag aggtggcagt tgagctggat
    15121 tacaaaggga ttcgtttgga gagtcctgat gagctgcttt cctattttga aatgaacggt
    15181 tttgttgatg aagaagcaac tttgctctat gcaactagcc agggctatag tatgatttgg
    15241 tttaatccct tttatctgga ggaacaatga aaatcagaca agcaagatta gaagatttgg
    15301 atcggattgt tgaacttgaa tttgaaaatt tttcggtcga agaagccatt cctccttctg
    15361 tctttgaagc acatttgaga gaaattcaga cctcttttct ggttgctgaa aaagaaggaa
    15421 gaatcatggg ttatatcgaa ggaccagttg gcctgcaccg ccatctgcaa gaccagtctt
    15481 ttacagaaga aataaaagac tatagtcatg agcctggtgg ttatatctct gtgacctgtc
    15541 tgtctattgc caaggaggca cagggactcg gactgggtca gaaattgctg acagccttga
    15601 aagaagttgc tcttgaagat gagagagatg gcattaatct aacctgtcat gactatctca
    15661 tcgcctacta tgaaaaacat ggatttgtca acgaaggcca gtcccagtca acctttgcag
    15721 gggaaacatg gtatgatatg gtctgggaaa tgaaaaaata agttaggaaa agtatcatag
    15781 attgcttttt ttggactttt aagatataat attatggatt atcaacaagg aggaaaaact
    15841 tttgagtgaa aagtcaagag aagaagagaa attaagcttt aaagagcaga ttctgagaga
    15901 tttagaaaaa gtaaaaggct atgatgaagt tctgaaagaa gatgaggcag tagttcgcac
    15961 tcctgcaaat gaaccttcaa ctgaagaact catggctgat tccttgtcaa cggtagagga
    16021 gattatgaga aaagctccta ccgtgcctac tcacccaagt caaggtgtac cagcttctcc
    16081 agcagatgag attcaaagag aaactcctgg tgttccaagt catccaagtc aagatgtacc
    16141 ttcttctcca gcggaagaaa gtggatcaag accaggtcca ggtcctgtta gacctaagaa
    16201 acttgaaaga gaatacaatg aaaccccaac aagggtagct gtttcctata cgacggcaga
    16261 gaaaaaagca gaacaagcag gtccagaaac acctacgcct gctacagaaa cagtggatat
    16321 catcagagat acatcacgtc gtagccgtag agaaggagca aaacccgtta agcctaagaa
    16381 agagaagaag tcacatgtga aagcttttgt gatttcattc cttgtattcc ttgccttgct
    16441 ctcagcaggt ggttactttg gttaccagta cgtgctagat tccttattac ctatcgatgc
    16501 taattctaag aaatatgtga cggttggaat tccagaaggt tcaaacgttc aagaaatcgg
    16561 tacgacgctt gaaaaagctg gtttggtaaa gcatggtctg atttttagtt tttatgccaa
    16621 gtataaaaat tataccgact tgaaagcagg ttactacaat ttgcaaaaga gtatgagtac
    16681 agaagactta ctcaaagagt tgcaaaaagg tggaacagat gaaccgcaag aacctgtact
    16741 tgcgactttg acaattccag aaggttatac cttggatcag attgctcaag ctgtgggtca
    16801 attgcaaggt gacttcaaag agtctttgac agcggagact ttcttggcta aagttcaaga
    16861 tgagacgttt atcagtcaag cagtagcgaa atatcctact ttactggaaa gtttgcctgt
    16921 aaaagacagc ggtgcgcgtt atcgtttgga aggatacctt ttcccagcta catactctat
    16981 caaggaaagc acaactattg agagcttgat tgatgagatg ttagctgcta tggataagaa
    17041 cctatctcct tactatagta ctatcaaatc taaaaacttg actgtcaatg agttgttgac
    17101 cattgcttcc ttggtcgaaa aagaaggtgc caagacagaa gatcgtaagc tcattgcagg
    17161 tgtattctac aatcgtttga atcgtgatat gccacttcaa agtaatattg caatcttgta
    17221 tgcccaagga aaactggggc aaaatatcag tctagctgag gatgttgcga ttgataccaa
    17281 cattgattca ccttataatg tttataaaaa tgtaggtctc atgcctggtc cagtcgatag
    17341 tccaagtctg gatgcgattg agtcaagcat caatcaaact aagagcgata acctctactt
    17401 tgtagcagat gtcacagaag gcaaggtcta ctatgctaac aatcaagaag accacgaccg
    17461 caatgtcgct gaacatgtca acagcaaatt aaactaaaca aactaaaatt atgttatact
    17521 tcacataatt ttctttagaa aatattatca gaagaaagtt gagaaaaatg gcagaaaaaa
    17581 catatcctat gacccttgag gaaaaggaaa aacttgaaaa agaattagaa gaattgaaat
    17641 tggttcgtcg accagaagtg gtagaacgca ttaagattgc ccgttcatac ggtgaccttt
    17701 cagaaaacag tgagtacgaa gcagctaagg atgaacaagc ctttgtcgaa ggacaaatct
    17761 ctagcttaga aacaaaaatc cgctatgctg aaatcgtcaa tagcgacgca gttgcccagg
    17821 acgaagtagc gattggtaaa acagtcacca tccaagaaat tggtgaggac gaagaagaag
    17881 tttatattat cgtaggttca gctggtgcgg atgcctttgc aggtaaggtt tcaaatgaaa
    17941 gcccaattgg gcaggccttg attggcaaga aaacaggtga tacagcaacc attgaaacgc
    18001 ctgttggtag ctatgatgta aaaatcttga aggttgaaaa aacagcctaa aaacagaaaa
    18061 aggagtgggg aggcgatgtg cttcactcac tcctttttcc attttgctat tcttcgaaaa
    18121 tctcttcaaa ccacgtcagc gtcgccttgc cgtatgtatg gttactgact tcgtcagttt
    18181 catctacaac ctcaaaacca tgttttgagc
//
LOCUS       DAVLFB010000033        17509 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLFB010000033 DAVLFB010000000
VERSION     DAVLFB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17509
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.033"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(565..1701)
                     /locus_tag="VXD58_001033"
     CDS             complement(565..1701)
                     /locus_tag="VXD58_001033"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV0675751.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(1763..2200)
                     /locus_tag="VXD58_001034"
     CDS             complement(1763..2200)
                     /locus_tag="VXD58_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0675752.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(2202..4157)
                     /locus_tag="VXD58_001035"
     CDS             complement(2202..4157)
                     /locus_tag="VXD58_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0675753.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEIMKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(4181..5950)
                     /locus_tag="VXD58_001036"
     CDS             complement(4181..5950)
                     /locus_tag="VXD58_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV0675754.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            6159..7499
                     /locus_tag="VXD58_001037"
                     /pseudo
     CDS             6159..7499
                     /locus_tag="VXD58_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7627..8649)
                     /gene="cbpF"
                     /locus_tag="VXD58_001038"
     CDS             complement(7627..8649)
                     /gene="cbpF"
                     /locus_tag="VXD58_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEV0675755.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRQEIAFRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWV
                     YDDGDWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQHLGNKWYYLRSSGAMATGWYQDGSTWYYLDAETGDMKTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(8668..9489)
                     /gene="cbpG"
                     /locus_tag="VXD58_001039"
     CDS             complement(8668..9489)
                     /gene="cbpG"
                     /locus_tag="VXD58_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV0675756.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHSYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(9570..9809)
                     /locus_tag="VXD58_001040"
     CDS             complement(9570..9809)
                     /locus_tag="VXD58_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675757.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(9959..10225)
                     /locus_tag="VXD58_001041"
                     /pseudo
     CDS             complement(9959..10225)
                     /locus_tag="VXD58_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10250..10561)
                     /locus_tag="VXD58_001042"
     CDS             complement(10250..10561)
                     /locus_tag="VXD58_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV0675758.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(10486..10717)
                     /locus_tag="VXD58_001043"
                     /pseudo
     CDS             complement(10486..10717)
                     /locus_tag="VXD58_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10718..11350)
                     /locus_tag="VXD58_001044"
     CDS             complement(10718..11350)
                     /locus_tag="VXD58_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675759.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(11364..12359)
                     /locus_tag="VXD58_001045"
     CDS             complement(11364..12359)
                     /locus_tag="VXD58_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0675760.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(12356..13054)
                     /locus_tag="VXD58_001046"
     CDS             complement(12356..13054)
                     /locus_tag="VXD58_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV0675761.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(13131..14141)
                     /locus_tag="VXD58_001047"
     CDS             complement(13131..14141)
                     /locus_tag="VXD58_001047"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV0675762.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(14125..15132)
                     /locus_tag="VXD58_001048"
     CDS             complement(14125..15132)
                     /locus_tag="VXD58_001048"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV0675763.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDILIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSFEEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(15119..16072)
                     /gene="mvaD"
                     /locus_tag="VXD58_001049"
     CDS             complement(15119..16072)
                     /gene="mvaD"
                     /locus_tag="VXD58_001049"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV0675764.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEYLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(16054..16932)
                     /gene="mvk"
                     /locus_tag="VXD58_001050"
     CDS             complement(16054..16932)
                     /gene="mvk"
                     /locus_tag="VXD58_001050"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV0675765.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITETCVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(17054..17233)
                     /locus_tag="VXD58_001051"
     CDS             complement(17054..17233)
                     /locus_tag="VXD58_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV0675766.1"
                     /translation="MLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNT
                     TVDGYFVNYNGEWVQ"
BASE COUNT         5382 a         3840 c         3064 g         5223 t
ORIGIN      
        1 aaacattttt agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tgtttcattt
       61 tactatatag atcacattcg aaagaattcg ttccaaaatt gcttggttcg ttcttctctt
      121 ggacgactaa agaattgatg ggcattattt ttttcaacaa ttttcccact gtccataaaa
      181 ataacatgat gggctacatc tctagcaaaa cctatctcat aaagctgatc aaaaactcaa
      241 tctgaggaga aaatccaata agactttcca taataaagga catcctatca agatttttga
      301 acacccgata ggctgccttt ttccgatttt gaagactttt tgtccagctt cttttacctt
      361 ataaataaca acaaaaatca aagacaagct agaaagatta ccgcagataa ctcaaacagt
      421 gttttgaggt tgcagataag actgacggag tcagctcaaa gcactgcttt gaggttgtag
      481 atagaactga cgaagtcagc tcaaaatact gttttgaggt tgcagatgga tgctaacctg
      541 atttgaagag attttcgaaa aagattaaat atactctaca atttgctcaa tcaattcttg
      601 gtcgtctaaa cctgtaactt gtataacaac atctttgact gattgtttag ccaacaattc
      661 acctaatcga atactttctt catcatttac atcgcgatag tcaaaggcat agccaactgt
      721 tttaagtagg tttttatatg acaaactgag ttctttcaat tcacgtatcg gccggatgaa
      781 tcgttcatta tagcctaatt ttcggattgg agtacgagct acgcgactaa cctcgtccac
      841 tatgaaaggg ttttcgaatc gttctataat gactttgtga taattctcca attctttttt
      901 atcaaagttc catttggcaa tcaagagact ccgaatttca gctaatacag attcaatccg
      961 agatttgata ttaggatttt gaagagcttc caaaattgtc ttggcaccat aatgcgcacc
     1021 aatgtaagct gaagttgcat gtccagaatt gactgaaaaa agttttcgct caataaaggg
     1081 ttctaaatct tcttcataat gcacatcttc tagacgtaaa tctggatttt taagacgctt
     1141 ggtttccacg acccattcat taaagggctc gaccataaca aaaagggaat cttcgtgact
     1201 ttgtgttgga acaatcctgt ctactgcagc atttggaaaa cctatgtagt tatcagcaaa
     1261 tgtcaaacct tccggactta aatatttctt gacttcttga taaagaaatt gagacccgcc
     1321 aatcatattt tcacaggcca taacatccaa tgcctgtgta tttcctgcaa ctcggcgagc
     1381 ttcgattcct ttggctagaa gttcagcgat aaaagggagt atattaggtc cgattgcagt
     1441 agtaataata tccgtctttt gaatcgcttc aatgacttgc tcaggatgtt ctttgctatt
     1501 aattccagcc acattagtta cttctatacg agactgtcct ttctgtgcaa tttcaatttc
     1561 atacttgccc ttttcattta gagcatgaat tatctgatta ttgacatcca caaaatcaat
     1621 atggaaatca tttttaaata gaatttcacc tataaaacca cgaccgatat taccggcacc
     1681 aaaatgaaca gaatgtttca ttcaatttct ccttttccta tttgagggac atatatacta
     1741 gatgacaaat gacgaaattc aattattcaa cagcatcaaa taagcgcaat acttcctctt
     1801 ttgactgagc atctgctagt ttaagaacat tatctacatc tgcacagaag atagaaattt
     1861 tctgaataat ttctaagtgt tcattaccaa taccagcaat accaaaaaga accgttgcca
     1921 cttgagggtt agatacattc ccaaaatcaa cccctctagg gacttgaacg actgtaatac
     1981 cagacttgag gacatcattt tttgctgcat ctgttccatg cggtatggcg ataaagttac
     2041 ccatgtaaac agatagctct ttatctcgct caatcatggc ttcaatatag tcttcattaa
     2101 catatccacc ctcataaaga acttgcccac aataacaaat agcttcctcc ttgttagaaa
     2161 attgtttatt aagcttaatc aaatgttttt caagtttcat attagttctc caatttctta
     2221 attttttcgt taaaaatagt atttagcatc tgataaataa tggattgatt tcccgtcttg
     2281 taaatctctg tataaagatg attttcaata atcgactgac tgatagctgt cattaaatct
     2341 ctcgcttcct cgctagcttc tttagacatt agcattagca gacacctttt gacctcttct
     2401 tcctctcgtt tcattgacaa tgcggagata ggtagtttga gttcaaacat agtaaaacta
     2461 tttgttgtta ctttgctaga ctgactatgt aaaagcacca gaccagtatt aggaatagcc
     2521 ataggactat tctggaagtg agataacaat tttcttgtta ggtaatctct gtcactcaca
     2581 ttcttctgat attggatgat ttgttctacc gtgtgctcaa atgattgatt attctccaat
     2641 tgaaccaact cgaattgact caaaagctga ctactagata ttaaataatc ttgcaagtca
     2701 taacttttct ctggagcgat agcatcacgc gcgacaattg tacgatgtgc ctgtacatac
     2761 tgaagtcgtt cctgtaattc aagcaattct ttgacggttg gaaaactaga aattacatcg
     2821 atttcctgat tagtcagcgg cttggtagat aaaatataat catactgact gagatcaata
     2881 ctgtggtaat ctactagaga ctgaatatct atcaactcta caaaaggagc aacactctta
     2941 attttactga ctaataaatc tctagtgacc cgacgttcat ccgttaaaag caaaacacgg
     3001 actggataaa tctctggact acggcctaag ctagagataa aatgtagggc aatcatgcca
     3061 tactcatact ctgtatgaag atatttcgga aaaatatcat tcactaaaag actgatgact
     3121 gtataaagaa atttattcct ttcaactaaa atctggacag attctggcaa attttcaccc
     3181 tgaaaaagga tagggacgcc taaactgagc cgaatatgat ggaaaagaaa attgaataaa
     3241 accttgtcct taaaaaagtc aatcttggta tacatggaaa ccgtatcaat cagatttgaa
     3301 atattgtaga aaaattcacc atcaaatttc tccgtaaaga gcggattgtc ctgacgttta
     3361 atgattaatt catccaagat gctcgcaaaa tagataattt cctgaatact ataaaattgt
     3421 gcagtctgct tagagtaagt ttgaaaaatt ttttgagaaa tttccaaagc ctgcttacta
     3481 gtattaggtg aattttcaat gttttgctcc tgacctataa gagataacaa gatcgcaaaa
     3541 aacatcttca tcctagcatc catatctgga aaacctgaca gttgcttatt aacaatctga
     3601 ctggccagcc tagttctatc tgcttctaaa atatcaaagc tcccaaaatt accggttgaa
     3661 aaatctgcta ctgagataca gtttgtcagt aaaatagcca aaaatcttct cttaccaact
     3721 gaatcaccag aaatccgata acctttttgt cgttcaattt tcagatcaaa gtctaaaaga
     3781 cgcttatcaa tatctgaaat atcctgaata atagttacat tactgacttt cgtgcactct
     3841 tgcaatgctt cattggtgac aaaaccgctc tcagtcagaa ggcgataggt aatcaactct
     3901 tgtctttctt ggggactata ctcaactaac acttcaagtt ctgtcggcaa atcatccaac
     3961 tctccagtca aaatatagta tttcccatgc ttcattattt cgattccata ttgctccatg
     4021 ctatctgtca aatctgataa agttcgataa attgttctag atgaaacctg taagatttca
     4081 gtcatatctt gcattgaaag cttccctaca tgtaggaaag ccttcaataa ttgttcttct
     4141 cgtttggtta atagcattcg taatttagaa acctctagtt ctatttgtac attctagcag
     4201 ccattttgtc atattctggt gttgttacta aactatccac aataagaaat tgagaattcg
     4261 gtgctgcttg ctgcacttct gcctgtaaag aaatagttgt tacaatcatt atgtttttag
     4321 aattaaattc acctaattct gaaattttgg cagtagaaac tggaatacga atattcttgt
     4381 ttctaaagat agcccgaatc gtttcacagc ccatagttgc agttccctgt gctttaccat
     4441 aagcaactac tacagcatca atatggttaa ggtcaatttc ctgactatct actggtgctg
     4501 aagttggtat atctccttca atttctgcta ttggagaagc tcctgttaat gaagctacaa
     4561 tttcatcata acgaggggac gctaagaaat tatcaacaga aacatgaata gcacttggac
     4621 tcttgtcttt agctcttggt gtcagttctt cctgagtaac aattaatgtt tttggtgtat
     4681 caagcaaatt tgagattgcc tgattagata ctggaatctc tagacctgct tttttaacct
     4741 tatctcgaag aatactagct cccatagcag agcttcccat accagcatcg caggcgaaaa
     4801 tgattttttc cactgagtct gtcgaaacaa ctgcatcaac agaagttgat actaactgac
     4861 ctttagactg agccttagct gcttgggtag ccgcctgagc agcttcgagc gaatcctcag
     4921 ttgacttgtc tgcatgaaga ataagggctg ctacaaggaa agaaacaact gctgctacta
     4981 aaacacctag aagaacattt agatggggcc aaacaccttt tggcgccgta gccataatcg
     5041 caataataga acctggtgaa gctggagatt taagaccagc gtctaagagt tgaaaagtaa
     5101 aagttccaga gatacctcct gccatagcag ctaaaaatag agtaggcttc atcataacat
     5161 aaggaaagta aatttcatga atccctccga agaaatgaat aaccattgcc ccccaagaag
     5221 aagatttagc agaaccttta ccgaatacag cataagctaa tagaattccc aggcctggtc
     5281 caggattagc ttccaatagg aagagaattg acttaccagc ttgagctacc tgttctactc
     5341 ccagaggagt aaaaatgcca tgattgaggg cattattgag gaaaaggact ttagccggtt
     5401 cgatgataat attagccata ggaaggaggc gagcattgac aatagcctca acaccattcc
     5461 caacagctcc agtaagagtc gatacgactg gaccgattgc gtagaaagcc aaaagcaata
     5521 atgcaaaacc aacgagacca gctgagaagt tattaactaa catttcaaat ccgggacgaa
     5581 ttttttcttg gaacttctca tcaaatttct tgatagtcca tcctcccagt gggcccatta
     5641 ccatagctcc gataaacata ggaacactag aacctgtgat tgcaccaaca gtagcaatag
     5701 ctcctacaac ggcaccacgt tggccatgga tcatatatcc acctgtgtaa ccaatcagga
     5761 ttggcaataa atacgttaac ataggaccaa caacagtagc taactgttca tttggcagat
     5821 agccatcagc gataaagagg gcagtcaata ctccccaagc aataaatgct ccaatattgg
     5881 gcataaccat atttgaaagc gatgtcccta gtttttgaac cctgactttc aatgatactt
     5941 tttcttccat tagatcatcc tccttttctt aaaactagta tatacaaagt taaaaacctt
     6001 taaaactaaa gttttgtcac agattttgtg tcaaaacatt acttttgacg aggctgggca
     6061 aaaaggctta aaaattataa aacgcatcat atcaggtgta catgaactac accccaaaag
     6121 ttagacagaa aaaatctaac ttttggggtg tttttattat gaaactaact tatgatgata
     6181 aagttcagat ctatgaactt agaaaacaag gatatagctt agagaagctt tcaaataaat
     6241 ttgggataag caattctaat attaggtaca tgattaaatt gattgatcgt tacggaatag
     6301 agttcgtcaa aaaaggaaaa aatcgttact attctcctga tttaaaacaa gaaatgagta
     6361 ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac ggtctcccaa
     6421 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
     6481 agaaaacaag agggagagta cctaaaatgg gacgtaagcc aaaaacgaga cctgaagaga
     6541 ggacagaatt agaacgtctt caagcagaaa atgagtacct gagagcggag aatgccatcc
     6601 taaaaaagtt aagagaactc cgattgaagg aggagaaaga agaaagacag aaattgttca
     6661 agaattaatg actgaatttt cgttagctct tcttctaaaa gtcattaaac tagctcgttc
     6721 gacctactac tatcacttga aacagctaga taaaccagat aaggactaag agcttaaagc
     6781 tgaaattcaa tccattttta tcgaacacaa gggaaattat acttatcgcc gggttcattt
     6841 agaactaaga aatcgtggtt atctggtaaa tcataaaaga attcaaggct tgatgaaagt
     6901 actcaattta caagctaaaa cgcgacagaa acgaaaatat tcttctcata aaagacgttg
     6961 gcaagaaggc agagaatctc attcaacgcc aatttgaagg ctctaaaaca atggaaaagt
     7021 gctacacaga tgtgacagaa tttgccattc cagcaagtac tcaaaagctt tacttatcac
     7081 cagttttaga tggctttaat agcgaaatta tcgcctataa tctttcaact tcacccaact
     7141 tagaacaagt acaaacaatg ttggaacagg cattcacaga gaagcactac gagaatacga
     7201 ttctccatag tgaccaaggc tggcaatacc aacacgattc ttatcatcgg ttcctagaga
     7261 gtaagggaat tcaagcatcc atgtcacgta agggaaacag cccagacaat agtatgatgg
     7321 agtccttctt tgggattctg aaatcggaaa tgttttacgg ttatgagaag tcgtttcagt
     7381 cgcttaagca attggaacaa gccattatag actatattga ttactacaac aataaacgaa
     7441 ttaaggtaaa actaaaagga cttagccctg tgcaatacag aactaaatcc ttcggataaa
     7501 ttaattgtct aactgtttga gggcagtaca gaaaaacctt gatactatgc gttttttgat
     7561 tgtgaagact tttatccacc tattatacaa agttaagtat ccatcacagt ttacaattag
     7621 ccttcattac ttaacccatt caccattata gtttaagtag taaccaccta ctgtggtatt
     7681 aacagctaaa gcacctgaat cataggcata gtaccagtta ccattgactt ggaaccagcc
     7741 tgttttcata tctccattac ttgcatttag atagtaccaa gtcgaacctt cctgatacca
     7801 accagttgcc atagctcctg atgaacggag atagtaccat ttgttcccaa ggttttgcca
     7861 accagttttc atatcgccag tttcagcatc taaatagtac caagttgaac catcttgata
     7921 ccagccagtt gccatagctc ctgatgaacg gagatagtac catttattac caagatgttg
     7981 ccaacctgtt tgcatgatgc cagttgctgg atctagatag taccatggag ctgcttttag
     8041 cttctcttca tcataggtaa gagggtaatc cttaacccaa ccacgtgcta gctctccaac
     8101 cgtcaatctg ttgatgcgag aatcaaagcc accatccttc tgcaaataat accagtcccc
     8161 atcatcataa acccaaccag tttttaaagt atgataacta cgctgatctt caaaataata
     8221 gactcgtttc tctgctgggc tatcatattc ttccccatga tgtttgttgg tatttgtagc
     8281 agtttttgct tctaacactt gcttgccaac aaattcttgt agtaccccat cttgaccaaa
     8341 gtaaaaccaa tctggtctaa aagcaatctc ttgtcttgga gaaggaccaa tcgtaacccc
     8401 cttgtgtgga gcaggtatat attgccagcc gacaaccatc tctccggata aaggatcaaa
     8461 ataatagtat ttcccatcta tcactctcca gtaggtttct tttagatccc cctttttgta
     8521 gtaggttcta ccattttctt ggacaaactg ccatccttca gaatcatctg caaatactgt
     8581 actggtccct agcaaaccga agaaaaatgt ggctagtgcg acttgcatca tttttttcaa
     8641 aagcttcatt ttatatatcc tccaacatta aatccactca ccagatgaag cgaagttata
     8701 aactttacca tcgatagttt ggctacctgt aaccattgct ccatttgaat tgagataata
     8761 ccattttcca tttacttttt gccaatctgt aagcatctta ccggaactgt ctaaataata
     8821 ccaagtatca tttatctcct gccaacccgt ttgtttatca tgttgtctat aatggtacca
     8881 actaccattt attttcttcc atccttcaag agagtaacct ttaagaaccg aataaataaa
     8941 tggcaaattt cgttcattta atttaactgc actgttaatt tgattagctc catctcctaa
     9001 agtatgcact cctactacac ggtgactagc atcataaact gtagatccac tagacccctc
     9061 taaagtatca acttggtaat ccaagaacat gccatcatca cttaaaactt gttttttatc
     9121 tgtatacatt tgatgaactt taaaattata tgatggatag cctgtgatag tcacagttat
     9181 tcctgtcaaa tttttttgac tagtaggaag acccaaagtc cctaattttg caccaatggg
     9241 ctcttctaga attaataaag ccaagtcata ttcccttgca tccttagaat ttaaatttct
     9301 aaattccttc aaataacgaa cttcctttac tttgatcttt ccaaatggtt cttgacttgg
     9361 actaacagcc ggaagaacat aaatatcatc cgcttctttc ccatagtcat gtctgtagta
     9421 gctgtgagcc gctgtaatta acacattatc tttaataaaa ttcgcacttc ccctcccttc
     9481 tacattcatt ccatcggcta ctccataata cttagataaa accatagctg aagtcgaata
     9541 cggatactgt aaagtattat caattttaat caaatcatca ttaccgataa tacttctgat
     9601 tgcttttggt agtatgaacc atatgttggt gaaatctcag ataatgaaga atcattagat
     9661 cctggacctt tttctagtgt ctcacttacc tcatattctt cacccgtact agaaataaca
     9721 ctcaaagcag atactgtcga taactggcta gccaataaag tactcgcaat aattgaaata
     9781 cccaattttt tataaacagt tttcttcatt attgtatcct cctaatgtaa ttatagcgta
     9841 ctattctaaa tttcttaatc tactatagaa tcaagaaatc taccaccttc tttaaatacc
     9901 ctccattatc acataaacag gtaaactttt caattaatga ctgcgctttt caatcacgct
     9961 agaggtactt acttgcttct ttgatactaa gttcagccat tctttccttg tttttctcaa
    10021 taaagcatgt tacccaagtg ggattcgttt tggagtagtc tcgcagagtc cagccaatgg
    10081 ctttattgat aaaaaattct gtttggttca agttatgaag gagaatcttt tccattaatt
    10141 gagtattggt cttctctttt cttaacaact ggttgtcaat agcgacacgt ctcagccaga
    10201 tattatctga taggctccat tttatactca atgaaaatca aagagcaaac taggaagcta
    10261 gccgcagatt gctcaaaaca ctgttttgag gttgcagata gagctgacgt ggtttgaaga
    10321 gattttcgaa gaatattaag attatttctt ctagttcagg atgtttatac accaaactcc
    10381 ctactactcg atctaggata tctaccgtgt cccaccagga ttttgtcacg actaactgct
    10441 ctagcttagg caaatcggtt tcctttagat aagactgcat tgctttcaaa tagttagcag
    10501 ccacatattg gtattttcta ggctcctttt cccagcaagt gtctgcaaaa tcccaatcga
    10561 taatctttgt ttttttcgct tctggaaaat atttttatag agtttatttc tttcaaccta
    10621 gaaaagaaaa ttgatggcac atataggctt ccatgggcct tgctttttta gagtcttttg
    10681 ctgcttctag ctcctcaagt aaatctgcta aactcatcta aaactcctca tgccccacca
    10741 aatggtgctg aaaggcatag acagccgcct gggtacgatc gctaacttca agtttggcaa
    10801 gaatgttgga cacgtgggtc ttgaccgtct tgagagagat aaagaggtca tctgcgatgc
    10861 gctgattttc gtagcccttg gcgatgagtt ggagaacatc tcgctcacgc gcagtcaatt
    10921 cttcatgaag ttccatatga ttgcggtggt attcaacctt cttgctaacc tcttgctcaa
    10981 tggccagctc gccagcagct accttactga cggcatgaag caattcatct gcactagaag
    11041 tcttgagcat atagcctttg gcaccagcat ctaagactgg catgattttt tcattgtcca
    11101 aataagaggt cacaatcaaa atcttggctt caggccattc tttaaggatt gctaaggtcg
    11161 cgtcaatccc attcatctca ggcatgacaa tatccatgac aatgacatct ggacgcagtt
    11221 ccaaggccaa gtcaatccct tgagacccgt tggacgcctc acccacaact tctacatcgt
    11281 cttggaggtc aaagtagctt ttcaagccca atcggaccat ttcatggtca tctactagta
    11341 aaattttcat ctttactcct ttatcattcc ttatctaaca ggggaatacg gatatcaacc
    11401 gccagccctt gcttgggagc tgtcaagagt tgaactgttc cagccatatc ttcaacccgc
    11461 tccttgatat ttcgcagtcc ataactcaag tcgtctaagc tccctaactg gaaaccaatc
    11521 ccattgtcca ccaccttcag ttgcaattca acatctgtct gatagaggta gacatctagg
    11581 caagatgcct gggcatggcg gagggtattg ctaatcaact cttgcaggat acggaagata
    11641 tgctcctcga ttttcttagg caatttcgtc atattctgct tgagactaac cctaagatca
    11701 ctcttgtcct caagctcttt taagagaatc tgaatccctt ctatcaagct cttctgctcc
    11761 agttcaactg gtcgcaaatg caagagcaaa acccgcaaat ccttctgggc tgtttctaaa
    11821 atagctgtga cactctgcaa ctggatctgc atcttttctc tatccaattt caaagcctgc
    11881 tgactgatac ccgataaaat catgtgggcc gcaaacaact cctgactgac tgtatcgtgc
    11941 aaatcccgag caattcgctt ccgttccttc tcgatgattt cctcttcctg agcaaggctc
    12001 tgattttcag ctttttgaag agcttctgtc aaaaggttaa gtttacctga taaggacttg
    12061 aaactggcat ccaaatctgg atctgcaacc tgaaccactt cttgccctgc caataaacgc
    12121 ttgagattag cctgcatttt tcttagagaa agctcttcga tccctcgcca aaacagggct
    12181 aagagacagg ttatggacat gctgaaaacc aacaataaaa agacaaattt ttctgttttt
    12241 tcgacatcgt gcaaaaagat agaccagtca aaatcaagta tttccagcaa gctgtgggag
    12301 aaaaaaaaga caaacaggaa ggaggtgaga gcaataatga cataggcttg ttttttcatc
    12361 ctctaaccac ctccacatca ccaatcatag tggtcaagaa aatcttgaca ctcttgttac
    12421 tcttgagata gtcttttgtt tcttgatgat agtgttcatt gcggagggct cgcttgggct
    12481 ggttgaaaaa agtcaaatcc ccatagagac agttaacgct gagactgact tccacatcta
    12541 caggtacgat gattttggtc gttcctacca tctttctgag gataatgaca ttgtcatgat
    12601 tggttaagat gaccctctcc agatgaatag tgtccttgcc catgaagcga aagagattga
    12661 tatcatcgaa ttggcaagtc tggtagcttg aaaaatgatg aagatttcca aaccaacgat
    12721 ttttctcctt cttaaccgtc acgacctctt caaaaaccaa attggtctgc tctttttcct
    12781 ggttcatcat cgggtaaaga agaaagaggc tatagataac cgcaacaaaa atagctagaa
    12841 tcacaaaagg attgagcata acgatgaaaa agaagagaat ggttgccgct actaaaagaa
    12901 gattatttcc ctctttacca gtgtagtagc gaatcaaaag caaaaagagg aatagtatca
    12961 gcagaaaacg cgaaaaatgc tctgatacca tcaaaatcag agctcctgtc agaagacagg
    13021 cttcgataaa taaaaagatt ttaaattttc tcataggttc atcctctccc ttctatttta
    13081 tcacaattca aaaaagtcac ctcagtctga ggatggaaaa aaggcgctgg ttacgccttt
    13141 ttcatctgat cctttgcttc ttttaatttt ccataaagaa gatagtctac tttttgtaga
    13201 tctgctatgg tggcacagtt aagggaacac ataatcaagc gtaggtctgc tttccagcct
    13261 tggacaatgc caatcacttc ttcaactgtg taggtttcaa ccaattccag aacggttcgt
    13321 gacaatccca cagacttagc accaaaaacc aagcacttaa tcatatccag cggattccga
    13381 acccctccac taaccaagag ttcgacctta tctttccatt cttgggcatt gagaagggcc
    13441 tgcatggtag actgacccca ttgattgagg taatcacgct ggccactacg acggttttcg
    13501 atataggcaa agctggtgcc accacgaccc gataggtcca ctgtacgaac accgaattca
    13561 taggctcttt cgattgtctt ggcatccatt ccaaagccca cttccttgag gacaatagga
    13621 acgggaattt gctcgctata atctgctaga tgcgattgcc agcttctaaa cttcctttct
    13681 ccctcgggca tgagtaattc ctgcatgaca ttgacatgca cttgcaatag aacaggattc
    13741 atctcttcta cagtctgaag tcctaactcg acaggcttgt ccaatccaat attggttcca
    13801 aggaggagat tgggatgact agacttgaca gaaaaagaat catccgttgg atttttgagg
    13861 gctgcgctat aagaacccgt tacaaataaa ataccacagg attccgccac ctgagccagc
    13921 ttttgattga tttctcttcc cttattactt ccaccagtca tggcattgat ataaaaagga
    13981 aagtcccact ttcgaccagc aaactctgtc gaaagatcga tttcatccag attgtaaaga
    14041 ggcaaggaag aatgaatcag ctccacctca tcaaagctat tataggaact tttctgctca
    14101 agggcataga ggatatgctc gtccttacga tttgtcgtca tgtcctatcc tttcttgata
    14161 taagagctca atccccagat cggcccaacg attttttaag gttttggttg attgcgcatc
    14221 aaaactcagg gcgatgccac agtcaccacc accagcacca ctactcttgg caacggcctg
    14281 caaatcttga ctggcttctt tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa
    14341 gccttctaaa agcttgctgg ctacttctac ttgctcgata atcttttctg atttcccctg
    14401 ttccaaggct tcctcaaaag aaaccaccgt ttcttttgag gaagttaaaa aattttgatt
    14461 gatattttgc ttgatttgct ggaccatgtg actcgataca gccacttcct tggtccatcc
    14521 cactaagaaa tcacattcta aagttggttt cacttgtgaa attgaaaagc cccaatcacg
    14581 ctccagaact gtcgccaagt tttcttcttc taaccaagca gccaccttct ggcgatcaaa
    14641 tgactggtag agaaccaaat cctctgccac aatacaggca aggtcgccca tggaaccatt
    14701 gtctcctcgc ttgagcaaga cagcgctagt cagcttgaac aagagctcct gatcaacaga
    14761 aacatcatac agagccagta aagccttgac aaccaagaca acgacgctgc cactagaacc
    14821 tagaccaaac tttttccctt ctcgttccat tttgccacag atttctagag aaaaaggtct
    14881 taaattctga ccacgaacag cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa
    14941 gctgtagtca ggattaggcc ttaagtccac tgcgaaatca aacatatctg aatagatacg
    15001 gtagctgtca gaaaaagcaa tctcagccct catatagatg agaatatcct ttatcaaagc
    15061 taactgccct ggctctaaaa tagcatattc acctgcccaa tagagttttc cgcaagtttt
    15121 aacagcaatc atcttgactc aaatcctttg tttttgacac aatcaagcga taacgctgac
    15181 cgaaaatttc tgacaaatac tccaagtctt tctcctgaca gaagacctta acattgggac
    15241 cagcatccat ggtaaagtag caggcctctc ctttctcacg aagctggcga acaaagtcca
    15301 tagcctcata agaggcatcc gtcagataag aaaaggctgg actagcagtc tttgtcgtag
    15361 catgcatagc cagggcattt ttctccgtta attctccaat cttggcaaaa tcattttcct
    15421 tgagataaat cagcatatcc tgatagtcct tctcagactg acgaacccag tcgtcgaaag
    15481 tcgtcgaggt ttccacacaa agtttcatcc cgtcacggct agagattggt tttttcttgt
    15541 cctctagcac caacataatc atagctagtt tcaagtctgt ctctacaggg taaatttctc
    15601 cactatcctt atcccaggct cctagtggtc cataaaaact ccgagaagaa gagcctgagg
    15661 caaatttggc ttcctgtgcc aactgacttc tatccaatcc aagcttgaaa taagcattac
    15721 aagccttgac cagggcggac aaaccactag aacttgagga caggcccgct gccgtaggca
    15781 tattgttttg agtatcgata cggacaaagc cctcaccagc tggacgataa cggtcaataa
    15841 tcttactcat cttggcatgc tcgacctcat tttgtagctg accattgatg taaaattcgt
    15901 cagctgttac attggctggt aaaggcgaca aggtcgtctc tgtatacata ttttccaaag
    15961 ttagagaaat actgctagta gcaggcacca tctctttttc ttttttcttt ccccaatatt
    16021 tgataatagc aatatttgcg taggaacgta ctgttacagg ctctctatcc atgtctgaac
    16081 agctcctttc tcttctaatc tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa
    16141 ggctatgata caacctccta gcccaccacc gctcatcttg gcacccagag caccatggct
    16201 aagagtcgtt tcaaccaaaa agtctgcctc agggctactg actccaattt cttttaaatg
    16261 taaatgcgct tgactgagga tttgtcccag tccttcagca tctttttgtg aaatcgcaac
    16321 ttctgcttgc tgggttaatt ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt
    16381 attttgaacc acttggatgg cttcacgagt atgaccataa acacccgtat cggcaatcac
    16441 caaataggcg gataaatcca tctcaagttc tgtaaatcct acgttcttga taaagcgaat
    16501 aggttggtca ctgagacagg tcttagcatc caaaccacta ggattcatat gagcaatcat
    16561 ctcagctcga ttgaccaaga tttctagtac atcatgaggc agatcagcct gatagtagtc
    16621 aaataccgca cgaatggccg ctatgctgat agccgctgac gaacccatcc cccgtttctc
    16681 agggatagcc gagtcaatct cacaacgaac gcaggtttct gtgatatcca aatactccag
    16741 tgaagcataa accgccatgg acaaggtatc ctcctcataa aggcgccaag gactctctgc
    16801 aggaactacc ttacaggtca cctccacctc caaaagaggc agggaaatgg caggataacc
    16861 gtaaacgacc gcatgttccc ctattaaaat tatcttacta tgtgcctgac cgacaccaac
    16921 tttttttgtc attttttcct tttactagac gaaaaaacgt cttatttttc atacaagtat
    16981 taattctttc ctatctattt tattatattt tcacaaaaaa agcgattgtt tccattcaca
    17041 atcgcttctt tcattattga acccattcgc cattataatt gacaaaatag ccatctacgg
    17101 tcgtattcac tgccaaggca cctgagctat aagcgtagta ccatctgcca ttgacctgga
    17161 accaacctgt cgtcatagaa cgacgaaaga aactccatac cattaagtaa agaggaaagt
    17221 cgtgagggag catgcgccat tgacaacctg ttttagtgac gtacaaagtc tcattaacaa
    17281 gtactcgttt cggtcattta taggtgcggt gtttggagaa atagggttca atcttagccc
    17341 attcttgatc gtttaaatca gtatcatata ctttgcgtgt cataactcta gtttaacatt
    17401 ttttgtgaat acaggttcta aataatcgac cacgaaaatt tcttaagtgg aaaacgcctt
    17461 atgaagtatg ctacgggaaa gttatgcact taatttgaca attcaagat
//
LOCUS       DAVLFB010000034        17504 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLFB010000034 DAVLFB010000000
VERSION     DAVLFB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.034"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            474..2627
                     /locus_tag="VXD58_001052"
     CDS             474..2627
                     /locus_tag="VXD58_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0675767.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEERKTVEDLSLMKGDMTFKQVYYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2640..3989
                     /locus_tag="VXD58_001053"
     CDS             2640..3989
                     /locus_tag="VXD58_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV0675768.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4159..4866
                     /locus_tag="VXD58_001054"
     CDS             4159..4866
                     /locus_tag="VXD58_001054"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV0675769.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4923..8648
                     /locus_tag="VXD58_001055"
     CDS             4923..8648
                     /locus_tag="VXD58_001055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV0675770.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8741..10183
                     /locus_tag="VXD58_001056"
     CDS             8741..10183
                     /locus_tag="VXD58_001056"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV0675771.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10220..11242
                     /locus_tag="VXD58_001057"
     CDS             10220..11242
                     /locus_tag="VXD58_001057"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV0675772.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11239..11784
                     /gene="purN"
                     /locus_tag="VXD58_001058"
     CDS             11239..11784
                     /gene="purN"
                     /locus_tag="VXD58_001058"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV0675773.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11868..12377
                     /locus_tag="VXD58_001059"
     CDS             11868..12377
                     /locus_tag="VXD58_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV0675774.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12402..13949
                     /gene="purH"
                     /locus_tag="VXD58_001060"
     CDS             12402..13949
                     /gene="purH"
                     /locus_tag="VXD58_001060"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV0675775.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14071..15333
                     /gene="purD"
                     /locus_tag="VXD58_001061"
     CDS             14071..15333
                     /gene="purD"
                     /locus_tag="VXD58_001061"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV0675776.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15736..16224
                     /gene="purE"
                     /locus_tag="VXD58_001062"
     CDS             15736..16224
                     /gene="purE"
                     /locus_tag="VXD58_001062"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV0675777.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16211..17284
                     /gene="purK"
                     /locus_tag="VXD58_001063"
     CDS             16211..17284
                     /gene="purK"
                     /locus_tag="VXD58_001063"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV0675778.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            17307..>17504
                     /locus_tag="VXD58_001064"
     CDS             17307..>17504
                     /locus_tag="VXD58_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0675779.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIG"
BASE COUNT         4885 a         3476 c         4277 g         4866 t
ORIGIN      
        1 aaattatgcg agatttaaaa atttaagatt gacagtagtg ttttctgtac catttttatt
       61 taatgctata atgtctatta taaatagttt ttagaaatac taaaattttt ttctttactt
      121 aataatgata tgaaatcatt tcggttttgt taacttgtaa ttcttatatt ttctaggtag
      181 aacgtttgtt ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat
      241 ttgggaagga agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag
      301 tttatactct tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg
      361 actgacttcg tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag
      421 tttcctagtt tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat
      481 ttgggaaacg tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag
      541 ccatggtttt tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta
      601 agacgaccat ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt
      661 ttgagacgcg agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc
      721 cttttgttgc ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgactgggc
      781 aggataagga tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc
      841 cacgtgagcg ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag
      901 actataagcc tcataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga
      961 agcagcgtgg cttgattgcc aatatcgttt tggcaacact cttggtaacc gtgattaaca
     1021 ttgtgggttc ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt
     1081 cgacactagg gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt
     1141 cttacgctca ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt
     1201 tgtcctatat caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca cgcaggacag
     1261 gggagatcgt gtctcgtttt acagatgcta acagtatcat cgatgcgctg gcttcgacca
     1321 tcctttcgat tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt ctattttcac
     1381 aaaataccaa tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct
     1441 ttgcctttat gaagccgttt gaaaagatga atcgggatac catggaagcc aatgcggttc
     1501 tgtcttcttc tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg
     1561 aaagtcagcg ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta
     1621 cctatagtcg agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttgctta
     1681 atgtcggcat tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc
     1741 agttgattac ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca
     1801 atctgcaaac caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc
     1861 tagtagcttc tgagtttgag gagaggaaaa cagttgagga tttgagcttg atgaagggag
     1921 atatgacctt caagcaggtt tattacaagt atggctatgg tcgagacgtc ttgtcggata
     1981 tcaatttaac cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta
     2041 agacgacttt ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc
     2101 tgggtggtgt caatctcaat cagattgata aaaaagccct gcgccagtac atcaactatc
     2161 tgcctcaaca gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca
     2221 aggaggggac gacacaggaa gatatcttac gggcggtcga attggcagag attcgagagg
     2281 atatcgagcg catgccactg aattatcaga cagaattgac ttcggatggg gcagggattt
     2341 caggtggtca acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct
     2401 tgattttgga tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata
     2461 atctcattgc tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc
     2521 ggacagagaa ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg
     2581 atttgcttgc acagggaggc ttttacgccc atttggtcaa tagctagaaa gaggagagga
     2641 tgaaaccaga atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca
     2701 gtagtgtgat tgtacccatg gcccttttgc tcgtgttttt acttggcttt gcaactgttg
     2761 cagagaagga gatgagtttg tccactagag ctactgtcga acccagtcgt atccttgcaa
     2821 atatccagtc aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg
     2881 ttaagaaggg ggatcttttg gttcaatacc aagaaggggc agagggtgtc caagcggagt
     2941 cctatgccag tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa
     3001 agagcctgca agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca
     3061 cctttcgcga ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg
     3121 agaccatcgc gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca
     3181 tcagtcaaac agaggctaaa attcgcgatt accagacagc taagtcagct attgaaacag
     3241 gtacttcctt ggccggtcag aatctagcct actctcttta ccagtcctac aagtctcagg
     3301 gcgaggaaaa tccccaaact aaggttcagg cagttgcaca ggttgaagca cagatttctc
     3361 agttagaatc tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag
     3421 cctatgcgtc agggttaagc agtcaattgg aatcccttaa atcccaacac ttggcaaagg
     3481 ttggtcagga attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg
     3541 tacagggaaa tcttttagac aaggggaaaa ttacggcaag tgaggatggg gtgcttcatc
     3601 ttaatcctga gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt
     3661 atccatcttt ggaaagagaa gggaaagcca aactcacagc ctatctaagt tcaaaagatg
     3721 tagcaagaat caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc
     3781 aactttttct agattctact attacaagta ttgatgcgac agctactaag actgagaaag
     3841 ggaatttctt taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta
     3901 ggtacggggt ggaaggccgt ctacagatga ttacgggcaa gaaaagttat ctacgttatt
     3961 atttggatca atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta
     4021 aactgtgaga aagattcttc ttgcagtttt ttctttacaa tttttgaaaa acatctgcta
     4081 tttattcggt taaattcttg tgttttttgg ttttttgtgg taaaatgtgc tcaagtaata
     4141 cgaaaggcga actttaaaat gtcaaaacaa ttgatctatt cgggaaaagc taaagatatc
     4201 tatacaactg aggatgaaaa tcttattatt tcaacttaca aggaccaggc gactgctttc
     4261 aatggtgtca agaaggagca gattgcaggt aagggagtct tgaataatca gatttcatct
     4321 tttatttttg agaaattaaa tgcggctggt gtggcgactc actttgtgga gaaactttca
     4381 gacacggaac aactcaataa aaaggttaag attattcctt tggaagtcgt gctccgcaac
     4441 tatactgctg gttccttttc aaaacgtttt ggtgtggatg agggaatcgc cttggagact
     4501 ccgattgtcg aattttacta caaaaatgat gatttggatg atccatttat caatgatgag
     4561 catgtaaaat tcctacagat tgcggatgac cagcagattg cctacttgaa ggaagaaacg
     4621 cgtcgtatca atgaactatt gaaagtctgg tttgctgaga ttgggcttaa attgattgac
     4681 tttaagctag agttcggttt tgacaaggat ggcaagatta tcttggcaga cgaattttca
     4741 ccagataact gccgcttgtg ggatgctgat ggcaaccaca tggataagga tgttttccgt
     4801 agaggattgg gagaactaac cgacgtttat gagattgttt gggaaaagtt gcagggttta
     4861 aaataacaac ctcaaggctg tttgggaata ttgcaagagc tgaaataaag gaataagaat
     4921 tgatggataa acgtattttt gttgaaaaaa aggctgattt tcaggtcaag tcagagagtt
     4981 tggttagaga gctccagcac aacttgggac tgtcaagctt gaaaagtatt cgtattgtgc
     5041 aagtatatga tgtatttgac ttggttgagg acttgtttgc acctgcagag aagcacattt
     5101 tctctgagca ggtaaccgac catgttttag atgaagtatc tgtgcaggcg gatcttgcta
     5161 actatgcttt ctttgccatt gaaagtctac cagggcagtt tgaccagcgt gcagcttcgt
     5221 cacaggaagc cttgcttttg ttgggaagtt cgagtgacgt gacagtcaac acagcccaac
     5281 tttacttggt gaataaagat attgatgcga ctgagttgga agctgtcaaa aactacctgc
     5341 tcaatccagt tgattctcgt ttcaaggata tcacgatagg gattgccaag caggagtttt
     5401 cagagtcaga caagaccatt ccaaaattgc ctttctttga aagctataca gcagaagact
     5461 ttgctcgcta caaggccgaa caagggatgg ccatggaagt ggatgatttg ctctttatcc
     5521 aagactactt taagtcaatc gggcgcgtgc caactgagac tgaactcaag gttttggaca
     5581 cttactggtc tgaccactgc cgtcatacga cttttgagac agagttgaaa cacatcgact
     5641 tttcagcttc taaatttcaa aagcaattgc agtcaaccta tgacaagtat attgccatgc
     5701 gcgaggaatt aggtcggtct gaaaaaccac aaaccttgat ggatatggcg actattttcg
     5761 gtcgttatga gcgtgctaat ggacgattgg atgatatgga agtctctgac gaaatcaatg
     5821 cctgctcagt tgaaattgaa gtggacgttg atggtgtcaa ggaaccttgg ctcctcatgt
     5881 ttaaaaacga aactcacaac catccaacag aaattgagcc atttggtgga gcggctacct
     5941 gtatcggtgg agctattcgt gatccgttgt caggccgttc ctatgtttac caagccatgc
     6001 gtatttcagg tgctggtgat attacagcac cgatttcgga aactcgtgct gggaaattgc
     6061 cacaacaagt catttctaaa acagcaactc atggttattc ttcatatggt aaccagattg
     6121 ggcttgccac aacctacgtt cgtgaatact tccacccagg ctttgtagct aaacgtatgg
     6181 aacttggtgc cgttgttggt gcggctccca agggcaatgt tgtccgtgaa aaacctgaag
     6241 caggtgatgt gatcatcctt ctcggaggta aaacaggtcg tgatggtgtc ggtggtgcga
     6301 cgggctcttc taaggttcaa acagttgagt ctgtagagac tgctggtgct gaggttcaaa
     6361 aaggaaatgc catcgaagaa cgcaagattc agcgcctctt ccgtaatggc aatgtcactc
     6421 gtctgatcaa gaagtccaat gactttgggg caggcggtgt ctgtgtggct atcggtgaat
     6481 tggcagacgg tcttgaaatc gacctcaaca aggtgcctct taaataccag ggcttgaatg
     6541 gtacagaaat tgccatctct gaatcacaag aacggatggc ggtcgtggtt cgtcctgaag
     6601 atgtggatac cttcgttgcc gaatgtaaca aagaaaatat tgatgctgtt gtggtggcga
     6661 cagtaactga aaaaccaaat cttgtcatgc actggaatgg tgagacaatc gttgacttgg
     6721 agcgtcgttt ccttgacatc aatggtgtgc gcgtggttgt cgatgccaaa gttgtggaca
     6781 aggatgtcaa actcccagaa gagcgtcaaa catctgctga aacactggaa tcagataccc
     6841 ttacggttct atctgacctc aaccatgcaa gtcaaaaagg attacagact atctttgact
     6901 gctctgttgg acgctcaacg gttaatcacc cacttggtgg tcgttaccaa ctcacaccaa
     6961 ctgaggcatc tgtgcagaaa ttgccagttc aacacggtgt gactcatact gcgtcggtca
     7021 ttgctcaagg tttcaaccca tatgtagctg aatggtctcc ataccacggt gctgcttatg
     7081 cggttatcga agcaactgct cgtttggtgg ctgctggtgc caactggtcc aaggctcgtt
     7141 tctcttacca agaatatttc gagcgtatgg acaagcaagc agagcgtttt ggtcagccag
     7201 tagccgctct tctaggctct attgaagctc agattcaact tggcttgcca tctatcggtg
     7261 gtaaggactc catgtctggt acctttgaag aattgaccgt tccgccaacc ttggttgcct
     7321 ttggggtgac gacggcagat agccgtaagg tgctctctcc agaatttaaa gctgttgggg
     7381 aaaatatcta ctacatccca ggtcaagccc tctctgcaga gattgatttt gacttgatta
     7441 agaaaaattt tgctcagttt gaagcaagcc aagctgacca taaagtgaca tctgcatcag
     7501 ctgtcaaata cggtggtgta gttgaaagtt tggctcttgc tacctttgga aactatattg
     7561 gtgcagaggt gaccttgcct gaacttaaaa cagctttgac agctcaatta ggcggctttg
     7621 tcttcacatc tcctgaagaa attgctggag tagagaagat tggacaaacg aaagcagact
     7681 ttacactgac tgtcaacggt gtgaagctag atggacacaa gcttgacagt gcatttcaag
     7741 ggacattgga agaagtttac ccaacagaat ttacccaagc gaaagaacta gaagaagtac
     7801 cagctgtggc atcagatgtt gtgattaaag ccaaagaaaa ggttgaaaaa cctgtggttt
     7861 acatcccagt ctttccagga accaactcag aatatgattc agctaaggcc ttcgaaaaag
     7921 aaggtgcaga ggtcaatttg gtgccattcg tgaccttgaa tgaagaagct attgtcaagt
     7981 cagttgaaac tatggttgac aatatcgaca agactaatat tctcttcttt gctggtggat
     8041 tctcggctgc ggatgaacca gatggttcag ctaagtttat cgtcaatatc ctgcttaatg
     8101 aaaaagtgcg tgtggctatt gatagcttta tcgcccgtgg tggtttgatt atcggtattt
     8161 gtaatggatt ccaagcctta gtcaaatcgg gtctcctacc ctacggaaac tttgaagatg
     8221 ctaacaatac tagcccaacc ctcttctaca atgatgccaa ccaacacgtg gccaagatgg
     8281 tggaaactcg cattgccaat accaactcac catggttggc tggtgtgcaa gtgggcgata
     8341 tccacgctat tcctgtttcg cacggtgaag ggaagtttgt cgtgacggct gaggaatttg
     8401 cagagctccg tgacaatgga caaattttca gccaatacgt tgactttaac ggtaaaccaa
     8461 gtatggattc taagtacaat ccgaatggtt ctgtccatgc catcgaagga attaccagca
     8521 agaatggtca aatcatcggt aagatgggcc actcagaacg ttatgaggat ggtcttttcc
     8581 aaaatatccc aggcaataaa gaccaacacc tgttcgcatc agcggttaaa catttcactg
     8641 gaaaataaga cttacagatt ttctaataga tagtatcagt aatgtaaaag tcatgtaaat
     8701 ctagctcttg atgattacaa atgaaaatta ggtataaaaa atgacatacg aagtaaaatc
     8761 tcttaatgaa gaatgtggtg ttttcggtat ttggggacat ccagatgctg ctaagttgac
     8821 ctattttgga ctccacagtc ttcaacaccg tggtcaggag ggggcaggaa tcctctccaa
     8881 tgatcaagga caactgaagc gccatcgtga catggggctt ttatcagaag ttttcagaaa
     8941 tccagccaat ttggataaat tgacaggagc tggtgcgatt gggcatgtgc gttatgcgac
     9001 tgctggcgaa gcttctgttg ataacatcca gcccttcctc ttccgttttc acgatatgca
     9061 gtttggcttg gctcataatg gaaatctgac caatgcagcc tctctcaaga aagaactgga
     9121 acaaagagga gcaattttca gcgcgacttc ggactctgaa atcttggctc acctcattcg
     9181 tcgcagtcat aatcctagcc tgatgggcaa aatcaaggaa gcgctcagcc ttgtcaaagg
     9241 tggttttgcc tatatcttgc tgtttgagga caagttgatt gcggctcttg accctaatgg
     9301 ctttcgtccg ctttctatcg ggaaaatggc caacggagcg gtggttgttt cctctgaaac
     9361 ctgtgctttt gaggtcattg gtgccgagtg gattcgtgat ttgaagccag gtgagattgt
     9421 gatcattgat gacgagggca ttcagtatga cagctataca gatgatactc agttggcgat
     9481 ttgttctatg gagtatatct actttgctcg ccctgattct aatatccacg gtgtcaatgt
     9541 ccatacggca cgtaagagaa tgggagcgca attggcgcga gaatttaagc atgaggcaga
     9601 tattgtagtt ggtgtgccca attcttccct aagcgcggct atgggatttg cggaagaatc
     9661 aggcttacca aatgaaatgg gtctgatcaa aaaccaatac acccagcgaa cttttatcca
     9721 accgactcaa gaattgcggg agcaaggagt gcggatgaaa ctgtctgctg tttcgggtgt
     9781 tgtcaaaggc aaacgtgtgg tcatggtgga tgattccatt gtacgtggaa caacctctcg
     9841 tcgtatcgtt cagctcttga aagaagcggg tgcgactgag gttcacgttg ccattggaag
     9901 tcctgcacta gcgtatccat gtttctacgg gattgatatc cagacccgtc aggagctgat
     9961 tgcagccaat catacggtcg aagaaactcg ccaaatcatt ggtgcggaca gtctgactta
    10021 tctttcaatt gatggcttga ttgagtcgat tggtatcgaa acagatgcgc cgaacggtgg
    10081 tctctgtgtc gcttactttg acggtgacta cccaacgcct ctctatgact acgaagaaga
    10141 ctatcgtaga agtttggaag aaaagaccag tttttacaag taggcgacag attctccatt
    10201 aaagaaaagg aaaaaacaaa tgacaaataa aaatgcatat gcccagtcgg gtgtggatgt
    10261 tgaagcgggt tatgaagttg ttgaacggat taaaaagcac gtggcccgta cggagcgtgc
    10321 aggtgtcatg ggagctcttg gtggctttgg tggtatgttt gacctttcca agactggggt
    10381 taaagaaccc gtcttgattt cagggactga cggtgtcgga accaagctca tgttggctat
    10441 caagtacgac aagcacgata ccatcgggca ggactgtgtg gccatgtgtg tcaacgacat
    10501 cattgctgca ggtgcggaac ccctctattt tctcgactac gtagcgacag ggaagaatga
    10561 accagctaag ctagaacaag tggttgctgg tgtggcagaa ggttgtgtgc aggctggtgc
    10621 tgccctcatc ggtggggaaa cggctgaaat gccgggcatg tacggcgaag acgactatga
    10681 cttggctggt tttgcggtcg gtgtggctga aaaatctcaa atcattgacg gttcaaaggt
    10741 ggtagaggga gatgttcttc tcggacttgc ttcaagtggg attcactcaa atggttactc
    10801 tttggttcgt cgtgtctttg cggattacac aggtgaggaa gtcctaccag aattggaagg
    10861 caagaaactt aaggaagttc tacttgagcc gactcgtatc tatgtcaagg ctgtcttgcc
    10921 gctcatcaaa gaagagttgg tcaacggcat tgcccacatc acaggtggtg gctttatcga
    10981 aaatgtccct cgtatgtttg cagatgacct agctgctgaa attgatgaaa gcaaagttcc
    11041 agtgcttcca attttcaaag cccttgaaaa atatggtcag attaaacacg aagaaatgtt
    11101 tgaaatcttc aatatgggtg tgggacttat gttggcggtc agccctgaaa atgtagagcg
    11161 tgtaaaagaa ttgttggatg aagcagtcta tgaaattggt cgcatcgtca agaaagaaaa
    11221 cgaaagtgtc attatcaaat gaaaaaaata gcggtttttg cctctggtaa tggctcaaat
    11281 tttcaggtga ttgccgaaga atttccagtg gagtttgtct tttcagacca tcgtgatgcc
    11341 tatgtgcttg agcgtgcaaa gcagctcggc gttctgtcct atgcttttga actcaaggag
    11401 tttgagagca agacagacta cgaagcagcc cttgtcgaac tcttggaaga acaccagatt
    11461 gacttggttt gcctagcagg ctacatgaaa atcgttggac caaccttatt gtcggcttat
    11521 gaaggtcgga ttgtcaacat tcatccagcc tacttgccag aatttccagg agctcatggg
    11581 attgaggatg cttggaatgc tggcgtgggt cagtctggtg tgaccattca ctgggtggat
    11641 tcgggtgtgg atacaggcca ggtcatcaaa caggttcgtg tgccacgact agctgatgat
    11701 accattgaca gatttgaagc tcgcatccat gaagcagagt acaggttgta tccggaagta
    11761 gtgaaggctc tatttacaga ttgacttttt gatgattcat atgatatctt tgattttaaa
    11821 ttggagtcag tgtttgttga agacggcttc aaacggaggt atttgtaatg ttagaatcta
    11881 aaaaaacaac tcgatatgta ttttatgtct atctgatgtt attaacttgg ggaatcttat
    11941 ttaagtttga aacaaatcct gaatttatag catttttctt agctccaagg tatatcaatt
    12001 ggattccatt ttcagaacca ctaatagtcg atggaaaaat tgtttttgct gaaatgttat
    12061 ttaatctgat ttcctttatt ccattaggtg tttgtttccc tttgataaaa actaatttat
    12121 ctagtttaag aatagtcggg acaggtttct tgattagttt attgtttgag tgcttacagt
    12181 atattttagc aataggtata acagatataa cggatttgac tttaaatacg ctaggtgtct
    12241 gtgtaggctt actgatttat caaattttta taagagtgtt caaatcacag actagaaaat
    12301 ggatcaatat cttaggtatg cttagccttg gttttgctta tcttgtttta ctgttactgc
    12361 atttaattgg tgtttaacta atgattaaaa aggagaatat aatgactaaa cgcgtcttaa
    12421 tcagcgtctc agacaaagcg ggcattgttg aatttgccca agaactcaaa aaacttggtt
    12481 gggagattat ctcaacaggt ggaactaagg ttgcccttga taatgctggg gtggatacca
    12541 ttgctatcga tgatgtgact ggtttcccag aaatgatgga cggtcgtgtg aagaccctcc
    12601 acccaaatat ccacggaggg cttctcgctc gtcgtgactt ggatagccac ttggaagcgg
    12661 ctaaggacaa caagattgag ctcattgacc ttgtggtggt caacctttac ccatttaagg
    12721 aaactatcct taaaccagat gtgacttatg ctgatgcagt tgaaaatatc gatattggtg
    12781 ggccgtctat gcttcgttca gcagcgaaaa atcatgccag tgttacagtt gtggtagatc
    12841 ctgctgacta cgctgtggtt ttggacgaat tggcagcaaa cggcgaaacc tcttatgaaa
    12901 ctcgccaacg tttagcagcc aaagtatttc gtcacacagc ggcttatgac gccttgattg
    12961 cagaatactt cacagctcaa gtgggtgaaa gcaagcctga aaaactcact ttgacttatg
    13021 acctcaagca accaatgcgt tacggtgaga atcctcaaca agacgcggac ttttaccaga
    13081 aagctttgcc tacagattac tctattgctt cagccaaaca gctcaacggg aaagaattgt
    13141 catttaataa tatccgtgat gcagatgctg ctatccgtat catccgtgac ttcaaagata
    13201 gtccaaccgt tgtggctctc aaacacatga atccatgtgg aattggtcaa gctgatgaca
    13261 tcgagactgc ttgggactac gcttatgagt ctgacccagt atctatcttt ggtgggattg
    13321 tcgtcctcaa ccgtgaggtg gatgctgcga cagctgagaa gatgcacggc gttttcctcg
    13381 aaatcatcat tgcaccaagc tatacggatg aagcgctagc tattttgatc aataaaaaga
    13441 aaaacttgcg tatccttgcc ttgccattta atgctcaaga ggctagcgaa gtggaagcag
    13501 aatacacagg tgtagtcggt ggacttctcg tgcaaaatca agacgtggtc aaggaaagcc
    13561 cagctgactg gcaagtggtg accaaacgtc agccaactga gacagaagcg actgctcttg
    13621 agttcgcttg gaaggctatc aagtacgtca aatcaaatgg tattatcgtg accaacgacc
    13681 acatgacact tggtgttggt ccaggtcaaa ccaaccgtgt ggcttctgtt cgccttgcca
    13741 ttgaccaagc caaagatcgt ctgaacgggg cggtccttgc ttcagatgcc ttcttcccat
    13801 ttgcggataa cgtggaagaa atcgccaaag caggaattaa ggccatcatc cagcccggtg
    13861 gctctgtccg tgaccaagaa ttcatcgaag cagcggataa atacggcttg actatggtct
    13921 ttacaggtgt gagacatttt agacattaag aagataaaag ggaagaaaac agtttctttc
    13981 cttttttggc ttaaaatgct aactgaaaca agattaaaac gaactttttt gatataatgt
    14041 tggtaaataa ttcgcaaaag aggttgagga atgaaactgc ttgttgtcgg ttctggtggt
    14101 cgtgagcatg cgattgctaa aaagttactt gaatcaaaag acgtggaaaa agtctttgta
    14161 gctcctggga atgacggtat gactctggat ggtctggaat tggtaaatat atctatttcc
    14221 gaacattata aattgattga cttcgcaaag gccaacgata ttgcttggac ctttatcggt
    14281 ccagatgacg cccttgcggc tggtatcgtg gatgatttcc atgcagctgg tctcaaagcc
    14341 tttggtccga caagattggc agcggagctg gagtggtcca aggatttcgc caaggaaatc
    14401 atggtcaaat atggtgtttc gacagcagcc tatggcacat tttcagattt cgaggaagca
    14461 aaggcctata tcgaaaagca tggtgcgcct atcgtagtca aggcggatgg cttggcgctt
    14521 gggaagggtg tcgtcgttgc tgagacggtt gagcaagcgg tcgaagccgc tcatgagatg
    14581 cttttggaca ataaatttgg tgactcaggt gcgcgcgtgg ttattgagga attccttgaa
    14641 ggagaggaat tttcactctt tgcctttgtc aatggtgata agttctacat catgccaacg
    14701 gctcaggacc acaaacgtgc ctacgatggc gacaaagggc ctaacacggg tggtatgggt
    14761 gcctatgcgc cagttccaca cttaccacag agtgtagttg atacagcggt tgacaccatt
    14821 gtcaagccag ttctagaagg gatgattaaa gaaggtcgcc cttatctggg agttctttac
    14881 gcagggctta tcctgacagc tgatggaccg aaagtcattg agttcaacgc tcggttcgga
    14941 gatccagaaa ctcagattat cttgcctcgc ttgacctctg actttgctca aaatatcaca
    15001 gatatcctgg atagcaagga gccaaatatc atgtggacgg acaagggtgt gactctgggt
    15061 gtggttgtcg catccaaggg ctacccgcta gactatgaaa ggggcgttga gttgccagcc
    15121 aagacagaag gcgatgtcat cacctactat gcaggggcta agtttgcgga aaatagcaga
    15181 gcactgctct caaacggcgg acgagtttat atgctcgtta ccacagcaga taccgtcaaa
    15241 gaagcccaag ccagcatcta ccaagaacta tcccaacaaa aaatagaagg actcttctac
    15301 cgaacagata tcggaagcaa ggcaattaag taaagatata agaataacgc gccgtagtcg
    15361 ccaaacacga taatggtcgt cgtggtgaaa agaccagaac agtgaatgtt ctggtcaggg
    15421 ggaaacttgg agaccgtagg ctcaaagttt aggaatgaaa ccgaaggttt gcttccgcct
    15481 ccatcaccta agaccattat caaaaagaaa aataaaaatt cacaaaatac gttaatgatc
    15541 gtatggtttg cgagcgttag cgagctaata tagaacaatc accgccgttg tgaaagaacg
    15601 attggatgat aatccaatcg ttcagggaaa ttggaagacc ttgggtttcc aatttaggca
    15661 tgagacacct ttggtggctg ctgccgtccc tcacaagcta aggtgattgt tgaaaaagag
    15721 gaaaaaggag aagaaatgaa accagtaatt tccatcatca tgggctcaaa atccgactgg
    15781 gcaaccatgc aaaaaacagc agaagtccta gaccgcttcg gtgtagccta cgaaaagaaa
    15841 gttgtttccg cacaccgtac accagacctc atgttcaaac atgcagaaga agcccgtagt
    15901 cgtggcatca agatcatcat cgcaggtgct ggtggcgcag cgcatttgcc aggcatggta
    15961 gctgccaaaa caacccttcc agtcattggt gtgccagtca agtctcgtgc tcttagtgga
    16021 gtggattcac tctattctat cgttcagatg ccgggtgggg tgcctgttgc gaccatggct
    16081 atcggtgaag ctggagcgac taacgcagct ctctttgccc tccgtctcct ctctgtagaa
    16141 gataagtcca ttgcggatgc acttgccaac tttgctgaag aacaaggaaa aatcgcagag
    16201 gagtcgtcaa atgagctcat ctaaaacaat cggaattatc ggtggcggtc aactgggtca
    16261 gatgatggcc atttctgcta tctacatggg ccacaaggtt atcgcgctgg atcctgcggc
    16321 ggattgcccg gcctctcgcg tggcggaaat cattgtggca ccttataacg atgtggacgc
    16381 cctccgtcag ttggcagacc gttgcgatgt cctcacttat gagtttgaaa atgtcgacgc
    16441 tgacggtttg gatgccgtta tcaaggatgg acaactccct caaggaacag atctgctccg
    16501 catttcgcaa aatcgtattt ttgaaaagga ctttttgtca aacaaggctc aagtcactgt
    16561 ggcaccctac aaggtcgtga cttctagcct agacttggca gatatcgact tgtcgaaaaa
    16621 ctatgtcctc aagactgcga ctggtggcta cgatggtcat ggacaaaagg ttattcgttc
    16681 agaagcagac ttggaagcag cctatgcgct agcagactca gcagactgcg tcttggaaga
    16741 atttgtcaac tttgaccttg agatttctgt catcgtgtca ggaaatggca aggaggtgac
    16801 gtttttccca gttcaggaaa atatccaccg caacaatatc ctgtctaaga ccatcgtacc
    16861 agcccgcatt tctgaaagtc tagtagacaa ggctaaagct atggcagtgc gaatcgcaga
    16921 acaactcaac ttgtctggaa ctctctgtgt ggaaatgttt gcgactgatg atgacgttat
    16981 tgtcaatgaa atcgccccac gaccacataa ctctgggcac tattctattg aagcctgtga
    17041 tttctctcag tttgacaccc atattctcgg tgttctcgga gcaccattac cagtcatcaa
    17101 actccatgcg cctgccgtca tgcttaacgt tctcggccag catgttgagg ccgctgaaaa
    17161 atatgtcaca gaaaattcaa gcgcccacct ccacatgtat ggtaaaatag aagcgaagca
    17221 caaccgcaag atgggacatg tgactttgat tagtaatgaa ccagatagtg tgggagagtt
    17281 ttaggggaga ggggtctaaa gaaaaaatgt ttgaaaaaat taaaggaatt aatataaaat
    17341 caggtatttt tgaagatgaa acaaaattag aactctttga aggaaatttt gagggaacaa
    17401 atccagtaca aaacgatagg gcaagtttat tgtttggaag gaatggttca gggaaaagta
    17461 ccattgctcg aggaataaat caattaaaaa atggtgagat tgga
//
LOCUS       DAVLFB010000035        17184 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLFB010000035 DAVLFB010000000
VERSION     DAVLFB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.035"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(23..1510)
                     /gene="tacF"
                     /locus_tag="VXD58_001065"
     CDS             complement(23..1510)
                     /gene="tacF"
                     /locus_tag="VXD58_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV0675780.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            1846..2553
                     /locus_tag="VXD58_001066"
     CDS             1846..2553
                     /locus_tag="VXD58_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV0675781.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            2558..3580
                     /locus_tag="VXD58_001067"
     CDS             2558..3580
                     /locus_tag="VXD58_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV0675782.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            3597..4466
                     /gene="licA"
                     /locus_tag="VXD58_001068"
     CDS             3597..4466
                     /gene="licA"
                     /locus_tag="VXD58_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV0675783.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            4456..5334
                     /locus_tag="VXD58_001069"
     CDS             4456..5334
                     /locus_tag="VXD58_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV0675784.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            5346..6035
                     /locus_tag="VXD58_001070"
     CDS             5346..6035
                     /locus_tag="VXD58_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV0675785.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            6131..6979
                     /gene="dprA"
                     /locus_tag="VXD58_001071"
     CDS             6131..6979
                     /gene="dprA"
                     /locus_tag="VXD58_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV0675786.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            7868..8911
                     /locus_tag="VXD58_001072"
     CDS             7868..8911
                     /locus_tag="VXD58_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV0675787.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            9078..11165
                     /gene="topA"
                     /locus_tag="VXD58_001073"
     CDS             9078..11165
                     /gene="topA"
                     /locus_tag="VXD58_001073"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV0675788.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            11271..11630
                     /locus_tag="VXD58_001074"
     CDS             11271..11630
                     /locus_tag="VXD58_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV0675789.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            11723..12355
                     /locus_tag="VXD58_001075"
     CDS             11723..12355
                     /locus_tag="VXD58_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV0675790.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            12399..13055
                     /locus_tag="VXD58_001076"
     CDS             12399..13055
                     /locus_tag="VXD58_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV0675791.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            13074..13706
                     /locus_tag="VXD58_001077"
     CDS             13074..13706
                     /locus_tag="VXD58_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV0675792.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            13734..14168
                     /locus_tag="VXD58_001078"
     CDS             13734..14168
                     /locus_tag="VXD58_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV0675793.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            14180..15217
                     /gene="leuB"
                     /locus_tag="VXD58_001079"
     CDS             14180..15217
                     /gene="leuB"
                     /locus_tag="VXD58_001079"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV0675794.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            15214..15483
                     /locus_tag="VXD58_001080"
     CDS             15214..15483
                     /locus_tag="VXD58_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV0675795.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            15487..15846
                     /gene="leuD"
                     /locus_tag="VXD58_001081"
     CDS             15487..15846
                     /gene="leuD"
                     /locus_tag="VXD58_001081"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV0675796.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            16008..16631
                     /locus_tag="VXD58_001082"
                     /pseudo
     CDS             16008..16631
                     /locus_tag="VXD58_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            16797..>17184
                     /locus_tag="VXD58_001083"
     CDS             16797..>17184
                     /locus_tag="VXD58_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675797.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEII"
BASE COUNT         5432 a         2922 c         3681 g         5149 t
ORIGIN      
        1 ttagttgttt cattttaaac tcctatgatt ttttaaattt attttttaag gctagaacat
       61 ggtttaaaaa ttcatagaaa atgctatctt ttgtgaagac aagtagacta atataagaga
      121 tagctgatag caagataatc aaaccagtat taatcaaaaa tggcaaatta atgaccatat
      181 ccacaggata cacgaaatta atcaggaaat aaattgctac aaaggaaagt gaaaagagag
      241 agtatcgaac agtatagcta aagatatgtc ccaagtggat gagttgtttt ctatggatga
      301 aaatgatata gaaaacaagt agagaagcct ctgataacat agttgtcagt aagtagtatt
      361 cgggagccac gatacggttg aaaaagagga gactattcaa gcccaaattg agtagcccag
      421 caaagactgt ataaactgtg atacgttttt catagccatt ggtaaagaga atttgggaac
      481 caagaatggt atctaaggcc aggataatcg tacgaaaagc gaagagagag gtcaagatgc
      541 cgcctccgat atatttttca ctaccgtaaa gtaggatggc atttggtcct aaaaccatga
      601 gtccaaaact cagtggaatg ataaagaagt taaagattcg actacctcta ttaaccagag
      661 aaacataggc ttctttgtct cctttcccca gatagtaact gagacgaggc acactcactc
      721 caattgcacc tgttacaacc ccagctataa cggtcacaat tcgctgagct atggtatagt
      781 aactaacgtt gacatcaatc cctgttttaa cgaggaagag gcgatctaaa aaagtgaaga
      841 gcatattggc attggcaaag actaacatgg ctgtcagagg gagaaagagt ggtttaaaat
      901 cacttaggtg aatcttaaca agtttgatgt ctcttttaat ccaaaaataa ctaatcaggt
      961 agttaatcag cgtcgataaa ctcatcacaa gtgtatagac aacaatatcg tgttcatttt
     1021 taacaaataa gaaaatagag accagcatca ggatacggat gaaggcagtt ttgtaaaaga
     1081 gaaaactgta attttccaga gcttcattga cccattcgat tgaaaaaatc tgggcaatga
     1141 gttgaatccc cataacaagg tagacctttt tgatgattgg attatcagta aagaagagag
     1201 gataggctag gatatagaca gcagtggtca aaatcgtaca agcgatgcac aaataaaaaa
     1261 gactagaaaa ggttctgtta agatcttttt tgttatcctt gacattactg atagccctta
     1321 aaccgtagtt atagacacca taagttgcaa agggcaagaa aaatgacaaa atagtgtcga
     1381 ctgagttgaa gtaaccatag tcagttcggt ccaagacacg cgcgacatag gttccagtta
     1441 ggatgggaaa aataatattc aagacacgaa ttcccatgta agatagagca tttaatttta
     1501 tacttttcat tcaatttacc tcgtttttca ttatatcata aagttagcta ataagaaatg
     1561 aagggcagta agccaagcaa tcactttgaa gtttcaaatc ttaaatttta agttttcttt
     1621 aaggaaagta tattattctg aaggactcta aaatttcgca gtcatttatt agtaattgct
     1681 acagaattcc tagtcattac tagaaatgga ctagtttctt tgaataatag aactgcataa
     1741 ttctcctatt ctagaagggg aggaccagta tttcttttat gataggacta gattgtggta
     1801 taatagagag aataagtttt tttagtaaga caaaggagaa aatagatgat ttatgcagga
     1861 attcttgccg gtggaactgg cacacgcatg gggatcagta acttgccaaa acaattttta
     1921 gagctaggtg atcgacctat tttgattcat acaattgaaa aatttgtctt ggagccaagt
     1981 attgaaaaaa ttgtagttgg tgttcatgga gactgggttt ctcatgcaga agatcttgta
     2041 gataaatatc ttcctcttta taaggaacgt atcatcatta caaagggtgg tgctgaccgc
     2101 aatacaagta ttaagaaaat cattgaagcc attgatgctt atcgtccgct tactccagag
     2161 gatatcgttg ttacccacga ttctgttcgt ccatttatta cacttcgcat gattcaggac
     2221 aatatccaac ttgcccaaaa tcatgacgca gtggacacag tggtagaagc agttgatact
     2281 atcgttgaaa gtaccaatgg tcaatttatt acagatattc caaatcgtgc tcacctttat
     2341 caaggacaaa cacctcaaac attccgttgc aaggacttca tggaccttta tggatctctt
     2401 tctgatgaag agaaggaaat cttgacagat gcatgtaaaa tctttgtgat caaaggaaaa
     2461 gatgtggcct tggccaaagg tgaatactca aatctgaaga ttacaaccgt aacagatttg
     2521 aagattgcaa aaagtatgat tgagaaagac tagtaaaatg attaatcaaa tttatcaact
     2581 aactaagcct aagtttatca atgtcaaata tcaggaagag gctattgacc aagagaatca
     2641 tatccttatc cgtcccaact acatggctgt ctgtcatgcg gatcagcgtt actatcaggg
     2701 aaaacgtgat cccaagattt tgaataaaaa gcttccaatg gcaatgattc acgagtcatg
     2761 tggaaccgtc atttctgacc cgaccggaac ctacgaggtt ggtcaaaaag ttgtcatgat
     2821 tcccaatcag tctcctatgc agagtgatga agaattctat gaaaactaca tgacagggac
     2881 ccatttcttg tctagtggat ttgatggctt tatgagagag tttgtttctc tccctaaaga
     2941 tcgtgtggtg gcttatgatg ctattgaaga tacggttgca gccattacag agtttgtcag
     3001 tgtgggcatg cacgctatga atcgtctatt gactcttgct catagcaagc gggagcggat
     3061 cgccgttatt ggagatggaa gtttagcttt tgtggttgcc aatattatca actatacttt
     3121 gccagaatca gagattgtgg ttattggtcg tcattgggaa aagttggaac tcttctcatt
     3181 tgccaaagaa tgctatatta cggataatat tcctgaagag ttggcctttg accatgcttt
     3241 tgaatgttgt ggtggtgatg gtactggacc agctattaat gacttgattc gctacattcg
     3301 tcctcaggga acgattctca tgatgggagt tagcgaatat aaagtcaatc tcaatactcg
     3361 cgatgcctta gaaaagggct tgattttggt tgggtcatct cgttctggtc gcattgattt
     3421 taaaaatgct atccaaatga tggaagtcaa gaaatttgcc aatcgtctta aaaatatcct
     3481 ttatctagaa gaacctgtaa gagaaattaa agatattcat cgtgtctttg caaccgattt
     3541 aaacacagcc tttaaaacag tgtttaagtg ggaagtataa gtactggagg ttaattgtgg
     3601 agaaaatcat taaagaaaaa atttcttcct tacttagtca agaagaggaa gtcctcagtg
     3661 ttgaacaact gggtggaatg accaatcaaa actatttggc caaaacaaca aataagcaat
     3721 acattgttaa attctttggt aaagggacag aaaagcttat caatcgacaa gatgaaaagt
     3781 acaatcttga actactaaag gatttaggct tagatgtaaa aaattatctt tttgatattg
     3841 aagctggtat caaagtaaat gagtatatcg aatctgcgat tacgcttgat tcaacgtcaa
     3901 tcaagaccaa gttcgacaaa attgctccaa tattacaaac tattcatacg tctgctaagg
     3961 aattaagagg agaatttgct ccttttgaag aaatcaaaaa atacgaatcc ttgattgaag
     4021 aacaaattcc ttatgccaac tatgaatctg ttagaaatgc agtcttctcc ttagagaaaa
     4081 gactggctga cttaggtgtt gacagaaaat cttgtcatat cgatttggtg cctgaaaact
     4141 ttatcgaatc acctcaagga cgactttatt tgattgactg ggaatattca tcaatgaatg
     4201 atccaatgtg ggatttggct gccctctttt tagagtctga attcacttcc caagaggaag
     4261 aaactttctt atctcactat gagagtgacc aaacaccggt ttctcatgaa aagattgcta
     4321 tttataaaat tttacaagat actatttgga gtctatggac tgtctataag gaagagcaag
     4381 gtgaagattt tggtgactat ggtgtgaatc gttaccaaag agctgttaaa ggtttggctt
     4441 cttatggagg ttcagatgaa aagtaaaaac ggagttcctt ttggccttct ctcaggtatt
     4501 ttctggggct tgggtctaac ggttagtgct tatatctttt cgatttttac agatttgtca
     4561 ccctttgtgg tggctgcagc tcatgatttt ttgagcatct ttatcttact agcttttctc
     4621 ttggtaaaag aagggaaagt tcgcctctca attttcttaa atattcgcaa tgtcagtgtt
     4681 atcatcggag ccttgctagc aggccctatc ggtatgcagg ccaatcttta tgcagttaag
     4741 tatatcggaa gttctttagc ttcatctgta tcggctattt accctgcgat ttcagttcta
     4801 ttggctttct tctttttgaa gcacaagatt tcgaaaaata ctgtatttgg gattgtcttg
     4861 attattggag ggattattgc tcagacctat aaggttgaac aggttaattc tttctacatt
     4921 gggattcttt gtgctttggt ttgtgctatt gcatggggaa gtgagagtgt tcttagctct
     4981 tttgccatgg aaagtgaatt gagtgaaatc gaagccctct taatccgtca agtaacttcg
     5041 ttcttgtcct atcttgtgat tgtgctcttc tctcatcagt catttactgc agtagccaat
     5101 ggacaattgc taggtctcat gattgttttt gcagcctttg atatgatttc ctacttggct
     5161 tattatatcg ctatcaatcg cttgcaacca gccaaggcta caggcttgaa cgtgagctat
     5221 gtagtatgga cggtcttgtt tgcagttgtt ttcttgggtg caccgctaga tatgctgacc
     5281 attatgacgt cacttgtcgt cattgctgga gtttatatta ttattaaaga ataaaggaga
     5341 ttcgtgtgaa agccattatc ttagcagcgg gattgggaac tcgcttgcgt cctatgactg
     5401 aaaatacccc taaagccttg gttcaggtta atcaaaaacc tttgattgag taccaaattg
     5461 agtttctcaa agaaaaagga atcaatgaca tcatcatcat tgttggttat cttaaagaac
     5521 aattcgatta cttgaaagag aaatacggtg ttcgtctcgt tttcaatgat aaatacgctg
     5581 actacaataa cttttactct ctctatcttg taaaagaaga attggccaac agctatgtta
     5641 ttgatgctga caattatctc tttaaaaata tgttccgcaa tgatttgaca cgttcgactt
     5701 attttagtgt ttatcgtgaa gattgtacca acgaatggtt cttggtttat ggagatgact
     5761 acaaggttca agacattatt gttgatagca aggcaggtcg catccttagt ggtgtatcct
     5821 tctgggatgc tccaactgca gaaaagattg tcagctttat cgacaaggct tatgtaagtg
     5881 gtgaatttgt tgatctctat tgggacaata tggttaagga taatatcaaa gagctagatg
     5941 tctatgttga agaattagaa ggcaatagca tttatgagat cgatagtgtc caagactatc
     6001 gtaaattaga agaaattctt aaaaacgaaa attaaagatt ccaacatctg acaaaatagt
     6061 cggatgtttt ttgatttttt acgaactttt acgaatagat agatgagtag aaaaagaaat
     6121 ggagttattt atgaaaatca caaactatga aatctataag ttaaaaaaat caggtttgac
     6181 caatcaacag attttgaaag tgctagaata cggtgaaaat gttgatcagg agcttttgtt
     6241 gggtgatatt gcagatatct caggttgccg taatccagcc gtttttatgg aacgttattt
     6301 tcagatagac gatgcgcatt tgtcgaaaga gtttcaaaaa tttccatctt tctctatttt
     6361 agatgactgt tatccttggg atttgagtga aatatatgat gcgcctgtac ttttatttta
     6421 caagggaaat cttgacctcc tgaaattccc gaaggtagcg gtcgtgggca gtcgtgcttg
     6481 tagcaaacag ggagctaagt cagttgaaaa agtcattcaa ggcttggaaa atgaactggt
     6541 tattgtcagt ggtctggcca agggcattga cacagcagct catatggcag ctcttcagaa
     6601 tggcggaaaa accattgcag tgattggaac aggactggat gtgttttatc ctaaagccaa
     6661 taaacgcttg caagactaca tcggcaatga ccatctggtt ctaagtgaat atggacctgg
     6721 tgaacaacct ctgaaatttc attttcctgc ccgtaatcgc atcattgctg gactttgtcg
     6781 tggtgtgatt gtagcagagg ctaagatgcg ttcaggtagt ctcattacgt gtgagcgagc
     6841 aatggaagaa ggacgcgatg tctttgctat tcctggtagc attttagatg gactatcaga
     6901 cggttgccat catttgattc aagaaggagc aaaattagtc accagtgggc aagatgttct
     6961 tgcggaattt gaattttaaa aatgacctaa gctagaattc taagaaaaaa tcaattttaa
     7021 gagaaaatga acccaacatt tccataataa aacgcatatt agcaagtttt taacacttga
     7081 taatatgcgt tttttctaag tggattagta gagtagagga tttttctcat ataatactct
     7141 tcgaaaatct cttcaaacta cgtcagcttc catctgcaac ctcaaaacag tattttgagc
     7201 tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaacct gtggctagct
     7261 tcctagtttg cgctttgatt ttcattgagt ataagggaaa gtatagtgaa ttgaaataag
     7321 atgtgaacaa ctctatcagg aaagtcaaat tgatttatag aaatatttta gcagccaagg
     7381 tgcactgtta tagattcaat acactataat ttagtgtaat tgagaaagga gaaatgattg
     7441 tgattgatgt tggctaggtt atgttcaatg attcctaccg tctcaaatct tgtcagtaag
     7501 gaaaaataaa ttcttcaaaa gtagagatta caaggcttgt ttaagaaaga attcaaagac
     7561 cttgacaaat aaaaataaaa tggttattat aaaaaatggt ctgaaataga tgatgatact
     7621 tttcgaaaat ctcttcaaac tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc
     7681 tacggttagc ttccgagttt gattttcatt tactagaaat gaaactgatg agagatatca
     7741 gtagacattt gagtcaggat attatggaaa atgataaaaa gagctcgtga gattggcata
     7801 tcagactact aaagtattga gtttgttagg attttagcga ctagttagct gggaaaggaa
     7861 gatatttgtg acaaataata aactgtattc gttgatagaa tttagaaata aaatatatga
     7921 agaattagaa ctttccagaa gtgatttagc gattttacta tgtgccatgc ttatcgcctc
     7981 tatcggatta aatatggatt cgactcccgt gattattgga gccatgttaa tctctccttt
     8041 gatgacacct attctgggag tggggctctc tctagctata tttgatttta aattgttaag
     8101 aaaatctttt aaaatattag ctattcaaat tcttgccagt ctaatagctt caacacttta
     8161 tttttatctt tctcccattt cgtatgctag ttcggagatt gttgctagaa cctctccgac
     8221 tatttgggat gttctcattg cttttgtagg agggatagca ggtattattg gtgctaggaa
     8281 aaaagagacc aataatattg ttcctggtgt tgctattgca accgccttga tgcctcctct
     8341 ttgtacagta ggttatgcta ttgcttctgc taatctaaaa tttatcatag gctcctctta
     8401 cctattcctc atcaattgta gctttattgt cattgcgact tatataggtg ttaggttgat
     8461 gatggttaag aaacattatt ttaaagataa tgaagaagac tctaaaatgc gtaggatttt
     8521 gcttctagtt gctgttttgc tgatgattcc gagtttcatc tctgcaacga ctttagtgag
     8581 agaaacgttg aaaaaagagt cccttaagaa atttatatca gagcagtttc aggggtataa
     8641 tattttgaaa aaaacctatt ctaaaaagac tcatacccta aagctaacca tttcaggaaa
     8701 ttatttgaca gaagaagaac tcgatatgat ttcaagtaag agaggtgact atggtttaag
     8761 tgatgtttct gttcaagttt cacaattgtc tgattcagaa caacttagta aggaagagct
     8821 ggtggagtat ttcttccagt atatcaagga taaggaagca aaagaaaagg agaaagccaa
     8881 taaatttgat acagagtctg aggagcaata atttcttgag aatagctggt ttctctcgtg
     8941 agtcttctat gtatatcaaa ggaagactga ggttttaagt ataaaacttt tcttctatta
     9001 tagtagctaa gtcttgacag ggttcaaaaa atggtttaca ctttataaag tttattactt
     9061 tgaaaaggtg tgatactgtg gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa
     9121 atctagtcat cgtggagtcg cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa
     9181 actacaaggt tttagccagt gtcgggcata tccgtgattt gaagaaatcc agtatgtccg
     9241 tcgatattga aaataattat gaaccgcaat atattaatat ccgaggaaaa ggccctctta
     9301 tcaatgactt gaaaaaagaa gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg
     9361 accgtgaagg agaagcgatt tcttggcatt tggcccatat tctcaacttg gatgaaaatg
     9421 atgccaaccg tgtggtcttc aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag
     9481 aacctcgtaa gatcgatatg gacttggtcg atgcccaaca agctcgtcgg atcttggatc
     9541 gcttggtagg gtattcgatt tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag
     9601 caggtcgcgt tcagtccatt gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg
     9661 ccttccagcc agaagaatac tggacagttg atgctgtctt taaaaaggga accaaacaat
     9721 ttcatgcttc cttctatgga gtagatggta aaaagatgaa actgaccagc aataacgaag
     9781 tcaaggaagt cttgtctcgt ctgacgagta aagacttttc agtagatcag gtggataaga
     9841 aagagcgcaa gcgcaatgct cctttaccct ataccacttc atctatgcag atggatgctg
     9901 ccaataaaat caatttccgt actcgaaaaa ccatgatggt tgcccaacag ctctatgaag
     9961 gaattaatat cggttctggt gttcaaggtt tgattaccta tatgcgtacc gattcgactc
    10021 gtatcagtcc tgtagcgcaa aatgaggcgg caagcttcat tacggatcgt tttggtagca
    10081 agtattctaa gcacggtagc aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg
    10141 ctattcgtcc gtcaagtgtc tttaatacac cagaaagcat cgctaagtat ctggacaagg
    10201 atcagcttaa gctatatacc cttatctgga atcgttttgt ggctagccag atgacagcgg
    10261 ccgtttttga taccatggct gttaaattgt ctcaaaaagg ggttcaattt gctgccaatg
    10321 gtagtcaggt taagtttgat ggttatcttg ccatttataa tgattctgac aagaataaga
    10381 tgttaccgga catggttgtt ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac
    10441 atttcaccca accgcctgcc cgttattctg aagcaacact gattaaaacc ttagaggaaa
    10501 atggggttgg acgtccatca acctatgcgc caaccattga aaccattcag aaacgttatt
    10561 atgttcgcct ggcagccaaa cgttttgaac cgacagagtt gggagaaatt gtcaataagc
    10621 tcatcgttga atatttccca gatatcgtaa acgtgacctt cacagctgaa atggaaggta
    10681 aactggatga tgtcgaagtt ggaaaagagc agtggcgacg ggtcattgat gccttttaca
    10741 aaccattctc taaagaagtt gccaaggctg aagaagaaat ggaaaaaatc cagattaagg
    10801 atgaaccagc tggatttgac tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc
    10861 gttttggtaa attctacgct tgtagcaatt tcccagattg ccgtcatacc caagcaatcg
    10921 tgaaagagat tggtgttgag tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa
    10981 ccaagcgtaa tcgcctcttc tatggttgca atcgctatcc agaatgtgaa tttacctctt
    11041 gggacaagcc tgttggtcgt gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag
    11101 tccgtggtgg tggcaagcag gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta
    11161 aataagagga ggggcccgaa agtaaatttc aggctccttt tggttagaac ttgacaaaat
    11221 acatcatttt atgcgaaact agaagaagat tattttaact aggagaagtt atgcgtctta
    11281 tctatctgat aattggtttt ttatcactgg ccttggctat tgttggggtt gttttaccct
    11341 tgttgcctac aacacctttc cttttgttgt ctattgcttg tttctccaga agttccaagc
    11401 gattcgaaga ctggctttat cataccaagc tctatcaaac atatgtagct gattttcgcg
    11461 agactaagtc tattacgcgt gaacgaaaga aaaaaatcat cgtatccatt tacattttga
    11521 tggggatttc tatttatttt gcccctctct taccagtcaa aatcggtctg ggtgctttga
    11581 ccatctttat cacttattat ctcttcaagg tcattccaga caaagaatag ttaaaacagt
    11641 agttatttac cttgataaaa ttgaaagcat attcataaca atatgatata ataaatttga
    11701 agtaatattc aaggagaatc aaatgattta cgaattttgt gctgaaaatg tgactttact
    11761 tgaaaaagcg atgcaggctg gagctcgtcg gattgaactc tgtgataatc tagcagttgg
    11821 tgggacaaca cctagctatg gagtgactaa ggcagcggtt gaactggcag ctaactacga
    11881 tacaaccatc atgaccatga ttcggccacg tggtggtgac tttgtctata atgacctaga
    11941 aattgctatc atgctagaag acatttgttt gactgctcag gctggaagtc aaggggttgt
    12001 atttggagct ttaactgctg ataaaaagtt ggataagcct aatctggaaa agttaattgc
    12061 tgcatcaaaa ggaatggaaa ttgtctttca catggccttt gatgaactaa gtgatgaaga
    12121 tcaaccggaa gctattgact ggctcagtca agccggtgtc actcgtatcc taactcgtgc
    12181 tggtgtgtct ggcgactcct tagaaaaacg ttttgttcac tatcacagaa ttttggagta
    12241 cgctaaaggt aaaattgaaa ttctaccagg tggggggatt gaccttgaca accgtcaaac
    12301 ctttattgac caggtggggg taacacaatt gcatggtact aaggttgttt tttaaaaaat
    12361 agaaaggaac tgctagcctt gggtagcagt tttcacttat gtttgaaatt tttaaatcct
    12421 atcaatttaa tcaagaaaag gctcatgatt atggttttat agaaaatagc gaagtctgga
    12481 catatagttg ccagattttg caaggtgact ttgtcatgac tgtgtccatc actgctgata
    12541 atgtgaactt tcaagtcttt gaccaagaga ctggtgacct ctatcctcac gttcatatgg
    12601 aaagcatgag gggaagtttt gtcggaaatg tccgtgaggc ttgtctggag attctttacc
    12661 agattcggaa ggcttgtttt gatgtgcaag attttatctg tcatcagact aagcgtatca
    12721 tgactcaagt tcaggaaaag tatggaaacc agttggagta tctgtgggaa aaatcgcctg
    12781 atacagctgt attgcgccat gaaggcaatc aaaagtggta tgccgtcttg atgaaaatct
    12841 cttggaataa gctggaaaag ggcagagaag gacaagtgga agcagtcaac ctcaagcatg
    12901 accaagtagc taatttgctt tcacaaaagg ggatttatcc agccttccat atgagcaagc
    12961 gctactggat tagtgtgtcc cttgatgata ctttatcaga tgaagaagta ctggaattga
    13021 tagaaaaaag ttggaactta acctctaaaa aatgaaatat tttaataatt ttcatgaact
    13081 ttcaattagc taaatattct ttactgaaga aatttttaga aaatatagga tttaccacac
    13141 tagaggaata tggtgccatc ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga
    13201 tcatctgttc gcctcactgt catgatgacc tcggaatggc agtggcaaat agccttgctg
    13261 ctgtcaagaa tggtgcagga cgtgttgaag ggactatcaa tggtattagg gagcgagctg
    13321 aaaatgctgc tttggaagaa attgcagtgg ctctcaatat tcgccaagat tactaccaag
    13381 tagaaaccag tattgtccta aatgagacca tcaatacgtc agaaatggtt tctcgcttct
    13441 ctggtattcc agttcctaaa aacaaagccg tcgttggtgg caataccttc tcccacgaat
    13501 ctggtattca ccaagatgga gtccttaaaa atcctctcac ttatgagatc atcacacctg
    13561 aattggttgg tgttaagatt ccgcttggaa aattatctgg tcgccatgct tttgttgaga
    13621 aactgagaga attggcccta gattttacag aagaggatat caaaccactc tttgctaagt
    13681 tcaaggcact ggccgataag aagtaagaaa tcacagatgc agatcctcga gctttggtag
    13741 ctggaaccat ggttgaaaat ccagaaggct tccactttga tgatttacaa cttcaaactc
    13801 atgcagataa tgacattgaa gcgctcgtta gcctagccaa tatggatggt gagaaagtcg
    13861 aatttaatgc gacagggcaa ggttccgttg aagcaatctt taacgctatc gataagttct
    13921 ttaaccaatc tgttcgtttg gtgtcctaca ctatcaatgc ggtaacagat ggaatcgatg
    13981 cccaggatcg ggttttggtc actgttgaaa acagagatac agaaaccatc tttaatgcag
    14041 cagggcttga ttttgatgtg ttgaaggctt ctgctattgc ctatataaac gctaatacct
    14101 ttgttcaaaa agagaatgca ggtgagatgg gacgcagtgt ttcttaccac gatatgccta
    14161 gtgtgtaaag gagaaggcta tggcaaagaa aatagtagct ctagtaggag acggaattgg
    14221 cccagaaatc atggaggctg gtttagaagt tctggaggct ctagctgaaa aaacaggttt
    14281 tgactatgag attgacagac gaccgttcgg aggtgcagat attgatgcag caggacctcc
    14341 cttacctgat gaaaccctta aggcaagtag ggaagcagat gctatcctac tagcagctat
    14401 cggtagtcct cagtatgatg gagcagcggt tcgccctgaa caaggcctga tggctctccg
    14461 taaggaactc aatctttacg ctaatattcg tcctgtaaaa atctttgaca gtctcaagta
    14521 tttgtcacca ctcaaaccgg aacgaatttc tggtgtagac tttgtcgtgg tgcgtgaatt
    14581 gacaggcgag atttactttg gagatcatat tcttgaagag cgcaaagcgc gtgatatcaa
    14641 cgactatagc tatgaggaag tggagcggat tattcgcaaa gcctttgaaa ttgcaagaaa
    14701 tcgcagaaaa atcgttacta gtatcgataa gcaaaatgtt ctagcgacct caaaactctg
    14761 gcggaaagta gctgaggaag tcgcacagga tttcccagat gtaaccttgg aacaccagct
    14821 ggtagactca gctgctatgc ttatgattac caatcctgct aagtttgatg ttattgtaac
    14881 ggagaatctt tttggagata ttttatctga tgaatcaagc gtcttatctg gtacacttga
    14941 ggttatgcca tcagccagtc attctgaaaa tggaccaagt ctctatgaac ctattcacgg
    15001 ttcagcacct gatattgcag gtcaaggaat tgccaatcct acttccatga ttttatcagt
    15061 tgccatgatg ttgagagata gtttcggacg ttatgaggat gcagagcgta tcaaacatgc
    15121 tgttgagaca agtctggcgg caggaatttt aacgagagat ataggaggtc aggcttcaac
    15181 aaaggaaatg acggaagcta ttattgcaag gttatgaagt tagacgaaaa aattactcta
    15241 gtccttttga tttggaatgt catcattttc ttgatttatg gcattgacaa attcaaggca
    15301 agaagaagaa cttggcgcat ccaagagaaa atcttactca ttttagccct tacttgtggt
    15361 ggttttgggg cctggttagc tggaatcact tttcaccaca agactcgaaa atggtacttt
    15421 aaaacagttt ggtttcttgg gatggtgacc acactagtag ccttatattt tatttggagg
    15481 taatggatgg cagggtcttc gagggaacac gctgcttggg ttctagcgga ctatggtttt
    15541 aaggtcgtga ttgcaggatc tttcggtgac attcattaca ataatgaact caataatggc
    15601 ctgttgccaa tcgttcagcc tagagaggtt agagagaaac tagcccagct aaaaccaacc
    15661 gaccaggtaa ctgtggactt ggaacaacaa aaaatcatct caccagttga agaattcacc
    15721 ttcgagatag atagcgagtg gaaacataaa ctcctaaata gtttggatga tatcggtatt
    15781 accttgcagt atgaagagtt gattgctgct tatgaaaaac aacgaccagc ctactggcag
    15841 gattagaaaa aatagaaaag gaaatatagt aaactgaaat aagatgtaaa caaatgaatt
    15901 agagcttaac atccatttcc agcaattttt tagaaactac agtggactat tctggattca
    15961 acacattata aaattatgac aaaacacatt cacaagaagg ctacgacatt ttaaaaggtg
    16021 agggcggatg tatcgtttgc cctactaaag ttggttacat tatcatgacc agcaacaagg
    16081 caggacttga gcgtaagttc gcagccaaag aacgtaagcg taacaaacca ggtgttgttc
    16141 tctgcggtag catggatgaa cttcgcgctt tagcgcaact caacccagaa attgaagcat
    16201 tctactaaaa acattggatg aagatattct tcttggttgt atccttcctt ggaaaccaga
    16261 agcctttgaa aaactcaaag catacgggaa tggccgtgaa gaacttatga ctgatgtacg
    16321 tggtactagc tgttttgtta tcaagtttgg aaaagcaggt gaacaattgg ctgccaagct
    16381 ttgggaagaa ggtaaaatgg tctacgcctc atatgcttca atgacaaaac gattgaaact
    16441 cgctatgaga gcaaggtgta atggtgtcta tggtcgataa ggacggcaaa ctcatcccag
    16501 aacaaggagg agcacgttca acttcaccag caccagttgt gatccgtaaa gggcttgaca
    16561 ttgataaaat catgatgcac ctgtcagata cttttaactc atgggactac cgtcaggttg
    16621 agtattatta agataaaaaa agaagtctag tgttatgaga tattaaagct cctaacactg
    16681 gactttttgt ttagaatttc ttttcttttt ctataggata tggtattcta tgtagaaaat
    16741 atatgttaat aagtaatgcc aatatttaaa catcattagt aaaaggagtt agattgatga
    16801 ataaaagaaa agttagttta gaagattttt ataaatggta tagtctaaat aaagaagagt
    16861 tattaaataa ggcaactgtt ggtgaaaagt ttaatgataa attaaaagaa gagtttctcc
    16921 aggaatggcc tttggatagg attttaacaa tgtcaatcga tgaatatgta ataggaaagg
    16981 gacagcaaaa taagtcttta tgctacgctc ttgagaaggg aaaatacaaa aatctatttc
    17041 ttggaatttc tggtggctca gcttcaaaat ttggtattta ttggaataaa aaaacaaaca
    17101 aatataaaga tcaagctaat aatgagattt cagagttgga tcagcgattt tcaaaattaa
    17161 aatcagattt gtatgaaatt atca
//
LOCUS       DAVLFB010000036        16993 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLFB010000036 DAVLFB010000000
VERSION     DAVLFB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.036"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            321..1367
                     /gene="pheS"
                     /locus_tag="VXD58_001084"
     CDS             321..1367
                     /gene="pheS"
                     /locus_tag="VXD58_001084"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV0675798.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1367..1876
                     /locus_tag="VXD58_001085"
     CDS             1367..1876
                     /locus_tag="VXD58_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675799.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1953..4355
                     /locus_tag="VXD58_001086"
     CDS             1953..4355
                     /locus_tag="VXD58_001086"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV0675800.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVCAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4423..5424)
                     /locus_tag="VXD58_001087"
     CDS             complement(4423..5424)
                     /locus_tag="VXD58_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV0675801.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5580..6026
                     /locus_tag="VXD58_001088"
                     /pseudo
     CDS             5580..6026
                     /locus_tag="VXD58_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6152..6418
                     /locus_tag="VXD58_001089"
     CDS             6152..6418
                     /locus_tag="VXD58_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675802.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6679..8928
                     /gene="metE"
                     /locus_tag="VXD58_001090"
     CDS             6679..8928
                     /gene="metE"
                     /locus_tag="VXD58_001090"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV0675803.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDATFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRRDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8992..9858
                     /gene="metF"
                     /locus_tag="VXD58_001091"
     CDS             8992..9858
                     /gene="metF"
                     /locus_tag="VXD58_001091"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV0675804.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10543..12756
                     /gene="pnp"
                     /locus_tag="VXD58_001092"
     CDS             10543..12756
                     /gene="pnp"
                     /locus_tag="VXD58_001092"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV0675805.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            12772..13389
                     /gene="cysE"
                     /locus_tag="VXD58_001093"
     CDS             12772..13389
                     /gene="cysE"
                     /locus_tag="VXD58_001093"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV0675806.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13401..14285
                     /locus_tag="VXD58_001094"
     CDS             13401..14285
                     /locus_tag="VXD58_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675807.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14372..15715
                     /locus_tag="VXD58_001095"
     CDS             14372..15715
                     /locus_tag="VXD58_001095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV0675808.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGMKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15708..16094
                     /locus_tag="VXD58_001096"
     CDS             15708..16094
                     /locus_tag="VXD58_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV0675809.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16098..16982
                     /locus_tag="VXD58_001097"
     CDS             16098..16982
                     /locus_tag="VXD58_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV0675810.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
BASE COUNT         5049 a         3317 c         3891 g         4736 t
ORIGIN      
        1 tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga taggtcttgc
       61 aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga aagactagta
      121 gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat atgaaagctg
      181 ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga tgaaaacgga
      241 tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct ttttatctac
      301 gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg cttcgcgaag
      361 aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg caagatttgc
      421 gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg atgaaagatg
      481 tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt gatgtcttga
      541 cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg gctcaactgg
      601 ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt caccgtcacg
      661 ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt tatcaagtcg
      721 tggatggttt tgaagtggag caagactact ataactttga acgtatgaac cttccaaaag
      781 accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc ttgctccgta
      841 cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct aaaggtcctt
      901 tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg acccacagtc
      961 accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg gctgatcttc
     1021 aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa attcgtttgc
     1081 gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct tgcttcaagt
     1141 gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt atgggagccg
     1201 gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta tactctggtt
     1261 ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc aacgatatcc
     1321 gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga ttagaaaagt
     1381 agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc gtgaaacctt
     1441 tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt atagtctgaa
     1501 aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta tgcatgagga
     1561 ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga gagaattaga
     1621 ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat ttggactagg
     1681 taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt cttgggcttg
     1741 gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat atggttttga
     1801 aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt ggttactgag
     1861 aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg aaaagaacca
     1921 gtgtggcaat ggtcattctg gagaactaaa ttatgcttat atcttataaa tggttaaaag
     1981 aattggtgga cattgatgtg ccatcacaag agttggctga aaaaatgtca actacaggaa
     2041 tcgaggtaga gggtgtcgaa tcaccagctg ctggtctctc aaaaattgtc gtcggtgagg
     2101 tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt aacgttggcg
     2161 aagaagagcg tcagatcgtt tgtggtgccc caaatgtgtg tgctgggatc aaggtcatgg
     2221 tggctcttcc aggagctcgt atcgctgata actacaaaat caaaaaagga aaaatccgtg
     2281 gtttggagtc acttggaatg atctgttcac ttggtgaatt gggaatttct gactcagttg
     2341 tgcctaagga attcgcagat ggcatccaaa tcttgcctga agatgccgtg ccaggtgagg
     2401 aagtcttttc ttacctagac ttggatgatg aaatcatcga actttccatc acaccaaacc
     2461 gtgcagatgc cctttctatg tgtggagtgg ctcacgaagt ggcagccatc tatgacaagg
     2521 cagtcaactt taaagaattt actctaacag aaactaatga agctgcggca gatgcccttt
     2581 ctgtcagcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg gacaatgtga
     2641 ccatcgcacc aagtccacaa tggttgcaaa atcttctcat gaacgaagga atccgtccca
     2701 tcaataacgt agtggacgtg accaactaca tcctgctcta ttttggtcaa ccaatgcatg
     2761 cctttgactt ggataacttt gaagggactg acatccgtgt gcgtgaagcg cgtgctggtg
     2821 aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac ctagtcatca
     2881 ctgtcgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca acagaaatct
     2941 ctgaaaaatc tagtcgtgtt gtccttgaag ctgctgtttt caatggcaaa tctatccgta
     3001 agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa aaaggaatta
     3061 atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg gaacttgcag
     3121 gtgcgacggt gcgtaagggc atcgtttcag cgggtgagct tgatacttca gatgtagaag
     3181 tttcttcaac ccttgctgat gttaatcgtg tcctcggaac tgagctgtct tatgctgatg
     3241 tagaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac agctttacag
     3301 tcagagtccc acgtcgtcgt tgggatatca caatcgaagc tgacctcttt gaagaaattg
     3361 ctcgtatcta tggttatgac cgcttgccaa ctagtctacc aaaagacgat ggtacagcag
     3421 gtgaattgac agccacacaa aaactccgcc gtcaagttcg tactattgct gaaggagcag
     3481 gtttgacaga aatcatcacc tatactctaa caactcctga aaaagcagtt gagtttacgg
     3541 ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt tcagtcctcc
     3601 gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct cgtaagaata
     3661 aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat ccaaaagaag
     3721 aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt gctgaaaaag
     3781 atttccaaac agcagcagtt ccagttgatt tcttctatgc taagggaatc cttgaagccc
     3841 tatttactcg tttgggactc caagtaacct atacagcaac atctgaaatc gctagccttc
     3901 atccaggtcg tacagccgtg atttcattcg gtgaccaagt tcttggtttc cttggccaag
     3961 tgcatccagt cactgccaag gcttacgata ttccagaaac gtatgtggct gagcttaacc
     4021 tttcagctat cgaagctgcg cttcagccag cgactccatt tgtagaaatc accaaattcc
     4081 cggcagtcag ccgtgacgtt gcccttctcc tcaaggcaga agtgactcat caagaagttg
     4141 tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc tttgacgtct
     4201 tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg accttccaaa
     4261 atccagaaga tagcttaacg gacgaagaag tcgcacgcta tatggaaaaa atccaagcat
     4321 cgctcgaaga aaaagtcaat gcagaagtgc gttaaacgat agtatgatac aaattccccc
     4381 tctagtttat gaacactaga ggggtgattt tatataaaat aattaaaatg tgatttctgt
     4441 aaaaatacat ctatgatcag agttttgaaa acttacaatg tctaaatctt taacatagta
     4501 gtggttttta ggcaataaaa tatgatctat tgttgcatta aaaagttttg gactttggct
     4561 attccaagtt cctctttcaa aaggtggcag tacatttaat gcgtccctat gagagcttat
     4621 ttttgcaagt gctccatgac gcatagttgc attaaaatca cctgcaataa tagcctttgg
     4681 atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga tttccattaa
     4741 acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt gttttctcga
     4801 atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg tatagaaacc
     4861 ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag gtggagaagt
     4921 gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt tgattctctg
     4981 gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca tatcggcgtc
     5041 aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga cgttccaagt
     5101 tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat ctgttccgaa
     5161 taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta tttgtttatt
     5221 tttcttgtaa aaagataggg aaaaaaatac gaaacttaaa atagcagcca tagttgcagt
     5281 agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc aaatgttaag
     5341 attagaagat gggaaaaaag aataagttat aatagggata gaaaagagaa tgaccgaaag
     5401 ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg ttgctcggag
     5461 ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc ctcatagatt
     5521 aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa ggcacagaaa
     5581 tgcaagacaa ttatactaca aaaggcaaac atttgacaat cgatagccgt cgcttaatcg
     5641 aacgatggaa aaaagaagga aaatcaaata gagaaattgc ctctctactt ggaaaagctc
     5701 ctcaaactat ccatactgaa atcaagtatg ggacagtccg acaatgtctt ggaaaagggc
     5761 gcttcaaaga gatttattct gccgactacg ctcaacagtc ttatgaaaat aatcgcaagc
     5821 actcggtcaa gaaatcaagc ttgactaaga aactaaagga aaagattctc cactatcata
     5881 accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg ggaatttcaa
     5941 ccatttacta ttggattcat cgtggaaaat tggggttaag caagcaggat ctgctttatc
     6001 ctagaaaagg aaaagcgctt aagaaatagg ctagcaccaa ctttaaaccc gctggtcaat
     6061 ctatcgaatc ataattcgct cattttttta aaaatacata gacgtaaaaa aaagagggtt
     6121 aagtgatgaa gaatagtgct attgggagta attggaagga tgtccatctg agctctttag
     6181 caaggaggaa atccttgaaa gtgatatgcg agtggctatc atgagcgagt tgattgaggc
     6241 taggtatgaa caaggaatca gtcagaaaaa gctggaagaa gtcagtggaa taagccagcc
     6301 tgtcatagct aggatggaga caggagagag cagtcttcag ttggatacgg tcttaaaagt
     6361 tctaaccagt ctaggaaaga cactagcagt cgttccactt gaacagggga aaagttgact
     6421 agaatcagtt atagtctttt ctaataacag gtcatagtca cttataagaa ttactaataa
     6481 catgttttag tattctaatt ggaatatagc tgtaaagtaa gaattacaaa ggatttgaga
     6541 cgtttgcttc aagtcttttt cttttgtccg aaacagatat atagtttcaa actatatcaa
     6601 accctaattt tttacaatta tacagacgct ttcttttctg ttaagatagt ttcaacaaca
     6661 aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt gggcgaattc
     6721 cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga agaagaactt
     6781 ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga aaaaggcatc
     6841 actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc gactttcctt
     6901 ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga gcgctacttc
     6961 gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc aatgaagaaa
     7021 tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac tcaagtaaaa
     7081 ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg attgaacact
     7141 cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga agaaggcgtg
     7201 aaagcagacg acttcgtaga tagcttagtg gttgcttacc aagaagtttt tgctaaattg
     7261 gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa agacttgaca
     7321 gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga caaaaaaggt
     7381 cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc tgaccttgta
     7441 aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac tcttgaactc
     7501 gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa tggtaaaaac
     7561 atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc agctgaaaac
     7621 atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc taatgaagaa
     7681 tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga tgaaatccgt
     7741 gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa caaagaactc
     7801 tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc tggcttgaca
     7861 gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca agaagaagct
     7921 ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac aaaagaagtt
     7981 cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta cgatgctttc
     8041 cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt tgatgtcctt
     8101 gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa cttgtcagga
     8161 tacctcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt aaaaccacca
     8221 atcatctggg gtgatgtcac tcgtcttaac tctatcactg ttaaatggtc tagctatgca
     8281 caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac catccttaac
     8341 tggtcattcc cacgtgaaga catctctatc aaggattcaa ctcttcaaat cgcccttgct
     8401 atcaaggatg aagtacttga ccttgaagct gctggtgtga aaatcatcca aatcgacgag
     8461 gctgctcttc gtgaaaaatt gccactccgc cgtagggact ggtacgaaga ctaccttgac
     8521 tgggctattc ctgccttccg cttggtacac tcaacagtag cgccagacac acaaatccac
     8581 actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa catggatgca
     8641 gacgttattt cctttgaagc tagccgttca aaccttgaaa tcttggacga actcaaagcg
     8701 aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc tcgtgtacca
     8761 aatgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc aagcaagaaa
     8821 gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac aaaagaaagc
     8881 ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga ttggatttct
     8941 ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca tatgtcacgc
     9001 caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg taatggtaac
     9061 attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag tgtaactgcc
     9121 agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt tatccaaaat
     9181 gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa ggacaaggtt
     9241 gctgaaacta ttgctgactt ggacaaagtt ggggtgcaga aaatcttggc tcttcgtggg
     9301 gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga cttgattgag
     9361 tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc agaagggcat
     9421 ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt ggatgcaggc
     9481 tgttcgagcc tcgtaactca gctcttcttt gataatgagc gcttctatga ttttcaggac
     9541 aagtgtattt tggctgggat tgatgttccc attcatgcag gaattatgcc aattctgaat
     9601 cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc acgcaaattt
     9661 aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc aggacttgcc
     9721 tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt gcatctctat
     9781 acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc cttgtttaat
     9841 caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga atggttcctt
     9901 tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc tgtacgtact
     9961 tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga tttaacgaca
    10021 cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta gctaatcgcc
    10081 cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct aatgatgtat
    10141 cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct aggagcagtc
    10201 gctccgtcgt tcgatttcct attttccttt gagttgcttg acggcttggt atcttgatga
    10261 cttgaatttt ggtctcattt cttaggaaaa aagataagct tcctagcact cgtcgagtgc
    10321 gacgtcacct tcctattttt ctacagaaat gttcggcaag ccgaaccgtc caaaatatct
    10381 tgtgtaattg aacacggccg aaaagctgtg taaaaaagat aaactgtctt gtcttcatcg
    10441 aagacttcgt cagtttccta tttttacttt gcttttgacg tccttggtat cttgatcttt
    10501 gtaggcaagg cgtataattt catcaatcca aaggggatta aaatggcaaa acaagtgttt
    10561 caaacgactt ttgcgggtcg tgagttaatt gtagaaactg gtcaggttgc taagcaagca
    10621 aatggctctg ttgtcgtacg ttacggtgag tcaactgtct tgactgctgc cgttatgtct
    10681 aagaaaatgg caactgggga tttcttccca ctccaagtca actacgaaga aaaaatgtat
    10741 gcggctggga agtttcctgg tggctttatg aaacgtgaag gacgtccttc aacagatgcg
    10801 accttgacag cgcgtttgat tgaccgtccg attcgtccta tgtttgcgga aggtttccgt
    10861 aatgaagtcc aagtcatcaa tacagtgctt tcttatgatg aaaatgcgtc tgcaccaatg
    10921 gctgctatgt ttggttcatc tttggcactg tctatttcag atattccatt tgacggacca
    10981 attgctgggg tacaagtggg atatgtagat ggccaaatca tcatcaaccc aagtcaagaa
    11041 caagcagagc aatctcttct tgaattgaca gtagctggaa ccaagcacgc tatcaacatg
    11101 gtagagtctg gtgctaaaga attgtcagaa gaaatcatgt tggaagcgct ccttaaaggg
    11161 cacgaagctg tcaaagaatt gattgccttc caagaagaaa tcgttgctgc tgtcggtaaa
    11221 gaaaaagcag aagtggaatt gcttcacgtg gatgctgaat tgcaagctga aatcattgca
    11281 gcctacaaca gtgacctcca aaaggcagtt caagtagaag agaaattggc ccgtgaagct
    11341 gcgactcaag tagtgaaaga ccaagtgact gccgtttacg aagaaaaata tgcggaccac
    11401 gaagaatttg accgtattat gcgtgatgtg gctgaaatct tggaacaaat ggaacacgca
    11461 gaagtgcgac gtttaattac agaagacaag gtgcgtcctg atggtcgtaa ggtcgatgaa
    11521 atccgtcctt tggatgcggt tgtcgacttc cttcctcgtg tgcacggttc aggtctcttc
    11581 actcgtggac aaacgcaagc cctttctgtc ttgaccttgg ctccgatggg agaaactcaa
    11641 atcattgatg gtttggatct ggagtacaag aaacgcttta tgcaccacta taacttccct
    11701 caatattctg taggggaaac aggtcgttac ggtgcgccag gtcgtcgtga aatcggtcac
    11761 ggtgcccttg gtgagcgtgc tcttgctcaa gtcttgccaa gcttggaaga attcccatac
    11821 gctatccgtc tagtagcaga agttttggaa tcaaacggtt cttcatctca agcttctatc
    11881 tgtgcgggaa ctcttgccct tatggctggt ggtgtgccaa tcaaggcgcc agtagctggt
    11941 attgctatgg gacttatctc agatggaaat aactacacag tattgacaga tatccaaggt
    12001 ttggaagatc actttggaga tatggacttc aaggttgcag gtactcgtga tgggattaca
    12061 gcccttcaaa tggatatcaa gattcaaggg attactgcag aaatcttgac ggaggctctt
    12121 gctcaagcca agaaagcgcg ttttgaaatc cttgatgtca ttgaagcaac cattccagaa
    12181 gttcgtccag aattggctcc aactgctccg aaaattgata cgatcaagat tgatgtggac
    12241 aagattaaga ttgtcatcgg taagggtgga gaaaccatcg acaagattat cgctgaaaca
    12301 ggtgttaaga ttgatataga cgaagaagga aatgtgtcta tctactctag tgaccaagat
    12361 gctattaacc gtgccaaaga aattattgct ggtttggttc gtgaagccaa agtggatgaa
    12421 gtttaccgtg ctaaagtcgt tcgtatcgag aaatttggtg cctttgttaa cctctttgat
    12481 aagacagatg cccttgttca tatctctgag atggcttgga ctcgtaccaa tcgtgtagag
    12541 gatttggtag aaatcgggga tgaagttgat gttaaggtta tcaaaattga tgaaaaaggc
    12601 cgtatcgatg cctctatgaa ggctcttcta cctcgtccgc caaaacctga gcatgatgaa
    12661 aaaggtgaaa agtctgagcg ccctcaccgc ccacgtcatc aaaaggatca caaacctaag
    12721 aaagaattta cagaaacacc aaaagattca gaataagaaa aggagaaatg tatggggtgg
    12781 tggcgcgaaa ccattgatat tgtaaaagaa aatgatccag cggcccgcac cactttggag
    12841 gttttgctga cttatccagg tgtcaaggcc ttggcggccc accgtctctc gcattttctc
    12901 tggaagcacg gcttcaaact attagctcgt atgtacagtc agttttggcg cttttggact
    12961 cagattgaga ttcatccagg agcccagatt gattcaggtg tttttattga ccatggttct
    13021 ggtctggtga ttggagagac agcgattgtt gaaaaaggcg ttcttctcta tcacggagtg
    13081 actctcgggg gaacagggaa agactgtggc aaacgccatc cgactgtacg aaagggagcc
    13141 ctcatatcag cccatgccca agttatcggg cctgtggaaa ttggtgaaaa tgccaaggtc
    13201 ggtgctgcag cagttgtcgt agcagatgta cctagtgatg tgacggttgt cggtattccg
    13261 gccaagattg tccgtcttca tggtaagaaa gatgagcctg ttattcacga agtcgaagaa
    13321 aaacgggagt attatgtcaa taaactcgag caggctaaag atgccagtca cagatcgtct
    13381 ggtttgtaga ggatacctat atgattcaag caagaaacaa gttaagccaa gaggagctat
    13441 ctgaggcgaa aaaagtaatt aactgttgcc aaaactatga cggtacctat cgcgatccct
    13501 atctctctaa catgcttaat tttgacccaa acatgcccgc ctttttcctt tattatgaaa
    13561 aaggcaaact tgttggttta ttaactgtct atgcagatga ccaagatgta gaagtgacga
    13621 tattggttca tccaggtcat cgccgtcagg ggattgcgcg tgcattgttt actagttttg
    13681 agagagaaac agcttctttc cccattcggt cagtcacttt tcagacagaa cgtatttttt
    13741 tagagaatca tcctgacttt gccagtaact ggggactaat cgaggatgaa gagacagaaa
    13801 cttggttagg taaggataga agaccttatc cgttagcaaa tgtttcccat cttgaagttt
    13861 tgttagcaga tagttcgtat caggatcaaa ttagtcaatt aaaatttcag gcattttcag
    13921 aggaacatga atcgagagaa gttgtggata gatatgtcgc tgaagctctg aaggatccag
    13981 aaagtcgctt atatattttg ttaaaagacg gtcaggttat tgggacttgc acggtagatt
    14041 tatcgactaa tacgaattac ttctacggtt tagcaatatc ggaacctgaa cgtgggaaag
    14101 gctatggaag ctacttagca aaatccctcg tcaaccaact aattgagcaa aatgacaagg
    14161 aatttcagat tgcagtggaa gatagcaatg taggtgccaa acgtttgtat gaaaaaattg
    14221 gctttgtcaa acagacacag gtggtttatc tgaatgagaa aggagcaagg gattccgaag
    14281 tgtagagaca ttcggactga aatttacttg agctcttagt ttagtgatga aactaacagt
    14341 tcttggattt tagctttcct gattatgatt tatgattaaa atctatgaca ccatgtctcg
    14401 tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg tttgtgggcc
    14461 aaccgtgtac aactatattc acgtgggaaa cgcccgttcg acggtagctt ttgatacgat
    14521 tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt ttacagatgt
    14581 ggatgataag attatcaacc gtgccaggga agaaggcatc acacctcagg aggttgcgga
    14641 taagtacatc gctgcctttc gtgaggatgt gacggcctta ggcatgaaac ctgcgactcg
    14701 ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag acttgatcga
    14761 gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa aatcccacaa
    14821 ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt caggtcgtac
    14881 cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga aatcttccaa
    14941 accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt ggcatattga
    15001 gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg gtggtggagc
    15061 tgacctagag tttccacacc acaccaatga aattgcccaa tctgaagcta aaacaggcaa
    15121 ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg tcaaaatgtc
    15181 taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg atgggcaagt
    15241 acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca cggaaaaggc
    15301 agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac aaccatttac
    15361 aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag cagctatgga
    15421 tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca aatggatcaa
    15481 ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag aaatttttgg
    15541 aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc aaaaacgcca
    15601 agaggcgcgt gccaatcgtg actttgagac agcagaccaa atccgtgacc aattggttac
    15661 tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg attgatgtca
    15721 atctcattaa cgggattgcg ctagcctttg agggagatgc ggtgtattct atgtatattc
    15781 gccgccacct catcctcaaa ggtatgacca agcccaataa actccaccaa gaggcgacca
    15841 agtacgtgtc agccaaggct caggctcgtc tgattgctct catgttggag gagcagatcc
    15901 taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagt cacacaaagg
    15961 ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagca gttatgggct
    16021 atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg tgcatccaaa
    16081 aagtggaggg ctagaacatg agggcaaaag aactacaaga ctggtttcct gaggctcgga
    16141 tttcagacca accagtagag aaagagggct atctcacgct tcctttagct tctcagcagt
    16201 ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt gcccttttga
    16261 cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt gagggcaagg
    16321 gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt tcttattttc
    16381 agcaggaaaa tctgacttct tggctagata tgatgcggac tctttttccg aattgtcaga
    16441 cagtgctaca ggtcggagct caggattatg ttttcgtgct tcaacaagac aaatacactt
    16501 ctgtaagaga tattttgagt gatacgattg aagcggttga gtatgacttt ggacttcgtc
    16561 tttctatcat gttgggtcag gtttggtctc agacaggaca tcaagcccta tcagacttaa
    16621 tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa ggtgttcata
    16681 ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg aagccaatta
    16741 aagaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc attctctctc
    16801 tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg caccgcaatt
    16861 ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg aaagagttga
    16921 ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata gttggtttgt
    16981 aaagaagtta ata
//
LOCUS       DAVLFB010000037        16876 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLFB010000037 DAVLFB010000000
VERSION     DAVLFB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.037"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            92..274
                     /locus_tag="VXD58_001098"
     CDS             92..274
                     /locus_tag="VXD58_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV0675811.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            219..930
                     /locus_tag="VXD58_001099"
                     /pseudo
     CDS             219..930
                     /locus_tag="VXD58_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1000..1362)
                     /locus_tag="VXD58_001100"
     CDS             complement(1000..1362)
                     /locus_tag="VXD58_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675812.1"
                     /translation="MPKTKTLAELADVILWGFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1740..3620)
                     /locus_tag="VXD58_001101"
     CDS             complement(1740..3620)
                     /locus_tag="VXD58_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV0675813.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVMGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3614..4483)
                     /locus_tag="VXD58_001102"
     CDS             complement(3614..4483)
                     /locus_tag="VXD58_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0675814.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLYRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4567..7212)
                     /locus_tag="VXD58_001103"
     CDS             complement(4567..7212)
                     /locus_tag="VXD58_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV0675815.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7403..8683)
                     /locus_tag="VXD58_001104"
     CDS             complement(7403..8683)
                     /locus_tag="VXD58_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV0675816.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCDVNDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8854..10938
                     /locus_tag="VXD58_001105"
     CDS             8854..10938
                     /locus_tag="VXD58_001105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV0675817.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGEDSLFHRQSSYNRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKILVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10978..12657)
                     /locus_tag="VXD58_001106"
     CDS             complement(10978..12657)
                     /locus_tag="VXD58_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV0675818.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQQVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLYFYLNLTVDGQLVDQLQVQVS"
     gene            13413..14642
                     /gene="arcA"
                     /locus_tag="VXD58_001107"
     CDS             13413..14642
                     /gene="arcA"
                     /locus_tag="VXD58_001107"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV0675819.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14700..15716
                     /gene="argF"
                     /locus_tag="VXD58_001108"
     CDS             14700..15716
                     /gene="argF"
                     /locus_tag="VXD58_001108"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV0675820.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15881..16828
                     /gene="arcC"
                     /locus_tag="VXD58_001109"
     CDS             15881..16828
                     /gene="arcC"
                     /locus_tag="VXD58_001109"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV0675821.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4848 a         3844 c         3422 g         4762 t
ORIGIN      
        1 ctgagtaaaa actctcgaaa ttcaattcgc tatattatca ctcaacttga acaaatacca
       61 gaaccacaaa aaacagatgt aaaggaaaaa tatggactta aaatttgaag gggttgattt
      121 agaatacaaa aaagctaaaa acaatcttcc agagtctttc tgggaaacct actcagcatt
      181 tgcaaataca aatggcggga aaataattct cggtatagat gaaaaaaaca ttgacaccta
      241 ccaaagagtc aatagactcc ctgcgaaatt atgaagcaag taaacaatta actgatacac
      301 gatttaagcg tcttgttggt gttcagcgta ccacttttaa agagatgtta gctgtgttaa
      361 aaacagatta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta agcctagaag
      421 accttcttat ggccactctt caatatgtgc gagaataccg cacttatgaa gaaattgcgg
      481 ctgattttgg tattcacgaa agcaacttaa tccgtcggag ccaatgggtt gaagtaactc
      541 ttgttcaaag tggtgttacg atttcaagaa ctcctctcag ttctgaggac acggtaatga
      601 ttgatgcgac ggaagtacaa atcaatcgcc ctaaaaaaac aattagcgaa tcattctggt
      661 aaaatcttgg ctgacagtga ttatcaaggg ctcatgaaga tatatcctca agcacaaact
      721 ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaagcctg taaccatgcg
      781 ctatctaagg agagaagcaa ggttgagaac atctttgcca aagtaaaaac gtttaaaatg
      841 ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa tttgagtgct
      901 ggtattatca atcatgaatt aggattctag ttttgcagga agtctattga aatactaagc
      961 ctacacagac tgattccaat tgttttttag atttcaattt taccattctt catagtcaaa
     1021 accatcataa tctggatatt gttctggcga cgttccaacc gaacgtacga gataagcttc
     1081 ttcgtcctct gtctcgattt ctctcagtac ttggcattca aaacgccatt catcaccgaa
     1141 gtcgaaaata aacttaaatt tttgtttgac acttaggcta tccagataga cattttctgt
     1201 gtaacgttct tcaacatcgt cactaacaaa gctaagaaag taagaatctg catgactcca
     1261 ctcaacatta tccatgaaaa atgcgtgagc atgatcattt gcaaaatcaa aaccccataa
     1321 aatcacatct gctagttccg ctaacgtttt tgtttttgga atctatatat gtcggtagca
     1381 acctttttta aatttgacgg aaatgatcca agcttctaca gatgctggct tttcagcttc
     1441 tatttcttgt tttactaatt ttaagtgtag cataatacct ccaattgctc atcgatagaa
     1501 ttgctcttag gaaacattca ctttctgcta ttttatcata agtttgtcta actgtagcat
     1561 ttgaaaccag ataattcttc tgtttcacgc gaaacaccct gcgccaagca aggtgttctt
     1621 gtttttatac tcaatgaaaa tcaaagagca aactaggaag ctagctgcag gctgctcaaa
     1681 acactgtttt gaggttgtag atggaagctg acgtggtttg aagagatttt cgaagagtat
     1741 taagtcgtat aaatcgtcga agctgtcgca atggtatgcc actggttagc tgtagttgct
     1801 gcgaaatcct tgtctgcgta gaagtcagta aatggtagga tttcaacttc cagctcgtcg
     1861 atgcggtcaa gctggccagc cagataaacc tcgatacggc tttctgctcg tttgatgcgt
     1921 tgcaagagtc cgcccatacg gatgtcaact gtatccaaac caaagacctt gttttctttc
     1981 aaccattggt gactaaagag ggcatggaag tcttcaattt ggcttctaag ttctggtaat
     2041 tcttgtctgg cgatttgttg taaactttct ttatcatccg cttggtaggc ctgacgaatg
     2101 cgtcgtccca catctacttt gctacttaaa atagcattca actgggcctg agtttcaaag
     2161 agataagcat agtttccagc tttttcttta atgtcagcaa tagttcccgc cgcctgagcg
     2221 aagtgtggct tgtcctgttc aggtgtcatg tgtcggtcaa gtatcggaca gagaacatcc
     2281 tgataaaaga catagcggtt gggattgatg ccactgagat tacctggtag gtctggtaag
     2341 aggttggcaa ggtcaatctg cataaaatcc tcaaccgtta gaccagtatt ggtcttgaaa
     2401 tgcgcagaca aactatctag gtcattgcga tagctgagtt ctgcccagat ttgcaagctt
     2461 ggtaggatag agaactgggc agtttcacca ccattgtctc cccaacccat tacgatgact
     2521 tctttaatct gattggcacg gcaggcttta ttagcctcga tagccactag acggctaaaa
     2581 tggttgtgag gtgtaaagcc aatccacttc caagctcccc ctgcaaatgc aaggtcatgg
     2641 ctaatcttgt gatgattgcg gaaattacgg ttgtattttt cctcgctatc ctgataataa
     2701 tcccagtaaa ccagagtcac acggtctttg agacggtcta ggtagacacg agtttcctct
     2761 ggaatttcca catcacgatc gtactggcca tccgctgaca tgagtttgaa gaacatatca
     2821 ctccacatct ggcagtggaa accatatttg tcagcaatat ccagcacgcg ctccaagtgt
     2881 tggcacatga ggagactacg atccacaaca ccgttcaaaa tcaggtagcg tcccaaacca
     2941 accaagtggg cttcgtccat cccgatattg accttgcgag tcttcagttt agacaacgtg
     3001 gcaaacatgc catcaatcag gtcataaacc ttttcttcgc caatgagaag aatgtcctct
     3061 acatcacgga gctcctgcac ttccttgaca ccccatttga caaaggccga caagtgggcc
     3121 aaggtctgga tgcatggtac aaaggtcatg tcaaactgtt gagcataggc ttcgatttcc
     3181 tgcaactcct ctgctgaata agctccacgg aaatagccaa agtaaggctg tccttcaatc
     3241 tggtaagtgt cttccatgta aagctcaaag gttgagtagc ccatgagagc caagacctca
     3301 atcatctgct tggcagaagc cacattcagc accgcatttc gagaacagtc aaccatgtaa
     3361 gccaaatctt cgtaagctgc ttgttcctca atctctactt tatcagcttc tgctagaact
     3421 gttaccaaca aggataaggc acgataaagt tggtgaggtt tgcggtaggt caattgatag
     3481 tgaccttcct caccctcgat agagatagag gcttggtcag actgagtgac agccacttcc
     3541 acatctggta gagaaatgtg accttttaaa acctcaatag cttgcgtttg tttgggacta
     3601 agtcctgtaa atcttaccat tgcttttcct cctgtagcca gttgacaagg gcaccgtaga
     3661 gattggcatc tgcgtgatag gtgcaggcct ggataactgg tgcgaccgtg tattcttcgt
     3721 aggcatcgac aaagtcttca acagccttct taacaccttg gataaaatct ggattttgac
     3781 tgatagagcc acccagactg atgacatctg gatcgatcag atactggata ttgagcaagc
     3841 cttgcgtcag attgcggttc atgcgctcaa tggcttcttg acaaagagca ttaccagctg
     3901 cggcctcttg gtaaatcttg cgaccgtccc aatcagtatg accagatttt tcaatcacgt
     3961 atcgtaccat attcccagtt gatgctagtt gcgaccagtt attaagtttt tcagcagggg
     4021 caagggttgt catgtagcca aattctccac ccagaccgtg gcgacctcga taaagtctac
     4081 cattgataat catggctccg ccaatccctg tcccaatcac gacacaggct gcattttcaa
     4141 gctctggatg agctagtagt tcactgagtc caacgcagtt ggcatcattt tctaaatgga
     4201 caggtagctg ataagagcta agcgcctcat accaagaaaa gccatggatg tagggcaccg
     4261 cactgaagcc atcaatcaca cctgtctctt gattgaccgc acctggaacg ctcatagcaa
     4321 tcccactgta atcctgttct gacaagcgtt gatctagcca cgctagtaaa tcctccaagt
     4381 tttcaggcgt tgaaatactt gtcttatcca gtattttccc atcaggagtc agactggcaa
     4441 acttaatccc agtccctccg atatcaatcg ttgcaatggt cattgttttt accataaaaa
     4501 ggggcgaatc agcagttagt tcttactttt tcgcccctcc tttcttttga tgtttacttt
     4561 ttgaaattaa atttcttctt ttttgatgaa ttctgtacga atctcttgtg gagccaatag
     4621 tcctgaatga actggatatg gtcgctcaag taagtcaaga atggtttgtt gatgttctga
     4681 tatgcgcaca ttttcttgac tcatattata gtaacggagg acatagcctt cttcattttc
     4741 cgccacttta aaggctgttg gacaaacttg cggtatgctg agaacagaat ggctcaagag
     4801 gctaccagtc gcaaccacgc ttccttcctg tctagcaagc tgaaggctgg taaacggtgt
     4861 ctgcaaggct ttggcacgac gataggctga gaagcgttct tgggcttggt ggcattcaat
     4921 cgcaaattcg acttcaaact cacgcaagca ttgtgcttct ggcgttggga agtagcccca
     4981 gtcacctagc tcacctgatg cacgcaaaat ggtcacggca atggtgttat ccccaaggat
     5041 ttcgtattca ttcaatccct tgttggatac agtcacccct ttttcatcgt catacagact
     5101 gacaaaagct tgttggtgtt gaggattttc aggattttcc catgaagcag ctggtttgtt
     5161 tggtcgtgtc accacctcat agatactttc agaatcattg cttggacgcg tgttatgagt
     5221 cttgaccaag agacggatac ggtgatcctt ggcagtgtta gtaaagcgag tcttgaagcg
     5281 gatttgtgga ttgtcaacaa agacaatcaa ctcagtttcc agaggaatgt ttgtcaattc
     5341 ttctgaccgt ccagcctcac gcttcataaa ctcgatgata ccttgttgct cttcttctag
     5401 cttttcatcc gcactgacag gcacggtcaa ttcatgtttg agcaagattt tagcatagcg
     5461 atctgtgttt tccaagacct cgtggccctt aagctctgca aagattggct ctgttccttt
     5521 tggttggaaa tagatatact cgtttccgat gtcaccccgg tcttcgaagc ggataaaatc
     5581 ttcataggct tcgtgagttg tcttatcata gactgtgatg ttgtcatcca cactcaccgt
     5641 tacgaatggt gtatcaatca ctccgttttg gtaaataccc tcacggtgtt cttgttctcc
     5701 ttccagcaat tggaaggttg tccaagaaag cggtgctagg tgaactggaa tggtcacgcg
     5761 cacttgtcga gcaatacgag cttggcggaa cttatctttt ggtaaatcgt attcaaaatt
     5821 agctccgagg tcttcgattg taacctctac aggacgacca tccaagtcct ccacacggta
     5881 acttggcaag ataagagcag ccatcttttt gtagccttct gttgggtgca attccttgaa
     5941 atcacaagtc gccacatcaa tcactgtgct gacagtatcg accttatcat gcaagcctgt
     6001 gttaatgaca gtaaagagat agtcactttg agccttatcc gtagcaattt tacccttcca
     6061 ctcgttgagc aaattacttt taacaaagtt tcctacttgg ttgaccttgg caaaacgcgt
     6121 ttccatctcg cggtgaactt cgtccacgct acagccacag atactatcat gtggcgcatt
     6181 ctgcaaaagt gttttccaag cataggtcaa ctggtccttg tggttgtgtc caccagtgat
     6241 aatagtcaag ggttctacca cttgctctag gaggttgcta ttttcttgga aggcttgttt
     6301 taggtaaatg cgggatgaag aagtgttggc aagtgtgtac cagccatctg tttcctgact
     6361 ggtcaactcg cctgtaacag ttgataagtg ttcaggaagc gcaccttcta cagcttgaac
     6421 atattcatca aaagaactat gaacaaagat tacatccggg aagagttcat ttgccacacg
     6481 aatggcttcg ctcagatttt tctgtacagg ctggtggtca cagccgttca tcatcaacca
     6541 ttggttggtc gaagcgtagg cacgcacatc tgacaatttt tgtttccaga aggtcaaggc
     6601 ctcatctttg tcaactggga tttcattccc attactgtac cagttggcaa agagaatacc
     6661 taaaacacga ctaccatcca caccctgcca gtacatttct gaaaactggg atgtaaactg
     6721 ctcatcttca aggacttggt tgtcaaatcc aatcggcttc acaccacgac caaaggccgc
     6781 cacgtgaatg cctgattttt gaagaatttg aggcgcttgt cccatatttc caaaggtatc
     6841 tggaaagtag ccaatctggg ttgatttacc ccatttggca gcttcttgtt gaccaatcaa
     6901 ggtattgcgg acattggctt cactggagat caagtagtca tcctgcaaga tgtaaaaggg
     6961 accaatttta agtttgccct cgtcaatgta gcgttggacc ttgtcgcgat tttcagggcg
     7021 aatttgtaag tagtcatcaa ggacaatagt ttgtccatcc aagtggaaac tcttgaactc
     7081 agggtcattt tcaaagagat caaagagatt gtcaaacaat tccaccaact gcatacgatg
     7141 gctttcaaaa ggcaagtacc actcacggtc ccagtgacta tgtgagataa tatgtacaac
     7201 aacattttcc atgaagtaaa acctcattct aaatttaaat tttaacgttt taaatgtaaa
     7261 cttgtgattc tgataagaat cggttgagct aatactcaac gaaaatcaaa gtgcaaacta
     7321 ggaagctagc cgcaggttgc tcaaagcact gctttgaggt tgtagataaa gctgacgttg
     7381 tttgagagat tttcgaagag tattagcgaa tatccaagta atccaagacc aactcacaga
     7441 acatcatgtt agcccaggag aaccattcac gagagtagag ggtcggatca tctacgtgga
     7501 agctttcgtg catgacacct gtaccaccat cgcaggcaac cagctgatcc agcaagaatt
     7561 ttttctctgc cttatctctt gttgtcaatc cttggataga aagggcgatt ggccagatat
     7621 agcgatagaa ggtatgagaa cttccgagac cgctagcgta ttctccttgg tagaagtatg
     7681 gattttcaga gctcaaaatg gtacgacgag tagcttgata cacttcatcg ttgacatcgc
     7741 agtagcccag atagggcgca gctagtagac ttggtacatt tggatcatcc atgatgctgg
     7801 catttcctag gccatccact tcaaaagcgt aaatcttttc gcccttgctg ttggtggtgt
     7861 aagcgtagtt ttcgattcct tcttggattt catcctgaag acgcttggca tcagcaataa
     7921 cactctggct atcagctagg tttaatgctg cgaagatttc ttgcacataa cccaagacta
     7981 ctacagcaaa catatttgac ggaatcaagt aactatactg gcaacagtca tcactcggac
     8041 gaaaggctga ccaagtcata cctgtcactg caaagtcagg tccaaagcca tcatttacca
     8101 aggtgtcttc cttacggtcc gtatctcgga caaaacgata aggagagttc ttgtggtctt
     8161 gttcaactgt ccatagatgg agaatttcct tagtcgctac gacaaaaatc tcatcaaact
     8221 gactagtctc gccagtctct ttccagagga gataagccaa ctgcaaagga tagcaaagtg
     8281 aatccacctc atacttgcgc tcccagatcc agccgttaag gtctgtgtga tcagtctcgt
     8341 ggtgcccttt ccagttctcc tcaatgttga aggagttagc atagggatcc ttgagtacca
     8401 aggtcatctg acgtttgacc aaacctgcaa tggtctgacg caggagggca tctcttttag
     8461 ctacatgaag gtagggtctg agttgggctg tcgaatcccg aagccacatg gcaggaatat
     8521 ccccagtcaa gacaaaagtt gaaccatctt ctaagatttc aaccgtattg tccaaggtat
     8581 cggtgtagca acgctcgaaa acatccaccc actctggatg gtccttagcc cgctctgcta
     8641 cttcatctag ccattctcta acaatttctt tcgaataaat catcgtgcaa ttccctcttt
     8701 caaacaagtt tcatgttcag tatatagctt ttgagacaga aaatacatac acaaatgata
     8761 gtcatttttc tacaaaatag ttatttttga aactcctttt ctaaaggccc tctttttctg
     8821 atataatgta gaaaaacagc taaaggaaag actatgaaac cactacttga aaccatcgat
     8881 acccgctttg gaactaccaa caagcatgcc ttttctcggg gaaataccct gccatacaca
     8941 ggcgtgcctt ttgggatgaa ttattttgtg ccccagacca gtgaccagga cggagcttgg
     9001 ttcttcgatc cgcatctgcc tatctttcag gggattcgat taactcacca gcccagtcct
     9061 tggattgggg actactcttg gctccttctg acacctgtca ccagtcaact gggtgaagac
     9121 agtctctttc accgccagtc ttcctataat agggataagg cctctttcca acctcattat
     9181 ctaaagattt tctccctgcg ctatcagatt gaaacccagc tcacaccgac ttgctacggt
     9241 gcttctattc gtttgaagca aaagcaaggc aaagccctct ccctctatct tcacgcaaca
     9301 gatgaactga cagtagagca agtagataag cgaactcttg ccctgcgaca agaaggtaaa
     9361 actgaaacta acaaaaattc gctaacgatg ttcactgccc tgcaaatgaa cacggatatt
     9421 cttgctatca gccaagaagc tggagactgg cgaattgact tagtaagtag tcaaaccgag
     9481 atgcaactag ccacttcttt catctctcct tctcaagctc taatcaatct acctcaagaa
     9541 gattttgata gctgtaaatc aagtgcccaa gcggattggg aaaatctcct ccatcgtttt
     9601 gacattatag agacaggaga ggctgaccga accttctttg accactgcct ctacagactc
     9661 ttcctcttcc cacagacttt ttatgaggtt gatgagtcag ggcaagccat ccacatggat
     9721 ctggctactg gtactgtcaa gcctggtgtc ctctttagca acaatggttt ctgggatacc
     9781 ttccgcacca ccttccccct ctttgccctt atcataccgg agcactatca acgcttttta
     9841 gaaggtttcc tcaatagcta ccgcgatact ggtttccttc caaaatggct ggctccagat
     9901 gaacggggta tgatgccagg tacactttta gacggtatta tcgcagatag cgcctgcaag
     9961 gacatggccc ctgacctaga aggagaactc ttccaagcca tgctcgaaac agccagcaaa
    10021 gccgaccctc tcggcatcaa tggccgccac ggactagccc aataccaaga actaggttac
    10081 ctctctaccg accaccatga aagtgttagc catactctag actatgccta tagtgacttt
    10141 tgtatcgcca gctgtgccaa aaaactagag aacatagaaa tcgctgaaac ctacaaggct
    10201 gcttcacaaa attaccgcca gctatttgac gctgagacag gttacatgcg agcacgagac
    10261 aatcaaggaa actttcaccc tgacttctct ccttatagtt gggggcgaga ctacgctgaa
    10321 tgctctgcca ttcaagctac tttaggcgtt ctccacgaca tccctggctt aatccaactg
    10381 atgggtggaa aagaaacctt tagcaactat cttttgaaag cctgtcaaga tgctcccctc
    10441 tttgaaacaa caggctatgg ttacgaaatt cacgaaatga gcgagatggc tactgctcct
    10501 tttggacaaa tagccatttc caaccagcct agtttccaca ttccttatct cttccgctac
    10561 agcgattacc ctgactacac tgcccttctt atcaagacgc tccgtcagaa agcttttcac
    10621 ccaagttggg aagcctatcc tggagatgaa gacaatggta gtctctctgc ttggtacatc
    10681 tggtcagctc tcggatttta tccaacctgt ccaggaaaac caagctatga cctcggaatc
    10741 cctctctttg accatctacg agtctaccta gctaaagaag ataaatggct ggatatccat
    10801 actaaacaaa accacaacca ttttaacttt gtcaaagaat gccgactgga caaaatactc
    10861 gtatcaacta ttcaacacca agacctctta aaagctgaac aactaacttt caccctcagc
    10921 tggttaccaa gtcactaata accaaaagaa cctttgcatc gcgcaaaggt tcttctttta
    10981 tgaaacttgg acttgaagtt gatccacaag ctgaccatcc accgttaggt tcagatagaa
    11041 atacaaggtt ttatctcctg cgaaatacaa ggttggtagc gtcagacttt tttcagtctc
    11101 accagcttga aacgcaagga cttgaatctc atcctgatag gcgacaccat ggacacctgt
    11161 ccccggttga atcgaaatct tagcttctaa aggaccacta cattctctgc gcttaactgt
    11221 aaaataggca ttttctccct ttgccacagc taggcttttt tctgcaaatg ctagttcctg
    11281 aacaacttct ttttttgata atccaggagt tttataaagg gaaatcttgg tcaatacagg
    11341 aatagattgt gcctcggtaa tcatcacgcg caccttctgt gcctctacta gcggacctcg
    11401 caagaggcgc ttatgaccaa ctgtgaagcc cctgccaaat tcctgccaga caccatccac
    11461 ctctacttgc acatgaaaag cagcgattcg ttgccctagc ttcaaatctt ctcttaactc
    11521 aattacatca aaagttttag gagaacctaa gtcgagttct aactggatgg gcaagtctgc
    11581 atcgtttgcc caagagctgg tctcaaggcc gtctgtcaaa tggcgacaag caaagtctgc
    11641 ggaaagagct ggaccagata cctcagctcc cagagccaaa tcttctttat agagctcatt
    11701 gcgataggtc gcaaattcat aaagtcgttc aatatccttt gcatcaaaga gcccagcttg
    11761 attcggcgga atattaagca agagtggagt tcctcgccct actgagtgaa agtagatttc
    11821 gaccaactcc tcgagagact taggatcctg atcctcatgg tagaaccagc ctggacggat
    11881 ggaaacatct gcctctccga ttgaaaaaat cgtgcccgag ggatccccgt gctgaagata
    11941 gtccagctct gcttctgttc ctagtttatc aggattcacc ttttgccaca gtggatcacc
    12001 tgcataccct cgttcattgc caatccagcg gatactggtg ccttctgttg aaaaaatcaa
    12061 acaatcgccc tgcaagtcac gaatggtttc aaaccatttt tcaaattcat aattgacctg
    12121 ctgggcccct tcccctctag caccatccat ccaaacctca gcgaacttac cagcattccc
    12181 atagttagga tttgataaga tttccttcaa ctgagccaga taataggcat tgtagtccgc
    12241 ttctcggtcc acatgataga ggggactatg ggcatcccac ggtgacaggt agacccccat
    12301 atccatatca aactctgtgg cagcttggga tacttcaagg agcaagtcgc cctttcctct
    12361 cctccaagga ctgaccttaa ccgaataatc tgtgtgagct gtcggataaa ggacaaagcc
    12421 atcgtggtgc ttgaccacca aaatcaactt tttgaagccc gtttccttga gcacacgaac
    12481 ccactcacgc gcatccaact gactcgggtt aaagcgctca ggatcctcct gacctgttcc
    12541 ccattcctga ttataaaaag tattaggtcc aaaatggata aaggctgcta gttcatctcc
    12601 cagataagct agctgagtct gacttggtaa cggtccatgc ggtttgattt tcttcatcgt
    12661 tatatatctc cttcttttgc aacttctctt gttcacaagg ttatccacag gttgtggata
    12721 ataaaccatg atctaacagc cagctctttc caactccaac tggtaggctc aggatttttc
    12781 tcctcaatat tccctcttca aacagctctg tcatatggtt ctgctaacac ctctcagaat
    12841 gctactctcc ataattattc tcaggcttct ttcaggtcat tgtgtgaaga ccattcactc
    12901 tccagacttt tccacagatt gtgaataagt gattattcta aactatcacc tcgtcgacta
    12961 taacaaaaaa taaaaagagg gtcaataaga taattatctc ttcctaaata ctcagaaaag
    13021 attctataat ttgtacaatt gaacaagaaa tgcttctcca aaatctatct tcaaaagaat
    13081 gttgcatctt tttatccttt cacacaaaca tttctcttta tacagatcca agtgataacc
    13141 agatgataat tggacacttt ttggttattt gtgtattttt tgaaaaattt tttaaaaaaa
    13201 ttttatatcc tctttaatag aagtttatgc atttgtatat atgtattcca tcctatgttg
    13261 tgtataataa cataaaattg tgctaaatat cataatttat attattttcc gagaaattat
    13321 agccattgac attacctagg aattgcgtta taacatacat gtaagcggta cccaaaatgg
    13381 tgccaagtca aaatttttaa ggaggaaaat acatgtcttc acatccaatt caggtcttct
    13441 cagaaattgg gaaactgaaa aaagttatgt tgcaccgtcc aggcaaggag ttagaaaact
    13501 tgttgccgga ctatcttgaa aggcttcttt ttgatgatat tcctttcttg gaagatgctc
    13561 aaaaagaaca tgatgcattt gcccaagctc ttcgcgatga aggaattgag gttctctacc
    13621 tagaacaact cgctgctgaa tcattgacct ctccagaaat ccgcgatcaa tttatcgagg
    13681 aatacttaga cgaagccaac atccgtgatc gtcaaactaa ggttgctatt cgtgaattgc
    13741 ttcacggcat caaggacaac caagaattgg ttgaaaaaac aatggctggg attcaaaaag
    13801 ttgaattgcc agaaattcct gacgaagcta aagatctaac tgacttagtt gaatcagatt
    13861 atccatttgc aattgacccg atgccaaacc tctatttcac tcgcgaccca tttgcaacaa
    13921 ttggaaacgc cgtatcgctt aaccacatgt ttgcagacac tcgtaaccgt gaaacactct
    13981 acggtaagta tatcttcaaa taccacccaa tctatggcgg aaaagtggat ttggtctaca
    14041 accgtgaaga agatacgcgt atcgaaggtg gagacgagct agttctttct aaagacgtcc
    14101 ttgcagtagg tatctctcaa cgtacagacg cagcttctat cgaaaaactt ttggtcaaca
    14161 tcttcaagaa aaatgttggc ttcaagaaag ttttggcctt tgaatttgct aacaaccgta
    14221 aattcatgca cttggatact gtcttcacta tggtagacta tgacaagttc actattcacc
    14281 cagaaatcga aggcgacctt cacgtttact cagttactta cgaaaacgaa aaacttaaaa
    14341 ttgttgaaga gaaaggtgac ttagctgaac ttcttgctca aaaccttggt gtagaaaaag
    14401 ttcatttgat tcgttgcggt ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg
    14461 acggttctaa cactttgacc atcgcacctg gtgtggtagt tgtttatgac cgcaataccg
    14521 tgaccaataa gattttggaa gaatacgggc ttcgcttgat taagattcgc ggaagtgaat
    14581 tggttcgggg ccgtggtgga cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt
    14641 aatcgctgtt cggtattcgt caatagaaaa tgtaaaaaat agaaagagga aataataaaa
    14701 tgacaaattc agtattccaa ggacgcagct tcttagcaga aaaagacttt acccgtgcag
    14761 agttagaata ccttattggt ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc
    14821 aacaccacta ccttgctggc aagaatatcg ctctcctatt tgaaaaaaca tctactcgta
    14881 ctcgtgcagc ctttacaact gcggctatcg accttggtgc tcacccagaa tacctcggag
    14941 caaatgatat tcagttgggt aaaaaagaat ctactgaaga tactgctaaa gtattgggac
    15001 gtatgtttga cgggattgaa ttccgcggat tcagccaacg tatggttgaa gaattggcag
    15061 aattctcagg cgttccagta tggaacggtc taactgacga atggcaccca actcaaatgc
    15121 tcgctgacta cttgactgtt caagaaaact tcggtcgctt ggaaggcttg acattggtat
    15181 actgtggtga tggacgtaac aatgttgcca acagcttgct cgtaacaggt gctatccttg
    15241 gtgtcaatgt tcacatcttc tcaccaaaag aactcttccc agaaaaagaa atcgttgaat
    15301 tggcagaagg atttgctaaa gaaagtggcg cacatgttct catcactgaa gatgctgatg
    15361 aagcagttaa agatgcagac gttctttaca cagacgtttg ggtatcaatg ggtgaagaag
    15421 acaaattcgc agaacgtgta gctcttctta aaccttacca agtcaatatg gacttagtta
    15481 aaaaagcagg caatgaaaac ttgatcttcc tacactgctt gccagcattc cacgatactc
    15541 acactgttta tggtaaagac gttgctgaaa aatttggtgt agaagaaatg gaagtaacag
    15601 acgaagtctt ccgcagcaag tacgctcgcc acttcgatca agcagaaaac cgtatgcaca
    15661 ctatcaaagc tgttatggct gctacacttg gtaaccttta tattcctaaa gtataatttt
    15721 agataataaa ccgtctacca acagctatga gggctgcgac taatagcttt agtccggtcc
    15781 tcttttatat aatggtaatc tattatttct tataaaatat gtgaaaaatc attaaattga
    15841 aatctaaacg cattctattg agtgtgataa aggagaattt atggcaaatc gtaaaattgt
    15901 agtagctttg ggaggaaatg cgattctttc ttctgaccca tcagcaaagg ctcaacaaga
    15961 agctttagtt gaaacagcta agcatcttgt aaaattgatt aaaaatggag atgatctgat
    16021 tatcactcac ggtaatggac ctcaagttgg gaatctcttg ctccaacatt tggcatcaga
    16081 ctctgaaaag aaccctgcct tcccactcga ctcacttgtc gctatgacag aaggtagcat
    16141 cggtttctgg ttgaaaaatg ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa
    16201 tgttgcctct gttgtaacgc aagttgtcgt agataaaaat gatccagctt ttgttaactt
    16261 gagtaaacca atcggtcctt tctattcaga agaagaagca aaagcagaag ccgaaaagag
    16321 cggagcgact ttcaaggaag atgctggccg tggctggcgt aaggtcgttg cctcaccaaa
    16381 acctgttgac atcaaagaaa ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt
    16441 cgtagctgca ggtggtggcg gtattcccgt cgtcaaagaa aacaatggac atttgactgg
    16501 tgtcgaagcg gttattgata aagacttcgc ttcccaacgt ttggcagaat tggttgatgc
    16561 agacctcttc atcgttttga caggtgtaga ttatgtattt gttaactaca acaagccaaa
    16621 ccaggaaaaa ttggaacatg tgaatgttgc ccagctggaa gaatatatca aacaagatca
    16681 gtttgcacca ggtagcatgc ttccaaaagt agaagcagct atcgcttttg tcaatggtcg
    16741 tccagaagga aaagcagtta ttacttccct tgaaaatcta ggcgccttga ttgaatctga
    16801 aagcggaaca attattgaaa aaggataagt tgttttacta ataagatgta ttctatttct
    16861 agtatcttta tatcaa
//
LOCUS       DAVLFB010000038        16741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLFB010000038 DAVLFB010000000
VERSION     DAVLFB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.038"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(20..217)
                     /locus_tag="VXD58_001110"
     CDS             complement(20..217)
                     /locus_tag="VXD58_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0675822.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(318..926)
                     /locus_tag="VXD58_001111"
     CDS             complement(318..926)
                     /locus_tag="VXD58_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV0675823.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(951..1463)
                     /locus_tag="VXD58_001112"
     CDS             complement(951..1463)
                     /locus_tag="VXD58_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV0675824.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(1549..2484)
                     /locus_tag="VXD58_001113"
     CDS             complement(1549..2484)
                     /locus_tag="VXD58_001113"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV0675825.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMYKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(2555..2854)
                     /locus_tag="VXD58_001114"
     CDS             complement(2555..2854)
                     /locus_tag="VXD58_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV0675826.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(2844..3593)
                     /locus_tag="VXD58_001115"
     CDS             complement(2844..3593)
                     /locus_tag="VXD58_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV0675827.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(3647..4006)
                     /locus_tag="VXD58_001116"
     CDS             complement(3647..4006)
                     /locus_tag="VXD58_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV0675828.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(4008..5408)
                     /locus_tag="VXD58_001117"
     CDS             complement(4008..5408)
                     /locus_tag="VXD58_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV0675829.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(5703..5942)
                     /locus_tag="VXD58_001118"
     CDS             complement(5703..5942)
                     /locus_tag="VXD58_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV0675830.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(5974..6444)
                     /gene="ssbA"
                     /locus_tag="VXD58_001119"
     CDS             complement(5974..6444)
                     /gene="ssbA"
                     /locus_tag="VXD58_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV0675831.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(6456..6746)
                     /locus_tag="VXD58_001120"
     CDS             complement(6456..6746)
                     /locus_tag="VXD58_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV0675832.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(6899..8245)
                     /gene="asnS"
                     /locus_tag="VXD58_001121"
     CDS             complement(6899..8245)
                     /gene="asnS"
                     /locus_tag="VXD58_001121"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV0675833.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(8261..8626)
                     /locus_tag="VXD58_001122"
     CDS             complement(8261..8626)
                     /locus_tag="VXD58_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0675834.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(8619..9806)
                     /locus_tag="VXD58_001123"
     CDS             complement(8619..9806)
                     /locus_tag="VXD58_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0675835.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(9803..10291)
                     /locus_tag="VXD58_001124"
     CDS             complement(9803..10291)
                     /locus_tag="VXD58_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV0675836.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            10612..11160
                     /locus_tag="VXD58_001125"
     CDS             10612..11160
                     /locus_tag="VXD58_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV0675837.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(11397..16720)
                     /locus_tag="VXD58_001126"
                     /pseudo
     CDS             complement(11397..16720)
                     /locus_tag="VXD58_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT         4433 a         3786 c         3092 g         5430 t
ORIGIN      
        1 tgaaaccctt gattttttcc tattctttag taaaaacgtt tttcaaacct tcaacggcac
       61 cttctacagc accttttgcg tcttctacaa cgtcttttgc cttagcaact gttttttcaa
      121 cagctccttc aagttctgtc ttgctgtctc cagtaacttt accaaaacct tctttgacag
      181 cgcctgttgc ttgttccaat ttgttttcaa gtgacatatg attgtctcct ttagtttatt
      241 ccgatatgtg ttaccggtta catatagttt ataccgaata ctaaggaaag tcaaacaatg
      301 tactcagaaa caagaaatca cgctaagtag aaagttaatc tctccttatc aaaatcgata
      361 gccaactcat actttccatc ttcattttgc acaatatagc ctaagacaac caaactgtcc
      421 acaaagatat cacggcattt ctgcataagc tggtcttttt tgagaaattt caacaaaaaa
      481 gccgtcatat acttgagtgc atactcagga ttaacatctc ctaaaatgtc atagagctcc
      541 tgctgttttt ctgtcaaagg atactgctgt ttgaccttgt aaaaataatt ggacagagtc
      601 attttaatgc gcgcaaagtc cgtcttttca actaaaatag ctgcattgat ttgattgcgc
      661 aattccgtct caaaactctg ctccaacaag gcttgataga ccggactagc ttctctgaca
      721 aaaatctctt gatccagttc aagactatca agtgattcaa gcgtaggaaa attgaggtaa
      781 tagcgcttat tttcacgtag aatcaagcct gcctttatat actcttccat gagtttatca
      841 actgcaacat ctggaaattg agccttaatt tcccgtagaa tcacatcctc atactggtcc
      901 agatagcgga tcaattctcc aaaaatggct gtctcgtcaa acgagatgga ttaaaaatct
      961 gaatcatgaa gattcctttc tttacattgt aacagaggcg atgctgccaa ctgactagga
     1021 ttggtaccag cttggttcag aggaacaggc agacctagtt ctatgtaata ctccctccaa
     1081 aaatcactaa tctcctgctc cccatctcca aatttcatag gaatagtttc aaaaataggt
     1141 aggatttctg ctatatactg ggagcagtag aaaccaggtg catctggata gaaagaagca
     1201 ttgtagggtg ctccaagatg tttgcaagct ctttccttca ccgactggat atccatttct
     1261 gggtaaacat agaggtcgta taaatgattg gactcaaaga agtctgccgg ttcttgacag
     1321 acaacaccag cctgtccact agcatgataa atcatcccat ccaaataaat ggcaacatgg
     1381 ctatagttac ctgtggaagt ctggatggcc tgtcccatgt ctgacccatc tctcacaaaa
     1441 atcaaatcgc cattttctaa catacttcac tcctagaaaa aaggagccga aactcctttc
     1501 gatataaggc aaactagact ttccgatttt gagctgacac ccaaaatctt acgcattaaa
     1561 gctttcagtc aattgaggta ccacttgttt cttacgtgaa acggcaccag caaggaaggc
     1621 atgattgttt tcaagtttga agttgaaagc cgcttcgacc ttgtacatat tggcaccaag
     1681 agccaagatt tctgagtttg agttgacgat atctgtaatc atcaagacaa agtcagagta
     1741 gccgtttgat tcgttggcag cttgcattgc agcttcaatt tctgcttggc gttccaaaac
     1801 ttcagcgatg tcaactgtgt tcacttgggc aacacggaca ttatttccgt tgagttcaaa
     1861 agtcttagca tcgatatcaa tcaattcttc agcagatttg ctagccaagt tggtaccagc
     1921 tttcaacatt gccaaaccat attcttccaa gttcacacca gccaattcag ccaattcagg
     1981 agcaatgatt ttatctgttg ggtgtgttgt tggtgatttc aaaagaaggg tatctgaaat
     2041 caaacctgaa agcatcaaac ctgcaatctc tttaggcaca gctacaccat gttctttgaa
     2101 catacggtaa acgattgaag acgctgatcc aactggctcc aaacgcatgt aaagtgggct
     2161 tgcagtttca aagttagcca cacggtggtg gtctacaaca ccgtaaactt ctacttcagc
     2221 gatatctgat acagattgtt ggaattcatt gtggtcagtc aagataactt gctctgcccc
     2281 ctctgctttg gcagaagtga taacacgtgg tgcttccaca ccaaaatagt tcaagacaaa
     2341 ggctgtttct tcatttggag ttccaagggc aacagcttcc gtatccaaac cgtaagcttc
     2401 ttttgcaagg taggcaaaag ctacagatga tccgatggca tctgagtctg gattttggtg
     2461 accaaatact agaatcttgg acatgataat acctcatttt gtttattttc tttattgtag
     2521 catttttttc gctttttgac aaaggtaaga ggagtcaaag gactccccac gattcttcaa
     2581 aataactgag taaatttcta tcttgatttt cagataaaaa ttcttccttc tgtggcctct
     2641 tcttacgctt gagaagagct tcagccgaca tggcttcttc cttactggca aaaccttgag
     2701 catagataag tttgactggc aagcgtgctc gtgtatattt ggctcccttc ccactattgt
     2761 ggatagcgag gcgtcttctc atatcagtcg tatagcctat atagtaggat ccatcacgac
     2821 actccagaac gtacatataa gccttatgat ccataataaa tctcttcgat ttcgggcgta
     2881 taagagccat catcattgtg gacaatcaaa ggaggtaaga ccttaaagcc acttgttgag
     2941 ccatccttga tcgcctcaat caaaagcata ttggcttcct tttctctttt tggataaaca
     3001 aactgcaggc gcttaggggc tagattatgt cgttttaacg tatccaaaat atccagaagt
     3061 cgatcaggac gatgaaccat ggccaaacgc ccattagact tgagaatact ctgggcacta
     3121 cgacagattt cttccaaatt agtcgtgatt tcgtgtcgag ccaagagata atgttcactc
     3181 tcgttcagat tagaataagg attcaccttg aaatagggtg gattacacaa aatcatatcc
     3241 accttactcc cctgaatgtg agcaggcata tttttcaaat catcgcagat gacctgcatt
     3301 tgctcctcta atccattcaa acggacagag cgttcagcca tatccgccaa acgctcctga
     3361 atctcaacag acaatatctg tgcttgagta cgagtgctag caaaaagccc cactgctcca
     3421 ttcccagcac agaaatccac aatcaacccc ttcttaggaa aacgtggaaa tcgtgataag
     3481 agaacactat ccaccgaata gctaaaaacc tctctatttt gaatgatttt gatatctgtc
     3541 gaaaagagct ggttaatgcg ctctcctgat tttaataatt gttcttcttc catggtccta
     3601 ttatagcaaa ttcatattaa cattacaaaa aatataaaac tctaaactac ttcttctttt
     3661 ttaaatggtg cagggcttct ccagtccaga ttggtagcat tcgtcgaaag ggagcaaaac
     3721 cgtagttaaa gcggtcgctt gaaaagcgtc tccgtctagg aaactggtac ttttcttcct
     3781 ccaaagtgcg gatagaaaga ctggctttcc ccgtaaattc atctaaatcc actacctgaa
     3841 cttgaacctc ttcatcgact ttcaaggttt catgaatatt ttcaataaat cctgtccgaa
     3901 tttctgaaat gtgaatcagc cccgtatcac ccgtctctaa ctcaacaaag gcaccgtagg
     3961 gctgaatccc tgtaatacgc ccctttagct tatcaccgat tttcatctta gtcctcgatt
     4021 tcaatagttt caattacaac atcttcaact ggcttgtcca tagctcctgt ctcaacagca
     4081 gcaatggcat ccaagacagc gtaagatgct tcatcagcta actgaccaaa aaccgtgtga
     4141 cggcggtcta ggtgaggtgt cccaccttga ttggcataga tttctgcaat cggttctggc
     4201 caaccaccac gagtaatttc tttcttcgaa taaggtaggt gttggttttg cacgataaag
     4261 aactggctgc cgttggtatt tggaccagca tttgccatgg aaagagcacc acggatattg
     4321 taaagctctt ctgagaattc atcctcaaaa gattcgccgt agattgactc gccacccata
     4381 ccagttccag ttgggtctcc accttggatc ataaagtcct tgataatacg gtggaaaatg
     4441 acaccatcat agtagccatc ttttgaaaga gatacaaagt tagccactgt tttaggagca
     4501 tgttcaggga aaagcttgat acgtaagtct ccgtgattgg tcttaatagt cgcaagagga
     4561 ccttctactg tttcaatgtc tacttgtgga aaatgcaatt ctttttctac cataccaaat
     4621 acttctaagg catcaaaaat gccatcttct tctaatgttt ttgtaatata atctgctttt
     4681 tctttgattt tatcatgaga aattcccatg gcaacgctga ttccagcata atcaaagagt
     4741 tccaagtcgt tgagaccatc tccaaaaacc atgaccttct ctggtttcaa gccaaggtgt
     4801 tccacaacct tttccacccc cgtcgctttg gagcctgaaa tcggcacaat atcagacgaa
     4861 tgttgatgcc aacgaaccat gcgaagtttg tctgagagac tgtcaggcaa gtgcaagtca
     4921 tctcccttat cttcaaaagt ccacatctga tagatatctt ctttttcatg gaaatcggga
     4981 tctacatcta agtcgggata aattggattg atagcttcac tcatcatatc ggtgcgagtc
     5041 gacaacttgg catcatgact cccaaccaag ccatactcaa ttccttcttg cttagcccaa
     5101 gagatatact cctcaacatc tgacttttca atctgatgct gataaatgac ctgacctttt
     5161 ttatcttcga tataagctcc attcaaagtt acaaaaaagt caggcttgag atcacgaatc
     5221 tctggaacaa caccaaaaat gccacgtcca gaggcgattc ctgttaaaat tcctttttca
     5281 cgcaactgtt taaaaacagt gggaattgta gttggaataa accctgtctt tgaattccgc
     5341 aatgtatcat caatatcaaa aaagacaatc ttgatcttct ttgccttgta tcttaatttc
     5401 gcgtccatct cactacctct ttcaatctaa ctctttccat tatatcataa agtaggcaaa
     5461 tcccctattt tcaaaaagtt tatcattttt attttaattt cttggatgag aaaaaagaca
     5521 tatttatgaa aaagctccat cgtgctttta atgtgttctc ttgttttcaa actcgtaaaa
     5581 agggagccac tgagcctaac tcgctctctc atttcaaagc ttgtgaaaaa agacccgttg
     5641 gggtcttaat tcgctttctt gttttcaagc tcatgaaaaa gagacccaac cgggtctttt
     5701 ctttaatctt cgtttacgaa aggcatcaaa gccattacgc gagcgcgttt gatagctgtt
     5761 gttactttac gttggttttt agctgaagtt cctgttacac gacgaggaag gattttccca
     5821 cgttctgaaa cgaaacggct aagaagctca gtatctttgt aatcaacata ttcaattttg
     5881 tttgctgcga tgtaatcaac ttttttacgg cgtttgaatc cgccacgacg ttgttgagcc
     5941 atgttttttc tcctttataa gtttagttgt ccattagaat ggtaaatcat catctgaaat
     6001 atccaatggg tttgttgctc caaatggatt ttcattacgt gaaaagtctg gtactgaatt
     6061 tgtaggtgct gaatagtttg cagttggtgc agagtaagct ccacctgtgt ggccctcacg
     6121 cacactacgg ctttccaaca tttggaaatt ctcagccacg acctctgtca cgtagacacg
     6181 ttgtccttgc tggttatcgt aactacgagt ctggatacga cctgtcaccc cgataagtga
     6241 gcctttttta gcccagttag caagattttc agcctgttgg cgccacataa cgacattgat
     6301 aaaatcagcc tcacgttcac cattttgact cttaaatgta cggtttactg caagagtaaa
     6361 agtcgcaact gctacatttg atggggtata acgcaactca gcgtcacgtg tcatacgccc
     6421 tacaagtaca acattgttaa tcatagttta ccttcttacg cgtcaatttt gacgatcatg
     6481 tgacgaagaa tgtcagcgtt gatttttgaa agacggtcaa actctttaag agctgcatcg
     6541 tcatttgctt caacgttaac gatgtggtaa agtccttcac ggaaatcttg gatttcgtat
     6601 gcaagacgac gtttttccca agtttttgat tcaacaacag ttgcaccgtt gtcagtcaaa
     6661 atagagtcaa aacgtgctac caaagcgttt ttagcttctt cttcaatgtt tggacgaatg
     6721 atataaagaa tttcgtattt agccattgat atgttcctcc ttttggtcta atgaccccaa
     6781 gactttgcaa ggggtaagtg aggttcgctc acaacaaact attatactag aaaaaatttt
     6841 tttacgcaag taaaaacact agaattcgaa aaaacgccac atgggcgttt tcctgttctt
     6901 atggtttgat acggtgcaac atacgtggga atggaatagc ttcacggata tgttttgttc
     6961 ctgctgcgaa ggttaccata cgttcgatac cgataccaaa tcctccgtgt ggaactgtac
     7021 cgtatttacg aaggtcaagg tagaattcat attctgtacg atccatgcca agttcatcca
     7081 tcttagcgac aagggcatcg taatcttcct cacgcataga cccaccgata atttctccat
     7141 agccttctgg agcaagcaag tctgcacaaa gcacgcgctc tggatttcca ggaactggtt
     7201 tcatgtagaa ggccttgatg gctgctggat agttcatgac aaatgttggc acaccaaagt
     7261 ggtttgaaat ccaagtttcg tgtggtgacc caaagtcatc accatgctca agatgctcgt
     7321 agtcagcatc ttcatcattt tcatgctctt gcaagaggtc aatggcttga tcgtaagtga
     7381 tacgtttgaa tggctctgca atgtagcgtt tcaagagttc tgtatcacgt tccaaggttt
     7441 ccaaggcttg aggcgcgcgg tcaagaacac cttgtagaag agctttcaca taagcttctt
     7501 gcaagtcaag cgactcatca tgtgtcaagt atgagtactc agcatccatc atccagaact
     7561 cagtcaagtg acggcgtgtt tttgattttt cagcacggaa aactggacca aagtcaaaga
     7621 cacgaccaag agccatagcc cctgcttcta ggtaaagctg acctgattgg ctcaagtagg
     7681 ctggcgttcc gaagtagtca gtttcaaaga gttctgtaga atcttctgcc gcatttcctg
     7741 aaagaattgg gctgtcaaac ttcataaaac cgttcttgtc aaagaactca taagttgcat
     7801 agataatagc gttacggatt tgcaacacag ctacttgctt acgagagcgt agccacaagt
     7861 gacggttatc catcaaaaag tctgttccgt gttcttttgg tgtgattggg tagtcttgag
     7921 attcaccgat cacttcgatg tctgtgatgt ccaactcata gccaaattta gaacgttcgt
     7981 cctctttgac aatacctgtc acataaacag acgtttcttg gctcaagcgt ttgataacat
     8041 caaacttctc aagtcccact tcttcaccaa atttttcgac aaagtttggt ttaaaagcca
     8101 caccttgaaa gaaggctgtt ccatcacgca attgtaagaa agcgattttt ccttttcctg
     8161 atttgttggc aacccaagcg ccaatcgtca cttcctgacc aacatagtct tttacgtcaa
     8221 taatcgttac acgttttgtc attatttttc cttttctttt ttattcttta tggcaaacca
     8281 cctctatatt gttcccatcc aggtcaatca taaaagcagc atagtaaatc ggatgctcac
     8341 tccgataacc aggagcccca ttgtctcgcc cacctgcctc taagccagcc tcataacaag
     8401 cctgaacttc ttccttattt tctgctaaaa aagcaaaatg aacaggatct tgtgttccct
     8461 gagtcagcca aaaatcacca ccaggatgag ggctgttcgg ggatagaaaa ctaattagag
     8521 aactagtctt aaaagccaat ttatagtcca aaggagcgag aaaactccta taaaatcctt
     8581 ctgaaatttg taaatccttt accttaatct caaaatgatc aatcattctc actacccata
     8641 aatgctttca agcgttcgac tgcttcttta agcgtgtcta ggtctgtcgc atagctgagg
     8701 cggacatttt ctggtgctcc aaatccagct cctgttacca aggccacttc ggcttcttct
     8761 aagatagcag ttgtaaagtc tgtcacatcc gtgtagcctt tcatctccat ggcctttttg
     8821 acatttggga agagatagaa ggccccttgc ggtttgacca cttcaaatcc tggtacctct
     8881 gcaaggaggg gatagatggt attaagacgt tcctcaaagg cctgacgcat gctttctaca
     8941 gtatcttgct cacctgatag agcctcaact gctgcatatt gggctactgc tgacggattc
     9001 gaagttgttt gacctgcaat cttggacatg gcagcgataa tatctgcttc tccaacggca
     9061 taaccaatcc gccaaccagt catggcatag gttttagaca caccattgat gaccactgtt
     9121 tgcttgcgaa tcgcttccga taggctagaa atcggtgtga actcatgacc attataaacc
     9181 aagcggccat agatatcatc tgctaggatg agaatatcat tttctacagc ccagtttcca
     9241 attgccaaga gttcctcacg ggtgtaaatc atacctgtgg gattagatgg cgaattcagc
     9301 accaaaacct tggtcttgtc agtgcgagct gcttctaact gatctacggt caccttaaag
     9361 tgattgtctt ccttagtaga aacaaagacg ggaacgcctt ctgccatctt gacctgatct
     9421 ccatagctaa cccagtatgg ggttgggatg atgacttcat cacctggatt gaccacagcc
     9481 ataaagaagg tatagagaga atatttggct cccgcagcga ctgtcacttg atttgacgct
     9541 acagaatagc cgtaaaagcg ctcaaagtag ctattgaccg ccgccttaag ctctggcaga
     9601 cctgaggtta ctgtataaaa agaagcacgc ccatctcgaa tcgatgcaat ggcggcatct
     9661 tggatatttt tgggagtagt gaaatctggc tcacccaagg ttagagacaa aatatctcta
     9721 ccctcagcct tcagtgcttt ggcacgggct ccagcagcca aagtcacact ttcttccatt
     9781 tctaaaacac ggttggatag tttcataggc cctccttgtt gaccaatgct cctgtttcaa
     9841 aatctactag ataaaaatca gatcctgact taacttccca gattggctta tcttgataac
     9901 ggccaaaggt tatcttgtca atctcgccag ctcccttttc cttagaaacc gtttctgctt
     9961 tttcttgtga aacaccctga tttagctgat aaacgtaaat cttatggtca tctttaccaa
    10021 tcaggacagc aagcgcttct tgctgtttat tacgaccaag aacgctgtaa taagattcca
    10081 agccattgta taaatcaacc tgatcgacct gctctaatcc tgcatactgc tgagctaatt
    10141 tttctccttc acttttagct gtttgatagg gtttcatgct aagagaaacc atatacagaa
    10201 aggaaccact gataaccaca aacaaaatcg tcatccctag accatactgc cacagtagat
    10261 tattttttgc tttgttttgt ctttttttca ctcgtctatt ttaccatcta ttaagcttta
    10321 ttacaagtga atataagaat actcttcgaa aatctcttca aaccacgtca gctttatctg
    10381 caacctcaaa gctgtgcttt gagcaaccaa ttctatttct cccttcaaac aaaaccgatt
    10441 ttgaaagtga aacagttctt actttttcag tcacaaatga ttagagtttc cgaaatttta
    10501 caattttcaa ataaactctg agcaaatttc ttgcaagttc ttttgttttg ttgtaatata
    10561 ttttataaca acgagagagt tctcgaaatt ttaagaaaaa ggagacacat catgtctaaa
    10621 aaagtattat ttatcgtcgg atcactacgt caaggttctt tcaaccacca aatggcgctc
    10681 gaagctgaga aagcacttgc tggtaaagcg gaagttagct accttgatta ttcagccctt
    10741 cctctcttca gccaagattt ggaagttcca acacatccag ctgtagctgc tgctcgtgaa
    10801 gcagttctcg ttgcggatgc tatctggatt ttctctccag tctacaactt ctctatccct
    10861 ggtacagtga aaaacttgct tgactggcta tctcgtgccc ttgacttgtc tgatacacgt
    10921 ggcgtttctg cccttcaaga caagtttgtc acagtatcat ctgtagccaa tgcagggcac
    10981 gatcaacttt tcgctatcta caaagacctc ttgccattta tccgtacaca aggcgttggt
    11041 gatttcactg ctgcacgtgt taatgactct gcctgggcag acggaaaatt ggttcttgaa
    11101 gaaacagtcc taaactcact tgaaaaacaa gctcaagact tggtcgaagc gattcagtaa
    11161 aaaatagaaa attttagaaa gtctaaatca attatagcca cctaataggg tggcttttta
    11221 tttctcaatc aagattttca gacaaacatc agccatccaa aaaaaccttg agcgttacca
    11281 ctcaaggttt ttagcagtct tttccatcat aggaaaatca ttctaagaat attctttcat
    11341 tcgagatcta tcacttatca tcttctttcg agaaattagt gactgctttt ggctgattaa
    11401 tcttcttttc ttcgtcctac atataggctc aatagaatac cgctacctaa aagaacagag
    11461 atataatcag attggcttcc agtttcaggt aatttgtccc cacggttttc tttacctgta
    11521 gttggtgcta ctggattagc atttactgga gttgctggtt gagccggtgt tggtactact
    11581 ggttgttctg gaaccgctgg tactggttta accggtactt ctggtgttgg aacagctgga
    11641 gttggttgta ctggcacttc tggtgtcggt acatttggtg ttggttgcgc tggcacaggt
    11701 ttaaccggta cttctggtgt cggcacattt ggtgttggag gaactggttt gtcatttgat
    11761 ggtgttactg gtttgtcttg gcatccacat tttccatctt taccgtcttt aatcaccatt
    11821 tctgtcttac ttccatctgg attagtgatt gtaacaacgt gagttccatc gttattattt
    11881 ttaacagtta cagttggtga aacgctatct ttgccgtctt taccatcttt gataatagat
    11941 ttgtattcac gaccatcaga atctacgatt gttaatgtat gagttccatc tccgttatca
    12001 acgatgttcg cttttggtga tttaccatca aagattgtag ttgtcgttcc tttaccatca
    12061 gcacctgtca ctttaatagt gtgtgaacca tcgttgttat caataacttc aagttttggt
    12121 tctctaccat cacgaactgt agttgttgta gttgttccgt ctgagttcac aactgtgata
    12181 gtgtgagttc catctccatt atccacaacc ttagcagttg gtgatttacc gtcacgaact
    12241 gtggtttcag aaacagtacc attaccgttt tccacacgaa tagtgtaagt accatcttgg
    12301 ttatctctaa ctgttgctac tggtgattta ccatctttaa tgattgtttc agtagttgtt
    12361 ccgtcaccat ttagaacaga gatcttatga gttccatttt gttcatcagt tatagtcact
    12421 tttggtgatt taccatcacg aactgtggtt tcagttgtca caccttctgg atttgtaata
    12481 acgattgtat gacttccgtc tccattatca cgaacagatg cggttggagt acgtccgtca
    12541 acaccgtcac gtccattctt aacaacaaac tcattcttag ttccgtctgg gttagtgatt
    12601 gttactgtgt ggcttccatc tggattttca gtagttgttg ctgttgcagc tttaccgtct
    12661 ttaccatctt tgatgatagc gttggtaaca ttgccgtctc catctgtaac tttaacagta
    12721 tgagttccat cgttgttatc cgttacttca actttaggag tcttaccgtc tttaccgttt
    12781 ttaacaacag tttctttagt tgaaccatct ggatttgtca ccgtaattgt gtggcttcca
    12841 tctgggtttt ctgttgtagt gatattcgca gttttaccgt ctttaccatc tttaacaact
    12901 gttgttgatt cagaactatc tgggttttga actgtgatag tgtgagttcc atctccatta
    12961 tcttttgtag taatagttgg agctgaacca tcacgtccat ctataccgtt ataaatatct
    13021 ttactattta atactttatc gacaccttcg tcgtataccc cattgtcatt aacatcatca
    13081 taaactgtaa cacggatacc gttacgcgtt ggagttgcag cagcatctgt atcgcgtcta
    13141 attcttgaag gtgaagtaat atcttctact ttgattttcg gtgcacggcc atctttacca
    13201 ttcttaccat cttttacagt gaaggttact ggatctttac ctggaacagt gaatgtaatg
    13261 tcagtactat ggccatcttt acctggagtt acagttactt ctggagtctt accatcttct
    13321 ccgcgttcac ctttgtcccc tttaggtccc ttgatgttgc cctctttatc ccagttgccg
    13381 ttattcttaa cgaagacgtc gcctgtttct gtattgacgt atttatcacc gtctttacct
    13441 tgatttactt ctggattagc tttgccgttc aacacgtctt taccgtcgcg tcctgcttgt
    13501 ccctgagctc cgtcttgacc atcacgacct tgaagacctt gttcaccttt ggcaccatct
    13561 gcaataataa cttcttttaa tttcttatca gaagcatcta attcattatt tccattttga
    13621 tcataataga agataacagt tgttgtctta tctgcttcat ttcgttgagt cgcaactaat
    13681 ggacttgcgc catcgaaacc atctcttaca acagtacttg caacattacc tctaccgtcg
    13741 ttaatagtga tagtatgagt gccgtcattt ttattatctt taacggttac ggttggagtt
    13801 acagcgtcac gacctgcctg accttgcgct ccagttagac cgcgttcgcc tcggtctcct
    13861 ttttcacctt tagtaccttg cgctccggtt agaccgcgtt caccctgatc tcccttttca
    13921 cctttagtac cttgcgctcc ggttagaccg cgttcgcctc ggtctccttt ttcgccttta
    13981 gcaccatctg cacctttgtc acctttcggt cctttgatgt ttcctgctgg ttcccaagtg
    14041 ttacctttct taacgaatac atctcctgtt tgtgtattta cgaatgtgtc tccatcttta
    14101 ccttctgttg ttgggtctgc ttttccgttt aatacttctt ttccatcacg tccagatgct
    14161 cctgcagctc cgtcttgtcc gtctttaact actgttgttt gaactaactc gtcttttcct
    14221 gctgtgtaac ttccatctcc atctttgtca atgtagaatg ttaatgttgt ttgtttcttc
    14281 gcatcatctc gttctgcttt gacttttggt gtttttccat cttcaccgtt tttaacgatt
    14341 gttgttaatg atggtctgtt gtctggttgt gtgattgtga tggtgtgtgt tccgttattg
    14401 ttatctgtaa ctgttacttc tggtgtgaat ccatcttttc cgttatcacc tttgtcacct
    14461 tttggtcctt gttttccatc tgcgataaca acttctttaa gttttgtatc tccttcatca
    14521 aattgattat tatcatttag atcataatag aaaataacag ttgttgtttt atctgcttca
    14581 tttctatgag tagaaacaag agggcttgca ccatcaaatc catctcttac tactgtagta
    14641 gttataccac ctttaccatc gttaattgta attgtatgcg ttccatcttg accatcagtt
    14701 actttaactg ttggggtttt cccatcttct ccttttggtc cttgttcacc atcacgccct
    14761 tgaagacctt gttcaccttt ttcaccttta gctccttggg ctccggttag accgcgttca
    14821 ccctgatctc ccttttcacc tttagtacct tgcgctccgg ttagaccgcg ttcaccttgg
    14881 tccccttttt cacccttggc tccatcttta atagcaattt tttgaacaag ttcgtctacg
    14941 ccctcagtat aaacaccgtt tccatcgtta tcaagataga aagtgaggtt atttgtatca
    15001 ccaactttat tttgatcaac acgtggtttt actgtatcga ttaactcttt agccacacca
    15061 atttgcataa tacgtggctt agcttcttta gtaactctat tacaattggc aatcctaaaa
    15121 gtgtttcgtt accaaatcga tgtactttat aggtaatttg acgttgtcct ttttcacctt
    15181 ctcgaactaa tctagtttga ccttctggta acttactagt tggaagatag acagtttgga
    15241 agtcaatgtc ttctgaaaca gtcttattat tagtaccacc ataagggttt gcatccaaga
    15301 ggagtggatt tgctctatga taatagttgg agttactgta tgggtcattg ctagtagcgg
    15361 caccaccacc accttggtcc acgttaaaag taactggttt caaacgaaca gacaaatcac
    15421 catttggatt aatgataggc aaactaaatc tgatagtttg ttccgtattt gttgaagtag
    15481 cagaggtcat actgtatcct gagtgagata cgtagttggc accctgacta atagattttg
    15541 attgctccct tactcttgga tttgaacttg tgatcgttgc atttgaagga tttccaaatc
    15601 catccccttt atccaagata aaattattat taacaaattc acctggacca gagtatttaa
    15661 ttgtgaaatt caatctatta cctctacgta ctgtcgttac atcataatgg atttcacgac
    15721 caccacgtga atgtcctata ccacgaaatg aatgtaagcc ctgactgatg tcagaaaatg
    15781 caatagtatt atttggattt aagattggtt tattttctgg tccagctgct ctaaaaccac
    15841 ttccagccgc tctaaagcca cttcctgctg ttctaaaacc actataccct ctatcaacat
    15901 cttccttacg gatagctgga ttttggcgag catcagaaag gctcaaactt ggtttcaaga
    15961 tttcattagt agatgctgga gctgaagttg tttctacttt agcagctgtg ctaattggca
    16021 aaatagcgct atcaacagct gaacgttcag tcgttgttac agctggttta gtctcttcct
    16081 tcgcttcaac tatattagct ttctttgcta caggcgtttc tgatacagtt cctttgttag
    16141 agttagaacg aagttctaga ccaataatag atgattgaat agatgactct gctgctgata
    16201 attcagcttc agatgcatct gctttcccaa gcaagctttg accagcttgg atacttgttt
    16261 gcaagttagc agcacttgct tctgtatata acttgctttc taacttttta gcacgcgaaa
    16321 gagccgttaa caattttgac ttgtcaatag catcttgatg cgttacttcc tcttttttgt
    16381 cttttgcttc ttcaactttt tctgctggtt tagtaacttc tccagtttta actggagtcg
    16441 tttctactgg attagctaca gttggagctg cataggtttt ctctttatta gacgcagaaa
    16501 cctctgctgg tttagtagct gcttctcccc taccaacaga aatactattg gtagtttctt
    16561 gtttgttttc ttccgcttgt actgtttgga cactagctgc tgcacctaat accaaagcgg
    16621 taccgagcaa gacactaact gcaccaaagt aatacttacg aatagaataa cgttgttgca
    16681 tgctatacca gtcaaatgat tttttatgtg aatgtttcat tttaaactcc tagatagtat
    16741 t
//
LOCUS       DAVLFB010000039        16542 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLFB010000039 DAVLFB010000000
VERSION     DAVLFB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.039"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            181..1182
                     /locus_tag="VXD58_001127"
                     /pseudo
     CDS             181..1182
                     /locus_tag="VXD58_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1299..1646)
                     /locus_tag="VXD58_001128"
     CDS             complement(1299..1646)
                     /locus_tag="VXD58_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV0675838.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1757..3532)
                     /gene="spxB"
                     /locus_tag="VXD58_001129"
     CDS             complement(1757..3532)
                     /gene="spxB"
                     /locus_tag="VXD58_001129"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV0675839.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3739..5982)
                     /locus_tag="VXD58_001130"
     CDS             complement(3739..5982)
                     /locus_tag="VXD58_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV0675840.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5992..6363)
                     /locus_tag="VXD58_001131"
     CDS             complement(5992..6363)
                     /locus_tag="VXD58_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV0675841.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(6374..6769)
                     /locus_tag="VXD58_001132"
     CDS             complement(6374..6769)
                     /locus_tag="VXD58_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV0675842.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6953..7051
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7135..7926
                     /gene="thiD"
                     /locus_tag="VXD58_001133"
     CDS             7135..7926
                     /gene="thiD"
                     /locus_tag="VXD58_001133"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV0675843.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(8136..8768)
                     /locus_tag="VXD58_001134"
     CDS             complement(8136..8768)
                     /locus_tag="VXD58_001134"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0675844.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8761..9564)
                     /locus_tag="VXD58_001135"
     CDS             complement(8761..9564)
                     /locus_tag="VXD58_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0675845.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9564..10088)
                     /gene="thiW"
                     /locus_tag="VXD58_001136"
     CDS             complement(9564..10088)
                     /gene="thiW"
                     /locus_tag="VXD58_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV0675846.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(10093..10785)
                     /gene="tenA"
                     /locus_tag="VXD58_001137"
     CDS             complement(10093..10785)
                     /gene="tenA"
                     /locus_tag="VXD58_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV0675847.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10796..11446)
                     /locus_tag="VXD58_001138"
     CDS             complement(10796..11446)
                     /locus_tag="VXD58_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV0675848.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(11448..12833)
                     /locus_tag="VXD58_001139"
     CDS             complement(11448..12833)
                     /locus_tag="VXD58_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675849.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12834..13394)
                     /locus_tag="VXD58_001140"
     CDS             complement(12834..13394)
                     /locus_tag="VXD58_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0675850.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13656..13744)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13953..14582)
                     /locus_tag="VXD58_001141"
     CDS             complement(13953..14582)
                     /locus_tag="VXD58_001141"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV0675851.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14584..15366)
                     /gene="thiM"
                     /locus_tag="VXD58_001142"
     CDS             complement(14584..15366)
                     /gene="thiM"
                     /locus_tag="VXD58_001142"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV0675852.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPE
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(15603..16271)
                     /locus_tag="VXD58_001143"
     CDS             complement(15603..16271)
                     /locus_tag="VXD58_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV0675853.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
BASE COUNT         5018 a         3602 c         3046 g         4876 t
ORIGIN      
        1 cgccttcaaa tcgttctata gtaaaacgaa acaagaacag ggcaaatcga tcaggacagt
       61 caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatttatt
      121 atatttcgtc tgatgggcaa atcttataaa gagattatag aacttttata gtagtttgaa
      181 ataagatgtg aacaactcta tcaggaaagt caaactaatt tatagaaata ttttagcagc
      241 taaggtgtac tgttatagat tcaatacact atagactgta atcaaacaac gatttggcga
      301 aatgtaaaaa aatatgagga gttcgaactc gactctctcc ttcaagaaac acgtggtggc
      361 cgtaaccatg catatatgac gattgaggaa aagaaagtct ttcttgcccg ccatttgaag
      421 gctacagagg caggagaatt tgttacaatt gatgccttat ttcaggctta taaaaaggag
      481 ttaggtcgtt cctacacacg tgatgccttc tatcaactgt tgaagcacca tggttggcga
      541 aatattatgc cacgtccaga acatcctagg aaagcagacg ctcaaaccat tgtcgcgtct
      601 aaaaataaaa tctcaattca agaagaaaag aaagcgcttt aaaagcagta gacgttttcg
      661 taaggttcgc ttgatgtacc aagatgaggc tggtttcggt agaatcagta aactgggatc
      721 ttgttgggct ccaataggag taggtccaca tgtccatagt cactatatac gagaatttca
      781 ctattgttat ggagctgttg atgcccatac aggcgaatca tttttcttaa tagctggtgg
      841 atgtaatact gagtggatga acgccttttt agaagagctt tcacaagctt atccagatga
      901 ttatctttta ctcgttatgg acaatgctat atggcataaa tcaagtacct taaagattcc
      961 gactaatatt ggttttacct ttattcctcc atacacacca gagatgaacc cattgaacaa
     1021 gtgtggaaag agattcgtaa acgtggattt aagaataagg cctttcgaac tttggaagat
     1081 gtcatgaatc aacttcaaga tgtcatacaa ggactggaga aggaggtgat aaagtccatc
     1141 gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata tgcaatttct
     1201 ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca atttctattt
     1261 taaaaatagc taaggttaac gtcaaatgac tacgcgacct atttcatacg ataaaaatca
     1321 agcactagac cagcaggtcc ttgaactaat aaggactctg ttccccaatc ggttacagtt
     1381 ggtccgtgta aaacctttat accaagctcg ttcaaccgtt tgtagttctg gtctacatcc
     1441 tcaacctcga tatgaataat gattcctgac tgaaagtttt ccaaaggaac caaatgattt
     1501 tgtgacaaca taaggcagtg actaccaatc gtaaactgag caaaaccatc atcagcataa
     1561 tctgcctttt tatccaaaat atgctccaag tcagcacaga cttggggaac atttgaaacg
     1621 ataatatcta attgatttaa attcatttac tctcctccat aaaaagaccg gattgctccg
     1681 atcttttaaa gttctgctct atgaaaatca aagaataaag tctacaagtt tcatatttga
     1741 ttttcggcga gaggaattat ttaattgcgc gtgattgcaa tccttcttct tccaagaaga
     1801 gacggaatgg tacgagttct tctgcttcgt atttttcctt gaaggctttg atagcttctt
     1861 ctgagtgaag ttttggatcc aattcaagta cttctactgg aagtggacgg tgttgagtga
     1921 tgcgagcatc gatgacaaca gttttacctt ctttgttcaa tttaacagct tctgcaacaa
     1981 ctgcatcgat gtcttcgata cggtcaactg tgaatccaac agctccttga gcttccgcaa
     2041 ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt gtatcttcgt
     2101 atttgttctt gatgaagccg tactcagcat ttgagaagac aaggttgata actggaaggt
     2161 cgtattgaac gtttgtgata acgtctgggt agcacatgtt gaatgctccg tcacccatga
     2221 tgttccatac ttggcgatct ggattgtctt tcttagcagc gataccacca ggaagggcaa
     2281 tacccattgt cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc atgtgaaggt
     2341 gacgagtaga tgtttgagta gtgttaccta cgtcgattga gtagatagcg tcttgatcag
     2401 catgtttgtt gattgcattg taaacttgat acaattgcaa ttcaccctca gttttacctt
     2461 cgagtttgtt catgtaatca cgccagtttt ggttgttctt aacgtttgca cgccaccatg
     2521 gagttgattc aactgggttt actttgtcaa ggatagcttt agctgcttga ccagcatcac
     2581 caaggattga agcgtcaagg gcatgacgtt taccaagttt gtaagggtcg atatcaactt
     2641 ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc agcaaatggg aagtttgaac
     2701 caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc caaccaacac
     2761 gataagcaga acctgtcaaa ccttcatagt tccattcgaa agcttcaaag tttttaccag
     2821 ttgtgatgat tggtgctttg attttacgtg acaattcagt aatcacttca ccagctttaa
     2881 caccaccaaa tccagcatag ataactgggc gttcagcatt gttcaagatt tcaacagctt
     2941 tgtcgatttc aacttcgttc aaagcaggag cgatgaatga gcgttcgtat gaacctgaac
     3001 cgtagtatga gttttcgtcg atttcttgga aaccgaagtt tactggaatt tcaacaacag
     3061 ctggaccttt tttagaaact gcagcacggc aggcttcgtc aattactttt ggcaattgct
     3121 cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt gtacattggg ttttggttaa
     3181 gctcttggaa agcatccatg ttcaattcgt taactggacg tgatccaagg attgctagga
     3241 atggagtgtt atccatagct gcatcgtaaa caccgttaat caagtgagtc gcacctggac
     3301 cacctgaacc aactgcaacc ccgattgagc cgccgaattt agcttgcata accgctgcaa
     3361 gagcacctgt ctcttcgtgg cgaacttgta agaagcggat atctttgtct tcagccaaag
     3421 cgtccatcaa tgagctgagt gttcctgatg ggataccgta gattgtatct acgccccatg
     3481 ttttcaatac gttaagcatt gctgcagatg cagtaatttt cccttgagtc ataatgataa
     3541 ctctccttca atttttttag acttggagaa tacgattaca tagaattgga aacgttctcc
     3601 aattttttac tattccactg tatcatattt atgctgactt ttctaaaaat ctgctcaaaa
     3661 ctctctattc tctattctaa tacagttttg aaagtgtcgt catttctgtt ttataacaaa
     3721 aaaatctagt cattactttt agtctatttt actaaaattt aacagaaggg aactggtcag
     3781 aacagataca gaactaaagg ccatggctag acctgccaat tctgggttga gagccagtcc
     3841 aacacctgaa aagactcctg ctgcaatcgg aattccgaca acattgtaga taaaagccca
     3901 gaaaagattg agcagaatac gatgaaaggt tttcttactc atgtcgaagg cacgcaccac
     3961 tcctaaaaga ttattggttg tcaacaccaa atctgctgac tcgatggcga tatctgttcc
     4021 agctcccata gcaatcccca catctgctac actaagggca ggagcgtcat tgatcccatc
     4081 accaacaaag gctactttac ctgactgttg cagtttatgg atttcatggg ctttttcttc
     4141 tggcaagacg cctgcaatga cctcttcaat tccgatttga tctgcaatag cacgcgccac
     4201 accagcattg tctcctgtca gcatgactgt cccgagacca cgttttttta gctgactgat
     4261 ggctagctta gcatttttct taggaatatc ttgcaaagca agcaagcctt tgatttcatt
     4321 gtcaacagct aagaacacaa ctgtcttagc ttctttttct agttcttcta gtttatcttg
     4381 ataagtatta gaaatatcca tgccatccag cattttagca tttccaagta aaacttgttt
     4441 tccattgatt cgccctgaaa cacctttccc gtgcaaggcc tgaaaatttt caacagtttg
     4501 aaactcaagt ccagcttcac tcgctcgctt aacgatagcc tcagccagtg ggtgttgaga
     4561 agcatcttcc aaggaggctg ccaatccaaa cacttctact tcgtcgccga tgacatctgt
     4621 taccacaggt ttcccttccg tcaaagtccc ggtcttatca aagacaaggg tttgaacttt
     4681 ctggatttcc tgtaagacag ttccattttt gaggagaacc cccatcttgg cactacgtcc
     4741 tgtccccacc ataagggctg tcggtgttgc aagtcccaag gcacaaggac aggcgataat
     4801 caaaaccgcc actccgtaga gaagagagga cacaaagcta gctccaagca caaccacact
     4861 atccctgagc aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga caactactgg
     4921 gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac ttgtctgagc
     4981 tttcttcaca aaatccacaa tctgagccaa aacagtctct gagccaactt tttctgctct
     5041 aaagacaagc gttccactat gattgatggt tgagccaatg acagtatctc caactgtctt
     5101 gtccacaggc agactctcac ctgtcaccat ggattcgtca atactagaga caccttctac
     5161 tacgacacca tcaacagcaa tcttttcacc gggacgcact cgaatcaggt cgcctacctt
     5221 gacttgttcc aaaggaactt ggacataact atcatcactc aagacttctg cggttttagc
     5281 ttgcaagtcc agtaatttct ccacagcttg ggacgtattt tttctcattt tttcctcaaa
     5341 aactgctccc aaaagaacga aaaagaggat aaatccagca ctttcgaagt aaacagggag
     5401 accagcaaag agagcaacta ggctatagaa ataagccact agagttccca gcgcaaccaa
     5461 ggtatccatg ttggcattgt gctttttaaa actggcccaa gcactctgga tatatggctt
     5521 acctgcaact aacataatag gcgttgttgc tagaaaggtt ccccaatgca tgacttgatg
     5581 actaatgcta cctgccaaca tcccaatcat gagaatcaca agaggcacag tacagatact
     5641 agtaatccaa aaacgttgca ggagagatag agattttcga gtcttctcaa cgactgtata
     5701 gcttcccttt tgcatcttca tgccacaaga aaattcatgt cgccctaatt cttgaggtgt
     5761 aaaacgaatg actttctcct catctacgcc gattggttcc aagatacctt cttcttcaaa
     5821 cagaatttcc ttataacagt ttgaaggagt agcacgatga aaggtaatct cagctggaat
     5881 tcccttttga agctggatat gggctggatg atagcctttt tcagcttgga tacggatttt
     5941 ttgaatgcca ttttctaagc ttgctttcac aatttctgtc atagtctcca cctactctac
     6001 aatcatcttg ccgtgcatca tgttcatacc acaagcaaag ctaaactctc cagcctgttc
     6061 aggcgtgatt tccactacat actcttcccc cattggcagg ttcgcatgta caccaaaatc
     6121 tggaaaaaca atttgatcca gacatggtga aggatccttg cggtcaaaga caatgcgggc
     6181 tggcactgat ttcttgagga caatcaactc aggagtatag cctcccatga cttccactcg
     6241 aatctcttgg tatccgtttt tttgctgggc tttttgtcca gatttttcag gctttttgaa
     6301 aaaccaaaac aagataaacg cgataagggc aatacaaata atggttacaa tactatttaa
     6361 catgacgtct cctttacata caattacatc ttacttctgt tacagcactt gatttcttct
     6421 ctgaaatcac agcttccaag tcttccaagt cagtctgagt aaattcacat tctacaatca
     6481 agtcagccaa caaattccta atcctacggg aacaaacctt gtctttgata tcttggacaa
     6541 gtaaatcccg actttggtct agagttaaaa gcgctgaata aacaaaggac ttgccttctt
     6601 ttttccgagt caaacactct ttctcaacta gacgagccaa aagtgtctga accgtggact
     6661 tggaccagtc aaaccgctct gccaaaaccc taatcaaatc tgtactggtc tgctccccct
     6721 gcatccaaat aatcttcatg acctgccatt ctgcatctga aatctgcatt accatacctc
     6781 caaaatctac atttgtcaat tacactcaac agtatactcc caaaatctac atttgtcaat
     6841 tatagaaata atattttctt cgaaaaatag aattttaatc atttgaaaaa cgatttgcag
     6901 tcaaatatta ctatataaac aataaaaata tgctatacta aagaaaaaag aaaacaacca
     6961 ctaggggtgc gtaaagctga gattaacgac tgttagatcc ctctgactca atctaggtaa
     7021 tgctagctga tggaagtgga aatgataatg gggactagca gtcttctatt gcctttctaa
     7081 aacagactag cttgttctta agaatacaaa cttcagttgg ttgggaggtt ttagatgact
     7141 tatttacccg ttgctttgac cattgcaggg actgacccta gtggtggtgc tggcattatg
     7201 gcagatttaa agtcattcca agcgagagat gtctatggaa tggctgttgt aaccagtctt
     7261 gtcgctcaaa ataccagagg tgttcagcta atcgagcacg tttctcctca aatgttgaaa
     7321 gcccaattgg agagtgtctt ttctgatatt ccacctcagg ctgtaaaaac tggaatgttg
     7381 gctactactg aaatcatgga aatcatccaa ccctatctta aaaaactgga ttgtccctat
     7441 gtccttgatc ctgttatggt tgctacaagt ggagatgcct tgattgactc aagtgctaga
     7501 gactatctca aaacaaactt actacctcta gcaactatta ttacgccaaa tcttcctgaa
     7561 gcagaagaga ttgttggttt ttcaatccat gaccccgaag acatgcagcg tgctggtcgc
     7621 ctgattttaa aagaatttgg tcctcagtct gtggttatca aaggcggaca tctcaaaggt
     7681 ggtgctaaag atttcctctt taccaagaat gaacaatttg tctgggaaag cccacgaatt
     7741 caaacctgtc acacccatgg tactggatgt acctttgctg cagtgattac tgctgaacta
     7801 gccaagggca agagtcttta ccaggcagtt gataaggcca aggcctttat cacaaaagct
     7861 attcaagatg cccctcaact cggtcatggt tctggtccag tcaaccatac aacttttaaa
     7921 gattaagaaa aaaaactctc tagttcccac tttaagggaa ttagagagtt tttatactct
     7981 tcgaaaatct cttcaaacta cgtcagcttc catctgcaac ttcaaaacac tgttttgagc
     8041 tgacttcgtc agtcttatct aaaacctcaa ggcagtactt tgagcaacct gcgactagct
     8101 ttctagttta ctctttgatt ttcattgagt attaattagg aaagaatgtc atgcaacttt
     8161 gttaaaaagg cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc acgtacaaca
     8221 gctacaccag ctatgccagt gcccataagc tgatcaatat tctccgaagt caagccacca
     8281 atagcaacta ttggaatggc aaccgtttgg caaattgttt tcaaggttga aatcagggtg
     8341 atgggtgcat tttccttggt ggttgtcgga aaaatggctc ctgtccccaa gtaatctgca
     8401 cctgatttct ccgcttccag agctctttta accgttttag cggtgacacc gaggattttt
     8461 tcaggaccca agacttgtcg ggcaaccgaa actgggagtt cgtcatctcc aatatgcaga
     8521 ccagcagcat caaccgcaag acaaacatcc aaccgatcat cgattatcaa gggtacctga
     8581 taagcatctg ttatttcctt gacttgtttt gccagttgat aatattgatt ggttgtgaga
     8641 tttttttctc gcaattggac tatggtaacc cctgaacggc aggccgtctc aacttttgca
     8701 agaaagcttt ccacggaatc ttgatagcga ttggttacca gatatagtct aagtgcttct
     8761 ctattcataa acctctcctt tgatggtatc tagccaattt tcatctcttc ttaggagcga
     8821 aagctgattg agtacttggt aacgaaattc ttccaatccc attccttgaa caactatttt
     8881 ctcagcagcg atattgagat aagagactgc taagcaagaa gcttcaaaac cagtctttcc
     8941 ttggctgaga aaaacggctg ttaaggctcc aaccaaatct cctgtccctg ttatccagtc
     9001 taattctgca cagccatttc ccagtacagc gacctgattt ttcgaaacga cgaggtcctt
     9061 gggacctgtg actaagaaag acataccatg ataggtctga caccagtctt tcaagacttg
     9121 aagcaaatcc tccgtttctt gatctttagc actcgcatcg accccaacgc cgtggtgctt
     9181 taatccaaca agacttcgaa tttctgacat gtttccttta aggaccgtag gtctatagtc
     9241 taaaaggtct ctgactaagc tcttacgaat ggatgaagct gttacgccaa ccgcatctac
     9301 taccattggg agacaaactt gagctgcata agaagctgct atacggattg ctttctcctt
     9361 ctcagctgac aaatgcccca aattgatgaa gagagcctga ctttgcttag taaaatcaag
     9421 aacctcacgg ggatcatctg ccatgacagg tttgcatccc agagccaaaa tcccatttgc
     9481 cagcatctca caagaaatct cattggtcat acagtgaatg agggaactag agcctatagg
     9541 aaagggattt gtaaattcct gcatcagtct atcctttcac taaagaaata tccctgcact
     9601 tttttaaaga attcctgctt gattaaaaat cgaaaggcaa taaaggaaat cgctgtacca
     9661 atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt agcagctgat
     9721 cctgtaaaga gtaccataac aggataggaa acaatagaac caataatacc tgttcccaca
     9781 atttctccca aggcagaaaa gtaaaatttt cgaccgtact tataaaagag acctgctaga
     9841 agggctccaa aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt cgtcatacgg
     9901 ataaaggctg tcactgtagc catagccaag gcataaacag gtcccatcat gattcccgct
     9961 agaatattga ctacactgga catcggtgcc attccctcaa tccgaaagat aggtgtaagg
    10021 actacatcaa gggcaatcat catagataaa atggtcaatt tgtgaacttg tagttggtgc
    10081 tttctcatgt ttctattctt ctcctttttc taaagactgt aaatcgctct tccatgtctg
    10141 gtgttggtaa gccatctccc aaaacttggc ttccatatga acactgatgt ggaaggcatc
    10201 tagcattttt tgcttatctg tctcatcact ttctcgatag agctgattga ccagtgctcc
    10261 ctcctctctg atctgttgct ctaactcatc cgtaatataa gtttcaatcc attgttgata
    10321 gagaggattt ggtgatggtt taagattaag tgatttgcct atatcatggt ataaccaagg
    10381 acaaggaagc aagcttgcaa aagcgatggc taagttcggt tctgcaaatt gcctataaat
    10441 atgagaaatg taatgataac aggttggagc gattggatgt tgctccattt cctggtcgct
    10501 gatttccaat tccttgaaaa attgttggcg aataaataac tcaccctcca ctaaaccctg
    10561 agcattttgt ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag ccaaaagatg
    10621 atagatttct gagaaagcct tcagatagta ggcatcctga atcaggtaat agcggaaaat
    10681 ggcaggttct aaattcccct cttgtaattg taaaataaag ggatgatgaa aggaagcctg
    10741 ccaagctttc ttggataatt ccatcgcaat atctgtaaat tccataataa ctcctttata
    10801 aaaatagact ggtttgaagc aataaaaaga aaagcaggta gattaatttt gtttttttag
    10861 gaatataaaa agtccgatag ctattcttca actgtgcatg ttcgtcatat ccgtgagcag
    10921 atagagctct caggtaaaga tggcgccacc taaagactgt catcagaacc ttactgtaaa
    10981 tcaagggcga ccaaaaatgt agttcttgac cacgtaatag gcaagcttct ttgagggact
    11041 tgatttcttg ctgaatgaga ggaaaagaat tgaataccac aatcaaggca taggaccaag
    11101 agcgtgatag ccccttttga gccaagtaca agagaagctc ttttagtgaa atagaggaaa
    11161 caaagacaag gccgatacaa actgtcacaa aggccctcgt tccaagcatg actgcctgtg
    11221 aagcatctcc gtgtaactga actgcccagt agttggcaaa agatggtaaa atggcaagta
    11281 tgatcatcca agctaacatt ttaaatcgac ggtaatagag cataaagaga atacaaaatg
    11341 cgactaccga aagattcaga gcaatcgaag gaatgaaaga tgtttccaag gataaaatca
    11401 gcaagaagag actgataatc ggtgtctggg ttgctacttt gaccatacta tctcacctcc
    11461 ccttgggtat tgctactctg agatgtaagt ggtttggtaa tggtcacttc tttcacatgc
    11521 cgaagaccct gactagtcat ctcaatccaa taatcaacca cagaaatcaa agggtctaaa
    11581 cgatgactaa tgagcagaaa acttcttcct tgattcctct cctccacaat ccacttgcaa
    11641 aaataatggc aggctctatc atccaaacct gcaaaaggtt catctagcaa gatcacggaa
    11701 gccttactgg tcaagatggt caggagctga agaatttttt gctgaccacc acttaattga
    11761 tagggactct tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg aaaaatccgc
    11821 tgatttcttt cagaatcagg tccatctaat tgaagctcct ctcgcagact gactcggata
    11881 aactgcttct cagcttcctg aacaacacca gtcagatcac gatacaaact ctttttcttt
    11941 ttcaggaccg aacccttcca tgcaatgcgc cccttatact tttgaaattg aagaatagac
    12001 cgaaagaggg ttgatttccc gacaccattg tcacccagga tacaggaaat cccttgatag
    12061 aatgtgaaat cagcaattga aaagaggggg cgatttccaa actcacaagt cgttctatcc
    12121 atatggaata gttccgggct agaagcaact tcctttgaag caacctgtgt catctcatag
    12181 gaagggattt gaaacacttc ccttagtttt ccgtctctta gctcaaccat atggtcgata
    12241 taggctttat agtcagataa atcatggtcg cacaaaataa ctgtctttcc atcaagagcc
    12301 aactctttta gaatctccaa tatctcgatt ctgctcttgc ggtcaatgga agcaaaaggt
    12361 tcatccaaga gatagaccct aggattcatg gcaaagagga cagccagcgc tgctttttgc
    12421 ttttccccac ctgataggtg atgaatggga cggtgtaaga ttgcctcgca acgacattgc
    12481 tggacaacct ctgctatttt agaatcaatt tcctgaaggt gatagccgat attctccaag
    12541 gtgaaaatca gctcctcaaa caagctctcc atggtaaatt gatgattggg attttgaaag
    12601 agaatgccaa ccttctggac acgtgcgatg atagaaagtt gactgacctc gctcccatct
    12661 atcaggactt gaccactata gggaagagaa ctgacttggg caatcatttg aaagaggctg
    12721 gattttccag acccactact cccaactaac aaggtaaagg cttgcgcatg aaaagtaaaa
    12781 tcaaacggct cagagaagat tggggactga atcgctcgta gttccagacc catctatgct
    12841 tttcctccag ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa gatggtacag
    12901 aagaaataaa cagaaataaa acgtaccaca agtaaggaaa ggacaaacgg aagggaaaag
    12961 gcgtagtaac ctaacttaat gtattcatag acaaagctaa caagcgtaat cccaatacta
    13021 ttagcagtta gagagagcca actttcatag cgattcttag ttacgataaa accaaattca
    13081 cttcccaaac cttgaaccaa gccagacaaa agagctccta gaccaaattg gctaccataa
    13141 aggacttcag caagcgcagc tagcacttct ccaatcgttg cacttccgac tctcggaaca
    13201 aagatggcag caatgggcgc agccatacac cagagaccga agaggatttc attggcaaag
    13261 gcctgcaaac caagaggtgt taagagtaga ctgagaatat tatacacata tcctgaacca
    13321 acgaaaaccc caccaaaaaa gatagacaag aaagcaagca agataacatc ttttaactgc
    13381 cattttttca acataaaaaa ctcctttttt taaagaaaag tggggcactc aagaagaccg
    13441 acctaaatac tttgtatagc agactgaatt tagaacagta cacaagaatg ctaaaatatt
    13501 tctagaaatt aatttgaatt ttctaattga tttgttcgca tcttatttca atctactata
    13561 tcatcttcat ccagtttcgt aaaagaaaaa actctaatta cagatacgaa ttagagttca
    13621 gcttacaaga ttagacagtt cttttcgaca tacgaaaaaa acatttcaca tttcccttcg
    13681 ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cctaaagcac cccaaatgtc
    13741 tttattattt aattatgtga ttattataac acacatttta tactagttca agaaattgaa
    13801 ctggaaatac agccttgcac tcacaaagac agcagatctt tcttttgcaa aaaacaaatg
    13861 acctgtttga tgaattagcc attcaagctg aatctggaca tagcttttta aaaaaggaaa
    13921 atcctactta cttagaatcc aaggatagat atctattgtt cactcatttc ccgaacagtt
    13981 ttttctatat tttttgcata cgatattgcc gaaatgattg aaacgccatc catattggtc
    14041 tttataatgt ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa aggcatttgt
    14101 ggcaatagtt ttctcatcaa ttcaagacct tcataaccta tagcaccacc agcatcatct
    14161 tttgactggg tatcaaatac aggcccaaca cctacataat ctacatattc aacttttgat
    14221 tgttgaaatt cttcttcgtt tcttatagaa agaccaatta ttttatctgg catcaatttt
    14281 ctaatttcat caacaccaat atcatcttga cctacatgta cgccatcggc gtcaatttcc
    14341 attgctaaat ctatatcgtc attaacgata aatggaacat tgtatttttt acaaagttct
    14401 ttaatttgga tagctagctc aagtttttct aagccttcta aagcaccctc acctttttct
    14461 cgaaattgaa ataaggttat accacctttt aaggcttcct caacgactgt atatagattt
    14521 tttccttggc aagtagtcgt tccacaaata aaatatagtt ttagtaattc tttatgaaac
    14581 atcttacttc actcttttga attcctttac atcttcatct gtaatctcgt ataaggcatt
    14641 tataaattca actttaaatg tcccaggaag atgtccattt ggacgttttt ctgccatttc
    14701 tccagcgata ttgtaaacta acattgctgt ttctaatgat tttaattctt gacctttttc
    14761 cagtccgata aaacttgcta ctacagctcc taataaacat ccagttccaa taacttttgg
    14821 catcatggca ctaccattat gaatcgttac cacttctcca tttaccgcaa tagcatccac
    14881 ttcacctgtt actactattg gaatattgaa cttctcattt gctgctagag caatctcgtc
    14941 aatattatct actcccgcac tatctactcc tttagatgcc acatctattc ctactaaaga
    15001 ggcaatctct ccagcattcc ccctaatcgc agctagtttg taattgttga ttaaatcatc
    15061 tgctactttt tttctatatt ctcctgctcc acaggctaca ggatctaaaa ctgctgggac
    15121 attatatttc tctgcaattt tcagagcagc ttggtataat ttccaatttt catctgtcaa
    15181 tgttcctatg tttattaata aaccaccagc atactttaat aagtcctcta aatcttctgg
    15241 aaactcactc atggctggtg aagcgcccag tgctactaat ccatttgctg tgaaattttt
    15301 tactacatca ttggtgatgc aaatgaccaa aggtgctttt tcttttaata attttaaact
    15361 tgtcatattg aaatccttcc ttttcacttt atacgatcta ctaatttcga tttatcttta
    15421 gttgagaatt tttttcattt acattgaatg atttatactc aatgaaaatc aaagagcaaa
    15481 ctaggaggct aaccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg
    15541 tggtttgaag agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt
    15601 ctctagacat cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag
    15661 cgttgctgaa gttctgtcaa atcttctcga tttttaccga cacgattgag ttcgtcaacc
    15721 agaaattgaa cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga
    15781 atatagactt caggtaaagc caaatcttta gaaccccagt ctaaatagag accttctgca
    15841 atgaccagca taatcaaaag atgggcatag tctgatgaag ccactgccga atacattaga
    15901 tcctgaaagg cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact
    15961 tttaactctt taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt
    16021 tgcttggcaa aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc
    16081 atggataaga aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta
    16141 ttctcaattg tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa
    16201 tccttctgac tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct
    16261 tgtgtttcca tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa
    16321 atatcaagca aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag
    16381 agagctaact aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag
    16441 aattagagtt gacttacaag attagatcgt tcatttcgtc atacgaaaaa acgtttcaca
    16501 tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt at
//
LOCUS       DAVLFB010000040        16081 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLFB010000040 DAVLFB010000000
VERSION     DAVLFB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.040"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            61..528
                     /locus_tag="VXD58_001144"
     CDS             61..528
                     /locus_tag="VXD58_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0675854.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(525..1040)
                     /locus_tag="VXD58_001145"
     CDS             complement(525..1040)
                     /locus_tag="VXD58_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV0675855.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1052..1579)
                     /locus_tag="VXD58_001146"
     CDS             complement(1052..1579)
                     /locus_tag="VXD58_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0675856.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(1857..2420)
                     /locus_tag="VXD58_001147"
     CDS             complement(1857..2420)
                     /locus_tag="VXD58_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0675857.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(2404..2955)
                     /gene="coaC"
                     /locus_tag="VXD58_001148"
     CDS             complement(2404..2955)
                     /gene="coaC"
                     /locus_tag="VXD58_001148"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV0675858.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(2967..3656)
                     /gene="coaB"
                     /locus_tag="VXD58_001149"
     CDS             complement(2967..3656)
                     /gene="coaB"
                     /locus_tag="VXD58_001149"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV0675859.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            3886..5556
                     /locus_tag="VXD58_001150"
     CDS             3886..5556
                     /locus_tag="VXD58_001150"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV0675860.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            5884..7059
                     /gene="mutY"
                     /locus_tag="VXD58_001151"
     CDS             5884..7059
                     /gene="mutY"
                     /locus_tag="VXD58_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV0675861.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            7114..7818
                     /locus_tag="VXD58_001152"
     CDS             7114..7818
                     /locus_tag="VXD58_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0675862.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            7811..9160
                     /gene="vicK"
                     /locus_tag="VXD58_001153"
     CDS             7811..9160
                     /gene="vicK"
                     /locus_tag="VXD58_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV0675863.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            9162..9971
                     /locus_tag="VXD58_001154"
     CDS             9162..9971
                     /locus_tag="VXD58_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV0675864.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            10062..10304
                     /locus_tag="VXD58_001155"
     CDS             10062..10304
                     /locus_tag="VXD58_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV0675865.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            10306..10560
                     /locus_tag="VXD58_001156"
     CDS             10306..10560
                     /locus_tag="VXD58_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV0675866.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            10607..11302
                     /locus_tag="VXD58_001157"
     CDS             10607..11302
                     /locus_tag="VXD58_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV0675867.1"
                     /translation="MKKKWLFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(11651..12637)
                     /locus_tag="VXD58_001158"
     CDS             complement(11651..12637)
                     /locus_tag="VXD58_001158"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV0675868.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            12831..15299
                     /gene="gyrA"
                     /locus_tag="VXD58_001159"
     CDS             12831..15299
                     /gene="gyrA"
                     /locus_tag="VXD58_001159"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV0675869.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            15299..16042
                     /gene="srtA"
                     /locus_tag="VXD58_001160"
     CDS             15299..16042
                     /gene="srtA"
                     /locus_tag="VXD58_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV0675870.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         4930 a         2812 c         3568 g         4771 t
ORIGIN      
        1 aaagataata tctctggtag taagtctgaa aatttaataa attagaaagc gagtaaattt
       61 atgtcccgtt cccaattaac aattttaaca aatatctgtc tgattgaaga cctcgaaact
      121 cagcgcgtgg tgatgcagta tcgcgcccct gaaaacaatc gctggtctgg ttatgccttt
      181 cctggaggtc atgtagaaaa tgatgaggct tttgcggagt ctgtcattcg tgaaatctac
      241 gaagaaacag ggttgactat ccaaaatcct caacttgtcg gcattaaaaa ttggccacta
      301 gatacaggtg ggcgctatat tgtcatttgt tataaggcga ctgagttctc tggtagcctt
      361 cggtcttcag atgaaggaga agtttcttgg gtgcaaaaag accagattcc aaacttggat
      421 ctggcctatg atatgctacc attgatggaa atgatggaag ctcccgacaa gtcagagttt
      481 ttctaccctc gccgtacaga agacgattgg gaaaagaaaa tcttctagtc ttttactaaa
      541 taacctagct gatccaaggc ctcctcgata tagtggaggt cttgttgtgt ttcggcttcg
      601 actaggtggt aatggatgcc atctgtcaat tcagatagag gtctaaagtc agaatgctcg
      661 acttgttcta gaaaatgttg cacatctctg cgacaagaca gtttaagcaa ggtttcaatt
      721 tctccataaa cgggatggtc aatcaaggta ttctggacat gcccaccatt atcgacaata
      781 gcaaggagtt cctgaccgat ttcttcaact tcatgtttca ctttgaaaag tttgtgaaca
      841 taaggattgg tatcaatttg cttatagatg tagccacgat tggtggatag gataggagcg
      901 ccatcggctc tcaaaattgc aatgtcctgc acaatgacct gacgtgtgac atgaaaatgt
      961 tccgccaaac tttggccatt aagggcttta ggagcttctt tcaagagttg gagcagggct
     1021 tgtttgcgat cttttgtcat agcttttcct tttaacggcg ttttcgaagc actttataga
     1081 cagctagtgc taatgtatag tctaccatac tatggataat tgtgccaaat ccaactagta
     1141 caaatagaac atagaacata ttttctacat tggtacccga agttgcgtaa aaaaggacac
     1201 aggctaatac ttcagcaagg gcatgaacaa cagccaaaac aaaattgaaa acccaggaag
     1261 attttggttt atctagggta tcggggaatt tttgtaggta aagagctcct aaagcaccaa
     1321 aagatatatg ggaaaaagcc cgaaaaacga taaccatggg atagccagcc atcaaaaatc
     1381 caaaactaga ggctaggatg acaaaaactg ccatcaaggg cgacaagaac atggctataa
     1441 aaatagcgat gtggctcccc aaagtatagg aagcaggtgg aatgacaatc ttgaaaggca
     1501 taacaattgg aatcaaaatc gcaatagccg ttaaaagggc tgtcattgtc ataaattgtg
     1561 tctttttccg tgtattcaca agaatctcct ttttaactgc atatacacta gtatggtaca
     1621 ataaaccaga caataaagca agaatttact tgggtttata gatcattttt tagttaaaag
     1681 ttatagtaga tggaaactag aatagtccac ctctacttct aaaacattgt tagcgatttg
     1741 actgtcctga tcgatttgtc ctgttcttat ttcgttttac tatagtaaag atttcattaa
     1801 aaagaaactg tatagagcaa aatctccacc ttcaggtttg aaagcggaga ttgtttttat
     1861 tttttcaagg tttgtagtcg tggaacaatg gctagggtta gaattgcaga aatgaccaat
     1921 tcagcaattg aatttgttga gataacggtt gccagaagaa gttggatatt tccattataa
     1981 acatttccaa ataggaagaa gattcctcca aggacaaaga tagtatttgt caaggaacca
     2041 agggctccag ctaaaatcag accagtcttg tttttcatca gtttatagac taagtaagga
     2101 gttaaaccaa tcaaaatacg tgggacgatg gcaatgatag ctgagtagat gtttccgttt
     2161 ggtacgaagg gagagaagag gtagcttgtc ggtagaatcg taatcgtgtt aaccgtcaag
     2221 ctaagtaatc ccatcaaaaa tccaagtgta accccaactc gtggaccata aataatgctg
     2281 gcaataatga caggaatatg aacaatggtc ggtttgattg gaaatggaaa aaggttaaag
     2341 ataagtgagc tcagaaagtg tatcacgagc atggtagcaa aaaagatagc aatgggtgca
     2401 atattagagc gttttttcat cgatagtttc ctttattctt tctaaaataa ttgtgaggtc
     2461 agctaaagct cctcgtccgt ggtctccaca agctagtagg gattccttag gagcaatcag
     2521 ctgatagccg taggtttcta atgttttcag attattctga gttactggat ggtcatacat
     2581 ttttgtattc atagcaggag ctattagttt gggaatatga cttggtaggg ctagagctgt
     2641 actggttacc atgttgtccg caaatccgtg agctagtttt gcaatagtgt tagcagttgc
     2701 aggtaccacg ataaataaat ctgctttttt tccaagttcg atatgattga cctgatcagg
     2761 atagggttcc ttcatgacat ccaagtggac aggattctgt gagagtacct gtagtgtcaa
     2821 aggttggata aactctgtag cagcctgagt cattaagaca gtgacttgat ggccttgttt
     2881 ttttagagaa ctgactaaat ctgccgactt ataagaggcg attgaacccg ttacagccaa
     2941 gagaatgttt gccatagttt tcctttctaa gaatgatagg cttgaatttt ttcaaggagg
     3001 agttctgcaa tttcttcttt agtctggact gtttgaagct gatttttctc aacaaatata
     3061 gctcggtgct gatctgctga aatttgagtc aggtcattcg cgatgattaa atctgcttga
     3121 ttcttgataa gactttttcg tgcaatgtca accagatgat cttcggtaac atcaaccagc
     3181 agtttgaaac caatcagatg aatagtggga ttccattcct tgactaggga tatgattttg
     3241 ggtgtctttt taaggaacaa aacctgaacc tcatcagttg aagaaatctt ggcctgatga
     3301 ttctgcttgc ttaaaaattc ttttagattg gagctagcct gaacttcctc aagccctgtc
     3361 atataaacag gagtgtagtc agaaacagcc attgagtgga tcaagacctg ataatcctga
     3421 acacgttctt gcatttctat tagaaggtcc ttggtattgg taatttctcg aatacttagg
     3481 ttaggatgag gctctggctt cagagctcgt tttgtcgtaa ttaaacaaac ttcatgccct
     3541 gcagaaagca aagtctctgt gataattttc cccaagtgac ctgtagaatg gttagtgata
     3601 gagcggacgc tatcgatagc ttcactggta ccgcccgatg taactaaaat tttcatagca
     3661 ctattgtaca caaaaataag cggtaagtaa aatgaaagtg ataaattttt ggtaaaaacg
     3721 aagatttact atagtttcaa actataaagc catataaaat ttaagaaagg actctaaaag
     3781 ccttaaaaac agagatattt acgaacgttt agagagtttt aggatgttaa aaatcttgtt
     3841 ttgtgatata atgaattatc atataagagg ttagaggaat tttgaatgaa aacagatatt
     3901 gaaatcgcac agagtattga gttgaagcca attgttgatg ttgtagagaa acttggtatt
     3961 tcttatgacg atttggagtt gtacggaaag tacaaggcta aactcagctt tgataaaatt
     4021 cgtgcagttg agagcaatcc agtcggtaaa ttgatcttgg ttactgccat caacccaaca
     4081 cctgcaggtg aaggaaagtc gacgcttacc attggtcttg cggatgcctt gaacaagatt
     4141 ggcaagaaaa ccatgattgc tatccgcgaa ccgtctcttg gtccagtcat ggggatcaag
     4201 ggtggtgctg ctggtggtgg gtatgcacaa gttctgccaa tggaagacat caacctccac
     4261 tttactggag atatgcatgc tattacaact gccaacaatg ccctttctgc cttgattgac
     4321 aaccacttgc accaagggaa tgagctggaa attgatcaac gtcgtatcct ctggaaacgc
     4381 gttgtggact tgaacgaccg tgcgctccgc catgtgactg ttggtcttgg tggtcctcta
     4441 aacggtattc cacgtgagga tggttttgat attacagttg cttcagaaat catggcaatt
     4501 ctttgcttgg caacggacat cgaggacttg aaacgtcgtt tggcgaatat cgttattggt
     4561 tatcgctatg accgtacgcc tgtttctgta ggtgatttgc aggttgaggg tgccttggct
     4621 ttgattttga aggatgctat taagccaaac ttggttcaga caatttacgg tacacctgcc
     4681 tttgtacacg gtggtccatt tgccaatatc gctcatggct gtaactctgt tttggcgacg
     4741 acaacagccc ttcacttggc tgattacact gttactgaag ctggttttgg tgcggacctt
     4801 ggtgctgaga aattccttga tatcaagaca ccaaacttgc caacatctcc agatgcagtt
     4861 gtcattgtcg caacccttcg tgcccttaag atgaatggtg gtgtggctaa agacgctctg
     4921 actgaagaaa atgtagaggc agttcgtgca ggttttgcta acttgaaacg ccacgttgaa
     4981 aatatccgta agttcggtat tccagcagtt gtagctatta acgaatttgt atctgataca
     5041 gaagctgaaa ttgcagtctt aaaagaactc tgtgcctcaa tcgatgtacc agttgaattg
     5101 gctagtgtct gggctgatgg agcagaaggt ggagtagcac ttgccgaaac agttgttaag
     5161 acaattgctg aaaatccagc taactataaa cgtttgtacg ataatgccct ttctgtccaa
     5221 gaaaagattg aaaaaattgt cactgaaatc tatcgtggta gcaaagtgaa ctttgagaag
     5281 aaagctcaaa cacaaattgc tcaaatcgtt caaaacggtt gggacaaatt gccaatctgt
     5341 atggctaaaa ctcaatacag tttctcagac aatccaaatg cgcttggagc accagaaaac
     5401 tttgaaatta ccattcgtga attggtacca aaattaggtg caggcttcat cgttgcctta
     5461 actggtgatg ttatgaccat gccaggtctt ccaaaacgtc cagcagctct caacatggat
     5521 gttgaaagcg atggaactgt actaggcttg ttctagtgga ttgaaattga ctttaagtga
     5581 gtttggaaat aatatccaag ctcatttttt ctcagactgc aagcagttca tcaataagat
     5641 ttatactctt cgaaaatctc ttcaaactac gtcagcttca ccttgccgta ggtatatgat
     5701 actgactctg tcagttctat ccacaacctc aaaacagtgt tttgagcaac ctgcggctag
     5761 cttcctagtt tgctctttga ttttcattga gtataagtag atagaaaagt tatatactat
     5821 ctattgaaaa tattataaat cagatacaaa gacaggtgaa accttctgtg ttatactaga
     5881 gatatgttag atttgaaaga atacggtatc gtcatgtggc cggaggagaa agtcatttct
     5941 ttccgtgaga aacttctcgc ttggtatgat gaaaacaaaa gagatttgcc ttggaggaga
     6001 agtaaaaatc cttatcacat ctgggtatct gaaatcatgc ttcagcagac cagggtggat
     6061 acagttatcc cttactacga aagattcctg gactggtttc caactgtcga aagtctggca
     6121 actgcgcctg aggagagttt actgaaagct tgggagggct tgggctatta ttctcgagtt
     6181 cgcaatatgc aggctgcagc ccagcagatt atgactgact ttggtggcca atttccaaat
     6241 acctatgaag gaatttccag cttgaaaggg attggacctt acacagcagg agccatttcc
     6301 agtattgctt ttaacttgcc tgagccagct gtagatggta atgtcatgcg ggtcttggcg
     6361 cgtctgtttg aagtcaacca cgatattggg attccaagta atcgaaaaat ttttcaggca
     6421 atgatggaga tcttgattaa tccggatcgt ccgggtgatt ttaatcaagc cttgatggac
     6481 ttagggtctg atattgagtc tcctgtaaat cccagacctg aagaaagccc agtcaaggac
     6541 tttagtgcgg catatcagaa tggaaccatg gaccgttatc caattaaatc tcctaagaaa
     6601 aagccggttc ctatttatct taaagcctta gttgtgaaaa attctcaagg acaattttta
     6661 cttgaaaaaa atgaaagcga aaagctcttg gcaggttttt ggcatttccc ctttatagaa
     6721 gttgataact tttcgcaaga agagcagttt gacctctttc atcaggttgc ggaagaaagt
     6781 gtgaactttg gccccagtcc agaagagagt ttccagcagg actatgacct agatgttgat
     6841 tggcttgatg tttgttttga tacagtccag catgttttta gtcatcgaaa atggcatgta
     6901 cagattgtag caggtcaggt gagtgacttc catgattttt cagataggga agttcgttgg
     6961 ctttcaccag aagaattcaa gaattaccca cttgccaaac cccaacaaaa aatctggcag
     7021 gcttatgcac aagccaactt agacagtagc caagactagc tattgtgtct tctgactatt
     7081 ttttggtata atagtagaga aaaaggtgaa catatgaaaa aaatactaat tgtagatgat
     7141 gagaaaccaa tctcggatat tatcaagttt aatatgacca aggaaggtta cgaagttgta
     7201 actgctttta atggtcgtga agcgctagag caatttgaag cagagcaacc agatattatt
     7261 attctggatt tgatgcttcc agaaattgat ggtttagaag ttgctaagac cattcgtaag
     7321 acaagcagtg tgcccattct tatgctttca gccaaagata gtgaatttga taaggttatc
     7381 ggtttggaac ttggggcaga tgactatgta acgaaaccct tctccaatcg tgagttgcag
     7441 gcgcgtgtta aagctcttct gcgtcgttct caacctatgc cagtagatgg tcaggaagca
     7501 gatagtaaac ctcaacctat ccaaattggg gatttagaaa ttgttccaga cgcctacgtg
     7561 gctaaaaaat atggcgaaga actagactta acccatcgtg aatttgagct tttgtatcat
     7621 ttagcatcgc atacaggtca agtcatcacg cgcgaacact tgcttgagac tgtctggggt
     7681 tatgactatt ttggtgatgt ccgtacagtt gatgtgactg tacgacgtct gcgtgagaag
     7741 attgaagata cgcccagccg accagagtat atcttgacgc gccgtggtgt agggtattac
     7801 atgagaaata atgcttgatt tactgaaaca aaccattttt accagagatt ttatctttat
     7861 cctgattttg ttaggtttca tccttgttgt gaccctctta ttactggaaa atagacgtga
     7921 taatattcag ttgaagcaaa tcaatcaaaa ggttaaagat ttgattgcag gagattattc
     7981 caaggttctt gatatgcaag gtgggtctga aatcaccaat attaccaata atttgaatga
     8041 cttgtcggag gttattcgtc tcactcagga aaatctagaa caagagagta agaggctaaa
     8101 tagtattctg ttttatatga cagatggggt tcttgcgacc aaccgtcggg gtcagattat
     8161 catgattaac gatacagcca agaagcaact ggggttggtt aaggaagatg ttctgaatag
     8221 aagcattttg gaattgctca agatagaaga aaactatgaa ttgcgtgatt tgattaccca
     8281 aagtccagaa ttgttgctag attcccaaga tatcaatggc gaatatttga accttcgagt
     8341 tcgctttgcc ttgatacgtc gagagtctgg ctttatttca ggtttggtgg ctgttttgca
     8401 tgatacgacg gagcaggaga aggaagaacg cgaacgaaga ctctttgttt ccaatgttag
     8461 ccatgagtta cggactcctc tgactagcgt aaaatcctat cttgaagcct tggatgaggg
     8521 ggctttgtgt gaaactgtag caccagactt tatcaaggtt tctcttgatg agaccaaccg
     8581 tatgatgcgc atggtgacgg atctcctcca tctttcacgt attgataatg ctaccagtca
     8641 cctagatgtg gaactgatta acttcactgc ttttattacc tttatcctca atcgttttga
     8701 caagatgaaa ggacaggaaa aggagaaaaa atatgagttg gtgagagatt atcccatcaa
     8761 ttctatctgg atggaaattg atacagataa gatgacgcag gttgtcgaca atattttaaa
     8821 taatgctatt aagtattcgc cagatggggg taaaatcact gtcagaatga agacaactga
     8881 agaccagatg attttatcca tttctgacca cggtttgggg attcctaagc aggatttacc
     8941 acgtatcttt gaccgtttct atcgtgtgga tcgtgctaga agtcgtgcac aaggtggtac
     9001 aggtctagga ctgtctatcg ctaaagaaat tatcaaacaa cataagggct ttatttgggc
     9061 caagagtgaa tacggcaagg gttcaacctt taccattgtg ctcccttatg ataaggatgc
     9121 agtgaaagaa gaagtatggg aggatgaagt agaagactag aatgagtgaa ataggcttta
     9181 aatacagtat tttagcgtcg ggttccagtg gaaattcttt ttatctggaa acctcaaaaa
     9241 agaagctttt agtagatgca ggcttgtctg gcaagaaaat taccagtctg ctagctgaaa
     9301 ttaaccgtaa gccagaagac ctggatgcca tcttgattac ccatgagcat tcagatcata
     9361 tccatggagt aggcgttttg gctcgcaagt atggtatgga tctttatgcc aatgaaaaga
     9421 cctggcaagc tatggaaaat agtaaatatc ttggcaaggt ggattcttcg caaaagcata
     9481 tttttgaaat gggtaaaacc aaaacctttg gagatatcga catcgagagt tttggtgtaa
     9541 gccatgatgc agtcgcaccg cagttctatc gctttatgaa ggatgataag agttttgtcc
     9601 ttttgacaga tacaggttat gtcagtgacc gtatggcggg cattgtcgaa aatgcggatg
     9661 gctatcttat cgaggccaac catgatgtag agattttgcg atcaggttct tacgcttggc
     9721 gactcaaaca acgaatccta tctgaccttg gtcacctttc taacgaggac ggtgctgaag
     9781 ctatgattcg gacgctagga aatcgtacta agaagattta ccttgggcat ttatctaagg
     9841 aaaataatat caaggaactg gctcatatga ccatggtcaa tcagctggct caagctgatc
     9901 tgggagtcgg agtagacttt aaggtttatg atacctcacc agataccgca acaccattga
     9961 cagagatata gaaagaacgc tgagaaggtg ttctttttat attgactgaa cacctaaaaa
    10021 gtaatacaat ggtgttacca ttaaaaaagg gagcacaaaa gatgactact attacattaa
    10081 aagtttctga agctgataaa acatttatga aagcaatggc taagtttgaa ggagtttccc
    10141 tttcggaact tattcgcacc aaaactcttg aagccctaga agatgaatac gatgctcgtg
    10201 tggcagattt agcctatcaa gagtatttag aagacttgga aaaaggagtt gaacccatta
    10261 cttgggaaga aatgatgcat gatttaggct tgaaggatga ataatttgta taaattagtt
    10321 ccaacaagac gttttatcaa gcaattgaaa aaattggacc gttatacgca gaagctaatt
    10381 acaaactatt tacaaaccaa tgttttggaa ggtccaagac gacacggaaa ggctttggtt
    10441 ggtaatcgcg ttggtcaatg gcgctataga attggtaatt atcgagttat cgtacaaatt
    10501 gtagatgatg aattagtcgt tgctactcta gaagttggtc atcggagaga tatttattga
    10561 attaaagctg agtaataaca atatagaaag taaggagagt aaaaatttga agaaaaaatg
    10621 gttatttgca gattattatg atacaacaat tattttactt gctttaatct ctgttattct
    10681 ggtattactt ggatttgctg agatgactga tttggacaat ccgccttata gtattattga
    10741 tttagtaatc tggggtgttt ttgtaattga ttatagttgg cgctttttta ttactaaaag
    10801 aaaatggcgc tttattcttg aaaatatttt tgatttattg accattctcc ctttaaatgc
    10861 tatttttaca gtgtttcgat taggacgtat cttccgctta gcaaggctga caaaattact
    10921 gaaactgact cgtttactta gaataattgg acttacaggt aaactagaaa gaaaaattag
    10981 tagattctta cgaacaaatg gattgattta tatcttgtat gttaatatct ttattgttct
    11041 agtagggagt tcgattttat ccgttgttga agaaaaatcc ttctcagata gtctttggtg
    11101 ggctcttgtg acagtaacta ctgttggtta tggtgatatt gttccagttt ccctttttgg
    11161 gaaatggcta gcagttttac tgatgcttgt cggtattgga acaataggga tgctaacaag
    11221 tgctttgacg aacttttttg taaaggataa tccagatgaa cagataaaac ttgataaact
    11281 caaagacgaa ttatcgagct aaagaatatt aatagagaaa caatctgaaa aaatcgaaga
    11341 actacattga atgataaaag acttagttga aaaaatatca taaaggtgag acaaaataaa
    11401 acttgattcg aggagaaatc aagtaagttt tgacacaata gaacgcatat tatcaggtat
    11461 aaaaacttga tactatgcgt tttatagaga aataaaataa aatctgaaca aatcaattag
    11521 gaatttaaag cattttataa caacgtttta gagtaatggg gtgctatttc aacttcaacc
    11581 tactataata cagaaaaaaa tacagaaaaa aacaactccc tgatgattca aggagttgtc
    11641 tatagttaaa ttagttttta gaagcttctt ggaattctgg atttttccat gcttcgtcaa
    11701 taatagcttg caattcttta gcagatgctt gcattttttg agtttctgca tcgttcaatg
    11761 gaatgtttac tggacgaacg ataccatgtg caccaacaac agctggttga ccgataaaga
    11821 cattctcaac tccgtattga ccttcttgga atactgaaag tggaagtact gcgttttcat
    11881 cgtcaaggat tgctttagtg atacgagcaa gggctactgc gataccgtag tatgttgcac
    11941 cttttttgtt gatgattgtg taggctgcat cacgaacacc ttcgaacaat tcaatcaatt
    12001 cagcttcttg aacattttga gtgtctttaa ggaattcttc aaggtttaca ccagcgatgt
    12061 tagcgtgtga ccaaacagcg aactcagagt caccgtgttc acccatgatg taggcgtgca
    12121 ctgaacgagc atccacatcc aatttttcag caagtgcttg acggaaacga gctgagtcaa
    12181 gtgaagtacc tgaaccgata acgcgttctt tagggaaacc agagaatttc caagttgagt
    12241 aagtcaaaac gtcaactggg ttagcagcaa caaggaagat acctttgaaa ccagattcaa
    12301 caacttgagt tacgattgat ttgttgatag caaggttttt acctacaagg tcaagacgag
    12361 tttcacctgg tttttgaggt gcacctgcag tgatcacaac aaggtcagcg tctgcacagt
    12421 cagagtattg agctgcatag atttttttag gtgaagtgaa ggcaagggcg tgactaaggt
    12481 caagcgcatc accaacagct ttttcatgca attgtggaat ttcgataatt ccaagctctt
    12541 gtgcaattcc ttggttaaca agtgcaaaag cgtaagatga acctacagca ccatcaccga
    12601 caaggataac ttttttgtgt tgtttagttg aagtcattgt tttaaacatc tccttaattt
    12661 tattagggga ttttccctag acaacttcat tctatcactt ttaaaaaact ttgtcacgaa
    12721 tatgccttat agttctcgat gtaaacgttt tagtggttta gaggctgaaa tagatgggaa
    12781 tttatggtat aatgttgtta cttactaatt gtgaaatgag gcatttatta atgcaggata
    12841 aaaatttagt gaatgtcaat ctgacaaagg agatgaaggc aagttttatc gactacgcca
    12901 tgagtgttat cgtagcgcga gctcttcctg atgttcgaga tggcttaaaa cctgttcacc
    12961 gtcgcattct ctacggaatg aatgaattgg gtgtgacccc agacaaaccc cataaaaaat
    13021 ctgctcgtat tacaggggat gtcatgggta aatatcaccc acacggggat tcctctattt
    13081 atgaagccat ggtccgtatg gctcaatggt ggagctaccg ttacatgctt gtagatggtc
    13141 atgggaattt tggttccatg gatggagata gtgctgccgc tcaacgttat accgaggcac
    13201 gtatgagcaa gattgctctg gaaatgcttc gtgatatcaa caaaaataca gttgatttcg
    13261 ttgataacta tgatgccaat gaacgggaac ccttggtctt gccagcgcgt tttccaaacc
    13321 ttttggttaa tggagcaact ggtatcgcgg ttgggatggc aaccaatatt ccacctcata
    13381 atctgggtga aaccattgat gcagtgaagt tggtcatgga taatcctgaa gtgactacca
    13441 aggacttgat ggaagtcttg cctggaccag attttccaac tggtgctctt gtcatgggga
    13501 aatcaggtat ccataaggct tatgaaacag gtaaaggttc gattgtccta cgttctcgta
    13561 cagagattga aacgactaag actggtcgtg agcgtatcgt tgtaacagaa tttccttaca
    13621 tggtcaataa aaccaaggtg catgagcata ttgttcgctt ggttcaggaa aaacgcattg
    13681 agggtatcac agcagtacgt gatgagtcaa accgtgaagg tgttcgattt gttattgaag
    13741 tcaagcgcga cgcctcagcc aatgttattc tcaataacct cttcaaaatg acccaaatgc
    13801 aaaccaattt tggattcaat atgctcgcta tccaaaatgg tataccgaaa attttgtctc
    13861 ttcgtcagat tttggatgct tatattgagc accaaaaaga agtggttgtt cgtcgtacac
    13921 gttttgataa ggaaaaagcg gaagcgcgcg ctcatatctt agaaggtctc ttgattgcgc
    13981 tagaccatat cgacgaagtg attcgtatca tccgtgctag tgaaacggat gcggaagctc
    14041 aagctgagtt gatgagcaag tttaagcttt ctgaacgtca aagtcaagct atccttgata
    14101 tgcgtcttcg tcgtttgaca ggtttggaac gcgataagat tcaatctgag tatgatgacc
    14161 tcttggctct gattgcggat ttagcagata ttcttgctaa gcctgaacgt gtttctcaaa
    14221 ttatcaaaga cgaattggat gaagttaaac gtaaattttc tgataaacgc cgtacagagt
    14281 tgatggttgg acagatcttg agtctcgagg atgaggactt gattgaagaa tcggatgtct
    14341 tgattaccct ttctaacaga ggctacatta agcgtttgga tcaggacgag ttcactgctc
    14401 aaaaacgtgg gggtcgtggt gtccaaggaa cgggagtgaa agatgatgac tttgttcgtg
    14461 agttagtgtc aactagcacc catgatcatc tgctcttctt cacaaacaag ggacgtgtct
    14521 atcgtcttaa aggttatgaa attcctgagt atggtcggac tgccaaaggg ctaccagtag
    14581 tcaatctctt gaaattggat gaagatgaaa gtattcagac ggttatcaat gttgagtctg
    14641 atcgcagtga tgatgcttat ctcttcttta caacccgtca cggtattgtg aagagaacta
    14701 gtgttaagga gtttgccaat attcgtcaaa atggtctcaa agcgctgaat ttaaaggatg
    14761 aagatgagtt aatcaatgtc ttgttgacag aaggagatat ggatattatc attggtacca
    14821 agtttggtta tgcagttcgc tttaatcaat cagccgttcg tggtatgagc cgtatcgcca
    14881 ctggtgtgaa aggtgttaac cttcgtgaag gagacacagt tgttggtgcc agcttgatta
    14941 ctgatcaaga tgaggttctt attatcacag aaaaaggata tggtaagcgt acagtcgcta
    15001 ctgaataccc aacaaaaggt cgtggtggta agggaatgca gacagctaaa attaccgaaa
    15061 aaaatggctt gctggccggt cttatgactg ttcaagggga tgaggatttg atgattatca
    15121 ctgatacagg tgtcatgatt cgaaccaatc ttgccaatat ttcacaaaca ggacgtgcaa
    15181 ctatgggagt taaagtaatg cgcctggatc aagatgctca gatagtgact ttcacaacgg
    15241 ttgcggtggc agaaaaagaa gaagttggga cagaaaacga aacagaaggt gaagcataat
    15301 gtctcaaaaa aataataaaa agaaaaacaa gcgaaaaaat ctgctgacaa atatcctagc
    15361 aggatttctg atattactgt cactggcttt gatttttaat actcaaattc gaaatatttt
    15421 catagtctgg aataccaata agtatcaagt tagccaggta tcaaaagaaa aattagaaga
    15481 aaatcaggat acagaaggca attttgactt tgattctgtc aaagctatct cttcggaagc
    15541 tgttctaact tctcaatggg atgctcaaaa attaccagtt attgggggaa ttgcaattcc
    15601 tgaattggaa atgaatttgc cgatttttaa aggacttgat aatgttaatc tcttctacgg
    15661 agctggtaca atgaaacgcg agcaagtaat gggagaagga aattatagtc tagctagtca
    15721 ccatatcttt ggtgttgata atgctaataa aatgttattt tctcctttag ataatgctaa
    15781 aaatggcatg aagatttatc taaccgataa aaataaagtt tatacttatg aaatacgtga
    15841 agtcaaacgt gtgacaccgg atcgtgttga tgaagttgat gatagagatg gggtcaatga
    15901 aatcacatta gtaacctgtg aagaccttgc tgctacagaa cgtattattg tcaaaggtga
    15961 tttgaaagaa acaaaagatt attcacaaac atctgatgaa attctaacag ctttcaatca
    16021 accatataaa caattttatt aatacaaatc agtgaaatca tggatttcac tgatttttta
    16081 a
//
LOCUS       DAVLFB010000041        15996 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLFB010000041 DAVLFB010000000
VERSION     DAVLFB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.041"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..1470
                     /locus_tag="VXD58_001161"
     CDS             <1..1470
                     /locus_tag="VXD58_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV0675871.1"
                     /translation="SAMVTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQ
                     NHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKS
                     PNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDG
                     AIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSI
                     NSRQNLSVNYDSGVSGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKD
                     YVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETD
                     EKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEA
                     KIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSY
                     DEVETLETLKKAFELTNNEQKDQAVADDNEGDENDDADY"
     gene            1457..2773
                     /locus_tag="VXD58_001162"
     CDS             1457..2773
                     /locus_tag="VXD58_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV0675872.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFFHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            2948..3934
                     /locus_tag="VXD58_001163"
     CDS             2948..3934
                     /locus_tag="VXD58_001163"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV0675873.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCVKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            3998..4069
                     /locus_tag="VXD58_001164"
     tRNA            3998..4069
                     /locus_tag="VXD58_001164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4031..4033,aa:Arg,seq:ccg)
     gene            4252..4950
                     /locus_tag="VXD58_001165"
     CDS             4252..4950
                     /locus_tag="VXD58_001165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV0675874.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            4941..8480
                     /gene="smc"
                     /locus_tag="VXD58_001166"
     CDS             4941..8480
                     /gene="smc"
                     /locus_tag="VXD58_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV0675875.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSHQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDENAATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8477..9271
                     /locus_tag="VXD58_001167"
     CDS             8477..9271
                     /locus_tag="VXD58_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0675876.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9271..10089
                     /locus_tag="VXD58_001168"
     CDS             9271..10089
                     /locus_tag="VXD58_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0675877.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10093..11370
                     /gene="ftsY"
                     /locus_tag="VXD58_001169"
     CDS             10093..11370
                     /gene="ftsY"
                     /locus_tag="VXD58_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV0675878.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVIEVSQKEVKEFPHSEEGNTEFLETVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11416..12903)
                     /locus_tag="VXD58_001170"
     CDS             complement(11416..12903)
                     /locus_tag="VXD58_001170"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV0675879.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWD
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(13052..13792)
                     /locus_tag="VXD58_001171"
     CDS             complement(13052..13792)
                     /locus_tag="VXD58_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0675880.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(13792..15957)
                     /locus_tag="VXD58_001172"
     CDS             complement(13792..15957)
                     /locus_tag="VXD58_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV0675881.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         4965 a         2877 c         3544 g         4610 t
ORIGIN      
        1 tctgctatgg tgacaaaatt actttgtatc tatactgagg gagatccttt ctttggtgta
       61 aatattaata gtcagaaaga attttggaac cactttgttt ctcagacaaa tcaaggtgga
      121 ccttatctgc aaaatcataa aataattgaa ctagtgtcca aaacttatcc tgagttggag
      181 ccatcgaaat taggaactat gctttttgag tattctaagc tttttatgga aaataaggaa
      241 gacaatagta caatggattc atcaaacaat tttcgtcatc aattaactca atctctatta
      301 aagtctccaa acctcatcct ccgtggtgct cctggcacag gaaagactta tcttgctaaa
      361 gaaattgcca aagaattaac ggatggcaac gaagaccaaa tcggatttgt acaatttcac
      421 ccatcctatg attatacgga ttttgtagaa ggtttaagac cagtatcaaa tggggatgga
      481 gctattgagt ttaggctaca ggacggtatt tttaaagatt tttgtcagaa agcaaaagaa
      541 acccaattga ttggaggaca agataatttt gatgaggctt gggattctta cttagaatat
      601 ataaatgttg ctgaagaaaa agaatatata acaaaaacat cttacttatc tattaatagt
      661 agacaaaatt tgtcagtaaa ttatgatagt ggtgtttcag gatggtcact acctagcaaa
      721 tatgtttacg agttgtataa agataaaaat tataataagc aagaatacta caaaagtggt
      781 ggaaaaactg tcctagaaac attgagaaag agatttggtt tgaaagacta tgtttcccca
      841 acagaaattg atactgataa gaaattcgtc ttcatcatcg atgaaatcaa tcgtggggag
      901 atttctaaga tttttggcga actctttttc tctatcgacc ctggctatcg tggtgaaaaa
      961 ggaagtgttt ctacccaata tgcaaatcta cacgaaactg atgaaaagtt ctatatcccc
     1021 gaaaatgttt acatcatcgg aactatgaat gatattgatc gttcagtgga tacctttgat
     1081 tttgctatgc gtcgtcgttt tcgttttgtt gaagttactg ccgagggtca agttggcatg
     1141 ttggataaag agctgaatat ccatgcagaa gaagcaaaaa ttcgtctaag aaacttgaac
     1201 gctgctatcg aaaatgttca ggaattaaac agtcattatc atattggacc aagttatttt
     1261 cttaagttga aggatgtaga ttttgactat gaattactct ggtctgatta tattaagcct
     1321 ctcctagaag actacttgcg aggttcttat gatgaggttg aaactttgga aactttgaaa
     1381 aaagcatttg aactgacaaa taatgagcaa aaagatcagg cagtagctga tgacaatgaa
     1441 ggtgatgaaa acgatgatgc ggattactga taatcaacac aagattatta aagaaaaatt
     1501 tgttgaagaa tatcctaaac taagcaatct tcttttagac agaaccttgg aaagtctatc
     1561 ccaagatgaa cgtattttca tttttccaaa tgatttgact catactcctg atttggataa
     1621 ggaccaaaag atttttgaaa cagtcaatca gaaaatcaag acagggaacg tgattggttt
     1681 tcttggatat ggtcaggaaa gattaacgat ttcctcacga ttttctgatg agagtaatga
     1741 ccattttttg cattatctct taaacaaggt tctttatatc aatctcacta gtttagatgt
     1801 tgctttgtct cgtgaagaga ggctttatca acttttgatg tatctcttcc ccaagtatct
     1861 acaagctgct attcgaaaag gtctttataa ggaatatcat cgattttttc ataacgacag
     1921 taatgttaag ggagtgattg atgtaagaag ccatctcaag aaaaatcttc ctttcacggg
     1981 aaatattgcc tacgcaacga gagagttcac ctatgataat cccctcatgc agttggtccg
     2041 tcacactatt gaatacatta agaatcagaa aagcattggt caaggggtac tagataatct
     2101 ctcaactagt cgtgaaaacg tatctgaaat cgtgcgtgta acgccctctt ataaactagc
     2161 tgatcgtgct aagattactc ggagaaatca atctaaacct atacgtcatg catactttca
     2221 cgagtacaga aacttacaag aactttgtct gatgatccta aaccaagaaa agcacggttt
     2281 agggtatcaa gatcaaaaaa tctatggtat tctctttgat gttgcctggc tttgggaaga
     2341 gtatgtttac accttgttgc caaaaggttt tgtacatccc agaaataagg ataagacgga
     2401 tggaatttca gtattttctg ttgggaaacg aaaagtatat ccagattttt atgacagaga
     2461 acgaaagatt gttctagatg caaaatataa aaaactggaa ttgactgaaa aaggaatcaa
     2521 ccgtgaggac ttattccagc tgatttccta ttcttatatt ttaaaagctg agaaggctgg
     2581 actgattttt cctagtatgg agcagtcagt aaatagtgaa ataggaaaag tagctggcta
     2641 tggagctcaa ttgaagaagt ggtctattcg aatccctcag aatgcctcat cctatagtgc
     2701 attttgtaaa atgatggaaa attcagaaga aaattttaaa gcgattattg atgaagaagt
     2761 ggggagaaaa taatttcccc tctttctttt atttttaaca caagtaaaac ccgaacgata
     2821 tattttaatt cataaaaaaa aatgacaaaa actgtacttt ggtttataat aaattaagga
     2881 aacgtcggaa aaggcgtagt gatgcgagag cataggtagg tcattacaaa agaaacgaga
     2941 catcgatatg ttaaatgaat ttccaatttt tgattacgaa gatattcaat tgattccaaa
     3001 taaatgtgtc attaaaagcc gtgcagaagc ggatacaagt gtcactttag gaaatcacac
     3061 ctttaaacta cctgttgtgc cagcgaatat gcagacgatt ttggatgaaa atgtagcaga
     3121 gcaactggct aaaggtggtt acttctacat tatgcaccgt tttgatgagg caggacgcat
     3181 tccttttatt aaacgaatgc acaatcaagg gctcattgct tccatatctg tcggtgttaa
     3241 ggactatgag tatgatttcg ttagacagct caagactgat gctcctgagt atatcactat
     3301 tgatattgct catggtcatg cggatagcgt gatttctatg attcaacaca tcaagaaaga
     3361 attgccagat acctttgtca ttgctggaaa tgtgggaaca ccagaagctg tgcgtgaatt
     3421 ggaaaatgct ggtgcggatg ctactaaggt cggaatcggt cctggtaagg tttgtatcac
     3481 caaggttaag actggttttg gtacaggtgg ttggcagttg gctgctctac gctggtgtgt
     3541 caaggctgcg cgtaaaccga ttatcgctga tggaggaatt cgtactcacg gtgatattgc
     3601 caagtctatc cgcttcggtg ctagcatgat catgattggt tccctctttg caggacatat
     3661 tgaaagtcca gggaaaacga ttgaagtcga tggtgaacag ttcaaagaat attatggttc
     3721 agcctcacaa tatcaaaaag gtgcttacaa aaatgtggaa ggcaaacgta tcttgcttcc
     3781 tgctaaaggt catctgcaag acactttaac tgagatggaa caagaccttc aaagtgctat
     3841 ttcttatgca ggtggacgtc aggttgctga ccttaaacac gttgattatg tgatcgtgaa
     3901 aaactccatc tggaatgggg atgcttccca ctaagacggg gtctttgact aaaaaacttg
     3961 ttattagagc aaatttctgt tataataaaa caagtttcca cccttagtgt aatggatatc
     4021 acgtaagatt ccggttcttg agatgggggt tcgattccct cagggtggat gtaaacattc
     4081 taaaaagcct ttaatagggc ttttttcttt acactgctct aaatttaccg ctaaaactga
     4141 gaatctctgt ctaaagaatt tccaaatgct ttccagtccc gttttagagt gactcagggg
     4201 gctttttttg atataataaa aaggactgtt atcagttaga aagaggttgg tatgaaagaa
     4261 ttacaaactg tactaaagaa ccattttgca atcgaattta cagacaaaaa gttactggaa
     4321 actgccttta ctcatacgag ttatgccaat gagcaccgcc tcttaaaaat ttcacataat
     4381 gaacgcttgg aatttttagg agacgctgtt ctacagttat tgatttcaga atatctgtat
     4441 aaaaaatatc ctaaaaagcc tgaaggtgac ctatcaaaac tccgtgctat gattgtccgt
     4501 gaggagagtt tagctggttt tgcgcgtgat tgccagtttg accagtttat caagttgggt
     4561 aaaggggaag aaaaatctgg tggtcgcaat cgtgacacca ttcttggtga tgcctttgaa
     4621 gcctttcttg gtgcccttct tttggataag gatgtggcca aggtcaagga atttatctat
     4681 caagtcatga ttcctaaggt tgaagcaggc gaatttgaga tgattacaga ctataaaacc
     4741 catctccaag agttacttca ggtcaatggt gatgtggcta ttcgttatca ggtgatttct
     4801 gaaacagggc ctgctcacga taaggtcttt gatgtagaag ttcttgttga aggtaagagt
     4861 atcggtcaag gccaaggtcg ttctaagaaa ttagcagagc aggaagctgc caaaaatgcc
     4921 gttgagaagg ggctggattc atgtatttaa aggaaatcga aattcagggg ttcaagtctt
     4981 ttgctgataa gaccaaggtc gtttttgacc aaggtgtgac ggcagttgtt ggacccaatg
     5041 gatctggaaa gtccaatatt acagaaagtc tgcgttgggc tttgggggag tctagtgtca
     5101 agagtctccg tgggggcaag atgccggatg tcatctttgc tggaacagaa agtcgcaaac
     5161 cgctcaatta tgcttctgta gttgtgactc tggataatca tgacggattt atcaaggatg
     5221 caggtcaaga aatcagggtg gaacgccata tctatcgtag tggagatagc gaatacaaga
     5281 ttgacggcaa gaaagtccgt ctgcgtgata ttcatgacct cttcttggat actggattgg
     5341 gacgagattc cttctctatt atttcccaag ggaaggttga ggagattttt aattccaagc
     5401 ctgaggaacg acgagctatt tttgaagaag ctgctggagt tttaaaatac aagactcgca
     5461 gaaaagaaac cgagagtaaa ctgcagcaaa ctcaggataa tctggaccgc ttagaggaca
     5521 ttatctacga gttggataat caaatcaagc ctcttgagaa gcaagctgag aatgcccgta
     5581 agtttttaga cttggaagga caacgtaagg ctatttattt agacgttctg gttgctcaaa
     5641 tcaaggaaaa taaggctgaa ctagagtcga cagaagaaga gttggctcag gttcaagaac
     5701 tcttgatgag ttattaccaa aagcgtgaaa aattagaaga agaaaatcaa actcttaaaa
     5761 agcaacgcca agatttacag gctgaaatgg ccaaagacca aggcagtttg atggatttga
     5821 ccagtctgat tagtgactta gagagaaaat tagccctatc gaaactggaa tctgagcaag
     5881 tagccctcaa tcaacaggaa gcacaagccc gtttggctac tttggaggat aagagaagtt
     5941 cactcagcca agaaaaatct gataaagaaa gctctttagc cctcttggaa gaaaatctag
     6001 tccaaaataa tcaaaaactc aatcgattag aggctgaatt gctagctttt tcagatgatc
     6061 ctgatcagat gattgagctc ttacgtgaac gctttgtggc ccttttacaa gaagaagcag
     6121 atgtctcaaa ccaactgacc cgtattgaga atgagttgga aaatagtcat cagctttctc
     6181 aaaaacaagc agatcaacta gaaaagctga aagaacagtt ggctacagct aaagagaagg
     6241 ctggtcagca aaaggctgag cttgaaactg ccaaggagca ggttcagaaa ttattggccg
     6301 actatcaagt ctgtgctaag gagcaagagg agcagaaaac ttcctatcaa gctcaacaaa
     6361 gtcaactctt tgaccgtctg gatagtctca aaaacaagca ggctagagcg caaagtttgg
     6421 aaaatatcct gagaaatcat agtaactttt atgcaggtgt taagagtgtt ctccaagaga
     6481 aagatcgtct aggtgggatt attggagcag ttagtgagca cctgaccttt gatatgcatt
     6541 atcaaactgc cctagaaatt gcgcttggag cgagtagtca gcacattatt gtagaagatg
     6601 agaatgcggc aaccaaagcc attgatttac tcaaacgaaa cagatctggt cgtgcaacct
     6661 tccttccttt gaccactatc aaggctcgta cgatttctag tcagaaccaa gatactattg
     6721 ctgcaagtcc aggatttctg ggcatggcag atgagctggt tactttcgat actagactgg
     6781 aagccatttt caagaatttg ctagctacaa cggccatttt tgataccgtg gagcatgcgc
     6841 gtgcagcagc gcgccaggtt cgttatcagg ttcgtatggt gacattggac ggaacagagc
     6901 tgcgcacagg tggttcttat gcaggtggag ctaatcgcca gaataacagt attttcatca
     6961 agccagaact ggagcaatta caaaaagaaa ttgctgaaga agaagcaagt ttgcgttcag
     7021 atgaagtgag tttgaagaac ttgcaagatg agctagctag attgacagaa agattagaag
     7081 ccatcaaatc tcagggagag caggctcgta ttcaggagca aggcttgtcc ctcgcctatc
     7141 agcagaccag tcagcaagtt gaagagttag aaactctttg gaaactccaa gaagaggaat
     7201 taaatcgtct ttctgaggga gaatggcaag cggataagga aaaatgccaa gagcgcctta
     7261 ctacaatcac tagtgaaaag aataatctgg aagctgagat tgaagagatt aagtctaata
     7321 aaaacgccat ccaagaacgt tatcaaaact tgcaggaaga gctagctcaa gctcgtctac
     7381 ttaagacaga actgcaaggg caaaaacgtt atgaagttgc tgatattgaa cgcttaggta
     7441 aggaactaga taatcttgat attgagcaag aagaaattca gcgccttctt caagaaaagg
     7501 ttgacaatct tgagaaagtt gatacggatt tgctcagtca acaggctgat gaagccaaaa
     7561 ctcaaaaaac aaatctccaa caaggtttga ttcgcaagca gtttgagttg gatgatattg
     7621 agggtcaact ggatgatatt gctagtcatt tggatcaggc tcgccagcag aatgaagagt
     7681 ggattcgcaa gcaaactcgt gctgaagcca agaaagaaaa ggttagcgag cgcttgcgcc
     7741 atctacaaaa tcaattaaca gaccagtacc agattagcta tactgaagca ctagaaaaag
     7801 cgcatgagtt ggaaaatctc aatctggcag agcaagaggt gcaggattta gagaaggcta
     7861 ttcgctcact gggacctgtc aatttagacg ctattgaaca gtacgaagaa gttcacaacc
     7921 gtctggattt cctaaatagc caacgtgatg atattttgtc tgcgaaaaac ctgcttcttg
     7981 aaacaatcac agagatgaat gatgaggtca aggaacgctt taaatcaacc tttgaggcca
     8041 ttcgtgagtc ctttaaagtg accttcaagc agatgtttgg tggaggtcag gcagacttga
     8101 tattgactga gggcgacctt ttaacagctg gtgtggagat ttctgttcaa cctccaggta
     8161 agaaaatcca gtcgcttaac ctcatgagtg gtggtgaaaa agctctatcg gctcttgcct
     8221 tgcttttctc cattattcgt gtcaagacca ttccttttgt catcttggat gaggtagagg
     8281 ctgcgctaga cgaagccaat gttaaacgtt tcggggatta tctcaaccgc tttgataagg
     8341 acagtcaatt tattgtcgta acccaccgta aggggaccat ggcagcggcc gattctatct
     8401 atggagtgac catgcaagaa tcgggtgttt caaagattgt ttcagttaag ttaaaagatt
     8461 tagaaagtat tgaaggatga caattaaact agtagcaacg gatatggacg gaaccttcct
     8521 agatgggaat ggacgctttg atatggatcg tctcaagtct ctcttggctt cctacaagga
     8581 aaaagggatt tactttgcgg tggcttcggg tcggggattt ctgtctctgg aaaaattatt
     8641 tgctggtgtt cgtgatgaca ttattttcat cgcggaaaat ggcagtttgg tagagtatca
     8701 aggtcaggac ttgtatgaag cgactatgtc tcgtgacttt tatctggcaa cttttgaaaa
     8761 gctgaaaacg tcaccttatg tagatatcaa taaactgctc ttgacgggta agaagggttc
     8821 atatgttcta gatacggttg atgagaccta tttgaaagtg agtcagcatt ataatgaaaa
     8881 tatccaaaaa gtagcgagtt tggaagatat cacagatgac attttcaaat ttacaaccaa
     8941 cttcacagaa gaaacgctgg aagctggtga agcttgggtc aatgataatg tccctggtgt
     9001 caaggctatg acaactggct ttgaatctat tgatattgtt ctggactatg tcgataaggg
     9061 tgtagctatt gttgaattag ctaaaaaact tggtatcaca atggatcagg tcatggcttt
     9121 tggagacaat cttaatgact tacatatgat gcaggttgtg ggacatcctg tagctcctga
     9181 aaatgcacga cctgaaattt tagaattagc aaagactgtg attggtcacc ataaggaacg
     9241 gtcggttata gcttatatgg agggcttata atggcagata taaaattgat tgcattggac
     9301 ttggacggga ccttgctgac tactgataaa aggctgacgg atcgtaccaa ggaaaccttg
     9361 aaagctgcgc gtgatcgtgg tatcaaggtc gtattgacaa ctggtcgtcc cttaaaagcc
     9421 atggatttct ttctccatga gttagggact gacggtcagg aagatgagta taccattact
     9481 tttaatggtg gattagttca gaaaaataca ggagaaatcc ttgataaaac agtcttttca
     9541 tatgatgatg tggcacgttt gtatgaagaa acagagaaat tatcactgcc tctcgatgcc
     9601 atctcagaag gaacagttta tcaaatccaa tcggaccaag aaagtcttta tgccaaattc
     9661 aatccagctt tgacctttgt tccagtggac tttgaagact tatctagtca aatgacctac
     9721 aacaaatgcg tgactgcctt tgctcaagaa cccttggatg cagccattca gaagatttct
     9781 ccagaattgt ttgaccaata tgaaatcttt aaatcacgtg aaatgttgct agaatggtca
     9841 ccaaagaatg ttcataaagc aacaggtttg gcaaaactaa tcagccatct tggaatcgac
     9901 caaagtcaag tgatggcttg tggtgacgag gccaatgacc tctctatgat tgaatgggca
     9961 ggtcttggtg ttgctatgca aaacgctgtt cctgaagtaa aggcagccgc aaatgtagtg
    10021 acgccgatga ccaacgatga ggaagctgtc gcctgggcta tcgaagaata tgtgctaaag
    10081 gagaactaag atatgggatt gtttgaccgt ctattcggaa aaaaagaaga acctaaaatc
    10141 gaagaagttg taaaagaagc tctggaaaat cttgatttgt ctgaagatgt tgatcctacc
    10201 ttcacagaag ttgaggaagt ttctcaggaa gaagcagagg ttgaaattgt tgaacaagct
    10261 gtgttccaag aagaggaaat ccaagacaca gttgaagaaa gtctggattt agagccagtt
    10321 atagaagttt ctcaaaaaga agtcaaagaa tttccacact cagaagaagg gaatactgag
    10381 tttctagaga ctgtagaaga aaataattct gaaattcttg aagcagaaag gcctcaaaca
    10441 gaagaaactg ttcaggaaaa atatgaccgc agtcttaaga aaactcgcac aggtttcggt
    10501 gcccgcttga atgccttctt tgctaacttc cgctctgttg acgaagaatt tttcgaggaa
    10561 ctggaagaac tgttgattat gagtgatgtt ggtgtccaag tcgcttctaa cttaacggag
    10621 gaactacgtt acgaagccaa gcttgaaaat gccaagaaac ctgatgcact tcgtcgtgtc
    10681 atcattgaga aattggttga gctttatgaa aaggatggta gctacgatga aagcatccac
    10741 ttccaagata acttgacagt tatgctcttt gttggtgtga atggtgttgg gaaaacaact
    10801 tctatcggaa aactagctca ccgctacaaa cgagctggta agaaggtcat gctggttgca
    10861 gcagatacct tccgtgcggg tgcagtagct cagctagctg aatggggccg acgagtagat
    10921 gttccagtag taactggacc tgaaaaagct gatccagcca gcgtggtctt tgatggtatg
    10981 gaacgtgccg tggctgaagg tatcgatatt ctcatgattg atactgctgg tcgtctgcaa
    11041 aataaggata accttatggc tgagttggaa aagattggtc gtattatcaa acgtgttgtg
    11101 ccagaagcac cacatgaaac cttcttggca cttgatgcat caacaggtca aaatgcccta
    11161 gtacaggcca aagaattttc gaaaatcaca cctttaacgg gaattgtttt gactaagatt
    11221 gatggaactg ctcgaggagg tgtggttcta gccattcgtg aagaactcaa tattcctgta
    11281 aaattgattg gttttggtga aaaaatcgat gatattggag agtttaactc agaaaacttc
    11341 atgaaaggtc tcttggaagg tttaatctaa tcagaagcaa aaatcctgca aggcataaac
    11401 ttgcaggaaa tttttttatt ctaagcgacc atcttgacga taggcgatat ctggttgcca
    11461 agtccatttg gcaccgaatt tttcaagtag gtcaaagctg gcttgaggtc ccatgcttcc
    11521 agctttatag tcatgaagtg gggcaccatt ttcatcccag agctcttcaa tacggtcaat
    11581 caacttccat gatgcaccaa cttcatccca gtggctaaag ttagttgagt tgttatttag
    11641 gacatcataa atcaatttct cgtatggttc tggagaagca ccagttgcag tcgcatctgt
    11701 acggtaatca agtgagttag gagccaagtt aaattcttct cctacttgct tcccatttag
    11761 gctaagagag aagccttctg ttggttgaat atagatggtc aaaatatttg gagcaagtgg
    11821 ttctccaaag atagaatcca tttgtttaaa gacgatgttg acatgagttc ctttttcagt
    11881 cagtcgttta cctgtacgga aaaagaaagg aacactacgg aatcgatcgc tgtctacaaa
    11941 gaaggcacca gatgtaaagg tttcagttgt tgattctgga ttcacatttg gctcgctacg
    12001 ataagagatg tatttcatgc catcaatctt accagagcgg tattgcccac ggataaagtg
    12061 ttctttgagt tcttcatcag ttggatgata gaggttttta aagaccttaa tcttttcagc
    12121 acgaatctcg tcttttgtga agcttgctgg tttgtccatg gcgaggagcg aaagaagttg
    12181 tagagtgtgg ttttggacca tgtcacggag ggcaccggat tggtcatagt agccaccacg
    12241 ttcttctaca cccaagcgct ccgcaaaggt aatttgaaca ttgtcgataa aatccttgtt
    12301 ccaaacgttt tcaaaaatca agtttgcaaa gcgaactgca aagatgcttt ggatcatttc
    12361 cttaccaaga taatggtcga tacggaaaat ttgttcttcg tcaaatgttg ctaggagttc
    12421 gtcattcaac ttgcttgcag ttgcgtaatc tgtaccaaat ggtttttcaa cgatcaagcg
    12481 ctcaaaacct ttgccatcga caatgttttc agatttgagg tgtttggcaa tggttccaaa
    12541 gaactgaggt gccatagaca agaagaagag cttattgtgt tcagcttggt atttttcatt
    12601 aagctcagct tgtaattgac gcaaagcaat ataatgttcc gaatcattga catcatggct
    12661 ttgatagtag aagtggctag caaattcttg ggcttgctcg gtactatctg ccaaatcaag
    12721 gatggactcg acaactacag attcaaaata ttccttactc caaggtctac gggcagttcc
    12781 aataacggca aagtgcttgg aaagattgcc ggatttatat agtctaaaaa gggaagggta
    12841 gagcttgcgt ttagccaggt ctccactcgc accgaaaatt gtaacaataa ccttagatga
    12901 catctagcta cctaatttct atttttattc ctagtcttgc taggtatgag gaaacctcat
    12961 ttcataaact taattctaac ataattttcc gaaaaatcaa aaattccaat acgaagtaga
    13021 aaaactgcaa ggaaatcctt acagtttgag tttagacgtt taagacctta tctaagaact
    13081 ctttcagacg agggtgttgt gggttatcaa agatttggtc aggtgttccg tcttcaagga
    13141 actcgccatc tgcagtaaag ataacgcggt tggcaacctg acgagcaaat cccatctcat
    13201 gggttacgat aatcatggtc ataccttgct cagccaattc cttcataacg ttaagtacgt
    13261 ctccaaccat ctcagggtca agggcagaag ttggttcatc gaagagcatg atgtctggat
    13321 tcattgctag gccacgagcg atggccacac gttgtttttg accacctgat aggctatctg
    13381 gattggcatt agctttatct gctagtccaa ccttttcaag caactccatt cccaattcct
    13441 cagcttcttc cttagtcatc aacttgtgct caataggagc aaaggtgatg ttgtccaata
    13501 cagacatatg agggaagagg ttgaagtgtt ggaataccat gccgatattt tcacggacgt
    13561 ggtcaacatt ggttgttttt tcagttaaat catagccgtt cacagtgatg tgaccgctag
    13621 tgacttcttc taaaagattg aggctacgga ggaaagttga cttaccagaa cctgaaggac
    13681 cgatgataca aacaacatct ccttcataga acttagtcgt aattcctttt aggacttcat
    13741 tttttccata gtgcttgtgt aaatcattta catcaatttt taattttgcc attaacgaat
    13801 cctcttttct aagcgtttcg ctagtctagt caaaagtgtg ataattacaa gatagaagat
    13861 agcaaggatt gcatacatct tgaaactttg gtagttacga gcaatgataa tcttaccagt
    13921 ttggaagagt tcaaccaaac cgatagcaga tacgatagtt gtatctttaa gagcgataac
    13981 gaattggttg acaaagtttg gcaacatcaa tttagttgct tgtggcaaga taatcttacg
    14041 catggttttt ccataagaga tacccaagct tcggctggct tccatttggc caactggaac
    14101 ggcctgaata ccaccacgaa cgatttcagc gatataagca gccgcattga gtgagagggc
    14161 aatggttcca gctacaaagt cgttaattgg gctttgttgg cctgtgatag actcgatgaa
    14221 gtttggaatt ccccagaaga tgaaggctgc aagaatcatc aatggaatac cacgaataac
    14281 gtcaacgaaa atctcagaga tgacgcgaag agatttgtat gggctaacgc taaacatacc
    14341 gaagataatt ccgatgacaa tggcaatagc aaatgagata agagctagag caagagtgat
    14401 accaagaccg ctaaggagtt gtttgtagtt gttttgaagc aagccccaga gcgttgtttc
    14461 gtcaacagta cttgttgaag cagttgaaga ttcgctagct aggtatttgt caagaatctt
    14521 ttggaattca ccgtttgctt taaggtttgc aagtccgttg ttgaacattt caatcagttc
    14581 tggatttgct ccttttttaa cggcaaaggc tgtttcaccg attggagttc cagagattgg
    14641 agttttcaat ttttgacctt ggctgataga atatttgaga acaggttcat catccataac
    14701 ggcatcaatg gcaccagtgt ttaaactgtc atacattgaa gaaccatcag caaaggtttt
    14761 gattttgtaa ccgtatttgc tttgattttc tgttaggaag gtttgagaag cagttccgtt
    14821 tttaacaccg actgtctttc cttttagatc ttcataagaa gcaatattgc ttgattcttt
    14881 gacaccaaga atggtattag cagtgtagta tgattctgag aagtcaaaag ttgccttacg
    14941 agcatctgtg acagacatcc cagcaatgat accatcggct tgaccagctt ggacagcact
    15001 gatagcagca tcaaaaccag ggttggtgat ttcaatttca aaaccttggt ctttagcgat
    15061 tgccttaatc aattccatat caataccagt gtattggttg cttgaatttt ggaaaacaaa
    15121 aggtgcaaaa gaagaatcgc tggcaatgat atatttagcc ttaacaggaa tagcttttaa
    15181 tcctgcgaga gtagttgtag ttggcactgc tgaagatgat gaagcagtcc atttcttgat
    15241 aattttatca agactaccat cttttttcat ttcagccaag gcttggttaa attcagtaac
    15301 caggtgctcg tatttacttc cttttttcac accgaaagca aaacttccta cagcttcacc
    15361 atccatttca atatggagat cttgaccttg gttaatggca tattcgataa caggtttgtc
    15421 atccatcatg gcatcgatgg caccagcact caagctgttg ttcattaaat caccagtgtc
    15481 aaatgtttta atagtaaagc cgtatttatc tttgattgtt tcaaggaaac gttgagcggc
    15541 agttccgttt ttaacaccaa cggttttgcc agttaattgg tcgtacttgc taattttgtg
    15601 tgactttgta gtagcaatga caacttttgt atcatagtaa gtatcagaca tggtgaagac
    15661 tttttcacgt tctttagtct ttgtcatccc tgccatgata gcgtcggctt gcccagcttg
    15721 aaccgcattg actgctgcgt caaatccagg ataggacatc tgaatgttcc agcctttaat
    15781 ctcagcgact ttgttaataa tgtcaacatc aattccttta taagtttgat ctgaatcttt
    15841 aaactcaaaa ggtgcatagg cggtatcaga aacaatctta atcgtttctg ctttggcaat
    15901 acctaatgag aaaattggga ataaaattag caaaaatgct agaaattttt tcttcatttt
    15961 tagtctcctt ttccgaatat tctctcatta tatcag
//
LOCUS       DAVLFB010000042        15498 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLFB010000042 DAVLFB010000000
VERSION     DAVLFB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.042"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            112..1623
                     /locus_tag="VXD58_001173"
     CDS             112..1623
                     /locus_tag="VXD58_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV0675882.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1645..2976
                     /locus_tag="VXD58_001174"
     CDS             1645..2976
                     /locus_tag="VXD58_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV0675883.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(3381..3671)
                     /locus_tag="VXD58_001175"
     CDS             complement(3381..3671)
                     /locus_tag="VXD58_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675884.1"
                     /translation="MGALGYYEGFVPYVSNQYQNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(3659..3910)
                     /locus_tag="VXD58_001176"
     CDS             complement(3659..3910)
                     /locus_tag="VXD58_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV0675885.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            4114..4191
                     /locus_tag="VXD58_001177"
                     /pseudo
     CDS             4114..4191
                     /locus_tag="VXD58_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4309..5193)
                     /locus_tag="VXD58_001178"
     CDS             complement(4309..5193)
                     /locus_tag="VXD58_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV0675886.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(5338..6489)
                     /locus_tag="VXD58_001179"
     CDS             complement(5338..6489)
                     /locus_tag="VXD58_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV0675887.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVIPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(6635..8401)
                     /locus_tag="VXD58_001180"
     CDS             complement(6635..8401)
                     /locus_tag="VXD58_001180"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV0675888.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(8458..11574)
                     /locus_tag="VXD58_001181"
     CDS             complement(8458..11574)
                     /locus_tag="VXD58_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0675889.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(11584..13878)
                     /locus_tag="VXD58_001182"
     CDS             complement(11584..13878)
                     /locus_tag="VXD58_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0675890.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELCIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(13937..14737)
                     /locus_tag="VXD58_001183"
     CDS             complement(13937..14737)
                     /locus_tag="VXD58_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV0675891.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(14734..15483)
                     /locus_tag="VXD58_001184"
     CDS             complement(14734..15483)
                     /locus_tag="VXD58_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV0675892.1"
                     /translation="MTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDFTQGVLIGS
                     ALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAVATQQLDVL
                     AKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLGGEYVADLF
                     AKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGGMSTIGISL
                     VGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
BASE COUNT         4640 a         3286 c         2554 g         5018 t
ORIGIN      
        1 tttagacttt aatatggtaa aacaaatata aatagaaagc gttttcttaa atgtttattt
       61 aagaaagtag ttggtttttt acactttgtt agacatcagg aggaaaaaca aatgagtgaa
      121 aaagctaaaa aagggtttaa gatgccttca tcttacaccg tattattgat aatcattgct
      181 attatggcag tgctaacttg gtttatccct gcgggggcct ttatagaagg tatttacgag
      241 gctcagcctc aaaatccaca agggatttgg gatgtcctca tggcaccgat tcgggctatg
      301 ctaggtactc atccagagga aggttcgctc attaaagaaa cgagcgcagc gattgatgta
      361 gccttcttca tccttatggt tggtggtttc cttggcattg tcaacaaaac tggtgctctt
      421 gacgtaggga ttgcctctat cgtgaagaag tataagggcc gcgaaaaaat gttaattttg
      481 gtactgatgc ctttgtttgc cctcggtggt acaacttatg gtatggggga agaaacaatg
      541 gccttctatc cactccttgt gccagttatg atggccgttg gttttgatag cctgactggt
      601 gttgcaatta ttttgctcgg ttctcaaatc ggctgtttgg catctactct gaatccattt
      661 gcgacaggta ttgcttcagc gactgcggga gttggtacag gggacggtat cgtacttcgt
      721 ctgattttct gggttacctt gactgctctt agtacttggt ttgtttaccg ttatgcggat
      781 aagattcaaa aagatccgac taagtcactg gtttatagta ctcgcaaaga agatttgaaa
      841 cactttaacg tagaagaatc ttcatctgta gaatctacac ttagcagcaa acaaaaatca
      901 gttctcttct tatttgtgtt gacattcatc ttgatggtat tgagcttcat tccatggaca
      961 gaccttggcg ttaccatttt tgatgacttt aatgcttggt tgactggtct tccagttatt
     1021 ggtaatattg tcggttcatc tacttctgca ctaggtactt ggtacttccc agaaggcgcc
     1081 atgctctttg cctttatggg tatcctgatt ggtgttattt atggtcttaa agaagataag
     1141 attatctctt ccttcatgaa tggtgctgct gacttgctca gtgttgcctt gatcgtagcg
     1201 attgctcgtg gtattcaagt tatcatgaac gacggtatga ttaccgatac aatcctcaac
     1261 tggggtaaag aaggcttgag cggtctatct tcacaagtct ttatcgttgt aacttatatc
     1321 ttctatctac ctatgtcatt cttgatccca tcttcatctg gtcttgccag cgcaactatg
     1381 ggtatcatgg ctccacttgg agaatttgta aatgtccgtc ctagcttgat tatcactgct
     1441 taccaatctg cttcaggtgt cttgaacttg attgcaccaa catctggtat tgtgatggga
     1501 gctcttgcac ttggacgtat caacattggt acttggtgga aattcatggg caaactcgta
     1561 gtcgctatta ttgtagtgac catcgccctt cttctccttg gaaccttcct tccattccta
     1621 taaaatagtg agtgaggtga ttccatgaaa atagatataa caaatcaagt taaagatgaa
     1681 tttcttatat cattaaaaac cttgatttcc tatccttcag tactcaatga aggagaaaat
     1741 ggaacacctt ttggacaagc aatccaagat gtcctagaaa aaactttaga gatttgtcga
     1801 gacataggtt tcactaccta tcttgaccct aaaggttatt acggatatgc agaaatcggt
     1861 cagggagcag agcttctggc cattctctgt catttggatg ttgttccatc aggtgatgaa
     1921 gcagattggc agacaccgcc atttgaagca actatcaaag acggctgggt attcggacgt
     1981 ggtgtccaag atgataaagg cccttcgctc gcagctctct atgcagtaaa aagcttgctg
     2041 gaccaaggta ttcagttcaa aaagcgcgta cgctttatct ttggtaccga tgaggaaacc
     2101 ctctggcgct gcatggcacg ctacaatacc atcgaagaac aggccagtat gggctttgca
     2161 cctgactcat cttttcctct gacctatgct gaaaaagggc ttctacaggt caaacttcat
     2221 ggcccgggat cggatcaact agagcttgaa gtaggaggcg cctttaacgt tgtaccagac
     2281 aaggccaact accaaggtct cctctatgaa caggtttgta acggtctcaa agaagctggt
     2341 tatgattacc aaaccactga acaaaccgta acggttctcg gagtgccaaa gcatgctaag
     2401 gatgctagtc aaggtatcaa tgctgtcatc cgactagcta ccattcttgc tcctctccaa
     2461 gaacaccctg ctctcagttt tcttgcaaca caagcaggtc aagacggcac aggaagacaa
     2521 atctttggtg atatagcaga tgaaccttct ggtcacctat cctttaatgt cgcaggtctc
     2581 atgatcaatc atgaacgttc tgaaatccgt attgacattc ggactcctgt cttagctgac
     2641 aaggaagaac tagtagagtt gcttacaaga tgtgcacaaa actaccaact ccgctacgaa
     2701 gagtttgact atctagcgcc tctatacgtc gcaaaagaca gtaaactcgt tagcacactg
     2761 atgcaaatct accaagaaaa gactggcgat aacagtcctg ctatttcatc cggtggtgcc
     2821 acttttgctc gcaccatgcc aaattgtgta gccttcggcg ccttattccc aggagcgaag
     2881 cagacagaac atcaggcaaa tgaatgtgcc gttctagaag atttgtaccg tgctatggat
     2941 atttatgccg aagccgtcta tcgacttgca acttaatcag gcaactgttt ctacaaaaaa
     3001 aatcgaccga ttaatgaact gcaccccaaa agttagacag aataaatcta acttttgggg
     3061 tgttttatta tgaaattgag ttatgaagat aaagttcaga tctatgaact aagaaagcaa
     3121 ggacaaagct tcaaacagct ttcaaaaaga tttggtgtgg atgtttctgg tctaaagtca
     3181 tctgaatctt tgagatgagc tttataaatt gcttttttca gtttttgcac tggtatttcg
     3241 ataaactcaa actttttagc cgtggtattg cctgatttta tagtatattg aaactagaat
     3301 agtacacctc tacttctaaa acatttttag aaatcgattt gactgtcctg atcgatttgt
     3361 cctgttctta tttcatttta ctatatttga gccacttcgt ctttaacggc tttattcata
     3421 agctcttgta atttttcttt actatcaatt acttctgatt ttccgttgta atttattgta
     3481 ataggtttta acttacctaa tttctcgaca cgctcattaa tttgatcttt tttgaaggct
     3541 gcatatgttt ttcctaagat tttttcgaaa atatatttat cagatagcgg tttgtattct
     3601 tcttcagctt ggttttggta ttgatttgaa acataaggaa caaatccttc atagtaacct
     3661 aatgctccca taagttcaaa agcttgtttt ctaattcaaa ccattgcaac tcagatttca
     3721 gcttttcaga taaatcctgc tcatccaaat aatgacttga aattagtgct gaactcgttt
     3781 ctgtatcctg tacaggctga gcacccatac cagcaaaaaa taaactcgtt cctagcaaga
     3841 ccgaacaagc tcctattgca tatttcctca aagaaaaacg ctgctttctc tcaaattgaa
     3901 attctttcat cccatctccc atcattcatt attactgtat attttgtata tcagaaatag
     3961 tttgtattca caaatctttc tagttattcc cttatcattc ctaattaagg gagataacat
     4021 acaataattt ttagttaaat gtatatcgat gttttttgtt tttcttaata aacgcaatac
     4081 aaaaagagcc tgttgccaag ctctttatac tcaatgaaaa tcaaagagca aactaggaaa
     4141 ctagctacag gttgctcaaa acaccgtttt gaggtagtag ataagactga cgaagtcagc
     4201 tcaaaacact gttttgaggt tgcagataga actgacgaag tcagtaacat ctatacggca
     4261 agacgacgct gacgtagttt gaagagattt tcgaagagta ttagtctatt atttcttctc
     4321 agcgcgaagg gctgacaaga tttgtgtacg gatatcatcc acaccatttg gagtatttgg
     4381 taaaaagatg gtttgatttc ctttagaggc aaaggtattc aaggtatcca aatactggtt
     4441 ggtcaagagg atagacatga tttgttcttc tgtcatgcca acattggctt ccttgagttc
     4501 ggtgatagac tctgccaatc catccacaat cgccttacgt tgttgggcaa tccccacacc
     4561 atgaaggcgg tctttttctg cttcggcttc agctgcagtg acaattttaa tcttgtcagc
     4621 ttccgccaat tcttgtgctg cgacccgctt acgttgcgcc gcattgattt cattcataga
     4681 ttgcttaact tctgcatctg gttcgacctt ggtaatcaag gttttcacga taatgtagcc
     4741 gtaagtggtc atttcttctg ctacttggtg ttgaacctca agggcaatct catctttttt
     4801 ctcaaacaat tcatccaagg ttaattttgg aacagaagag cgaagagcat cttcgatata
     4861 agatttaatc tgagattctg gacgtatgag tttatagtaa gcatctgtca cgctctgctc
     4921 gttgacacgg tactgagtcg ctacattcat cataacgaac acattgtcct tggtcttagt
     4981 ctcaaccaca atatcacttt gcaacaagcg caactgaatc cgtgctgcaa tcgagtcaat
     5041 cccaaaaggc aagcgaatat gaataccgct attagcaacc ttttggtatt tcccaaagcg
     5101 ttcaataatc gccaccgact gctgacgaac cacataaact gtactcagtg tgactatcac
     5161 caataggagc acacaaacaa tcagaaaaat catgaaaaat attgccataa tggaacctcc
     5221 acaagtattt ttctagtatt atagcacatt taaagaaggc tgtgccgttt ttactgcgat
     5281 ttttcctgaa atgtcaataa ttagaggtga attgtcctat tgtcgtccaa tctcttgcta
     5341 aaataactct ttataaaagg caatcgtttc ttctaaggtt ggcataaatg gatttcctgg
     5401 tgcgcaggca tcaatcaagg cattcttaga aaggtattca aagtcgaaat ctttttcttc
     5461 aataccaagt tcagtcagtt tcttaggaat acctactgtc tcagaaagct tctcaatctc
     5521 agcaatcgca taatcggcac attcttgatc tgatttacct tctacatgaa gtcccaaggc
     5581 tttggcaaca ttgcggaaag cttctggtac acgtttagca ttttcacgtt ctataactgg
     5641 tagcaacatg gcacagcaca cgccatgtgg caaattatat actgcaccga gttggtgagc
     5701 cattgagtga acatagccaa gaccagcatt attaaagctc ataccaccaa ggaagatggc
     5761 attgaccata ccttcacgcg cttcaatatc atgtccattt tctacagcac gaggaagata
     5821 ttccttgata agctcaatag caccaataga cagtttcttg gttacatcat aagcaccagg
     5881 tattaccaaa gcttcaacag catgagtcag agcatccata ccagtagcag cagtcaggcc
     5941 tttaggtttg gaaagcatga gttcagggtc attgacagaa atcaaggcaa ggctattctt
     6001 gtcaaccatt accatcttaa ccttgcgttc ttcatcagta atcacatagt taatggtaat
     6061 ttctgctgat gtacctgccg ttgtattgat agccacaact ggcaaacctt ttttagcaga
     6121 cttatgaagc ccttcataat cctgtggttt tccaccattt gtagccatga tagaaataca
     6181 actagctgca tcctgtggag aacccccgcc aagactgatg ataaagtcac agccatgttc
     6241 ttgcaaagca gctaccccat ctgtgacatt tttacaagta gggtttggct ctacatcgct
     6301 aaagatgaca tattcgattc cttctgtatc cagtggtttt aaagtcttag gtaaaatatc
     6361 actgccttcg atgtacttat ctgtcaccaa aagtgccttt ttatagccaa gttccttgat
     6421 ataaggaccc acttcattta caacaccttt tccaataagg ttgactgccg gaacataaaa
     6481 tatagccata taattttcct cctgcgcctc cgcgctatct atttaacatc attatattac
     6541 tttttgtatt cgttttcaat tatttgtgaa ttgtttaaca aatcttttta aaaggatagg
     6601 agggacgtaa gttcacctcc tatataaaca agttttattt atgtagtggt cccaacaact
     6661 gacatgcacg atagtctgct gattctagat cttctgttcc aaataaggac caatttttag
     6721 gtctaaagat atcttcctca ggtacattat gcatattgac aggaattctc aacatagaag
     6781 ccaaggtaat caagtctgct ccaatgtgtc catatgttat ggctccgtga ttagctcccc
     6841 aattattcat gacgtcatag acagacttga aagcaccttt tcctgtcaaa cgtggagcaa
     6901 accaagtagt tggccatcct gaatctgtac gattatctaa agtatggtga acatcttcag
     6961 gaagttcaag tgtgtaacct tctgcaattt gtagcactgg accaacccct tttagaagat
     7021 tgagacgtac cattgttact ggcatatccc ccttcgtcaa gaaacgagtt gagaatcctc
     7081 ctccacggaa gtactcgcgg tttgctggtg ggaagtctgt attttcaagc atagcctgta
     7141 cttcactttc atccaactcc cagaatggtt tcataacagg tttgccatct cgagtagctt
     7201 gacctgtacc atccaatgta caagaaccag agttgattag atgtaagaag ccagctgcag
     7261 cacgaccctc taaagtatat cctgttacac gttcaacagc ctctggactc caataagtac
     7321 gcacatcagc aaagatttgt ggagtatttg ttagtagata attaaagagc atagacacac
     7381 catttagtga atcattctct gtcgcaaata caaatggttt tcgaatacca ttccagtcaa
     7441 actgagtatt gaggaaagtt tccataaaat ctccatttgg aaaatggtct gtccactgac
     7501 gttgaccttg gaaaccagct actaaagcat ggtgaccaac tgcttcttcc tcaaaaccaa
     7561 gttcagcaag tcttgggtta ccaaccatta agtcacgtcc aatcatgaac atcttaataa
     7621 caaattccca ttgtttttct ttctcttctt tagaaagaat caaatcctca cgatttcggt
     7681 caactccttc tttgatatgt tcttttaccc aaaccatagc acgttcaaac tcttcagggt
     7741 cgtaaatacc acggtccata cggcgtgtga actccgtcat atctaccgat tcatttcgca
     7801 ttcctaagta ttcttggaag aaatccggat ttacaataga accaccaatc cccatcgaaa
     7861 cactacccat tgataggtaa gcagtgtctc tcatcaagcc agttgcaaga gctgcacgcg
     7921 cataacgtaa aagtttttct ttgacatctt ctggaatatc tgtatcatta gcttcctgaa
     7981 catctctacc ataaatccca aaggctggaa tccctttttg agcatgtgaa gctagtacag
     8041 ctgcaagata gacagctcct gggcgttctg tcccattaaa tccccaaata gcatgaggaa
     8101 tatctggaga catatccata gtttcactac cataacacca gcatggtgta actgtaattg
     8161 ttgcgcaaac atttgatttt ttaaacaact catgggaagc tgcagcctct ggaacacgac
     8221 caatggtaga tggagaaatc acacattcca caggttcccc atctggatat ttcaatgtgc
     8281 ttgaaatcaa atctgccaca cttttagcca tgttcattgt ttgtacttca agtgattcgc
     8341 gtacaccttg acgacgacca tcaatagtcg gacgaatacc aatacgtgga tgttgaatca
     8401 tactattttc ctcttatttt tctgtttctt tcatactacc gatgataatt tttaaaatta
     8461 cttataagta taaacctcta cctctgccaa acttaaagga attcctgcgt ttctcaaaga
     8521 aattctaacc ttttttccct tcagcccttt taagtctatg gatagattat tgctttctaa
     8581 actgtcaata tgttgtttaa aaacttcatg attatttgaa tcatatataa taatatcaaa
     8641 atttgataat ctctgaattt cagcatctgt tctattataa ataattaact tttctaattc
     8701 ttcggtttgt gctaaatcta tctcccacca tgaaggagaa tctcctttgg tatgagtcac
     8761 tgaatgatga ccataatctc cgtttttatt tccatcaact gctagacgag aaaaaccatt
     8821 gtaatccgtt gaactctgtc gagtttcttt ggttaatgca atattagaaa gtttcttcgg
     8881 agttctcttt gaaccataga cttcaacttc cgctaaactt aacggaatag ctgctgattt
     8941 taattctatt ttgactagct tagcccctac agaaggtact tttaaagata acaaaccatc
     9001 aactacttta tcaaaatgtc ttctaaaaac ttcttctcct gatgaagata gaaaaataac
     9061 atcaaaatta gataaacgct gaggttcggc atctgttcta ttataaatat caactttaga
     9121 aaccgtaaac tcttctccca gatcgacctg ccaccaagcg ttagaatctg ccttagtatg
     9181 agtcaccgaa tgatgtccgt aatctccatc tttattacca tcaactgcta aagcagctac
     9241 accctcataa ttggttgaac tttgagtagc taccttattt gtagagacta ctttatcttc
     9301 tgttatttta tctatatcct cttcagattg cttaccatct gattctctaa aaacctctac
     9361 ttctgctaaa ctcaaaggca ctccactcgt tagtaattta actttaatat accttgctcc
     9421 tttttctttg aaatctagac taacagattc acccgacaaa ttattaacat gtttcttagc
     9481 aacttcgttt ctattattgt catatagaat cacatcaaaa ttagatagac gttgagtctc
     9541 agcatctgtg cgattataaa ttttaacaag cccaacttta tccatttttt tcaaatcgac
     9601 ttcccaccaa gagggattat ctgccctagt gtgtgttacc gaaccagagt taaaattacc
     9661 atttctgtta ccatccacag ctctattagg gtctccattg taatctacag aactttgtgt
     9721 tgttggtttt gcatatgcta tattcaaatt accatcatta aatttatctg gtgttggaaa
     9781 agggactggg cctgtccctt cagtatttat agacatctct accattccat tatgattaac
     9841 cgtaatggta taaacatggg tattctcatc ccaattttct gtataagtat aatgattttt
     9901 atcgccactt atatttatct gaggtttatg accagtatgc ccttttaatg taagtgttgt
     9961 tgtcctaaag tcattatcta caggattgat ggaataattt ttgcttatcc agttcgctaa
    10021 ctctaattct tctttcttcc aactttttga tgcatcaaaa tttcctgata atgcccacat
    10081 agctgtctta tctgtcctgt aattgttcaa taaaatatgt aaattatttg gattttcttt
    10141 aacaacagcg taagtatgtg gcgtcaaatc taacgataac gactttgtat tattagtata
    10201 cataggcaac attacttgct gatttttatt gatattagca ttactattat aaatatacca
    10261 ggaattacct acacgctgag catacccatc tccttcataa agttttggat aaagcgagtt
    10321 taaatagttg actttactag acaattcctc actattttta gtcaaaattt ttgcattagg
    10381 gaatatagat gaaatctttt ccttatcaat tttctcatgt attacaggaa gaatatgata
    10441 tctcccatta ttatataaag gcattgtttc atcattcgaa taaagtcctt gataaaatcc
    10501 gtttaatgaa ctaatcctac cttctccatt ccaaaataca gcttttgttc tatttacaac
    10561 ttcttcctta cttggagctg gattttgtat agcatgtcta aagaaaggaa taataccttt
    10621 agtaaatgct ggagttggta catcatttgt cataaatgta tacgcggcac attcaaaatt
    10681 ataaactgtg cctcccccag tatatacatt catcatttcc atagcaatca ttgattctgg
    10741 ttccgatgca taggatctca tatccctagc tcttccagtt tcaaatgtgt ttgtataatg
    10801 tttttcccac catttccatg tatctgttga tgagccccag ttatcacata agccactcaa
    10861 ccaaaatcca ctaacgatag aatctgtacc cgcatcatct cttattggcg tatttttagt
    10921 tgccaacacc aaatttttat gatatttttg actcgcttca aagaatgtcg gatcattcat
    10981 aatagtttcc cagaaccatt tttcatgatc atgccagata aaatgcgctc catatttggc
    11041 acaaacttcc aaatatttag cactatgcgg agctaactgg ttattgtaaa tccaataatt
    11101 ctcaatattt aaaacacctt ttaacacact atacttttgg aattgttcat ctaaccactc
    11161 tggaggaact gtattacgct ctccagccga cataataacc aagaaaactg gaatgtttag
    11221 actttgagct tcctccaaca tcttgacata ccattctctc aattctttcg tatctcgtgg
    11281 aatacaactt gttggtttac agacttttgc aggatgaagt tcaatcgctg catatggctt
    11341 tacatcttca ggaatagctt cccatgctcc cttcaatgtg ttcccccacc ataatccgtt
    11401 accattatca ttgccataca acggagaaag caacaatggc gattcattat taatcgtcgt
    11461 tctcatttga acacgattat cagccttaac tcctgctaac aatccaaaac ataacatccc
    11521 aatacataca aacaatattg tgattaattt tctttttatt ttttctttat tcatgactta
    11581 tacctaaaaa tttaactcaa taattttttc tgaattaaat accaattcaa tattttgtac
    11641 atcagtattt ttgccatata ttcttactct tactttttga tctttgtttc ctccttccaa
    11701 ttttaggaat gttatatccc catttttcca agcaaacgat accttatatc ctcctcttac
    11761 tctgaaacct ttcacttctc cttctgacca agcagaaggt aaagctggaa ttagtgataa
    11821 ccaattatga tggctctgta ctaataattc acaaattcca ctcaccaaac ctaaattacc
    11881 atcaatttga aatggtggat ggtcaagaaa taaattgcca agagtcgcat tatttaacaa
    11941 accattaatc tggttataag caggttcacc ttgatatagt ctcgcaaaaa aatgaatcag
    12001 ccatgcagca ctccaacctg tttgtgtact agcatgcaaa ccacttacta accaattatt
    12061 aatcgcttgc tccctatcct gtgaagataa aaagttagcg tttgataatc tcctattgat
    12121 agtgatttta gctgcttctg ctaattccgg agttttatga atatcaatct cattataagg
    12181 ataaagccca aatagaggtg aaatgtgtct atgaccaggc tctacttctt cataatcctc
    12241 tagccattct tgtatttgcc cattactacc tatttttgtt ctaggtaatt tcttttttaa
    12301 ctccttcaca cgactaataa aatccgaatt gtctcctaat tgttttgcaa tgccaatgca
    12361 tgaatcacaa aaatatctta gaatttgatt atcaattgta gatgatagac aagcatttcc
    12421 ttcaatacca ttttttaagc gatatttatt ttccggtgag acacttggac ctgtcatcaa
    12481 gtagccatcc acctcaaata aataatcttc aaagaaaaga aatgcttctt ttatcatttc
    12541 aaaatgttcc gtaagaatac gctcatcttg gaaatataaa tagtgttccc aaatatgagt
    12601 acataaccat ggaatagtta atacccaaat tgcagccccc atggcatgag attggggagc
    12661 cgtatcgcca aaaccatccg tattatgatg tgctgtaaaa cctctagctc catacatttt
    12721 cttagcggtt agtcttcccg gttctctcat tctttcgagc atatcaaata atggatattc
    12781 tacttctggt aaatcacatg gacctaccat ccaataattc atttgagtat taatattaat
    12841 cgtatattta gaaccccaaa ttggatttaa ttcatcacac catattcctt gaagattggc
    12901 aggtaaacca ttcggttgac tagacgatat taacagatat cttccataat gaaataacaa
    12961 gttagtcaag tagttactat actttttagt gttttcaaga agtagattcg ttggaatgct
    13021 aagacaacct ttactatagt ctagtttaaa atcaactcta ttaaattgct cctgatattt
    13081 ttttacatgt tcatcttttt ctgtaaagta atcaatacta ctaaattctc cctgaagaga
    13141 agaaatatct atatttcccc aataatccgt cattgatttg agataaagaa atacctctgt
    13201 agcattccga ataactattg tctctcccaa tacacttact tcaccatccg taaccttaga
    13261 atgacatact actttaaact gaacaccttt tctacctcca gcagaggccg acattaaaat
    13321 tgtacttgaa tccagtttag atacttcgtc attaaaccgt ttatttctac ccaaattaat
    13381 gtttaaattt aatgtgtttt gaactgatga cactatacgg caacataaaa tattcttatt
    13441 aaaactcgta aaatattctc tttttatttg taaattacaa ctattaggct caaacacaac
    13501 attagaaata gctgtatcta aatctagctc tctttcatac aatgaaagag cacaagactg
    13561 aatatctata tgctcaatgc aaagttcccc aagtaattca tagtggcttt gatctcttgg
    13621 ggtagcaaac attgttaact ttatcaattc ttcggctttc tgaatttctc catctaaaag
    13681 atattcccga atttttttaa gatgcaatag tgagtctgga ttatttctat ctgactttcc
    13741 tctataccaa atagtctcat cgtttagttg aatacattct tttgtagctg aaccataaat
    13801 cataccacct aaatgaccgt tcccaatcgg caaggcttca ttccaatttg cggcagcttt
    13861 cttataccat aatttcactc tttacctctc ttccaaaatg gaggtgatat cccaaatacc
    13921 acctccacac tttctactac ttcaaaattc ctaaaacaac taggataact ccaatagcca
    13981 taacactaaa gattagtaca accggattct ttccttttct aattaattta cacattaata
    14041 aagtcaaagc catagggata aatcctggaa ttaattttgt aatcatttct tgaacagaaa
    14101 taacaagttc cccctgctta aattctaatc caaaattaat accaaccatt gatggaatca
    14161 aaccacccac tagtactagc cctaatgctg tcgccatact cgtaacgcga ttcaatgttc
    14221 cttttgtatt attttcttgg ataagactag aacccttagt atacccatat ttcaaaccga
    14281 aatatttaac aggaatatta ataatattaa acaatattaa ggcgataaag atacctaaag
    14341 caccgccgtc tttagaataa gacgcaccaa tactaaaaca gataggaact agtgtcagcc
    14401 agaacaaact atctcctaga ccagccagtg gccccatcaa gccaacttta ataccagtaa
    14461 ttgaagctgc accttcattt ccttcttgtt cttccatagc ggaagtaact ccaagaataa
    14521 atggtgccgc tgtttgatga gtattgaaaa actcaaggtg acgtttcatt gcaggagaag
    14581 ctgaatcttt gtctgggtat agttttttca atactggaag aattgtatat agaaaaccta
    14641 ggttctgcat tctctcatag ttccatgaag aaccatacat aaaacttcgt ttgaaaactt
    14701 ttttaagttc aactttagtt actttattat tcatcataga tcaagatcct cctcaacatc
    14761 actacttgaa gttgtttctt gtggtttact tccaatcata tcgtagaagt atgctaccgc
    14821 aataccaact agtgagatcc caatggttga cattcctcca tacacagaac aaatgaatcc
    14881 aatcaacaag aatacccaca tatttttctt gagcatcata tttaaaagca taccaaaacc
    14941 aattgaaggt agtaaagcac ctgcagagtt aagtccctgc ataacgattg gtggaacctt
    15001 agcaaacaag tctgccacat attcccctcc aagcataata gctaggaaaa ttggtacaat
    15061 tttaaataaa gtgattaaaa ccaaacttga ataatgataa aaaccgatct ttgaataatc
    15121 tccgttttta gcatcattat cagctttttt cacaaaataa acatctaaag tttttgcaag
    15181 aacatccaac tgttgagtag caactgcaat tggaactgct atagcgatac cagcagtttc
    15241 tccttgacca gacaaaatac caaatgcagt accaataatc gcacctgaaa tagtatctgg
    15301 tggagtgtaa cctccaatac ctgttacacc gagccaagtt aattcaagag ctgaaccaat
    15361 aagaacacct tgggtgaaat ctcctaagac taagccaacc attgcacttg taactaacgg
    15421 acggtgaatc ataaataacg gtccattata gtcaatagtg gcaataatcc caactaatgt
    15481 caccagtaat gcttgtgt
//
LOCUS       DAVLFB010000043        14614 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLFB010000043 DAVLFB010000000
VERSION     DAVLFB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.043"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..352)
                     /locus_tag="VXD58_001185"
     CDS             complement(<1..352)
                     /locus_tag="VXD58_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675893.1"
                     /translation="MMRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEI"
     gene            complement(358..675)
                     /locus_tag="VXD58_001186"
     CDS             complement(358..675)
                     /locus_tag="VXD58_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675894.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGDWNTFKNNIKGLARASSGFRF
                     AARETGLWAAIWSTRVYSYTYVVSAFAPILGSVAAVNAALAVIGGISVAGITYALWTR
                     KFY"
     gene            complement(820..2172)
                     /locus_tag="VXD58_001187"
     CDS             complement(820..2172)
                     /locus_tag="VXD58_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV0675895.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADNKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLLPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(2169..2636)
                     /locus_tag="VXD58_001188"
     CDS             complement(2169..2636)
                     /locus_tag="VXD58_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0675896.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2629..4848)
                     /locus_tag="VXD58_001189"
     CDS             complement(2629..4848)
                     /locus_tag="VXD58_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEV0675897.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIMEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            5314..6177
                     /locus_tag="VXD58_001190"
     CDS             5314..6177
                     /locus_tag="VXD58_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0675898.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(6466..7857)
                     /gene="argH"
                     /locus_tag="VXD58_001191"
     CDS             complement(6466..7857)
                     /gene="argH"
                     /locus_tag="VXD58_001191"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV0675899.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDVIGSLAH
                     VQMLGQTGILSLEESEKIQVGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGYVLDKLAHLKGVLLDLAENHVATIMPGYTHL
                     QHAQPISFAYHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKQPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGHLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(7886..9083)
                     /locus_tag="VXD58_001192"
                     /pseudo
     CDS             complement(7886..9083)
                     /locus_tag="VXD58_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
     gene            complement(9100..9906)
                     /locus_tag="VXD58_001193"
     CDS             complement(9100..9906)
                     /locus_tag="VXD58_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0675900.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLVTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYSVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(10113..10754)
                     /locus_tag="VXD58_001194"
     CDS             complement(10113..10754)
                     /locus_tag="VXD58_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675901.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(10751..12859)
                     /locus_tag="VXD58_001195"
     CDS             complement(10751..12859)
                     /locus_tag="VXD58_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV0675902.1"
                     /translation="MKKISHLCMLLLLLCTTFFVLNVNVTREIVRIQEMGKTTYSFDL
                     YLKDVTKPTETILQFFEEVASQDKVSIIKVDNGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLFSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(12914..13207)
                     /locus_tag="VXD58_001196"
     CDS             complement(12914..13207)
                     /locus_tag="VXD58_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV0675903.1"
                     /translation="MIGEINHEKISVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            13613..13933
                     /locus_tag="VXD58_001197"
     CDS             13613..13933
                     /locus_tag="VXD58_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0675904.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(13985..14572)
                     /locus_tag="VXD58_001198"
     CDS             complement(13985..14572)
                     /locus_tag="VXD58_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0675905.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT         4579 a         3115 c         2296 g         4624 t
ORIGIN      
        1 caatttcaag aataaaactc catataatcc tagatcttaa atcatcaggc gacaaacttt
       61 tacgatggaa tgaaaaatag ggcgagaacc tcacatttac tttcttcctg cctataaaaa
      121 taaaaggaaa aatcgtaata agagcttctt tcttgtacaa atctttcaat gcgtatagta
      181 tgtctaaaac tatttttgat aaaatcaaac tacagaaagc aagactcata ctaatcacaa
      241 aatcaagggg agattctaga ttcaatagta agttattcac taggacatgc aactctagcc
      301 acaagacaaa aagagggagc attagtaaga aaacaattag tttacgcatt ataacaatta
      361 gtaaaattta cgcgtccata aagcatacgt aattcctgca actgaaatac ctccaattac
      421 agccaatgcc gcattaacag ctgctactga ccctagtatt ggagcgaaag cagaaacaac
      481 ataagtataa gaatacactc ttgtagacca tattgcagcc cataaaccag tttctcttgc
      541 ggcaaaccta aatcctgagg aagctctcgc taaacctttt atattatttt taaatgtgtt
      601 ccagtctccc ccatcaaggt acatcatctc ttcttcatca atcacaacat aattatttgg
      661 taatacaagt tccatttgtt ttctcctttt tattaagcat ggttaggatg gtttcctaac
      721 aagtaatcgt cgtctgtgtt tctatcactt agctacctag ataagatatc ctttctctac
      781 cttatttgtt tttataggtt acctaaggtt taaaaccaat tattgcttga aacttaacat
      841 ttctaaaatc caatcaagat aggtttcttt atcatagaca atccgcactt caactggcat
      901 atccttttgg aggacgactg tctcacttcc atgttttaga gttaaggttt ctaattctat
      961 catgacttta taaagactaa tattgccttc tttcgtttct tgggaaattg ttcctgaatc
     1021 aatctgtctg acttgtccct ttagtgttcc atatttttga ctatttacac cagtaatagc
     1081 aacatcagct tttgctcctt tcgaaacacg agaaatatca ctcgcaagta caaaagcttc
     1141 tacatagtaa cctttttctt tccctgaaac ttctgctatc gtttgcccct gctgaataga
     1201 cattccttgt ttcaaaggta acagataatg cagagtacct tcatttggcg ctaatatact
     1261 atgagcttta tcttgacctg ttgccactcc aagattagcc tcaagctctg taatactttt
     1321 attgttgttt gaacgagcag tacctaactc actaactaat tgagcataaa tattataagc
     1381 ctgagaagtt ggatcattat aggtacggtt catatcctta atattgcttt gttgactatc
     1441 aatctcgcta ttcttagaag taatatttgt ttttaatgct tcgatttttg ttgttatttc
     1501 tgatatctta gtttctaact tagctttgtt atcagctgat gtcgtatctg atagagaagg
     1561 tgagtctcct tgctgttgaa gttgaagcaa ttcattttgt agagcttgca agtcggcatc
     1621 taactgattt ctttcagctg ttattttatt caacttcgta tattcatcct gaatctttga
     1681 atactgggta ccattattat aattttccga atttaactga gataggtagt attcaacttt
     1741 cccataatat tcctgttctt caccagaatt agataaatga ttgtatttct catttaatga
     1801 ttgttcaaat ttttgaaaga tagcttcttt tgcacgtaac ttctccaatt gtttatttaa
     1861 agagctcgct tgtaaacctt cttgcccact tgataacgaa ataatgacat ctccagcatt
     1921 taccatatct ccttctgact tatgtaaagt aactaccttc cctgaaccaa ttgctgatag
     1981 gaactctgta cctgttataa ctgaatttcc attcgctttt acaatatagt ttttgggtat
     2041 ataagctgcg ccaaccaatg caccgcttaa gataatagca gttgaaataa tgagaataaa
     2101 cgcaaaagct ggtggtctct tatcaaagaa aatacgagaa taacgtaatt ctgatttatt
     2161 atataatttc ataggcttac aattggtcta aaaatatcta ctaccatttt ttcaggagaa
     2221 gaattaacat aaactgtata gacaatccca tccgtttgaa tatcattttc atagacatat
     2281 agatccaatt tagaatacgc atactgtaga tactctggac tgtcttcaaa acgaacatat
     2341 aaacaatatg gaacagagat agaatcctgt acatcataaa tgttactgta ctgttgagca
     2401 ttatgagctt gaatataaaa ctcaaaatca gtcgttatta atccatcatc atgaatagta
     2461 gtaccacaac tttttacaat taatggacca aaaatttgtg cttttaacaa ctgcaaatgt
     2521 tgatgaaatt tattaatttc ctgatctaca tcttctactt ttgtatcatg taacttttta
     2581 cagataactg actttagtac cagtttttta ttatctttta cctctaactt agccataagt
     2641 aacctcctct gtatctaaca cagcctgtga ctgaatttgt tgattcactt gaacgctctg
     2701 caaaccaacc attctagcat actttccatt tttcgccatt agttcttcat gagtaccata
     2761 ttccataata gtcccatttt ctaaaaagca tatcttatca catcgaagaa ttgtagacag
     2821 cctgtgggct actacaattg ttgtcttatc cattatttta ttaaagatta aatcctgaat
     2881 aatctgttca ctaaatgaat ctaagttaga ggttgcctca tcaaatatat acaaatcagc
     2941 tttactcagt agtgctcttg caatagccaa tcgttgtttc tctccacccg agaaattttg
     3001 accgccttct tctacaaagg agccataacg attttgaaga cgttgaatcg tatcatgaat
     3061 accaacaata cgacaaactc tcaccatatc ctcatatgta acagaaggat taccaatttt
     3121 tagattatca ataattgtcc ctgtaaagag ttctatggtc tgaggaatgt aggaaatttt
     3181 cttacgtagt ttaccatagt caagatctga taagtcattt tctcctatac gaattttccc
     3241 ctcactcgga tttataaatc ttaataacaa acgaactagc gtactttttc ctgctccgct
     3301 ttctccaaca aaagctattt tttctccttg tttaattcgc aaattaaagt ccttgataac
     3361 aggagggcgt gaaccatagg caaatgttac atctttaaat tcaatgtcat cacataaggt
     3421 gaagtctcta atactataat ccaaggcaat gtcttcacga tctacttcca tcaattcttg
     3481 aagccgacta accgcaacct gtacctcttg aaaggttaac tgcaagccaa ctaaattctg
     3541 aataggttct gtaaaatatt gacttaatgt ctgaaaaacc aagaggtccc caatagtcat
     3601 ttttccatca atgataaata aggcgcccac tcccataaat aataacccac ccatggttga
     3661 ggtcattgaa gatattgtag attgaatatt ctgcaaaaca ccctctttat aaccaatttc
     3721 taaagtatga acaaattttt cttcaatttt gtttaaacgt tgttcctcat catgctgaga
     3781 cttaatggta tcaatgttac gaatagattc aattaactgt gaattaagaa gtccatttgc
     3841 ttccatttgt tcatgattaa tttttttata aggcttttta aaacagtata taagaatgat
     3901 gttaacaata accataaaaa ctaatatcag aaataaagat tgattaatag tccaaaggac
     3961 aaccgcacta ataactgaca aggtgatatc catgactaat gaaatagaga ctgacgtaaa
     4021 cacattcttt atcgtcatcg catcttgaaa tcttgttaaa acatcaccaa ctctacgact
     4081 tccaaaaaaa ctataaggaa gatgaataat atggtcataa tatcccatta aaaccggtat
     4141 atcaattttt cgagatagaa acaaaagaac gtgctgacga aatgcagata gaagagtctg
     4201 taagaacgac actattccaa atactatcaa aaacatatac agactatttt ttaaagcata
     4261 tggaatcacc tcgtccatga taacttttga aaagagactc gataaaatac caatgatact
     4321 taaaacaaag gaagctaata tgactgtact aatcaatcct ttttgattaa aaattaacct
     4381 tccaaataaa tcaagaagtc cttttccctt tagtttccct ctttcaaaat ccgaatttgg
     4441 aaccataaaa agagtaatac cttgataaat ttctcccagt tcgtcacttg acatcttgcg
     4501 tatgccacta gcagggtctg ctacgtagaa cttactattc cttttgatac tatgtagtac
     4561 cacaaaatga ttttgaccta aatcattctt aacttgaaga atcgcaggaa atgttaattt
     4621 tggagtcaaa tcttccagtg ctactcgaac agcttttact gtaaaattca acttattcag
     4681 acctgaaaca ataccactga cagtcgttcc atacatgtct gtaccaataa tttccctaat
     4741 cttcatgatt gatagttcct ttttgtaaga taaaagaact gttgaaacaa ctgccgcagc
     4801 acaatctgac tggtcatgtt gcatcgtaca aggatatttt tttaacatag aaatactcaa
     4861 ttctttagaa ttaatctaac ttttctttga tgtcacccaa acctcaatca ctgcccagta
     4921 gttcttgaat taatttattt tcaaccgtat cattctttgt tcttgatatc tttccaagca
     4981 ttgtagtaca gcagattttt gcccaaggac tgagcctcct ttaacaactc aaactccctt
     5041 acattatatc ggccagcccc atggaaagat tatagccaga ctaatcaatg atataataca
     5101 tactagaata caaaatattt ttttattcat actttttctc ctatctttat tttctaccta
     5161 catagtacct cttattaagc tcattttaaa attacgttaa cttttttcac aaatttattt
     5221 cgactttctt ttttactttt tacattgcta tgatacaata tttttaacaa aattttattg
     5281 tttgttaact tttttcacga aaaggagaaa atcatgcgtt atgatttcgg aaaggtctat
     5341 aaagaaatac gtgagtcaaa ggggctgacc caagaagagg tctgtggggg tgttctctca
     5401 agaaccagct tatcaaaaat cgaaagtggc aagacaaccc caaaatatga aaatatggaa
     5461 tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg aatatatctg tcaactttat
     5521 caaccaagtc aacgcacaga gattatgcaa acctatctca atatgcgttc cattatcggg
     5581 accagcgatc ttgtcaattt atttcaaaaa tgccaagact acctcaagac ccaccacgat
     5641 ctccctatag aagaaatcag agatatgctt gaagttgtta tttatctccg tcaacacggg
     5701 gcaggggaac tatcaaaaca cgcagaacag gttgtcaaaa aactttggaa aaaaattgaa
     5761 aaacaagata catggtatga gagtgatcta aaaatcctca ataccattct tttcagtttt
     5821 cccattgaat acctccatct catcactgga aaaatattgc aacgcctaga agtctataaa
     5881 aactatcaac atttatatga cttgcgaatg acaattctat taaacctttc cacactctat
     5941 ctatacaatc aagataaaaa catgtgtaag caaatctgct acactttact ggaggacgcc
     6001 aagaataaga aaagctacga taggctggct atctgctatg tccgtattgg gatttgtagg
     6061 gatgatgcga aacttatcca aaaagggttc tcccttctgg agctaaccga ggaaacttct
     6121 atactgtctc atctcaaaaa agaagtagag atctattacc aagcgaagga aagataaaaa
     6181 agccgaggga tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc
     6241 attgttaaac cagaagtttt tagctctgcc aagccacata gtgtgtttac tactatgtgt
     6301 ccatgtcacc atgaatcgcc gaacgattta acgagaaagt atgactttta cgtttatccc
     6361 aactcaatta tgacattttt ttcaaaagtc aatatatctc actttttcaa cgacaagaaa
     6421 gaggctgcca atcgaccaac ctcttattct gaacccatca ctccatcact ttttagcttc
     6481 attcgctttc ttagcgactg caatctggta ttcgacttgg tcaaaccctg tgcctcccaa
     6541 ggaatttcgt ctttgaacag cagttttagg ttgcaagtag acatagatat cctcggtaat
     6601 gagagaatga taggtttgta attcctccaa agtccaatct tgaagatttt tagcagactt
     6661 gatagagtct agcactaatc ttcccacaac ctcatgagct tctctaaatg gcaagccttt
     6721 ccctgccaaa taatctgcca actcagttgc atttgaaaag tccttctctg ttgattcttg
     6781 cattttttcc ttgtttacct gcaaactaga taacatacct gccaacacat cgagggaatt
     6841 taaaatcgtt tcgaccgtat caaacattcc ttccttatct tcttgcaaat ccttattata
     6901 agctaaaggc aaggacttca tgactgtcaa cagtccaaac aaatgcccat aaactctgcc
     6961 agtctttcct cgaatcaact cagccatatc tggatttttc ttttggggca taatagagga
     7021 acctgttgta aacgtatctg acaaggtaat gaattggtac tcaaaactac accaattgat
     7081 catttcttcg caaaaacgac tcatatgcat catgagtatg ctggcatttg atagaaattc
     7141 taagataaaa tcgcggtcac taacagcatc caaagaattt gtataaggtt gcttaaactc
     7201 cagcaaatcg ctagacaatt ggcgatcaat ggggaaagtc gttcctgcca aagctgccgc
     7261 acctaaaggg cataggtctg tatgcttctg gttaaattca aaacgttcac tgtctctttg
     7321 aaacatattg tagtaagcca tcaggtggta ggcaaaacta ataggctggg catgttgcag
     7381 atgggtatag cccggcatga tcgtcgccac atgattttcc gccaagtcta ataaaacacc
     7441 cttaagatga gccagtttat ctaggacata gcctagttgt tccttgagat acaagtgcat
     7501 atctgtcgca acttggtcat ttcgagaacg agccgtatgt aacttccctg caaggggacc
     7561 aattttttct gtcagcaaca cttccatatt catatgaata tcttcgtttg cgatatcaaa
     7621 gtcaagttga cctgcctcta gctcttctaa aagctctttc aagcctactt gaatcttttc
     7681 tgactcctcc aaactcaaaa tgcccgtctg acccaacatc tgaacgtggg ctaaagaacc
     7741 aatcacatca aatttagcta atttctggtc aaaagatata ctcgcaccaa agcgctctac
     7801 ccagtcttcg acagtacctt caaagcgacc accccataat tttgtattct tagccatatc
     7861 acatccacct ctctatccaa tcgtcctatt tggcgctttt ttgaacctca gaatgaacct
     7921 tggttggaag tccccataac tcaataaagc caacagccgc atcttggtca aaggtatctg
     7981 cactagtata cgtcgccaaa ttttcatcgt aaagagaatt tggtgatttc cgagccacga
     8041 cctgagcgct cccccttata cagtttgact tttgcagtcc cattgacaac tttttgagtc
     8101 tctttaatat aagcaatcaa ggcttgtgta gccgaactaa accacaaagc attataaatg
     8161 agatttgata attcattttc tatgattggt ttaaaatgag ccacttctct cacaagcgtc
     8221 aagtcctcaa tttccttatg agctgtcagc agggtcactg cacctggaca ctcatagatt
     8281 tctcttgatt taatcccaac caagcgattt tccacgtggt caatccgtcc aaccccatgt
     8341 tttccagcta tttcattgag tttttgaatc aaatctgcca attttaacac ttctccattg
     8401 agggaaactg gaacaccttc actaaactca atctcaatgt attctggcat atctggagct
     8461 tgctctggag aagttgtgat gccaaaggct tcttctggag cctgattcca tggattttcc
     8521 aaaataccac actcattggc acgtccccaa agattttggt cgacagagta gggattgtca
     8581 aggtcagcag gaacaggcac cccatttttc ttggcataat aaatttcttc ctcccgagac
     8641 catttccatt cacgaactgg agcaatcact tttaaattgg gatccaaggc tgcaatcgat
     8701 acttcaaaac gtacttggtc attcccctta ccggtacaac catgagcaat tgtagtcgct
     8761 cctatctgat gcgctatttc aaccaatttt ttagaaatca gagggcggct caaggcagat
     8821 accaagggat acttttgttc ataataggca tgtgactgat gagccactag cacataatct
     8881 gtagcaaatt cgtccttaac atcaatgaca taagattcta ctgccccaac cttaagagcc
     8941 ttatcatgga tgaaatctaa gtctttccct tcacccacat ccatacaaac tgaaacaaca
     9001 tcatagtctt tctttaacca ggtaatagca actgatgtgt ccaaaccgcc agaataggcc
     9061 aaaattactt tttctttact catgttcttt tccttctttc tatttttcaa tggatgcttt
     9121 aaaggcatcc tcaatgagtt tgtctaattc cccagactcc ttcaactttt gaatagtttt
     9181 atcgactgcc tctttcaatt ccttactatc ttttttcatg gctaccgagt aggaatcatc
     9241 ttgctctttt tcaaaattga ggtctgcgat tgctaaatca ggattatttt ccacaaagcc
     9301 cttggcaact ggttcttcaa agataacggc atccacttgt cctgatttta aatctgtgat
     9361 taaattccca tttttaggca gagatacgag ggaagaattt tgtagcaaat ctttcgccat
     9421 cgtctcttga atcgaacctt tctgggctcc gaccttcttc tgcgctaagt cgttgacaga
     9481 ctgataagtg actaaatcag attttttgac aatgagttta ttttttgcag tatagtaggg
     9541 aatggaaaag tcaaacacct tgctccgttc atctgtctta gaaacacctg atatggcaag
     9601 atcagctttt cctgattgaa cactagccag tacattgtca aaactcatgg gagatagttc
     9661 caattcgaca cctagttctg ttgcgatagc cttggctaat tcgatatctg aacccacaat
     9721 ctgatttttc ccatcaacca ctttttggta ttcaaatgga gcaaaatctg gattgagggc
     9781 tacaaccaat tttcctttgg acttgatggc ttcaattcca gccgattgat tgttactaca
     9841 agcaaatagt agggggagca tcactaaacc aaacatcgtc atcaacacct tcttcatctt
     9901 attcataaca aagcctcctt tatgtttatt ccttttgttt gtataaataa tacacctatc
     9961 ttcatccttt gtcaagccta aattgtaatt tttttcaatt taaaacagaa aaacctagga
    10021 aaattacata aaaatgtcat tcctaggcgt atttatgcta ttcctctctg gaaaatatga
    10081 gtattcagtc agtcaaaaga agctgaacga actcattttc cctcgcccaa ttcaataatc
    10141 cgatgacatt gttgggctac ataagcatcg tgggtcacaa taatgactgt tttcccttct
    10201 cgattcatct ctaagagaaa cttcaagacc aaatctctat tttcaggatc cagcgaacct
    10261 gtaggttcat cggctaaaat cagctggctg ggttttaaga tggctctagc aactgcaatt
    10321 cgttgttgtt cgccaccaga caactcggag accttttgat gcaaagtagc tgacaaacct
    10381 actctctcta aaatctcttc cacctttttg agcttatctt tcttagacaa ttttacatat
    10441 ttcagcgcca gcataagatt gtactcgacc gtttcatcat caatcagggc aaaattttga
    10501 aacagataag agatatgttc acggattatt gtttgcgact tagcagaatt gaccgctaga
    10561 tttgtctgac caaaaatctc ataccgtccg ctataatcac catctatcaa acccaataaa
    10621 tttaacaagg tcgacttccc actaccactc ttaccaacaa tggcaaccaa atccccctga
    10681 tcaatcctga gagacaagcg atccaaaatc actttccccc caatggtttt ggtaatattt
    10741 tccaactcaa tcataagatg ccccctttca ataactctac tagacttctt ttctccatcc
    10801 tagaagccaa ggctagcaca aatagtatat ccaaacatgt aaagcctgca aacagcagga
    10861 gtggcaagaa cgcatgggca aagaaaatca agactagaag agggagacta tagcccagca
    10921 agagcagaac gaggagagga cggtagcgat cgaccagttt ccaccccata aacttcttgg
    10981 taataatatc cctgcgcttc aataagaaag ttattactag taagaagtag gaaatcatca
    11041 tgctaaagag accaaacaaa gcaaagagta ggttaaaatt ccgaacagca tctcgataag
    11101 aatccacttt ctcttgttga atggcttgaa tagatgaaaa ttttaaataa tttccatctg
    11161 acaatttctc aactaactct gtaatctctt tttgatgttg aaccgtattt tcaattttaa
    11221 tcggattatt taagccagtt gttgacaggg aggctttctc atcccacatc atatcagaat
    11281 cattgaccaa gctaataatt ggattggaga gattttcctt tcgcttatca ctatatggga
    11341 aaaatgacca atctccttca taataggcaa tctcgacatc catctcctct atcgttcgtt
    11401 tttgctgctc ttcatacttc atcgaatgaa aggcaattaa cttccccaag agctgatttt
    11461 tatcttcttc acctttcgta cttgctggca tcaaaataac ttttttaata ccagtatctg
    11521 gtagcttgaa tcccttgctc tttagaaaat tgcgattggc atagtaaaca tccaccgtat
    11581 ctgttaactg atattgctga atctgttctg attggacaaa attttttaca ggaagactgc
    11641 tactctgcac atagcccgcc tgcgtttttt ctactaaatc ctgataaaat cgatagaaat
    11701 aatccaatgc cttccctgag cctgccaact gctcttgcca caagttgtca ttgagttgga
    11761 aagtttctaa ggtcagataa tttccttggc tgatccactg tttctggtaa tccaactctt
    11821 tgttttcttg ctccaaactt tttcctaccc caactagcaa gactgtcagt aaaatggtag
    11881 ttccaatttt catcaaataa ttaaagagta gaccaaattt gaaagatgaa aaacctttca
    11941 gcagagagct gattgtcatt ttttggatta agaggtaagt caaccaactg ataaagagat
    12001 aaagctgcaa cagcaaaaaa tgagacaacc acagcatagg aaacaaatct tttggcttat
    12061 aatcaagcaa gaaaaacacg cctagattga tcacaagagc cccacctagg aggaggtaaa
    12121 ggttgccttt tacaacatca gctaaaacag ccctatcttg aaaaccaagt aatttttgta
    12181 ccccaactcg tttcatctcc atcatcggtt gatacactgt cactaacaca agaagcaaaa
    12241 tagccaagac aaaaacaatg gcagataaaa gcaaatctcg atttatgact tccactgcac
    12301 ttttgtaggt cggctctagc aaggtagcct ggtctatctt gaaaaaatcg ctccatttct
    12361 gtacaatcct atccttgtcc atctcttgtg tagaagttat cgtatagcga ccatttaaac
    12421 tacgagatgt atccttgata taggtttgaa aagtcataag ctgaataggt ttggctttta
    12481 gaaaggtcgg aatcgtacca agtttattgg aaatttcttt attactatag actccttcac
    12541 catctgtggt aaaatcaaga gaagaaatcc caaactcttg gtaggggaag gtatctttat
    12601 caaaaacacc agacttgacc acctcatcgc cattatctac tttgataata gagaccttgt
    12661 cttggcttgc aacctcttca aaaaactgga gaatcgtttc tgtcggttta gtgacatctt
    12721 tcaagtacaa gtcaaatgaa taagttgtct tgcccatctc ctgaatccgc actatttctc
    12781 gtgtaacatt tacattcagg acaaaaaacg tagtacacag caagagcagg agcatacaaa
    12841 gatgactgat ttttttcata gagatacctc ccgtgaagaa actccctctc caaaagggga
    12901 gggagaattt taattaaaat ccatagaaat attccattcc cgttggtggg aatttgaaga
    12961 ttgaagcccg agcccacgcc tcagcttctg cacgatcctt agagaattta tttccatctt
    13021 taacagttgc tgtatgagag cgagtagaat gtaaataatc agaataacca aaatttcctg
    13081 accatcctac tccatagttc cattgaccac catcaaccca tactgccaaa acacttgtag
    13141 aagcaagtat actacatacc attaaagatg caacactaat tttctcatga tttatttctc
    13201 ctattatcga gagctattcc gtagaaacag ctatagtaat ttatcaacaa gtatcgtagt
    13261 ccattggact caccttcctt ctatctcatt tcatttttaa acaacgaact tactcttaac
    13321 taggagaata gttctcttct gtatcaggtc tttgaacaac cagagtagta ttaatcacac
    13381 tctaactatt actcaagaat cagcccaata aacaatatgg atagcgcttt caaattttta
    13441 aactctatcc catcctttta tctatattat gagcgaaaat ataataactg tcaagtaatc
    13501 gaagtgaatt ttataaaaaa aattacaagc caaatttgca aagtttacaa taagccgcta
    13561 gggcaatcgt ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg
    13621 cttagaagtc tataaaaact atcaacattt atatgacttg cgaatagcaa tcctgctcaa
    13681 cctttccaca ctctatctat acaatcaaga taaaaacatg tgtaagcaaa tctgctatac
    13741 tttactggag gacgccaaga ataagaaaag ctacgatagg ctggctatct gctatgtccg
    13801 tattgggatt tgtacagacg attctaaact tatccaaaaa gggttctccc ttctggagct
    13861 aaccgaggaa acttctatgc tgtctcatct caaaaaagaa gtagagatct attaccaagc
    13921 gaaggaaaga taaaaaagcc gagggatttc ctcgactctt tacatattat ttgttcgtta
    13981 gttcttaata ccagccgttg ttaagccaga agtttttagc agctgtccat gaaccgtaac
    14041 gtcctgcaac gtaggcatct gctacacgtt cttggttttc agctgagtag tcaccgttca
    14101 ggtatgaatc tgttaattgg taacgtccga tataacgtcc atttgtagct gtatagctac
    14161 cacctgattc tttttgagca atccattctt tggcttctgc ttcagatccg cttacagttg
    14221 aaacaactgt ttcacttact actggagttt ctgctactgg agctgaaaca gtttcatctt
    14281 gagcggctgg tgccgcataa gtagctggcg ctggtgttgc aacaggcgct acagggccat
    14341 cgataaccaa ctcttgacca acataaatca aatgaatgtt atcaatgtgg ttgttttctg
    14401 ccaatttttc aactgttgtg ttgtgagttt cagcgatttc tgaaagtgta tcaccttctt
    14461 taacagtgta agttgatgat tcttgagcag atacaaatga tggagcaaat actgcaaaca
    14521 aggcagctac tcctgcaaga gttgctttaa tctttttagt tattgatttc atattcgaaa
    14581 attctccttc tttctatagt tctatcatac cctt
//
LOCUS       DAVLFB010000044        14166 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLFB010000044 DAVLFB010000000
VERSION     DAVLFB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.044"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..1079
                     /locus_tag="VXD58_001199"
                     /pseudo
     CDS             1..1079
                     /locus_tag="VXD58_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1164..2366)
                     /gene="rpsA"
                     /locus_tag="VXD58_001200"
     CDS             complement(1164..2366)
                     /gene="rpsA"
                     /locus_tag="VXD58_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV0675906.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2528..2599)
                     /locus_tag="VXD58_001201"
     tRNA            complement(2528..2599)
                     /locus_tag="VXD58_001201"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2565..2567),aa:Gln,seq:ttg)
     gene            complement(2609..2689)
                     /locus_tag="VXD58_001202"
     tRNA            complement(2609..2689)
                     /locus_tag="VXD58_001202"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2653..2655),aa:Tyr,seq:gta)
     gene            complement(2739..2969)
                     /locus_tag="VXD58_001203"
     CDS             complement(2739..2969)
                     /locus_tag="VXD58_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV0675907.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3137..3781)
                     /gene="pcp"
                     /locus_tag="VXD58_001204"
     CDS             complement(3137..3781)
                     /gene="pcp"
                     /locus_tag="VXD58_001204"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0675908.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3796..4719)
                     /locus_tag="VXD58_001205"
     CDS             complement(3796..4719)
                     /locus_tag="VXD58_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV0675909.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMSNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4716..5405)
                     /locus_tag="VXD58_001206"
     CDS             complement(4716..5405)
                     /locus_tag="VXD58_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV0675910.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5470..6174)
                     /locus_tag="VXD58_001207"
     CDS             complement(5470..6174)
                     /locus_tag="VXD58_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675911.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(6230..7252)
                     /locus_tag="VXD58_001208"
     CDS             complement(6230..7252)
                     /locus_tag="VXD58_001208"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV0675912.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7384..9855)
                     /gene="parC"
                     /locus_tag="VXD58_001209"
     CDS             complement(7384..9855)
                     /gene="parC"
                     /locus_tag="VXD58_001209"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV0675913.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10275..12218)
                     /gene="parE"
                     /locus_tag="VXD58_001210"
     CDS             complement(10275..12218)
                     /gene="parE"
                     /locus_tag="VXD58_001210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV0675914.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12354..12995
                     /gene="plsY"
                     /locus_tag="VXD58_001211"
     CDS             12354..12995
                     /gene="plsY"
                     /locus_tag="VXD58_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV0675915.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13038..14166)
                     /locus_tag="VXD58_001212"
                     /pseudo
     CDS             complement(13038..14166)
                     /locus_tag="VXD58_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4074 a         3177 c         2482 g         4433 t
ORIGIN      
        1 tagaaagcgt cgattcaagt gctatcactg ttcaaaaatg atggtcgctg aaacttctat
       61 cgtcaagaag aatcaccaaa tccctcgtat catcaaccaa aagattgctc aaaagttaat
      121 tgaaaagatt tctatgaccg atattgccca tcagctttcc atctcaactt caactgtcat
      181 tcgaaagctc aatgacttcc actttgagtg taattttaga aatctgcctg agattatgtc
      241 ttgggacgtt gaaacagtcc ggggagtgac tgtttcaatc gggagatgga gatgagcttt
      301 attgcgcaag attttgaaaa gctcaatatc atcactgttc ttgaaggtag aacacaagct
      361 atcatccgag atcactttct tcgctacgat agagccgtcc gatgtcgcgt caaaatcatt
      421 actatggata tgtttagtcc ttactatgac ttagctagac aacttcgctt tcaaatttct
      481 aggctcaggc tgaaacagtc tcccagactg tttcactccc gaatgctaaa atcgttcttg
      541 atcgctttca tattgtacaa catcttagcc gtgctatgag tcgtgtgcat gtccaaatca
      601 tgaatcagtt tcatcgaaag tctcatgaat acaaggctat caagcgctac tggaaactca
      661 ttcaacagga tagccgtaaa ctgagtgata agcgatttta tcgttctact tttcgtatgt
      721 acttaaccaa taaagagatt ttaaacaagc ttttgagcta ttcagaagac ttgaaacacc
      781 actatcagct ctataataag gaaccagaga aatttttcgg acttattgag gacaatctta
      841 agcaggttca tcctcttttt cagactgtct ttaaaacctt tctaaagaac aaagagaaaa
      901 tcgtcaacgc ccttcaacta ccctattcaa acgccaaatt ggaagcgacc aataatctca
      961 tcaaacttat caaacgcaat gcctttggtt ttcggaactt tgaaaacttc aaaaaacgga
     1021 tttttatcgc tctgaacatc aaaaaagaaa ggacgaaatt tgtcctttct caaccttagc
     1081 tgacttcaac ccactacagt tgacaaagag ccagaaaaat cccttgttta gtaaacaaag
     1141 gattttgtga attttcaatt tgattaaagt tcgatatcac caaacaaatc agccattgaa
     1201 aatcctgttt gtgtttctgg aagttcgaaa tcacgctttt cttgacgtct tggacgacgt
     1261 ggacgagcag cacgtttttc ttctttttgt ccttcttctt gggctggacg ttcttcaaga
     1321 gctttaatag aaagtgatac gcgttctgca tctgcgttaa cttcaagaac tttaacttga
     1381 acttcttgac caactttaag agcttctttt ggattttcaa tccgtttgtg tgaaatttgt
     1441 gatacgtgaa caagtccatc gatacctggc aatacttcaa caaatgcacc gaagtcagtc
     1501 aaacgtttaa ctgttccttc tactacatca cctttagcca atttttgctc aacgccatcc
     1561 catggtcctg gtactgttgc tttaagtgaa agtgatacac gtccttcttc ttcgttaaga
     1621 tcaaggattt tcacttcaat ttcttcacca acagttacaa ctgattttgg tgatacgtta
     1681 cgttcatgtg acaattcagt caagtgaacc aatccgtcaa caccaccaag gtcgatgaaa
     1741 gcgccgaagc ttgtgatacg agcaacttta ccagttacaa catcaccaac agccaattta
     1801 ccgaatactt cagcgcgagc tgctgcagta gctgcttcaa caacttcacg acgtgaaagg
     1861 atgaagcggt tttctttagc gttaacttct ttgattttag tatcaaattc ttgacctaca
     1921 aaacgctcag cgttacgtac gaaacgagta tccaacattg aagctgggat aaatccacga
     1981 acaccttcaa attctactga aagtccacct ttaacggcac gcgttccttt aacagtaaca
     2041 acttcttctt cgcgaccaac aagtttgtcc catgctttgc gagcttcaag gcgtttttta
     2101 gatacaaggt atgtaactgt atcagtatct ttaccaacta cttgacgaag tacaagaaca
     2161 tccaatactt ctcctacttt aacaaagtca ttgatatctg catcacgatc gtttgtcaat
     2221 tcgcgaagag tcaagacacc ttcaacacca gttccagaga ttgcaacgtt agcttgagtc
     2281 gcatcaactg tcaatacttc agcactaaca acatcaccag tctcaacttg actaacgcta
     2341 tttagcaaat cttcaaattc gttcatctaa aaaatcctcc aacaatcaag tttttcttaa
     2401 acttgacata cttataattt ttttcctaag cacccgcaag gacatgttca tatcattcac
     2461 gtctttcaat tataaaaata aaatacccta agatattttg ctttttatct tgattattac
     2521 ctaagaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
     2581 accgcttggc tataccccat taatgaaatg gagagagagg gattcgaacc cccgaacccg
     2641 aaggagcgga tttacagtcc gccgcgttta gcctcttcgc tatctctcct acaatcaaca
     2701 tggactatta tatcatgaaa atttcaaaaa aacaagactt attttgctaa attataattt
     2761 tcaatcaaaa tttccacagc tttttccaat tttttataaa aactatcgac attcccattc
     2821 tttataatgg caaattgtcc gtctaattcg gcaatttctc cgataacttt tttaccgata
     2881 gtcaatgtat aaccttcaaa actgtcttta ccaacattaa ctttggcatc cgctacttga
     2941 atttcgattt ttttatcttt cttgctcatt tcatacctct cttcactttt tctattttac
     3001 aagaaaatcc taaaactagc aagaaaaaac tctatatcag gcttctctga tatagagttt
     3061 tatgtaaaca acctcaatcg atttagcata gctaatagtt agtaaacaag cataattgac
     3121 tatccttttt aggttatcag tgagtctcac cgcctactaa ctgcaaatcc ttatctttgt
     3181 agtctacaat agcttcaata gctacctcta tgcctcttac aatatcacat agactcattc
     3241 ctgcagtatt cggcttattt actacttgtt ccatcatata tggaatatgc atgaaacctg
     3301 ctctcatatt agggaatttt ttatccacta aataaagagc ttggtacatc aaatgattgc
     3361 aaacaaaggt tcctgcacta ttggatacaa ctgccggaag tccctgtttt ttgatagctt
     3421 gtaccatcgc tttgataggt aaactactaa aataggccga tgctccatca atacgaatcg
     3481 gtgtatcaat tggttgattg ccttcgttat caggtatgcg agcatcatct tgattaatag
     3541 ccactcgttc aggtgttaag ccggtcctgc cgcctgcttg tccaatacaa agtacagcat
     3601 ctggttgata tcgtaatatt tctgcctcta aaacttctga cgacttataa aaaaccgttg
     3661 gaatttctac ccagcgaact tcagccccat taatctcaga tggtaataat tttacagcct
     3721 ccaaagctgg attaatcttt tcacctccaa aaggattaaa acctgtaacc aatattttca
     3781 ttttattttc ctttactaaa atgcgagaaa gtacattaag aatatgtgaa taacaatcat
     3841 tactagagca acacctgctt gagcctttat aacgccattc tgatctttca tatccatcaa
     3901 tgctgctggt agagcgttaa aattagcagc cattggggtc aataaggtcc cacaataacc
     3961 tgctgtcatg gcaagagcac cagccacaat tggattagct cccagagcaa atacaaaggg
     4021 aactccaaca cctgctgtaa taacggtgaa tgctgcaaaa gcatttccca taatcattgt
     4081 gaatagaacc attccaagaa cataggccaa aactcctata aagcgactat ctgaaggaac
     4141 aataccgcta atcagatgag agataacatc accaacacct gctacagtaa aaatagcccc
     4201 caaagcccct aataattgag gaacaatacc acttgttgaa acttgctgag tcattcgatt
     4261 attttctgat aacagactct tagggtgact attggtaatc acaagaacag aaattgtagc
     4321 aaacaaggcg gcaaggctaa tcgaaatctt gctaaattct ggaatcattt gcgctaagac
     4381 caacgcaagt attgccatca gcataactgg aataaaaatt ttatttttca acctgttaga
     4441 ttcaatattg gctttcattt catctaagga tggcaaggtt ccgatacgga cttgcttaaa
     4501 caatgttaac agcgataata ggattacaat aataccaata ctcatatttg acatatagga
     4561 accacctata aacgtaatag acaatagagt ccaaaatgca gatgtcccaa gtcgaactgg
     4621 gtttgtttta tctttataac tacaataggc tgtatggaga aattgacaac caatcacaat
     4681 ataggtcaac tctaatagtt gctttgccaa ctctgtcatt tttgttctcc tcccctagtc
     4741 ttttttgata tcaatttttt atcaaataaa taattgtaaa tccccactac aataagtgtt
     4801 ataacagcaa caataataga tgtagaagca atccctgcat aattgctttc atagcctaac
     4861 tgatctaatg ttccccctat caagaggact cccccagcac ctacaaacgt attttgagca
     4921 aagaaatttc caaaattttc attcgcagcc gcacgcgctt ttattgtctc atcttcaacc
     4981 tctgttaact ttctacctaa ttgagactct gcagctgctt ctcccatagg ttgaaccaaa
     5041 ggtctgacaa actgagggtg tcctcctaga cgaattgaaa agaaaccagc taactctcga
     5101 ataaaaaaat aaactgtata gaagtttcca actgtcagac ctttaatctt tcgaatcaaa
     5161 tcgattgatc gttgcttgag tccaaaggtt tctgacagcc ccacaagagg caaggtaacc
     5221 ataaaaatcg tgagcactcg ctgattgcta aattcttttc ccaaaatctc caaaaattca
     5281 acgagagaaa cacctgaaac taaagctgta accaaaccag ctaagactac tgttgcaatt
     5341 gtatcaaatt ttaaaataaa acccacaaca atgattgcta ttcctattaa tctaatccac
     5401 tccatatcaa actcctttat attcaaaatg acagtatttt aaaaatttta tcaagatcaa
     5461 taccattcct tatttaatgt atttttctag ttctttttgg tatttgctat tggattccaa
     5521 tttttctttt tgccattttt taaaaacctc gttatattct tttgttgtaa caatatcttt
     5581 ttgcaatttc attcctttaa agatatatgg ttccccctta ataccaactt gtgagtatgg
     5641 ttttgagaat ggtactacgt tacttacaac tggagaacca ccagatgaag ctgttggcat
     5701 caataatgaa ctatctgtcg accaagcttg agctttggca tatttttcat atctcttctc
     5761 taggtcagtg gtctcagaaa cagcatcttc taacaatttc ttatatttat ccaaaccagg
     5821 tttagctaca acatccttat cttttccttt cgtaatacca aggtgtttca tggcagaacc
     5881 agattttgga tccataatat tcaagtaaga cgctggatct tgatagcttg gagcccatcc
     5941 tgtactattc aaatcatagt ctttttgaga aggagcaaca ttgccgtatt tatcattttc
     6001 catcaaacca tcaataacat ttccaataac gtctgtcctc gatgttcgag tcgctatact
     6061 gtagcccaat gatgctggat ctactgcata gacataagaa aatgttgtcg gtgcatctgc
     6121 ttctttatca gtttttccac aagccactaa aatagctgac gtgctcagga ccactcctgc
     6181 tgttaagagc cactttttct atttcataaa aaatctcctt tggtttattt taatctactt
     6241 ttacaatcca accttctggc gcttcaatat cgccaaactg aatacccgtc aattcattat
     6301 ataatttacg cgtcacagga cctacttctg tttcactata gaatacatgg aaatcatcac
     6361 catgttgaat acctccaatt ggagaaataa ccgctgctgt accacaggca cctgcctcta
     6421 caaaacggtc aagattatca attggaacat caccctcaat aggagttaat cccaagcgat
     6481 gttctgccaa ataaagcaag gaatacttgg taatagatgg caagatagat ggactcaatg
     6541 gtgttacaaa ttcattatca gctgtaattc caaagaagtt agctgatccg acttcttcaa
     6601 tctttgtatg agttgatggg tccagataga taacatctga gaaatgacgt gacttggcca
     6661 tttttcctgg taagagactt gcagcatagt ttccaccaac cttagccgca cctgtaccat
     6721 ttggtgctgc acggtcgtac tcatcctgaa tcaagaagtt ggttgggacc aaaccacctt
     6781 taaagtaatt tccaactggc atagcaaaga tggtgaaaat gtactcttct gccggtttta
     6841 ccccgataat atctccgaca ccaatcaaaa gagggcgaag atataaggtt ccacctgttc
     6901 cgtatggtgg tacgtattct tcattcgcac ggacaactgc tttacaagct tctacaaaca
     6961 tgtctgtcgg aacttgtggc atcaagagac ggtcacatgt acgttgcaga cgtttagcat
     7021 tttcatcagg acggaacagt tgaacactgc catccttagt acgataagct ttcaaacctt
     7081 caaatgcttg ttgtccatag tgaagacttg gagaagactc tgaaatatgc aaagttgcat
     7141 cctctgtaag ctctccttga tcccattgtc catttttgaa atgagcaaga tagcgataag
     7201 gtaatttcat ataggaaaaa ccgaggtttt cccaatcaat cgttactgtc atattccact
     7261 ccttattcta aaaacctatt tcttatattc tacactattt ttctaaaata gcaagtatat
     7321 tttgtaattt tcagaaaatt tctccaataa aaaccagctc ttagaactga ttcttcattt
     7381 cacttattta tcttcagtaa ctacttcctg aagataagcg tcaaaaactt cttcatctga
     7441 aatcgtgtca gaaatgaagc ttccattgct agtgcgttct gacaagttca agtcttgcaa
     7501 tcggctttca tagattgttc ctttattgga ttggacaagc agagtttggt cgttcacatc
     7561 cacttccgta ctgaagaaat cgccaacaaa tccttgctct gcaactgctc ctgccaagaa
     7621 gacacgatgc ggtttgtttt tcaactcacg caagacttgt aatcctcgtt tggcacggct
     7681 ggttgctaga atttcctcaa tggaaacacg tttcaagctt ccacgctggg tcaagaggta
     7741 gaaggacgaa gtattacaga taaagccaga ttggaggaca tcatcttctt tcaaattcat
     7801 agccttgaca cctgctgcct tagcaccgac aaccggaacc tcttcgatat tgaaacgcag
     7861 ggcataacca ttttgactaa ccaagacaac atcatctagt ttaatcggag ccactgctac
     7921 aatctgatct gtatcgtctt tgagcttagc atacttgaca gacttagatc tataggtccg
     7981 ccatggagtg aattcttttc gctctacccg tttgatttga ccaaggcgag tcactgcaaa
     8041 gtaggttgtc gcatcgtcaa actgatccag tacttccaca taaaggattt cttcattcgt
     8101 ttcaaagttt gtgatggttt ggctcagatg ctctccgatg tccttccaac gaatatctgc
     8161 caactcatgg attggtctgt agatgacatt tccaagactt gtgaacatca agaggtgctg
     8221 ggttgtcttg gcagattgaa caaaaatcaa acggtcatca tcacgcttgc caatttcttc
     8281 caaggtggaa gccgcaaagg aacgtggact ggtacgcttg atgtaacctg ccttggtcac
     8341 gctgacgtag gtatcttcct cagcgataag actagctgta tcaatctcaa ttgctttcgc
     8401 agtgtcttct aaagaactca aacgaggagt tgcaaatttc ttcttgacct cacgaagttc
     8461 tttcttcatg agattgtaca tagtcctttc atcaccgata atagccgcca gcatagcaat
     8521 cttctcacga agctctgctt cttcttcctg caagacaacc acatcggtat tggtcaaacg
     8581 gtacagttgc aaagttacga tagcctcagc ctgttcttcc gtaaaatcat agctaacttt
     8641 gaggttttcc ttggcgtccg ccttattctc agaagcacgg ataagagcaa tgacttcatc
     8701 caaaatcgaa atcacacgaa tcaaaccttc gacgatatgg agacgtttct cagccttttc
     8761 tttgtcaaag cgtgaacgcg ccaaaatcac ttctcgacgg tgagcgatgt agctagacag
     8821 gattggaaca atcccaacct gacgaggtgt gaaattgtca atcgccacca tattaaagtt
     8881 gtagttgatt tgtaggtcgg tgtacttaaa taagtagttg agaacaagct cagtattagc
     8941 gtctttctta agttcgatag cgatacgaag accatcacgg tcagactcat cacgaacctc
     9001 agcaatccca gctaccttgt tattaacacg aacatcatcg attttcttga ctagattggc
     9061 cttattgatt tcataaggaa tctcagtaat aacgatttgt tccttaccac cttttagctt
     9121 ttcaatttca gtcttggaac gaacaaccac gcgccctttc ccagtttcat aagctttctt
     9181 gatttcatca cgaccctgaa taatagcccc tgtagggaag tctggtccag gcaagaattc
     9241 catgagttta tcaatctttg cagttgggtg gtcaatcatg taaactgcag catctatgac
     9301 ctcagctaaa ttatggggag gaatgtctgt ggcataacca gccgaaatcc cagtcgaacc
     9361 attgaccaag aggtttggaa aggctgctgg caagaccgtt ggttctttct ccgtatcgtc
     9421 aaagttccat gcaaaaggaa ctgtattttt ctcgatatcc tgaagaaggt aacctgcaat
     9481 ttcagacaaa cgtgcctcag tataacgcat agccgcagga ggatctccgt ccatagaacc
     9541 gttattaccg tgcatttcaa ctagaatctc acgatttttc cagttctgtg acatacgaac
     9601 catggcatca tagatagaag aatccccgtg tgggtggaaa ttccccatga tgttcccgac
     9661 tgacttggcc gacttacggt agctcttgtc aaaagtattg ctatccttat tcatagaata
     9721 aagaatacgg cgctgaaccg gcttcaaccc atcacgaata tctggcaaag cccggtcttg
     9781 aataatgtac ttggagtagc gaccaaagcg ctctcccatg atgtcctcca gggacatgtt
     9841 ttgaatgtta gacataagat acaaagccca taaaatacca agtgaaaata gaaaattctt
     9901 gaagtaagca aactcacaag agaatttatc tttttcacac agtatctagg gcgtgttcaa
     9961 ctcctttcaa agaatgtaga gtagtttttt atgcagtaaa agatatttta cgggaattcc
    10021 tcccgtgttc agttacgata agtaaccaaa ctatcctgtt tgtatttttc aatatgaaaa
    10081 tctggttttc caaaattagt cttagtttgt gtcttagccg ctcccttaag cgcctctttg
    10141 agataagcac tcatagcaga ttcttcatta ataatcctgc aattttttca aaccaagatt
    10201 ttcaaactgc tttttcacat agtcattcac atccgactct aatttccagt ttactaacat
    10261 attattttct ttcattaaaa cactgtcgct tcttctagcg taaacttgac attatcttca
    10321 atccatttac ggcgtggttc taccttatct cccatgagaa cattgacgcg gcgttcggcg
    10381 cgcgctaaat cttcaattgt gacacggatg agggtacgtg tttctgggtt catggttgtt
    10441 tcccagagct ggtccgcatt catctcacca agtcctttgt atcgttggag ggtagcgcct
    10501 ttaccgaact gtttacggag ttcttctagt tctccgtccg tccaagcgta ggccacttct
    10561 tctttcttgc ctttaccttt ggacatctta taaagaggtg ggagggcaat atagacatga
    10621 cctgcctcga ctagcggacg catgtaacgg tagaaaaatg tcaagagcaa ggtctggata
    10681 tgggcaccgt cggtatccgc atcggtcatg ataatgatct tatcatagtt ggcatcttca
    10741 atagagaagt ctgctccaac acccgcacca atggtataaa tcatggtatt aatctcttca
    10801 tttttgagga tatccgccat cttggccttg gctgtattga caaccttacc acgaagaggt
    10861 agaatagcct ggaacttgcg gtcacgacct tgtttggcag aaccaccggc agagtccccc
    10921 tcaactagat agagttcatt cttagcagga ttcttagatt gggctggggt caatttccca
    10981 gacaacaagc ccttatcttt cttgtttttc ttcccatttc ggctctcatc acgcgcctta
    11041 cgtgctgctt cacgagcatc acgggccttg atagccttgc ggatgaggtt agaagctaat
    11101 tccccatttt ccataaggaa aaaggtcaac ttatcagcca ctattccatc cacaactggg
    11161 cgagctaggg ggcttcctag tttatccttg gtctgtcctt caaactgcaa gtgttcttca
    11221 ggaactaaga tagaaagaac gaccgctagt ccctcacgat agtctgaacc ttcaaggttt
    11281 ttatcttttt ccttgagaag acctgtttta cgtgcatagt cattcatgac cttggtaatg
    11341 gcagacttga atcctgtctc gtgcgttcca ccgtccttgg tgcgaacgtt attgacaaag
    11401 gatagaatgt tatctgagaa tccgtcattg tactggaggg ctacttccac ttgaaaacca
    11461 ttgtcttccc cttcaaagta aagaactggc gtcaagattt ccttatcttc gttgagataa
    11521 gaaacaaaat cttgtactcc attctcatag tggaactcaa tcgcttcatc tgttcgcttg
    11581 tccgttaaag acaaggtcac atttttcaag agaaaggctg attcattaag gcgctctgaa
    11641 atggtattgt acttgaaatc tgtcgtagaa aagatagtcg cgtcaggcat aaaagtaact
    11701 ttggtgcctg ttttagactt gggtgctgta ccgattttct tcaaagtcgt gacaggtttt
    11761 ccaccatttt caaaacgttg cttgtaaact gcgccatcac gggtaatttc aacttctaac
    11821 cagctagaaa gggcgttaac aacggaagaa cccactccgt gaagtccacc tgatgtctta
    11881 tagccacctt gaccgaattt ccctccggca tgaagaatgg taaagataac ctcaacagtt
    11941 ggaattccca tagcgtgcat acctgtcggc atcccacgtc catggtcttg aaccgttaga
    12001 ctaccgtctt tattgatagt tacatcaata cgatcaccaa acccagacaa ggcttcatcg
    12061 actgcattat caacgatttc ccaaactagg tgatgaagac cagcgccatc ggtcgatcca
    12121 atatacatcc ctggacgttt tcggaccgca tccaaccctt ctagcacctg aatagcatca
    12181 tcattataat tgttaatatt gatttccttt tttgacacaa ggaacctcct attcgttcat
    12241 ctttactatt ctacaggttt tccaaggatt ttgcaaaatt tttctttctc cgatgtgaca
    12301 atttcagcag agattctctg cttttctttc ccaattcatg atataatagg agtatgatta
    12361 caatagtttt attaatccta gcctatctgc tgggttcgat tccatctggt ctctggattg
    12421 gacaagtatt ctttcaaatc aatctacgcg agcatggttc tggtaacact ggaacgacca
    12481 acaccttccg cattttaggt aagaaagctg gtatggcaac ctttgtgatt gactttttca
    12541 aaggaaccct agcaacgctg cttccgatta tttttcatct acaaggcgtt tctcctctca
    12601 tctttggact tttggctgtt atcggccata ccttccctat ctttgcagga tttaaaggtg
    12661 gtaaggctgt cgcaaccagt gctggagtga ttttcggatt tgcgcctatc ttctgtctct
    12721 accttgcgat tatcttcttt ggagctctct atcttggcag tatgatttca ctgtctagtg
    12781 tcacagcatc gatcgcggct gttatcgggg ttctgctctt tccacttttt ggttttatcc
    12841 tgagtaacta tgactctctc ttcatcgcta ttatcttagc acttgctagt ttgattatca
    12901 ttcgtcataa ggacaatata gctcgtatca aaaataaaac tgaaaatttg gtcccttggg
    12961 gattgaacct aacccatcaa gatcctaaaa aataaaacgc cagttctgta ctgaccccaa
    13021 aaagttagac aattaattta tccgaaggat ttagttctgt attgcacagg gctaagtcct
    13081 tttagtttta ccttaattcg tttattgttg tagtaatcaa tatagtctac aatggcttgt
    13141 tccaattgct taagcgactg aaacgacttc tcataaccgt aaaacatttc cgatttcaga
    13201 atcccaaaga aggactccat cataccattg tctgggctgt tacccttacg tgacatggat
    13261 gcttgaattc ccttaccctc aagaaactga tgataagaat cgtgttgata ttgccagcct
    13321 tggtcactat ggagaatcgt attctcgtag tgcttctctg tgaatgcctg ttccaacatt
    13381 gtttgtactt gttctaagtt gggtgaagtt gaaagattat aggcgataat ttcgctgtta
    13441 aaaccatcta aaactggtga taagtaaagc ttttgagtac ttactggaat ggcaaattca
    13501 gtcacatctg tgtagcactg ttccattgtt ttagagcctt caaattggcc ttgaatgaga
    13561 ttctctgctt tcttaccaac gtctccttta tgagaagaat attttcgttt ctgtcgcatt
    13621 ttagcttgta aattgagtac tttcatcaag ccttgaactc ttttatgatt taccagataa
    13681 ccacgatttc ttagttctaa ataaatccga cgataagcat aatttccctt gtgttcgata
    13741 aaaatggatt gaatttcagc tttaagctct tggtccttat ctggtttgtc tagctgtttc
    13801 aagtgatagt agtaggtcga acgagctagt ttaatggctt ttagaagaat atctaacgaa
    13861 aactcagtca ttaattcttg aacaatttct gtctttcttc tttctctttt tcctccttca
    13921 atcggagttc tcttaacttt tttaggatgg cattctccgc tctcaggtac tcattttctg
    13981 cttgaagatg ttctaattct gtcctctctt caggtctctt ttttggctta cgtcccattt
    14041 taggtactct ccctcttgtt ttctcaacaa tagtataccc gtttttttct gtattgtgct
    14101 agccagttaa gaagtatcgt acgacttggg agaccgtatt caagagaaac tctgtcttta
    14161 gtccag
//
LOCUS       DAVLFB010000045        14108 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLFB010000045 DAVLFB010000000
VERSION     DAVLFB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.045"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(227..1834)
                     /locus_tag="VXD58_001213"
     CDS             complement(227..1834)
                     /locus_tag="VXD58_001213"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV0675916.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDTEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2224..2811)
                     /locus_tag="VXD58_001214"
     CDS             complement(2224..2811)
                     /locus_tag="VXD58_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV0675917.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2939..3388)
                     /locus_tag="VXD58_001215"
     CDS             complement(2939..3388)
                     /locus_tag="VXD58_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0675918.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3393..3782)
                     /locus_tag="VXD58_001216"
     CDS             complement(3393..3782)
                     /locus_tag="VXD58_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV0675919.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3810..4436)
                     /locus_tag="VXD58_001217"
     CDS             complement(3810..4436)
                     /locus_tag="VXD58_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV0675920.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4450..5013)
                     /locus_tag="VXD58_001218"
     CDS             complement(4450..5013)
                     /locus_tag="VXD58_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV0675921.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5178..5412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5466..5969)
                     /locus_tag="VXD58_001219"
     CDS             complement(5466..5969)
                     /locus_tag="VXD58_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV0675922.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6162..6992)
                     /locus_tag="VXD58_001220"
     CDS             complement(6162..6992)
                     /locus_tag="VXD58_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0675923.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6977..8659)
                     /locus_tag="VXD58_001221"
     CDS             complement(6977..8659)
                     /locus_tag="VXD58_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675924.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8761..9018)
                     /locus_tag="VXD58_001222"
     CDS             complement(8761..9018)
                     /locus_tag="VXD58_001222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675925.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9025..9573)
                     /locus_tag="VXD58_001223"
     CDS             complement(9025..9573)
                     /locus_tag="VXD58_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV0675926.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9588..10436)
                     /locus_tag="VXD58_001224"
     CDS             complement(9588..10436)
                     /locus_tag="VXD58_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV0675927.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10624..11973
                     /gene="trkA"
                     /locus_tag="VXD58_001225"
     CDS             10624..11973
                     /gene="trkA"
                     /locus_tag="VXD58_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV0675928.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11977..13416
                     /locus_tag="VXD58_001226"
     CDS             11977..13416
                     /locus_tag="VXD58_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV0675929.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13541..13798)
                     /locus_tag="VXD58_001227"
     CDS             complement(13541..13798)
                     /locus_tag="VXD58_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV0675930.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4139 a         3212 c         2610 g         4147 t
ORIGIN      
        1 agttcctaaa actttgctag aaattaattt ggctttccga attaatttgt tcagatttta
       61 tttcatttca atatactgat aaatccattc actagcacag ggcaagaggg agtcgctatt
      121 gatttcaacg gtagcaggta catacagagg actcctacct cagatatact gcaatatcat
      181 cgtatgcaaa aaacctctga gatttttctc aaaggttctg attttgctaa ttgctgttct
      241 caaccgctgc agtaacaaag gcagtgtaga gttcttctgg tcggtttgga cggcttgaca
      301 gttcagggtg atactgacaa gctacaaaga atttattttc aggaatttcc acgatttcta
      361 ccaaacgatt gtctggagaa actcctgaaa agacaaaacc tgctgcctca aactgctcac
      421 ggaaggcatt attaaactca taacggtgac ggtgacggcg ttgcaccact tcttgattgt
      481 gataagcagc agcagcctta gagccacgtt tcaacttaga cggataaagt cccaaacgaa
      541 gggttccacc catatcctca atatcaatct gatcacgcat gatatcaatg atagggtatt
      601 ttgtttctgg tgcaagctct gcagaattgg caccttcaag acctaaaacg tgacgagcaa
      661 actcgataca tgtcaactgc attcccaagc agactcccaa cattggaaca tcattttcac
      721 gcgcatagcg gatggcttgg attttccctt ctgtaccacg ttgaccaaaa ccacctggta
      781 cgatgatccc gtccgcatca gacaagagtt ctgctacatt ctctgctgtc acatcattgg
      841 cattgaccca attgatttta acttctgtat cattgacata gccagagtgt ttcaaggctt
      901 cgaccactga gatataggca tcttgcaact ccacatactt accaacaagg gaaatcttaa
      961 cttgtttctt gaggttcatg accttgtcca ccatggctga ccattctgtc atagccgctg
     1021 ctggtgcgtc taatttcaaa tgatcacaaa caatttggtc catcccttgt gcctgcaagt
     1081 tcagtggaat ttggtaaagg tgttcaacat ccaacgattc gataacggct tctggtgcca
     1141 catcacagaa ctgggccagt ttatttttaa ttccttgacc agctggctct tctgtacgaa
     1201 taaccaacat atttggttgg attcccaagc cacgcaattc tttgacagag tgttgggttg
     1261 gtttggtttt catttcacca gcagccttga ggtaaggaag caaggttgta tggatataca
     1321 tgacattatc cgcacccaca tctgccttca tctgacgaag agcctctagg aatggcaagg
     1381 actcgatatc tcctactgtt ccaccaacct ctgtgataat gacatcagag tcggtcgtta
     1441 gagcggcacg cttgattttt tctttcaaag catctgtgat atgaggaatg acttgaacag
     1501 ttgccccaag gtattctcca cggcgttctt tacgaagaac ttcactgtaa attttcccag
     1561 ttgtcacgtt ggaatatttg ttgagattga tatcgataaa acgttcatag tgacccaagt
     1621 ccaaatctgt ctcagctccg tcatctgtca caaaaacttc cccgtgctgg taaggactca
     1681 tggttcccgg atcaatattg atataagggt caaacttttg aatggttact ttgagaccac
     1741 gatttttcaa gagacggcct agactcgctg ccacaatccc tttcccaata gacgatacca
     1801 caccaccagt tacaaaaata tatttcgtag acatagattc ctctttctaa aatgctcaag
     1861 gtcttgttaa ctacgagggg atatagaaaa caagacccta ctaataagaa taatctggga
     1921 cgactgcacc agattcacaa ctaatactct tcgaaaatct cttcaaaccg cgtcaacgtc
     1981 gccttgccgt agatatgtgt tcctgacttt gtcagtctta tctacaacct caaaacagtg
     2041 ttttgagcag cctacggcta gtttcctagc ttgctctttg attttcattg agtataaaat
     2101 acaagaagat aacttcttga aaaaacaaaa atagctccct aagaactagg gagccccgac
     2161 ctctaaaaga ggtgcccaaa caatatgata cctaaaaata ggataattgt caatagctaa
     2221 ctcttattcc tcgctggttt cagcgtcgtc atctgaaaac tcgtcgtcgt cttcgttgag
     2281 atccacatct tctcccaagt catctggagc gatttcgttg atttctgcat cataagcttc
     2341 cacttcattt ttttcatcat ctggattttc atcatcgtat gaaagagctg gatctgcttc
     2401 gtatgcatct tcgtcttctg gatcatctgc attgtagtca atggcatctg aatcaccatc
     2461 cataaaggca ttgacacgtt ttttcttagc ttttggtgct acttcatcgt cgtcattttc
     2521 ttcaagagcg atgatttctt cgtcgatttc gtccacacca taccatgaac gaagacccca
     2581 tttgttgtcc ccaagtgaga tgaagctacc gtcaaagttc aactctgtgt agaacagagg
     2641 caaagcttcg cggatatcgc tgtttgatgt tccaaggtag ttttgaattt cgtttacaag
     2701 atcgctaaaa tgcatctcat gatcgcgacc acgaagttct aatatagcac gcgctacctc
     2761 aatcatagat agttcacttt tttcttgccc agcaaatact tctaattcca aagcgtttct
     2821 cctcatttat actactatcg ccagagcgaa cagactctga cctcatttta tcatttactc
     2881 tttattttac gataattttg cggaatagtc aaaggttaag ggggagaaag tggcaggatt
     2941 agactaattc caatataaaa ctccttcctt tttctgttgc tccattttcc acaaatccaa
     3001 gcgacttgaa acacctccta gaagcatgat tgtaggtgta gatttccttg actctcaatt
     3061 ctttccatcc ttttactcga gccaattcaa tcaaagcact tagaatcttt tttccaagtc
     3121 ctcgatgttg gtaagcggaa ttcccaatca caatggggag attatcctga gatagtgtaa
     3181 tatccccaat tggaaaccat tctcccttct ccttgacttc aatccaaaaa agctcaccat
     3241 gccgatccaa ataggaatac atggcttcca aggtcgcttg actgtaagga agcttcaccc
     3301 catctacgag gtaaaccaag ttcacatcct gataccaagc aaaagcttcc tcacaatgat
     3361 tgaccttata ataaggaaca aaacacaatg cactaccttt tcaacaaaag agttgctgct
     3421 tgattaaaac catcacacca gttataccat tttgcttcat acccatcttg agctaggata
     3481 cgatcttcta aatcaaaaac agagtaaatc tttctttcct cgcaagcttg cgcatagaga
     3541 tgatatagtt catcaccacc atctctatcc cactcagcag aaatcgtatc ccgatctgcc
     3601 aataaagtct gataagccct gtgatgccca tctgtaatca gcaaacaatc tccaaaggca
     3661 agaatactga ttggatcgac ttggattgtt tctgccgact ggtaaagcat ctgaatatct
     3721 tgcaacttct tttctgataa atatagttga gtcggatgaa gatctgctat gttgactttc
     3781 atttctttct cctcaaggga attcgatact cacttctgtt tgcctttaaa tcgccattgg
     3841 aagcggagct tgtcatagaa gggaaactcg ataaacagga ctcccaagcc cacacagaga
     3901 ctggcaagga cgtctgatgg gtaatgaact cccagataga ctcttgatac cagcacactg
     3961 actaggtaga ggccaaggac gatttgtacg atttttctcc agactggatc tttaatccgc
     4021 tgactaagaa taacaatcaa agtacctacc atcaaggtta cagccagaga atgcccactt
     4081 gggaaggaaa atcccttctc ctccaccaga tgtaagatat ctggccgtgg gcgctggtag
     4141 atatttttaa aggtcacgat taaaagacct gccaaagcca gatttcccag catgaagaaa
     4201 ctttctatct tccatcgctt acgataaaag acaaaagctg taatgacaac ccaagtgata
     4261 atcactggga tatcaatcag acgtgtgagg gctcgaaaaa gaatagtcaa ataatctggt
     4321 aagtctcctc gaatggcagt ctgaatcgat tggtcaaaat tgaccagcgt ttcagggtaa
     4381 aatttgacca tgtagccaag aataacgaaa agtaaaaggg caaaactgcc cttcattaaa
     4441 aatgtttgtt tatctctcat aatgttttaa ggttggtttc aagagaacat acaacaacca
     4501 gaatgaaacg gaaaagataa caccttcaat caagttaaaa ggtaatacca tggtcattag
     4561 gtagttggaa agtcccaaaa tttttccaat atcaaagtta gcaaacttag cgtacaaagg
     4621 aacagcataa acatagttga gaaccaacat ggccaaggtt aaaccaatag ttccagctag
     4681 agagcctagt aggaaacgaa gggttgtccg ttcctttttc caaatcaaag caaatacgat
     4741 gacaaaaact cccaaagcta cgatattcat cggcaaacca atgtaagtat tcactccttg
     4801 gctgttaaga agcaacttca agagtgagcg aagcaagagc actcctagag cagcaggcaa
     4861 atccatgacc accagaccca caaggactgg caagatacta aattcgatct tgaggaaaga
     4921 tgccgctggt aaaagcggaa aatcaaagta catcagcaca aatgagatgg ctgatagaat
     4981 tgcaatggtc gaaagtcgac gtgtgtttgt cataacaggt tcctccaatt ttctataaaa
     5041 tcagaagaag ttggaaagga ttcctctatc tattctcact ttttatatcc caaaagttcc
     5101 ctcttactct attaaagaaa aacaaagcaa gtggttacaa tccggctata aatctatcaa
     5161 aacagacaaa gctattcttt cgtcttctcc catccagact atactgtcgg ttgtggaatc
     5221 tcaccacatc agcttgcgct cgcggacttc tttaaaggga aagaaataga gatttttctt
     5281 ttagactagg cgacaagctg taggcataac ttctggttag gcaaagcttg tcaacaaagt
     5341 ataaaaggaa aagatcattt cctagttaaa gtcaccgccg gtcgggaatc acaccctgcc
     5401 ctgaagactc ttttatcata acaaaaaacg cttgcaagcg caagcatttt gttcattttc
     5461 attttttatt tcaatttatc cacttcatag gtatggacga gctcaagacc tgcaaagttc
     5521 tgctggcgaa gagcctcata gacaatcatg cagacggtat tagacacatt gagactgcgg
     5581 acatgttcat cattcatggg aatacggaga gccttctcag gatgttctcg cataaaatct
     5641 tcaggtaagc ccttgtcttc acgtccaaag agaaaataat ggtcctcgtc agtcgataaa
     5701 tccacctcag aatagatttt ttccgcgaat ttcgaaatca gatagagttt tcccttcatc
     5761 tgagatatga aatcttctaa actctcgtaa aaataaatct ctagcttatc ccagtaatcc
     5821 aatccagccc gcttcatctt gcggtcatca ataggaaagc ccataggctt gatgatgtgg
     5881 aggggagaat tggtcgcagc gcaagtacgc gcgatattgc ctgtattttg tggaatttga
     5941 ggttcaaata atacaatgtg atttgtcatg acttgcttcc tttcaccatt gcaaaaaaat
     6001 agccacactg cccggagtca agctcagcaa acagcgtggt taaggcatcg ttaacttacc
     6061 tcacaacagg tttgaagtaa atcagcgaaa ctactttctt agtataacac tttcagaatc
     6121 attgtcaata gaaatgactt gattttttca attttttcaa gctatttcca agggttgtaa
     6181 aatcgtccct gattctgcaa gataagtagt aaactaacta ctaaaaacaa ggttgccaag
     6241 agcaaggtaa tatagtctcc ttttttcaag gcctgataac tataccatgt gcgttttttc
     6301 tctttcccaa agcggcgaag ctccatggca gtcgcaatgg tatcaatgcg ttctagcgag
     6361 ctaaaaatca agggcgtaat aatgagcaga ttgcctttga ttcgttgcat aagagaagct
     6421 ttcttggata attccatccc acgcgcctcc tgagacatct tgatagtaaa gaattcttcc
     6481 tgcaaatctg gaatatagcg caaggtcagg ctgacagaat aagcaatctt atagggcaca
     6541 ccaatttgat ttaaactgga agcaaactga ctaggatggg ttgtcatcaa aaagataata
     6601 gccagaggaa tggtgcaaag atacttaatg gccaaattta gcagataaaa gagctcctgg
     6661 ctggttagag tgtagacacc gattccctgc caaatcacac ttctctctcc ataaagtcca
     6721 accccatact cgggagaaaa gagatagacc atcaaaacgt ttaaaacggc aaataccgtc
     6781 gcaaaaacgg ctacaaagga aacatcttta aagcgaattt ctgataaata gaggagaaag
     6841 actgaaaaga tggcaatcag caagagcatt ctggtatcat agctaatcat ggccgccaat
     6901 gataccagaa tgaaaaagag aagtttccca gctcctgaca agcgatgaat cacagtatct
     6961 ctatgctggt aaccgattaa tttagcttgc atccctctct cctttctttg taaaatgccg
     7021 ttaaatccag tggatccaca tctagtttct tagccaagtt aaagatggag gtttctttta
     7081 gattggcttt tactaacagc tcaggatcgc tcaacagact ggctggaaca gtatcggcaa
     7141 tcaattctcc atccaccatg acaaggaccc ggtctgaata atccagcatc aattgcatat
     7201 catgggtaat catgacaatg gtatgccctt tttgatgtaa ctcttcgaga aattccataa
     7261 tctcagtata gttcttctga tcttgacctg cagtcggttc atctaggaga ataatttcag
     7321 ctcctaagac caaaattgaa gcaatggtga cacgtttttt ctgaccaaat gacagggcag
     7381 aaataggcca attacggaat tcataaagtc cacagatttt caaggtttca tagactctcg
     7441 tttcaatttc cttctcatcc acacctcgca aacggagccc tagagccacc tcatcaaaaa
     7501 tcatattggt tgaaatcatt tgattaggat tttgtagcac atagcctact cgttccgccc
     7561 gctctgcaac agaatcgcct tttatatcct gtttttccca aagatagcgt ccttccgtct
     7621 gaataaagct acttatagcc ttggctagag ttgatttccc tgctccattt tttccgacaa
     7681 tagcaatctt ttcacccttt ttaatatcta aatgtaggga ttttaaaatc ggtctatcat
     7741 cataagagaa agacacgtcc tctaatttaa agagtgactg caattctgga gtttcttttg
     7801 ccagttcatt ctgcagctga acctgacctt ttgagataga catgttatcc agattcgcta
     7861 actgttcttc cttgactaag tccacaccta attgacggag agtcgttaga taaaggggtt
     7921 ctcgaattcc attttgagtc aataaatcag tcgcaagcaa ctggtcaggg ctcccattaa
     7981 aaaggatacg accatcgctt atcaagacaa tccgatccac agggcgatgc agaacatcct
     8041 ccaaacggtg ctcgataatc agagtcgtcg tcccctcttc cttatgaatc tggtcaatca
     8101 attcgataat atcctgacct gacttgggat ctagattggc gagtggctca tcaaacaaga
     8161 gaatcggact ttcatcaatc aagacaccag ccagactgac tcgctgcttt tgtccacctg
     8221 acaaatcctg aggacgctga tccagtaaag gaagaaggtc cagcttttca gcccatttat
     8281 aaacacgacc tttcatctca tctagggctg tcacatcatt ttccagagca aacgccaaat
     8341 cttctgccac agacaagcca ataaactgcc catctgtatc ctgcaaaact gtgctaacca
     8401 gatgagactt atcatagatg ctcatatcaa aggctacttg accctttatc aaaaattctc
     8461 catatgtctg acccttgtaa atattgggaa taatcccatt caaacactga cccaaggtag
     8521 atttacctga cccagatggt ccaacaatta agactttctc tcccttgtaa atggtcaagt
     8581 ctatcccttg caaggtcggt tcttgttgtg tttcataccg gaaagagaaa tccttccact
     8641 caattatagc ttctttcatc ttactctctt cattcgcttc ttagacttct attttatcat
     8701 aaatcaagcc cttcttgcag tctcttctct taaaatctta gcgccaaaaa gattcctatc
     8761 ctagcttact tgcctaacta atctataaac atcgaaaaag actagttgcc cagccttccc
     8821 catcatttta tactcttcga aaatctcttc aaaccacgtc agcttcgcct tgccgtaggt
     8881 atggttactg acttcgtcag tttcatctac aacctcaaaa ccatgttttg agctgacttc
     8941 gtcagttcta tccacaatct caaaacactg ttttgagcaa cctgcggcta acttcctagt
     9001 ttgctctttg attttcattg agtattagtc ctttttcaaa cttcctgcac gagtttgggt
     9061 tcctgcatag gcaagtaaga gaagagttcc tgcaatagct acagatacac cattggcaat
     9121 tcccgcaaca atcccttgtg caaatacttt ttctgccgct tcttgataaa tcacaacatc
     9181 tccaagtggt gccaagacac cccaaacaag ggcatttgca agtagttgaa tgaggttaaa
     9241 aataagaata tctttccagt caaaaacacc attgatcact cgaacgtact ttctaaaaag
     9301 tcccacaact agaccaaaga gtccgctagc gataatccaa gtccaccata gaccataacc
     9361 agcaagagag tccttgattg catgaccaat caacccgaca agcaaaccga taatcggtcc
     9421 aaaaataata gaaagtagcg cttgtaccgc atactgaagc tggatgcttg tatttggaac
     9481 aggggttgga atgttgatca tcccgatgac gacaaagagg gcagcgccaa ttccgacagc
     9541 aacaacttgt ttaattgtaa atttgatttc catactattc tcctatttta tccttctatt
     9601 ttctttattt caatggtcca agatgaaccg acacctacat tataggcctt ggcaaaggaa
     9661 ccttggttga tagccaaacc taaacgatag agagagttga tgtaaaggat gggttgccca
     9721 attctcacat ctgcaaatga tttgccatag acaacctgat tttgatagac cagcatatca
     9781 gcatgataga tggtcacttc aaaacgatca ccaaattctg gttccagctt gtaaaattct
     9841 tcccgtgtga tagaggtcca aagcgaaccg aaacgcacat ccagaatatc aatggctccc
     9901 ttcaccagat gatcttctat gatggtcgct acgactggaa gctctacaat ctgttccaca
     9961 ctgagctctg gccctacttc ctcaaaagta atgtgaccac tggccagttt agcaccagta
    10021 taggcataga catcacgacc gtggaaggta taagaatgct ctgtgttttg acgcctattg
    10081 gccacctcag aaatctcacg aatggctaca atgccaacgt gtttcttgat aaaggaaagc
    10141 gtcccattat ctggcgtgac aatgtattga ttttttgcag tcttggcaac tacactctta
    10201 cgtttcgaac cgacacctgg atcgacaacc gatacaaacg tcgttccctc aggccagtaa
    10261 tccaccgtct gaaagagacg atagctcccc tcaaaaatat tataaggcgt gatatcgtgc
    10321 gtcaagtgat ggatttttaa ggttggagac tcttctaaag ccactccaat catagccgac
    10381 accgcaccat caaccagacc aaagtctgat tgtaatacca gtaaattatt gttcatttta
    10441 tctccttgta tatcctttat cataccatat ttcagagaaa gtcgctaata gctatttcaa
    10501 ttggttaggg tatagtttta ccttatagcg aattttcact aaaagctctt gttattagct
    10561 agtaggtgca ttttttcttg aaaaagggta tgatagttac atcatgaaaa agtaggtttt
    10621 attatgaaaa ttatccttgt cggaggggga aaagttggtt ttgccctctg tcgctccttg
    10681 gttgcagaaa agcatgatgt tttgctgatt gagcaagacg aagctgttct caatcatatt
    10741 gtcagtcgct ttgatatcat tggtatcctt ggtaacgggg ccgattttgc cattcttgag
    10801 caagccagcg tccaagattg tgatatcttt atcgccctga ctgagcacga tgaagtcaac
    10861 atgattgcag cagttctagc caagaaaatg ggagctaaag aaactatcgt tcgggtgcgg
    10921 aaccctgaat attctaactc ttatttcaag gaaaagaata ttctcggttt ttctcttatc
    10981 gttaatcctg agctcttggc tgcccgcgct atcgcgaata tcattgactt ccccaacgcc
    11041 ctgtctgtcg aacgctttgc tggtggacgc gttagcctca tggaatttgt cgtcaagtcc
    11101 accagcggtc tttgccaaat gcccatttct gattttcgta aaaaatttgg taatgtcatt
    11161 gtctgtgcga tagagaggga tcatcaaatt atcattccaa gcggtgacat gactgtacag
    11221 gataaagata gaatctttgt cactggtaac cgtgtcgata tgatactctt ccataattat
    11281 tttaaatcac gcgccgtgaa gagccttctc atcgttggag caggtagaat tgcctattat
    11341 ctacttggta ttctcaaaga tagtcgtatc gatacaaaag tcattgaaat caatcctgaa
    11401 atcgccagct tctttagcga gaaattccca aatctctaca tcgttcaagg agatggaacc
    11461 gcaaaagata tcctgctgga agaaagtgct caacactatg atgccgttgc gactctaaca
    11521 ggagtcgatg aggaaaatct gattacatct atgttccttg acagggtagg tgtacagaaa
    11581 aatattacta aggtcaatcg taccagtctc ctcgagatta tcaatgcgcc tgatttttca
    11641 agtatcatca cacctaaaag catcgctgta gatacgatta tgcactttat tcgtggtcga
    11701 gttaatgccc agtattcaga ccttcaagcc atgcaccatc tagccaatgg ccaaatcgaa
    11761 accctgcaat tccatatcaa ggaagccaat aaaatgactg ccaaacctct ttctcaactg
    11821 aaattgaaaa aaggggttct tattgcagcc attattcgaa agggcaagac tattttccca
    11881 actggggagg atatgttgga agttggagac aagctcctag taacaacctt gttgccaaac
    11941 atcaccaaga tttatgactt gattgcgagg taagaaatga ataaaagtat gattcgttac
    12001 ctcctttcaa agttactttt gattgaagct gttctcctct tggttcctgt ggctatcgct
    12061 gtctattacc gtgaatcgag ccaagtcttt acagccctct tttcgaccat agggattctc
    12121 gtattgttag gcggttcagg gagtttacag aagccaaaaa atcaacggat ttatgccaag
    12181 gagggagtct tgatcgttgc cctctgttgg atcctttggt ctttctttgg tggtctcccc
    12241 tttgtctttt ctgggcaaat ccctagcctt attgatgcct tttttgaaat tagttctggg
    12301 tttacaacta ctggagcaag tattttgaac gacatttcgg ttctcagccg ttccctcctc
    12361 ttctggcgaa gttttaccca cttgattgga gggatgggag tgcttgtttt tgcacttgct
    12421 attatggaca atgccaaaaa tagtcaccta gaagtaatga aggctgaggt tcctggtcct
    12481 gtctttggta aggttgtatc caaattaaaa aacactgccc agatcctcta tctcctttac
    12541 atagccctct tttccctctt tgtcatcatc tattatctag ccggtatgcc tctctacgat
    12601 agttttgtca ttgctatggg gacagcggga actggaggat ttaccgtcta taacgatgga
    12661 attgcccact atggtagctc actgattacc tatctggtta gtataggagt tttggttttt
    12721 ggggtaaatt tcaatctcta ctactacctt atgctccgtc gggttaaggc cttctttgga
    12781 gatgaagagc ttcgggctta cttggtcatc gtgctggttt ctacaggctt gattagcctc
    12841 aatactctct acctctaccc agggttttca aagagctttg aaatgacctt cttccaggtt
    12901 tccaacatca ttacaacaac tggttttgga tatggagata ttaccaactg gcccctcttc
    12961 tcccagttta tcctcctttt cctcatggca atcggtggct ctgctggatc aactgcaggt
    13021 ggactcaaga ttattcgagg cctcatcctt tcaaaaattg ccaaaaacca aattttgtca
    13081 atcctatcgc cccaccgtgt tttgaccctc catgttaata aaatggtgat tgacaaagat
    13141 acccagcata agattctcaa gtactttgtc atctatgcta tgattttgct agcgcttatc
    13201 tttattgtca gcctagatag caatgatttt ctggtcgtga ccagcgctgt ctttagctgt
    13261 ttcaataata tcgggcctat tctaggaacc acttctagtt tctcaatctt tagtcccatc
    13321 tcaaaaattc tcctctcctt tgcaatgatt gcagggcgct tggagattta cccaatcctc
    13381 cttctcttta tgaaaagaac ttggtctaag agataaaata caaaatccct ctttttacaa
    13441 ttgtgaaaga gggattttta tattaaaaag ggggaacgtt tttaaatcca agcctgtctg
    13501 taagcaaagt tcttactcaa ataaaagatg gaaaaactta ctacacagct tggtaatgct
    13561 ccaatacaaa ttgtaaggct ttacttggat tctttgttaa atgaatatat tccattcctc
    13621 tggtagcaac tatctgaata cctaatcttt ctgcatccac cttcatctct ctataataac
    13681 tccgaacttg tggagccaga ataattaaat cataaacacc cataatatca taatgagctc
    13741 cgtacgctcc tgaattcgca atcactctaa cttctgctaa ttgagcgccc tcgttaattg
    13801 cattggctaa ttacgcactt gttccagacc ctgcacaaag aaccagtact ttcaactcct
    13861 tactttttat tacatctatc tcaccaggca ttatttcact tgtatcctcc tctaaaataa
    13921 tatcatttga gcttgcaata tctacaacct gtagtagata tctcgtattt agacaatatg
    13981 aaaacaagac gacttccata taggaaaccg ccttctctct atgttgagtg atttatatta
    14041 aaataacttt tcttctagct gcattttatt attataaaaa cattcatcat aaccctgaac
    14101 ttaaataa
//
LOCUS       DAVLFB010000046        13916 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLFB010000046 DAVLFB010000000
VERSION     DAVLFB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.046"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..1717
                     /locus_tag="VXD58_001228"
     CDS             <1..1717
                     /locus_tag="VXD58_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV0675931.1"
                     /translation="REDNIKGHRTYDAFSEIISKTTLSENLVNFLNYNRRLFTADVNI
                     YDWFKKATEGNVYIAERASEVDEIKAAKYKVWDNLQSATHRKMILPLLNLNKSHVYLI
                     SNYSAMAVGTAEKLGHSSFDGIKNQIEKAADSYRNYFDFWYRLSLDKVKNMLVKNTVT
                     PLWEGYQAPGGWPDKYGKRNNKEDYAPLRELFGPIGKYYGNNGTGAYAVIWDNPLDTR
                     TEVNYIALSMIDEFGISVYTHETTHVNDRAIYLGGYGRRSGTHAEAYAQGMLQTPVPS
                     TWFDEYGALGINMTFYRPNDGNQWYITDPKTLKTREDIDNYMKGYIDTLSLLDYVEGD
                     AVISKHDDKLNQLWFKKVDPIERTVGSKDQYDKVRNMSSDDWKGITLNSVNDLVDNNL
                     MTNRMMNNNIYKPEVFDPGIAYVGIKMMNSIYAGNTSEGAPGAVTFKNNAFRLWGYYG
                     YDKGFLGYASDKLKSQANKEGKQFGDQYIIQKISDGRFQSFADFKKEYYKELLEKANA
                     GFTPFTIDGETIHNYDELKSLFAKALEEDMVTNKVNGKLVQVKEKVYKKLLQQTNSFK
                     TSIFK"
     gene            1778..3499
                     /gene="pabB"
                     /locus_tag="VXD58_001229"
     CDS             1778..3499
                     /gene="pabB"
                     /locus_tag="VXD58_001229"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV0675932.1"
                     /translation="MHRKTVIDFRTLGERYTFTKPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            3500..4117
                     /locus_tag="VXD58_001230"
     CDS             3500..4117
                     /locus_tag="VXD58_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0675933.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            4167..5165
                     /locus_tag="VXD58_001231"
     CDS             4167..5165
                     /locus_tag="VXD58_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0675934.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            5292..6251
                     /locus_tag="VXD58_001232"
     CDS             5292..6251
                     /locus_tag="VXD58_001232"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV0675935.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            6348..7187
                     /locus_tag="VXD58_001233"
     CDS             6348..7187
                     /locus_tag="VXD58_001233"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV0675936.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(7235..7405)
                     /locus_tag="VXD58_001234"
     CDS             complement(7235..7405)
                     /locus_tag="VXD58_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV0675937.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            7526..8410
                     /gene="miaA"
                     /locus_tag="VXD58_001235"
     CDS             7526..8410
                     /gene="miaA"
                     /locus_tag="VXD58_001235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV0675938.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVSHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            8403..9641
                     /gene="hflX"
                     /locus_tag="VXD58_001236"
     CDS             8403..9641
                     /gene="hflX"
                     /locus_tag="VXD58_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV0675939.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMKELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIAFMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESITFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            9634..10257
                     /locus_tag="VXD58_001237"
     CDS             9634..10257
                     /locus_tag="VXD58_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV0675940.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            10272..11201
                     /locus_tag="VXD58_001238"
     CDS             10272..11201
                     /locus_tag="VXD58_001238"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV0675941.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQNLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            11222..11977
                     /locus_tag="VXD58_001239"
     CDS             11222..11977
                     /locus_tag="VXD58_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV0675942.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(12031..12939)
                     /locus_tag="VXD58_001240"
     CDS             complement(12031..12939)
                     /locus_tag="VXD58_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV0675943.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            13003..13272
                     /locus_tag="VXD58_001241"
     CDS             13003..13272
                     /locus_tag="VXD58_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV0675944.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            13272..13652
                     /locus_tag="VXD58_001242"
     CDS             13272..13652
                     /locus_tag="VXD58_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV0675945.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         4204 a         2549 c         3157 g         4006 t
ORIGIN      
        1 aagagaagat aatataaaag gtcatcggac atatgatgct ttcagcgaga ttatttctaa
       61 gacaacctta tcagaaaatt tggtaaactt tttaaattat aatcgtcgac tatttactgc
      121 agatgtaaat atttatgatt ggtttaagaa agctacagaa gggaatgtct atatagcgga
      181 gcgtgcttcg gaagtcgatg aaattaaagc ggcgaaatat aaagtttggg ataatttaca
      241 atctgctact catcggaaga tgatactccc attattaaat ctaaataaat cacatgtgta
      301 tttaatctcg aattatagtg caatggctgt tggaacagca gaaaagttag ggcatagctc
      361 cttcgatgga ataaaaaatc agattgaaaa agcagcggac agttatcgaa attattttga
      421 tttttggtat agattatctt tagataaggt taagaatatg ctagtcaaaa atacagtaac
      481 tcctctttgg gaaggttatc aagcacctgg tggatggcct gataagtatg gtaagaggaa
      541 taataaagaa gactatgccc cattgagaga gttgtttgga cctattggta agtattatgg
      601 taacaatgga acaggtgctt atgcagttat ttgggataat cctttggata cgcgaacaga
      661 agtaaattat attgcattaa gtatgattga tgagtttgga atctctgttt atacacatga
      721 aactactcat gttaatgatc gtgcaatcta tttaggagga tatggacgtc gctcagggac
      781 tcatgcagaa gcctatgcac aaggaatgtt acaaacacca gtaccaagta catggtttga
      841 tgaatacggt gctttgggaa ttaatatgac tttctatcgt ccaaatgatg gaaatcagtg
      901 gtatattacc gatccgaaga ctcttaaaac aagagaagac attgataatt atatgaaagg
      961 ctatatagat accttgtctc ttcttgatta tgttgaaggt gatgcagtca ttagtaaaca
     1021 tgatgataaa ttaaatcaac tgtggtttaa gaaagttgat cctattgaac gcactgtagg
     1081 gtcaaaagat caatatgaca aggttcgaaa tatgagctct gatgattgga aaggaataac
     1141 attaaacagt gttaatgact tagttgataa taatctgatg actaatcgta tgatgaataa
     1201 taatatatat aagccagaag tatttgatcc aggtattgcc tatgtaggaa ttaaaatgat
     1261 gaatagtatc tatgcaggta acacaagtga gggtgcaccc ggtgctgtta catttaagaa
     1321 taatgcattt agactctggg gatattatgg ctatgataaa ggattcttag gatatgcttc
     1381 agataaatta aaatctcaag ctaataaaga agggaaacaa tttggagatc aatatattat
     1441 acaaaaaata tctgacggca gattccaatc gtttgctgat tttaagaaag aatattataa
     1501 agaattgtta gaaaaagcca acgctggttt tactcctttt actattgatg gtgagaccat
     1561 tcataactat gatgaattaa aatcattatt tgccaaagct cttgaagaag atatggttac
     1621 taataaagtt aatggtaaac ttgtgcaagt aaaagaaaaa gtttataaga aacttcttca
     1681 acaaacaaat agctttaaaa cttcaatctt taaataattg aaatctctca tctgctttgc
     1741 ggatgagggt tttcttattt tatgctatac ttaaggtatg catagaaaaa cagtgattga
     1801 ttttaggact ttgggggaga gatatacctt taccaagcct attaaagagt tgaaaacgag
     1861 aaatgtagaa gaagtggcag atttgctggc acaagtggaa agctaccaag agcaaggtta
     1921 ttatgtggtg gggtatgtca gctacgaggc tgcacctgct tttgaggaaa aattagcagt
     1981 tcacaaggat cccctactgg gagagtacct gctttatttt actgttcatg atagcgtaga
     2041 aacttcccct attcctctga cttatgagga tattgatttg ccctcaaatt ggcaggaagt
     2101 aacgtctgca gcagactatg aaaaggccat tgcccaaatt caccatcatt tacggcaggg
     2161 agatacctat caggtcaact acaccgtcca acttaagcaa aagttaaatg ccaatccttt
     2221 tgccatctac aatcgtatgg tggtagagca ggaggcgggc tacaatgcct atgtggaaca
     2281 tgacgagatg gcagtgattt ccatgagccc agagctcttt tttgagcaaa atgatcgcga
     2341 gttgacaaca cgaccaatga aggggacgac tcagcgtggg gtaactgacc aagaagatct
     2401 tgaacaggcc agttggttgg aacaggatcc caaaaatcgc tctgaaaata tgatgattgt
     2461 ggacctcttg cgcaatgata tgaaccgtat ttctgaagtt gggagcgagc acgtggagcg
     2521 tctgtgtcag gtagagcagt attcaactgt ttggcagatg acttcgacca tcaagagtca
     2581 gttgcgagag gatgtggacc ttgttgaaat cttccgctca ctctttcctt gtggttccat
     2641 aacgggtgca ccgaaaattg cgacaatgga gattatcaag gacttggagc ctcaaccgcg
     2701 tggagtctac tgtggaacga ttggtctctt gcttccaaat ggacgacgga tttttaatgt
     2761 ggccattcgt accattcaac ttcacaaagg tcaagccatt tatggagttg gcggagggat
     2821 tacttgggat agcacatggg aatctgaata ccgagaggtt catcaaaagg cagcagttct
     2881 ttatcgtaaa caagctcgct tccaactgat tacgacaggg gaaatcagcc agaagaacct
     2941 gttgtttgaa gatcaacatc tggaaagact gagaaaagct agtcgttatt ttgcctttcc
     3001 ttttgatgca gaagacttgg gacacaagat tgaggaagag tgtcaggatt gtgaagctaa
     3061 tcgagattac cgcttgcgaa tcagccttag caaatctgga gagatagaag tcaatcgtca
     3121 agtattaacc cctctcagta caagcttttg tcaggcccaa gtctgccttc aggaagctgc
     3181 tttgaatcaa tcctttacct actttaaaac cactcaccga ccgcatttga gcctaggaga
     3241 acaagagaag atttaccaca ataagtcagg agaactgctt gaaacctcta taggaaattt
     3301 ggttctgaaa atcgctggaa aactctacac accgcctatc cgacttggaa tcttgccagg
     3361 aatttaccgt cagtatttgc tagaaacagg acaggtagaa gagaaagtct tgaccttggc
     3421 agatttagcc caagcagaag ctatttacgg ctgtaatgca gtgagaggct tgtatgaact
     3481 aagccttgag gagaactaga tgaaactgat atttttgcat gggttagggc agtcagcaga
     3541 gtcttggaaa gaagttcgga atctactgac agattatcct tctgaggcta tcgagttatt
     3601 tccttctgga gttagtaact atcaacaagc taaggagcga gtttatcagc acctagctca
     3661 agagacagaa ccttttgttt tgattggatt gtccttaggt gctgccctgg cactagaact
     3721 ttccagttac gatttaccaa atcttcgagc tttgattctg tcaggatgcc cgcttaaatt
     3781 agcagggaat atcctatttt atcttcagtt gctgattttt aaactacttc ccaaaagagt
     3841 atttgaaaaa cagggagcag acaaggctct tatggtcgga gtttctgagg agttgaagac
     3901 acttgattta acggatatag caggaacttg cccttatcca accttactaa tttgtggtat
     3961 caaagataaa ccgaatctta gttctatgag aagtcttcat aaactaatat cagaatccca
     4021 atttcagatt atcccagatg gccctcatgt cttgaataag gcaaaaccaa aggagtttgt
     4081 agaaaagacc agaagtttcc ttgaattgct gaaataagtt tgataaaata atattgaaat
     4141 cgatgtcata attatgggag aaagaaatga ataaacgtct atttttaaaa atgagtctgg
     4201 tgacgttgcc aattttagcc ttgttttcac aacctgtgtt agcagaggaa aacattcatt
     4261 tttcgagttg taaggaagct tgggctaatg gctattcgga tcttcatgag ggagaacctg
     4321 gttattctgc caagttggac cgcgatcatg atggcgtggc ttgcgaattg aaaaatgctc
     4381 ctaagggtgc ttttaaagca aaacagtcaa cggctattca aatcaacaca agttcagcaa
     4441 caacaagtgg ttgggttaag caggacggcg cttggtacta ctttgatgga aatggaaatc
     4501 tagtgaaaaa tgcatggcag ggaagctatt acctgaaagc tgatggtaaa atggcacaga
     4561 gtgaatggat ttatgactct tcttatcaag cttggtatta tttgaaatca gatggttctt
     4621 atgcaaaaaa tgcatggcaa ggagcttatt accttaaatc aaacggtaaa atggcacaag
     4681 gtgagtgggt ttatgattct tcttaccaag catggtatta cttgaaatca gatggttcat
     4741 atgctcgcaa tgcatggcaa ggaaactact atttgaaatc agatggtaaa atggctaaag
     4801 gtgaatgggt ttatgatgcc acctatcaag cttggtatta tttgacatca gatggttctt
     4861 atgcttacag tacatggcaa ggaaattact atctaaaatc ggatggtaaa atggctgtca
     4921 atgaatgggt tgatggtgga cgttattatg ttggcgctga cggagtttgg aaggaaggtc
     4981 aagcaagtac agcttcttct agtaatgata gcaatagtga atattctgct gctttaggaa
     5041 aggcaaaaag ttataattcg ttattccaca tgtcaaaaaa acgtatgtat agacaattaa
     5101 cttctgattt tgataaattt tcaaatgatg cagctcaata tgccattgat catttagatg
     5161 attaacagat actggtaggc gaaaaaattc cttataattc atttacgcaa acctttgcat
     5221 aagtgaatgg attttgatat actagtactg taagcatttt caattactac acaatgaaaa
     5281 aggagaatat gatgagtcaa aagattattg ggattgacct tggtggaact tctatcaaat
     5341 ttgcaatctt aacaacagca ggagaaatcc aaggaaaatg gtcaatcaag accaacattt
     5401 tggatgaggg aagtcatatc gttgatgata tgattgagtc tattcaacat cgtttggact
     5461 tgcttggatt ggcagcagca gacttccaag gtattggaat gggatcacca ggtgttgttg
     5521 accgtgaaaa agggactgtt atcggtgcct acaacttgaa ctggaaaacc cttcaaccaa
     5581 tcaaacaaaa gattgaaaaa gctttgggca ttccattttt catcgataat gatgccaacg
     5641 tagcagctct tggtgagcgc tggatgggtg ctggtgataa ccaaccagat gttgtcttta
     5701 tgacactcgg tactggtgtt ggtggtggta ttgtcgcaga aggcaaattg cttcatggtg
     5761 ttgctggtgc agcaggtgaa cttggtcata tcactgttga ttttgaccaa ccaattgcat
     5821 gtacttgtgg taaaaaaggc tgccttgaga cagttgcttc agcaacagga attgtcaact
     5881 tgactcgtcg ctatgccgat gaatacgaag gcgatgcagc cttgaaacgc ttgattgata
     5941 acggagaaga agtaactgct aagactgtct ttgatctcgc aaaagaagga gacgaccttg
     6001 ctttgattgt ttaccgtaac ttctcacgtt acttgggaat cgcttgtgct aacattggtt
     6061 caatcctaaa cccatcaaca atcgtcatcg gtggtggtgt gtcagctgcg ggagaattcc
     6121 ttctacaagg tgttcaaaaa gtttacgatg aaaatagttt cccacaagta cgcacatcta
     6181 ctaaattagc tcttgcaact ctaggaaatg acgctggagt tatcggagca gcatcacttg
     6241 tattgcaata agataatgag agaggaaaat gctaacctag agttagtaat gttcctcttt
     6301 tattttttat gctatactag ttaggacaat gaatgaggtg agagtaaatg acaaaagcag
     6361 atacgatttt taaagagaat attgaacgca ttctaaagga aggtgttttc tcagagcagg
     6421 ctcgtcctaa gtacaaggat gggactgttg ctaattccaa gtatgtaact ggtgcctttt
     6481 ccgagtacga cttatctaaa ggagaatttc ctatcacaac cttgcgtccc attgcaatca
     6541 aatccgccat caaagaagtt ctctggattt accaagacca gtcaaatagc ttagaagtgc
     6601 ttaatgacaa gtacaatgtt cactactgga atgactggga agttggagac acgggaacca
     6661 ttggtgagcg ctatggtgcc gttgttaaga aacacgacat tatcaataag cttctcaaac
     6721 agttggaaac caatccttgg aaccgccgca atattatttc tctctgggat taccaagctt
     6781 tcgaagaaac agatgggctg ctcccgtgcg cctttcagac catgtttgat gttcggcgtg
     6841 ttgatgggga aatctatctg gatgcgacct tgacccagcg ctccaatgat atgctggtgg
     6901 cccaccacat caacgctatg cagtatgtgg ctttgcagat gatgattgcc aaacattttg
     6961 gctggaaggt tgggaagttc ttctacttca tcaacaacct ccatatctat gataatcaat
     7021 ttgaacaagc tcaggaattg ctccgtcggg agccgtcaaa ctgccaacca cgcttggttt
     7081 taaatgttcc tgatgggact aatttctttg atatcaaagc agaagatttt gagttggtgg
     7141 attatgaccc tgttaagcca cagttgaagt ttgacctagc tatttaaaag aatagaaaaa
     7201 agaagttgag aataatccca acttcttttg tttcttaacg tgatacgcgg cgacgagctg
     7261 cttttttacg gttttcttcg atgaaagctg ctttttgctc ttctggttcg attactttct
     7321 ttttaaatgc gtatactgca cctgcaacgg cagcgacagt tcctgcgaca cctgttacaa
     7381 gacctttagc gaatccttta gccatgagtc ttcctccttt atattctcaa tcagccagcc
     7441 tcctcaagag gtcacatttt tctgactgac ctttttgtgt tataataata gtaacgaaaa
     7501 aatgggaatt tttcaaggaa aaaagatgaa aacaaaaata attgtgattg ttggaccgac
     7561 tgctgttgga aagacggctc tagctattga agttgcaaag cgttttaatg gcgaagtggt
     7621 tagtggagat agccaacaag tctatcgagg acttgatatt gggacggcca aggctagtcc
     7681 agaagagcag gcagctgttt ctcatcattt aatcgatgtt agagagataa ccgagtctta
     7741 ctcggctttt gattttgttt cagaagctaa gatgactatt gaggatattc acagccgtgg
     7801 caagctagcc attatcgccg gtgggactgg actttatatc cagagcttgc tagaaggtta
     7861 ccacctaggt ggggagactc ctcatgagga gattttagct tatcgagcta gtttggagcc
     7921 atattcagat gaggaattag cccatttggt ggatcaagca ggccttgaga ttccccagtt
     7981 taatcgtcgt cgtgctatgc gtgccttaga aattgcccat tttggtcagg atttggaaaa
     8041 tcaagagatt ttgtatgaac cgctgattat ctgcttggat gatgaacgta gtcaacttta
     8101 tgagcgtatc aaccaccgag tggatttgat gtttgaggct gggcttttgg atgaggccaa
     8161 gtggctgttt gaccattccc ctaatgtaca ggctgctaaa ggaattggct ataaggaact
     8221 ctttccttat ttccgtggag agcagacctt ggaggaagct agtgagagtc tcaaacaggc
     8281 gacccgtcgt tttgccaagc gtcagctgac ctggttccgt aatcgcatgc aggtcacctt
     8341 ttatcagatt ggagaatctg gtgtacaaga ccgcatttta agccagatag aggagttttt
     8401 agatgattga aacggagaaa aaagaggagc gagtcctgct gattggtgtg gaattgcagg
     8461 gtatggacag ttttgacctc tccatgaaag aattggctag tttagcgaaa acggcagggg
     8521 cagtcgttgt agatagctac agacaaaaac gtgaaaaata tgattccaag accttcgtcg
     8581 gctctggtaa gttggaagag attgcgttta tggtggatgc agaagaaatc actactgtca
     8641 tcgtcaacaa ccgtctgacc ccaaggcaga atgtcaatct agaggaagtt ctcggtgtta
     8701 aggtcattga ccgtatgcag ttgattttgg atatctttgc catgcgggct cgaagccatg
     8761 aagggaagct ccaagtccac ctagcccaac tcaaatacct cttgcctcgc ttggttggtc
     8821 aggggattat gctcagccgt caggcagggg gaattggttc ccgtggtcct ggtgaaagcc
     8881 aactggagct gaaccgtcgt agcgttcgca atcaaatcac ggatatcgag cgccagctca
     8941 aggtggttga gaaaaatcgt gcgactgtca gagaaaaacg tttggagtct atcactttta
     9001 agattggttt gattggttat accaatgctg ggaaatcaac tatcatgaac atcttgacca
     9061 gtaagaccca gtatgaagca gatgagctct ttgcgactct ggatgcgaca accaagagta
     9121 ttcatctggg aggcaacctc caagtaactt tgacagatac cgttggcttt atccaagatt
     9181 tgccgacaga gttggtgtcc agtttcaagt caaccttgga agaaagcaag catgtggacc
     9241 ttctggttca tgttatcgat gctagcaatc cttaccacga ggaacatgaa aaaacggttc
     9301 tctccatcat gaaagacctg gacatggaag atattcctca cttgacgctt tataataaag
     9361 cggatttggt ggaggatttc acgcctaccc aaacgccata taccctcatt tctgccaagt
     9421 ctgaggacag tcgtgaaaac ttgcaagcat tattgctaga taagattaag gaaatttttg
     9481 aagcatttac cctgcgagtg cctttttcaa agtcctataa gattcatgat ttagagagtg
     9541 ttgcaattct ggaagaacgt gattatcagg aagacggcga agtgattaca ggctacattt
     9601 ccgagaaaaa taaatggagg ttagaagaat tttatgactg atattaaaac tttggctcta
     9661 aagtatggag gttatacaag tctggacaag gtctatctgg atcagcttct agctggtaag
     9721 acagagcagg agcagttaac acttattaca cctccgccaa gtgttgttaa tgcctacttt
     9781 gcagaactct atcagaaaaa aagtcctcag gctgcgacgg attattttgc agaactcagt
     9841 caggaactga atctttacaa tactgagcca agtttcaccc tagaaaataa gtcttttatt
     9901 cggcttaatc tgtctggtaa atcctttggt ttttgttatg agagtgacgg ctttggtcgg
     9961 attttctctg aaaatgagga gaaaatatcg gatgacttgc tctttgaaat tgcgcaaatt
    10021 ttcccccatc aactagtctt tgaagagtct ggtaagattt acatgaagcc tgtcgaggac
    10081 gaggaagttg tcagcctaga aagtctcaca gctttgactg atttggaaag attggctgat
    10141 ggtcgaaaac gtctcaaagg ctacagccaa gaggatttat tgcaagaagc tgctgctttt
    10201 tctggcaaac gctatttccg atcggaaaac cgcacagcca tgttatatat tgattaatta
    10261 gaaagtatcg aatggatatt caatttttag gaacgggggc tggtcagccc tctaaagctc
    10321 gcaacgtttc aagtctcgcc ctgaaacttt tggacgagat taacgaagtt tggctctttg
    10381 actgtggaga aggtacgcaa aatcgcattc tggaaaccac aattcgacca cgtaaggtca
    10441 gcaaaatctt tattacccat ctgcatggag accacatttt tggtttgcca ggtttccttt
    10501 ctagccgtgc ctttcaggcc aatgaagagc agacagattt ggaaatctac ggacctcaag
    10561 gaatcaagtc atttgtctta accagccttc gtgtgtcagg ttctcgtctg ccctaccgca
    10621 ttcatttcca tgagtttgac caagattctc tgggtaaaat tcttgaaacc gataaattca
    10681 ctgtgtatgc agaggagctg gaccacacta ttttctgtgt tggctatcgt gtcatgcaaa
    10741 aggatctaga agggacgctg gatgctgaaa aactcaaggc tgctggtgtt ccgttcggcc
    10801 cgctttttgg taaaatcaaa aacggccaga atcttgtttt ggaagacgga actgaaatca
    10861 aggcagcaga ctatatctca gcgccacgtc caggtaagat tatcactatt ttaggagaca
    10921 ctcgaaaaac ggatgccagt gtgcgtctgg ctgtcaatgc agatgtccta gttcatgagt
    10981 ccacttatgg caagggtgat gaaaaaattg ctcgtaacca tggtcactca actaatatgc
    11041 aagctgcaca agtagcggta gaagcaggtg ccaaacgcct cctactcaac catatcagtg
    11101 cccgtttcct ctcaaaagat ataagcaaac tcaagaagga cgctgccaca atttttgaaa
    11161 atgtccatgt ggtcaaagac ttggaagaag tggaaatcta gcagtcacag aaaggataag
    11221 tatgcctact attctcatta ccggagctag cggtggtcta gctcaagaaa tggtcaaact
    11281 cctgcccaat gaccaactca tcttgcttgg tagaaataag gaaaaattag cccaactcta
    11341 cggaaattat tcccatgcag aattgattga aattgatatt accgacgatt cagccctaga
    11401 agctctggta actgatcttt atctccgcta tggcaagatt gatgtcttga ttaacaacgc
    11461 tggttacggg atttttgagg gatttgacca gattgctgat aaagatattc accagatgtt
    11521 tgaggtcaat acctttgccc tgatgaatct gtctcgtcac cttgcggctc gtatgaagga
    11581 aagcagcaaa gggcatatca tcaacatcgt cagcatggca ggtctaatag ctactggcaa
    11641 gtctagtctt tactcagcga ccaagtttgc ggctattggt ttttcaaatg ctctgcgact
    11701 cgaacttatg ccctatggag tctatgtgac aacagtcaat ccaggtccaa tccgaacagg
    11761 attttttgac caagctgacc cagatggaac ttatcttaaa tcggttgacc gcttcctctt
    11821 agaggcagat gcagtggcta aaaagattgt caagattata ggcaaaaata aacgagaact
    11881 caatctcccg attttgttga acctagccca taagttttat actctctttc ccaagctagc
    11941 tgataagttg gcaggggaaa cttttaatta taagtaaaaa gaaccaatgt gcaggttgtt
    12001 gctagcctac atattggttc ttatcatttc tcaactatca aactgaactt cttctagtag
    12061 atagtcgata aagcgttcgc ccatcttaga taggctggtt ttctcatgct ggatatagac
    12121 cagctcaatc gggtcatcaa tatccagtgg gatagaaacg atattgtctc cgtttaggtt
    12181 gctgttcaaa atccctgtcg caatggtata accatccaaa ccaatcaaga gattaaagag
    12241 ggtggcacgg tcactgacca caatggattt cttgtggtgt tcttgagaaa gaatctcttc
    12301 tgaaaagtag aaggagttgt gcgtcccttg gtcatagctg aggtaaggga aattctccaa
    12361 atcagacagt ttcaccttgt ctttctttgc cagagggttg gtcttgctga caaagatatg
    12421 cggttgcgct gtgaagagat ggtgggctag caggtgattg tcatccagca tcttggttaa
    12481 aacatcacgg ttgtaactgt ttaagaagag gaccccgacc tcactgcgga agttcttgac
    12541 gtcgtcgata atctcccaag tccgagtttc acgaaggaag agttcgtatt tctccatatc
    12601 gcttttcttg agcaaagaga caaaggcatt gaccacaaag gcatagtgtt gagacgaaac
    12661 gctaaagagt tcgcggtggg cgacaggatt tttatagcgt tcctccagaa gctgggtctg
    12721 ctcgacaacc tgacgggcat aagagagaaa ctccatccca tcacgggtca aggtgattcc
    12781 cttgggattg cggataaaga tctcaatgcc catttcattt tccaaatctc tcacagcatt
    12841 ggaaagactt ggctgagtga taaagagttg cttggctgcc tcattcatgg agccagtttc
    12901 gacgattttg ataatataat gtaattgttg aattctcatg tttttattgt accacacttg
    12961 ccttagaatc gctaaagaag ataggaaaat aaggagcatt gtgtgagtct cttcttgaaa
    13021 aaaacacaaa attttgctag aatgagtctt atgaaaacat tctatgatgt gcaacaattc
    13081 ctcaaacgat ttggtattat tgtttacatg ggaaaacgct tatatgatat tgaactgatg
    13141 aagttggaac tatctcggat ttacgatgca gggttgatgg ataaattaga ctatctagag
    13201 gcagaagcag ttcttcgcag agagcacaag gtagaattgg attatattga gaaaaatgga
    13261 gaaaagaact aatggttact tggattttgt gggcacttat actagcaatg ttggcgtgga
    13321 tgggctttaa ctatcttcgt attcgccgtg cggctaaaat tgtggacaat gaggagtttg
    13381 aagccttgat tcgtacgggt caattgattg atttgcgcga cccagcagaa ttccacagaa
    13441 aacatatcct tcgtgcacgc aatattcctt caagtcagtt gaaaactagt cttgcagccc
    13501 ttcgtaaaga taaacctgtc cttctctacg agaaccaacg tgcgcaacga gttacaaatg
    13561 cagctcttta cttgaaaaaa caaggttttt ctgagattta tatcctttct tatggcttgg
    13621 attcttggaa agggaaagtg aagactagct aaccttattt ccaaattagt cgaatgatag
    13681 gatgaagcta ttgttttata gtataattaa tttgtataaa atttaaagag ttcttagaac
    13741 atgaaagaag aaaaatccac tttttgtggg tatcttgtcg attattcttg gtttgttatt
    13801 tccaattgta ggcctgattt tgggtatcat aggattggtc ttggctattt cataccaaaa
    13861 agagtcacaa ttagactata aaatagaaaa gattcttaat atcttaggaa ttgtga
//
LOCUS       DAVLFB010000047        13609 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLFB010000047 DAVLFB010000000
VERSION     DAVLFB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.047"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(778..1647)
                     /locus_tag="VXD58_001243"
     CDS             complement(778..1647)
                     /locus_tag="VXD58_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV0675946.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1871..2392)
                     /locus_tag="VXD58_001244"
     CDS             complement(1871..2392)
                     /locus_tag="VXD58_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV0675947.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2350..3456)
                     /locus_tag="VXD58_001245"
     CDS             complement(2350..3456)
                     /locus_tag="VXD58_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV0675948.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3567..3821)
                     /locus_tag="VXD58_001246"
     CDS             complement(3567..3821)
                     /locus_tag="VXD58_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675949.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(4161..4694)
                     /locus_tag="VXD58_001247"
     CDS             complement(4161..4694)
                     /locus_tag="VXD58_001247"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0675950.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYKKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(4681..4896)
                     /locus_tag="VXD58_001248"
     CDS             complement(4681..4896)
                     /locus_tag="VXD58_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0675951.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(4863..5006)
                     /locus_tag="VXD58_001249"
     CDS             complement(4863..5006)
                     /locus_tag="VXD58_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV0675952.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            5240..6958
                     /locus_tag="VXD58_001250"
     CDS             5240..6958
                     /locus_tag="VXD58_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV0675953.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAEFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7069..7416
                     /locus_tag="VXD58_001251"
     CDS             7069..7416
                     /locus_tag="VXD58_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0675954.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7695..8531)
                     /locus_tag="VXD58_001252"
     CDS             complement(7695..8531)
                     /locus_tag="VXD58_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0675955.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8544..9173)
                     /locus_tag="VXD58_001253"
     CDS             complement(8544..9173)
                     /locus_tag="VXD58_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0675956.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLLVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9183..9824)
                     /locus_tag="VXD58_001254"
     CDS             complement(9183..9824)
                     /locus_tag="VXD58_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0675957.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9970..11199)
                     /locus_tag="VXD58_001255"
     CDS             complement(9970..11199)
                     /locus_tag="VXD58_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV0675958.1"
                     /translation="MNNSQQMLQALEEQDLTKAEYYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLMLGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11189..12355)
                     /locus_tag="VXD58_001256"
     CDS             complement(11189..12355)
                     /locus_tag="VXD58_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0675959.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVIFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12499..13512
                     /locus_tag="VXD58_001257"
     CDS             12499..13512
                     /locus_tag="VXD58_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV0675960.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNEFHVPEAVCIR
                     FAP"
BASE COUNT         4148 a         2920 c         2396 g         4145 t
ORIGIN      
        1 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
       61 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
      121 aaacaagaat agagcaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      181 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
      241 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
      301 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatac ccccctaatc
      361 gcttatttta tagcaaaatg aaatagaaac tgaacatttt gttgaggaag gtaaatcaat
      421 tcctcacaat gtttgagaaa gaagggagga ctattccaac ttcaacccac tataaagcag
      481 acttacaaga tagggaaccc atctccgata atatatagta gaatgaaaac tgcacaattt
      541 gattggagaa ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa
      601 ctgcaaccta ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc
      661 tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca
      721 gtctgatcct ctcttattta tagatggtgc tcactcaaat gttaaaggag ctatttctta
      781 gagataaagg aatttgaata cagctactgc cgcaattgct gctacgatag gtgctactac
      841 tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgattttgg
      901 aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt
      961 aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac
     1021 agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc
     1081 aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt cagcaccaaa
     1141 gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt
     1201 aatcaaacca ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga
     1261 aatagttgag aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg
     1321 gtatgttgcc acaactaagg cttggccaaa gatagctccc aagacttgcg cgataatata
     1381 aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg
     1441 gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc
     1501 ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac
     1561 gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc
     1621 atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt
     1681 ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa
     1741 ataggattca tagtttccca gttcaacgga cttgtaagcg tttttattct tcacgaaaga
     1801 cctcgttctc aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt
     1861 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agactagcta
     1921 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     1981 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     2041 cagtttttgg agtacctggg tctggagttg gtttatctgg tgttgataaa cggtcatacc
     2101 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     2161 tatctggatc tttgtacttg tcaatcttac cagatacaac ctcgtcccag tttcctgttg
     2221 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     2281 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     2341 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     2401 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     2461 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     2521 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     2581 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     2641 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     2701 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     2761 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     2821 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     2881 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     2941 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     3001 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     3061 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     3121 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     3181 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     3241 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     3301 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     3361 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     3421 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     3481 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     3541 ttgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     3601 gtcttcagta gtttatatgc tctgtcatca tctttagctt tagaagcagc tgagtttagc
     3661 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     3721 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     3781 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     3841 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     3901 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     3961 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     4021 tcaaaccttt cttatacaag tcttcattct aaacgatttc ggctacacgg tgaatggttt
     4081 gattgtatac cgtcttctct taaccagcag tatccttagt tttaatgact actcgatagg
     4141 ttcctttaga accgttatag tcatctagtt ttaaaaaagc attccaaaca ttcaaaaata
     4201 gttggaactt cccttaataa atccgtcact ttctttttca agatagccca agcttgctca
     4261 ataggattca aatccggtga ataaggtggt agaggtaaga aaaagtgttt atcttggatg
     4321 cagagttcat ccaaaatgtt cttgggatga aaactagcat tgcccatgac aataacatga
     4381 ggtgtcttca aagcaggtag gagttgcgtt ttgaaccact ccacaaagaa atcgcttgtc
     4441 atgcttttct tgtaaatcat gggagctata aactctccgt ctacttgtcc tgcaacaatt
     4501 gaagttcgct caaaacgacg tccgctaatc tttttataga ctttctcccc tctaggagcc
     4561 cgtgcataag gacgatagag gtagcggtcg attcctgttt catcaatata tacgattggt
     4621 aaatctttta ggttatccaa aatatcaaga aactcagcga ctttctctgg ctcttgttcc
     4681 ttaaagctcg tcatcttttt ttaaagtgac cttaatatac tttaaagctg cccatacgga
     4741 gggcagagca caatcaaaat gtgccgcaat ttcccgtaaa aaagcatctg gatgagcctc
     4801 tacaaaggct ttcaattcct ctaaaggaat ctttcggttt ttgacgactc gctttttcct
     4861 ctctatgtgt ccttgttcac atctttcttt tcccacgtga agagagttct gacgccaaca
     4921 ccaaaaaact tggctgcctc gacatggctg tgcccctctt tgatgtaatc taatgctcgc
     4981 tgtttaaaat cagtactata tgccatagct ttattataat atgcttgttt aaaactaagc
     5041 aactatatca atgttttgtt attagaagac ttactttttt tactgaaatc gaatttttcc
     5101 taacctcaac atcatataac cccaaaattc aatctgacct ggtttgaaga gatgttcgaa
     5161 gcagataagg ttttactaaa actaaaaaaa atgatataat caaactgatt aaatgtttta
     5221 aaaataaagg agaatttgaa tgtcttacca agaaaattac cagaaatggg ttgattttgt
     5281 ggagcttcct gactaccttc gtcaagattt ggaaaatatg gacgaaaaaa ctaaggaaga
     5341 tgccttctat acaaatcttg aatttggtac tgcaggtatg cgtggcttgg ttggtgctgg
     5401 tacaaaccgt atcaacatct acgttgttcg ccaagctact gaaggattgg ctcgtttgat
     5461 tgagtcaaaa ggtggaaacg agaaagaacg cggtgtagca attgcctacg atagccgtca
     5521 cttctcacct gagtttgcct ttgaatctgc ggcagttctt gctaaacacg gcatcaaatc
     5581 ttacgtattt gaaagccttc gtccgactcc agaactatca tttgcagttc gtcatctcaa
     5641 ctgtttcgca ggtatcatgg tcacagccag ccacaaccct gctccattta acggttacaa
     5701 ggtttacggt gaagacggtg gacaaatgcc tccacacgat gcggacgctt tgactactta
     5761 tatccgtgca atcgaaaacc catttgcagt tgaagttgct gatgtggaaa ctgaaaaagc
     5821 ttctggcttg attgaagtta tcggcgaagc tgttgacgta gaatacctta aagaggttaa
     5881 ggacgtaaac atcaacccag ccttgattga agaatttggt aaagacatga agattgtcta
     5941 cacaccactt catggtactg gtgaaatgtt ggctcgtcgt gctcttgccc aagcagaatt
     6001 tgactctgtt caagttgttg aagcgcaagc aactgctgac ccagacttct caactgtaac
     6061 atctccaaac ccagaaagcc aagcagcctt tgcccttgct gaagaacttg gtcgtcaagt
     6121 tggtgcagat gttcttgtcg caactgaccc agacgctgac cgtgttggtg ttgaagttct
     6181 tcaaaaagat ggtagctacc tcaacctttc aggtaaccaa atcggtgcta tcatggctaa
     6241 atacatcttg gaagctcaca aaaacgctgg aactcttcct gaaaatgccg ctctctgcaa
     6301 atctatcgtt tcaactgact tggtaacgaa gattgctgaa agctacggcg caactatgtt
     6361 caacgtcttg acaggtttca aatttatcgc tgaaaaaatc caagaattcg aagaaaaaca
     6421 caaccacact tacatgatgg gatttgaaga aagcttcggt tacttgatta aaccatttgt
     6481 acgtgataaa gatgccatcc aagccgttct tgtcgttgct gaacttgctg cctactaccg
     6541 ttctcgtggt ttgacacttg ctgacggtat cgaagaaatc tataaagaat atggctacta
     6601 cgcagaaaaa acaatctctg ttactctttc aggtgtcgat ggtgctgaac aaatcaaagc
     6661 gattatggct aaattccgca acaatgctcc aacagaatgg aacgcaacag ctatcactgt
     6721 cgtagaagac ttcaaggcac aaactgctac tgttgctgac ggtactgtta caaacttgac
     6781 aactcctcca agtgatgtgt tgaaatacac acttgctgac ggttcatgga ttgccgttcg
     6841 cccttcaggt acagaaccaa aaatcaagtt ctacattgca gttgtagggg aaaccaacga
     6901 agaatcacaa gctaagatta ctaacatcga agcagaaatc aatgcatttg taaaataaag
     6961 acctataaaa gatgagacca accaacctca tcttttttcg tatcaaatct aagcaatttt
     7021 agaaacttta tggcatacta gacttatcaa tgaaagcgag gtaatctcat ggaacaattt
     7081 ttagataaca tcaaagacct tgaagtcact acagttgtgc gtgcgcaaga agctcttgat
     7141 aaaaaagaaa ctgcaacctt ctttatcggt cgcaaaactt gcccttactg ccgtaaattt
     7201 gcaggtacat tgtcaggtgt cgtagctgaa accaaagctc acatttactt catcaatagt
     7261 gaagaaccaa gccaactcaa tgatttgcaa gcattccgct cacgttatgg aatcccaact
     7321 gtaccaggtt ttattcatat tacagatgga caaatcaacg tccgttgcga ctcttcaatg
     7381 tcagcacaag aaatcaaaga tttcgcagga ttgtaaaaaa tcagctaaca agtcttaaaa
     7441 tcacaaaaac gcataatgta aggtgcaaaa accttgatat tatgcgtttt attgttccta
     7501 aaacagttac atcagcattt cattacagaa cattttaatt ttggaagtca aatcaatttt
     7561 taacaatgtt ttagaaatca cagtgggcta ttccatattc aattatccag attcaatcgc
     7621 tattatcgtt ttcctagatt acaaaaaaaa ctacgttttg ctaatcaaaa cgtagtctca
     7681 gccactattt tatcttactg tccttctttt acttctttgg ttgctacatc ttctccaaac
     7741 catttttggc tgatttcttg gaacttgccg tccttgtaaa gactagaaaa agcttcattt
     7801 atcttcttaa ccaaggttgt atcttcctta cgggctccaa ccgcaaaagc ttctgtttct
     7861 agtccaactg taaagacatt ataatcgttt aaaacacctt ctgcttctaa ataatagttt
     7921 gcatagacac ggtcaatcaa tagaccatca attcgatcgt ttttcaaatc aatcaaggct
     7981 tcattaaagg tttggtattg attcgcttcc ttattagcga caatattctt caaaatttct
     8041 ggatttgctt caaagtccgc ataaccagat gaaccagctt gagctcctaa tgtctttcca
     8101 gtcatatcct ttgcagtcgt gataccagat gattttttcg taaccaatac ctgctcattc
     8161 ttcatatatg agttactgaa agccaccttt tcacggcgtt cgtctgtagc ggaatagcca
     8221 ttccaaatca gatcaatcgt tccttttgtc aattcagctt ctttcaaatc ccaatcaatc
     8281 ggttgccaat ttaccgtgat tccgtatttt tcaaaaacag ctgtagctaa atcaatatca
     8341 aatcctgcat aagaaccatc tttctgagca aatcccattg gaacaaaagt actatcaaat
     8401 ccaatagtaa tagacttgtt agactggtac tttgaccaat tatctccact agtttcgcta
     8461 gaatttttcc cacaagctac taagaacaaa gcagtcatca gactgactaa tacaagcatc
     8521 cattttttca tccatttttc ctcctattta ggttctactt tcaataatac atctgcgata
     8581 ttttcagcaa actgcaaatc atgggtaacc acaatctggg tcatcccaag ttccctattt
     8641 tgcaagatta gcttctccac ttccaaacgt aattctggat ccagggcaga agttggttca
     8701 tcgtagccaa tgatttctgg gtcaatcatc atagcacgcg ccaaagccac ccgctgcttt
     8761 tgcccaccag atagtgagaa aggataggcc tctgcgtgtc ctcctagtcc taactgttcc
     8821 aagagtccac tcgccttctt ctcagcctct tcctgcttca ttcccatggt cttcacaggc
     8881 gataaagtca aattttccag aactaataga tgaggaaata gttgaaaatc ttggaagaca
     8941 aatcctagta ggttgcgctt ctgcaattca tccagctcta aaggttgtcc attataaaag
     9001 atttgccctg aatcaatggt ttcaagaccc gcaagcatac gtaaaagagt cgtcttacct
     9061 ccaccagaag gtccaacgat agccaggatt tgcttttcag gaatacttag actgaaatta
     9121 gacaggattt gtttgtctcc aaagacttta ttgatatttc gtaattctaa catggcagcc
     9181 tcctatctat aataactata cttcttctca acttttttgg aaataattgt cacaatccca
     9241 atcaaaatca aataaatggc tcctgccaag aacataggaa ctagactagc atcgcggtta
     9301 gcagctgttc gactagccaa gataaggtct gaaattccga gagcatagac caaagaagta
     9361 tccttgacca aactcataac ttcattaaag acactaggaa gaacgatctt ggtcacttgg
     9421 ggcaagataa tatagcgcac tctgtcaaaa gggctaaact tcaagacctt ggcggcctca
     9481 tactgtcctc ttggaatagt gtcaattccc ccacggaaaa tttctgcaaa gtaagctgca
     9541 tagttgagaa caaaggcaat aatagctgca ggaaggcggt ctaaacgaat cccaatactt
     9601 gggagcacat aatagataaa aattagttgc aagagtaagg gggttcctcg cataacccag
     9661 atgtaaatgt taatcagata atggaggggc ttccaatgga cttgcaaggc aaaagcaatc
     9721 aaaacgccca agggaatcga aaatagcaag accagtgcaa agacctgtac agtcgtgctc
     9781 gcaccattca gtaaactcgg taatatctca aataaataag acatactgca cctcctaaaa
     9841 gtaattgttc ctattatagc atgaataaac aagattacca agtatttttg taaaaagatt
     9901 ctaccatata caaataaaaa ccgtagctat caaaagagat aaactacagt tctatatgtt
     9961 taaacattct tacaatctct caaacagttc ttgcatttgc acatcatctg gaaccagttt
    10021 taagtaagtg tgagcatgga cttttgcttc ttcaaaatgt cccaattcac gcaagagata
    10081 gatatagtgt tccagaaatt ctggattgtc cttcaaatct cctgtcaact cttgataatg
    10141 ctcataagca gtatccaaat cgtccatttc ttgataagaa cgagcaatca tccacttggt
    10201 caaaagattt tctggctcct cactctgcaa gtctagaata tcctcataac gctcctgctc
    10261 cagataaata gtggctaaac gaagcaagat ttcttctgta tcctcagcgt cttcttttgc
    10321 agcaagaaga taattttctg caccactagc atcatgcaat tcataagaaa attgtgaagc
    10381 agctagcaag aggcgagttt caaagggatt tttctctaat ccttgcttag cgatacgcag
    10441 ggcttcttga acttgatgtt ccttatgtaa agcctgactg tacccatact catagccttc
    10501 aaagtcagga gaaatggtat caagctgctt aaagtagagg gtggcttttt gatattcttc
    10561 ttgatcaaaa taaagactgg ccaactcaaa agctgttaag tcatcgtatt ctaactccag
    10621 ggctttttct aaaaactcag tagccgtttc aaatttccct aactgagcat aggcaaagcc
    10681 aattcgttga taggtggaaa tgcccgtttg ctcataaatc gagcgattat ctaactgggc
    10741 ataggcttga atagccgctt ggtaattttc caactcacta tccaactctg ccaaacccaa
    10801 catcaagaga gaatcctctg agtaggtcaa ggcctccaat aatttctcac gtgccacatc
    10861 tgtcaaacct tccagctggt aaaggtctgc cttcaaaacc aaagacgaga cataccagtc
    10921 actgtcagct tggatttcct caagataggt aaaggcttct tctatttgac catcctcgct
    10981 agcaattgca gctagattaa gatgaacctc tggaaaatcc tctacaattt tcaggtaaat
    11041 ttccttggcc tgaggataga aaccaatccc ttcaagataa gttgccaact catacagaag
    11101 atcacttgaa tcattttcta aagctttggc gaaataatac tcagccttag ttaaatcttg
    11161 ctcctccaaa gcctgtaaca tctgttgact attgttcact cttgacttcc tcaccctcta
    11221 attcatatag accactgact accttatacc attcgaaaat ggctgaaatg acaaccttag
    11281 cagaggcata aaccggaata ccaagtaaaa ctccccagat accaaacata gatcctgaag
    11341 ttaacaaaac aaagagaaca ttaatagggt ggatgtttaa ttgacttccc aaaatcaatg
    11401 gagagacaaa acggccttca atagtttgtt ctacgataaa gacaatcact actttcaaaa
    11461 gcatgactgg accagcaatc aaacccaata ctagagcagg aagcatggct agaaagctac
    11521 caagataagg gaccagattt aaaataccag cagtaacccc cagcgtaacc gcatagcgta
    11581 gaccaataat cttgaagaag atgataaata ttactgctac aataatagcc actgtcactt
    11641 gccctcgaac atagttggac aactgttgat tcacatctga taaaacttgt ccaacaggtt
    11701 ccttcaattt tcttggaatg aattgggtca aatagttacg caagcctttc ccatcacgca
    11761 agagataaaa gagcatgaaa ggaacgataa tcaaggcaac aatcacttga gaagctccac
    11821 taataaaggc actcacccag ttgactgcct gagatgaaac cttacttgcc aaaactgtag
    11881 cctggctaga aaaattggtc aaaacttgct ctaattgagg tctgaaatca tctggcaggt
    11941 gctgggctac caatccatta acaatcctat ctatatcttc taagtaaaca ggaacgtttc
    12001 ttgcaaaggt caaaacctga cgttgcagat ttggaatggc gactgccaag ccccaaatga
    12061 taaagagagc gatgataaca aagacaatag tgatagctat aacacgatta accttatgct
    12121 tctccatcca atcaacaata ggattcaaca aataatataa caaaccagac aaaatgactg
    12181 gcaacatcac aactgctaaa aaatctaaaa caggtgaaaa tagaaaacta atcttactta
    12241 aaataaaaag attcaatccc aataataagg ttactaaaaa taccgtaatt gccttgttat
    12301 ctaaaaacca cttgaaaaac caagataggc taaaatgttt ctctttttgc tccataaata
    12361 cttcctttct attgttctct cattatacca tttttgaaac aaaataactc ttttttaaag
    12421 tgcttacatc taattgaatg tctttaattt gcttgttttt gtgatataat agtcttatct
    12481 ttacacagag gtatagtaat gaaagaaact gtttattttg gaacttatac acgtcgtact
    12541 tctcaaggga tttacaaggc agactttgat acagaaactg gtcagctttc aaatctagaa
    12601 ctttttgcag ctgagccaag tccaacctac cttgcctttg accagcacca acatttatac
    12661 actgttggta gccaagacga taagggggga attgcagcct atcaaactga cgggactgtg
    12721 ttaaatcatg ttgttgaaga aggagctccc cactgttatg ttgctgtcga tgaaaagcgt
    12781 gatttggttt acgcagccaa ctatcacaag ggacaagtcc ttgtttataa acgccaggaa
    12841 gatggtagtc ttctacttag tgatatggat caacacagtg gccaaggtcc acatgaaaat
    12901 caagcttccc cccatgttca ctatacagat ttaacacctg accactatct agtgacctgc
    12961 gacttgggaa ctgaccaagt catcacctat gacctcgatc aagaaggaaa attatctaag
    13021 ctctatacct atcacagcaa gccaggagca ggctcacgcc atatcatttt ccataaccac
    13081 tataaaatcg cttatctcat ttgtgaactc aatagtacta tcgaagtttt aatctacgat
    13141 ggcgttggcg aatttgtacg tatgcaggtt atttcaactt taccagaagc ttacgaaggc
    13201 tttaatggaa ccgctgctat tcatctctct aaagacggta aatacctcta cgcttctaac
    13261 cgtggccatg attctatcgc agtatatacc atccttgcgg acggtagctt agagttatta
    13321 gaaatcgttc caacgcatgg tcagactcca cgtgattttg atttgacacc cgaccaaaaa
    13381 tttctcattg ttgtccatca agactctgac aatgcaactg tctttaaacg taattgtgac
    13441 aatggtcgtc tagcagaact ctccaacgaa ttccatgttc ccgaagcagt ctgcatccgt
    13501 tttgctcctt aaaacaccat aaaaaatggc tctataatat ttgtagtggg taaatcccct
    13561 atggatatta tggagcctat ttttgtgtag aaaaaaagtc ccataagat
//
LOCUS       DAVLFB010000048        13359 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLFB010000048 DAVLFB010000000
VERSION     DAVLFB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.048"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(179..943)
                     /locus_tag="VXD58_001258"
     CDS             complement(179..943)
                     /locus_tag="VXD58_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675961.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1039..1442)
                     /locus_tag="VXD58_001259"
                     /pseudo
     CDS             complement(1039..1442)
                     /locus_tag="VXD58_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1622..2254)
                     /locus_tag="VXD58_001260"
     CDS             complement(1622..2254)
                     /locus_tag="VXD58_001260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV0675962.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2288..2989)
                     /gene="pyrF"
                     /locus_tag="VXD58_001261"
     CDS             complement(2288..2989)
                     /gene="pyrF"
                     /locus_tag="VXD58_001261"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV0675963.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            complement(3222..4421)
                     /gene="divIB"
                     /locus_tag="VXD58_001262"
     CDS             complement(3222..4421)
                     /gene="divIB"
                     /locus_tag="VXD58_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV0675964.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(4431..5489)
                     /locus_tag="VXD58_001263"
     CDS             complement(4431..5489)
                     /locus_tag="VXD58_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV0675965.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5493..6845)
                     /locus_tag="VXD58_001264"
     CDS             complement(5493..6845)
                     /locus_tag="VXD58_001264"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV0675966.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(6949..7590)
                     /locus_tag="VXD58_001265"
     CDS             complement(6949..7590)
                     /locus_tag="VXD58_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0675967.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7592..9613)
                     /locus_tag="VXD58_001266"
                     /pseudo
     CDS             complement(7592..9613)
                     /locus_tag="VXD58_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(9696..9995)
                     /locus_tag="VXD58_001267"
                     /pseudo
     CDS             complement(9696..9995)
                     /locus_tag="VXD58_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            complement(10360..10614)
                     /locus_tag="VXD58_001268"
     CDS             complement(10360..10614)
                     /locus_tag="VXD58_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV0675968.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            complement(10634..12475)
                     /gene="typA"
                     /locus_tag="VXD58_001269"
     CDS             complement(10634..12475)
                     /gene="typA"
                     /locus_tag="VXD58_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV0675969.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQETTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            12637..>13359
                     /locus_tag="VXD58_001270"
     CDS             12637..>13359
                     /locus_tag="VXD58_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV0675970.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
BASE COUNT         4015 a         2932 c         2398 g         4014 t
ORIGIN      
        1 actccagtcg tcatatcttg aagtaaaact aagatccatt ctaatacatt tggatggatt
       61 gaatattggc gacagcgcaa taaatatact gtactagata aaacacagga tagcagtaat
      121 ataaaataaa ccaatactga taaaaaatct tttttgtaaa attgaacaaa ttgtttcatt
      181 atacgtagtc ctctgaatgt agaaaaaatg taccataaac aaccaaacaa ctaacaaata
      241 aaataaaagc aagatgccca ctaactaagg aaagactgat atctttctga tatcccaaag
      301 ctaatgttgt cacaggttct aagtaagata gccctaaaat agcccaaaaa ataccaccaa
      361 ccatcatata ggcaactggg atgaaaatag ctcctatttt tttcttcact agcaaagcac
      421 tagctagtcc aaaaatagag aacacagcgc cccaaactcc ataccagaga gtcgttacaa
      481 gactatagag aaactgattg gaatcaaata attcttttaa ggcaccacta taatctccaa
      541 tataaatttc ctgataagga gtcactaaaa gattaattcc taataataat aaatagggga
      601 gaaaaaagac tagaaaagaa gaaataatta cagtactacc cacaatagcc agatacttct
      661 ttttagaaat tcgacacaat tgtgctgtta gaaaatgact ctcagcatcc tctattatct
      721 gactagaata gggcagtgta cagataagtg cagctactag gctaatcggt gaaaatcctc
      781 gaatagaaga agcggcaaaa aaaatcgata aaccttcaat tttataaata ccattgaaag
      841 caaggaaatt tcctaaactc atgcaaagaa gggtcaaaaa taaagacata taaaatcgag
      901 gtgattgaac gactcgtaca agattacaaa tgaaaaattc catccttact cctccttata
      961 ataaaaatag ggtgtagcat tcttttttca tgctacaccc acaatcaacc atctctaagg
     1021 cttactctga caagtaagtt aataagaatc tggattccaa gaacctgaag tatgaattct
     1081 tacatgattt ccaaattgtg gcgccatagc taatctagta ccagaaccaa tataattgtc
     1141 accacctcca ttatagtaca tgacaatcct agagccagac cccaatgaat ataccggggt
     1201 aatatctgac ccactatagg cgctacgaat agaggtattt aaccttttac cgccaccagt
     1261 gctgtcatta ttattaattc cagcagaggc gttttctttc tcaagctcac tagttgtatc
     1321 cgaaccccaa cgcggcaacg ttaaatcata atctccaatc tgtctagcta aaactgtaag
     1381 gagaaacaaa ctagatgcaa ttgtaactac gcaaaaaagg aatatatttt tcttcttttc
     1441 atgacatcgt cctcctaaga agtgataaac aagatagttg attgatcaat ttgatacata
     1501 ttacattttc attatatcaa caaaagaaag cgttgtcaat cattttattt aaaatattgt
     1561 catattttaa ataaaatgat aaaagaggct cggtagcctc atctctcttt atcttactga
     1621 cctaaccttc ttgccaattt tcttggtctt ctttaaagcg ttttagaaga tcaaggccct
     1681 ctggcgtgat gtaaccttct tcttgggcta gatggataag ctcgctatag tttgaaagcg
     1741 tcacaagttt aacaccagca tctgcaaagt tcttatctgc ttttggcaat tggtagctga
     1801 aaatcgctac aactccaagc acatctgctc cttctcgctt ggctgctgct acagcttcaa
     1861 gaactgaacc acccgttgaa ataaggtctt caaccactac cattttttga ccttgagcta
     1921 cgcgaccttc gatttgatta ccagctccgt ggtcttttgg tttgctacgg atgtaggcaa
     1981 aaggcaagtc catcttatca gcaataatgg ctccgtgtgg aatccctgct gttgcagttc
     2041 ctgcaatcac ttctacttca ggaaaggctt ctttgatagc ttccacaaaa ccattttcaa
     2101 ttagggtacg agtttctgga taggctagtg tcacacgatt atcagtgtaa atcggtgact
     2161 tgataccaga tgcccaagtg aagggttcct ctggtttgag gtaaacggct tggattttca
     2221 agaggtggct agcgatatct ttagcaagtg tcatggtatt ctccttttat ttttctaatc
     2281 tagttcttta attccagtcc tgtgtccatt catccttgat ggtatgataa gctgcaacag
     2341 gatcctcagc ttgggtaatg ggacgtccca ctacgatata gtcactgccg atttgatagg
     2401 catcagcagg tgtcatgact cgtttttgat ctccaactgc agcaccagct ggacgaatcc
     2461 ctggtgtcag acagataaaa tctggattgg tagcctgctt gatgacttgt acttcctgag
     2521 ccgagcaaac aacaccatcc aagccagctt cagctgtctt cttggcatag tgaatcacag
     2581 actcttgcag actggtttgg atattttgaa actcctgcat ctgagcttct gacgttgatg
     2641 tgagctgagt tacagcgatc aatttagctt gactcccaag gccttcacgc gccgccttca
     2701 tcatctctac accaccagcc gcatggacat tagtcatatc gacaccaagc tgagacaaga
     2761 ccttcatggc tgacttgact gtattaggaa tgtcatgaag tttgagatcc aaaaagacac
     2821 tatgacccaa accttttaag taggacacaa tctcaggccc cgctgcgtaa taaagctcca
     2881 tccctacctt gagataaagg ctttcttctg ctgggaaaag agctaaaaat tccttgaccg
     2941 cctcaaaact aggaaaatca agagcaatga ctggacgatg ttctcgcata ggtttctcct
     3001 tttaccttta ctagtgagag agtctggcca acagaagcca cgaaaaaagg aacctgccaa
     3061 cagcaaggtt ccacgaaaaa tgggtagtct ccaccctttc accgtctaac cttagctgcc
     3121 tctctggact gctttaaagg ttttttacta ttctattatt tctactagca cttgtcaagt
     3181 aaaaacatgg tcactaaaga actataagag aaaaggtaaa cctagcgacg cgatgagcgc
     3241 tgggtcgttt ggtttcgatt gctctcttcc tcttgttttt tctgttcttc ttcttgtttt
     3301 ttctcagctt ccttagcctc ttgtttggct ttttcctcag cttccataat taatttatcc
     3361 gccacagtgt aactgtaaat tccagcttcc atgtcaatca cactcggttc tgacaattgt
     3421 ggcttaatct tactataata aggcagtttc ttgctcattt cagaaagcgg aaccaagact
     3481 tcgtccaaat cattcatagt caatcgaatt aaatcggatg tcaccttgct tggggctaat
     3541 tccacctttt ggatagccgc cttgagttct gggctaattt gagcaagttc tgagacaaaa
     3601 accttgattt gttcactatc attaaagaga actgataaat aagtttctgg taaactgttc
     3661 aggctcacag aactagtctc aagctgacca ctggaaagaa taggataatg attttcacca
     3721 gaaatatagt aggccacaat atcatattcc ttgaccttaa tagtgaactt agttggaaat
     3781 tgatagacaa gttgagctga ttcaacccaa tagttagact taatctgctt ttcatatttt
     3841 gccttgtcta gcagaaggtt aatcgtataa tccgaatcct gaatgcctga agcctgtcga
     3901 atatcatcag ctgtagtttg caccgttccc tcaacacgaa tatctttcat ggtcgcataa
     3961 ggactgagca agtaggcaga gacaatcaat aaaagcagac ttggaaataa aatcgtgaag
     4021 gctcgcaaga tatggatacc aggaatcttt gctttggctg gtttttcctt tgtagccttt
     4081 ttagcaagct ttttatcctg ttcctccttc tctttagact ctggttcttc tttctcttct
     4141 ttctctttgt catcctctga ggatgctact ttttcttcag actcttcctt agctgattct
     4201 gaatcttcct ggtctgtttc actctcctgg tcctgtttgt cctctgactt ctcagattct
     4261 tctcccatcc gagcttgtct ttccttttcc ttctcctcag ctagagccgc ttcttcttca
     4321 gccttctttt ttagatattc ttggtttcgt ttctgccatt ctgataactc tttcaattct
     4381 tcgaggattt ctttgtcctc atttttctta tcttttgaca tttactttcc ttatgataaa
     4441 tcttttttca acaattgata aaaatctgct agagatttca attccttaga agctttcatc
     4501 ttagcttggt aatcttcctt gtgacttagt aagtgagaaa gcttctcttc caaactatcc
     4561 aaggtcaaat cgctttcttg aaggtcttct gcatagcctt tttttacaaa gtaagctgca
     4621 ttttcaatct ggtcaccacg actagcttca cgaccaagcg gcacaatgac atgcaatttt
     4681 gctatcgcca agagctcaaa aatcgtattg gcaccacctc gtgtcacaac aatatcagcc
     4741 aattccaaca agggttgata gagatcggtc acatagtcaa cacgaaaaag attttggctc
     4801 aactcattca ggctagaatc tccagttaga ttgataatat tgtagcgctc tgttagttct
     4861 ttcttatggt ctgtcaccaa ttggttaaag acacgagcac ctgcagaacc gccaacaaac
     4921 aatacagtcg gcaatttatg attaaagtgg gtttgaatat ccaccaattc atctggttct
     4981 ggattttttt gatctgaaac cttggtcact gctcccacat gctcaacctt agacaaactc
     5041 gaagcttgct caaaggttga atacatctta gtcgcaaatt tataggcgat tttattggcc
     5101 aagcccatag acaggtcaga ttcgtgaata aagacaggca ctcctgacac acgcgctgcg
     5161 ataacaggcg gtactgagac aaagcccccc tttgaaaaaa gggtctgtgg acgaagtcgc
     5221 aacatgataa agagcgattg gacgattccc cagccaactt tgaagacgtc cagcatattt
     5281 tgccaagaga aatagcgacg caatttccca gtcgcaatgg agtggaaagt gacatccaaa
     5341 cctgacttaa ggatttcttg gtgttcgata ccacgcttgt ccccgatata gtggacttcc
     5401 caaccatctt cgatgaactt gggcattaac aaaagattga gggtaacgtg tccaaccgtc
     5461 cccccacctg taaagacaat ttttttcata ttttattctt ttaactccgc tactgtgtcg
     5521 ataaagaggt cgccacgtac ttcaaagtta gcatacatat cccagctggc attggcagga
     5581 ctaagaagaa ccacatctcc ttgagtcgca agctcatagg ccttgcgggt cgcatctgca
     5641 atatctgtcg cctccacata agcgacacca gccttgtctg ctgcccgttt gacacgttct
     5701 gcagattgac ccaggatgac catcttcttg agtccagtaa tgtctggcac caattcgtca
     5761 aactcattgc cacggtccaa accacctgca atcaagacga ccttgctgtt gtcaaatcct
     5821 gataaggctt tttgagtagc caagatatta gttgatttac tgtcgttata gaatttaaca
     5881 cccttgatgt catccacaaa ctggagacgg tgtttgacac caccgaaggc tgaaagagtt
     5941 tccttgatgg tttgattgtc cacatcacga agcttggcta cagcaatagt cgcaagggca
     6001 ttttccacat tgtggctacc tggaacaccg atttcattcg ctgccatgac tacttcacca
     6061 cggaagtaga gttgaccatc ttccagataa gctccatcaa ccttttcaag tgttgaaaat
     6121 ggtacaacag tggcttctgt cttggaagcc aagtctttta ccaagtcttg attaaagttc
     6181 aagacaagga aatcagctgc tgtcatcttg ttctggatat tccacttggc tgctacatat
     6241 tcctcaaata atccatgata atcgatatga gttggcatga ggttggtaat aaccgcaatc
     6301 tctggatgga attcttgaac acccatgagt tggaaagaag aaagttccat aacaagcgtg
     6361 tccttatctg atgctatttg agcaacctga ctggcaggat agccgatatt ccctgataaa
     6421 agaccatgtt gcccagcagc agtcaaaact tccccaatca tagtcgttgt ggttgtctta
     6481 ccgttcgatc ctgtgatacc aataatcggt gcttctgaaa tcaaataagc caattccacc
     6541 tcagtcaaga ctggaattcc cttggccaaa gccttttcaa tcatgggatt gctgtagggg
     6601 atacctggat tttttaccat gagggcaaac tcttcatcca agagttccaa aggatggcca
     6661 cctgtaatga ccttgatccc ttcttccagc aaacattggg cagctggatt gtcctcgaat
     6721 ggtttcccat catttactgt cacaatggca cctaacttgt ccaacaaacg agccgcagat
     6781 tcaccagact tggccaaacc taaaacaaga actttcttat ttttaaattg atctattact
     6841 ttcatgtctc gaactccatt tctactccta ctattttacc atttttatgg aaataaaaaa
     6901 gccacaaagt gtgtttgtga ctctttcttc taactgaatc ttaccatatc atctatgtga
     6961 taaatcggta actcgaatga cctgatccac ttgctcccaa atcagaggat tgtgggtcgc
     7021 aataataatg gtccgattcg gattttttaa agattctagg atggaaagta attcctcaga
     7081 atttttgggg tctaaggaag cggttggttc atctgcaaga atcaaaggcg gatcctttaa
     7141 aattatcttc gctagtgcaa cacgttgtgc ttctcctcct gataactcaa atataggttg
     7201 cttcaaatcc aaataagaga ggtttacaca gtttagagct tgtttcatca aagagatttt
     7261 ctctttttcc ttcaactttt taccaactaa acccagattg agattctctt tgacggtttg
     7321 gctttcaatt aagccaaaat cttgaaataa gtatcctaag taatctctaa agaaaacaga
     7381 aggcttgatg tccttaagag aagtgccatc atagatgatt tgccctttgt catatggctc
     7441 caatcgtcca atcatattca agagtgttgt cttaccacag ccacttgtac cgattaaggc
     7501 ataaattttc ccaccttcaa aatgaagatt cgtatctgaa aatagctgac ggcttccaaa
     7561 ttttttagat atattcttta gttcaatcat cctattttcc tttcataatt gtcatagaaa
     7621 cacgagattc tttctgcgct tgacggtaaa gcgtcaaaac tgcactagct agaaagacca
     7681 ataaagtgag caagccaatc accaagtctc gactgcttaa aataaagaga ctagcaccaa
     7741 atacaaaact ggcaaattgg ctaaccatat actgagcatg tgtttcaaaa aatcgtaaac
     7801 ctgaaattcg tttaatcaag atatctcggc ggaattgctc gaaatataga agattgacag
     7861 aataaaatag taataaggaa ctagctatcc ccacaatagc tcctaagatt aaagttgctg
     7921 tttcagtttg aacttcatta taacgagtta gataaacact tcttccttct ttaagatagg
     7981 atacttgctc ataaattcca gctttcttca agagttctag cccactctca tatcctttga
     8041 taaagagttg ttttccagca ttgatagacc aactagataa ggatataaaa ctatcacctg
     8101 tagaagtcgg cgtgaatacc actaaaatcg gatcagtcaa atactgagta gatacgggat
     8161 tctcaccgtt attataaaca aaccgctttt ctcccattga aagataacta acgtgcgctt
     8221 tcatctcata atccaaagga gcacttgcct cctcaccaga ttttccataa taactcaatc
     8281 tttcttcaaa aactttctta agttctgctt ctcgagagcg caaatgttct gggagcaaga
     8341 ggataaactc acctttttgg agatgggcta acttctgttt ggtctcagca tctaccacga
     8401 ccttttcctt gtccaaataa ctgggactaa catagagcgt attagcatct ggactatagg
     8461 tatccagtgt ctctccctgt tcattttttc cttgtggatt ggcaaaatgg agcagattat
     8521 cctttacata aagagcttgt tcctcttcga ttgcttcctt ggcaaaggca taccacttgc
     8581 tctgattttc tgtatctttt cctctatcac ctaagccaaa agaaatttga taatagtctg
     8641 ctctatcctg ccatgcttgt tttgaaattt caagttcttt caatcgttgg taagacgtca
     8701 aacctgtctt aacagcgtat cctactgtaa agacagcaac caactgacac aatagggtta
     8761 aaatcatcag gcgtttaagg ggtaatctcc ccttaataac gggaactaat gctttgtaac
     8821 tcaaactcat tagataaaga agcattagta aaattgaaat cgccaataaa aacaacagat
     8881 agaaactaat cccaaaacca taggtggcta acaagatagg ataaaacaaa ccttgactaa
     8941 aaagaacgac tcccccacct aggaaggaaa ggagggctga tagaaggagc tatttgatat
     9001 cagtagataa agaatgcccc atgatggata agagagtctg accagaaaag agttttatac
     9061 ctgctgctct catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa
     9121 atattgctaa actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg
     9181 gcggtatctt tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct
     9241 tttctttcgt caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac
     9301 gactttcttg gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat
     9361 aagtaaagtg agtcgtccca tccttactcg gctctacaat tcttctagct attaaactct
     9421 gttctgagtt tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag
     9481 tatctttttt gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg
     9541 cacgtaagac aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga
     9601 atagtttctt catagtagac tccttggtag actccttgta aacaaaattc cccctgtaat
     9661 ttcttacaag gggaacgatt taaatcaatg aacgattagt cataatcaca gtaaaatgct
     9721 acttgttctc cccatttagt ccaaatccat gcataggagg tttttccagc aggagcttct
     9781 cctttatcag aatgaccatc ccatctgctc acgatggatg aataatgata ttttttacca
     9841 tgatagtaat ttgaaaaagc ctaaccacct cctgaacctt ctccatatgt ccatactcct
     9901 ccatctggat attatacagc agctgatgca gctcccaata atgtaaaact tgaaataaga
     9961 gctagagcaa gtaatctatg ttttttcgtt ttcattttat tttttctttc aaaaaaatca
    10021 caccttgagc aacaatgcaa caaaataaat cctcctctct cttttattga aaccgctttc
    10081 ttatgtgata agaataacat ttttattatt tgttgtcaag gaaaaaatcg aattttttag
    10141 atattttact atattacctc tatgaataat attatatagt agttttattt caaaataata
    10201 tgcaaccagt actaaccaaa tataaaatag attccattaa cgaattttat tcaagttttt
    10261 cccattcata ttatccaaac aaaagagatg gtgataactc aaaaataaat tgcaatcttg
    10321 taaaattcct agcaaaaaag agagccaaaa ctctcttttt tatcttcttt tacttttttt
    10381 gactggcatg agtgtaatgt ctctaacact aaagtaagct aggagcaaca tggctattgc
    10441 taggaatatt tctgttggta attgaaaaat tttcagaaaa gatagaacca ataaaatcaa
    10501 gagtgccact aaaatacata ccatagcgac gatattgact gtccctttaa tgctttctgg
    10561 tgtcgcaaat acatagagta ggagcagtaa aattcctagg actaaataga ccatctttct
    10621 ctctttctag ctcttattca gctgattttt tcttcttgtt agctttctca cgctctgctt
    10681 tgttaaggat ttgtttacgc aaacggatag actcaggcgt tacttccatg tactcatcgt
    10741 cgttcaagaa ctcaagagac tcttcaagtg tcaagatacg aggcgtcttg ataacagctg
    10801 tttggtcctt agtagctgaa cgaacgttgg tcatttgttt tgccttcgtg atgttaactg
    10861 tcaagtcatt ttcacgagag ttttcaccga tgatcattcc ttcataaacc tcagtacctg
    10921 ggttgacaaa gatcgtacca cgttcttcga tagacatgat tgagtaagtt gtagccttac
    10981 cagcatcgat agaaacaagg gcaccacggt gacgtccacc aatttcccct ggaatcaatg
    11041 gcaagtattg gtcgaaggta tggttcatga taccgtaacc acgagtcatt gataagaact
    11101 cagttgagta tccaatcaaa ccacgcgctg gaacaaggaa gaccaaacga gtttgaccat
    11161 taccagttga aatcatatcc aacatttcac ctttacgttc agaaaggctt tggataacag
    11221 acccttggta ttcttctgga gtgtcgattt gtacacgttc aaatggttca catttaacac
    11281 cgtcgatttc ttttacgata acttctggac gagatacttg aagttcatag ccctcacgac
    11341 gcattgtttc gataaggatt gacaagtgca attctccacg tcctgaaaca gtccatttat
    11401 ctggtgaatc agttgggtca acacgaaggg aaacgtctgt ttgcaattct gcctgcaagc
    11461 gttcttccac cttacgagaa gttacccatt taccttcttt accagcaaat ggtgagttgt
    11521 tgaccaagaa agtcatttga agagttggct catcgatgtg taggattgga agagcttcta
    11581 ctgcatctgt cggagtgatg gtttcaccga caaagatgtc ttccatacct gaaacggcaa
    11641 tcaagtcacc cgctttggct tcttggattt cacgacgttc caaaccaaag aaaccgaaga
    11701 gttttgtaac acggaagttt ttagttgtac cgtcaagttt agaaagggta acttggtccc
    11761 caaccttaac tgtaccacgg aagacacgac cgataccgat acgtccaacg aagtcattgt
    11821 agtccaaaag tgacacttgg aactgcaaag gctcatctga gttatctact ggagctggga
    11881 tatggtcgat aatcgtgtca aagattggtg ccatagtcgt ttcttggtca gctggatcat
    11941 ctgacaatga agaagttccg ttgatcgctg aagcataaac cactgggaaa tcaagctgat
    12001 cgtcatctgc accaagctcg atgaaaagtt ccaagacttc atccactact tctgctggac
    12061 gagctgatgg cttatcgatt ttgttaacaa ccacgattgg gacaaggtct tgttccaagg
    12121 cttttttcaa tacgaaacga gtttgtggca tggttccttc ataggcatct acgaccaaga
    12181 caacaccgtc aaccattttc atgatacgct caacttctcc accgaagtcc gcgtgtcctg
    12241 gtgtgtccat aatgttgata cgagttccgt tgtaagcaac ggcagtattt ttagcaagga
    12301 tggtgattcc acgctctttt tcgatatcgt ttgagtccat agcacgctct gccaattcag
    12361 tacgtgcatc aagcgtttct gattgtttca ataattcgtc aaccagggtt gttttaccgt
    12421 ggtcaacgtg ggcgataatc gcaatgttac ggatatcttc tcttaatttt gtcatgattt
    12481 cctctataat attcaaaatt tattttctaa ctgaacgatt ataccataat ttcaaataaa
    12541 taacataact caagcaagtg taaatgtttt cactccgctt ttcttttcac gtcaagcctt
    12601 ttcaaagcaa gcgacttatg ataagatagg cacagtatgc gtttagataa tttattagct
    12661 caagaaaaaa tcagccgaaa ggccatgaaa caagccttac tcaaagggga cattctagtc
    12721 gatggttgcc cagcccgctc cctagctcaa aatatcgata caggactaca agaactcctt
    12781 tttcaggatc gaatcattca aggctatgaa cacacctatc ttatgcttca taaacctgct
    12841 ggtgtcgtta cagccaacaa agacaaggaa cttccgaccg tcatggactt gcttccatct
    12901 gacatccagt ctgacaagct ctatgccgtc ggccgactgg accgagatac aacaggactc
    12961 cttctcttga ccgataacgg tcccttgggc ttccaactcc tccatcccca atatcatgtc
    13021 gataagactt atcaagttga ggttaatgga cttctaacac ctgaccatat ccaagccttt
    13081 caaaagggaa ttgtcttttt agatggtact gtctgtaaac ccgcaaaact agagattcta
    13141 tctgcaagtc cctccatcag tcaagcctct atcaccattt cagaaggaaa atttcatcaa
    13201 atcaagaaaa tgttcctctc ggttggtgtt aaggtgacta gcctcaagag aatccaattt
    13261 ggggacttca cattgaaccc agatttagca gaaggtaact accgcccttt gaaccaaaaa
    13321 gagttacaaa tcattaaaaa ctatttagag atgagtcga
//
LOCUS       DAVLFB010000049        13228 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLFB010000049 DAVLFB010000000
VERSION     DAVLFB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.049"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            33..155
                     /locus_tag="VXD58_001271"
     CDS             33..155
                     /locus_tag="VXD58_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV0675971.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            177..1217
                     /locus_tag="VXD58_001272"
     CDS             177..1217
                     /locus_tag="VXD58_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV0675972.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWMASGKFPQFVDEGSSCHNLSYTRDVGAVAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDHLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            1388..2662
                     /locus_tag="VXD58_001273"
     CDS             1388..2662
                     /locus_tag="VXD58_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV0675973.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELCLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2672..3550
                     /gene="truB"
                     /locus_tag="VXD58_001274"
     CDS             2672..3550
                     /gene="truB"
                     /locus_tag="VXD58_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV0675974.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3791..4093
                     /locus_tag="VXD58_001275"
     CDS             3791..4093
                     /locus_tag="VXD58_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV0675975.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4201..4839
                     /gene="udk"
                     /locus_tag="VXD58_001276"
     CDS             4201..4839
                     /gene="udk"
                     /locus_tag="VXD58_001276"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV0675976.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4967..6307
                     /locus_tag="VXD58_001277"
     CDS             4967..6307
                     /locus_tag="VXD58_001277"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV0675977.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6285..6497
                     /locus_tag="VXD58_001278"
     CDS             6285..6497
                     /locus_tag="VXD58_001278"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV0675978.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6494..7369
                     /locus_tag="VXD58_001279"
     CDS             6494..7369
                     /locus_tag="VXD58_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV0675979.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7362..8177
                     /locus_tag="VXD58_001280"
     CDS             7362..8177
                     /locus_tag="VXD58_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV0675980.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            8170..8601
                     /locus_tag="VXD58_001281"
     CDS             8170..8601
                     /locus_tag="VXD58_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0675981.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8608..10275
                     /gene="recN"
                     /locus_tag="VXD58_001282"
     CDS             8608..10275
                     /gene="recN"
                     /locus_tag="VXD58_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV0675982.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10277..11005
                     /locus_tag="VXD58_001283"
     CDS             10277..11005
                     /locus_tag="VXD58_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV0675983.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11084..12907
                     /gene="lepA"
                     /locus_tag="VXD58_001284"
     CDS             11084..12907
                     /gene="lepA"
                     /locus_tag="VXD58_001284"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV0675984.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(12988..>13228)
                     /locus_tag="VXD58_001285"
     CDS             complement(12988..>13228)
                     /locus_tag="VXD58_001285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0675985.1"
                     /translation="HSFEVVDETDEVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSS
                     KHGFEIVDETDEVSNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         3926 a         2433 c         3165 g         3704 t
ORIGIN      
        1 tatccttaat aaaatgatat ggaggtcacc ttatgacttg tgattttaaa tctgaaactc
       61 tacaactaca tgctggtcaa gttgtggctc cagctactaa gtctcgtgca gtgccgattt
      121 atcaaacaac attttttgtt tttgatgaca cgtaggaagg tgccgatctg tttgccttga
      181 ggaaaccagg gaacatttat actcgtatca ccaatcctac aacagctgcc cttgaaggtg
      241 gtgttgaagc gctagcaaca gcatcaggta tgactgcagt gacttatacg attttggcgc
      301 ttgcccatgc tggtgaccat gtagtggctg cttcgactat ttacggtgga accttcaatc
      361 ttttgaaaga accccttcct cgttatggta tcacaacaac ctttgtcgat gttgataatt
      421 tggaggaagt agaagcagct atcaaagaca ataccaagct tgtcttgatt gaaaccttgg
      481 gtaactcctt gattaatatt ccagacctgg aaaaactggc agagattgct cataaacatc
      541 aaatcccact tgtgtcagac aatacttttg caatacctta tttgattaac gtcttctctc
      601 atggcgttga cattgccatt cactctgcga ctaagtttat cggtgggcat ggtacaacta
      661 ttggaggaat aattgtcgat agtggtcgtt ttgactggat ggcttcaggg aaattccctc
      721 aatttgttga cgagggttca agctgccaca atttgagcta tactcgtgat gtgggtgcag
      781 tagcctttat tatagctgtt cgagttcaat tgcttcgtga tacaggtgca gccttgtcac
      841 cattcaatgc tttcctcttg ctacaaagac ttgaaacctc ttcacttcgt gtggaacgcc
      901 atgtacaaaa tgctgagaca attgttgatt ttcttgtcaa ccatcctaag gtagaaaagg
      961 taaattatcc aaaacttgca gatagtcctt atcatgcctt ggctgagaaa tacttgccaa
     1021 aagatgtcgg ttcaatcttt accttccatg tcaaaggtgg cgaggaagaa gcacgcaagg
     1081 tcattgatca tttagaaatc ttttctgacc ttgcaaacgc ggcagatgct aaatcgcttg
     1141 ttgtccatcc agcaacaacc actcacggtc aattgtcaga aaaagaccta gaagcagcag
     1201 gtgtcacacc aaactaaatt cgtttgtcaa tcggtcttga aaatgtagaa gatttgattg
     1261 aagacttgcg cttggccttg gaaaaaattt aaagtaaaag aagataaaca gtgggcttcg
     1321 actcactgtt tttgattttc cctcaggtat gatataatgg ttaaagaagt ctagaaagag
     1381 gaacgatatg aacgaaatca aatgtcccaa ctgtggggaa gtctttacag taaatgagag
     1441 tcagtatgcc gaactcttgt cccaagtgag aacggcagag tttgataagg aactacacga
     1501 taggatgaag caggaactgg ccttggctga gcaaaaggcc atgaatgagc aacagactaa
     1561 actggctcag aaggatcaag aaattgcgca attacagagt cagatccaaa actttgatac
     1621 agaaaaagaa ttggccaaga aagaggttga acagacaagc catgaggctc tcttggctaa
     1681 ggacaaggaa gtacagctct tagaaaatca gttggctacc ttgcgtttgg agcatgaaaa
     1741 tcaactgcaa aagacccttt ctgacctaga aaaagaacgg gatcaggtta aaaaccaact
     1801 acttttgcag gaaaaggaaa atgaattatg tttggcttct gttaagcaaa actacgaagc
     1861 ccagctcaag gcagctagtg aacaagtcga gttttataag gattttaagg ctcaacaatc
     1921 tacaaaagcg attggggaaa gcctagaaca gtatgcagag agtgagttta acaaggttcg
     1981 tagtttcgcc tttccaaatg cttactttga gaaggataac aaggtctctt cgcgtgggtc
     2041 taaaggggac tttatcttcc gtgagtgtga tgaaaatgga gttgaaatca tttctatcat
     2101 gtttgagatg aaaaacgaag cagacggaac agagaagaag cacaagaatg cagattttta
     2161 caaggaattg gacaaggacc gtcgggagaa gaactgtgag tatgccgttt tggtgaccat
     2221 gcttgaggcc gataatgact actttaacac agggattgtt gacgtcagcc acgagtatga
     2281 aaaaatgtat gttgttcgtc ctcaattctt tatccagttg attggtctct tacgtaatgc
     2341 ggcgctaaat tccctaaaat acaagcagga gttggccttg gttcgcgagc aaaatattga
     2401 cattacgcat tttgaggaag acttggatgc ctttaagcta gcttttgcta agaactataa
     2461 ttcagcttcg actaactttg gaaaagctat tgatgaaatc gacaaggcca tcaaacgcat
     2521 ggaagaggtt aagaaattcc tgactacatc tgaaaaccaa ctccgtctag ctaacaacaa
     2581 attggaagat gtctctgtta aaaaattgac ccggaaaaat ccaacaatga aagcgaagtt
     2641 cgaagcactg aagggggagt agaaagcaaa aatgaacggt attatcaact taaaaaaaga
     2701 agcaggaatg acctcgcatg atgcggtttt taaactgcgt aagattttgg gaaccaagaa
     2761 aattggtcat ggtggaacct tggatccgga tgtggtgggt gttttgccga ttgcggttgg
     2821 caaggcgaca cgcatggtcg agtttatgca ggacgagggt aagatctatg agggggaaat
     2881 tactctgggt tattccacga cgactgagga tgctagtggg gaagtgatcg cagaaacccc
     2941 tgttttgtct cccttggatg aaaagcttgt tgatgaagcg attgctagct tgactgggcc
     3001 tattacccag attcccccta tgtattcggc agttaaggtt aatggtcgca agctctatga
     3061 gtatgcgcgt gctggtcagg aagtggagcg tccagaacgt caggtgatca tttatcaatt
     3121 tgagcgaaca agtccgattt cttatgatgg ccaacttgcc cgattcactt ttcgtgtaaa
     3181 atgcagtaaa gggacttaca tccgtacttt gtcagttgat ttgggtgaaa agcttggtta
     3241 tgcggctcat atgtcccatt tgactcgtac tagtgctgct ggcttacaat tagaagacgc
     3301 tctttccttg gaggaaattg ctgaaaaagt agaggctggg caattagatt ttctccatcc
     3361 tttagagatt gggacaggtg accttgtcaa agttttccta actccagaag aggctacaga
     3421 agttcgcttt ggtcgtttta ttgagctaga ccaaacggac aaagaactgg ctgcctttga
     3481 agatgataaa ttgttagcca ttctagaaaa acggggcaat ctctataagc caaggaaggt
     3541 ttttagctag atcgtttagg aataaaaatc gggtgataga taacaattgc ttgataaaac
     3601 cccatactaa tagtagaatg gttttgggaa ttataatatt ccaattgttg cgagttgtag
     3661 gtactcaaat aatctatata gaaatttaga ggtgtgaaat gaagcaattt aaaattcttt
     3721 cagataaata tttagagtcc attacaggtt ctggtgggaa cttaggccca ggatttggtg
     3781 tgataattcc atgatgcgaa atgagtttcg agaaagggtg gagcaacttc ttcaacaaaa
     3841 agaaataaat gaaaatagtg agttgagtca cctgtttcgt cttgctatac aaaatttaga
     3901 cagaaatgaa aaataccaat cggtcatggc caatttgagt caagggttgt cactttacct
     3961 catgacgcat cattaccagg cacctaagtc tgtcattgat tttggtttat ggattgccaa
     4021 agctcctagc caggaaagag ggagactggc tttcttgcag atgcttgctc aaaccctaca
     4081 gggctttcgt taaaactgtc gaacatcaga gggaagactc tggtgttttt tgctgatgag
     4141 actataaatt ttgtaaaaaa tgtgatagaa tagacgacgg ataaaataac ggaggatagc
     4201 atgcaaaata gaccaatcat tattggagtg acaggtggtt ctggtggtgg taaaaccagt
     4261 gtttcaagag ccattttatc gcattttcct gatgaaaaga tttccatgat tgagcatgat
     4321 tcatactaca aggatcagtc tcatttgacc tttgaagagc gtgtcaaaac caactatgac
     4381 catccttttg cctttgatac agacttgatg atcgagcaga ttaaggaatt gttggcaggg
     4441 cgtccggtgg acatcccgac ctacgactat acagagcata cacggagtag caagacctat
     4501 cgtcaggagc ctcaagatgt ctttatcgtt gagggcattt tggtcttgga ggacaagcgt
     4561 ctgcgcgatt tgatggatat caagattttt gtggatacgg atgacgatgt gcgtattatt
     4621 cgtcgtatca agcgtgatat ggaggaacgt ggccgtagcc ttgatagcgt tattaaccag
     4681 tacttaggtg tggtcaaacc aatgtaccac cagtttatcg agtcaactaa gcgttatgct
     4741 gatatcgtca ttcctgaagg ggttagcaat accgtggcta tcgacctgtt gacgaccaag
     4801 attgcaaaga ttttggaaga agctcgaaac agcaaataat cacatgtgga ggccttgcct
     4861 cctttttcta tttttccctc ataatggtac ataaatggag atttttaggc atatttttgg
     4921 tataataata cccataaaag agcaagtaaa tgaatagtag gtggagatgg aaaagtattt
     4981 atcggtaaca actttgacca aatatctgaa aatgaaattc gataaagacc catacttgga
     5041 acgggtctat ttaactggtc aagtttccaa ctttcgtaaa cgacctactc accaatattt
     5101 ctccctaaag gatgaccatg cagttattca agcgaccatc tggtctggga tttatcagaa
     5161 attagggttt gacctggaag aaggaatgaa gatcaatgtg attgggcgtg tacaggtcta
     5221 tgaaccaagt ggtagctact ccatcatcat tgaaaaagct gagcctgatg gggttggggc
     5281 gcttgcgatt cagtttgaac aacttaagaa aaaattgaca gaagaaggcc tgtttcaaga
     5341 acgcttcaag caagctctac cccaattttc taagagaatt ggggtagtaa ccagccgtag
     5401 tggagccgtt attcgagata ttatcacgac cgtcagcagg cgattcccag gtgttgacat
     5461 ccttctttat cctaccaagg ttcaaggtga aggggctgcg gaggaaattg ctagaaatat
     5521 tgcgcgtgct aatcaacggg acgatttgga tttgctcatt attggtcgtg gaggtggttc
     5581 tattgaggat ctctgggcct ttaacgaaga aattgtggta cgagctattt ttgaatctcg
     5641 tttgccagtt atctctagtg tggggcatga gacggatgtg accttggcag attttgtggc
     5701 agatcgacgc gctgcaacgc caacagcggc ggctgaactg gcaacacctg tgaccaagtt
     5761 ggatgtatta actcatttgc aaaatcagga aaaacggatg gcaacggcag tccgaaatgt
     5821 tctatctaag aaacaagagg ctttgaaaaa atgcagtcag tctgttatct ttagacaacc
     5881 tgagcgcttg tatgacggtt atttgcaacg cttggaccaa ctgcaactgc gtttgaaaca
     5941 aagtttgcga actcggattt ctgataacaa acaattagtt caagcaagaa ctcatcaatt
     6001 agtacaatta tcacctgtta ccaaaatcca acgctatcaa gaccgtttag gacagttgga
     6061 caagctctta ggtagccaaa tggcgttagt ttatgacgcc aaggttgctg aggccaagcg
     6121 actttcggaa gctttgctca tgttggatac tagccgaatc gtggcgcgtg gttatgctat
     6181 tgtcaaaaaa gaagagtcca ttgtagattc ggttgagagt ttgaagaaaa aagaccaagt
     6241 aacgcttttg atgcgagatg gtcaagtaga attagaggtt aaagatgtca aaacaaaaga
     6301 aatttgagga aaatctagca gaactggaaa ccattgtcca aagtttggaa aatggtgaaa
     6361 ttgctctgga agatgcgatt actgcctttc aaaagggcat ggtcttgtca aaagagctcc
     6421 aagctacgct ggacaaggct gaaaagacct tggtcaaggt catgcaagaa gacggaacag
     6481 aaagtgattt tgaatgaaaa agcaagaaaa attagctctt gtcgagtcgg ctttggaaga
     6541 tttttatgga gaccagcagt ttgcctctag tttacgggag tctgttctct attctattca
     6601 tgctggtggc aagcgtattc ggccttttct cttgttagaa gttctggaag ccttgcaggt
     6661 taccatcaaa cctgctcacg cgcaggtagc tactgccttg gagatgattc atacagggag
     6721 cttgattcac gatgaccttc ctgctatgga tgatgacgat tatcgaagag ggcggttaac
     6781 caatcacaag aaattcggtg aagctatggc cattttggct ggagatgcct tattcttaga
     6841 cccatatgcc ttgattgcgc aggcagattt gccaagtcag attaaggtgg acttgattgc
     6901 caacttatcc cttgcttcag gtagtctggg tatggtggca gggcaagttt tggatatgga
     6961 gggcgaacac cagcacttgt ctttggaaga acttcagatt attcatgcca ataagactgg
     7021 gaagttacta gcctatccct tccaagcggc agctattata gctgaattgt cacctgaaat
     7081 gcaggtgaag ctgaaaactg tgggtgaatt gattggactt gcttttcaag tcagagatga
     7141 tgtactggat gtgacagcta gttttgagga aatcggcaag acacctcaaa aggatctgca
     7201 ggcagaaaaa tcaacctatc ctgccctgtt gggcttggaa gagtccattg ccttttgtaa
     7261 ccaaaccctg gatcaagcta atgaaaaatt agaagaaatt gcccagcagc ttccctttga
     7321 aacagaatcg attgtaagtg tagtagaaag tttgagaatc aatggctaag gaaagagtgg
     7381 atgtactagc ttataaacag gggttgtttg aaacgagaga gcaggccaag cgaggtgtaa
     7441 tggctggcct agtcgtagca gtccttaatg gagaacggtt tgacaagcca ggagagaaaa
     7501 ttccagatga caccgaatta aaactcaagg gagagaaact caagtatgtc agccgtggtg
     7561 gtttgaaact ggaaaaggcc ttgcaggtct ttgatttgtc ggtggatggc gcgactacga
     7621 ttgatatcgg ggcctctact ggaggtttta ccgatgtcat gctacagaat agtgccaagt
     7681 tggtctttgc agtcgatgtt ggtaccaatc agttggcttg gaaattacgc caagacccac
     7741 gagttgttag catggagcag ttcaatttcc gctatgctga aaagactgat ttcgagcagg
     7801 agccgagctt tgccagtatt gatgtgagtt tcatttccct tagtctgatt ttgccagcct
     7861 tgcatcgtgt cttggctgat caaggtcagg tggtagcact tgtcaaacct cagtttgagg
     7921 caggacgtga gcagattggg aaaaatggaa ttattcgaga tgctaagatt catcagaatg
     7981 tccttgaatc tgtaacagct atggcagtag aggcaggttt ttcagtcctt ggcttggact
     8041 tttctcccat ccaaggtgga catggaaata ttgaattttt agcgtatttg aaaaaagaaa
     8101 agtcagcaag caatcagatt tttgctgaga ttaaagaagc agtagagagg gcgcatagtc
     8161 aatttaaaaa tgaataaaaa agagagactt gaaaaaatta gaagattggt gacagattat
     8221 caaatcggca cgcaagaaga aattgtagaa catttgaaag aagcaggtat cactgccact
     8281 caggcgacgg tatcccgaga tatcaaagag ttaggtattg tcaaaattcc tttgagagac
     8341 aacacctatg tctacgaatt accaaaatca atcgtaaaaa gtctgcaact ggctgaagac
     8401 aatatcgaat cggctgaatt gatggataag atgatcaatc tccaagttat tccaggaaat
     8461 acggcttttg taaaagctca gttaatcgat acttttgcag acaagatttt tagttgtttg
     8521 actgatgata gctcgatttt agtcattgcc agaagtggaa gtctagcaga ggaaatcttt
     8581 gaacaagtaa aaaattggta ggtctatatg ttacttgaaa tttcgataaa aaactttgcc
     8641 attattgagg ctatttccct caattttgaa aaggggatga ctgtcctgac tggtgaaacg
     8701 ggtgcaggga agtcgattat cattgatgcc atgaatatga tgttgggagc tcgtgcgacg
     8761 acagatgtta ttcgtcatgg tgcaccaaag gcagagattg aggggctttt ctcagttgag
     8821 aatagtcgtc ttttacagga aatttttgat gagcaaggtt tagaattagg tgatgaaatt
     8881 atcatccgtc gggaaatctt gcaaaatggt cgtagtatca gccgtgtaaa tggtcagatg
     8941 gttaatctgt ctgttttgcg agctattggg caacatcttg tagatattca tggtcagcat
     9001 gaccatgagg agttaatgcg tccccaactg catatccaga tgttggatga atttggtgat
     9061 gccgcttttt gggacttgaa agaaacctat caaacgagtt ttgatgccta tcggaaaatg
     9121 cgcaagcagg ttctggaagt caagaaaaac caacaggaac acaaggaacg tatcgaaatg
     9181 ttggaatttc aaatggcaga gattgaggca gcaaacttgc aggctggaga agacttggct
     9241 ctcaatcaag agcgagataa actcctcaac cacaaaaata ttgcggatac actgaccaat
     9301 gcctacagta tgttggacaa tgaagatttt tcaagtctgg ccaatgttcg ttcagctatg
     9361 aatgacatgg aaagtgtcga agagtatgat cctgaatacc gtgaaatttc aagctctctg
     9421 tctgagacct actatgtttt agaagacatt agcaaacgtt tggaagctat cattgaggac
     9481 cttgattttg atggcaatcg cctgatgcag gttgagaatc gtttggacct ccttcatact
     9541 attactcgta agtatggtgg gactgttgat gatgttttgc tttattttgc caagattacg
     9601 gaagaataca atctcttgac aggcaataat ctttcgtctg aggacatgga agcagagctt
     9661 aagaagttgg aagtcaatct tgtcaatttg gcaggtcaac ttgcttctgc tcgtcataat
     9721 ttggctcagc aactcgaagc tgagattaaa caagaactgc aagatcttta tatggaaaaa
     9781 gcccagtttc aggttcattt tagtaaggga aaattcagtc gtgggggaaa tgaaatggtt
     9841 gagttttata tttcaaccaa ccctggagaa gactttaaac ccttggttaa ggttgcttct
     9901 ggaggggaat tatctcgtct catgttagcc attaagtctg ccttttcacg taaagaaggc
     9961 aagactagca ttgtctttga tgaggtggat acgggagttt caggtcgtgt tgctcaagct
    10021 attgctcaga aaattcataa aattggtcag catggtcagg ttttggctat ctcccatttg
    10081 ccacaagtaa ttgcgattgc agattatcaa ttctttattg agaagattag taatgaccat
    10141 tcaacggttt cgactgttcg tctcttgaca gtcgaagagc gagtggagga agttgccaag
    10201 atgttggcag gtgatgatgt gacagaagca gccctgacgc aagccagaga attgttgaga
    10261 aacagggaga aataagatga cagattatta tgtaattgga gatgttcacg gaaaagctgg
    10321 gatgctggaa gaccttctta aaacctggga tggtcagacc cagttgctct ttctaggtga
    10381 tttgattgac agaggtgagg atagtcaccg tgttcttgaa atggtcaagg acttggtgga
    10441 caatcaaggg gctatctgtt tgtctggaaa ccacgagtat atgtttttga cttggctaga
    10501 tgacccagaa gaaagttatg accattatcg tcgcaatggt ggagatacaa ccattaactc
    10561 tatcctaggt cgtcccttgg atgcaccagt tgatggagta gaagatgcca agcgggttgc
    10621 cgctgaagcg tcagacttgg tcgaattcat tcgtcaaatg ccatttgtag tagagacaga
    10681 caagtatata tttgttcacg caggtattga tttgactttg gacgactggc atgaaacaac
    10741 agattataaa aaagtatggc ttagaaaacc attccacgga gctgaaaatc atactggaaa
    10801 aaccattgtc ttcggtcata caccggtcta tggtttgtta cagcaagaac gcggtacatc
    10861 tgagctttgg actacagaag atggcaagat tggaatggat ggaggagctg tttatggtgg
    10921 tgtccttcac gggattgtct ttacagacca aggaatgaca gaacaccact ttatcgaaaa
    10981 tgacggcttt gtcgctgaag attagtactc ctagcagggt atggtcttgt caaaatatca
    11041 aaaacaattt ataataaata gataccctga aaggaagaga atcatgaact tagaagaatt
    11101 gaaaaaacga caggggaaga tccgtaactt ctctattatc gcccatattg accacgggaa
    11161 atcaactcta gcagaccgca ttttggaaaa aacagagacg gtttcaagtc gtgaaatgca
    11221 ggcccagctt ttggatagca tggatctaga gcgggaacgt ggaattacta ttaagttgaa
    11281 tgccatcgag ttgaattaca ctgcaaaaga tggggaaact tatattttcc acttgattga
    11341 cacaccaggg cacgttgact ttacctatga agtttcacgt tcgctagctg cctgtgaggg
    11401 tgctattttg gtggtcgatg cggctcaagg aattgaggct caaactcttg ccaacgttta
    11461 tctggctttg gataatgatt tggaaatcat gcctatcatt aataaaattg acctgccggc
    11521 tgcagatccg gagagcgtgc gtacagagat tgaagatgtg attggtttgg atgccagtga
    11581 agcagttttg gcttctgcca aggctggtat tgggattgaa gaaatcctcg agcaaattgt
    11641 agaaaaagta ccagcaccaa cgggtgatgt gacggcgcca cttaaggcct tgattttcga
    11701 ctctgtttac gatgcttacc gtggggttat cctccaagtg cgtgtcatgg acggagtggt
    11761 caaacctggt gataagattc agctcatgag caatagtaag acctttgatg tggccgaagt
    11821 cggtattttt acaccaaaag cggttggtcg tgatttcctt gcgactggtg atgttggtta
    11881 cattgcggcg tctatcaaga cggttcagga tactcgtgtg ggtgataccg ttaccttggc
    11941 aaccaatcct gcggcagaac cattacatgg ttataagcag atgaatccta tggtctttgc
    12001 gggtctctac ccaatcgaat caaacaagta caatgacctg cgtgaagcgc ttgaaaaatt
    12061 gcaactgaat gatgctagtc ttcagtttga accagaaaca tctcaggcac ttggatttgg
    12121 tttccgttgt ggatttcttg gacttctcca tatggatgtt atccaggaac gtttagagcg
    12181 tgagttcaat attgatctca tcatgacagc tccgtctgtt atttacaaag ttaatttgac
    12241 cgacggtgag tctatggatg tgtctaaccc atctgagttt ccagacccaa ctaagattgc
    12301 gaccattgaa gagccttatg tcaaggcgca aatcatggta ccacaggagt tcgtcggagc
    12361 agtaatggag ctagctcagc gtaagcgtgg ggactttgtg actatggact atattgatga
    12421 taaccgtgtc aatgttatct atcaaattcc tcttgctgaa atcgtctttg acttctttga
    12481 taaacttaag tcttcgacac gtggttatgc aagttttgac tacgaattgt cagaatatcg
    12541 cccatctaag ctagtgaaaa tggatatcct tctcaacgga gacaaggtgg atgccctcag
    12601 ctttatcgtt cataaggact ttgcttatga acgtgggaaa ctcatcgttg ataagctcaa
    12661 gaaaatcatc cctcgccaac aatttgaggt tccaatccaa gcggctattg gacacaagat
    12721 tgtcgctcgt actgatatca aggcccttcg taagaacgta cttgctaaat gttatggtgg
    12781 tgacgtttct cgtaagcgta aactccttga aaaacaaaaa gctggtaaga aacgcatgaa
    12841 atccatcgga tcagttgaag ttccgcaaga agccttcctc agcgtcttga gtatggatga
    12901 agaataagtt atcagaaatg cctttccttg ttcacaggaa atttatataa gaatcaatag
    12961 taaagcaggc ttagatggtc tgctttttta gtttttatac tcttcgaaaa tctcttcaaa
    13021 ccacgtcagc gtcgccttac cgtatgtatg gttactgact tcgtcagttt catctacaat
    13081 ctcaaaacca tgttttgagc tgacttcgtc agtttcatct acaacctcaa aaccatgttt
    13141 tgagctgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc tgacttcgtc
    13201 agtttcatct acaacctcaa aactgtgt
//
LOCUS       DAVLFB010000050        13085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLFB010000050 DAVLFB010000000
VERSION     DAVLFB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.050"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(10..1827)
                     /locus_tag="VXD58_001286"
     CDS             complement(10..1827)
                     /locus_tag="VXD58_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV0675986.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(1925..3067)
                     /gene="tgt"
                     /locus_tag="VXD58_001287"
     CDS             complement(1925..3067)
                     /gene="tgt"
                     /locus_tag="VXD58_001287"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV0675987.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            3197..4054
                     /locus_tag="VXD58_001288"
     CDS             3197..4054
                     /locus_tag="VXD58_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV0675988.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(4082..4726)
                     /gene="pcp"
                     /locus_tag="VXD58_001289"
     CDS             complement(4082..4726)
                     /gene="pcp"
                     /locus_tag="VXD58_001289"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV0675989.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(4833..5192)
                     /locus_tag="VXD58_001290"
     CDS             complement(4833..5192)
                     /locus_tag="VXD58_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV0675990.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(5203..5628)
                     /locus_tag="VXD58_001291"
     CDS             complement(5203..5628)
                     /locus_tag="VXD58_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV0675991.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(5951..7063)
                     /locus_tag="VXD58_001292"
     CDS             complement(5951..7063)
                     /locus_tag="VXD58_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV0675992.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(7229..7849)
                     /locus_tag="VXD58_001293"
     CDS             complement(7229..7849)
                     /locus_tag="VXD58_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0675993.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(7853..9133)
                     /locus_tag="VXD58_001294"
     CDS             complement(7853..9133)
                     /locus_tag="VXD58_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV0675994.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(9194..10678)
                     /locus_tag="VXD58_001295"
     CDS             complement(9194..10678)
                     /locus_tag="VXD58_001295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV0675995.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(10754..12031)
                     /locus_tag="VXD58_001296"
     CDS             complement(10754..12031)
                     /locus_tag="VXD58_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV0675996.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(12138..12221)
                     /locus_tag="VXD58_001297"
     tRNA            complement(12138..12221)
                     /locus_tag="VXD58_001297"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12185..12187),aa:Leu,seq:caa)
     gene            complement(12231..12302)
                     /locus_tag="VXD58_001298"
     tRNA            complement(12231..12302)
                     /locus_tag="VXD58_001298"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12268..12270),aa:Gln,seq:ttg)
     gene            complement(12316..12388)
                     /locus_tag="VXD58_001299"
     tRNA            complement(12316..12388)
                     /locus_tag="VXD58_001299"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12353..12355),aa:His,seq:gtg)
     gene            complement(12403..12473)
                     /locus_tag="VXD58_001300"
     tRNA            complement(12403..12473)
                     /locus_tag="VXD58_001300"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12439..12441),aa:Trp,seq:cca)
     gene            complement(12478..12558)
                     /locus_tag="VXD58_001301"
     tRNA            complement(12478..12558)
                     /locus_tag="VXD58_001301"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12522..12524),aa:Tyr,seq:gta)
     gene            complement(12571..12643)
                     /locus_tag="VXD58_001302"
     tRNA            complement(12571..12643)
                     /locus_tag="VXD58_001302"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12608..12610),aa:Phe,seq:gaa)
     gene            complement(12647..12720)
                     /locus_tag="VXD58_001303"
     tRNA            complement(12647..12720)
                     /locus_tag="VXD58_001303"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12684..12686),aa:Met,seq:cat)
     gene            complement(12731..12820)
                     /locus_tag="VXD58_001304"
     tRNA            complement(12731..12820)
                     /locus_tag="VXD58_001304"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12782..12784),aa:Ser,seq:tga)
     gene            complement(12903..12976)
                     /locus_tag="VXD58_001305"
     tRNA            complement(12903..12976)
                     /locus_tag="VXD58_001305"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12940..12942),aa:Ile,seq:gat)
     gene            complement(13011..13081)
                     /locus_tag="VXD58_001306"
     tRNA            complement(13011..13081)
                     /locus_tag="VXD58_001306"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13047..13049),aa:Gly,seq:tcc)
BASE COUNT         3963 a         3052 c         2354 g         3716 t
ORIGIN      
        1 taatacaagt tattttgatt taaccggctt gtcttgagct gtctgcaaag ctgtggcaat
       61 cgtatctgca tacaattttg ctcctgcttc gatagtgcta ctctcactcc cgaaatgaac
      121 ctggtctgtt ccagcccaaa tttctggatg ctctttcgca acttgattcc aatctgctat
      181 cgtaatgtaa ggtgtcttct ctgccaattc tctcatatag gcagcagcct tctcaacgat
      241 ggcataggtc tcttttgtct tatctccctc ataaggagtc accaaaatca tatggtgtcc
      301 cttaggaaga tttttcacga tactgtccca gtcatccttg taattctcag gattatttac
      361 cccagtcgca atgaccaccg tcttaggtaa aaatttattc tggctattat ttagcatgat
      421 ttcatttgcg gtcttggttg ttacgctgac ctgcgcgtta atctgtgctc caggaagagc
      481 tgtctgtagt gctgtatttg cccttaaagc cactgagtca ccaattaaca tagtgccatc
      541 agcaattccc aaactgtttg catctgcccg ttctgccatc accttggtct ggccaatatt
      601 tgttgcagct tgcttcaagc cattgacagt caagtctgtc tcaaacgctc ccacttgtgg
      661 tgccaacaag gtcaccgtgc agacaatgat ggtcaagatt cctgtacctg ctacaagaat
      721 tgcgtgaata taaggcaggg gacgaagagt ttgtaaaata ggtgtgttct tgcctgcaat
      781 ccaaggttcc aatacataga atgacagact ggcaaagcca taagaacaaa tcagagtcag
      841 taatacagca agaagatttg atgtcaactg tgagaaaatg atatagaaag gccaatggaa
      901 aagataaacc gcatagctag tatccgctaa aaagctgata atcttcggct cctgtatatg
      961 atgtgtcttt tcatgtaaga cacgcgccgc cagaatcatg gcaagagctg caagactggc
     1021 aagcaagaag ccgataagat aggcaaaaag ataagtgaat ttgacaaaga aagtcaaaag
     1081 aactaggaaa ccaaagcctc ctccaaaaac taccaaagtc tttcgtaaat cccagatttt
     1141 atccaactgc ttgacgaggg aagtcgtctg acgaacacct acaatagttg ctaacatact
     1201 tcccaaaaag aatggataga catgagttaa actggagaaa taaacagagg aataagaggt
     1261 cactagaaaa ctaccaataa acatggagaa gaaactgatc aagaaggcag cagcagataa
     1321 gagaaagacc atccccttca actgaccatt tgatttagct tgtttggata agaaccaaac
     1381 tgccaatccc caaagaatat agtagtgaac ctcaactgcc aaactccaat tatgaacaaa
     1441 caaatgagga atgaactgag attcataact cccacctgtt aggagttcat agaagttggt
     1501 cataaagcct aagacgcccg caatctggcc accaattcca gcaacatagt cttggcgaac
     1561 caagaaagta aaaggcatgg tcaccaagac catcaaaacc acaggtggca caatccgata
     1621 aaagcgtctt ctaaaaaatc ctatcaaatc aatctcattg ttcttagaaa attcttcaat
     1681 gagtagagct gtaatcagga aacctgaaaa tgtgaaaaag acatctaccc cgaaaaatcc
     1741 tccaggaaag atcgtctgaa agaagtgata caagagtacc aaaagtaaac ctataatcct
     1801 aatcaaggaa aaccatttaa tgcgcatacg agtttattct tcctttcatt gtacacttta
     1861 ttctatcaaa aaaagaagaa aaatcctaag agaaaactta ggattttttt atatcaattc
     1921 cattttagaa attacgtcct gacttattat agccatattt ttccacaaaa tactcacgga
     1981 attccaagag attgtcatcc atgatggctt gtcgcacttg cttcatcagg ttaagcaaga
     2041 agtaaagatt gtggtagcta gtcaagcgga taccaaaggt ttcatcagcc ttgagcaggt
     2101 gacgaaggta agcgcgtgta tagttattac atgtgtagca gtcacactca ggatccagtg
     2161 gcgtaaagtc ctcagcaaac tgggcatttt tcacaaccaa acgtccttga ctggtcatac
     2221 aagtcccgtt acgagcaatt cgagtcggta agacacagtc aaacatatcc accccacgaa
     2281 tgaccccatc gatcaagcta tctggcgctc ccacacccat cagataacga ggtttatttt
     2341 caggtagcag ttgagttgta aagtccaaga ccgcattcat ctcttcatgg gtttctccca
     2401 ctgccaaacc accgatagag tagcctgaga aatccatgct gacaagatca tgagctgatt
     2461 ggcggcgaag gtcttcaaat cctgcacctt gcacaattcc aaacaaacct tggtcatgtg
     2521 gacgacggtg agccttcaaa ccacgctcag cccaacggct ggtacgctcg atcgatttct
     2581 taacgtagtc ataaggttga taaaactgag gacattcatc aaaggacatc atgatgtctg
     2641 aacccagatt attctgaata gagatggctt tttctgggga taggaacatc ttagaaccat
     2701 ttagatgatt tttaaaggtt actccttctt ctgtgatatt acggctatct gctaaagaat
     2761 aaacctgaaa accaccacta tctgtcaaga taggctggtc ccaattcatg aacttgtgga
     2821 gaccaccagc gcgtgcaatg agttcatctc cagggcgaag ccagagatga taggtgtttg
     2881 ataggataat tcccgaaccc atctccttca attcttcagg tgactgagtt ttgacagtgg
     2941 cttgtgtccc aactggcata aacataggtg tcggaaaggt accgtgggga gtgatgattt
     3001 ctcccagacg agctcctgtg tgtttttctt tcttaatcaa acgatatttg attggtgaat
     3061 ctgacatttt ttacctccga agctgggaaa gacagtccca gttcatacag tgtgcccaag
     3121 gacacactgt atgattttat caaaaaaagt aggaactgtc acgaactatg tataagaact
     3181 atggtataat aagagaatga actatccaaa aattgattta aaaacaattc gtcaggagtc
     3241 aaaacatttt caggctgaca ccccccgtct ctttctcctc tatatccttc ccagtatgct
     3301 ggtcatctta tctggttttc ttaatccctt atctcggatc cacgggactg ttttagaaca
     3361 gccatttttc agcatacttg gccaaatact tcaaacctac ctctttcctc tactagtctc
     3421 ctttatcggg actattcttt tgaccagttc cgtctatgca acactgacac tcatgaaaga
     3481 ttctaaaacg gagccatctg taaaaaacag cctagctctc tttgatgagg agcgcttttc
     3541 tcaaaccttt ttgaccctac ttctcaaacg tttctatctc tttttatgga gtatccctaa
     3601 tttacttggg atttacctcc tcttttatag tagcttttta gccaaaaaat tcgtcaccct
     3661 acatcctgag tttccaaatc tagatctctc atcagttgaa accgagcgtt tcctcatggt
     3721 ctttggactc tactttcttg caagtctcat cttgatcatt gtaggaaata tcctctatat
     3781 tccacagtat tatgcctatt cacaggtgga atttcttctc tgttatagtc tagacctagg
     3841 acaggtccca ccaagacgaa tcctaaaaac tagccgttcc tttatgaagg gctacaagtt
     3901 ccagcacttt gtcctagatt tacagcttct tccttggtac ttcctcaatt ggattacctt
     3961 tggcattgct agtttttcac tcctacctta tatccaatgc actaaaatta tgttttaccg
     4021 agcagtattg gcccgaaaac gtccaaaagc ttgaaggttt ttctcctcaa gcttttttct
     4081 atcaatgagt ttctccgcct accaacttga gttcctgatc tccatgttct attatagcgc
     4141 cgattgctgc ttctatccct cgccgaatat ccactaaact catagctgga gtagtcggtc
     4201 tgttcaccac ctgttccatc atataaggaa tatgcataaa acctgcctta acatatggag
     4261 atttcttttc taccaaatag agagcctgat acatcaaatg gctgcagaca aaagtccctg
     4321 ccgtattgga aacagaggcc ggtaagccct ctttttttat agcttgaacc atcgctttaa
     4381 tcggcaaact actaaagtag gccgaagcac catctgggcg aatgggacgg tcaatcggtt
     4441 gattatcttc gttatcagaa atgcatgcat cgtcttgatt aatggtcact cgttcaggtg
     4501 tcaaactagt tcttccacca gcttgcccaa tacaaaggac aaagtcaggt tgataacgat
     4561 tcatctcttc ttccaatact tgagcagatt tgtgaaaaac tgtcggcacc tctagccaac
     4621 ggacctcagc accatggatt tcagctggta aacctttaat ggcctccaaa gctggattgc
     4681 ccttttcccc tccaaagggc tcaaaacctg tcactaatac tttcatttct aactcctcaa
     4741 aaccaattca atgagacttt tttcttaaaa acaagtataa catatttccc ttattctgga
     4801 ttaaaaagga ttagcgagat tagaatcgac cttcaagtgt atttaaagag taaaatacta
     4861 atcaaggcca aaatagctga tccaccttgt ttcaaaataa tttttttatc cgctgttaaa
     4921 gagccgtaag tcgcagcacc aatcacaaat aagacaaaaa tagtcacaat ttctaaattc
     4981 tgtgagaaat aaatgacata caagagaaag actcctagca gagccttata aattccttga
     5041 tttttgaaca atgaacttac tgacggatga gccaattctt ccttttccat attaaataca
     5101 cgactagtcg catctgattg cgtggcaata ctttccaaat aaaaaatgta aaaatgctcc
     5161 aaagcaacga tcgttgttaa aataattgac atctatttct ccttaaattt ctatattttc
     5221 aatacccaaa accaatttat ttaataaacg gctgagttct gttctctcaa actctgttaa
     5281 gatactttct atctcttcct taaccctgat atgttgcaga gggggattca ccactaactg
     5341 ctccttggca tacttcgcag cctctaccaa cacttcccgc tgattttcag gattacgatg
     5401 acgctccacc aaaccttccg tttccaaaat tttgaaatgc cgtgtcaaag cagcctgatc
     5461 aattttcaaa cgctcctgaa ccgccatttg gttacaagga gaatgctcca gcaaaaacag
     5521 taaaatctga taccgtgtca aactaatccc aagccttttt tcaaacaatt gcgtactcgc
     5581 ttgctcagac aagcggagtt gatacagtaa atcttgaatc tctagcattt cttcccctct
     5641 ctttttattg atttatcaat atttgactaa ttaattgtga tataaagtaa aatagattgc
     5701 aagaatgttg cttaactcat tacaagatga taactttcca ttttccattc aaattgatac
     5761 tcaatgaaaa tcaaaaagca aactaggaag ctagtcgcag gttgctcaaa gcacagcttt
     5821 gaagtttcag ataaaactga cgtggtttga agagattttc gaagagtatg acaaagaaaa
     5881 aacgaacgag aatcgctttc catcacgaaa gctagatctc attcgtcaaa atgatgttat
     5941 caagtcgggt ttatccattc attgaaacgt gaacgtgatc atagtgattt tctgtcacac
     6001 taccacggtc tggcattggg ttccaagtgt tagctggccc atatttgcta tcgaatggag
     6061 catagaaacg ttgtttccag atgatgtaac taatgccacg gctggccata ttttgaatag
     6121 catattccgc aatcttatcc cctaattctg aacgttctgg taccataaag tcgatagcca
     6181 aaccttttcc gtgatctcca ctgtctcctg gacgataacc actaaaggat gtaatgccaa
     6241 acaagttagc aatttcttct ttaaaggcag ctgtttgtgg ttgaagacct gcattttcag
     6301 attttgctac tgcaagtcca gcataatcgg gcgcagctgg agcctcgtaa gttgttgaaa
     6361 ttattttcgt ctcttctggt tgataagtag aaactgctgc tgtagctcca tttgatgatg
     6421 ctacttcttt tgcttctgaa cttgttgtag ttacttccac tgctgatgca gcttgagggc
     6481 ttgcttgtgt ttcctgcttc tcagctggag tcgtttcctg aggagttgct tcttcaactg
     6541 ggcgagttgt ttcggtcgtt tgctcctctg ggacagaagt gcccgtagat ggtgccactt
     6601 cttcagaagc aatcactgtc tttgtaactt ctgcaattgg ttgagaaagg tctgcaacct
     6661 gaacagtttg atcatcaacg gtcacttgat tagtggtcaa atctgctgtc gcagttgtca
     6721 cttcttcact agagtctgct tgaggtgttt ggatttcaac ttctgttact tcttctgctt
     6781 cattgacagt cgttgtcaaa acagtttctg ggaaaatcaa gtccatatta gtgattttgt
     6841 tcagattcgc aagcactgtt acatctacac ccaaggcttc tgcaatggtg ctcaaagtat
     6901 caccatactg tacggtataa cttgttttgt tgtccgtttt agtcaaatcg ttttggattt
     6961 gctcaacact acgtgcagtc caaagaactt cttctgcttg agttgccaat actggggcaa
     7021 atgacaaggc tactgttgac gctaataaca ttcttttctt cattctttca aattcctttc
     7081 aaatgagtac ctgtctatca taacacaaaa aatgtagaaa aaaagccctc tcgggcctat
     7141 ttaacagaac tgtctattcc ttgtaacaaa ctaggttact tgaaatagtt tgttaggtct
     7201 ctgtcacaaa actgacacaa tctttttatc acttagtctc aaaaatacgg gaaatatctg
     7261 ctaacgcttg aatcctgtga ttcccttcat aagtagactc taaaaagttg atgctttgaa
     7321 tcccactatt ctgggcaaat tccacatcca gagtccgatc ccctatataa taagtattat
     7381 cagaattcaa ctgatactta tctagcagat aggtagccgc ttctggactt ggcttccgca
     7441 caaagccact ctgactggtt aaaatctctg taaaatagga ttccaccccc aagtccttga
     7501 gaatggtaaa agcgttgttc cccttatgag tatatataaa ctgctgaatt cctgattcgt
     7561 ctgcccaagc tagcacctca cgcgcacctg gcatcaaaac tacctgagca ttcttctcag
     7621 ccagactctg ggcacgcacc tgatttagca cctcaacatc cagatttcta tcttctgcca
     7681 cccgcacaag caaatcttgc accgaatact tgaagataaa ctctctcacc ttctccttat
     7741 cataaggaat agaaaactga gcaaaagtct cctcaatccc tgataaaatc gcttcgtaag
     7801 agtccaataa agtcccgtct aaatcccaaa taaaagctgt tttttgcatt tcctatcctt
     7861 tcaagctcat atagcgctgg taacgatagc gtagaaataa ccagcgaaaa ccattatcca
     7921 agagagaccc tgcccaaata ccaggcaagc cccaaccaag cacaatcccc atcagatatc
     7981 ctgtcccaat gcggatacac cacattccta tacttgtcgc ataaaaaggg aggcgtgcat
     8041 ttcctaatcc ctgccagact gccgtataga tgactgttcc tgtcgtcata ggggtcccaa
     8101 gtagtgaaaa cagtgtcact agaacactag cctccaccgc tagagaatca gtcgtataga
     8161 gatgagttaa tggtacaccc aagacatata tactaaagga caggggcaac atgaggaaca
     8221 gagaaagcca aaaggtttgt ttactcaaac tagcaactct tttccaatca tcctctccaa
     8281 ctgctcgggc caacagcatg accgttgccg tagcgacgcc aaaggcaggc atatagttaa
     8341 actgggtcaa gacttctccg attgcattcc cagcaactgc ctccgtccca aaagaaacga
     8401 ccaaggcaat gatcactaca tctccagccc tcatcataag tcgctctcca gctgctggta
     8461 aagccaaggt caacagttcc ttatctaaac caaaagttgg cttcccataa ggcagtttta
     8521 attgtgacca caaaatcaca agaccaacca aacgagacac aattgtcccc caagcaacac
     8581 cagctatccc catatccaga acaaaaatag ctagacttga aaaaagaata ttcaaggcat
     8641 tggataaaaa actaacatag agaggcagac gtggattatg cgttgcacga atcaaggctc
     8701 ctagactagt cattaaacct aagagaacaa tcgatccgcc taccaaagat agatacagtc
     8761 caccactctc agctacatcc ctctccgtcc ccaaaagtcc tatcatctct ttcccagcga
     8821 agatggacaa aaatcctaaa aggaaactta atagtaaggt aatcttcaac gcctcagtca
     8881 catgataggc caacttcgac tggtctttct gccctatgct ttttgaaata acactggaaa
     8941 tagcagctcc cagagcgatg aaaatcgcct gataaatggt gataatatta ccagctactg
     9001 aaacccctga aatagctatc aatcctaaat gagcaaccaa ataactgtcc accattccca
     9061 ttagcatctg caaaaagttt tcacccatag ctggcaatgc aatattaaga atgtctttat
     9121 ttttcttaaa caatctctcc tcctgatgaa aagaaactca gttggtttcc caaccgagtt
     9181 tgctccctct gtcttaaagt cctaaataag cctcaaccgc tgcttgcatg tcagcagctg
     9241 ccactgttgt cttgtgacga attggagcta tttcaagccc atcaactgct ggtggcactg
     9301 caacgcctga gatttcatgt aattgagcca aggcttcaaa gtctgttaaa cctgcttttc
     9361 cagttacagc ttctactgca actactggga acttgtatgg actagctgtt gaagcaatca
     9421 ctgtcttagt tacatctcca gtggccgatt ggtatttttt ataaactgct gaagcaacag
     9481 ctgtatgagg gtcctcgata taagaatcta actcacaaac acgcttgatc tctgctgccg
     9541 tttcttcctc agtcgcatat tcagctgcaa agaggtccaa aatctctgca tcaaagtctg
     9601 tcaacttata ttgtccttgc gtgttcaagg cattcataag ttcagttgtc ttttcagcat
     9661 tatttcccaa aagatggaaa atcaagcgct ccaagtttga agataccaag atatccatag
     9721 atgggctggt tgttacctta aactcacgtt ttttgtcata gacacgtgtt ttaaagaagt
     9781 ctgtcaaaac attgttgtca tttgaagcac agattaattt accaactggc aaaccaattt
     9841 gtttggcata aaaggcagcc aagatatttc caaagtttcc tgttggtact gtgaagttaa
     9901 ccttttcacc agctacaatt tcaccagtct taaccaattg agcgtaagca taaacataat
     9961 aaacaatttg tggcaccaga cgaccaatgt tcatagagtt agctgatgaa aattgcaact
    10021 tgttggtagt caatttttca cgaagagcca cgtcgttaaa catgtgcttc acatttgttt
    10081 gcgcatcgtc aaagttacca tcaatagcaa taacatgagt attgtcgcca gtctgagtgg
    10141 tcatttgtaa ctcttgaatc ttgctgacac catcctttgg ataaaagacg ataatctcag
    10201 taccaggcac attcgcaaac cccgccatag cagctttccc cgtgtcacca gatgtcgctg
    10261 tcaagataac aatcttgttc tccaaaccat gtttcttagc agcagtcgtc ataaagtatg
    10321 gcaaaataga caaggccata tccttaaagg caatcgttga accatggaaa agttccaaat
    10381 tgtattgccc atctaatttc actaatggtg caatagctgg agtatcaaat ttgctatcgt
    10441 aggcattgtt gatacagtag tccaactcct caactgtaaa gtcatctaaa aatgctgaca
    10501 aaactagctt agcaacttcc tggtaagaag catctttcaa tttgtcaaag ttcaaatcta
    10561 cctttggata agtatccggt gtaaacaaac cgccgtccgt cgccaaacct tgcaaaattg
    10621 cttggctggc agttactgta ttgttggcat cacgcgttga ttgataaact aatgtcatta
    10681 ctctctatcc tttatctata gtctcgtcca ttatatcatg atttttcaga aaattctccc
    10741 tttataagag aaattatagg ctcaattctt ctctcaaagg ctgtaaatag gtcatttctt
    10801 ccttatttct caccactagt ccctcctcag ctagtaggag taagtctttg gaaaattcaa
    10861 taatcgtagt ttcttcctca tctgtaagat ttttcttaga aaattgtctc cttaaagact
    10921 tgtaatcata accaaatact ttaaagaaag gtgctgtttc taagtaagct tctaacttgt
    10981 ctaaattaac caataatccc aagtgaaaag ctgcagaagc aaaagtccta tcaagtggct
    11041 gtgtacacac actacgaaac tcaactgttc ctcgagtcgt taaatcttgg tactggtaac
    11101 tacgatgagt ttcaaaatcc ttctcttggg ggtaaataat aacctcatcc ccattcagag
    11161 caaatgcttg gatttcggac gtagccaaat agtccccagc ctgaatagga taaaaataat
    11221 aggtctgccc atcacgttcc gcagtaaaaa tcgcagaatg atttagatag tcaaaaaaat
    11281 cagcttcatc attaaggagt ctagcattga ccccaacatt ctctggatag ataccatgca
    11341 tagattcttc ccagaaaata tcccttgaaa ttttcgtatc ccaatccgca cccgaaaatt
    11401 cagagtttgc aaataaataa gccttagccg cttcaatttg agtaaaagca ttaatcaccc
    11461 gtaagtagtt ggtttttgaa atatccagct gaacctggct cccacagata aaagtaccat
    11521 attcagggaa atgatgtaaa tctgatttaa taatatttct actcaaattc aaataatcca
    11581 tcaacatctg atagcgtgga taagccactg gacaattctc atttttatcc cagttgggat
    11641 ggataccaca gccaacaata gcatgatttg attcagctaa ctttctctga attacattca
    11701 tataattatt gaaacgattt tcgacctctt gaatcgtttc agccttacca aatgcaaact
    11761 caatcgttgt ataggaaact tcaaataaaa tagcatcctg acttatcgga tctactaact
    11821 ggatcagatt gccaaaatca tctacctttg ctacggtgag atccaaagta gaaactaaat
    11881 aatgaaatag atccttcata acttcaacat ctgtagcatc cccttctaaa cttgcaacag
    11941 gatactccaa ctcaattccg acaaacaatt caggattttc tttaatattt ttcaaatacc
    12001 gatgttttaa taaatcgata gaacaagaca tactccccta cactttctta atctaaataa
    12061 aatttgaata actataatta taccaaaaat aatataaaaa aggaacgaag aataacaacg
    12121 ttcctaacac tatactatac cggcggccgg ggtcgaaccg gcacgtcctt gcggacactg
    12181 gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatagtaa ctggggtagc
    12241 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    12301 caataatata aaataggcga gtgatgggga tcgaacccac gcatgccaga gccacaatct
    12361 ggtgtgttaa ccacttcacc acacccgcca taattctatt aacacgggca gtaggaattg
    12421 aacccacact gaaggttttg gagaccttag ttctaccttt aaactatgcc cgtaaaatgg
    12481 aaggggaggg attcgaaccc ccgaacccga aggagcggat ttacagtccg ccgcgtttag
    12541 cctcttcgct acccttccaa aatatataaa tggcgcgaga cggaatcgaa ccgccgacac
    12601 atggagcttc aatccattgc tctaccaact gagctaccga gccttattgc gggagcagga
    12661 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccgagctgct ccatcccgcg
    12721 ttaatataaa aggaggatgt gggattcgaa cccacgcacg cttttacacg cctgacggtt
    12781 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc aaaatagttt atacgggaac
    12841 aagcccgtgt cctcttagtg aacagataat attccaatcc cctagtaatg gactaaacac
    12901 tatgggcacg agtggactcg aaccaccgac ctcacgctta tcaggcgtgc gctctaacca
    12961 cctgagctac gcgcccaagt taaaaacttg gtacaaagaa caaagttcaa agcgggtgac
    13021 gagaatcgaa ctcgcgacaa cagcttggaa ggctgtagtt ttaccactaa actacacccg
    13081 ctaaa
//
LOCUS       DAVLFB010000051        12741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLFB010000051 DAVLFB010000000
VERSION     DAVLFB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.051"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            379..672
                     /locus_tag="VXD58_001307"
                     /pseudo
     CDS             379..672
                     /locus_tag="VXD58_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            779..1130
                     /locus_tag="VXD58_001308"
                     /pseudo
     CDS             779..1130
                     /locus_tag="VXD58_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1406..2712
                     /locus_tag="VXD58_001309"
                     /pseudo
     CDS             1406..2712
                     /locus_tag="VXD58_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2752..3762)
                     /gene="tsaD"
                     /locus_tag="VXD58_001310"
     CDS             complement(2752..3762)
                     /gene="tsaD"
                     /locus_tag="VXD58_001310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV0675997.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3752..4189)
                     /gene="rimI"
                     /locus_tag="VXD58_001311"
     CDS             complement(3752..4189)
                     /gene="rimI"
                     /locus_tag="VXD58_001311"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV0675998.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYVLAYDGAEVIGFLAVQENIFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4186..4869)
                     /gene="tsaB"
                     /locus_tag="VXD58_001312"
     CDS             complement(4186..4869)
                     /gene="tsaB"
                     /locus_tag="VXD58_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV0675999.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5047..5232
                     /gene="cibA"
                     /locus_tag="VXD58_001313"
     CDS             5047..5232
                     /gene="cibA"
                     /locus_tag="VXD58_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV0676000.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5234..5386
                     /gene="cibB"
                     /locus_tag="VXD58_001314"
     CDS             5234..5386
                     /gene="cibB"
                     /locus_tag="VXD58_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV0676001.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5709..5924)
                     /locus_tag="VXD58_001315"
     CDS             complement(5709..5924)
                     /locus_tag="VXD58_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676002.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            6204..6437
                     /locus_tag="VXD58_001316"
     CDS             6204..6437
                     /locus_tag="VXD58_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV0676003.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6439..8118
                     /locus_tag="VXD58_001317"
     CDS             6439..8118
                     /locus_tag="VXD58_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV0676004.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ASIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            8172..8264
                     /locus_tag="VXD58_001318"
                     /pseudo
     CDS             8172..8264
                     /locus_tag="VXD58_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8471..10384)
                     /gene="mnmG"
                     /locus_tag="VXD58_001319"
     CDS             complement(8471..10384)
                     /gene="mnmG"
                     /locus_tag="VXD58_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV0676005.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10394..10849)
                     /locus_tag="VXD58_001320"
     CDS             complement(10394..10849)
                     /locus_tag="VXD58_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0676006.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQYGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10990..12111)
                     /gene="mnmA"
                     /locus_tag="VXD58_001321"
     CDS             complement(10990..12111)
                     /gene="mnmA"
                     /locus_tag="VXD58_001321"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV0676007.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            complement(12511..>12741)
                     /locus_tag="VXD58_001322"
     CDS             complement(12511..>12741)
                     /locus_tag="VXD58_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676008.1"
                     /translation="WAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFK
                     VSDKWYYVNGLGALAVNTTVDGYTVNENGEWV"
BASE COUNT         3622 a         2973 c         2538 g         3608 t
ORIGIN      
        1 caattatatg cggatctata cctccacaat gtccattatt atacctaact ataatatgag
       61 ccgaaaacac tatatcctta atgtctccat atccatcagg gatattaata tttatttttc
      121 cacaactata ttgcattgta accatctcct taaacgacgc attatgatat ttgatagaga
      181 aatttttatg aataactcaa taattttata gtaaatcatg cttatatctc aaagatacct
      241 attttctctt gtctcgacct tctccaaaga attgctataa tactattaca aatacatctg
      301 cactacactt caaattttag cactgtataa aaacgtttca acacactaac tttaagaaaa
      361 cttccactat taattgaaaa agttgataga gataaattaa agatctatat tgaaactcat
      421 cccgatgctt atttgactga aatagctgct gaattcaact gtcctccaac aactattcat
      481 tacgctctaa aggctatggg atatagtcta aaaaagagct gtacctactg cgaacaagac
      541 ccagaaaaag taaatcggtt ccttaaagaa ttgaatcact taagctacct gactcctatt
      601 tatatttatg agacaggggt tgagacctat ttttatctcg aatatgatcg agccttgagc
      661 aggcagttag tctctctgga agaagatata attatttgaa ttaagatcga gacaacgcac
      721 accagagatt gcgatactgt tatagaagta ctaatgccct tttttgtttc aatatactat
      781 ggctccgatg acctataaag atacgatgac gagtgacttt ttcgaagctt gcttccaaaa
      841 attcttacta cctactttag atacaccatc ccttatcatt atggacaatg caaggtttca
      901 cagaatgaac atgtgtaagg agcagggcat agactgttac cacttcctac ctattcaccc
      961 gagtataatc ccattgagaa aatatgggct tacatcaaaa acatctcaga ataatattgt
     1021 caaattacga tgcttttctt gaggcacttt tgtcctattc ttgtttcagc cgactatact
     1081 ccgttattgg gcagctacgg aacagtcgat gggacgatgg ggggacataa aaaaatcctc
     1141 cagttttgtt ttttataaca gtatactgga gaattgacaa tctcggtaga tacctcgtta
     1201 tagcgcggtt acttattagg cagttacaaa acaactgtga acagaaaaca ttccagagtc
     1261 agacaagact ttggaatgtt ttggctctat aatttctgta gtgggtaatc ccaccccagg
     1321 aattataggg tcgtttcttg tagaaaaaaa gccccatatg acctataatg aaaagcgtct
     1381 aaccaactca ttagaaaggg ttcatatgga acaacttaag aataccacag atttgctcgg
     1441 attgaaagac aaaaatatca aaatcttgtc tgttctgaaa taccaaaccc atctagtcgt
     1501 tcaggcaaag ttggattccc ccgctcctcc ttgtcctcat tgtcaaggga agatgatcaa
     1561 atacgacttc cagaaagcct ctaaaattcc gcttctcgac tgtcagggtt tacccacggt
     1621 actacatctc aaaaagcgcc gctttcagtg caagaattgc cttaaggtgg tcgtttctca
     1681 aacatccatt gtcaagaaaa attgccagat ttccaacatg gtgagacaaa aaatcgctca
     1741 gctcctcctt gaaaagcagt ctatgactga gattgcccac agattggcgg tctcaacttc
     1801 caccgtcatc cgaaaactga gggaatttaa gtttgaaacc gattggacca agttgccaaa
     1861 agttatgagt tgggatgagt atagcttcaa aaagagcaaa atgagcttca ttgcccaaga
     1921 ttttgagtcc aaatccatcc tcgcaatttt agacgggcga actcatgcgg tgattcgaaa
     1981 ccatttccaa cgctatcaga gagaggttcg ggagctggtc gaggtcatca ccatggacat
     2041 gtacagccct tattatcggc tcgctaagca actctttcca aaggcgaaga ttgttcttga
     2101 ccgcttccac attgtccaac atctgagccg agctatgaac cgagtacgaa tccaaatcat
     2161 gaaccaattt gaccgaaaat ccttggagta tcgggcgctc aagcgctttt ggaaccctcg
     2221 ctttttcgtt tctaggctcg ggctaaatca gtccactgga ctgatttact acaccaggat
     2281 agcttcaagc tctgtcagaa acgattctat cagcccacgt ttcgaatgca cttaacccat
     2341 cgggaagtac gagataagct gctttcttac tctgagggat tacaggttca ctacgaactc
     2401 tatcaactcc tgctctttca ttttcaagag aagaatgccg accatttctt tggattgatt
     2461 gagcaagaac tgccaacggt tcatccgctt tttcaaacgg tcttttggac ttttttaagg
     2521 gatagagata agattatcaa cgcacttaag ctgccttatt ccaacgctaa acttgaagcg
     2581 accaataatt tgattaaggt tatcaagcgc aaagcctttg gtttccggaa ctttaacaat
     2641 tttaaaaaac ggattttgat gactttgaac atcaaaaaag agagtacgaa tttcgtactc
     2701 tccagattgt agcttttcgc ctacccacta cacttgacaa agagccactc tttattccat
     2761 ggtatcaaag gcaagacttg gtttggcatt gaggtcccag cctgcgaagt tttctttgtt
     2821 ccactcgctg acgctggcat aggcaatcat acctgcattg tctccgcaga gtcgcagagg
     2881 ggggatgata accttgacat ctgtgatttc ggctgctagg cgttctctga gacctttatt
     2941 ggctgccaca ccacctgcca caactagggt tttaacagga tatttctcca aagccttctt
     3001 ggtttttgcc atgagaatgt ccataactgc tgcttggaag gaagcacaca aatcttctgt
     3061 agacaggctt tctccctttt gctcggcatt gtgatgaaga ttgataaagg cagatttcaa
     3121 acctgagaag gagaactcca gattatcttc cttaatcatg gcacggggga aatcataaat
     3181 atcctgcccc tgatgagcca gctcgtcaat ctcacgacct gcaggatagg tcaagcccat
     3241 gacacgaccg accttgtcat aagcctcccc aactgcatcg tctcgtgtct ccccaacaat
     3301 cttgtaatct cctgcctccg aaacatagac caactctgtg tgcccaccgc taaccaagag
     3361 ggctagcaag ggaaactcca aaggctccac actctgagct gccatgaggt gcccagccat
     3421 gtgattaaca ggaatcagtg gaagtccgtg agcccaagca aaggccttgg cagctgacaa
     3481 accaactagc aaggctccga ccaagcctgg tccgtaggta accgcaacag ctgtcacgtc
     3541 ctcttcggta atccctgctt ctgccaatgc ctcctcgata caggctgtaa tgacctcgac
     3601 atggtgacga ctggctactt cgggcactac gccaccaaaa cgtttgtgac tctcaatttg
     3661 actagcaatg acattggaca agagctcatc gtcgtttttc aagacggcga cactggtctc
     3721 atcacaggat gtctcaaatg ctaaaatata tctatccttc atctatttct ctcttcatga
     3781 taatggcgtc ctcgactggg tcatggtagt aggcctttcg ctcagcgatg accgccattt
     3841 tttctttctt gtaaaatgct tgcgctcgtt gatttgactt tctgacttcg aggaaaatct
     3901 ccttatctgt cggcaattga gcaaacaagg ctgacgcaat tccctgaccc tgataagctc
     3961 ctttaacagc gatttgcaag acttctgcct caaagatatt ttcctgaaca gctagaaaac
     4021 caatcacttc tgccccatca taagccagaa cataccaagt ctggtcttgg gacaggtctg
     4081 cttggatttg ctccagagtc caaggactga ctaggtaaac agctgccata acagcgtaga
     4141 tggcttgagc taggtcaggc tgttgttgaa ttcgcttgat ttctatcata ggcgtttaat
     4201 gtaggactca ccagactcgg tatggttctt gagccagttt tcctcagcct cgactcgttt
     4261 gaggtaattc ggcacaaaat catgcaagga gtctgcttcc ttgtcccaag ctaaaagagc
     4321 aagattggct gcattgggca atgtttcttt gtaatcagtc cttggcaagt gtttttgaat
     4381 ctgctcaaca aaggggccaa cttctccgac aaaggttacc tgactagtac ccttgacttt
     4441 ttctagcacc tcttcaaaag ataggtgcgc ttctgccatg acaggtttgg cattttcata
     4501 aaatcctgca taaacattat tgcgacgcgc atccatcaag gggacaaaca aaccttcttg
     4561 ttggtggggc accagagcca agagactcga cataccaacc aactcgatgt tcagggtgta
     4621 agctaaggtc ttagcagttg ctaccgcaat tcgcaagcct gtatagctac ccggcccttc
     4681 agctaccacg attcggtcca aatccttggg tgtccaatcc aaacttgcca tcaaaaaatc
     4741 gatggcaggc ataagagtaa tactgtgatt tttcttaata ttaatcgtcg tctcggcaag
     4801 aacctgctta tcctctaaaa tagccagaga aagagccttg ctggacgtat caaaagctaa
     4861 tactttcata acacattcct atctttttgt ctgcttacta ttatactaca aaagctggca
     4921 catgggaatt ttctttgccc ccagacaaga gtgccctcac ttaactaaaa ataatttaaa
     4981 aaaatgctca cttttccttt tcttttccga atataaaagt gaacaagaaa aaaggaggaa
     5041 agttcaatga caaattttga cattcttgac aatcaatttt tatccttatc tgaaaatgaa
     5101 ttatcagata ttgatggcgg tctcgctccc ttggttatct ttggagtagc agtatcttgg
     5161 aaggctattg caggtggaac agcacttata ggttctggtt tggcagctgg ttatttttta
     5221 ggaggagatt aatatgatga aagatttgaa caattatcgt gaaatttcta ataaggaatt
     5281 gcaagaaatc aagggtggct ttggtgtcgg tgttggtatc gctttattta tggcaggtta
     5341 taccattgga aaagaccttc gtaaaaagtt tggtaagtca tgctagataa gaaacacatt
     5401 tttagaagga taaattttat tgtcttcatc tcttacagtt tgctcagcat tctcaatgat
     5461 ttgaacatta ctaccatccc tttaccattc gatttatctg tttgtattgt tttattttta
     5521 tgcttcaact ctatttttga tcagaacaat gactcccata aaaataataa gctttgaaaa
     5581 ttccattgtc atgtcatgtt agaaaaatgc aaagaccacc tcatcttgat agatggggtg
     5641 gaattttcgt gtcgtaaatc tactatctct acatccccaa acaaaaaacc ccagcataag
     5701 cagggcatct aagcatttaa ttcaaagtaa aatacaaacc aaacgacata ggtcacgagg
     5761 aggagaaaaa gcgagtagag agtcacaaag gtcattttcc acaagaactt ggtttgtcgt
     5821 cgttccagtt tggcaaatag aagattcccc gcataaacgc aagcaacaaa aacaataaaa
     5881 gctaccaagc gagctccgat agcaaaagca aataagttat acatagggca acctccttga
     5941 cttaaaatct atatggaatt atgacaagca ataaatttca cttccgttat caacataata
     6001 cattttcttt attcttgcaa acgcttacca aagaaatcgt cccctaactt tctcgtttcc
     6061 gtcttttact aatttttcat tttgtggtat aattgaaata attgtaacga atcaaggtca
     6121 atctagacac aaaatggaat gaaatcaagc aaatatctgc taaaagtttg gaataagctg
     6181 acctgtaaat agaaaggaac tatatgattt acaaagtttt ttatcaagaa acaaaagaac
     6241 gtagcccacg ccgtgaaaca acacgcgcac tttacctaga catcgatgcc agctcagaac
     6301 ttgagggtcg catcactgct cgccaacttg tcgaagaaaa tcgcccagag tacaatatcg
     6361 aatatatcga actcttgtct gacaaattgc tcgattacga aaaagaaact ggcgccttcg
     6421 aaatcacgga gttctaatat ggcctatact cttaaacctg aagaagtcgg cgtttttgcc
     6481 atcggtggtc tgggagaaat cgggaaaaac acttacggaa ttgaatacca agacgagatt
     6541 atcatcgtcg atgctgggat taaattccca gaagatgact tgcttggtat cgactatgtc
     6601 attcctgact actcttacat cgtggacaat atcgaccgcg tcaaggctgt tttaatcaca
     6661 cacggacacg aggaccacat tggtgggatt ccgttcctac tcaagcaagc aaatgtccct
     6721 atctacgctg gaccgcttgc cttggcttcg atccgtggga aactcgaaga acacggcctc
     6781 ttgcgcaacg ccaaacttta cgaaatcaac cacaacaccg agttgacctt taaaaatctc
     6841 aaggcaactt tctttagaac gactcactct attccagagc ctttggggat tgtcattcat
     6901 actcctcaag ggaaaatcgt ctgtacgggt gactttaagt tcgactttac tccagttgga
     6961 gaacctgcgg acttgcatcg tatggctgcg cttggtgaag aaggcgtgct ctgtctcctg
     7021 tctgactcga caaatgcgga agtaccaacc tttaccaact ctgaaaaagt cgttggtcag
     7081 tccattatga agattatcca aggtattgaa ggacgtatca tctttgcatc ctttgcctca
     7141 aatatcttcc gtctccagca ggcaacagaa gctgctgtta agactggacg caagattgcg
     7201 gtctttggtc gttctatgga aaaggccatt gtcaacggaa tcgatcttgg ctacatcaaa
     7261 gctcctaagg gaacctttat cgagccaaat gaaatcaaag attatcctgc aggagaagtt
     7321 cttatcctct gtacaggtag tcagggtgag cctatggcag ctctctctcg tatcgccaac
     7381 ggaacccacc gtcaagtaca attacaacca ggtgataccg ttatcttctc ttctagtccc
     7441 atccctggaa acactactag cgtcaacaag ctgattaaca tcatttctga agctggtgtc
     7501 gaagttatcc acggtaaagt gaacaatatc catacatctg gacacggtgg tcagcaagag
     7561 caaaaactca tgctctgctt gattaagcca aaatacttca tgcctgtcca cggtgaatac
     7621 cgcatgcaaa aagtccacgc tggactagca gtggatactg gtgttgagaa ggacaatatc
     7681 tttatcatga gcaatggcga tgtgcttgcc cttactgctg actcagctcg tatcgcaggt
     7741 catttcaacg cccaagatat ctatgtcgat ggaaatcgta tcggtgaaat tggcgcagct
     7801 gtcctcaaag atcgtcgcga tctatctgaa gacggtgtcg ttctggcagt cgcaactgtt
     7861 gacttcaaat cgcagatgat tctgtctggt ccagacatcc tcagccgagg ctttgtctac
     7921 atgagagagt ctggcgactt gattcgccaa agccagcgta tcctcttcaa tgccattcgt
     7981 atcgcactga aaaataagga tgccagcgtg caatctgtca atggtgccat tgtcaacgct
     8041 attcgcccct tcctctatga aaataccgaa cgtgaaccga tcatcatccc gatgatcctc
     8101 acaccagatg aagaataaag caagaaaaca gccccgtcct cggagctgtt tttctctatg
     8161 ctttcttttg agattaaaac tcatactcaa tgaaaatcaa agagcaaact aggaagctag
     8221 ccgtaggttg ctcaaagcac tgctttgagg ttgtagatag aactgacgaa gtcagtagcc
     8281 atacctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tatcaataaa
     8341 aatcgaaatc agactagaag gctaagcgaa agcataactt gagttagctc ccatagttcg
     8401 ggaaactatg ggaggctgga gatgaatcaa agccaagctt tgaactcatt cgtaagaagc
     8461 cgacgacgta tcattttgat ttttgaagag ttttagaaat actacgattt ttaccttcca
     8521 gatacaccat caaaatagaa atatctgctg ggtttactcc cgaaatacgg ctggcttggc
     8581 cgatggtttc tggattgatg agtttgaact tctgacgggc ttcggttgcg atagaatcaa
     8641 tgtcatccca gtcgatattg gccggaatgc gtttttcttc catgcgtttc atcttggcaa
     8701 cctggtccat ggctttggaa atatagcctt catacttgat ttctgtttca atcaattcga
     8761 taatcttgtc atccaagtct tctgcagctg gtccgatgaa ggccaccaca tcttggtaag
     8821 aaacttctgg acggcgaagg aattccttgg ctgtcactgc atcggtcaag ggtttgaagc
     8881 ccatctcctc aaccttggca ttggtttcct tgactggctt aagtttgata ctgtctaggc
     8941 gcttcatctc attatcaaat tgatttttct tgatttcaaa acgagcccag cgttcatcgt
     9001 ccacaaggcc aatctcgcgt cccatctcag tcaagcgcat atcagcattg tcatgacgaa
     9061 gaatgagacg gtattcagca cgactggtca agagacggta gggttcaatg gttcccttgg
     9121 tcaccaagtc gtcgatcatc accccgatat aaccatcact gcgcttcaaa atcaattcag
     9181 gcttgccttg gattttcaga gccgcattga tacccgcgat aatcccttgg ccagcagctt
     9241 cttcgtaacc tgatgttcca tttgtctgac cagcagtgaa gagacctgag attttcttgg
     9301 tttccaaagt cgcacgcaac tgatgaggca agaccatatc atactcaata gcataacctg
     9361 tccgcatcat ctctgcattt tccaaacctt tgatggaatg aaccaagtca cgctggacat
     9421 cctcaggcag actggttgaa agtccttgaa catagacttc ctcagtattg cgcccttctg
     9481 gctcaaggaa gagttggtga cgttccttgt ccgcaaagcg cacaatcttg tcttcaatcg
     9541 acggacagta acgaggcccc actcccttga ccacacctgt aaacataggc gcacggtgga
     9601 ggttgttttg aataatctca tgactggtac cattggtata ggtcaaccag catggcactt
     9661 ggtccttgac ataatcctca tcacgtgaag tgtatgagaa gtgattaggc gcttcatctc
     9721 ctggttggat ttctgtcacg tcgtagttga tagaagaggc cttgacgcgt ggaggggttc
     9781 ctgtcttgaa acgaccgatt tcgagaccca gttccttgag attgtcagct aggttaatag
     9841 aagccaagct gtggttagga cctgatgagt acttgaggtc tccgatgata atttccccac
     9901 ggagagcagt ccctgtcgtc acaataacag ccttagcagc atattcttga tgggtggctg
     9961 tacgcacacc gacaaccttg ccatcttcca ccaaaatctc atcaatcatg gtttgacgaa
    10021 gggtcagatt ttcttggttt tcaaccgtct tgcgcatctc cttagagtaa agttccttgt
    10081 cagcctgcgc acgaagggca cggacagctg gccccttccc tgtgtttagc atcttcatct
    10141 ggatgtaagt cttgtcaatg gttttggcca tctcgccacc gagggcatcg acttcacgca
    10201 cgacaatccc cttggcagaa ccaccgatag agggattaca aggcatgaaa gccagcattt
    10261 caatattgat ggtcgcaagc aggaccttac agcccatacg gctagcggcc aaggaagcct
    10321 caaccccagc gtgtcccgca ccaattacaa taatatcgta ttcttcagta aaatgataag
    10381 tcatgtttct ctcctattcc tcaagatgaa tgtgtcttag ttggccttcc caatctggta
    10441 gggctgtttt taaaaaggct ggaactagct ggatattctg gagcttatcc aagtcaatcc
    10501 actcacaggg ctgccttttc tcatcttcct gcatggtcaa tggggcatct tcaagcaaat
    10561 ccaccagata atgaaactcg atattgtgat aggaaacacc gtattgttca aaacgatttt
    10621 caaccacaaa agctagctgc ccagcttgag ctttaacacc cagttcttcc ttcacttcac
    10681 ggactaccgc gtcttccgtg ctttcattga cttgaatcgc acctccaata gtgtaatact
    10741 tgcccttgtc tttggtaact agaagcttgt gattttggac aatcaaggct gtcgcccgaa
    10801 caccaaaaac cgtattgtct acttttgtcc gaaagtcttg ttgagtcatt cttgtccttt
    10861 cccttaaacg acacaaaaac agtcaaaact acaaagaagt gcaggacaaa aaagcctgca
    10921 acatccatga gctttgacca tcatttctat tgctgttatt attgtagcaa attgagaaaa
    10981 tttgtcaatc taaatgtact gacaaacttg tccatcgcgg taagcattgt caatcaaacc
    11041 accacctaga cactcttcgc catcgtaaaa gacaactgcc tgtcctggtg taatcgcgcg
    11101 ttgtggttcc gcaaagatga cctctgcctt gtctcctttg acatggacgg tcaccttaga
    11161 gtcaggctga cggtaacgga atttagccgt acattctagc gtaaactctt ctggcatttc
    11221 acgagtaaag tggacttgac tggcttctag gctagttgac atgagcgaat catggtagaa
    11281 tccttgtcct acatagagaa tattcttgct tagatctttt ccgacaacga accaaggggc
    11341 attgtcaccg ccgtgttgcc caccgatacc gagtccgcca cgctgaccga ttgtatagta
    11401 cataagacct gcatgctcgc ccatatcgcg accatccaca gtcatcatgc gaccaggctg
    11461 agctggcagg tagttgctga ggaagttttt aaagttcttt tctccgataa agcaaatccc
    11521 tgtcgagtct ttcttcttag cagtcgaaag gcctgcttct tctgctagtt ttcgaacttc
    11581 aggcttttcc aaatgtccta gtgggaacat ggttttttga agttgttctt gcgaaagttg
    11641 gctgaggaaa taggtctggt ccttgccatt gtccacgcca cgaagcatgt gaacagtgcc
    11701 gtcctcatca cgcgccactc gagcataatg cccagtcgct acatagtctg cccccaaggt
    11761 tatggcatag tccaaaaagg ccttgaactt gatttccttg ttgcacataa cgtccggatt
    11821 tggcgtgcgc cctgcacggt attccgctag gaaatactca aaaacgcggt cccagtactc
    11881 tttttcaaaa ttgacagagt agtagggaat gccaatctgg tctgccaccg caaccacatc
    11941 cttgtaatct tcggtcgccg tacagacgcc gttttcatct gtgtcatccc agttcttcat
    12001 gaagataccg atcacatcgt agccctgctc cttgagcaaa agagccgtca ccgacgaatc
    12061 aacaccacca ctcatcccca cgacaacacg tgttttagag ttatcactca tggtaagtct
    12121 cccatctatt cgtttattca cgattgaagg tcgtgtgtgc ttcacgattg aaggtcgctt
    12181 gagcagtatt cattataaca cgcttggtta gagaagacaa ggaagagctt aaaaattgtt
    12241 ttttaaaact tatagtcaat atacagatta tgtgaatgac gtgagtatac cttcttctta
    12301 tcttcattca aataactata ataaataaaa caacccattt tctctaatct atttttcaag
    12361 ttagagaaaa ggagtcgttt tttaatgaca tttcctgaaa tcataagaca ggtacctaaa
    12421 gaatacgaac ataaatctat tcaatcgaac cttatctttg tttcaaaatt aaaatgtcaa
    12481 atgttcttaa catgatttaa tttaatcggc ttaaacccat tcaccgtttt catttacagt
    12541 atatccgtct acagttgtgt tgactgcaag ggcacctaag ccattgacat agtaccattt
    12601 atctgatact ttgaaccatt ggcttgcttt catagcacct gatgcttcaa gatagtacca
    12661 ggtatctcca tctttcaccc aacctgttgc cattgaaccg ttagcgttta ggtagtacca
    12721 tgaaccgttg actttagccc a
//
LOCUS       DAVLFB010000052        12680 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLFB010000052 DAVLFB010000000
VERSION     DAVLFB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.052"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(348..1514)
                     /locus_tag="VXD58_001323"
     CDS             complement(348..1514)
                     /locus_tag="VXD58_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0676009.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(1527..2351)
                     /locus_tag="VXD58_001324"
     CDS             complement(1527..2351)
                     /locus_tag="VXD58_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV0676010.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            complement(2486..3700)
                     /gene="thiI"
                     /locus_tag="VXD58_001325"
     CDS             complement(2486..3700)
                     /gene="thiI"
                     /locus_tag="VXD58_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV0676011.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(3709..4851)
                     /locus_tag="VXD58_001326"
     CDS             complement(3709..4851)
                     /locus_tag="VXD58_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0676012.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            4989..5417
                     /locus_tag="VXD58_001327"
     CDS             4989..5417
                     /locus_tag="VXD58_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676013.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(5568..7793)
                     /locus_tag="VXD58_001328"
     CDS             complement(5568..7793)
                     /locus_tag="VXD58_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV0676014.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7957..9909)
                     /locus_tag="VXD58_001329"
     CDS             complement(7957..9909)
                     /locus_tag="VXD58_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0676015.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(9906..10817)
                     /gene="pfkB"
                     /locus_tag="VXD58_001330"
     CDS             complement(9906..10817)
                     /gene="pfkB"
                     /locus_tag="VXD58_001330"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV0676016.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(10814..11554)
                     /locus_tag="VXD58_001331"
     CDS             complement(10814..11554)
                     /locus_tag="VXD58_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0676017.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            11717..11804
                     /locus_tag="VXD58_001332"
     tRNA            11717..11804
                     /locus_tag="VXD58_001332"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11751..11753,aa:Ser,seq:gga)
     gene            complement(11987..12334)
                     /gene="ssrA"
                     /locus_tag="VXD58_001333"
     tmRNA           complement(11987..12334)
                     /gene="ssrA"
                     /locus_tag="VXD58_001333"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3718 a         2842 c         2422 g         3698 t
ORIGIN      
        1 tactgactct gtcagttcta tccacaacct caaaacagtg ttttgagcaa cctgcggcta
       61 gcttcctagt ttgctctttg attttcattg agtattaata gactggtttg gtatcctgaa
      121 aactatatcg tataggtgtc gtcatggatc cttttagctg atttaaggat acgaaaacac
      181 aatactatga ctaaattgaa atgttaaaat tcaatttcta gccacctaat cctgcttcaa
      241 atagttatcg aacaagtttt atgatagcta ccttgttagg atgctcttat tttttaccag
      301 acaaaacaaa aggattaagg gagtgaattt ccttaatccg atagttctta ttcttgacgt
      361 tgtccgaaat cagcaatcat ctgatccatt tcttgtcgac tcggagttgt cagcatatac
      421 aggtaatctc cttgaagttc cgcgaaggca gctggcatga cttttttaac cttgaactgc
      481 tggctatatt tggcaagcaa atcctcttct gaataaacat agtgagcatt tgcacggcga
      541 gaagtagcca aacaatactg agtttcaaag tctgacgccg ggaactcctc tcctgcgaca
      601 atagctgcat agccacgata aaggtccaag gaatgagcaa agttataaac atcaatggta
      661 aaaccacctg cagggcggtt attgtactcg atggtaatat aatcgtcccc ctcacggaag
      721 aactcaatat ggaaaaaccg ttctttcata ccaaattctt tgacaattgc ttccccatac
      781 ttgcgcagtt taggatccat atccttgagc acataataag aattgtccat cttataaatc
      841 atgaggtcaa gcggtgtata ggcgtagtca aaggttgttg agaagacaat ctttccatcc
      901 ttgtccacga gcccgtcaaa ggtacagatt tcgctggaag tgacaaattt ttcaaagaaa
      961 taaagggttg aatggtccca ttcttgcttg aagtgattga tatcgtcttc tgtctcaagt
     1021 ttaaaggttg cggctgctcc cactccatta tcaggtttgg caatcattgg aagaccgatt
     1081 tctttcactg cttgatcaac atctgcttcc gtcttgataa cagctccagg taccacagga
     1141 acacctgctt ttttgaaaag tttcttcatt tcagacttat atttcgtctt tttgagatcc
     1201 tctggtttgg caccaaaaac attgaattgt tctctgagtg ttgcgtctag ctcaagccag
     1261 tattcattgt gagactcgat gcggtcaatt ggaccatgtt tataaaagag aaaagcaact
     1321 gcacgtttga cttcatctat gttctcaaga ttatcaacac gaaaatactc ggtcaagcta
     1381 ttgcgcaagg gctcatccaa ttgctcgtaa gactcttgac caattcccaa gactgtgatg
     1441 cctttattag ctagttcgat ggtaaactgt tgaaagtttt gtggatagta gggagaaata
     1501 acaaggtaat tcataggtaa ctccttttat aaatagagat taccgaggaa ataaggcatt
     1561 tgtttacgcc accattccca gtcgtgggcg acatcatgtc cccattcagc aaaccaggct
     1621 ggaatttgtt tctggtcaaa ggcttctttg agcttgtaaa aggatggtaa accatcttgt
     1681 tcccaggctc caagccccgt acacagcaca atctctgcct gacggtaacg gtcaataaac
     1741 cagccatcgt tttggttcca aatataatct actggcgagt tttggtaaat agcatcatcg
     1801 ttgtagtaat cgccgacaaa gaaacgtgcg tcgtaaacac cactgagagc aatcactttg
     1861 gtaaagacat ctggatgctg gaggaagaaa ttgagtgcat gataggctcc catagagcaa
     1921 cctgtcgtca tcatgccatc aaaccaacct gttttgtgct tgataaaagg cttgaccacc
     1981 tccatgacaa actcagcata ctccacaccc ttaccaccaa actgctgctc tgggatagga
     2041 gattcttgga acttccaagc cgcatactca ttcatccgcc ccataccatc attgtcaata
     2101 gcaacgacaa tcatgcgact gatatccgga tttcgtttga tagctgggat aatcttccat
     2161 gaatgtccaa tgaaagactc tttgctatta aaaacatttt gcccgtcatg aaagtataca
     2221 acaggatagg aacggtctgt atctttctca taatctttag gaagaagaat acgtacacgg
     2281 cgctccttac ctgtataagg aaccttgagt ttgtgttctt tcatttttag ataaaagtag
     2341 gattgattca ttgtcagaaa actctctata ttcaaaattt tatatcatta tatcataaaa
     2401 ataaaaaaag ttagtttttt acctgttttt agagaaaaaa ctaacttttt ttactgattt
     2461 tcgctattct tttggatttt ctgaattaga gcagattgtc aatcaagtca tcaacttcat
     2521 ctttttcggc ttgaggtgtg atttcagtaa tcatgattcc agccactgct cgctcaacca
     2581 agccttcaac atccatacgc gcttcgtact gctccgcatt cttaatttta ggatttgttt
     2641 ttggacgatc tggtgcaaaa atagtacaac agtcttcaaa cggttggatt gaaatgtcaa
     2701 aggtatcgat ttcctgggcg atgtcaatga tttccaactt gtccatggta accacaggac
     2761 gaatgatggg agtgttggta acagcattga tagccttcat actttcaagg gtttggctgg
     2821 ctacttgacc tagactttcc ccattgatga taactaaacc atttcgtacc tcacgaatac
     2881 ggtcagtaat acgcatcata aaacgacgcg tcaaggtcat aaggtaggct tctggcgctt
     2941 tggctttgat ttcctcttga atctctgtaa aaggaacttc gataaactgg atatttcccc
     3001 caaatttggt taatttacgg gtcaaatctt gggctttttt aagagcacca ggactcgtgt
     3061 aaggtgggct ggcaaagtga actgcctcta tatctacccc tcgtttaagc gctagatagc
     3121 cagctacagg ggaatcgatt ccccctgaca acatgagcat gcccttacca gaagttccca
     3181 ctggtaatcc acctgcccct cgaacggttt cataagaaag ataggctgct tcttcacgta
     3241 tctccacctg aagattgatg tcaggacttt tcatttgagc ttgtacgttt ggaatggctt
     3301 cgaatacagc ccctccaagc gtttggttga gttcacgact gtccaattca aaggtgtggt
     3361 cgctacgctt gctagagatt ttaaaggtca tgccttcctt gtagatgtcc tgcataatct
     3421 cttggacagc agacttcaga acttctacag atttttcaac cttataaaca ggggaaaagt
     3481 tttgaattcc aaatacttgt ttgagcgatt ctgctactgc tgtgtaatct gctccattga
     3541 ggtaagcgtg ggcacggtcg cgatctgctg ttaccttaac ttggggatag atagacaaaa
     3601 cgtctgaaat attattgcga agtttattga tgaaacgcat acgatttttg cccttggttg
     3661 acagctcacc gtagcgaatc ataatttctg aatactgcat gaatgctcct atcttacttt
     3721 tctagtttga ttgtaaatta attttagctt ggtcaagaac tgctcgacct gactcatatc
     3781 attttcaagg tctagactaa gacgcacagc tgactgagcc ttatccttgt ccactcccat
     3841 agcaatcagg gttccggctg gtttcccagc cttggacgaa caagccgagg tcgttgagat
     3901 gaaaatatca tagtcttcaa aggcgtgaac aatgacctca ccacgaacac ccttgattcc
     3961 aaaagtcaga atatgagggg cgaagtcttc ctcatctgaa aagacaaata tatccggata
     4021 gtccagaagt gcttgacgaa tcactgcctt catctgccca gtcttgctcc taaagatatc
     4081 tagcttttcc atagataaac ggagagcctt agcggtcgct gcaattcctg ccacattttc
     4141 agttgtcgaa cggtaatcac gttcctgacc accacctgtt aaaagaggcg taatcttctt
     4201 gccagacttg atatagataa aaccaacacc acggattccg tggaacttgt gaccagagaa
     4261 agtcgcgaaa tccactcgtt ctgtcagata cttttcagtc ggaatcttgg caagcgcctg
     4321 aaccgcatca acgtggaagg aaatagttgg cttgtctgcc aagaattctg aaatagcctc
     4381 aataggttgg atagagccga tttcattgtt cacagccatg atggaaacga gggtcgtatc
     4441 aggtcttatc aaatcttcta gcgcctcaac atccacaaat cctttgttat caactggagc
     4501 aaaatccact tcaaaacctt gagctttcaa ccagagggct gattccttaa ctgctggatg
     4561 ttcaatggct gatacgatga tgtgcttgcc aaactgggct ttttcaaatg ctactccctt
     4621 gatgatccag ttatcccctt ctgtcccacc agatgtaaag aagatttcat cacttttctt
     4681 accgattaaa tctgcaatct gttgacggga agcatctaaa attcgtgttg cctggtctcc
     4741 caaacgatgg agactagatg gatttcctaa aatttttgaa gccacctgca tataggtttc
     4801 aagggcttct ggatagggcc tggtcgtcgc cgaattatca aagtaaatca tgtttctcac
     4861 gctttctaaa atcactcctt ctattgtatc atgaaacgga gcctgcgaca agaaagggca
     4921 atttctcttt ttttaagatt tttttaaaga aacagagtat aataaatcat aatcaaagga
     4981 gagaatgtat gtctaactat cgtagaactt caaaaccgaa aacagaacat atcaaaaaag
     5041 gctttactgt ttttcaaaaa accattacta ctatcggtag tatccttggt ttgattaccg
     5101 cgggtatcac gatcatgaac gccttggata ataataataa aaatactaaa aaagaaccta
     5161 cgacaagcca gacgacaacc tttgtcaaag aaattcaaaa agaatcccct caggaaaata
     5221 ctactccaaa taaggaaaat aacacttctc aagaaaaaac acaacaagaa gaaacgccaa
     5281 aatctagcgt caaggaagag aaaaaagaag atcagaaaac agcaactcag gactctacta
     5341 cacctgctac aagtaaacct gccactgaaa atgaaaaaca gcccaatact ccaacttcag
     5401 aaaataatac tcaatgaaaa tcaaagagca aactaggaag ctagccgtag acagtacttg
     5461 agtacggcaa ggcgaagctg acgtggtttg aagagatttg cgaagagtat aaaagtaatc
     5521 aatagccagt aaaatagctc cttccaacct tggaaagaag ctatttttta ttgctgcaat
     5581 acttttcttg gcttggtacc ttcagctgga ccaatgacac ctgccatctc aagctcttcc
     5641 atgagacggg tcgcacggtt aaatccaact gacaaacgac gctgaatcat ggatgcactg
     5701 gctttctgtg tttcgataac caaagactta gcttcttcaa aaagcggatc accaccagca
     5761 tctccatccg aaaattctcc ttcattttca gaaacctcac ctggatcaaa actctcatcg
     5821 tagtctgcat ctgcctgagt cttgatgaag ttcacaatgc gctcaacatc gtcatccgag
     5881 ataaaggagc cttggagacg aactggatga ttttcatcaa tcggtttaaa gagcatgtct
     5941 cctcgaccaa gaagtttttc tgctccattt tcatccaaaa tcgtacggga gtctgttcct
     6001 gatgaaaccg caaatgctac acgagatgga acattggcct taatcaaacc agagatgaca
     6061 tcaacagatg gacgctgagt tgcaagaatc atgtggatac ctgcagcacg cgccttctgc
     6121 ccaagacgga tgatagcatc ttccacttcc ttgctggcca ccatcatgag gtcagccaac
     6181 tcatccacaa tcacgacaat gaatggtagc ggaatttgct tgtactcaga ctgggaattg
     6241 aactcttcta ccttggcatt aaaacctgca atattccgaa ctcccacctt ggcaaagagt
     6301 tcataacggt tttccatttc atccacaacc ttttgcagag ccttgctggc tttgcgtgga
     6361 ttggtcacga ctggaatcaa gaggtgggga atatcattgt aaacagataa ctcaaccatc
     6421 ttgggatcga ccatcataaa tttaacttga tctggtctcg ccttcatgag aatgctagca
     6481 ataatgccgt taactgctac tgacttccct gaacccgttg aacctgcaac tagcaagtgg
     6541 ggcattttag aaaggtcaaa agctcttgcg gttccattaa cagccttccc taaaggaatt
     6601 tccaagaaat tttctgcttt cgtttgcgat tgttcccata gttctcggaa agatacagtg
     6661 gcaatatcgg agttgggcac ttcaattccg attagggatt tcccagggat tggtgcttca
     6721 atccggacat ctttggcagc caaggctaga gcgaggtcat ctgatagatt ggaaatgcgg
     6781 ttgaccctta caccaacagc cggcttgact tcatacttgg tcactgatgg cccaatttcg
     6841 gcccgttcaa ctgttacctt aataccaaag ctagcaaagg ttgcttctaa gattttgata
     6901 ttttctctga caattttctt ctctttagac tgatcttttg gtttatctgg tgcaaagagt
     6961 tgtaagcttg gaagtttgta ttcaagggct tctttggctg aaaaatcgac ctgaacatct
     7021 tcgtcatctg agtcatcttc ctgttcaggg aatttaagtt cagcttgagg caggatgatt
     7081 tctggttcca cccacttttc ttctggaata ggtggaagat tttgaacagc ttcctctgtc
     7141 agaatttcac ccgtttccat atcaacagga ggcaaatcga gtaaggcttt ttcagtctct
     7201 tcttgttcta atctagcctc tttctcagcc ttttggcgag ctttttcttc ttgtttgaca
     7261 aagcgttcct cttttcgacg ctcgtgccct tcccaccatt tggcaaagcc tctactgaaa
     7321 aattcagcaa tatcgtaaac agaccaaggg ctgactagga gagaacccac taaaatcaag
     7381 atagaaccaa taaagtaagt tccgatattt gaaaagagaa aagctgttgg aatataaaga
     7441 gcgaccccga tcaagccccc tccagcaaaa ctagtcgttc gaaaaccagt cagatctgtc
     7501 acaacctgag ccatggtccc ttttagaacg gacttgtcca aaccatattt ccaaaccaag
     7561 taggcttcaa aaatcaagag taagccagca aatatggtga aaaagccaga taagagtcct
     7621 tcctgttttc gtatccactt gaaaaaaaag agatagatta ataggccgaa tatcgccaga
     7681 taagctaggc tacccactag caagcgaatt aaattataaa gggttatacc tgcagcccct
     7741 aatttgaagg ctgcgaaaat caataaaatc gcaattccta acgaaatcaa cattcgttga
     7801 atcgcttctt ttctttccag ttctgcttta gacggtctcc gtcttgttgt acttgtattc
     7861 ttgtttgcca ttcttctatt ataccatatt tcacaggcat ttcggaaaga gaaaaagact
     7921 gcaccaaacg gtccaatctt ttcatttttc tattttttat gcttgtggtt tgcgtaggta
     7981 accataaacc acaccactta cgattgctcc taccaagaca gaaacgaggt aaaggagagc
     8041 atttgaagta agggcgataa cgaagattcc tccgtgtggc gccatgagtt taataccagt
     8101 aagaccaacg agtccacctg ctactgctga accaaggatg aagcttggaa tcgcacgagc
     8161 tgggtcagcg gcaccaaatg gaatcgctcc ctcagtgata aatgacaagc ccatgatgat
     8221 gtttgtcaaa ccagagttac gttcttcctt agtaaattta tctttgaaaa gaagagttgc
     8281 gacaaagatt gcaagtggtg gcaccattcc tccagccata actgctgcca tggctacaga
     8341 accacctgaa gaaacagttg ctgcaagcgt acctgtacca aagacataag ctgctttatt
     8401 aactggtcca cccatgtcaa cagccatcat tccaccaagg acgataccaa gaaggacagc
     8461 tgaacctcct ccaagaccgc ctaggaagtc attcatagca gtgttgattg ctgccattgg
     8521 gatattcaca gctagcataa caaatcctgt caagattgtt ccaagaagtg gcaatagaag
     8581 gattgattta gcaccttcga gtgaacgagg aactttaacg tatttcttga tggcaagaac
     8641 caaggcacct gcgataaatc caccaacaag ggcacctagg aaaccagatg agacacctgc
     8701 aagagttgaa gttgcttcac cacctgcggc ataaggaatt ttaccaaagg caaaaccttc
     8761 tttggcaata gcaccagcca cgaaacctgc taccaaacct ggtttttcag caatagagta
     8821 ggcaacataa cccgcaaaga ctggaagcat caaaccaaag gcagctccac caattttcat
     8881 gaacatagaa gctaactcat ggtaagaacc aagattgcca aggttttcat ttggaacacc
     8941 caaagcaccg tcaatcaaga aggcaagggc aatcatgata ccaccaccga taacgaatgg
     9001 taacatttga gatacaccac tcatcaagtg tttgtagaag gcaccaccaa ggctttgttt
     9061 ttcgttagag gctgttgcag cttttgcacc attagcggca cggtagactt cagtatctcc
     9121 tgaaagagcc aagttaatta gctcttctgt cttacggata ccgtcagcaa ctggacgatt
     9181 gatcaatggt tttccatcaa atcgatccat ttcaacggcc ttgtctgctg caatgataat
     9241 agctttagcc ttacggatat cttctgcagt tagttgattt ccaacaccgc tagcaccgtt
     9301 ggtttcgacc ttgataccaa cccccatttc agcagctact ttttgaaggg cttcttgggc
     9361 catgtaagtg tgggcaatac ctgttgtaca agctgtaaca gctacgataa agtcaccaga
     9421 gtcattagca ggtacttgaa caagttcctc agttttttct gaagcttggt caaaaagttc
     9481 gataacttgg tctgcagatg ttgcttgacg aagtttgtct gcaaaaccgt ctttcatcaa
     9541 gtattgagac aattctgcca aggctgccaa gtgagtatca ttggcacctt ctggagctgc
     9601 aatcatgaag aagaggtcag ttgcttgtcc atccaagctc tcgtagtcaa cacccttatt
     9661 tgacttagca aatagaactg tcgcttcttt gacagcagcg tttttgctgt gaggcattgc
     9721 gattccatca cccaaaccag tagaagtcaa agcttcacgc gccaaaattc cttctttaaa
     9781 tgtttcaaaa tctgttacat aactgtgctc tgtcaaattt ttaatcatct cgtcgatgac
     9841 agctgttttt tcagttgcct gcaaatctag caacatgaca tcttttctca ataggtcttg
     9901 aattttcatc ttttttctac ctcaactttt ccatatgttt ctttaataaa ttccgccgtt
     9961 gccaagtcat ctgagaaggt agttgccgtt ccgcaagcca ctccccattt gaaggcttct
    10021 actgcgtctt ttgatttgac aaattcacct gtgaatccag caaccataga atcaccagct
    10081 ccaactgaat ttttgactgt tcctttgatt ggtttagcga agtaagctcc ctcagatgtg
    10141 acaagaaggg cgccatcacc agccatagag ataataacat tttgagcacc cttagccagt
    10201 aactcacgag cgtatttctc aatttcatct aaactttcga gtttaacccc aaaaatcgct
    10261 ccaagttcat gattgtttgg ttttacaaga agaggctggt agtccaaact atcaattaag
    10321 gtctgtcctt caaagtcaca gaccacttgc gcaccagtct ggcgcgtcaa ggaaatcaaa
    10381 tccttataga taacattgcc tagattttta gcacttgaac ctgcaaagac aactgtatct
    10441 tctgctgtca gactagataa aatagctttc aattcttcta gctgaaccgg ttcaacagtt
    10501 ggacccgttc cgttgatttc tgtttcttgg tctgctttga ttttaacatt gatacgagta
    10561 tcttctgcca cctggacaaa acgtgtctcg atttcttcct ctgctaaagt atctgtgata
    10621 aatttaccag taaagccacc gataaatccc gtcgctgtat ttggtatatt caaacgtttc
    10681 aagacacggc tgacattgat tcctttccca ccagcaaact tatcatcact gtccatacga
    10741 tttacactac caactttgac ttggtccaaa cgaacgatat agtcaatgga tggattgagt
    10801 gtgactgtat aaatcatact tctattacct ccgttttctc cttaataacc tgcaagagct
    10861 catgcccttg actagtgata acgatagcgc gtttgagtgg tgctaccttg gcaaagcaag
    10921 tttgtccaat ttttgacgaa tccaccaaga cgtaggtctg cttggcattc tccaaaatag
    10981 ctcttttcac agctccttcc tccatatcag gagtcgtata atagccatcg tcaacaccat
    11041 tcattccgat aaaggcacgg tcaaagtgca attggttaat ctggttaaga gcaacgcccc
    11101 cgatactagc atctgtcgcc gtcttgacgt ttcctccaac catgacagtt ggaatctgct
    11161 tttcaaccaa ctgagcggca tggtgaatgg agttggtcac aactgtaaca ttcttattga
    11221 ccaattcatg aatcaaaaaa gcagttgttg ttccagcatc gataaagatg acatcttttt
    11281 ctttaatgag agaggctgct ttctgagcca gcaacttctt ttcttgaagg tttttgacag
    11341 atttttcttg aatggtttct tcttcctgta aggagtgggg gagttctgct ccaccatgca
    11401 cacgacgaag cttgttttcc gcttccaact catccaagtc tcttcgaacc gttgattctg
    11461 acgtttctag caaactaact aatttttcta gagaaactac atgatgttga tttaactcct
    11521 ctaaaattag ttgtttacgt tctgttttta acactgaatc acctcctgtt atcgtttaca
    11581 ctattcattc tatcatactt tctttcgaag tcaagcattt tttatcattt tctttcaaaa
    11641 tatatcattt tttcttattt ctagaatttt tattgcaaga taagagcctt tatggtagac
    11701 tatttgagta agtattggaa gattactcaa gaggcttaag aggccgtgtt ggaaacgcgg
    11761 taggcgtgta atagcgtgcg tgggttcgaa tcccatgtct tccgtcgaaa aaagattatt
    11821 tctaggatgc gacaaatgtt gtatcctttt ttctatcctt tttttaaaat agggtctgtt
    11881 tgtgactccc tttttagcaa aagaatgcaa aggaatacca aagataaaaa taaaaaacgt
    11941 tgatttaaca acgttttacc aaggaatgca aaagaatgca aaggaataat ggagccggtg
    12001 ggagtcgaac ccacgtccaa acacctgcca acatatttgt ctacaaccat aggttatgta
    12061 ttattttaac aacccctcga cacataactc aagtctagaa actgcgagtc tatcaatctc
    12121 ttatcaagcc gctagacaag gcttaatcgt atctcgctaa taataagacc tgtcattgaa
    12181 cacgagcaat ccaaatcggg tcacgcctgc tggtttttag gcagctagag cgtaagaagt
    12241 gttatttttt gcagttatat ttaactgagc gtttacgtcg ccacacgagt cgcaaaatat
    12301 gcctcataat gcctgtcgaa tccgtaacga ccccaaaaga caataaaacc attatacctt
    12361 atctaactca aaaatgcaaa aagaaatcac atacctaaaa aagatactat tcgagaaatt
    12421 tagcaaactg gaagactaac tacaactttc tcagtgcata actttacgat tgtaaataga
    12481 gcttacgaaa tccaattaac acatacctaa gatgaagcta tctgttttga agaaattcac
    12541 gaagagaatt attctaaaat actttcagat gagaattttc gtactctctt tattttaatt
    12601 catttgagtt taaaaactat aaaatcaatc cttatattat tcggactact tttcacttat
    12661 tgcaccaaaa tatagtaaaa
//
LOCUS       DAVLFB010000053        12584 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLFB010000053 DAVLFB010000000
VERSION     DAVLFB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.053"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            14..406
                     /locus_tag="VXD58_001334"
     CDS             14..406
                     /locus_tag="VXD58_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676018.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            428..1219
                     /locus_tag="VXD58_001335"
     CDS             428..1219
                     /locus_tag="VXD58_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676019.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1212..1853
                     /locus_tag="VXD58_001336"
     CDS             1212..1853
                     /locus_tag="VXD58_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676020.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            1857..2489
                     /locus_tag="VXD58_001337"
     CDS             1857..2489
                     /locus_tag="VXD58_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676021.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2517..2804
                     /locus_tag="VXD58_001338"
     CDS             2517..2804
                     /locus_tag="VXD58_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676022.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            2950..5463
                     /gene="secA"
                     /locus_tag="VXD58_001339"
     CDS             2950..5463
                     /gene="secA"
                     /locus_tag="VXD58_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV0676023.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5544..6575
                     /locus_tag="VXD58_001340"
     CDS             5544..6575
                     /locus_tag="VXD58_001340"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0676024.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6577..7608
                     /locus_tag="VXD58_001341"
     CDS             6577..7608
                     /locus_tag="VXD58_001341"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV0676025.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7647..8015
                     /locus_tag="VXD58_001342"
     CDS             7647..8015
                     /locus_tag="VXD58_001342"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV0676026.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8005..9108
                     /locus_tag="VXD58_001343"
     CDS             8005..9108
                     /locus_tag="VXD58_001343"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV0676027.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9127..11142
                     /gene="recG"
                     /locus_tag="VXD58_001344"
     CDS             9127..11142
                     /gene="recG"
                     /locus_tag="VXD58_001344"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV0676028.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLI
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLALIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11459..12439
                     /locus_tag="VXD58_001345"
     CDS             11459..12439
                     /locus_tag="VXD58_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV0676029.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3664 a         2356 c         2877 g         3687 t
ORIGIN      
        1 ggaaacagaa aaaatgaaaa ttaagaaatt attgaaaatg gttattcctg ttttaatgat
       61 aagtgctgtt ggaactactt tcgtagaagc aaatcagata ggtgctttta gtaattttgt
      121 tattactacc agttataaga gaacaggtta tttgactaaa gaaaatgaag gtgcggaata
      181 tatcatgaac ttaaatcctt gtagaaattt acatcccatg actgttaagc atcgtatagt
      241 aaattctaat ggagaagctc gtagtggaga atcgttaaca acctgtggta ctcgttcaac
      301 tcatggtaat tgggcaacgg ttggctatgt ttatgcagca gatatggcaa gacaaaattg
      361 gtgggattta tcagctgcca tttcaggtag ttggtcacca aattaattcg taaagtaggt
      421 ataatttatg aagattcctc tcttaacttt ggcaaggcat aaatttgttt atgtcttgct
      481 tactttgctt tttcttgctt tggtttatcg tgacgttttg atgacttatt tcttttttga
      541 tattcatgcg cccgatctag ctaaattcga tggacaagca attaaaaatg acttattaaa
      601 atcagcatta gattttcgta ttctccagtt caatctaggt ttttatcaat catttattat
      661 tccaatcatc attgttttgc taggttttca atatattgag ctgaaaaata aagttttacg
      721 attgagtatt ggaagagaag tgagctatcc agggttaaaa agaaagttga ctttgcaagt
      781 tgcaagtatc ccttgtttga tatatttagt gactgtgctg acaattgcaa ttataaccta
      841 tttccttggg actttttctc ctcttggatg gaattctcta ttttctgatg gaagtggttt
      901 acaaagactc ctagatggag agataaaaag ctatttgttc tttacttgtg tcctgctaat
      961 cggtattttc atcaatgcaa tctatttttt acaaatagtt gattatgtgg ggaatgtgac
     1021 tcgttcggca atcacctatt tgatgtttct ttggcttggt tctatgctgc tctatagtgc
     1081 cttgccttac tatatggttc ctatgacgag tttgatgcaa gctagctatg gggatgtaag
     1141 tttgatgaaa ctctttactc cttatatcct ttatattgtc ccttacatgg tgcttgaaaa
     1201 atatgaagat aatgtttaag aattttaaca atattttgct aaagagaaag attgttttac
     1261 tacttcgtat agttctgatg atgattttga taaaccatct attgtcaaca gcggttcaaa
     1321 agcaggatgc tgttatcttt ttcaagagag aattgatttc aattttttcc tataatgact
     1381 attctgaagc gaatttagaa atccccaaac tcttgttaaa cctttcgctt ttcatggtag
     1441 gatggctctc tgtcatttta cttgaaagtg atttggcaga ccattaccat cacttgattc
     1501 gctatcaatc aagctccttt ttcgattata caaggaaacg attggttgtc atttctaaat
     1561 tttttactca agatttgttt gtctggttcc ttggtttact tccgctagga attcatttca
     1621 aaacagtcgc acttttcttt ttacttgctc agttaatgat gttgtactta ctactgtctt
     1681 atctgatagc actgattagt gcgggcgctg gtttttcctt ttttctctat tttttagtat
     1741 ttgtgggaca agaatggatg atggatcata ttgtaacagt gtatttacta ctcttaagtt
     1801 tattagttat gttgattgtt agtcgcttgg aagagaaatt taagaaagga taaacgatga
     1861 gacttgaaat tataaatgga cagaaaattt atgggaaaag acctatttta aatcagttga
     1921 atttggtgtt tcaatcagga aaaatttatg gacttaaagg tgataatgga tctggcaaga
     1981 cggttctttt aaaggtactt gctggctata ttaagcttga caaaggaaaa gttcttcaag
     2041 acgggaaagt ctacgggata aaaaatcatt atattcagga tgcaggaatt ttaattgaaa
     2101 aagtcgagtt tttatctcat ttatccctga gagaaaattt ggaactgtta aggtattttt
     2161 catctaaagt tacggaaaaa agaattgcct attggattca atactatgat ttacaggaat
     2221 ttgaagacgt tgaataccgt catttatcct tgggaacaaa gcaaaaaatg gccttgattc
     2281 aagcctttat ttcctctcct tctatactct ttctcgatga acctatgaat gctttggatg
     2341 agaagagtgt gaggttaacc aaacaggtca ttttatctta cctgaaaaaa gaaaatggtc
     2401 tggttatcct gacgtcgcac atatcggaag atatttcaga cctttgtaca gatgtattag
     2461 ttgtcgaaaa tggacatata caaatgtaaa ggatatacaa tcctaggaga tggcttatgg
     2521 cacatctaaa atcatttatt acacgctatt ccaaggttta tattggttta gttctgctga
     2581 tctggctgtc tttcttcttt atcccttggg atagagcgat tctgggggta aggattgaca
     2641 tcttcatcat acagaaaatc ttgctagctt ttggaattct gtccattctc atggccttgc
     2701 tgtccaagaa agtcagtctc tttgtttttg gactgatttg ctgtctttct ctttggatta
     2761 acttatttat cacatttgcc attttgccga tttttggcaa ttaaacagtc ataaaagtcg
     2821 gagaggttag cttgaaaact aacctctttt tccttttcaa aatggggatt cttccttgaa
     2881 aataatcagt aattgtgcta aaattaaagg aacattctaa aatattcgga atttaaagta
     2941 aggaaaaaca tggctaatat tttaaaaaca attatcgaaa atgataaagg agaaatccgt
     3001 cgtctggaaa agatggctga caaggttttc aaatacgaag accaaatggc tgctttgact
     3061 gacgaccaac taaaagcaaa aacagttgaa tttaaggaac gttatcaaaa tggagaatca
     3121 ctggattcat tgctttacga agcatttgcg gttgtccgtg aaggtgccaa acgtgtccta
     3181 ggtctcttcc cttataaggt tcaggtcatg ggggggattg ttcttcacca tggtgacgtg
     3241 ccagagatgc gtacagggga agggaaaacc ttaactgcga ccatgccggt atacctcaat
     3301 gccctttcag gtaaaggggt tcacgtagtt acggttaatg aatacctgtc agaacgtgac
     3361 gcgactgaga tgggtgaatt gtactcttgg cttggtttgt cagtagggat taacttggct
     3421 accaaatctc caatggagaa aaaagaagcc tatgagtgtg atattactta ctcaactaac
     3481 tcagaaatcg gatttgacta ccttcgtgac aacatggtcg ttcgcgctga aaacatggta
     3541 caacgtccgc ttaactatgc cttggtcgat gaggttgact ctatcttgat tgacgaggct
     3601 cgtacacctt tgattgtatc aggtgccaat gcggttgaaa ccagtcagtt gtatcacatg
     3661 gcagaccact atgtaaaatc tttgaacaaa gatgactaca tcatcgatgt gcagtctaag
     3721 actattggtt tgtctgattc agggattgac agggctgaaa gctacttcaa acttgaaaac
     3781 ctctatgaca tcgaaaacgt ggctttgacc cactttatcg ataacgccct tcgtgccaac
     3841 tacatcatgc ttctcgatat tgactatgtg gtgagcgaag agcaagaaat cttgattgtc
     3901 gaccaattta caggtcgtac catggaaggt cgtcgttatt ctgatggatt gcaccaagct
     3961 attgaagcca aagaaggtgt gccaatccag gatgaaacca agacatctgc ctcaatcacg
     4021 taccaaaacc ttttccgtat gtacaaaaaa ttgtctggta tgacgggtac aggtaagact
     4081 gaggaagaag aatttcgtga aatctacaac attcgtgtta ttccaatccc aacaaaccgt
     4141 cctgttcaac gtattgacca ctcagacctt ctttatgcaa gtatcgaatc taagtttaaa
     4201 gcggttgtcg aagacgttaa ggctcgttac caaaagggtc aacctgtctt ggttggtaca
     4261 gtagcggttg aaactagtga ctacatttct aagaaattgg ttgcagctgg tgttcctcac
     4321 gaagtcttga atgccaaaaa ccactataga gaagcccaaa tcatcatgaa tgctggtcaa
     4381 cgtggtgccg ttaccatcgc aaccaacatg gcgggtcgtg gtaccgacat caagcttggt
     4441 gaaggtgttc gtgaacttgg aggactttgt gttattggta cagaacgtca tgaaagtcgt
     4501 cgtatcgata accagcttcg tggacgttca ggtcgtcaag gagatccagg tgagtcacaa
     4561 ttctacctat ctcttgaaga tgatttgatg aaacgttttg gttctgaacg cttgaaggga
     4621 atctttgaac gtctcaacat gtctgaagag gccattgagt ctcgcatgtt gacgcgtcag
     4681 gttgaagcag ctcagaaacg tgtcgaagga aataactacg atacccgtaa acaagtcctt
     4741 caatacgatg atgtcatgcg tgaacaacgt gagattatct atgctcaacg ttacgatgtc
     4801 atcactgcag atcgtgactt ggcacctgaa attcagtcta tgatcaaacg cacgattgaa
     4861 cgtgtcgttg atggtcatgc gcgtgccaaa caagatgaaa aactagaggc aattttgaac
     4921 tttgctaagt acaacttgct tcctgaagat tctattacga tggaagactt gtcaggcttg
     4981 tctgataagg ccatcaagga agagcttttc caacgttcct tgaaggttta cgatagtcag
     5041 gtttcaaaac tacgcgatga agaagcagtt aaagaattcc aaaaagtttt gattctacga
     5101 gtggtggata acaagtggac agatcatatc gatgcccttg atcaattgcg taacgcggtt
     5161 ggacttcgtg gctatgctca gaacaaccct gttgttgagt atcaggcaga aggtttccgt
     5221 atgtttaatg atatgattgg ttcgattgag tttgatgtga cacgcttgat gatgaaagca
     5281 caaattcatg aacaagaaag accacaggca gaacgtcata tcagtacaac agcgactcgc
     5341 aatatcgctg ctcaccaagc aagtatgcca gaagatttgg atttgagcca gattggacgc
     5401 aatgaacttt gcccatgtgg ttctggtaag aaatttaaaa actgtcacgg taaaagacaa
     5461 taaaatgaga tagtttagag gcggatatct tgtgaaaagt aaatttttac tgggtatccg
     5521 tttgctttat aaggagatga gttatggtat ttacagcaaa aagctctaaa ataaatatag
     5581 aagaagttcg tgccttgtca aaattagaag gtcaggcttt ggagaggaaa tcacagcgag
     5641 atcaagagct agaagccatt atacgtggag aagaccagcg aattctcttg gtaatcgggc
     5701 catgctcatc tgacaacgaa gaagctgtcc ttgaatacgc taagcgtttg gcagtcctac
     5761 aagaagaagt ggcagatcgt atctttatgg ttatgcgtgt ttatactgcc aaaccccgta
     5821 ccaacggaga tggctataag ggcttgattc accagcctaa cgcgacagaa gcgcctagtc
     5881 ttatcaatgg aatcaaagcc gttcgccatc ttcactatcg tgtcatcaca gaaacaggga
     5941 tgacaactgc tgatgaaatg ctttatcctg aaaaccttcc gcttgtagat gatttgattt
     6001 cttacatggc agttggtgcc cgttcagttg aagaccagca acaccgcttt gtggcaagtg
     6061 gggcaggatt ttctactggt tttaaaaatc caacctctgg aaatctcaat gtcatgttta
     6121 atgggattta tgctgctcaa aacaaacaaa gtttcctttt cttaggaaaa gaagtagaaa
     6181 caactgggaa cccgctttca cacgctattc ttcgtggtgc tcttaatgag tatggaaaaa
     6241 atattcccaa ctactattat gacaatttaa ttgataccat tgcccagtat gagaaaatgg
     6301 gcttggaaaa tccttttatc atcattgata ccaatcatga caattctggt aagcagtata
     6361 ttgaacagat ccgaattgtc cgccagacct tgattaaccg tgcttggaat gaaaaaatta
     6421 agcagttcgt tcgtggtttt atgattgagt cttatctgga agatggtcga caaaatgagc
     6481 cagaagtatt tggtaagtct atcacagacc cttgcctggg ttgggataac acagaagctc
     6541 ttgtcagaga aatttacaaa acgttaggag aataagatgg catttattga aaaaggtcaa
     6601 gaaatcgata tggaagtcat caaggctgaa acccaattgt ctgcggaagc cttgagactc
     6661 aaggaaagcc gtgacaggga attggcagat attatttcag gggaagatga ccgtattctc
     6721 ttggtgattg gtccttgctc ttctgataat gaagaggcgg tcttggaata tgctcgccgt
     6781 ttatctgcct tgcaaaagaa ggtagcggat aagattttca tggtcatgcg cgtgtatact
     6841 gctaagcctc gtaccaatgg agacggctat aaaggattag ttcaccagcc agatacttct
     6901 aaggctccaa gcctgattaa tggcttgcag gctgtgcgcc agttgcacta ccgcgtgatt
     6961 acagagactg gtttgacaac ggcagatgag atgctttatc cgtcaaatct gatcttggtg
     7021 gatgacttgg tcagctacca tgccgttgga gctcgttctg tggaagacca agagcaccgc
     7081 tttgtggctt ctgggattga tgcaccagta gggatgaaaa atccaacctc aggaaatttg
     7141 ggtgttatgt ttaacgccat ctatgctgct caaaacaagc aaaccttcct ttatcatggg
     7201 caggaagttg agacatcagg taatcctttg gcccatgtta tcctccgtgg agcagtcaac
     7261 gagtatggca attatatgcc gaattactac tatgaaaatc tactccaagc cattgaacgc
     7321 tatgaaacca tgggacttga aaatcctttt atcctcattg acaccaacca tgataactca
     7381 ggcaagcaat atatggagca gattcgaatt gttcgccaga ccttgcagaa tcgtgattgg
     7441 aatgagaaaa ttaaaaagac ggttcgagga tttatgattg aatcttacct agcagatggt
     7501 cgtcaaaacc aaccagagat ctttggttgc tctattactg acccttgcct aggttgggaa
     7561 aatacagagg ccttggtaga agagatttat gttaccttga caaaataagt gaaaaggatg
     7621 gagttgggga atctcaactc cttttgatga gaatgatagt tggacacgga attgacatcg
     7681 aagaattggc ttcgatagaa agcgcagtta cacgacatga aggatttgct aagcgtgtac
     7741 tgaccgctca ggaaatggag cgcttcacca gtctcaaagg acgcaggcaa atagaatatt
     7801 tagctggtcg ctggtcggct aaggaggcct tttccaaggc tatgggaacg ggcattagca
     7861 agctcggttt tcaggatttg gaagtcttga acaatgaacg tggggcgcct tattttagtc
     7921 aggcaccatt ttcaggaaag atttggctgt ctatcagcca caccgatcag tttgtgacag
     7981 ccagtgtcat tttggaggaa aatcatgaaa gctagtccac atagaccaac caaggctctg
     8041 attcatctgg gagctattcg acaaaatatt cagcaaatgg gggctcatat ccctcaagga
     8101 acgctcaagt tggctgtggt taaggccaat gcttatggtc atggagctgt tgccgttgcc
     8161 aaggcaattc aagatgatgt tgatggcttt tgcgtttcca atatcgatga agccattgaa
     8221 ctcagacaag ctggactcag caagccaatc ctcattttag gagtttctga aatcgaagct
     8281 gttgctctag ctaaagaata tgacttcacc ttgacagtgg ctggactgga gtggattcaa
     8341 gcactcttag ataaggaagt ggacctaact ggattgacag tccacctcaa gattgattca
     8401 gggatgggac ggattggttt tagagaggca agtgaggttg agcaggctca agatttgctc
     8461 caacaacacg gtgtttgtgt tgaaggaatc tttacccact ttgctactgc tgatgaggaa
     8521 tcagatgact attttaatgc ccagttagaa cggtttaaaa ctattttagc tagtatgaag
     8581 gaagttccag agctggttca tgctagcaat tctgcaacga ctctttggca tgtagagact
     8641 attttcaatg cggttcgtat gggagatgcc atgtatggcc tcaatccaag tggagcggtc
     8701 ttggatttgc cttatgattt gataccggcc ttgaccttgg agtctgctct ggttcatgtc
     8761 aagacagttc cagctggagc ttgcatgggc tatggagcaa cttatcaagc ggatagcgag
     8821 caagtcatcg cgaccgtgcc aatcgggtat gcagatggat ggacaagaga catgcaaaat
     8881 ttctctgtct tggtagatgg ccaagcttgc ccaattgtcg gcagggtttc gatggaccaa
     8941 atcactattc gattgcctaa gctttatccg ctaggaacca aggtaacctt gattggctcc
     9001 aatggggata aggaaatcac tgcaactcag gtagcgacct accgcgtaac cattaactat
     9061 gaggtggttt gcctcctcag cgaccgtatt ccgagagaat attattagaa aagaaaggag
     9121 tggagcatga atctacatca acccttgcat gtcttgcctg gtgtgggacc aaagtcagca
     9181 gaaaaatacg ccaaactagg aattgaaaac ttgcaagatc tcttgctcta ctttcctttc
     9241 cgttatgaag acttcaaaac caagcaggtg ctggagctgg aagacggtga gaaggcagtt
     9301 ctttctggtc aggtagtgac tcctgctagt gtccagtatt atggtttcaa gcgcaatcgc
     9361 ctgcgtttta gtctcaagca gggagaggtc gtttttgcgg tgaatttctt taaccagccc
     9421 tatctggctg ataaaataga gttgggagca acccttgctg tctttggaaa atgggaccgc
     9481 gctaaggcta gtctgactgg gatgaaggtt ctggctcagg tagaagatga cctccagcct
     9541 gtctatcgtc tggctcaggg aatcagtcag gccagtctgg tcaaggtcat caagacggct
     9601 tttgatcagg gactggacct cttgatagaa gaaaatctgc cccagtcttt actagacaaa
     9661 tacaaactca tgtcccgttg tcaggcagtc cgagctatgc attttccaaa ggatttggca
     9721 gaatacaagc aggctcttcg ccgtataaag tttgaggaac tcttttattt ccaaatgcag
     9781 ctgcagatgc tcaagtctga aaatagagtt cagggaagtg gtctggttct gaattggtct
     9841 caggaaaaag tgacagcagt taaagtaagt cttccttttg ccctgaccca agctcaggaa
     9901 aagagtttgc aggaaatttt aactgatatg aagtccgacc accacatgaa tcgtctccta
     9961 caaggggatg tggggagtgg aaaaacggta gtcgctggct tggccatgtt tgcggcagtg
    10021 acagcaggtt atcaggctgc cctaatggta ccaacagaaa tcctcgcaga gcaacacttt
    10081 gagagtttac agaacctttt tcccaatttg aaactggctc tcttgatagg ttccttgaaa
    10141 gctgcagaaa agagagaggt cttggagacc attgccaagg gtgaggctga tttgattatc
    10201 ggaactcacg ctctgataca agatggggtg gagtatgctc gtcttgcttt gattattatc
    10261 gatgagcagc accgttttgg tgtagggcaa aggcgtattt tacgggaaaa aggtgacaat
    10321 ccagatgtcc tcatgatgac ggcgactccc attccacgga cgcttgccat cacagccttt
    10381 ggagatatgg atgtttccat tatcgaccag atgccagcag gtcggaagcc tattgtgacg
    10441 cgctggatca aacatgagca actacctcag gtcttgactt ggttagaggg ggaaattcaa
    10501 aaaggttccc aagcctatgt catctctcct ttgattgaag aatcagaagc tctagatttg
    10561 aaaaatgcca ttgccttatc agaggagttg acgactcatt ttgcaggcaa ggcagaggtg
    10621 gctcttctac atggtaggat gaagagtgac gaaaaagacc agatcatgca ggatttcaag
    10681 gagagaaaga cggatattct ggtttcgacg acggttattg aggttggggt caacgttccc
    10741 aatgcgactg tcatgattat catggatgcc gatcgcttcg gtctcagtca acttcaccag
    10801 cttagaggtc gtgtcggtcg gggggacaag cagtcctacg ctgttctcgt tgctaatccc
    10861 aagacggatt ctgggaaaga ccgcatgcgt atcatgacag aaacgaccaa tggatttgtc
    10921 cttgcggagg aagatttgaa aatgcgtggt tctggtgaga tttttggaac cagacagtca
    10981 ggacttccag agttccaagt ggctgatatt atcgaagatt ttccgatttt agaagaagca
    11041 agaaaggttg ctagctacat tagttctata gaagcttggc aagaagatcc agagtggcgc
    11101 atgattgccc ttcatctgga aaagaaagaa catctggatt aagctttctc taaggaaaac
    11161 ttaagctagt ttttaggttt ggctcttata ctaaagtcat caaaaagaaa cgaggactct
    11221 catatgacag taacgattaa agtaaattac caaaccactt tccaaaagaa ggaagcaaaa
    11281 aactagtata aacagaagag agagcgaaat gctctttttt cgtttctaaa actactttca
    11341 gcccatcatc ctaaaagtaa agaatctaaa ttcactttct atttaccctt ctttcttgca
    11401 ttgattacat agatatgcta cagttgtggt aacgattaca aaataaaagg agcatgctat
    11461 gaaaaatcca gctttgctag aagaaattaa gacctataga ggaagggatg aggttccgga
    11521 agactttgat gctttctggg atggggaagt gaaaaatgtt tccacacttc catcctacca
    11581 cttggaggaa agagatttcc acattcctca agtcaagtgc tatgagttaa catttgaagg
    11641 aagcaaggaa ggaaaggtct atgcacgcat tgttcttcca aagagtgagg agaaggttcc
    11701 attaatcttc cattttcatg gttatatggg acgtggctgg gactgggccg acatgctggg
    11761 cttcaccgta gctggttacg gtgttgtttc catggatgtg cggggccagt caggttactc
    11821 acaagacggc ttgcgttctc ctttaggaaa taccgtgaag gggcatatta tccgtggtgc
    11881 tatggaaggt cgggaccacc tcttttataa ggatgtttat ctggatattt accagttggt
    11941 cgaaattgtt gctagtctgt ctcaggttga tgagaagcgt ctttctagct atggtgcctc
    12001 acaaggaggg gctctagctc tagttgcagc agcgctcaat cctcgaattc agaaaacagt
    12061 tgccatttat cccttcttgt cagacttcag acgggtgatt gagattggta atactagcga
    12121 ggcttacgac gaacttttcc gttatttcaa gtttcacgac cccttccatg aaacagagga
    12181 ggaaatcatg gcgacccttg cctatatcga tgtcaaaaat cttgcccatc gtatccaagg
    12241 tgaggttaag atgattacgg gcttggacga cgatgtttgc tatcccatta cccagtttgc
    12301 gatttataat cgtctgacct gcgataaaac ctatcgcatc atgcctgagt atgctcacga
    12361 agccatgaat gtatttgtca atgaccaagt ctacaactgg ctctgtggaa gtgagattcc
    12421 ttttaaatat ctaaaataag gagtcgactc taagcacaaa atcttaaaaa ttacaaacac
    12481 gcatagtatc aggggattaa aaaaacttga tactatgcgt tttatcatgg aaatatagta
    12541 aaatgaaaca agaataggac aaaacgatca ggacagtcaa atcg
//
LOCUS       DAVLFB010000054        12532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLFB010000054 DAVLFB010000000
VERSION     DAVLFB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.054"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..267
                     /locus_tag="VXD58_001346"
     CDS             1..267
                     /locus_tag="VXD58_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGC"
                     /protein_id="HEV0676030.1"
                     /translation="MLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAEL
                     YSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGANRKVND"
     gene            260..664
                     /locus_tag="VXD58_001347"
     CDS             260..664
                     /locus_tag="VXD58_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV0676031.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            669..929
                     /locus_tag="VXD58_001348"
     CDS             669..929
                     /locus_tag="VXD58_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676032.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            892..1353
                     /locus_tag="VXD58_001349"
     CDS             892..1353
                     /locus_tag="VXD58_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0676033.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            1331..1777
                     /locus_tag="VXD58_001350"
     CDS             1331..1777
                     /locus_tag="VXD58_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV0676034.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            1880..2467
                     /locus_tag="VXD58_001351"
     CDS             1880..2467
                     /locus_tag="VXD58_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0676035.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            2528..3481
                     /locus_tag="VXD58_001352"
     CDS             2528..3481
                     /locus_tag="VXD58_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0676036.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            3532..4722
                     /locus_tag="VXD58_001353"
     CDS             3532..4722
                     /locus_tag="VXD58_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV0676037.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYFAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            4871..5242
                     /gene="rnpA"
                     /locus_tag="VXD58_001354"
     CDS             4871..5242
                     /gene="rnpA"
                     /locus_tag="VXD58_001354"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV0676038.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            5217..6041
                     /locus_tag="VXD58_001355"
     CDS             5217..6041
                     /locus_tag="VXD58_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV0676039.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            6060..7046
                     /locus_tag="VXD58_001356"
     CDS             6060..7046
                     /locus_tag="VXD58_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV0676040.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            7097..7720
                     /locus_tag="VXD58_001357"
     CDS             7097..7720
                     /locus_tag="VXD58_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV0676041.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            7851..9308
                     /locus_tag="VXD58_001358"
     CDS             7851..9308
                     /locus_tag="VXD58_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV0676042.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            9331..9612
                     /locus_tag="VXD58_001359"
     CDS             9331..9612
                     /locus_tag="VXD58_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0676043.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            9689..10174
                     /locus_tag="VXD58_001360"
     CDS             9689..10174
                     /locus_tag="VXD58_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV0676044.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            10191..10856
                     /locus_tag="VXD58_001361"
     CDS             10191..10856
                     /locus_tag="VXD58_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV0676045.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            10860..11723
                     /locus_tag="VXD58_001362"
     CDS             10860..11723
                     /locus_tag="VXD58_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV0676046.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            11725..12429
                     /locus_tag="VXD58_001363"
     CDS             11725..12429
                     /locus_tag="VXD58_001363"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV0676047.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         3893 a         2218 c         2834 g         3587 t
ORIGIN      
        1 atgttagtag tcttgctgat tatcagcgtg cttttcttgc tctttgtacc taatctgacc
       61 aagcaaaaag aagcagtcaa tgacaaagga aaagcagctg ttgttaaggt ggtggaaagc
      121 caggcagaac tttatagctt ggataaaaat gaagatgcta ctctgagcaa gttacaagga
      181 gatgggcgca tcacggaaga acaggttaaa gcttataatg aatactatac taaaaatgga
      241 ggggcaaatc gtaaagtcaa tgattaaggc ctttaccatg ctggaaagtc tcttggtttt
      301 gggacttgtg agtatccttg ccttgggctt atccagctct gtccagtcca cttttgcagc
      361 agtagaggag cagattttct ttatggagtt tgaagaactc tatcgggaaa cccaaaaacg
      421 cagtgtagcc agtcagcaaa agactagtct aaatttagat gggcagacgc ttagcaatgg
      481 cagtcaaaag ttgacagttc ctaaaggaat tcaggcacca tcaggccaaa gtattacatt
      541 tgaccgagct gggggcaatt cgtccctggc taaggttgaa tttcagacca gtaaaggagc
      601 gattcgctat caattatatc taggaaatgg aaaaattaaa cgcattaagg aaacaaaaaa
      661 ttagggcagt gattttactg gaagcagtag tcgctctagc tatctttgcc agcattgcga
      721 ccctcctttt gggacaaatt caaaaaaata ggcaagagga agcaaaaatc ttgcaaaagg
      781 aagaagtctt gagggtagct aagatggccc tgcagacggg gcaaaatcag gtaagcatca
      841 acggagttga gattcaggta ttttctagtg aaaaaggatt ggaggtctac catggttcag
      901 aacagttgtt ggcaatcaaa gagccataag gtcaaggctt ttaccttgtt agaatccctg
      961 cttgccctca ttgtcatcag tgggggatta ctcctttttc aaactatgag tcagctcctc
     1021 atttcagaag ttcgctacca gcaacaaagc gagcaaaagg agtggctctt gtttgtggac
     1081 caacttgagg cagaattaga ccgttcgcag ttcgaaaaag tagaaggcaa tcgcctatac
     1141 atgaagcaag atggcaagga catcgccatc ggtaagtcaa agtcagacga ttttcgtaaa
     1201 accgatgcta gtggacgggg gtatcagcct atggtttatg gcctcaaatc agctcagatt
     1261 acagaggaca atcaactggt tcattttcgt ttccagtttc aaaaaggctt agaaagggag
     1321 ttcatctatc gtgtggaaaa agaagaaagt taaggcaggt gttctcctct acgcagtcac
     1381 catagcagcc atctttagtc ttttgttgca attttatttg aaccgacaaa tcgcccacta
     1441 tcaagactat gctttaaata aagaaaaatt ggttgctttt gccatggcta aacgaaccaa
     1501 ggataaagcc gagcaagaaa gtggggaaca ggtttttaat ctaggtcagg taagctatca
     1561 aaacaagaaa acaagcttgg tgacgactgt tcgtacgtct aagagccaat atgagtttct
     1621 gtttccttca gtcaaaatca aagaagagaa aagagataaa aaggaagagg tagcgaccga
     1681 ttcaagcgaa aaagcggaga aaaaaaatca gaagagaagc ctgaaaagaa agagaattcc
     1741 tagtcaattc aactataatg cgttgaatcc agaatagtcc actgtagttt ctagaaaatt
     1801 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
     1861 tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgactc
     1921 ccctaaaaat atcttcctcg caaacttggt atgtcaagca gtcgagaaac agattgatat
     1981 tttatcagac aaagtaattt tagatttcgg tggtggcacg ggtctattag ccttgcccct
     2041 agccaagcag gctaagtcag ttactcttgt agacatttct gagaaaatgt tggagcaagc
     2101 tcgtttgaaa gtggagcagc aagcaatcaa gaatatccag tttttggagc aagatttacc
     2161 gaaaaatccc ttggagaaag agtttgattg ccttgctgtt agtcgggttc ttcatcatat
     2221 gcctgatttg gatgcggctc tctcactgtt tcatcaacat ttgaaggaag atgggaaact
     2281 catcattgct gattttacca agacagaagc taatcatcat ggatttgatt tagctgaact
     2341 ggaaaacaag ctaattgagc atggtttttc atctgtgcat agtcagattc tctatagcgc
     2401 tgaagacctg tttcaaggaa atcactcaga attcttttta atagtagccc aaaaatcact
     2461 cgcctagtca gggagtgatt tttctataag gatggaaaaa agaagggaaa tttgataaga
     2521 taggaatatg gattttgaaa aaattgaaca agcttatacc tatttactag agaatgtcca
     2581 agttatccaa agtgatttgg cgaccaactt ttatgacgcc ttggtggagc aaaacagcat
     2641 ctatctggat ggtgaaactg agctaaacca ggtcaaagac aacaatcagg cccttaagcg
     2701 tttagcacta cgcaaagaag aatggctcaa gacctaccag tttctcttga tgaaggctgg
     2761 gcaaacagaa cccttgcagg ccaatcacca gtttacacca gatgccattg ctttgctttt
     2821 ggtgtttatt gtggaagagt tgtttaaaga ggaggaaatt actatcctcg aaatgggttc
     2881 tgggatggga attctaggcg ctactttctt gacctcgctt gataaaaagg tggattactt
     2941 gggaatggaa atggatgatt tgctgattga tctggcagct agcatggcag atgtaattgg
     3001 tttgcaggct ggctttgtcc aaggagatgc cgttcgtcca caaatgctca aagaaagcga
     3061 tgtggtcatc agtgacttgc ctgttggcta ttatcctgat gatgccgttg cgtcgcgcca
     3121 tcaagttgct tctagccaag aacatactta cgcccatcac ttgctcatgg aacaagggct
     3181 taagtacctc aagtcagacg gatacgctat ttttctagct ccgagtaatt tgttgaccag
     3241 tcctcaaagt gatttgttaa aagaatggct gaaagaagag gcgagtctgg ttgctatgat
     3301 tagtctgcct gaaaatctct ttgctaatgc caaacaatct aagactattt ttatcttaca
     3361 gaagaaaaat gaaatagcag tagagccttt tgtttatcca cttgctagct tgcaagatgc
     3421 aagtgtttta atgaaattta aagaaaattt tcaaaaatgg actcaaggta ctgaaatata
     3481 aaatagattt tgttataata gttgaaaacg cttaaaaagg ggtatcatgt tatgacaaaa
     3541 acaattgcaa tcaatgcagg aagttcaagt ttgaaatggc aattatactt aatgccagaa
     3601 gaaaaagtgt tagccaaagg tttgattgaa cgtatcggtt tgaaagattc aatttcaact
     3661 gtaaaatttg acggccgttc tgaacaacaa attttggata ttgaaaatca tacacaagcc
     3721 gttaaaattt tattggatga cttgattcgt ttcgatatta tcaaggctta tgacgagatt
     3781 acaggtgttg gacatcgtgt tgttgctggt ggagaatatt tcaaagaatc aacagttgtt
     3841 gagggagatg ttttagaaaa agttgaagag ttgagtttgt tggctcctct acacaacccg
     3901 gccaatgcag caggtgttcg tgccttcaag gaattgttgc cagacattac cagtgtagtt
     3961 gtttttgata cttccttcca cacaagtatg ccagagaaag cttatcgcta ccctctacca
     4021 acaaaatatt acacagaaaa caaggttcgt aaatacggtg ctcatggtac aagtcaccag
     4081 tttgtagcag gagaagctgc aaaactcttg ggacgtccat tagaagactt gaagttaatt
     4141 acctgtcata ttggtaacgg aggctcaatt acagctgtga aagccggcaa atctgtagac
     4201 acttctatgg ggttcactcc tcttggtggt attatgatgg gaacgcgtac aggggatatt
     4261 gatccagcta tcattcctta tttaatgcaa tatacagagg attttaacac accagaagat
     4321 atcagtcgtg ttcttaaccg tgaatcaggt cttttgggag tttctgctaa ttctagcgat
     4381 atgcgcgata tagaagcagc tgtagcagaa gggaatcacg aggctagctt ggcttatgaa
     4441 atgtatgttg accgtatcca aaaacatatc ggtcagtact ttgcagtgct aaatggagca
     4501 gatgccattg ttttcacagc aggtgtcggt gaaaatgcag agaatttccg tcgtgatgta
     4561 atctcaggga tttcgtggtt tggttgtgat gttgatgatg aaaagaatgt ctttggcgtt
     4621 acaggagaca tctcaacaga ggcagctaaa atccgtgtct tggttattcc aacagatgaa
     4681 gaattagtca ttgcccgtga cgttgaacgc ttgaaaaaat aagtgaaact aaaaaaatat
     4741 tcaatacaag gagttgggaa agttattttt ccagcttctt tttctgatga aattgtccaa
     4801 aaccttgcta tgattggctt ttttgaaaaa tatggtataa tagtagtaat ttaatagatg
     4861 gagttgagtt ttgaagaaaa actttcgtgt aaaaagagag aaagatttta aggcgatttt
     4921 caaggagggg acaagttttg ctaatcgcaa atttgtggtc taccaattag aaaaccagaa
     4981 aaaccatttt cgagtaggtc tatcagttag caaaaaactg gggaatgccg tcactagaaa
     5041 tcaaattaag cgacggattc ggcatattat ccagaatgca aaagggagtc tggtagaaga
     5101 tgtcgacttt gttgtcattg ctcgaaaagg agtcgaaacc ttgggatacg cagagatgga
     5161 gaaaaatcta ctccatgtat taaaattatc aaagatttac cgggaaggaa atgggagtga
     5221 aaaagaaact aaagttgact agtttgctag gactgtctct gttaatcatg acagcctgtg
     5281 cgactaatgg ggtaactagc gatattacag ccgaatcggc tgatttttgg agtaaattgg
     5341 tttacttctt tgcgaaaatc attcgctttt tatcgtttga tattagtatc ggagtgggga
     5401 ttattctctt tacggtcttg attcgtacag tcctcttgcc agtctttcag gtgcaaatgg
     5461 tggcttctag gaaaatgcag gaagctcagc cacgcattaa ggcgcttcga gaacaatatc
     5521 caggtcgaga tatggaaagc agaaccaaac tagagcagga aatgcgtaaa gtatttaaag
     5581 aaatgggtgt cagacagtca gattctcttt ggccgatttt gattcagatg ccggttattt
     5641 tggccttgtt ccaagcccta tcaagagttg actttttaaa gacaggtcat ttcttatgga
     5701 ttaaccttgg tagtgtggat acaacccttg ttcttccgat tttagcagca gtattcacct
     5761 ttttaagtac ttggttgtcc aacaaagctt tgtctgagcg aaatggcgct acgactgcga
     5821 tgatgtatgg gattccagtc ttgattttta tctttgcagt ttatgcgcca ggtggagtcg
     5881 ccctatactg gacagtgtct aatgcttatc aagtcttgca aacctatttc ttgaataatc
     5941 cattcaagat tatcgcagag cgcgaggccg tagtacaggc acaaaaagat ttggaaaata
     6001 gaaaaagaaa agccaagaaa aaggctcaga aaacgaaata aataaggagg aatctggtaa
     6061 tggtagtatt tacaggttca actgttgaag aagcaatcca gaaaggattg aaagaattag
     6121 atattccaag aatgaaggct catatcaaag tcatttctag ggagaaaaaa ggctttcttg
     6181 gtctatttgg taaaaaacca gcccaagtgg atattgaagc gattagtgaa acgactgttg
     6241 tcaaagcaaa tcaacaggta gtaaaaggcg ttccgaaaaa aatcaatgat ttgaacgagc
     6301 ctgtgaagac ggttagtgaa gaaaccgttg accttggtca tgtggttgat gctattaaaa
     6361 aaatagagga agaaggtcaa ggtatttctg atgaagtcaa ggctgaaatc ttaaaacatg
     6421 aaagacatgc cagcactatc ttagaagaaa ctggtcacat tgagatttta aatgaacttc
     6481 aaatcgagga agcgatgagg gaagaagcag gcgctgatga ccttgaaact gagcaagatc
     6541 aaactgaaaa tcaagacttg aaagagatgg gcttgaaggt cgagcaaagt tatgatattg
     6601 cccaggtggc tacggatgtg actgcctatg ttcaagcgat tgtggatgac atggatgttg
     6661 aagctacact ttcaaatgat tataaccgtc gtagcatcaa tctacaaatt gacaccaacg
     6721 aaccaggtcg tattatcggc taccatggta aagtcttgaa ggccttgcaa ctgttggctc
     6781 aaaattatct ttacaaccgc tattccaaaa ccttctacgt tacaatcaat gtcaatgatt
     6841 atgtcgaaca ccgtgcagaa gtcttgcaga cctatgcgca aaaattggcg attcgtgttt
     6901 tagaagaagg acgtagtcat caaacagatc caatgtcgaa tagtgaacgc aagattatcc
     6961 accgtattat ttcacgtatg gatggcgtga ccagttactc tgaaggtgat gagccaaatc
     7021 gctatgtcgt tgtagataca gaataagtaa aatcagggtt gacctgattt tttgctagct
     7081 agaggaggtt aaactgatgt tgaataagat aagagactat ttagactttg ctggtttgca
     7141 gtaccgtaat cctgataaag cgggagcaga gcgagagaag atgctggcat tccgccacaa
     7201 aggacaagag gcccgaaagg cttttacaga actggccaaa gcctttcaag caagccatcc
     7261 agaatggcaa ctccaacaga ctagtcagtg gatgaatcag gcccagcgtt tgagaccaca
     7321 tttttgggtt tatctacaga gagacggaca agtgacagaa cctatgatgg ccttacgttt
     7381 gtatgggaca tctactgact ttggaatttc tttggaagtc agtttcatcg aacgtaagaa
     7441 ggatgagcaa acactgggca agcaggccaa agttttagac attccaaccg ttaaagggat
     7501 ttattatcta acctactcta atggtcaaag tcaacggtgg gaggcgaatg aagaaaagcg
     7561 tcgtacttta cgcgagaagg tgagaagtca agaagttcga aaagttttag tgaaggtaga
     7621 tgttcctatg acagaaaatt cgtctgaaga agaaatcgta gaaggcttat tgaagtctta
     7681 ttctaaaatt cttccctatt atctagctac gagaaaataa gataatttgt aaaacatcat
     7741 aaatcataca gtccaagagt gaacagtccg ctgtgtaatt cttggtcttt ttgtttgcgc
     7801 tttcgcatta tataataaac ttacaaaaac aattcaaaag gagaacaatt atggaagtcg
     7861 tttcaagtgt tctaaattgg ttttctagca atattttgca gaatcccgca tttttcgtag
     7921 gtttattggt gttgatagga tatgcacttt tgaaaaaacc tgcccatgac gttttttcag
     7981 ggtttgttaa agcaacagta gggtatatgt tgcttaacgt gggtgctggt ggtttggtta
     8041 caacctttcg tccaatctta gcagctctta actacaaatt ccaaattggt gcagcggtta
     8101 tcgaccctta ctttggactt gctgcagcaa acaacaaaat tgcagcagag tttccagatt
     8161 ttgttggaac tgcaactaca gctctattga ttggttttgg aataaatatc ttgctcgtag
     8221 ctcttcgaaa gattacgaag gtaagaaccc tctttattac tggtcacatc atggtacaac
     8281 aagctgcaac agtatctctt atggttctat tcttagtacc acaattgcgc aatgcttacg
     8341 gtacagcagc gattggtatc atctgtggac tttactgggc agttagttca aatatgactg
     8401 ttgaggcaac tcaacgcttg actggtggtg gcggatttgc gattggtcac caacagcaat
     8461 ttgcaatctg gtttgtagat aaagtagcag gacgctttgg taagaaagaa gaaagtttag
     8521 acaatcttaa attacctaag ttcctctcaa tcttccacga tacagttgtt gcatctgcta
     8581 ccttgatgct cgtattcttc ggggccattc ttttaatctt gggtccagac attatgtcta
     8641 ataaagaagt catcacttca ggaactctat tcaatcctgc taaacaagat ttctttatgt
     8701 acattatcca aacagccttt accttctcag tttacttgtt cgttttgatg caaggtgtcc
     8761 gaatgttcgt atctgagttg acaaacgcct tccaaggtat ttcaaacaaa ttgttgccag
     8821 gttcattccc agcggttgac gttgcagctt cttatggatt tggttctcca aatgctgtct
     8881 tgtcaggatt tacctttggt ttgattggtc aattgattac aattgtcttg ctcatcgtct
     8941 ttaaaaatcc gattcttatt attacaggat ttgtaccagt gttctttgac aatgcagcca
     9001 ttgcggtcta cgctgataaa cgcggcggat ggaaagcggc tgttatcctt tcctttatat
     9061 caggtgtcct tcaagttgct ctaggagctc tttgtgtggc ccttctcgat ttggcatctt
     9121 atggtggcta ccatggaaat atcgactttg aattcccatg gcttggattt ggatatatct
     9181 tcaaatacct tggtattgtt ggttatgtac ttgtgtgtct cttcttgctt gttattcctc
     9241 aacttcaatt tgccaaagca aaagataaag agaaatatta caacggtgaa gttcaagaag
     9301 aagcttagta tctagaaaag gagaaataaa atggttaaag tattagcagc gtgcggaaat
     9361 ggaatgggtt catcaatggt tatcaagatg aaggttgaaa atgctctccg taagcttaat
     9421 caaacagatt ttacagtcaa ttcatgcagt gtcggtgaag ctaaaggttt agcagtagga
     9481 tatgacatcg taatcgcttc tcttcatttg attcaagaat tggaagggcg aactaatggg
     9541 aagttaattg gacttgataa cttgatggat gataaagaaa tcaccgaaaa actcagtcaa
     9601 gcactacagt aaaaggttgg agggggctgg acagaaactg agagttatcg tttctgtcct
     9661 tctccctctt taaataaagg aggcagatat gaatttaaaa caagctttaa ttgacaatga
     9721 ctcgatccga ctaggtttag aggctaacaa ttggaaagaa gcagtcaagg tagcagtaga
     9781 tcccttaatt gaaagtgggg caattttgcc agagtattac gatgctatca ttgaatcgac
     9841 tgaagagtat gggccttact atatcttgat gccaggtatg gctatgcccc acgctagacc
     9901 tgaagctggt gtgcaaagtg atgccttttc attgattacc ttacaaaatc ctgttgtatt
     9961 ttcagatggg aaagaggtat ctgttttgtt ggcactagca gcaacaagct caaaaattca
    10021 cacaagtgta gccattccac aaattattgc cctgtttgaa ttagaagatt ctattgcacg
    10081 tttacaggct tgccagacta aagaagatgt cttggctatg attgaagaat ctaaggatag
    10141 cccttatctc gaaggattgg atttggaaag ttagaaagag gaataaagaa atgacaaaaa
    10201 gaatacctaa tttacaagtt gcattagacc attcagactt gcaaggagcg attaaagcag
    10261 ctgtttctgt tggtcaggaa gtagatatta tcgaagctgg aactgtttgc ttgcttcaag
    10321 ttggaagtga actggctgaa gtcttgcgta gccttttccc agataagatt attgtggcag
    10381 acacaaaatg tgctgatgct ggtggaacag ttgctaaaaa taatgcggtt cgtggagcag
    10441 actggatgac ttgtatctgt tgtgcaacca tccctactat ggaagcagct ctaaaggcta
    10501 tcaagactga acgaggagaa cgaggcgaaa tccagatcga gctttatggc gattggactt
    10561 ttgaacaagc tcagctttgg ctagatgcag gtatctcaca agctatttat caccaatctc
    10621 gtgatgctct tcttgctggt gaaacttggg gtgaaaaaga ccttaataag gttaaaaaac
    10681 tcattgacat gggcttccgt gtatctgtaa caggtggtct agatgtagat actctcaaac
    10741 tctttgaagg tgttgatgtc tttaccttta tcgcaggtcg tggaattaca gaggctgcgg
    10801 atccagcagg agcagcgcgt gccttcaagg atgaaatcaa acgaatttgg gggtaaatca
    10861 tggtacgtcc aattggaatt tatgaaaagg caaccccaac acactttact tggctagaac
    10921 gtttaaattt tgccaaggag ttaggctttg attttgtcga gatgtctatt gacgaacgtg
    10981 acgagcgttt agcaagactt gactggagta aggaagaacg cttggaagtt gtcaaagcaa
    11041 tctatgaaac tggtgttcgt attccttcta tctgtttttc aggccatcgt cgctacccat
    11101 tgggttcaaa agatccagtt ctagaggaaa aatctctaga actcatgaaa aaatgtatcg
    11161 aattagctca agacttggga gttcgtacga ttcaattagc tggttacgat gtttactatg
    11221 aggaaaagtc accccagaca cgccaacgtt ttatcaaaaa tttgagaaaa gcctgtgact
    11281 gggctgaaga agctcaggtg gtacttgcta ttgaaattat ggatgatcct ttcatcaata
    11341 gcatcgaaaa atatttggct atagaaaaag agattgactc tcccttcctc tttgtatatc
    11401 cagatattgg taatgtgtct gcatggcata atgatatcta tagtgagttt tatcttggtc
    11461 atcatgccat cgcagctctc catctcaagg atacttatgc agtgacagaa agttcaaagg
    11521 gccagttccg agatgtacct ttcgggcaag gttgtgtcaa atgggaagaa gctttcgata
    11581 ttttaaagga aaccaattat aatggacctt tcctaatcga aatgtggtct gaaaattgtg
    11641 aaacagtaga agaaacacgc gcagccattc aagaggcgca agcttttctc tatccactca
    11701 ttaagaaagc aggtttgatg taagatgaat caagtaatca atgctatgcg taaacgagtc
    11761 tgtgatgcca atcaatcatt gccaaaacat ggacttgtca aatttacctg ggggaatgta
    11821 tctgaagtta atcgcgaact cggtgtcatt gttatcaaac catcaggcgt ggattatgac
    11881 gaattgacac ctgaaaacat ggtagtgact gatctagatg gtaagatcct agaaggggat
    11941 ttaagaccat cttccgacct cccaactcat gtgcaattat ataaggcttg gtcagaaatt
    12001 ggtagtgtgg ttcacaccca ttcgacagaa gctgttggtt gggctcaggc aggtcgtgat
    12061 attcctttct acggaacaac ccatgcagat tatttctacg gttcaatccc ttgcgcccgt
    12121 agtttgacca aggacgaagt agaagtggcc tatgaaaaag atactggcct ggttatcgta
    12181 gaagagtttg aacatcgcgg acttaacccg gttgaagtac caggaattgt tgtacgcaat
    12241 cacggtccat tcacctgggg caaaaatcca gagaatgctg tttatcactc tgtcgtacta
    12301 gaggaagtat caaagatgaa tcgctttaca gaacaaatca atccaagagt tggacctgct
    12361 ccccagtaca tactagaaaa acactaccaa cgtaaacatg gaccaaatgc ttattatggt
    12421 caaaagtaag aacgatgaag gaggagaaaa agataaattt agctcctctt tttacatttg
    12481 atttttattg agagtaaagt tggaattgaa gtaattttaa aagatttttt ag
//
LOCUS       DAVLFB010000055        12401 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLFB010000055 DAVLFB010000000
VERSION     DAVLFB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.055"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            114..278
                     /locus_tag="VXD58_001364"
     CDS             114..278
                     /locus_tag="VXD58_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV0676048.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            362..928
                     /gene="amaP"
                     /locus_tag="VXD58_001365"
     CDS             362..928
                     /gene="amaP"
                     /locus_tag="VXD58_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV0676049.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            940..1110
                     /locus_tag="VXD58_001366"
     CDS             940..1110
                     /locus_tag="VXD58_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV0676050.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1149..1757
                     /locus_tag="VXD58_001367"
     CDS             1149..1757
                     /locus_tag="VXD58_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV0676051.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1788..1991
                     /locus_tag="VXD58_001368"
     CDS             1788..1991
                     /locus_tag="VXD58_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV0676052.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2101..2379)
                     /locus_tag="VXD58_001369"
                     /pseudo
     CDS             complement(2101..2379)
                     /locus_tag="VXD58_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3143..3913)
                     /locus_tag="VXD58_001370"
     CDS             complement(3143..3913)
                     /locus_tag="VXD58_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV0676053.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3928..4776)
                     /locus_tag="VXD58_001371"
     CDS             complement(3928..4776)
                     /locus_tag="VXD58_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV0676054.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEDPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTGLDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4773..6248)
                     /locus_tag="VXD58_001372"
     CDS             complement(4773..6248)
                     /locus_tag="VXD58_001372"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV0676055.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVNFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6571..7926)
                     /locus_tag="VXD58_001373"
     CDS             complement(6571..7926)
                     /locus_tag="VXD58_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0676056.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLNQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWHAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7936..8253)
                     /locus_tag="VXD58_001374"
     CDS             complement(7936..8253)
                     /locus_tag="VXD58_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0676057.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8270..8587)
                     /locus_tag="VXD58_001375"
     CDS             complement(8270..8587)
                     /locus_tag="VXD58_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0676058.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8602..9498)
                     /locus_tag="VXD58_001376"
     CDS             complement(8602..9498)
                     /locus_tag="VXD58_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV0676059.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9501..10763)
                     /locus_tag="VXD58_001377"
     CDS             complement(9501..10763)
                     /locus_tag="VXD58_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0676060.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11043..12221
                     /locus_tag="VXD58_001378"
     CDS             11043..12221
                     /locus_tag="VXD58_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV0676061.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         4296 a         2459 c         1905 g         3741 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa
      121 tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta
      181 gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg
      241 tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc
      301 aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta
      361 tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt
      421 ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg
      481 gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt
      541 tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt
      601 ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg
      661 ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa
      721 tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat
      781 cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg
      841 tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa
      901 caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat
      961 cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt
     1021 ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta
     1081 tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg
     1141 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
     1201 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
     1261 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
     1321 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
     1381 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
     1441 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
     1501 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
     1561 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
     1621 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
     1681 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
     1741 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
     1801 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
     1861 aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagtcgccg
     1921 aagatgccaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
     1981 acgataaata aggttaaaag ttactttatc tttttagtaa cattagtcaa aagagtctga
     2041 gtcaagatga ttctcagaaa acaaaaagct agagattccc aattgcggaa ctctagcttt
     2101 ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga gtatgaatcc
     2161 catgtcaatt ctcacttgac gcttacctct cagatgacat ctcttataac ccaaacaaac
     2221 ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc
     2281 cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat
     2341 atacagtccc ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag
     2401 gctgtttgct ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaatagt
     2461 ttctatagta aaatgaacca aaaatagtac acgttgcggt ataatgttct tgtggcatat
     2521 tcattagatt ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat agtcaattga
     2581 ttgtcagttt gaagaccaag agtcgtaagg caaaggatat ggcagaatcc atccaagggt
     2641 ggctggcaca atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt
     2701 tttcaaaatg gaaagatatt tccaatcatc acgatagtga gagctttttt gcgaatctag
     2761 gatgtccaag atagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact
     2821 tgccaaagca gacagatttc aacgaagtca gtcaagaatt ttaggctggg caagaactca
     2881 atttcaggag aaaatgaaat aaatattccc acaataaaac gcatagtatc aaagtttttc
     2941 caacacctga tattatgcat tttttaattt taaagacttt ttcccagcct caagaaatca
     3001 catcccacct aaatcctttg gataccaaat acctcatcag gttattttct gagttggtaa
     3061 gactgtctag cttaatttga caaatggagg gcgttaaatc cagtttttac aggaagtcta
     3121 tttaaatatt ttcacactga ccttacaaaa tatgaaataa attatataaa gtcattccca
     3181 tgacaaagaa taacatccct ttaaaacagt atcttaccca attttcaggt aagacttttg
     3241 atacaaccgt ccccaatacc cctccgcata tagaagcaag aaaaataact ggaacaggtg
     3301 ctaaatgata ctgatttaaa cctgtaacca caacaattgt agccaaatga gacagctggg
     3361 aaaagaaaat aattgcaaga gaatacatcg ttgcttcttt tatcgaaata gaaaaaaaca
     3421 ccatcaataa agatacattg agcggacctc caccaatccc taaaaaacta gaaatcaagc
     3481 ccaatagaag accacaaatc atatagaatc ctatcttatc aaattgataa gtttttttaa
     3541 aatctgttaa aacaaatgat acaagtagca tcacaaatag taaaatcatt tgaacaatgg
     3601 atactagccg tactgataat tgagataaca aaacttgaaa aataagatga cctagaaatc
     3661 ctcctaaaac agaagaaaag gataccgtaa aaatcaatct ccattttatc tctttacttt
     3721 gtgaaaatct tttggttgtt gaaataatcg ccattataaa aacagagaaa ctagaataaa
     3781 aagcaatatc actgaccgaa tggtatccaa aactatccat aagtggctta attataattc
     3841 caccacccat gcctgatata gaacccaatc cactagctat tataatgatt agaaaataaa
     3901 taatctcaac cactttttaa gtccatccta ttctttcaaa catctaaaac ctagatggct
     3961 actagtggaa gtagaaatac atccatttct agcagctact cggtatcgat tacaataaga
     4021 acaatgacat aaaaaggaac ctcctctgat agcatactcc tcatcttgta tcccatattt
     4081 tggcaactca tagtcatctt cattaaaact agctaataag gtatatctcg gattgcgaca
     4141 ccattcccaa acattcccaa tcatttgata tagaccaaaa tcattaggta aaaattcata
     4201 gacaggagcc gttcctataa atccatctaa gccagtattt tcataaggaa atcttccttg
     4261 ccaagtattg gcatgataat atccaccttc taaaagagta tctccccaag gatattcaga
     4321 ttgtcttcct cctctagcag cgtattccca ctgtgcttca gtcggtaaag acattccgct
     4381 ccaattacag aaagccaatg catcttctaa agcaacatga actactggat ggtcttctaa
     4441 acccactaga ttactgttct caccgtaggg atgtttccag caagcgttcg gaacttgtaa
     4501 ccaccacgga gccccggcag gatgaggata tccctccctt tctgcctccg gaacgaacaa
     4561 tataaaaaca aaagaccact cttgtcgctc agctagtgtt ttatagcctg tttctttcac
     4621 aaattgagca aattcttgat tcgtaactgg agtatccgca atggaaaaat cctttacact
     4681 aacagcgact tgaggatctt caaaatcatc taaaaagccc tcttcagaat ttgttcccat
     4741 cataaatgtc ccacccttga tttttttcat tttcatgatt gactatcccc cgttttcgtt
     4801 agagttggcg ttattttaga aagttcaaca ggaactaact cttcatccac aacaaaacct
     4861 tcttctctat atctaagaaa gtttactaat tttttcttca tctgacgaac aataggttgg
     4921 tatttttcac ttggatacaa atcattcatt tcatgcggat cttttttcat atcaaataac
     4981 tgataatgat ttaaaacagg aaaccagata aatttccact ggtctgtcaa aatatattga
     5041 ctagaatctt ttcctaaagc atgttcacca tgaaattctg ttcgccaccc ctcgtactgt
     5101 ccaaataata aattcttaac actccgtcca tctagttcat ctgtagtagt tcctcctgcc
     5161 aaatccacga gtgaaggaaa aatatcttga attttcacta attgcttgat tgttccccta
     5221 ttaccagcta tcaatccagc cggatcgtaa ataaacgaag gaatgtggat acttccctga
     5281 taaggatatc cctttctaaa cagataatgt tcaccaagct gatctccatg atccgaaaca
     5341 aaccagataa tcgtatcttt atcatgacga aattctttta gggcagtcaa aaagcgacta
     5401 atttgatggt ctatatgagt aatcaatcca aaataagcag caaccattct tctttgatca
     5461 tcttccttta atttccctct taaagcataa atagacggaa tttgcttttc taatacttcc
     5521 caattcccga tatgcaaatc gagaaattga ggcaagcgtt ccatatacat atcaaaatag
     5581 tattttgggg gatttaatgg agcatgtggt ttttcaaaag acattttgag aaaaaaagga
     5641 accgttggat ctcttctttg taaaaatgaa atggactcac tcacaaccca gtttgttgga
     5701 tgtaactttt catctttatc ccaaggtcta gcttcccaag aattacaatc cattccgtca
     5761 tcaatcaaat caacatcata accaacttta ccctttaaaa aggctagata gtcactagca
     5821 taatcaaatt gacttccata agccttatca tactttctat caacatgtag atacccatcg
     5881 tgaagtaaga catgatcaaa cccaagtctc tgtctggaag gaaaaacatg catcttacca
     5941 atacactctg tttgataacc catatcttta aagacttttg gaagagtatt agtaaaattc
     6001 caaggcacct catcttgata accaacacgt ccacttttat cttggtctaa gccagtcaat
     6061 aaagctgccc tagcaggaac gcaagaagga actggagaat aggcattttc aaaattataa
     6121 cctacactag ccatcatatc taatgttgga gtgctaacta gcttatcctt actattcaag
     6181 gataaggcgt ctgctctcat ttgatctaca acaatcaaaa taatatttgg ctgttttgtc
     6241 tgaaccataa aatctccttt ctaatatggc aaaagaggca caagaagata tctaccttta
     6301 ctgcacccct ttctatatca atctctctat ataaaacaat aacattcttg ttatgtttta
     6361 tataaaaatg aactaaaata tgactaaatc gattaggaaa ttcaaatcat tttctagtac
     6421 tgttttagaa gttacagtgt actattccaa cttcaataaa ctataaacct ttgtctatta
     6481 acaattttag tggagataag aaatcctaca cctaactcat cttacacgta atctatttct
     6541 attttatcac aaaaaacgca agtaagacca ttaactcaat tcagttttat ctgccatttt
     6601 cacaaatggg aaataagtca agacactaat aatcaaacaa acaactgata agatgatggc
     6661 atgccaatca aatgctgtag agaagaaacc atataaaatt ggaggcatta cccaagtaac
     6721 attttgtgta acaggtgaaa caagacccca gcttgttgcc cagtaagcta ccgttgccat
     6781 gaaaaccggg ctaagtacaa atggtataaa tagcaaagga ttcaagacaa ctggtaaacc
     6841 ataattgacc ggctcaccaa tattaaacag aactggtgct agaccaagtt tagcaacttt
     6901 tcgataatga ctgtttcttg aaaaaattaa aatagcaagt actaatccta atcctccaaa
     6961 ccagacaaac gccccaaaag acccacttgt ccatatataa ggaatcggtt cacctttttg
     7021 gaaagcatcc agattcgcta acatagcaac tccaaatagc ccttccatga tgggagccaa
     7081 tacatttcct ccatggagac caaaaaacca gaataactta ttcaaaaaga tcatcagaat
     7141 aactgcaaag aaactttgag acaaacctag taatggcgtt tgtaacacct tgtaaaccca
     7201 atcaatcaat aagtcattgc taagtaaatg gaaaacataa gtcaagatgg ctactatata
     7261 catcgccata aatcctggaa tgatagaagt gaacggctta gcaatcgcag ggggaactga
     7321 atctggtaac ttgattaccc agttcttttt cattacttta cagaaaataa tagaggctaa
     7381 aaatccaatc atcatggctg taaagtatcc tctggcatta atatggtttc ctggaatcac
     7441 attcccaata gttaccatca gatttttacc atcaaatgct agattatcaa ttccttgtaa
     7501 gatttgattt aatttcacat ctcctacatt tgccaaaggg aaactctttg taactgtact
     7561 tccaatcgaa atgacaaacg aagcaagtga taccaaacca gcagaaactg tatcaacctt
     7621 gtaaatctta gcgatattca ctcccaagca atagatgaac aataaggaaa caattggtat
     7681 acttcccttg aataccaaat tattgatgtc aacaagccac tgaaaggttt tcgtaatact
     7741 ttctaggtga aattgttgtg gtaaatccac tagaaaagca tttaataaca aagcaatgga
     7801 acctgtcata ataacaggca tagtccccac aaatgaatca cgtaaagcaa ctaaaaactt
     7861 ctgattccca actttcattg caattggaga aagtttttct tgcattttat ccaacattgt
     7921 attcatagca aattcctaat ttttttcaaa tcgttcgtat acttctacca cttctttcgc
     7981 caagtcaaca aaggtcagac tcgtcattaa atgatcttgc gcgtgtacca tcaagaggct
     8041 aattgaagtt tcttccccct gtgcctctct tgtcaacatt tctgtttgag ccttatgcgc
     8101 ctgtaataag gcagcattcg catcagctaa tcttcgattg gctttagaga aatccccttt
     8161 ttttgctgcc tgaatagcct ccatagcatt acttttggct tccccaccat acataattag
     8221 ccccattgca gattctaaat tactttcatt catagcattc tcctcaatct tagtctaata
     8281 aactttcagc taaatctaag actttagaac cattcatcat tccataatct gtcatcggaa
     8341 taaccgccac aggaatctgt ctatctttta gtttttcttg aaagtcccct agtaaatagc
     8401 gaacttgagg gcctaaaagc aacacattta cttcctttgt tgctacaatt tcttctgctt
     8461 caggagctgg aactgcaaaa atttctgcat ccaacccctt atcttctgct gccttttgca
     8521 tctttgtcac tagcagactt gtactcatac ctgctgcaca tgctaacata attgttactt
     8581 tagccatagc ttactccttt atcacctatt tactgaccta gccaagcgag atactgtatg
     8641 tctcactcct ctaattgcct tgctcaatgc ataaatatta tcgactgttg ccagtccccc
     8701 atatcctgcg tcacctatat gttgaatatc aactccacaa attttatttc taagtgcaat
     8761 ttccttgata gtatctgtat cagatgtttc ttggctagta ccaatagcac tcagtactag
     8821 cccaccctta ctatgaacga gatcaacgac ttcacgcaac tcttggtcat gaaaggctgg
     8881 aacggttcca actgctggaa caagtatcac atccgcacct gcttccaaca attgctctgc
     8941 tacagaaagc tctgccacag gctcattcac tcctgccccg tgcatcttac ctgcaataat
     9001 caaaccagaa aagttttcct tagcagtttg aacagcctta atgatttctc gattgctaac
     9061 tcctgttcca ggatttccag tcaaacagac aaagtcaaaa cctaactcct ctattcgatt
     9121 caatgtttca acactagcaa cacgacctgc aacaatttcc tgtgtttcct ctagcatctt
     9181 tgcagatggg tcaatcggtt ccaaatttac accaattgga caagcaacaa gctggtgtaa
     9241 gcgatgaata acatcatcac ctgaactatc caaagcataa attttaggct caaaaacatc
     9301 aacacaattc aataaaatca tatcagctcc aaaagctcta gcaatttcag aattagttat
     9361 atccccaaca aatgtctcac gagtaactac attttcagat agaacaactc taccttcact
     9421 tgctaaaata ctttgtttta attcttcagc attcatctgt aatatctcag atggatttgc
     9481 actaattaaa cgtttcattc tcaatcctcc aataacttaa accgtttttc aatagctatt
     9541 acaaatggta aataaagtaa gacatccaaa acaatcaata caatttgtaa taatgaacca
     9601 gaaatacttc ctgtagcaag taaaccactt ataacaggtg gcatagtcca acctggatct
     9661 gtataagtta gcggaaccaa acctgatgcc attgctcccc acgctactag taagttaaac
     9721 attggagcta agataaatgg aactagtaac aaaatgttca aaacaatagg aacgccaaac
     9781 attgctggtt catttatatt aaataatcca ggtataacag ttataggtgc caaagttttt
     9841 agttgtttac tagctttctt cttatgtgct aaatatccta acgcaagaac taaacctatg
     9901 gttgcaccac cgccacctat gaatacaaaa ttatccataa atacgctagt aaaaatattt
     9961 ggaagagttt ctccagtttg ataagctact ttatttgcat ctagattagc taaccataac
    10021 ggtttcataa ctgcattaac aacatttcct ccgtgtaatc ctacaaacca aaaactacta
    10081 ttaaggaaaa ttaagataag aagaccaata acattatttc ctaaaagtcc aagtggacct
    10141 cccaaagcta cttgagcaat ttcatgaaga ttaggtaaac ttaatttatc taaagttgca
    10201 taaacaatca accaaccaac aatagtcact gcaccaggaa taatggcact aaagctttta
    10261 gatactgctg gtggaacacc atccggcatt tttatctgta tattatgatt tataaaccat
    10321 tggtagatat agccattaat caatcctaaa atcatagcaa caaataaacc ttttgatgcc
    10381 atgaaagcgg taggcattcc tgctccagcc tctcctctaa tgaaaggcgt aactgtaata
    10441 aaagatgata atgacacaat accagcagga ataccatcaa ctttgtattg tctagccata
    10501 aagtaagcga taccaaaact agctacaaga ccaagtaatc caaaactact atcaacaccc
    10561 ttccataaat agtcagcaac accaatatca cccagatatt tttcccatcc tggaatagga
    10621 aaacttgcaa taaccattag taatgaacct atcaacaata aaggtatagt tagagtaata
    10681 ccgtctcgaa tagaaaccaa cgctttatta ttacccaatc tagccgctac tggaagtaac
    10741 ttttcttgta ataatttttc cataaccttt tcctcttaaa tatattttat tatcaaaagt
    10801 tatcaattat ctttattttt tgacgtcaag ttgttatttg attatctttt gttatttata
    10861 ttttacaact ataagaaagc cttgtcaagg atttgtgtaa atatttgatt attttttatt
    10921 atcaatatat aataaaaaga gaccagtaag caatgaaaag cttaaaaaga ttaacatcta
    10981 catttatcca tagcaaaaaa tcataggatt ttaagaatct gacaactagg aagaaaacta
    11041 atatgacctt atctaagaaa caattacaac tacgagctaa aatactggaa acagtttaca
    11101 cattaggtcc catttcacga attgaaatcg ctactaaaac aggaataaca cctgcaacaa
    11161 ctagcagtat cacaaatgat ctgattaaag aaaacattct cttagaactt ggtgaagacg
    11221 aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga cattcaagct aaacgatttt
    11281 actacattgg ttgtgaacta tctgaaaaac atttcacttt tgctttagga gataatctag
    11341 gaaatatctt aaaggaagag aaagaaattg ttactaaaca gttaatccaa gaaaagggaa
    11401 atcaactcat tagccagaca ctaaagcaat tcttaaataa ctgctctgat tatgaaatag
    11461 aagcaattgg aatcgcgcta cctggtcgct atttagatga ttataaaata acaacaaaca
    11521 atcctttatg gcaacatatc gacttagaaa tgattcaatc ccactttgat aagcctttgt
    11581 ttttctctaa caatgttaac tgtatggcta taggtaaacg cctatttagt cgccaacaga
    11641 atgaccctaa ttttgcttat tttcattttg ctagaggtat gcactgttct tatatctacg
    11701 acggaaacat ttatggaaaa ggaaatctaa tgattggaga aatagggcat acagttgttt
    11761 catctgaggg agaagaatgt agttgcgggc gcaagggatg cttacaaact tttgctggag
    11821 aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca atctccctat tctctacttc
    11881 ctagtttagt caaaaatgcc gatgatattg atatccaagt tattttaacc gcttatcaat
    11941 taggtgacac tggaataatc acacttatcc atcaagcctt actctatctt agtcaaacca
    12001 ttttaaatat cagtatgatg attgactctc aaaaaatcta cctacatagt cctttactaa
    12061 caaatcaaca catcatccaa aaactatata gtgaaatgaa ctacaaaccc aaactgctct
    12121 ataatcgcct acctgaagtc attattgaac cttacaatga ttttacagcc gcacactcag
    12181 ctattgcact ttgtttgtat catacaattc tacattccta attttctatt acatttaata
    12241 aataggctaa aaagagggag tatatcataa aaagcgaaca aacctgtttt ctgaaaatca
    12301 gaattctgtt ttgttcgctt tctttatatt tggaattata ctcctatctc ccctttttca
    12361 taatgtatag taaaatgaaa taataacagg acgaatcgat c
//
LOCUS       DAVLFB010000056        12379 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLFB010000056 DAVLFB010000000
VERSION     DAVLFB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.056"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..1144)
                     /locus_tag="VXD58_001379"
     CDS             complement(<1..1144)
                     /locus_tag="VXD58_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV0676062.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENVEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQIN"
     gene            1202..2368
                     /locus_tag="VXD58_001380"
     CDS             1202..2368
                     /locus_tag="VXD58_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV0676063.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            2507..2833
                     /locus_tag="VXD58_001381"
     CDS             2507..2833
                     /locus_tag="VXD58_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0676064.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            2820..3413
                     /locus_tag="VXD58_001382"
     CDS             2820..3413
                     /locus_tag="VXD58_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV0676065.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3406..4317
                     /locus_tag="VXD58_001383"
     CDS             3406..4317
                     /locus_tag="VXD58_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV0676066.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4380..5444
                     /locus_tag="VXD58_001384"
     CDS             4380..5444
                     /locus_tag="VXD58_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676067.1"
                     /translation="MKQEWFESNNFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAILIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5516..6376)
                     /locus_tag="VXD58_001385"
     CDS             complement(5516..6376)
                     /locus_tag="VXD58_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV0676068.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDDSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLAQEK"
     gene            complement(6498..7484)
                     /locus_tag="VXD58_001386"
     CDS             complement(6498..7484)
                     /locus_tag="VXD58_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV0676069.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7756..9231)
                     /locus_tag="VXD58_001387"
     CDS             complement(7756..9231)
                     /locus_tag="VXD58_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV0676070.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9489..10412)
                     /locus_tag="VXD58_001388"
     CDS             complement(9489..10412)
                     /locus_tag="VXD58_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0676071.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10426..11355)
                     /locus_tag="VXD58_001389"
     CDS             complement(10426..11355)
                     /locus_tag="VXD58_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0676072.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11674..11895)
                     /locus_tag="VXD58_001390"
     CDS             complement(11674..11895)
                     /locus_tag="VXD58_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676073.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(11849..12109)
                     /locus_tag="VXD58_001391"
     CDS             complement(11849..12109)
                     /locus_tag="VXD58_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676074.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         3685 a         2633 c         2279 g         3782 t
ORIGIN      
        1 tatttatttg gcttatttta tccaaataaa ttattgttct cttgtactcc ttcttccatt
       61 taaatcctct aattaaacct acaatcgtcc caaggccata agcaaagtga atggaaaata
      121 aaagaaaggg catcacaatt agaaatccat ttttatgttt taataaagtc agcaaagtga
      181 gtaatgacaa aagtagaaaa taggcaccta atagtaaagt tatgaatacg aatgtgatcg
      241 gtaacaatgc tagactaaac acaagactca aaacaaataa acaaggaaca tagtgaaata
      301 atgataaaca cttaaactga acatgacttg tcaagccaat ccacaaacca tttgaatact
      361 tttgatgcag cattttcttg aatgttggtc gaatatactg ataagataga atacttgggc
      421 tatagcggat tttataacca tgttctcgaa ttctataatg aatatcatta tcttcagttc
      481 ggccaagttg ctcatttact aaaccaacct tctggaaaac ctctcgttta tacatcccat
      541 gaaaaataga agaaacatat ctatcctcgg aactatttcg ataattggca atgctactgc
      601 caaacatatt ttcctcaaca agatgcaagg tctctgccca ttttcctttt ccttcgacaa
      661 tcgtcggtct aggcccccca cagacaaatt caccttgttg aataatagcc acattgttca
      721 ttacaaaact ctcagtaact tttgaatgag catcaatttt taaaataagg tcccctacag
      781 aatgtttaac tcccaggtta aaaccactag cttgattttt cttaggattg ttatacaatc
      841 taattgagtt aaactctgta tcttccttta taaattgctg aatgatagct gtggtcccat
      901 ctgtggacat agcatttata aatagaattt caacattctc tttaggatag gtttgatttt
      961 ttaagtcttc aattagacca ggcagatatt tttcttcatt gtatgcactg attacaatgg
     1021 atacgagaag actttcaaca ttttgagtca ctactactct cctgaatttt tccttccacc
     1081 attctatcat aattttcaag actttcccat ttttatcttg aaagccagaa accttacttg
     1141 acattatagt gaaaaagcct taaaataagc tgttattaaa atcagctaga agaggtatta
     1201 tatgaaaaag caatcactct tttttgttcc aggtattatc ctgattggtg tttccttgcg
     1261 aactcctttt actgttttac ccattatttt gggaaatatt tcgcaaggtc tggaggtaga
     1321 agttagttcg cttggtgtct tgaccagcct gcctctcctt atgtttaccc tcttttcacc
     1381 attttctacc cgactggctc agaaaatcgg cttggagcat ctcttcacct acagcctctt
     1441 cttcttgacc atcggctcac tcattcgact aatcaatctg cccctgctct atctaggaac
     1501 cttgatggtt ggggcaagtg tcgcagtcat caatgtcctg cttcctagtc ttatccaagc
     1561 caatcaacca aagaaaattg gttttctgac caccttatat gtaacgtcta tggggattgc
     1621 aacggctctg gcttcctatc tagctgtgcc cattacacaa gccagttctt ggaaaggact
     1681 tatcctcctc ctcacgttac tctgtctagc aacttttttg gtctggctcc caaatcaccg
     1741 ctataatcat agactagctc cacaaaccaa acaaaaaagt caaataaagg tcatgcgtaa
     1801 taaacaggtt tgggcaatta ttatcttttc aggttttcaa tccttgatct tttacaccgt
     1861 catgacctgg ttacctacca tgtctatcca tgcaggtcta tccagtcacg aagctggctt
     1921 gctgacttct atcttatctc tgattagcat tcctttttca atgaccatcc caagcctgac
     1981 aaccagttta tctactcgca accgtcagct catgctcact ctggtttcac tagctggtgt
     2041 ggtcggcatt tccatgctct ttttcccaat caataatttc atttactggc ttaccatcca
     2101 tctcctcatc ggaaccgcaa ccagcgccct cttcccttat ctcatggtca acttttcact
     2161 caagacaagc gcccctgaaa agacagccca attgtccggc ctatctcaaa caggaggcta
     2221 tatcctagca gcctttgggc caaccctctt tggttacagt tttgacctgt tccactcttg
     2281 ggtaccagct gtagctgccc tcttgctcat ctatatcctg atgactgtgg ccctctttac
     2341 agtggacaga gcggataaga tcctttaaac ttctagtttt agctagaagt tttttatata
     2401 taaaaatttt acacatttct cttgacaggg ctttctttta gatgtacaat gtatgtgtag
     2461 aaaaattata tataaaaatc ttacacatta gaaaaggagg tttcccatgt actttccaac
     2521 atcctctgcc ttgattgaat ttctcatctt ggctgtactg gagcagggtg attcttatgg
     2581 ttatgagatt agccaaacca ttaagctgat cgctaatatc aaagaatcca cactctatcc
     2641 cattctcaaa aaattggaag gcaatagctt tctgacaacc tattctagag agttccaagg
     2701 tcgcatgcgc aaatactact ccttgacaaa cggtggtata gagcagctct tgaccctaaa
     2761 agatgaatgg acactctata cagacaccat caatggcatc atagaaggga gtatccgcca
     2821 tgacaagaac tgaatacctg actcagctag aactctatct caagaaacta cctgaagctg
     2881 accgtatcga agccatggac tatttcagag agctctttga cgatgctgga gtcgaaggag
     2941 aagaagaact catcgctagt ttgggaactc ccaaagaagc ggcccacgaa gttctatcca
     3001 atcttctcga taaaaaaatc aatgaagcac ccgctcaaaa aaataaccga caaattttac
     3061 atatcgcctt gttagccctc cttgcagcac ctatcggcat tcctctggga atcgccatcc
     3121 tcgtgaccct gttcgcaatc cttgtagccg ctttgactgt cattctggct ttctttgcag
     3181 tttccatact gggtatcatc ggcggattcc tatttttagt tgaaagtttc actgtcctcg
     3241 cccaagccaa atcagccttt atcttgattt ttggttctgg tttactggct atcggtgctt
     3301 cttcgctagt tttacttggc atttcctatg taactcgctt cttcggtcta ctcattgttc
     3361 gtctggtaca atttgttctt aaaaaaggaa agagaggtaa ccagcatgcg taaatggaca
     3421 aaaggatttc tcatctttgg tgtggtgact accgttatcg gctttatcct gctttttgta
     3481 ggtatccaat ctgacgggat taagagccta ctttccatgt ccaaagaacc tgtctatgat
     3541 agccgtacgg aaaagctaac ctttggcaag gaagtcgaaa acctagaaat tactctccac
     3601 caacacacgc tcaccatcac agactctttc gatgatcaaa tccacatttc ttaccatcca
     3661 tctctttctg ctcaccatga tcttatcacc aatcagaacg atagaactct gagtctcact
     3721 gataagaaac tgtctgaaac tccgtttctc tcttctggaa ttggtgggat tcttcatatc
     3781 gcaagtagct actctagtcg ttttgaagaa gttattctcc gactaccaaa agggagaact
     3841 ctaaaaggga tcaacatctc agccaatcgc ggacaaacca ccatcataaa tgctagcctt
     3901 gaaaatgcga ccctcaatac aaacagctat atcctccgaa ttgaaggaag tcgtatcaaa
     3961 aacagtaaac tcacaacacc caatatcgtt aatatctttg atacagttct tacagatagt
     4021 cagctagagt caacagagaa tcacttccac gctgaaaata tccaagtcca tggtaaggtt
     4081 gaactgactg ccaaagatta tctcagaatc atcctagacc agaaagaaag ccaacgaatt
     4141 aactgggaca tctcaagcaa ctatggttct atcttccaat tcacaagaga aaagcctgaa
     4201 tcaagaggta cggaattaag caacccttac aaaactgaaa aaaccgatgt caaggatcaa
     4261 ctcattgcga gatctgatga taatattgat ctaatatcca caccaagcag acgttgacaa
     4321 aaacgcttac atctgctact atggaagcat atttatctaa ctaaaaagga ggttctacca
     4381 tgaaacaaga atggtttgaa agtaataatt ttgtaaaaac aacaagcaag aacaagcctg
     4441 aagagcaagc tcaagaggtt gcagacaagg ctgaagaaac gatagccgat ttcgatacac
     4501 caattgaaaa aaatactcag ttagaggagg aagtctctca agctgaagtc gaactggaaa
     4561 gccagcaaga agagaaaatt gaaactcctg aaggcggtga agcgagaaca gaaatagaag
     4621 aaaagaaggc atctaattct actgaagaag agccagacct ttctaaagaa acagaaaaag
     4681 tcactatagc tgaagagagc caagaagctc ttcctcagca aaaagcaacc acgaaagagc
     4741 cacttcttat cagtaaatct ttagaaagtc cttatatccc cgaccaagct ccaaaatcta
     4801 gggataaatg gaaagagcaa gtgcttgatt tttggtcttg gctagtggaa gcgatcaaat
     4861 ctcctacaag taagttggaa acaagtatca cacacagtta cacagccttt ctcttgctca
     4921 ttctgttttc tgcatcttcc tttttcttta gtatctatca catcaaacat gcttactatg
     4981 gacatatagc aagcattaac agtcgcttcc ctgagcagct agctccttta actctttttt
     5041 ctatcatctc tatcctagta gcgacaacac tcttcttctt ttcattcctc ttgggtagtt
     5101 tcgttgtgag acgatttatc caccaggaaa aggactggac gctagacaag gttctccaac
     5161 aatatagtca actcttggca attctaatct tcctcactgc tattgctagt ttctttgctt
     5221 tctttgatag cctacgattt acagctctct tgtgtgtgat tagcattgga atcattctgc
     5281 ttgccagtct ccatatcatt acaagaccaa gtcaagcaag tgaaaccgac tccttctatc
     5341 aattattctt gtctgtcctt gtgaacggag ttattatcct cctcttcttt gtagctgaag
     5401 tggcactgat tggagattat cttcgtatct tggcctttct ttaaaattca atcctgtcat
     5461 ccttggcagg attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact
     5521 tttcttgtgc taatgtttta aaatcttgta ctaggatggg ttgtatttct atttgattgg
     5581 cgtctagttg gtggtaaaaa gatgggggta atacctctaa gaattttgca tagtccaagc
     5641 gagctatgga ttcatagtct tctggtttag agtcgtctcc tgtgatttga accaagtcaa
     5701 tctcccactg gacttcctta tccaccaatt gctcaatata ggctgcagga acaaacggtt
     5761 ctctcggtaa aactgtcttg actccttgag ctagatggta aacgccatgc acttttcctt
     5821 ctccatcctg atagaaggaa actctcgcaa agtaggcatc tgtctcttga accgcacgca
     5881 cacgcgcttc aaactgagcc aagccatctg cctctaaaaa gcttttcaag ttctcacgac
     5941 taaagaaaat aggattaaaa attccttggc aaatcgtaaa acgagctaca gggtctgcta
     6001 gataggcttg aatacttgct tggaaataag cttcaaaatc aaaaccatct agcccttcaa
     6061 tatcagtccc tgtataagca agcaaagcat ctaaaaatga cttgataatc tgccagtctg
     6121 tctttgttag taggtcagga agatcgacac ggtaagcctt ttgaccatca gcataactga
     6181 ccttaaaaag aaattgtgat tgccccacta tcgcacactc aatatactcg agacgattaa
     6241 gaggctgacg gagatagact acatcatagc tatgtgactc caaaccgtct agcaaggcca
     6301 agatcgactt ggcattcaaa atctcttgtt ctcctaaaat gctctgttta tttggaataa
     6361 aaaatgtttt cgccataact ggactccttt gaaatcgttt acatacagta taccttattt
     6421 ttctgaaaga tacaaaaaca aactttagat ttttgagtac aaagcataga aaaacagccc
     6481 ctgaggactg tttgatctta tacagtagct gcctgatcca agctttcacc gatagcggct
     6541 aggcgctcaa tcacttcagc ttgtgtcaat tcattttttg aaacatagcg gttacgtggg
     6601 tgaacacggc actcgtgtga gcatccacga aggtacttgt cttcattttc ttctgatgtc
     6661 aagatacgac ggttacagaa gggatttcca cagttgacat aacgttcaca tggtgttcca
     6721 tcaaaccagt ctttccctac gatggttggg ttgacatggt tgacatcaac ggcgatacgc
     6781 tcgtcaaata cgtacatttt tccatcccaa agctcacctt gaacttctgg gtctttaccg
     6841 taagttgcga ttcctccgtg caattggccg acatctttgt agccttcacg gaccatccag
     6901 cctgagaatt tctcacagcg aacgccacct gtacagtaaa ccacgacacg cttgtccatg
     6961 aatttttcct tgttatcacg gacccattgt ggtaactcac ggaagttgcg aatatctggg
     7021 cgaatagctc cacggaaatg tcctaggtcg tactcataat cgttacgtgt gtcaaggaca
     7081 acggtatctt tatcaagaag cgcttctttg aactcttttg gagacaagta agcacctgtt
     7141 gtttcaagtg ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag gtggacaatt
     7201 tctttcttgt agcgaacaaa catcttcttg aaggcttgtt cattttcttc gtcaatcttg
     7261 aaccagagtt cttccattcc tggaaggctg tgaacgtagt ccatgtattt ttgagttgtt
     7321 tcatagtcac ctgaaactgt tccgttaatt ccctcgtcag cgactaggat acggccttta
     7381 aggccgattg atttacagaa agccaagtgg tctgcagcaa attgctctgc attttcaatt
     7441 ggagtataaa ggtagtaaag taagacacga atatcttttg ccataagatt tgttctcttt
     7501 tctattctta aattttctaa attttttatt tatctacact agtatacctg cttgagaaaa
     7561 cgaatgcaat ttatttgact ggaaattgta gagggttctc gaaatttcct gaatggttaa
     7621 aataaggaca agagaaaaca tggatatcta tatccttgtg ccaaaaaaac cactgccctc
     7681 cccagaccaa cctgaggaaa gcagtgattc ttattttagg agttaggaat gaatacacga
     7741 aatcaattta gctgattatt ttttgttttt caagaattca tcgtattgtt tttgcatttc
     7801 gttcaatact ttttcgtagg caccttcaga tttcaatttt tccatcaatt ctggaatcgc
     7861 tttatctggg tctacagtac cagtgttgat agctgtatca aattgttgca ttgtgttagc
     7921 aatagctgag atttcagatt tcacattgtc agtattgaag ataaatccaa gcgctggaga
     7981 ttctttagct tctgccaatt ctttcttaga attttcgatt tgttggtctg taacgttttc
     8041 gttgatgtaa aggatccagt tgttaccagt gttccatcca cccatgtgag tgtttccttt
     8101 gtagccatca agaacgcgaa cacggttttc tttaccttca attttttccc agttcttgcc
     8161 ttctggaccg taaacaagac cgttcaagag ttctggattc gtattcaaga ggttcaagat
     8221 ttccattgat ttttctttgt tcttagagtt gtttgagatg acaaagttag caacttgtgt
     8281 tgtttggttt ttcttgatga agttagtaat tggtttgatt tggatatctt tgttggcaac
     8341 acgtgaaagc aagctgttac cgtagtcagc tggtcctact gtttcttcac gaacgaacca
     8401 agtatcttgt tgaaggtcaa aggaagtatc gcttgttgcg acatcttttg gaatgtagcc
     8461 agcttcatag aatttgtgaa gagtcttcaa gtgttctttg aaacgaggca cttcgtaacg
     8521 gtttacaact ttagtagtat cgccttcaag gtcaataacg aatggaagcc cgtttgctac
     8581 tgggtagtca aagttatcag atgggatgaa aactttacca atagcaaatg gtactacgtc
     8641 tggagctttt tctttaattt gtttcaagac tggctcaaga gtttcgtaag aagtaacacc
     8701 tgaaatatcg ataccatatt tggcaaggag agttccgttg aaggcaaagt tttgagatga
     8761 tgcaacgttg gctgcaactg gaacagcgta aattttaccg tttacagtat tacccttgat
     8821 gtaagctggg tcaagtgcct tgtaaaggtc tttaccttct tttttgtaca attctgtcaa
     8881 gtcagcataa gcaccttttt gagcatttac aatatagtta tctgcaaagg cgatatcata
     8941 gttttcacca gatgatgtga taactgacat tttcttacca tagtcacccc agccaaggta
     9001 ttggatatcc aatttagcac caactttttc ttcgatgatt ttgttggcat ttgctaacaa
     9061 ttcatccaag ttatctggtt tgtcaccgat ttggtacatt ttgataacag gtttgtcacc
     9121 tgaatcagca gcttttttgc tgttacctgt caagttgcca caagcagcaa gacctgcagc
     9181 cagagcgact acactagcag atgcaaaagc atattttttc cagtttttca tgataaaaac
     9241 tccttttttt atttttaaac ttataaacaa tgtaatgatc ttatactcaa tgaaaatcaa
     9301 agagcaaact agaaaactag ccgcaggctg ctcaaagcac tgttttgagg ttgtagataa
     9361 gactgacgaa gtcagttaca tatatctacg gcaaggcgac gttgacgcgg tttgaatttg
     9421 attttcgaag agtattaact tcacacaagg gaagttggga actgagaaat gttctttttc
     9481 aataagcact attctttcac accaccgata gtcaaacctt ttacaaagta gcgttggaag
     9541 aatggatata aaatcgcgat tggaagggtt gcaaccacaa ccatggccat acgacctgtt
     9601 tctttcggta gagcaactcc cagttgacca gtcaagccga ccgctttggc aatgtagtcc
     9661 atattttgtt ggatttgcat gagcaaatat tgcaatggat acaagttatc actcttgatg
     9721 taaagaaggg cgttgaacca gtcattccag aaaccaagag ctgtcaaaag cgtgatggtt
     9781 gcgatacctg gtagtgacaa tggcaaacag atttggaaga aaatccgggc ctcactggca
     9841 ccatcgatac gagccgattc gagaatggct tctggaatgg tcttcttgaa gaaggaacgc
     9901 atcaagatga tattgaatgg tgagagaagc attggaacaa tcaaggccca aacagtgtcc
     9961 ccaagctgaa gtacacgggt caccatgata taacctggta ccaaaccagc gttgaacaac
    10021 atactgagaa ggacgaagat ggtaaaaaat ctgcgatact taaaggttgt ccgtgaaata
    10081 gcgtaggcat aggttgttgt gataaagaca tttgtcaatg tcccaactac ggttacaaag
    10141 acagagatga agagggcttg taggatttta tccttaaact gtgccaaaaa ctcaaaaccg
    10201 tctaagccaa attgggatgg gaagaagcta tagccgtatt ggaggaggct tttctcgtct
    10261 gtcactgaaa taatgataac gaatacaaaa ggtaggatac aagagagggc aatcaaaccc
    10321 gaaatgatac tgaagaagat atctgctttc ttactgaagg agtgaatgcc gacattatca
    10381 attttttctt ttttaatttt cttttttgcc atattctcct cctttctaga acaaagctga
    10441 gtttggatcg actcgtcttg caagcaagtt tgataggata accagaatca aaccaacaac
    10501 ggattggtaa agaccggctg ctgcagccat accgatatct gctgtctgag tcaaaccatt
    10561 aaagacatat acgtccaaaa cgttggttac attgtaaagc tgaccagcat tgtgtgggat
    10621 ttgatagaag agaccgaagt ctgcgcggaa gatatttccg actgcaagga tggtcaatac
    10681 agttacaagc ggagtcaact gaggaatggt tacgttgcga atacgttgcc acttgctagc
    10741 tccgtccact gtcgctgctt cgtagtaggt tggatcaatt cccatgatcg tcgcatagta
    10801 catgacactg ctatatccaa atcctttcca aatacctagg aaaagtagga gatagggcca
    10861 gatgcccagg tcagcgtaga aattgacttc tttgagacca agactttcca atagatgatt
    10921 gaacacccct ttatcaatat ttaggaaggc atctgtaaag aaactgatga taacccaaga
    10981 caagaagtaa gggaacaaca tagaagtttg aaaaatcttc accattctct tagaacggag
    11041 ctcgctgagg ataatggcga tccctacaga tacaactaaa cctagaaaga taaagccaag
    11101 attgtagagg acagtatttc gtgtgataat aaaggcgtct cttgaactaa ataagaatct
    11161 aaaattatcg agtccgaccc atttactatt tatgatacta tctatgaaac cattactggt
    11221 catgtggtag tctttgaagg caaccacgtt cccaaatact ggaatgtaaa agaatagaat
    11281 caaccagagt gcccctggca aaaccatcaa gagaaagatc cagttgtctc tcaatgtttt
    11341 tgaaaacttt ttcataattt cctccctttt tattttgata tccatctaaa aattcttttt
    11401 tagacttttg ataacgatta cattattagt atactcctat ttgcaggtta ggttaaactc
    11461 ctaattatag aaaaaactcc acaaattatg tagcagattt aaaactttat caccactatc
    11521 aaacaaatgt cctaaatcaa ttgtttattt tatctctatt agcccagtga tggcgtcact
    11581 ctgttataag catccaacaa cggggtatac tgaaaaatct ccagactagg gaactcagcg
    11641 atagttccta atctggagat ttttaatatg ttattaggcg tttgctttca acttagcaat
    11701 aacctcttta agattatcaa tcaactctgc tgcagtatgc tcagagcctt tttcatctgc
    11761 caagaacaaa actgcttttt gaagttcttt ttgagagttt tcaaggacat ccttatctac
    11821 tgtttcaagg tttgagtctt taagaagttt acttaattcc ttggctaatt tcttgagttt
    11881 gatttgcaga ctcatcttct cctgctgttt ctttgcccgc tgtttgtcct ccatccttag
    11941 ttgctgactg gctttcctta atggactcta gggaagcaat ggcatctttg actgtttgca
    12001 agatatcacg taaaccttgc tctgtcaaac tatcatctgt aagagctttc ttagcctctg
    12061 ccaaaaccag acgtgctgaa tctgtggtag gattcgatac acctgtcaat gatctcaaaa
    12121 gattttctaa ggtttgagtc tgcttactaa tactagacta aaatcaaaaa gcattacata
    12181 acagtgatat gaaatcaact aaagaagaaa tccaaaccat caaaacactt ttaaaagact
    12241 ctcgtacagc taaatatcat aaacgccttc aaatcgttct atagtaaaat gaaacaagaa
    12301 caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga agtagatgtg
    12361 tactgttcta gtttcaatc
//
LOCUS       DAVLFB010000057        12346 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLFB010000057 DAVLFB010000000
VERSION     DAVLFB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.057"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            123..314
                     /locus_tag="VXD58_001392"
     CDS             123..314
                     /locus_tag="VXD58_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676075.1"
                     /translation="MYFRFFSNFDDFLEKNYLKTIWQVSKETPFSEMDFNMFKNISEK
                     IIFERGSKMLNDLKSNYKK"
     gene            452..1105
                     /locus_tag="VXD58_001393"
     CDS             452..1105
                     /locus_tag="VXD58_001393"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV0676076.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            1115..1579
                     /locus_tag="VXD58_001394"
     CDS             1115..1579
                     /locus_tag="VXD58_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV0676077.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1592..2860
                     /locus_tag="VXD58_001395"
     CDS             1592..2860
                     /locus_tag="VXD58_001395"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV0676078.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKFNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2874..3134
                     /locus_tag="VXD58_001396"
     CDS             2874..3134
                     /locus_tag="VXD58_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676079.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            3145..4506
                     /gene="pdrM"
                     /locus_tag="VXD58_001397"
     CDS             3145..4506
                     /gene="pdrM"
                     /locus_tag="VXD58_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV0676080.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4660..5628
                     /locus_tag="VXD58_001398"
     CDS             4660..5628
                     /locus_tag="VXD58_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV0676081.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5644..6636
                     /locus_tag="VXD58_001399"
     CDS             5644..6636
                     /locus_tag="VXD58_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV0676082.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6854..7897
                     /locus_tag="VXD58_001400"
     CDS             6854..7897
                     /locus_tag="VXD58_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV0676083.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7961..9646
                     /gene="lpdA"
                     /locus_tag="VXD58_001401"
     CDS             7961..9646
                     /gene="lpdA"
                     /locus_tag="VXD58_001401"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV0676084.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9710..10699
                     /locus_tag="VXD58_001402"
     CDS             9710..10699
                     /locus_tag="VXD58_001402"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV0676085.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10772..11842)
                     /gene="xerS"
                     /locus_tag="VXD58_001403"
     CDS             complement(10772..11842)
                     /gene="xerS"
                     /locus_tag="VXD58_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV0676086.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3701 a         2274 c         2711 g         3660 t
ORIGIN      
        1 ttcctcatga cttttttgat atatacctag atcatgtagc aaaatatgcc tacgaacaaa
       61 aagttaataa tattaaagag tattatcctt taaaaagagc gattttacac caatagaatg
      121 cattgtattt tcgatttttt tctaattttg acgacttttt agaaaaaaat tatttaaaga
      181 ctatatggca agtttctaaa gaaactcctt tttctgaaat ggattttaat atgtttaaaa
      241 atatttcaga aaagataatc ttcgaaagag gtagtaaaat gcttaatgat ttaaaaagta
      301 attataaaaa atagccagtc tttataaact gtattcacgt attcaaatcg tatatatagt
      361 cgatttatta aatctttaga taaactgtaa aaagtggttt ccatagccac tttttgctat
      421 aatagaggca gtaaaaatga aggagtcggc tatggaacac tcgtcttggc atgctttgat
      481 taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat cagtttatgg agcaggtcta
      541 ttctcagggg attatttatc cacccaagga aaaggttttt caggctctct tgacaacact
      601 gcttgaagaa gttaaggtgg taattctagg gcaagacccc tatcacggac caggtcaagc
      661 gcagggcttg agtttttctg tacctgactc tatcccagct ccaccatcct tgcaaaatat
      721 cttgaaagaa ttgtcagatg atatcggagt taagaaatct catgatttga cagcttgggc
      781 tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt cctgctggac aggccaatgg
      841 tcatgctggt caaatctggg agccttttac tgatgctgtg attcaggtgg tcaatcatct
      901 agatagacca gtcgtttttg tactctgggg agcttatgca cgtaagaaga aggcattagt
      961 taccaatcct catcacttga ttatcgaatc agcccatcca agtcctttgt cggtttatag
     1021 aggattttgg ggttccaagc ccttttccaa ggccaatgca ttcttaaaag agacaggaca
     1081 agagccaatc gattggctta gataaggaga aaatatgcct cagttagcga cgatttgcta
     1141 cattgataat gggaaagaac tgctcatgct ccatcgtaat aagaaaccca atgatgtcca
     1201 tgaagggaaa tggattggtg tgggtggtaa gctagagaga ggagagacgc cccaggaatg
     1261 cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc aagccagttc taaaaggtgt
     1321 catcactttt cctgaattta caccagattt agactggtac acctatgttt ttaaggtgac
     1381 ggagtttgag ggcgacttga ttgactgcaa tgaggggacg ctagaatggg ttccctatga
     1441 tgaggttttg agcaagccga cttgggaagg tgaccacacc tttgttgagt ggcttttaga
     1501 ggataaaccc ttcttttcag ccaagtttgt ttatgatggg gataaattgt tggataccca
     1561 agttgatttc tatgaataaa ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat
     1621 ggatcccaag tctggtttgg atcaagtttg tgatgtctta gttcaagatg ggaaaattat
     1681 caaaattgcg cctgagatca cggaagaagg agcagaaacg attgatgcta ctggtcttgt
     1741 ggttgctcct ggtttggtcg atattcatgt gcatttccgt gaacctggtc aaacacataa
     1801 agaagacatt catactggtg ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat
     1861 gatggctaat accagtccaa ccatttcaga cgtggagact ttgcaagcag ttctccagtc
     1921 agctgccaaa gagaagatta atgtcaagac agttgcgacc attactaaaa actttaatgg
     1981 taaaaacttg actgacttta aggcgctctt agaagctggt gcggctggtt tctctgatga
     2041 cggtattccg cttgagagca gtaagattgt caaggaagcc atggaggaag ccaaaaaatt
     2101 caataccttt atcagccttc atgaggaaga tccaggtttg aacggtgttc ttggctttaa
     2161 tgaaaatatt gctagagaac atttccatat ctgcggtgct actggagtgg ctgagtacgc
     2221 tatgatggcg cgtgatgtca tgattgccta tgcaactaaa gcccatgttc acatccagca
     2281 tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct caggggttag gtgcagaagt
     2341 cacagcagaa gtagcgccac agcatttctc taagacagaa gcacttcttt taacacaagg
     2401 tagcaatgct aagatgaatc caccgcttcg tttggaatca gaccgtcgtg ccgttatcga
     2461 aggtctcaaa tcaggtgtca tcacagttat tgcgactgac cacgcgcctc atcatgtaga
     2521 tgaaaaaaat gttgaggata ttaccaaagc gccatctggt atgactggct tagaaacatc
     2581 cctgtctctc agcttgacct atttagtaga agctggtgag ttgagcttga tggaattact
     2641 tgaaaaaatg acatacaacc cagccaagct ttacaacttt gaagcaggtt acttggctga
     2701 gaatggtcca gcagatatca ctatttttga tgccaaggct gaccgctttg tggactccca
     2761 ttttgcttcc aaagcagcta attcaccatt catcggtgaa accttaaaag ggcaggttaa
     2821 atataccatc tgtaagggac aaatcgtcta tcaagcttga taggagttag aatatgaatt
     2881 attttcgaaa acgtagggag agacaagcga aatctaatag tggaatttac tgtcccgcag
     2941 ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg
     3001 ataacggagg tttagttaga agctattatt gtcgtacatg tgcgtgttgg catactactt
     3061 caaaatctaa taaacctccg ctaagtttga aaaactatgg tctgaaactc tttggtttag
     3121 ataaatcaga atagaaagag aagtatgtat aagacaaagt gtttacgaga gaaattagta
     3181 ttatttttaa aaattttctt cccaatcctg atctaccaat ttgccaatta ttctgcctct
     3241 tttgttgata ctgcaatgac aggtcaatac aacactatgg acttggctgg tgtatctatg
     3301 gcaaccagta tctggaatcc tttctttaca tttctaacag ggattgtgtc agccttggtg
     3361 cctatcattg gtcaccatct tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac
     3421 caatttattt atttggcctt gggcctatct gtggtcttgc tggggatggt acttttcttg
     3481 gcaccaataa tcttgaatca tattgggtta gaagcagcag tagcggcagt agcggttcgc
     3541 tatctttggt ttttatctat cgggattatc cccttgttgc tctttagcgt cattcgttcc
     3601 ttgctggatt cgctgggctt gaccaaactg tccatgtacc tcatgctttt gttactccct
     3661 ctcaatagcg gatttaacta tctcttgatt tacggtgcct ttggtgttcc agaactggga
     3721 ggggctggtg ctggtttagg aacatccttg gcctactggg tcttgcttgg gatttctgtt
     3781 ctggttttat ttaaacagga gaagctcaaa gccttacacc ttgagaaacg aattccactt
     3841 aatatggata acattaagga aggagttcgt ttaggtctgc ctattggggg aactgtcttc
     3901 gcggaagtgg ctatcttttc agtggttggc ttgattatgg ctaagttttc gtccttgatt
     3961 atagctagtc accagtcagc tatgaacttt tcaagtctta tgtacgcctt tcctatgagt
     4021 atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat
     4081 gcgaaaacct atattggtct aggaagatgg actgctctca tttttgcggc cttcacctta
     4141 accttccttt acatttttag gggaaatgtg gccagtcttt atggtaacga cccaaaattt
     4201 atcgatttga cagtgcgttt tttaacttat agtcttttct tccagttagc agataccttt
     4261 gcggcgccgc ttcagggaat tttgcggggg tataaggata cagttattcc tttttacctt
     4321 ggtttgcttg gttattgggg cgtagcaatc cctgtggcta cgctatttga ttccctaaca
     4381 gattttggag cttattctta ctggatcggc ttgattatta gtttgattgt gagcggggcg
     4441 ctctaccgtt ggcgtttaac tgtgattatg aagagatttg aatctttagc aaaatccaaa
     4501 tgctaaaaag tttgtgaaaa aatattcttg acaagaaaat cccttgtttt cacggggttt
     4561 tcttttttat tttgtgaaat ttcttttaga aaaactttga cagtatttgc caaaagcggt
     4621 aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga tgtcaacttt agataaaaat
     4681 cttttgctag aaatgttccg taagatggaa gaaatccgtc gcatggactt aaaaattgca
     4741 caattagtaa agaaagggaa agtgccagga atgacgcact tttctgttgg tgaagaggca
     4801 gctaacgtgg gggctatgtt agctctcaat ccagatgatc tgattacctc aaaccaccgt
     4861 ggacacgggc aagctattgc taaagggatt gacctcaacg gaatgatggc tgaaatcctt
     4921 ggtaaataca ctggaacctg taaagggaaa ggtggatcta tgcatatcgc tgaccttgat
     4981 gctgggaacc ttggtgccaa tggtatcgta ggtggtggta tggggatcgc tgtcggtgca
     5041 gccctcagtc agcaaatgca aaataccggt aaaatcgttg tctgcttctt tggagatggt
     5101 gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg cttctatctg gaacctgcca
     5161 gtcattttct attgcattaa caacggttac ggtatctctg cggatatcaa gaaaatgacc
     5221 aatatagaac atatccatca acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa
     5281 gacggtaaca atgtcatcga tgtctatgaa ggatttcaga aagctgtgga ccatgttcgc
     5341 agtggcaatg gtccagtctt gattgaaagt gtaacttatc gctggctcgg tcactcatca
     5401 tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca
     5461 atcgaaaacc tccgcaatta ccttattgaa aataacattg caagtgccga agaattggaa
     5521 gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg ttaaatttgc agaggaaagc
     5581 ccattcccac cgcttgaatc agcctttgaa gatatttacg cagactaagg agaaagagat
     5641 aaaatggaaa caaaaacaat gtccttccgt gacaccatta tccttgctat gtctgaggaa
     5701 atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg tcggtgtctt cggaggagac
     5761 ttcggtactt ctgttggaat gcttgaagaa tttggtccag aacgtgtccg tgactgtccg
     5821 atttctgaag ctgccatctc tggagcagca gcaggagcag ccatgacagg acttcgtcca
     5881 atcgtcgata tgaccttcat ggacttctct gttattgcca tggacaatat cgtcaaccaa
     5941 gctgctaaaa cacgttacat gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt
     6001 gcagctggta acggagttgg ctctgccgcc cagcactcac aatctctaga gtcttggttt
     6061 actcacattc caggacttaa ggttgtggca cctggaacac ctgcggacat gaaaggcctg
     6121 cttaagtctt ctatccgtga taacaactca gttattatac ttgagtacaa gtcagaattt
     6181 aaccaaaaag gggaagtgcc agttgatcca gactacacaa ttccacttgg ggttggtgaa
     6241 atcaaacgcc aaggaacgga tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg
     6301 gttcaagctg ctgaagaatt agcagaagaa ggaatttcag ttgaaattgt tgacccacgt
     6361 acccttgttc cgcttgataa ggatatcatc ataaactcag tgaagaaaac tggtaaggtt
     6421 gttctggtca acgatgccca caaaacaagc ggctatatcg gtgaaatttc agctattatt
     6481 tcagaatcag aagcatttga ctatctagat gcaccaatcc gccgttgtgc aggagaagat
     6541 gtgccgatgc cttatgcaca aaacctagaa aatgcaatga ttccaacagt tgaaagtatc
     6601 aaagatgcaa tccgaaaaac ttataacaaa gaatagggtt acataagata agttatgtaa
     6661 ataaaataaa gctgacgaga aactttgtat cttttaggtg cagagctctt cgtcattaat
     6721 atcttagatt agagcacggg ctaaaagatc ggaaaaaaga taaatctcct tggcttcatc
     6781 gaagccatcg tcgatttcct atttttcagt cgcttttgac gccctttgta tcataaatag
     6841 aattggagag gtcatggctg atgacaagct aagagcgact cctgcagcta gaaagttagc
     6901 ggatgattta gggataaacc tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca
     6961 caaagaagac gtggaaactt ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa
     7021 acgaattgcc ctcgaacata acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg
     7081 taaaatcatg aagaaggatg ttttggccct gcttcctgaa aatatcgaaa acgatagcat
     7141 caaatctcct gctcagattg aaaaagtgga agaagtccct gataacgtaa caccatatgg
     7201 taaaatcgag cgtattccaa tgacaccaat gcgtaaggtt attgcccaac gcatggttga
     7261 atcttacttg actgcgccaa ccttcaccct caactatgaa gttgatatga ctgaaatgtt
     7321 ggctcttcgt aagaaggttc ttgagccaat catggaagca actgggaaga agactactgt
     7381 aacagacctt ctttcgcttg cagttgttaa gactcttatg aaacacccat acatcaacgc
     7441 ttcattgaca gaagatggca agactattat cactcacaac tatgtcaatc ttgccatggc
     7501 agttgggatg gataatggat tgatgacacc tgttgtttac aatgctgaga agatgagtct
     7561 ttcagaactg gttgtagcct ttaaggatgt tattggccgt accttggatg gtaaattggc
     7621 tccaagtgag ttacaaaatt caacattcac aatcagtaat ttgggaatgt ttggtgttca
     7681 gtcctttggt ccgattatta accaacccaa ctcagctatc cttggtgtca gttcgacaat
     7741 cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc ccaatcatga gtttaggatt
     7801 aaccattgac caccgtgtcg tagatggtat ggctggtgct aagtttatga aggacttgaa
     7861 agagttgatt gaaacgccaa tctcaatgtt aatttaagag caagagtatt catttttaag
     7921 atatagataa aggaaggaag acatggcctt agaagtaatt atgccaaaag ccggcgtgga
     7981 tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc ggagaatttg taaaagaagg
     8041 agaaatcctt ttggaaatca tgactgataa agtcagcatg gaattggaag ccgaagaaga
     8101 tgggtacttg attgccattc tcaaaggaga tggtgaaact gtccctgtaa cggaagttat
     8161 cggttacctt ggtgaagaaa gggaaaacat cccaacagct ggagcagcat cgccagaagc
     8221 tagctctgta cctgtagcaa gtacctcaaa cgatgatgat aagagcgatg atgcttttga
     8281 tatcgttgtg attggtggag gtcctgctgg ttatgttgca gccattaaag ctgcccaatt
     8341 cggcggtaag gttgcccttg ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg
     8401 atgtattcca accaagacct accttcataa cgctgaaatt attgaaaata tcggtcatgc
     8461 tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact gttgatatgg aaaaactttt
     8521 agaaactaaa tctaaagttg ttaatactct tgttggtggt gttgcaggac ttcttcgtag
     8581 ctacggagtt actgttcata aaggaattgg tacaatcact aaagacaaga acgtcttggt
     8641 aaatggttct gaattgcttg aaaccaagaa aatcattctt gctggtggtt caaaagtcag
     8701 caagatcaac gtccctggta tggaatctcc acttgtcatg actagtgatg acattcttga
     8761 aatgaacgaa gtgccagaaa gccttgttat catcggtggt ggagttgtcg gtatcgaact
     8821 cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt atcgaaatga tggaccgtat
     8881 agttccagct atggatgtgg aagtttctaa gaaccttcgc ttgattcttg agcgtaaagg
     8941 aatgaccatc ttgactggta ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg
     9001 tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa gctcttcttt caattggtcg
     9061 tatgccagac cttgaaggta ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa
     9121 ggtcaacgaa tacatggaaa cttcagttcc aggcatttat gcaccaggtg acatcaacgg
     9181 tactaagatg ttggctcacg cagctttccg catgggtgaa gtttccgctg aaaatgccct
     9241 taaaggaaat catgcagttg ccaaattgaa tttgactcct gcagccatct acactctccc
     9301 tgaagtagca gcagtaggtt tgacagaaga acaagcccgt gagaaatacg atgttgccat
     9361 cggtaagttt aactttgctg ctaacggtcg tgctattgca tctgacgcag ctcaaggttt
     9421 cgtaaaagtt atcgctgata agaaatacgg agaaatcctt ggtgtacaca ttattggtcc
     9481 tgcagccgca gaattaataa acgaagcatc aagcatcatc gaaatggaaa tcactgttga
     9541 ggaaatgctg aagaccatcc acgggcaccc aacttactct gaagtgatgt acgaagcgtt
     9601 tgcagatgtt ctaggaatgg ccatccattc acctaagaaa aaataaaacg aagatagatt
     9661 agactggaag gttatttcca gtttctatca tataaagtaa agggatatca tgaaatacat
     9721 tatcaatcat tcaaatgaca ctgcttttaa tattgccttg gaagaatacg cctttaaaca
     9781 ccttttggat gaggatcaaa tcttcctact ttggattaac aagccatcta tcattgttgg
     9841 tcgtcaccag aacactatcg aagaaatcaa ccgtgattat gttcgcgaaa atggtattga
     9901 ggtagttcgc cgtattagtg gtggtggagc tgtttaccac gatttaaata acctcaacta
     9961 cacgatcatc tccaaagaag atgaaaataa ggcatttgac ttcaagagct tctcaactcc
    10021 ggttatcaat accctggctc aacttggggt taaagctgag ttcacaggtc gtaatgacct
    10081 tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat
    10141 gcaccacggt tgcttgctct ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt
    10201 ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat
    10261 tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc cgtgatttac tcttggaata
    10321 catgaaaaaa gagtacccag agatgactga atacgtcttt tcagaagaag aattggccga
    10381 aatcaatcgc atcaaggata ctaagtttgg aacttgggac tggaactatg gtaaatcacc
    10441 tgaatttaac gtccgtcgtg gaatcaaatt cactagtggt aaggttgaag tctttgctaa
    10501 cgttactgaa tcaaaaatcc aagacatcaa gatttatggt gacttctttg gtattgaaga
    10561 cgttgctgca gtagaagatg tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa
    10621 ggcgctaaaa accattgata tcacacgcta cttcgctggt attagccgtg aagaaatcgc
    10681 tgaagcggta gttggataaa tcaaaaagaa gttggccaac taaccaactt ctttttttga
    10741 tatttattta cataatttaa aatacgtaaa atcataaact atccagagca ttcttttgtt
    10801 catcattaac aatatgggta tagaggtcag tgacttgtgt gctggcatgt cctagttggt
    10861 gactgactaa aacttgtgat ttagtagcat catagagcct agttgctagt gtatggcgca
    10921 gtttatgggg tgttacacgt actttaaaat cctctgagta tttagcaacc attttctcaa
    10981 cgctagaagc atcgatacga ttaggaacac ctctgtagag agttaaaaaa agggctgtat
    11041 ctgttttttc cgttttatag cgttgattcc gaatggccag ataattctct aaataaggtt
    11101 tagcaaaagc agcgacattg actgagtcac gtttgccacc ttttcgagta acatcaataa
    11161 ccatcatttt tagattgaga tctcttagat ctagattaac agcttcagat aagcgaacac
    11221 cagatgccaa gagaagggca ataatggcta aatctcgttc tttatttttg ttgaatgatg
    11281 agagagctcg atttgaaagc tgttgtgggt gctcttgatc gatataagtt agaaaacctt
    11341 ctgtttcatc acctagaaag agtttttgct tgatattttc agctctggca gcaagggttt
    11401 ctttcttttt cttggttgaa acttttttca ttacattacg atagaaataa ggttccccct
    11461 gatcgttttc aacctcctcg gttagatact tgtaaagact agaaagtgct gataaggttc
    11521 gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt agcattcagc aagggacgtt
    11581 cacgtagata aaggataaag gattccatgt ctttcttaga catattttcc aaaactgata
    11641 aaggaatatc ggatatttta tcagcgtttg aaataccaga ctccaaaacc cagctgaaaa
    11701 atcggtcata ttccttaagg tattcgtaca gggttgtaaa actgtagggc acagccagtt
    11761 tagattggta gtattccaga acataccagg gcatgagttg ttttagtttg tcgattcgtt
    11821 ccagtaaaat ctcacgtttc atgtattttc tccataatta agtatactag tagtatagca
    11881 tagacttgta tatttttcaa gaaaattata gtttttcaga aagatgttat aggagttttt
    11941 caaagtgata aaaagactta gacctacaaa caaaacagaa taaggaaaat aataaaaatc
    12001 tcacccacta aacttctgtt aatactacat aactgaagag ctatataagg agctatgaga
    12061 tacaaaacac attgtataat agattgaaac tagaatagta catctggact tctaaaacat
    12121 tgctagaaat tgatttgatt gtcctgattg ttttatccta tttctatttc atttctctat
    12181 ataattgcta ttgttttctt tttcatgatt ttaatcttac tgtacagatt tgatacagta
    12241 aactcctttc tagctttata ctttatcatt tctaacttaa ttatagtctt atttttaata
    12301 aagttcaatt atttatatgt aaaattcctc atcagtaaaa aataac
//
LOCUS       DAVLFB010000058        12310 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLFB010000058 DAVLFB010000000
VERSION     DAVLFB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.058"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(26..223)
                     /gene="blpI"
                     /locus_tag="VXD58_001404"
     CDS             complement(26..223)
                     /gene="blpI"
                     /locus_tag="VXD58_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV0676087.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            505..1092
                     /locus_tag="VXD58_001405"
     CDS             505..1092
                     /locus_tag="VXD58_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV0676088.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1019..2662
                     /locus_tag="VXD58_001406"
     CDS             1019..2662
                     /locus_tag="VXD58_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV0676089.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2673..4034
                     /locus_tag="VXD58_001407"
     CDS             2673..4034
                     /locus_tag="VXD58_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV0676090.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4091..4219
                     /gene="blpC"
                     /locus_tag="VXD58_001408"
     CDS             4091..4219
                     /gene="blpC"
                     /locus_tag="VXD58_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV0676091.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4290..5630)
                     /locus_tag="VXD58_001409"
     CDS             complement(4290..5630)
                     /locus_tag="VXD58_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0676092.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5644..6381)
                     /locus_tag="VXD58_001410"
     CDS             complement(5644..6381)
                     /locus_tag="VXD58_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0676093.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6386..6724)
                     /locus_tag="VXD58_001411"
     CDS             complement(6386..6724)
                     /locus_tag="VXD58_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0676094.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7000..7329
                     /locus_tag="VXD58_001412"
     CDS             7000..7329
                     /locus_tag="VXD58_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV0676095.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7497..8546)
                     /locus_tag="VXD58_001413"
     CDS             complement(7497..8546)
                     /locus_tag="VXD58_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676096.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8543..9274)
                     /locus_tag="VXD58_001414"
     CDS             complement(8543..9274)
                     /locus_tag="VXD58_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676097.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9342..9752
                     /locus_tag="VXD58_001415"
     CDS             9342..9752
                     /locus_tag="VXD58_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV0676098.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9762..10049
                     /locus_tag="VXD58_001416"
     CDS             9762..10049
                     /locus_tag="VXD58_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676099.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10211..10495
                     /locus_tag="VXD58_001417"
     CDS             10211..10495
                     /locus_tag="VXD58_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676100.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(10807..11943)
                     /gene="dnaJ"
                     /locus_tag="VXD58_001418"
     CDS             complement(10807..11943)
                     /gene="dnaJ"
                     /locus_tag="VXD58_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV0676101.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         3973 a         2658 c         2180 g         3499 t
ORIGIN      
        1 aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa
       61 taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa
      121 tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt
      181 cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt
      241 tcttttttta tttttgtctt gttgtaactt tgataagttt agtatatcat cgttttttaa
      301 aatttttcat ccagatcttg aattgtcatc gaaacgtctt gaattaggtt ttttatttca
      361 agccacctct aaatgtttaa aaaaaataat ttctaatcac ttttttacca ttcaggaagt
      421 tttaatgact attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta
      481 tccactatat gaaaggaatt gccaatgact tcttataaac gtacatttgt tcctcaaata
      541 gatgcgagag actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat
      601 ttttctctag ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt
      661 ggcattgtaa aagccgctga tgaaatgggc tttgaaacaa gacctgttca agcagataaa
      721 acgctctttg acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga
      781 aaactccaac attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat
      841 cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact
      901 ggagtagcta tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat
      961 ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat
     1021 tgttctctca agcttattgg tcactattat caatataggt ggttcttact atctccaagg
     1081 aatcttggat gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg
     1141 tctggttatc acctatatcc tccaacaagt catgagcttc tccagagatt atctcctaac
     1201 cgttctgagt cagagattaa gtattgatgt gattttatcc tatattcgcc atatttttga
     1261 acttcccatg tctttctttg cgacacgtcg tacaggagaa atcatttcac gattcacaga
     1321 tgctaactct attatagatg ccttggcttc taccattctt tctctttttc tggatgtttc
     1381 tattctgatt cttgtaggag gcgtcttact ggcacaaaac cctaatctct tccttctttc
     1441 tcttatttcc attcctatat acatgttcat catcttttct tttatgaaac ctttcgaaaa
     1501 aatgaaccat gatgtcatgc aaagtaattc tatggttagc tctgccatta tcgaagatat
     1561 caacgggatt gaaactataa agtcgctcac gagtgaagaa aatcgctatc aaaatataga
     1621 cagcgaattt gtagattatt tggaaaaatc ctttaagctc agtaaatatt ctattttaca
     1681 aacgagttta aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc
     1741 tcaattagtc atgtcgagta aaatttctat cggtcagctg attaccttta acacactttt
     1801 ttcttacttt acaactccta tggaaaatat tatcaacctc caaaccaaac tccaatctgc
     1861 gaaggtcgct aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca
     1921 agaaaaccct gtccattcac attttttgat gggcgatatt gaatttgatg acctttctta
     1981 taagtatggt tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga
     2041 taaggttagc ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt
     2101 caatttcttt gaaccctaca aaggacatat ttctatcaat catcaggata ttaaaaacat
     2161 tgataaaaaa gtcttgcgcc gtcatattaa ttacctacca caacaagcct atatctttaa
     2221 tggctctatc ttggaaaact taactttggg cggtaatcat atgattagcc aagaagatat
     2281 tctaagagct tgtgaattag ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta
     2341 tcaaactcag ctctctgatg gagctggtct atcaggagga caaaagcaac gaatagccct
     2401 cgctcgtgct cttttaacta aagctcctgt tttaatacta gacgaagcta ctagcggtct
     2461 tgatgtcttg actgagaaaa aggttataga taatcttatg tctctaactg ataaaaccat
     2521 tctctttgta gcccatcgtc tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga
     2581 ccaggggaaa atcattgaag ttggtagtca ccaagagtta atgcaggcgc aaggtttcta
     2641 ccatcatcta ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg
     2701 agttttatca gagacgttac cataactatg cgacagtgtt cattatacct ctttcattac
     2761 tatttacttt catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc
     2821 aaggagaaat cgcccctaca agtgtcattg cctccattca gtcaaccagt gataatccta
     2881 tcctagctaa tcatttagtg gcaaatcaag tagttgaaaa aggggactta ctcatcaaat
     2941 actctgaaac aatggaagaa agtcagaaaa ctgccttagc aactcaatta caaagacttg
     3001 agaagcaaaa agaaggactt ggaattttga aacaaagctt agaaaaagcg actgatcttt
     3061 tttctggcga ggatgaattt ggctaccata atacctttat gaattttact aaacaatccc
     3121 atgatattga actgggtatc acaaagacta acaccgaagt ttcaaatcaa gctaatcttt
     3181 ccaatagcag ttcatcagct attgaacaag aaattacaaa agttcaacaa caaattggag
     3241 aatatcaaga gttgagagat gctatcataa ataacagagc acgcttacca actggcaatc
     3301 cgcaccagtc aattttgaat cgttatcttg tagcctcaca aggacaaaca caaggaactg
     3361 cagaggagcc atttttatct caaattaatc aaagtattgc aggtcttgaa tcatctatcg
     3421 caagcctcaa aattcagcaa gctggtatcg gaagtgtagc aacttatgat aacagtttag
     3481 caaccaaaat tgaagtactc cgcactcagt ttttacagac agcctcacag caacaactaa
     3541 ctgtggagaa tcaattaaca gaattaaaag tacaactaga tcaagccaca cagcgtttgg
     3601 aaaacaatac cttaacctcc ccaagtaaag gtatcgttca tctgaacagc gaatttgaag
     3661 gtaaaaatag aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata
     3721 caagagaagt actaatcact tactacgtat cttctgacta tctacctcta ctagataaag
     3781 gacaaactgt aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc
     3841 aacttcagac aattgatcaa actcctacca gaacagagca aggaaatctc tttaaattaa
     3901 ccgctcttgc aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc
     3961 gcgtcactag tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa
     4021 cccattctga ttaattttca gataacactc tataactatt tattatctta tcaaaaagga
     4081 gaatcataac atggataaga aacaaaacct aacttcattt caagaactaa caactaccga
     4141 actcaaccaa attacaggtg gaggattgtg ggaagattta ttatataaca ttaatagata
     4201 tgctcattac atcacataag aacttcatca tccaatacaa ctataaaaaa ataagaccga
     4261 gaaacaagta ctctcggtct tatttttcat cattctgtat gtatcacagt aagtacctga
     4321 cgaaagactt gattttgaca ggtggtattt agactggtat taggatggct ttccacaatc
     4381 ttcatgacgg tatagagacc aactcctctc tcctcccctt tagaactggc tccaaaggag
     4441 aagatttcag aaatatcgat gccctcttct ttgatggagt tttcgatgat aaaggtctcc
     4501 tgtgctccat tttttaaaaa ggcgattgaa acatgaggtt gactagcttc cacactggct
     4561 tcaatagcat tgtcacaaag gatagacaca atggttagaa aatcaagtag actcatcccc
     4621 tcgacctgaa tctcctcagg aacttcgaca ttaaagacaa tgttcttatc tctggctttt
     4681 aaaaatttcc ctgctagaag acttttgagg gctttatcac gaatatttac caatctgccc
     4741 aggtcatatt tattgttctg caatttctga ctggaatcct ttaagacgga gccatagacc
     4801 tcttttatct gctccatatc ctcctcttca atgcccagac gtaagctagt caagaggttg
     4861 gtataatcat gacgaaagct ccgtacttcc ttgtaaagct cctctatatg ccgactatag
     4921 cgttccatat ctctatagcg cagggcctgc tcttgttcca atctctcata gagtttttcc
     4981 ttcaaatagg tatccaattt cttgataacc cccataaaaa agagtaggta aaagactagg
     5041 atgagatggc gaacagtctt tgattgaata ctttgttcat attcaaaaaa agacagactt
     5101 tccatgacta gatagtagcc acccattatc cagttaatct gagtcaggga cttttgaaag
     5161 gctttatcga gaatctcctt tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa
     5221 gctagagaaa tgaagaaatt gaaaattatt atacataacc cagtaaatga gtagccatca
     5281 tatacttgcc cttgtcccaa aaatggaagc acaaaatagg agactcctct ataaaagaga
     5341 ttcaccaata tcattggaaa gagaccataa aagaaaagga gttttttagg aagccctctc
     5401 aataataaga aagataagcc tatgccgtac aagggttcca taaaataaga taggtaaaca
     5461 tttcctacta tatagctaat catcacaaaa acaaaggcca acagtatctt aaaaagaaag
     5521 gccttaaaaa tcctctcgaa agtaagatca attccatcca ccttaaagaa gatgacaatt
     5581 tctagtccat tagtaacaag tgtatacaac aatatccaag caatgttcat aaattctcct
     5641 agctcagtgt aatttattga tggcctcaga cacttccctg accttataac gggcgattag
     5701 acaacttcca ccattgggaa agaaaagcag tttttctttc ttatccaaat gcaccacatt
     5761 tgcaggattg atgagaaaag agcggtggca ctgcaagaga cggggctcct gcttgaaaac
     5821 ctcctctaaa ctcgctgtaa attccagcct gtctgtcttg gtatagagaa taacacgatg
     5881 ggctctgggc gacgtttcga gatagtaaac ctctttaaaa ggatactgaa attgggcaaa
     5941 ttttgattta aagtaaaagc aatcttccgc cagactttta ctatcttgac tattggcata
     6001 gaggagggct gtctcgatcc gagattcaaa ctcctctgct gacaaggcct tatcaatgta
     6061 gtccaaagca gacacttggt agcgaaaaga caggggcata aactccgagt gagtcgtcac
     6121 aaagacaatc agggcataag gatcccgatc ccgaatcttt ctagccactt ccagtccctt
     6181 catctcttca tttcgaatct caatatccaa aaagaatagc tgatgggccc ccttctcatg
     6241 cacttcagcc agcagttggt ccggcttgcc aaatacctca aaagagctag gaatgatatg
     6301 atgtgctttc aaaagtttct caatcgtcgt ttcaattcta gtctgttggg aaaaatcatc
     6361 ctctaaaaca aatattctca tcttcttact ctccctcttt caaccatttt tgacgaattt
     6421 ctctatagcg acgtctggca tacttgacag cgtattgacc ttcttctccg agaaaaagga
     6481 ttctttcttg aaaatcaatc gattttaatt tatcaagatt aaccaaacaa tttcgatgac
     6541 atctcatcaa ggtttcccca cattggttct caagattact taaattttga agcatattaa
     6601 aactagcttc ttctgtaaca atctgtacgg tatgggcttt agttggatgt gtttggatat
     6661 agtagatatc gtcgatgttt actttataga ctgttttctg cgtctgaata atcatatatt
     6721 tcattggatt ctcctcctaa gaaaagaata acagaaaagg tctagcccaa gcacacccaa
     6781 tcctaaacag tctttgaaaa ctcctaaaag gattattttc tacaacatca tgatgagctt
     6841 acaactattt tatcataaaa ttcactcagc gcaattcaag acatttcaat gacaattaaa
     6901 gatttgaata aaaaaaaaga attaaaagtg ataaactaac agtatcaaaa cacagttgcc
     6961 agaagtaaga ctggcaccca gattaaagga ggcattccca tgacagatac agaccccatc
     7021 aaaagagctc atactttgat tactgactta aacaaagcct accaagcatg caaacaggca
     7081 agcgctgacg acgtccgctt tcaggagcaa ttaaactcta ttcttggttt tctagccaag
     7141 gctgaaacag tggataatcg attcttgatt gaactagaaa aattttacca gacttccagt
     7201 cttctcatgg gactcagcgc tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc
     7261 tatgaccgct tccactttga ccaagtcaag accaagttaa tactcaatga aaatcaaaga
     7321 gcaaactagg aagctagccg caagctgtac ttgagtacgg taagacgacg ctgacgtggt
     7381 ttgaagagat tttcgaagag tataagtctc tacggaccaa ccattatcct gtagtaacaa
     7441 aaaagaagtt gagacagact gaactcaact tcttttttag tgtcgtatca gcaatgttag
     7501 tcctgcatct gacgttttac ctgataaggc aaatacaaga ctagtaaaac caaaccagct
     7561 cctagtaagg ctagaagggc tagtttttct tggaaggtaa tcaacccaac aactaattcc
     7621 acaagtaaaa caaaactggt caatcctcga actacctcct gtaagccttt ttccttttgc
     7681 cccttgtcca gcggaaagag ttgggtcaaa tactggtagt caaaggcatg atagagggcc
     7741 agcaactgga agagcaagag gtagttaaag agaactacca ctgctgtcgc aatccaagct
     7801 tgctcgataa aaacctgcgc cagtacggaa agtaagagaa gacgaagact gagagcaaag
     7861 aggtcgccat ttcgcagata agaacgcaga tagagatttt gccaaatctt tccagacacc
     7921 ttctgaacag cctttaagat aaagtccaga taggcacgac gtttgacact gtttgaaatt
     7981 ccctttacct gcgtaaagag ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct
     8041 tgggaaatga catagtccca gtccagccca gtttcagtga aaaatttgct ggccttttga
     8101 cgaaagtgga aatattttcc tacccccaat aaaagcacat agagcagaaa aactggcaag
     8161 ccataaccca ttgctaaaaa taaaggcgca aataacagca agaaaagggt ctgtacaaag
     8221 agccaaaaga ctagggaaat gccagtttga cgcttgagat ggagcttgat ttcctcttct
     8281 ccaactaaga gaaagagctt gtctggagcc tccatatagg tggcaattcc tccccaaagt
     8341 aaaagtaaaa cagacgtaat tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa
     8401 tgttgtaaga gttgactgta ctggtaggct agaaagccca acaggacaag caggaacaag
     8461 acaaagtggt cattgagcac atagcgcaga taaccaaggc actccttacg aaaggcctgc
     8521 tttctcttta aaaacaagtc tttcatagat cctcctcttt ggtcagagcc aagtaaatat
     8581 cattcaaact agcctcaggc atatcaaagg cttcacgtag ttgcaggaga ttgcctttgg
     8641 cacgcacctc tcccttgtga agaatgacaa aggcatcaca catcttctcc gccgaatcca
     8701 gcacgtgggt actcatgaga atagacttgc ccttttgctt ctccacttcc aaaagctgaa
     8761 tcaaatcaga aatagccagc ggatcaagac caaggaaagg ctcatccacg atgaaaagac
     8821 ttggatccac cacaaaagca cagataatca tgaccttctg cttcatccct tttgaaaaat
     8881 gaacagggaa ccagtctaat ttctgttcca aacggaacat ttttaacaag ggctctactc
     8941 gttcgaaagc cactttttgc tcaataccgt aagccatagc aaccgtttcg atatgctctc
     9001 tgagggtcaa ttcctcatac agactaggcg tctcaggaat gtagccaatc tgcttgcggt
     9061 agctagtcgc atctccttgc agagtcaggc cattgatatt gatggagcca ctataaggtg
     9121 ccaacagacc gataatctca ttgatcgtcg ttgatttccc agcaccattg agaccaatca
     9181 aaccgaccaa ctgcccactt tcaacagtaa aggacacatc tttcaaaaca gggacatgaa
     9241 catagccacc tgtcaggttt ttaatttcta acatattttc tccaaatctg gtataatgta
     9301 gctatattat atcaaaattc agtacagtag aggtagattt tatgtcagat tgcatttttt
     9361 gtaaaatcat cgcaggggaa attcctgctt caaaagtata tgaagatgag caggtccttg
     9421 cctttcttga tatctctcaa gtaacactag gacacacctt ggtcgtgcca aaagaacact
     9481 atcgcaatct tttggagatg gatgctacga gcgccagcca actctttgcc caagtaccaa
     9541 aagtagctca aaaagtcatg aaagtcacta aggctgctgg tatgaatatc atttccaact
     9601 gtgaagaagt cgctggtcaa acagtttttc atactcacgt tcaccttgta cctcgctaca
     9661 gtgctgacga tgacctcaag attgatttta tcgcccacga accagacttt gacaaacttg
     9721 ctcaagtcgc tgaaaccatc aaaaacgctt aaggagttgc tatgaaatta tccaacctac
     9781 tgctatttgc aggagctgca gccggaagtt atctggtcac aaaaaatcgc caaaccatca
     9841 cagatgaagt cttgaatacc actgaccgcg ttcaagctat caaggacgat gtggatatta
     9901 tccaaaacag cctgcaaatc attgaccagc aaaaagaact tatcaaggaa taccaagaag
     9961 acttgactta caagtttaag gtcttggaaa aggatatcca gactagacta gctgtgataa
    10021 aagaaatgca gggaactgaa gataagtaaa aagagccccc cggctctttt ttaaaacgta
    10081 aacaagttat agtgaattga atttagaata gcacatcata gtttctaaag cattgttaga
    10141 gattacttta aaatcctctt atcaatttgt tcatattcta tttcaatcta ctatatatat
    10201 cccatcaact atgcatcata atttaggtaa ctcatactca ataaaaatca aaaagcaaac
    10261 tagaaagcta ggcacagact gctcaaaaca ccgttttaag gttgtggata gaactgacga
    10321 agtcagctca aaacactgtt ttgaggttgt agatacgact gacgaagtca gctcaaaaca
    10381 ctgttttgag gttgtggata gaactgacga agtcagtaac catacccatg acaagccgac
    10441 gctgacgtgg tttgaagaaa tttttgaaga gtatcattca ccatttcaca actaaatcga
    10501 agactttctg ccgtaatgaa aacacctgaa acagcttggt ttcggctgtc cggaaacttt
    10561 aagacttagg ttcaaagttt aggtatggaa cttcgaagga ggtcgctacc gtccgtcatt
    10621 acttaggaaa gtcttaaaaa atcggctcta taaagtaaaa agagcccgac agctcttttt
    10681 gtttatattc aatgaaaatc aaaaagcaaa ctaggaaact agccacgggc tgctcaaaac
    10741 actgttttga ggttgtagat acgacaagcc gacgctgacg tggtttgaag aaatttttga
    10801 agagtattat tctccatcaa aggcatcttt aatatggtca aagaagcctt ttttctttgg
    10861 atttactttc aagtcaccag cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt
    10921 caagcctgtc ggtgttacga cattaacagt aacgtattgg tcaccaactg caccgccacg
    10981 aaggctcggt gcccccttac tacgtaggcg gaatttctta ccagtctgag ttccctctgg
    11041 aataaccaat tcaacatcac cgtgaacagt tggaatatct actgtatcac caagagccgc
    11101 ttggacaaag ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc
    11161 gctagcttct acagaaacta ctacatacaa gtcaccataa ggtccaccgt taaagcctgc
    11221 ttcaccttga ccagcgaggc gaatttgttg acctgtttcc acaccagcag ggattttcac
    11281 atgtacgcta tgagcttgtt tctcatgacc tgttccatga caggttgtac atggatattt
    11341 gatttctttt cctcgaccgt gacagacatc acaggttact tggcgacgca tcataccaag
    11401 aggagtctgc gtatcgacgt taatgacacc agcgccatga cagcgtccac aagtgactgg
    11461 acttgtccct ggcttagcac cagagccatt acatgtacga cagccagctt cacgatgata
    11521 cttaacttcc ttctcagttc cgaagatagc ttcttcaaag gtcaaattga cacgatactg
    11581 gagatcatct ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa
    11641 acttgagaaa atatcctcaa aaccaccgaa accacctgcc ccattgaaac cgccgaaacc
    11701 accagctcca ccaaaaccac cattggcgcc tgcagcacca tactggtcat aggcagcacg
    11761 tttttggtcg tcactcaaag tctcataggc ttcttgaact tccttgtact tgtcctcagc
    11821 accaggctcc ttgttgatat ctgggtgata ttttttggaa agcttacgat aagccttttt
    11881 gatttcgtct gccgaagcgt ttttggatac ccccagacga tcataaaatt cagtattgtt
    11941 cataattcaa gatacaaagg gcgtcaaacg gacacagcca tctaggagtt ttgacgacgg
    12001 acgcttacgt cctagaaaaa actatctttt gggcacagtc cgtagcccgt gttcagttac
    12061 gaacaccctt attcctttct attgatgttc tgaatcgaac caccatttag gtcgggttca
    12121 attcctttct tttatattta gtaagaaatt ctgaaacccg tgttcagtta cgaacactct
    12181 ttgttccctt ctattgatga gacaaaaccc aaacaaatga gattccgtcc ctttttatcg
    12241 aaaaacagag gctgggttgc aacctctgca aataagtaaa ttaactttat tttgatttta
    12301 aatatcctat
//
LOCUS       DAVLFB010000059        12130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLFB010000059 DAVLFB010000000
VERSION     DAVLFB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.059"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            85..531
                     /gene="argR"
                     /locus_tag="VXD58_001419"
     CDS             85..531
                     /gene="argR"
                     /locus_tag="VXD58_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0676102.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            582..3116
                     /gene="mutS"
                     /locus_tag="VXD58_001420"
     CDS             582..3116
                     /gene="mutS"
                     /locus_tag="VXD58_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV0676103.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3263..4957
                     /gene="patA"
                     /locus_tag="VXD58_001421"
     CDS             3263..4957
                     /gene="patA"
                     /locus_tag="VXD58_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV0676104.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5287..5550)
                     /locus_tag="VXD58_001422"
                     /pseudo
     CDS             complement(5287..5550)
                     /locus_tag="VXD58_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5748..7514
                     /gene="patB"
                     /locus_tag="VXD58_001423"
     CDS             5748..7514
                     /gene="patB"
                     /locus_tag="VXD58_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV0676105.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7653..8342
                     /locus_tag="VXD58_001424"
     CDS             7653..8342
                     /locus_tag="VXD58_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV0676106.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            8346..8525
                     /locus_tag="VXD58_001425"
     CDS             8346..8525
                     /locus_tag="VXD58_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676107.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            8512..9861
                     /locus_tag="VXD58_001426"
     CDS             8512..9861
                     /locus_tag="VXD58_001426"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV0676108.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            9985..11445
                     /locus_tag="VXD58_001427"
     CDS             9985..11445
                     /locus_tag="VXD58_001427"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV0676109.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            11459..11890
                     /locus_tag="VXD58_001428"
     CDS             11459..11890
                     /locus_tag="VXD58_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0676110.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
     gene            12014..12085
                     /locus_tag="VXD58_001429"
     tRNA            12014..12085
                     /locus_tag="VXD58_001429"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12047..12049,aa:Glu,seq:ttc)
BASE COUNT         3610 a         2321 c         2759 g         3440 t
ORIGIN      
        1 gaagaaaaaa tttttgaaat ctcctgtttt tttggtataa tatggttata aatatagtta
       61 taaatatgca cgcaagagga ttttatgaga aaaagagatc gtcatcagtt aataaaaaaa
      121 atgattactg aggagaaatt aagtacacaa aaagaaattc aagatcggtt ggaggcgcac
      181 aatgtttgtg tgacgcagac aaccttgtct cgtgatttgc gcgaaatcgg cttgaccaag
      241 gtcaagaaaa atgatatggt gtattatgta ctagtaaatg agacagaaaa gattgatttg
      301 gtggaatttt tgtctcatca tttagaaggt gttgcaagag cagagtttac cttggtgctt
      361 cataccaaat tgggagaagc ctctgttttg gcaaatattg tagatgtaaa caaggatgaa
      421 tggattttag gaacagttgc tggtgccaat accttattgg ttatttgtcg agatcagcac
      481 gttgccaaac tcatggaaga tcgtttgcta gatttgatga aagataagta aggtcttggg
      541 agttgctctc aagacttatt tttgaaaagg agagacagaa aatggcgata gaaaagctat
      601 cacccggcat gcaacagtat gtggatatta aaaagcaata tccagatgct tttttgctct
      661 ttcggatggg tgatttttat gaattatttt atgaggatgc ggtcaatgct gcgcagattc
      721 tggaaatttc cttaacgagt cgcaacaaga atgccgacaa tccgatccct atggcgggtg
      781 ttccctatca ttctgcccaa cagtatatcg atgtcttgat tgagcagggt tataaggtgg
      841 ctatcgcaga gcagatggaa gatcctaaac aagcagttgg ggttgttaaa cgagaggttg
      901 ttcaggtcat tacgccaggg acagtggtcg atagcagtaa gccggacagt cagaataatt
      961 ttttggtttc catagaccgc gaaggcaatc aatttggcct agcttatatg gatttggtga
     1021 cgggtgactt ttatgtgaca ggtcttttgg atttcacgct ggtttgtggg gaaatccgta
     1081 acctcaaggc tcgagaagtg gtgttgggtt atgacttgtc tgaggaagaa gaacaaatcc
     1141 tcagccgcca gatgaatctg gtactctctt atgaaaaaga aagctttgaa gaccttcatt
     1201 tattggattt gcgattggca acggtggagc aaacggcatc tagtaagctg ctccagtatg
     1261 ttcatcggac tcagatgagg gaattgaacc acctcaaacc tgttatccgc tacgaaatta
     1321 aggatttctt gcagatggat tatgcgacca aggctagtct ggatttggtt gagaatgctc
     1381 gctcaggtaa gaaacaaggc agtcttttct ggcttttgga tgaaaccaaa acggctatgg
     1441 ggatgcgtct cttgcgttct tggattcatc gccccttgat tgataaggaa cgaatcgtcc
     1501 aacgtcaaga agtagtgcag gtctttctcg accatttctt tgagcgtagt gacttgacag
     1561 acagtctcaa gggtgtttat gacattgagc gcttggctag tcgtgtttct tttggtaaaa
     1621 ccaatccaaa ggatctcttg cagttggcga ctaccttgtc tagtgtgcca cggattcgtg
     1681 cgattttaga agggatggag caacctactc tagcctatct catcgcacaa ctggatgcaa
     1741 tccctgagtt ggagagtttg attagcgcag cgattgctcc tgaagctcct catgtgatta
     1801 cagatggggg aattatccgg actggatttg atgagacttt agacaagtat cgttgcgttc
     1861 tcagagaagg gactagctgg attgctgaga ttgaggctaa ggagcgagaa aactctggta
     1921 tcagcacgct caagattgac tacaataaaa aggatggcta ctattttcat gtgaccaatt
     1981 cgcaactggg aaatgtgcca gcccactttt tccgcaaggc gacgctgaaa aactcagaac
     2041 gctttggaac cgaagaatta gcccgtatcg agggagatat gcttgaggcg cgtgagaagt
     2101 cagccaacct cgaatacgaa atatttatgc gcattcgtga agaggtcggc aagtacatcc
     2161 agcgtttaca agctctagcc caaggaattg cgacggttga tgtcttacag agtctggcgg
     2221 ttgtggctga aacccagcat ttgattcgac ctgagtttgg tgacgattca caaattgata
     2281 tccggaaagg gcgccatgct gtcgttgaaa aggttatggg ggctcagacc tatattccaa
     2341 atacgattca gatggcagaa gataccagta ttcaactggt tacagggcca aacatgagtg
     2401 ggaagtctac ctatatgcgt cagttagcca tgacggcggt tatggcccag ctgggttcct
     2461 atgttcctgc tgaaagcgcc catttaccga tttttgatgc gatttttacc cgtatcggag
     2521 cagcagatga cttggtttcg ggtcagtcaa cctttatggt ggagatgatg gaggccaata
     2581 atgccatttc gcatgcgacc aagaactctc tcattctctt tgatgaattg ggacgtggaa
     2641 ctgcaactta tgacgggatg gctcttgctc agtccatcat cgaatatatc catgagcaca
     2701 tcggagctaa gaccctcttt gcgacccact accatgagtt gactagtctg gagtctagtt
     2761 tacaacactt ggtcaatgtc cacgtggcaa ctttggagca ggatgggcag gtcaccttcc
     2821 ttcacaagat tgaaccggga ccagctgata aatcctacgg tatccatgtt gccaagattg
     2881 ctggcttgcc agcagacctt ttagcaaggg cggataagat tttgactcag ctagagaatc
     2941 aaggaacaga gagtcctcct cccatgagac aaactagtgc tgtcactgaa cagatttcac
     3001 tctttgatag ggcagaagag catcctatcc tagcagaatt agctaaactg gatgtgtata
     3061 atatgacacc tatgcaggtt atgaatgtct tagtagagtt aaaacagaaa ctataaaacc
     3121 aagactcact agttaatcta gctgtatcaa ggagacttct ttgacaattc tccacttttt
     3181 tgctagaata acatcacaca aacagaatga aaaggagctg acgcattgtc gctccctttt
     3241 gtctattttt taaggagaaa gtatgctgat tcagaaaata aaaacctaca agtggcaggc
     3301 cctggcttcg ctcctgatga caggcttgat ggttgctagt tcacttctgc aaccgcgtta
     3361 tctgcaggaa gtcttaggcg ccctccttgc tgggaaatat gaagctattt atagtatcgg
     3421 ggcttggttg attggtgtgg ccgtagtcgg tctagttgct ggtggactca atgttgtcct
     3481 cgcagcctat attgcccaag gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat
     3541 tcaaaccttt tcttatgctg atattgaaca atttaatgcg ggaaatctag tcgttcgaat
     3601 gacaaatgat atcaaccaga ttcagaacgt tgtcatgatg accttccaga ttcttttcag
     3661 acttcccctc ttgttcatcg gttcgtttat cctagcggtt caaaccttac cttctctgtg
     3721 gtgggtgatt gttctcatgg tagtcttgat ttttggtttg actgctgtca tgatgggaat
     3781 gatggggcct cgttttgcca agtttcaaac ccttcttgag cgcatcaatg ccattgccaa
     3841 ggaaaattta cgtggcgttc gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc
     3901 taagtttaca gaggtctcag acgagcttct tggtcaaaac ctttacattg gttatgcctt
     3961 ttcagtagtg gaacccttta tgatgttggt tggttacggg gcggtcttcc tctctatttg
     4021 gctggtcgcg ggaatggttc agtcggatcc gtctgttgtt ggttccatcg cttcttttgt
     4081 taattaccta agccagatta tctttaccat tgttatggtt ggatttttgg gaaattctgt
     4141 cagccgtgcc atgatttcca tgcgtcgtat tcgagaaatt cttgacgcag agccagctat
     4201 gaccttcaag gatatcccag atgaagagtt ggttggaagc cttagctttg aaaatgtgac
     4261 ctttacctat ccaatggaca aggaaccgat gctgaaagat gtgagcttta ctattgaacc
     4321 tggtcaaatg gttggtgtag ttggagcgac tggtgcagga aagtcaacct tggctcaatt
     4381 gattccacgt ctctttgatc cacaggaagg ggctatcaaa atcggaggta aggatattcg
     4441 agaagtgagt gaaggaaccc tgcgtaaaac agtttccatc gttctccaac gtgccattct
     4501 ttttagtgga acgattgcag ataacttgag acaggggaag ggaaatgcta ctctatttga
     4561 aatggagcgc gcagccaata ttgcccaggc tagtgaattc attcatcgta tggagaaaac
     4621 ctttgaaagt ccagttgaag aacggggaac caatttctct ggtggacaaa aacaaaggat
     4681 gtcgattgcg cgtgggattg tcagcaatcc acgtattctg atttttgatg attcgacctc
     4741 agccttggat gccaaatcag agcgcttggt gcaagaagct ttgaataagg acttgaaggg
     4801 gacgacaacc attattattg ctcaaaaaat tagctcggtt gtccatgcag acaagatctt
     4861 ggttctaaat caaggacgat tgattggtca aggcacgcat gcagacttgg ttgccaacaa
     4921 tgccgtttac cgtgaaatct atgaaacaca gaaatgaaag acaaactata agaaaagtca
     4981 atagttttat ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca
     5041 cggcaaaaag ctattgaaaa agtccatttt ttcaaaggta atcctgtgtt aatttcagaa
     5101 attacatcac tttttgttcg tcaaatggca gctctttttt aggatataaa acagggttcg
     5161 gataagtttt tttgcaaggt ggatgatggc tacattgtaa tgttttcctt gttctaactt
     5221 agtcttaaga taggccttaa aagcaggtga aaagcgaggg catgctttgg cagcttgtat
     5281 gagtacctac cgcagatgag gggaaccccg tttgaccatt cttcctgcta aatcaatctg
     5341 acctgactga taaatagaag aatccagtcc agcgaaagct tgtaattgag caggattatc
     5401 aaaggcatga atatttcgaa tctcggctaa aatgaccgcc cctaaacgac ccccaatccc
     5461 agtaaccgtc gtgatgaccg agttgaactc agccatcaag tcattgacac attttttcgc
     5521 cttgtcaatg agcctcttgt aatgtttgat gttttcatta caagagataa aacgtttatg
     5581 cgttatcaaa ctcattacca attgaaacaa aaagctgtgg ttagatcctt tcggaaattg
     5641 tcaagcgatt ggaggaaatg aactaatcca cagcggctta ttccaagtat accacttggg
     5701 ctttggcagt agctaactgc gctaaatata atataaggag gagtaaaatg aagacagttc
     5761 aatttttttg gcattatttt aaggtctaca agttctcatt tgtagttgtc atcctgatga
     5821 ttgttctggc gacttttgcc caagccctct ttccagtctt ttctggacaa gcggtgacgc
     5881 agctagccaa tttagttcaa gcttatcaaa atggcaatcc agaacttgta tggcaaagcc
     5941 tatcaggaat catggtcaat cttggcctgc tggttttggt tctatttatc tctagtgtaa
     6001 tatacatgtg tctcatgacg cgcgtgattg cagaatcgac caacgagatg cgcaaaggcc
     6061 tctttggtaa gcttgctcag ttgacggttt ctttctttga ccgtcgacaa gatggcgata
     6121 tcctgtctca ttttaccagt gatttggata atatcctcca agcctttaac gaaagcttga
     6181 ttcaggtcat gagcaatatt gttttataca ttggtctgat tcttgtcatg ttttcgagaa
     6241 atgtgacgct ggctctcatc accattgcca gcaccccatt ggctttcctt atgctgattt
     6301 tcatcgtgaa aatggcacgt aaatacacca acctccagca gaaagaggta gggaagctca
     6361 acgcctatat ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa ggaattcaag
     6421 aggatatgat ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca acctttaaag
     6481 gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg gatgagcctg attaatacag
     6541 ccatcgtcat ctttgctggt tcggctgtac ttttgaatga taagtctatt gaaacaagta
     6601 cagccctagg tttgattgtt atgtttgcac aattttcaca gcagtactac cagcctatta
     6661 tccaagttgc agcgagttgg ggaagccttc agttggcctt tactggagct gaacgaattc
     6721 aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc ttcactaagt
     6781 tgcaagaaag tgttgaaatc agtcatatcg atttttcata cttgcctgat aaacctattt
     6841 tgaaagatgt cagcatttct gcccctaaag gccagatgac agcagttgtt gggcctacag
     6901 gttcaggaaa aacgactatt atgaacctca tcaatcgctt ttatgatgtt gatgctggtg
     6961 gtatttattt tgatggtaaa gacattcgtg gctatgactt agatagtctt agaagcaagg
     7021 tgggaattgt attgcaagat tcggtcttgt ttagcggaac gattcgagac aatatccgat
     7081 ttggtgtgcc agatgctaat caggaaatgg ttgaggtagc agcaaaagca acccacattc
     7141 acgactatat cgaaagtttg cctgataagt acgatactct tattgatgat gaccagagca
     7201 tcttttcaac agggcagaag caattgattt caatcgctcg aaccctgatg acagatccag
     7261 aagttctcat tctcgatgaa gcaacttcaa acgtagatac ggtgacggaa agcaagattc
     7321 agcatgccat ggaggtggtt gtagcaggta gaactagttt cgtcattgcc caccgcttga
     7381 aaaccattct caatgcagat cagattattg tccttaaaga tggagaagtc attgaacgtg
     7441 gtaaccacca tgaacttttg aagctaggtg gcttttattc agaactctat cacaatcaat
     7501 ttgttttcga ataagaaaga agttgtccta tgtgggcagc tttttcttgt ccataaaaaa
     7561 tgtttatcac agccttaaaa aaaacatatt agacgaaagt cattttgagt gatatgatag
     7621 gactatcgtt agtattcgaa aggagaggca tcatggctag aacggttgta ggagttgctg
     7681 caaatctatg tcccgtagac gcagaaggca aaatcattca ttcatctgta tcttgtagat
     7741 tcgcagagat cattcgtcaa gtcggtggtc tccctttagt cattcctgtt ggtgatgagt
     7801 cagttgtacg tgattatgtg gaaatgattg acaaactcat tttgacagga ggccaaaatg
     7861 ttcatcctca gttttatgga gagaaaaaga ccgtcgaaag cgatgattac aatctggtcc
     7921 gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg tcagaataaa ccaattatgg
     7981 caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc aatcaagaaa
     8041 tcgaaggtca ctggcaagga ctacctttcg ggacatctca ctctattgaa acagtagaag
     8101 gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt caactcagtc catcgtcaaa
     8161 gcattaaaga tttggcacct aatttccgtg taactgctat tgattcaaga gaccaaacta
     8221 ttgaagcgat tgagtctatc gacgagcacc gcattatcgg tttacagtgg catccagagt
     8281 ttctggttaa tgaagaagat ggcaatttag aattatttga gtatttattg aatgaactgt
     8341 aacgattgga acatctggtc gttctttctt ttattttttc aaaattttta gaatgtggca
     8401 ttttacgcaa acgtttgaat tctgataaaa attggagaaa ttcgacaaaa aaacttgaaa
     8461 aaaacgaagg taagcgttat gatagaaaag aagaaatatt ggaggaagaa catgtcacat
     8521 attaaatttg attattcaaa agttttagac aaatttgttg caccacatga agtggaatac
     8581 atgcaatcac aagtaacagc agcagatgaa ttgatccgta aaggaactgg tgctggtagc
     8641 gacttcttgg gatggttgga ccttcctgaa aaatacgacc gcgaagaatt tgaccgcatc
     8701 ttgaaagctg ctgagcaaat caagtcagac agcgatgttt tggttgtgat cggtattggt
     8761 ggatcttacc ttggtgcaaa agcagcaatc gacttcttga atcaccattt tgctaacttg
     8821 caaacaaaag aagaacgcaa agctccacaa atcctttacg caggaaactc aatctcatct
     8881 acttaccttg ctgacttggt agagtatgtt gcagacaaag acttctcagt aaacgtaatt
     8941 tctaaatcag gtacaacaac tgaaccagcg attgctttcc gtgtctttaa agaactcttg
     9001 gttaagaaat acggtcaaga agaagctaac aaacgtatct atgcaacaac tgaccgccaa
     9061 aagggtgctg ttaaggttga agcagacgct aacggttggg gaacatttgt tgttccagat
     9121 gatatcggtg gacgcttctc agtattgaca gccgttggtt tgcttccaat cgcagcatca
     9181 ggagctgaca tcaaagctct tatggaaggt gcgaatgcag ctcgcaaaga ctacacttca
     9241 gacaaaatct ctgaaaacga agcttaccaa tacgcagtcg ttcgtaacat cctttatcgt
     9301 aaaggctatg caactgagat cttggtaaac tatgagccat cacttcaata cttctcagaa
     9361 tggtggaaac aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat ctacccaact
     9421 tcagccaact tctcaactga cttgcactca cttggtcaat ttatccaaga aggaactcgt
     9481 atcatgtttg aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct tattcctact
     9541 ttggaagaag accttgacgg acttggttac cttcaaggaa aagacgttga ctttgtaaac
     9601 aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc aaacatgtat
     9661 gtgactcttc cagagcaaga cgctttcact cttggttaca ctatctactt cttcgaattg
     9721 gcaattgccc tttcaggtta cttgaatgct atcaacccat ttgaccaacc aggtgttgaa
     9781 gcttataaac gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga attgagcaaa
     9841 gaacttaacg cacgtctata atagaagaaa agagtggttt gcccactctt tttactctct
     9901 ttatccatag aaattggact cagccaagac ttgtgatata atatagaaag caaaaaggca
     9961 gacgcctaga taataggaga aactatgtca aaagatatcc gcgtacgtta cgcaccaagt
    10021 ccaacaggac tactacacat cggaaatgcc cgtacagcat tgtttaacta cttgtatgcg
    10081 cgccatcatg gtggaacatt tctcatccgt atcgaagata ctgaccgtaa acgccatgtc
    10141 gaggatggtg aacgttcaca acttgaaaac cttcgctggt taggcatgga ttgggatgaa
    10201 agtccagaat cacatgagaa ttatcgccag tctgagcgtt tggacttgta tcaaaaatac
    10261 attgaccaac tattagctga aggaaaagcc tataaatctt acgttacaga agaagagttg
    10321 gcagctgaac gtgaacgtca agaagtagct ggcgaaacac cacgctacat caatgaatac
    10381 cttggtatga gtgaagaaga aaaagcggct tatatcgcag aacgtgaagc agctggtatc
    10441 ataccaacag ttcgtttggc tgtcaatgag tcaggtatct acaagtggca tgatatggtc
    10501 aaaggtgata tcgaatttga aggtggcaat atcggcggtg actgggttat ccaaaagaaa
    10561 gacggttacc caacttacaa ctttgccgtt gttatcgatg accacgatat gcaaatctct
    10621 catgttatcc gtggagatga ccatattgct aatacaccaa aacagcttat ggtctatgaa
    10681 gctcttggtt gggaagctcc agagtttggt cacatgacct tgattatcaa ctctgagact
    10741 ggtaagaagt tatctaaacg tgatactaat acacttcaat ttatcgaaga ttaccgaaag
    10801 aaaggttacc ttccagaagc agtctttaac tttattgctc ttcttggttg gaacccaggt
    10861 ggtgaagatg agattttctc tcgtgaagaa ctcattaaac tttttgatga aaaccgcctc
    10921 agcaagtcac cagcagcctt tgatcagaaa aaattagact ggatgagcaa tgattatatc
    10981 aagaatgcag accttgaaac catctttgaa atggcaaaac cattcttaga ggaagcaggc
    11041 cgtttgactg acaaggctga aaaattagtt gagctctata aaccacaaat gaaatcagta
    11101 gatgagatta tcccattgac agatcttttc ttctcagatt tcccagaatt gacagaagca
    11161 gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt caaagcaaaa
    11221 cttgaagcga tgacagatga tgaatttgtg acagaaaata tcttcccaca aattaaagca
    11281 gtccaaaaag aaacaggtat taaagggaaa aatcttttca tgcctattcg tatcgcagtt
    11341 tcaggcgaaa tgcatgggcc agaattacca gatacaattt tcttgcttgg acgtgaaaaa
    11401 tcaattcagc atatcgaaaa cattctaaaa gaaatctcta aataaagagg atacaataat
    11461 ggacatctgg gaaaagatgt acgaagaagc acagaaacta tacaatccac atgaagtatc
    11521 agactttgtt tatgccaatc atgttgtagc tgcagtagaa gcaggagatg gacaaatatt
    11581 tacaggattc tgtatggagg gaacctgtgg tgttttccat ctctgcgcag aacgggcgac
    11641 cctcttcaat atgtaccaat tttcaggaca aactaaggtt aagaaagtat tagcctttcg
    11701 agacaaacca ccttatggtg gaagttcagc catgccttgc ggtgcttgta gagaattcct
    11761 tttagagctt aatgctgaaa ataaagatgc agaattcatg atggactata atataagaaa
    11821 aacagttaaa gtcgcagaac taatccctta ttggtgggga gaagaacgtg cttctaagtt
    11881 taatgagtga tagaagagtc agtataattc tggctctttt tactataagt tgaaaaaata
    11941 aaaaaaatta gaaaaagtag ttgacaaaat ttgaaaagac tgtataatag taagagttga
    12001 aaataacaac tcaggtccgt tggtcaaggg gttaagacac cgccttttca cggcggtaac
    12061 acgggttcga atcccgtacg gactatggta tgttgcgtca ggaccacttg atgaaaaaaa
    12121 gtttaaaaaa
//
LOCUS       DAVLFB010000060        12006 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLFB010000060 DAVLFB010000000
VERSION     DAVLFB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.060"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            79..546
                     /locus_tag="VXD58_001430"
     CDS             79..546
                     /locus_tag="VXD58_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV0676111.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            579..672
                     /gene="ffs"
                     /locus_tag="VXD58_001431"
     ncRNA           579..672
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VXD58_001431"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(755..1896)
                     /locus_tag="VXD58_001432"
                     /pseudo
     CDS             complement(755..1896)
                     /locus_tag="VXD58_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1951..3021)
                     /locus_tag="VXD58_001433"
     CDS             complement(1951..3021)
                     /locus_tag="VXD58_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV0676112.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(3038..3418)
                     /locus_tag="VXD58_001434"
     CDS             complement(3038..3418)
                     /locus_tag="VXD58_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV0676113.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(3431..3694)
                     /locus_tag="VXD58_001435"
     CDS             complement(3431..3694)
                     /locus_tag="VXD58_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV0676114.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(3694..3927)
                     /locus_tag="VXD58_001436"
     CDS             complement(3694..3927)
                     /locus_tag="VXD58_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676115.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(3927..4295)
                     /locus_tag="VXD58_001437"
     CDS             complement(3927..4295)
                     /locus_tag="VXD58_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676116.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            4867..5058
                     /locus_tag="VXD58_001438"
     CDS             4867..5058
                     /locus_tag="VXD58_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0676117.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            5081..5284
                     /locus_tag="VXD58_001439"
     CDS             5081..5284
                     /locus_tag="VXD58_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676118.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(5439..5606)
                     /locus_tag="VXD58_001440"
     CDS             complement(5439..5606)
                     /locus_tag="VXD58_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV0676119.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            5626..5991
                     /locus_tag="VXD58_001441"
                     /pseudo
     CDS             5626..5991
                     /locus_tag="VXD58_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(6211..6390)
                     /locus_tag="VXD58_001442"
     CDS             complement(6211..6390)
                     /locus_tag="VXD58_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676120.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6532..6681)
                     /locus_tag="VXD58_001443"
     CDS             complement(6532..6681)
                     /locus_tag="VXD58_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676121.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            6986..7429
                     /locus_tag="VXD58_001444"
     CDS             6986..7429
                     /locus_tag="VXD58_001444"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV0676122.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            7431..7946
                     /locus_tag="VXD58_001445"
     CDS             7431..7946
                     /locus_tag="VXD58_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0676123.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            7960..9321
                     /gene="radA"
                     /locus_tag="VXD58_001446"
     CDS             7960..9321
                     /gene="radA"
                     /locus_tag="VXD58_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV0676124.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            9394..9891
                     /locus_tag="VXD58_001447"
     CDS             9394..9891
                     /locus_tag="VXD58_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV0676125.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            9916..10699
                     /locus_tag="VXD58_001448"
                     /pseudo
     CDS             9916..10699
                     /locus_tag="VXD58_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            10844..11812
                     /locus_tag="VXD58_001449"
     CDS             10844..11812
                     /locus_tag="VXD58_001449"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0676126.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
BASE COUNT         3332 a         2050 c         2667 g         3957 t
ORIGIN      
        1 caccgcattc ggtggcttct tttgtgttgg gattcatgat ataataataa aatcgataag
       61 taggaaaaga gaaaagagat gtattatacg cttgaagaaa aagaagtctt tatgagggag
      121 gctttgagag aggctgagat tgctcttgaa cacgatgaaa ttccaattgg ttgtgtgatt
      181 gtcaaagatg gggaaatcat tggtcgtggg cataatgcgc gtgaggaatt acagcgagcg
      241 gttatgcatg cggaaattat ggctatagag gatgcgaact tgagtgagga gagctggcgc
      301 ttgctggatt gcacactttt tgtgaccatt gaaccttgtg tcatgtgtag tggggcgatt
      361 gggcttgccc gcattccaaa tgtggtctat ggggctaaaa accagaaatt tggcgctgct
      421 gggagtttgt acgatatctt gacagatgag cgtctcaatc atcgtgtgga ggttgaaata
      481 ggaattttgg aagatgaatg cgcagctatc atgcaggatt tttttagaaa tagacggaaa
      541 aaataatttt gcttttaaaa tgaataggaa tgtgatataa taaatagtgg agcaacagtt
      601 ctgcgtgaag cgggtcaggg gaggaatcca gcagccctaa gcgatttgaa ttgtgtgctc
      661 ttttttcgtg cactttaaaa accctttaaa atcaacactt taaggggttt ttgtttgtct
      721 tgtataagaa aaaggggcag acgaggggca caatttaaaa ttttatcttg tctaacttgc
      781 tagatatgtc tgataccatt ttttgagtaa cgtgagaata aatctctagt gtggtctttg
      841 agtcactatg ccctactctg tccatgatgg cagtcaaggg aatgcccagc tcagcaagta
      901 gggatatgtg agaatgtcta aatgtatgtg tagttatgtt tttttctatg ccgatttttt
      961 gaccatgtct tttcaatgca caaataaccc tggcatttgt tattggttct cctagagtat
     1021 tgatgaaaat aaaatctgta tcaaatccat ttgtcgcatt ctctattatt tgctctttga
     1081 taatatctaa cactttttga ggtgctgtta taaccctatc ggacttgatt gtctttggtg
     1141 tagttctctc tttttgtctg aaatcgtatg tatgcttgat gtgaatagtc tttttagaaa
     1201 aatctatatc ttccttgtag tttaaggctg ccagctctcc atacctcatg ccagtaagaa
     1261 aaagaacttt agctattcgg atatactttg taattcgata atcacatagg gcctcgtctt
     1321 ttaaattttg gatgaataac ttaaactctt tttggtctaa gtattttgta tttttctttc
     1381 tgagttcgtc ggatgtaatt acttttctag gcgtttcaac aaatagcatt tcatttgtat
     1441 caatataatt cattctgata gcaaatttca ttatctgatt gagcttgaat ttgattttag
     1501 aaacatagtt atgagatctc ccatcttgta atagttgatc tattactttt tgtaataaac
     1561 gtctatcaaa tttctaacta ggtagtcgcc ctctatctgc tttaaaatct ctttttttac
     1621 attctttgaa gcgtagacgg ttgagttttt tacgccgtgt ttccaattct cctcaaattc
     1681 ttcatatagt ttttcaaaag ttatattaga aacagaatgt tgtttttctc ctaacttttg
     1741 ttttatcttt tcctgaagta aaatcgcagc ttgatttctt gcttggggag tttttttctc
     1801 catcgttact gagacttttt ttaatttctc agtatatgga tctttgtatc tctcaaaaaa
     1861 tttatatttg ccgtttggca attcttccat ccacattgat tttacctcac ttttttgata
     1921 aaatgggtat agtaaagagg gcttttttta ttatgagaat ttcttgatta cttctaaaat
     1981 ttcattggca taatttgcga cttcgagagg ggtagacacc gggaagatgc ctttatcacg
     2041 gatttcgata gtgctttttt ttgaatttga acgataacgt aatacccatt ttttgatgtt
     2101 atcgtcaacc aaaacattga aatagctacg gttatctctg taaaatacac gttctggaga
     2161 aactacatcc ctagcaagca ttttaacaac agtataaact tctaattcag caggagttgt
     2221 gataatttcg tcagttactt cgacaatctc ttcagcttca gcttcaactt ttggaatatc
     2281 ggttgttact ttggtttcaa cgcttgtatt aagtgcagca cttaattttt cattaactcg
     2341 ctctgtgatg aattgattaa accctttcac aataatggga gaaaatgtcg ttaagatatt
     2401 ttgagtaaca cgcccttcat agatttcaga agttagatat ctgaggaagc tatctgacgg
     2461 tgtagtgata ttttcagtaa ggaacgcttt aagattgttg aggtatttca attcagaagc
     2521 tgatgaaaca atattatcaa tatcaaaatt ctctttgtgg aatttgatga tttcagtaaa
     2581 ttgattttct ttgatatcag tcacatcgat tgttaagaat ggagttgtgt ccattttatt
     2641 tggctcatct aaatcagtaa agaatttata ttctctacca tttgtcaaga taccgaattt
     2701 tgatttagta gttacgaaat atctgaatag ttgagagtcg tgcttagtaa ggttttctgt
     2761 aattgattta cattcaatta ggatttgagg ctcgccatcc aaaatgatcg catagtcgac
     2821 tttttcgcct tttttaatgc ccacgtcggc agtaaattcc gggacaaatt caagtggatt
     2881 gaagatatca tatccaagcg cttggaagaa cggcataatg aaggcattct ttgtttgttc
     2941 ttcattcgta atgctttggc tgaggtctgc tacacgtttg ccgacttgtt ttaaatcggc
     3001 ttttacttta tcaatttcca tattgatact ccttttttta atttactaat gctaaatact
     3061 cttctttaac catgatttca tttgtcatgg tttttagatt gtagtaagac atgaatttga
     3121 ggtaatcaaa ctctgtgggg tcgtctaagt tttctagagc gtcttttacg agatgatgga
     3181 tcatattcct atcagcttcg ttttcacagc gtagacgggc gttctggtgc tctgagcgtg
     3241 tgtggtcttt gtgtccaagt tcgtgtaggg cgacttggat ttgttgttca ggagtcaagt
     3301 tgtggtcaat agcgagcacg tttgtgtctg gattgtagaa accgctattg tgccagtttg
     3361 aaccgtcgaa gagacaaagt tctacttgat attcttcgca gagtttagcg agtgtcatag
     3421 ttctccttcg ttatttatag acatctctgc ggtgggcgat ttccacggct aggacgacta
     3481 gtttatcgtc ttggatatca cagatgatgc ggtaattctc tactctgtat cgccagtaac
     3541 ctgcaaggtt ggcttttagt gcttttccgt gttgtcgtgg attggtcgtg ttttcaatgt
     3601 ttttagcaag ccaggatagg atttgttttc tggttggggt atctagtttt ttaagttgct
     3661 tgagagcttt tttatcaata tctagccgat acattaagca atatcctctc gagttagtcc
     3721 tagttcatcc aagacctcat ccatggtata agtagctggg tcggctaaat actccatata
     3781 ggcttggtcg gctgctcgtg cgtcttcgat atcttccatg agtgccatga agtcgtcaaa
     3841 atccatggtc gttgtgtcga taccgtgttt gtttaggtag tccgtaatgt aggaattttt
     3901 ttctgtgaag ttgatagtga tagtcattag cgttctcctt tgcttttgaa gtgggcggat
     3961 aggacggatg taatgaagtc gatatcatct tcatttagtg gtttcccgtc aaataacatg
     4021 gtgttggctg ctgctttgcg taagtctatg atttgtccgt ttacttgggc aaattcatca
     4081 ctcccagcga tagcagggtt atctgttcgt ccgagcaggt agtcggtgga tacgttgaaa
     4141 taattagcaa tttctgcgat acgttcagcg tttggcgtag aattttttat cttatagagt
     4201 gtatttctac cataacctaa gtcttcttct acttgtccaa gagcttttcc acgctttttt
     4261 gccaattctt ttattttttc aaatgtctca aacattgtta aatcaacctt tctaagacat
     4321 cacaaaaaaa atttaacaaa tttggtgtaa aaaggttgac taattatccc aaaaggtgta
     4381 aaatgttttt tgtaagtgat aaacaactaa aaaaacaact aagaaataaa ttataaaaat
     4441 gttttggcga acggtattta tagatttatt ggtgttttta ttatgcttta attttagccg
     4501 atttggtgta agttgtcaag tataatgcag aaaaatagtt acatttttag ttgtttctta
     4561 tttacaaata agtaaagagg aggaacgtgc tgatggtaag tattcttaaa aatttagaac
     4621 aagaaaaaga ccaccttgaa aaagtcatta aggtagtcag cgctggtggt aaatttctga
     4681 gattgccata tcaaaaaagt cacgctcgat tagtgagaat ctgaaattga tttctcaaaa
     4741 tctttaccat catcgtagat gtcgcgtttg cttgctttga gtaaatattc tttaaaagtc
     4801 ataaggttaa ctcctttctg cttatattat agcagaaaaa gaagaaagaa aaagaaagga
     4861 gaaaatatgc cagatatcgc aaacggtcgc gaaagagtta ttgctttctt gaaagagaaa
     4921 ggcattaaaa aagcaactct agcggttgct tacggcttta aacgacagga agtgacaaac
     4981 attctaagtg gaacgactaa aggtccacga gcgaacagtt tcattcttca ggttattgaa
     5041 gattatggga ttgagtagga aagatttgag gagtaggaaa atgagaccaa aacgatatcc
     5101 gtatagtgga caaaaagagt ccacctttgt aaaggcagac cctgagttag ttgaaaaact
     5161 tttaagaaac actagttttc ttgagtgttt acaaaaaaag cctatcaatt ttcagataga
     5221 ctcagaagaa tttaagcgtc ttagctatga agccattcat gatacttctc aagtaactca
     5281 ataggaggaa ggaataaaat gattcaccat tatataactc actatgccag caatgggaaa
     5341 gattacgccg aagcatggat tcaaattgat tttttgggaa tgtgcttttg tgtatggaaa
     5401 aagcgtacaa ccattgaacg attgtacgca aacgaagatt agactttttt ccaaccgttg
     5461 cctttagcag atgtcggagg gagccgatca ccttttccga tagttgcggt atgaccatta
     5521 gtaacttttc cgccacgagg tcctacctct acatagcgac caggtttctg attatctgtt
     5581 ccaggtttta ttggagtatt tgccatacta tctcctcctt tctataagtt tgagaaaatc
     5641 aacggtatct ggtattattt cgatggctca ggctatatgc tttcagaccg ctggaagaag
     5701 cacacagacg gtaattggta ctactttgac caatcaggcg aaatggccac aggctggaag
     5761 aaaatcgctg acaagtggta ctattttgat gtagaaggtg ccatgaagac aggctgggtc
     5821 aagtacaagg acacttggta ctacttagac gctaaagaag gcgccatggt atcaaatgcc
     5881 tttatccagt cagcggacgg aacaggctgg tactacctca aaccagacgg aacactggca
     5941 gacaagccag agttcacagt agagccagat ggcttgatta cagttaaata aatagaaagg
     6001 aaactttcta aattgttctt tcaccgcagg ctcaggcttg cggtttttta tttgctctga
     6061 ttcttcaaaa aagcgttttc ttgaagaata gggaggagga atggcagggt attattgcca
     6121 aaaacgccat tttgttaata atagctcctt tttatttttt gattattgtc aaaaacggtg
     6181 ttttgtcaaa aataaaaaca gtgaccgaaa tcactgctta tcagttgtag caaattcata
     6241 aagtttttct gctgtgagaa gtgccatttt gtccatgctt gtttttcctt ttctgaggtc
     6301 agaaacagta gtccatggaa ctccagcacc ttgggaaata gcagatgtag acatcgaact
     6361 gtctaacaat tcttgaataa cttctctcat cttatttgtc ctttttattt tttagataaa
     6421 tatatacatt gaccgcaatt ataaaaatag ctattgcact aaccattgct tttcctcttt
     6481 tcatttgata aaatagaggt gtgaggggct ttcgccccaa cctcttagcg ttcacctttt
     6541 tctttgacgg gatttcggtt tacgcttttt gttttgcctt gcgaccgtta ttgcagtcac
     6601 cagacttgct atagcagtta ccgtttcagg gatattatct atcgcctttt ccagtaacct
     6661 aagccaatct tctttgttca acttcctcac ctcctttcct tatcttgatt atattatatc
     6721 acggtaaacc gtgaaagtca agcgatttga tgaagttttt ttgaaaaaca aaggggcaaa
     6781 aaaggggcaa aagtgtcgta aacctctgta aaacgatgta aaaaatcaac tttgccctcg
     6841 ctttaaagct ctaaatttca acgtattgtg aaacagtgta aattatcgta tcgcctataa
     6901 ttgttgtgtg ctcttttttt cgtgcttttt ccgaataaat aagatagaat aatctagaat
     6961 aaatgataat agaaaagaga aaattatgaa aattcgtggt tttgaattgg tttcgagttt
     7021 tacagatgaa aatttattgc ccaagcgtga gacagcgcat gcggctggtt acgacttaaa
     7081 ggttgctgtg cgtacagttg ttgcgccagg agagattgtc ttggttccga caggggttaa
     7141 ggcttatatg cagccgactg aggttctcta cctctatgat cgttcttcaa atcctcgtaa
     7201 gaagggcttg gttttaatta actcagttgg ggtcattgat ggggattatt atggaaatcc
     7261 tggaaatgaa gggcatattt ttgcgcagat gaagaatatc acagatcaag aggttgttct
     7321 tgaagttggg gaacgtattg tccaggctgt ttttgctact ttcttaattg cagatggaga
     7381 tgcagctgat ggcgttcgaa ctggtggatt tggatcgaca gggcactaga atgaagatta
     7441 tctttgtacg tcatggggag ccagattacc gtgagttaga ggagcgttct tatataggat
     7501 ttgggataga tttggcaccc ttgtctgaga tgggacggca gcaagtccag aaattgagca
     7561 aaaatccttt actctcgtca gctgaaataa tcgtatcttc tgcagtcaca agagctttag
     7621 aaacggcttc gtatgtggtc tgtgctacgg gtcttccttt aagagtagag cctttattac
     7681 atgaatggca ggtctataaa acaggaatag aaaactttga aacagctaga agactgtttt
     7741 tagaaaacaa gggggagttg cttcctaata gtcctattca atatgagaca gctacggaaa
     7801 tgaagtctcg gtttctagaa tgtatgtcta agtatcgaga acatcagact gtggtagttg
     7861 ttgctcatcg aatgctcatg cgccagtttg tgccaaatga gaagattgat ttttgccaag
     7921 tgattgagtg tgagttagag atatagaaag aggtttgtca tcgcaaagaa aaaagcgaca
     7981 tttgtatgtc aaaattgtgg gtataattcc cctaaatatc tgggacgttg ccccaactgt
     8041 gggtcttggt cttcttttgt ggaagaggtt gaggttgccg aggtcaagaa tgcgcgtgtg
     8101 tccttgacag gtgagaaaac caagcccatg aaactagctg aggtgacttc catcaatgtc
     8161 aatcgaacca agacggagat ggaggaattc aaccgtgtgc ttggaggcgg agtggtacca
     8221 ggaagtctcg tcctcatcgg tggggatcct gggattggga aatcaactct tctcctacaa
     8281 gtctcaaccc agttgtccca agtggggaca gttctctatg tcagtgggga ggagtctgcc
     8341 cagcagatta aactacgtgc agagcgctta ggtgatattg atagtgagtt ttatctctat
     8401 gcagagacca atatgcagag tgttcgtgca gaagtggagc gtatccagcc agactttctc
     8461 attattgatt ccatccagac catcatgtct cctgagattt caggggtgca ggggtctgtt
     8521 tctcaggtac gtgaagtgac cgctgaactc atgcagttgg ccaagaccaa taacattgcc
     8581 atctttatcg taggtcatgt gaccaaagaa ggaaccttgg ctgggcctcg tatgttggag
     8641 catatggtgg atacggtgct ttactttgaa ggggagcgtc accacacctt tcgtattttg
     8701 agagcggtca aaaatcgttt tggttccact aatgagattg ggatttttga gatgcagtcg
     8761 ggcggcttgg ttgaggtact caatccgagt caagttttcc tagaagagcg tttggatggg
     8821 gcgactggtt cctccatcgt tgtaaccatg gaagggacgc gtccgatttt ggcggaggtt
     8881 caggctttgg taacaccgac catgtttgga aatgccaagc gtactacgac aggacttgat
     8941 tttaaccgtg ctagcttgat tatggctgtt ttggaaaaac gggcagggct tctcttgcaa
     9001 aatcaggatg cctatctcaa atctgctggt ggtgttaaat tggatgaacc tgccattgac
     9061 ttggctgttg cagttgctat tgcttcgagc tacaaagaca agccaactaa tcctcaggaa
     9121 tgttttgtcg gagaactggg cttgacagga gagattcggc gcgtgaatcg tattgagcaa
     9181 cgcatcaacg aagctgctaa actgggcttt actaagattt atgtacctca gaattccttg
     9241 acaggaatca ctctgcctaa ggaaattcag gtcattggcg tgacaacgat tcaggaagtc
     9301 ttgaaaaagg tctttgcata atccgtgaca aattctctta aaaatgataa gataggagaa
     9361 atatttgact atcaaatttt caaggaggga atcgtgtcgt attttgaaca gtttatgcaa
     9421 gccaatcagg cttatgttgc cctacatggg cagttaaatc tgccacttaa acctaaaacc
     9481 agagtagcca ttgtgacctg tatggactca cgtttgcacg ttgcgcaagc tctgggcttg
     9541 gcacttgggg atgctcatat cttgcggaat gcaggtggtc gagtgactga agacatgatt
     9601 cgttcgctag ttatttccca gcaacaaatg gggacaagag agattgtggt attgcaccat
     9661 acagactgtg gtgctcagac ctttgaaaat gaaccttttc aggagtattt aaaagaggaa
     9721 ttaggtgtag atgtgtcaga ccaggacttc ttgcccttcc aagatataga agagagtgta
     9781 cgcgaggata tgcaactgct tatcgagtct cccctaatac cagacgatgt cattatctct
     9841 ggtgctattt acaatgttga tacaggaagt atgacagtcg tagaattata aatacttcat
     9901 ttagaaagaa agtgtatgaa gaaaagcagt attttattta tttttatttt attgctatgt
     9961 attggtttac agtatgaaac catctactat acggacggtt cgaggtcagg tgcggaatat
    10021 ggactaatgg gagtttctat ctttctagct ctcttttaca tgattccggc tctttatttt
    10081 ctcttccgta ttgggaaaaa atgggaattg ccaaagaagg ttttgattct gtctttattg
    10141 ggagggatgt tcctttcagg ctggttgtct agttttgcta atacttatat acatgattta
    10201 ctgggggttc ttttcccaga tagtccattt ttaaatgcct ttgaaagtgc tattgtggct
    10261 cctttggtag aagaaccctt gaaattattg ccacttgttt ttgttttggc tttgattcct
    10321 gtgcgaaaat taaaattttt gtttttactt ggaattgcct ccggtttggg attccaaatg
    10381 attgaggata ttggttatat tcgtacggat ttgccagagg gctttgactt tactatttcg
    10441 cgaattttag agcgtatcat ctcaggaatt gcctctcact ggactttttc aggtctagct
    10501 gtagtaggtg tttacttgct ttacagagcc tataaaggac agaaggttgg caagaaacag
    10561 ggtcttattg tttaactctc cttttgtgga gttggaaaca gagttgcctt tagcgattcc
    10621 agtggttacg gctattgctc tctatggtta ttatcatgct tattgctttg ttgagaaaca
    10681 caatgagttg atgacctaga atatttttca aaagaatgat gcaagggtac aaatatggtg
    10741 cccttctttt atttttgatt gaaaaatagt gcaaaaagcg ctacaatggt agatggaaaa
    10801 tcttgtgaaa agcacaagcg atacatatat accggaggaa atcatgtctt tttctgattt
    10861 aaagctgttt gccctttctt ctaataaaga attggcagaa cgtgtggcgc aggagattgg
    10921 gatagagttg gggaaatcaa gtgttcgcca attttcagat ggagagattc aggtcaacat
    10981 tgaagaatca atccgtggga aacacgtctt tatcttacaa tcaactagtt cgcctgtaaa
    11041 tgacaatctg cttgaaattt tgattatggt agatgctttg aagcgtgcga gtgcagagtc
    11101 tgtcaatgtt gtcatgcctt actatgggta tgcacgtcag gatagaaagg cgagagcgcg
    11161 tgagccaatc acttcaaaac ttgtcgcaaa tatgcttgaa gtagctggag tggatcgttt
    11221 attgaccatc gacttgcatg ctgcgcaaat tcaaggattc tttgatattc ctgtggatca
    11281 tttgatgggt gctcctctaa ttgcagatta ttttgagcgg cgtggtatgg ttggttctga
    11341 ctatgtggtt gtcagcccgg accatggagg ggtgactcgt gctcgtaagt tggcagaatt
    11401 tttgaaaaca tctatcgcta ttattgataa acgtcgtagc gttgataaga tgaatactag
    11461 tgaagttatg aacatcatcg gtaaggttga aggcaagact tgtatcttga ttgatgatat
    11521 gattgatacc gctggaacga tttgtcatgc ggcagatgct cttgcggaag ctggtgctgt
    11581 tgcagtctat gcaagctgta cgcacccagt tctttctggt cctgctacgg acaatatcca
    11641 aaaatcagct attaagaaat tggttgtttt ggataccatc tatctgccag aagagcgttt
    11701 gattgataag attgagcaga tttcaatcgc tcatctccta ggggatgcta tcgtacgtat
    11761 tcatgaaaaa cgaccacttt ctccactttt cgatattgag aaaaaaattt aatgaccaag
    11821 cctgagatga ttctcaggtt ttttcgtctt ttttccgaat aaatggatag tagcggtgaa
    11881 tctagtaaag ctagatttaa aactgtgata taataaaaag agaagaaata tgactatacg
    11941 tcatcagaag tttcagcgac catcattttt gaacagtgat agcacttgaa tcgacgcttt
    12001 ctaagg
//
LOCUS       DAVLFB010000061        11772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLFB010000061 DAVLFB010000000
VERSION     DAVLFB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.061"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(127..1757)
                     /locus_tag="VXD58_001450"
                     /pseudo
     CDS             complement(127..1757)
                     /locus_tag="VXD58_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            2050..6960
                     /locus_tag="VXD58_001451"
     CDS             2050..6960
                     /locus_tag="VXD58_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV0676127.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYRFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNK
                     ALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            7050..8246
                     /locus_tag="VXD58_001452"
     CDS             7050..8246
                     /locus_tag="VXD58_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV0676128.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8382..8909
                     /locus_tag="VXD58_001453"
     CDS             8382..8909
                     /locus_tag="VXD58_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0676129.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            8986..9342
                     /gene="glnR"
                     /locus_tag="VXD58_001454"
     CDS             8986..9342
                     /gene="glnR"
                     /locus_tag="VXD58_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV0676130.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9379..10725
                     /gene="glnA"
                     /locus_tag="VXD58_001455"
     CDS             9379..10725
                     /gene="glnA"
                     /locus_tag="VXD58_001455"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV0676131.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10821..10940)
                     /locus_tag="VXD58_001456"
     CDS             complement(10821..10940)
                     /locus_tag="VXD58_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV0676132.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11428..11772
                     /locus_tag="VXD58_001457"
                     /pseudo
     CDS             11428..11772
                     /locus_tag="VXD58_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3764 a         2151 c         2550 g         3307 t
ORIGIN      
        1 agaggtgtac tattctagtt tcaatatact atatatctgc agaatactct ttcagtttca
       61 ttcagcctag tcttgcttag gaaaaagtaa gaagccatcc aattatgatg gatagcttct
      121 tggttattag atttgttctg caaaaacctt ttcagcaagg ttcatagcgt ggtctgatac
      181 acgagtgtag tgtgagatga tgtcgataaa gttgacccca gcttgtgttg aacattcgcc
      241 tttgttgagg cgtttgatgt gggtttttct gagaacacgt tctatcttat tgattgcttc
      301 atgacgttct acaagactgc gtgctttttc aatatcattg ttttccacac tatccagagc
      361 atctttgata aagtcactag tttggcggta aacttcctct aattctttca aggcggcatc
      421 agaaaattca acatttttcc gttgaagata gtcagtcaga ttgagtagag cctccgtgtg
      481 gtctccaatc cgttccaaat cacgggagga atcaaggata ttggtgagca cttcactttc
      541 tttttggctg agagcttcgc ttgaaagggc aatgagataa cgtgttaatt gctcatcgat
      601 ggtgttaatt gcttcttcgg ttttatgccc tttttcagca actttttcat ccaagtcaat
      661 gatgtactta taggaaaggt caaaggcttt agcagcgtag tttcctaagt gcaagagctc
      721 ttttttagca tttcctagag cgatagatgg ggcttgtttg atgaaatgtt catcaagata
      781 taagggttcg tatttgacaa cctcgtcctc tccaggaata atcttggtta caaagtaagc
      841 cagagctccg ataaatggaa attggacaat ggtgttggta atattaaggt tccgtgagca
      901 aaggcgatgg tcatttccgg tgctagattt agcgtagctt caaaccaatg aatcaggaca
      961 gtaaaaggaa ctagaaaaat aacgcagacg actgttccga taacgttgaa ggcaacatga
     1021 gctcctgcta cccgtttagc tgcaatatta gcccctaaag aggcaatgat ggctgtaatg
     1081 gttgtcccga tattgtcacc aaatagaact ggcaaagctc cctgtaggtc aattagattg
     1141 ccggcgtaga ggttttgtaa aatcccaatg gtagccgaag aagcttgaat tagcaaggtc
     1201 aagccggtac cgacaaagac acccaaaaca ggattcttac ttagctcaat catatagtcc
     1261 ttaaagacct gtaaatcctt gagtggagcc attgcgccgc tcatgagatt gagggcaaaa
     1321 aagataccac cgacaccaaa gaggatgcgt ccgatattat tgactgtccg atttttcgta
     1381 aaaaagagac agacggcacc gataaagagc ataggtaggg catagttacc taatttaaaa
     1441 ccgatgagaa aggatgtgac agttgtccca atattagcac ccatgacaat cccgatagcc
     1501 tgacgtaagg ttaagagacc ggcactgacc aggccgactg taataactgt tacaccagaa
     1561 ctagactgaa ttagagcagt catcccaata ccaaccagaa ctccaaagaa aggattacta
     1621 gtatatttgt caatgtaaaa acgaaggcga tctccagcag cttgttgcaa accgtctccc
     1681 atggtcttga tgctatataa gaatagcccc agaccaccta aaaagtgaaa taaaatttcc
     1741 tgccaattaa tggacatttc tttttcctcc gaaaaataat cgcggaatat ctcctattct
     1801 attttaaagg ataaaagtaa atctaacaag tgttaatgtc aagtttgtaa aagaaaatta
     1861 taattgataa gtgtattatt tttaagagtt tttaacctta ttttagattt gtgcaaacag
     1921 ttattgtttt ttgtctaaaa atgttttagg ataaaaatgt gattatcatg gttctagagt
     1981 ttgataaaaa actacagaat ataaagcgct ttctttaaaa caacaaaatc aatcttttag
     2041 gaggagaaaa tgaagaatcc attttttgaa agacgttgtc gttacagtat tcgtaagtta
     2101 tcagtaggag cctgctcgct gatgattggt gctgttttat ttgctggtcc agccttggct
     2161 gaagaaactg cagttcctga aaatagcgga gctaatacag agcttgtttc aggagagagt
     2221 gagcattcga ccaatgaagc tgataagcag aatgaagggg aacatgctag agaaaacaag
     2281 ctagaaaagg cagaagcagc tagcgcagct aaaccagagg aaaaagcagg tgaggtggtt
     2341 gcagaaacac catctgcaga agcaaaacct aagtctgaca aggaaacaga agcaaagccc
     2401 gaagcaacta accaagggga tgagtctaag ccagcagcag aagctaataa gactgaaaaa
     2461 gaagtccagc cagatgtccc taaaaataca gaaaaaacat taaaaccaaa ggaaatcaaa
     2521 tttaattctt gggaagaatt gttaaaatgg gaaccaggtg ctcgtgaaga tgatgctatt
     2581 aaccgcggat ctgttgtcct cgcttcacgt cggacaggtc atttagtcaa tgaaaaagct
     2641 agcaaggaag caaaagttca agccttatca aacaccaatt ctaaagcaaa agaccatgct
     2701 tctgttggtg gagaagagtt caaggcctat gcttttgact attggcaata tctagattca
     2761 atggtcttct gggaaggtct cgtaccaact cctgacgtta ttgatgcagg tcaccgtaac
     2821 ggggttcctg tatacggtac actcttcttc aactggtcta atagtattgc agatcaagaa
     2881 agatttgctg aagctttgaa gcaagacgca gatggtagct tcccaattgc ccgtaaattg
     2941 gtagacatgg ccaagtatta tggctatgat ggctatttca tcaaccaaga aacaactgga
     3001 gatttggtta aacctcttgg agaaaagatg cgccagttta tgctctatag caaggaatat
     3061 gctgctaagg taaaccatcc aatcaagtat tcttggtatg atgccatgac ctataactat
     3121 ggacgttacc atcaagatgg tttgggagaa tacaactacc aattcatgca accagaagga
     3181 gataaggttc cggcagataa cttctttgct aactttaact gggataaggc taaaaatgat
     3241 tacactattg caactgccaa ctggattggt cgtaatcctt atgatgtatt tgcaggtttg
     3301 gaattgcaac agggtggttc ctacaagaca aaggttaagt ggaatgacat tttagacgaa
     3361 aatgggaaat tgcgcctttc tcttggttta tttgccccag ataccattac aagtttagga
     3421 aaaactggtg aagattatca taaaaatgaa gatatcttct ttacaggtta tcaaggagac
     3481 cctactggtc aaaaaccagg tgacaaagat tggtatggta ttgctaacct agttgcggac
     3541 cgtacgccag cggtaggtaa tacttttact acttctttta atacaggtca tggtaaaaaa
     3601 tggttcgtag atggtaaggt ttctaaggat tctgagtgga attatcgttc agtatcaggt
     3661 gttcttccaa catggcgctg gtggcagact tcaacagggg aaaaacttcg tgcagaatat
     3721 gattttacag atgcctataa tggcggaaat tcccttaaat tctctggtga tgtagccggt
     3781 aagacagatc aggatgtgag actttattct actaagttag aagtaactga gaagaccaaa
     3841 cttcgtgttg cccacaaggg aggaaaaggt tctaaagttt atatggcatt ctctacaact
     3901 ccagactaca aattcgatga tgcagatgca tggaaagagc taaccctttc tgacaactgg
     3961 acaaatgaag aatttgatct cagctcacta gcaggtaaaa ccatctatgc agtcaaacta
     4021 tttttcgagc atgaaggtgc tgtaaaagat tatcggttca acctaggaca attaactatc
     4081 tcggacaatc accaagagcc acaatcgccg acaagctttt ctgtagtgaa acaatctctt
     4141 aaaaatgccc aagaagcgga agcagttgtg caatttaaag gcaacaagga tgcagatttc
     4201 tatgaagttt atgaaaaaga tggagacagc tggaaattac taactggctc atcttctaca
     4261 actatttatc taccaaaagt tagccgctca gcaagtgctc agggtacaac tcaagaactg
     4321 aaggttgtag cagtcggtaa aaatggagtt cgttcagaag ctgcaaccac aacctttgag
     4381 tgggggatga ctgtccaaga cacaagtctt ccaagacctc tagcagaaaa catcgttcca
     4441 ggggctactg ttatcggaag taccttccca gatacagaag gtggagaagg tattgaaggt
     4501 atgttgaacg gtaccattac tagcttgtca gataaatggt cttcagctca gttgagtggt
     4561 agtgtggata ttcgtttgac caagccacgt accgttgtta gatgggtcat ggatcatgca
     4621 ggggctggtg gtgagtctgt taacgatggc ttgatgaaca ccaaagactt tgacctttat
     4681 tataaagatg cagatggtga gtggaagcta gctaaggaag tccgtggcaa caaagcacac
     4741 gtgacagata tcactcttga taaaccaatc actgctcaag actggcgctt gaatgttgtc
     4801 acttctgaca atggaactcc atggaaggct attcgtatct ataactggaa aatgtatgaa
     4861 aagcttgata ctgagagtgt caatattccg atggccaagg ctgcagcccg ttctctaggc
     4921 aataacaagg tacaagttgg ctttgcagat gtaccggctg gagcaactat taccgtttat
     4981 gataatccaa attctcaaac tccactcgca accttgaaga gcgaagttgg aggagaccta
     5041 gcaagtgcac cattggattt gacaaatcaa tctggtcttc tttattatcg tacccagttg
     5101 ccaggcaagg aaattagtaa tgtcctagca gtttccgttc caaaagatga cagaagaatc
     5161 aagtcagtca gcctagaaac aggacctaag aaaacaagct acgccgaagg ggaggatttg
     5221 gaccttagag gtggtgttct tcgagttcag tatgaaggag gaactgagga cgaactcatt
     5281 cgcctaactc acgcaggtgt atcagtatca ggttttgata cgcatcataa gggagaacag
     5341 aatcttactc tccaatattt gggacaacca gtaaatgcta atttgtcagt gactgtcact
     5401 ggccaagacg aagcaagtcc gaaaactatt ttgggaattg aagtaagtca ggaaccgaaa
     5461 aaagattacc tagttggtga tagcttagac ttgtctgaag gacgctttgc agtggcttat
     5521 agcaatgaca ccatggaaga acattccttt actgatgagg gagttgaaat ttctggttac
     5581 gatgctcaaa agactggtcg tcaaaccttg acgcttcgtt accaaggtca tgaagtcaac
     5641 tttgatgttt tggtatctcc aaaagcagca ttgaacgatg agtacctcaa acaaaaatta
     5701 gcagaagttg aagctgctaa gaacaaggtg gtctataact ttgcttcacc agaagtaaaa
     5761 gaagctttct tgaaagcaat tgaagcggcc gaacaagtgt tgaaagacca tgaaactagc
     5821 acccaagatc aagtcaatga ccgacttaat aaattgacag aagctcataa agctctgaat
     5881 ggtcaagaga aatttaagga agaaaagaca gagcttgatc gtttaacagg tgaggttcaa
     5941 gaactcttgg ctgccaaacc aaaccatcct tcaggttctg ccctagctcc gcttcttgag
     6001 aaaaacaagg ccttggttga aaaagtagat ttgagtccag aagagcttgc aacagcgaaa
     6061 cagagtctaa aagatctggt tgctttattg aaagaagaca agccagcagt cttttctgat
     6121 agtaaaacag gtgttgaagt acacttctca aataaagaga agactgtcat caagggtttg
     6181 aaagtagagc gtgttcaagc aagtgctgaa gagaagaaat actttgctgg agaagatgct
     6241 catgtctttg aaatagaagg tttggatgaa aaaggtcaag atgttgatct ctcttatgct
     6301 tctattgtga aaatcccaat tgaaaaagat aagaaagtta agaaagtatt tttcttacct
     6361 gaaggcaaag aggcagtaga attggctttt gaacaaacgg atagtcatgt tatctttaca
     6421 gcacctcact ttactcatta tgcctttgtt tatgaatctg ctgaaaaacc acaacctgct
     6481 aaaccagcac cacaaaacac agtccttcca aaacctactt atcaaccggc ttctgatcaa
     6541 caaaaggctc ctaaattgga agttcaagag gaaaaggttg cctttcatcg tcaagagcat
     6601 gaaaatgctg agatgctagt tggggaacaa cgagtcatca tacagggacg agatggactg
     6661 ttaagacatg tctttgaagt tgatgaaaac ggtcagcgtc gtcttcgttc aacagaagtc
     6721 atccaagaag cgattccaga aattgttgaa attggaacaa aagtaaaaac agtaccagca
     6781 gtagtagcta cacaggaaaa accagctcaa aatacagcag ttaaatcaga agaagcaagc
     6841 aaacaattgc caaatacagg aacagctgat gctaatgaag ccctaatagc aggcttagcc
     6901 agccttggtc ttgctagttt agccttgacc ttgagacgga aaagagaaga taaagattaa
     6961 atatcgaaaa atcttgtgaa atctttcctt atatttctaa agtgtgatat aatagtttcg
     7021 aataaaataa ataaaggagt ttttgtaaca tggcaaaact tactgttaaa gacgttgact
     7081 tgaaaggtaa aaaagtcctc gttcgtgttg acttcaacgt accattgaaa gatggcgtaa
     7141 tcactaacga taaccgtatc acagcagctc ttccaactat taagtacatc atcgaacaag
     7201 gtggacgtgc aattcttttc tctcaccttg gacgtgtgaa agaagaagct gataaagctg
     7261 gtaaatcact tgctcctgta gcagctgact tggcagcaaa acttggtcaa gatgttgttt
     7321 tcccaggtgt cactcgtggt gctgaattgg aagcggcaat caacgctctt gaagatggac
     7381 aagttctctt ggttgaaaac actcgttacg aagatgttga cggcaagaaa gaatctaaaa
     7441 acgatcctga acttggtaaa tactgggcat cacttggaga tggtatcttc gtaaacgatg
     7501 cattcggtac agctcaccgt gcacacgcat ctaacgttgg tatctcagca aacgttgaaa
     7561 aagcagttgc tggtttcctt cttgaaaacg aaattgccta catccaagaa gcagttgaaa
     7621 ctccagaacg tccattcgtg gctatccttg gtggttcaaa agtttcagac aagatcggtg
     7681 ttatcgaaaa cttgcttgaa aaagctgata aagtccttat cggtggtggg atgacttaca
     7741 cattctacaa agcacaaggt atcgaaatcg gtaactcact tgtagaagaa gacaaattgg
     7801 atgttgcgaa agctcttctt gaaaaagcaa atggtaaatt gatcttgcca gttgactcaa
     7861 aagaagctaa cgcatttgct ggttacactg aagtgcgtga cactgaaggt gaagcagttt
     7921 ctgaaggctt ccttggtctt gacatcggtc caaaatctat cgccaaattt gacgaagctt
     7981 tgactggtgc caaaacagtt gtatggaacg gacctatggg tgtatttgaa aacccagatt
     8041 tccaagctgg tacaatcggt gtgatggacg ctatcgtgaa acaaccagga gttaaatcaa
     8101 tcatcggtgg tggtgactca gctgccgcag cgattaacct tggccgtgca gacaagttct
     8161 catggattag tacgggtggt ggagcatcaa tggaacttct tgaaggtaag gttcttccag
     8221 gacttgcagc cttgacagaa aaataagatt ttataaacaa atcaaagaag agagggatga
     8281 aagttcctct tttcttttgc ttaaaataaa aacgcttcct ctcaactatt actcataaaa
     8341 atcaccgatt tatgataaaa tggaaataga aagttgagat tatgagttat tttaaaaaat
     8401 ataaattcga taaatcccag ttcaaacttg gtatgcgaac ctttaaaaca ggtattgctg
     8461 tttttctagt tctcttgatt tttggctttt ttggctggaa aggtcttcaa attggtgctt
     8521 tgacagccgt ttttagcctg agggagagtt ttgatgagag tgttcatttt gggacttcgc
     8581 gtattctagg aaatagtatc ggtggactct atgccttggt cttcttctta ttaaatacct
     8641 ttttccacga agccttttgg gtgaccttgg tagttgttcc aatctgcacc atgttaacca
     8701 ttatgacaaa tgtagccatg aataacaaag caggggttat tggtggtgta gcagctatgt
     8761 taattattac cctatcaatt ccaagtggtg agacaatttt gtacgtgttt gtgcgtgtat
     8821 tagaaacatt tatgggagtt tttgtcgcaa ttatcgtaaa ttacgatatt gatcgtattc
     8881 gtctcttttt agagaaaaaa gaaaaataat gttacatttt ataacattat taattgacgt
     8941 ttgtcttttt ttagactata atagacagaa agaaggaaat tgtaaatgaa ggaaaaagaa
     9001 tttcgccgaa atatggctgt ttttcctatc ggcagtgtta tgaagttgac cgatctatcg
     9061 gcgcgtcaga ttcgttatta tgaaaatcaa gagttgatca agcccgatcg aaacgaagga
     9121 aatcgtcgca tgtattcctt gaatgacatg gatcgtctgc ttgaaatcaa agattatatc
     9181 tctgaaggtt ataatatcgc tgccattaag aaaaaatatg ctgaacgtga agcgaaatcc
     9241 aagaaagcgg ttagtcagac tgaagtacgt cgtgcacttc acaatgaact cctccaacag
     9301 gggcgttttg cttcagtaca gtcacctttt ggtcgcggtt aggcaaccgc aagtagtcat
     9361 agattaagga gaaaacttat gccaatcaca gctgcagata ttcgtcgtga agtcaaggaa
     9421 aaaaatgtta cctttattcg tcttatgttc tcagatattt tgggaaccat gaaaaacgtc
     9481 gaaattcctg ctacagatga acagttagat aaggtcttgt cgaacaaggt tatgtttgat
     9541 ggatcttcta ttgaaggttt tgtacgtatc aatgagtcgg atatgtactt gtacccggac
     9601 ttggatacat ggacagtctt cccttgggga gatgaaaatg gaagtgttgc aggtctgatc
     9661 tgtgatgttt atacaacaga aggtgaacca tttgcgggtg accctcgtgg taatttgaaa
     9721 cgagctcttc gtcacatgga agaagttgga ttcaaatcct tcaaccttgg tccagagcca
     9781 gaattcttcc tatttaagtt ggatgaaaat ggggacccaa cacttgaagt gaatgacaag
     9841 ggtggctact ttgatttggc acctactgac cttgcggaca acacacgtcg tgagattgtg
     9901 aatgtcttga ccaaaatggg atttgaagta gaagcgagtc accacgaggt tgcggttgga
     9961 cagcatgaga ttgactttaa gtacgatgaa gttctccgtg cttgtgataa gattcaaatc
    10021 tttaaacttg ttgttaaaac cattgctcgc aaacacggac tttacgcaac atttatggcg
    10081 aagccaaaat ttggtattgc tggctcaggt atgcactgta atatgtcctt gtttgatgca
    10141 gaaggaaata acgccttctt tgatccaaat gatccaaaag gaatgcagtt gtcagaaaca
    10201 gcttaccatt tcctaggcgg tttgatcaag catgcttaca actatactgc catcatgaac
    10261 ccaacagtta actcatacaa acgtttggtt ccaggttatg aagcgcctgt ttacattgct
    10321 tgggctggtc gtaaccgttc gccacttgtg cgcgtacctg cttcacgtgg tatgggaact
    10381 cgtcttgagt tgcgttcagt ggatccaatg gcgaaccctt acgttgctat ggctgttctt
    10441 ttggaagttg gtttgtatgg tattgaaaat aaaatcgaag caccagctcc tatcgaagaa
    10501 aatatctaca tcatgacagc agaagagcgc aaggaagctg gtattacaga ccttccatca
    10561 actcttcaca acgctttgaa agctttgaca gaagatgaag tggttaaagc tgctctcgga
    10621 gatcacatct atactagctt ccttgaagcc aaacgaatcg aatgggcaag ttatgcaacc
    10681 ttcgtttcac aatgggaaat tgataattat ttagaccttt actaatacta atatagaaga
    10741 aagattgcct gtgggtaaac agtcacacag cagtctaagc catcaaatac ctataaccct
    10801 taaaatagcg atttcggtcg ctatttattt ttcgaaaaat cctcttgact cttttgcgaa
    10861 ttagatttat catcagacgt gtagaagaaa attaaggagt cactatggaa aaattcaatt
    10921 tcaaaaacaa tatcgggcaa gaaaacaagt tacttcaaat cgaaatctac aagtttacta
    10981 acttttgcaa gcttcaaaat tacacatctg taaatatttt ttcgaaagat atatttgaag
    11041 ccatagtaaa ttgagactag aatagtacac tacatctgct aaaaatttct agaaactaat
    11101 ttaactttcc taatcgattt gtttacatct tatttcaatc tactatatta tctcaataaa
    11161 tgatgatggc gactgttttg tttgtgcttg tcgatttttg aatcctgatc gagcggaaga
    11221 atatttctgc cgaggttgcc aaggggaaaa tcctgaagat attgaatttt atgatgaaca
    11281 attacaggca gaaaaggtgg aggttttgaa tattcgattg gaagtaaaaa attgaattat
    11341 ctacaatctc atcaacaata aacctatttc aaccccatta tctataggcg tatttttacg
    11401 cctttttata tacttgaagc acataagaaa cattatggga agtttgctga tggaagtact
    11461 caagaaattg atgttcctta tgatattcct gatacttggg agtgggtgag gataaaatca
    11521 atttattgga attttgggca aaataagcca gagaaatcct ttaggtatat agatacgtct
    11581 agtattgata gaaaaaagaa cataatcaac tacaaaaatc tacaatatct ttcacctgaa
    11641 caagcgcctt cccgtgctag aaaattagtt tcgcagaata gtgtcttatt ttcaacagtt
    11701 agaccatatc taaaaaatat tgctgtagtt agagaactta aagagtattt gatagctagt
    11761 acagcattta tt
//
LOCUS       DAVLFB010000062        11653 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLFB010000062 DAVLFB010000000
VERSION     DAVLFB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11653
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.062"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VXD58_001458"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VXD58_001458"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0676133.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VXD58_001459"
     CDS             complement(1169..1693)
                     /locus_tag="VXD58_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV0676134.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLIEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VXD58_001460"
     CDS             complement(1746..1982)
                     /locus_tag="VXD58_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV0676135.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSTKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VXD58_001461"
     CDS             complement(2050..2970)
                     /locus_tag="VXD58_001461"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV0676136.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3563
                     /locus_tag="VXD58_001462"
                     /pseudo
     CDS             3160..3563
                     /locus_tag="VXD58_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3779..4360
                     /locus_tag="VXD58_001463"
     CDS             3779..4360
                     /locus_tag="VXD58_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0676137.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4357..5634
                     /locus_tag="VXD58_001464"
     CDS             4357..5634
                     /locus_tag="VXD58_001464"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV0676138.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5652..6314
                     /gene="deoC"
                     /locus_tag="VXD58_001465"
     CDS             5652..6314
                     /gene="deoC"
                     /locus_tag="VXD58_001465"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV0676139.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGPDMGVKAAGGARSYADALTFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6301..6690
                     /locus_tag="VXD58_001466"
     CDS             6301..6690
                     /locus_tag="VXD58_001466"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV0676140.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6781..7833
                     /locus_tag="VXD58_001467"
     CDS             6781..7833
                     /locus_tag="VXD58_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV0676141.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7977..9512
                     /locus_tag="VXD58_001468"
     CDS             7977..9512
                     /locus_tag="VXD58_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676142.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGAINADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            9505..10563
                     /locus_tag="VXD58_001469"
     CDS             9505..10563
                     /locus_tag="VXD58_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676143.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10566..11522
                     /locus_tag="VXD58_001470"
     CDS             10566..11522
                     /locus_tag="VXD58_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676144.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3329 a         2082 c         2661 g         3581 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcgattaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgtgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agttgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc atcttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ctactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatatag
     3061 tataagtgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg aaccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgttc ttaattgctt tcttactaaa aaacgatgga
     3361 cgaaatggat tgctgcgggt agttattgct ttttagtcct acggattgcg acagagggtt
     3421 atttcttcca catgtcacta gaagatttga tacgactttg gacaagtttg gaaatcctga
     3481 cacaaacgta tcagttgggc ttttatctaa acattttgtt aggaattttg ttgataataa
     3541 agtattttaa ggttaagcaa tagaaataat aatctgcctg attatagatg ataaactttt
     3601 tgtttttatc tggactgtga tatttatagt tctaaatggt ttattattta taagtgttga
     3661 aagagattga agaaaatggc ttcgtgactt gctttcttcg gtctttttcg ttttattatc
     3721 aatttgtaaa cgatttacaa tttgagtcaa attatggtaa aatagtgtca tgagtaaaat
     3781 gtactatgca gaaaatcctg acgctgctca cgacattcat gagttgagag tggacttgtt
     3841 gggagaaaaa atgacttttt tgacagatgc gggtgttttt agcaagaaaa tggttgactt
     3901 tggaagtcaa ctcttgctca agtgtctgga tgtcaaccaa ggagagacag tcctagatgt
     3961 aggctgtggt tatggtccat taggtttgtc cttggtcaag gtttacggag ttcaggcgac
     4021 catggttgat attaacactc gtgccctgga tttagctcag agaaatgctg aaaaaaataa
     4081 tgcaaaagcg acgatatttc aatctaatat ttatgcaaga gttcaagggc gatttgacca
     4141 tgtcatttcc aatcctccta tccgtgcggg caagcaagtc gttcatgaaa tcatcgagaa
     4201 aagtaaagat ttcttggaaa ttggtggaga tttgaccatc gtcatccaga aaaaacaagg
     4261 agctccaagt gccaaatcta agatggaaga tgtgtttggc aattgtgaaa tccttaaaaa
     4321 agacaaggga tactatatcc ttagaagtgt gaaagaatga gagcagttga tttaatccaa
     4381 aaaaaacgag acggtcaaga actgacttca agtgaaattg aatggctgat agaaggctat
     4441 gtatcaggaa ctgttcctga ttatcagatg tctgcctttg ctatggctgt ttattttaaa
     4501 ggaatgacga ctcgagaaat ctctgattta acgatgaata tggttaagac tgggcaagag
     4561 tttgacctgt cagccattga tggtgtgaaa gttgacaagc attctactgg tggtgtcggc
     4621 gataaggtaa ccttgatttt agctcctctt gttgctagct tcggtgtgcc tgtagcaaaa
     4681 atgagtggtc gtggtcttgg ccatacaggc ggaacaattg ataaattgga gtccattagg
     4741 ggctatcaag tagaacgtag tcaagaggat tttattcgtc aggttcagga tattggagta
     4801 tctgtcattg ggcaatcaga ccagctggtc aaagcagata agcttctcta tgcccttcgt
     4861 gatgtgaccg caactgtcga cacgattcct ttgattgcga gttcggtgat gagcaagaaa
     4921 attgcggcag gggcggatgc tattttgcta gacgtgactg tcggtgaggg tgccttcatg
     4981 aagacggttg atgaggcgcg tgagttggct caaactatgg tagatcttgg taaggttgtt
     5041 ggacggaaga cagtagcagt gattacggat atgagccagc ctttggggcg agcgattgga
     5101 aatcgtctgg aaatccttga agcattggag attttgcaag gtcaaggacg tcaggatatt
     5161 acccacttta tttgtgaatt ggctcaaatt atgcttggtt tggcaaatgt taacaaaact
     5221 gttgaagaag ttcgccaaca tcttgaaaat ggtcaagcac tggctaagtt tgaggaaatg
     5281 gtgcaagccc aaggtggaga cttggaagac ctctatcgtc ctgtaaatgt agctcatgtg
     5341 gtggaaatcc ctgctcagga aacgggtgtc atttcagctc ttccagctat ggactttggt
     5401 ctttatgcca tgaggctggg tgctggtcgt gcagttaagt ctgatgtctt ggactatgaa
     5461 acaggaattg tttttgaaaa gaagattgga gactccgttc aaaagggtga aattgttgca
     5521 aaagtataca caaatgaaaa aattccacct caactagtta cagatttcca aaaatgtgtt
     5581 aaaataagtg atgaagtgaa aaaaatccgt gaaattgtag aaatcatctc ctaaatggag
     5641 ggaaaaccga aatgaaatta aataaatata tagatcatac gcttttaaaa caagatgcaa
     5701 agaaaaaaca aattgatagt ttgttgtctg aggctagaga gtatggcttt gccagtgttt
     5761 gcgttaatcc gacctgggtt gaacatgcta aaaaaggact tgaaggcaca gatgttaagg
     5821 tttgcacagt agtaggtttc cccttgggag caacaacttc aactgtgaaa gcttttgaaa
     5881 caaaagaagc tatccaaaat ggggctgatg agattgacat ggtaatcaat gttggggctc
     5941 tcaagtcagg tgatttggcc ttggttgagt cggatattcg tgcagtagtg gaagcaagtg
     6001 gggacaagtt agtgaaagtt attattgaag cttgccttct gacagaccaa gaaaaagttt
     6061 tggcttgcca attagcccaa aaagcaggag ctgactttgt caaaacatct actggctttt
     6121 caactggtgg tgctacgata gcagatgtta gattaatgcg tgaaacagtt ggacctgata
     6181 tgggtgtcaa ggccgccggt ggagctcgtt cttatgcaga tgctcttacc tttgtcgaag
     6241 caggtgcgac ccgtatcgga acgtcagctg gggtagctat tttaaaagga gaattggcag
     6301 atggcgacta ctgagttgat tgaactggca attgaaacca gcaaacatgc ctatgtcccc
     6361 tattctcact ttcctatcgg agcagtttta gtagccaaag acggaagtgt ttacacggga
     6421 gtgaatatcg aaaatgctag ctatcctttg accaattgcg gtgagagaac agctattttt
     6481 aaggctatat ctgaaggcca aagagaattt tcagaattga ttgtctatgg tcaaactgaa
     6541 aaaccgattt caccatgtgg tgcttgtcgc caagtaatgg tcgaattttt tgaacaagat
     6601 ttaaaagtga ccctagtcgc aaaagataaa tcgacggtcg agatgacggt cggggagtta
     6661 cttccatact cttttacaga cttaaattag tctgagtcgc tctttgagtg gcaagggtct
     6721 ttgtgaccaa tcaatccata cttgcaacat cgttgcacat cttatttagg aggttcagta
     6781 atgaacaaga aacaatggct aggtcttggc ctagttgcag tggcagcagt tggacttgct
     6841 gcatgtggta accgctcttc tcgtaacgca gcttcatctt ctgatgtgaa gacaaaagca
     6901 gcaatcgtca ctgatactgg tggtgttgat gacaaatcat tcaaccaatc agcttgggaa
     6961 ggtttgcaag actggggtaa agaacacaat ctttcaaaag ataacggttt cacttacttc
     7021 caatcaacaa gtgaagctga ctacgctaac aacttgcaac aagcggctgg aagttacaac
     7081 ctaatctttg gtgttggttt tgcccttcac aatgcagttg aagaagcagc taaagaacac
     7141 actgacttga actatgtctt gattgatgat gtgattaaag accaaaaaaa tgttgcgagc
     7201 gtaactttcg ctgataatga gtcaggttac cttgcaggtg tggctgcagc aaaaacaact
     7261 aagacaaaac aagttggttt tgtaggtggt atcgaatctg aagttatctc tcgttttgag
     7321 gcaggattca aggctggtgt tgcgtcagta gacccatcta tcaaagtaca agttgactac
     7381 gctggttcat ttggtgatgc ggctaaaggt aaaacaattg cagccgcaca atacgcagcc
     7441 ggtgcagata ttgtttacca agtagctggt ggtacaggtg caggtgtctt tgcagaggca
     7501 aaatctctca acgaaagccg tcctgaaaat gaaaaagttt gggttatcgg tgttgaccgt
     7561 gaccaagaag cagaaggtaa atacacttct aaagatggca aagaatcaaa ctttgttctt
     7621 gtatctactt tgaaacaagt tggtacaact gtaaaagata tttctaacaa ggcagaaaaa
     7681 ggagaattcc ctggcggtca agtgatcgtt tactcattga aggataaagg ggttgacttg
     7741 gcagtaacaa acctttcaga agaaggtaaa aaagctgtcg aagatgcaaa agctaaaatc
     7801 cttgatggaa gcgtaaaagt tcctgaaaaa taatggatgg aagtcatttc ttaggaagcg
     7861 gccaagggcc gcttttttaa gagttgagac aaagtcattt tgtctcagta aagcttgcta
     7921 ctagacaaac tagcaagttt tattgaaata aaataagact ctgaaaggaa gagcacatgg
     7981 cacacgaaaa tgtcattgag atgcgtgata ttaccaaggt gtttggtgga tttgttgcca
     8041 acgacaaaat caacttgcac ctacgaaaag gtgaaattca tgcactttta ggagaaaatg
     8101 gggctggtaa gtccacgcta atgaacatgt tagcaggcct tcttgaacca accagtggtg
     8161 aaatcgcggt caacggtcaa gttgtcaatc tcgactcccc atctaaagca gctagcttgg
     8221 gaatcgggat ggttcaccag cactttatgt tggttgaagc cttcacagtg gctgaaaaca
     8281 tcattttagg tagtgaattg actaaaaatg gtgtgctaga tatcgctgga gctagcaaag
     8341 aaatcaaggc tctttctgaa cgttatggct tagctgttga cccttctgcc aaggtagcag
     8401 atatctcagt tggagcccaa caacgtgtag aaattttaaa aacactttat cggggggctg
     8461 atatccttat ctttgacgaa ccaacggctg ttttgactcc atcagaaatt gatgagttga
     8521 tggctattat gaaaaatctt gtcaaagaag gaaaatcaat tatcttgatt acccacaaat
     8581 tggatgaaat tcgcgcagtt tctgaccgtg ttacagttat ccgtcgtggg aaatcaattg
     8641 aaaccgttga aattgcaggg gctatcaatg ctgatttggc ggaaatgatg gtaggacgtt
     8701 ctgtttcctt taaaacagag aagcaagcct ctaaaccaaa agaagtggtt ttgtctatca
     8761 aagatttggt ggtcaatgaa aaccgtggtg ttccagctgt taaaaatctg tccttggatg
     8821 ttcgtgctgg agagattgtt ggtattgcgg ggattgatgg aaatggtcag tctgaactga
     8881 ttcaagccat tacaggtctt cgtaaggttg aatctggtag cattgagcta aaaggagatt
     8941 caattgtagg cttgcaccca cgtcagatta cagaactaag tgttgggcac gttccagaag
     9001 accgtcaccg tgatggcttg attttggaaa tgatgatatc tgaaaatatt gcccttcaaa
     9061 cctactataa agaaccacat agtaaaaatg gaattttgaa ttattcaaat attacttctt
     9121 atgctaaaaa gctgatggaa gagtttgatg ttcgcgctgc cagtgaatta gttcctgcag
     9181 ctgcactctc aggaggaaat caacaaaaag caattattgc tcgtgaaatt gatcgagatc
     9241 ctgatctcct tatcgttagc cagccaactc gtgggttgga tgtcggtgcc attgagtata
     9301 tccacaaacg cttgattgaa gagcgtgata atggcaaggc tgtccttgtt gtcagctttg
     9361 aattggatga gattttaaac gtctcagacc gtattgccgt tatccacgat ggtaagattc
     9421 aaggtattgt atcaccagaa acaaccaata aacaaggact tggtgtcttg atggctggtg
     9481 gaaacttggg aaaggagaag agtgatgtct aaaaaattac aacaaatttc ggttcccttg
     9541 atttctgtat tcctaggaat tttactcgga gccattgtca tgtggatctt cggttatgat
     9601 gctatttggg gctacgaaga attgttctat acagcctttg gcagtctgcg tgggattgga
     9661 gaaatcttcc gtgctatggg tcctctggtc ttgattggtc ttggttttgc ggttgccagt
     9721 cgagctggtt tctttaacgt cggacttcct ggtcaggctt tggcaggttg gattctcagt
     9781 ggttggtttg ccctgtcgca tccagatatg ccccgtccct tgatgattct agcaaccatc
     9841 gtgattgcct tgattgctgg tgggattgtc ggagcgattc caggtattct tagggcctat
     9901 ctagggacgt cagaggttat tgtaaccatc atgatgaact acattgtctt gtatgtaggg
     9961 aatgccttta tccatgcttt ccctaaagac ttcatgcaaa gtacagattc gaccattcgt
    10021 gttggggcta atgcaaccta tcagacacct tggttagctg agttgactgg taactcacgg
    10081 atgaatattg gtattttctt tgccatcatt gccgttgcag ttatttggtt catgctcaag
    10141 aaaacaactc ttggttttga aatccgtgca gttggtctta atccacatgc ttcagaatat
    10201 gctggtattt ctgccaagcg gactattatc ctatctatga ttatttcagg tgccttggca
    10261 ggtcttggtg gagctgttga aggtttggga accttccaga acgtctatgt tcaaggttcg
    10321 tcattagcta tcggatttaa cggaatggcg gttagtttgc ttgcggccaa ctcaccaatt
    10381 ggtatactct ttgcagcctt cctatttggc gttctccaag ttggggctcc tggtatgaat
    10441 gcggcgcagg taccatctga gctcgtcagc attgtaacag cgtctattat cttctttgtc
    10501 agtgttcatt accttatcga acgctttgtc aaaccgaaaa aacaagttaa aggaggtaag
    10561 taaagatgtc tattataacc ttgctccccc tcttggtgtc ttctatgctg atttactcag
    10621 cacctctcat ctttacaagt atcggtggtg ttttctctga acgtggtggt gtggtaaacg
    10681 ttggccttga aggaattatg gttatgggtg ccttttctgg agttgtcttt aaccttgaat
    10741 ttgcagaaca atttggagca gcaactccat ggctatcctt gcttgtagca ggattggttg
    10801 gtagtgtttt ctctatcatc cacgcagcag cgacggttca tttccgtgca gaccatgttg
    10861 tcagcggtac ggtattgaac ttgatggcgc ctgccttggc tgttttctta gttaaagttc
    10921 tttataacaa aggacaaacc gacaacctaa gtcaaacttt tggacgcttt gatttcccag
    10981 tcttggcaaa tatcccagtg attggtgata tcttcttcaa gtcaactagt ctacttggtt
    11041 atctggcgat tgccttctca ttccttgctt ggtttattct ctttaagact caatttggtc
    11101 ttcgtctccg ctctgtcggt gaacatcctc aagcagcgga taccttggga atcaacgtct
    11161 acaagatgag atatttaggg gttattattt caggttttct aggtggaatt ggcggagcga
    11221 tttatgcgca atcaatctca gttaacttct cagtgacaac tattgttgga cctggattta
    11281 tcgcccttgc tgcgatgatc tttgggaaat ggaatccaat cggtgctatg ctttctagtc
    11341 tcttctttgg actttcacaa agtttggctg ttatcggttc tcaattgccg ttcctacaag
    11401 gagtgccagc ggtttatctt caaattgcac cttatgtttt gacaattctt gtcttagcag
    11461 ccttctttgg aaaagcagtc gcaccaaaag cagatggtat caactacatc aaatcaaaat
    11521 aagcatacaa aaaaacgtca gtccaatgac tgaactgccc cccaaaagtt agacagaaaa
    11581 aatctaactt ttggggtgtt tttattatga aattaactta tgatgataaa gttcagatct
    11641 atgaacttag aaa
//
LOCUS       DAVLFB010000063        11630 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLFB010000063 DAVLFB010000000
VERSION     DAVLFB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.063"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            116..874
                     /locus_tag="VXD58_001471"
     CDS             116..874
                     /locus_tag="VXD58_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0676145.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            914..1678
                     /locus_tag="VXD58_001472"
     CDS             914..1678
                     /locus_tag="VXD58_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV0676146.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     EFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1691..2368
                     /locus_tag="VXD58_001473"
     CDS             1691..2368
                     /locus_tag="VXD58_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0676147.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2378..3037
                     /locus_tag="VXD58_001474"
     CDS             2378..3037
                     /locus_tag="VXD58_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0676148.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3332..4522
                     /locus_tag="VXD58_001475"
     CDS             3332..4522
                     /locus_tag="VXD58_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV0676149.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTIKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4843..5724)
                     /locus_tag="VXD58_001476"
     CDS             complement(4843..5724)
                     /locus_tag="VXD58_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV0676150.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5865..7193)
                     /gene="vncS"
                     /locus_tag="VXD58_001477"
     CDS             complement(5865..7193)
                     /gene="vncS"
                     /locus_tag="VXD58_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV0676151.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(7190..7846)
                     /gene="vncR"
                     /locus_tag="VXD58_001478"
     CDS             complement(7190..7846)
                     /gene="vncR"
                     /locus_tag="VXD58_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV0676152.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7895..7978)
                     /gene="pep27"
                     /locus_tag="VXD58_001479"
     CDS             complement(7895..7978)
                     /gene="pep27"
                     /locus_tag="VXD58_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV0676153.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8158..9537)
                     /gene="vex3"
                     /locus_tag="VXD58_001480"
     CDS             complement(8158..9537)
                     /gene="vex3"
                     /locus_tag="VXD58_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV0676154.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9589..10236)
                     /gene="vex2"
                     /locus_tag="VXD58_001481"
     CDS             complement(9589..10236)
                     /gene="vex2"
                     /locus_tag="VXD58_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV0676155.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10249..11526)
                     /locus_tag="VXD58_001482"
     CDS             complement(10249..11526)
                     /locus_tag="VXD58_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676156.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3263 a         2604 c         2213 g         3550 t
ORIGIN      
        1 gatcgatttt tcctgttctt atttcactat agtgaatttt ttcagatatg gtttgacaag
       61 aacattcttt tattgcagaa ttgtgttata aaatctaaca taaaggagtt ctctcatggc
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac tccgcaacat
      181 caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg gctctgggaa
      241 gtccactctt atccgtacga tcaatggttt agaggctgtt gacaaaggaa gtctcctagt
      301 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt tttcaacatt ttaaccttta tccacacaaa acagtgttag aaaacgtgac
      421 acttgcaccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgccaaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcgtgatg ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
      781 agatgtcgat aacttttttg acaatccaag cgaacctcgt gcccaacaat tcctcagcaa
      841 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaaattctt
      901 tttatcagca ttattgatta gccttttcgg ccttgctgct gccaaaccag tccaggctga
      961 tacaagtatc gcagacattc aaaaaagagg cgaactggtt gtcggtgtca aacaagacgt
     1021 tcccaatttt ggttacaaag atcccaagac cggtacttat tctggtatcg aaaccgactt
     1081 ggccaagatg gtagctgatg aactcaaggt caagattcgc tatgtgccgg ttacagcaca
     1141 aacccgcggc cccctcctag acaatgaaca gggcgatatg gatatcgcga cctttaccat
     1201 cacggacgaa cgcaaaaaac tctacaactt taccagtccc tactacacag acgcttctgg
     1261 atttttggtc aataaatctg ccaaaatcaa aaagattgag gacctaaacg gcaaaaccat
     1321 cggagtcgcc caaggttcta tcacccaacg cctgattact gaactgggta aaaagaaagg
     1381 tctgaagttt aaattcgtcg aacttggttc ctacccagaa ttgattactt cccttcacgc
     1441 tcatcgtatc gatgcctttt ccgttgaccg ctctattcta tctggctaca ctagtaaacg
     1501 gacagcacta ctagatgata gtttcaagcc atctgactac ggtattgtta ccaagaaatc
     1561 aaatacagag ttcaacgact atcttgataa cttggttact aaatggagca aggatggtag
     1621 tttgcagaaa ctttatgatc gttacaagct caaaccatct agccatactg cagattaagg
     1681 aggacacccc atgacagatt tatcatcttg gacagcctat tttcaggatt ttggacaatt
     1741 tttcaatggt ttcctcttca cccttgccct agcgattgga tcctttatcc tcgccatggt
     1801 cttaggaatc ttctttggtg ccatgtcaac cagtaaacgt ccaattttac gcattttagc
     1861 tcgcatcttt gtggaatttt accaaaacac tcccctcttg gtgcagtttg ttattgtttt
     1921 ttatggccta cctcttatca gtgaccatac tatcatgatt ccgatttatt ggacagctgt
     1981 tctctgcgtg ggactctatc acggcgctta tatcgctgag gttattcgtt cagggattca
     2041 gtctattcct agtggtcaga tggaggccgc cttatcgcaa ggttttacct atatcagtgc
     2101 catgcgcttg attatcttgc ctcagacctt ccgcatcatt ctccctccat tgaccaacca
     2161 aattgttaac ctcatcaaga acacctctac tgtagctatc atctctggag tagacttgat
     2221 gtttgtgact aagtcttggt cggctctcaa cggaaactat atcccagcct ttttaggtgc
     2281 tgctcttctc tactttgccc tatgcttccc tgttgcccag tttggtcgca agatggagca
     2341 agccaacaaa aaagcctatt cactttagga ggttactatg gaatctattt tagaagtttt
     2401 aaccccagat aacctagtct ttatctttaa aggatttggc ttgaccctct atatttctct
     2461 gattgccatc atcctctcta ctatcatcgg tacggtgcta gctgtcatga gaaatggcaa
     2521 aaattctgtc ttgcgaatca tctccagtat ttatatcgag tttgtgcgca acgttcccaa
     2581 ccttctctgg atttttacta tctttttggt gttcaaaatg aaatccacac cagcaggtat
     2641 tacagccttt actctcttta catcagcagc cttggctgag attattcgag gcggtctcaa
     2701 tgccgtagac aagggacaat acgaagcagg aatgtcacaa ggcttcacct cagcccaaat
     2761 cctctactac atcattctcc cacaagccat ccgcaaaatg ctaccagcca tcatttctca
     2821 gtttgttacc gtgattaagg ataccagtct cctctactct gttatcgccc tacaagaact
     2881 ctttggagcc agccaaattc tcatgggccg ttatttcgaa ccagagcagg tcttcagtct
     2941 ttacatcctg attgccctca tctacttcag ctttaaccta gcaatttcta gcctgtctca
     3001 tatgctagcc aaacgttggc aacaagccgc agaataatac tcttcgaaaa tctcttcaaa
     3061 ccacgtcagc ttcaccttgc cgtatatatg ttactgactt cgtcagctct atctgcaacc
     3121 tcaaaacagt gttttgagca acttgcggct agcttcctag tttgctcttt gattttcatt
     3181 gagtataaac agccactctc cagttcaatt ggagagtttt ttgatgagag tagatattta
     3241 tagacctcaa tttgacacca tacattttct atgataaaat gtaacaaatt catttacaac
     3301 aaatcgaaat ctaaatgaaa tggaattttc tatgaaatca gtcaaaggac tactctttat
     3361 catagctagt tttatcttga ctcttttgac ttggatgaac acttctcccc aattcatgat
     3421 tccaggacta gctttaacaa gcctatctct gacttttatc ctagccactc gtctcccact
     3481 actagaaagc tggtttcacg gtttggagaa ggtctacacc gtccacaaat tcacagcctt
     3541 tctctccatc atcctactaa tctttcataa ctttagtatg ggcggtttgt ggggctctcg
     3601 cttagctgct cagtttggca atcttgccat ctatatcttt gccagcatca tccttgtcgc
     3661 ctatttaggc aaatacatcc aatacgaagc ttggcgatgg attcaccgcc tggtttacct
     3721 agcctatatt ttaggactct ttcacatcta catgataatg ggcaatcgtc tccttacatt
     3781 taatcttcta agttttcttg ttggtagcta tgccctttta ggcttactag ctggttttta
     3841 tatcattttt ctatatcaaa agatttcctt cccctatcta ggaaaaatta cccatctcaa
     3901 acgcttaaat cacgatacta gagaaattca aatccatctt agcagacctt tcaactatca
     3961 atcaggacaa tttgcctttc taaagatttt ccaagaaggc tttgaaagtg ctccgcatcc
     4021 cttttctatc tcaggaggtc atggtcaaac tctttacttt actattaaaa attcaggcga
     4081 ccataccaag aatatctatg ataatcttca agccggcagc aaagtaaccc tagacagagc
     4141 ttacggacac atgatcatag aagaaggacg agaaaatcag gtttggattg ctggaggtat
     4201 tgggatcacc cccttcatct cttacatccg tgaacatcct attttagata aacaggttca
     4261 cttctactat agcttccgtg gagatgaaaa tgcagtctac ctagatttac tccgtaacta
     4321 tgctcagaaa aatcctaatt ttgaactcca tctaatcgac agtacgaaag acggctatct
     4381 taattttgaa caaaaagaag tgcccgaaca tgcaaccgtc tatatgtgtg gtcctatttc
     4441 tatgatgaag gcacttgcca aacagattaa gaaacaaaat ccaaaaacag agcttattta
     4501 cgaaggattc aagttcaaat aagtaatcat actctcctgg gatttcccag gagagttttt
     4561 tatagccaaa tgaaataaga caggaacgat ggaattttgg cattcaaatc aattgataag
     4621 aatgttttat agtggagtga aataggatac gaacaaattg attaggaaaa tcaaatgaat
     4681 ttatagaaat cttttagcag ttatgttgtc ctattctagt ttcaaaacgc tatagaagca
     4741 gcattgtact agtctagatt cagtttacta tactaaaacg agtagcttga aatcaaaaaa
     4801 cccaccctca caggcaggtt ttatctgtat tattcagcta gattatgctt taccttctga
     4861 accgaatacg tcgatacgtt cttcaaccga tgcttggata gcttttacac cgtcagccaa
     4921 gaatttacgt gggtcgaaga gttttttctt gtcgtattct gcttcgtttg cttcgtagtc
     4981 acgagcaaat ttacgagttg cgttagcgaa tgcgatttgg cattctgtgt taacgttaac
     5041 tttggcaaca ccaagtttga tagctgcttg gatttgctca tcaggaatac ctgatccacc
     5101 gtgcaatacg attgggaatc ctggaagagc ttctgtcaat ttttgcaagt ggtcaaggtc
     5161 aagaccttcc cagtttactg ggtaagggcc gtggatgtta ccgataccag ctgccaagaa
     5221 gtcgatacca gtttcaacca ttgctttagc gtcttcgatt ggagccaatt cacctttacc
     5281 gatgattccg tcttcttcac caccgatagt accaacttca gcttctactg agataccttt
     5341 agcgtgtgct ttttcaacaa cttctttagc caatttaagg ttttcttcaa ctggaaggtg
     5401 tgaaccgtca aacatgattg aagtataacc aacttcgata cactcaagtg catcttcgta
     5461 gtgaccgtgg tcaaggtgga tagctactgg tacagtgata cccattgatt caacaaggtt
     5521 agcgatcaag ttgcgagcaa ctttgtaacc acccatgtat ttagcagcac ccattgaagt
     5581 ttggatcaaa actggagctt ttttagcttc tgctgcgcgc aagatagctt gagtccactc
     5641 aaggttgttt gtgttaaatc caccaactgc ataaccgttg tcacgggctg cttggacaaa
     5701 tttttctgct gaaacgattg ccattttatc aggcctcctg tatattttta tgggtcatcc
     5761 catttacatt gttcatttta tcactttttg ccaaaaaaat ctagtttttc ccgcagtttc
     5821 gattgatttt cttctaactc catctatgta aaccctttct ctccctagtc ttgggcgact
     5881 tttggaaaag ctataaagaa ggttaaacga ttctcctcca tctcgaaacg ataagctaat
     5941 ttttcatgtt ctaatagact cttaaccaca aagagcccca taccagaccc cttgaccttg
     6001 cgactggcat tgtcagaaaa agactgggct agtttttctt gttcctctga gctacagcta
     6061 ttttcgataa aaagttctcc ttctctttct ccaattcgaa ctaagccacc tggaacagag
     6121 tgcttaatgg cattgctgat gagattagaa agaatcaact tcataactga tgggtttaga
     6181 taagcctgct gatgggtcaa actattgtct atctggagct ctctttcctt ggctagcaag
     6241 gcataatctt tgaccagatt ttgcgtcatc tggaggaggt caattgtttc cctatcatct
     6301 cgcaattcct gcacagaaga gagggaaagt atctgcagaa catggtgatt gagttcatcc
     6361 acaatcccca aggcaactcc cagatactgg tctctatcct tataacgacc gatattctct
     6421 ctcatatttt cgattaggat tttcaaacta gccagcggtg ttttcaattc atgagaagct
     6481 cctcgtagga attcgacctt catcttctcc agctggagaa tggcttcatt cttttcatgc
     6541 aagtccgcaa taacagtcaa gagatgctgg tagaggctat tgatttgttc cttgagatta
     6601 cctatctcat ccttagaatc cacgcgcaat cgcacttggg aatccaggtc catcatccga
     6661 cgggtcaccc gcttgatttc caaaatcggt gcaacaatag tccgggcgta gatgtaggcc
     6721 accaagaggg aaatcagaaa ggaggccagc aaggtatagg gaagaaactg gagactgatt
     6781 tgctccgctt ccttttgtaa atccatggaa gctagaaact ggagaatcat agtaccaccg
     6841 tcttgcgttt tcacctcgcg ctcctcaata aagagagagg ttgtctggcg gtctgtgtcc
     6901 agaggaagac tgtccttgac ttctaacttg tcctcggtca tctcaccttt gacggtcccc
     6961 ttgatatcac tagtctggga atacaagtct aacacttgct cgatactctg cctatctttc
     7021 ccttctaggg actgggcaat ggctgttgcc ttttgaccaa tggtttcctg acgatgactc
     7081 agataagtcg aaggaaaaag aaaataaata gctaaatgaa ggcagataac cagaacacta
     7141 aatatcgaga aggtatagat aaatatcttt gcaaataaac ctgttcgttt cattttcgct
     7201 ccaatttata accaacattg cgcacagtga ggatacaatc caagtctagc tttttccgca
     7261 attccttgat ataaacatca ataacacggt caaagggaac ctcatctgtc gctttccaga
     7321 cggcatcgat aatctgagac cgagtcaagg cccggccttc atttttcact agatagtcca
     7381 gaatttccaa ctctttggca ttgataggca cttcttgacc tgcgaggctt gcactgtagc
     7441 tttcaaagtc caccttggta tccttgtaag aaaagattcg tcctgtatcg tagtagcgct
     7501 tgaaaatcgc gtccaccctc acttttaaga gggagagaga gaaaggtttt tccagatagc
     7561 catctgccaa agaggcaaag gcactcatct tgtattcctc atcctgaaaa gctgtcaaca
     7621 tcaagacagg aacctgactg gttttacgaa tctcagctag gacttctaag ccgttaagct
     7681 tgggcatctg gatatccagt aaaaccaggg ccacctcata gctagaaaat tgctccagag
     7741 cttcctgacc gtccgctgcc tcaatagttt catagccaca atccgtcaaa taatcactga
     7801 ccccctcacg gatcatctct tcatcttcta caattaaaat tttcatactt taactgctct
     7861 ctatttttta tttttcttag aataaatacc tactctattt tctattatag tctcttgctg
     7921 gccttttgtc tgtaagcaac tgatcactag ataaaacgtt gtgaaattcc tttctcataa
     7981 attccataac tttagtatat tatatttaag cactaaagta caaagaaagc aactgaaagc
     8041 aatgattttc accactgctt tcagatttat tttgaattgt taaatagcta ttcctatcca
     8101 ctattcttga atagaaacac aagatgcaat ctttattcca gactcatttt ttcaaattta
     8161 ttcaccatcc agcaagagct cttttggttg ttttctaagg agattgcttg aagcaagcgc
     8221 cataacgaga accactagaa ccaaggcaag gacaaaaatg atgataaagt ctgatgtctg
     8281 aatggaaatg tctaggctcg acaaggtctt gctaaagcca tctacttctg caccgccacc
     8341 aaggttagag gcttgagccg ccttactagc ctgtttggca acacctgaag tcacattggc
     8401 aaggacagtg tttccaattg cacgggcagt gtaattagct aggaagtaag cagaaactag
     8461 agcagggata gcaatcaaga tagattcggt gatgaattga cccaagatac ttgcctgctt
     8521 gaggccgata gagaggagaa ttcccacttc cttgcgacgg gcgttgatcc aaaggctgag
     8581 caagagggca aggaggagaa ctgagaagct caagctaccc cagaagagga ggttggccat
     8641 cttgtacata ccagagatag attgctcaag agctgggtag ttagaggagc tcttgacgag
     8701 tgtgtagctc ttccagttga taccactgat gccattcaac tctttcataa catcatccaa
     8761 gttcttgtct gctgttacaa agaaggttgc gtccccataa atggctgtgt cttctgtgta
     8821 tccataaagt tttgcagcag tgtgaatgtc tgtaatagct gtgttttcgt aaagttcttg
     8881 tgagtaggtt actgctgact tattatgacc atcaaagagt cccttgattg tcacttcaac
     8941 tgtttccttg gctccttttt cattatctgc atcgtagata ttagagtcca gtttaacctt
     9001 gtcccctact ttccagccgt gtttggctgc caagtccttg tgcaagagga ttttatcctt
     9061 gtcgtcgttg gttaagtgct ctccttcgac tagtttataa gaaccagaga caaacttgtc
     9121 ttctttagag gagtcattga cacctgtaat catcaagcta cttccaaaac gtttggcacg
     9181 atcagcagtg agattcttct tggtttctgg cgtttcaatc aggtcatatc cagtcaaatc
     9241 tccgatagcg ttgatacgtt tgacataaga ctcaatggcc ttgttttcgg tgattttttt
     9301 gatgtcttca cccttgatat tcccagcacc gcgaggcgtt ccttggttga cgcgacgatt
     9361 gatttgcatg gagaagctat tggtgatatt tttaaaggtc tcctgagaag ccttggcagt
     9421 agctcccttg attgacaagc cgaccaaact caagctcgcc atgaggagaa taatcaggaa
     9481 gatgacaatc gatttgaaaa acttccttgt aacataggca aatgcgttgt gtaacataga
     9541 ttccctttct agattttgtt ttaatcattc tattaaagta agctcaaatt atttactagt
     9601 attgcgcgtt tcagtcagtt tcttatcctt taattcaagt gtaatatctg acgcttgtgc
     9661 cacttcttta ctgtgagtta cgacaatcac acatttacct gttttctggg caagtgattt
     9721 gagtagttcg acaatatctc cagcagtttt aggatccaga tttcctgttg gctcatcagc
     9781 tagaataact ggagcttctg agaccaaact gcgagcaatg gcaacacgtt gctgttgacc
     9841 acctgataac tggagaacat tccgcttgat ctggctttca tccaaaccaa gctcaagaag
     9901 tgtattcttg cttgcctttt tgttgaccaa tcggatattt tccagcggag aaagataatc
     9961 tatcaagtta taattttgaa agaccaggga aatatggtgc atgcgatggt aagaatagcc
    10021 cttcttacga atatcctctc cttgaaaaag gatagaacct tcaacaggac tatctagacc
    10081 agcaagtagg gacaagagtg tggattttcc tgctcctgac tccccaataa tactgtaaaa
    10141 ttttccgggt tcaaaattat aattgatctg atataggact gcttcagcag tattcttata
    10201 acggtaggta acatcttgta attgtaataa agtcatgatt tctccttctt aactaataga
    10261 tgataaaatt tctttcggtg attttctaaa taagaatagg aaacaaaggg ctacagataa
    10321 gcaactaagc agaactagaa aaacatagga ttctgcaaaa gataagatgc tagttgataa
    10381 actgcttgct ttggctagtg tatcttgtaa gcttgcctga tctccacttg ctagtagagt
    10441 ttggagtagg taagttgtga ttccgtttcc tgcaacaaat gctggaagca aagctccaag
    10501 agataccaaa actacctcta aacagaattg taggaaaatc gagctcttgc cttttccaag
    10561 tgcaagtaaa atccccactt catagacccg ttctctcaac cagagagaca aaaccagaat
    10621 taaggctcca gctcctgcta tcaacatccc ataaaggaag atggtcagga aggtttggaa
    10681 agttgcaact gagtctttga tttgttcaaa agccttgttt tccttttcga cttggtagcc
    10741 ttgattttcc aaggccaagt tttctacctg cttcatgagt ccgtccattt ccttaggatt
    10801 ttctacatag aagcgtgctg cactgacttg agcttcacta ttgcccaaaa gggtttggct
    10861 actttcatag tctgtaaaga cttgattttc actgaagtca gaagacaagc ctgtgaattt
    10921 ctcttgtttt ttaccagaaa agatgccgat aatctcaaac tctactgttt gtccttttcc
    10981 agattcagac tgaccagcat ccaagccaat cttgtcatga agtgaaagac cgttcttctt
    11041 agccaattct tcgtggataa ggattttctt ggaattccct ttttgaaggt gtcgcccttc
    11101 ttttagattg aaagccgaac tggtaaaggt tacatccttg gatgaatcct caagagccgt
    11161 taagctaacc aagttattgt ctgcagctga taaatcatca cgctccacgc tctgctcgcc
    11221 agtcactgct tccttgtctt ttagttttgc gaccgtctcg agttcaggag agacattttc
    11281 cagcccctta atcttgctta cagatgctag gtctgacaac ttgaatgtct gaccattctc
    11341 tatcttctta atagaaaaag atgtattgag tgatttataa agattgcttt ctactgtttt
    11401 gttggacttc atcagagtca aacaggctga aattccggcc aataagacca ataaaatcag
    11461 aaataaaata aaacttctca gtcgctttct gctgacataa gcccaagatc tttggattgg
    11521 attcatttgt cacctccata tttgtaagac tattataaaa cccaaatatg aaatgtttat
    11581 gaaatacgaa aaaaaaatat cgagtagggg ataatctcta gcccctctca
//
LOCUS       DAVLFB010000064        11542 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLFB010000064 DAVLFB010000000
VERSION     DAVLFB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.064"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..141
                     /gene="blpO"
                     /locus_tag="VXD58_001483"
     CDS             <1..141
                     /gene="blpO"
                     /locus_tag="VXD58_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV0676157.1"
                     /translation="KMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGCAT
                     TV"
     gene            245..364
                     /locus_tag="VXD58_001484"
     CDS             245..364
                     /locus_tag="VXD58_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV0676158.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            832..1203
                     /locus_tag="VXD58_001485"
     CDS             832..1203
                     /locus_tag="VXD58_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676159.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            1832..2215
                     /locus_tag="VXD58_001486"
     CDS             1832..2215
                     /locus_tag="VXD58_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV0676160.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2267..2956
                     /locus_tag="VXD58_001487"
     CDS             2267..2956
                     /locus_tag="VXD58_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0676161.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            2998..3231
                     /locus_tag="VXD58_001488"
     CDS             2998..3231
                     /locus_tag="VXD58_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676162.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3382..3993
                     /locus_tag="VXD58_001489"
     CDS             3382..3993
                     /locus_tag="VXD58_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0676163.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4154..4948
                     /locus_tag="VXD58_001490"
     CDS             4154..4948
                     /locus_tag="VXD58_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV0676164.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4945..5580
                     /gene="trmB"
                     /locus_tag="VXD58_001491"
     CDS             4945..5580
                     /gene="trmB"
                     /locus_tag="VXD58_001491"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV0676165.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5706..6185
                     /gene="rimP"
                     /locus_tag="VXD58_001492"
     CDS             5706..6185
                     /gene="rimP"
                     /locus_tag="VXD58_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV0676166.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6229..7365
                     /gene="nusA"
                     /locus_tag="VXD58_001493"
     CDS             6229..7365
                     /gene="nusA"
                     /locus_tag="VXD58_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV0676167.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7387..7680
                     /locus_tag="VXD58_001494"
     CDS             7387..7680
                     /locus_tag="VXD58_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV0676168.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7673..7972
                     /locus_tag="VXD58_001495"
     CDS             7673..7972
                     /locus_tag="VXD58_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV0676169.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            7989..10781
                     /gene="infB"
                     /locus_tag="VXD58_001496"
     CDS             7989..10781
                     /gene="infB"
                     /locus_tag="VXD58_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV0676170.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11032..11382
                     /gene="rbfA"
                     /locus_tag="VXD58_001497"
     CDS             11032..11382
                     /gene="rbfA"
                     /locus_tag="VXD58_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV0676171.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3516 a         2031 c         2587 g         3408 t
ORIGIN      
        1 aaaatgatgt cacaattttc tgttatggat aatgaaatgc ttgcttgcgt tgaaggtgga
       61 gatattgatt ggggaagaaa aattagttgt gcagcagggg ttgcatatgg cgcaattgat
      121 gggtgtgcaa caacggtttg acatttctat tgggaccatt tgctatagga ataggtgtaa
      181 ctggtgctgc aggtggagct atacttggag gtgtagccta tgcagcaaca tgttggtggt
      241 aattatggat tttaaaagtt ttattattgg tttagtagtt ggtatatttg gtccttatat
      301 ggatgactta attagaaaaa tattttcaaa atcttccaaa aaggatacgg atagtactct
      361 ctaaaaaata aatttctccc ctgtatttgc tagcttaatt tacaggggag tttctttaat
      421 attgtttcgc ctgtcaaaat gatatgtgat agcaggatga aaagtctaca aacaagttgg
      481 tatcaataca atataaagag gaaagtatgt ttttcatgct tgctttttta atttttacaa
      541 ttcaagaggt tttgatgacc atttatgatt tgagtgatcc aaggtcaaaa taagtgctat
      601 actagcagtg taaatgttct tgctcaacta agataaatcg cacatttagg aggaaaactt
      661 atgaagaaaa aaatactgat tattttcgtt ctgtatctga tcatgtccat ctttctttat
      721 ccgcttaggg agagtatttg gtataatcta ttttatacca tagcctatat gattgcggtt
      781 atgatctatt tttctttaat taaaaagaaa gaaaagaaat gaaagatttt cttgattaaa
      841 aagaatcata ttctttgtga gattgttcct gggtcagttg cctttgcttg tttctactta
      901 tctatttcta tttcgtcagt tttccaattt cttttagtag ttattaactt gacttctatt
      961 ttggttactg tttatcttac tagggaaatg agattaagag agtttgaaga tgatgatttg
     1021 gttagtccta gaaccaatca actcatgttt atcggcttga caggctttat gtctattatt
     1081 tgtttgtata gaggtatcac agcaggagaa tcttatcaac aactaatcgc ttatattggt
     1141 gctattcttt gcttgatcat catgcttcta ctcatttggg gtttgaagta ttataaaaag
     1201 taggggctaa tgaagttttt gcaatttagg aggtttcgat gacaattcaa gatttggatg
     1261 aaaaatttta aaaaacaatg atatactaaa cttgtcaaag ttgcaacggg tgctgctaca
     1321 ttatgttaaa aatcgctctt gattgataag ttggatttgt taggaaagga gtaaataatt
     1381 atggataaga aaaagatagt ttcgactata atatgtatag tatttcttgt agtgtcagta
     1441 gataattttt ttagagactt aaccccgtta ctgttcattt taaatattat tggcctgtct
     1501 tgcttttcag tgctaacata cattaacata aaagagatac tcttaaatat aagtaaatga
     1561 tgatttggtt agtcctagaa ccaatcaact tatgtttatc ggcttggcag attttatgtt
     1621 tgtcatttgt ttgtatagag gtatttcaga aacagaattc tatcaacaac taatcgctta
     1681 tataggtgta ttctctgctt gtttatcacg cttctgctca tgtggggctt gaagtattat
     1741 aaaaagtagc tttctatcct tttctgacag gatatttgag tagaaggctc tattaaattg
     1801 ttagtgaaat aaatgaatgg aggtatttaa tatgaaatac agattatttt ttgttatttt
     1861 cttgagtagt gtgttggata ttcttttagg gacattttta caaatctcta tcgtatctat
     1921 tgggtggctt gttctttaca gtggtttgtt tgaagcagga gttttccttc ttgctaataa
     1981 aggggtggcg gtaaaaatca aggaagtaga tattcgaaat cgctttaaat ttatttttgg
     2041 aaaaacctta tggtttcaaa ttcttttgct catctttttg ataatcaaac tttatcttgg
     2101 tttggatgcg aggttgattt tattctatgg acatattttc attgtcttta atgccttaat
     2161 gtatctatta tctagtagtc aggttagcct taaaaaaaac aaactgtctt cttaatctta
     2221 cagcagtaga gcctgacaag gtgaggtcat agtagggagg atttctatga aaaagtatca
     2281 acttctattc aaaataagtg cagttttctc ttacttattt ttcgtatttg gtctttctca
     2341 gatgacgctt attattcaaa attattggca attttcttcc cagattggca atttcgtctg
     2401 gattcaaaat ttcttgagtt tgctatttag cggagtcatg atttggattc tggttaagac
     2461 aggtcatggt tatctctttc acattccaag aaaaaaatgg ctttggtatt cgattttgac
     2521 agtattagtg gtagtgctcc agatctcttt taacgttcag acagctaaac atgttcagtc
     2581 aactgctgaa ggttgggctg tattgatcgg ttatagtggg accaactttg ctgagctagg
     2641 tatctatata actttgttct ttctgactcc acttatggaa gagctaatct atagaggatt
     2701 actgcaacac gcctttttta agcattcgag atttggcctt gatttgcttc ttccgtcaat
     2761 tttatttgct cttcctcatt tttcaagcct gcctagtttg ttagatatct tcatctttgc
     2821 aacatctggc atcatctttg ctagtttgac ccgctatacc aagagcattt atccttccta
     2881 tgcggtgcat gtgatcaata atattttcgc aacattacca tttttgctga cttttttaca
     2941 tagggtcttg gggtaaaaaa acaaaaggct tgcttttcag ccatagagga gattatcatg
     3001 tataaacact tatttttcct agattccaaa actttagatc ggttgacacc ctatattcta
     3061 gtcttggctt ctgacaccat tgcttttaat gtttttgtgc taacctttgt atctgcggtg
     3121 gtttttaatt tcctaaattc catgctagct ttaatggcta tattcatagg ggctggctat
     3181 gtggtcggat tttggttact aatactcaat gaaaatcaaa gagcaaacta ggaaactagc
     3241 cgcaggctgt acttgagtac ggcaaggcga cgttgacgtg gtttgaattt gattttcgaa
     3301 gagtataaaa tggtttgttt tggaaaggct agaactaaag aatgacgcgt aggaagtctg
     3361 tttggtgagg aggatagttt tatggagttt tttgataaat ttcatgcctt gtgttttgga
     3421 tttttagtac taataattgt cattacagtt ccttatacga ttaaccatgg gggttttttt
     3481 caaaatgaat ctgcattgat tcttgtaagt cttcttgtaa cctcgctgag tgttgcttat
     3541 gctagaaagt ttgaaatgat ttcttttggg atgttaagca agaaacaact tttgcttttc
     3601 attgcaatct ttcttctaag tgtacttgag acgctggttt atattcattt cttcgctgtt
     3661 tcttctggct caggggtcca acacttggcg gaagtcagca gaggaatttc cctgtctttg
     3721 attttgacta cctcagtttt tggccccatc caggaggaac tcattttcag aggacttctt
     3781 caaggtgcgg tttttgacaa ttcttggtta gggcttgtgc taacttcctc tctcttttct
     3841 ttcatgcatg gaccttctaa tgtcccttcg tttatttttt atctacttgg gggtttgttg
     3901 ctgggctttg cttataaaaa gagtcaaaac ctatgggttt ctactctagt ccacatgttt
     3961 tacaacagtt ggccactcct atattattta taaaaattat gaaaaggtaa gcagaagatg
     4021 gtgcttacct ttttcaagct ataatgatac ccaagccaat ccagaggctt ttctttgaga
     4081 atcgggttag agtggttgac gaataggcca aaaactagta gaatagtaag gaaactttat
     4141 acggaggaaa gaaatggatt tgggtgataa tgagctaaca ctgactccca tacctgggaa
     4201 aagtggcaag gcttatatgg gtagctatcc tgacgggaag cgcatctttg taaaaatgaa
     4261 cacctctcca atcctacctg gtctagctag agaacaaatt gctccacaat tattatggag
     4321 tcgtcgtttg gcagatgggc gtgatatgtg tgctcaagaa tggttgacag gcaagatatt
     4381 gaccccctat gatatgaatc gtaagcaaat cgtcaatatt ttaacccgtc ttcatcgctc
     4441 acgtccgttg atgacacaat tgagtcgttt gggctatgcc atggaaacac ctgtagattt
     4501 actacagtct tggcaggaaa cggctccaga tgctttgcgt aaaaatcatt ttatcagtga
     4561 agtgatggct gatttacgtc agactattcc aggatttaga gaggaccatg cgaccattgt
     4621 ccatggagat gtacgacata gtaattggat tgagacagat agtggcttga tttatttagt
     4681 agattgggat tcggttcgct tgaccgatcg catgtttgat gtggcccata tgctctgcca
     4741 ttatatttca gaacatcagt ggaaggaatg gttgacctac tacggttaca agtacaatca
     4801 aacggtatta agtaaattgt attggtatgg tcaattgtct tatttgagtc agatttccaa
     4861 gtattatatg aaccaagatt tagaaaatgt caatcgggag attcatggtt tgcgtcattt
     4921 ccgagacaag tatggaaaga gaagatgaga gttagaaatc gtaaaggggc aacagaatta
     4981 ctagaggcaa atccccagta tgtggtcctc aatcccttgg aagccaaggc aaaatggcgg
     5041 gacttgtttg gcaatgataa tcccattcat gtggaagttg gaagtggaaa gggtgccttt
     5101 gtttcaggta tggccaagca aaaccctgac atcaactata tcgggattga tattcaaaag
     5161 tctgttttga gctacgcttt ggacaaggtg cttgaagttg gagtgcctaa catcaagctc
     5221 ttgtgggtag atggttctga cttaactgac tattttgaag acggtgagat tgatcgcttg
     5281 tatctgaact tttcagatcc atggccgaaa aaacgccatg aaaagcgtcg tttgacctac
     5341 aagaccttct tggatacctt caaacgtatc ttgcctgaaa atggagaaat tcatttcaag
     5401 acggataacc gtggcttgtt tgagtacagt ttagtgagct tttctcaata tggcatgaaa
     5461 ctcaatggtg tctggttaga tttgcatgcc agtgattttg aaggcaatgt catgacagaa
     5521 tacgagcaaa aattctcaaa caaggggcaa gttatctacc gagttgaggc agaattttaa
     5581 gagatagcct aaaattaggc tgtacaagtg cttttgcttt acataagttg gcaaacgtgc
     5641 tatactgata gtaagaatat gaaaagtgag gcggggaaat atcttcgcct cttgcttatg
     5701 aggaggtgga cgcaatcgca acaatcgtag aattagtcag agaagttgta gaacctgtca
     5761 tagaagctcc ttttgaactc gtggatatcg agtatggaaa gattggcagt gacatgattc
     5821 tcagtatttt tgtagataaa cccgaaggaa ttaccttgaa cgacacggca gacttgacag
     5881 aaattatcag tcctgtccta gacaccatca agccagatcc cttcccagaa caatatttcc
     5941 tagaaattac cagtccaggt ttggaacgcc ctttgaaaac caaggatgcc gtcgctggag
     6001 cggttggaaa atacatccat gtcgggctct accaagccat cgataagcaa aaggtctttg
     6061 aaggaacctt gttggccttc gaagaggacg agttgactat ggaatatatg gacaagacgc
     6121 gtaagaaaac cgtccaaatt ccatacagtt tagtatcaaa agcacgttta gcagttaaat
     6181 tatagaaaaa gaaaggatag cttttgagga ttcaaaagtg aagaaaacat gagtaaagaa
     6241 atgctagagg ccttccgcat tttggaagaa gacaagggaa tcaaaaaaga agatatcatc
     6301 gacgcagtag tagagtcgct tcgttccgct tatcgcagac gctatggtca gtcagacagc
     6361 gtagctattg acttcaacga aaaaacaggt gactttacag tttatactgt ccgtgaagtt
     6421 gttgatgaag tatttgatag ccgtttggaa atcagcttga aagatgctct tgccattaat
     6481 tcagcttatg aacttggaga caaaatcaag tttgaagaag caccagctga gtttggtcgt
     6541 gtagcagccc agtctgccaa acaaaccatc atggaaaaaa tgcgcaagca aacacgtgcc
     6601 atcacttaca atacttacaa agaacatgag caagaaatca tgtctggtac agtagaacgc
     6661 tttgacaacc gctttatcta tgtcaacctt ggtagcatcg aagcccaatt gtcaaaacaa
     6721 gaccaaattc ctggagaagt ttttgcttct catgatcgta tcgaagttta tgtttacaag
     6781 gttgaagaca accctcgtgg tgtgaacgtc tttgttagcc gtagtcatcc agaaatgatc
     6841 aaacgtttaa tggagcaaga aattccagaa gtttatgatg gaactgttga aatcatgagc
     6901 gtggctcgtg aagcaggtga ccgtacgaag gttgctgttc gtagccacaa tccaaacgtg
     6961 gatgctatcg gtacaatcgt tggacgtggt ggtgctaata tcaagaagat tactagcaaa
     7021 ttccacccag ctcgttacga tgctaaaaat gaccgcatgg taccaatcga agaaaatatc
     7081 gatgttatcg agtgggtagc agatccagct gaatttatct acaatgccat cgctcctgct
     7141 gaggttgacc aagttatctt tgatgaaaac gacagcaaac gtgccttggt ggttgttcca
     7201 gataacaagc tttctcttgc cattggtcgt cgtggacaaa acgtgcgctt ggcggctcac
     7261 ttgactggtt accgtatcga tatcaagtct gctagcgaat ttgaagccat ggaagacgct
     7321 gcttcagtag agttggaagt agaaaacgat actgtagaag aataaaagct gctagaggag
     7381 ggaaagatga aaacaagaaa aatccctttg cgcaagtctg ttgtgtctaa cgaagtgatt
     7441 gataagcgtg atttgctccg cattgtcaag aacaaggaag gacaagtctt tattgatcct
     7501 acgggcaagg ccaatggccg cggcgcttat atcaaactag acaatgcaga agccctagag
     7561 gcgaaaaaga agaaggtctt taaccgcagc tttagcatgg aagtggaaga aagcttttat
     7621 gacgagttga tcgcttatgt ggatcacaaa gtgaaaagaa gagagttggg acttgaataa
     7681 gcaaaagata agtaatctct tggggcttgc tcagcgagca gggcgcatca tatcgggtga
     7741 agaattggtg gtcaaggcca ttcaagacgg caaggccaag ttggtctttc tagctcatga
     7801 tgctggaccc aatctgacca agaagattca agataaaagt cattattatc aagtagaaat
     7861 tgtaaccgtg ttttcaacac tggaattaag catagcagtc gggaaatcga gaaaggtttt
     7921 ggctgtaaca gatgctggat ttacaaagaa aatgaggtct cttatggaat agaagaggag
     7981 gacatgattt gtctaagaaa agattgtacg aaatcgcaaa agaacttgga aaagaaagta
     8041 aagaagttgt agcgcgtgca aaagagttgg gcttggatgt gaaaagccac tcatcaagtg
     8101 tggaagaagc tgtcgctgca aaaattgctg ccagctttaa gcctgcagct gctccgaaag
     8161 tagaagcaaa acctgcagca ccaaaagtaa gtgcagaaaa gaaagccgaa aaatctgagc
     8221 cagctaaacc agctgtagct aaggaagagg caaaaccggc tgagccagtt gctccgaaaa
     8281 cagaaaaagt agcagtgaaa ccgcaaagtc gtaatttcaa ggctgagcgt gaagcccgtg
     8341 ctaaagagca ggcagaacga cgcaagcaaa ataagggcaa taaccgtgac caacaacaaa
     8401 acggaaaccg tcagaaaaac gacggccgta atggtggaaa acaaggtcaa agcaaccgcg
     8461 acaatcgtcg ctttaatgac caagctaaga agcagcaagg tcagcaaaaa cgtagaaatg
     8521 agcgccgtca gcaagaggat aaacgttcaa atcaagcggc tccacgtatt gactttaaag
     8581 cccgtgcagc agccctaaaa gcagagcaaa atgcagagta cgctcgttca agtgaggaac
     8641 gcttcaagca gtatcaggct gctaaagaag ccttggctca agctaacaaa cgcaaggaac
     8701 cagaggaaat ctttgaagaa gcggctaagt tagctgaaca agcacagcaa gttcaagcag
     8761 tggttgaagt cgtccctgag aaaaaagaac ctgcagtgga tacacgtcgt aaaaaacaag
     8821 ctcgaccaga caaaaatcgt gacgattatg atcatgaaga agatggtcct agaaaacaac
     8881 aaaagaatcg aagtagtcaa aatcaagtga gaaatcaaaa gaatagtaac tggaataaca
     8941 acaaaaagaa caaaaaaggc aataacaaga acaaccgtaa tcagactcca aaacctgtta
     9001 cggagcgtaa attccatgaa ttgccaacag aatttgaata tacagatggt atgaccgttg
     9061 cggaaatcgc aaaacgtatc aaacgtgaac cagctgaaat tgttaagaaa cttttcatga
     9121 tgggtgtcat ggccacacaa aaccaatcct tggatgggga aacaattgaa ctcctcatgg
     9181 tggattacgg tatcgaagcc aaacaaaagg ttgaagtgga taatgctgac atcgaacgtt
     9241 tctttgtcga agatggttat ctcaatgaag atgaattggt tgagcgtcca ccagttgtta
     9301 ctatcatggg acacgttgac cacggtaaaa caaccctttt ggatactctt cgtaactcac
     9361 gtgttgcgac aggtgaagca ggtggtatta ctcagcatat cggtgcctac caaatcgtgg
     9421 aaaatggtaa gaagattacc ttccttgata caccaggaca cgcggccttt acatcaatgc
     9481 gtgcgcgtgg tgcttctgtt accgatatta cgatcttggt cgtagcggca gatgacgggg
     9541 ttatgcctca gactattgaa gccatcaacc actcaaaagc agctaacgtt ccaatcatcg
     9601 tagctattaa caagattgat aaaccaggtg ctaacccaga acgcgttatc ggtgaattgg
     9661 cagagcatgg tgtgatgtca accgcttggg gtggagattc tgaatttgtt gaaatctcag
     9721 ctaaattcaa ccaaaatatc gaagaattgt tggaaacagt ccttcttgta gctgaaatcc
     9781 aagaactcaa ggcagaccca acagtgcgtg caatcggtac ggttatcgaa gcgcgcttgg
     9841 ataaaggaaa aggtgcggtc gcaacccttc ttgtacaaca aggtaccttg aatgttcaag
     9901 atccaatcgt tgtcggaaat accttcggtc gtgtccgtgc tatgaccaac gaccttggtc
     9961 gtcgtgttaa agttgctgga ccatcaacac cagtctctat cacaggtttg aacgaagcac
    10021 cgatggcggg tgaccacttt gccgtttacg aggatgaaaa atctgcgcgt gcagcaggtg
    10081 aagagcgtgc caaacgtgcc ctcatgaaac aacgtcaagc tacccaacgt gttagccttg
    10141 aaaacctctt tgataccctt aaagctgggg aactcaaatc tgttaatgtt atcatcaagg
    10201 ctgatgtaca aggttctgtt gaagcccttt ctgcctcact tcaaaagatt gacgtggaag
    10261 gtgtcaaagt gactatcgtc cactcagcgg tcggtgctat caacgaatca gacgtgaccc
    10321 ttgccgaagc ttcaaatgcc tttatcgttg gtttcaacgt acgccctaca ccacaagctc
    10381 gtcaacaagc agaagctgac gatgtggaaa tccgtcttca cagcattatc tacaaggtta
    10441 tcgaagagat ggaagaagct atgaaaggga tgcttgatcc agaatttgaa gaaaaagtta
    10501 ttggtgaagc ggttatccgt gaaaccttca aggtgtctaa agtgggaact atcggtggat
    10561 ttatggttat caacggtaag gttgcccgtg actctaaagt ccgtgttatc cgtgatggtg
    10621 tcgttatcta tgatggcgaa ctcgcaagct tgaaacacta caaagacgac gtgaaagaag
    10681 tgacaaacgg tcgtgaaggt ggattgatga tcgacggcta caatgatatt aagatggatg
    10741 atgtgattga ggcgtatgtc atggaagaaa tcaagagata agattttttg ctcctttctt
    10801 aggtggtgag ggacgcaagc aaaccgatgg tttcattgct tatttttgag cctagggtct
    10861 caaaaatccc ctgtgatggg actgataaat cagttccatc actttcacca cggcgaaaga
    10921 agcagatgac ttcaaattga acttcgtttc aatttaaact gaaaatcaag aagtttaaaa
    10981 tagctaggtc tgctggccta gcttttggtt caaagtagag aaaggaatat catggcaaat
    11041 catttccgta cagatcgtgt gggcatggaa atcaagcgtg aagtcaatga gattttgcaa
    11101 aagaaagtcc gtgatccacg tgtccaaggt gtgaccatca cagatgttca gatgctgggt
    11161 gacttgtctg ttgccaaggt ttattacacc attttgagta accttgcttc ggataaccaa
    11221 aaagcccaaa tcgggcttga aaaagcaact ggtaccatca aacgtgaact tggtcgcaat
    11281 ttgaaattgt acaaaatccc agatttgacc ttcgtcaaag acgagtccat cgagtatgga
    11341 aacaagattg acgagatgct acgcaatctg gataagaact aaagaagagg ggttgcccct
    11401 cttttttggt ggaggaaaat aggttgaatt tgaaatggaa aaatattctt ttaaaatata
    11461 ttgaaactag aatagtacgc ctctacttct aaaacattgt tagaaatcga tttgactgtc
    11521 ctgatcgatt tgtcctgttc tt
//
LOCUS       DAVLFB010000065        11525 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLFB010000065 DAVLFB010000000
VERSION     DAVLFB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.065"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(185..946)
                     /locus_tag="VXD58_001498"
     CDS             complement(185..946)
                     /locus_tag="VXD58_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0676172.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(1234..2196)
                     /gene="nrdF"
                     /locus_tag="VXD58_001499"
     CDS             complement(1234..2196)
                     /gene="nrdF"
                     /locus_tag="VXD58_001499"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV0676173.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2384..4543)
                     /gene="nrdE"
                     /locus_tag="VXD58_001500"
     CDS             complement(2384..4543)
                     /gene="nrdE"
                     /locus_tag="VXD58_001500"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV0676174.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4624..4842)
                     /gene="nrdH"
                     /locus_tag="VXD58_001501"
     CDS             complement(4624..4842)
                     /gene="nrdH"
                     /locus_tag="VXD58_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV0676175.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNISYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            5193..5456
                     /locus_tag="VXD58_001502"
     CDS             5193..5456
                     /locus_tag="VXD58_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV0676176.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5462..7195
                     /gene="ptsP"
                     /locus_tag="VXD58_001503"
     CDS             5462..7195
                     /gene="ptsP"
                     /locus_tag="VXD58_001503"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV0676177.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDVFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            7450..9900
                     /gene="phtA"
                     /locus_tag="VXD58_001504"
     CDS             7450..9900
                     /gene="phtA"
                     /locus_tag="VXD58_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV0676178.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            10058..>11525
                     /locus_tag="VXD58_001505"
     CDS             10058..>11525
                     /locus_tag="VXD58_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV0676179.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         3690 a         2147 c         2375 g         3313 t
ORIGIN      
        1 tttaaactag gaagcgagtc gcagatttct caatgcatag ctttgaggaa ttgggcaaaa
       61 agtctttgat atagaaaaaa gcatagtatc aggtgttgaa acacctgata ctatgctttt
      121 tattgtggga agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag
      181 tttattaagg cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta
      241 gtttagaatc tgaaacaata gtatcaagat ttgatatatt ataaaaagta taaaaatcaa
      301 acttattgaa cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga
      361 tgcgttgagc ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat
      421 tacagctaac gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc
      481 caacaaaagc acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt
      541 ttcgttcgct taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa
      601 taggcaactc acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc
      661 tttcttctac tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg
      721 cttgtttttc aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag
      781 ctccaccatg cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg
      841 cagtcatatc tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac
      901 tatttactat ctctaaaatt ttggttaatt tttcttgttt gagcatatta tcacctcgtt
      961 tcctactact atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa
     1021 acaaaaacaa aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttatta
     1081 ttttgttgtt tctagtttac tttttgatgg ttaagagtgg tggagaatta tactcaatga
     1141 aaatcaaaga gcaaactagg aagctagccg caggctgtac ttgagtacgg caaggcgaag
     1201 ctgacgtggt ttgaatttga ttttcgaaga gtattagtcc aaaccgtagt tgtagtcatc
     1261 atcttgcatg gcttcaactt cgccaagaag gtaaccattt ccgacttgag agaagaagtc
     1321 atggttggaa gttcctgttg aaataccgtt cataacgatt gggttgacat cttcagctga
     1381 atctgggaaa agtggatctt gtcccatgtt catgagagct ttattggcat tgtagcgaag
     1441 gaaggtttta acctcttcag tccaaccaac accgtcataa agactctctg tgtagccttc
     1501 ttcattttca taaagagtat agagtaggtc gtacatccat tctttgagtt tttcttgctc
     1561 ttcttcaggt aattcattga aaccaagttg gaatttgtaa ccaatgtagg ttccgtgaac
     1621 agactcgtca cgaataatca atttaatgat ttctgcaacg ttggcaagtt tgttgttacc
     1681 gagatagtag aggggagtga agaaaccaga gtagaagagg aaggtttcga ggaagacgct
     1741 ggcaactttc ttttcaagtg ggctgccgtt taggtagatt tcgttgacaa tctcagcctt
     1801 cttttgtagg taaggattgg tattggtcca ttcgaaaatt tcttcaatct cagccttagt
     1861 attcaaggta gaaaagattg atgagtaaga tttagcgtgg acagattcca taaattggat
     1921 gttattgaaa acagcttcct catgtggtgt acggatgtct gcgcgaaggg cttgaacccc
     1981 agtttcagat tgcatagtgt caagaagggt taaaccacca aaaacttttc cgaccaagtc
     2041 tttctctttg ttagatagct ttctccagtc atccaagtcg tttgataagg gaatacgtgt
     2101 atcgagccaa aattgctccg tcagtttttc ccaagttgat ttgtcgatga catcttcgat
     2161 ggcattccag ttaatggctt tgtagtaagt ttccatttaa aatctctttc tgtgtttagt
     2221 attgcgaact cacaattatt tctactttac cataattcta taggagtatc gcacaaaaag
     2281 tcggaagccc gacttttaaa atgttacata aattatgtta tgacatagta gatttgattt
     2341 tatcagtgct gcttagggaa aaatagtgtt tctatgctag aaactaaatc acacagcttt
     2401 cacattggtt ggcgccgact tctccaccgt catctgtaaa ggtacggacg tagtagatag
     2461 acttgattcc cttgttaaag gcatagttac gaaggatgga caagtcacgt gtcgtttgtt
     2521 tattttccct cttccattcg taaaggcctt ttggaatgtc actacgcatg aagagggtga
     2581 gtgaaagtcc ttgatccacg tgttcagtcg cagcagcgta aacatcgatg actttacgca
     2641 tatccatatc gtaggcagaa gtgtagtaag gaatggtttc tgtagacaag ccagcagcag
     2701 ggtagtagat tttaccaatt ttcttttcct gacgttcttc aatacgttgc gtaatcgggt
     2761 ggatagaagc agaaacatcg ttgatgtagc tgatagaacc atttggcgct acagcaaggc
     2821 gattttggtg gtaaagacca tcctcttgaa ccttgtcgcg aagttcagtc caatcagcaa
     2881 caccagggat aaaaacattt ttgaagagtt ctttaacacg gtctgatgtt ggaacaaatt
     2941 caccagttac atacttgtca aagtaacttc cgttagcata gtctgatttt tcaaagttgt
     3001 ggaaggtaat accacgttca cgcgcaatat tgtttgactc taccaaggtc cagtagttca
     3061 taagcataaa gtagatgctt gtaaattcaa cagactcagg tgaaccatat tcaatgagtt
     3121 gttgggcaag gtagctgtgc agtcccatgg caccgagacc aaaggtgtgg gcttggctat
     3181 ttccatggtc aatcgttggt acagctacga tatgtgaact atctgtaacg aaagtaaggg
     3241 cacgaaccat agcacggata gaacgaccaa aatcaggtga agtcatcatg ttaaccacgt
     3301 tggttgaacc caggttacat gaaacatctg ttcccatttg aaggaattct tgagcatcgt
     3361 tgatcaagct tggttcttga acttgaagaa tctcagaaca caagttactc atgataatct
     3421 ttccatcgac aggatttgca cggttagccg tatctatgtt gactacataa ggatagccag
     3481 actcttgttg caatttagag atttcagttt ccaaatcccg cgccttgatt tttgtcttgc
     3541 gaatatttgg atttgcgacc aattcatcgt atttttcagt aatgtcgatg taattgaatg
     3601 gcacaccgta ttctttttct acagagtaag ggctgaagag gtacatttct tcatttttac
     3661 gagccaattc gtagaattta tcaggtacta caacaccaag tgatagagtc ttgacacgta
     3721 ctttttcatc agcgttttct ttcttagttg aaaggaaagc gatgatatct gggtgaaaga
     3781 cgttgaggta gacaacaccg gcaccttgac gttgccccaa ttggttggag taagagaagc
     3841 tgtcttcaaa aagcttcata acaggaacga cacctgaagc agctccttca tagcctttga
     3901 taggtgcacc agcttcacga aggttgctga gggtaattcc cacaccacca ccaatacgtg
     3961 aaagttgaag agctgagttg atagaacgtc cgatagagtt catatcatcc gtcacttgga
     4021 ttaggaaaca agataccaac tccccacgac gagcacgtcc agcattcaag aaggaaggag
     4081 tagcaggttg gtagcgttgg tggatgattt cattggcaat atcgattgca acagcttcat
     4141 tcccatcagc gaaataaagg gcgttgaaga agacacggtc ttccatattt tcaagatagt
     4201 attcaccgtc attagtcttt aaggcatatt gattgtaaaa tttataagct gccatgaatg
     4261 acttgaattg gaagttttgg tctttgataa attgagccaa ttcttccaag aactctggac
     4321 ggtatttctt gataaaggct gtttcgatgt agttgtgttc aatgaggtaa ttgattttgt
     4381 ctttgattga atcaaaaacc atagtgtttg gaactacatt ttctttaaag aaagcatcca
     4441 aggcttcctt atctttatga agcatgattt gtccattaac aggacggtta atttcgttat
     4501 taagacggaa gtaagtcacg tcttcaagat gttttaatcc cataaaattt cccttatcta
     4561 attacaaaag aaaggcttct aagttagccc taaaagcagt ttcttctgga tgatgtacta
     4621 agattatgct aattgtttca gtttttctgg ttggaaacct gaaaagactt cagttggtgt
     4681 ttggataaca ggagctgcgc taaaaccgag ctctttaact tgatcgacgt actcaggttg
     4741 ctcatcaaga ttgatttcac gataagagat attattactg tccaagaaac gcttggtcat
     4801 tttacattgg acacaattgt ttttagaata aacggttacc attgtgtaac tcctcttcaa
     4861 aatttaatac tatcttagta tatcagaaaa taaaattttg tcaatgattt aaactaaata
     4921 tagtggtgtt cttttgttgt ttttatatat aaacacaata tatagtgttt gtattgtgaa
     4981 atagcaatga ttatcctata aattgttttt gaagaaacta tatcctgtgt tttgttatct
     5041 aggatagaag attttcagca agttatctga aaggaaattg aaggaaaatc cagaaaatgc
     5101 ttgaaaaaaa tcctagaaga tggtataata ctaaattgta agggttatca catataactc
     5161 aaaaaaagaa agaacaaaag gagagtcaaa ctatggcttc taaagatttc cacgtagtgg
     5221 cagaaacagg tattcacgca cgtccagcaa cattgttggt acaaactgct agcaaatttg
     5281 cttcagatat cactcttgag tacaaaggta aatcagttaa ccttaaatca attatgggtg
     5341 ttatgagtct tggtgttggt caaggtgctg acgtaactat ctcagctgaa ggtgcagatg
     5401 cagatgacgc tatcgctgca atctcagaaa caatggaaaa agaaggattg gcataaggga
     5461 aatgacagaa atgcttaaag gaatcgcagc atctgacggt gttgcagttg caaaagcata
     5521 tctactcgtt cagccggatt tgtcatttga gactattaca gtcgaagata caaacgcaga
     5581 agaagctcgc cttgatgccg ctctacaggc atcacaagac gagctttctg ttattcgcga
     5641 gaaagcagta ggtacgctcg gtgaagaagc agctcaagtt tttgatgctc acttaatggt
     5701 tcttgctgac ccagaaatga tcagccaaat caaggaaact atccgtgcga agaaagtgaa
     5761 tgcagaagca ggtctgaaag aagttacaga tgtgtttatc actatctttg aaggcatgga
     5821 agacaaccca tacatgcaag aacgcgcagc ggatatccgc gacgtgacaa aacgtgtatt
     5881 ggcaaacctt cttggtaaaa aattgccaaa cccagcttct atcaatgaag aagtgattgt
     5941 gattgcgcat gacttgactc cttcagatac agctcaattg gacaaaaact ttgtaaaagc
     6001 ttttgtaacc aacattggtg gacgtacaag ccactcagct atcatggcac gtacacttga
     6061 aattgctgct gtattaggta caaacaacat cactgaaatc gttaaagacg gtgacatcct
     6121 tgctgttaac gggatcactg gagaagtgat tatcaacccg acagatgaac aagcggcaga
     6181 atttaaagca gctggtgaag cctatgcgaa acaaaaagct gaatgggcac ttttgaaaga
     6241 tgctcaaaca gtgactgctg acggtaaaca cttcgagttg gctgccaata ttggtactcc
     6301 aaaagacgtt gaaggtgtta acaacaacgg tgcagaagct gttggacttt accgtacaga
     6361 gttcttgtac atggattctc aagacttccc aactgaagat gagcagtatg aagcatacaa
     6421 ggctgttctt gaaggaatga acggtaaacc tgttgtcgtt cgtacaatgg atatcggtgg
     6481 agataaggaa cttccttact tcgatatgcc tcacgaaatg aacccattcc ttggattccg
     6541 tgctcttcgt atctctatct ctgagactgg agatgctatg ttccgcacac aaatccgtgc
     6601 tcttcttcgt gcgtctgttc acggtcaatt gcgtatcatg ttcccaatgg ttgcgctctt
     6661 gaaagaattc cgtgcagcga aagcagtctt tgatgaagaa aaagcaaacc ttcttgctga
     6721 aggtgttgca gttgcggata acatccaagt tggtatcatg atcgagattc ctgcagcggc
     6781 tatgcttgca gaccaatttg ctaaagaagt tgacttcttc tcaattggta caaacgactt
     6841 gatccaatat acaatggcag cagaccgtat gaacgaacaa gtttcatacc tttaccaacc
     6901 atacaaccca tcaatcctac gcttgattaa caatgtgatc aaagcagctc acgctgaagg
     6961 taaatgggct ggtatgtgtg gtgagatggc tggtgaccaa caagctgttc cacttcttgt
     7021 cggaatgggc ttggatgagt tctctatgtc agcaacatct gtacttcgta cacgcagctt
     7081 gatgaagaaa ctcgacacag ctaagatgga agagtacgca aaccgtgccc ttacagaatg
     7141 ctcaacaatg gaagaagttc ttgaacttca aaaagaatac gttaattttg attaatcgaa
     7201 aagtccctgc aactcagtta cagggacttt ttttgatatt ttaaaaagaa ttttcaagaa
     7261 aatctttctt ataaaaagtc caaccttgaa aaagtagtgg tcagaacaaa aaatacttaa
     7321 atggttcata aaattcttga caagttggag atttaggagt aaactattaa ccagttaagt
     7381 aatagagagg agtttctgca atttagaaat gaattgcaac tagaaatatc aaatagaaag
     7441 agagtttcga tgaaaattaa taagaaatac cttgttggtt ctgcggcagc tttgatttta
     7501 agtgtttgtt cttacgagtt gggactgtat caagctagaa cggttaagga aaataatcgt
     7561 gtttcctata tagatggaaa acaagcgacg caaaaaacgg agaatttgac tcctgatgag
     7621 gttagcaagc gtgaaggaat caatgctgag caaatcgtca tcaagataac agaccaaggc
     7681 tatgtcactt cacatggcga ccactatcat tattacaatg gtaaggttcc ttatgatgcg
     7741 attttcagtg aagaattact catgaaagat ccaaactata agctaaaaga tgaggatatt
     7801 gttaatgagg tcaagggtgg atatgttatc aaggtagatg gaaaatacta tgtttacctt
     7861 aaggatgctg cccacgcgga taacgtccgt acaaaagagg aaatcaatcg acaaaaacaa
     7921 gaacatagtc aacatcgtga aggtgggact ccaagaaacg atggtgctgt tgccttggca
     7981 cgttcgcaag gacgctatac tacagatgat ggttatatct ttaatgcttc tgatatcata
     8041 gaggatactg gtgatgctta tatcgttcct catggagatc attaccatta cattcctaag
     8101 aatgagttat cagctagcga gttggctgct gcagaagcct tcctatctgg tcggggaaat
     8161 ctgtcaaatt caagaaccta tcgccgacaa aatagcgata acacttcaag aacaaactgg
     8221 gtaccttctg taagcaatcc aggaactaca aatactaaca caagcaacaa cagcaacact
     8281 aacagtcaag caagtcaaag taatgacatt gatagtctct tgaaacagct ctacaaactg
     8341 cctttgagtc aacgacatgt agaatctgat ggccttgttt tcgacccagc gcaaatcaca
     8401 agtcgaacag ctagaggtgt tgcagtgcca cacggagatc attaccactt catcccttac
     8461 tctcaaatgt ctgaattgga agaacgaatc gctcgtatta ttccccttcg ttatcgttca
     8521 aaccattggg taccagattc aaggccagaa caaccaagtc cacaaccgac tccggaacct
     8581 agtccaggcc cgcaacctgc accaaatctt aaaatagact caaattcttc tttggttagt
     8641 cagctggtac gaaaagttgg ggaaggatat gtattcgaag aaaagggcat ctctcgttat
     8701 gtctttgcga aagatttacc atctgaaact gttaaaaatc ttgaaagtaa gttatcaaaa
     8761 caagagagtg tttcacacac tttaactgct aaaaaagaaa atgttgctcc tcgtgaccaa
     8821 gaattttatg ataaagcata taatctgtta actgaggctc ataaagcctt gtttgaaaat
     8881 aagggtcgta attctgattt ccaagcctta gacaaattat tagaacgctt gaatgatgaa
     8941 tcgactaata aagaaaaatt ggtagatgat ttattggcat tcctagcacc aattacccat
     9001 ccagagcgac ttggcaagcc aaattctcaa attgagtata ctgaagacga agttcgtatt
     9061 gctcaattag ctgataagta tacaacgtca gatggttaca tttttgatga acatgatata
     9121 atcagtgatg aaggagatgc atatgtaacg cctcatatgg gccatagtca ctggattgga
     9181 aaagatagcc tttctgataa ggaaaaagtt gcagctcaag cctatactaa agaaaaaggt
     9241 atcctacctc catctccaga cgcagatgtt aaagcaaatc caactggaga tagtgcagca
     9301 gctatttaca atcgtgtgaa aggggaaaaa cgaattccac tcgttcgact tccatatatg
     9361 gttgagcata cagttgaggt taaaaacggt aatttgatta ttcctcataa ggatcattac
     9421 cataatatta aatttgcttg gtttgatgat cacacataca aagctccaaa tggctatacc
     9481 ttggaagatt tgtttgcgac gattaagtac tacgtagaac accctgacga acgtccacat
     9541 tctaatgatg gatggggcaa tgccagtgag catgtgttag gcaagaaaga ccacagtgaa
     9601 gatccaaata agaacttcaa agcggatgaa gagccagtag aggaaacacc tgctgagcca
     9661 gaagtccctc aagtagagac tgaaaaagta gaagcccaac tcaaagaagc agaagttttg
     9721 cttgcgaaag taacggattc tagtctgaaa gccaatgcaa cagaaactct agctggttta
     9781 cgaaataatt tgactcttca aattatggat aacaatagta tcatggcaga agcagaaaaa
     9841 ttacttgcgt tgttaaaagg aagtaatcct tcatctgtaa gtaaggaaaa aataaactaa
     9901 tgaaaaatga aagtctcgat aaagaggctt tcatttttat tatgtatata tgtaaaattc
     9961 ttgacaagca atattaaaaa gagtaaacta ttaactagtt aattaaccgg tttattactt
    10021 tatagtgaat caaatatact taagaaaaga ggaaagaatg aaaatcaata aaaaatatct
    10081 agctgggtca gtagctacac ttgttttaag tgtctgtgct tatgaactag gtttgcatca
    10141 agctcaaact gtaaaagaaa ataatcgtgt ttcctatata gatggaaaac aagcgacgca
    10201 aaaaacggag aatttgactc ctgatgaggt tagcaagcgt gaaggaatca atgccgaaca
    10261 aatcgtcatc aagataacag accaaggcta tgtcacttca catggggacc actatcatta
    10321 ttacaatggt aaggttcctt atgacgctat catcagtgaa gaattgctga tgaaggatcc
    10381 aaactaccag ctaaaggacg aggatattat cagtgaaatc aagggtggtt atgtgattaa
    10441 ggtagatgga aaatactatg tttaccttaa ggatgcagct catgcggata atgtccgtac
    10501 aaaagaagaa atcaatcggc aaaaacaaga acatagtcag catcgtgaag gagggacttc
    10561 aacaaacgat ggtgcggtag cctttgcacg ttcacaagga cgctatacta cagatgatgg
    10621 ttatatcttt aatgcttctg atatcattga ggacacgggt gatgcttata tcgttcctca
    10681 cggcgaccat taccattaca ttcctaagaa tgagttatca gctagcgagt tagctgctgc
    10741 agaagccttc ctatctggtc ggggaaatct gtcaaattca agaacctatc gccgacaaaa
    10801 tagcgataac acttcaagaa caaactggct accttctgta agcaatccag gaactacaaa
    10861 tactaacaca agcaacaaca gcaacactaa cagtcaagca agtcaaagta atgacattga
    10921 tagtctcttg aaacagctct acaaactgcc tttgagtcaa cgccatgtag aatctgatgg
    10981 ccttgttttc gacccagcgc aaatcacaag tcgaaccgcc agaggtgtag ctgtccctca
    11041 tggtaaccat taccacttta tcccttatga acaaatgtct gaattggaaa aacgaattgc
    11101 tcgtattatt ccccttcgtt atcgttcaaa ccattgggta ccagattcaa gaccagaaca
    11161 accaagtcca caatcgactc cggaacctag tccaagtctg caacctgcac caaatcctca
    11221 accagctcca agcaatccaa ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg
    11281 cgatggttat gtctttgagg agaatggagt ttctcgttat atcccagcca aggatctttc
    11341 agcagaaaca gcagcaggca ttgatagcaa actggccaag caggaaagtt tatcgcataa
    11401 gctaggagct aagaaaactg acctcccatc tagtgataga gaattttaca ataagactta
    11461 tgacttacta gcaagaattc accaagattt acttgataat aaaggtcgac aagttgattt
    11521 tgagg
//
LOCUS       DAVLFB010000066        11177 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLFB010000066 DAVLFB010000000
VERSION     DAVLFB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.066"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..168)
                     /locus_tag="VXD58_001506"
     CDS             complement(<1..168)
                     /locus_tag="VXD58_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676180.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVR"
     gene            complement(176..655)
                     /locus_tag="VXD58_001507"
     CDS             complement(176..655)
                     /locus_tag="VXD58_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0676181.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(666..1364)
                     /locus_tag="VXD58_001508"
     CDS             complement(666..1364)
                     /locus_tag="VXD58_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV0676182.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(1357..1623)
                     /locus_tag="VXD58_001509"
     CDS             complement(1357..1623)
                     /locus_tag="VXD58_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV0676183.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(1689..2615)
                     /locus_tag="VXD58_001510"
     CDS             complement(1689..2615)
                     /locus_tag="VXD58_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV0676184.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(2690..3505)
                     /locus_tag="VXD58_001511"
     CDS             complement(2690..3505)
                     /locus_tag="VXD58_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676185.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGVFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(3509..3829)
                     /locus_tag="VXD58_001512"
     CDS             complement(3509..3829)
                     /locus_tag="VXD58_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV0676186.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(4036..4116)
                     /locus_tag="VXD58_001513"
                     /pseudo
     CDS             complement(4036..4116)
                     /locus_tag="VXD58_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4160..6157)
                     /gene="metG"
                     /locus_tag="VXD58_001514"
     CDS             complement(4160..6157)
                     /gene="metG"
                     /locus_tag="VXD58_001514"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV0676187.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(6281..7540)
                     /locus_tag="VXD58_001515"
     CDS             complement(6281..7540)
                     /locus_tag="VXD58_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676188.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(7542..8243)
                     /locus_tag="VXD58_001516"
     CDS             complement(7542..8243)
                     /locus_tag="VXD58_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676189.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(8227..9426)
                     /locus_tag="VXD58_001517"
     CDS             complement(8227..9426)
                     /locus_tag="VXD58_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV0676190.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            9691..11037
                     /gene="gor"
                     /locus_tag="VXD58_001518"
     CDS             9691..11037
                     /gene="gor"
                     /locus_tag="VXD58_001518"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV0676191.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         3305 a         2545 c         1960 g         3367 t
ORIGIN      
        1 tctaacaaaa tcttcccaaa atctggaatc caactcccct gctaggtata gaaaattccc
       61 aacatcataa aagcttgttg tcctatcatt gtttcggtag acagttccaa tttgagattt
      121 aagaccatag agaaccatat ttttcttcca tgttttaaat agttccatta tccctctact
      181 tcaccttcca attcatgtaa gaagtcttgt ccagttctgg caatcctaat aattcagact
      241 taaccttcaa gactaatggc gatgcatttt cttctgtaat ctcttgaata tccatccaaa
      301 tatatccaag tgaatcattc gcaccatcag acacagcttc cgaaatcgta acttgaggtg
      361 cactctcatt catttcaaca tcatacaagg ctatgacatg gtgaaccata aaatttttta
      421 actcttccct gacgaaaaca tcgtagattc gaggattaga gtagcttcta acagtaaatc
      481 ccgtctcttc cataacttct ctagtcagcg tttccgtcag tccttcacca agttgctgac
      541 tgcctccagg tagatcatac cgatgttgat aagggcctct cgttttttca atgcaaagta
      601 actttccatt ttcaaagcaa acacagtaga ccccaaagtg atttttgatt tccatccaac
      661 tcctcctact tcaaagacca gccaccatct attgtcagaa tttgtccctg catggcagat
      721 gcctttccac tggccaaaaa gagactgact tctgccactt cctctggttc aatccagcgc
      781 ttgattgggg tttcactagc cacccagtca gctaagccac ctgtctcaaa atccgcagcg
      841 gtcatagctg tcttgactgc tcctggagcg ataccaaaga cctgaatcct agcttcagca
      901 tagtctagag ccaactgctt ggtgaagcca gccaaggcat gcttggatga agtataggcg
      961 tgaccacctc cacctgctag gctagaagca atggaacaca tattgatgat gattcccttt
     1021 ttattttcca gcatttgtgt caaataatac cgagtcaact ctactggaat aatgtagttg
     1081 atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca acagtggttt gtaagcatcc
     1141 aaaactccgg cagtattaca caaaacatcc acctgagggc accagtcaaa aataggttcc
     1201 aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac cctctaagag tggcttttca
     1261 ccttggtcaa ctccataaac ttgatagccc ttctctaaaa agaggcgagc ttgagccaat
     1321 ccgatccctg aactcactcc tgtaatgagt acacgtttag tcatgcactt ctacccaatc
     1381 cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa aaaccttctc cttctccaga
     1441 aacgttgatt aggaaataag gtgtcatttc aagtgcaagc ccattttgct cgatggtatc
     1501 aaagagttgg acatagtttt ccgcacctcc ccaaccagtt cgtacatatt ttctcttagc
     1561 ctttaaccca ggcaggatct cttcaaatgt catgtttttc tcctttaatt ctacattctt
     1621 catttaatta tagcaaaaaa ccactttata cggctttttg actgtgagtt attcaaacct
     1681 gctactactt acggcaaatt attccctgca gcaagataaa tttcatacca ttcttttctt
     1741 gttaagctaa agtttgccgc tcggctaact tctctcaagt gcttaggatt tgttgtacct
     1801 acgactgcct gcatttttgc tggataacgc aatatccaag aaatggcaat agttgaagag
     1861 gttactccat atttaatagc taaacgatca agtacttgat ttaaagcttg aaatttctca
     1921 tttccaacaa agttcccttt aaaatacccg aattgcaaga cagaccatgc ttgaatgacc
     1981 acatcgtgta attggcaata ttcaaaaatg ctgccatctc gcatagctgc ttgactatct
     2041 tccatattaa catgaaaagc tgattcaaat cctggagtaa aagccgcact caattgtagc
     2101 tgattaacag ctaacggctg cttgacatct tttttaagca actccatcat cataggattt
     2161 tgattagaaa ctccaaaatc tcgaacttta ccttgtttat aaaggagatc aaaggcttct
     2221 gctacttggt cagattccat caaagcatct ggtcgatgaa ggagcaagct atctagatga
     2281 tcaatcttca atctttgcaa aataccgtct actgatttta taatatagtc cttagaaaaa
     2341 tcaaaatagg taaattcttc aatgcgaatg ccacatttgg actgaatcca catcttttct
     2401 cttaaatctg gacgattttt taggacaaga cctaacagtt cttcacaacg accacgacca
     2461 taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa gtgctgtttc tacaagctct
     2521 tcaacttctt ttacagattt atcttttatt ctcatcattc cgagaacaat ttctgataat
     2581 tctttgtcat cttgaccaag agttatgtat ctcatcaaat ttttctcctt taatttctaa
     2641 cattcttccc ttcattataa caaaaaaccg ctttgcaacg gctttttgac tatacttcac
     2701 tccattttat cttcttaaac ccacggaaca agacaaagat tccaataaag aggacagcta
     2761 aaggaataac ttttgtaagg aaaacatttg aaattcccat ccactcataa taacggagca
     2821 gagaacccac cacaagatgg gcaataatca tactgacaaa tggacgaaag accgcttctt
     2881 tccaattcca aataccgata actagcgaaa tcgtaaagac agacaaacta tcccagggag
     2941 ccggaatata aaagactcct tcttgtatga agcttgccat tcctacatat cctaaaacaa
     3001 ctagaagaac tatagtccca acaacaatgt aagtgccaat tttcatttta ggagaatctt
     3061 ggactaaact tcttcgtaaa attgtggcca caagtccaaa tccaatcaga aaaataagaa
     3121 gttgccctaa aaatgtgagc aaattgactg ttaatagagg acctttagaa aaatcactta
     3181 gtagttgata ataacgtaat accgccagga caagaattgg cgtcaaaagg gactctttga
     3241 tagaactgcg aggtgctccc ttgagaatct ctttcattat ttttttagga ttcttaccta
     3301 gataatcctc tgcactcatg ccatctcgtt ctgcttctga gaaatctagc atcatcaaat
     3361 agatctgctc tctgagatag tcttcatcat agagaaatcc agcaagatta aaactttccc
     3421 acaactcctc aaaatacttt tgattctcct cagaaaactc atgtagcaaa gcgcttgttt
     3481 cttcgtaata cttcattttc ttcatggttt aacccccatt cttaatccct tctacttttt
     3541 gactcaaatc gtcccattgt tgccaaaaga ctgagacacg ctcttctcct tctttcatta
     3601 atgaaaaata cttccgatct ggaccatctg gcgacgggcg catgtcgcct cttatccatt
     3661 gatttttttc taacttttgc aacaaaggat aaatagttcc tggaacgata gtatcaaatc
     3721 cagcctctcg caaagtctga accaactcat aaccataccg ctctttttga ccaatcatat
     3781 ccaagacaca accttcaaga acacctttta atagctgagt ttctttcatc acttttccct
     3841 tctaatctat tttgtaatac ctactagtaa cttcacctat agtatatcac ttctacacta
     3901 gtttgtaaag cataatagtt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
     3961 cctaccttat gtatggttac tgacttcgtc agtttcatct acaacctcaa aactgtgttt
     4021 tgagctgact tcgtcagttt catctacaac ctcaaaactg tgttttgagc aacctgcggc
     4081 tagcttccta gtttgctctt tgattttcat tgagtataaa taaaaaaaca gaactagcct
     4141 gaactagtcc tgtctacttt tacccaatca cacttccatt tggtacagct ggatcaactg
     4201 tgagaagggt taatttgcca tcatgttcag ctgagagaat cataccctgg ctgacatatt
     4261 ttttcatcat tttacgtggt ttgaggttag caatgatttg aactttcttg ccgaccaatt
     4321 cttgttcatt tggatagtat tttgcaattc ctgaaagaat ctgacgatct tctccatcac
     4381 cagcatccaa gcggaattga agcaacttat ctgaaccttc tactttagac acttctttga
     4441 cttctgcgac acggatttca accttgtcaa agtcttcaaa cttgatttca tccttgttta
     4501 gtttgagctc aacttcgtcc ggattccatt ctttttcgac tgctggttta ttgccttcca
     4561 tttgttcctt gatataggcg atttcttctt ccatatttag acgtggaaag ataggtgttc
     4621 ctttggcaac tacagtcaca tctgctggga agtcagccaa actcaagttt tcaagactag
     4681 aaacttcttc caaaccaagt tgagtcaaaa ctgcacgact agtttccatc ataaatggtt
     4741 caatcaagtg agcaactaca cgaatgctgg ctgccaagtg gctcatgaca cttgccaatt
     4801 ggtcacgaag agcttcatcc ttggccaaga cccatggtgc agtctcatcg atgtatttat
     4861 tggtacgaga gatcagagtc cagactgctt caagcgcacg tggatagtca actgcttcca
     4921 tgtgtgtatg gaagtctgcg attgattgtt ctgcaacctc agcaagaaca tgatcaaatt
     4981 cagtcacacc ttctacatag gcagggattt gtccatcaaa gtacttatta atcatggaaa
     5041 ccgtacggtt aaggaggttc ccaaggtcat tagccaattc atagttgata cggccgacat
     5101 agtcttcagg agtaaaggtt ccgtctgaac caactggaag gttacgcatg aggtagtaac
     5161 gaagtggatc tagtccataa cgctctacca acatttcagg gtaaacgaca ttcccttttg
     5221 acttagacat ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa cgatcaggta
     5281 atttaacatc caacatcata agaaggattg gccagtagat agagtggaag cgaaggatat
     5341 cttttcctac catatggaag actgttccat tccagaactt gtcaaagtta ccatgttcgt
     5401 cttgagcgta gccaagagct gtcgcatagt taagaagggc atcaatccaa acgtagacaa
     5461 cgtgttttgg atttgatggg acaggcactc cccatgtaaa ggttgtacga gataccgcca
     5521 aatcttccaa acctggctcg atgaagttgc gtagcatttc attaaggcga ccatctggcg
     5581 tgataaattc aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat ttgctaaggc
     5641 gaaggaagta tgattcttca gaaacccatt caacctcatg acctgatgga gcaataccac
     5701 cagtcacatt tccagcttca tcacggaaaa cttctgccag ctggctttct gtaaagaatt
     5761 cttcgtctga tactgaatac caaccagagt attcacccaa gtagatatca tcttgagcaa
     5821 gtaagcgttc aaagacttgt gcgacaactt tttcatggta gtcatcagtt gtacggataa
     5881 atttatcgta tgagatatct agtaattgcc agagttcttt aactccaacc gccattccat
     5941 caacataggc ttgaggtgta ataccagctt cttccgcttt ctgctgaatt ttctgaccat
     6001 gttcatcaag acctgtcaga taaaatacat cgtagcccat caggcgtttg taacgtgcta
     6061 ggacatcaca tgcgatagtt gtgtaggcag aaccgatatg aagtttccca gatggatagt
     6121 aaatcggcgt tgtaatataa aaattttttt cagacataat ttttcctttc caggcaaatg
     6181 aaacctgttt ttctaacact tcattatatc acatttttaa tgaatttcga tagggaaatc
     6241 catacaaaaa caagatagac gagtgtccat cttgttgatc tcattcataa cgaagggctt
     6301 caattggatc aagtttcgat gccttgttgg ctggcaagac tccaaaaatc ataccaacac
     6361 tagccgaaac tgcaagacta aatagggcaa ctgggattga tactccaact tctatacctt
     6421 ctattaaacc ttgcagtaac aaacctgcta aggcagttaa accacttgca attgtcaagc
     6481 caattaagcc acctaacaag gtcaaaatca tggattcaat caaaaactga attaaaatat
     6541 tggcacgtgt tgcgcccaaa gccttacgaa gaccaatctc acgagtgcgc tctgtcaccg
     6601 aaaccagcat gatgttcatg acaccagttc ctccaacaaa gagagaaatc cctgcgatgg
     6661 aactaataat cgtcgtcata aaactaaacg attgttgaat ttctgcaaat acaacggact
     6721 catctgccac ctggtattct ccctgttgta agcctgcaag ctctgtcatt tttcgtgcca
     6781 gttctggacc cagagttggg gttaaactgg tatcattcac tcgaaagaca atactagcta
     6841 tttcatctat attaaaattc gcagcaaggg agatattggt agtaataggc aagccaccaa
     6901 acccatatat ttttgacctt ttagcctccg gactagtata aaccccaatg acccggtaac
     6961 taaatccatt gacttctaca accttgttaa tagcctcttg aggagattca aataaactaa
     7021 tggacaattc ctcatctagc aaaataacac ttgcaaactc tttgaaatct tgctctctca
     7081 gactacgacc tgcaataatt tcattcttaa cagcgtccat gtaagttctg tttccacctg
     7141 tcaaattagc attctcaacc tttttatctt gataggtcaa gatggcattc gttgaattgg
     7201 ttacatagta actatccact cccttcagtt tagctgcctc ttggacccag gattcttgcg
     7261 gttttggcgg ttcaacagga atttcctctt cctttccaga aaccgtaaaa gatgattgtt
     7321 tctgagtaaa agaaccatct ttactttttt taggagagaa aaagacgcta atatttttct
     7381 gagatttagt catatcttta ttgacttgac gagataggga atcacccaaa gccataatca
     7441 caacaactga tgaaacaccg ataataatcc caatcatagt aagcaaagaa cgcatcttgt
     7501 gagccatgat agatgaaaag gcaaatttca gattctgcat cttagttttc ctcctttcct
     7561 aactgagcac tgtcagacga aatgacccca tcccgaatga caatctgacg tttagcatag
     7621 gcagcaatct caggctcatg cgttaccatg ataatggttt ttccttcttt attcaaatca
     7681 accaataatt gcataatttg gttacctgtt ttggtatcca aggctcctgt cggttcatcc
     7741 gctaggataa tagaaggatt gtttaccaag gcacgcgcaa tggctacacg ttgcttttga
     7801 ccaccagata attctgaagg taaatggtga ctacgttctg tcaattcaac cttgtctaaa
     7861 tattcctcag ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat caagggcaat
     7921 tctacatttt gcagagcatt gagcttcgat agaagaaaga actgctgaaa gacaaaaccg
     7981 atttgttggt tacggacctt agccagttgt ttttcaccaa gcccagccac ttcttgacct
     8041 tcaagataat attctccact ggttggtgta tccaacatgc caatcgtatt catcagagtg
     8101 gacttaccag acccagatgg tcccatgatg gctacaaatt caccctcatt cacttctaga
     8161 ttgatatttt tgagaacctg cagttcttgg tcaccattac ggtagcttct gaagatattt
     8221 tttagactaa ttagttgctt catcagcctt cacctctttt ccttcttcca aggaagatgt
     8281 tggattactg atgaccttag caccattcgt taaaccagaa gtgatttctt gattttctgc
     8341 gtcagcattt cccaatgaaa cctcaacttt tttagccttt tgttgttcat ccacaatcca
     8401 gacataattt ttactatcat ccattactag actgctaaca ggaacaagaa tagccttagt
     8461 tttgctttta acctcaatgt tgacagaaaa accctgtttc aaatcaccaa cctcgcctgt
     8521 cacatcaata gtataagggt atttagaacc tgtattattc ccggctgctg gactagctgc
     8581 ttcaccattg tttttaggat agtcagaaat atagcttaat ttcccagtcc attttttatc
     8641 aggatacact ttagaagtaa agcttacttc ttgacctaca gaaaggttgg ctagattgta
     8701 ctcagacaat tctcccttga cttgtaaatt ttcattgctg acaatatgaa ccataacttg
     8761 gctcgcccct gttggagatt tagaaacatt gctattgact tcgactacag ttccctctag
     8821 ggtactgaga acagttgttg catccaattg actttgagcc ttgcttaatt gcgctgcagc
     8881 atctgcacgc gcatcacggg catcacccaa ttgagcgtca atagaagcaa cagaatttcc
     8941 agccactgga gttgggcttt gcaccgttgc atcttctcct cctactggcg ctggtaactg
     9001 tggagccgga gctgaagcgg cttcatttcg tgcttgattg agttcattga tatgacgatc
     9061 tgccttagct actgctcgac tagctgaatc ataggccgcc tgcgcttctg aactactgta
     9121 cttgactaaa gcctgccctt cgctgacctt atcgcccaca gaaacaagga tttcatctaa
     9181 atcaccctta ctagcatcaa aataaacata ttgttcattt tttgctgtta ctgtccctga
     9241 caataaaaca gaggaggcca cgcttccttc cttggcaaca acaagatgag taggctcatc
     9301 ttttagagca gtctgagaag gttgtctaaa gagtaaaatc cccccagcac ccaatacaac
     9361 tacactcgca gcaccgattg ctgcatacag ttgccacttt ttagctttac cattcttttt
     9421 cttcataatg aaactccttt tcttttttac aatactttgc tattatacca aatttccctc
     9481 cagcaaacaa tacagttcag gattaaacaa tcgttcggaa ttttgctttt ctattgtact
     9541 agatatataa tacactatct tgacttattt ggcaagcttt tttgcaattt tttgtgcgta
     9601 gcagattttt gcaatcaaat caaaaaaaga ataaaatata actatcaaaa aagacgctct
     9661 actatttaaa agattacaaa ggagttttca atgagagaat atgatatcat tgctatcggt
     9721 ggaggtagcg gaggaatcgc taccatgaac cgtgctggtg aacatggagc caaagcagcc
     9781 gttattgagg aaaagaaatt aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa
     9841 atcatgtggt acggggcgca aatcgctgag actttccatc aatttggaga agactacggc
     9901 tttaagacta ctgatcttaa ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac
     9961 attgatcgcg ctcgttcttc ttatgatggt agttttaaac gcaacggtgt agacttgatt
    10021 gaaggtcatg ctgaatttgt agattctcat actgtaagcg taaatggtga actgattcgt
    10081 gctaaacata tcgtgattgc tacaggtgcc catccaagta ttcctaatat tcctggtgct
    10141 gagctaggtg gctcttctga tgatgtattt gcctgggaag aacttccaga gtcaattgcc
    10201 attctaggcg ctggttatat cgccgttgaa ttagctggcg tactccacac ttttggtgtc
    10261 aagacagatc tctttgttcg ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt
    10321 gaaggtttgg tcaaggaaat ggaaagaaca aacttaccac ttcacactca caaagtccct
    10381 gtcaagttag aaaaaactac tgacggcatt accattcatt tcgaagatgg tactagtcac
    10441 acagctagcc aagttatctg ggctacaggt cgccgtccaa acgttaaggg cttgcaactt
    10501 gaaaaagctg gagtgactct gaacgaacgt ggctttatcc aagtggatga ataccaaaat
    10561 actgttgttg agggaatcta tgctctaggt gatgtaacgg gcgagaaaga actgactcca
    10621 gttgcaatca aggccggacg taccctatct gaacgtctct ttaacggaaa aactactgca
    10681 aaaatggatt actcaactat tccaactgtt gtcttttcac accctgctat cggaactgtt
    10741 ggtttgacag aagagcaagc tattaaagaa tacggtcaag accaaatcaa ggtttataaa
    10801 tcaagctttg catctatgta ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa
    10861 ctcataacag ctggttcaga agaaaaagtt gtcggacttc atggaattgg ctacggcgtt
    10921 gatgaaatga ttcaaggatt tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt
    10981 gatgcaactg tggcaattca cccaactgca tctgaagaat ttgtaaccat gcgttaatcg
    11041 aaacaaaaga agcaaagcaa aaaacacttc taaatatcaa aaagcgagta tttccgtagt
    11101 tggagatact cgttttctta tgttttattt tatagtatgt tgaagtaaga tatgcaaaaa
    11161 tcaattaaca aaccata
//
LOCUS       DAVLFB010000067        11019 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLFB010000067 DAVLFB010000000
VERSION     DAVLFB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.067"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            2..358
                     /locus_tag="VXD58_001519"
     CDS             2..358
                     /locus_tag="VXD58_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676192.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGWVGGILVII
                     GGSLFLATLKKFKSEE"
     gene            552..842
                     /locus_tag="VXD58_001520"
     CDS             552..842
                     /locus_tag="VXD58_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676193.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            893..1585
                     /locus_tag="VXD58_001521"
     CDS             893..1585
                     /locus_tag="VXD58_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV0676194.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1638..4049
                     /locus_tag="VXD58_001522"
     CDS             1638..4049
                     /locus_tag="VXD58_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV0676195.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAVRASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4299..5000
                     /locus_tag="VXD58_001523"
     CDS             4299..5000
                     /locus_tag="VXD58_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676196.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5002..7710
                     /locus_tag="VXD58_001524"
     CDS             5002..7710
                     /locus_tag="VXD58_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0676197.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGIVVGETQIQFYWTYSLLALVLSWLASVLPVYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSETPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7806..8297)
                     /gene="tpx"
                     /locus_tag="VXD58_001525"
     CDS             complement(7806..8297)
                     /gene="tpx"
                     /locus_tag="VXD58_001525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV0676198.1"
                     /translation="MVTFLGNPVSFTGKQLQGGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8420..9349)
                     /gene="psaA"
                     /locus_tag="VXD58_001526"
     CDS             complement(8420..9349)
                     /gene="psaA"
                     /locus_tag="VXD58_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV0676199.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9381..10229)
                     /locus_tag="VXD58_001527"
     CDS             complement(9381..10229)
                     /locus_tag="VXD58_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV0676200.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10226..10948)
                     /locus_tag="VXD58_001528"
     CDS             complement(10226..10948)
                     /locus_tag="VXD58_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV0676201.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3211 a         2058 c         2527 g         3223 t
ORIGIN      
        1 aattagtggt atcgtcggtc ttgtgggagg aattttactt cttattggtc cttttgtctt
       61 gttgggaaca gcggtaaaca cagttgctac aactcttaat ggaggagcta ctgcaggggc
      121 tttttcaggt gttgccttac tcttgaatgc cttgaagatt gcaaatcttg ttcttggtat
      181 cattgctatt gtttactata aaggagataa tcgtgtaggt gcagctccgt ctgtactaat
      241 gattgttttt ggtggagtta gtctcattcc attcttagga tgggttgggg ggattcttgt
      301 tattatcgga ggatctctat tccttgcaac attgaagaaa ttcaaatcag aagaataaaa
      361 ggtattttag catgaaaaga acaaaaaagt ttatcggtat aggagtagct ctattatctc
      421 tttttcttct agttgcatgt ggaacataaa gttcaaagga tacttcaaca agtaatgatg
      481 agaagacagt agcaacatcc aatagttcaa aagaaacaat cactttcgat acaccggttg
      541 taacagacga tgtgattgaa tcaatacgca cttatgcaga ttatatagat ctttataaaa
      601 atatttttga tgattatttt actaaagctg aggaaggttt caaaggcaca gctatggaaa
      661 ataatgactc gtttactaaa ctaaaagagt caactcaaaa attattcgat gcgcagaaaa
      721 aaaggttaaa taatgaagat agaatagaaa caactaaaaa caatgtgatt gccaaacatt
      781 gtcaaacagt cctttccttt ttggttttga ctagcttttt tgtgaaaaat tgtgtaaaat
      841 agaatagata aacgagggga aacctcggaa aatttaaagg agaatccatc taatggtaaa
      901 attggttttt gctcgccacg gtgagtctga atggaacaaa gctaaccttt tcactggttg
      961 ggctgatgtt gatttgtctg aaaaaggtac acaacaagcg attgacgctg gtaaattgat
     1021 caaagaagct ggtatcaaat ttgaccaagc ttacacttca gtattgaaac gtgctatcaa
     1081 aacaactaac ttggctcttg aagcttctga ccaattgtgg gttccagttg aaaaatcatg
     1141 gcgcttgaac gaacgtcact acggtggttt gactggtaaa aataaagctg aagctgctga
     1201 acaatttggt gatgagcaag ttcacatctg gcgtcgttca tacgatgtat tgcctccaaa
     1261 tatggaccgt gatgatgagc actcagctca cacagaccgt cgttatgctt cacttgacga
     1321 ctcagtaatc ccagatgctg aaaacttgaa agtgactttg gaacgtgctc ttccattctg
     1381 ggaagataaa atcgctccag ctcttaaaga tggtaaaaac gtattcgtag gagctcacgg
     1441 taactcaatc cgtgcccttg taaaacacat caaaggtttg tcagatgacg agatcatgga
     1501 cgtggaaatc cctaacttcc caccattggt attcgaattc gacgaaaaat tgaacgtcgt
     1561 ttctgaatac taccttggaa aataaaaaat tgtaagtcta gaattgattt ctaggctttt
     1621 tatgttagta tggaagtatg ataaggaata aaaaacaaga ttatgtactg gcctacaagc
     1681 aaccagtttc aaccacttac atgggttggg aagaagaagc tttaccgata ggcaatggtt
     1741 ctttaggagc aaaagtattt ggccttatag gggctgaacg gattcaattt aatgaaaaaa
     1801 gtctctggtc tggaggtcca cttcctgata gttcagatta tcagggtgga aatcttcagg
     1861 atcagtatgt ttttttagct gagattcggc aggctttgga gaagagagat tacaatctgg
     1921 ctaaggaact ggctgagcag cacctaattg ggccaaaaac gagtcaatat gggacctatc
     1981 tgtcttttgg ggatattcac attgagttca gccagcaagg tacgactttg tctcaggtga
     2041 cggactatca gagacagctg aatattagta aggcacttgt gacgacttct tatgtctata
     2101 agggaacgcg atttgaacgt gaagcttttg cgagttttcc agatgatctc ttggttcagc
     2161 gttttactaa ggaaggggcg gaaactctag attttactat agaactatcc ttgacctgtg
     2221 atttggcttc tgatggaaag tatgagcagg aaaaatctga ttacaaggag tgtaagttgg
     2281 atattactga ttctcatatc ttgatgaagg gaagagttaa ggataatgat ctgcggtttg
     2341 ctagttatct agcttgggaa acggatggag atattagagt ttggtcagat agggttcaga
     2401 tatcaggagc cagttatgcc aatctcttct tggccgctaa gacggatttt gcccaaaatc
     2461 ctgctagcaa ttatcgcaag aaactagatt tagagcaaca ggtgatagac ttggtggaca
     2521 cagctaaaga aaagggctat acccaattga aatcaaggca tatcgaggac taccaagcct
     2581 tattccagcg tgttcaattg gatttggaag ctaatgttga cgcattcact acagatgatt
     2641 tgttaaaaaa ttataagcca caagaagggc aggctttgga ggagctgttc ttccagtatg
     2701 gacggtattt attgattagt tcgtccagag actgcccaga tgctctacca gctaacctac
     2761 agggagtctg gaatgcggtc gacaatcctc cttggaattc ggactatcac ttaaatgtca
     2821 atctgcagat gaattattgg ccagcctatg ttaccaatct cctagagacg gtctttccag
     2881 tcatcaacta tgtagatgat ttgcgtgtct atggtcgtct agcggctgta aagtatgcag
     2941 gaatcgtctc tcagaaaggt gaggagaatg gttggttggt tcatactcaa gcgactccct
     3001 ttggttggac ggcacctggt tgggattact attggggttg gtcaccagct gccaatgcgt
     3061 ggatgatgca aaccgtttat gaagcctatt cattttatag ggaccaagac tatctcaggg
     3121 agaaaattta tcccatgttg agggaaacgg ttcgtttttg gaatgccttt ttacataagg
     3181 atcagcaggc gcagcgttgg gtgtcttctc cgtcttattc cccagaacat gggccgattt
     3241 cgattggcaa tacttatgac caatctctga tttggcagtt atttcatgat tttattcagg
     3301 ctgctcagga attgggactg gatgaggact tgttgactga ggttaaggag aagtctgatt
     3361 tactaaatcc tttgcaaatc actcaatctg gtcgaatcag ggagtggtat gaggaggaag
     3421 agcagtattt tcaaaatgag aaagtggagg cccagcatcg gcacgcttcc catctagtgg
     3481 gactctatcc tggcaatctc tttagctaca agggacaaga gtatattgaa gcggtacgtg
     3541 ctagcctcaa tgatcgtgga gatggcggca caggctggtc caaggctaat aagatcaatc
     3601 tctgggcgcg tttgggagat ggcaatcgag cccataaatt attggcagag cagttaaaga
     3661 catccacctt gccaaatctt tggtgtagcc atcctccttt tcagatagat ggtaattttg
     3721 gtgctactag tggcatggca gaaatgttac tccagtctca tgcagcttat ctggtacctc
     3781 tagctgccct acctgatgct tggtcaacag gttctgtttc aggcttaatg gcacgtggac
     3841 attttgaagt gagcatgagc tgggaagata aaaaactctt acagttgacc attttatcaa
     3901 ggagtggagg agatttgcga gtttcttatc cagatattga gaagagtgtg attaaaatga
     3961 atcaagaaaa aataaaagcg aaatgcatgg ggaaagattg tatttcggtg gcaacagcag
     4021 aaggtgatct tgttcaattt tatttttaag aagatgttat aaggcagtaa tttgaaactg
     4081 ccttttaata aggatttaag aatataagca gttttcaact agttgaaaaa acgttataat
     4141 gataatagga agtaatactc aatgaaaatc aaagagcaca aactaggaag ctagccgcag
     4201 gttgctcaaa acagtgtttt gaggttgcag atggaagctg acgtggtttg aagagagatt
     4261 ttcgaggagt ataatttgtt tgatagaggg tgggtctgat ggcttatatt gagatgaaac
     4321 actgttacaa gcgttatcag gttggggaca cggagattgt ggccaattgt gatgtgaatt
     4381 ttgagattga aaagggggag ctggttatta tccttggtgc ttcaggtgca ggcaagtcaa
     4441 cagttcttaa ccttcttggg ggaatggata ccaatgatga aggggaaatc tggattgatg
     4501 gtgttaatat tgcggattat agttcccacc agcgcaccaa ttaccgtaga aatgatgtgg
     4561 ggtttgtttt tcagttttat aatctagttt ctaatctgac agctaaggaa aatgtggaac
     4621 tggcttctga aattgtgaca gatgccttga atcctgatca ggtcttgaca gatgtaggtc
     4681 tggctcatcg tctcaataac tttccagccc agctttctgg aggggagcaa cagcgagtct
     4741 ccattgcacg cgcggtagcc aaaaatccta aaattctcct ttgtgatgaa ccgactggag
     4801 ccttggatta tcagacgggc aagcaggttt tgaaaattct ccaagacatg tctcgtcaaa
     4861 agggagcgac ggtgatcatc gtgactcata atggagcttt ggcgcccatt gctgatcgcg
     4921 tgattcaaat gcacgatgcc agtgtcaagg atgtggtgct caaccagcat cctcaggata
     4981 ttgacagttt ggagtactag catgatcaag cgaaaaactt attggaagga cttaattcag
     5041 tccttcacag gctccaaggg gcgtttttta tccatcttga tcctgatgat gttgggatct
     5101 ctagccttag taggcctcaa agtaaccagt cccaacatgg aggcgacagc taatgcttat
     5161 ttaacaactg ctcaaacctt ggatttggca gtcatgtcta actatggctt ggatcaagca
     5221 gaccaagaag aactaaaaca gacggagggc gcagaggtcg agtttggcta tttgacagat
     5281 gtgactatgg ataatgggca ggatgccatt cggctgtact ccaaaccaga gcgaatttca
     5341 acctttcagc taagaaaggg acgacttcct cagtcagaca aggaaatcgc tttggccact
     5401 catttgcaag gccaatacag cgtgggacag gagattagtt ttaaagaaaa agaagagggt
     5461 cattcctctt taaaagacca tacttatacc attactggtt ttgtggattc ggctgaaatc
     5521 ctctcccagc gagatatggg ctacgcagga agtggaagtg ggactctgac agcctatggg
     5581 gtgattttac ctagtcagtt tgatcagaaa gtctacaata tagctcgttt gaaatatcaa
     5641 gatttagcag gtttaaatgc cttttcatca gcttatgaag aaaaatccaa gcaacatcag
     5701 gaagaccttg aacaaacttt atcagataat ggcaaggtac gtctgcaact tttgaaaaaa
     5761 gaaggacaag agtctctaga caaggggcaa gagacccttg acaaggctca gactaatttg
     5821 caggaaggca agcgtcgttt agcagctgct caagctcgta tacaggctca agaaagtcaa
     5881 ctagccttgt ttcctcaagt tcagagagag caggctagtg ctcaacttac ccaagccaag
     5941 caggaattgg gcaaggaaga ggacaaacta aagcaagctg aacaaaatct agcccaagaa
     6001 aaggaaaaat tagaaaaaca tcagcaagtc ttggacgatt tggcggagcc aaggtatcag
     6061 gtctataatc gtcagaccat gccaggtggt cagggctatc ttatgtatag caatgcttca
     6121 tccagtattc gagcagtggg caatatcttt cctgtggtac tttatgccgt agcatccatg
     6181 gtgaccttta cgaccatgac tcgctttgta gacgaagagc gaactcatgc agggattttt
     6241 aaggccttgg gttaccgtag taaggatatt atcaccaagt ttctccttta tggactagta
     6301 gctgggactg tcggaacggc tctaggtagt atacttggtc attatttgct agccagtgta
     6361 atttcaagtg tcattacaaa aggcatagtg gtgggagaaa cccagattca gttctattgg
     6421 acctatagct tactagctct tgtcttgagt tggttggcga gtgtgttacc agtctatctg
     6481 gtggcttgga gggaacttca tgccgaagca gcccagcttc tacttcctaa acctcctgtc
     6541 aaaggagcta aaatcttatt ggagcgtatc ggttttatct ggcgtcgtct cagttttact
     6601 cataaggtaa cagcccgcaa catctttcgt tataagcaga gaatgttgat gacaatcttt
     6661 ggtgtggcag gttctgtagc tctgctcttt gcaggtttgg gaatccaatc ttctgtagca
     6721 ggagttccgt ctaaacagtt tcaacaaatc caacagtatc agatgcttgt ctctgaaact
     6781 cctagtgcga ccaatcagga caaggtagag ctagcagaag tgttgaaagg gcaggagata
     6841 ctagcctacc agaaaatcta ttctaaaacg ctagacaagg atttcaaagg caaggctggt
     6901 cttcaaaaca ttactcttat gatgatagag aaggaagatt tgactccctt tatccatctt
     6961 caacatcatc agcaggagct gacattaaaa gatggcatcg ttattacagc taaactcgcc
     7021 cagctggcag gtgtcaaggt tgggcagact ttagaaattg aaggtaagga actaaaggtc
     7081 gctgctatta ctgagaacta cgttggtcac tttatttata tgagtcaggc tagctatgag
     7141 caactttacg gacagctacc ccaagccaac acttatctgg tctcattaag ggataccagt
     7201 gcaactagta tcgaaagtca ggcgggcttg cttatgaatc aatctgcggt gtccagcgtt
     7261 gtccaaaatg cttcagccat tcgactcttc gactctatcg ctagctcact caatcagacc
     7321 atgaccatct tggtcatcgt atcggttcta ttagctattg tcatccttta caatctgacc
     7381 aatatcaacg tagctgagag aatccgtgaa ctctccacta tcaaggttct cggttttcat
     7441 aataatgaag tcaccctcta catttaccgt gagacgattg tgctgtccct tgtgggaatc
     7501 gtacttggtc tggtagctgg tttctattta caccaatttt tgattcaaat gatttcgcct
     7561 gcgactattc tcttttatcc gcaggtaggc tgggaagtct atgtaatccc agtggcagca
     7621 gtaagcatca ttttgacctt gcttggtttc ttcgtcaatt attatctgag aaaggttgat
     7681 atgttagaag ccctgaaatc tgtagagtaa ggtagttatt tttagctgat tgaacttcta
     7741 tttattaata ttcaaaaatc ctccgtttca aagagcaggg aactctttgt gacagaggat
     7801 tttttctata gggctttagc agctgcaatt gcggcttcga agtttggctc agaattgata
     7861 ttatccacgt attcaacgta gcgaatcgta ttgtcagtat cgaggacaaa gactgcgcgt
     7921 gctaataggt gccattcgtt gatcaagagg gcataatcgc gcccgaaaga atggtcaaag
     7981 tagtctgaaa gcataatggc attgtcaagg ccttcagcac cgcaccaacg tttttgagca
     8041 aaaggtaggt ccattgaaac agtcaatacg accgtgttgt ccagtccagc taattcttca
     8101 ttaaaacgac gtgtttgagt tgagcagatg cctgtatcga tagaaggaac gacactcagg
     8161 acttttttct tgccatcaaa atcagccaga gattttttag aaagatctgt tgtagtaaga
     8221 gaaaaatcaa gcgccttgtc gccgccttgt agttgtttac ctgtaaagct cacaggattt
     8281 ccgagaaaag ttaccatagg atactccaat cttttttctt ccattgtatc tgaaacagtc
     8341 agaattttcc aatgatttga ccggaaatgt gggcatagaa aaaacgccag ctcacatgag
     8401 aatgacgttt ttcagaggct tattttgcca atccttcagc aatcttgtca aggttgtatt
     8461 tcatcatgct gtagtagctg tcgccttctt taccttgttc tgcgatagag tcagtaaaga
     8521 tttgtgcgta gattgggatg tttgtgtctt gagaaacagt tttcattgga cggtcatcca
     8581 cacttgattc tacaaagagt gatggaactt ttgtttggcg aagtttttca accaaggtct
     8641 tgatttgttc aggagttcct tcttcttcag tattgatttc ccagatgtag gcacttggga
     8701 caccataggc tttagagaag tatttgaatg ctccttcgct ggttacaatg agtttctttt
     8761 cagcagggat cttattaaat ttatccttac tttctttatc aagtttgtct aacttatcag
     8821 tatattcttt gagatttttt tcatagaatt ctttattgtt agggtctttg gcgctcaatt
     8881 gtttggcgat atttttagca aaaataatac cgttttcaag gttaagccaa gcgtgtgggt
     8941 cttcttttcc tttttcattt tgaccttcaa ggtagataac atcaacgccg tcgctgactg
     9001 cgaagtagtc tttgttttca gttttcttgg cattttctac caattttgta aaccaagcat
     9061 tgccacctgt ttcaaggttg ataccgttat agaaaatcaa atcagcctca gaagttttct
     9121 taacgtcttc aggaagtggt tcgtattcgt gtgggtcttg cccaatcgga acgatactat
     9181 gaaggtcaat tttgtcacca gcaatatttt tagtaatatc agcgatgatt gagtttgtag
     9241 caacaacttt tagtttttga ccagaagttg tatctttttt tccgctagca catgctacaa
     9301 gaatgattgc agaaagaaag agaacgagta atgtacctaa ttttttcatt agatcctcca
     9361 atttattagg gctttgcccc ttattttaac aaatgtttat ttttcagttt caaatatcgt
     9421 tgtttgggag cgataaagaa gctaatgaga aagaaactag cagctgtaag cacgatacta
     9481 gaacctgccg caacattaaa actatagcca ataaagagtc ccaaaactga agcagtagct
     9541 ccgaaggttg aggaaaggaa aatcatgctt ttcagactat tagcatacag ataagcagtt
     9601 gcagctgggg taatcagcat ggctacaatc aggatagttc cgacactttg catggctgtc
     9661 acagacacga gagtcaggag taccatgaga aggtagtgat agaaattgac aggcattccc
     9721 atggctttag ccaagagttc atcaaaggaa gttatcaaga gttgcttgaa gaaaatccag
     9781 attaacaaga ggatggctgc ccccacaccc atagtaataa acatatccgt atcttggacg
     9841 gccaggatat taccaaaaag gatatggaaa aggtcagttg aacttttagc gacaccaatc
     9901 aagatgatac cgagggctaa gaaagaagaa aaggtaatgc cgatggcggt atcgcttttg
     9961 ataatcgagt ttcctttgat gtaggtaatg atgatggcag ctagcaatcc aaagacaatg
    10021 gctccgataa agaagtcaag gcccaagatg aaggagaggg ctacacctgg taagacagca
    10081 tgtgaaatgg catctcccat gagtgacatc ccgcgtagaa taatgaaaca tcccacagct
    10141 ccagctacaa tcccgacgac aatagctgtt atcaaggcat tttgtaggaa atggaatttt
    10201 tgcaatccat cgataaattc tgcaatcata ggtcacctcc attgaaaaag agttgattac
    10261 cgtaagcttc ttttagattg gtttcggtaa aagtttcttt tgttggacca aaggcaatca
    10321 cttctcgatt gacaagtaag acttgatcga agtagtgggg aatcttgctg aggtcgtggt
    10381 gaacgatgag aaccgtcttc ccagcttttt tcaaatctct cagcgtattc atgatgattt
    10441 cctcactgac agagtcaatc ccagcaaagg gttcatccaa gaggatatag tcggcttcct
    10501 gcaccaaaca tctggcaatc aagacccgct ggaattgacc tccagacagt tgactaattt
    10561 gacgttcagc gtagtcagct aggccgacga tttcaagggc ctcttgcact ttcttccaat
    10621 gtttagcctt taaacttcga aagagaggaa tagagggaaa tagtcctaac gagacgcatt
    10681 ccttgacctt gatgggaaag ttgtagtcga tattgatttt ttgttcgaca taggcaattc
    10741 ggtgtaagga ttttttaact tccttgtcat cgagaaatgc ctgaccttga tgtgggataa
    10801 ttcccagcat accttttaat agtgttgatt tcccagcgcc gtttggacca atgatgccgg
    10861 taattgttgg tccatggagc actagtgaaa tatccttaag tgccaacgtt tctttgtagg
    10921 agacactgag gttttcgata cgtatcataa acttgtattc ctcctgtctc ttaatataca
    10981 ttaaaaaaaa aattaagtca agttaatttt tgaaaaaat
//
LOCUS       DAVLFB010000068        10893 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLFB010000068 DAVLFB010000000
VERSION     DAVLFB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.068"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(18..953)
                     /locus_tag="VXD58_001529"
     CDS             complement(18..953)
                     /locus_tag="VXD58_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV0676202.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(950..1810)
                     /locus_tag="VXD58_001530"
     CDS             complement(950..1810)
                     /locus_tag="VXD58_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0676203.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRAGQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1918..4080
                     /locus_tag="VXD58_001531"
     CDS             1918..4080
                     /locus_tag="VXD58_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV0676204.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4172..5431
                     /locus_tag="VXD58_001532"
     CDS             4172..5431
                     /locus_tag="VXD58_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0676205.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5445..6311
                     /locus_tag="VXD58_001533"
     CDS             5445..6311
                     /locus_tag="VXD58_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0676206.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSISMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6325..7158
                     /locus_tag="VXD58_001534"
     CDS             6325..7158
                     /locus_tag="VXD58_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0676207.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7159..7239
                     /locus_tag="VXD58_001535"
                     /pseudo
     CDS             7159..7239
                     /locus_tag="VXD58_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7516..8958
                     /gene="gtfA"
                     /locus_tag="VXD58_001536"
     CDS             7516..8958
                     /gene="gtfA"
                     /locus_tag="VXD58_001536"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV0676208.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9096..10289
                     /locus_tag="VXD58_001537"
     CDS             9096..10289
                     /locus_tag="VXD58_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV0676209.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
BASE COUNT         3191 a         1893 c         2300 g         3509 t
ORIGIN      
        1 attataattg tgttatttta cttccaacta ttgagagaaa tttcgccact atttagccag
       61 atttcttttc cgttatcaca ttgaactaaa agttttccat tttctgagat gtctttagca
      121 agtcccttgt agtctttttg ctgtagtgtg aaagtgactt cttttcctag aatgaatgac
      181 tgttttttgt ataggtataa tagctcttct gctggtgttt cgaagaaagc acgccagatt
      241 tctatgatca attcattcct tgttatagga gctgtagctt taaataagct ggcagctttt
      301 tcttttaatt cctgagggaa gtctttaata gtgaaattga tacctactcc aataatgata
      361 tctgtgacta agccagtttc tacagaggtc attgcttcag taaggattcc tccaatttta
      421 tgattgttta gatagatatc attgacccat tttatgtcga catctattaa agttaggttc
      481 ttaatagctt tgtagacagc tccagctaca agtagtgtgt aggatggtaa tttgtcatag
      541 gggagatttg gtttaagatg gagtgtcata taaataccac cttgtggtga gtagaaggaa
      601 cgttgaaaac gtcctcggcc tgctgtttga taggaagcta gatagagggt atttgcttca
      661 tggcctaaat caattgcttc ttttgcatct agttgtgttg attttgtttc gggtttaaag
      721 ctgactttaa ttggaagatt ttcttctaga atctctggaa gaataaggtc accattcatt
      781 agtttatatc ctctattttt gatactatca atttcaatgc cttcttgttc tagtcgcttg
      841 atagctttcc aaattgatgt tcggcttagg gatagttttt ctgcgatttt ttctccgctg
      901 atatagtcgg tttctttaga taggatttgg tagacagctt ggtaggattt cataatgttg
      961 cctttctcac tagttggtat tgagagtatt cttttcttgt atgacttgga gtctgattaa
     1021 agtattgttt ataagctttc gaaaaatgga gtggatctga aaaacctacc gagtatgcaa
     1081 ttaccttgat ggactcttgg gtattttcga gaagttgtcc agctcggtgc attcgaacgt
     1141 agagtaggta ttctttgggt gataaggtat taaattcttt gaatacgctt gataagtagc
     1201 ttctgtgaac ggatagttct tttgctaaat cttgaattgt aagtgattga ggatagtggc
     1261 tatcaattaa tcgtttgcat tcaagataga gttggtgggt tgatgaaata ttcttttttt
     1321 tctgattggg agcaatagtt cccagatgaa acatcagttc atgaagttgt cccatgatat
     1381 ggagttgagc taattcactt gattttgtaa tctgagcgaa gcggacaatg tctgagatga
     1441 gttttgcagt agtctgggta tgacaagttt cagattggat gagataggat tgatcagaaa
     1501 tttgggaaag agcaaaataa tcaggggctt tccctccagt gattcctaac cagtagtagg
     1561 cccaaggttc tttactatct gcttgataaa aggttagttc ctctggtttt aatagaaaga
     1621 aatctccttc ttttaaatca acaattttac ccttgtaatg aaattttcct tgtcctttag
     1681 taatgtaatg tagaacgtat gtatcacgaa tggctggacc aaaagagtaa ttaggtgtgc
     1741 attcctcata tccataaaag cttagggcaa ggtcgattgt tccagtctgg tattctgaaa
     1801 aaactaacat gtgctacctc ctacctaaca ttttaccata ttcttgagac atttttctat
     1861 tttggaaagc gatttcaggt gataaaatat aatcaataaa gtgatgaggt gaagtaaatg
     1921 ggagttagga tagagaataa tctattttat gttgagagta aaaatctaag tttgattatt
     1981 gaaaatcgaa atggctactt acttttgaaa catttaggaa agactattaa gaactataaa
     2041 ggttccaata gtgtttatga acgagaccat gccttttcag gaaatccaac ggctactaat
     2101 cgaaccttta gtttagatac tcaacgtcaa atttttggac aacatggctt aggagatttt
     2161 aggaaaccaa ccatacaggt tcagcataat gtaactgaag taacagactt tcgatttgta
     2221 gaagcaaaga ttttaaaagg tcagaatggt ccacagggct taccttctcc acatagcatg
     2281 gacgatacag agactcttgt cttaatgtta gaagattcta aggctcaact tagtctgact
     2341 ttgtattata ctacttttaa taatgatgcg actattgcta gctacagtaa attagataat
     2401 aatagtaatc aggaagttgt catccataaa gatttttctt ttatggctga ttttccagct
     2461 gcagattatg aaatagcaac tctgcagggt gcttatgctc gtgaaaagac tgttagacgt
     2521 caacaggtag aacaaggaat cttttcgatt agttcaaacc gaggtgcttc tggtcatgct
     2581 caaacaccag ctcttctact atgcgaacaa ggagtcacag aggatgctgg gaatgtgttt
     2641 gctattcaac taatgtatag tggcaacttt gaagcttttg ttcaaaaaaa tcaattgaat
     2701 gaagttcggg tggctattgg cattaatcca gaaaactttt cttggaagtt agcccctgag
     2761 gaatactttg aaacaccggt agctttagtg actcattcag atcagggatt aactggtatt
     2821 agtcatgaaa gtcagaattt tgtactgaag cacattatgc taagtgaatt ttctaaaaaa
     2881 gaacgtccaa ttctaatcaa taactgggaa gctacttact ttgactttca gagagaaaaa
     2941 ctgttagagt tagcagatga agctaagaaa gttggcattg aactttttgt attagatgat
     3001 ggttggtttg gcaatcgttt tgatgataat cgtgctttag gtgattgggt tgttaatgag
     3061 gaaaaactgg gtggaagtct agaaagtctg atttcagcta tccatgaaag aggtttgcag
     3121 tttggacttt ggttagaacc cgaaatgatt tctgtagata gtgatttgta tcgtcaacat
     3181 cctgactggg ctattcaggt tcctgattat gagcatactt attctcggaa tcaattagta
     3241 cttaatcttg ccaatcctca ggtagtagaa tacttgaaaa gtgtcttaga tcaactccta
     3301 tcttatcatg agattgatta cattaaatgg gatatgaacc gcaatatcac taagctaggg
     3361 aatggattaa cttatctaga gacacagatg caatctcatc agtacatgct ggggctttac
     3421 gaactcgttt cttatctgac agagaagcac agccatattc tctttgagtc ctgctctggt
     3481 ggtggtggac gaaatgatct tggtatgata cgctatttcc cacaagtctg ggctagtgat
     3541 aatactgatg ccattgcacg tttaccaatt caatacggtt catcctatct ctatccaacc
     3601 atttctatgg gggctcatgt gtcagcagta ccgaatcatc agatgggacg aatgacacca
     3661 ttagaaacac gtggccatgt agcaatgatg ggaaatttgg gctatgagct tgatttgaca
     3721 aatttatcag atgaagagaa agctacgatt gctaatcagg tgaacttgta taaagaatta
     3781 cgaccagtag ttcagttagg acagcagtat agactaatta atcctgatac tgcatccaat
     3841 gaagcagctg tacaatttaa ttacggaaat caaacgattg taacctacgt tcgcgtttta
     3901 tctgttgtag agaccatgga aacaacttta aaattaaaag atttggatga agagggacta
     3961 tataaattac aggaaaatgg cgaagtttac tcaggtgcag aactaatgta tgcgggctta
     4021 actgttatct tatcccaagg agattttttg agtagacagt atatttttag aaaactatga
     4081 tatgtactga ccccaaaagt tagatttatt ctgtctaact tttggggtgc agttcaataa
     4141 gggtgtataa atttataaag gaggctttct aatggaatgg tataaaaaaa tcggacttct
     4201 tgcaactaca ggtttagctt tgtttgggct cggcgcttgc tccaactatg gtaaatctgc
     4261 ggatggcaca gtgaccatcg agtatttcaa ccagaaaaaa gaaatgacca aaaccttgga
     4321 agaaatcact cgtgattttg agaaggaaaa ccctaagatc aaggtcaaag tcgtcaatgt
     4381 accaaatgct ggtgaagtat tgaagacacg cgttctcgca ggagatgtgc ctgatgtggt
     4441 caatatttac ccacagtcca tcgaactgca agaatgggca aaagcaggtg tttttgaaga
     4501 tttgagcaac aaagactacc tgaaacgcgt gaaaaatgcc tacgctgaaa aatatgctgt
     4561 aaacgaaaaa gtttataacg ttccttttac agctaatgct tatggaattt actacaacaa
     4621 agataaattc gaagaactgg gcttgaaggt tcctgaaacc tgggatgaat ttgaacagtt
     4681 agtcaaagat atcgttgcta aaggacaaac accatttgga attgcaggtg cagatgcctg
     4741 gacactcaat ggttacaatc aattagcctt tgcgacagca acaggtggag gaaaagaagc
     4801 aaatcaatac cttcgttatt ctcaaccaaa tgccattaaa ttgtcggatc cgattatgaa
     4861 agatgacatc aaggtcatgg acatccttcg catcaatggc tctaagcaaa agaactggga
     4921 aggtgctggc tataccgatg ttatcggagc cttcgcacgt ggggatgtcc tcatgacacc
     4981 aaatgggtct tgggcgatca cagcgattaa tgaacaaaaa ccgaacttta agattgggac
     5041 cttcatgatt ccaggaaaag aaaaaggaca aagcttaacc gttggtgcgg gagacttggc
     5101 atggtctatc tcagccacca ccaaacatcc aaaagaagcc aatgcctttg tggaatatat
     5161 gacccgtcca gaagtcatgc aaaaatacta cgatgtggac ggatctccaa cagcgatcga
     5221 aggggtcaaa caagcaggag aagattcacc gcttgctggt atgaccgaat atgcctttac
     5281 ggatcgtcac ttggtctggt tgcaacaata ctggaccagt gaagcagact tccatacctt
     5341 gaccatgaac tatgtcttga ccggtgataa acaaggcatg gtcaatgatt tgaatgcctt
     5401 ctttaacccg atgaaagcgg atgtggatta ggagtagaga gactatgaaa aaagtattac
     5461 aaaaatattg ggcatgggct tttgtggtca tccccctctt gttacaagca attttcttct
     5521 atgtgccgat gtttcaagga gccttttaca gttttaccaa ctggacagga ttgacttaca
     5581 actacaaatt tgtcggctta aacaacttta agctcctctt catggatcca aaattcatga
     5641 atgcgattgg ctttaccgca atcattacga ttgccatggt ggttggtgag attgcactcg
     5701 ggatcttcat tgcgcgtgtc ttgaactcta aaatcaaagg ccaaaccttc ttccgtgctt
     5761 ggttcttctt cccagctgtt ttatctggtt tgacagtggc tttgatcttc aagcaagtct
     5821 tcaactacgg tcttccagcg attgggaatg cccttcatat tgaatttctc caaaccagtc
     5881 ttttagggac taagtgggga gcaatctttg cggctgtctt tgtccttctt tggcaagggg
     5941 tggctatgcc catcatcatc ttcctagctg gtttgcaatc tattccaact gagattacag
     6001 aggcagcaag gattgatggt gcgactagca agcaagtttt ctggaacatt gaattgcctt
     6061 acttgctacc aagtatctct atggtcttta tcctagccct aaaaggtggg ctgactgcct
     6121 ttgaccaagt ctttgccatg accggtggtg gtccaaacaa tgccacaacc tcacttgggc
     6181 tcttggttta taactatgcc tttaaaaaca accaattcgg ttatgccaat gccattgccg
     6241 taatcttgtt cctcttaatt gtagtgattt cgatcatcca attgagagta tctaagaaat
     6301 ttgaaattta agaggagaag catgatgaaa caagatgaaa gaaaagccct gattggcaaa
     6361 tacattctat tgattctagg atcggttctg attttagtgc cgctccttgc taccctcttt
     6421 agttccttca aacccactaa ggatattgta gataatttct ttggctttcc aaccaacttc
     6481 acatgggaca actttagccg tctcttagct gatgggattg gaggctatta ttggaactct
     6541 gtcgtcatca ctgtcttgtc tttacttgca gtaatgatct ttatccctat ggcagcctac
     6601 tccatcgctc gcaatatgag taaaagaaaa gtctttacca tcatgtatac cctcttaatc
     6661 ctcggaatct tcgtaccttt ccaagtcatc atgattccga ttacggttat gatgagtaaa
     6721 ctcggtttgg ctaatacctt tggtttgatc ttgctctact tgacctatgc gattccacag
     6781 accctctttc tctatgttgg ctatatcaaa atctcgattc cagaaagtct ggatgaagca
     6841 gcagagatcg atggggctaa tcaatttaca acctatttcc gcatcatctt cccaatgatg
     6901 aaaccgatgc atgcgacaac catgatcatc aatgcccttt ggttctggaa tgacttcatg
     6961 ttgccactcc ttgtcttgaa ccgggattcc aaaatgtgga ctctgccttt gttccaatac
     7021 aactacgcag gccaatattt caacgactac ggaccaagct ttgcctctta cgtggtcggc
     7081 attatcagta tcaccattgt ctatctcttc ttccaacgcc atatcatttc aggaatgagc
     7141 aacggggcag tgaagtaata ctcaatgaaa atcaaagagc aaactaggaa gctagccgca
     7201 ggttgctcaa aacactgttt tgaggttgtg gatgaaactg acgaagtcag taaccatacc
     7261 tacggtaagg tgaagctgac gtggtttgaa gagattttcg aagagtataa gaagagctca
     7321 tggaatctat agtctttgac acagaggaag agttcagctt gttgataatt ctatgtagaa
     7381 aacaagaaag tagtactttg tttatcacag tctatattag tacgagtttg atgataaata
     7441 gagatttaaa atgaatttga tttacgaagc cagatcatat gagacttggc ttcaattaga
     7501 aaaaggagag taatgatgcc aattcaaaat aaaaccatgt tgattaccta ttctgatagc
     7561 cttggaaata atcttaaaga cttatatgat aatttggaag agcattttgg agatgctatt
     7621 ggaggagttc accttttacc atttttccca tcaacaggtg atcgtggatt tgcgccagtt
     7681 gactacgacg aagtggactc agcttttggt gattgggagg atgttaagcg tttaggtgag
     7741 aaatattatc ttatgtttga ttttatgatt aatcatattt ctcgtcaatc caagtattat
     7801 aaggactatc aagaaaaaca tgaagccagt gaatttaaag atctcttttt aaactgggat
     7861 aagttttggc cagaaaaccg tccgacacag tctgatgtag atttaattta caagcgtaag
     7921 gatcgtgcac caaagcaaga gattgtgttt gaagatggtt cagtggaaca tttgtggaat
     7981 acctttggtg aggagcagat tgatcttgat gtgaccaaag aagtaactat ggaatttatc
     8041 cgtaagacca ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct agatgccttt
     8101 gcttatgcag tgaagaaatt ggatactaat gatttctttg tggaaccaga tatttgggat
     8161 ttattggaca aagttcgaga tatcgctgct gagtatggga cagagctttt acctgagatt
     8221 catgaacact attcgattca gtttaaaata gcagaccatg attactatgt ttatgatttt
     8281 gctcttccaa tggtgacact ttatactctt tacagttcca gaacagagcg tttggctaag
     8341 tggttaaaga tgagcccgat gaagcaattt acgacgctag atacccatga tgggattgga
     8401 gtagtagatg tcaaggatat cctgaccgat gaggagattg actatgcttc aaatgaactc
     8461 tataaggttg gagccaatgt caaacgtaag tactctagtg ccgagtataa caacttagat
     8521 atctaccaaa tcaattcaac ctactattca gcgcttggag atgatgatgt caagtatttt
     8581 ctcgctcgtc taattcaagc ttttgcccca ggtattcctc aggtttacta tgtgggtcta
     8641 ttagcaggca agaatgactt gaaattatta gaagaaacta aagaaggtcg aaatattaat
     8701 cgtcattact atagcaacga ggaaatagca aaagaagtgc aacgacctgt tgtgaaggcc
     8761 cttctcaatc tattttcttt ccgtaaccgt tcagaagcct ttgatctaga agggactact
     8821 gagatagaga caccaacagc tcacagcatt gtaatcaaac gtcaaaataa agataagtcc
     8881 gtaacagcag tagcagaaat tgacttgcaa aatcagactt atcgggtaat tgagaatgga
     8941 gttgaagtaa cattttgaag ccttgatatg gtggataaaa tagggttttt attttggaaa
     9001 acgtttcctt tgttttcaaa ttgctaaaaa agtggtacaa tataggatag cttactatta
     9061 tctgaatcag ctgatttgga gagaaaggat tcattttgaa atcaataggc tttattgaaa
     9121 agctgaaggg gttgtctagt aaagagctga ttttattggg aattatcctg agtatctttt
     9181 taccctttta tctttttgta gttgtattct gtttatatat tatcagtctg atttttacag
     9241 gagacatgaa aagtattctt cagaaaatgg gggagcattc gatgctgctt ctttttcttg
     9301 gctatagtac tgttatatcc attcttgcac aaaattggat gggtcttgtg gcttcagtag
     9361 gaatgtttct atttactatt ttctttttgc actatcagtc gattttatcc cataaattct
     9421 ttcgattgat tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct tttgccagtt
     9481 tagaacattt ccaaattgtg aagaaattta actatgcttt tctttccccc aatatgcagg
     9541 tgtggcatca gaaccgggca gaagtgacct tctttaatcc taattattat ggaattattt
     9601 gttgtttctg tattatgatt gctttctatc tgtttacaac gaccaagttg aattggttga
     9661 aagtattctg tgtgattgca ggctttgtta atctctttgg tttgaacttt actcaaaatc
     9721 gaactgcctt tcctgctatt atcgctggag caattatcta tctctttacg actattaaaa
     9781 actggaaggc cttttggctt agtattgggg tcttcgcgat tggtttgagt ttcctctttt
     9841 ctagtgattt gggagttcga atgggtactt tagactcttc tatggaagaa cgcatttcta
     9901 tctgggatgc tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa gggccattga
     9961 cctatatgca ctcttatcct cggatacatg ctccttatca tgaacatgcc cacagtcttt
    10021 atattgatac gattctgagt tacggaattg tgggtaccat tttattagtt ctgtcttctg
    10081 ttgctcctgt tcgcttgatg atggatatga gtcaggagtc ggggaaacgt ccgattatcg
    10141 gcctttatct atctttcctt acagtggttg ctgtgcacgg aatttttgac ttggctctct
    10201 tctggattca gtcaggcttt attttcttgc tagttatgtg cagtattcca ttggagcatc
    10261 gaatgttggt atcggacatg acggattaag ttttataagt ttgaaatctt ctaccttggg
    10321 taggagattt tttatacgat gggcatgtcg tgcatctgag gtgtaagtcc tctgtaggga
    10381 tccactaccg gtgaatacaa tagcgctccc caagcctgac tatcgtgagg tagcggatta
    10441 aaatggtctg gggatagatc gttttaagtc tgacgcagga aataagaatt gtcagagaaa
    10501 gcaatagtga aatcgtaggc tatgaataga aacgtgatag cgaggagcat atagtggata
    10561 aggtagcgag aaattttaaa gtataaaaga tagtcttaat ctatatatgt aaatcacaaa
    10621 agtaattgaa ttcggaataa ggttggtgtg aaaagacata gtaaactgaa tcttgaatac
    10681 tagaatgata cttctacaat attgttataa tttaatttga attccaaaat cacattgttc
    10741 cgtccgtatt tcattttact gtatgtcttc ctaaactcca aagtttctgc gtcaaatttc
    10801 ctattttcac tgtagtcttt gacacttcga acgacaattt gaatcatata ttcaaaaaaa
    10861 aatgaaaaat ttgtgccaac aagcctaaat att
//
LOCUS       DAVLFB010000069        10834 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLFB010000069 DAVLFB010000000
VERSION     DAVLFB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.069"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            560..1552
                     /locus_tag="VXD58_001538"
     CDS             560..1552
                     /locus_tag="VXD58_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676210.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1554..2372
                     /locus_tag="VXD58_001539"
     CDS             1554..2372
                     /locus_tag="VXD58_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV0676211.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2374..3159
                     /locus_tag="VXD58_001540"
     CDS             2374..3159
                     /locus_tag="VXD58_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676212.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3198..3476
                     /locus_tag="VXD58_001541"
                     /pseudo
     CDS             3198..3476
                     /locus_tag="VXD58_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
     gene            3461..3658
                     /locus_tag="VXD58_001542"
     CDS             3461..3658
                     /locus_tag="VXD58_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676213.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
     gene            complement(4039..4137)
                     /locus_tag="VXD58_001543"
                     /pseudo
     CDS             complement(4039..4137)
                     /locus_tag="VXD58_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4344..10760
                     /locus_tag="VXD58_001544"
     CDS             4344..10760
                     /locus_tag="VXD58_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV0676214.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHSAMETSQDFKEKKIAVIKEKEVVSKTPVIDTKTSNEENSNKS
                     QGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETIPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNFWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGQSFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLEKPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQDPDLFAFNNQGVHAESTSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHFI
                     RGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEYGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISYY
                     LVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKSLFN
                     IHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDDGTD
                     LEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKATKY
                     DFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSEYP
                     YVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVT
                     TYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVLMS
                     ALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAI
                     KEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNE
                     SVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQ
                     TGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDG
                     EIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSEL
                     KPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVI
                     NLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
BASE COUNT         3785 a         1655 c         2187 g         3207 t
ORIGIN      
        1 tccaaggtaa caagtctaga tcagattgaa gctgataaaa cgatacaaag aaaatattca
       61 agtgagctaa aaaaatttat tggattttat aatgagatta tttgtgagga aaatagtttc
      121 ctacatgtac gaaagaggtg gtcgagttgg tttaggtagt cgatgcgtga gttgataatt
      181 ctcagggtat ggacttcttt ttcatgaatg aggtaaaaga gcaggtattg tttagagaca
      241 atcattctga gcatattttc tggatagagg gagtatccga ttttatgatc aaagttaata
      301 ccgccctctg gtgagaagat aagtaggttg gtaatttaaa ctattaaaca gaatttttga
      361 ttaaaagtat tattttatga gagaaatcct aatttcacaa tccataggca aacgcttgca
      421 tttcgttttt tattggacta taataggttg gtataaagcc ttttgtagta ataaaatgta
      481 gaaggtgtag aaagcaagga tttagaatat ttgtagttaa aaacacaatg ttgctattcc
      541 ttacgatagg gagatagata tggcaatgat agaagtggaa catcttcaga aaaattttgt
      601 gaagactgtt aaggaaccgg gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa
      661 gcagaccttt gaagcggtca aggatttgac ctttgaggtt ccaaaagggc agattttagg
      721 atttatcggg gcaaatggtg ctgggaagtc gacaaccatt aaaatgctga caggaatttt
      781 gaaaccaaca tctggttttt gtcggattaa cggcaagatt ccccaggaca atcggcaaga
      841 ttatgtcaaa gatattggcg tagtctttgg acaacgcacc cagctatggt gggatttggc
      901 tctgcaagag acctacactg tcttaaaaga gatttatgat gtgccaaact cgctctttca
      961 taagcgcatg gactttttga atgaagtctt ggatttgaag gactttatca aggatcccgt
     1021 gcggactctt tcactgggac aacggatgcg ggcggatatt gcggcctcct tgctccacaa
     1081 tcccaaggtt ctttttttag atgagccgac cattggtttg gacgtttcgg ttaaggataa
     1141 tattcgtcgg gcaattactc agatcaatca agaggaagaa actaccattc ttttgaccac
     1201 tcacgatttg agtgatattg agcaactttg tgatcggatt ttcatgattg acaaggggca
     1261 agagattttt gatggaacgg tgagccaact caaggagacc tttggtaaga tgaagactct
     1321 ctcttttgaa ctgctaccag gtcaaagtca tctcgtctct cactatgaag gtctgtctga
     1381 tatgaccatt gatagacaag gaaacagcct caacattgaa tttgatagtt ctcgctacca
     1441 gtcagctgac attatcaagc aaaccctgtc tgattttgaa atccgcgatt tgaagatggt
     1501 ggatacggat attgaggata ttatccgtcg cttctaccga aaggagctct aggatgatca
     1561 aattgtggag acgttataaa ccctttatca atgcaggggt tcaggagttg attacttacc
     1621 gagtcaactt tattctctat cggattggcg atgtcatggg ggcttttgtg gccttttatc
     1681 tctggaaggc tgtctttgat tcttcgcaag agtctttgat tcagggcttc agtatggcgg
     1741 atatcaccct ctacatcatc atgagttttg tgaccaatct tctgactaga tccgattcgt
     1801 cctttatgat tggggaggag gtcaaggatg gctccattat catgcgtttg ttgcgaccag
     1861 tgcattttgc ggcctcctat cttttcaccg agcttggttc caagtggttg atttttatca
     1921 gcgttggcct tccattttta agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta
     1981 ttgtagaggt gctaggatta actgtccttt atctttttag cttaacgctc gcctatctga
     2041 ttaacttttt ctttaatatt tgctttggat tttcagcctt tgtgtttaaa aatctttggg
     2101 gttccaacct acttaagact tccatagtgg cttttatgtc ggggagtttg attcccttgg
     2161 cattctttcc aaaggttgtt tcagatattc tttccttttt gcctttttca tccttgattt
     2221 atactccagt tatgatcatt gttggaaaat acgatgccag tcagattctt caggcactcc
     2281 ttttgcagtt cttctggctc ttagtgatgg tgggattgtc tcagttgatt tggaaacggg
     2341 tccagtcctt tatcaccatt caaggaggtt agtatgaaaa aatatcaacg aatgcatctg
     2401 atttttatca gacaatacat caaacaaatc atggaatata aggtggattt tgtggttggt
     2461 gtcttgggag tctttctggc tcaaggcttg aatctcttgt ttctcaatgt catctttcaa
     2521 catattccat tcctagaagg ctggaccttt caagagatag ctttcattta tggattttcc
     2581 ttgattccca agggaatgga ccatctcttt tttgacaatc tctgggcact agggcaacgc
     2641 ctagtccgaa aaggagagtt tgacaagtat ctgactcgtc ccatcaatcc tctctttcac
     2701 atcctagttg aaacctttca gattgatgcc ttgggtgaac tcttagtcgg tggtatttta
     2761 ttgggaacaa cagtgaccag cattgtttgg actcttccaa aattcctgct tttcctagtt
     2821 tgtattcctt ttgcgacctt gatttatact tctcttaaaa tcgcaacagc cagtatcgcc
     2881 ttttggacta agcagtcagg cgccatgatt tacatcttct atatgttcaa tgactttgct
     2941 aagtatccga tttctattta caattctctt cttcgttggt tgattagctt tatcgtgcct
     3001 ttcgccttta cagcctatta tccagctagc tatttcttac aggaaaagga tgtgttcttt
     3061 aacgtaggag gtttgatgtt gatttctctg gttttctttg ttatttccct taaactttgg
     3121 gataagggct tagattccta cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat
     3181 caagaaagga acttatgatg tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg
     3241 cagttgtcgc tgaggttttg aaggatttgc cagaatggtt tggaatccca gaaagcacat
     3301 aagcctatat aaaaggaacc acgacactgc aagtttggac cgcctatcag gagagtgatt
     3361 tgactggatt tgtaagctta tcctattcga gtgaagattg tgcagagatt gattgtctcg
     3421 gcgtaaaaaa gcttatcaag gtagaaaaat tgggagccaa ttgcttgcta ctttagagag
     3481 tgaagctcgt aaaaaagttg gttatctgca ggtcaaaaca gtggcagaag gttctaataa
     3541 agattatgat cgaacaaatg acttttatcg aggtcttggc tttaaaaagt tagagatttt
     3601 tcttcaacta tggaatccgc aaaatccttg tcagattttg attaaaaagc ttgaataata
     3661 ttacttgaca tctattctca gagtgctata ctgtaagtgt aatcgccgat ttagcttagt
     3721 tggtagagca aggcactcgt aaagcctagg ttataggtag ataaacgact gaggatttga
     3781 aaaaatagat aggtagaaga taaccgttaa gccttattct tagcggttat ttatattgtt
     3841 taatagcgct gatatttcat caattatgcc tgttttcgtg tttctggtag ttgttcaagt
     3901 ttattgctac tatttttgat ggtatgaatg tgcttataat gtatcccggt taacgaaagt
     3961 tttggactta tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtgcgt
     4021 atggttactg actacgtcag ttctatccac aacctcaaaa cagtgttttg agcaatctgc
     4081 ggctagtttc ctagtttgct ctttgatttt cattgagtac taatcttatt tttaacgtgt
     4141 gctaaaaaat aaattaatgc atgttaattt ttttgactta aattttttta aatgatatac
     4201 tgtttttata gagagataac aattatatat aaatgtacta ctctattttc tagataatac
     4261 ttagtaaaac tttttataac aaaggctagc aaagttaaag tttattatct attggaagtt
     4321 atataaatat gtaaggaatt aaaatgaaaa aaagtacagt attgtcatta accacagctg
     4381 cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca tctagctctg
     4441 aagatgcttt aagcatctct gataaagaaa aagtagtagt agataaggaa acagaaaata
     4501 aagagaaaca taaagatatc catagtgcta tggaaacttc acaggatttt aaagagaaga
     4561 aaatagcagt cattaaggaa aaagaagttg ttagtaaaac tcctgtgata gacactaaaa
     4621 ctagcaatga agaaaattcc aataaatccc aaggagatca tacggactca tttgtgaata
     4681 aaaacacaga aaatcccaaa aaagaagata aagttgtcta tattgctgaa tttaaagata
     4741 aagaatctgg agaaaaagca atcaaggaac tatccagtct taagaataca aaagttttat
     4801 atacttatga tagaattttt aacggtagtg ccatagaaac aattccagat aacttggaca
     4861 aaattaaaca aatagaaggt atttcatcgg ttgaaagggc acaaaaagtc caacccatga
     4921 tgaatcatgc cagaaaggaa attggagttg aggaagctat tgattaccta aagtctatca
     4981 atgctccatt tgggaaaaat tttgatggta gaggtatggt catttcaaat atcgatactg
     5041 gaacagatta taggcataag gctatgagaa tcgatgatga tgctaaagcc tcaatgagat
     5101 ttaaaaaaga agacttaaaa ggaactgata aaaatttctg gttgagtgat aaaatccctc
     5161 atgcgttcaa ttattataat ggtggcaaaa tcactgtaga aaaatatgat gatggaaggg
     5221 attattttga cccacatggg atgcatattg cagggattct tgctggaaat gatactgaac
     5281 aagatatcaa aaactttaac ggcatagatg gaattgcgcc taatgcacaa attttctctt
     5341 ataaaatgta ttctgacgca ggatctgggt ttgcgggtga tgaaacaatg tttcatgcta
     5401 ttgaagattc tatcaaacac aacgttgatg ttgtttcggt atcatctggt tttacaggaa
     5461 caggtcttgt aggtgagaaa tattggcaag ctattagggc attaagaaaa gcaggcattc
     5521 caatggttgt cgctacgggt aactatgcga cttctgcttc aagttcttca tgggatttag
     5581 tagcaaataa tcatctgaaa atgaccgaca ctggaaatgt aacacgaact gcagcacatg
     5641 aagatgcgat agcggtcgct tctgctaaaa atcaaacagt tgagtttgat aaggttaaca
     5701 ttggaggaca aagttttaaa tacagaaata taggggcctt tttcgataag aataaaatca
     5761 caacaaatga agatggaaca aaagctccta gtaaattaaa atttgtatat ataggcaagg
     5821 ggcaagacca agatttgata ggtttggatc ttaggggcaa aattgcagta atggatagaa
     5881 tttatacaaa ggatttaaaa aatgctttta aaaaagctat ggataagggt gcacgcgcca
     5941 ttatggttgt aaatactgta aattactaca atagagataa ttggacagag cttccagcta
     6001 tgggatatga agcggatgaa ggtactaaaa gtcaagtgtt ttcaatttca ggagatgatg
     6061 gtgtaaagct atggaacatg attaatcctg ataaaaaaac tgaagtcaaa agaaataata
     6121 aagaagattt taaagataaa ttggagcaat actatccaat tgatatggaa agttttaatt
     6181 ccaacaaacc gaatgtaggt gacgaaaaag agattgactt taagtttgca cctgacacag
     6241 acaaagaact ctataaagaa gatatcatcg ttccagcagg atccacatct tgggggccaa
     6301 gaatagattt acttttaaaa cccgatgttt cagcacctgg taaaaatatt aaatccacgc
     6361 ttaatgttat taatggcaaa tcaacttatg gctatatgtc cggaactagt atggcgactc
     6421 caatcgtagc agcttctact gttttgattc gaccaaaatt gaaggaaatg cttgaaaaac
     6481 ccgtattaaa aaatcttaaa ggtgatgata aaatagacct tacaagtctt acaaaaatag
     6541 ccctacaaaa tactgcaaga cctatgatgg atgcaacttc ttggaaagaa aaaagtcaat
     6601 actttgcatc acctagacag cagggggcgg ggctaattaa tgtggccaac gctttgagaa
     6661 atgaagttgt agcgactttc aaaaacacgg attctaaagg tttggtaaat tcatatggtt
     6721 ccatttctct taaagaaata aaaggtgata aaaaatactt tacaattaag cttcacaata
     6781 catccaacag gcctttaacc tttaaagttt cagcatcagc ggtaactaca gattctctaa
     6841 ctgacagact aaaactggat gaaacatata aagatgaaaa atctccagat ggtaagcaaa
     6901 ttgttccaga aattcatcca gaaaaagtca aaggggcaaa tattacattt gagcatgaca
     6961 ctttcactat aggggcaaat tctagctttg atttaaatgc ggttataaat gttggggagg
     7021 ctaaaaataa aaataaattt gtagaatcat ttattcattt tgagtcagta gaagaaatgg
     7081 aagcattaag ctccaatggc aagaaaaccg acttccaacc atctttatca atgcccctaa
     7141 tgggatttgc tgggaattgg aaccacgaac caatccttga taaatgggct tgggaagaag
     7201 gatcaaaatc aaaaacaatg gaaggttatg atgatgacgg taaaccaaaa attccaggaa
     7261 ccttaaataa ggggattggt ggagaacatg gtatagataa atttaatcca gcaggagtta
     7321 tccaaaatag aaaagataaa aatacaacat ctctagatca agatccagac ttatttgcct
     7381 ttaacaacca aggtgttcat gcagaatcca ctagtggttc taagattgct aacatttatc
     7441 ctttagattc aaatggaaat cctcaagatg ctcaactaga aagaggacta actccatctc
     7501 cacttgttct aagaagtgca gaagaaggac taatttcaat tgtaaacacc aacaaagaag
     7561 gagaaaacca aaaagacctt aaagttgtat caagagagca cttcataaga ggaattctaa
     7621 attctaaggg aaatgacgct aagggtatca aatcatccaa actaaaagtt tggggtgact
     7681 tgaaatggga tggactcatc tataacccaa gaggtagaga agaaaatgcc ccagaaagta
     7741 aggataacca agatccagct actaagatac gaggacaatt tgaaccgatt gcggaaggtc
     7801 aatatttcta taaatttaaa tatagattaa ctaaagatta cccatggcag gtttcctata
     7861 ttcctgtaaa aattgataac acagccccta agattgtttc ggttgatttt tcaaatcctg
     7921 aaaaaattaa gctaatcaca aaggatactt atcacaaggt aaaagatcaa tacaagaatg
     7981 aaactttatt tgctagagat caaaaagaac atcctgaaaa atttgatgag attgcaaatg
     8041 aggtgtggta tgcaggagct gctcttgtta atgaatatgg agaggttgag aaaaatcttg
     8101 aagtaactta tgcaggtgag ggtcaaggaa gaaataggaa gcttgataaa gatgggaata
     8161 ctatctatga aattagcgga gcaggagatc taagaggaaa aatcatcgaa gtaatcgccc
     8221 tagatggagc aagcaacttt accaaaatcc ataggattaa atttgctaat caagctgatg
     8281 aaaaagggat gatttcctat tatctagtag atcctgatca ggattcatct aaataccaaa
     8341 agcttggcga aattcctgaa tctaaattta agaatttaaa aaatgttaaa gatgatagcc
     8401 taaacaaaga gacagctgag gtggaaaata accttttagt agataatcag tctatcgaag
     8461 gaaaatctct ctttaatatt cataaaacta tttcaacaat tagagacttt gaaaataaag
     8521 acctaaagaa actcattaaa aagaaatata agcaagaaga cgactttgta aatggtggaa
     8581 caagaacagt agagcgtgat tataaatacg atgataaagg aaatataata gcctacgatg
     8641 atgggactga tctagaatat gaaactgaga aacttgacga aatcaaatca aaaatttatg
     8701 gtgttctaag tccgtctaaa gatggacact ttgaaattct tggaaagata agcaatgttt
     8761 ctaaaaatgc caaggtatat tatgggaata actataaatc tatagaaatc aaagcgacca
     8821 agtatgattt ccactcaaaa acgatgacat ttgatctata cgctaatatt aatgatattg
     8881 tggatggatt agcttttgca ggagatatga gattatttgt taaagataat gatcagaaaa
     8941 aagctgaaat taaaattaga atgcctgaaa aaattaagga aactaaatca gaatatccct
     9001 atgtatcaag ttatgggaat gtcatagaat taggggaagg agatctttca aaaaacaaac
     9061 cagacaattt aactaaaatg gaatctggta aaatctattc tgattcagaa aaacaacaat
     9121 atctgttaaa ggataatatc attctaagaa aaggctatgc actaaaagtg actacctata
     9181 atcctggaaa aacggatatg ttagaaggaa atggagtcta tagcaaggaa gatatagcaa
     9241 aaatacaaaa ggccaatcct aatctaagag ccctttcaga aacaacaatt tatgctgata
     9301 gtagaaatgt tgaagatgga agaagtaccc aatctgtatt aatgtcggct ttggacggct
     9361 ttaacattat aaggtatcaa gtgtttacat ttaaaatgaa cgataaaggg gaagctatcg
     9421 ataaagacgg aaatcttgtg acagattctt ctaaacttgt attatttggt aaggatgata
     9481 aagaatacac tggagaggat aagtccaatg tagaagctat aaaagaagat ggctccatgt
     9541 tatttattga taccaaacca gtaaaccttt caatggataa gaactacttt aatccatcta
     9601 aatctaataa aatttatgta cgaaatccag aattttattt aagaggtaag atttctgata
     9661 agggtggttt taactgggaa ttgagagtta atgaatcggt tgtagataat tatttaatct
     9721 acggagattt acacattgat aacactagag attttaatat taagctgaat gttaaagacg
     9781 gtgacatcat ggactgggga atgaaagact ataaagcaaa cggatttcca gataaggtaa
     9841 cagatatgga tggaaatgtt tatcttcaaa ctggctatag cgatttgaat gctaaagcag
     9901 ttggagtcca ctatcagttt ttatatgata atgttaaacc cgaagtaaac attgatccta
     9961 agggaaatac tagtatcgaa tatgctgatg gaaaatctgt agtctttaac atcaatgata
    10021 aaagaaataa tggattcgat ggtgagattc aagaacaaca tatttatgta aatggaaaag
    10081 aatatacatc atttgatgat attaaacaaa taacagacaa gacactaaac attaagattg
    10141 ttgtaaaaga ttttgcaaga aatacaaccg taaaagaatt cattttaaat aaagatacgg
    10201 gagaggtaag tgaattaaaa cctcataggg taactgtgac cattcaaaat ggaaaagaaa
    10261 tgagttcaac gatagtgtcg gaagaagatt ttattttacc tgtttataag ggtgaattag
    10321 aaaaaggata ccaatttgat ggttgggaaa tttctggttt cgaaggtaaa aaagacgctg
    10381 gctatgttat taatctatca aaagatacct ttataaaacc tgtattcaag aaaatagagg
    10441 agaaaaagga ggaagaaaat aaacctactt ttgatgtatc gaaaaagaaa gataacccac
    10501 aagtaaacca tagtcaatta aatgaaagtc acagaaaaga ggatttacaa agagaagatc
    10561 attcacaaaa atctgattca actaaggatg ttacagctac agttcttgat aaaaacaata
    10621 tcagtagtaa atcaactact aacaatccta ataagttgcc aaaaactgga acagcaagcg
    10681 gagcccagac actattagct gccggaataa tgtttatagt aggaattttt cttggattga
    10741 agaaaaaaaa tcaagattaa gacaaaagct atagaaaaaa atggtttatg tactgagatt
    10801 agatagtgag gtgatgacat agttttgtga aaac
//
LOCUS       DAVLFB010000070        10766 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLFB010000070 DAVLFB010000000
VERSION     DAVLFB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.070"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(44..1366)
                     /locus_tag="VXD58_001545"
     CDS             complement(44..1366)
                     /locus_tag="VXD58_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV0676215.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFTFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1457..1545)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1709..4045)
                     /locus_tag="VXD58_001546"
     CDS             complement(1709..4045)
                     /locus_tag="VXD58_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV0676216.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKGQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQIGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4149..4697)
                     /locus_tag="VXD58_001547"
     CDS             complement(4149..4697)
                     /locus_tag="VXD58_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV0676217.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4694..4996)
                     /gene="zapA"
                     /locus_tag="VXD58_001548"
     CDS             complement(4694..4996)
                     /gene="zapA"
                     /locus_tag="VXD58_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV0676218.1"
                     /translation="MANLNRFKFTFGKKTLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5083..5964
                     /locus_tag="VXD58_001549"
     CDS             5083..5964
                     /locus_tag="VXD58_001549"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV0676219.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5968..6582
                     /gene="lepB"
                     /locus_tag="VXD58_001550"
     CDS             5968..6582
                     /gene="lepB"
                     /locus_tag="VXD58_001550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV0676220.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6662..6784
                     /locus_tag="VXD58_001551"
     CDS             6662..6784
                     /locus_tag="VXD58_001551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HEV0676221.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            6861..9227
                     /locus_tag="VXD58_001552"
     CDS             6861..9227
                     /locus_tag="VXD58_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV0676222.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYIFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILDAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(9275..10558)
                     /locus_tag="VXD58_001553"
     CDS             complement(9275..10558)
                     /locus_tag="VXD58_001553"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV0676223.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3072 a         2438 c         2196 g         3060 t
ORIGIN      
        1 aattatttat aggcaaaaaa atcacacgag ctgtgtgatt ccattatttg tcaaaatact
       61 ttttagtttc agcaataacg actggcgaca agaccaagag ggcaatcaag tttggcagag
      121 ccatcaaggc gttaacgata tctgcgataa tccagaccat atccaactcg ataaatcctc
      181 ctaacaagac catgagcaca aaaaacacac ggtagagcca gataaagcga gccccaaaga
      241 ggaactcaaa acagcgttct ccgtaatagt tccaacctag aatcgttgta aaggtaaaaa
      301 gtacaaggaa gatggtcaag agggcaggcc caaagtgtga aaagactgtt gagaaagctg
      361 actgagtcaa ggcaacccca ttcaagtcac cactccaaac tccagttacc aagatggtca
      421 aaccagttag agtacaaatg atgagggtat caataaaggt tcctgtcatg gaaatcaaac
      481 cttgctctac tggttcattt gtcttggcag ctgcagctgc aataggagca gaacccagac
      541 cagattcgtt tgagaacaca ccacgcgcca caccattttg aatagccatc cgaacgctag
      601 caccagcaaa tccacctacc gcagcaaggg gactaaaagc tgaggtaaag actaaagcga
      661 ttgtgccagg gatttttccg atattaaaga aaataactgt aagagttcct aagatataaa
      721 tgagggccat aaaaggaaca acagtagttg aaaccttaga aatagacttg agtccaccaa
      781 agactgcaat cgctacaaag acagacaaga cgagagctgt gatggctggc gaaatcgtcg
      841 ttgtattttg gatagattct gtaatcgagt tgacttgggt gaaggttccg attcccaaga
      901 gagcaaccaa tactcctgct actgcaaaca aaacagcaag tggtcgccac ttttctccca
      961 tccctagaag gatatagtgc atgggacctc ccgctactgc accatggtcg tccttggtgc
     1021 ggtatttgat ggccaagagt ccttccgcat acttggtagc cattccaaag aaagccgcca
     1081 tccacatcca aaatagagct cctggtccac caaccttgat agccgtcgca actcctatga
     1141 tatttcctgt tccaacagtt gatgccaagg ctgtacacag agctgcaaaa ctggatacat
     1201 caccatgtcc cttatcctgg ataaaaataa gctgaaaggc cttgggcaga cgcaaaacct
     1261 gcaagagtcc tagccgcatg gttaggtaaa tccctgttcc gaccaataaa atcaagaggg
     1321 gcggtcccca agcaaaagca tcgattgatt taagcaattc taacattttc ttctcctatc
     1381 gtttcaaccc caaaagaaag agcacatgca agatacatgt actctggaat gcttagataa
     1441 atgctaaaaa gcggtctatc ctagctctgt ccttttacct gagagtttga gcagttgcct
     1501 gccttgcccc ttcggtgcct ttacggtctc tccagagttc cgtccattta cagtcatgga
     1561 aaatcaaacg attccccact tctattaaac ttcattcggt gttggtattt aattgattct
     1621 aattttacaa aaaatgttgg cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag
     1681 tttttacttt ataaagtcca gaatactgct atcctttaaa agtgacaata gtcgcaccac
     1741 tgcctccagc attttgtggg gcatagccga aactcttgac atgtttgttt ctttgcaagt
     1801 atttggtaac tccttcacgg atgactcctg ttccgatacc atggatgata tcaacttgag
     1861 ccatattgtt aagcaaggct tggtcgatga aggtatctag ctcattcatg gcttcttcat
     1921 agcgcttgcc tcgaagatcc agtctagctt gaggtcctcg cccagaagtt cgtttcacaa
     1981 cattgacctg tttcttcttg actggttttt cttgctgggc ttgaacaaga tcaaactctt
     2041 tctcttccaa ggtcatctta atcaagccaa tttgggcttc ccagcggccg tccttgagtt
     2101 gactggtcaa ggtaccacgc tgaccataac tgagaaccac gatatcatct cccacctttg
     2161 gagctcgttt tttcttggcc ttttgaagga ccttgttttt agacaagtcc actttttcag
     2221 gagccaattt tttcaacttg gccttggctt caatgatttc gtggggcttg agttgggatt
     2281 tactgtggag atttttgaga atctggtcac tttcacttag ggccatatcc acaatctcag
     2341 cagcctgttc acgcgccttg ttaagctcgg tttccttttc acgattaagc tcgttgtaga
     2401 gtttttttag cgcacggttc atcttgagat tttcttgctc cacctcacgg atattgtcca
     2461 agcgtttgcg gctttccagc gtctgctctt ctaattgctc aatgatacga ttgacgtcat
     2521 tgtcctgatc gatctgctga ctggcatctc ctacgataac ttcagatagg cctagacgtt
     2581 tggcaatttc aaaggcatta cttcggccag gaacaccctg cataaagcga taggtcgggc
     2641 gaagagttgc agtatcaaac tccatactgg cattttgcac aaaggctgtc tcaataccgt
     2701 aggccttgag ttctggatag tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt
     2761 cctccagaat agccatggca agggctgctc cctcttgggg atcagtacca gcccccaact
     2821 catccaaaag taagagtgaa tgttggttga ccttgccaag aatatccacg atattggtca
     2881 tatggctaga gaaggtagac aagctctgct caatagactg ctcatctcca atatcagcaa
     2941 agatttcttc aaaaatacca acacgacttc ccttgtctgc taaaatcggc aatcctgact
     3001 gggccatgac ctgtgtcaag cccagagttt taagcatgat ggtcttccca cctgtattgg
     3061 gacctgtaat gacaatagct gttaaatctt gaccaaaata gacatcattt gcgacggcat
     3121 ttttgaccaa aggatggcag acatggagca gttgaatctc ttgattttct gacagctgag
     3181 gcacgactgc ttgtctttct tgaataaatc gaaccttggc acgaatcaag tccagatgac
     3241 cgataatcca agcgtcatta gcaatctcag ccgcatgagg gcggacacgc tcagaaattt
     3301 cttggagaat gcgaagcatt tcatagcgct catctgctcg cagactagca atttcttcgc
     3361 tcagtttgac tacctcacgt ggttcgatat agacggtgtt tccactagca gaaatatcat
     3421 gaacgacacc tgcaatctta ttgcggtagg tgtttttgac tggtaaaacc tgacggccat
     3481 ttctgctagc aacaattcct tccgtcaaca tctgcccttt ttgcttgagc aagtcttgta
     3541 aaacatcgcg tacctgactc tcgctatcat gtatttttcg acggattcgc gccaattctt
     3601 cactggcaaa attttcaatg aaacccgcat cattaaaggc ctgaagattt ccttgtaatt
     3661 gcggaaaatc atgtaatttc tcaaaccaaa gggctaattc ttccaagctg acattttcca
     3721 gattggcgta aaaactttga agttctcggc tggcaagaag cacgcgtttc aagagtagga
     3781 actcctcgat attgagatcc gctcccatct ccaacctctt gcagactcct gcaatttcct
     3841 tagttgagag aatagtaaaa tgcggttgct cgacgaaaag agcctgcatt tctttcatct
     3901 cagcaaaagc ctgtttgatt ttatctgctt tggcagtcgg agccagctgt ctcaattgct
     3961 ccaagccctg ctcggtcaac aaatgaggct caaacaaggc cttgacctta tcgaactcta
     4021 atgtttctaa tattttctta ttcatcttta tttccttgtc tttgaatgtc taggtgtggt
     4081 agctttttac attctgatag attcgagtca aatctgtaaa caaagggcta ggaaaactcc
     4141 tgcccttttt atccgattaa atttgtcacc cagatttgtt tgagccaact ggttgttatc
     4201 ggtatgctct ggatgatgtg ttttgcgacg atactctttt caagaggatt ttgtataact
     4261 gccataggga tggtcgccaa gattgtcaag gccatttgca agacaaataa ggtcaccaac
     4321 atggacaaga tacctgctga aacttggaac aacttaccac ccagtttttt gctaggaatc
     4381 aagtgtaaga gaagaccaag caaacgacca atgctataga caatcccaaa tacaagcaag
     4441 tagccgatac ctgcataaaa gaccttatcc agctgaaaga gttgatccga tgggaaaaag
     4501 aaagtcccct gaccttcctg cgaatttgca taagggagca ataaatggaa ttgctctcca
     4561 agccccttat aaaactggcc agccataaaa gccgatgcca tggctgaaat caggtaataa
     4621 acctgtaaga gcaggcctct ccgatagccg atataaaatc cccaaaccaa gaccaataga
     4681 agaaggaatg aaatcataag gaatcctcaa tcttgctctg ttcttgctta caagtcacaa
     4741 gcttgtgacg gagttcttct agctcttgct ccttatcgtc aaattcaatc tcacggctga
     4801 gctgagttga taaacagttg actgccaaca aaagagcgat tgtttcatca tctgcgctag
     4861 gcatttgttc tttaattgct tggtattttt ctgtcgcaac cttagcgatt tcctccataa
     4921 aaaggttatc atgttcgctt gtcaaggtta acgttttttt cccgaatgta aatttgaatc
     4981 gatttagatt tgccataaaa ttcacctcac gatattatac caaaattcgc taattttgtc
     5041 agtttttaca aattttactg cttttgtggt acaatagaaa ctatggcaag tatcacactc
     5101 acaccaagcg aaaaggatat tcaggctttt cttgaacact atcaaaccag tctggctcct
     5161 agcaagaatc cctatatccg ctactttttg aaactacctc aagcaacggt ttctatctat
     5221 acttctggaa aaatcttgct tcagggtgaa ggggctgaaa aatacgccag tttctttggc
     5281 tatcaagctg tagagcaaac cagcggacaa aatcttcctt taattgggac agatgaggtg
     5341 ggaaatggtt cctactttgg tgggcttgca gttgtggctg cctttgtcac acctgaccag
     5401 cacgactttt tacgaaaact cggtgtgggg gattctaaga ctctgaccga ccaaaagatc
     5461 cgtcagattg ctcctattct caaggaaaaa attcagcacc aggcactcct tctctcaccc
     5521 agcaagtaca acgaggtcat cggagaccgc tacaatgctg tttcggttaa ggttgccctc
     5581 cataatcagg ctatctatct cctccttcaa aaaggtgttc agcctgagaa aattgtgatt
     5641 gatgccttta ccagtgctaa aaattatgac aagtacttgg cacaagaggc caatcgtttc
     5701 agcaatccta tcagcttaga agaaaaggct gagggcaaat acttggctgt cgcagtttct
     5761 tctgtcattg cgcgtgatct ctttctggaa aatcttgaaa atctgggacg agaactgggt
     5821 tatcagctcc caagtggagc tggaacagct tctgacaagg tggctagcca gattttgcaa
     5881 gcctatggta tgcagggact cagcttctgc gccaaattgc actttaaaaa cactgaaaaa
     5941 gcgaaaaaac gcttagaaag gtaagttatg aattcattta aaaatttctt aaaagagtgg
     6001 ggattgttcc tcctaattct gtcattacta gctttaagtc gtatcttttt ttggagcaat
     6061 gttcgcgtag aaggacattc catggatccg accctagcgg atggcgaaat tctcttcgtt
     6121 gtaaaacacc ttcctattga ccgttttgat atcgtggtgg cccatgagga agatggcaat
     6181 aaggacatcg tcaagcgcgt gattggaatg cctggcgaca ccattcgtta cgaaaatgat
     6241 aaactctaca tcaatgacaa agaaacggac gagccttatc tagcagacta tatcaaacgc
     6301 ttcaaggatg acaaactcca aagcacttac tcaggcaagg gctttgaagg aaataaagga
     6361 actttcttta gaagtatcgc tcaaaaagcc caagccttca cagttgatgt caactacaac
     6421 accaacttta gctttactgt tccagaagga gaataccttc tcctcggaga tgaccgcttg
     6481 gtttcgagcg acagccgcca cgtaggtacc ttcaaagcaa aagatatcac aggggaagct
     6541 aaattccgct tctggccaat cacccgtatc ggaacatttt aagagaccta agaggccgag
     6601 aatcacaaat ctcagcctct tcttctatcg tgagaagtga ttgtttattg acaaatactc
     6661 aatgaaaatc aaagaacaaa ctaggaagct agccgtaggc tgcttaaaac agtgttttga
     6721 ggttgtggat agaactgacg aagtcagctc aaaatactgt tttgaggttg cagatggaag
     6781 ctgacgtggt ttgaagatat tttcgaagag tataaaataa aaacaatccc aactctcacc
     6841 tattcatgca aaggaatttt atggaagttt atttttcagg aaccattgaa cggattattt
     6901 ttgaaaatcc cagcaatttt tatcgcatcc tcctcctaga aatcgacgat acggacgcag
     6961 aggattttga tgattttgaa atcattgtca caggaaccat ggctgatgta attgagggcg
     7021 aagactatat tttttggggg caaattgtcc agcactccaa gtatggagaa caactgcaaa
     7081 tcagtcgtta tgatcgcgca aaaccaacta gtaagggatt ggtcaagtac ttttcaagta
     7141 gccatttcaa ggggattggt ctcaagacag ctcagaaaat cgtagatacc tatggcgaaa
     7201 ataccattga tgaaattttg caacacccag aaaagttaga aggcatcgca ggactctctg
     7261 ccaaaaatcg cgaggctttc gtctccactc tccgtctcaa ctacggaacg gagatggttt
     7321 tggccaaact agccaactac ggcattccca acaaactagc ctttcagatt caagactttt
     7381 acaaggaaga aacccttgat gtggttgaaa attatcccta ccagctggtt gaggatatca
     7441 agggtttggg ctttacaatt gctgaccaac tagctgagga actaggcatc gaaagtcagg
     7501 ctcctgaacg cttccgcgcc ggtctagttc acagtctttt tcaggcctgt atggaaacag
     7561 gggacaccta tgttgaagca cgggatttgc tggaacaaac ccttactctc cttgagtctt
     7621 cccgtcccgt ggaactagac cccagccaag ttgcccaaga gctctcctac ctgatcgaag
     7681 aagacaaggt tcagcagatt gataccaaga tctttgacaa cagcctcttt ttcgctgagg
     7741 aaggcatccg cagtcacttg gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt
     7801 tagaaaccat tcaaaaacat atcactacta tcgaggaaga gctgggaatt gagtatgata
     7861 acattcaaaa acaggctatc tgtgacgcta tccagaacaa ggtctttatc ctgacaggtg
     7921 ggcctggtac tggtaagaca actgttatca atggaatcat cgctgtttat gcccttttag
     7981 aaggacttga cttcaggaag aaaaacaatc tgcccattct tcttgctgct ccaactggac
     8041 gagccgctcg ccgcatgaat gaattgacag gtttgcctag cgcgaccata caccgccact
     8101 tgggaatgac aggtgacgat gataccagtc atctggaaga ttacctagat gctgacttta
     8161 tcatcgtgga tgaattctct atggtggata cttggctggc caatcaactc ttctccaata
     8221 tctcttctaa cagtaagatc ctcatcgtgg gtgacagtga tcagctacct tctgtcagtc
     8281 ctggacaggt tctagcggat ctgcttcata ttcctttgat tcctcagact cgcttggaaa
     8341 aaatttatcg acaaagcgaa gaatcaacca tcgtcaccct agctagtcag attcgacagg
     8401 gcatcttgcc agctgatttc acccaaaaaa aagctgaccg ttcctacttt gaaattgcta
     8461 gtggccatat tcctgccact attgaaaaaa tcttagatgc cgccctcaga aatggtattc
     8521 ctgcccgtga tatccaagtt ctggctccta tgtaccgagg gacggcaggg attgatgcta
     8581 tcaatcagct catgcaagac ctgctcaatc ccccacaaaa agatcaactc agttttgaag
     8641 ctcctcagtg ccactatcgt aagggagaca aggtcattca tttggttaac gatgctgaaa
     8701 tcaatgtctt taatggggat ttaggagcta tcacagacct gattcctggt aaatacaccg
     8761 aatcgaaaca agacgagatt gtcattgatt ttgatggtaa tgaagtctct tacccccgta
     8821 acgaatggta caagattcgc ttggcctatg ccatgagtat tcataagtct cagggaagtg
     8881 agttcccagt tgtcatccta ccgattacta gtgctagccg acgtatgctg gagcgaaatc
     8941 tcatctacac agccattaca cgtgccaaaa gcaaactcat cttactaggc gaattacaag
     9001 ccttcgacta tgctacccaa cacatcggaa ctgcccgaaa aacctatctg attgaacgct
     9061 tcagtgatct actggagaat gttgaagaaa agcaacaagc tgtctctgaa acagtcacat
     9121 caagtgcctc tgaacaatcc tacatcctaa ccgaagaaaa ctgggacagc attccagcca
     9181 tgattgggat tacagacaca gacctcaaag agatttttgg aaaatagtac atcagaaaac
     9241 ccaccaattc aggtgggttt tctgtttatt aagattattt tactgttgct gtgcttgtga
     9301 tcaattcaac agcttttttg atagtgatat catgtttcaa catgtcagct gaaagcaagt
     9361 tttgaacttg tgcaacttcc atgttgtagt ctgctgccaa ttgctcaact tctttttgga
     9421 tttcttcttc tgaagcatca aatccttcag ctttggcaac tgcttcgata acaaggttag
     9481 tcttagtacg tgactcagct tctgcttggt attggttgtg aaggtcttct tgagtagttc
     9541 cagtgatttg gaagtacatg tcagggttga tcccttgacg ttgcaagttc ccaaggaatt
     9601 catttactga acggtgaact tcttcgtgga tcatttcttc tggaagttct acgatttcag
     9661 cattttctac agctgtatca attgctgcac cttcaactgc atctttgtaa gcttcttctt
     9721 tagcagtagc caattctttg cggtattttt ctttcaagtc agcaagtgtt tcaacttctt
     9781 catcaatgtc ttttgcaagt tcatcgtcaa gagctggaac ttctttagct tttacttcgt
     9841 ggatagttgt cacgaattta gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg
     9901 ggaatgttac gataacatca acggtttcgc cagctgagtg acctaccaat tggtcttcga
     9961 aaccagggat gaattgacct gaaccaagtc caagtgagaa gttttcacct tttccaccgt
    10021 caaattcaac accgtcgata gaaccaacga agtcgatcac aacagtgtcg ccgttttcag
    10081 cagcagcttc cttgataacc aattcagcca ggttgttgcg ttcgcgttcg atacgctctt
    10141 caacatcagc gtcagttact tctttttcta catcaactga tacttcaagg tttttgtagt
    10201 cacccaattt tacttcaggt tttgtaacga cttcagcagc gataacccag tcttgacctt
    10261 tttccattga agttacgtca atttttggtt gggcaaccac ttcaagacca gcttctttta
    10321 cagctgcttc ataagcgttt ggcaaaagag cgttcattgc atcttgataa agagcttctt
    10381 caccaaattt ttggtcgaag atagggcgtg gaaggtgacc tttacggaaa cctggaacat
    10441 taagagattt cttcactgac ttgaagacac ggtccaattc tggtttgatt tggtcttgag
    10501 agatagtgaa agtcaagaca ccacggtttg tttctttgtt ttcaaatgat acagacattc
    10561 tgtcatttct ccttaaaatt ttttaaatac agtctattat aacattttct ataaataaaa
    10621 tctagactaa tgcagccaat tatagacaac aagccttttt cctaactaaa acactcataa
    10681 agtatagaca attttagact actttggata tcttttggtt agtcattttc caaaactaat
    10741 ttatctaatt tttcagttat atagta
//
LOCUS       DAVLFB010000071        10736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLFB010000071 DAVLFB010000000
VERSION     DAVLFB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.071"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(95..1519)
                     /locus_tag="VXD58_001554"
     CDS             complement(95..1519)
                     /locus_tag="VXD58_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV0676224.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VXD58_001555"
     CDS             complement(2064..4343)
                     /gene="pulA"
                     /locus_tag="VXD58_001555"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV0676225.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VXD58_001556"
     CDS             complement(4455..6413)
                     /gene="ligA"
                     /locus_tag="VXD58_001556"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV0676226.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6506..7684)
                     /locus_tag="VXD58_001557"
     CDS             complement(6506..7684)
                     /locus_tag="VXD58_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV0676227.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7764..8627)
                     /locus_tag="VXD58_001558"
     CDS             complement(7764..8627)
                     /locus_tag="VXD58_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV0676228.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8630..10531)
                     /locus_tag="VXD58_001559"
     CDS             complement(8630..10531)
                     /locus_tag="VXD58_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0676229.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3231 a         2364 c         1907 g         3234 t
ORIGIN      
        1 attataatac caaaaagttc gtattttttc tataattatt attattttat agcaaaaaaa
       61 taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa acaatggatt
      121 tgacatttgt catcgcttca atgctatatt taattccttg cactccagct ccagaacctt
      181 tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta atgtggactg
      241 tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt gtaaagactg
      301 atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct acactagcca
      361 cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc atatcttttg
      421 taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt ggtgtaagag
      481 cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg gctgaagcat
      541 tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt aatttagaaa
      601 cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca atgacacgtt
      661 taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt tgcttggcag
      721 catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca agttccaaca
      781 tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt gaacctgtaa
      841 agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct gaaccacgac
      901 ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct ttagccaaca
      961 agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc cctgcaatca
     1021 aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga gcaatcgcta
     1081 gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt gctgcctcaa
     1141 aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca gcataacgaa
     1201 tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt gctacttctt
     1261 tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct gttttatgta
     1321 aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct gcacgcgcag
     1381 cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc aattcttctt
     1441 gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt ccatttacta
     1501 aattctgata tcttgtcaaa ttcttgtcct cctgaaaatg attaagatat attttaccaa
     1561 attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca caaacttgat
     1621 ttttcaaagt atactcaata aaaataaaaa aaatccacta cgtcaaggcg aggctaatgt
     1681 ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa taaaaaaact
     1741 gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa acaagggtgt
     1801 gctactccaa tttcagccta ctataactgt catagattgc tgaaacaaag tctaggtaaa
     1861 agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag gaggtcttgt
     1921 tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg ttctttctat
     1981 tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat ttgtctgtgt
     2041 aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact aatatcattt
     2101 aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt tcctgcattt
     2161 tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc tttgccgtga
     2221 acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac atgatggtaa
     2281 atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg gatgacttga
     2341 cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac ttggttcaca
     2401 ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt caactcacca
     2461 ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc cataaaggcc
     2521 atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat gatttgctct
     2581 gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt gtcatgggct
     2641 tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg acttcctagg
     2701 attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga cttgatagca
     2761 ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa accaatattt
     2821 ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc tgttcccata
     2881 tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct ttgacgaatc
     2941 atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc gtcaatatta
     3001 tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat ttcgtgttca
     3061 ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg attcatacga
     3121 taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa ggtatggtta
     3181 tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac catcaccttc
     3241 aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt ttctggcgcg
     3301 ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc atcgtattct
     3361 ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt cttgatgtaa
     3421 tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc acccaagaaa
     3481 gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc acgaatatgc
     3541 atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc cgaaccgtgc
     3601 ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga acgtttgcca
     3661 tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg agggaattgg
     3721 acttgatact gataagcctt acctaccaaa tcttcctcaa catccaaact ccagacaccg
     3781 attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc aaaagtcttc
     3841 caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc tgtcgctgta
     3901 ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa ttctccttgg
     3961 taaccccaat gatggtcaaa actagcactg ttaatggcct tatcaaaggc aaaaggattt
     4021 tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat cctatcatcg
     4081 ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat agaatattct
     4141 ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg tgaacttggg
     4201 tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa atcaattcgt
     4261 tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg atggtaatga
     4321 atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt ctatttcact
     4381 aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc taaactctgt
     4441 ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg gataccaagt
     4501 tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag gtcggtcttt
     4561 ttagaaatac tacctgtcac tttggcaccc agactttcga gtttactttt agcttctgag
     4621 cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa ggccgcatcc
     4681 gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa ttctctgagc
     4741 agaatttcag agccttctgt cgcaaaataa gtctgaagac ttttggcaat cacgccacct
     4801 agactttcaa tactagccac ttcctctgaa tctgcctgag acagattttc aattgaatgg
     4861 aaatattgaa gtaaaagctg actaaccttg cttccgacat gacgaattcc caaaccaaat
     4921 aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag tttagcagcg
     4981 gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg ataaatatcc
     5041 gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg accaaggcct
     5101 gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat ttgagcaggg
     5161 caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa gtcagagtta
     5221 caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt ggactctacc
     5281 acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt atcgtctttt
     5341 cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct aacagtcgta
     5401 ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt acggccaact
     5461 gtccagtcaa ctgataagag ttgagcttct ttttcttcgg cagggaactt gtaggctact
     5521 gcccactttg gagccttaac tgtaaaacca agttcttctt gacttgctag gtcgttgacc
     5581 ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc tacttcttgg
     5641 ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt gaccacaaaa
     5701 cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga agggctggct
     5761 tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc caactgacgc
     5821 agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt ttcttggcgc
     5881 acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg aactgtgata
     5941 tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt ttctgtgata
     6001 ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc cttttcataa
     6061 gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg atgagccact
     6121 tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc atcctgcaaa
     6181 ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt gccaccaaca
     6241 cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa ctcgaccaac
     6301 tcacggtaaa ggcggtcata ctcactgtct gcaaccgagg gattatcgct ggtatagtac
     6361 tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt cataagacca
     6421 ttttaccata aaacaagccc tcctcacaaa cgagaagggc ggaaaaaaca cttagtttga
     6481 aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt cgaacataaa
     6541 taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa gaagagagat
     6601 gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga atagctaagg
     6661 taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt tgtacttgag
     6721 taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt ccagaaaagg
     6781 aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc aaagccagta
     6841 aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc ccaatgatgg
     6901 aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg taaagctgat
     6961 tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg gaagctacca
     7021 gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc ttgatatcta
     7081 caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc tcttttggaa
     7141 ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt agcgtcatat
     7201 tgagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca cctacaacct
     7261 gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc accacttcag
     7321 ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc acaatgttag
     7381 caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata gccagacaaa
     7441 gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa cggtctgaaa
     7501 taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc tgatacattc
     7561 ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt ccataatcat
     7621 agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact gcatagcgat
     7681 tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa ggagcttttt
     7741 attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg ttcctgataa
     7801 taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctt ttgcatctgc
     7861 ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa ataatggaaa
     7921 acattacgct cataaagctt aatacctttg tcaataatct tctctgtata agcctcaaaa
     7981 tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt gagggccaaa
     8041 atcaaaacta atctcttatg gcgactaatc tcttggtaaa attcctccct ctccataact
     8101 tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt accgaaaaga
     8161 atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac cttatccaaa
     8221 tcatcctgct tcatatcata agtcgcatct ctttgacaaa tcagaccttg tagcaaaatc
     8281 cagttcagct caaaataaag ggaagtcgtc gaactcttag acttttcaag ttgttctctt
     8341 tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat ctgtgccatc
     8401 atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt ttcgattgtt
     8461 acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga ctccccaagc
     8521 tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt taaagaataa
     8581 tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt tacacctgat
     8641 ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt ttgtccagct
     8701 cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt tcagaaatgg
     8761 cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt tgtcgcatga
     8821 gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact ggacttgctt
     8881 cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct gttgctttct
     8941 tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca gacaattcca
     9001 aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaaa agcaaggtac
     9061 catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc aagtggttgg
     9121 tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct aaaagcaaac
     9181 gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct cctgagaaaa
     9241 ggaaggcccc aagacggttg cggatttcaa cttctggtgt cagtttgaaa tcattccaga
     9301 gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca tagtaaccaa
     9361 cctcaacatt agcgccaaag cgcttttcac ctttgataaa aggaatctgg tccacaatag
     9421 acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca gcattcatct
     9481 tacgaagatc taggttgata ggttgtgaca aaacttcccc gtcataacca atagcagtat
     9541 tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc atgttggctg
     9601 ctttcttgcc agcttcaggc ttgtccaaac gctccatttt ttctagttgt ttacggcgag
     9661 attgagcacg tttggtcgtt gaagcacgaa ctagattgcg attgacaaag tcttccagag
     9721 cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtagctagc ttttgctcct
     9781 tcaactcaac aaaacgagag taattcccca catagcgatc caaggaatac ttggtcaaat
     9841 ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg ataatgaggg
     9901 caccgctata gtttaccaag taattctcta gccaggagat ggtttcaata tccaagtggt
     9961 tagttggctc gtccaagacc aagagattgg gcttttcaag gagcatttta gctagagcca
    10021 aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata gactcgtcaa
    10081 acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca cctgcttggc
    10141 gaaaattttc agataagcgg tcataatctg acatcagttt atccaaatcc tcaccagact
    10201 tttcacccat ctccagctcc atctgacgca gttgtttatc tgtccgacgt aagtcatcaa
    10261 agacatgaag catctcatca tagatggtat tttcagactc aaaacggcta tcttgagcta
    10321 ggtaagacag agaaatatct tttttcttat tgatttctcc actagttggc tcctcttctc
    10381 caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca agggcaatcc
    10441 gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct gcaaaagaac
    10501 gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt tccctaaggc
    10561 attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa actaaattat
    10621 gtatatttta tattagattc cttcactatc ttgttggatt ttctaaccag ctaatcttgt
    10681 ttcaaatagt tatcgcacaa gcctattatt taattctttt cattatttac gtacgc
//
LOCUS       DAVLFB010000072        10727 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLFB010000072 DAVLFB010000000
VERSION     DAVLFB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.072"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(72..1286)
                     /locus_tag="VXD58_001560"
     CDS             complement(72..1286)
                     /locus_tag="VXD58_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0676230.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1442..1736)
                     /locus_tag="VXD58_001561"
                     /pseudo
     CDS             complement(1442..1736)
                     /locus_tag="VXD58_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1898..2350
                     /locus_tag="VXD58_001562"
     CDS             1898..2350
                     /locus_tag="VXD58_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV0676231.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2385..3773)
                     /locus_tag="VXD58_001563"
     CDS             complement(2385..3773)
                     /locus_tag="VXD58_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV0676232.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3839..4801
                     /locus_tag="VXD58_001564"
     CDS             3839..4801
                     /locus_tag="VXD58_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV0676233.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(5007..6017)
                     /gene="ccpA"
                     /locus_tag="VXD58_001565"
     CDS             complement(5007..6017)
                     /gene="ccpA"
                     /locus_tag="VXD58_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV0676234.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLVKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6441..7040)
                     /locus_tag="VXD58_001566"
     CDS             complement(6441..7040)
                     /locus_tag="VXD58_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0676235.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7041..8138)
                     /locus_tag="VXD58_001567"
     CDS             complement(7041..8138)
                     /locus_tag="VXD58_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0676236.1"
                     /translation="MLERLKSIDYMYWASLIFMIFPILPVVTGEIPSWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERAEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVSPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8138..8875)
                     /locus_tag="VXD58_001568"
     CDS             complement(8138..8875)
                     /locus_tag="VXD58_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676237.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAMFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLGSWIGAAFLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYILVFSAVLFGLTIYLQGHKENA"
     gene            complement(8877..9761)
                     /locus_tag="VXD58_001569"
     CDS             complement(8877..9761)
                     /locus_tag="VXD58_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676238.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9748..9942)
                     /locus_tag="VXD58_001570"
     CDS             complement(9748..9942)
                     /locus_tag="VXD58_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676239.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(9939..10070)
                     /locus_tag="VXD58_001571"
     CDS             complement(9939..10070)
                     /locus_tag="VXD58_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEV0676240.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKEFEE"
BASE COUNT         3272 a         2448 c         1934 g         3073 t
ORIGIN      
        1 cttggactac aagtcgccaa gtgaatttct tttgggtggc taacttcaac ttgaaatttg
       61 ggatgcaagc cctatctacg atactgtttc aagaaacgag tcatcttttc ttggatttgg
      121 gctaactcat caacacgagg aaggtaaacg atacggaagt ggtctggttc ctgccagtta
      181 aagcctcgac catgaaccaa gagaaccttt tcctgcttca agaaatcaag gacaaactgc
      241 tcatcatcat cgatacggta catattgcgg tcgatttttg ggaagatata gagccccgcc
      301 ttgggtttaa cggcagacaa acctggaata tcttgaatgg cattatagat gaaatttctt
      361 tgctcgtaga ttcgtccacc aggaagaagc aattcatcga ctgattggtg accccccaag
      421 gaagtttgta cgacttgttg ggccaaaacg ttagagcaaa ggcgcatatt ggacagcata
      481 ttgagccctt cgatatagcc cttaacatga gtcttagggc cagacaagac catccaaccc
      541 acacggaaac ctgctatgcg gtgggatttt gacagaccat tcatgctgac acagaagaca
      601 tctggtgcca agctcgccac aggcgtatgc acatgtccgt ccattaccat gcggtcatag
      661 atttcatccg caaagataat caaatcgttt tgacgggcaa tctcaataat ctccagcaag
      721 agttccttag gataaagggc tccagttggg ttatttggat tgataaggac aattgccttg
      781 gtattggaag tgatttttga cttgatatcg tcaatatctg ggtaccattc cacagcttca
      841 tcacagatat agtgaacggc atttcctcca gctaggctga cagccgccgt ccatagagga
      901 tagtctggca ttggcactaa gacctcatcc ccattgtcca aaagcccctg catggacatg
      961 acaatcaact cactgacacc atttccaagg taaatatcat caatatctac gtttgggaat
     1021 ttcttcagct ggcaatactg catgatggcc ttacgggctg agaaaatccc tttggagtca
     1081 gaatagcctt cactatcacg cgcattcata atcaagtcat gaatgacctc gtctggcgct
     1141 gtaaagccaa attctgctgg atttcctgta ttcagacgta aaatcttttc tccattcgct
     1201 cgcatccgca tggcttcttc caatacagga ccacggatat cataagcaac atgctctaac
     1261 ttactagact tgttatattc tttcatcttg tttcctcaat tcatcaggat agtaacatta
     1321 taccactaga aagagaatgc gacaagttta ctgaaatgtg ttactagttt cgtatagaag
     1381 aaaaaccttg ctaaaggcga ggttaagaac gtagagatag caggtatttt gcaaagacac
     1441 atcacatacc aaaagataaa cctagaaaaa agcaagccta gaaaggagtc ataaagaaga
     1501 ttaaacatga ggaaaagccc ccatatcatc aagtaatccc aacgcccact tcgttttgca
     1561 actagaaata agattagatg gtagagtaca tgaaaaacta aaaatccaat aaaacttgga
     1621 taaagaagcg gtaccagact ctctaattga cagatcatga tcacttctac caagactgaa
     1681 actaggataa ttccataata aaggaaattt gatttttgaa aagagttttt catcattctc
     1741 ccttccttca cttctttcct gctatccttt cttttatttt atcatatatt tatatagagt
     1801 ttaagcccta atattctttt gaaaaatact ttttttgctt tacttctata gtgaaaaggt
     1861 ttacaattat agtaagaaaa tttcaggagg tttcattatg gcacaacgtt accaaaatat
     1921 catggtcgca atcgatggtt ctaaggaagc ggacttggct tttgtcaagg gagttcattc
     1981 tgctctacga aacgacgcta aactcaccat cgcacatgtc attgacacac gcgctctcca
     2041 aagcgtatcc acctttgatg ctgaagttta cgaagaactc caagtcgacg ctgaaagtct
     2101 gatgaaagag tacgaaaaac gtgctaaaga tgctggggta gcagatgttc atatcgtcat
     2161 tgaaatggga aatccaaaga ccctgctagc acgtactatt ccagatgccg aggaagtgga
     2221 cctcatcctc gttggcgcaa ctggtctcaa cgcctttgaa cgcctcttgg tcggctcttc
     2281 atctgaatac atactccgcc atgctaaggt cgatttgctg gttgtgagag aacaagaaaa
     2341 aaccttataa tcacaaagaa aaggagcccc tagctccttt ttgtttacga tttatttctc
     2401 tctttatggc gttcgtaagc cttgagctgg cgctgcagtt cctttttaat agcaggttct
     2461 ggagcatatt tttcttccca attatctggt tttaagattt tatgggtcac tggatcaaaa
     2521 tgagccttgc catctggaaa aattttcccc atattggcct gatggacaat atcaaaaata
     2581 cgttctgggt ccacccccat caagacaaaa ctgccgtagg tgaagtaaag cgtgtcaatc
     2641 aaggcatcca cttgccctat caaatcttgc tgagcaggtg tcttcttggc tactttacct
     2701 gctgccttat caagggcctg atgaagttgc gatacagctt gaccaaaatc ttcttcagaa
     2761 ggactggctg atcgaacaaa ctccaccaat tcttctattt taaaaccagc cctatgggtt
     2821 gcaccctcta aatcccaagc tcgaggttct tcttgggttc gttcatccat catgtggtgg
     2881 aaggtcttga ccttattgaa atgatagtca cggctgacaa aaactttttc tgaagacaaa
     2941 acaccaaaat gttccaaggc cttgtggatc ccatcttgtt gattgctagt ggtaatgtgc
     3001 ttggcagctt ccttgacact gctactacca tttcccatag cgaccgacat accgacgcca
     3061 gccagcattt caaggtcatt atctgagtca ccaaaagcca tgacttggtt gaggtcaaag
     3121 ccatattctt tcccaactcg gcgaatgcct tctaatttag aatttccctg attgatgaca
     3181 tccgatgcaa aaggattgct acgtgtcaat ttcaagtctt caaaatcagc tgccgccttc
     3241 tcagattctt ctggtgtcat cagcatcaaa acttggtaaa taggttgatt catcaggtga
     3301 agcaggtcct cttccttttg gggaacaacc ttgctgacca tgcgattaaa agactgactc
     3361 accgtccgag ttaaaacaga gggaacgaag cgactaattc gttgggaaaa agaacccaga
     3421 ccaaaggaca tgattttaga acccaacatg gcatccttgg tccctaaggc aatctccgtg
     3481 ccctcttttt tagcatagct aattagatgg cgcaaatgta acttggaaat agggctcgtg
     3541 aacaagactc tgtctttact aaagatatac tggccattat aggttaccgc aaaatccaga
     3601 tccaaatcgt ccatcaattc cttaacaaaa aaaggtcctc gccctgtcgc tacgccaact
     3661 agtacccctt gttctttgac aatcttaatc gcatccttag tggatttcaa aacactcttg
     3721 cgattgttga ccaaggttcc atcgatatca aaaaaaacag ctttgacttc catcctatcc
     3781 caatctcccc ttttgtgata caatgattat accacatttc agaaagagtg agtaaatcat
     3841 gcctaagaaa atccttgttt tacatacggg tggaactatt tccatgcagg ccgatgcttc
     3901 tggcgctgtt gtgacgagtt cagataatcc catgaaccat gtgtccaacc cacttgaagg
     3961 aatccaagtc cacgccttgg acttttttaa ccttccaagt ccccatatca aacccaaaca
     4021 tatgctggtc ctctaccaga aaattaaaga ggaagcagat aactacgatg gagtggtgat
     4081 cacacacgga accgatactt tagaggaaac agcctatttc cttgatacca tggaagttcc
     4141 ccatatgcct atcgttctaa caggagccat gcgtagctcc aatgaactcg gtagtgatgg
     4201 tgtttataat tacctaagtg ctttacgagt ggccagcgat gacagggctg ctgacaaagg
     4261 agttttggtc gttatgaacg atgaaatcca cgctgccaag tatgtcacca aaacacatac
     4321 gactaatgtc agcaccttcc agactccaac acatggcccc cttggtctca tcatgaaaca
     4381 ggaaatcctc tacttcaaaa cagctgaacc tcgtgttcgc tttgaccttg atcacataca
     4441 aggtttagtc cctatcatct cggcttatgc tggtatgaca gatgagctga ttgatatgct
     4501 ggatttagaa cacttggacg gtttgattat ccaagccttc ggagctggta atattcccaa
     4561 agaaacggct caaaaattag aaagccttct gcaaaaagga attccagtcg ctctggtatc
     4621 acgatgcttt aacggtattg ccgagcctgt ttatgcctac cagggtgggg gcgtacagtt
     4681 gcaaaaagca ggcgttttct ttgttaaaga actcaacgcc caaaaagctc gcttgaaact
     4741 cctcatcgcc ctcaatgccg gactaacagg acaggctttg aaagactata tggaaggcta
     4801 atactcttcg aaaatctctg caaaccacgt cacgtcgcct taccgtatgt atggttactg
     4861 acttcgtcag tttcatctac aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc
     4921 ctagtccgct ctatggtatt cattaagtca acatctcttg tttaagagca caaaatcagg
     4981 aaatcttctc gattccctga ttttttctat ttacgttttc gtgttgagct acgttctgtc
     5041 aaaccatgag gtaagagaac ttcacgttct tccaactctt ccttatgcat aatcttggtc
     5101 aacatacgca tactaatggc accaaggtca taaagaggtt gggcaatcgt tgtcaagttt
     5161 ggacgggtaa agcgtgagat ttgtgaatca tcactagtaa taatttcaaa atcttctggc
     5221 acagaaacac ccttatcagc caaaccgttc aagactcctg ctgccaactc atcacctgtc
     5281 acaactgctg cagttgcatt tgatgaaatc aaacgctctg ctaaggcgta accatcatca
     5341 tagctatatt tagattcaaa taccaaaccc tcactataag tgattcctgc ttttttcaag
     5401 gtttccttgt agccaactaa acgaacctta ccattgatgt catccactag cggaccgcta
     5461 acgaaagcaa tacgctcatt ttctttaaca aggtaactca ctgcatcaat tgttgcttgc
     5521 ttatagtcaa tattgacact tggcaactgg tgctcaacat cgacagttcc tgcgagaaca
     5581 atcggagtac gtgaacgcga aaattctgag cgaattttat ctgtcaagtg ataacccata
     5641 tagataatgc catctacctg ctttgaaaag agggtattga caacagaaac ttctttctcg
     5701 ttatcttcat cgctattagc taggacaata ttgtacttgt acatttctgc aatatcatca
     5761 atccccttag ccaaactcga aaaataacca ttggtaatat ttggaatcac gacaccgaca
     5821 gtggttgtct ttttacttgc aagaccacgc gcaactgcat ttggacgata atccaaacga
     5881 tcaattacct ctagcacttt tttacgggta ttctctttta catttttatt gccattgacc
     5941 acacggctga ccgtcgccat ggaaacacct gcttcacgag cgacatcata aatggttact
     6001 gtatcatctg cattcattcc ttttcctgtc ctttctatct cacacattct tttacaagta
     6061 gaggtgctga ttgaagctct atatctactt acaaaagtga agatgtgaaa atttcgtttt
     6121 catatttcta cttattccat tctatcacta attgtaaaca ctttcaagtg ttttttgaag
     6181 attgattgaa aaaatttcat agaaaaccta ggtttagctc cttgctacca ccttagacta
     6241 aacaaaaagg aggaaactaa gccctcctaa agttatagta aaatgaaata agaacaggat
     6301 aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat
     6361 tctagtttca atctactata ggtattgttc cattcactac cgtcaatttt agcacatagt
     6421 cttcataaaa tattatatca tcataaccaa ccagattctt ttgcgatatt agctgcctct
     6481 gttcgattac cagcatccag ttttgaaaga atattggtga catagtttcg aatagttccg
     6541 tttgagaggt aaagctgatc tgcgatttct tggttggata agccccgggc gattcccttt
     6601 aagacagcaa tctcttgttc tgtcaacgga ttgggacggg tcatcaccac ttccatcaat
     6661 tcaggcgaat actccttgcg tccttcgagg acggtgtgca aggtttgcat gaggtctgca
     6721 atgcttcgtt cctttaatac ataagcatct actccagcct tgaccgcacg ttcaaaatac
     6781 cctgggcgct tgaaggtcgt cactacaacc acctttgttt cgagcttttc tgctcgtatc
     6841 cactccaaga cttcgagtcc tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct
     6901 acagactcct tttctaatag ttgaattgct tcttgaccat tcttggcttg taagacagac
     6961 tctacatctg cttgaaaggc aagcaactgg cacatggcat ctcgcaacat actttgatct
     7021 tctgcgacta atactttcat ctactttctc tccttataag gtagtcgaac ttgcacttcc
     7081 gttggatgtt tctgactgat tacacttact tctcctgaaa atggagaaac acgatctcgg
     7141 actgtatgga gctcatctcc ttttagaaaa gcaaagccgc agccatcatc acttactgtt
     7201 aaaatgagtt ctttctctgc ccgttctaat ttcaggtagg ccttcgacgc tttggcatgt
     7261 ttgataatat tggtcaccaa ctcaagcaaa atcatggaag ccgttgactc caattcttga
     7321 gtaagactag cagtatctag ttggttatcc gtttccacct caattccagc aatttctaac
     7381 atctttttca cagtctctag ttcggatgtc aaagttctag acttaagatt ttccacaatg
     7441 gttcgcactt cattcatgga atccttactg atctggtgaa tttcttttaa ttccttttcc
     7501 acctgtggat aagcctccat ctgaaataac tgcaaggcta aatctgtctt gacactcaac
     7561 atagcaaagg tatgtcccaa gctatcatgc aaatcctgac cgatacgact gcgttcattt
     7621 tcagcaagca atagatttat ctgggcattt tgcttgacct gagcttcttt caaatcctcc
     7681 acaatacgaa tccgaaccaa gccaaaagtc attaaatcga caaaagcaag aattacaagt
     7741 agatagaata gaaactcaac ttcgattctc tgaaaaatca acagttggcc cacaacaagg
     7801 acttgagcaa gaagaaaagt ccagacatgt aaagacttta aaccacctac gctgaaatga
     7861 taacttaaga gattggatag gaaaaagaaa aaccagatat aattaacagc aacaaaggca
     7921 gtattcccaa ctacataagt cagcatgagg ccccaatata gccaagatag gcgctggctc
     7981 ttagttgtta aaacacccaa atacgccact acaaatagaa tatcaatcaa taaatgccag
     8041 ctaggaatct ccccagtcac tacaggtagg atggggaaaa tcataaaaat taaactggcc
     8101 caatacatat agtctatgct ttttagtctt tcaagcatta agcattctcc ttatgacctt
     8161 gaagataaat ggtcaaacca aacaaaactg ctgaaaaaac aagtatataa actgtggctg
     8221 atagattgat gccaccctcg tttaagaagg ttttgagcaa ctccatcaac tgataagttg
     8281 gtaggcactt cccgattgct tgcatccagt ctggaaataa agagatgggc atccagagtc
     8341 cgcctaaaac agccaagcct agatagagaa gattgcccac gacagacatc aactgactag
     8401 aaggcaagag tgtcaaggtc aagccaagcg ctacaaaggc aacacttcct actattagca
     8461 agaacgcagc cccaatccag cttcctagag gcatatccac acctcttaca aaatgcccaa
     8521 ctgagaaaac aaccagaatt gataccaaat aatcaaccat catacttgtt atctttgata
     8581 gataatattc taccatattt acaggggtat ggcgcaatgt tttctgccag ttgttgattt
     8641 tatcagtatg taagacagct gggaatgaaa acatagctgt cgacatcatg gaaaaggctg
     8701 tcatggagat gaggtaatca cgcataaaat tcgcgagttc acctggtgtg tcctgataga
     8761 taccagaaaa aaataaatag aaagccgtcg gcatccctac cgacaataga taatagatta
     8821 attgtcgttt ggtcaataaa aattctatct tactaagtgc tatccatcgt ttcatcttag
     8881 ttatctccct tttgtgtttc ttcaaagatt gtattcaaca aactacgatt attaacttcg
     8941 atttcttgca tcctacatcc tgcttgggct aatagttccc agaaagcatc tgcttctcga
     9001 gtgactactt gtagagcatc ctgtttttgt gaccagtttt caaccaagtt agactgctca
     9061 atgacttcct tgtatgccag aggaaggata aaatgctttt caattccctc actacgcata
     9121 gctagaggcg tcgtatcacg aatcaactct cccttattta aaaccaaaat ccggtcagcc
     9181 gtatgctcta cctcttcaat ataatgagac gaatagagaa tcgtgactcc ttgcgctttt
     9241 aggtcccgaa cgatttccca aaagcgttga cgagttgaag tatccatggc agcagttggt
     9301 tcatctaaaa agacaagctt tggtcgccca atcaaggtca agacaaaaga gaagagacgc
     9361 ttttgcccgc ctgacaattt ttctgcgaat tgctcttttt gttgctggtc aaactgcaat
     9421 agttgatcga tttcctgatc gctcaaggaa tttggataga tacgttgaaa gaaagcaatc
     9481 aactctttga cctttaattt ctgaacgatg acattttctt gaggcagata acctctaata
     9541 tagtctaact gagaactcgt cactgacaag ccttggatgg atacttgacc gcttgtgacc
     9601 agtttatctc caagcagaca gtccaagagt gtggtcttcc cagcaccatt gggcccaatc
     9661 aaggcgacgc attcaccttc agctacctca aaggaaatac ccttcaaaat agccttgccc
     9721 ttgatgtttt tatttaggct ttctacctta atcatattca tgatattctc ctttcaacca
     9781 ctccattctc ataaggaaaa cgacgaaaat cataaatcca aaccccaaag caccacgaat
     9841 gaattggcga agcaaggttt ggtcaaacca acctgtaaac atttccacta accataccaa
     9901 gagtgacagg ccgataaaga aatagatgat ccctctcttc attcctcaaa ctcctttttc
     9961 acatctccga ctaatttcaa accttctcta acaagccaag acatcattcc aaagccagca
    10021 aagagctccc aaggaaaatg atagaaactc tcatccaatc ccgaaaacat gagttaggtc
    10081 ataactcctg ctactactaa actcactgcg ataatcattt tatttctcat ctcttcttcc
    10141 tccatttcat actacaatta tagtcttttg aaatcagagg agacagaagc ttctgtcact
    10201 agaaaatatg acaaatgtca taaaaaattc tgttcaaaac aagcaagata cactatacaa
    10261 taaaacacaa tcagaaaaat ctaaggcaac ttcctcaaaa gagatatcaa acccaattca
    10321 caccataatg taaactaata cttatttaaa atcaaaaaga gtagaaattt ttatcagaca
    10381 aacacatata tagtgtattg aatctataac agtagggctt aaatactaac atatttctat
    10441 aaattaattt aactttcctg atagagctgt tcatatctta tttcaattct ctatattata
    10501 cgttgaacaa aacccttcta ttgctttctt aaaaatttat aagagttata aaatctgtta
    10561 aatttcaatg tgtataccta aactacggta tttattgaaa agactgggga caaaaagtat
    10621 acgctgccaa aatgaattac tgaaaatcaa aaaagagaga accaaactga ttccctctta
    10681 atgtatataa tatctagttt taaaaataca cactcacata tctctgt
//
LOCUS       DAVLFB010000073        10678 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLFB010000073 DAVLFB010000000
VERSION     DAVLFB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.073"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            85..582
                     /locus_tag="VXD58_001572"
     CDS             85..582
                     /locus_tag="VXD58_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV0676241.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            582..2237
                     /gene="dnaX"
                     /locus_tag="VXD58_001573"
     CDS             582..2237
                     /gene="dnaX"
                     /locus_tag="VXD58_001573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV0676242.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2265..2459
                     /locus_tag="VXD58_001574"
     CDS             2265..2459
                     /locus_tag="VXD58_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV0676243.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2609..3379
                     /gene="sufC"
                     /locus_tag="VXD58_001575"
     CDS             2609..3379
                     /gene="sufC"
                     /locus_tag="VXD58_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV0676244.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEGDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3407..4669
                     /gene="sufD"
                     /locus_tag="VXD58_001576"
     CDS             3407..4669
                     /gene="sufD"
                     /locus_tag="VXD58_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV0676245.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4680..5906
                     /locus_tag="VXD58_001577"
     CDS             4680..5906
                     /locus_tag="VXD58_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0676246.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5893..6333
                     /locus_tag="VXD58_001578"
     CDS             5893..6333
                     /locus_tag="VXD58_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV0676247.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6387..7799
                     /gene="sufB"
                     /locus_tag="VXD58_001579"
     CDS             6387..7799
                     /gene="sufB"
                     /locus_tag="VXD58_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV0676248.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(7998..9239)
                     /locus_tag="VXD58_001580"
     CDS             complement(7998..9239)
                     /locus_tag="VXD58_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV0676249.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9342..10451
                     /locus_tag="VXD58_001581"
     CDS             9342..10451
                     /locus_tag="VXD58_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV0676250.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3165 a         1950 c         2323 g         3240 t
ORIGIN      
        1 aaatattata gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg
       61 ctcctttttt gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg
      121 ttaaatgaag aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc
      181 aacgccagtg ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg
      241 ttcgatggaa aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt
      301 gcactaggca agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga
      361 gatgtgacga cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg
      421 gtgccgatga tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt
      481 gaggattacg atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa
      541 aagacaacat gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc
      601 gaaaatatag aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc
      661 ttaaacaagc ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg
      721 gaacgggaaa aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag
      781 tgggtggcga accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag
      841 aagatgtcat tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc
      901 gtgataaatc tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg
      961 ttcacatgct gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac
     1021 agaatgtagt ctttattttg gccactactg aattgcacaa gattcctgct actattctat
     1081 cccgtgtgca acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc
     1141 actatatctt agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca
     1201 ggcgggcgga aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga
     1261 cacagggaaa tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat
     1321 cagccttgga tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt
     1381 gcttgaatct tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc
     1441 actatttaag agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag
     1501 tctttgtaga aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa
     1561 cagtgagttt agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga
     1621 ccgtccgttt ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa
     1681 ttgctacgct gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg
     1741 ttcctaaaca agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg
     1801 tcgatcgcaa taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac
     1861 gtcaaaatct aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc
     1921 cggacaaggc tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc
     1981 ttgcttttga gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca
     2041 tgtttggtaa tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca
     2101 tggaggaatg gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg
     2161 aaaaagaagt agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga
     2221 aggtagagga agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat
     2281 ttattattat ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga
     2341 ctggccgcta tattatggca gccttttggg caattttact ctttagaaat ttccgagtca
     2401 gttatgtgat gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc
     2461 cctcagcttc cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta
     2521 tatttataga atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt
     2581 aaaagttttg aaaaagaaag gaactatcat gtcagtatta gagattaaag atcttcacgt
     2641 tgagattgaa ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat
     2701 tgccgctatc atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg
     2761 aaatccaaac tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga
     2821 gttggaagtg gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga
     2881 aatccctgga attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaagg
     2941 tgatgagaag atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct
     3001 caacatgaaa gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa
     3061 aaaacgcaat gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga
     3121 gattgactca ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat
     3181 gcgtggtgaa ggttttggtg ctatgatcat cactcactac caacgtcttt tgaactatat
     3241 cacacctgat gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga
     3301 attggctgcg cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga
     3361 ctacaaggaa gaattgtaat ttcctcgtat cttttaggag aagtaaatga ctagagaaaa
     3421 tattaaactt ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa
     3481 agcttttgac aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg
     3541 ttggaatctg ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt
     3601 cacagcttta gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca
     3661 aactccagtt gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga
     3721 agaaattcca gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa
     3781 gttggcggct taccacacag cttactttaa cagtggtgct gtactctata ttccagataa
     3841 cgtagaaatc acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc
     3901 gtttaacaag catattatga ttatcgctgg taaaaattct aagattagtt atctggagcg
     3961 tttagagtca cgcggtgaag gaagtgacaa agcaactgcc aatatcacag tggaagtgat
     4021 tgcacgttct ggtgcgcaag tcaagtttgc tgctattgac cgtctaggtg aaaacgtcac
     4081 tgcctacatt agccgtcgtg gtaaattagg caacgatgca agtattgact gggctatcgg
     4141 tgtcatgaac gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag
     4201 ccatgctgac ctcaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac
     4261 tcgtgtaact aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct
     4321 tgaaaaagca actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc
     4381 agatgcccaa caagagagcc gtgttctcat gctttcagac caagcgcgtt cagatgctaa
     4441 cccaattctt ttgattgatg aaaatgacgt cactgcagga catgcagcct ctattggtca
     4501 ggtagatcca gaagatatgt actacctcat gagtcgtgga ttggataagg caactgcaga
     4561 gcgtttggtt gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt
     4621 tcgtgatgaa atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta
     4681 tgttagatgt agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg
     4741 aacctctggt ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag
     4801 ctattaacag ttactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag
     4861 cggaacgagc gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg
     4921 caggctctac aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg
     4981 cacgctttgc tgaggaaatt ctcactgagg gagaccaggt cttgatttca gtaatggaac
     5041 accattctaa tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct
     5101 atgtctatct taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata
     5161 aggttaaatt tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca
     5221 aggaaatcac tcaattagcc caccaagttg gggcaatcat ggtagtggat ggtgctcaat
     5281 ctacacctca tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg
     5341 gtcacaagat ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg
     5401 agcaaatgtc tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatctg
     5461 ctagttggaa ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta
     5521 ttggacttgc gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc
     5581 atgaacagga attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca
     5641 tttacggttc tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc
     5701 tccatcctca cgatcttgcg acagctctgg attatgaagg agtggctgtt cgtgctggtc
     5761 accattgtgc gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt
     5821 tttatatcta taataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa
     5881 aggagttttt caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac
     5941 cattcgaaaa atccacatca ccaagggaag ttagaagacg ctgagcaaat cagtctcaac
     6001 aatccgactt gtggagatgt catcaatctc tctgtcaagt ttgatgcaga ggaccgtttg
     6061 gaagatattg cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg
     6121 acagatgccg ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct
     6181 gaaatggttc aagggcaaaa agatgagcgt caagaccaac ttggagacgc ggcattcttg
     6241 tcaggtgttg ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt
     6301 aagaaaacaa ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta
     6361 ttagaaatga agaaagaaag gatattatgg ctgaagaaag agtagaacca aaaccaattg
     6421 accttggtga atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa
     6481 aaggactcaa cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga
     6541 tgttggagtt ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg
     6601 gagcagactt gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca
     6661 aaccagcccg ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg
     6721 ggattccaga agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag
     6781 tggtttacca caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag
     6841 attccgcact caaggaatac ccagacttat ttaatcaata ctttgcgaag ttggtaccgc
     6901 cgacagataa caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct
     6961 acgtgccaaa aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg
     7021 aaaatatagg tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact
     7081 acgtagaagg atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag
     7141 aaatttttgc tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata
     7201 acgtctataa cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga
     7261 ttgatggaaa cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag
     7321 aaggagcgcg tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca
     7381 cgggtgctaa gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca
     7441 tcgctaaagg tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta
     7501 agaaatctgt ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag
     7561 atactattcc atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag
     7621 tatctaagat ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg
     7681 aggcaactga aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg
     7741 aatacgcagt tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa
     7801 atttgatttt atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg
     7861 tatggttact gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg
     7921 cggctagctt cctagtttgt tctttgattt tgaagatgaa cttgtatcaa aaaatcgcgg
     7981 tttaaaatcg cgatttttta taatttctcg ttaacaaagc ggacaaactg attccaccaa
     8041 acttttaaga agaaggcttt ttcaattttc ttgtctgcta ccatttcgaa actagggcgc
     8101 tctgtggtga tgtaaccttg accaatcaag tccttgtctt cataagtcaa atggccaacc
     8161 actgttccag cttcaagtgg tgctgggatt gctttggaat caggtgtgaa ttgaacagat
     8221 tgggaagatt gattcccaac acgttctatt agatagatat cctctggagc cactgcagtt
     8281 actgtatctt cttttccatc ttgtacaggg gttttgctat cttgataggc atcgccttgt
     8341 tgaacgattt tgcgaagtgt aaatgtagaa gaaatataat ccattaggga agatgtagct
     8401 gtaaatcgag cgtaaggatt attgtcttga tgatctgcat ttaaaacaac tgtgatgact
     8461 ctcatgcctt tttcgacagt agtaccaaca aaagactctc cagccttatc tgttgttcct
     8521 gtttttagcc catcaaaacc accacggtaa gcaggcatac cttctaacat gtagttggtt
     8581 gaagtgattg tcatcccagc aaaagtagaa gaaggttttt tggtgatttc taagacttgt
     8641 gggtattttt tgatgaggtt gcgagcaacg atagcgacat cataagcact aagcttattt
     8701 tcctcatctt ttttagaacc tgggtaaatg ttatccccta gagtttcatt gttaagacct
     8761 gtcgtattga caacagtggc atcctgaatt ccccattcca agagttttgc ccgcatcata
     8821 tcgacgaaat ctttttctga gccagcaatt ttctcagcta gggcaatagc ggcgctgttg
     8881 gcactagata ccagagttgc ttcaagcaac tcttcgacag tataattacg ggcctccata
     8941 ggaatattac tggcttcaga atttgtcgtc aattgataag gataatcaga aatatctaca
     9001 ggagtggaga gggtaatact tccgttttcc aaagcttcat agaccagata aacagtaatc
     9061 aattttgtta tggaagcaat ttcgacaggt tgcgttgcat ccttctcata gagaatttta
     9121 ccagtatttg cctcaacagc aatcgcatgt ttagcggcaa tggtaaaatc ttgagcaaca
     9181 gcagtagaag caccccctaa aagagagaca gttaacaaag ttaaaaatat ttttttcata
     9241 gtagtcttat tctatcataa agaaaaaaaa tattcttgct ttaataattc atctgttaag
     9301 ctttttgaaa atatggtaaa ataaagtgag ggaggtaact catgtttcgt agaaataaat
     9361 tatttttttg gaccacagaa attttactct taaccatcat cttttaccta tggagacaga
     9421 tgggatcttt gattaaccct tttgttagcg tgcttaatac aattatgatt ccatttttat
     9481 tagggggctt tctttattat ttgacaaacc ctattgttac tttcttaaat aaagtctgta
     9541 aactcaatcg tttgcttggt attttaatta ccttgtgtac tttggtctgg ggaatggtca
     9601 taggtgttgt ctatctctta cctattttga ttaatcagtt atctagtttg attatatcta
     9661 gtcaaactat ttatagtcga gtacaagact taatcataga cttatctaat tatcctgcgc
     9721 tccagaattt ggatgtagaa gctacaattc agcagttaaa cttatcctat gttgatattc
     9781 ttcaaaatat cctaaatagc gtatcaaata gtgtggggag cgtcttgtca gctcttatca
     9841 gtactgtttt gattttgatt atgactccag tttttttggt ttatttctta ttagatggac
     9901 ataaattctt gcccatgctt gaaagaacga ttctaaagag ggatcgcttg catattgcag
     9961 gcttattaaa gaatttaaat gcgacgattg ctcgctatat tagtggagtt tcgattgacg
    10021 caatcattat aggttgtttg gcttatattg gctatagtat tattggttta aaatatgctt
    10081 tagtttttgc cattttttct ggtgtagcca atttaattcc ttatgtgggg ccaagtattg
    10141 gtttgattcc tatgatcatc gcaaatatat tcactgatcc ccatagactg ctgattgcag
    10201 tgatttatat gcttgttgtt cagcaggtag atggcaatat cttatatcct cgaattgtag
    10261 gaagtgttat gaaggttcat ccaatcacga ttttagtttt acttttgttg tcaagcaata
    10321 tctatggtgt agttggaatg attgtcgcag tgccaaccta ttctatcttg aaagaaattt
    10381 ctaagttctt atcccgtttg tatgaaaatc ataaaataat gaaagaacga gaaagagaat
    10441 tagctaagta aaagtcagga gaaccctgat ttttctttac tggaagtggc ctttagatta
    10501 gaagactgaa aataagttaa agtcttaaac taattttcac agctaagaat agtagaagtt
    10561 aatctgataa aaatcgaaaa aaccagtgga attccgtgtc agggtaagtt ccactggttt
    10621 tcatagtcta ttaaagttcg aatgaaacct atttataata tattgaaact agaatagt
//
LOCUS       DAVLFB010000074        10494 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLFB010000074 DAVLFB010000000
VERSION     DAVLFB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.074"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..132)
                     /locus_tag="VXD58_001582"
     CDS             complement(<1..132)
                     /locus_tag="VXD58_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 family protein"
                     /protein_id="HEV0676251.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     "
     gene            272..1201
                     /locus_tag="VXD58_001583"
     CDS             272..1201
                     /locus_tag="VXD58_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0676252.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            1401..3851
                     /locus_tag="VXD58_001584"
     CDS             1401..3851
                     /locus_tag="VXD58_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV0676253.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMVIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            3888..5111
                     /locus_tag="VXD58_001585"
     CDS             3888..5111
                     /locus_tag="VXD58_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV0676254.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            5122..5676
                     /locus_tag="VXD58_001586"
     CDS             5122..5676
                     /locus_tag="VXD58_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV0676255.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            5761..6324
                     /locus_tag="VXD58_001587"
     CDS             5761..6324
                     /locus_tag="VXD58_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0676256.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            6339..8285
                     /gene="gyrB"
                     /locus_tag="VXD58_001588"
     CDS             6339..8285
                     /gene="gyrB"
                     /locus_tag="VXD58_001588"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV0676257.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            8367..10094
                     /gene="ezrA"
                     /locus_tag="VXD58_001589"
     CDS             8367..10094
                     /gene="ezrA"
                     /locus_tag="VXD58_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV0676258.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
BASE COUNT         3169 a         1907 c         2240 g         3178 t
ORIGIN      
        1 aataaaaaat aggatttcct tgagattggc atcagattgc gcttcaggtg tataagcttg
       61 gtaatgagga agctgctggt ttaattcttc ttgatagtct acctcatagg attgtaattt
      121 tcttacgggc atgattctct ccttaacagt acatacctat tttatcattt tttcggcaga
      181 gaattattac agaaaggtta caaaaagaat aaagtccctt ttcattttca aagcatggct
      241 gattttggag aaatgtggta taatttttct tatggaaaag attgtcatta cagcaactgc
      301 tgaaagtatt gaacaagttg aacaactact cgaagctggc gtagaccgta tctatgtcgg
      361 tgagaaagat tttggccttc gtctgccaac gacctttagt tatgaccaat tacgtgaaat
      421 cgctaagttg gttcatgatg ctggtaagga attgatcgtt gcggtcaatg ctctcatgca
      481 ccaagatatg atggaccgta tcaagccttt cttagacttc ttggaagaaa tcaagacaga
      541 ctatattacg attggggatg caggcgtctt ttacgtagtt aaccgcgatg gttattcatt
      601 taagaccatc tacgatgctt caaccatggt aactagcagt cgtcagatta acttctgggg
      661 acaaaaggct ggcgcatctg aggctgtttt ggcgcgtgaa attccatcag ctgaactttt
      721 caaaatgcca gagattttgg aaattcctgc tgaagttttg gtttacggtg ctagcgtcat
      781 ccatcattct aaacgtccac tcttgcaaaa ctactataac tttacacata tcgatgatga
      841 aaagacgcat aaacgtgacc tcttcttggc tgagccaagt gatccagaga gccactattc
      901 catttttgaa gataatcatg ggacccatat ctttgccaac aatgaccttg atttgatgat
      961 caaattaaca gaattggtgg agcatggctt tactcgctgg aaactagaag ggctctacac
     1021 tcctggtcag aactttgttg agattgcaaa actctttatc caagcgcgta gcttgattca
     1081 agagggcaac tttagccatg accaagcctt cttgctggat gaagaagttc gtaaacttca
     1141 ccctaaaaac cgtttccttg atacaggatt ttatgactac gatcctgaca tggttaaata
     1201 aaatacatga ttcgttgaga gaaggaagat gaaaacattt cttctctcaa tttttcgtat
     1261 ttcttcacta ttttaaaaaa atcagcaggc tagaatgctc tattcgatgg gatttctaag
     1321 aaaagtagtg ttcttgagtt tgaaaattat cctatgtttg caggtgccaa atggcccttt
     1381 ttttggtata attttttata atgaaaacga ttggtaatcg ctatgttgtg gtggatttag
     1441 aggcaactag cacaggtagt aaggctaaaa ttatccaagt gggaattgtc gtgattgagg
     1501 atggagaaat cgtcgatcac tatacgacgg atgtcaatcc acatgaaccc ttggatgctc
     1561 atatcaaaga actgacagga ttgacagacc aacgtctggc gcaagcacct gatttttcgc
     1621 aagttgccag aaaaatcttt gacttggtgg aggatgggat ttttgtagcc cataatgttc
     1681 agtttgatgc taatctcttg gcggaaaatt tattttttga aggctatgag ctaagaaacc
     1741 ctcgtgttga tacggtcgaa ttggcccagg tctttttccc tgaactggaa aaatatagct
     1801 tgccgatttt gtgtcgagaa ttaggaattc ctcttaaaca cgcacacaca gccctttcag
     1861 atgcccaagc tacagcagaa ttacttcttt ttttacggga aaagatgacc cagcttccta
     1921 aaggtctctt ggaacgcttg ctggaaatgg ctgacgctct cctatatgag tcttacctgg
     1981 ttattgagga aacttatcgc aaccaatcta tcctgagttc tccagacttg gtccaagttc
     2041 aaggtctgta ttttaagaaa acggcagctt ctctggagct acgaaaacta tctcaagact
     2101 tttctaaaaa tatttctctg ttgaaccttg aagtgaggga ggaacaagaa agttttgcta
     2161 aagaggttgg cttgctattg aaagatgaac ctttctctct gattcaagcg ccgacaggga
     2221 ttgggaaaac ctatggctat ctcttacccg ctttatctca atccaaagag cgacaaattg
     2281 ttcttagtgt tccgacaaag attcttcaaa atcaaatcat ggaagaagaa ggtaaacgtc
     2341 tcaaggaagt gttccataca gatattcata gcttaaaggg accacaaaat tatctgaagt
     2401 tggatgcctt ttatcattcc ttgcaggaaa atgatgaaaa tcgcttattt agacgcttta
     2461 aaatgcaagt cttggtctgg cttactgaga cagagacagg agatttggat gaaatcgggc
     2521 aactctaccg ttaccaacat tttctagcag accttcgtca tgatgggaat ttatcatccc
     2581 agagcttatt tgtgacggaa gatttttgga aacgtagtca agaaagggca gagacttgca
     2641 agcttttagt gactaatcat gcctatctcg tgaccagact tgaagataat cctgaatttg
     2701 tcagtgaccg tttactgatt attgatgaag tccaaaagat tttgttagct ctagaaaatc
     2761 tgcttcaaga gacctacgat atacaatcta ttatcgattt aattgataag gctttagtag
     2821 gagaagaaaa cagggttcaa caacggatac tagaaagtat tcgctttgag tgtctctact
     2881 tgatagaaca atttcagtct ggtaaatcta ggaaaaatat cttagattct ctggacaatc
     2941 tccatcagta tttttcagaa ttagaagtgg aaggctttga tgagctggtt cgctatttta
     3001 cagctgaagg tgattactgg cttgaagtaa ctgaaacgag tcaaaagaaa attcagattt
     3061 cttctacaaa atcaggccgt actcttctgt cctctttact tcctgagagt tgccaagtct
     3121 tgggagtatc ggctactctt gagattagtc agagggtttc tttggcagac cttttaggct
     3181 atcctgaagc caaatttgtc aagattgaat ctcggggaaa acaggaacaa gaagtggtta
     3241 tggtcaaaga tttccctctg gtaacagaaa cctccttaga agtctatgcc agagaggtag
     3301 ctgctttact agtggaaatt caagctttcc agcaaccgat tttggttctc tttaccgcta
     3361 aagacatgct tctagcagta tcggatttac ttacagttag ccacttggcc cagtataaaa
     3421 atggggatgt tcatcagcta aagaaacgct ttgaaaaagg tgaacaacaa atcttgcttg
     3481 gtgcagcaag tttctgggag ggagttgatt tttcaagcca tccttctgtg attcaagttg
     3541 taccgaggct tcctttccaa aatcctcaag aacccttgac gaaaaagatt aatcaagaac
     3601 tgaatcaaga agggaaaaat gccttttatg attatcaatt gccaatggtc attattcgtt
     3661 taaaacaggc tttgggaaga agtatgagac gtgaatacca acgttcctta actcttattt
     3721 tggataggag aatcgtcgga aaacgatacg gcaaacaaat agtagcatct ctagcagaag
     3781 aagcgactgt taaaaccatc tctcgatccg aagttgacga ggctattgat agatttttta
     3841 atgaactttg ataaatagta ttgtatgaaa gtataaggtt agtacatatg aaacgttctc
     3901 tcgactctag agtcgattat agtttgctct tgccagtatt ttttctactg gtcatcggtg
     3961 tggtggctat ctatatagcc gttagtcatg attatcccaa taatattctg cccattttag
     4021 ggcagcaggt cgcctggatt gccttggggc ttgtgattgg ttttgtggtc atgctcttta
     4081 atacagaatt tctttggaag gtgaccccct ttctatatat tttaggcttg ggacttatga
     4141 tcttgccgat tgtattttat aatccaagct tagttgcatc aacgggtgcc aaaaactggg
     4201 tatcaataaa tggaattacc ctatttcaac cgtcagaatt tatgaagata tcctatatcc
     4261 tcatgttggc tcgtgtcatt gtccaattta caaagaaaca taaggaatgg agacgcacgg
     4321 ttccgctgga ctttttgtta attttctgga tgattctctt taccattcca gtcctagttc
     4381 ttttagcact tcaaagtgac ttggggacgg ctttggtttt tgtagccatt ttctcaggaa
     4441 tcgttttatt atcaggggtt tcttggaaaa ttattatccc agtatttgtg actgctgtaa
     4501 caggagttgc tggtttctta gctatcttta ttagcaagga cggacgagct tttcttcacc
     4561 agattggaat gccgacctac caaattaatc ggattttggc ttggctcaat ccctttgagt
     4621 ttgcccaaac aacgacttac cagcaggctc aagggcagat tgccattggg agtggtggct
     4681 tatttggtca gggatttaat gcttcgaatc tgcttatccc agttcgagag tcagatatga
     4741 tttttacggt tattgcagaa gattttggct ttattggctc tgtcctggtt attgccctct
     4801 atctcatgtt gatttaccgt atgttgaaga ttactcttaa atcaaataac cagttctaca
     4861 cttatatttc cacaggtttg attatgatgt tgctcttcca catctttgag aatatcggtg
     4921 ctgtgactgg actacttcct ttgacgggga ttcccttgcc tttcatttcg caagggggat
     4981 cagctattat cagtaatttg attggtgttg gtttgctttt atcgatgagt taccagacta
     5041 atttagctga agaaaagagc ggaaaagtcc cattcaaacg gaaaaaggtt gtattaaaac
     5101 aaattaaata aggagaaaat catggtaaaa gtagcagtta tgttagctca gggctttgaa
     5161 gaaattgaag ccttgacagt tgtagatgtc ttgcgtcgag ccaatatcac atgtgatatg
     5221 gttggttttg aagagcaagt aacgggttcg catgcaatcc aagtaagagc agatcatgtc
     5281 tttgatggag atttatcaga ctatgatatg attgttcttc ctggaggtat gcctggttct
     5341 gcacatttac gtgataatca gaccttgatt caagaattgc aaagcttcga gcaagaaggg
     5401 aagaaactag cagccatttg tgcggcacca attgccctca atcaagcaga gatattgaaa
     5461 aataagcgat acacttgtta tgacggcgtt caagagcaaa tccttgatgg tcactacgtc
     5521 aaggaaacag tagtggtaga tggtcagttg acaaccagtc ggggtccttc aacagccctt
     5581 gcctttgcct acgagttggt ggagcaacta ggaggggacg cagagagttt acgaacagga
     5641 atgctctatc gagatgtctt tggtaaaaat cagtaaaacg ggagttattc tctcgttttt
     5701 tatgtggaaa actcagggaa atcatcgctt ttttcataaa aaaatgctat aatgaagggt
     5761 atgaaatatc acgattacat ctgggattta ggtggaactt tactggataa ttatgaaact
     5821 tcaacagctg catttgttga aacattggca ctgtatggta tcacacaaga ccatgacagt
     5881 gtctatcaag ctttaaaggt ttctactcct tttgcgattg agacattcgc tcccaattta
     5941 gagaattttt tagaaaagta caaggaaaat gaagccagag agcttgaaca cccgatttta
     6001 tttgaaggag tttctgacct attggaagac attttaaatc aaggtggccg tcattttttg
     6061 gtctctcatc gaaatgatca ggttttggaa attttagaaa aaacctctat agcagcttat
     6121 tttacagaag tggtgacttc tagctcaggc tttaagagaa agccaaatcc cgaatccatg
     6181 ctttatttaa gagaaaagta tcagattagc tctggtcttg tcattggtga tcggccgatt
     6241 gatatcgaag caggtcaagc tgcaggactt gatacccact tgtttaccag tatcgtgaat
     6301 ttaagacaag tattagacat ataagaaaaa ggaataagat gacagaagaa atcaaaaatc
     6361 tgcaggcaca ggattatgat gccagtcaaa ttcaagtttt agagggctta gaggctgttc
     6421 gtatgcgtcc agggatgtac attggatcaa cctcaaaaga aggtcttcac catctagtct
     6481 gggaaattgt tgataactca attgacgagg ccttggcagg atttgccagc catattcaag
     6541 tttttattga gccagatgat tcgattactg ttgtggatga tgggcgtggt atcccagtcg
     6601 atattcagga aaaaacaggc cgtcctgctg ttgagaccgt ctttacagtc cttcatgctg
     6661 gaggaaagtt cggcggtggt ggatacaagg tttcaggtgg tcttcacggg gtggggtcgt
     6721 cagtagttaa tgccctttcc actcaattag acgttcatgt ccataaaaac ggtaagattc
     6781 attaccaaga ataccgtcgt ggtcatgttg ttgcagacct tgaggtggtt ggggatacag
     6841 atagaactgg aacaacggtt cacttcacac cggacccaga aattttcact gaaacaacaa
     6901 tctttgattt tgataaatta aataaacgga ttcaagagtt ggcctttcta aatcgcggtc
     6961 ttcaaatttc aattacagat aagcgccaag gtttggaaca aaccaagcat tatcattatg
     7021 aaggtgggat tgctagttac gttgaatata tcaacgagaa caaggatgta atctttgata
     7081 caccaatcta tacagacggt gagatggatg atatcacagt tgaggtagcc atgcagtaca
     7141 caacaggtta ccatgaaaat gtcatgagtt tcgccaataa tattcatacc catgaaggtg
     7201 gaacgcatga acaaggtttc cgtacggcac tgactcgtgt tatcaatgat tatgctcgta
     7261 agaataagtt actgaaagac aatgaagata atttaacagg ggaagatgtt cgcgaaggat
     7321 taactgcagt tatctcagtt aaacacccaa atccacagtt tgaaggacaa accaagacca
     7381 aattgggaaa tagcgaagtg gtcaagatta ccaatcgcct cttcagtgaa gccttctccg
     7441 atttcctcat ggaaaatcca cagattgcca aacgtatcgt ggaaaaaggg attttagctg
     7501 ccaaggctcg tgtggctgcc aagcgtgcgc gtgaagtcac acgtaaaaaa tctggtttgg
     7561 aaatttccaa ccttccaggg aaactagcag actgttcttc taataaccct gctgaaacag
     7621 aactcttcat cgtcgaagga gactcagctg gtggatcagc caaatctggt cgtaaccgtg
     7681 agtttcaggc tatccttcca attcgcggta agattttgaa cgttgaaaaa gcaagtatgg
     7741 ataagattct agctaacgaa gaaattcgta gtcttttcac agccatggga acaggatttg
     7801 gcgcagaatt tgatgtttcg aaagcccgtt accaaaaact cgttttgatg accgatgccg
     7861 atgtcgatgg agcccacatt cgtacccttc ttttaacctt gatttatcgt tatatgaaac
     7921 caatcctaga agctggttat gtttatattg cccaaccacc aatctatggt gtcaaggttg
     7981 gaagcgagat taaagaatat atccagccgg gtgcagatca agaaatcaaa ctccaagaag
     8041 ctttagcccg ttatagtgaa ggtcgtacca aaccgactat tcagcgttat aaggggctag
     8101 gtgaaatgga cgatcatcag ctgtgggaaa caaccatgga tcccgaacat cgcttgatgg
     8161 ctagagtttc tgtagatgat gctgcagaag cagataaaat ctttgatatg ttgatggggg
     8221 atcgagtaga gcctcgtcgt gagtttatcg aagaaaatgc tgtctatagt acacttgatg
     8281 tctaaaaaat agggagaagt agttcccttg gtctaaaaaa tatgatataa tcgttacgat
     8341 tgtttcaagt aaaaaaggag tttgatatgt ctaatggaca actaatttat ttaatggttg
     8401 caattgcagt cattttagtt ctggcttatg tagtggcaat ctttctacgt aagcgaaacg
     8461 aggggagatt agaggcgcta gaagaaagaa aagaagaact atacaatctt ccagtaaatg
     8521 atgaagtaga agctgtaaaa aatatgcact tgattggaca aagtcaagtg gctttccgtg
     8581 aatggaatca aaaatgggtc gatttatctc tcaactcttt tgccgatatt gaaaataatc
     8641 tctttgaagc agaaggctat aaccattcat ttcgttttct caaggccagt catcaaattg
     8701 accaaattga gagtcaaatt actttgattg acgaagatat tgcggcaatt cgcaatgctt
     8761 tggcagactt agagaagcaa gaatctaaaa atagtggtcg tgttcttcat gctttggatt
     8821 tatttgagga acttcagcat agagttgctg aaaattcaga acagtatggt caagccttgg
     8881 atgaaattga aaaacaatta gaaaatatcc aatctgaatt ttcacaattt gtaaccttga
     8941 attcatcggg tgaccctgtg gaagccgcag tgattttgga taatacagaa aatcacattt
     9001 tggccttaag tcatattgtg gatcgtgttc cagccttggt tacgacgctt tctacagaat
     9061 tgccagatca attacaggat ttggaatccg gttatcgtaa actaattgat gctaattatc
     9121 attttgttga aacggatatt gaagcgcgtt tccacttgct ttatgaagca ttcaagaaaa
     9181 accaagagaa tattcgtcag ttggaattgg ataatgccga atatgagaat ggacaggcac
     9241 aagaggaaat caatgccttg tatgatattt ttactcgaga aattgctgct cagaaagtag
     9301 tggaaaatct acttgcaact cttccaactt accttcaaca tatgaaagag aataatactt
     9361 tattgggaga agatattgca cgtttgaaca agacctattt acttcctgag acagctgcaa
     9421 gccatgttcg tcgtattcag acagaattag agagttttga ggcagctatt gttgaggtaa
     9481 cttcaaatca agaagaacca acccaagctt attcagttct tgaagaaaat cttgaggatt
     9541 tacaaactca actaaaagat attgaagatg agcaaatttc agttagtgag cgcctgacac
     9601 aaattgagaa agatgatatt aatgcacgtc aaaaggccaa tgtttatgtc aatcgtctcc
     9661 atactatcaa gcgatacatg gaaaaacgca atctgccagg tattccacaa actttcttga
     9721 agttattctt tacggcaagc aataataccg aggatttaat ggttgagtta gaacaaaaaa
     9781 tgattaacat tgaatctgtt acccgagttc ttgaaattgc aacgaatgat atggaagctt
     9841 tagaaacgga aacttataat attgtacaat atgcaacttt gacagagcaa ctcttgcaat
     9901 attctaaccg ctatcgctca tttgatgaac gcattcaaga agcatttaac gaagctttag
     9961 atatttttga aaaagaattt gattatcacg cttcatttga caagatttct caagcattgg
    10021 aagtggcaga gcctggtgta accaatcgct ttgttacctc atatgagaaa acacgtgaaa
    10081 cgattcgttt ttaataaaag aaaaagattt tattgtgtga ggagcagaat caaatctttt
    10141 tctatagttg tggggagatt tacttcattt tctcctgaga ttgagttttt acccagccga
    10201 tttatccacc acctcaaaac agtgttttat actcttcgaa aatctcttca aatcacgtca
    10261 gcgtcgcctt accgtactca agtacagcct gaggctagct tcttagtttg ctttttgatt
    10321 ttcatttagt attaaagtga tttcgtcagt cttatctgca gcttcaaatc tgtactttga
    10381 gtaacttggt aaccgtccaa taacgaagtc tattgaaaaa tctccagact agagaactca
    10441 cggatagttc ctaatctgga gatttcttat ttgcactttt cttgtacaac ttta
//
LOCUS       DAVLFB010000075        10408 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLFB010000075 DAVLFB010000000
VERSION     DAVLFB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.075"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..545
                     /locus_tag="VXD58_001590"
     CDS             <1..545
                     /locus_tag="VXD58_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV0676259.1"
                     /translation="AYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHTMVQRIV
                     KPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGAGIAGVA
                     IGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQLRSEEQ
                     ALHFVPNRNITVVSNFSRTD"
     gene            602..1510
                     /locus_tag="VXD58_001591"
     CDS             602..1510
                     /locus_tag="VXD58_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV0676260.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDKPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1462..2052
                     /locus_tag="VXD58_001592"
     CDS             1462..2052
                     /locus_tag="VXD58_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV0676261.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2055..3161
                     /gene="yqeH"
                     /locus_tag="VXD58_001593"
     CDS             2055..3161
                     /gene="yqeH"
                     /locus_tag="VXD58_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV0676262.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMSHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFITGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            3375..3686
                     /gene="yhbY"
                     /locus_tag="VXD58_001594"
     CDS             3375..3686
                     /gene="yhbY"
                     /locus_tag="VXD58_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV0676263.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3738..4367
                     /locus_tag="VXD58_001595"
     CDS             3738..4367
                     /locus_tag="VXD58_001595"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV0676264.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4367..4960
                     /gene="yqeK"
                     /locus_tag="VXD58_001596"
     CDS             4367..4960
                     /gene="yqeK"
                     /locus_tag="VXD58_001596"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV0676265.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            4961..5461
                     /locus_tag="VXD58_001597"
     CDS             4961..5461
                     /locus_tag="VXD58_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV0676266.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5474..5827
                     /gene="rsfS"
                     /locus_tag="VXD58_001598"
     CDS             5474..5827
                     /gene="rsfS"
                     /locus_tag="VXD58_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV0676267.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            6147..6887
                     /locus_tag="VXD58_001599"
     CDS             6147..6887
                     /locus_tag="VXD58_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV0676268.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6897..7994
                     /locus_tag="VXD58_001600"
     CDS             6897..7994
                     /locus_tag="VXD58_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV0676269.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILAQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNLSIAQQNFGRVPIRIETN"
     gene            8124..9013
                     /locus_tag="VXD58_001601"
                     /pseudo
     CDS             8124..9013
                     /locus_tag="VXD58_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9230..9733
                     /locus_tag="VXD58_001602"
                     /pseudo
     CDS             9230..9733
                     /locus_tag="VXD58_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370715.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            9687..9827
                     /locus_tag="VXD58_001603"
     CDS             9687..9827
                     /locus_tag="VXD58_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676270.1"
                     /translation="MLFYLTKELNSGVKKIGTISTTLVVVAGVSAGVLGGKFDFKFPK
                     KI"
     gene            9843..>10408
                     /locus_tag="VXD58_001604"
     CDS             9843..>10408
                     /locus_tag="VXD58_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676271.1"
                     /translation="MKFKNYLFDLYPYFPKGFSLDNREDPDVCSKALYDDLCKMFFDD
                     DSKEKLKITSVCNKCQNYGNRDYYTLFIDKDKYLLSSDYIGASIYWAQEAGLNDRIIL
                     DHLSISRTIGGHILFPRGGKLETVNQARGGEKGYYDRFDLTLYAIKEWFVENKNTKIG
                     YAIENYHEWFELFSGDDNCKNGFENFVE"
BASE COUNT         3186 a         1983 c         2290 g         2949 t
ORIGIN      
        1 aggcttatat tgaaaagcta gatgtgacaa ccattatcga gaatattcta accaaggtca
       61 tttctctttt actgcttttg attgtatttt atattgctaa aaaaatgctt cataccatgg
      121 tgcagagaat tgtcaaacct tctctaaaaa tgtctcgtca tgatgtcgga cgccaaaaaa
      181 ccatctcacg tttactagaa aatgtgttta attatacgct atatttcttt ttactctact
      241 gcattttgtc gattttaggt ttgccagttt ctagtttgct ggctggagct ggtattgctg
      301 gggtagcgat tggtatggga gcccaaggct ttctgtctga tgtcatcaat ggctttttca
      361 tcctctttga acgtcaactg gatgtgggag atgaggtcgt tctgacaaat ggaccgatta
      421 ctgtatcggg taaggttgtc agtgtgggaa ttcgtacgac acagcttcgt agcgaggagc
      481 aagcccttca ctttgtccct aaccgaaata tcacagttgt tagcaatttc tcacgcacag
      541 actagacctg ttattttaag taatttgtgg tacaatagag ggagtttaat aaggagaaaa
      601 gatggtttta gaaaagcagt tgggcaatgg ttgtacctgg atagacctag acctaggaaa
      661 gttgaataaa ctagaagacc tttctgaaat ttacggtttg gacaaggaaa ccattgaata
      721 cgcactggat agaaacgagc gcgcccacat ggactaccac cgtgaaagtg ggacggttac
      781 ctttatctat aatgtcctag acgtaaaaaa ggacaaggcc tactatgaga cttttcccat
      841 gacctttatt gtcgagcatc gtcgcctgat taccattagt aataccaaga acgcctatgt
      901 cattgaacag atgactcgtt atctggagaa ccatgacacg ctttcgattt ataagtttct
      961 ctttgccagt ctggaaatca tcagcaatgc ctactatcct gtcattgagc agatggacaa
     1021 gagtagggat gaggtcaatg acctcttgcg ccagcgaact accaagaaaa acctctttgc
     1081 cctgtctgat ttggagactg gtatggttta tctgacggca gctgccaaac aaaatcggat
     1141 tttgttagag catattcaag gtcatgcctt gtatcgtagt tttgatgaga ttgagagaga
     1201 acagtttgat gatgccatga ttgaggctca tcagctggta tccatgacag acctaatctc
     1261 tcagatttta cagcagcttt cagcctctta caacaatatt ctaaacaata atctgaatga
     1321 caatttgaca accttgacta tcatttcagt cttgctagct gttttggcag tcgtgacagg
     1381 ctttttcgga atgaatgttc ccttaccttt aacagataag ccccatgctt ggctctatat
     1441 cagtttggct agtgcaggtt tgtggattgt tttatccttg ttactaagga aaattgcgaa
     1501 aaaaagttaa gaaaaggagc cagaatggcg attgaaaatt atataccaga ttttgctgtg
     1561 gaagcagtct atgatctgac agtcccaagc ctgcaggcgc agggaatcaa ggctgttttg
     1621 gtcgatttgg ataataccct cattgcttgg aacaaccctg atggaacgcc agagatgaag
     1681 caatggctac atgaccttcg ggacgcgggt attggcatta tcgtagtgtc aaataacacc
     1741 aaaaaacgcg ttcaacgagc agttgagaaa tttgggattg attacgttta ctgggccttg
     1801 aagcccttca catttggtat tgaccgtgcc atgaaggaat tccactatga caaaaaggaa
     1861 gtggtcatgg ttggtgacca gctcatgaca gatatacgag cagcccaccg tgcagggatt
     1921 cggtcaattt tagtcaaacc cttggtccaa catgactcaa tcaaaacgca gattaaccga
     1981 actcgtgagc gtcgtgttat gagaaaaatc actgaaaagt acggaccgat tacatataaa
     2041 aaaggaattt aactatggaa gaaattctct gtattggttg tggagcaacc attcagacga
     2101 cagataaagc tggtcttggt tttacccccc agtcggcact tgaaaaaggt ttggagactg
     2161 gcgaagtcta ttgccaacgc tgtttccgtc tccgccacta caatgaaatc acagatgtcc
     2221 agttgacgaa cgatgatttc ctcaagctct tgcacgaggt gggagacagt gatgctttag
     2281 tggtcaatgt cattgatatc tttgatttta atggatctgt catcccaggt ttaccacgtt
     2341 tcgtctcggg caatgatgtc ctcttggtag gaaataaaaa agatatcctt cctaagtcag
     2401 ttaagtctgg taagattagc cagtggctca tgaaacgtgc ccatgaagaa ggtcttcgtc
     2461 cagtcgatgt ggtcctaact tcagcacaaa ataaacatgc cattaaggaa gtcattgaca
     2521 agattgaaca ctaccgtaag ggccgcgatg tctatgtggt cggtgtgacc aacgttggaa
     2581 aatcaactct aatcaatgct attatccaag aaatcacggg tgatcagaat gtcatcacta
     2641 cttcacgctt cccagggaca accttggaca aaatagagat tccgcttgac gacggatctt
     2701 atatttacga tacgccggga attatccacc gccaccagat gtctcactat ttgacggcca
     2761 aaaacctcaa gtatgtcagt cctaaaaagg aaatcaagcc taagacctat cagcttaatc
     2821 ctgagcaaac cctattttta ggtggtttgg gacgctttga ctttataaca ggagaaaagc
     2881 aaggatttac tgctttcttt gacaatgaac tcaaactcca tcgtagcaag cttgaaggag
     2941 ctagtgcttt ctacgataag cacctgggaa ctcttctgac accaccaaat agcaaggaaa
     3001 aagaagattt cccaaggcta gtccagcatg tctttaccat taaagataag acagacctag
     3061 tcatctcagg cctaggctgg attcgtgtaa caggcacagc aaaagtcgcc gtctgggcac
     3121 cagaaggcgt cgccgtcgtc acacgaaaag caattattta aaacaaaatc gctgagcgaa
     3181 caaagtgagc tcatatagcg atagttattg ccgtggtgtg attgccaatc tgtagattgg
     3241 caaggggcac aattgagacc aaggctcaat tgtgaggtca ggaaatccat ttttcaaaga
     3301 tgaaatcttt ggaaaattag ttccgaccgt ccctcaccac ctaaaataac tatcaaaaaa
     3361 caaggaagaa aattatgtca ttaacatcaa aacaacgtgc cttcctcaac agccaggcac
     3421 acaccctcaa acctatcatc caaatcggga aaaatggact caacgaccaa atcaaaacca
     3481 gcgtccgtca agctcttgat gcgcgtgaat taatcaaggt tactctctta caaaacacag
     3541 atgaaaacat ccacgaagta gctgaaattt tggaagaaga aatcggtgtg gatacagtcc
     3601 aaaaaatagg acgcatcttg attttgttta aacaatctag caagaaagaa aatcgcaaga
     3661 tttctaagaa agtcaaagaa atctaagacc gaaactccaa ataactgttt tcatagagaa
     3721 ataaagggga ctagcctatg acaatcgaac tattgactcc ctttaccaag gtagagttgg
     3781 agccagaaat caaggagaaa aaacgcaaac aagttgggat tttagggggg aattttaacc
     3841 ctgttcacaa tgcccatctt attgttgcgg atcaagtacg gcaacagttg ggactggatc
     3901 aagttctgct catgcctgaa taccaacctc ctcacgttga taaaaaggaa accatccctg
     3961 aacaccatcg tctcaagatg cttgagttgg caattgaggg aattgacggc ctagtcattg
     4021 aaaccattga gttggagcgc aagggtattt cctacaccta cgacaccatg aagattttga
     4081 cagagaagaa tccagatacg gattattact ttatcatcgg tgccgacatg gttgactatc
     4141 tgcctaagtg gtaccgaatt gatgaactgg ttgacatggt tcagtttgtg ggggttcagc
     4201 gtccacgcta caaggtaggg acttcctatc cagttatctg ggtggacgta ccgctcatgg
     4261 atatctcgtc cagcatggtg cgggacttcc ttgcccaagg tcggaaaccc aactttctcc
     4321 tacctcagcc agtgctagac tacatcgaga aggaggggct ctactgatgt cctatcaaga
     4381 ctatatcaac tgctcccgtg aggccttgtt ggaaaaaatg gcagagcttc tacctgagaa
     4441 acgtctaacc cattgtctag gtgtggagcg tgccgctatg gaattagctc agcgctttgg
     4501 tgtagatgtt gagaaagcaa gtctagcagg tcttcttcat gactatgcta aaaagttgtc
     4561 agatcaggaa tttctagcct tgattgaccg ttaccagcta gaccctgacc tcaaaaactg
     4621 gggcaacaat gtctggcatg gtatggtcgg aatttacaaa attcaggaag atttggattt
     4681 gcatgattct gaaattctgc gagccattga aattcataca gtcggagctg gtcaaatgac
     4741 agacctagat aaggtcatct acgtcgcaga ctatatcgag cacaatcggg catttcctgg
     4801 agtggatgtg gcgcgtgaga ttgcaagtct atcgctcaat aaggcggtgg cctacgaaac
     4861 agctcgtacc gtggagtatc tagctcatca gggattcccc atctatcccc aaacccttga
     4921 aacctataac gcctttgtgc actatttgaa agaggactaa atgaagacgg cttttataat
     4981 cattgatgtt cagaatgttt tagtagaaac tgggttccag acaaaaagtc tattggaaaa
     5041 aatttcttat ttacaaaacc aggctagaag caagaatatt gaaattatct atgttcaaca
     5101 tattgagaac tctgaagctc aaacatcaga agattggcag ttatctgcgc ttttaaatcg
     5161 aaaacctgct gaaaaggtct ttcagaagaa gtataacagt attttcaaag aaactggctt
     5221 aaaagaatac ttggataagc aggggattga aaaattagtt ttatgtggta tgcagacaga
     5281 atattgtgtg gatacctctg tcaaggttgc ctttgaatat ggctatcagc ttattgttcc
     5341 agaaggtgct gtcacaacct ttgatgggga tgacattcca gcagaaacga ttaatgaatt
     5401 ttatgaggac atttgggagg agcgctttgc agatgttcta gattacaaac atattttcta
     5461 aaaagaggat taaatgaacg aaaaagaatt actagaacta gtcgtgaaag cggctgatga
     5521 gaaacgtgcg gaggatatcc tcgcacttga tgtacaagat ttgactagtg tgacggacta
     5581 ctttgtcatc actagctcaa tgaatagccg tcagttggac gctatcgcag ctaatatccg
     5641 tgaaaaagta gctcaagcag gctttaaagg tagccatgtc gaaggcgatg cagctggagg
     5701 ctgggtctta ctggacctcg gtgctgttgt cgtgcatatc ttttcagaag aaatgcgtgc
     5761 ccattataat ctagagaagc tatggcatga ggcgaattca gtagatattt cagaagctct
     5821 tgcttagaag atgaactcag ttgtagtcaa ctgggtttct ttgcttattt tagaaaaatt
     5881 gatagaaagg taaagggcct cgtgtttgtt tcatttcgaa actgaacacg agctaaaagc
     5941 tgagataaaa agtaactttc tttgtatctg acgataggga agaaagtttc ctatgtactc
     6001 tttgctttta aggctctttg tatcttagaa aggatttgaa cccgtcctgt aattctcgga
     6061 aaatagatag ccttccttgg agctcggctc catcgtaagg ctcctaattt tctatcgaat
     6121 tacgggcgga cttggtatca tgttttatgg cgacttatga aacctttgcg gcggtctatg
     6181 atgcggtcat ggacgatagt ttatacgaca aatggacgaa tttttctctg cgtcatttgc
     6241 ctaagaccaa ggagagaaag aaactcttgg aattggcttg tgggacagga attcaatcag
     6301 tgcgcttctc tcaggctggt tttgatgtga ctggacttga cttgagtgcg gatatgttga
     6361 agattgcgga gaagagagca acttcagcca agcaaaagat tgcttttata gaaggtaata
     6421 tgctgaattt gtccaaggca gggaaatacg attttgtcac gtgttattcg gactctatct
     6481 gctatatgca ggatgaggtg gaagtagggg acgtctttaa ggacgtgtac aatgcgctga
     6541 atgaagaggg agttttcatc tttgacgtgc actcgaccta ccagacagat gaagtattcc
     6601 ctggctattc ctaccatgaa aatgcggaag attttgccat gctttgggat acatatgagg
     6661 acgaagctcc tcactccatc gtgcatgagc tgactttctt tatcaaggag gctgacggtt
     6721 cctttagtcg ccacgatgaa gtacatgagg agaggaccta tgagatcttg acctatgata
     6781 tcttgctgga acaggctgga tttaagtcct tcaaactcta tgcagacttt gaggataagg
     6841 agccaacaga aactagcacc cgttggtttt ttgtggcgca gaagtaggag aacatcatga
     6901 ccatcacagg tattatcgca gagttcaatc cttttcataa tggccataaa tacctgctgg
     6961 atcaggcaga gggactgaaa attgttgcca tgtcagggaa tttcatgcag cgtggagagc
     7021 ctgctattgt ggataagtgg acaagggccc agatggcgct ggaaaatggc gcagacttgg
     7081 tagtggaatt gcccttttta gtcagtgttc aggcagctga tttctttggt caaggagctg
     7141 tggatatctt ggatcgcttg ggcattgata gtctagtttt tgggacagag gaagttcgag
     7201 attatcagaa aatcgctgac ttatacacag agaaaggtac tgagatggag aaatttgtgg
     7261 aaaatctgcc tgattccctt tcctatcccc agaaaaccca agctatgtgg aaggaatttg
     7321 ctggtcttga tttttcaggt aataccccca atcatgttct tgctctggcc tatgccaagg
     7381 cggttgcagg acggaatatc aagctgcatc cgattcagcg tcagggggca ggttaccatt
     7441 ctgtgaataa agatgtggac tttgcctcgg cgacagccct ccgtcaacat cagaaggacc
     7501 aagatttctt agaacgtttt atgccttctg ttgccctatt tgagcaggcc agtaaggtga
     7561 tctgggaaga ctattttccc ttgctccgct atcaaatctt gtcaaatccc gacctaacca
     7621 ccatctatca ggtcaatcaa gaaatggctg tgcgcattaa agaagctatt aaaacagccc
     7681 agtctgtgga agaattggtc gagttagtga ctaccaaacg ttacaccaag gcgcgtgtca
     7741 gacgcctctt gtcctatatc ttggcgcagg ctagagaaaa tgtcttgcca gaagccattc
     7801 atgtccttgg atttactgaa aaaggcaggc aacatctcaa gtctttgaaa ggtcaggtca
     7861 gtctagtcag ccgaattggc aaagaacctt gggatgctat gacccaaaaa gcagaccaga
     7921 tttaccaact aggaaatcta agtatagcac aacagaattt tggaagagtg ccgattagaa
     7981 tagaaacaaa ctaagtctac cgaaaggtgg actttttgag attttttacg aataatactc
     8041 ttcgaaaatc aaattcaaat cgcgtcaacg tcgccttgcc gtactcaagt acagcctgcg
     8101 gctagtttcc tagtttgctc tttgattttc actttaaaca tgatttttcg cgtcttcctg
     8161 agattatgtc ttgggatgag tatgctttta caaagggaaa gatgagcttc attgcgcaag
     8221 attttaacaa tctcaatatc atcactgttc ttgagggaag aaaacaagct atcattcgaa
     8281 atcactttct tcgctacgat agagctgtcc gatgtcgcgt caaaattatt actatggata
     8341 tgtttagccc ttactatgac ttggctaaac agcttcgctt tcaaatttct aggctcaggc
     8401 tgaaacagtc tcccagactg tttcactccc gaatgctaaa atcgttcttg atcgctttca
     8461 cattgtgcaa catcttagcc gtgctatgag tcgtgtgcgt gttcaaatca tgaatcaatt
     8521 ggatcgaaag ttccatgaat acaaggctat caagcgctac tggaagctca tacaacagga
     8581 tagccgtaaa ctgagtgata agcgatttta tcgtcctact tttcgtatgc atttaaccaa
     8641 taaagagatt ttagacaaac ttttgagcta ttcacaagac ttgaaacatc actatcagct
     8701 ctatcagctc ttgctttttc acttccagaa taaggaacca gagaaatttt tcggacttat
     8761 cgaggacaat cttaagcagg ttcatcctat ttttcagact gtctttaaaa ccttcctcaa
     8821 agataaagaa aagattatca acgcccttca actacactat tctaatgcca aactggaagc
     8881 gaccaataat ctcatcaaac ttatcaagcg caatgccttt ggttttcgaa actttgaaaa
     8941 cttcaaaaaa cggattttta tcgctttgaa catcaaaaaa gaaaggacga aatttgtcct
     9001 ttctcgagct tagctgactt caacccacta cagttgacaa agcgcctatt ttgtggtata
     9061 atgaagagaa ttgtaaatca taaaaacagg ggaagcaaat gtctaatttt aaagaaaaac
     9121 taccgatatt ccagtcgaac gatgttcaga tttcaaatgt agtcaatgca gtttggtcga
     9181 ttgcgaacac cttacgtgga gcttatcgtt cggataagta tcgagtgatg taattaaaaa
     9241 ttggatgaga agaaaagata caattgagtt tcttggttta tggaaaagct taaataacat
     9301 gaatttcaat tcggtcgaat tcgaccgaat taaatcggaa gctggatata attcatttac
     9361 attgtctcct aagaaatggg tagagaaaac tggagcaatc ggaattatct caaaaggtgg
     9421 aagatatggt ggtgcatttg cacatacaga tattgccttt gaatttgctt tatgtatttc
     9481 tgctgaattt aagatgtatg taattcagga ttacaaaaga cttaagtcag attaaaattc
     9541 taagctatca ttaggctgga atttgaatag agaaatttct aagattaact acaaaattca
     9601 tacagatgca atcaaagaat atctcttaaa agatcttacc aacgaacagt tgtcttacaa
     9661 gtatgtaagt gaagcagata tgatcaatgt tgttttattt aacaaaagag ctaaacagtg
     9721 gcgtgaagaa aataggaaca attagtacaa cacttgtagt agttgcggga gtaagtgcgg
     9781 gtgtgcttgg aggaaaattt gactttaaat ttcctaaaaa gatttaatgg agaataataa
     9841 atatgaagtt taaaaattat ctatttgatt tatatcctta ttttcccaaa ggtttttcat
     9901 tagataatag agaggatcct gatgtatgta gtaaggcgtt gtacgatgat ttatgtaaga
     9961 tgttttttga tgatgattca aaagaaaaac ttaaaataac atctgtatgt aataaatgcc
    10021 aaaactatgg aaatagagat tattatactt tatttattga taaggataag tatttattat
    10081 caagtgatta tataggagcc tctatttatt gggcacaaga ggcaggatta aacgacagaa
    10141 taattttaga tcatttgagt atttcaagaa caataggtgg acacattctt ttccctagag
    10201 gtggaaaatt agaaaccgtt aatcaggcta gaggtggaga aaaaggatat tatgatcgtt
    10261 ttgatttaac actatatgct ataaaagaat ggtttgtgga aaataaaaat acaaaaatag
    10321 gttatgctat agagaattac catgaatggt ttgaactatt ttctggtgat gataattgta
    10381 aaaatggatt tgagaacttt gtcgagtt
//
LOCUS       DAVLFB010000076        10323 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLFB010000076 DAVLFB010000000
VERSION     DAVLFB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.076"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            9..484
                     /locus_tag="VXD58_001605"
                     /pseudo
     CDS             9..484
                     /locus_tag="VXD58_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            522..824
                     /locus_tag="VXD58_001606"
     CDS             522..824
                     /locus_tag="VXD58_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0676272.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEARPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            832..1633
                     /locus_tag="VXD58_001607"
                     /pseudo
     CDS             832..1633
                     /locus_tag="VXD58_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1682..2146
                     /locus_tag="VXD58_001608"
     CDS             1682..2146
                     /locus_tag="VXD58_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV0676273.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2303..2728
                     /gene="lacA"
                     /locus_tag="VXD58_001609"
     CDS             2303..2728
                     /gene="lacA"
                     /locus_tag="VXD58_001609"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV0676274.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2759..3274
                     /gene="lacB"
                     /locus_tag="VXD58_001610"
     CDS             2759..3274
                     /gene="lacB"
                     /locus_tag="VXD58_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV0676275.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3285..4214
                     /locus_tag="VXD58_001611"
     CDS             3285..4214
                     /locus_tag="VXD58_001611"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV0676276.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4216..5196
                     /gene="lacD"
                     /locus_tag="VXD58_001612"
     CDS             4216..5196
                     /gene="lacD"
                     /locus_tag="VXD58_001612"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV0676277.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5430..6266
                     /locus_tag="VXD58_001613"
     CDS             5430..6266
                     /locus_tag="VXD58_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0676278.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6302..6619
                     /locus_tag="VXD58_001614"
     CDS             6302..6619
                     /locus_tag="VXD58_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0676279.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6621..8315
                     /locus_tag="VXD58_001615"
     CDS             6621..8315
                     /locus_tag="VXD58_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV0676280.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8530..9936
                     /gene="lacG"
                     /locus_tag="VXD58_001616"
     CDS             8530..9936
                     /gene="lacG"
                     /locus_tag="VXD58_001616"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV0676281.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            10026..10238
                     /locus_tag="VXD58_001617"
     CDS             10026..10238
                     /locus_tag="VXD58_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV0676282.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         3339 a         1763 c         2114 g         3107 t
ORIGIN      
        1 aaaaccttat ggtattagat tatttctttg acaaaaacct tgtgttttgc ttagaagcgg
       61 ataatcaaga ataactcttt gatcaggtag caagcttgtt ggaggaacga gaaattgtaa
      121 ctccaactta tcgtgaagct ttgatcacgc gtgaaaagtc atttccaact ggtttagata
      181 tggaatttct aggaaaggac ttgcaaatgt aatgattcct cacacagata ttgttcataa
      241 tctagctgag aaagtggtgg ttgttcgatt agagaaaccg gtgactttcc ataatatgat
      301 tgcgccaggt aaggaagtag aagtatcctt gctcttcttt atcatcaata attcaagttc
      361 aagtcaaacg aacattcttg ctcagttgat ggactttttc acaggaaatg gacatcttga
      421 agacctatca aaaatttccg aaccagaaaa actttatgct tatatagatg aagcaatcgc
      481 ttaatcttgt ctattaaaaa ataaaatcgg aggaagtcta tatgattaaa attcttgctg
      541 cctgcggtgc aggtgttaac tcaagtcacc aaattaaaag tgctctagaa gaagaacttt
      601 caaaccgtgg ttacgatgtt cactgtgatg cagttatggt gaaagatgta aacgaagacc
      661 ttatgaaagg ttacgatatc tttacaccaa tcgctgcaac agaccttggt tttgaaccag
      721 gtatcccagt tatcgaagct aggccaatct tattccgtat cccagcaatg agcgttccag
      781 tatttgacaa tattagactt cctgcgaaac aaaatatggt atagtagttc tatgaatgat
      841 gaagcaagta aacaactaac tgatgcacga tttaagcgtc ttgttggtgt tcagcgtacc
      901 acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt
      961 ggacgaaaac ctaaattaag cctagaagac cttcttatgg ccactcttca atagtgcgag
     1021 aatatcgaac ttatgaacaa attgcggctg attttggtat tcacgaaagc aactttatcc
     1081 gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacggtt tcaagaactc
     1141 ctctcagctc tgaggacacg gtaatgattg atgcgacgga agtacaaatc aatcgcccta
     1201 aaaaagaatt agcgaatgat tctggtaaaa agaaatgcca cgctatgaag gctcaagcga
     1261 ttgtcacaag tcaagggaga ttgtttcttt ggatatcact gtgaactatt gtcatgatat
     1321 gaagttgttc aaaatgagtc gcagaaatat cggacaagct ggtaaaatct tggctgacag
     1381 tggttatcaa gggctcatga agatatatcc tcaagtacaa actccacgta aatccagcaa
     1441 actcaagccg ctaacagttg aagataaaac ctataaccat gcgctatcta aggagagaag
     1501 caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atgatttcaa caacctatcg
     1561 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggcatta tcaatcatga
     1621 actaggattc tagttttgca ggaagtctaa taaagaaact agcaaggtca ggccttactt
     1681 tattggtgga catccaggat ttaattgtcc tctacttgat gatgaagtct atgaagatta
     1741 ttatctagag tttgagaaag aagagacttg ctctgttcca cgtcctttcc cagaaacagg
     1801 tatgttggat ttccaagata gaagtccatg gctagaggga caaaaagaaa tagatcttag
     1861 ttatgatctt ttcagcaaag atgcagtgac tttggatgaa ttgcaatctc gaacaattgc
     1921 ccttcgttct cttaaacatg ataggggatt gaaagtgcat tttgcagaat ttccaaacct
     1981 catcatctgg tcaactttga acaagggacc tttcattacc tttgaaccat ggtctggctt
     2041 gtcaacattc cttgaagaag gagatcattt agaagataag aagaatgttt gtctcttaga
     2101 agccaatcag gttgaagaat tagggtttga gatagaagtt ttataaaaaa gtaaaaaata
     2161 aataacaaaa acaaacacaa actgttgact tcttcaaaca atagtagtat attatgtttg
     2221 tgaggactaa ttgactgttt tttaaagaca gccaatacac tgctggaatc cagcatagaa
     2281 aaaataaaaa ggagtataca atatgtctat tgttatcggt gcagatgctg caggtttgag
     2341 attgaaagaa gttgtgaaag acttcttaga aaaagaaaac ttccaccttg tggatgttac
     2401 agctgaaggt caagactttg ttgatgtgac tctcgctgtt gctgcagaag taaacaaaga
     2461 agaacaaaac cttggtatcg tgattgatgc ttatggagct ggtccattta tggttgcaac
     2521 taagatcaaa ggaatggttg ctgcagaagt ttctgatgaa cgttcagctt atatgactcg
     2581 tggccacaac aactcacgta tgatcactat gggtgcacaa cttgttggtg atgaattggc
     2641 taaaaacatc gctaaaggat ttgttaatgg taaatacgac ggtggtcgtc accaaatccg
     2701 ggttgacatg ttgaacaaaa tgggctaatt agattatagt aagaaaggta agttaaaaat
     2761 gagaattgca attggatgtg accacatcgt aacagatgaa aaaatggcgg tttcagaatt
     2821 tttgaaatca aaaggatatg aagtcattga ctttggtaca tatgaccata cacgtactca
     2881 ctacccaatc tttggtaaaa aagtagggga agctgtaact agcggtcaag ctgatcttgg
     2941 agtatgtatc tgtggtactg gtgttggtat caacaacgct gtaaataaag ttccaggtgt
     3001 tcgttctgcc ttggttcgtg atatgacaac agccctttat gctaaagaac aattgaacgc
     3061 taacgttatt ggttttggtg gtaaaattac tggtgaattg cttatgtgtg atatcatcga
     3121 agctttcatc catgctgaat acaaaccaac tgaagaaaac aaaaaattga ttgcgaaaat
     3181 tgaacatgtt gaaagtcaca atgctcaaca aacagacgca aacttcttca ctgaattcct
     3241 tgaaaaatgg gatcgcggag aataccacga ctaagaggta acctatgatt ttaacagtta
     3301 caatgaaccc atccattgat atttcctatc ccttggatga gttgaagatt gatactgtca
     3361 atcgtgtggt ggatgtaacc aaaacggctg gtggtaaggg actcaatgtt acccgagtac
     3421 tttcagaatt tggcgattct gttcttgcta ctggtttagt gggtggcaaa cttggtgagt
     3481 ttttggttga acatatcgat aatcaagtaa agaaagattt cttctcaatt cagggagaaa
     3541 ctcgtaactg tatcgctatt ctccacggag acaaccaaac agaagttctt gaaaaaggtc
     3601 ctgaagtatt ggaacaggaa ggtcaagact ttttggaaca tttcaaaaaa ctcttggagt
     3661 cagttgaagt agtggccatc tcaggtagtc tgccagctgg ccttccagtt gattactatg
     3721 cgagcttggt agaacttgct aatcaagctg gcaagcctgt agtcttggac tgctcaggtg
     3781 cagcacttca ggctgttctt gaatcacccc ataaaccaac agtcatcaaa ccaaataatg
     3841 aagaattgtc tcagcttctt ggaagagaag tttctgagga tttggatgaa ttaaaagaag
     3901 tacttcaaga acctttgttt gcagggattg aatggattat cgtttcactt ggtgccaacg
     3961 gtacttttgc caaacatggt gacactttct acaaggtaga tattcctaga attcaggtgg
     4021 taaatcctgt tggatctgga gactctactg tggcaggaat ttcttcagga cttcttcaca
     4081 aagaatcgga tgcagaatta ctcatcaagg caaatgtcct tggtatgctc aatgctcaag
     4141 aaaaaatgac tggtcatgtc aacatggcca actatcaagt tctatatgat caattaatag
     4201 taaaagaggt ataaaatggc tttaacagaa caaaaacgtg tacgcttaga aaaactttct
     4261 gatgaaaatg gtatcatctc agctcttgca tttgaccaac gtggtgcttt gaaacgcctc
     4321 atggctcaac accaaacaga agaaccaact gtggcccaaa tggaagaact taaagtcttg
     4381 gtagcagatg aattgactaa atacgcttca tctatgcttc ttgaccctga gtatggactt
     4441 ccagcaacta aagctcttga tgaaaaagct ggtcttctcc ttgcttatga aaaaacaggt
     4501 tatgacacaa caagcacaaa acgcttgcca gactgcttgg atgtctggtc tgcaaaacgt
     4561 attaaagaag aaggtgcaga tgcagttaaa ttcttgcttt actatgatgt agatagctca
     4621 gacgaactca atcaagaaaa acaagcctac atcgaacgca tcggttctga gtgtgtggct
     4681 gaagatatcc cattcttcct tgaaattctt gcttacgatg aaaaaattgc ggatgcaggt
     4741 tctgtagaat acgcgaaagt aaaaccacac aaagttatcg gcgctatgaa agtcttttca
     4801 gacccacgct ttaacattga tgtcttgaaa gttgaagttc ctgttaacat taaatatgtt
     4861 gaaggcttcg ctgaaggtga agtagtttat acacgtgaag aagcagcagc cttcttcaaa
     4921 gcgcaagatg aagcaacgaa cttgccatac atctacttga gtgctggtgt atcagctaaa
     4981 ctcttccaag atactcttgt atttgctcat gaatcaggtg caaactttaa cggagttctt
     5041 tgtggccgtg caacatgggc tggatcagtt gaagcataca tcaaagatgg tgaaacagca
     5101 gctcgcgaat ggcttcgcac aactggattt gaaaacattg atgagctcaa caaagttctt
     5161 caaacaacag caacttcatg gaaagaacgt gtgtaagaaa gtcctcctag tttaggaaca
     5221 tgaatctaaa aaaatttaaa aaaagttgta tgtaaaggtt tacaaaataa cttacttgtg
     5281 ctatacttaa atcacaagtt aatacaaggt gagtgttact aagtaatatt aggcatgatc
     5341 acaggtgaat tagaaatcag ctgattttct agttcatttg tggtcatttt ttgtactcat
     5401 atacctttaa gatataaaag gaggttgaca tgtatcgaat tctaaatcca atgaatcaca
     5461 atgtctcgct tgtcagaaat gataagggag aagaggtgat tgtaattggt aaggggattg
     5521 cattcggaaa gaagaagggg gatttgattg ctgaaaatca tgttgagaaa atctttcgga
     5581 tgaagaccga agagtccaga gaaaacttta tggctcttct caaagatgtt ccgcttgatt
     5641 ttatcacagt gacctatgaa atcattgata agctatcaaa gaaatatcat tatccgattc
     5701 aagagtatct ctatgtaacc ttgacagatc atatttactg ttcttatcaa gctctaactc
     5761 aaggaaggta caaggatagt aatctgccag atatttctgc taagtatcct gtcgcttttc
     5821 aaatcgcaaa tgaagctttt gaaatttact gtcagaagct agcagatcat tttcctgagg
     5881 acgaaattat tcggattgct tatcatttca ttaatgctga aggtgaaaat gaagtggaac
     5941 ttgtggagtc gattgataag aggaaagaaa ttctcaggaa tgttgaagaa gttttaacgg
     6001 actatgcaat tcaacgaact aaaaagaata accatttcta tgatcgcttt atgatccatt
     6061 tgaattattt cttggattat ttagacagat ctagagatga taaccaatca cttctggata
     6121 tggaagatca tattaaacaa tcctatccaa aagccttcga gattggttcc aagatctatg
     6181 atgtgattac gcaacatacg ggtcttgatt tgtataaaag tgaacgagtt tatctagttc
     6241 tacatatcca acgtttattg tcataaaaat ttatttaaaa ctatataagg agaattctat
     6301 catgaataga gaagaagtaa cattgttagg ttttgaaatc gtagcctatg ctggcgatgc
     6361 tcgttcaaaa ctattggaag ccttgaaggc tgctgaagct ggtgattttg aaaaagcgga
     6421 cgctctggta gaggaagctg gtagctgtat tgcagaggct caccacgcgc aaacaagtct
     6481 attgactaag gaagcttctg gtgaggactt agcgtatagt gtgaccatga tgcatggtca
     6541 agatcacttg atgacaacta tcttgttaaa agacctaatg caccatttaa ttgaacttta
     6601 caagagagga gttaaataaa atggataagt taataatttt tattgagaaa ggaaagcctt
     6661 tctttgagaa attatcgaga aatatatact taagagcgat aaaagatggt tttatttcca
     6721 gtatgccagc agtattattc tcaagtattt ttatactaat agctgctgtg cccaatattt
     6781 ttgggtttaa atggtctgat gaacagttag cttttatttt gaaaccttac aactactcaa
     6841 tggggatttt agctctctta gtagctggga caactgcaaa atcgttaaca gattcagtta
     6901 atacacgtag tatggagaaa accaaccaaa tcaactacat gtcaactttt ttagcagctg
     6961 ttgttggatt gttaatactg gcagctgatc caattgaagg tggatttgct aatggtcttt
     7021 taggtactag aggattactt actgcatttt tggcagcttt tattacagta aatatctata
     7081 aagtatgtat taaaaataat gtaaccatta gacttcctga agaggttcca ccaaatattg
     7141 cacaagtttt taaagatgtt attccgtttg ccttatctgt tctttcaata tatggattgg
     7201 atttaattgt tagaaatatt tttggtacaa atgtagcaga atctgttggt aaaatattag
     7261 cccctctatt ttcagctact gatggttata tcggtttagc tattgtattt ggtgcttatg
     7321 ctttcttttg gtttgttgga attcatgggc catctgttgt agaaccttta attgtagcta
     7381 tcagttatgc aaatatagaa gctaatgttc agcttgtgca agctggtatg cacgcagata
     7441 aaattttaaa tccagttact caaacttttg ttgtaacaat gggtggtact ggtgcaacac
     7501 tagtagttcc atttatgttt atgtggttat gtaaatcaaa aagaaataga attgtcggac
     7561 gtgcgtctgt agttcctact ttctttggcg taaatgaacc aatcctattt ggtgctccaa
     7621 ttgttttaaa tcctatcttc tttattccgt ttgttactgc tcctattatc aatgtttgga
     7681 ttatgaaatt ttttgtagat gttctacaaa tgaatagttt cagtattatt ttaccttgga
     7741 caacacctgc tccaattggt attgttatgg gaacggcgct ggctccattg tcattcgtct
     7801 tggctataac tttaatcatt atcgacactt taatctatta tccatttgtt aaagtttatg
     7861 accatcaaat tttagaagaa gaacggaaag gaaactcttc atctgaattg aaagaaaaag
     7921 ttgctgcaaa cttcaacact gcaaaagcgg atgctattct tgaaaaagca ggtgtcgatg
     7981 cagcacaaaa taccatcact gaagaaacaa atgtcctcgt tctctgtgct ggtggaggta
     8041 caagtggact ccttgcaaat gctttgaata aggcagcagc agaatacaat gtccctgtga
     8101 aagcagcagc aggcggctat ggtgctcacc gtgaaatgtt accagagttt gatttggtta
     8161 tccttgctcc tcaagttgct tcaaactttg aagatatgaa ggcagaaaca gataagctcg
     8221 gtattaaact agcgaaaaca gaaggcgctc aatacatcaa attaactcgt gatggaaaag
     8281 gtgccctagc attcgtacaa gaacaattcg attaaggcta ggaactgtga aacagtctcc
     8341 tatcgttacg gaaatcgcta tggcgaattt cctaatcgcc ttattaattc gtcggtaaaa
     8401 agatatcgtt tttacctcct catgtcacaa ttcggtgact tggtacaaga agtgagatgg
     8461 agaaggatgg ctcactgact cctctcctct cacttttact ttatttaaat caagaaatag
     8521 gtgaaaaaaa tgacaaaaac acttccaaaa gactttattt ttggtggcgc aacagctgcc
     8581 tatcaagcag aaggtgctac acatactgat ggtaaaggac cagtagcgtg ggataaatat
     8641 cttgaagata actactggta cactgcagaa ccagccagtg atttttacaa tcgatatcca
     8701 gttgatctca agctagcaga agagtatggt gtcaatggca ttcgaatttc tattgcctgg
     8761 tcacgtattt tcccgactgg ttatggtcaa gtaaatgcta aaggtgttga gttttatcat
     8821 aatttatttg cagagtgtca caaacgtcat gttgagccct ttgtaactct tcatcacttt
     8881 gacacgccag aagctctcca ctcaaatgga gacttcttaa accgtgaaaa tatcgaacat
     8941 tttgtagact acgctgcctt ctgttttgaa gaatttccag aagtaaacta ttggacaacc
     9001 ttcaatgaaa ttggaccaat cggtgatggt caatatttgg ttgggaaatt ccctccaggt
     9061 atccagtacg accttgccaa agtctttcaa tcacaccaca atatgatggt gtctcatgca
     9121 tgcgcggtaa aattgtacaa agagaaaggc tataaagggg aaattggtgt tgttcacgcc
     9181 ctgcctacta aatatcctct agatcctgaa aatccagcag atgttcgtgc agcagagttg
     9241 gaagatatca tccacaataa attcatctta gacgcaactt atctaggtcg ctattcagct
     9301 gaaaccatgg aaggtgtcaa ccatatctta tcagtcaatg gtggtagttt ggatcttcgt
     9361 gaagaagatt ttacagcatt agaagctgca aaagacttga atgatttcct aggaatcaac
     9421 tactatatga gtgactggat ggaagccttt gatggagaaa ctgaaattat ccataatggt
     9481 aaaggtgaaa aaggaagctc taagtatcaa atcaaaggta ttggtcgtcg tgtagctcct
     9541 gactatgtat cacgcacgga ttgggattgg attatctacc ctcaaggttt gtatgaccaa
     9601 atcatgcgtg tgaagaaaga ttatcctaac tacaagaaga tttacatcac tgaaaatggt
     9661 ctcggctata aagatgagtt cgttgataac actgtttacg atgatggtcg tattgattac
     9721 gtgaaacaac acttggagat cttgtctgat gcgattgcag atggagctaa tgtaaaaggt
     9781 tacttcattt ggtcattaat ggatgtcttc tcatggtcaa acggttatga gaaacgttat
     9841 ggtctcttct acgtagattt tgaaactcaa gaacgttatc ctaagaaatc agctcactgg
     9901 tacaagaaag tagcggaaac tcagattata gactagtaga attagtcatt agatatagaa
     9961 ttttagtgag tcaaaaagat gttcaaagat tttatccaat ctatttatga aaaaaagttt
    10021 atattataaa tttcgaaaaa tgctctcaaa taccgtgttt gacgagtgaa gaattgaaaa
    10081 gtcttggaaa atggtatgtc tcgactggta aagaatggat ttgtcattca gatgatgagc
    10141 tggaagaatt taaaaatcta tttttaaatt ttatcaatcc tgaagaatgg gatactatct
    10201 cctttgattc agattttatg ccgtttcaac aatcgtaacc aatttctcaa aaaagttaaa
    10261 tcttatattt agtactctgt aaaactctta tctaatcacg ttgcttatac tcaatgaaaa
    10321 tca
//
LOCUS       DAVLFB010000077        10129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLFB010000077 DAVLFB010000000
VERSION     DAVLFB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.077"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..120
                     /locus_tag="VXD58_001618"
                     /pseudo
     CDS             1..120
                     /locus_tag="VXD58_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            218..430
                     /locus_tag="VXD58_001619"
                     /pseudo
     CDS             218..430
                     /locus_tag="VXD58_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            330..776
                     /locus_tag="VXD58_001620"
     CDS             330..776
                     /locus_tag="VXD58_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0676283.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(914..2218)
                     /gene="eno"
                     /locus_tag="VXD58_001621"
     CDS             complement(914..2218)
                     /gene="eno"
                     /locus_tag="VXD58_001621"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV0676284.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2382..2828
                     /locus_tag="VXD58_001622"
     CDS             2382..2828
                     /locus_tag="VXD58_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV0676285.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2825..3628)
                     /locus_tag="VXD58_001623"
     CDS             complement(2825..3628)
                     /locus_tag="VXD58_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV0676286.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3637..3966)
                     /locus_tag="VXD58_001624"
     CDS             complement(3637..3966)
                     /locus_tag="VXD58_001624"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV0676287.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3911..4282)
                     /locus_tag="VXD58_001625"
     CDS             complement(3911..4282)
                     /locus_tag="VXD58_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV0676288.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4341..5774)
                     /gene="glgA"
                     /locus_tag="VXD58_001626"
     CDS             complement(4341..5774)
                     /gene="glgA"
                     /locus_tag="VXD58_001626"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV0676289.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5771..6910)
                     /gene="glgD"
                     /locus_tag="VXD58_001627"
     CDS             complement(5771..6910)
                     /gene="glgD"
                     /locus_tag="VXD58_001627"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV0676290.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6900..8042)
                     /locus_tag="VXD58_001628"
     CDS             complement(6900..8042)
                     /locus_tag="VXD58_001628"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV0676291.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8032..9960)
                     /gene="glgB"
                     /locus_tag="VXD58_001629"
     CDS             complement(8032..9960)
                     /gene="glgB"
                     /locus_tag="VXD58_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV0676292.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3077 a         2232 c         1788 g         3032 t
ORIGIN      
        1 atgaatggtg agttaatcgc tccaatgact tacgaagaga cgatgacgag cgactttttt
       61 gaagcttggt ttcagaaatt tctcttacca acattaaccg caccattggt tattatatta
      121 aaacgaagta agaataggac aaatcgatca ggacagtaaa atctaattct aacaatgttt
      181 tagaagtaga ggtgtactat tctagtttca atctactata ttaatggaca atgcaagatt
      241 ccatagaatg gggaagctag aactcttgtg tgaagagttt gggcataaac ttttacctct
      301 tcctccctac tcgcctgagt acaatcttat tgagaaaaca tgggctcata tcaaaaagca
      361 cctcaaaagg gtattaccaa gttgcaatac cttttatgag gctcttttgt cttgttcttg
      421 tttcaattga ctatataaat tgtctaagcg aaacaaccga taagaattgg cacaaaagcg
      481 accgtatttt tgttaccaat acaggaaaac cagttcatag ttctatcttg agcaagtctc
      541 tccagcgagc aaacgaacgc cttaaaaaac caattcccaa acatctgtcc cctcacatct
      601 tcagacacac cactattagc atcttatcag aaaataaaat tcctttaaaa acaatcacgg
      661 acagggttgg tcattccgac tctgaagtca ctacttccat ctacacccac gtcacaaaga
      721 acatgaaaga tgaagcaatc aatgtactgg ataaagttat gaaaaagatt ttttaaaaag
      781 ttttgtccct tttttgtcct ctaaatacaa aaatagccct tcggataaaa tccgaggggc
      841 tagaaacgtt gttaaatcaa cggccgaact tttgaatttc atggttcggg ataaaatagt
      901 tcactgaact attttatttt ttaaggttgt agaatgattt caatccacga tattcagcta
      961 cttcaccaag ttggtcttcg atacgaagca attggttgta tttagcgatg cggtctgtac
     1021 gtgaaagtga accagtcttg atttgtcctg cgttagttgc aactgcaata tcagcgattg
     1081 ttgaatcttc agtttcacct gaacggtgtg atacaacagc agtgtaacca gcttctttag
     1141 ccatttcgat agcttcaaaa gtttcagtaa gagtaccgat ttggttaact ttgataagga
     1201 ttgagttagc agcaccttct tggataccac gtgcaaggta gtcagtgttt gttacgaaga
     1261 agtcgtcacc aacaagttgt actttcttac caagacgttc agtaagagct ttccaaccat
     1321 cccagtcgtt ttcatccata ccatcttcaa tagtgatgat tgggtatttg ttaaccaatt
     1381 cttcaaggta gtcgatttgt tctgcagatg tacgaacagc agcaccttca ccttcaaatt
     1441 tagtgtagtc gtaaacttta cgttctttat cgtagaattc tgatgaagca cagtcaaatc
     1501 cgataaatac gtctttacct ggtacatatc cagcagcttc aatcgcagca aggatagttt
     1561 caacaccatc ttcagttcct tcgaaacgag gagcgaatcc accttcgtca cctacggcag
     1621 tttccaaacc acgtgattta aggattttct taagagcgtg gaagatttca gcaccgtaac
     1681 gaagggcttc tttaaatgtt ggcgcaccaa ctggcaagat catgaactct tggaaagcga
     1741 ttggagcgtc agagtgagaa ccaccgttga tgatgttcat cattggagtt ggaagaactt
     1801 tagtgttgaa tccaccaagg tagctgtaaa gtgggatttc aaggtagtca gcagcagcac
     1861 gagctacagc gatagacaca ccgaggattg cattcgcacc caatttacct ttgttaggag
     1921 taccgtcaag tgcaatcata gcacggtcaa tagcttgttg atcacgtaca tcgtagccaa
     1981 tgatagcttc agcaatgatg ttgtttacgt tgtcaacagc tttttgtgta ccaagaccac
     2041 cgtaacgaga tttgtcaccg tcgcgaagtt caactgcttc gtgttcacca gtagaagctc
     2101 ctgatggaac cataccacgt ccgaaagcac ctgattcagt gtaaacttct acttcaagtg
     2161 ttgggttacc gcgtgagtct aggacttcgc gagcgtaaac atcagtaata attgacattt
     2221 tttactctcc ttatgagtta aattttttac acctctataa taccttaaaa cccctccttt
     2281 ttcaagaaaa aacgttatct ttgtgcaact tttccttaac tttataaagt aatcgctttc
     2341 ttttgtctgt tttattctaa cttttatgat atactgtttt catgacagat ttatcaaaac
     2401 aattacttga aaaagctcat ggtgggttaa aaataaatcc ggatgagcaa agacgctatc
     2461 ttggtacttt tgaggaaaga gttcttggat atgtagatat tgacacagca aatagccctc
     2521 agttagaaaa aggcttttta tttattttag aaaaccttca ggaaaaagca aagccactat
     2581 ttgtgaagat ttcaccaact atcgaatttg ataagcaagt tttctactta aaagaagcaa
     2641 aagaaactga tagtcaagcc accatagtat ctgaagagca tattacttct ccttttggcc
     2701 tggttattca tagcaatgca ccagttcaag tagaagaaaa agaccttcga cttgcttttc
     2761 caaaactttg ggaagttaaa aaggaagaac cagccaaaac atccttatgg aagaaatggt
     2821 ttagctaaat cttgcacata tttaataagt gcccaatatt ggcagccgtg cgctccagat
     2881 agagactggc atttttcaaa ctatcttcta aaggttcact tttctccaaa atagaaaaga
     2941 cagcttggat attttcaaat ggtaggggag gtaaatcttc agcaagacta ccgcaaatag
     3001 caataacagg aactccaaca ggggttcttt ttgcaacacc tataggcgct ttcccagcaa
     3061 agctttgact atcaagtctt ccttctccaa caacaaccaa gtcagcatct gaaactttct
     3121 tatcaaagtt gattaagtcc aagcaggtat caattccaga cacgatactt gcctgagcaa
     3181 aggcacacaa accagcagca atccccagtc ctccatcatt actggccgtg ccaccaacac
     3241 cgatataaat atctttaatc cctttagaaa tgagatggag aatcaactct ccaataccac
     3301 aagtttggat ttgaagtgga tttcgtttct ctagcggaat ttttccaaga ccaaccaagt
     3361 cagctacttc aaatagtgcc agttcccctt tttgaaaata gcgcatggct tctttttgtc
     3421 caaaagggtc tgtcacttgg atccattttt cttttaggtc aagagaatgt cggatagcat
     3481 ctacagtacc ttctccccca tcaccaacag ggcagaggag acattctaca tctgctatcg
     3541 attgttggaa gcctcttttt attgcttcag ctacctgttg agctgtcaag ctttccttaa
     3601 acgaatccgg tgcaattaca atcttcatat tttccctcat tctaaacagt caatcaaagg
     3661 aagaacttct aaaaaatccc tcttgtcaac atgatgtggt atttcttttt tgagcatttc
     3721 tttggaacaa aaggcgattc ctaactccgc cgacttcaac attaatagat tattaacccc
     3781 atcaccgatt gccaccgttc tttctttaga aagttttagt ttctttctcc atttttccag
     3841 agtttctttt ttgacctggg gacttataat ttgtccaact aattttcctg ttaaaagacc
     3901 ttctttgact tcaagctggt tggcagtgaa ataggcaata ccaagggatt ttgctaatct
     3961 ctccaactat tggtgtaaat ccaccagaca ccagaccaac taggatgcca ttcttttgga
     4021 gaatagagat gaactctggg acatttagcg atagatgaat tgagttgaag acgttatcaa
     4081 agaccaaaat aggaagacct tccaacaagg acactctttt tcttaaactg ctttcaaaga
     4141 ccaactctcc tcgcattgct cgacttgtaa tctgcgaaat ttccgcctca tgacctgcct
     4201 ctctccctaa aagatcaatc acttcttcta ggattaaggt tccatctaca tccaaaacac
     4261 acaagccttt tacttgagac atcagttctc ctctctaaac agcctaaaaa tcgtatgaag
     4321 tcatcatacg attttatcta ttaattaact aaactatggt acaagtcaag gtatgacttg
     4381 caggctgtat cccatgagaa gtcactctcc atagcttgtt tttgtaggtt tctccaaatg
     4441 tctggatggt ttctatacaa gtccaatgct gtttggaaag tccaatttaa ccaataagga
     4501 gatagattgt caaagctaaa gccagtaccg cttccttcga ttggattgaa agcgcgaact
     4561 gtatctcgca agcctccaac ttcatggacc aatggcaagg ttccataacg catagccatc
     4621 atttgagaca agccacacgg ttcaaaacga cttggcatga ggaagaggtc acaagcagcg
     4681 tagatttcct gagcaagttt gacatcaaaa gtgatatttg ctgatagctt gtctgggtaa
     4741 atctgagcaa accatgagaa agctccttca aaggctggat cgccagttcc caaaagaaca
     4801 atctgaacat cttcttgcaa gatatggtga agactttcga ccaccacatc aaaacctttt
     4861 tgacgtgtca aacgagaaac aattcccacc agtggaacgt ctgctctaac aggcaagcca
     4921 actctttctt gcaattttgc cttatttttg gctttcccag acaaatcttc ctgattgaaa
     4981 tgatagtcta aaagagcatc cgtctgagga ttataaagat cagcatcaat cccattcacg
     5041 ataccagata ctttaccaga ctccatttta agaatctgat ccaaattaca tccaaactga
     5101 ctagtcataa tttcatgagc atagctaggt gaaacggttg aaacacggtt cgcatagaga
     5161 atacctgcct tcatccagtt cagacagttg ttccatcgaa gggtgccatc agcgtaacgt
     5221 tcaaggccaa ctccaaacaa atcacccaac attccttctg aaaattgtcc ttggaattct
     5281 aaattatgaa tggttaaaac tgtttcaatg tcctcatagg cttgaatcca acagtatttt
     5341 tccttcaaca agaaaggaat catagctgta tggtagtcat gaacatggag aagatcagga
     5401 ataaagtcaa tcctttccat agcctcaatg gcagccagtt ggaaaaaggc aaagcgttct
     5461 ccgtcatcaa aatcaccgta aacatgacca cggaagaaat aatattgatt gtcaataaag
     5521 tagaaggtta caccatttaa tactgttttc ttaattccac aatactgtct acgccaacca
     5581 acgctcacct caaaatgaag cacatcttca atctgatttc caaatttagc ctctaccata
     5641 tcatagtagg gtaaaatcac tgcaacttcg tgcccagctt ttaccagtga ttttggaaga
     5701 gcgccaatga cgtctcccaa accacctgtt tttgaaaagg gtgcaccctc tgctgctaca
     5761 aataaaattt tcatgaatga atatcctctg ttactttagc acctttctta accacaactg
     5821 gatgttctgc agttcctcga atcacaacac caagctcaac ttcaacccct ttgtccaaga
     5881 tagcatattc gacctgagcc ccttctccaa taacaacacg agggaagagc aggctatctt
     5941 taaccaagct atccttatgg acatgaatat tacgtgatag aacagaatta gctacttgac
     6001 cttcaataat actaccagag gcaaactgag aagtgcttac cttagatgta ttagcatagt
     6061 aagttggctc ttcgtttttg acctttgtat aaatcttttg gtttggtgag aaaagagaat
     6121 agaatttttg tgattcaagc atatcgatat tcgcttgata ataagattta acagagtgaa
     6181 tattggctag atagcccgtg tactcgtagg caaaagctcc ctcttttaca gccaaatccc
     6241 gtaaaacata gcgcaatttc tctggatgtt cttttttagc ttcttcttcc aagtgttcaa
     6301 tcaaccaagg tgtatcaacg acaaagatat ctgtagacat attgaacgtt tcagctgttg
     6361 acttgctatc aaagagttta tgagaaagaa catggtctgt ttcatctaca tccaagattg
     6421 catttacttc tgaaatatct ttcttagcta gttttttata aactacagtg ataggctctt
     6481 ttgttgtact atgtaggtgg aaaacttggt tcaaatcaat gttaataaga acatcgcagt
     6541 tgagggcaac cgtttggttt gagccagaac gtttcaaata agtaagaagc tgttggtagt
     6601 attcttttcc aactgtacta ctttctacac gggtattgta aattcctaga tagtaatggc
     6661 taagaagggt tgataagccc cactcgcgcc ctgaacgaat atggtcaaag actgagctga
     6721 tattatcctg ctggaaaata ccaaagacac tacgaacacc tgcattagca aggcttgaaa
     6781 gtgggaagtc aatcaaacga tatttcccac caaatggcaa acttgctact ggacggtggt
     6841 ccgtcaatgt cgacatatcg tgaaaaccaa ctgtatttcc taaaatggca gaatatttat
     6901 caatcttcat ctgttgctac ccccactact tcattatatc ctacaacttg tacttcatct
     6961 gttccatcaa tttcgacacc gtcagaaata atcgcacctt caccaataat ggcacgttta
     7021 atcttagctc cttgaccaat gatagctcca ctcatgataa ctgaatcaag gacttccgct
     7081 ccttcgcgaa cttgcgcgcc tgttgaaagg atagaatgtt taacagttcc atcaacgaaa
     7141 catccgtcta caactaatga gtcttccaca tgagcatttg ccccgaggaa gtttggtggt
     7201 gaaatcaagt ttcttgagta aatcttccat tgacggttac gactatccaa ggcattttct
     7261 ggagaaatat actccatgtt cgcttcccaa agtgactcaa tagtaccaac atctttccaa
     7321 taaccactaa attcataagc ataaacactt tcacctgact caaggtaatt tggaatgaca
     7381 tttttaccaa agtctgacat gccaaccttg ctcttttcag cagcgactaa catattacga
     7441 aggcgttgcc aatcaaaaat gtagattccc atagaagctt ttgtagattt aggttgagct
     7501 ggtttttctt caaattcaac aatacgattg ttagcatctg tgttcatgat accaaaacgg
     7561 cttgcttctt taagagggac gtctaaaact gctactgtca agctggcatt attatcctta
     7621 tgagactgga gcatatcatc atagtccatt ttgtagatgt gatccccaga caaaatcaag
     7681 acatactcag gattgacact gtcgatatag tcgatatttt ggtaaatagc gtgactagtc
     7741 ccctcaaacc aacgatttcc ttcacttgca gaataaggtt gaagaataga gacacctgaa
     7801 ttaataccgt ctagtcccca gcttgaacca ttcccaatat ggttgttgag agcaagtggt
     7861 tgatactgtg taacgacccc aacattatga atccctgagt tggcacagtt tgatagggca
     7921 aagtcaatga tacggtagcg cccaccaaat tgcacagctg gttttgcgat gctttgagtg
     7981 agtttaccga gacgagttcc ttgcccacca gcaagaatca aagctaacat ttcatttttc
     8041 attttctact cctttttggt ttttatttgt gacggtttta gtagatttca agcgacgttt
     8101 gattttccat acacttgctc ccatagccgg tagggtaaag gttaaggtct gctcataatc
     8161 tttccatagt ccttcttgcg tttgaacagt ttgattatgt tctttccaaa cgcctcccca
     8221 ctcttccaac tcagtattcc atacttcttc gtaaattcct gcaacgggta gtccgattgt
     8281 aaaatctttc cgctcaacag gtaccatgtt aaagatacag actaacattt ctcccttttt
     8341 acccttacga ataaaggaaa gaacactctg gtctcgatta tccgcatcaa tgatttcaat
     8401 accatcatag ctggtatcga tttcccacag acagcgatga tctttgtaaa actggtttag
     8461 ctgagaagtg aaatacttca tcttagcatt cattgggtct tctaggttag accattccaa
     8521 ctgttcttca gatttccatt ctaggaattg accgtattcg ctacccatga agagcaattt
     8581 cttaccagga tgacaaattt ggtacgtata gagattgcgc aagcctgcga attgattgta
     8641 acgatctccc cacatcttat gcatcatact cttcttgcca tgaaccactt catcgtgcga
     8701 gaatggcaag agataattct ccttgaaaac atacataaag ctgaaagtca ccaggttaaa
     8761 gtcatattta cgatagatcg gatcttcttc gtagaaacgg aggatatcat tcatccagcc
     8821 catgttccat ttgtagtcaa atcctagacc accaatctct ttcattcccg taatcttggt
     8881 cgcagacgaa ctttcttctg caatcatcat cacatctgga tattctaact taataacctc
     8941 attcaagcgc tgaaggaaat aataaccttc atagttgaga tttccgccat ctttattagg
     9001 tgtccatgga gcatcatcat agtccaaata gagcatgttg ctaacagcat ccacacgaat
     9061 accatccaaa tgatagacat caatccaatg cttaatgcaa gaaattaaga aggactggac
     9121 ttcatttttt ccaaggtcaa aattaagggc accccaacca tggttatgag ccttattatg
     9181 gtcttggtat tcaaaagtcg gtgtcccatc ataataggct aaggcatcat cgttgatggt
     9241 aaagtgacct ggtacccagt ccacaataac cccaatatta tgggtatgac actcctcgac
     9301 aaaatcttga aactcctctg gtcggccata agcatgctct aaagcgaagt aacccataag
     9361 ctgatacccc caactcaagc ccaaaggatg ggacatcaag ggcataaact caatatgagt
     9421 atagttcatt tcaacgagat aaggaatgag ttcatccttg agctgggcaa aactataagg
     9481 actgccatca gaatttcttt tccatgatcc agcgtgaact tcataaatat tgacaggacg
     9541 ctcttcaaag ccccaacgtt ttcttcgtgc cagccaaagt ccatccttcc atttcttctc
     9601 acgaagctct gttaagattg cccctgttcc tggacgagcc tcatacctga cagcaaaagg
     9661 gtcaatcttc atcagttgat gaccattttg acgtgtgaca tgatatttgt aaatatgccc
     9721 ttcttgagcc atattggtaa agacttccca gaccccaaaa tcatttctta ccattggaat
     9781 ctgattttca atccagttgg taaaatcacc aaccaagtga acagcctgag cattaggtgc
     9841 ccaaacacgg aaggtatagc catgctctcc atttagttct tccctatgtg ctcctagata
     9901 atgttggaga taaaaatttt cacccgtcat aaaggttttt aatgcttctc tattatccat
     9961 atactcccct tctcctgtaa gcgttttcta tgtttttatt atactacctt tttagagaag
    10021 attcaagtaa attactatac ttctttaatt attttgaaaa tctacaacaa gttcacttac
    10081 tcgttcaatt gtaaatcaat attttttcaa aaaattgcga aaactcctt
//
LOCUS       DAVLFB010000078         9980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLFB010000078 DAVLFB010000000
VERSION     DAVLFB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.078"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            21..335
                     /locus_tag="VXD58_001630"
     CDS             21..335
                     /locus_tag="VXD58_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676293.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1019..1180
                     /locus_tag="VXD58_001631"
     CDS             1019..1180
                     /locus_tag="VXD58_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676294.1"
                     /translation="MNNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1272..2120
                     /locus_tag="VXD58_001632"
     CDS             1272..2120
                     /locus_tag="VXD58_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV0676295.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLGAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVW"
     gene            2166..2951
                     /locus_tag="VXD58_001633"
     CDS             2166..2951
                     /locus_tag="VXD58_001633"
                     /inference="COORDINATES: protein motif:HMM:NF024800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HEV0676296.1"
                     /translation="MNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFY
                     YYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTS
                     EIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDEL
                     ESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEIQVYE
                     EKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3219..3785
                     /locus_tag="VXD58_001634"
     CDS             3219..3785
                     /locus_tag="VXD58_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676297.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGVEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4108..4296
                     /locus_tag="VXD58_001635"
     CDS             4108..4296
                     /locus_tag="VXD58_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV0676298.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4358..5173
                     /locus_tag="VXD58_001636"
     CDS             4358..5173
                     /locus_tag="VXD58_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV0676299.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNETHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKMKFLKSNRMLFSKINMVDNQYKKHQNEQGGI"
     gene            5173..6027
                     /locus_tag="VXD58_001637"
     CDS             5173..6027
                     /locus_tag="VXD58_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV0676300.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6139..7023
                     /locus_tag="VXD58_001638"
     CDS             6139..7023
                     /locus_tag="VXD58_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV0676301.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKVL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7186..8925
                     /locus_tag="VXD58_001639"
     CDS             7186..8925
                     /locus_tag="VXD58_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV0676302.1"
                     /translation="MELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIASQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEINSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELD
                     NQLVISPDGFMIF"
     gene            9011..9328
                     /locus_tag="VXD58_001640"
     CDS             9011..9328
                     /locus_tag="VXD58_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676303.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKVHIKLLANQQKHSDAVATIEDEY
                     YVFIAESKIDGKKEVIQCCMGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGS
                     GNL"
     gene            9374..9655
                     /locus_tag="VXD58_001641"
     CDS             9374..9655
                     /locus_tag="VXD58_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676304.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         3570 a         1377 c         2084 g         2949 t
ORIGIN      
        1 agacagtaga ggtactccag attggaatta catggaaaga tatagagata gataaagaga
       61 gagagggaga gatagataaa aagtccaccc ccatttttta tggagaattc aaaaatgtaa
      121 ggttaagcaa agaagaatat aaaaatctaa aagaaaaatt aaactcacac acagatataa
      181 tgataaataa actatccaga tacatggaaa gcagtggtaa gacctatcaa aaccactatg
      241 tgacaatctt aaaatggtat gaagaagata aagacaaact aagacagaaa ggtttaaata
      301 aaaaaatgaa ttatgatgta ggagaatctt tatagataaa aagaggtgga aaggcactat
      361 taaagacttt aagtctaaaa aggtatcaaa gtatgccaga gataaaaaaa tataaatatg
      421 acactttaaa ggcgattaga aaattaaaga aatgctagaa gacttttaga tgacagttac
      481 caagagggtc taattttcac tgctacttat gcagtgggaa ttggaccttt ttatataagc
      541 aaattctatt tcaaaattgg ttaccaagaa agacttcttt tcactgttaa gtataagagg
      601 gtaatttata gaattttgtt acatcaagat aaatattttc tgatggtcaa attttaaaaa
      661 gattaataac tttcgataaa ttttgaacct taacaattga atagaccaag acaagacgtt
      721 aattattttt ggagcgtaat aacctatccg tcaagatctt tcggctgaat aattcggcaa
      781 aaaaagttct gctaaacaaa aagaaagtga gcgataagta agagtttgaa tatagggagg
      841 gataccctat ccgctatgaa gaaaatgagg ataatgatac ttctaaggtt gaagccggct
      901 catccggaat agaggcggag gtgagattcc tatgttgcta atccaaagca cttgtcaatg
      961 tcaaaaaact taattaaaca aataattatt agatataaaa ctttagagga ggtatatatt
     1021 gaacaatgac tggaatggat tggctgattt aatagcaaat ctgattgcaa aatatgctgg
     1081 tgctttagat ttagataatc tacccgatcc aacgccagca aaaaatcaag agatgaaaaa
     1141 tagttttgac atggcaaaaa cacaaattga gacggactaa taaaagtgtt ataatatact
     1201 tgaaatgata gtccaaactt aaattaaaaa aattatatat gatatagaaa aattatattg
     1261 aggttaatac tatgtcaaaa gaaaaaataa aagtatacct ctatacacga gtatctacgt
     1321 caatacagat agaggggtat tcgttagggg cacaaaaatc acgaatgaaa gcttttgcta
     1381 tttacaacga ttatgaaatt gttggagagt atgaagatgc cgggaagtct ggcaagtcta
     1441 ttgaaggtag aatacagttc aatcgaatga tggaagatat aaaatctgga aaagatggag
     1501 tatcttttgt tcttgtgttt aagttatcaa gatttgcaag aaatgccgct gatgttcttt
     1561 ctacacttca gataatgcag gattatggag ttaatcttat ctgcgttgaa gatggcattg
     1621 attcatctaa agatgctggg aaactaatga tttctgtttt atcagctgtg gctgaaatag
     1681 aaagagaaaa catccgtatt caaacaatgg aaggttgcat tcaaaaagca agggaaggaa
     1741 aatggaatgg tggctttgct ccgtatggat ataaacttga agacggcaag ctgtttataa
     1801 atgaggaaga ggcagttgca ataagaacga ttttcgacca gtatgtaaat actacgatag
     1861 gagccaatgg gatatctaaa tacttagaga atcatggaat tagaaaaatc ccaagacaga
     1921 atggtaagaa tcctttgttt gatgcaggtc ttataagaaa gatattaaag aatcctgtat
     1981 ataatgggaa aatagcattt ggaagaagaa ctttagaaaa agttcatggt acaagaaatg
     2041 aatataagca ggttgaacaa gatgaatatc taatatctga agggatacat gaagctatag
     2101 tttccgatga agtttggtaa gctgctcagg ttaagctaaa atctcaagca aagaaatatg
     2161 agcatgtgaa taaaggaaaa gatacacgca cacacttgct ttctggaatt gtaaaatgcc
     2221 cgatatgtgg agtgggaatg tttgggaaca agtgtatcaa gaaaaagaaa gatggcacaa
     2281 agtataaaga tttttattac tatggttgta aacataggca gatgataaga ggtcataagt
     2341 gtacattcag taagcaaatt agagaagaat tgttagatga tgctgttgca gaggtgattg
     2401 tcaagatagt aagtaatccg aaatttgctt ctatgatgca agaaaaaatc aacatgaagg
     2461 tggatacctc tgaaatagaa aaagaaatag ataattacca aaaggaattg agaaagagtc
     2521 attctacaaa gtttaagcta attgaggaaa tagataattt agatgttgaa gataagcatt
     2581 ataagagaag gaaacaggat ttagatgata gactatatcg tatgtatgac aaaatagatg
     2641 aattagaatc atcactaatt gatgcgaaag caaagaaaca gactattgaa gctgaaaagc
     2701 ttacaggaga taacatatat aaggttctga tctattttga taagctctat aaagtcatga
     2761 atgatgtaga gcgtaggcag ttaatttcag ctttgatttc tgaaattcaa gtttatgaag
     2821 aaaaacaatc taacggacaa tggctaaaat ccattacttt taagctacca attatagaag
     2881 aaaatctcaa cataggtttg gacaatgatg aacaagttga atgtgtaagt ttattagaga
     2941 aacgtagtta atcctcaaaa ggttccccga accttttgag tcccactgac gcatcacgct
     3001 gaggcggtat cgctgtttgt acgagttgaa gcatcagcaa agtaaaagta gatgttcagc
     3061 caatgcgacg aggtgagacc gaaggtcgat aaggtgtgtt ctgggtatag gactcgtaaa
     3121 gtaagtagga aatatgatga gatgatacag agagcctgaa ttggtcaggt gacgtgctta
     3181 tttacattag cctgataatg aaaatggaga gattatcgat ggttatattg gaattgtacc
     3241 aaaatgatta ttctaaagat ttagtagcat ttgattccat agaagatggg aaagcctttg
     3301 tagctcaaat cccgggatac actctggaaa cagaagatgg tgttgaagtg gagtatttta
     3361 atcctaaaaa cataccggat tatatggaaa ttatttttaa cggaaatatt gttcctttat
     3421 ctagatttat gttcgatcct ggggaaaatg taaacattat ttggaaagag atttcaaatt
     3481 tatccctcaa aaatgataga gtgatcgaag gatattcaaa aattgatgca tatgtcgtta
     3541 acaaccatga agttaaagcc tatgtcgaaa caagagaaac gaactatcgt aaagcaaaag
     3601 acttcttaga aagccgcgga tatgaaatcg acagaagctt tttcggaagt gaagacggtg
     3661 aagcgattct ttacagaaaa aaagggatcg aagtttggca tttcttgtgt cacttagacc
     3721 caatgtttgt agagattgaa gatgtagaag gatatgttaa agaagaagtt ggagagattc
     3781 aatagtcaac caaagttcct tgtgagtata tacctttgct agtaaaagag tcaaaacgtt
     3841 ataacgaagg ggggggaaga aaacccttga gtgtaagtct ttttcttgtt tagagggaaa
     3901 gtattagagt agccgctcat aatttatact agatttgata actgatttga gacagatttt
     3961 atttctataa atattgtgaa aattttttaa taccaaagat ttaaagttac tgtgttaaaa
     4021 atcctctcca cttttaaact aatgtattat aaaagccagt tgacacaagt gttacacctg
     4081 tgatagaatt aacttgaggt gatgaaaatg gcaaccgata aaaatagaat aatgattagc
     4141 ttggatgata aaaatctaga gaaacttgaa aatttagtag aagatgctag agatagaagg
     4201 ggaatgcgac taacaaagtc ccaggttatt gaattactcc taaataccgt tgattatttc
     4261 gatgatatta tgggggctat ttactcaaaa aaataagagc aaaattgcta ttatttttta
     4321 caaattggtg taacactagt gatataggag tgatgaaatg gttgtttata ttagacaaag
     4381 taaattacca agtgaggttt caattaataa atacaatgct caagtaggtg cctacttaca
     4441 aggagaagaa gttattttat atcaatcttt ttctgaaata aaagagttaa caagtgaaga
     4501 tatagttgta gattatataa tggagactag agcattacta aaaatgatgg gcttaaacgt
     4561 tccggttcat gactatccta ttgagcttaa agagttttat ggtcgaaaga tttatgctgg
     4621 tattttagga gagattgtga atatacctga taattgggga aaatttatta agcctaaagc
     4681 tggttcaaaa gtctttactg gaagagttgt taatgaaacc catgatttaa taggtattgg
     4741 tctacctttc gactatccta tatggattag tgaggttgta gaattcatag ctgaatggcg
     4801 ttgttttgtg ttagatggtc gcgtattaga tgttcgaccc tatacaggtg attatcatgc
     4861 acaatttgat gcaagtgtaa ttgatgaagc gatatcatgt tggaaagatg cgccaatagc
     4921 ttatggacta gatatcggtg ttactcgcga tggcagaaca cttgttgttg aagtaaatga
     4981 tggttatgca ttgggaaatt atggcctatc tcctttaaaa tcaatcaatt ttcatagagc
     5041 taggtggaaa gaaatggtaa aaccctattt tgaaaaaatg aaatttttaa aatccaacag
     5101 gatgttattt tctaaaataa atatggtaga taaccaatat aaaaagcatc aaaatgaaca
     5161 aggaggaatc tgatgcttgg agcaattatt ggagatattg tcggttctgt ttacgaatgg
     5221 aacaatatca aaacaaagga ctttccttta tttcggaagg actgcttttt tacagatgac
     5281 acggttatga cctgtgctgt tgcagaagca attatgaatg gtggacaaaa agacgatttc
     5341 attgacgcga tgaagaaata tggcagaatg tatccgaatg ctgattacgg tgctcggttt
     5401 aatgcatggc taaacagcga taaccgtgag ccttataata gctttgggaa tggatcagct
     5461 atgcgtattt ctccatgtgc ttggatcatg gactgtggtt tttatgcgaa aactggtatg
     5521 tggccatcat ctagaggact tacgagtctt tctgcagagg taactcataa tcatccagaa
     5581 ggtgtcaaag gcgctatggc tacagctgat gctatctttc tatgtcgttt ttactttggt
     5641 ggttattgta gagagtacga gcaatcaatt aacgacaatc ctacagagtg taaaagacga
     5701 attaaggatt atatagaaaa agaatacgat tacaatctat ctcaaacttt agatgaaatc
     5761 cgtcctaact atcgttttaa cgaaacatgt caggaaactg tccctcaagc cattatcgcc
     5821 tttcttgaaa gtagagactt cgaagatgcg ataagaaatg ccatctcact tggtggcgac
     5881 agtgatactc tcgcagcaat cacttgtagc atagcagagg cagcttacgg tattcctgat
     5941 tggatcaagg ataaggccta ttcttacttg gatgaaccct taaaggatgt agttaggcga
     6001 tgggaaaata gaataaaagc gtattaatct ccagtacgtc agatttctct tttctgtatt
     6061 ttcattgtat aataggatta catttacttg catgcgacga aatgtaaaca tttctgttta
     6121 aaattaagga taaaatcaat gaaaaaagca atggtaatta tcaaccctac ttctggtggc
     6181 gagaaggctt tggattacaa agaaaagctg gagaataaag caaaagaata ctttgaatat
     6241 gttgaaacca aaattaccga aaaagtgctg gatgcaacac attttgctga agaagcttct
     6301 cgtgagcagt atgatgcagt ggttgtgttt ggtggagatg gaactgtcaa tgaagtcatt
     6361 tcaggtattg atgagagaga ctacattcct aagttaggga ttatcccagg cggtacgggt
     6421 aacctcatta caaaactttt ggaaatcaat caagacatcg atggcgcaat tgacgaacta
     6481 gattttgatt taaccaataa gattgatatc ggtaaagcaa atgacaacta ttttggttat
     6541 atctttagta tcggttctct gcctgaggcg attcacaatg ttgaaataga ggacaaaaca
     6601 aaattcggta ttttaaccta tgctgtaaat accatgaagt ctgtcatgac agatcaggtc
     6661 tttaacatta aggttgagac agaaaatgga aattatgttg gtgaagctag ccatgttttg
     6721 gtccttttga caaattactt cgctgataag aaaatctttg aagaaaacaa ggacggctat
     6781 gccaacattt tgattctgaa agatgcctct atattctcca aattatccgt cattcctgat
     6841 ttattaaaag gggatgttgt cgcaaatgat aatatcgagt atatcaaagc gcgtaatatt
     6901 aaaatctctt cagatagtga attggagtca gatgttgacg gagataaatc agataaccta
     6961 cctgtagaaa tcaaagtcct agctcagcga gtagaagtat tttcaaaacc gaaagaggat
     7021 tagtatatag agaaagcctt ttttaaggct ttttgtatac tttaaaagat agttccttta
     7081 acaacggaca ttccttgcaa atagtttaca aaaatagtat actggattta ttgagtttga
     7141 aaacgtttgc gtaaaatttg aatgaatact ttaggagaca aattgatgga attgagtgct
     7201 atttaccatg ggcctgagtc ggagtatgcc tatctttata aggataagaa actccatatt
     7261 cgaattcgaa ctaagaaagg ggacattgaa agcatcaact tgcactatgg ggaccctttt
     7321 atctttatgg aggagtttta tcaggataca aaagaaatgg tcaagataac ttctggtacc
     7381 ttatttgacc attggcaggt tgaagtgtca gttgactttg cacgtatcca gtatctcttt
     7441 gagctcagag atacagaagg tcaaaatatt ttgtatggcg ataaagggtg tgtggaaaat
     7501 tctctagaaa atcttcatgc aattgggaat ggatttaagt tgccttatct tcatgagatt
     7561 gatgcctgca aggttcctga ctgggtttca aatacggtat ggtatcagat atttcctgaa
     7621 agatttgcca atggcaatgc tctattaaac ccagaaggga ctttagactg ggattcatct
     7681 gtcacaccta agagcgatga tttctttggt ggtgatttac aggggattat tgatcatatg
     7741 gattacttgc aagacttggg tattactgga ctatatcttt gtcccatctt tgaatctaca
     7801 agcaatcaca agtacaatac gacagattac tttgaaattg accgtcattt tggagacaag
     7861 gagacctttc gggaactggt ggatcaagcg catcatcgtg gcatgaaagt catgctggat
     7921 gcggtattta atcatattgc ttcgcaatct cttcaatgga aaaatgtcgt caaaaatggt
     7981 gaacagtctg cttataagga ttggttccat attcaacaat tcccagtgac aactgaaaag
     8041 ctagttaata agagagactt accctatcat gtttttggtt tcgaggacta tatgcctaag
     8101 ctaaatacag ccaatccaga ggtcaagaat tatcttttaa aggttgcgac ttattggatt
     8161 gaagagttta atatcgatgc ttggcgtttg gatgtggcta atgagattga ccatcagttc
     8221 tggaaggatt ttcgtaaggc agttttagct aaaaatcctg atctttatat cctaggagaa
     8281 gtctggcata catctcagcc ttggctaaat ggagatgagt tccatgccgt catgaattat
     8341 cctttatctg atagtatcaa ggactatttc ttacgaggaa ttaagaagac agaccagttc
     8401 atcgatgaaa tcaatggaga gtctatgtat tacaagcagc agatttcaga ggtcatgttt
     8461 aatctcttgg attcacatga tacagagcga atcctgtgga cggccaatga agatgttcaa
     8521 ctggttaaat cagccttagc ctttctcttt ttacaaaaag gaacaccgtg catttattac
     8581 ggaaccgagc tagccttgac tggaggacca gatccagatt gtcgtcgttg tatgccttgg
     8641 gaacgtgtat caagtgacaa tgatatgctg aactttatga agaggctgat taagattcgg
     8701 aaatacgcgt cagtaatcat ttcgcatggc aagtatagcc ttcaagaaat caactctgat
     8761 ctagtagctc tggaatggaa atacgaagga cggatcctca aagcaatatt caaccaatca
     8821 acagaagatt atcttttaga gaaagaagca gtagcactag caagcaattg ccaagaattg
     8881 gataatcagc ttgtcatctc tccagatgga tttatgattt tctaaaaact agttgatgaa
     8941 gattatggta catttcatac cttatatagt ataataaggc tagttactaa acttgtaaag
     9001 gagaacttaa atgaattgta gaggacatga aacaagacaa agaattgtta gagattttga
     9061 agttcagcct aaagtacata ttaagctgtt agcaaatcaa caaaaacata gtgatgcagt
     9121 agcaactatt gaagatgaat attatgtatt tatcgctgag agtaaaattg atggcaagaa
     9181 ggaagttatt cagtgttgca tgggtgcggc aagggatttt ttagaactaa ttaatcacaa
     9241 agggctacct ctttttaatc cgcttgtagg tgattctcat gtaaataata gacaagaata
     9301 tgacaataca gggagtggaa atttataacc tgaaaagtgg aatgaaactg caaagcagct
     9361 ttataatgct ataatgtggt tgattatttt atggaatgct aagccggata cacctttatt
     9421 taattttaaa gacgaagtaa ttaagtataa aacatatgag ccttttgaaa gcagtataaa
     9481 aagagtaaat actactataa agaatggtag taaagggaaa actctgactg agatgattaa
     9541 tggctacaga gcggataacg atattagaga tgaaatttgt aactttaata ttctgaaaaa
     9601 taaaattcgt gatatgaaag accaacaagg aaatacaatg gaatcttact tttagttatt
     9661 gttgaatttt gggtattcta taaaatatcc taattgagat ttaaatagta gactatacaa
     9721 tatagttaaa atatcagtaa aaacaacact ttattgaggt attggatacg ctttgctaat
     9781 agcctaataa tcacatgtgg agtgttgcta caacgaaaaa ggcgataatc cttgatttca
     9841 aactatttta taagcatttt gtctttgtag ataaaggcaa ttttgacaat aaaaatccta
     9901 aaaggtgaat cgttatagat gtatttgtag atatcgtttg cgcatcgaaa aaattaatac
     9961 aagaataaat atttatggct
//
LOCUS       DAVLFB010000079         9812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLFB010000079 DAVLFB010000000
VERSION     DAVLFB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.079"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(152..907)
                     /locus_tag="VXD58_001642"
     CDS             complement(152..907)
                     /locus_tag="VXD58_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV0676305.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(977..3628)
                     /locus_tag="VXD58_001643"
     CDS             complement(977..3628)
                     /locus_tag="VXD58_001643"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV0676306.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(3640..4602)
                     /locus_tag="VXD58_001644"
     CDS             complement(3640..4602)
                     /locus_tag="VXD58_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV0676307.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLKLNHNTLVDIKSKLESLKLKHSRYYISK
                     ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
     gene            complement(4604..5161)
                     /locus_tag="VXD58_001645"
     CDS             complement(4604..5161)
                     /locus_tag="VXD58_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676308.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            complement(5148..5597)
                     /locus_tag="VXD58_001646"
     CDS             complement(5148..5597)
                     /locus_tag="VXD58_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV0676309.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6010..6405)
                     /locus_tag="VXD58_001647"
     CDS             complement(6010..6405)
                     /locus_tag="VXD58_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV0676310.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(6501..6755)
                     /locus_tag="VXD58_001648"
     CDS             complement(6501..6755)
                     /locus_tag="VXD58_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV0676311.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(6765..8099)
                     /locus_tag="VXD58_001649"
     CDS             complement(6765..8099)
                     /locus_tag="VXD58_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV0676312.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8099..8332)
                     /locus_tag="VXD58_001650"
     CDS             complement(8099..8332)
                     /locus_tag="VXD58_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV0676313.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(8600..8788)
                     /locus_tag="VXD58_001651"
     CDS             complement(8600..8788)
                     /locus_tag="VXD58_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676314.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(8748..8930)
                     /locus_tag="VXD58_001652"
     CDS             complement(8748..8930)
                     /locus_tag="VXD58_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676315.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(8932..9711)
                     /locus_tag="VXD58_001653"
     CDS             complement(8932..9711)
                     /locus_tag="VXD58_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676316.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT         2828 a         2212 c         1786 g         2986 t
ORIGIN      
        1 ataaaagagt tataatcagc actactgata aattcaatac cgatttcgcc atcttaaaac
       61 ttagtcaaac gaataccgtt gatttccagt tcattaagtc gttgacctgt caagacttct
      121 cgttccttca actggaaaag ttaaggggga actaatacaa atcctctacg cttctatatt
      181 caatgatttc atttttgata atattctttt taaactcaaa atgttttaaa ggattatcga
      241 ttaacactaa ctttggaata tcatctagat ttgtgaccaa ggataaaata aaatccgatt
      301 gaaactcctt gactttttca aattcttttg cagttaaacg cacacgcccc ttagattttc
      361 ggataccttt tacttcggca aggtaagaat actcttggac atctacctga aagtcatatc
      421 catcaccata aagacgggca tcggttaact gtccaccttg gaatttttct tctttatcaa
      481 aatgaaggat aaaataattc tcagcctcca ttcccgtttc ctgtagacgt ttgaatttag
      541 ttcgaactat tggtttttca acgatggtta tgcctgtttt cttcccttca ctggcaagta
      601 acattttaac tatttcagca taactatgaa catcttcatt tccaaacatc atatcaatta
      661 aatctttacg aaaacgataa acttcagctt tctgccacca accttttcga ttattgtcaa
      721 aataaggatc aaataaatcc attctatttt ttacaactcc agttgtttca gcaattccca
      781 gagaaaccac atagcgataa aactccgact tgctaaagca ttgaaattct tttataaaaa
      841 gcttgtcaaa cttggcaaga ccgtagccaa ttaaatttaa caattcataa tgagggtgtt
      901 tggacatcac tttctccatc aatagatatt gatttcatta taccaaaaaa cacggctcat
      961 caccgtgttt ctgtgtttat ttcaccaact tcttcatctc atcaatacgt gctacggtca
     1021 catcatactt ggcttggtag tcggcttgtt tgtctttttc tttttggaca acttctggtt
     1081 tagcgttggc gacgaagcgt tcgttagaga gtttcttacc aaccatgtcc agttcttttt
     1141 gccatttagc gagttctttg tcgagacgag ctagttcttc ttcaacattg aggagatcgg
     1201 ccagtggcaa gaagatttcg gctcctgtaa taacgcttga catagccagt tcaggtgcag
     1261 ggatggttga tgcgatttcc aagtgttctg gatttgtaaa gcgtttgata tagttgacat
     1321 tgctgttaaa gaaggcttcc aagtcactat cgcttgtctt aacaaggatg gtgataggct
     1381 tgcttggtgc tacatttact tccgcacgcg cattccgaac agcacgaatt aagtctttga
     1441 gactttccac accagtgtga gccgcaaggt cttcaaaggc tagattaaca gttgggtatg
     1501 cagctgtcac gatagaacct tctgagattt gtccaaagat ttcctctgtc acgaatggca
     1561 tgattgggtg aaggagacga aggatcttgt ccaaagtgta tagtagaaca gaacgtgtga
     1621 tgactttctc ttcttcgtta tcgctataaa ggacttcctt ggtcaactca acataccagt
     1681 tggcaaattc ttcccaaatg aagttgtaaa ggatatgacc agccacacca aactcgaact
     1741 tatcaaagtt ttcagtaact tttgcaatgg tttcgttgag attgtggaga atccagcggt
     1801 ccgtcacatt accagcctca cctgttgcaa cttttgtgac attgtcatgc gccacatcca
     1861 gcgtcaaacc tccattgttc atgaggatat agcgagaaat gttccaaatc ttgttaataa
     1921 agttccatga agcatccatt ttctcataag agaaacgcac gtcttgtcct ggcgcagaac
     1981 cgtttgaaag gaaccatcga agggcatcgg caccgtattt ctcgataaca tccattgggt
     2041 caatcccgtt accgagagac ttagacatct tgcgtccttg ctcgtcacga atgagaccgt
     2101 ggataaggac gttttggaat ggttgacggc ctgtgaattc caatgactgg aagatcatac
     2161 gagacaccca gaagaagatg atgtcgtaac ctgttaccaa ggttgaagtt gggaaataac
     2221 gtttaaagtc ttctgagtcg acttcaggcc agcccatggt tgaaaatggc cagagggcag
     2281 aactgaacca agtatccaag acgtcttcgt cctgagtcca tccgtcacct tctggagctt
     2341 cttcgccgac atacatttca ccatcagcat tgtaccaggc agggatttgg tgaccccacc
     2401 aaagctgacg agagataacc cagtcgtgga cattttccat ccattgaagg aaggtatcgt
     2461 tgaaacgagg tgggtagaat tcgaccttgt cctctgtgtc ttggttggca atggcgttct
     2521 tagccaattg gtccatcttg acgaaccatt gagtagacaa gcgtggctca actacgacac
     2581 ctgtccgttc tgagtgaccg actgaatgga cacgtttttc gattttgaca agggcaccga
     2641 tttcttccaa cttagcaacg actgccttac gagcttcaaa acgatccatg cctgaaaatt
     2701 caaaggcaag ctcattcata gttccgtcgt cgttcatgac gttgacttgt ggcaagttat
     2761 gacgttggcc aaccaagaag tcatttggat cgtgagcagg tgtgattttc acgacaccag
     2821 taccaaactc aggatctgcg tgctcatctc caacgattgg gatgagttta ttagcgattg
     2881 gaaggatgac gtttttacca atcaagtcct tgtagcgcgg gtcttctgga ttaaccgcaa
     2941 ccgcaacgtc cccaaacata gtctcaggac gagttgtagc aacttcaagg gcgcgtgaac
     3001 catcttccag catgtaattc atgtggtaga aggcaccttc tacatccttg tgaatcacct
     3061 caatatcaga aagggctgtg cgagctgctg ggtcccagtt gatgataaac tcaccacgat
     3121 agatccagcc tttcttgtaa aggtccacaa agaccttacg aacagctttt gacaaacctt
     3181 cgtcaagagt gaaacgctca cgagaatagt ctacagaaag ccccatcttg ccccattgtt
     3241 ccttgatggt agtggcatat tcgtctttcc attcccagac cttcgtcaag aaagactcac
     3301 gacctaggtc ataacgcgta ataccctcac cacgtaagcg ctcctcaacc ttagcctgag
     3361 tcgcaatacc agcgtggtcc atacctggaa gccaaagggt atcaaagccc tgcatgcgtt
     3421 tttgacggat gatgatatcc tgcaaagtcg tatcccaagc gtgaccaagg tgaagtttcc
     3481 cagttacgtt tggtggtgga atcacgattg aataaggctt agccttttga tcgcctgaag
     3541 gcttgaaaac atccgcatca agccattttt ggtaacgacc agcctcaacc tcggctggat
     3601 tgtatttaga tgaaagttct ttagacatgt gtgttctcct taattacttt tattgacaat
     3661 tttaaccaaa ccaatttttg aaagttcaat ataaatctct tcaatttcag caaatgctgc
     3721 aagtggtgac atagtataat ttccaggatt atatttaaga tcatacttgc ttatctttga
     3781 aatataatac ctagagtgtt tcaattttaa tgattccaac tttgatttaa tatctactaa
     3841 tgtattatgg tttaacttta aaaacaactt attataatta ccatagagct ctgtaagagc
     3901 attaaagtat tctttcacta gagctatagc gtcaatctta gatgtttctt ggtttagttt
     3961 tatcaactcc cctttcaatt ttttccattt aaagttggaa tttaaaagat aatctaaatc
     4021 aatatagaaa tctattataa cagtctcatc atctgggcta acagctttta cctcatttat
     4081 tggtaagtca gtatgttgcg aataatttct caaagaatgg caaaatcgat aagcaaaatt
     4141 ggtatcatac tgatagcttg caaaaccatg tatacaatca aactcctcac tatctttgga
     4201 atatttttgt ttaatttggt tttcgttaaa atcgacaaac aattttccac tggataataa
     4261 atgaatcatg aaacgattta catctgcctc ttcaatttct aattcctttg actcaagttt
     4321 atttttaata cttaaaaaat ccgagacatc tgaaataaac aattcgaaca tattcttgat
     4381 tttttgaaaa ttcttttgaa tctcaaaaat ttggtccgag actgacatta tttccttggt
     4441 tggttcaata tcagagatga caatacgttt ttcattgttg ttaatttcta aataagctaa
     4501 tcgctcgata atatcactat catttaattc agcatactca acatcaattt ggcgtaactg
     4561 cttctcagat agagagctta cttcctcttt tacatcgctc atcttattcc tcccactcgc
     4621 tcttcagcaa gccatatatc agactgtcac agcagttgcc ttgagcatct ttgcggtctc
     4681 ttatgcgagc ttcgagggta aagccaagct tttccgcgac tcgtttactt tgaaggttat
     4741 atccaaagca agttagttca atcttgtgaa gacccaaatc tttaaaggct aagtcaatca
     4801 aggcacgcgc agcttctggc acataacctc gcccccaata gtctgggtgt aaggtatagc
     4861 cgatttccag cacatcatct tcatggcgat ggttgaaatc gacagagcca acgatcttat
     4921 ccgttccttt gacgacaatc ccgtagcctg ctgggagatt ttccttttga ttacgctccg
     4981 gaagaatgtg ctccagataa taaatctcat cttccaaggt cttgactgga ggaaaacctg
     5041 ctggataggc gacctctggc aaactagcat agtcaaagat atcctcagca tccgccacag
     5101 tacggactcg taaaacgaga cgttctgttt cgatttgatc tggcagctca gttcttgcca
     5161 tccttcttcc tcgctttttt gatgaaactg cccttcatat ctacacgctt atccagatag
     5221 cgataaacgc gctgatatcc atctcccatg aaataggttg gggcaaacag ttgattttta
     5281 aaatgtccct tttcatccag gagttctggg gcaacaagtc gctcaagaat cttggcaaag
     5341 atgtggcaaa taccgtcttc ctcaacaatc ctatctaccc tacaatctaa aacaagtgga
     5401 caggcgtcta aaataggagt ctgagttcgt tcagaaattt cataatgcac tcccaaacgt
     5461 tccaatttct cctgatgact gataaaacca gcctgctcca tcgcaagcat agaagtttca
     5521 tcagaaatat tcacagtaaa tttttgatac tgtttgatct gctctgcggc attctctctc
     5581 gcaacgactc caatcacaac ccaatctcct agactataag aggaactaca ggtcgtgatg
     5641 ttatagccaa aattctaatc ttgatatcct aaaataaaaa caggaaaacc ataatatagt
     5701 ttacttgtgt taaaagattg cttcataaca accccctttg actaagacgt aaaagaaaag
     5761 ccctgccatc tacatgacag ggacgaatgt gtttatccgc ggtaccaccc aatttcgggc
     5821 agttgcccgc aactctcgtc tttaaagcaa aaagacactt ttatttcatt ttttcagtca
     5881 ttagcaacca gttttgacac atttgtggac ttctcagcgc cgccactttc tgtaaaatgt
     5941 gggattcaaa acacctctaa tggctctatt gtagcaagtt tttctcggaa atgcaaggct
     6001 caagaaaaat tacttgaact tgatgccatt ttctttcaat ttcttgatgg tagctgggcc
     6061 gattcctttc aaggcaagga gttctttttc agtccagttt ttgaaatctg aagcagactt
     6121 gattccttca tcatagaaag tcttggcacg atcaagtgga agtccaccca agtttgctgc
     6181 aaaatcttct acagaattag ctactttttg agcttgctct ttggtagctt ctactgcttg
     6241 ttcaactttt ttagaaacag ccttaccagc tgcgcttgct gactgttctg cacgcttcac
     6301 gacttttttt gtctcatcta caaccttagt cacagtactt gaaaaggcac ctgcgcgacg
     6361 gaggccattt cgtaattgtt ttttacgatt gagtttcttt gacatgataa aatcctttca
     6421 ataaaaaatc cctgctctga caaggattta acataaacat tctatcaaga ttttagtgaa
     6481 aaatcaagaa tctttctcgt ttaataattt cgctcaccaa acaagccttc tggcaaatca
     6541 tggaatccct tgcctgcttt gaagttttca aacttaccaa gcaagaactg ataagcatcc
     6601 aagcgactct cgtagcgaat catggcatct ttggcacgct cgtcctggtc ttcttcgtaa
     6661 actttttgac tttccttgtg cgccatgtcg ttaatcatga tttgggtatt gacccaagct
     6721 tcaaattcct tcataaattc ttgttcgtaa ctcatttttt ctccttattc taatccacga
     6781 aaatagtccg tatcaatctc acggtagggc tggatatcct gaacatactc caacactcct
     6841 tggaattctc cgtcttcatc gtgtactgca gcataggtga tgtggacaaa cttacctcgc
     6901 gactcagact tgaaccacat ttcatacttg tccttggtcc cctcacgaag ccccttcatg
     6961 atagttttga ccttgtccaa gtacttaggc ggatggcaga gttcgacatt gcgcccgact
     7021 tgagacggcg tccgtttgaa aatcatctca tcagctggcg tattgtcatt gtaatactgg
     7081 aaaatatcat ctttattgac aaaggtaatc tccataggga ggtgattgag gatgagattg
     7141 gcctgctcga ctgaaagata gccattacca aaagcctgtt gactatggcg gtccagcaca
     7201 gcttcctttt ccttaggggt aaaggtaatg gtaaaatggc cttctggcgt atctatgact
     7261 tgttgaactt gaccttctgc cgtatctagc tgtacaggct cctctgcaat cttttcctca
     7321 ataaagctct gtcgttctgg cacccatttc tctgacggac ggatgatggc atagccatag
     7381 gcatcgctct cctccgcaat ctgaagccag tcatcctgag taaaagactc aaggagaatc
     7441 atgaggagga tggactcttc cttgaaaatc atactttcaa actctgtcgc aaaagcttca
     7501 aaagcttcct ttacactgct aattgacact tctggtagtg acttggctat cgttagagct
     7561 gtttgaaaga gttccctaat ctgatcatcc actccccaca taactttggg aggtgaatcg
     7621 tgtccatagc gctccatgat aggaaagaag agttcttcct tacgttggta atggatgtca
     7681 aattgaccca caagtcccat ctgacgcacc aaacccttac gcatctccgc cagcatttcc
     7741 tcgtcttcca tagactcata ggtatctaac aatctacgaa tgcgaatcaa ggccgcacgg
     7801 agagccagat tttcttcttt gaagacacga actgggtgac ctggatgctc agtatctgaa
     7861 acttcgacac ctttgatagc atttttaaaa agattggcat ggacatcaca gagttccata
     7921 acatcttcaa aggtgacacc tgagtctgag ttcatcagct cgtgctccat aagggaaatc
     7981 tcaatggctg agacacccgc aaaggtcgca tcaaaacgct cctgaaccga ctcaggagag
     8041 gcgccattat gcaattctaa taaaatatcc cgtaggacat gaatccgttc atctgccatt
     8101 agtctaatcc aatcacttcg tagccattcg cttccagtgt gcgtacaatc ttgtccatag
     8161 gagttcctgc tagcttagaa ccttgtttaa gtgatacttt acgaccaact gtattgcgca
     8221 tcaagggatt ggcaaggggt ttaaaaccca gctccactag aatttccaag acttctggat
     8281 gcttgtccac cacttctgca acaggaattg acacatcgat gatattgtcc atgacgacct
     8341 ccattatatg ttctaatact caatgaaaat caaagagcaa actagaaagc tagccgcagg
     8401 ttgctcaaaa caccgttttg aggttgtgga tagaactgac gaagtcagta acatatatac
     8461 tgcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat gattttactg
     8521 cttatattat accatatgga gagagattaa aaaactagca gtggagttcc tgctagctct
     8581 ctgcgacttg gattaccatc tagataagtt taaagagcca atccaatatc ttaaataata
     8641 tcttgtaaaa cagcaattga actgcaaagg aaatgatcat ccaaaagaat ttcacaatcc
     8701 caataatcgg tttgataaaa agaatagcaa gaagaccaaa aaaaatttca ttctctctcc
     8761 tctggcctaa tgagccaccg agccgtatcc attagcttat ctgcaataaa aagttttccc
     8821 agaccaacaa cgacatggtc agctttcttt atcgttttca tcacttttac accttgcacg
     8881 gtcaaaaagt aattaccata aattttatca aaagctttga taggattcat attactctcc
     8941 tttaatgatt tcagcctctt ttcggattgt ttcaacatct tcttgatatt gaacttcact
     9001 atagttgact agcttggata attctttctg caagctttcc cccattggtt tcatagttgc
     9061 aaaggttaaa ccacctgcaa tgataccacc caagatagga ataaatttcc ctattccttt
     9121 ggccagccct cccttggtaa gattcacacc aaatattttt aagacttttt tcaaaatagg
     9181 gtaccaaagc gtctttgtta aagctttatt aggcactgtt ttcattacct gtttggcaat
     9241 tgttatacca ccagcacata gcaaagcagc ggttccattc acccctaaca ttacgcctag
     9301 ataaagcaag agggtatttt gagcatcttc actcaactcc tcgcgtgaag cccaaagatc
     9361 ctcataacca taaatataac ctaattcttg agccaatttc agagagaaag cataaaattg
     9421 agccacatca gctggaatgg taattgccat agcaagccct ccagctaatc ctgccaaaac
     9481 agaagtccca ctcgctaata atacattgtc ccgaatacaa gcattagcta cacgatctaa
     9541 tatttcttga gatagaagag tcgttgtacc ttgttccaat aaggtaggaa tatcttttgg
     9601 atccaattct ttggaaaact tatccactaa aaatttcgaa cgatcaacct taacaacagg
     9661 tagtttcacc acttgttgca atacttgcat agctaagtct tgttcagcca tatacaaact
     9721 ccttatttct tttttacacc ttatcatatt atatttccta caaaaaaagc attttctaat
     9781 tagataacat taaaaaatac agaacccctt tt
//
LOCUS       DAVLFB010000080         9774 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLFB010000080 DAVLFB010000000
VERSION     DAVLFB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.080"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(268..1032)
                     /locus_tag="VXD58_001654"
     CDS             complement(268..1032)
                     /locus_tag="VXD58_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676317.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(1025..1795)
                     /locus_tag="VXD58_001655"
     CDS             complement(1025..1795)
                     /locus_tag="VXD58_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676318.1"
                     /translation="MLYLLGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDFLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
     gene            2042..2719
                     /locus_tag="VXD58_001656"
     CDS             2042..2719
                     /locus_tag="VXD58_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676319.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQKKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEQGKEF
                     LEKEWGI"
     gene            complement(2779..4575)
                     /gene="pepF"
                     /locus_tag="VXD58_001657"
     CDS             complement(2779..4575)
                     /gene="pepF"
                     /locus_tag="VXD58_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0676320.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(4586..5329)
                     /locus_tag="VXD58_001658"
     CDS             complement(4586..5329)
                     /locus_tag="VXD58_001658"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV0676321.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMEKRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(5331..6281)
                     /locus_tag="VXD58_001659"
     CDS             complement(5331..6281)
                     /locus_tag="VXD58_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV0676322.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(6419..6847)
                     /locus_tag="VXD58_001660"
     CDS             complement(6419..6847)
                     /locus_tag="VXD58_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0676323.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(6828..7916)
                     /locus_tag="VXD58_001661"
     CDS             complement(6828..7916)
                     /locus_tag="VXD58_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV0676324.1"
                     /translation="MKKIGTYLVYVLAFVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(7935..8405)
                     /locus_tag="VXD58_001662"
     CDS             complement(7935..8405)
                     /locus_tag="VXD58_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV0676325.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVLSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(8696..9148)
                     /locus_tag="VXD58_001663"
     CDS             complement(8696..9148)
                     /locus_tag="VXD58_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV0676326.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(9185..9364)
                     /locus_tag="VXD58_001664"
     CDS             complement(9185..9364)
                     /locus_tag="VXD58_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV0676327.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            complement(9501..9680)
                     /locus_tag="VXD58_001665"
     CDS             complement(9501..9680)
                     /locus_tag="VXD58_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676328.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
BASE COUNT         2963 a         2301 c         1719 g         2791 t
ORIGIN      
        1 aatgaacttg taatgataga gccttatcac aaaaatcaca ttcctgtctc aaaaattgcg
       61 aaaaactagt tataataaag tgaaataaaa actatacaaa ttgattaggg aatttaaaac
      121 aatttctaac aatattttaa aaactgaacc gtactatttc aacttccata aattacaata
      181 ttgataggca cagttcccat acctgtccaa attcgaataa cgtttagtat aaattagaat
      241 atgactacaa catcacaaat aattagacta tctataatat ctacgatgta acatatacat
      301 aggaagcacc gttatcaaga gcgccaaggc tatcaaccag ataagatgaa gatacaaacc
      361 tgtcacctca cctccccata taatacgaat catgcgggta gcgtgataca agggagaaaa
      421 ccaagtcaaa tcaccaagta ttgggggcaa ggaagtaatg ggaaagaagg tatctgaaaa
      481 catataaaga ggacggatta ccaaaacatt gtagtaattc agaaattccc tagacggcgc
      541 caagtaggta aagattagtc ccaaaacacc aaacaaataa cccactataa ataaaatcag
      601 aggaatcaat aacatcaaga catgaaaatg cgctgtgata agcatggaaa tcagccaaaa
      661 aatcccaccg aaaatcactg cacgaatacc tgcccagata gcctgaccca ccacaatatc
      721 attcgtattg attggcgtca tcaacatagc atcataagtc ttttcaatct tatactggat
      781 aaaggcattg accgttgttt ctgcagtggc cgcagacatg gcagtcgcag caattaaacc
      841 tgtaatgaca aaatcacggt agagcatacc gttaacatgc gtcaaataag caccaactcc
      901 aaatcccatt gctaaaagat aaaagagggg gtcgataagg ttggggacaa ttgaaatttt
      961 aaataaattt ctaaaactca tgtagttgcg taccacaacg tgtccgatgg agtgaaatga
     1021 taacttaaac atgcaagtcc tccttctctg tcccagtcaa ccccaaaaaa agcgtctcaa
     1081 ggttgtcagc atcatacttg atgagcaagt cttctacagt tccttcttct ttgatagtcc
     1141 cttggtggag catcagcacc ctatctgtca aatattcaat ctcattcata tagtgggaag
     1201 taataatgat ggaaacacct aattccttca actccaaaag tttatcccaa ataatattgc
     1261 gggattgaat gtctaaacca actgtcggtt catctaaaat gataatatca ggccgattga
     1321 tgagagctct tgccaagaca agacgtcgtc gcatcccacc cgacagctga ttcaccatct
     1381 tttgcgcatg tttgctcagt tgaacaaaat ccaagagaaa atcgattctc ttcctaagtt
     1441 cgacagatga gataccataa catagaccgt gagcaaaaag attatcataa acattcaagg
     1501 tcatatccag catatcctcc tgccctacaa tacccagaca agatttactc ttacgacgat
     1561 tggtttcaat attttgcccc aagatatcaa tctctcctga ggaaattgag gtcagtgagt
     1621 aaatcatctt taaagtggat gttttgccag caccgttgat tcccaagata cccaaacact
     1681 ctccttctcc caattcaaag ctaattccct tgacagcttc ataagaacca tattgctttt
     1741 ttagattttt aacacttaac ttcttcactc tttctttccc caacaaatag agcatgttgg
     1801 atactttgtt aattcatctc acttttgaaa aaatgttgag aatgtaatct taagattaac
     1861 gtccttcaac tgtggatact gctgcaatta cttttgtaat tttacctcca tttaaactca
     1921 gacaataaaa tttaaaatat tctctagtaa aacattttta aaatctattt ttcttgtgct
     1981 ttgattatag tataataaaa gatagaaaat aatcgatata tgaaatttgg agaaaatttt
     2041 tatggaacca gatattttca tcatcacaaa actcttatca gacccttctc ggatgcacat
     2101 tttagatatt ctaatggatg gaaaagctca cacggttaac gaaattgcca gctttaccaa
     2161 aatcaagcaa catactgtca gctatcacct caaattactt accgaagcgc aggtaaccac
     2221 tctacaaacc tatggacgtt ttcattatta ttctttaaaa agtgctgcca ttgcagaaat
     2281 gctggaattt ctcagcttct actctcctca gcgtgatgtc aaaagttata aacaacacat
     2341 tcacaaaaag gaattaaagg tcgctcgcac ctgctatgac catattgctg gtgagcttgg
     2401 catttccatt accaattatc tcttgcaaga aaatctcctc agcgaatcag aaaaagactt
     2461 ccaactcact gaagctggga agtcttattt ccaaaaaaaa ttacacattg atacggatga
     2521 attgaaaaag aaaaaacgga aattttgccc taaatgtttg gattggagcg aacgtaaaaa
     2581 tcatgtcggc ggagctctcg gcaatgctct tttagaattt ttcagtgaaa aacaattggt
     2641 catccctgca caaacgccac gttcacttac cattacagag cagggcaaag aattcctcga
     2701 aaaggaatgg gggatttaaa catggacttt taagcaaata actgtccata cccattctat
     2761 aacaacctct ctatcccctt actcccccaa ctcagcactg taggcgataa tctggtcaac
     2821 tgtgtcagac aagaattgga tggtatcacg gagtggtttg tctgttgaaa tatcagcacc
     2881 gataatcatg gctgactcaa gtggtgtctt gctaccacct gatttgagga gattgagcca
     2941 gtcttcagct ccagtttctg agtttttaag atgaaggtaa ccagctgtcg agatgacaag
     3001 accagcagag taagtgtaac tatacaagcc catatagtag tgagcttggc gcatccaagt
     3061 caaagctgca tcgtcgtcga tttcaatggc atcgccccaa aaatctgtca aaacttcctt
     3121 cataatgctg ttgagtttgc ttgctccaaa ggtctcccct tcttcaatca atgtatacac
     3181 cttacgctgg aaggcggctt ccaagaggtg ggtgataaag ttatggaagt aggtgtctgt
     3241 caagcgatga gccagagcga agcgtttttg acgtgggtca ttagactggt tctctaagta
     3301 atcactgagt agcaattcat tgaaggttga cggtgcttca acataatagg tcgacatgtg
     3361 ggcgttgaaa tagctttggt gattgtctga aaagatgaat tgaccagaat gcccgatttc
     3421 atgaatcaag gtatagacat cgctcaaacg gcctgtccag ctcatgagta cataagggtg
     3481 tacgcgatat gggtccgccg cataaccacc ggaatccttg ccactgttag cagcaaagtc
     3541 cacccagcgc tcttcttggt aacgagcaac ttcctgacaa tattcttgcc ccaaaggttc
     3601 taccgacttc atgaccaaat cataggcatc gtcaatagtc acttcaggat tcagggcgct
     3661 gtccaagtcc aatttccagt ctgcaaaggt catcttttca agaccattta ccttggcaac
     3721 atgcttgagg tatctctgag cgactggtgc aaagtccttc atgatgaggt caatctggcg
     3781 gtcaaacatg acacggtcca cttcttgttc agctagaaga taatcaaata cagagtcgta
     3841 tcccttcata tcagccaaga gtttttcaga cttgacctga gccagatagg ctgctgcagc
     3901 cgtattttgg tgcttacgaa gtccctctga gaaggaacgg aaggatttct cacgaacctc
     3961 agcatcctcg tgattttggt agaaattctc ataggtgaca aagctgtttt tataggtctt
     4021 gccatgggct tcaaagtcag ccatttcaaa atccccagct cgcatcttag tataaatgtc
     4081 ctgcggacta tagaaaactt caccgagatt ggtcaaggcc ttctccacat cagcccctag
     4141 atagtgggct tttttgattt tggcctgacg aatagctgcc gtcaagtgag gcaattcacc
     4201 caaacggtcc aagacttcct cgtctgctac caccaaggca tcgtcaaaga aggtcaaggc
     4261 tacgctggca tctgtttcaa attccatccc agcttgggca atattggcaa attcgtcatt
     4321 gctatagtcc gtcgtctgag gcataaaagc atagttgcca atatggctca tctgaatgta
     4381 gatctgttct aattccgcaa aggccttctc gaaatcctca aaagtgtgaa gattgccctt
     4441 gtaatcacgg ctaaactggt tgatgtcttc acgagctttc tcgattgcac gcaagaaatc
     4501 ctcacggtct tggtataggg ctgttaagtc ccagagttcc ttctctggaa attctgaacg
     4561 gtgtttttgt tccattttct tcctcttatt tctctaattc tactaaaaca ctaagggctg
     4621 ataaggcgta aagcggtgct gtttctgctc gcaaaatacg aggacctagg cctaccaaaa
     4681 cggctccctt agcttcaaaa ctttcgattt ctgcaggtga gagaccgcct tctggaccaa
     4741 agataaagag cagtttggct cctgtttcaa gaccagtgac tgcttgcaga agcgcagcgg
     4801 cttctccttc tttagctgat tcttcatagg ctactatgat aaagtcaaac tggtccagct
     4861 gagctagaaa atctgctttt ttctcgaaaa gtttaatact tggtacaata ttacgcttgc
     4921 tttgctcggc tgctccaagg gcaatttttt ctagtttttc aactttttta cccaatttct
     4981 tgccatccca cttggcaact gaccagtctg caggaaaggc ccagatttgg ctagcaccca
     5041 gttcggttac tttttgagcg atgaactcca gcttgtctcc cttgggaaat ccagatgcga
     5101 tggtcacttg gactggtagt tccacattgt catttaattc ttggaccaac tcaaactgac
     5161 gtttttccat atccagcacg cgcgccaagc gcttgatgcc atcatcaaag actaaggtaa
     5221 cctcatcctc ttctttcaag cgcataacct gaaacatatg cttactggtt tccttgtcct
     5281 cgatagtgac aggagagata gcactgcctt ttacaaaata ctgctgcatg ctagcctcca
     5341 atcacaccag agatatcctt ggttttctta aagacacagg tattccattc cccttgaacc
     5401 atgtgagttt cgaggaaaaa tccagctgac tcagccgact ggcgcaccat gtcccacttg
     5461 tccttgataa tgccactcat gatcaggtag ccttcgtcct taaccaaacg ataggcatcg
     5521 tctattagat gaatgaggat atccgccaag atattagcca caatcacatc tgcctcaatt
     5581 tccacaccct taagcaaatc tccagccgct acatggatat tttccatgcc agggttgagc
     5641 tcaatatttt cctgagccac acgaaccgcc acatcatcca ggtcataggc gaaaatttct
     5701 ttagccccca gaagcgagct ggcaatagag agaacccctg aaccagtccc cacatctagc
     5761 accgtttcgc caccacgaag aacctgttcc aaggcaaaaa ggctcatctt ggtagttggg
     5821 tgggttccag taccaaaagc catgccagga tccagcttga taattttttc tcccgcagtc
     5881 gcctcatagt ctgtccaaga gggaacgatg gtcaaatcat gagtgatacg agctggctca
     5941 tagtatttct tccagttgtc tgcccagtct tcctcagcca aggcagtcgt acctattttt
     6001 aactctccca aatccataaa atccgtcaat tctgatatgc gagcctgcaa gtccgcctca
     6061 accactgtca catccaccgt gtcagggtag taggctgtca caacgatttc ttcttgctgc
     6121 tccacctctg ggaaaatctc gccaaagcgg tcaacatttc ccacatagtc catactgtct
     6181 tcgattgcga ctccttgcgc tcccagctca atcaagagat tggaaaccaa ctcctctccc
     6241 tcacgcttca ctgtaacttt taactcttgc catgtttcca ttattaagat accaagcccg
     6301 taaaacacaa aaccaaaata ggaaattctc tgaagacgct tgtgtctaag agaagtttat
     6361 ctttttggca cagtgtttag ggcgggttca gtttagaaat ttaactgaac catcctttct
     6421 aatcacttac ttttaaataa tcttttaatc tctcttgcaa ctgagggaca acttgactgg
     6481 aactaagaaa ttcctcaaca ttcatcagct gatagccctg tccttcatct ccaaatacga
     6541 tactgtcaaa ctggttctgt gtcaactgac caactagaaa aacggactgt ttgtctgcaa
     6601 aaagcatact tgggtaaacc ttactccaaa gcagacaatc ttcagtcaga tgaatgccca
     6661 gttcttcata aacttcacgc gctacacact caaaaggact ttcatcacct tcacgtccac
     6721 cacctggcag ttcccacata ttggcccagg gaatacttgc cttatcatca cgtaagatag
     6781 tcaagagctt atccccacaa aacaaagcaa tcttacagcc tgggaaatca gaaatttcta
     6841 gttccatctt cagttccttc taacatttcc ttttccagct ctgctaacaa gttttcataa
     6901 tagcgtttct cgtccctcaa cattcgacta ctatccattt tctgtctagc aatcttgaga
     6961 gccttacgag ttcgatctac atctttcttt accttaaatt gataccaagc ttgtatcact
     7021 tgaagattgg acagtttgag agacagaaac gatttgacct gtcgaatact agcatattgc
     7081 tccgcttgct caaaatctcc ttccaacaag gcgatatgaa gcagggatag ttgggcaact
     7141 gtctgcatca tcggagtagt tgtcctctca agtaatgctt gaaactgctg tttagctact
     7201 tcttccttcc cttccaaaat ggaaacttca ccttgcatac ctaatacacc atccgcaaaa
     7261 ctccctcgtg catcctcagg aactgcttga acaaagtctt tcaaatcata ttcttgagga
     7321 gccagcaaag tttgagcaga atgtctcaat accaggtagg cgtatttggt attttcaggg
     7381 tgttgtagta attcccaaat ttttgctcca tcggtgatgt cgactggcaa aatgttattt
     7441 aggaagaaag ataaattaag aaaaatccaa gtccctgcaa aataccagct tcttgtcaaa
     7501 aatccaaaca ctatcgccaa taatatcaag ccgagatgaa ccatcaagcc tcctgaaagc
     7561 atcaggatga ttctttgatc gctttcatcc tcttttaaac caatgtattg agcaccaaca
     7621 tttttcagaa tggctgttct actaagatga aacctgcctg actttttggt caaaataaaa
     7681 tgtcctaatc caaaagccac cagccgatag cctgtcaagt agccacaaaa agcatgaccc
     7741 agctcatgaa gaataaagat taaatacatg cttagaagag cgaaggcata accaaaagta
     7801 aaggctaaaa ctgcggaata ccccaactct gcaaatgcga ttgttccaca agcaaaagct
     7861 agcataataa agacaaaagc tagcacataa accaaataag tcccaatttt cttcataaca
     7921 cctccaacca actcctagta tcttggataa ggataaaatt ctccctcttc caagccaact
     7981 tttccttctt caaagacttc ttggttccat tccatgacaa attcctctgc ttctgggtct
     8041 tccaaaaagt ccatgaggac atctagccca acctcagcag tatctttaag gaaaagcgca
     8101 aaataagcta aaaattcacg ggaaaatcct tttttaggca ggtaaggaat aacagtcaaa
     8161 tagtcttcct cattgactgt tgacttggca ggattgtaga aaaggaccgc ttcctcaaaa
     8221 agaatgtcat ctgataaaac ctctccgtct tcatccacca tctccacacc tgcagcattt
     8281 tgcgcttcca atagaaaact cacttctacc gcatggttgc gtttgtccca gctaatctca
     8341 aagtcaaagg gaaagttctt gtccaactct tcctctaaaa catctaaaaa tccgtatgtt
     8401 gccattttgt cctctttcta tgcgactctt taatcgcccc gattgctcgg aaatatgcta
     8461 aaatagatac taccatctta ccacaaaatt attttatgtc ctaattatac catattacct
     8521 catttaaacc cttggtatca gtgattttct taaaagtctg atttcttcat ttctcataaa
     8581 aatcaatata aaaagcccgc gaaagggcta ataaatctat aaaatcaata ggcgagtaac
     8641 tagcacaagt ggacgtgctt ttttattgac tattaccacg ataccacgct taatcttagg
     8701 cttgaacttt cttatctgca atagcgtctg tcaaagtctg agaaaagtta agtcccattt
     8761 ctcgtcccaa cttatctgcc cattttggta tggtcaaagt cttttttatg ggttcctgac
     8821 ttcctaggta ttctgataca tcaacagata ccatagaaat aaaagattta tcaaggtcat
     8881 aggttgacac gaaatcttca tcatctttaa aaggatcatt atcaattaaa gacaagctat
     8941 tgatatctga tggctgaggt aactctccat cactctctat caaatctgca acagttatcc
     9001 ctagccactc cgaccccata gccaaagcct cagaaatccc ctctccttgt gtagctgagt
     9061 attcaaaatc tgggaaatgg acaaaataag tcgcttccgt tccgtctgtg tcgtcataat
     9121 aaaataaagc tggatacgta actaacattt cactacctcc atatcaaaaa acaggggctg
     9181 aattttacaa cccagcttgc tttcttatcc ctctttcagt gtacttattc agctcaccat
     9241 gagggattgt gataagtctt tccccttgct tctccatttt aatatgggag cctttaccgc
     9301 ctctagtctt tatccaacca tgggccgtaa ggagtttaac catctctttt tgtgtcatag
     9361 gcatagcgct tttacctcct gacaacacca ttataacacg tgttacacgt attgtaaagg
     9421 agtgatactt attattctat tatacataaa agcccctaga tgtggttcta agggaagcca
     9481 atttattcag acctattttt ctaatgagta gtaaaaactg cttctttatc gagcaattca
     9541 tcatctgtat agtcaattgt aaaagtatct cgatctaaga cagattgagg cggagttgaa
     9601 tgaatcatag gaacactgcg tactctatat tttttatctc caatttttac aaactgatac
     9661 tcttcgaaaa tcaaattcaa accacgtcaa cgtcgcctta ccgtactcaa gtacagcctg
     9721 cggctagttt cctagtttgc tctttgattt tcattgagta tgattaactc tcaa
//
LOCUS       DAVLFB010000081         9562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLFB010000081 DAVLFB010000000
VERSION     DAVLFB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.081"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            198..596
                     /locus_tag="VXD58_001666"
     CDS             198..596
                     /locus_tag="VXD58_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676329.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            1041..2048
                     /locus_tag="VXD58_001667"
                     /pseudo
     CDS             1041..2048
                     /locus_tag="VXD58_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2123..2323
                     /locus_tag="VXD58_001668"
     CDS             2123..2323
                     /locus_tag="VXD58_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV0676330.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            2358..3074
                     /gene="atpB"
                     /locus_tag="VXD58_001669"
     CDS             2358..3074
                     /gene="atpB"
                     /locus_tag="VXD58_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV0676331.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            3088..3582
                     /gene="atpF"
                     /locus_tag="VXD58_001670"
     CDS             3088..3582
                     /gene="atpF"
                     /locus_tag="VXD58_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV0676332.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            3582..4118
                     /locus_tag="VXD58_001671"
     CDS             3582..4118
                     /locus_tag="VXD58_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV0676333.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            4133..5638
                     /locus_tag="VXD58_001672"
     CDS             4133..5638
                     /locus_tag="VXD58_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV0676334.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            5654..6532
                     /locus_tag="VXD58_001673"
     CDS             5654..6532
                     /locus_tag="VXD58_001673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV0676335.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            6618..8024
                     /gene="atpD"
                     /locus_tag="VXD58_001674"
     CDS             6618..8024
                     /gene="atpD"
                     /locus_tag="VXD58_001674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV0676336.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            8035..8454
                     /locus_tag="VXD58_001675"
     CDS             8035..8454
                     /locus_tag="VXD58_001675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV0676337.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(8526..9562)
                     /locus_tag="VXD58_001676"
                     /pseudo
     CDS             complement(8526..9562)
                     /locus_tag="VXD58_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2954 a         1735 c         2072 g         2801 t
ORIGIN      
        1 tatctacaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctctt
       61 tgatttttat tgagtattag atgtatagtg cattgaagtt aaaatagtac actttgattt
      121 ctaagacatt gttagaaatt agattaaatt ccttaatcga tttgcgcatg ttttatttca
      181 tttcactata ctttagattg gaaaataagg aatcacataa atcgcaagaa atagggaatc
      241 tgattcgtgt ttataaccgt tcaaaaagag aagaggctga aagtgagcca cttaatcttt
      301 atgtcgaaga tgaaaagggc aatctcctgg caggtttgat agcagagact tttggaaatt
      361 ggctagaaat cgagtatttg tttgtaaaag aggaactgcg agggcaagga atcggttcaa
      421 aactattgca acaggcagaa agtgaagcta agaatcgaaa ttgttgtttt gcctttgtca
      481 atacttacca gttccaggca ccggactttt atcagaaaca tggctacaag gaagtttttt
      541 ctttgcaaga ctatctctac attaggcaaa gatattatta ccaaaagaat ctgtaaggtg
      601 agtatggagg tataatacaa agagaggctc gcacataagt atggaggtgg actgatgtat
      661 atgagtaact atcgataaat accagttata acctagaaat tgtgtctttc tagacgatat
      721 tgaggacaat ataatcgtag ccccaaaatt aggaatcaag acttatcagg ttaagaaaag
      781 aagtgatgtt gttgatattt taaacaattt atttaaacta aaaactctct atctattaat
      841 ccgagattcc cctctcggat tttgtgttgc tttcattaaa tttacaggta agacttgcta
      901 gcttcagtaa atatacacaa gtttttcaga atagagtgaa atccaaaaga aaatggaaat
      961 aatgaaagct cttgtaggca taagaaaaca cctctgtgtt atacttgttg ttcaaccaca
     1021 aacacaagaa aggcacagag atgcaagaca attatactac aaaagccaaa catttgacaa
     1081 tcgatagccg tcgcttaatc gaaagatgga aaaaagaagg gaaatcaaat agataaattg
     1141 cctctctgct tggaaaagtt cctcaaacta tccacactga aatcaagcgt gggacagtcc
     1201 gacaatgtct tggaaaaggg cgcttcaaag aggtttattc tgccgactat gctcaacagt
     1261 cttatgaaaa taatcgcaag cgctcggtca agaaatcaag cttgaccaag gaactaaagg
     1321 aaaagattct ccactatcat aaccaaaaat tttcgcctga aatgatggtg atggctaaag
     1381 gggttaacgt gggaatttca accatttact attggattca tcatggaaaa ttggggttaa
     1441 gcaaacagga tctgctttac cctagaaaag gaaaagcgct taagaaacag gctagcacca
     1501 actttaaacc tgctggtcaa tccatcgaac agcggtctga agctatcaat cttcgcttgg
     1561 agaatgggca ttatgagatt gatacggttc tacttacgag agcgaaaaac tactgcttgc
     1621 ttgtcttgac ggatcgaaag agtagacatc agatcatccg attgattcca aataaaagtg
     1681 ctgaggtggt caatcaggct ctaaaactca tcttaaaaca acacaagatt ctttccatca
     1741 cggcagataa tggaacggaa ttcaatcgct tgtttgatgt attttctgag gagcacatct
     1801 attatgcgca cccctatgcc tcttgggaaa ggggaactaa tgagaatcac aacaggctca
     1861 ttcgtagatg gttacctaag ggaaccaaga aaatgactcc caaagaagtc gcattcatcg
     1921 aaaagtggat taacaactat cctaaaaaat gcttggacta caagtcaccc agagaagact
     1981 tctggatggc taacttgaac ttgaaattta gcaaaatgga aataattttt attaaacgct
     2041 tccaatagcg gttaaaagtt gacaaattta aattttagga ctacactaag taagtaaatt
     2101 ttattttaaa ggagaatttg ttatgaattt aacattttta ggcttatgta ttgcctgtat
     2161 gggcgtatct gtcggtgaag gtttattgat gaatggactg tttaaatcag tagcacgcca
     2221 accagatatg ctttctgagt ttcgtagttt gatgttttta ggtgttgcct ttattgaagg
     2281 aactttcttt gtaactcttg tcttctcatt tattatcaaa taaatacatg gaacgagaag
     2341 aaaagggagg attttagatg gaagaaagta ttaatccaat catctctatt ggtcctgtta
     2401 tcttcaatct gactatgtta gccatgactt tgttgattgt gggagttatt tttgtcttta
     2461 tttattgggc aagccgcaat atgaccttga aacccaaagg aaagcaaaat gtacttgagt
     2521 atgtctatga ctttgttatt ggatttacag aacctaacat tggttcgcgc tacatgaaag
     2581 attactcact ctttttcctt tgtttattcc ttttcatggt gattgccaat aaccttggct
     2641 taatgacaaa gcttcaaacg atcgatggga ctaactggtg gagttcgcca accgctaatt
     2701 tacagtatga cttaacctta tcttttcttg tcattttgtt gacacatata gaaagcgttc
     2761 gtcgtcgtgg atttaaaaaa agtataaaat cttttatgag tcctgttttt gtcataccga
     2821 tgaatatctt ggaagaattt acaaacttct tatctttggc tttgcggatt tttgggaata
     2881 tctttgcagg agaggtcatg acgagtttgt tacttcttct ttcccaccaa gctatttatt
     2941 ggtatccagt agcctttgga gctaatttgg cttggactgc attttctgtc tttatttcct
     3001 gcatccaagc ttatgttttt actcttttga catctgtgta tttagggaat aagattaata
     3061 ttgaagagga atagaaagga gtaactgatg cacgtaacag taggtgaatt aattggtaat
     3121 tttattttaa tcactggctc ttttattctt ttgctagtct tgattaaaaa atttgcatgg
     3181 tctaatatta caggcatttt cgaagaaaga gctgaaaaaa ttgcttcaga tattgacaga
     3241 gctgaagaag cccgtcaaaa agcagaagta ttggctcaaa aacgcgaaga tgaattggct
     3301 ggtagccgta aagaagctaa gacaatcatt gaaaatgcaa aggaaacagc tgagcaaagt
     3361 aaggctaata tcttagcaga tgctaaacta gaagcaggac acttaaaaga aaaagccaat
     3421 caagaaattg ctcaaaataa agtagaagct ttacagagtg ttaagggtga ggtcgcagat
     3481 ttgaccatca gcttagctgg taaaatcatc tcacaaaacc ttgacagtca tgcccataaa
     3541 gcactcattg atcagtatat cgatcagcta ggagaagctt aatggacaag aaaacagtaa
     3601 aggtaattga aaaatacagc atgccttttg tccaattggt acttgaaaaa ggagaagaag
     3661 accgtatctt ttcagacttg actcaaatca agcaagttgt tgaaaaaaca ggtctgcctt
     3721 cttttttaaa acaagtggca gtagacgagt cggataagga aaaaacaatt gcttttttcc
     3781 aagattctgt gtcgccttta ttacaaaact ttatccaggt tctggcctac aatcacagag
     3841 caaatctttt ttatgatgtg cttgtagatt gcttgaaccg acttgaaaaa gaaacaaatc
     3901 gatttgaagt gacgattacg tctgctcatc ctctaactga tgaacagaag actcgtttgc
     3961 tccctttgat tgagaaaaaa atgtctctga aagtaaggag tgtaaaagaa caaatcgatg
     4021 aaagtctcat tggtggtttt gtcatttttg ccaatcacaa gacaattgat gtgagtatta
     4081 aacaacaact taaagttgtt aaagaaaatt tgaaatagaa agtggtgttc ttttggcaat
     4141 taacgcacaa gaaatcagcg ctttaattaa gcaacaaatt gaaaatttca aacccaattt
     4201 tgatgtgact gaaacaggtg ttgtaaccta tatcggggac ggtatcgcgc gtgctcatgg
     4261 ccttgaaaat gtcatgagtg gagagttgtt gaattttgaa aacggctctt atggtatggc
     4321 tcaaaacttg gagtcaacag acgttggtat tatcatccta ggtgacttta cagatatccg
     4381 tgaaggcgat acaatccgcc gtacagggaa aatcatggaa gtccctgtag gtgaaagtct
     4441 gattggtcgt gttgtggatc cgcttggtcg tccagttgac ggtcttggag aaatccacac
     4501 tgataaaact cgtccagtag aagcaccagc tcctggtgtt atgcaacgta agtctgtttc
     4561 agaaccattg caaactggtt tgaaagctat tgacgccctt gtaccgattg gtcgtggtca
     4621 acgtgagttg attatcggtg accgtcagac agggaaaaca accattgcga ttgatacaat
     4681 cttgaaccaa aaagatcaag atatgatctg tatctacgtc gcgattggac aaaaagaatc
     4741 aacagttcgt acgcaagtag aaacacttcg tcagtacggt gccttggact acacaatcgt
     4801 tgtgacagcc tctgcttcac aaccatctcc attgctcttc ctagctcctt atactggggt
     4861 tgctatggcg gaagaattta tgtatcaagg taagcatgtt ttgattgtat acgatgatct
     4921 atcaaaacaa gcggtagctt atcgtgaact atcgctcttg cttcgtcgtc ctccaggtcg
     4981 tgaagccttc ccaggggatg ttttctatct ccacagccgt ttgcttgagc gctcagctaa
     5041 agtttctgat gaacttggtg gtggatcaat tacagcccta ccatttatcg agacacaagc
     5101 aggagatatc tcagcctata tcgcaaccaa cgtgatttct atcactgatg gacaaatctt
     5161 ccttggcgat ggcctcttca atgcaggtat tcgtccagcc atcgatgcgg gttcatctgt
     5221 atctcgtgta ggtggttctg cacaaatcaa agccatgaag aaggttgctg gtacacttcg
     5281 tatcgacctt gcttcatacc gtgagttgga agcctttact aagtttggtt ctgacttgga
     5341 cgcagcaaca caggctaagt tgaaccgtgg acgtcgtacc gttgaggtct tgaaacaacc
     5401 tgttcacaaa ccattacctg ttgagaaaca agtaaccatt ctttatgctt tgacacatgg
     5461 tttcttggat actgttccag tagatgatat tgttcgtttc gaggaagagt tccatgcctt
     5521 ctttgatgct caacatccag agattttgga aaccattcgt gatacaaaag acttgccaga
     5581 agaagcagtc ttggatgctg cgattacaga gtttctcaat caatctagct tccaataaga
     5641 atagaggtgt cagatggcag tgtctctaaa tgatattaaa acaaaaatcg cctcaacaaa
     5701 aaatacgagt caaatcacta atgccatgca aatggtatcg gctgctaagt taggtcgctc
     5761 tgaagaagct gctcgcaact tccaagttta cgctcagaaa gtgcgtaaac ttttgacaga
     5821 tatccttcat ggtaatggag ctggtgcttc aactaatccg atgttgatta gccgttctgt
     5881 gaagaagaca ggctatatcg ttatcacttc agaccgcggt ttggttggag gttataattc
     5941 ctctattttg aaagctgtta tggagttgaa agaagaatac cacccagacg gtaaaggttt
     6001 tgaaatgatc tgtatcggtg ggatgggagc tgatttcttt aaggctcgcg gtattcaacc
     6061 actttatgaa ttacgtggct tggcagacca acctagcttt gatcaagttc gtaagattat
     6121 ttcaaaaact gttgaaatgt accaaaatga actctttgat gagctttatg tttgctacaa
     6181 ccaccatgtc aatacgctaa ccagtcaaat gcgtgtggaa caaatgcttc cgattgttga
     6241 cttggatcca aatgaagcgg atgaagagta cagcttgact tttgaattgg aaaccagccg
     6301 agaagaaatt ctggagcagt tgttgcctca gtttgcagaa agtatgattt acggtgccat
     6361 tatcgatgcc aagacagctg agaatgctgc gggtatgaca gccatgcaaa cagcgacaga
     6421 taatgctaag aaagtcatca atgatttgac aattcagtat aaccgtgcca gacaggcggc
     6481 gattacacaa gaaattacag aaatcgtagc aggtgctagt gccttagaat aggctctagt
     6541 ccagctcgta tgaaaatgaa cttaggacct agttgagcta ggaaccgaca gtatcttata
     6601 tagaatagga gaaggagatg agttcaggta aaattgctca ggttatcggt cccgttgtag
     6661 acgttttgtt tgcagcaggg gaaaaacttc ctgagattaa caatgcactt gtcgtctaca
     6721 aaaatgacga aagaaaaaca aaaatcgtcc ttgaagtagc cttggagtta ggagatggta
     6781 tggttcgtac tatcgccatg gaatcaacag atgggttgac tcgtggaatg gaagtattgg
     6841 acacaggtcg tccaatctct gtaccagtag gtaaagaaac tttgggacgt gtcttcaacg
     6901 ttttgggaga taccattgat ttggaagctc cttttacaga agacgcagag cgtcagccaa
     6961 ttcataaaaa agctccaact tttgatgagt tgtctacctc ttctgaaatc cttgaaacag
     7021 ggatcaaggt tattgacctt cttgcccctt accttaaagg tggtaaagtt ggacttttcg
     7081 gtggtgccgg agttggtaaa accgtcttaa tccaagaatt gattcacaac attgcccaag
     7141 agcacggtgg tatttcagta tttactggtg ttggggaacg tactcgtgag gggaatgacc
     7201 tttactggga aatgaaagaa tcaggcgtta tcgagaaaac agccatggtc tttggtcaga
     7261 tgaatgagcc accaggagca cgtatgcgtg ttgcccttac tggtttgaca atcgctgaat
     7321 acttccgtga tgtggaaggc caagacgtgc ttctctttat cgataatatc ttccgtttca
     7381 ctcaggctgg ttcagaagta tctgcccttt tgggtcgtat gccatcagcc gttggttacc
     7441 aaccaacact tgctacggaa atgggtcaat tgcaagaacg tatcacatca accaagaagg
     7501 gttctgtaac ctctatccag gctatctatg tgccagcgga tgactatact gacccagcgc
     7561 cagcaacagc cttcgctcac ttggattcaa caacaaactt ggaacgtaaa ttggtacaat
     7621 tgggtatcta cccagccgtt gacccacttg cttcaagctc acgtgccttg gcacctgaaa
     7681 tcgttggaga agagcactat gcagttgctg ctgaagtaaa acgtgtcctt caacgttacc
     7741 atgaattgca agatatcatt gctatccttg gtatggatga gctttctgat gaagaaaaga
     7801 ccttggttgc tcgcgcccgt cgtatccagt tcttcttgtc acaaaacttc aacgttgcgg
     7861 aacaatttac tggtcagcca ggttcttatg ttccagttgc tgaaactgta cgtggcttta
     7921 aggaaatcct tgatggtaaa tacgaccact tgccagaaga tgccttccgt ggtgtaggtt
     7981 ctatcgaaga tgtgattgca aaagctgaaa aaatgggatt ttaagaggtg acctatggct
     8041 cagttaactg tccagatcgt gacaccagat ggtctcgtct atgatcacca tgccagctat
     8101 gtatcggttc gaactctgga tggtgagatg gggatcttgc cacgacatga aaatatgatt
     8161 gcggttttag cagttgatga agtaaaggta aaacgtatcg atgataaaga tcacgtgaac
     8221 tggattgcag taaacggtgg cgttattgaa attgccaatg atatgatcac aatcgtcgct
     8281 gactctgcag aacgtgctcg tgatatcgat attagccgtg cagaacgtgc caaacttcgt
     8341 gcagaacgtg caattgaaga agcacaagac aaacatttga ttgaccaaga acgtcgtgct
     8401 aagattgctt tgcaacgtgc cattaaccgt attaatgtcg gaaatagact ataagaaaaa
     8461 atgaacttga aaataccaag ttcatttttt aggctctttg tcaactgtag tgggttgaaa
     8521 aaaagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcgata
     8581 aaaatccgtt ttttgaagtt ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt
     8641 ttgatgagat tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg
     8701 acgattttct ctttgtcctt tagaaaggtt ttaaagacag tctgaaaaat aggatgaacc
     8761 tgcttaagat tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctgaaagtga
     8821 aacagcaaga gttgatagag ctgatagtgg tgtttcaagt cttgtgaata gctcaaaagc
     8881 ttgtctaaaa tctctttatt ggttaaatgc atacgaaaag tagggcgata aaaatgttta
     8941 tcgctgagtt tacgactatc ctgttgaatg agtttccagt agcgcttgat agccttgtat
     9001 tcatgggatt ttcgatgaaa ctgattcatg atttgaacac gcacacgact catagcacgg
     9061 ctaagatgtt gtacaatgtg aaagcgatca agaacgattt tagcattcgg gagtgaaaca
     9121 gtctgggaga ctgtttcagc ctgagcctag aaatttgaaa gcgaagctgt ttagccaagt
     9181 catagtaagg gctaaacata tccatagtaa tgattttgac gcgacatcgg acagctctat
     9241 cgtagtgaag aaagtgattt cgaatgatag cttgtgttct accctcaaga acagtgatga
     9301 tattgagatt gttaaaatct tgcgcaatga agctcatctt tccctttgta aaagcatact
     9361 catcccaaga cataatctca ggaagacaag aaaaatcatg tttaaagtga aaatcattga
     9421 gcttacgaat aacagttgaa gttgaaatgg ccagctgatg agcaatatcg gtcatagaaa
     9481 tcttctcaat caacttttgc gcaatttttt ggttgatgat acgagggatt tggtgattct
     9541 tcttgacgat agaagtttca gc
//
LOCUS       DAVLFB010000082         9482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLFB010000082 DAVLFB010000000
VERSION     DAVLFB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.082"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            39..1712
                     /locus_tag="VXD58_001677"
     CDS             39..1712
                     /locus_tag="VXD58_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV0676338.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINKEIVVQAFLEDI"
     gene            1824..2915
                     /gene="ulaG"
                     /locus_tag="VXD58_001678"
     CDS             1824..2915
                     /gene="ulaG"
                     /locus_tag="VXD58_001678"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV0676339.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3029..5005
                     /gene="tkt"
                     /locus_tag="VXD58_001679"
     CDS             3029..5005
                     /gene="tkt"
                     /locus_tag="VXD58_001679"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV0676340.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            5122..5421
                     /gene="yajC"
                     /locus_tag="VXD58_001680"
     CDS             5122..5421
                     /gene="yajC"
                     /locus_tag="VXD58_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV0676341.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5468..5896
                     /locus_tag="VXD58_001681"
     CDS             5468..5896
                     /locus_tag="VXD58_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV0676342.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLCQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5898..6308
                     /locus_tag="VXD58_001682"
     CDS             5898..6308
                     /locus_tag="VXD58_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676343.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6608..9259
                     /gene="adhE"
                     /locus_tag="VXD58_001683"
                     /gene_synonym="adhC"
     CDS             6608..9259
                     /gene="adhE"
                     /locus_tag="VXD58_001683"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV0676344.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3030 a         1748 c         1979 g         2725 t
ORIGIN      
        1 attgctgtga tatcaataga tttgggggat tttttaatat ggtactggat aaggcaagtt
       61 gtgatttgct tcaatatttg atggatcaag aaacgtccaa aacgattatg gcgatttcga
      121 aagatttgaa agagtcaaga aggaaaattt attatcacat tgacaaaatc aatgctgctc
      181 tggttgacga ggcgcttcac atcattagta ttccacgaat tggtattcac ttaacggaag
      241 agcagagaga tgcttgttgt aaactattat cggaagtaga ttcgtacgat tatatcatga
      301 gtgcgcatga acgtatgatg ataatgttac tatggatagg tatttctaaa gaacgtatta
      361 cgattgaaaa attgatagag ttaacagagg tatctaggaa tactgttctc aatgatttga
      421 atagtattcg ttatcaacta actttggaac aatatcaggt gatcttgcaa gtgagcaagt
      481 cacagggata ccaccttcat gcccaccctc ttaataaaat tcagtatctt caatcgcttc
      541 tatatcatat ttttatggaa gaaaatgcca cttttgtatc tattttagaa gataagatga
      601 aagagaggtt agatgatgag tgtttgcttt ctgttgaaat gaaccaattt tttaaggaac
      661 aggttccttt agttgaacaa gatttaggga agaaaataaa ccatcatgaa ataactttta
      721 tgttgcaggt tctaccttat ttgctgttaa gctgtcataa tgttgaacag tatcaagaaa
      781 gacatcagga tatagagaaa gaattttctt tgataagaaa aagaatagag tatcaggtgt
      841 ctaagaaatt aggagaacgg ttgtttcaaa agtttgaaat ttctttgtca ggacttgaag
      901 tttctcttgt agctgttctc ctcctctcct atcgtaagga tttggatatt catgcagaaa
      961 gtgatgattt tcggcaatta aaacttgctt tagaagaatt tatctggtat tttgaatcac
     1021 aaatccgaat ggagattgag aacaaggatg atttgttacg aaatttgatg atccactgta
     1081 aagccttgtt atttagaaag acttacggta ttttttctaa aaatcctcta acaaaacaaa
     1141 ttcgatccaa gtatggagaa ttatttttag tcactagaaa atctgcggaa attttagaag
     1201 gagcatggtt tattcggcta acagacgatg atattgccta tttgacgatt catattggag
     1261 gatttttaaa gtatacacca tcgtctcaaa aaaatatgaa aaaagtttat ctcgtttgtg
     1321 atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg caaactttat tttccaaatg
     1381 agcaaattga cactgtattt acaacagaac aatttaagag tgtggaagat attgcacaag
     1441 ttgatgtagt gattactact aatgatgatt tggatagcag atttccgatt ttaagggtta
     1501 atcctatcct tgaagcagaa gatattttga aaatgctaga ctatcttaaa cacaatatat
     1561 ttcgtaataa gagcaaaagt ttcagtgaaa atctttctag tcttatttcg tcttatattg
     1621 tagacagcaa gttggctagt aagttccaag aagaggttca aacacttata aataaagaaa
     1681 tagtagttca agcttttttg gaagatattt gaaggacagt ccaatgatga acacaaacct
     1741 gtgtttttct tggtcttttt tagtgttttg aagggtggta tactaatctc aaagataaca
     1801 attatatcca aaggaggcaa catatgccaa acgtcaaaga aattacaaga gagtcatgga
     1861 ttttagccac tttcccagag tggggaacat ggttgaacga agaaatcgaa gaagaagtcg
     1921 tacctgaagg caactttgcc atgtggtggc taggcaactg tggtacttgg attaagacac
     1981 cagctggtgc taacgttgtc atggaccttt ggtcaaaccg tggaaaatca accaaaaaag
     2041 tgaaagatat ggttcgtggg caccaaatgg caaatatggc aggtgttcgt aagttgcaac
     2101 caaacttgcg tgttcagcca atggttatcg atccatttgc tatcaacgaa ctagactatt
     2161 acttagtttc acacttccac agtgatcata tcgacccata cacagctgca gcaattctca
     2221 acaatcctaa gttagagcat gttaagttta tcggtcctta ccactgtgga cgaatctggg
     2281 aaggatgggg tgttccaaaa gaacgtatca tcgttgttaa accaggtgac actatcgaat
     2341 taaaagatat gaagattcat gcagtagaat catttgaccg tacttgcttg gtaactctcc
     2401 cagtgaacgg tgctgatgag acaggcggtg aacttgctgg cttggctgtt acagatgaag
     2461 aaatggctca aaaggctgtt aactatatct ttgaaacacc aggtggaacc atctatcatg
     2521 gtgcagattc tcacttctca aactattttg caaaacatgg taaagacttt aaaattgatg
     2581 ttgctttgaa taactatggt gaaaatccgg taggtatcca agacaaaatg acatctatcg
     2641 atcttcttcg tatggcagaa aatctgcgta ccaaagtcat tatcccagtt cactatgata
     2701 tctggtctaa cttcatggct tctactaatg agattctaga actttggaaa atgcgaaaag
     2761 atcgcttgca atacgatttc catccattta tctgggaagt tggcggtaag tacacttatc
     2821 ctcaagatca acacttagta gaataccatc atccacgtgg ttttgatgat tgttttgaac
     2881 aagactctaa cattcaattt aaagctttgc tataatataa aaatattctt tggaggttat
     2941 ccgtatgttg catgatacgg ataattttca tataatagta aaatagaatg tgtgattcaa
     3001 taatcacctc aaatagaaag gaaattctat gtcaaatcta tctgttaatg caattcgttt
     3061 tctaggtatt gacgccatta ataaagccaa ctcaggtcat ccaggtgtgg ttatgggagc
     3121 ggctccgatg gcttacagcc tctttacaaa acaacttcat atcaatccag ctcaaccaaa
     3181 ttggattaac cgcgaccgct ttattctttc agcaggtcat ggttcaatgc tcctttatgc
     3241 tcttcttcac ctttctggtt ttgaagatgt cagcatggat gagattaaga gtttccgtca
     3301 atggggttca aaaacaccag gtcacccaga atttggtcat acggcaggga ttgatgctac
     3361 gacaggtcct ctagggcaag ggatttcaac tgctactggt tttgcccaag cagaacgttt
     3421 cttggcagcc aaatataacc gtgaaggcta caatatcttt gaccactata cttacgttat
     3481 ctgtggagac ggagacttga tggaaggtgt ctcaagcgag gcagcttcat acgcaggttt
     3541 gcaaaaactt gataagttgg ttgttcttta tgattcaaat gatatcaact tggatggtga
     3601 gacaaaggat tcctttacag aaagtgttcg tgaccgttac aatgcctacg gttggcatac
     3661 tgccttggtt gaaaatggaa cagacttgga agccatccat gctgctatcg aaacagcaaa
     3721 agcttcaggc aagccatctt taattgaagt gaagacggtt attggatatg gttctccaaa
     3781 caaacaagga actaatgctg tacacggcgc ccctcttgga gcagatgaaa ccgcatcaac
     3841 tcgccaagcc ctcggttggg actacgaacc atttgaaatt ccagaacaag tatatgctga
     3901 tttcaaagaa catgttgcag accgtggcgc atcagcttat caagcttgga ctaaattagt
     3961 tgcagattat aaagaagctc atccagaact ggctgcagaa gtagaagcca tcatcgacgg
     4021 acgtgatcca gtcgaagtga ctccagcaga cttcccagct ttagaaaatg gtttttctca
     4081 agcaactcgt aattcaagtc aagatgcctt gaatgttgtg gcagctaaat taccaacctt
     4141 cctaggtgga tcagctgacc ttgctcactc aaacatgact tatatcaaga cggatggact
     4201 tcaagacgat accaatcgct tgaatcgtaa cattcagttt ggtgttcgtg aatttgcaat
     4261 gggaacaatc ttgaacggga tggcccttca tggtggactt cgtgtatacg gtggaacttt
     4321 cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg tcagccttac aaggacttcc
     4381 tgtgacttat gtctttaccc atgattcaat cgcagttggg gaagatggtc cgactcacga
     4441 accagttgag catttagcag gtcttcgtgc tatgccaaat ctaaatgttt tccgtccagc
     4501 agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg acaagtgaga aaacaccaac
     4561 tgcccttgtc ttgacacgtc aaaacttgac tgttgaagat ggaacagact tcgacaaggt
     4621 tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac tttgatacca tcttgattgc
     4681 gacaggttca gaggttaatc ttgctgtctc agctgctaaa gaattggcta gtcaaggcga
     4741 aaaaatccgc gtagtcagca tgccatctac agatgtcttt gataaacaag atgcagctta
     4801 caaggaagag attcttccaa atgcagtccg ccgtcgtgtt gcagtcgaaa tgggtgcaag
     4861 tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt ctaggtattg ataccttcgg
     4921 agcctctgcc ccagcaccaa aagtattggc agaatatggc tttactgtag aaaatcttgt
     4981 aaaaattgtt cgaaacttga aataatccta aaaatcaggg cgtaagctct ggtttttctt
     5041 accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa tttgatatag tagtcctatg
     5101 taaaagacaa aggagaaaac aatgaatcca aatattactt ttttaatcat gcttgtaggt
     5161 atgatggcct tgatgttctt tatgcaacgt tctcaaaaga aacaagctca aaagcgtatg
     5221 gaaagcttaa ataaactaca aaaaggctat gaagtgatta caatcggtgg actttatgga
     5281 acagtcgatg aagtagatac ggagaaggga acaatagttc ttgacgtaga tggagtttac
     5341 ttgacttttg aactagctgc tatcaagaca gtattaccgc tgaaagaaac agcttcacta
     5401 gaaggcgcaa ttgaaaaata agacgggatc acgaactccc gtttttctat aaaagaaagg
     5461 aaatgggatg aaaaaattag tctttgtctg tctgggaaat atttgccgta gccctatggc
     5521 cgagtttgtt atgaaatcaa tgacagataa ctacgaaatc caaagtcgag caacttcctc
     5581 ttgggaacat ggcaatccga ttcataaggg gactcaggga atttttcaag agtatgagat
     5641 tccttatgac aagaacaaga catcgcttca gattagtaag gaagattttg aagcctttga
     5701 ttatattatc ggaatggacg cttcaaatat ttctgactta tgtcagatgt gtccagtaga
     5761 ctgtcaagat aagatttact cattttcatc tgaaagtgtt ccagaccctt ggtatacagg
     5821 agattttgaa gaaacctatc gacgtgttca agagggctgt caagcttggt tagaacgttt
     5881 agaaaaggag agttaaggtg gaaaatctta cgaattttta cgaaaagtat cgtgtctatc
     5941 tgactcgtcc acgtttagag cttttggcag tagttaccat tgttttttgt gctgtactcg
     6001 tcttttttct aaatattcca ggaaaaggtg tcttaaaact cgataatgga acgattgttt
     6061 atgatggcag tcttgtccgt ggtaaaatga atggccaagg taccattacc ttccaaaatg
     6121 gagaccaata tacaggtggc ttcaacaatg gagccttcaa cggaaaaggt acctttcaat
     6181 ctaaagaagg ctggacctac gaaggtgatt ttgtaaatgg tcaggctgaa ggaaaaggga
     6241 aactaacaac agaacaagaa gtcgtttatg aaggaacttt taaacaaggc gtttttcaac
     6301 aaaaataaag cctccttatc aaaggaggta ttattagaat cacaaggtaa gcgcttacct
     6361 gtaaatccct ttctttccaa atccctcttc caagcaagtt tgtgaaataa aaaatatttg
     6421 aaataaattt cacaaacttc aaagataaaa cttgataaga aaagaaaatg agaaaagttt
     6481 cacaagagtt taaaaattct ttgtgaaatg tttatgaaac tgtttacaaa gttgaaataa
     6541 tgcgttataa taaacttgtg aaaaaattaa caaaggatat cgttccttga aagctatgga
     6601 ggaaaatatg gctgataaaa aaactgtgac accagaggaa aagaaactcg ttgctgaaaa
     6661 acacgtagat gagttggttc aaaaagctct agttgccctt gaagaaatgc gtaaattgga
     6721 tcaagaacaa gttgactaca tcgttgccaa agcatcagta gcagctttgg atgcccacgg
     6781 agaattggct ttacatgcct ttgaagaaac aggacgtggt gtatttgaag acaaagcaac
     6841 taagaacttg tttgcttgtg aacacgtagt aaacaatatg cgccacacta agacagttgg
     6901 cgttatcgaa gaagacgatg taacaggatt gactcttatt gctgaaccag ttggtgttgt
     6961 ttgtggtatt actccaacaa caaacccaac atcaacagca atcttcaaat cattgatttc
     7021 attgaagaca cgtaacccaa tcgtctttgc cttccatcca tcagcacaag aatcatctgc
     7081 tcatgcagct cgtatcgtcc gcgatgcagc tatcgcagct ggtgctcctg aaaactgtgt
     7141 gcaatggatt actcaaccat ctatggaagc aacaagtgcc cttatgaacc acgaaggtgt
     7201 tgcgacaatc cttgcaacag gtggtaatgc catggttaag gcggcttatt catgtggtaa
     7261 accagctctt ggggtaggtg ccggaaacgt tccagcttat gttgaaaaat cagcaaacat
     7321 tcgtcaagca gcacacgata tcgtcatgtc taaatcattt gataacggta tggtctgtgc
     7381 atctgaacaa gcagttatca ttgataaaga aatttacgat gaatttgtag cagagttcaa
     7441 atcttaccac acttactttg taaacaaaaa agaaaaagct cttcttgaag agttctgctt
     7501 cggcgtcaaa gcaaacagca aaaactgtgc tggtgcaaaa ttgaacgctg acatcgttgg
     7561 taaaccagca acttggattg cagaacaagc aggatttaca gttccagaag gaacaaacat
     7621 tcttgctgca gaatgtaaag aagttggcga aaatgagcca ttgactcgtg aaaaattgtc
     7681 accagttatt gcagttttga aatctgaaag ccgtgaagat ggtattacta aggctcgtca
     7741 aatggttgaa tttaacggtc ttggacactc agcagctatc cacacagctg acgaagaatt
     7801 gactaaagaa tttggtaaag ctgttaaagc tattcgtgtt atctgtaact caccttctac
     7861 ttttggtggt atcggggacg tttacaatgc cttcttgcca tcattgacac ttggatgtgg
     7921 ttcttacgga cgcaactcag ttggggataa cgttagtgcc attaacctct tgaatatcaa
     7981 aaaagtcgga agacggagaa ataacatgca atggatgaaa cttccttcaa aaacatactt
     8041 tgaacgtgat tcaattcaat accttcaaaa atgtcgtgac gttgaacgtg tcatgatcgt
     8101 tactgaccat gccatggtag agcttggttt ccttgatcgt atcatcgaac aactggacct
     8161 tcgtcgcaat aaggttgttt accaaatctt tgcggatgta gaaccggatc cagatatcac
     8221 aactgtaaac cgtggtactg agattatgcg tgccttcaaa ccagatacca tcatcgcact
     8281 cggtggtggg tctccaatgg atgctgccaa agtaatgtgg ctcttctacg agcaaccaga
     8341 agtggacttc cgtgaccttg tccaaaaatt catggatatc cgtaaacgtg ccttcaagtt
     8401 cccattgctt ggtaagaaga ctaaattcat cgcgattcca actacatctg gtacaggatc
     8461 tgaagtaaca ccatttgccg ttatctctga taaagcaaac aaccgtaaat acccaatcgc
     8521 tgactactca ttgacaccaa ctgtggcaat cgtagatcct gctttggtat tgacagttcc
     8581 aggatttgtt gctgctgata ctggtatgga cgtattgact cacgcgacag aagcatacgt
     8641 atcacaaatg gctagtgact acactgatgg tttagcactt caagccatta aattggtctt
     8701 tgaaaatctc gaaagctcag ttaagaatgc agacttccac tcacgtgaga aaatgcataa
     8761 cgcttcaaca atcgctggta tggcctttgc caatgccttc ctaggtattt ctcactcaat
     8821 ggcccataag attggtgcgc aattccacac aatccacggt cgtacaaatg ctatcttgct
     8881 tccatacgtt atccgttaca acggtacacg tccagctaag acagcaacat ggcctaagta
     8941 caactactac cgtgcagatg aaaaatacca agatatcgca cgcatgcttg gacttccagc
     9001 ttctactcca gaagaagggg ttgaatctta cgcaaaagct gtctacgaac tcggtgaacg
     9061 tattgggatc caaatgaatt ttagagacca aggaattgac gaaaaagaat ggaaagaaca
     9121 ttctcgtgaa ttagccttcc tggcttatga agaccaatgt tcaccagcta acccacgtct
     9181 tccaatggta gaccatatgc aagaaatcat cgaagatgca tactatggct acaaagaaag
     9241 accaggacgc cgtaaataat tgtttatcag tctagaagca agacaaaaac tcaatttgag
     9301 ggaaagatcc agtaattttt ctatgataaa aggcatccta tcaaggtttt tgaacacctg
     9361 ataggatgcc tttttatgat attgaggcct ttttgccctt tttgaaaaac tagaatagaa
     9421 acaaaatata taatagattg aaactggaat agtacacttc tgcttctaaa acattgttag
     9481 aa
//
LOCUS       DAVLFB010000083         9026 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLFB010000083 DAVLFB010000000
VERSION     DAVLFB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.083"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..88
                     /locus_tag="VXD58_001684"
     CDS             <1..88
                     /locus_tag="VXD58_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="HEV0676345.1"
                     /translation="KEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            109..1434
                     /locus_tag="VXD58_001685"
     CDS             109..1434
                     /locus_tag="VXD58_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV0676346.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            1431..2183
                     /locus_tag="VXD58_001686"
     CDS             1431..2183
                     /locus_tag="VXD58_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0676347.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            2235..2306
                     /locus_tag="VXD58_001687"
     tRNA            2235..2306
                     /locus_tag="VXD58_001687"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2268..2270,aa:Glu,seq:ttc)
     gene            2312..2385
                     /locus_tag="VXD58_001688"
     tRNA            2312..2385
                     /locus_tag="VXD58_001688"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2346..2348,aa:Asn,seq:gtt)
     gene            complement(2426..2968)
                     /locus_tag="VXD58_001689"
     CDS             complement(2426..2968)
                     /locus_tag="VXD58_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV0676348.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            3147..3914
                     /locus_tag="VXD58_001690"
     CDS             3147..3914
                     /locus_tag="VXD58_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV0676349.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDSYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            3999..4337
                     /locus_tag="VXD58_001691"
     CDS             3999..4337
                     /locus_tag="VXD58_001691"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676350.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            4365..4679
                     /locus_tag="VXD58_001692"
     CDS             4365..4679
                     /locus_tag="VXD58_001692"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV0676351.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(4780..7332)
                     /locus_tag="VXD58_001693"
     CDS             complement(4780..7332)
                     /locus_tag="VXD58_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV0676352.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7394..9016)
                     /locus_tag="VXD58_001694"
     CDS             complement(7394..9016)
                     /locus_tag="VXD58_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676353.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         3014 a         1412 c         1668 g         2932 t
ORIGIN      
        1 gaaggaaaaa gacttacaaa atattaaagg tggggagatg aggatttcaa gaatcatcct
       61 tgattttctt ttcctacgaa aaaagtaatg aaataaggag aaagagtaat ggatttactt
      121 ggatttggga cagttattgt tcatttttta attattagtc acagttaccg tttaatttgt
      181 aaaggtcgaa taaatagaaa agaattatac atttttggtg cttatacatt actaactgaa
      241 atagtacttg aattttcctt ttatcttcta tatttagata aaatagggat tgaaagattt
      301 ttatttcctt tgggcttata ttcctatttt cgatggatga aacagtatga gagggataga
      361 ggactattcc taagtttact actatctctt ttatatgaga gcactcataa ctttctgtcc
      421 gtaactttct cctctataac aggagataat tttgttttac aatatcattt cccattcttt
      481 ttcgttgtga cggtgttaac ctattttgtt acattaaaaa tcatttatta tttccatttg
      541 gaactagcct attttgacaa ggactacctt tatcctttct tgaaaaaagt attttttgct
      601 ttactattgc tacatattgt atctttcgtt tcagatatgg taagtacgat taaacatttg
      661 aatagttttg gaagtatttt gtcatctatt gtctttatct ctctcctttt gaccttcttt
      721 gcaatgaatt ctcataaagt tcaaatggag aaagagattg ctttgaagca gaagaaattt
      781 gaacagaaac atttacagaa ttacacagat gaaattgttg gtctgtataa tgaaatccgt
      841 ggttttcgac atgattatgc tggaatgctt gtcagcatgc agatggcaat tgacagtggt
      901 aatttacagg aaattgacag aatttacaat gaagttttag tcaaagcaaa tcataaattg
      961 cgttcagata agtacactta ctttgatttg aacaacatag aagactcagc tttacgaagt
     1021 ttggttgctc agtcaattgt ctatgctcga aataatggtg tagagtttac actggaagta
     1081 aaagatacga ttaccaagct tccaattgaa ctattggatt tggttcgtat catgagcgtt
     1141 ttattgaata atgctgtcga aggatcggct gatagctata aaaagcagat ggaagtagca
     1201 gttattaaga tggaaactga aacagttatg gtgattcaga attcatgtaa aatgacgatg
     1261 actccttcag gagatctatt tgccttagga ttctccacta agggaagaaa tcgcggagtc
     1321 ggattaaata atgtgaaaga actactagat aagtacaaca atattatttt agaaacagag
     1381 atggaaggca gtacatttag acaaatcatt agatttaaga gggaatttga atgaaagttt
     1441 taattttaga agatgttatt gaacatcaag tgagactaga gagaatattg gatgaaattt
     1501 cgaaagaatc gaatattcca atatcataca agacaacggg aaaagtccgt gaatttgaag
     1561 aatacattga aaatgatgaa gtaaatcagc tttatttcct agatatcgat attcatggaa
     1621 ttgagaaaaa gggatttgaa gtggctcagc tcattcgtca ttacaatcct tacgctatta
     1681 ttgtctttat cactagtcga tcagagtttg cgactctaac ctataaatac caggtatcag
     1741 ccctagattt tgttgataag gatatcaatg atgagatgtt taagaagaga attgagcaaa
     1801 atatcttcta cacgaagagt atgttacttg aaaatgaaga tgttgtagat tatttcgact
     1861 acaattacaa gggaaatgat ttaaaaattc cttaccatga tattttgtat attgaaacaa
     1921 caggggtatc tcataaattg cgcattattg gtaagaattt tgcaaaagag ttttatggta
     1981 ccatgacaga tattcaggaa aaggacaaac atactcagcg attttattct cctcacaagt
     2041 catttttggt aaatataggc aatatcagag aaattgatcg aaaaaactta gaaattgttt
     2101 tctatgaaga ccatcgttgt cctatttcaa gattaaaaat tagaaaatta aaagatattt
     2161 tagagaaaaa atctcaaaag tgattgacaa ttagcaagaa attgatataa tggttatatc
     2221 tgctacagat agaaggtccg ttggtcaagg ggttaagaca ccgccttttc acggcggtaa
     2281 cacgggttcg aatcccgtac ggactatttt atccgccata gctcagttgg tagtagcgca
     2341 tgactgttaa tcatgatgtc gtaggttcga gtcctactgg cggagtatag ataaaaggga
     2401 acacagctgt gttcctcttt ttgtatcaat ttgtatcacc aagcattttc ataaggaagt
     2461 ctgttatttc ttgagaactt tctttttttc catgtgcaat ccaagtttgg cagacaccaa
     2521 aaagtgcatg agttagatag atgctactat attctaattc agtggtattt agattcagtt
     2581 gcataaatcg cttttgtaaa tctatactga gcatgatatg aagtttattt cgtaagaaat
     2641 tttggatttc tttagtccca ttttcagaaa gaagggcagc cagaagtggt tctgactcta
     2701 gatattcaaa gacttctaaa atagcgtctc ttttgtgatg agcatgtttt tgaaaaatat
     2761 attcaaatgt atggaatagc ttgctttgat agtgctcaat cacatcatac ttatccttat
     2821 agtgagtata gaagctggaa cgactaattc cggctttttc tactaatttg acagtagaaa
     2881 ttttatcaaa tggctgttcc atcagtaatt gtaccatagc attttcaata gttcgctttg
     2941 tttttaagcg tttgttactt tcttgcatat ttcctccttg taaacaaatt agactatatg
     3001 tctaaaaata gattttttat cttgtaattt agatttttta atgtataatc tattatatta
     3061 aaattttaga caatatgttt aaaaaaggag aaactaagtt taaagaatgg aaagcaattt
     3121 aaaaaaaacc aacctttatt attgtcatga tcgggatttc tcttattcca gatctgtaca
     3181 atatcatatt tttgtcatca atgtgggatt catatgggca attgtctgac ttacctgtgg
     3241 cagttgtaaa taatgataaa gaggcttcct ataatggtaa tactatggca ataggaaaag
     3301 acatggtgtc caatttaaaa gaaaataaaa ccttggattt tcattttgta gatgaagagg
     3361 aaggaaagaa gggattggaa gatggcgatt actatatggt agtgacttta ccaagtgatt
     3421 tatctgaaaa aacaactaca ttatccaata ttcaatcgac agcagcttat caatcattga
     3481 caagtgagca acaaactgag ataagtgatt ctgtatctca aaattcaact gatagtattc
     3541 aatcggctca gtcaattgta gctttagtac aagatttaca gggaagttta gaaaacttac
     3601 aaaatcaatc ttctaatctt tcgactttaa aaaatcaatc taatcaagta tcacctatta
     3661 cttctacttc tttgatagga ttgtcaagtg gattaacaga gatacaagga gatgttacta
     3721 gcaaattagt tcctgccagt cagtcgattg catcaggtgt aaacgcatat actacaggtg
     3781 ttgataaagt ttctcagggc gcaagtcaac taagtgaaaa aaatgccacc ttgacaggta
     3841 gtttggataa actagtttca ggctcaaaca ccttgacaca aaaatcttct agattgacag
     3901 caggagttgg ttaattacaa tcaggatctg ggcaattagc agacaaatcc agtcagttac
     3961 tttcaggtgc ttctccatta gagaatagag ctaataaatt ggcagatgga tctgggaaac
     4021 tagcagaagg tggaacaaag ttaacttctg gattggaaga tttacagaca ggacttgctt
     4081 ctttaggaca aggactaggt aatgctagtg atcaactcaa atcagtatca acagaatcta
     4141 aaaatgcaga gattttgtca aatccactca atctttcaaa aacagacaat gatcaagttc
     4201 ctgtaaatgg aatcgcaata gctccttata tgatatcagt tgctcttttt gttgcagcaa
     4261 tatcaacaaa tatgatattt gcgaaattgc cttcaggacg tcatccagag agccgttggg
     4321 cttggttgaa atcttgagct gaaataaatg gtattatagc tgttttggca ggaattttgg
     4381 tatatggagg agttcagctt attggtttaa ctgctaatca tgagatgaga atatttattc
     4441 tcatcatcct aacaagttta gtattcatgt ctatggtgac cactttagca acgtggaata
     4501 gccgtatagg agcttttttc tcacttattt tgcttttact acagttagca tcaagtgcag
     4561 gtacttatcc acttgctttg acaaatgatt tctttagatc tattaatccc tggttaccaa
     4621 tgagctattc agtttcggga ttacgacaaa caatctctat caacaagtca ttttcctagc
     4681 tgtcatacta gttctattta ctagtttagg tatgctagcc tatcaacata agaaaatgga
     4741 agaagattaa aaaaatcgac cgattaactg gtcgattttt tatgccttag atgactttcg
     4801 tctgtgatta tagattccaa atagtaagag agaagtaaag gaacagattg ctccagtaat
     4861 aaaaccattg ggaatgaagg aaagtgtaat agttcctttc cccttgggaa tgtcaacttt
     4921 cataaatcca gtttgagctt gtttaatttc tattttctta ccatcttggt aggcagacca
     4981 acctttgtca taaggaatgg tgaagaaaat agatgtatct tgttggacat catatgtagc
     5041 aaaaaccttg tttttagaag ttgatactgt gacaggttgt tctttaattt tttgaattgc
     5101 ctcggtgaaa gttttggtat ctaaacgata gaaggtagga gattcaaatg atacttgtga
     5161 atttccaggg aaactaacat tgatattgaa agtttttttc tctttagtat atcctagatt
     5221 aaagaaggag aagacattat cagttgtaaa agtctttttt tcaccattta caaggatgtc
     5281 aaccttcttt tgtttatcgt tagaaaagtg aaggtttatg aaagagagat aaacttggct
     5341 gttttctgga acttcaattt gatactggat tgctgcatct tcatttgaag aacttgtgac
     5401 actaatcaaa tcattagtat tttctatttt ttctgttttt tcataaggta ttggagaaaa
     5461 ataatcaaaa ttgacgttag caagttgatt taaaaatgag gcctgattat ccaaggtatg
     5521 ttcattgaac ttgacatcat tgtaaacaga ttgactagca actgcaatcg gaagagagta
     5581 ttgattttca tatagggtaa gattatcttt ttgatagata tctttaaagc catacttatc
     5641 aataggactg tctgagatat tgtactggat accaaataaa ctatcagcca aaatactatt
     5701 atttgcatat cggagattga gattagtccc agaggattta aaaccaagtt tatctaaagt
     5761 agagcttgat gaacgatttc gaacagatga aaattgagag attccattgt agttgaattt
     5821 catactgtca tttcctgtct gagtttgtag tttttcagta cgagtaaatt gatttccaat
     5881 atatgttgag aaagattcca tagctgggat atctcgacta taagcacttc gagaagcaaa
     5941 tccccattcc ttagcaattc cgtccatttg agatgaagca tttaaactca tttcaaccag
     6001 tataaataaa gagattagaa tggcaaatag attcacagat ataaactttt tgataactgc
     6061 aaggagtaaa agagaataga caaccaaaaa ttcaagagta agcagaatat tcaaatctgt
     6121 taaaaaagaa taatgcgatt ttagatagat ggtagctaaa aatcctgtta ctacaagaaa
     6181 aagcgaaact aaaaaattcc agactttaag ttctttcaga cgctttaaga cttctgctgc
     6241 tgtgtaaatt aacaaggtag agaaaatcca agcatagcga tgtaaaaaca tgtttggagt
     6301 atgcatgcct tgccaaaata agtcaagagc ttctatgtaa aagcttgcaa ttagaaatgc
     6361 aaagaatatt acatatatga gtttcacgtg aaacttaata gatttcagcg taaaaaataa
     6421 aatggtcaaa ataaagggaa atagtccaac aaaaatcatt gggatggccc catactttgt
     6481 tgtgtcaaag gaaccaatga attgcttagc aaagagatca agataccagc tactttcagt
     6541 ttgaaacttt gtaacttcag tcaatttttc cccatgtgtc tgtaaatcaa atagagtggg
     6601 aagagtcata atcaaactag ccataccagc taaaaaggag ataactatga aatcaagaac
     6661 agatgatttt cgagtcttaa agtcccacga aatttgacag agataccaga aaataagaaa
     6721 caatactgtc atatatccaa aataataatt ttgaataaat aagattgaca gacttgtaaa
     6781 gtacaatagg agtttctttt cagttatcag tagatgtaaa ccagttataa ttaaaggaat
     6841 caagataaaa acatctagcc aggtttttat ctctaattga ctgacagtga aactcatcag
     6901 agcataggaa gtagataagg ctagttttaa agtctgaggg atagattgaa acaatttatt
     6961 caaactaaaa aaggttgaca gaccaatcaa tccaaatttt aagagagtgg tcagatagac
     7021 agcatctggc atattcgtta gatcaaaaaa gtaaactaga ggcgcgagaa aactacccaa
     7081 gtaataacta gatagggcat agaagtttag ccctagacca cttgtaaagg tgtaaaacag
     7141 actactattt ccatgtagga tatttcgtaa ggctacatca aaaataacgt attgatgaaa
     7201 gccatctcct aatagaggag agttgtcgct attccagtag ataccttgag atagatatac
     7261 tccagacata atcactacag gaatgatgaa agaaataaaa taggttcgat atgtttttaa
     7321 aaatgatttc atgttacctc gtagaatgat agaaaactca gttggttaac ccaactgagt
     7381 tttgaagttt tatttagtct ttccaaagtt ctttaacttt tgcttgtact tctgcatttt
     7441 ctaggaattc atcgtaggtt tcatcgatac ggtcaatgac gccattttta gataagacaa
     7501 tgatatggtt agccaaagtt tgaataaact cgtggtcatg gctggcaaag atgattgatt
     7561 ctttaaagtt tttcaatcca tcattcaagc ttgagataga ttccaagtcc aagtgatttg
     7621 ttggatcatc aagtacaagg acatttgatt ttaagagcat gagttttgaa agcatgacac
     7681 gaactttttc tccccctgac aagacattta caggtttgtt aacttcatct ccagagaaga
     7741 gcatacggcc gaggaaacca cgtaggaaag tattgtcatc atcttcttta cttgcgaatt
     7801 gacgcaacca gtcaagaatt gattctcctc ctgcaaaatc agctgagtta tcttttggta
     7861 ggtaagattg actagttgta actccccact tgacagttcc ttcatagtca atatctccca
     7921 tgattgcacg aattaatgca gtcgtttgaa tatcattttg tccaataagt gctgtcttat
     7981 catctggacg caagatgaaa ctaatattat ccaagatagt ttcaccatca atctttacag
     8041 ttagattttc tactgtcaag agatcattac caatctcacg ttccgcttta aagttgataa
     8101 atggatattt acgactagat ggtacaatct cttctagctc aatcttatca agcattttct
     8161 tacgtgatgt tgcctgcctt gacttagaag cattggcaga gaaacgagca acgaattctt
     8221 gcaattgttt aattttttct tctgctttag cattacggtc tgctagcaat ttagcagcaa
     8281 gctcagaaga ttccttccag aagtcgtagt ttccgacata gagtttgatt tttccaaagt
     8341 caaggtcggc catgtgagta caaactttgt ttaagaagtg acggtcgtgg gatactacga
     8401 taactgtgtt atcaaagtca attaagaagt cttctaacca agtaatcgat tggatatcca
     8461 aaccgttagt aggctcgtcc aagagaagaa catctggttt accaaaaagt gctttggcga
     8521 ggagaacctt tactttttca ccgttggcca attcgctcat gttttggtag tgtaattctt
     8581 ctggaatgtt taggttttga agtagttgag aggcttcact ctctgcttcc caacctccaa
     8641 gttcggcaaa ctctccttcg agttcggcag cacgaacccc gtcctcgtct gagaaatctt
     8701 ccttcatgta gatagcatct ttctctttca tgatgctata aagtttttca tttcccatga
     8761 taacgacatc aatggcacgt tcatcttcgt agtcaaagtg attttgacga agaacagaga
     8821 gacgttcatc tggaccaaga gagatgtgac cagtagtagg ttcgatatct ccagctaaaa
     8881 tttttaaaaa ggttgatttt ccggcaccat tagcaccgat taatccgtaa gtatttcctt
     8941 ctgtaaattt gatattgaca tcatcaaaaa gtttgcgatc actaaaacgt agtgaaacat
     9001 cagatactgt aagcaatatt tttctc
//
LOCUS       DAVLFB010000084         8938 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLFB010000084 DAVLFB010000000
VERSION     DAVLFB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.084"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..241
                     /locus_tag="VXD58_001695"
     CDS             <1..241
                     /locus_tag="VXD58_001695"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676354.1"
                     /translation="KVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKL
                     YPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            285..5498
                     /locus_tag="VXD58_001696"
     CDS             285..5498
                     /locus_tag="VXD58_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0676355.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            5752..6603
                     /gene="ylqF"
                     /locus_tag="VXD58_001697"
     CDS             5752..6603
                     /gene="ylqF"
                     /locus_tag="VXD58_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV0676356.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6590..7369
                     /locus_tag="VXD58_001698"
     CDS             6590..7369
                     /locus_tag="VXD58_001698"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV0676357.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7385..8935
                     /locus_tag="VXD58_001699"
     CDS             7385..8935
                     /locus_tag="VXD58_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV0676358.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         2978 a         1531 c         1858 g         2571 t
ORIGIN      
        1 taaagttagt tttatcgatc caaacggttc ttgggctcga caacagaaag tgactattga
       61 taatattaat agacttgaaa aaatgattga agatgcagta aaatttgatg ctgaggatga
      121 agttgccaaa ttatatcctg agacaaatag tagagttctt aaactgaaaa aagcaatctt
      181 taaagcttat cttgaccaaa ccggtgattt tagaagttca atttttgaga ataaaaaata
      241 gtgtttacta ttaggaaata aaaagtgaag gtggaggatt agaattgtca ttattaaaaa
      301 aagataaatt ttccattcgg aaaataaagg gtattgttgg ttcggtattc ttggggagtc
      361 ttttatttgc accgtcagtt gttggtgcat cgacttatca ttacttagat tatagtagtt
      421 tgacacaaac tgaacgtgat caactcaaac aaggtagacc tgatgaatcc aaagagtcat
      481 atgctttgga ttatgaaaaa gacgcactac caaacacagg aagttctcaa tctattatga
      541 ctgcattggg tttgttagct atcgggagtc ttattgtgat tatcacaaaa gataatagaa
      601 ataaaaaaat agctacattt ttgattgtag gggcgacagg tttagttaca ctatcaactg
      661 cttcagcact taatttgaat gctaacatcc atgagtctgg acgtgatggt gtgttgcaaa
      721 tttctggtta cagatatgtt ggttacttgg agcttgatga taaaactgtt tcgtcggttt
      781 ctccagcatc aactgtttcg ccagttgagc aacccaaagt tgtaacagag aaaggcgaac
      841 cggaagttca tgagaagcct gactatacgc aaccaatagg tgcaaacctt gttgagcctg
      901 aagttcatga gaaactagca tacactgaac cagtagggac aacaggagta gatgaaaatg
      961 gaaacttgat tgaaccacct gttaacgaca tcccagaata tactgagcca gtaggtacaa
     1021 caggagtgga tgaaaatgga aacttgattg agccacctgt tagcgacatt ccagagtata
     1081 ctgaaccaat atctacagtt tcagaagttg catcagaaag agaagagttg ccttctttac
     1141 ataccgatat tcggacagag actattccta aaaccactat agaagaatct gatcctagca
     1201 aatttatagg cgatgattct gttagacaag ttggtgagga tggcgaacgt caaattgtta
     1261 ctagctatga agagttacat ggcaaaaaaa tcagtgaccc agttgaaaca gtaactattt
     1321 tgaaagaaat gaagcctaaa attcttgtaa aaggtaccaa agaaaaacct aaagaaaaaa
     1381 cggctcctgt tttgacctta gatagaacaa atacaaatgt attaaaccga tccgcaactc
     1441 tttcttatca tttagttaat acagatggag tcacaatcaa taaaattact gcgacaatta
     1501 aagatggaaa tgaaattgtc aaaacagtag atttgacttc agagcaatta gataagcagg
     1561 tagaagattt aaaattctac aaggactata agatagagac cactatgacc tatgaccgtg
     1621 gcaagggaga agaaactgca acattagaag agaaaccact tcgcttggat ttgaaaaaag
     1681 ttgaaattaa aaatatcgca tctactaatt tagtgaaggt aaatgacgat ggtactgaaa
     1741 cacctagtga ttttatgact gaaaaacctt cagatgaaga tgtgaagaaa atgtacttaa
     1801 aaattactag tcgtgataat aaagtaacac gtttagcagt tgataaaatt gaagaagtga
     1861 ctgaagaagg taagaaactt tataaaatca ctgccgaagc tcaagatttg attcaacata
     1921 cagacccaac gaaggttcgt aacaagtatg ttcattatat tgaaaaacca gttccaaaag
     1981 ttgatgacgt ttattataac tttaaagagt tagtagatgc catgaatgct gataaaaatg
     2041 gtacatttaa aatcggagca gatttgaatg caacgaatgt accaacacct aacaaacaat
     2101 atgttccagg tacgtttaag ggacatctat caagtgttga tggtaaacaa tatacaattc
     2161 ataacattgc ccgtccctta tttgaccgtg ttgaaaatgg ttctgtgaaa aatattaatt
     2221 taggtaatgt ggatatcaat atgccttggg ctgacggaat agcacctgtt gccaatatgg
     2281 tgaaaaatgc aactgttgaa gatgttaagg taactggtaa tgtagttgct aataacaata
     2341 ttgcgggtat cgtcaataaa atcgatagtg gtggtcagtt gacaaatgta gccttcatcg
     2401 gtaatcttac aggtgttggt gataaaggtc aatatatggc tggcattgcc ggtgagattt
     2461 ggagaggtaa tcttgcaaaa gcatatgtcg aggctgatat tgttgctaac agagcccgta
     2521 taggtggttt agttgctaaa acagataatg gtaacgactc tatgggaatt ggtaaatacg
     2581 gttcaattcg taaatcagtc accaaaggaa ccattaagac taaagttttg tttgaaacag
     2641 gtggatttat caatagcaat cttccttttg gtaaacttga agataatatt tcaatgatga
     2701 gagttgaaaa tggtgaagag ttctttggtt cttccgattt ggactacgat ggtggttatt
     2761 tcacaaatgg ctggttagag cgtaactttg ttgtcaaagg tgtcagttca ggtaagcatt
     2821 catacaaacg ttctcgtgac aaaattaaag aaattagtca agatgaagct aataagagaa
     2881 ttgcaaattt tggacttact gctgataaat acgaaatcaa cgaacctgtg gtcaatcgct
     2941 taaatcgttt aaccagaaga gaagatgaat ataagtcaac tcaagactac aaatccgaac
     3001 gtgatttagc ttatcgtaat attgagaaac ttcaaccatt ttataataaa gaatggattg
     3061 tcaaccaagg taacaaactt gcagaagatt caaacttagc taagaaagaa gtcctttctg
     3121 ttacggggat gaaggatggt caattcgtga ccgatttatc agatattgat aagattatgg
     3181 ttcactatgc tgatggtact aaggaagaaa tggatgttac taagaatact gattctaaag
     3241 tccaacaagt gcgtgaatat agtgtatctg gtcttggtga tgttgtttat acaccgaata
     3301 tggttgttaa aaatcgtgat aaattgattg ctgatgttaa atctcaatta tcaagtgtgg
     3361 aattgatttc acaagaagta agagatttaa tgagtagacg tgataaacct gcagagaata
     3421 cggatgaacg taagaatggt tatattaaag atctatactt agaagaaagc tttgccgaag
     3481 tgaagcaaaa tctagataag ttagtcaaat ccttggtcga aaatgaagac catcaattga
     3541 atggtgatga agctgcgata aaatcacttc tcaagaaagt ggaaaccaat aaagctaaaa
     3601 tcatgatggc tctgacttat ctcaatcgtt actatgatat caaatacggt gacataagta
     3661 taaaaaacat catgatgttc aaacctgatt tttatggtaa aacaccaagt gttattgaca
     3721 gactcatcaa catcggttca agtgaaaaga acttgaaagg ggatcggact caggatgctt
     3781 accgtgaaat catcgcaggt aatactggta aaagtaattt gcgtaacttc ttagaataca
     3841 acatgcgtct gtttacagag gataaagaca tcaatgattg gtttatccac tcagctaaga
     3901 atgtttatgt ttcagaacct aagacaacaa atacagagtt gaaagataaa cgtcatcgtg
     3961 tgttcgatgg tttagataac ggtgttcatg gtcgtatgat tttaccactt ctaacgctta
     4021 aagatgccca tatgttctta atttcaacat ataacacgat ggcgtacagt tcatttgaga
     4081 aatatggaaa acatacagaa gaagctcgta atgagttcaa aactaagatt gatgaagttg
     4141 cacatgctca acaaacttat ttagatttct ggtccagatt agctctgcct aatgttcgtg
     4201 atagacttct taagagtcaa aatatggtac cgacaccagt ttgggataac cagacctaca
     4261 atggttctcc tgtaggtcgt cgaggatttg acagtaaagg taaccctata gctccaattc
     4321 gtgaattata tggtccaaca tggcgacacc atgatagaga ctggcgtatg ggcgctatgg
     4381 catcaatatt ccctaatcca aataatgatg acaaggtctt gtttatggtg acggatatga
     4441 ttagcccatt tggtatttca gcctttactc acgaaacaac acacgtaaac gatagaatgc
     4501 tttacttcgg tggtcataag catcgtcaag gtacagacgt tgaagcctat gctcaaggta
     4561 tgttgcaaac acctgacagc tcaacgacta atggtgaata cggcgcactt ggaattaaca
     4621 tggcttacca tcgtccaaat gatggtaacc aatggtacaa cccagatcct gacaaattaa
     4681 agacacgtga tgacattgac cgctacatga gaaactacaa tgaagctatg atgttgcttg
     4741 atcatgtaga agctgatgct gtacttccta aaattaaagg agacaatagt aagtggttca
     4801 agaagattga caaagaaatg cgctctaaga ttcagtataa tgatctttta ggtccaaacc
     4861 aatgggatag tatccgtgat ttgaaagatg aagaaaaggt aatgacttta tctagcgtca
     4921 atgatttagt ggataataac ttcatgacaa aacatggaaa tccaggtaat ggacgttata
     4981 gaccagaaga ttttacacca aactcagctt acgtaaatgt caatatgatg gcaggaattt
     5041 acggtgggaa tacgagtcaa ggtgctcctg gttcattatc atttaagcac aatgctttcc
     5101 gtatgtgggg ctattatggt tatgaaaatg gctttattag ttatgtatca aataaataca
     5161 aagcagaagc agataaaaac aaccacggtt tactaagcga taagctaata attaataaag
     5221 tatcgaaagg aaactttaat actcttgaag agtggaagag acattggtac ggggaagttc
     5281 ttgctaaagc taagaaaggt tttgaggcta tcgatattga tggcgtccat atcagtaact
     5341 atgatgagtt aaggccttta tttgataaag cagttgaaga agatttgaag aaaccagatg
     5401 acttctctca cactgttgct cttaaatcta aggtcttcaa agcccttctt aaaaacacag
     5461 acggtttctt taataaactg tttaaagaag acatttaaat gtttagaata tagaaggaac
     5521 agttttttgc tccctctgaa aagtcatcat ttgatggctt ttttctatat agggtacttg
     5581 aaaggctatc atcggacaaa ataaagaagg catgatataa tataaggtag atttctatgc
     5641 cataaaacaa aaaactgttt gaacatcatt catttgagaa ccctttatgt tcaaacgata
     5701 gtaaaataaa ataggatctc taaacccttg ctacgaaagg aaaaaaactc aatggctact
     5761 attcaatggt ttcctggtca catgtctaaa gcgcgtcgac aggtgcagga aaatttaaaa
     5821 tttgttgatt ttgtaacgat tttggtggat gcacgcttac ctctatctag tcaaaatcct
     5881 atgttgacca agattgttgg tgataaacca aaactcttga ttttaaataa ggcggatttg
     5941 gctgatccag caatgaccaa ggaatggcgt cagtattttg aatcacaagg aatccaaact
     6001 ctggctatca actccaagga gcaagtgact gtaaaagttg taacagatgc ggccaagaag
     6061 ctcatggctg ataagattgc tcgccagaaa gaacgtggga ttcagattga aaccttgcgt
     6121 actatgatta tcgggattcc aaacgctggt aaatcaactc tgatgaaccg tttggctggt
     6181 aaaaagattg ctgttgttgg aaacaagcca ggggtcacaa aaggtcaaca atggcttaaa
     6241 accaataaag acctggaaat cttggataca ccggggattc tctggcctaa gtttgaggat
     6301 gaaactgttg cacttaagtt ggcattgact ggagctatca aggatcagtt gcttcctatg
     6361 gatgaggtta ccatttttgg tatcaattat ttcaaagaac attatccaga aaagctggct
     6421 gaacgcttca aacaaatgaa aattgaagaa gaagcgcctg tgattattat ggatatgacc
     6481 cgcgccctcg gtttccgtga tgactatgac cgtttttaca gtctcttcgt gaaggaagtc
     6541 cgtgatggca aactcggtaa ctatacctta gatacattgg aagacctcga tggcaacgat
     6601 taaagaaatc aaagaattcc ttgtgacagt caaggagtta gaaagcccta tttttttaga
     6661 gcttgaaaag gataatcgct caggagttca aaaggaaatc agcaagcgta aaagagccat
     6721 tcaagctgaa ttagatgaaa atttgcgctt ggaatccatg ctttcttatg aaaaagaact
     6781 ttataagcaa ggattgacct taattgtagg tattgatgag gttggtcgtg gtcctcttgc
     6841 tggtcctgta gtcgctgcgg ccgtcatttt acctaaaaat tgtaagatta aaggtctcaa
     6901 cgacagcaag aaaattccta aaaagaaaca tctggagatt ttccaagccg ttcaagacca
     6961 agccttgtcg attggaattg gtatcataga taatcaggtc atcgaccaag tcaacatcta
     7021 tgaagcaacc aaactagcca tgcaagaagc aatctcccag ctcagccctc aaccagagca
     7081 ccttttgatt gatgccatga aactggactt gcccatttca caaacctcca ttatcaaagg
     7141 agatgccaac tccctctcta tcgcagcagc atctatagta gccaaggtaa cacgtgatga
     7201 attgatgaag gaatacgatc agcagttccc tggctatgat ttcgctacta atgcaggata
     7261 tggcacagct aaacatctgg aaggcctcac aaaactagga gttaccccaa ttcaccgaac
     7321 cagctttgaa cccgttaaat cactggtttt aggtaaaaaa gaaagttaat tgaaaggaaa
     7381 taccatggag gaacagtcgg aaatagtccg ttctaagaaa gaattcgcct ttgcatccag
     7441 cactatacta tcccaagttg gtcgaggaat cattgtcggc ctcatcgttg gaattatcgt
     7501 cggatccttt cgtttcttaa ttgaaaaggg cttccacctg atacaaggag tttatcaaga
     7561 tcaagggtac ttagtgcgca atctttttgt actggttttg ttttatatac tcatctgttg
     7621 gctcagtgcc aaactaacac ggtcagaaaa agatattaaa ggctcaggaa ttcctcaagt
     7681 cgaagccgaa ctgaaaggcc tcatgtccct caactggtgg ggcattcttt ggaaaaaata
     7741 tgtgctaggt attcttgcta ttgccagtgg actcatgctg ggtcgagagg gacccagtat
     7801 tcaacttgga gcagttggtg gtaaaggaat tgccaagtgg ctcaaatcca gtccagtaga
     7861 ggaacgttcc ttgattgcca gtggagctgc agcaggttta gccgcagcct ttaatgctcc
     7921 tattgcagca cttctctttg ttgtagaaga agtctatcac catttttcgc gctttttctg
     7981 ggtctcaact ctagcagcca gcatcgtagc aaactttgtg tctctactca tgttcggttt
     8041 gacaccagta ttggatatgc cagataacat tcctcccatg accctagatc agtattggat
     8101 atatctcgtc atgggaattt tccttggatt ttcaggtttt ctctatgaga aagctgtatt
     8161 aaacgttgga agagtttatg acttgattgg tcaaaaaatc catttggata gggcttatta
     8221 tcccatcttg gcttttatcc ttatcatacc agtcggaatc ttcttacctc aaatcattgg
     8281 tggcggaaat cagcttgtcc tttctttaac tgaacaaaat tttagtttcc aagttttatt
     8341 agcttacttt ttaatccgct ttatttggag tatgattagc tatggaagtg gactgccagg
     8401 aggaattttc ctccccattt tagctcttgg ttctttgctt ggtgccttag ttggtgttat
     8461 ctgtgtcaat cttggacttg tcagtcaaga gcaattccct atatttgtca ttctaggaat
     8521 gagtggctat tttggagcca tatcaaaagc tcccttaacc gctatgatcc tcgtaactga
     8581 gatggtagga gatattcgca accttatgcc acttggtctt gtcactcttg tttcttatat
     8641 tatcatggat ttgctcaaag gtacgccagt ctatgaagcc atgctggaaa aaatgcttcc
     8701 agaagaagta tctagcgaag gagaagttac acttatcgaa ataccagttt ctgataaaat
     8761 tgctgggaaa caagttcatg aactcaactt accacacaac gtcctcatca caactcaagt
     8821 ccataatggc aagagccaaa cagttaacgg ctcaaccaga atgtatctgg gtgatatgat
     8881 tcacctggtt attccaaaaa gtgaaattgg aaaagtcaaa gatttgttgt tgtagtat
//
LOCUS       DAVLFB010000085         8868 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLFB010000085 DAVLFB010000000
VERSION     DAVLFB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.085"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            25..180
                     /locus_tag="VXD58_001700"
                     /pseudo
     CDS             25..180
                     /locus_tag="VXD58_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            380..3655
                     /gene="rexB"
                     /locus_tag="VXD58_001701"
     CDS             380..3655
                     /gene="rexB"
                     /locus_tag="VXD58_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV0676359.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3652..7302
                     /gene="addA"
                     /locus_tag="VXD58_001702"
     CDS             3652..7302
                     /gene="addA"
                     /locus_tag="VXD58_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV0676360.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLPDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKANFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7562..8740
                     /locus_tag="VXD58_001703"
     CDS             7562..8740
                     /locus_tag="VXD58_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676361.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2735 a         1865 c         1887 g         2381 t
ORIGIN      
        1 ctatagttca atcattctat caagaatatt gaatatataa aggcacgtaa tattaaaact
       61 tcttctgata tcgaattaga gtccgatgtt gatggcgata aatcagacaa tctaccagtc
      121 gacattaaga tactgggaca acacattgaa gtgtattccg tacctaagaa acaatcataa
      181 aactcaaagc cttgttaccg aggctttttt attatgtgat tttcagtagc cagacttatt
      241 ctatctaata caagtaaagc taagtattta agatttagca gatgatgaag aaaagaaagg
      301 atggcctaaa tattttaaca gatttacgag gctaatcatg gtataatata gggtgatgaa
      361 aaattccaac gaggctgaga tgaaattact ttatactgat attcggactt ctttgacaga
      421 aattctaaca agagaggcag aagagctagt tgcagctggc aagcgggtct tctacattgc
      481 ccccaactct ctttcttttg aaaaggaacg cgccgtgctg gaatgcttgt cccagcaggc
      541 ttctttttcg attaccgtca cgcgctttgc tcaaatggct cgttatctgg tcttgaatga
      601 tttaccagct aaaactactc ttgatgatat cggtcttggg ttggcctttt acaaatgcct
      661 tgccgaactc gatcccaagg acttgcgtgt ttatggcgct attaagcagg atcctcaatt
      721 gatccagcag ttaattgagc tttatcatga gatgaccaaa tcccagatga gttttttgga
      781 cttggagaat ttaacagatg aggataagag ggcggattta ctcttgattt ttgagaaagt
      841 aacagcctat cttaatcaag gtcagttagc ccagggaagt cagttgtccc atttgattga
      901 ggctattgag aatgacaagg taagtagtga ttttaatcaa atcaccttgg tcattgacgg
      961 ctttactcgt ttttctgctg aggaagagcg ggttgtggac ttacttcacg gcaaaggtgt
     1021 tgagattgtt atcggggctt atgctagtaa gaaagcctat accagtcctt ttagcgaggg
     1081 caatctctac caagccagcg taaaatttct ccatcatctg gcttctaaat accaaacgcc
     1141 tgctcaggac tgttctcaaa ctcatgagaa gatggatagt tttgacaagg cctctcgttt
     1201 gttggagtct tcttatgact tttcagaact cgctttggat gtcgatgaga aagaccgtga
     1261 aaatttacaa atctggtctt gtttgacgca aaaggaggag ttggagctag tagcccgtag
     1321 tattcgtcag aaattacatg agaactcaga cctgagctac aagcattttc gtattctctt
     1381 gggggatgta gcttcttacc agttatctct caaaaccatt tttgaccagt atcagattcc
     1441 tttttatctt ggtagaagcg aagccatggc tcatcatccc ttgactcagt ttgtcgagtc
     1501 tattttagct ttaaaacgtt accgttttcg tcaggaggat ttgattaatc ttcttagaac
     1561 tgatttgtat actgacctca gtcagtctga tattgatgct tttgagcaat atatccgcta
     1621 tcttggtatc aatggcttgc cagcctttca gcaaatcttc accaaatccc accatggaaa
     1681 atttaatctt gagcgtttga atgtcctccg cctgagaatt ttagcacctc ttgaaaccct
     1741 ctttgccagc cgaaaacaaa aggctgaaaa cctcctacaa aaatggagtg tctttctaaa
     1801 agaaggagct gtgaccaagc agttacaaga tttgacaacc actttggaag ctgtagaaca
     1861 ggaaagacaa gccgaagttt ggaaggcttt ctgccatgtt ttagaacaat ttgcgactgt
     1921 ttttgctggt tcacaggtta gtctggaaga cttcctagcc ttgctccatt ctggaatgag
     1981 tttgtcccaa taccgtacca ttccagcaac agtggacact gttctggtgc agagttacga
     2041 tttgattgca ccattgactg ctgactttgt ctatgctatt ggactaactc aggacaattt
     2101 accaaaaatt tctcaaaaca ccagtcttct gacagatgaa gaaaggcaaa acctaaacca
     2161 agcgaccgaa gaaggcgttc aattactgat tgccagcagt gaaaatctca agaaaaatcg
     2221 ctacactatg ctttccttgg tcaattctgc tcgtaagcag ttgttcttgt cggctccaag
     2281 cctttttaac gaaagtgaaa gtaaggaatc tgcctatctt caagagttga tccattttgg
     2341 atttaggcgg agagagaaga ggatgaatca caaaggactg tctaaggagg atatggggtc
     2401 ctatcacagt cttttgtcta gtctggttgc ctatcaccag cagggtgaga tgagcgatac
     2461 tgagcaagat ttgacttttg tcaaggttct gtcgcgtgtc ataggtaaaa aactagatct
     2521 gcaaggtctg gaaaatccag ctatcccaac cagtccaagc agcaagacct tagccaagga
     2581 caccttgcaa gctctctatc ctgccaaaca ggagttttac ctgtctacgt cgggtttgac
     2641 agagttttat cgcaatgaat acagttattt cctacgctac gttttaggct tgcaggagga
     2701 attacgtttg cgtcctgatg cccgtagtca cgggaatttc ttgcatcgta tctttgaacg
     2761 cgccttacag ttgcctaatg aagattcctt tgaccaacgt ctagaacaag ctattcaaga
     2821 aaccagtcaa gaacgcgaat ttgaagctat ttatcaagaa agtttggaag cccagtttac
     2881 caaggaagtt ttgcttgatg ttgcacggac gactggacat attctccgac acaatccagc
     2941 catcgaaacc atcaaagaag aagcaaattt tggtggaaaa gaccaagcct ttattcaatt
     3001 agacaatgga cgcagtgtct ttgtacgagg caaggtggac cggattgacc gtttgaaagc
     3061 taatggagcg ataggagtag tagactacaa atccagtctg actcagttcc agtttcctca
     3121 tttctttaat gggctcaatt ctcagttacc aacctatctt gctgccctaa aaagagaagg
     3181 gaagcagaac tttttcggcg ccatgtactt ggaaatggct gaacctgtcc aatctctgat
     3241 ggcggtaaaa agtctggcag gagcagtggt agaagccagc aaatctatga aataccaagg
     3301 gctcttcttg gaaaaagaaa gcagttattt aggcgaattt tataacaaaa acaaggctaa
     3361 tcaactgaca gatgaggaat ttcagctcct actggactac aatgcctatc tttacaagaa
     3421 agctgctgag aagattttag caggccggtt cgccatcaat ccttatactg aaaatggcag
     3481 aagcattgcc ccatacgtcc agcaacatca ggctattaca ggctttgaag ccaattacca
     3541 tctgggccaa gcccgtttcc tagaaaaatt ggacctagct gatggcaagc gtctggtcgg
     3601 agaaaaactc aagcaagctt ggtttgaaaa aataagagag gagttaaatc gatgaagctt
     3661 attccctttt taagtgagga ggagattcaa aaactgcaag aagcagaagc aaattcgagc
     3721 aaggaacaga agaaaactgc cgaacaaatc gaagctatct acacttctgg tcagaatatc
     3781 ctggtctcag catcggctgg ttctggaaag acctttgtca tggcagagcg cattctggac
     3841 caattggcgc gtggtgtcga aatttctcaa ctctttatct caacctttac cgtcaaggct
     3901 gcaactgaac ttaaagaacg tttagagaaa aaaatcagca agaaaatcca agaaacagat
     3961 gatgtcgatc tcaaacaaca cttgggtcgc cagttggcag acctacccaa cgctgccatc
     4021 ggaaccatgg actctttcac acaaaaattc cttggcaaac atggttatct gcctgatatt
     4081 gcacctaatt tccgtatttt acaaaaccaa agcgagcaac ttcttctcga aaacgaagtc
     4141 tttcatgagg tctttgaagc gcattaccaa ggtaaacaga aagagagctt tagtcatttg
     4201 ctgaaaaact ttgctgggcg tggcaaggac gaacggggtc tgcggcagca agtctataaa
     4261 atctatgact tcctccaatc caccagtaat cctcaaaagt ggctgagtga atctttcctc
     4321 aaaggctttg agaaagctga ttttaccagt gaaaaagaaa aactgactga gcaaatcaaa
     4381 caagccctct gggatttgga aagctttttc cgttaccatc tggataacga tgccaaggag
     4441 tttgcaaagg ctgcctattt agaaaatgtt cagttaattc tggatgaaat tggctcccta
     4501 aatcaggagt tcgatagtca ggcttatcag gcagtacttg cgcgtgttgt cgccatctct
     4561 aaggagaaaa acggtcgtgc tctgactaat gccagccgta aggctgattt gaagcccctg
     4621 gctgatgcct acaacgaaga gagaaagacc cagtttacta aactaggaca attatcagac
     4681 cagatagcga ttctcgacta tcaagaacgt tatcatggag acacttggaa actagctaaa
     4741 accttccaat ctttcatgag cgattttgta gaggcttatc gtcagagaaa acgacaggaa
     4801 aatgcctttg aattcgctga tatcagccat tacaccattg agattttaga gaatttccca
     4861 caagttcgtg agtcttatca ggagcgcttc catgaagtca tggtcgatga gtatcaggat
     4921 accaaccata ttcaagaacg gatgctggaa ttgttgtcta atggccacaa tcgctttatg
     4981 gtgggagata tcaagcaatc catctatcgt ttccgtcagg cagacccgca gattttcaat
     5041 gagaaattcc aacgctatgc gcaaaatccc caagaaggca agctcattct cctcaaggca
     5101 aatttccgta gtagttcaga agtgctgtca gcaaccaatg atgtctttga acgtctcatg
     5161 gaccaagagg tcggcgaaat caactatgat aacaagcacc agcttgtttt tgccaatacc
     5221 aaactgactc ccaatccaga caacaaggca gaatttctcc tctacgacaa ggacgataca
     5281 ggtgaggaag aagagagtca aacagaaacg aaactaacag gcgaaatgcg cttagttatc
     5341 aaggagattc tgaaacttca tcaagaaaaa ggtgttgcct ttaaggaaat tgcccttctg
     5401 acctccagcc gcagtcgtaa tgaccagatt ctcctcgccc tgtctgagta cggaattcct
     5461 gtcaaaactg acggagagca aaacaattat ctccaatccc tagaagtgca agtcatgcta
     5521 gacactcttc gtgtcattca caatcccctg caagactacg ccttggttgc ccttatgaag
     5581 tctccaatgt ttggttttga tgaggatgag ctagcacgtt tgtcccttca gaaagcagag
     5641 gataaagtcc acgaaaatct ctatgagaaa ctggtcaatg cacaaaaaat ggcaagtagt
     5701 caaaaaggct tgattcacac agctctagct gaaaaactaa agcaattcat ggatatccta
     5761 gcttcttggc gcttgtatgc caaaacccac tctctctatg acttgatttg gaagatttac
     5821 aacgaccgtt tttattatga ctatgttggg gctttgccga atggtcctgc taggcaggcc
     5881 aatctctatg ccctagcact gcgtgctgat caatttgaaa agagcaattt caaaggtttg
     5941 tcgcgtttta ttcgtatgat tgaccaagtc ttagaagccc agcacgattt ggcaagcgtg
     6001 gccgtcgcac cgccaaaaga tgcagtagag ctcatgacca tccacaagag taaagggctg
     6061 gagtttcctt acgtctttat cctcaatatg gatcaagatt tcaacaagca agactctatg
     6121 tcagaagtca ttctcagtcg tcagaatggt cttggtgtca aatatattgc caagatggag
     6181 acaggggcag tagaagacca ctatcctaaa accatcaaac tctccattcc tagtctgacc
     6241 tataggcaga acgaagagga attacagcta gcaagctatt ctgagcagat gcgtttgctg
     6301 tatgttgcta tgacgcgggc tgagaaaaag ctctatcttg tcggcaaggg ttctcgtgaa
     6361 aagctggaag tcaagcaata cccagcagcc aaaaatggga aactaaatag caatactaga
     6421 ctgcaagcac ggaatttcca agattggctt tgggctatca gtaaagtgtt tactaaggac
     6481 aagctcaact ttagttatcg ttttattggc gaagatcagt tgaccagaga agctatcgga
     6541 gagttggaaa ccaagagtcc tctccaagat agctcccaag cagacaatcg tcagtcagat
     6601 accatcaaag aagctctgga aatgctgaag gaggtggaag tttataatac tcttcaccgc
     6661 gcagctattg aacttcctag tgttcaaacc ccaagtcaaa tcaagaaatt ctacgaacca
     6721 gttatggata tggaaggtgt cgagattgct ggtcaaggtc agtcagtagg caagaaaatc
     6781 agcttcgatt tgccagattt ttcaaccaaa gaaaaggtaa ctggagctga gattggtagt
     6841 gctactcacg aactcatgca gagaattgac ctcagccagc aactaaccct tgctagccta
     6901 acagaaacac tcaaacaagt tcaaactagc caagctgtca gagacaagat caatcttgat
     6961 aaaattcttg ctttctttga cacagtactc ggtcaggaaa ttcttgctaa taccgaccat
     7021 ctctatcgcg agcaaccttt ctccatgctc aaacgagacc aaaagagtca ggaagacttt
     7081 gttgtccgtg gtatccttga tggctatctg ctttacgaaa acaaaattgt tctgttcgac
     7141 tacaagacag accgctatga tgaaccaagt caactcgtag accgctatcg tggtcagtta
     7201 gctctatacg aagaggcttt atcacgagcc tattcgattg aaaatattga aaaatacttg
     7261 attttactcg gtaaagacga ggttcaagtt gtaaaagtat aacttatact ctttgaaaat
     7321 ctcttcaaac cacgtcagct tcgccttgct gtatatatgg ttactgactt cgtcagttct
     7381 atccacaacc tcaaaacagt gttttgagct gacttcgtca gttctatcta caacctcaaa
     7441 acagtgtttt gagctgactt cgtcagttct atctacaacc tcaaaacagt gttttgagct
     7501 gattgcggct agctttctag tttgctcttt gatttttatt gagtattaga aaggagacct
     7561 catgacactt ccagttagaa aatccctgca cgatgcagtt ttacaggctt cgaaagcgga
     7621 tacttgggaa caagctacca aggaatggaa tgaagtttcc ttgattttta acggtatcgg
     7681 ccgtagcaat tgtgtctgtg gaaatgccat caaatacgct tatgaactct ttaacggtgt
     7741 cacaggccaa cgcctctttc caattggtag cgactgtgtt cgtcattttc atcgattgtc
     7801 ccttgaccag caattggaag aggaagaaaa actgctcaga aaggttgaaa atctaaccag
     7861 aaaagctcag aaaaaggaaa aaatcaaggt caataaaagt gactttgacg agcgacttct
     7921 aaaatggctc tgggaaaaag gtgttttcaa acccaatcgt ggcaatcaat ttgcgcctga
     7981 gagagactac cagcttttcc tagaagtctt tcagggagga agttggacca aggcggaacc
     8041 aaagaaaaag gctcggatgg aagaagtcct tgaaaagtgt atcaaaccct ttttacttgg
     8101 gaaatcggat gaccaactct accttgtcaa gctaggcaag gagaaaattg actacgaaca
     8161 agaactccgt atccaggcag agaaagaacg caagaagagg gataaaatcg ccaagcaata
     8221 tgcagacaat ctggttctag ccatgggacc cgcagagcgt gcttatcaag attactttgg
     8281 ctttacagag accctgactc aagaagaacg caagtgggaa aaaattcttt ttggtaaaaa
     8341 tagagcagaa cgggctatca aggccaaaca ataccaaaaa gagctggaaa aagaccaacg
     8401 aattgccagt caggatccaa ttgaaagaaa acagaagcag acttggctcc tcaattccta
     8461 ttttcgtgag cttcctgaag aaaaagctcg attttctagg ctcttattag aatatcgaaa
     8521 aagtggtgaa gtaccctttt caactgaata cctgtccgat catctcatcg actttttcta
     8581 taaaatgaaa gcctttgaat ttgaaatcgc accagaacaa gtccgagatt ttctaaaaaa
     8641 aagcctgcag gaagaccatc gctcatcagc acaaggaagc tggatagaag gaattctcct
     8701 caactgcctc aaaccatttt tagaacgatt agtgatatga gtaacaccta ccgtgtaaat
     8761 ttcatggtag gtgttattgt acccccaaat aatataaaac aaagtatgaa agcgcatctc
     8821 aattttaaga cttttttgta aattaagagt ataattaagg tatactgc
//
LOCUS       DAVLFB010000086         8753 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLFB010000086 DAVLFB010000000
VERSION     DAVLFB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.086"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            27..2381
                     /gene="rnr"
                     /locus_tag="VXD58_001704"
     CDS             27..2381
                     /gene="rnr"
                     /locus_tag="VXD58_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV0676362.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNH
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYVDVLSTMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2344..2811
                     /gene="smpB"
                     /locus_tag="VXD58_001705"
     CDS             2344..2811
                     /gene="smpB"
                     /locus_tag="VXD58_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV0676363.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            2827..3687
                     /gene="tehB"
                     /locus_tag="VXD58_001706"
     CDS             2827..3687
                     /gene="tehB"
                     /locus_tag="VXD58_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV0676364.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            3760..4713
                     /gene="coiA"
                     /locus_tag="VXD58_001707"
     CDS             3760..4713
                     /gene="coiA"
                     /locus_tag="VXD58_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV0676365.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            4732..6534
                     /gene="pepF"
                     /locus_tag="VXD58_001708"
     CDS             4732..6534
                     /gene="pepF"
                     /locus_tag="VXD58_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV0676366.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6536..7249
                     /locus_tag="VXD58_001709"
     CDS             6536..7249
                     /locus_tag="VXD58_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV0676367.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7316..8257
                     /gene="prsA"
                     /locus_tag="VXD58_001710"
     CDS             7316..8257
                     /gene="prsA"
                     /locus_tag="VXD58_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV0676368.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8374..8715)
                     /locus_tag="VXD58_001711"
                     /pseudo
     CDS             complement(8374..8715)
                     /locus_tag="VXD58_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2821 a         1632 c         1914 g         2386 t
ORIGIN      
        1 aaaattaaaa aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca
       61 agggaaaggt gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt
      121 ttcgtgagtt gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag
      181 aagatggtag tctgacatta gaaattaaga aaaaacatga gattaccctc aaggggattt
      241 ttcatgccca taaaaatggc tttggctttg ttagtctgga aggcgaggag gacgaccttt
      301 ttgtagggaa aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta
      361 agaaagtcgc tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag
      421 aacacagttt gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg
      481 ctggctatat tcgttcaaaa aatcataaaa tcagtcaacc gatttatgtt aagaaaccag
      541 ccctaaaatt agaaggaaca gaagttctca aagtctttat cgataaatac ccaagcaaga
      601 aacatgattt ctttgtcgct agtgtactcg atgtagtggg acactcaacg gatgtcggaa
      661 ttgatgttct tgaggtcttg gaatcaatgg acattgtatc tgagtttcca gaagctgttg
      721 tcaaggaagc agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg
      781 atctaagaga tgaaattacc tttaccattg acggtgcgga tgccaaggac ttagacgatg
      841 cagtgcatat caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg
      901 tttcctatta tgtgaccgag gggtctgccc ttgacaagga agcccttaac cgtgcgactt
      961 ctgtttacgt gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct
     1021 gctctctcaa tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac
     1081 atggtcgtgt ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga
     1141 cctatagcga tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaatatcata
     1201 aaattgtacc aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg
     1261 tgaaacgtgg agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag
     1321 gtaagcctgt tgatatcgtt cttcggcagc gtggcattgc cgagcggatg attgagtctt
     1381 ttatgttgat ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta
     1441 tctatcgaat tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt
     1501 cgagttttgg cttgcgcatt tatgggactg ccagtgagat tagtcaggag gcacttcaag
     1561 acatcatgcg tgctgttgag ggagaacctt atgtagatgt attgtccacg atgcttcttc
     1621 gctctatgca gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg
     1681 actattatac tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta
     1741 tgattcggga ttacggctgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag
     1801 agattgcgac ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg
     1861 aagccatgaa aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg
     1921 tatcaagtat tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga
     1981 ttcacatcac taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg
     2041 gagaaaaatc aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag
     2101 cggataaaat gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg
     2161 aaaaaggctt gaaacagtct agtcgtagtg gcagagggcg tggttcaaat cgtcgttcgg
     2221 ataagaagga agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa
     2281 aagacaagaa gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca
     2341 agcatggcaa agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat
     2401 acaatcgtag atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta
     2461 cgagctgctc gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg
     2521 ctgagcaatg ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa
     2581 cgtcgtcgta aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa
     2641 gggacaggaa tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt
     2701 cttttaggac ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt
     2761 gagcaaaatc gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa
     2821 ttgaaaatgg aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg
     2881 cctgaagctg ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc
     2941 ttgaagggag ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac
     3001 ctctttgaag caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa
     3061 gctgccacag atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt
     3121 gctaaaaaat acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg
     3181 aaacaaggga aagctttgga tttgggttgt ggtcaggggc gtaattctct ttttctagcc
     3241 cagcaagatt ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa
     3301 agcattgtgg agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct
     3361 agcattgaac aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg
     3421 gaccgcattc cagctattat tcaaaatatg caggagaaaa ccagtgttgg cggttacaac
     3481 cttatcgttt gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc
     3541 tttaaagaag gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa
     3601 aatccaggcc atttgcaccg tcgcgatgag aatggcaatc gtattcaact acgctttgcg
     3661 accttactag ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt
     3721 ttttcttttt tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct
     3781 aggggagaat tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca
     3841 gcttgtggag gccagctcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat
     3901 aaatccttaa aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat
     3961 aaggaatccc tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt
     4021 tcagaactta aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag
     4081 tgtagtccct tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt
     4141 taccaagtac tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta
     4201 cagcaaggtt ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag
     4261 gaaaaacaag ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat
     4321 tatcaaatca aggaattttc ctatggtcaa ggtagtttat tggaaatatt gcgtcttccc
     4381 tataagagac aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat
     4441 atccggcaac aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat
     4501 caaaagggag aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca
     4561 atagtgggca aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat
     4621 acctattacc aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat
     4681 cagcaatatt tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta
     4741 caaagaaatg aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac
     4801 caggcttggg aagaagcctt aaaagattta acagaacaat tggagacagt agcccagtat
     4861 gaaggccatc tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa
     4921 atggaacgcc agatagagaa gctttacgtt tatgctcata tgaagaatga ccaggataca
     4981 cgtgaagcta agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac
     5041 caagcctttt cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac
     5101 tttttagaag ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa
     5161 ggcaaggatc acgttctttc acaacgtgaa gaagaattat tggctggagc tggagaaatc
     5221 tttggttcag caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat
     5281 gtcctagacg atgatggtaa agaagttcag ctatctcatg ggacttacac acgtttgatg
     5341 gagtctaaaa aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc tacttacgaa
     5401 caattccaac acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt
     5461 gctaaagttc gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca
     5521 gaaagtgttt atgacaattt ggtagcagca gttcgcaagc atttgccact cttacatcgc
     5581 tatcttgagc ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac
     5641 acaccgcttt catctgttga atacagtttt acctaccaag aagccttgaa aaaagcagaa
     5701 gatgctttgg cagtcttggg tgaggattac ttgagccgtg ttaaacgtgc cttcagcgag
     5761 cgttggattg atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct
     5821 tacgatacca atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact
     5881 cttgttcatg aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat
     5941 gtttacgggg attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc
     6001 ttgacggaga aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat
     6061 aacttcctag atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa
     6121 cacgccattc accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa
     6181 ctctacgcag acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc
     6241 caatacgagt gggctcgcat tccacacttc tactataact actatgtata tcaatattca
     6301 actggctttg cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac
     6361 cgtgaccgct atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg
     6421 agaaaagctg gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt
     6481 gaacgccgtt taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt
     6541 tgaatcgtat agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat
     6601 tgtagacttg atgcgtcagc gtcaaaagca ggtcacaggt ttcttgaaag aattggaaga
     6661 ctttgcccgc aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt
     6721 tcttatggaa accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc
     6781 agctctcttg atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc
     6841 agaaatgatt ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac
     6901 tctcctagag ggagatgcgg tggatgtctt atctacactg acagagtctt atgatttcgt
     6961 atttatggat tctgccaagt ctaaatacat cgtctttctg ccagaaatcc tcaaacattt
     7021 ggaagttggt ggtgtggttg tcttggatga tatttttcaa ggtggtgatg ttgccaagga
     7081 tattatggaa gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa aattatttga
     7141 tgcaacctta gacaatccag aactcaccgc aacattagtg cctttaggag atggtattct
     7201 catgcttcgt aaaaatgtag cagatgttca actgtctgaa agcgaatgat tttcagaaaa
     7261 atttaagaaa aaatagtaaa atagatagag taacacttat ctcaaaggag tagacatgaa
     7321 gaaaaaatta ttggcaggtg ccatcacact attatcagta gcaactttag cagcttgttc
     7381 gaaagggtca gaaggagcag accttatcag catgaaaggg gatgtcatca cagaacatca
     7441 attttatgag caagtgaaaa acaacccttc agcccaacaa gtcttgttaa atatgaccat
     7501 ccaaaaagtt tttgaaaaac aatatggctc agagcttgat gataaagagg ttgatgatac
     7561 tattgccgaa gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt cacaagcagg
     7621 tatgactctt gaaacacgta aagctcaaat tcgtacaagt aaattagttg agttggcagt
     7681 taagaaggta gcagaagctg aattgacaga tgaagcctat aagaaagcct ttgatgagta
     7741 cactccagat gtaacggctc aaatcatccg tcttaataat gaagataagg ccaaagaagt
     7801 tctcgaaaaa gccaaggcag aaggtgctga ttttgctcaa ttagccaaag ataattcaac
     7861 tgatgaaaaa acaaaagaaa atggtggaga aattaccttt gattctgctt caacagaagt
     7921 acctgagcaa gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat
     7981 tacagcaact ggcacacaag cctacagtag ccaatattac attgtaaaac tcactaagaa
     8041 aacagaaaaa tcatctaata ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac
     8101 tcaaaaacaa aatgattcaa catttgttca aagcattatc ggaaaagaat tgcaagcagc
     8161 caatatcaag gttaaggacc aagccttcca aaatatcttt acccaatata tcggtggtgg
     8221 agattcaagc tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga gtcagggaaa
     8281 aaactctact tcaggaaaaa atgaagcaaa cattcccaca ataaaacgcg tagtacaagg
     8341 tttgtactga cccccaaaag ttagacaaat aatttatccg aaggatttag ttctgtattg
     8401 cacagggcta agtcctttta gttttacctt aattctcttg ttgttgtaat aatcaatata
     8461 gtctataatg gcttgttcca attgattaag tgatttaaat gttttctcat agccataaaa
     8521 catttcggat tttaaaatac caaagaaaga ttccatccta ccgttgtctt ggctgtttcc
     8581 cttgcgtgac atagatgctt gaattccctt actctctagg aaccgatgat aagaatcgtg
     8641 ttggtattgc cagtcttggt cactatggag aatcgtattc tcgtagtgct tctctttgaa
     8701 tgcctgttcc aacattaacg atcaatcaat ttaatcatgt acctaatatt aga
//
LOCUS       DAVLFB010000087         8326 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLFB010000087 DAVLFB010000000
VERSION     DAVLFB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.087"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..755
                     /locus_tag="VXD58_001712"
     CDS             <1..755
                     /locus_tag="VXD58_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0676369.1"
                     /translation="FHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISSTGSLVDG
                     VIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKAVTEEKFK
                     NMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQHLTTQNY
                     PDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTIAPNYVKL
                     AKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    967..1196
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1362..1439
                     /locus_tag="VXD58_001713"
                     /pseudo
     CDS             1362..1439
                     /locus_tag="VXD58_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            1986..3347
                     /gene="trpE"
                     /locus_tag="VXD58_001714"
     CDS             1986..3347
                     /gene="trpE"
                     /locus_tag="VXD58_001714"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV0676370.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNSFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGVTDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3344..3910
                     /locus_tag="VXD58_001715"
     CDS             3344..3910
                     /locus_tag="VXD58_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV0676371.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            3921..4925
                     /gene="trpD"
                     /locus_tag="VXD58_001716"
     CDS             3921..4925
                     /gene="trpD"
                     /locus_tag="VXD58_001716"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV0676372.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDVRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            4922..5689
                     /gene="trpC"
                     /locus_tag="VXD58_001717"
     CDS             4922..5689
                     /gene="trpC"
                     /locus_tag="VXD58_001717"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV0676373.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLNYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5676..6275
                     /locus_tag="VXD58_001718"
     CDS             5676..6275
                     /locus_tag="VXD58_001718"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV0676374.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6253..7476
                     /gene="trpB"
                     /locus_tag="VXD58_001719"
     CDS             6253..7476
                     /gene="trpB"
                     /locus_tag="VXD58_001719"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV0676375.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7469..8245
                     /locus_tag="VXD58_001720"
     CDS             7469..8245
                     /locus_tag="VXD58_001720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV0676376.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8217..>8326)
                     /locus_tag="VXD58_001721"
     CDS             complement(8217..>8326)
                     /locus_tag="VXD58_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV0676377.1"
                     /translation="IKLAFLLQKKKERLPFVPFLLLATCLIIFGKLLLV"
BASE COUNT         2442 a         1576 c         2024 g         2284 t
ORIGIN      
        1 ggtttcatac gctgttagat aaagtgttac ggacgtgtaa agaatttggt gttaatgcta
       61 tagttgagta ctttggagag gaggatacaa ttagtaattc aattatatca agcacaggta
      121 gcctagtaga tggtgttatt gttttttatg aaagtgtaga tgatatcaga attcagtatt
      181 taaagaagaa ccatatgcct tttcttgttt tcggtgaatc tcaaacgtct ggagtagtct
      241 atgtatctaa taataatttt caagctactt atgatatgat gaaagctgta actgaagaaa
      301 aatttaaaaa tatgtggttg cttatgggag gagaatccca tgttaataag gatcgtgaaa
      361 gaggtgtgcg ttcttttctg aatgataaaa attactttat ggatttaaaa gtcatttatg
      421 gcttatctac aattgattca gtttattctt atgcaatgca gcacttgact acccaaaatt
      481 atccggacat tatttttgtt tcaggagatg aaaaggttca gggcttgatt cgtgcgtgct
      541 atgagaaagg aattcttatt ccagatgata tttctatcat tggatttgat aatattccta
      601 tttctcagta ttatactcca gctttgtcta caattgctcc caattatgtt aaattggcta
      661 aagaaatgat agaaggtgtt ttagcaatta ttaaagggga aagtgtcaca tctgttgaag
      721 tttctcctaa atttgttagg agacagagtt tctagtattg tttaattctt gaaaatccta
      781 gtggaaaaga tggagtgttc taaaataagt tctgtttagc ctccattcag tttctattga
      841 gttggatcca atttctaaca agtgggacat gttcatgttc gatctaagga agacatggtc
      901 acattcgaat gatattttca aaaaaatcgt aaaaaagtct tgacaaagaa aaaacaaagt
      961 attaaaataa gttcaacaaa tcagaaaagt aactattttt gatcttcagg gagcctgtgg
     1021 tggttgtgaa taggtggttg gaagtagtga aagtgggctg attttgaaaa tgaatttgaa
     1081 acaatgaaaa ttcggtgcgc acaccttaca gtgcagcttg ttgttaaaca aggcagagat
     1141 gtaaagggat agtcccttta taattgaggt ggcaccgcgt taccaacgcc ctcacacgga
     1201 agtatatttt gtgtgtgggc ttttttctat ccgtcgtttg gtttatcttt tattagggcc
     1261 ttaagtcgct ttgatgaact tgagttctat ctacagctcc ttgcctacta ctaaaagcaa
     1321 acaaaaagct ggactaatac gaaaaaagct agtaatactc aatgaaaatc aaagagcaaa
     1381 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgtggat agaactgacg
     1441 aagtcagctc aaaatactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac
     1501 actgttttga ggttgcagat agaactgacg aagtcagtaa ccatacctac ggcaaggcga
     1561 cgttgacgcg gtttgaagag attttcgaag agtataaagg tgtatcgaaa taaaaatctc
     1621 ctcatcaata tctctggtcg tggaggcaag gacctagctg cgattgcaga ttacctagaa
     1681 gctaaaaaat aatagatgga aaaattacag tcttttctct cacagagagc ttgtggttgc
     1741 tgaaaacaag cagagaaagc tgtaaagttg ggttcatctg aaacgagaga agaaaacatc
     1801 attctcaagg gagtgccctt tatcgcacag cctgttacag gaggtatctg agacaggaat
     1861 gagagatagg agaaatccta taattgaggt ggcaccgcga atttcgtcct cacgcaagtt
     1921 attttgcgtg gggatttttc atacagtcac cacggactag aagtagaact gaaagggaaa
     1981 ttacaatgga acgaatcatt catggagatg tcttatcacc aatcttggct tatatgcgct
     2041 taaaggggca acacaaggtt atcttagaga gtattccgag agacaaggaa acagctcgtt
     2101 tttctatctt agcctataat cctgtttttg agattaagtt tgaaaatgga gttctttatc
     2161 aaaatggtca agtgattgat cgagatccct tggatttcct ttatgaagtg attcataaga
     2221 gtcagcacca ctcagagcta ccttttggtg gtggagccat tggttttgtg ggttacgata
     2281 tgatttcgct ttatgaagaa attggtcaaa tccctgagga tacaattggg acgccagaca
     2341 tgcatttctt tgtctatgag agctatatgg tctttgacca caagaaggag aagatacatg
     2401 tcatagagga tgctctctat agtgagcgca gtcaagaggc cttggaaaaa tccttgaacc
     2461 aagtgcttga ggaattacgc attcctgctc caaatgaatt tgaagacttg gatttatcac
     2521 cacttgactt caaaccgcat atcgctccac ataagtttga ggtaatggta gaaacagctc
     2581 gtgacttgat tcgtaatggt gatatgttcc aatgtgtact cagccagcgt ttctcagcag
     2641 aagttactgg aaattcattt gacttctaca gaaatctccg cgtgaccaat ccatccaatt
     2701 acctctattt ctatgatttt ggggattatc aaatcatcgg agctagtcca gaaagtttag
     2761 tttctgtcaa aaatggcatc gtgacaacca atccgattgc agggacgcga ccaagagggg
     2821 ttacggatga agaagacaag accttggcga ctgacctcct ctctgatgag aaggaaacag
     2881 cagaacatcg aatgttggta gacttggggc gtaacgatat tggccgcatc tctgaaacgg
     2941 ccagtgtcca agttactaag tatatgaagg tggagctctt ccgctacgtc atgcatttga
     3001 ccagcgtggt caaggggcgt ttgcttgcag aactcactgc catggatgcc ttgaaagcaa
     3061 cgcttcctgc tggaaccgtt tctggagcac caaagattcg ggcgatgaga cgcatctatg
     3121 aactggaaac ggaaaaacgg ggcgtatatg caggagcaat cggctacttg tctgcgacgg
     3181 gtgatatgga tttggccatt gccatccgaa ccatgattct caaaaatcaa agagcctatg
     3241 tgcaggctgg ggcagggatt gtctacgact ctatcgccca aaacgaatac caagaaacca
     3301 ttaacaaagc aaaatctatg actagaattg gagaactaag accatgattt tattgattga
     3361 caactatgat tcttttacct ataacttggc ccaatacatt gggaattttg cagaagttca
     3421 ggttctgaga aatgatgatt ccaagctgta tgaagaagct gaaaaagcag atggtctggt
     3481 cttttctcct ggtcctggtt ggccagttga tgctggaaag atggaagaca tgattcgtga
     3541 ttttgcaggc aagaagccga ttctagggat ttgtttgggc caccaagcca ttgcagaagt
     3601 ctttggtggt aagctaggtt tggctccaaa agtcatgcat gggaaacaga gcaatatcaa
     3661 ctttgaagcg ccatctgttt tgtatcaagg tattgaggat ggacgtgcgg tcatgcgtta
     3721 tcacagtatt ttgattgagg aaatgccaga agactttgaa gtgacagctc gttcgactga
     3781 tgaccaagcc atcatgggga ttcaacataa aaacctaccg atttatggct tccagtacca
     3841 tccagagagc attggaacgc cagacggctt gtcttctatt cggaatttta tcgagaaggt
     3901 tgtaaagtga ggaaactagg atgaaagaga ttattgaaaa actagcaaaa tttgaaaatt
     3961 tatcaggtgt ggaaatgacg gatgtcattg agcgtatcgt aactgggcgt gtaactgaag
     4021 cgcagattgc ttctctcctc ttagctctta agatgaaggg ggaaacacct gaagaacgca
     4081 cagccattgc ccaagtcatg agaggacatg cccagcatat tccaactgag attcatgatg
     4141 ccatggacaa ctgtggtaca ggtggggaca agtctttcag ttttaatatt tccacaactg
     4201 cagcctttgt cttggctggt ggcggtattc acatggccaa gcatggtaac cgctcgattt
     4261 cttctaaatc tggttccgca gatgtcctcg aagccttggg aatcaatctt gacctcaaac
     4321 cagctgaact aggtaaggtc tttgataaaa ctggaatcgt ctttctcttc gctaaaaata
     4381 tgcacccagc tatgaaatac atcatgccag ctcgtttgga attgggaatt ccaacgatta
     4441 tgaacttgac tggtccactg attcatccaa tggctttaga aacacagctt cttggaatta
     4501 gtcgtccaga actcctagaa agtacagctc aggttttgaa aaatatgggt cgcaaacgtg
     4561 ccatcgtggt tgctggacca gaagggttgg atgaagctgg cttgaacgga acaaccaaga
     4621 ttgcacttct tgaaaatggc gaaatcagct tgtcaagctt tactccagag gatttgggaa
     4681 tggaaggcta tgctatggaa gatgttcgtg gtgggaatgc tcaggaaaat gcagaaattt
     4741 tgcttagcgt tctgaaaaac gaagcaagtc cattcttgga aacgacagtc ttgaatgctg
     4801 gtcttggttt ctatgctaat ggtaagattg atagcatcaa ggaaggagtt gccttggccc
     4861 gtcaagtgat tgctagaggc aaggcccttg aaaaactcag actgttacag gagtatcaaa
     4921 aatgagtcag gaatttttag cacgaatctt agagcagaag gcgcgtgagg tggagcaaat
     4981 gaagctggag caaatccagc ctctgcgcca gacctatcgc ttggcagaat ttttgaagaa
     5041 tcatcaggac cgcttgcagg taatcgctga ggtcaagaaa gctagcccta gtttgggaga
     5101 tatcaatctc gatgtggata ttgtgcaaca ggcccagact tatgaagaaa acggagcagt
     5161 gatgatttcg gtgttgacag atgaggtttt ctttaaaggg catttgaatt atctacggga
     5221 aatttccagt caggtagaga ttccgacgct caacaaagac tttatcatag atgaaaagca
     5281 aatcatccgc gctcgcaatg caggtgcgac agttatcttg cttattgtgg cagccttgtc
     5341 cgaagaacgc ctcaaggaac tgtatgacta cgcgacagag cttggtctgg aagtcttagt
     5401 ggagactcac aatctagctg aactagaggt agcccacaga cttggtgctg agattatcgg
     5461 ggtcaacaac cgcaacttga ctacctttga ggtcgacttg cagaccagtg tagatttagc
     5521 cccttacttt gaggaaggtc gctattacat ttctgaatct gccattttca cagggcagga
     5581 tgcggaacga ctagccccat actttaacgg aattttggta gggacagctc ttatgcaggc
     5641 agaaaatgtg gcccagagaa tcaaggagtt gcagattgac aaaggttaaa atttgtggac
     5701 tatcgaccaa agaagcggtg gaaacagccg tttcagcagg agccgactat atcggttttg
     5761 tctttgcacc tagtaaaaga caggtgactt tagaagaggc agctgagttg gcaaagctta
     5821 ttcctgcaga tgtgaaaaag gttggagtat ttgtttcacc aagtcgggta gaactgctgg
     5881 aagcgattga caaagttggc ttggacttgg ttcaagttca cggtcaggtg gcagctgatt
     5941 tatttgagaa tttgccttgt gccagtattc aggctgtgca ggtagatgga aatgggcatg
     6001 tccccaattc tcaggcagat tatctactct ttgatgcccc tgtggcagga agtggtcagt
     6061 cctttgattg gggtcaactg gatacgactg gactagcaca gcccttcttt atcgcaggtg
     6121 gccttaatga agataatgta gtaaaagcaa ttcaacattt tactccctat gcagtagatg
     6181 tatcgagcgg agtggagaca gatggacaaa aagatcatga aaagattaga agatttatag
     6241 agagggtaaa gcatggcata tcaggaacca aataaagatg gattttacgg aaaattcggc
     6301 ggacgttttg tcccagaaac attgatgaca gcagttttgg agttggagaa ggcctaccgt
     6361 gaaagtcagg cagacccaag tttccaagag gaattaaacc agcttttgcg ccagtacgta
     6421 ggacgtgaaa ctcctcttta ttacgcaaaa aacttgaccc agcatatcgg cggagccaag
     6481 atttatctca aacgggaaga ccttaaccat acaggagccc acaagattaa caatgccttg
     6541 ggacaagttt ggcttgccaa acgcatgggt aaaaagaaaa ttatcgcaga aactggtgct
     6601 ggtcagcacg gtgtggcaac tgcaactgct gcggccctct ttaacatgga atgtaccatc
     6661 tacatgggtg aggaagatgt caaacgccaa gccctcaatg tcttccgtat ggagcttttg
     6721 ggagctaagg tcgaggccgt gacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca
     6781 gcccttcgtt catgggtggc taatatagat gatacccact atatccttgg ttctgccttg
     6841 gggcctcatc catttccaga aattgttcgt gacttccaaa gtgtcattgg tcgagaggct
     6901 aaacaacagt accgtgactt gacaggacaa aatctgccag atgccctagt agcctgtgtt
     6961 ggtggtgggt cgaatgccat cggtctcttc catccctttg tagaagatga gtcagtagcc
     7021 atgtatggag ctgaagcggc tggatttggt gtggatacgg agcaccacgc agctaccttg
     7081 accaagggtc gtccaggtgt ccttcacggt tccctcatgg atgtgctcca agatgcccat
     7141 ggtcaaattc ttgaagcctt ctctatctca gcaggtttgg actatcctgg tatcggtcca
     7201 gagcattctc actaccacga tattaaacgt gccagctatg tccctgtgac agacgaagaa
     7261 gccttggaag gattccaact cttgtctcgt gtggaaggga ttatcccagc tttggaatct
     7321 agccatgcta tcgcctttgc ggtgaaattg gccaaagaac ttggaccaga aaagtctatg
     7381 attgtatgcc tatcaggtcg tggggacaag gatgtggttc aagtaaaaga ccgcttggaa
     7441 gcagatgcag caaagaaggg agaagctcat gcctaagaca ctaacaaaaa aattaaatgc
     7501 tattaaagca gctggaaaag gaatttttgt tccctatatc atggctggtg accacgaaaa
     7561 aggtttggat ggtcttgctg aaacaattca ctttttagaa gatttgggtg tctctgcaat
     7621 tgaagtgggt attccctttt cagaccctgt tgcagatgga cctgttatcg aagaagctgg
     7681 cttgcgcagt ttagcccacg ggacctctac ccaggcttta gttgaaacct tgaaaaccat
     7741 tgaaacagag attccactgg tcatcatgac ctacttcaac cccctctttc agtacggtgt
     7801 ggagaacttt gtcaaagatc tggctgatac agctgtgaag ggcttgatta tcccagacct
     7861 gcctcatgaa catgccaact ttgtagaacc atttttggca gatacagata tcgccttgat
     7921 tcctttagta agcttgacca caggaattga gcgccagaaa gagttgattg aaggggcgga
     7981 gggattcgtc tatgccgttg ccatcaatgg ggtaacaggg aaatcaggca attaccgtgc
     8041 agatttggac aagcacttgg cgcaacttca tcaagtggcc gacatcccag tcttgacagg
     8101 ttttggtgta tctagtcaag ccgatctgga acgcttcaat gcggtgtcag atggtgttat
     8161 cgtcggttcg aaaatagtaa aagctctcca ccaaggagag ccgattcagg actttatcaa
     8221 acaagcagta gcttaccaaa aataattaaa caagtagcaa gtaagaggaa aggcacgaaa
     8281 ggaagtcttt cctttttctt ttgcaggaga aaggctagtt ttatag
//
LOCUS       DAVLFB010000088         8272 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLFB010000088 DAVLFB010000000
VERSION     DAVLFB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.088"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..2387
                     /gene="mfd"
                     /locus_tag="VXD58_001722"
     CDS             <1..2387
                     /gene="mfd"
                     /locus_tag="VXD58_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV0676378.1"
                     /translation="MQEFFNQFSFLKEEIERYKKMDYTIILQSSNSMGSKTLEDMLEE
                     YQIKLDSRDKTNICKESVNLIEGNLRHGFHFVDEKILLITEHEIFQKKLKRRFRRQHV
                     SNAERLKDYNELEKGDYVVHHIHGIGQYLGIETIEIKGIHRDYVSVQYQNGDQISIPV
                     EQIHLLSKYISSDGKAPKLNKLNDGHFKKAKQKVKNQVEDIADDLIKLYSERSQLKGF
                     AFSADDDDQDAFDDAFPYVETDDQLRSIEEIKRDMQASQPMDRLLVGDVGFGKTEVAM
                     RAAFKAVNDHKQVVILVPTTVLAQQHYTNFKERFQNFAVNIDVLSRFRSKKEQTATLE
                     KLKNGQVDILIGTHRVLSKDVVFADLGLMIIDEEQRFGVKHKETLKELKKQVDVLTLT
                     ATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQTYVLEKNDSVIRDAVLREMERGGQVY
                     YLYNKVDTIVQKVSELQELIPEASIGYVHGRMSEVQLENTLLDFIEGQYDILVTTTII
                     ETGVDIPNANTLFIENADHMGLSTLYQLRGRVGRSNRIAYAYLMYRPEKSISEVSEKR
                     LEAIKGFTELGSGFKIAMRDLSIRGAGNLLGKSQSGFIDSVGFELYSQLLEEAIAKRN
                     GNANANTRTKGNAELILQIDAYLPDTYISDQRHKIEIYKKIRQIDNRVNYEELQEELI
                     DRFGEYPDVVAYLLEIGLVKSYLDKVFVQRVERKDNKITIQFEKVTQRLFLAQDYFKA
                     LSVTNLKAGIAENKGLMELVFDVQNKKDYEILEGLLIFGESLLEIKEFKEENSI"
     gene            2445..2711
                     /locus_tag="VXD58_001723"
     CDS             2445..2711
                     /locus_tag="VXD58_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV0676379.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            2704..3072
                     /locus_tag="VXD58_001724"
     CDS             2704..3072
                     /locus_tag="VXD58_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV0676380.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            3192..4460
                     /locus_tag="VXD58_001725"
     CDS             3192..4460
                     /locus_tag="VXD58_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV0676381.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWCLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            4457..5734
                     /gene="tilS"
                     /locus_tag="VXD58_001726"
     CDS             4457..5734
                     /gene="tilS"
                     /locus_tag="VXD58_001726"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV0676382.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            5738..6280
                     /gene="hpt"
                     /locus_tag="VXD58_001727"
     CDS             5738..6280
                     /gene="hpt"
                     /locus_tag="VXD58_001727"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV0676383.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            6296..8254
                     /gene="ftsH"
                     /locus_tag="VXD58_001728"
     CDS             6296..8254
                     /gene="ftsH"
                     /locus_tag="VXD58_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV0676384.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAHHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         2847 a         1227 c         1717 g         2481 t
ORIGIN      
        1 ctatgcagga atttttcaat cagttttctt ttctaaaaga agaaattgaa cgatataaaa
       61 aaatggatta caccattatt ctgcagtcta gcaattcaat gggaagtaaa acattggagg
      121 atatgttaga ggaatatcag attaaattgg attctagaga taagacaaat atctgtaaag
      181 aatctgtaaa cttaatagag ggtaatctca gacatggttt tcattttgta gatgaaaaga
      241 ttttattgat aactgaacat gagatttttc aaaagaaatt aaagcgtcgt tttcgaagac
      301 aacatgtttc aaatgcagag agattaaaag attacaatga acttgaaaaa ggggactatg
      361 ttgtccatca tatccatggg attggtcaat atctaggaat tgaaaccatt gaaatcaagg
      421 gaattcatcg cgattatgtc agtgtccaat accaaaatgg tgatcaaatt tctatccccg
      481 tggaacagat tcatctactg tccaaatata tttcaagtga tggtaaagct ccaaaactca
      541 ataaattaaa tgacggtcat tttaaaaagg ccaagcaaaa ggttaagaac caggtagagg
      601 atatagctga tgatttaatc aaactctact ctgaacgtag tcagttgaag ggttttgctt
      661 tctcagctga tgatgatgat caagatgcct ttgatgatgc tttcccttat gttgaaacgg
      721 atgatcaact tcgtagtatt gaggaaatca agagggatat gcaggcttct cagccaatgg
      781 atcgactttt agttggggat gttggttttg gaaagactga agttgctatg cgtgcagcct
      841 ttaaagcagt caatgatcac aaacaggttg tcattctagt tccgacgacg gttttagcgc
      901 aacagcacta tacgaatttt aaggaacgat tccaaaattt tgcagttaat attgatgtgt
      961 tgagtcgctt tagaagtaaa aaagagcaga ctgcaacact tgaaaaattg aaaaacggtc
     1021 aagtcgatat tttgattgga acacatcgtg ttttgtcaaa agatgttgtg tttgctgatt
     1081 tgggcttgat gattattgat gaggaacagc gatttggtgt caagcataag gaaactttga
     1141 aagaactgaa gaaacaagtg gatgtcctaa ccttgaccgc tacgccaatc cctcgtaccc
     1201 tccatatgtc tatgctggga atcagagatt tatctgttat tgaaactccg ccgactaatc
     1261 gctatcctgt tcagacctat gttttggaaa agaatgatag tgtcattcgt gatgctgtct
     1321 tgcgtgaaat ggagcgtgga ggtcaagttt attatcttta caacaaagtt gacacaattg
     1381 ttcagaaggt ttcagaatta caggagttga ttccggaggc ttcgattgga tatgttcatg
     1441 gtcgaatgag tgaagtccag ttggaaaata ctctattaga ctttattgag ggacaatacg
     1501 atatcttggt gacgactact attattgaga caggggtgga cattccaaat gctaatactt
     1561 tatttattga aaatgcggac catatgggct tgtcaacctt atatcagtta agaggaagag
     1621 tcggtcgtag taatcgtatt gcttatgctt atctcatgta tcgtccagaa aaatcaatca
     1681 gtgaagtctc tgaaaagaga ttagaagcga ttaaaggatt tacagaattg ggctctggct
     1741 ttaagattgc aatgcgagat ctttcgattc gtggagcagg aaatctttta ggaaaatccc
     1801 agtctggttt cattgattct gttggttttg aattgtattc gcagttatta gaggaagcta
     1861 ttgctaaacg aaacggtaat gctaacgcta acacaagaac caaagggaat gctgagttga
     1921 ttttgcaaat tgatgcctat cttcctgata cttatatttc tgatcaacga cataagattg
     1981 aaatttacaa gaaaattcgt caaattgaca accgtgtcaa ttatgaagag ttacaagagg
     2041 agttgataga ccgttttgga gaatacccag atgtagtagc ctatctttta gagattggtt
     2101 tggttaaatc atacttggac aaggtctttg ttcaacgtgt ggaaagaaaa gataataaaa
     2161 ttacaattca atttgaaaaa gtcactcaac ggctgttttt agctcaagat tattttaaag
     2221 ctttatccgt aacgaactta aaagcaggca tcgctgagaa taagggatta atggagcttg
     2281 tatttgatgt ccaaaataag aaagattatg aaattttaga aggtctgctg atttttggag
     2341 aaagtttatt agagataaaa gagtttaagg aagaaaattc catttgatat ttttcttcta
     2401 taaaatagat aaaaatggta caataataaa ttgaggtaat aaggatgaga ttagataaat
     2461 atttaaaagt atcgcgaatt atcaagcgtc gtacagtcgc aaaggaagta gcagataaag
     2521 gtagaatcaa ggttaatgga atcttggcca aaagttcaac ggacttgaaa gttaatgacc
     2581 aagttgaaat tcgctttggc aataagttgc tgcttgtaaa agtactagag atgaaagata
     2641 gtacaaaaaa agaagatgca gcaggaatgt atgaaattat cagtgaaaca cgggtagaag
     2701 aaaatgtcta aaaatattgt acaattgaat aattctttta ttcaaaatga ataccaacgt
     2761 cgtcgctacc tgatgaaaga acgacaaaaa cggaatcgtt ttatgggagg ggtattgatt
     2821 ttgattatgc tattatttat cttgccaact tttaatttag cgcagagtta tcagcaatta
     2881 ctccaaagac gtcagcaatt agcagacttg caaactcagt atcaaacttt gagtgatgaa
     2941 aaggataagg agacagcatt tgctaccaag ttgaaagatg aagattatgc tgctaaatat
     3001 acacgagcga agtactatta ttctaagtcg agggaaaaag tttatacgat tcctgacttg
     3061 cttcaaaggt gataaaatgg aaaatttatt agacgtaata gagcaatttt tgagtttgtc
     3121 agatgaaaag ctggaagaat tggctgataa aaatcaatta ttgcgtttac aagaagaaaa
     3181 ggaaaggaag aatgcgtaaa ttcttaatta ttttgttgct accaagtttt ttgaccattt
     3241 caaaagtcgt tagcacagaa aaagaagtcg tctatacttc gaaagaaatt tattaccttt
     3301 cacaatctga ctttagtatt tattttagag aaaaattaag ttctcccatg gtttatggag
     3361 aggttcctgt ttatgcgaat gaagatttag tagtggaatc tgggaaattg actcccaaaa
     3421 caagttttca aataaccgag tggtgcttaa ataaacaagg aattccagta tttaagctat
     3481 caaatcatca atttatagct gcggacaaac gatttttata tgatcaatca gaggtaactc
     3541 caacaataaa aaaagcatgg ttagaatctg actttaaact gtacaatagt ccttatgatt
     3601 taaaagaagt gaaatcatcc ttatcagctt attcgcaagt atcaatcgac aagaccatgt
     3661 ttgtagaagg aagagaattt ctacatattg atcaggctgg atgggtagct aaagaatcaa
     3721 cttctgaaga agataatcgg atgagtaaag ttcaagaaat gttatctgaa aaatatcaga
     3781 aagattcttt ctctatttat gttaagcaac tgactactgg aaaagaagct ggtatcaatc
     3841 aagatgaaaa gatgtatgca gccagcgttt tgaaactctc ctatctctat tatacgcaag
     3901 aaaaaataaa tgagggtctt tatcagttag atacgactgt aaaatacgta tctgcagtca
     3961 atgattttcc aggttcttat aaaccagagg gaagtggtag tcttcctaaa aaagaagata
     4021 ataaagaata ttctttaaag gatttaatta cgaaagtatc aaaagaatct gataatgtag
     4081 ctcataatct attgggatat tacatttcaa accaatctga tgccacattc aaatccaaga
     4141 tgtctgccat tatgggagat gattgggatc caaaagaaaa attgatttct tctaagatgg
     4201 ccgggaagtt tatggaagct atttataatc aaaatggatt tgtgctagag tctttgacta
     4261 aaacagattt tgatagtcag cgaattgcca aaggtgtttc tgttaaagta gctcataaaa
     4321 ttggagatgc ggatgaattt aagcatgata cgggtgttgt ctatgcagat tctccattta
     4381 ttctttctat tttcactaag aattctgatt atgatacgat ttctaagata gccaaggatg
     4441 tttatgaggt tctaaaatga gggaaccaga ttttttaaat cattttctca agaagggata
     4501 tttcaaaaag catgctaagg cggttctagc tctttctggt ggattagatt ccatgtttct
     4561 atttaaggta ttgtctactt atcaaaaaga gttagagatt gaattgattc tagctcatgt
     4621 gaatcataag cagagaattg aatcagattg ggaagaaaag gaattaagga agttggctgc
     4681 tgaagcagag cttcctattt atatcagcaa tttttcagga gaattttcag aagcgcgtgc
     4741 acgaaatttt cgttatgatt tttttcaaga ggtcatgaaa aagacaggtg cgacagcttt
     4801 agtcactgcc caccatgctg atgatcaggt ggaaacgatt ttgatgcgct tgattcgagg
     4861 aactcgcttg cgctatctat caggaattaa ggagaagcaa gtagtcggag agatagaaat
     4921 cattcgtccc ttcttgcatt ttcagaaaaa agactttcca tcaatttttc actttgaaga
     4981 tacatcaaat caggagaatc attattttcg aaatcgtatt cgaaattctt acttaccaga
     5041 attggaaaaa gaaaatcctc gatttaggga tgcaatctta ggcatcggca atgaaatttt
     5101 agattatgat ttggcaatag ctgaattatc taacaatatt aatgtggaaa atttacagca
     5161 gttattttct tactctgagt ctacacaaag agttttactt caaacttatc tgaatcgttt
     5221 tccagatttg aatcttacaa aagctcagtt tgctgaagtt cagcagattt taaaatctaa
     5281 aagccagtat cgtcatccga ttaaaaatgg ctatgaattg ataaaagagt accaacagtt
     5341 tcagatttgt aaaatcagtc cgcaggctga tgaagaggaa gatgaacttg tgttacacta
     5401 tcaaaatcag gtagcttatc aaggatattt attttccttt ggacttccat tagaaggtga
     5461 attaattcaa caaatacctg tttcacgtga aacatccata cacattcgtc atcgaaaaac
     5521 aggagatgtt ttgattcaaa atgggcatag aaaaaaactc agacgtttat ttattgattt
     5581 gaaaatccct atggaaaaga gaaactctgc tcttattatt gagcaatttg gtgaaattgt
     5641 ctcaattttg ggaattgcga ccaataattt gagtaaaaaa acgaaaaatg atataatgaa
     5701 cactgtactt tatatagaaa aaatagatag gtaaaaaatg ttagaaaacg atattaaaaa
     5761 agtcctcgtt tcacacgatg aaattacaga agcagctaaa aaactaggtg ctcaattaac
     5821 taaagactat gcaggaaaaa atccaatctt agttgggatt ttaaaaggat ctattccttt
     5881 tatggctgaa ttggtcaaac atattgatac acatattgaa atggacttca tgatggtttc
     5941 tagctaccat ggtggaacag caagtagtgg tgttatcaat attaaacaag atgtgactca
     6001 agatatcaaa ggaagacatg ttctatttgt agaagatatc attgatacag gtcaaacttt
     6061 gaagaatttg cgagatatgt ttaaagaaag agaagcagct tctgttaaaa ttgcaacctt
     6121 gttggataaa ccagaaggac gtgttgtaga aattgaggca gactatacct gctttactat
     6181 cccaaatgag tttgtagtag gttatggttt agactacaaa gaaaattatc gtaatcttcc
     6241 ttatattgga gtattgaaag aggaagtgta ttcaaattag aaagaataat ctttaatgaa
     6301 aaaacaaaat aatggtttaa ttaaaaatcc ttttctatgg ttattattta tctttttcct
     6361 tgtgacagga ttccagtatt tctattctgg gaataactca ggaggaagtc agcaaatcaa
     6421 ctatactgag ttggtacaag aaattaccga tggtaatgta aaagaattaa cttaccaacc
     6481 aaatggtagt gttatcgaag tttctggtgt ctataaaaat cctaaaacaa gtaaagaagg
     6541 aacaggtatt cagtttttca cgccatctgt tactaaggta gagaaattta ccagcactat
     6601 tcttcctgca gatactaccg tatcagaatt gcaaaaactt gctactgacc ataaagcaga
     6661 agtaactgtt aagcatgaaa gttcaagtgg tatatggatt aatctactcg tatccattgt
     6721 gccatttgga attctattct tcttcctatt ctctatgatg ggaaatatgg gaggaggcaa
     6781 tggccgtaat ccaatgagtt ttggacgtag taaggctaaa gcagcaaata aagaagatat
     6841 taaagtaaga ttttcagatg ttgctggagc tgaggaagaa aaacaagaac tagttgaagt
     6901 tgttgagttc ttaaaagatc caaaacgatt cacaaaactt ggagcccgta ttccagcagg
     6961 tgttcttttg gagggacctc cggggacagg taagactttg cttgctaagg cagtcgctgg
     7021 agaagcaggt gttccattct ttagtatctc aggttctgac tttgtagaaa tgtttgtcgg
     7081 agttggagct agtcgtgttc gctctctttt tgaggatgcc aaaaaagcag caccagctat
     7141 catctttatc gatgaaattg atgctgttgg acgtcaacgt ggagtcggtc tcggcggagg
     7201 taatgacgaa cgtgaacaaa ccttgaacca acttttgatt gagatggatg gttttgaggg
     7261 aaatgaaggg attatcgtca tcgctgcgac aaaccgttca gatgtacttg atcctgccct
     7321 tttgcgtcca ggacgttttg atagaaaagt attggttggc cgtcctgatg ttaaaggtcg
     7381 tgaagcaatc ttgaaagttc acgctaagaa caagccttta gcagaagatg ttgatttgaa
     7441 attagtggct caacaaactc caggctttgt tggtgctgat ttagagaatg tcttgaatga
     7501 agcagcttta gttgctgctc gtcgcaataa atcgataatt gatgcttcag atattgatga
     7561 agcagaagat agagttattg ctggaccttc taagaaagat aagacagttt cacaaaaaga
     7621 acgagaattg gttgctcacc atgaggcagg acataccatt gttggtctag tcttgtcgaa
     7681 tgctcgcgtt gtccataagg ttacaattgt accacgcggc cgtgcaggcg gatacatgat
     7741 tgcacttcct aaagaggatc aaatgcttct atctaaagaa gatatgaaag agcaattggc
     7801 tggcttaatg ggtggacgtg tagctgaaga aattatcttt aatgtccaaa ctacaggagc
     7861 ttcaaacgac tttgaacaag cgacacaaat ggcacgtgca atggttacag agtacggtat
     7921 gagtgaaaaa cttggcccag tacaatatga aggaaaccat gctatgcttg gtgcacagag
     7981 tcctcaaaaa tcaatttcag aacaaacagc ttatgaaatt gatgaagagg ttcgttcatt
     8041 attaaatgag gcacgaaata aagctgctga aattattcag tcaaatcgtg aaactcacaa
     8101 gttaattgca gaagcattat tgaaatacga aacattggat agtacacaaa ttaaagctct
     8161 ttacgaaaca ggaaagatgc ctgaagcagt agaagaggaa tctcatgcac tatcctatga
     8221 tgaagtaaag tcaaaaatga atgacgaaaa ataaccctga gagaggctgg ag
//
LOCUS       DAVLFB010000089         8205 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLFB010000089 DAVLFB010000000
VERSION     DAVLFB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.089"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            357..2462
                     /locus_tag="VXD58_001729"
     CDS             357..2462
                     /locus_tag="VXD58_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0676385.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(2758..3240)
                     /locus_tag="VXD58_001730"
     CDS             complement(2758..3240)
                     /locus_tag="VXD58_001730"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV0676386.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(3335..4819)
                     /locus_tag="VXD58_001731"
     CDS             complement(3335..4819)
                     /locus_tag="VXD58_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV0676387.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDATRAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4972..6582
                     /locus_tag="VXD58_001732"
     CDS             4972..6582
                     /locus_tag="VXD58_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV0676388.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVEMTD"
     gene            complement(6713..8143)
                     /locus_tag="VXD58_001733"
                     /pseudo
     CDS             complement(6713..8143)
                     /locus_tag="VXD58_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT         2346 a         1511 c         1899 g         2449 t
ORIGIN      
        1 tgaggttgta gataaggaga agctgatgtg gtttgaagag gttttcgaag agtgtaaaaa
       61 aatattggtc agttaaagtc aaaactcttg accttttctg actaatgtag tatattatga
      121 acgtaaggta aatgaaagcc ttactagaac acttatttaa agtaaaatag aaagaggtgg
      181 gtctatgttt catctagaaa tcttcagaag taaagatagt ctactcctgc ttgaaaaaga
      241 aaaaccagaa atagtacgta gggtagcgat ctagctagtc taaatttttt aaaacaatgg
      301 tcaaagatag tcaatatcag taatcataac taagtaaaca aaaagaggta aagaatatga
      361 ataacaactt taataatttt aataacatgg atgatttatt taaccaattg atgggtggta
      421 tgcgaggata cagttctgaa aatcgccgtt acttaattaa tggacgcgaa gtcacacctg
      481 aggaatttgc tcactatcgt acgactggtc aattaccagg aaatgcagaa actgatgtgc
      541 aaatgccaca acaggcatca ggtatgaaac aagacggtgt ccttgcaaaa ctaggtcgaa
      601 acttgacagc agaagcgcgt gagggcaagt tggatcctgt tatcggacga aacaaggaaa
      661 ttcaagaaac atctgaaatc ctctcacgcc gcaccaagaa caatcctgtt ttggtcggag
      721 atgcaggtgt tggtaagaca gcagttgtcg aaggtctagc gcaagccatt gtgaacggag
      781 atgttcctgc tgctatcaag aacaaggaaa ttatttctat tgatatctca ggtcttgagg
      841 ctggtactca ataccgtggt agctttgaag aaaatgtcca aaacttagtc aatgaagtga
      901 aagaagcagg gaatattatc ctcttctttg atgaaattca ccaaattctt ggtgctggta
      961 gcactggtgg agacagtggt tctaaagggc ttgcggatat tctcaagcca gctctctctc
     1021 gtggagaatt gacagtgatt ggggcaacaa ctcaagacga ataccgtaac accatcttga
     1081 agaatgctgc tcttgctcgt cgtttcaacg aagtgaaggt caatgctcct tcagcagaga
     1141 atacttttaa aattcttcaa ggcattcgtg acctctatca acaacaccac aatgtcatct
     1201 tgccagacga agtcttgaaa gcagcggtgg attattctgt tcaatacatt cctcaacgta
     1261 gcttgccaga taaggctatt gaccttgtcg atgtaacggc tgctcacttg gcggctcaac
     1321 atccagtaac agatgtgcat gctgttgaac gagaaatcga aacggaaaaa gacaagcaag
     1381 aaaaagcagt tgaagcagaa gattttgaag cagctctaaa ctataaaaca cgcattgcag
     1441 aattggaaaa gaaaatcgaa aaccacacag aagatatgaa agtgactgca agtgtcaacg
     1501 atgtggctga atctgtggaa cgaatgacag gtatcccagt atcgcaaatg ggagcttcag
     1561 atatcgaacg tttgaaagat atggctcatc gcttgcaaga caaggtgatt ggtcaagata
     1621 aggccgtaga agctgtagct cgtgctatcc gtcgtaaccg tgctggtttt gatgaaggaa
     1681 atcgcccaat cggcagcttc ctctttgtag ggtctactgg ggttggtaag acggagcttg
     1741 ctaagcaatt ggcactcgat atgtttggaa cccaggatgc gattatccgt ttggatatgt
     1801 ctgaatacag tgaccgcaca gctgtttcta agctaattgg tacaacagca ggctatgtgg
     1861 gttatgatga caatagcaat accttaacag aacgtgttcg tcgcaatcca tactctatca
     1921 ttctcttgga tgaaattgaa aaggctgatc ctcaagttat tacccttctc ctccaagttc
     1981 tagatgatgg tcgtttgaca gatggtcaag gaaatacagt aaacttcaag aacactgtca
     2041 ttattgcgac ctcaaatgct ggatttggct atgaagccaa cttgacagaa gatgcggata
     2101 aaccagaatt gatggaccgt ttgaaaccct tcttccgtcc agaattcctc aaccgcttta
     2161 atgcagtcat cgagttttca cacttgacta aggaagacct ttctaagatt gtagatttga
     2221 tgttggctga agttaaccaa accttggcta agaaagacat tgacttggta gtcagtcaag
     2281 cggctaaaga ttatatcaca gaagaaggtt acgacgaagt catgggggtt cgtcctctcc
     2341 gtcgcgtggt tgaacaagaa attcgtgata aggtgacaga cttccacttg gatcatttag
     2401 atgctaaaca tctggaagca gatatggaag atggcgtttt ggttattcgt gagaaagcct
     2461 aagacagaat tttgagaata aaaaagaagg agccagctga aaaaaactgg ttccttttga
     2521 ggtacgacgg gcatgtcgtg tagtagagat gtactattct agtttcaatc tactatagta
     2581 gctcagaaat cggtacttaa acgtgctata tcaaaaccag tccttgaaaa acgtggactg
     2641 gtttcgtgtt tggattatta ctttgaacga catgcgttaa aagttagttg aaccgccgta
     2701 tgccgaacgg tacgtacggt ggtgtgagag gggctagaga ttatccccta ctcgatttta
     2761 aatcacatga cgttcaaagg catcatctga aatcccttgt tccaagatga gttttgccca
     2821 ttctttagca gagaagaggc tgtggtcctt gtagtttccg caagattcga tggttgtccc
     2881 tgggacatct tcccaagtag tagtttcagc gatttccttg agcgaatcct tgataacagc
     2941 tgcgatttta gcactggtgt gacgtcccca cataatcatg tggaagcctg tgcggcaacc
     3001 aaatggcgaa cagtcaatca tgccgtcaat gcgggtacgg atgagtttgg ctaagaggtg
     3061 ctcgatagtg tgaaggccgg cggtagggat agagtcttcg tttggttgca ccaagcgaat
     3121 atcataattg gagatgatgt ctccttttgg tcctgtttct tccccaatca agcgaacata
     3181 gggtgctttg acaatggtgt ggtcaagttc aaaactttcg acaataactt cttttgacat
     3241 ggtaaatcct ttcagttttc ttctctcatt atatcataaa ggttgctcct gagacagaga
     3301 gaaaacctct ccgaggctgg agaggttgaa atctttactt acgatataag cggtcgtatt
     3361 ggtagtatgg gtcaaaggtt acgttgatac ccagtttacg aaggacattc ttgtcttcat
     3421 cagtcaagat gatggttgag tgggcttcgc ttcctttgag gttgccgagt tcttccatag
     3481 cgcgggtagc atcaggattt tctgtagctg tgatagcaag tgcaatcagg atttcatttg
     3541 aatgaaggcg tggattgcgg ctaccgagat gatcgatttt aagaccttgg attggcttaa
     3601 caacttccgg ctcgattaat tttacttctt tagcgatgtc agctgatttt ttgatggcgt
     3661 tgatcaaggc agcggctgta gggccaaaga gttctgagtt cttaccagtg acgatttccc
     3721 catttggcaa ttcaaaggct agggctggtc caccagtttc ttctgctttt tggcgcgcaa
     3781 cgacagcaac cttacggtct gcaggtgtga taccgaggtc gttcatgagc aactcaattt
     3841 tcttgacggc agcttcgcca actttttcag ctttgaagtc aagaactgtt tgatagtaac
     3901 ggcggatgat ttcttgttta gaagcttcga cagcggcctc gtcatctgta atagcgaaac
     3961 caaccatgtt gacacccata tctgtcggtg aagcgtatgg tgattttccg agaatacgtt
     4021 ccaacatgcg tttgagcact gggaagattt cgatatcacg gttgtagttg acagtggttt
     4081 ctccataggt ttggagatgg aaggggtcaa tcatgttgac atcatcaagg tcagctgtgg
     4141 cagcttcata agccaagtta actggatgat gaaggggaag attccaaaca gggaaggttt
     4201 caaatttagc gtagccagat ttgatgccat tgatttggtc gtggtacata ttggacatac
     4261 acgttgccaa ttttccagaa ccaggtccag gagcggttac gacaatcaag ttgcgactgg
     4321 ttttgatgta gtcgtttttg cccatgcctt ctggggaaat gatgtgatcc atatccgtcg
     4381 gatatccttt gattggataa tgaagatatg aatcaattcc gtttttctca agttggttgc
     4441 ggaaggcatc tgcagcgggt tggccagcgt attgtgtaat gacaacggaa ccaacaaaaa
     4501 tccccaattc attgaatttg tcaatcaaac gaagaacttc ttggtcataa gaaatgccta
     4561 agtcgccacg tgctttggaa tgttcaatgt tgctagcatt aatggcaatc acaacctcaa
     4621 cctgctcttt caattcttgc aagagcttga ttttgttgtc aggttcataa ccaggaagga
     4681 cacgagcagc gtggaaatct tctaacattt taccgccaaa ctctaagtag agcttgccgt
     4741 caaattggtt aatacgctcc aaaatatggt cgcgttgtaa attcaaatat tgttcagaac
     4801 taaaagcttg ttttttcatt tttttacctc tggactctat tataataaaa aattggaagt
     4861 taggaaacta cggagctaaa aaagaaatta aaaagattaa gcaaacgctt gcacaaaatt
     4921 ttaaaaagtg ctatcataga ctatagatta ttaaaataat gaggtaaaca gatgcaagaa
     4981 aaatggtggc ataatgccgt agtctatcaa gtctatccaa agagttttat ggatagtaat
     5041 ggagatggag ttggtgattt gccaggtatt accagtaagt tggactatct agccaagcta
     5101 ggaatcacag caatttggct ttctcccgtt tatgacagcc ctatggatga taatggttac
     5161 gatattgctg attatcaagc gattgcaacg atttttggaa ccatggagga tatggatcaa
     5221 ctgattgcag aagctaagaa gcgtgatatc cgtatcatca tggacttggt ggtcaatcat
     5281 acctcggatg agcatgcctg gtttgtagag gcctgtgaaa atcctaatag ccctgagcga
     5341 gactactata tctggcgcga tgaacccaat gacctagatt ctatctttag tgggtctgct
     5401 tgggaatacg atgaaaagtc aggtcaatac tatctccact ttttcagcaa gaaacagccg
     5461 gatctcaact gggaaaatga aaaacttcgc cagaaaattt atgagatgat gaacttctgg
     5521 attgataaag gtattggtgg tttccgtatg gatgttattg acatgattgg caaaattcct
     5581 gacgagaagg tagtcaataa tggtcctatg ctccatccct atctcaagga aatgaatcag
     5641 gcgacctttg gagataagga tctcttgaca gtaggggaga cttggggagc aacgccagag
     5701 attgccaagc tctactctga tccaaagggg caagaattgt ctatggtctt ccagtttgaa
     5761 catatcggtc ttcagtatca ggaaggtcag cctaaatggc actatcaaaa agagctgaat
     5821 atcgctaagt taaaagaaat cttcaacaaa tggcagacag agttaggagt tgaggacggc
     5881 tggaattccc tcttctggaa caaccatgac ctccctcgta ttgtctcaat atggggaaat
     5941 gaccaagaat accgcgaaaa atctgccaaa gcctttgcaa tcttgcttca tcttatgaga
     6001 ggaactcctt atatctacca aggtgaggag attgggatga ccaactatcc gtttgaaaca
     6061 ctagatcaag tagaagatat tgaatctctc aactatgcgc gtgaggctct tgaaaaaggt
     6121 gttccgattg aagaaatcat ggacagtatc cgtgttattg gacgtgacaa tgcccgtacc
     6181 cctatgcaat gggacgagag caaaaacgct ggtttctcaa caggtcaacc ttggttggcg
     6241 gttaatccaa attacgagat gatcaacgtc caagaagcgc tggcaaatcc agattctatt
     6301 ttctatacct atcagaaact ggtccaaatt cgcaaggaga atagctggct agttcgagct
     6361 gactttgaat tgcttgatac ggctgataag gtctttgctt atatacgtaa ggatggcgac
     6421 cgtcgcttcc tagttgtggc taacttgtcc aatgaagagc aagacttgac agtagaagga
     6481 aaagtcaaat ctgtcttgat tgaaaacacc ctagctcaag aagtctttga aaaacaaatc
     6541 ttagttccat gggatgcttt ctgtgtggaa atgactgact agaatgagca aacctcaagt
     6601 ttttgaagct tgaggttttt tactatagtg gattgaaact agaatagtgc acctctgctt
     6661 ctaaaacatt gttagaaatc gatttgactg tcccgatcga tttgtcctat tcttatttca
     6721 ttttgctata cttgtagttg aggagaatta agctcctcac tagtaaactc ttcataatcc
     6781 tttttatttt tatgaagata ttgtttgaaa gatgtgagtt tccacggatg ggtttgtgga
     6841 gggatatact tgcgtctttc tttttttgtt ctggttcttg ttcaaagttt ttcgaataga
     6901 gttcatgatt tagtagctcc tttgtgtgat agattttgtc agcgatattg aggtagatgt
     6961 ctccgtcaaa tgcttttata actaatgctt tcgtctttct gatgaaatag acttcttttc
     7021 cttgttcggt agggatatag taacgatttt ggaatcggat atggtgtcca ctatcgacga
     7081 ctctctccgc cagtctagcc agaatgagat tcctttcaga ggggttagga acctcctcga
     7141 agacagagag ttttgtcttg tttccaaact gttcattaaa ggtttgaatg taggaaagca
     7201 ggaaggtatt ggcttcttcc aaggtatgaa tattgttttg ttccagttcg ataggcaggc
     7261 gagattgtag tgtctgattg agtctttcga ctctcccttt agcttgaggg atagaggttg
     7321 tctcaaggag aatccctagt tggtgacagg cgtatccaaa ctgtgtatgg gtatcgtcct
     7381 ccattttctt agagttggag gcttgatagg taaagaccgt tctcttatct gttttaattt
     7441 gaaggggaat gccgtgattg gctaagattt gttcgaggac atgatagtaa gcattcaagt
     7501 tctcttgttt atcaaaataa gcgcctagga tattgccaga agcatcatca atggctaagt
     7561 gtaagttaga tgtttgggct ccaaactagg catgagggct ggcatccatt tgaatgagtt
     7621 caccagcaaa tttctttctg ggtctactag gatgtacttt tttagggtct tcaaggaagt
     7681 tttcagccgt cggtaagatt ggattgtcta ggggttgatt caggttcagt ttagcttgtt
     7741 ttcttactct cttctttgtc tttctatggg acttaggcga caggatattt ttcttataga
     7801 gtatttttct aacagtagta tcagagagct gaattccttc ttcttcagct agcaattcac
     7861 agaaatgaag gacatttggt ttatatgttt cataggagag gtattttttt aggagacgtt
     7921 ctttgatttc atcagggatt gcatgttttg gttttcgatt tctgtttccg tgtctgaagg
     7981 cttcttttcc tttcagttga taggctagta gcagacgatt gatttgtctt tcagaaagat
     8041 tgagctcgac acaggctcgt ttctttgttt tctttccttg ggctatagct tttatcacaa
     8101 gatatttttt cgtttcattc atattcagtt ggatcctttt catatgacta ttctaccaaa
     8161 tgggacattt tcacgttcga tttactaaag acattatcac attcg
//
LOCUS       DAVLFB010000090         8044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLFB010000090 DAVLFB010000000
VERSION     DAVLFB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.090"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            473..1015
                     /locus_tag="VXD58_001734"
     CDS             473..1015
                     /locus_tag="VXD58_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV0676389.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            1015..1644
                     /gene="nth"
                     /locus_tag="VXD58_001735"
     CDS             1015..1644
                     /gene="nth"
                     /locus_tag="VXD58_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV0676390.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1855..2376
                     /gene="pyrR"
                     /locus_tag="VXD58_001736"
     CDS             1855..2376
                     /gene="pyrR"
                     /locus_tag="VXD58_001736"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV0676391.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2395..3318
                     /locus_tag="VXD58_001737"
     CDS             2395..3318
                     /locus_tag="VXD58_001737"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV0676392.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            3368..4447
                     /gene="carA"
                     /locus_tag="VXD58_001738"
     CDS             3368..4447
                     /gene="carA"
                     /locus_tag="VXD58_001738"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV0676393.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4759..7935
                     /gene="carB"
                     /locus_tag="VXD58_001739"
     CDS             4759..7935
                     /gene="carB"
                     /locus_tag="VXD58_001739"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV0676394.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2334 a         1622 c         1806 g         2282 t
ORIGIN      
        1 acaggtccat agagacttag tacgtgtttg acatactcgt aatggatttt atcatagttt
       61 cgccaagcag tgcgtgcttg attgtttagt tttaggctac ccagtccaat cagaggactg
      121 gtgggttggt aaaatttctc aagattaatt aagatactgt tgttcaactt ttctgttttt
      181 tgagctcttg catagtagtg tttagcagct cttgtagtcg aatatcatct gccgtaacct
      241 gtttacagtt ttggtagctt ttatttaact tggataggag ttgaatgagt ttcttgtctt
      301 ccatgtgagc atcctccttg atctgatgta ctctttccct atagtatagc actaaaaaat
      361 aaaactatca tagtaaatat gttaaaattt gaattttaat tacaaagttt ggtgtgctga
      421 tttgtagcct tttcatggta taatcaagag agtaaatctt tatggaggaa gtatgaaatt
      481 aaatattcaa gaaattcgta agcagtctga aggtttgaac tttgaacaaa cgttagattt
      541 agttgatgac ctgcgtgcac gtaatcaaga aattttagat gtaaaagata tccttgcagt
      601 tgggaaagta caatatgaag accgtatgta tttcttagat tatcaactat cttataccat
      661 tgttcttgct tcgagtcgca gtatggagcc agttgagtta gttgaatctt atccagtcac
      721 ggaagttttc atggaaggcg caactaacca gctagatcaa gaagttttag atgatgactt
      781 ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag agtgtatcag acaatatcct
      841 gctaaacatt cctatcaagg tcttgacggc tgaagaagaa gctggtcaag gatttgtctc
      901 aggaaatgac tggcaaatca tgacagagga agaataccaa gctcaacaag cagtaaagaa
      961 agaagaaaac agtccttttg ctggcttaca aggactattt gacggagatg aataatggtc
     1021 ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca ttgccctctt cccagatgcc
     1081 aagcctagtc ttgattttac caatcatttt gaactcctgg ttgcggtcat gttatcagcc
     1141 cagacgacag atgcagcggt aaataaggcc acaccaggtc tctttgttgc ctttccaaca
     1201 ccccaagcta tgtctgtagc gacagagagt gagattgctt cacatatttc tcgcttggga
     1261 ttgtatcgga ataaagctaa attccttaaa aaatgtgccc aacagttact agacgatttt
     1321 gatggtcaag tccctcagac acgtgaagaa ttggagagtt tggcaggtgt tggtcgcaag
     1381 acagccaatg ttgtcatgag tgtaggattt gggattccag cctttgcagt ggatactcat
     1441 gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat cagcgacgcc acttgaggtg
     1501 gaaaagcggg tcatggatat cttgccgcct gagcagtggt tagctgccca tcaggccatg
     1561 atttattttg gaagagccat ttgccatcca aaaaatccag agtgtgacca gtacccacaa
     1621 ttatatgatt ttagcaattt gtaagatgct acattgaaac tttttgcttg attttaagca
     1681 tgctttgtgc tatagtaaat gataactaaa tattccttta aacctgtccc gtgaggcagg
     1741 caaggagcgc gataaagtag aagggtgaag tctacactgt gtgcagtagg gctcgcttgg
     1801 ctataaattt caagtctcct tgtttaagga gacttttctt tttggaggtt tgtcatgaaa
     1861 acaaaagaag ttgtagacga attgaccgtc aaacgagcga ttacgcgtat tacttatgag
     1921 attatcgaac gcaacaaaga tttgaataag atcgtcttgg ctggtattaa aactcgtggt
     1981 gtctttattg cccaccgaat ccaagaacgt ttgaagcagt tagaaaatct ttcagttcct
     2041 gttgtggaat tggatactaa acctttccgt gatgatgtta aaagtggaga agatacttct
     2101 ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg tggatgatgt gctctataca
     2161 ggtcgtacca tccgtgctgc tattgataat attgtcggtc atggtcgtcc tgcgcgtgtg
     2221 agtttagcag ttctagtcga tcgtggacat agagaattgc caatccgtcc agattacgtt
     2281 ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg tagagatgac agaacttgat
     2341 gaccaagaca gagttctgat tactgaagaa gcttagaaag ttaaaggagt agccatgtca
     2401 gaaaatcaac aagcattgaa ccatgtggtg tccatggaag acctcactgt cgatcaagta
     2461 atgaaattga tcaagcgagg aattgagttt aaaaatggag cccagcttcc ctatgaagac
     2521 catccgattg tttccaatct cttctttgag gattctacac ggacacataa gtcctttgaa
     2581 gtcgcagaga ttaaacttgg attggaacga cttgactttg atgtgaagac tagttcggtt
     2641 aataagggtg agacacttta tgacaccatt ttgactctgt ctgctttagg agtggatgtc
     2701 tgtgtgattc gccatccaga ggtcgactac tatagagaat tgattgctag tccaacgatt
     2761 acgacttcca tcatcaatgg tggagatggt tcgggccaac accctagcca gagcttgctt
     2821 gatttgatga ccatttatga ggaatttggc cactttgagg gtcttaaagt tgctattgca
     2881 ggtgacttgg atcactcacg cgttgccaaa tccaatatgc agattttgaa acgcttggga
     2941 gctgaactct ttttcgctgg acctgaggaa tggagaagtc aagagtttgc agactatgga
     3001 cagtttgtaa ccattgatga aatcattgat caggttgatg tcatgatgtt tctccgtgtg
     3061 caacacgaac gccatgatag tggagctgta ttttcaaaag aagactacca tgcccaacat
     3121 ggcttgactc aagaacgtta cgatcgtttg aaagaaacag caatcctcat gcacccagct
     3181 ccaatcaatc gtgatgtaga aatagcagac cacttggttg aagcaccaaa atcacggatt
     3241 gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa tcttagaatc cgtactagcg
     3301 agtagaaacg ccaactaaaa cacaagacgt taaaagacgc cagtgagcgt ccacgagagg
     3361 tgaaaatatg acaaaaagaa ttctagtatt agaagatggc acagtttttg aaggcaaggc
     3421 cttcggagca gatattgatg taacaggcga aatcgtcttt aatacaggga tgaccggcta
     3481 ccaagaatcc attacagacc agtcttataa tggacaaatc ttgaccttta cttatccttt
     3541 ggtaggaaat tatggtatta atcgtgatga ttatgaatcc attattccaa cttgtaaggg
     3601 agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg cgcaaccaaa tgaccttgga
     3661 tgaatttttg aaagccaaga aaattccagg gatttcagga attgatacgc gtgctcttac
     3721 caagattatc cgtaagcatg gtactatgcg tgcaaccttg acccatgttg gggacagtat
     3781 ggaccatgtg acggaccagc tccaagcaac agttttaccg acagacaata tcaaacaggt
     3841 ttctactaaa acgtcttatc cagctccagg agttggtttg agcgtggtgc tagtggactt
     3901 tggtctcaag cactcaatcc tacgtgaact ttctaaacgc aactgtaacg tgacagttgt
     3961 tccttattcg acaacggctg aagaaattct ccatctcaat cctgatggag ttatgttgtc
     4021 aaatggtcca ggtaacccag aagacgttcc acaagcactc gacatgattc gcggtgtaca
     4081 aggaaaaatt ccaatctttg gtatttgtat gggacaccaa ctctttgcaa tggcaaacgg
     4141 ggctaagacc tataagatga agtttggcca tcgtggattc aaccatgcgg tacgtgaaat
     4201 cgcaacagga cgagtagact tcaccagcca gaaccatggt tatgcagtca gccgcgagga
     4261 tttgccagag cacttgatta ttacccacga agaaatcaat gacaagtcag tggaaggtgt
     4321 gcgccacaga taccaacctg ctttctctgt tcaataccac ccagatgcag ctcctgggcc
     4381 acacgacgct agctacctat ttgacgagtt tatcgagatg atggaagttt ttaaacaatc
     4441 aaactaagaa cgagtaaaag ctcgtcggag aatttatgta actgaacacg gacggaaagc
     4501 tcagaattta gagaaaccaa cttggattgt caatccttcc ttgatttctt aaaattcaat
     4561 cgctttctat tcgtcctttg tatcttattt aatgtcgccc tgagaggcga cgaatagaaa
     4621 ggagaagata cattgttcgc ggaagtgaac acgccctaag aactgtgtga aaaagataaa
     4681 catcgtcttg acgctgaagg cgtctgcgcc gagtttccta ttttcactgt gttctttacg
     4741 ggctttgtat cataaactat gcctaaacgt actgatattc aaaaaattat ggtgattggt
     4801 tctggtccga ttattattgg tcaggctgct gagtttgact acgctgggac ccaggcttgc
     4861 ttgtcgttga aagaggaagg ttatgaggtt gttttggtta actcaaatcc tgcaaccatc
     4921 atgacggaca aggaaattgc tgacaaggtt tacattgaac cgattacact tgagtttgtg
     4981 acacgtattc ttcgtaagga acgtccagat gccttgctac caacactcgg tggtcagaca
     5041 ggtctcaaca tggccatgga attatctaaa aatggtatcc tagacgagct tggcgttgaa
     5101 cttctgggta ccaaattatc tgccattgac caagcggagg accgtgacct ctttaaacaa
     5161 ttgatggaag agcttgagca acccattcca gaatctgaaa ttgttaacac agtagaagaa
     5221 gcagttgcct ttgcagcgac aattggctac ccagtcatcg ttcgtccagc ctttaccctt
     5281 ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat tgcgtgaaat cactgaaaat
     5341 ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc gttcgattgc cggttttaag
     5401 gaaattgaat acgaagtgat gcgtgactca gctgacaatg ccttggttgt ttgtaacatg
     5461 gaaaactttg acccagttgg gattcacaca ggggattcca ttgtatttgc ccctgcgcaa
     5521 accatgtcag actatgaaaa ccaaatgctt cgtgatgcga gcttgagcat tattcgcgcc
     5581 ctcaagattg aaggtggatg taatgttcag ctagctcttg atcctaacag cttcaagtat
     5641 tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag cccttgcttc taaagcgaca
     5701 ggatatccga ttgctaagtt ggctgccaag attgcagtag gtttgacctt ggatgaggtt
     5761 ataaacccag ttacaggttc aacctatgcc atgtttgaac cagcccttga ctacgtagtt
     5821 gctaagattc cacgtttccc atttgacaag tttgaaaagg gtgaacgccg tctcggtaca
     5881 caaatgaagg cgactggaga agtcatggca atcggtcgta acatcgagga atctcttctc
     5941 aaggcctgcc gctcccttga aattggggtg caccacaatg aaatacctga acttgcagca
     6001 gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc aagatgaccg tctcttctac
     6061 gtatcagaag ccattcgtcg tggctacaca cctgaagaaa ttgcagagct tacaaaaatt
     6121 gatatcttct atcttgataa actcttgcat atctttgaaa ttgagcaaga attgggtgct
     6181 catccacaag atctagaagt tttgaaaaca gccaaactta atggtttctc agaccgtaag
     6241 attgctgaac tctggggaac gactgatgat caagttcgcc aacttcgctt ggaaaacaag
     6301 attgttccag tttataagat ggtagatact tgtgcggcag agttcgactc tgaaacgcca
     6361 tatttctatt caacctatgg ttgggaaaat gaatctatca gatctgataa agaatctgta
     6421 cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg ttgagtttga ctacgcaacc
     6481 gttcactctg ttaaggctat ccaggcagct ggttatgaag ctatcatcat gaactcaaac
     6541 ccagagaccg tttcaacaga cttctctgta tcagataagc tttactttga gccattgaca
     6601 ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa agggagttat cgttcagttt
     6661 ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa aagcaggtgt gaccatcctt
     6721 ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg acctcttcga gcaagctctc
     6781 aaagaattgg atattccaca gccaccagga caaacggcta ccaatgaaga agaagcagct
     6841 cttgcagctc gtaagattgg cttcccagtt cttgtccgcc catcttatgt acttggtgga
     6901 cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt cttacatgcg aactgctgtt
     6961 aaggctagtc cagatcaccc agttcttgtc gactcttata tcgttgggca agagtgcgaa
     7021 gttgatgcca tttcagatgg taaagatgtg ctcattcctg gtatcatgga gcatatcgaa
     7081 cgtgctggtg tccactcagg tgactcaatg gctgtttacc caccacaaac cttgtcgcaa
     7141 aaggttcagg agaccatcgc agactacacc aaacgcttag caatcggtct tcactgcctt
     7201 ggaatgatga acatccagtt tgttatcaag gatgaaaaag tctacgttat tgaggtcaat
     7261 ccacgtgcca gccgtactgt tccattccta tctaaggtaa ccaatattcc tatggctcaa
     7321 gtagcgacca agctcattct tggtcaaagt ttatcagaac ttggctacca aaatggactt
     7381 taccctgaaa gcacccgtgt tcatatcaag gcacctgtct tctcctttac caaactagct
     7441 aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag gtgaagttat gggttctgat
     7501 gctactttgg aaaaagctct ctataaagcc tttgaagctt cttatctaca cttgccaact
     7561 tttggtaatg tcgtctttac catcgctgat gatgccaaag aagaagcctt gaacttggct
     7621 cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag aagggacagc agccttcttt
     7681 gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg gtgatgacga taaggatatc
     7741 ccaagttttg tacgcaaagg aagaattcaa gctatcatta acacagtagg aacaaaacga
     7801 actgctgacg aagatggtga gcaaattcgt cgttcagcca ttgaacacgg ggtgcccctc
     7861 ttcacagcgc tagatacagc taatgccatg cttaaggtac ttgaaagtcg tagctttgtt
     7921 acagaagcga tttaaataga aaaataagat atagcagact gaatttagaa cagtacacag
     7981 gaatactaaa atatttctat aaattaattt gaattttcta attgatttgt tcgcatctta
     8041 tttc
//
LOCUS       DAVLFB010000091         7902 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLFB010000091 DAVLFB010000000
VERSION     DAVLFB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.091"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..1412
                     /locus_tag="VXD58_001740"
     CDS             <1..1412
                     /locus_tag="VXD58_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0676395.1"
                     /translation="WFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGIRII
                     NTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRIISQINK
                     VIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQLLELV
                     YKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEGVA
                     QSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQ
                     ISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDI
                     KKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHA
                     KFVAETLSYYCSNNFELEVWTELELSKESLEDSPYDIIISNFIIPPIENKRLIYSNNI
                     NTVSLIYLLNAMMFIRLDE"
     gene            1774..2775
                     /locus_tag="VXD58_001741"
     CDS             1774..2775
                     /locus_tag="VXD58_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV0676396.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     KVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            2926..3843
                     /locus_tag="VXD58_001742"
     CDS             2926..3843
                     /locus_tag="VXD58_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV0676397.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAVRLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3854..4744
                     /locus_tag="VXD58_001743"
     CDS             3854..4744
                     /locus_tag="VXD58_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV0676398.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4773..6389
                     /locus_tag="VXD58_001744"
     CDS             4773..6389
                     /locus_tag="VXD58_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV0676399.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6399..7718
                     /locus_tag="VXD58_001745"
     CDS             6399..7718
                     /locus_tag="VXD58_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV0676400.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2645 a         1251 c         1507 g         2499 t
ORIGIN      
        1 gttggtttca tcgttctgaa ctagcagagt tattaaattg tacagaacgt gcagtcaaag
       61 atgatctatc ccatgttaaa tctgcttttc ctgacttgat ttttcattct tctactaatg
      121 gtatacgcat tattaatacc gatgatagtg atattgaaat ggtttaccat catttcttta
      181 aacattcaac tcatttttcg attttagaat tcatcttctt taatgaaggt tgtcaagctg
      241 agagtatttg taaagaattt tatatcagtt catcttcgct ctatcgtatt attagccaaa
      301 tcaataaagt gattaaaagg caatttcaat ttgaagtcag tctgacccct gttcaaatca
      361 ttggaaatga gagagacatt cgttactttt ttgcacaata tttttcagaa aaatattatt
      421 tcctagaatg gccatttgaa aatttttcat cagagccact atctcaattg ttagaattgg
      481 tttataagga aacaagcttt ccaatgaatt tgtcaacgca tagaatgcta aagttgctcc
      541 tagttacgaa cctatataga ataaagtttg gtcattttat ggaagtagat aaagattctt
      601 ttaacgacca aagtttggat tttttaatgc aggcagaagg aatagaaggt gttgctcaga
      661 gttttgaatc agaatacaat atctctttag atgaagaagt tgtttgccaa ttatttgtgt
      721 cttactttca aaaaatgttt ttcatagatg aaagtctctt tatgaaatgc gtaaaaaagg
      781 atagttatgt tgaaaaatct taccatctat tgagtgattt tattgatcag atttcagtca
      841 agtatcagat tgaaatggag aataaggata atctgatttg gcatctgcat aataccgcac
      901 atctgtatcg tcaagagttg tttactgagt ttattttatt tgatcaaaaa gggaatacaa
      961 tcaggaactt tcaaaatatt tttccaaaat ttgtttcaga tataaaaaaa gagctttctc
     1021 actatttaga gactcttgag gtttgttcta gttcaatgat ggtaaatcat ctatcttata
     1081 ccttcatcac tcataccaaa catttggtga ttaatttgct acaaaatcag cctaaactaa
     1141 aggttttagt tatgagtaat tttgatcagt atcatgcaaa attcgttgca gagacacttt
     1201 cttattactg tagcaataat tttgaactgg aagtttggac tgaattagaa ttatcaaagg
     1261 aatctttaga agattcacct tatgatatca ttatttctaa ttttattatt cctcctattg
     1321 aaaataagag actcatctat tcaaataata taaacacggt ctcactcata tacttgttaa
     1381 atgccatgat gtttattcga ttagatgagt aaccaaaaat aacaaaaaaa cgctatttcg
     1441 cgtttgaact gtacctcaaa agttagacag aaaaaatcta atttttgggg tgtttttgtt
     1501 aagaaaagac cttcaatcat ttaagcaaaa tgtacatcta gatttatagc cctctaaaac
     1561 tcttgctttt tttgtagaag atacgaaaaa agccataaaa ccaaggcttt tcctgtttta
     1621 tgtagatgtt tacacatgaa aattgaagta ttttcaaaag ctaattggag tgattttatt
     1681 catgatggga taaatgatct ggccaaaaat gattgagaaa tagttgctta aaaagcattt
     1741 gcatgatact atatattgaa aggaggtgat aatatggtag aacaaagaaa atcaattacc
     1801 atgaaagatg ttgctttaga agcaggagtt agtgttggaa ctgtttcacg tgtaattaat
     1861 aaagaaaaag gcattaaaga agtaactttg aaaaaagtgg aacaagcgat taaaactttg
     1921 aattacattc cagattacta tgctagagga atgaaaaaaa atcgaacaga aacgattgca
     1981 atcattgtac caagtatctg gcatcccttc ttttcagaat ttgctatgca tgtggaaaat
     2041 gaagtctata agagaaataa caaattactc ttatgttcta taaatggtac aaatagagag
     2101 caagactatc tggagatgtt gcgtcataat aaagttgatg gagtggttgc cattacctat
     2161 agtccaattg aacattactt gacgtcagga attccctttg ttagtattga ccgcacatac
     2221 tcagatattg ccattccttg tgtttcatca gataatgatg caggtggaag agaagcagct
     2281 aaacaattaa ttagtaaagg atgtcagcat ttagcctttg tgggcggaca taatacaacc
     2341 attaatgaaa ccaaacgtcg tagaatttca tttgaaaagt atgtccaaga gcataaaata
     2401 cctcttagta tttttgattt ggatgagaaa gttactgact attatggaaa gctagagagt
     2461 ttcttggaag aaaattcttc tatagatgga atttttacaa tcaacgattt tacagctttg
     2521 gatgtgattg aaatactaga aaaaaatggc aaacgtattc ctcatgatgt ccaaattatt
     2581 ggatacgatg gaattaaaat ggctggagat agagattatc tactttcaac tattgaacaa
     2641 ccattggaag aaatggcaaa agaagcggtt cgtattttgt ttgatataat tgatggaaag
     2701 actgttaatt tgcagacaat cctaccagta aaatttgttg aaggaaaaac aacaaaaaat
     2761 gaaaataagt cttgacagaa agcgctatca atgatagaat gaattcagat aaaaagattt
     2821 atttttaaaa caaaaatgaa acgtttcaaa aaaagaaata aaaagacagc gccaagcgct
     2881 atcttttata gaaaaaatga aacgtttcaa aaaaaggagg ttgctatgaa tagcaaagcg
     2941 aagcaagttt ctctttggga aagaatcaag aaacaaaaac tcttgttatt gatgactgtc
     3001 cccggtttag ttttaacctt tatctttaaa tacatcccta tgtatggggt tttaatcgca
     3061 tttaaagatt acaatccttt aaaaggaatt ttagggagtg attggattgg tttttctgag
     3121 tttacaaaat tcatatcctc tcccaacttt ggtatcttgt tagccaacac attaaaatta
     3181 agtatctatg gtttattgct tggcttttta ccaccaatca ttcttgcgat tatgctcaat
     3241 caactcttga gtgaaaaagt caaaaaacga attcagctca ttttatacgc accaaacttt
     3301 atctcagtcg ttgttattgt cggtatgatt ttcctcttct tttcagtggg aggaccaatc
     3361 aacaattttc tttctatgtt tggaatgaag gctgacttct tgacaaatcc agacttcttt
     3421 agacccttat acatctttag tggtatctgg caaggaatgg gctgggcttc aacgctctac
     3481 acggcaacat tggtaaatgt agatccagcc ttagtagaag cagttcgact ggatggagcc
     3541 aatatcttcc aacgaatctg gcacattgat attccagctc ttaagcctat tatggttatc
     3601 caatttgttt tagctgcagg tggaattatg aatgtcggat atgaaaaagc attcttgatg
     3661 cagacatcgt taaatttgcc aacttctgaa attatctcga catatgtcta taaagttggt
     3721 cttgtatcag gagactattc ttactcaaca gcggttggtt tgtttaatgc agtgattaac
     3781 gtagtattgc ttgttgcagt taaccaaatc gttaaacgca tgaataatgg tgaaggaatt
     3841 taaggaggaa agtatgaaaa attcgattat ggatacaaaa tttgatagac gtatcttact
     3901 cttaaataaa atcattattg tctttatcgt tttgatgact ttgcttcctt tactttatat
     3961 cgtcgtagca tcctttatgg atcctaaggt tctggttagt agagggatta gctttaatcc
     4021 agccgattgg actgtagaag gttaccagcg tgtattcagt gaccaatcta ttctaagagg
     4081 ttttatcaat tctctactat actcttttgg atttgcagct ttaacagtat tgctatctgt
     4141 gtttacagct tatcctcttt ctaagaaaga cttggttgga cgtcgttgga ttaactactt
     4201 cttgattgtg actatgttct ttggtggtgg tttagtccca acttacttgc tcgtaaaaga
     4261 attgggaatg ctcaatactc catgggctat cattgttcca ggtgctgtca atgtttggaa
     4321 tattattctt gctagggcct atttccaagg attgcctgaa gaattagttg aagctgctgt
     4381 cattgatgga gcgaatgact tgcagatttt ctttaaaatc atgcttcctc ttgcaaaacc
     4441 aattatgttt gttctcttcc tctatgcttt tgtaggacag tggaactcat actttgatgc
     4501 aatgatttat atcaaggatc caaacttgga accattgcaa cttgtacttc gtaaaattct
     4561 cattcagagc caaccaggtc aagacatgat tggagcacaa gcggctatga atgaaatgaa
     4621 acgtttagct gaattgatta aatacgcaac tattgtcatt tccagcttgc cattgattgt
     4681 tatgtatcca ttcttccaaa aatactttga taaaggaatt atggctggtt cacttaaagg
     4741 ataaaaaaag aaaaaataaa aggagttttc tcatgaaatt caaaacattc tcaaaatcag
     4801 cagttttgtt gacagctagt ttagcagtac ttgcagcctg tggctcaaaa aatacagctt
     4861 caagtccaga ttataagttg gaaggtgtaa cattcccgct tcaagaaaag aaaacattga
     4921 agtttatgac agccagttca ccgttatctc ctaaagaccc aaatgaaaag ttaattttgc
     4981 aacgtttgga gaaggaaact ggcgttcata ttgactggac caactaccaa tccgactttg
     5041 cagaaaaacg taacttggat atttctagtg gtgatttacc agatgctatc cacaacgacg
     5101 gagcttcaga tgtggacttg atgaactggg ctaaaaaagg tgttattatt ccagttgaaa
     5161 atttgattga taaatacatg ccaaatctta agaaaatttt ggatgagaaa ccagagtaca
     5221 aggccttgat gacagcacct gatgggcaca tttactcatt cccatggatt gaagagcttg
     5281 gagatggtaa agagtctatt cacagcgtca acgatatggc ttggattaac aaagattggc
     5341 ttaagaaact tggtcttgaa atgccaaaaa ctactgatga tttgattaaa gtcctagaag
     5401 ctttcaaaaa cggggatcca aatggaaatg gagaggctga tgaaattcca ttttcattta
     5461 ttagtggtaa cggaaacgaa gattttaaat tcctatttgc tgcatttggt ataggggata
     5521 acgatgatca tttagtagta ggaaatgatg gcaaagttga tttcacagca gataacgata
     5581 actataaaga aggtgtcaaa tttatccgtc aattgcaaga aaaaggcttg attgataaag
     5641 aagctttcga acatgattgg aatagttaca ttgctaaagg tcatgatcag aaatttggtg
     5701 tttactttac atgggataag aataatgtta ctggaagtaa cgaaagttat gatgttttac
     5761 cagtacttgc tggaccaagt ggtcaaaaac acgtagctcg tacaaacggt atgggatttg
     5821 cacgtgacaa gatggttatt accagtgtaa acaaaaacct agaattgaca gctaaatgga
     5881 ttgatgcaca atacgctcca ctccaatctg tgcaaaataa ctggggaact tacggagatg
     5941 acaaacaaca aaacattttt gaattggatc aagcgtcaaa tagtctaaaa cacttaccac
     6001 taaacggaac tgcaccagca gaacttcgtc aaaagactga agtaggagga ccactagcta
     6061 ttctagattc atactatggt aaagtaacaa ccatgcctga tgatgccaaa tggcgtttgg
     6121 atcttatcaa agaatattat gttccttaca tgagcaatga caataactat ccaagagtct
     6181 ttatgacaca ggaagatttg gacaagattg cccatatcga agcagatatg aatgactata
     6241 tctaccgtaa acgtgctgaa tggattgtaa atggcaatat tgatactgag tgggatgatt
     6301 acaagaaaga acttgaaaaa tacggacttt ctgattacct cgctattaaa caaaaatact
     6361 acgatcaata ccaagcaaac aaaaactaga ggttgattat gggagataag aaatacacag
     6421 tagaaaaagc caatcgtttt atagcagaaa ataaacatct cgttaatact caatataagc
     6481 ctgaagaaca tttttcagct gagattggtt ggatcaatga tccaaatgga tttgtctatt
     6541 ttcgtggaga ataccatctc ttttatcaat tctatccata tgatagtgtt tgggggccta
     6601 tgcactgggg acatgctaaa agtaaggact tggtgacttg ggagcacttg ccagtggcac
     6661 ttgctcctga ccaagattat gaccgaaatg gttgtttctc aggctctgcc attgtcaagg
     6721 atgatcgcct ctggctcatg tacactggac atatcgaaga agaaaccggt gtccgccaag
     6781 tgcaaaatat ggcattttca gatgacggga ttcactttga aaagattgaa caaaatccag
     6841 ttgcaactgg atcagacttg ccagatgagt tgattgctgc tgattttcgt gatccaaaac
     6901 tctttgaaaa agatggacgc tattactccg tagtagctgc caaacacaag aataatgtgg
     6961 gctgtatcgt tctactaggg tccgataacc tagtagaatg gcagttcgaa tccatctttt
     7021 taaaaggggt agaacaccaa ggctttatgt gggaatgccc agattacttc gagttagatg
     7081 ggaaagattg ccttattatg tcacccatgc gttatcagcg tgagggagac tcatatcata
     7141 acatcaactc atcgcttttg ttcacgggta aggtagattg gggagaaaaa tgttttatcc
     7201 cagaatcagt tcaagaaatt gatcatggcc aagacttcta tgcgcctcaa acattgttgg
     7261 acgatcaaaa tcgtcgtatc ctgattgctt ggatgcagac atgggggcgt acccttccaa
     7321 cccatgacca agaacacaaa tgggcatgtg ccatgacttt acctagaatt ttaagattgg
     7381 aagatggcaa actaagacaa ttccctgtta aaaaaggcca atatcaaatc caaatagata
     7441 aagattgtca ttaccactta ggaaatgata tagattatct tgaatttggt tatgatagta
     7501 atgcgcagca agtttacatt gatcgtagcc atcttattca aaaaattcta ggtgaagaag
     7561 aacaggacac tagtcgacgg tatgtagata ttgaagctaa agaattggaa gttgttctag
     7621 ataaaaattc catcgagatt tttgtcaatc aaggtgaagc aagcttgact gcaacttatt
     7681 acttaacggt gccagctgag ctatcacgaa ttgattaaaa attaagttat ttctcctaaa
     7741 gaaaaagttc tctttctaaa atagtggaaa gaggactttt tgtgttttgg gtatataagc
     7801 ttagtttatg gtatttgtaa aattggtgtt ggattatgat ttaagctagt tttctaaaga
     7861 atttgaaaaa aattttattt aagcaaaaaa aaaccttggt tc
//
LOCUS       DAVLFB010000092         7562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLFB010000092 DAVLFB010000000
VERSION     DAVLFB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.092"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(192..1031)
                     /locus_tag="VXD58_001746"
     CDS             complement(192..1031)
                     /locus_tag="VXD58_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV0676401.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(1045..1866)
                     /locus_tag="VXD58_001747"
     CDS             complement(1045..1866)
                     /locus_tag="VXD58_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV0676402.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(2003..2860)
                     /locus_tag="VXD58_001748"
     CDS             complement(2003..2860)
                     /locus_tag="VXD58_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV0676403.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(3014..3748)
                     /locus_tag="VXD58_001749"
     CDS             complement(3014..3748)
                     /locus_tag="VXD58_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0676404.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(3748..4431)
                     /locus_tag="VXD58_001750"
     CDS             complement(3748..4431)
                     /locus_tag="VXD58_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0676405.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4621..4851
                     /locus_tag="VXD58_001751"
     CDS             4621..4851
                     /locus_tag="VXD58_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV0676406.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            5080..7338
                     /locus_tag="VXD58_001752"
     CDS             5080..7338
                     /locus_tag="VXD58_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV0676407.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         2362 a         1664 c         1361 g         2175 t
ORIGIN      
        1 ttttaataaa agcagaactc tgacaaatca ctttaagtac ggatgtaaac caaatcaact
       61 ctaacaaaaa tagatttaca agagtaaaaa aacgaacaaa tttagtttct tagcgagaag
      121 aatactaaac gttcgttttt tgtgaattaa gaataatgat acaaattatc aatatttttt
      181 gtctaactat tttagacata tcttttcatt actttaggaa gacaattact aatctccgtt
      241 ggcaaaacca catactgttc ttgagctagt tcttgggcta tggctgaatg aagatgggtc
      301 gctactgcca cacgttcgta aagactagct tgcttaaact ggcctgcaaa tcctgcaatc
      361 atcccagcaa gcgtatctcc cattccccca gtcgcctgat agggaccgcc aacctgtaac
      421 tggtaataat ctgattggcc agcttgccaa atacgagtag ctgaaccttt ctccaccaaa
      481 attgttcctt gagggaagga agttagggca ctagccgttg catcttcctt ttgcttttca
      541 atcgtaattc cagataattt ttcccattct ttttggtgag gagttaggat aagctggctt
      601 gaggaaaatg acaaacttgt cctagcaaga atggtcaagg cacctccgtc tacaatcaaa
      661 atctgatttt gtcttaagcc agcaaagacc tgtttaacta gatcttctcc aaaagcatcg
      721 tctcgtaaac ccggccccag caagacaact tctgccttct ccaattgctc ttttaacaac
      781 tgctggtctt gaagagaaaa ggccatagcc tcaggtaaat ggctgtgcag ggctggaata
      841 ttttccctat ctgttccaac ggtcaccaat cctgcaccgc tttttacagc tgccaaagca
      901 gccatgatga tggcaccacc ataaggataa gttccaccaa gaaacagcag acggccatag
      961 tctcctttat gactagtacg agaacgttca ataataactt tttctagtaa ggcttgatca
     1021 atcactttca tcgttcttcc tcaactatcc tttatctgat tgttttaaaa caacttgata
     1081 gactttctta gtccactctt ctaagtcttc tccagcatag tcaagataat gcaccctatc
     1141 ttgtatttta gttggaataa tattttcaaa ttggctctta ttatgagttg gaataataca
     1201 aatatacaac tcactaaata aattcaacat gtccttaatt tttgcaacat ggtagccatc
     1261 aaaaatataa ccagtaactt taatcaaatc tgtatagata aaatgaaaac tcactccagg
     1321 aataaacttg tcttgtaatt ctttctctga aggagctcga cctaataaac gctccatcgc
     1381 taatcgataa ccagaacttg tatttgacca ccctaacatg acataatcaa aataatctat
     1441 cggatccgat gcagcatttc tactatccaa aaccaactct tcagcatctc gcctaaaggc
     1501 tttcacagca gataagagct gacctgtttt aaagatagaa atcgctgcat ctatagtcgt
     1561 agtatgacta cagtagtctg aagtcactaa cctcttatgt agatcataag tcgaggctac
     1621 tcttttatct gtaggggtaa atggtggcag aaagggatgc tgacagatgt aatctaaaat
     1681 ttcttttttt aaacaagaat cagcacactc aatacttgtt tgacgtttat gaagtttttc
     1741 gtaagctata aatttagcaa ttttttctaa ttcccactct tgaatatttg gataatcaat
     1801 tagtgctagg tagtatattc catcccattg taaactatat tccctattat aaatttttaa
     1861 aatcatctaa tcacctcact ccattataca acaaaaagga ggcgcagacc tccttttgta
     1921 atcttatatc taaaatttaa tattcatttc tgccatttta aatatagcta tagaaaatac
     1981 actctcttca gcgatttttc tcttattttc tatccaatgt ccgaagtgct gcttgataag
     2041 tttgctccat cagcatagta atggtcatag ggccgacacc tccagggact ggcgtaatat
     2101 agctagcaag tggggcaact gcctcataat ccacatcccc acagagctta ccattttcat
     2161 cacgattcat cccaacgtca atgactaccg cacctggttt gacaaagtca gcagtcacaa
     2221 acttggcacg accgattgcg accaccagaa tatctgcttt agcagccacc ttagcaagat
     2281 tatgggtgcg tgagtgggtc aaagtcactg ttgcattctt ggccaaaaga agctgagcca
     2341 tgggttttcc aacgatatta gaacgaccga tgacaaccgc atttttacct tctaattcaa
     2401 tcccatattc atgaaacatt tccataattc ctgcaggtgt tgatggaatc atgactggat
     2461 gtccagacca aaggcgtccc atatttagag gatggaaacc atccacatcc ttttctgggt
     2521 caatagccaa tagaaccgcc tcttcatcaa tgtgttttgg taatggcaac tgaaccaaaa
     2581 tcccatgcca agctggatcc tgattgtatt tagcaatcag gtctaacaat tcctcttgag
     2641 taatggtctc tggaactcgc actacttcgc tacgggaacc agccgcaaga gctgacctct
     2701 ccttgttgcg aacgtagact tggctggctg gattatcccc aaccaaaatc actaccaaac
     2761 caggcactag agctgtttct tcctttaatt ttgcagtctt ttcagccaac tgcccctgca
     2821 atttggccgc taaagctttc ccatcaataa tctgtgtcat atcacttctc ttttctttca
     2881 aatatcttcc attataccaa aaactgctag tgccctctaa cacttctttc ctaggttatc
     2941 ctatagtttc aaactataag aaaaagctct ggttgaactt ttcactaatc ttgtcttgaa
     3001 attttaaaat agattataaa accttactca agaaatcttt agtccgttgt tcttgggttt
     3061 gttcaaaaat ctgctcaggt gtcccatctt caacaaccac accgtctgcc ataaagataa
     3121 cacgatctgc cacctcacgg gcaaatccca tctcatgtgt tacgataacc atagtcatcc
     3181 ctgacttggc aaggtcttgc ataacagcca atacctcacc taccatttca ggatccaagg
     3241 ctgaagttgg ttcgtcaaag agcaaaacat ctggttccat agccaaccca cgcgcgatgg
     3301 caatccgttg ttgctgccca cccgacagac tctgtggata agcgtctgcc ttatctggca
     3361 aaccaacttt ttccaaaagc tcttgggctc tcttctcagc aacttccctg ctttcacctt
     3421 tagtcttgat aggtgacaag gtaatatttt ccatcacagt catattagga aagaggttaa
     3481 attgttggaa aaccatgccc atcttctcac gcatggcaaa caggtcattc ttcttgtccg
     3541 taatatcgac tccctcaaag ataagcttcc ccttggttgc ttcctccaac aaattcatag
     3601 agcgaagcaa ggtagatttc ccactccctg aaggaccgat gataacgaca acttctcctc
     3661 ttttaatctc gaggttgatg cccttcaata cttcattctt tccaaaagat ttatgtaaat
     3721 tttcaatttt tatcaaggtt tctgtcatta tttcttatct ccttgtccca tatgtttttc
     3781 aaaagctttc aaggctactg tcagaataga ggtcataatc aagtagtaaa atgctgcaaa
     3841 taaaagtggt gttaaaggta gataggttgt tgtagaaact gttgtagccc cattccacaa
     3901 ctccatgacc ccaatagctg ataagaggga gctgtccttg ataatggtga taaattcgtt
     3961 ccccaatgct ggcaagatat ttttgactgc ttgtggcaaa atcacataac gcatcgcatt
     4021 tttaagacga atccctagcg aataagccgc ctctagctga ccttttggaa ccgcattgat
     4081 tccggcacga acagtctccg aaacataagc accactattc atagagataa tcaaaatccc
     4141 tggaatcaga cgcgaaaaat caacacctaa aattccaatc tgaatagtcg gagcattgat
     4201 atgcataaga gcaaaggcaa tcataatttg aaccatcatc ggtgtcccac ggaaaatcca
     4261 aacgtacaag ttggccaacc aaacaagcgg tttaaacttt gaacgttgcc caaaagccaa
     4321 gacaacaccc aaaatagttc ccaaaaagat aacacagata gaaataagaa tcgtcacaac
     4381 agccccatag ttaaaataag gtaaatactt aggtaaaaaa gaaaaattca tagtcacact
     4441 gctttctaaa atagaatact gtatgattat aacatgtttt gaattaaaat ttaaattttt
     4501 tatccaattt atttaaaaat ctttaaacta gcagaattag cgagaaatct tagttttttc
     4561 tagtcaagtt gccctccttt atggtaaaat agtaacgata agaaatgaag gagaatcaaa
     4621 atggaatcac atttggttag aatcatcaac cgccttgaag caatggcaaa cgatggcgga
     4681 aatttaaaac gtaattttga acgcgaagga gttgttgttg cagaagttgc gtacagccac
     4741 gacgaagaaa acggcccaat ctttaccctt cgtgacgtag aagatcgcga aacgtatacg
     4801 tttgatagca ttgacttaat cgcaatggaa atttacgaac ttctctacta atctaaaaca
     4861 agttgggatt gtccctgcat gtctaagtaa aatccttttt gtctcacaaa taggattttt
     4921 ttatagtcca aattcacaaa aattttcatt gtaacaactt ctcaaagctt caatcttttt
     4981 acttgcttta caccccaatc ctgttataat gagagtatag aatttgacta tttttgacct
     5041 ttaaacaagt caggctaaag aaagaaatga ggtagatgta tgctttgtca aaactgtaaa
     5101 attaacgact caacaattca tctttacacc aatctcaatg gaaaacaaaa acaaattgac
     5161 ctctgtcaaa actgctataa gattatcaaa acagatccta acaatagcct cttcaaaggt
     5221 atgacggatc tgaacaatcg tgacttcgat ccctttggtg atttcttcaa tgatctaaac
     5281 aatttcagac cttctagcaa tactcctcct attcccccaa cccaatcagg tggaggttac
     5341 ggtggaaacg gcggttatgg ttcccaaaat cgtggatctg ctcaaactcc gccacctagc
     5401 caagaaaaag gcctgctgga agaatttggt attaatgtaa ctgaaattgc ccgtcgtgga
     5461 gacattgacc ccgttattgg gcgcgacgat gagattatcc gtgtcatcga gattctcaat
     5521 cgtagaacca agaataatcc tgtccttatc ggtgaacctg gtgtcggaaa aacggccgtt
     5581 gtcgaaggtc tagctcagaa aattgtcgat ggcgatgtgc cacataaact ccaaggtaaa
     5641 caagtcatcc gtctggatgt ggttagctta gttcaaggaa cggggattcg aggacaattt
     5701 gaagaacgca tgcaaaaact catggaagaa attcgcaaac gtgaagacat catcctcttt
     5761 atcgatgaaa tccatgaaat tgttggtgct ggttctgcga gtgatggtaa tatggacgca
     5821 ggaaatatcc tcaagccagc ccttgctcgt ggagaactgc aactagtcgg tgctactacc
     5881 ctcaatgaat accgtatcat tgaaaaggat gctgccctcg agcgtcgtat gcagcctgtt
     5941 aaagtcgatg aaccaacggt ggatgaaaca atcactattc tcaaagggat tcaaaagaaa
     6001 tacgaagatt accaccacgt tcaatatacc gatgctgcga ttgaagcagc tgcaactctt
     6061 tccaatcgct acatccaaga tcgcttcttg cctgacaagg ccattgacct cctagatgaa
     6121 gctggttcta agatgaactt gaccttgaat tttgtggatc ctaaagtaat tgatcagcgc
     6181 ttgattgagg ctgaaaatct caagtctcaa gctacacgag aagaagattt tgagaaggcg
     6241 gcctacttcc gcgaccagat tgccaagtat aaggaaatgc aaaagaaaaa gatcacagac
     6301 caggatactc ctatcatcag cgagaaaact attgagcaca ttatcgagca gaaaaccaat
     6361 atccctgttg gtgatttgaa agagaaagaa caatctcaac tcatccatct agccgaagat
     6421 ctcaagtctc atgttattgg ccaagatgat gcagtcgata agattgccaa ggctattcgc
     6481 cgtaatcgtg tcggacttgg tacccctaac cgcccaatcg gaagcttcct cttcgttgga
     6541 ccaactggtg tcggtaagac agaactttcc aaacaactgg ctatcgaact ttttggttct
     6601 gctgatagta tgattcgctt tgatatgagt gaatacatgg aaaaacatag tgtggctaag
     6661 ttggtcggcg ctcctccagg ttatgttggc tatgatgagg ctggtcaatt aactgaaaaa
     6721 gttcgccaca atccgtattc tctcatcctt ctcgatgaag tggaaaaagc tcacccagat
     6781 gttatgcaca tgtttcttca agtcttggac gatggtcgtt tgacagacgg gcaaggacgc
     6841 accgttagct tcaaggatgc catcattatc atgacctcaa atgcaggtac aggaaagacc
     6901 gaagctagcg ttggatttgg tgctgctaga gaaggacgta ccaattctgt cctcggtgaa
     6961 ctcggtaact tctttagccc agagtttatg aaccgttttg atggcattat cgaatttaag
     7021 gctctcagca aggataacct ccttcagatt gtcgagctca tgctagcaga tgttaacaag
     7081 cgcctctcta gcaacaacat tcgtttggat gtaactgaca aggtcaagga aaagttggtt
     7141 gacctaggtt atgatccaaa aatgggagca cgcccacttc gtcggactat tcaagactat
     7201 attgaggaca caatcactga ctactacctt gaaaatccaa gcgaaaaaga tcttaaagca
     7261 gttatgacta gcaagggaaa cattcagatt aaatctgcca aaaaagctga agttaaaagt
     7321 tctgaaaaag aaaaataaat cctatagaaa aggagtagaa aatgaaattt ttctgcttct
     7381 ttttttacta aaataactgt aatttcttga cagcttgccc tttgtccatt atgatatata
     7441 gtagactgaa tctgaaatag tacgaaacaa ttgctaaaac atttatagaa attaatttta
     7501 ctttcccaat cgatttgttc tcatcttatt tcaatctgct atagtcaatt gaaacaagaa
     7561 ca
//
LOCUS       DAVLFB010000093         7558 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLFB010000093 DAVLFB010000000
VERSION     DAVLFB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.093"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(118..1284)
                     /gene="recA"
                     /locus_tag="VXD58_001753"
     CDS             complement(118..1284)
                     /gene="recA"
                     /locus_tag="VXD58_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV0676408.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(1339..2595)
                     /locus_tag="VXD58_001754"
     CDS             complement(1339..2595)
                     /locus_tag="VXD58_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV0676409.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(2680..3696)
                     /locus_tag="VXD58_001755"
     CDS             complement(2680..3696)
                     /locus_tag="VXD58_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV0676410.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(3704..4222)
                     /locus_tag="VXD58_001756"
     CDS             complement(3704..4222)
                     /locus_tag="VXD58_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676411.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGELFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(4212..4655)
                     /gene="tsaE"
                     /locus_tag="VXD58_001757"
     CDS             complement(4212..4655)
                     /gene="tsaE"
                     /locus_tag="VXD58_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV0676412.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(4741..5361)
                     /locus_tag="VXD58_001758"
     CDS             complement(4741..5361)
                     /locus_tag="VXD58_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676413.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(5744..6607)
                     /locus_tag="VXD58_001759"
     CDS             complement(5744..6607)
                     /locus_tag="VXD58_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676414.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            7072..7296
                     /gene="pneA1"
                     /locus_tag="VXD58_001760"
     CDS             7072..7296
                     /gene="pneA1"
                     /locus_tag="VXD58_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEV0676415.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            7303..7491
                     /gene="pneA2"
                     /locus_tag="VXD58_001761"
     CDS             7303..7491
                     /gene="pneA2"
                     /locus_tag="VXD58_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEV0676416.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT         2292 a         1606 c         1253 g         2407 t
ORIGIN      
        1 aattaatttg actttcctaa taaagttgtt catatcttat ttcaagctac tatatataat
       61 ctaaaaactt gaatcaaaaa tcgaaaaagt agcggatttc tccactgctt taacagctta
      121 ttcttcaatt tcgatttcaa gttcatcgcc taagtcaagc ggaacttctt cattcactgc
      181 ttcttctttc tttggctcat cttttttgtt ttcagtatct tgttctgaaa cttcttctcc
      241 atcaatcaag ccaaatttag aacggacttg cttatcaatt tcatcaaaga tttctgggtg
      301 ctctgccaag tatttcttag cattctcaga accttgccca attttttcat ctttgtaaga
      361 ataccaagcc cctgcttttt tgataatatc caaatcgctt gcaatcttca aaagctcacc
      421 agtcttagaa attccttctc cgtacataat ttcaactacg gcttccttaa acggtggagc
      481 taccttattt tttacaacct taatcttagt ttctttaccg acattggttt ctttttgatc
      541 accagttccc ttaatttgtg tattaccacg aacatccaag cggactgaag catagaattt
      601 caaagcacgt ccgcccggtg ttgtttctgg atttccaaac atcactccaa ctttttcacg
      661 caattggttg ataaaaatgg caattgtttt ggttttattg atagaggcgc caagtttacg
      721 catggcctgg ctcatcatac gagcctgcaa accaacatgg ctatctccga tatctccatc
      781 aatttccgca cgaggaacaa gggcagcaac tgagtcgact acgacaagat caactgcacc
      841 tgagtcaatc aattttcccg caatctcaag accttgctct cctgagtctg gttgagacaa
      901 gagcaattcg tcaatattga caccaagggc cgcagcataa gctggatcaa gggcatgttc
      961 cgcatcgata aaggcagcaa tcccaccttc tttttgcgct tgtgcaactg catgaagggc
     1021 aaccgttgtc ttaccagatg actctgggcc atagatttcg atgatacgtc ccttaggata
     1081 accacctgag ccaagggcaa tgtcaagagc taaagaacct gagctcatca cttgcacctt
     1141 ttgctccgca cgttcaccca aacgcatgat tgatccttta ccaaagtctt tctcaatcaa
     1201 tttaagagca tcattcaagg ccttttcacg ttctgcccca aatttttttg aaatttcttc
     1261 taattttttt ggttttttcg ccattctatt ctcctacatt ctaatgatcc tcagacctat
     1321 ctactattat atcaaaagtt agtcacttaa taaagccttg cgaactaggt taaaggcatg
     1381 cataaccgca atatgacgta catctgctcg gcttctgcct ccaatattaa ccttgataac
     1441 ctcagttcct tgctcttgcg ccaagcctat gaagactgtc ccaactgggt gtccttctag
     1501 gctatctggt cctgccactc cggtcaaact aattccaaaa tcagactggg tcttgcttcg
     1561 tgcctgctca gccatcttct gagctgtaaa ttcagacacc acaccatgtt cttccaaatt
     1621 cttggcagga atatccaaca tccttgattt ttcctccaag ctataggtca caaaaccacc
     1681 cttaaatata cttgaagctc cagaaaaatt cgccacggta gcttggaaaa gacctgccgt
     1741 taaactctct gcagccgcga tggttttccc ttgccttttc agttcttcta ccacaatgct
     1801 ggctaaacta gtttcttccc cataaccata gcaaaagtct cgtaaagaaa ttccttcgaa
     1861 agtctggcag tccaagattt gattttccaa gatatccagc gcttgattcg cctcttcttg
     1921 actgctagcc tttgttgaca gacgtagagt gacttctcct gtcttggcat aaggggccaa
     1981 ggtaggatcg atctgattat caattaaatc agccaaaatc gtaaccaact ggctctcgcc
     2041 aatcccaaag aaacgaagaa ctcgggaata cagcttgctc cctgtcatca acttgggtag
     2101 aagttggttt aagaccatgg gtttcaattc acttggcgga cctggaagga cgacataggt
     2161 cactccgtct acttctaatt ttcctccaac agccagtcct gtttcgtttg gcagtggaat
     2221 cgctccttct acaatttgag cttgtctttc gttattcggt gttcgggcat agtctggtcg
     2281 cagggcaaaa aagatatcca acttctcctg agcctgagga tcaaagacta atgctttccc
     2341 taaaaattta gctagggttt gtttggttag gtcgtcctca gttggcccca aaccgcctgt
     2401 caaaatcacc agactgctac gttgactggc aatctcaagc aaagacaaga gacgaacttc
     2461 attgtctcct acagccgtct gaaaatatac atctacccca atctcagcta gtttttccga
     2521 caaaaactgg gcattggtgt tgacaatctg tcctgtcaaa atctctgttc caacagcaat
     2581 gatttctgct ttcatgtttc ctcctaccta tctattcgta tttttttgaa aaaatcgcag
     2641 gaattttcct acgattgatt tttttatttg tatcaaaagt taattatctt catcaccaac
     2701 aggtgctctg ccaaataaat cttcaaataa aaccgcattg gtttcaagct gagtaacttc
     2761 ttcttgtccc aaagaacgtc ggagtatatt ttgcatttcc aacatatgtg ctctcgaaac
     2821 aatctggtaa gaaacacctt gaagtatctc tccttcaccc tgcaactgct gagtttcaat
     2881 ggttttaaat gaatctttat agcctagcaa gttagggata ctttttgcag acaaatcaat
     2941 attggtctgc atattgtcac tcaaagcttt tagaatctct tgataatgac caacgctatt
     3001 taaactgaga gctttttcca tgactttttg aataacttca cgttgacgtt tttgacgacc
     3061 ataatccccc tcaggatctt ggtaacgcat tcgtgcatag actagggctt cttctccccc
     3121 aatatgttgc tccccaacac cgatagaaat agtattaaat tcttcttggt cactgataga
     3181 aattgggaaa cctaggatat tattgactgt aatacctcct actgcatcca ctagtttttg
     3241 caaccctctc atattgacca tcacatagcg atcaatatgg atattcatca ttttttgaat
     3301 ggtttctata gcaagctctg ctccaccatc tgcatatgct gagttcagtt tcgcttcatg
     3361 agcctgacca ttccctgatt caatgcgcgt cagaatatcc cgctctaaac tcatcattgt
     3421 tgtttttttc gttttaggat tcactgtcat caagatcatg ctatcacttc taccgaccca
     3481 agtttcagtt cgttcaacat ttccggtgtc cactcccatt aacagaatgg ttagaggttc
     3541 agtcgcttca ataaccttgg tttcttcacc gattttttta taggttttag ctaaggtttc
     3601 tgtcccttgt tgataaatag tataagcaaa aacacctact cctactacag ttacagaaag
     3661 taaagctagc accattccaa taattttttt aaccatattt ctactaacct atcagtttac
     3721 ccatcaagta aacatcgata aatttccctt cttctatata tgccccacgc tcttggctac
     3781 cttcaatgac aaagccatgc ttttgataaa gatggactgc tgcttgatta cgagtttgga
     3841 cagtcagttg gagacgacgc agaatgccac ttgcttgtgc ccactctatc gcttcttcta
     3901 gcaacaaact tcccaagcca ttattccaat atctttttcc aatcacaatg aagagttctc
     3961 caatatgacg gactctctta cgctggtcag ctgtaatatt tacaatacca gcaattttgc
     4021 catttaagaa tgcaagtaag gttatctgat tgtccgaact agcttgcttg ttgaggaata
     4081 tttccatctc ctcactagtc aagagaatac catctccgtc taggctggta aagtctgtct
     4141 ccaaactcac acgatttaaa aaggccacta attcagctgc atctttgggc tctgcttccc
     4201 taatgagcaa ttcatactcc atattgaagc tcctctaaca atttctcagc acgcaaaccc
     4261 tttgcctgaa aatttaaacg gcgtccatct gcttctttta gaatttccaa ttctaaataa
     4321 gcatctggca aggcatctcc taagagattg ccccactcaa taacagtcac gccgccacca
     4381 aagataaact catccaagtc gatagaatca gcatctcctt caatacgata aacatctagg
     4441 tgataaagtg gaagtcgacc ttcatactct ctcacgatag tataggtggg acttttaatc
     4501 atttgagaaa tctgtaatcc ttttgcaagt cctttagtaa aggtcgtttt acctgcaccc
     4561 agttctccag ttaagattaa aacatcattc tttgctaata gatggcccaa acgctcccct
     4621 aaggcttgca actcttcttc attttttgtg tacatactct tattatacca aaaacttttc
     4681 ttttgtgtct attttcctac taaacttatc atcataacat ccataaaaaa caggctttct
     4741 ctaaaagaaa atgagcgtaa caatgaccaa tacaagatct cggaaaatat gaccataaaa
     4801 ggaaacttcc ttcttaaccg aatttgggac aagataggct gcaaaaaaca agcccagtcc
     4861 aatataaatc agaagtgaga caatggtcat tggatttctt aagaaaagaa gtgttgctaa
     4921 aatagtcacc aacactgtct tttttctgtc tagcatagca agaaaatcgc gcacgtattt
     4981 tttcaagggt aaaaaaatca gcaaatctag cccaaatagg aaaaagaagg atggcaataa
     5041 aaagtcaact aattcttgct gcagcgtatt tttgatgaac aagttatctg acaaaacaag
     5101 aacagctcct aacaaattaa ttaagagtaa catactgtaa aaaagcttca cagacttctt
     5161 actggctagg acactatgga cttcttgctt acgggtataa agataattta ctccagcaca
     5221 gattcctgaa acgaaaacca tgcttccgat gaaaaaaact gtactttgtt taaaggacaa
     5281 gatgcattcc ttccatagga aacagctact caaactgatt tgaattaaag ctaacaaaaa
     5341 taagattctc attgatttca tcttctctct cccttcctac caatcattat actaggagaa
     5401 aagagagaac tgtttctaat cttctcaaat gtctctttaa gacgctaaac aaacactaga
     5461 gactaatact caatgaaaat caaagatcaa actaggtagc tagccacagg ttgctcaaaa
     5521 cactgttttg agattgcaga tagagctgac gtgatttgaa gagattttcg aagaatataa
     5581 atttgaaatc atgaaaatcc gtcaaacggg tggttgtttt gtctcgcacc tcacggagcg
     5641 agacggactc agagtcacat aattataagg ctgatagtat taatctaact atcagcctta
     5701 caggttattt aacgtttcag aaaaactata atgtcaagat taactaaaca gtatctaatt
     5761 ccttcaaata attttctatc ttcatcaaca ttaaaggatt gttataaatc ttacataact
     5821 ctcttgcttc tatataataa tttttgactt gttctctgtc tagaaatttg gctccagcat
     5881 ttcctacaag aataagtaga ggagccaatt ggtagcttgt ctgtctttgt ttacagagtt
     5941 caatcgtttc aagagcttct tggatggctt cattatattt ttcctttgat actaggtagt
     6001 gagcgtagtt gtaacgaact ctgatgtagc caaataaaaa ctcttgatgg tccaaatttt
     6061 ttgtctgata caactctatt aaatgagagt agtttgcctc atattcttgt tcacgaccca
     6121 ctaaggaata gaaattagat agagtattca acgcctttaa ataaatcaga gtatttgaag
     6181 agacttttaa taatatattt tccaatgacg aaattgcctc acacttactg tcatattgat
     6241 agaagtcaat tatagattta atccattcaa ggtaagttcg gtcttctaat gttagaaaag
     6301 tgcttcgttc tacctctatt ttataaagat attctaaatc gtcataattt ctgtcatcta
     6361 ataggcgagc agatagatgt ttgaaattag agaggttaga cttaacttcg atttgttcat
     6421 tgaaaaagta atccaaaggg acttcaagtc gttgagagag tttgaataac aagtctgcgg
     6481 agggaataaa atgacctctt tcaattttac taatctggct ttgttcacaa attccttccg
     6541 caagagtttg ttgggagagt ctcaactctt ttcttttcaa tctgaatttc tctgcgagtg
     6601 tattcattaa agataataaa ccttcctatt tgcttaattt cattataaag tttttagttc
     6661 aaaatagcaa gttaaaggaa taaataattc tttatggtaa taaacaaaaa aaaaagttaa
     6721 aaattacata aaaatacttg actaaataaa atatatctgt tagaataaaa acaaggaaaa
     6781 agaaaggggt ttcattgcat gagaaaaaaa cgtggaatta aaaaattagt tacatttgca
     6841 ttgctaggtg tttttatgtt tagtaataca attccttacc aacagtttat tcagaagaat
     6901 aaacaattgg agattcgagt gtaatcgcaa aagaagtcca atggtcttga tgttgggaag
     6961 gctgattaaa acccgatatt ttgcacgaga atgagagaat tataatttat ctgaaaaaac
     7021 gaaaaatata gagcaactta tttaactttt tataaggagg agttattact tatgaccaac
     7081 tttaattcaa acgaaaaatt ttgtggaaaa agtttaaaat cattgtctgc tgatgaaatg
     7141 agtttaattt atggtgcatc tgatggtgca gagccacgtt ggacgcctac tcctattatt
     7201 ttgaaatcag ctgcagctag ctcgaaagta tgcattagtg cagctgtttc tggtattggg
     7261 gggctagtta gctacaataa tgattgtttg gggtaaatta aaatgaaaaa tgattttgta
     7321 atcggaaaga gtttgaaaga attatctctt gaagaaatgc aattggttta tggtggcaca
     7381 gatggtgctg accctagatc aacaattata tgttctgcta cattaagttt tattgcatca
     7441 tatttggggt cagctcagac tcgttgtggc aaagacaaca agaaaaaata aaatattcaa
     7501 ttttaaaaat aaaagttgct ctttattttc ctctctcgta actaataatt ttgagaga
//
LOCUS       DAVLFB010000094         7117 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLFB010000094 DAVLFB010000000
VERSION     DAVLFB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.094"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(258..1232)
                     /gene="pta"
                     /locus_tag="VXD58_001762"
     CDS             complement(258..1232)
                     /gene="pta"
                     /locus_tag="VXD58_001762"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV0676417.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1276..2172)
                     /locus_tag="VXD58_001763"
     CDS             complement(1276..2172)
                     /locus_tag="VXD58_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0676418.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2169..2987)
                     /locus_tag="VXD58_001764"
     CDS             complement(2169..2987)
                     /locus_tag="VXD58_001764"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV0676419.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(2971..3642)
                     /locus_tag="VXD58_001765"
     CDS             complement(2971..3642)
                     /locus_tag="VXD58_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV0676420.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3770..4339
                     /locus_tag="VXD58_001766"
     CDS             3770..4339
                     /locus_tag="VXD58_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV0676421.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4452..5411
                     /locus_tag="VXD58_001767"
     CDS             4452..5411
                     /locus_tag="VXD58_001767"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV0676422.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5421..6536
                     /locus_tag="VXD58_001768"
     CDS             5421..6536
                     /locus_tag="VXD58_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV0676423.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            6541..6888
                     /locus_tag="VXD58_001769"
     CDS             6541..6888
                     /locus_tag="VXD58_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV0676424.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            6896..7111
                     /locus_tag="VXD58_001770"
     CDS             6896..7111
                     /locus_tag="VXD58_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0676425.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
BASE COUNT         2196 a         1529 c         1333 g         2059 t
ORIGIN      
        1 acttcaaccc actacagttg acaaagagcc aaaaaattct cttttcatac tcatttaaaa
       61 tcaaaaaggg aatttatcgt tttacggata tgtatattac agatttctat aggtcgtgta
      121 tagacaggat atcatcgctt ggaagttttc ctttttgaca ccaatcagta atttagttct
      181 caggaattaa tggcagtgct tttcgtacat agtttcataa ctacagtata gcatagtata
      241 tcactttata gttttcacta ttgatgaact gcttgagctg ctgtgatgag ggtcaacttg
      301 taaacatcat ctgcattaca tccacgagaa agatcattaa ctggcttgtt taaaccttgc
      361 aaaacaggtc ctacagccgc aaagccaccc aggcgttcag ccatcttgta accgatattt
      421 cctgcctcga tacctgggaa gatgaagaca tttgcttgac cagctactgt acttcccgga
      481 gctttcagag ctgcagtttc gggaacaaag gccgcatcaa attgcaactc accatcgatt
      541 tcaaggtcag gacgcaagtc gtgagcaatt ttagttgctt caacgacctt atcaacgctt
      601 tcaccaaacc ctgaaccttt agtagaatag ctcaacatgg caattttagg ttcgatgcca
      661 aacatcttag ctgtgattgc tgagttgatg gcaatttcag ccaaggcttc tgcatctgga
      721 ttgatattaa tggcacagtc tccaaatagg taacgttccg taccacgaac catgaggaag
      781 gctcctgaag tacgagttac atttggacga gttttgatga tttgtagagc tgggcgaact
      841 gttgaagctg ttgagtgaat cgctcctgac accattccat caaccaagcc caagtaaacc
      901 aacatcacac caaagtagtt gacatcttca accaaaacct tgcgtacatc ttcttcagtc
      961 attttgccct tgcgacgctc caccaaggca gaaaccattt cttcaaattg aggataatgt
     1021 tgagggtcga tgacctcata accatccatg attccttcaa tttcaagata aattttaatt
     1081 ttttcaggat ttccaagcaa aacaggaatc acttctgttt cttttactaa gcgttttgtt
     1141 gcttgaagaa tacgaggctc ttccccttca gggagaacga tacgagcatt tttaccaaca
     1201 aggttggctt tgagactttc aaaaacttcc atgagttttc tcctttaaga taataattat
     1261 actctgagac agtttttata gagtattagt tgataactgg gtaataaggt tactgaaatc
     1321 atccggcaag ggactttcta actgcaagtc ttgctctaaa aatggatgat aaaaggatag
     1381 gtaatggcaa tgcagagcct gacgttgaat accatcttcc agactaccac catacaaatc
     1441 atctcccagc aaaggaaaac cgatatgaga aaaatggact cggatttgat gggttcgacc
     1501 agtgtgcagg tgaatataga ccaagtgaat atttccataa gaagctacaa tcttgtatga
     1561 agtatgggca tactttccgc ctttagccac tcgtctggta ataatggaat cttcatcacg
     1621 cgcaatcgga gcaataattt ccccttctgg ttccaaatgt ccatctccct taaccaaagc
     1681 aaagtagcgt ttctcgatag atttcttctg caactgcttg tctaatcgtg catgggcata
     1741 accgtgcttg gcaaagagca tcaagccaga agtgtcccta tctagtctgg taacaatgtg
     1801 aacctgctga ttttcataat tttgcttgac atagtaaccc ttgataaaat tggcaatggt
     1861 attagagtga ttaacactag gaatagaagc cactccatag ggtttattca agactagaaa
     1921 gtggtcatcc tcatagagaa tatctaatgg aagctcaata gcctccaagg tttcaaagcc
     1981 tttctcagcg ggaatgtcaa tggtaacgta gtctccaacg tccaatagat acgttgcatt
     2041 ttgcggttga ttattgacca gaatagctcc acctcgaaac ttaatcttgg ccagcaatcc
     2101 cttagaaacc tcgtgctttt ttaagaaggt cttaaccttg acatgttcat ctgcgataaa
     2161 ttcaaacctc attcatccac ctcaccgata aaggcatcct taacacggtt ccagaaactg
     2221 gtatggctag gagtcgcgac aaagtgaatc ttatgatggg cgatttgata ctcaatacgc
     2281 tcaatattac ggaaagaata aacgctattg tcaaccgaaa tagtatgata atcgtttctt
     2341 gttggaataa gttcaatctt atccttctta ggcacaataa tggaagagcc cagtgttcga
     2401 tagacacgat tattaaggct ggcaatttcc gttaattgca aagcttcaat ggtagggtgt
     2461 aaaacagcac cgccaagaga cttgttatag gcagtactac cagtcggtgt cgaaactgtt
     2521 agcccgtctc cacgaaaacg ttcaaaggga acaccattta ttacaatatc tgccaccatg
     2581 gttcgatcag acctgcggat gctggcttcg ttgagtgctc tgaaaatctt aacttcacca
     2641 ttttcaagaa agaccttcac attcagaaca gggtaagaaa cccttgcccc agtatctagc
     2701 tgcaaattag tcactagctt gtccaactca aaatcacgat aatctgtata gaagcccaaa
     2761 tgtccagtgt gaagaccgat aaagcggacc ttctcaagct gattttcgta cttatgaaag
     2821 gccgacaaga gcataccatc cccgccaatg gaaatgacaa tatccggatt ggtatcattg
     2881 agtataaact gatttctctt caaacgatct cgcaattcat acaaaaccct ttgactctgc
     2941 ggttttctat tggctatcag atcaattcgt ttacctgtat tcttcatctg tatcgtcact
     3001 gtttcctaca ccgtcattta attttctact caaaggatca aaaagtgcct gagcttcttg
     3061 gatatcatca cgaatttcac ccatttcttc atccaactga tgggcgattc tagctgtaat
     3121 ttccagtcgc ttcttaatct catctgggaa atccccttgg tacttgtagt tgagagaatg
     3181 ttctatcgtt gcccagaaat tcatggccaa agtacgaatt tgaatttctg ccaaaatagt
     3241 cttaactcca ttgatggtat caaccgtata ttctactacc acatgatagg aacgatagcc
     3301 tgatgctttt ctatgagtaa tgtaatctcg ctcctgtatg attcgcatat cctgacgctt
     3361 gtgcaaaata tccactactt ccttgacgtc atctacaaac tgaaccatca cacgtaagcc
     3421 agcaatatcc tgcaaatcgt gttccaaggt cgcataagta atgccacgac gagccatttt
     3481 ttctttgatg ctctcaattg gcttaactcg accggtcaca aactcaattg gagaatgctt
     3541 attttgctta cgatattgct tacgaatacc acgaagttta atctttaact caccaacagc
     3601 ttgaatgtaa ggatctagaa attcttccca ttctaaggtc atatattctc ccttgttctc
     3661 atattatccg tcaaaaatat ctttgatttt ttctccagat tcatatacaa taaatacaat
     3721 gcttattata ccataaatcc gctttcaacg ataaaaaaag gtaagaaaaa tgaaacattt
     3781 agaaattgaa ttgaaaacac tattgaaaaa agatgaatac aatcgtctaa aagaccagtt
     3841 cacaggtgtc actcctgttc ttcaaacaaa ttactacatc gacacgcctg attttgaact
     3901 gcgagaaaag aaagtcgcta tgcgcattcg aacctttgaa gactgggctg agttgacact
     3961 caaagtcccg cagagtgttg gaaacatgga atacaaccaa aaattgcaac taaaagatgc
     4021 tgagaactat ctgagtaagg aagaacttcc tcaagggcta gtactggatg aattggcgaa
     4081 acatggtatc caaagtaaga actggcaggt tcttggttgt ctaacaacgc ttcgctatga
     4141 aatgaaaaca gctattggtc tcatggcact ggatcagagc cgatactttg atataacaga
     4201 ttacgaattg gagcttgaag tggaaaatca tgagcaaggc aaacaggatt tccgacaatt
     4261 tttagagaaa aatcagattt cctaccaaaa agctccttca aaattggttc gatttgtcaa
     4321 aagtatgaaa aatagctgaa ataatctcca ttttttggta aaatagaaaa gataaaaata
     4381 cacaaatccc agttcatata tgtaaggtaa atataactag gatttataaa gtttacagag
     4441 gacggtctat aatgtcagat agaaaaaaca tgaaactttt cgcactcaac tctaaccaag
     4501 agattgcaca aaaaattgcc caagctgttg gtgtcccact tggaaaacta tcatcacgtc
     4561 aattttcaga cggagaaatc caagtaaata tcgaagaaag tgtccgtggt tatgatgttt
     4621 acatcatcca atcaacaagt ttccctgtca acaaccacct aatggaattg ttaatcatgg
     4681 tcgatgcttg tgtgcgtgca agtgcccaca gtatcaacgt tgtccttcca tattttggct
     4741 atgcacgtca agaccgcatt gcttgtcctc gtgagccact tacagcaaaa ctagttgcca
     4801 atatgctggt taaggctgga gttgatcgta tcctgactct tgatttgcat gccgttcagg
     4861 ttcaaggttt ctttgatatt ccagtggata atcttttcac tgttccccta ttcgcaaaac
     4921 attactgcga taaaggattg cttggttcag atgttgttgt cgttagccct aaaaattcag
     4981 gtgtcaaacg tgcgcgtagc ctggctgaat atcttgatgc tcctatcgcc attatcgact
     5041 accctcaaga cgatgcaact cgtaacgaag gttatattat tggtgatgtt gaaggtaaga
     5101 aagctatctt gattgatgat attttaaata caggacgtac cttctctgaa gcttctaaaa
     5161 tcgttgaacg tgaaggagct acagaaattt atgctgtttc tagccacggt ctcttcgtcg
     5221 agggagctgc tgagcttctt gacaatacta atattaaaga aattcttgtg actgattcag
     5281 tagcaacaaa agaaaaaact cctaaaaatg tatgctacat cactgctagt gagttaattg
     5341 gtgatgctat cgtccgtatt cacgaaagaa aaccagtcag cccactcttt gcctacaata
     5401 aaaagaaata aggtgattct ttgatttatt tggacaatgc tgcaacgact cctatgtcag
     5461 cagttgctat ttcagctatg accaaggtta tgcaagaaac ccacggaaat ccttctagta
     5521 ttcatggtca tggtcgtcaa gctggcaaac tcttgcgaga agcccgtcag gaactagccc
     5581 agttactaag gacaaaacct caacatatct ttttcacttc tggtgggact gaaggcaata
     5641 atactaccat cattggctac tgtcttcgtc accaagaaca aggaaaacat atcatcacaa
     5701 ctgccatcga gcaccatgct gtccttgaaa caattgatta cttggttcaa cactttgggt
     5761 ttgaagcaac cattatccag ccagaaaatc aagaaatcac agcccagcaa attcaaaagg
     5821 ctttacgtga cgatacgatt ttggtttcta ccatgtttgc caataatgag acaggaaacc
     5881 tactgcccat cgctgaaatt ggccaaatac tcaagcaaca ccctgctgcc tatcatgttg
     5941 atgcagttca ggctattggt aaaatcccaa ttcattcaga agaattgggc attgattttc
     6001 tcactgcttc tgcccacaaa ttccatggtc ctaagggaat cggttttctc tacgcatcta
     6061 gcatggactt tgattcctat ctacatggcg gagaccagga acagaaaaaa cgtgcaggaa
     6121 ctgaaaatct gcctgctatc gtaggcatgg ttgcagccct aaaagaagac ctagaaaaac
     6181 aagaagaaca ttttcaacat gtacaaaatc tagaaactgc ctttctggca gagctggagg
     6241 gcattcagta ttacttgaat agaggaaaac atcatctccc ttatgttctc aatattggat
     6301 ttcctggtca gaaaaatgac ctcttactcc ttcggctaga tttagctgga atttcaatct
     6361 ctactggctc agcctgtact gcaggcgttg tccaatccag ccatgttctt gaagccatgt
     6421 atggcgcaaa ttcagaacgc ttgaaggaat cccttcgcat cagtttgtcg ccacaaaata
     6481 ccgttgaaga cctacaaacc ctcgcaaaaa ccttaaaaga aattatcgga ggttagccat
     6541 atggcatttg aaaaaatcat tcagttaaaa aattgtcgtt acgattacac tcttagccct
     6601 tctgttaaaa aattcaccct caaagataac accttttttg aaactaaggt tggtaactat
     6661 gaactgactc gccttttgga aaaagtgcca aacagcggtg aaggcttcca actcaaaatc
     6721 atcattaaca aggaacttac aggggctaaa atcaatatca ctgacaagtt tggccttcgt
     6781 ctagttgata ttttcaaatc agaagaccac catattcatc aggaaaaatt ctacttcctc
     6841 atggatagct tggtagaacg tggtgtcttt acaaaatcgg aaagatagag ccaaaatgtt
     6901 tgaactgacc tataaggact gctatcatgt agagcgtact ctcaagtatg aggatcatga
     6961 ggctctcatg ttgactttgt caggatgtgt aaccttacca gatacacttt atgtgacttc
     7021 tctgactttt aggggccaaa aagtttatca gggtctggtc ggagacctct accgttttct
     7081 atctcatgca gattttttac atcaaaacta agatttt
//
LOCUS       DAVLFB010000095         6905 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLFB010000095 DAVLFB010000000
VERSION     DAVLFB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.095"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(102..377)
                     /locus_tag="VXD58_001771"
     CDS             complement(102..377)
                     /locus_tag="VXD58_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV0676426.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(484..1323)
                     /locus_tag="VXD58_001772"
     CDS             complement(484..1323)
                     /locus_tag="VXD58_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV0676427.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1465..2313
                     /locus_tag="VXD58_001773"
     CDS             1465..2313
                     /locus_tag="VXD58_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV0676428.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2406..3323)
                     /locus_tag="VXD58_001774"
     CDS             complement(2406..3323)
                     /locus_tag="VXD58_001774"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV0676429.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3760..3965
                     /locus_tag="VXD58_001775"
                     /pseudo
     CDS             3760..3965
                     /locus_tag="VXD58_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VXD58_001776"
     CDS             complement(4276..4569)
                     /gene="rpmA"
                     /locus_tag="VXD58_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV0676430.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4586..4930)
                     /locus_tag="VXD58_001777"
     CDS             complement(4586..4930)
                     /locus_tag="VXD58_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV0676431.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VXD58_001778"
     CDS             complement(4946..5260)
                     /gene="rplU"
                     /locus_tag="VXD58_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV0676432.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5296..5383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5442..6146)
                     /locus_tag="VXD58_001779"
     CDS             complement(5442..6146)
                     /locus_tag="VXD58_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV0676433.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6548..6847
                     /locus_tag="VXD58_001780"
     CDS             6548..6847
                     /locus_tag="VXD58_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV0676434.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2174 a         1404 c         1150 g         2177 t
ORIGIN      
        1 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
       61 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
      121 agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat ttctttacca
      181 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
      241 atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
      301 actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt agcgatcaaa
      361 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
      421 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
      481 tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat
      541 aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt
      601 catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagcacg
      661 ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc
      721 tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga tattgagaag
      781 agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat tttccaaagt
      841 tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc
      901 ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc
      961 ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg
     1021 gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc
     1081 accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt
     1141 tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc
     1201 cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa
     1261 ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt
     1321 catagtatca atctttctat tttaggattc agattggttt ccttacttct aattatatca
     1381 aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt tgatataatc
     1441 aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt taccatttta
     1501 ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca tcatctcttt
     1561 gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat ccctgtttcc
     1621 ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat ctttggagcc
     1681 aaatccctct attctagttt actaggaacc ttagctttgt ccgcctggtt agcttttttt
     1741 gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc ccttatagcg
     1801 ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac aactggcgga
     1861 actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg aaaactgctc
     1921 tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga tttgagattg
     1981 gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt gattggtgaa
     2041 ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca acttgctaag
     2101 gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg ctactatagt
     2161 aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt gaaaacgaag
     2221 gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc ccatgaaatc
     2281 ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg acctcaaaag
     2341 ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt tatttactca
     2401 agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc cactaattgg
     2461 tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac catgacggtt
     2521 tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc ttcttctcca
     2581 tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct ttgaatctca
     2641 acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag cactaaattc
     2701 gctgtcggat aaccaattgt acgaccacga gcattaccat gaaccaccat acctcttgat
     2761 ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa aatagcttga
     2821 cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac aatgataact
     2881 tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc aaatgtataa
     2941 tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc aaagaattct
     3001 tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc ttctacacct
     3061 tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa caaatctgga
     3121 tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac gataggcaat
     3181 aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc cttatgtatg
     3241 ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc tttttggttt
     3301 tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta tttctggaac
     3361 agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa aagcacttta
     3421 ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg taggacatct
     3481 tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc atactatgtt
     3541 atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat tggtgccaaa
     3601 aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa tgagacaact
     3661 tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc actttccctg
     3721 atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt ccaacaactc
     3781 ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt ggattctcag
     3841 accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa accattcaga
     3901 caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat agtcaattga
     3961 aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt gttctattcc
     4021 agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag gctgtacttg
     4081 agtacggcaa ggtgaaattg acgtggtttg aatttgattt tcgaagagta tcaatccact
     4141 atacatatta aaaagtatgg gtatcaaaat aaaaaaacag gctctccgaa aactcggaaa
     4201 gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc gggataaaag
     4261 gttcaatgaa cctttttatt ttgcgattgg gtaaacagaa acttgttttt tatcgcgtcc
     4321 tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt ctccaccacg
     4381 accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga ttgatccacc
     4441 tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg aatcacgtcc
     4501 gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt taagagtcat
     4561 ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt tggacgaact
     4621 cagaatagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag aataactggg
     4681 tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga tagccacctt
     4741 catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata gaattgataa
     4801 agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat tcgccactct
     4861 cggcgtgtcc agtaatttcc gcactcctca gctcgccatc ttcggctctc tcaaagactg
     4921 cctgtatcat gtgttctcct taaaattaag cgttgattgc gttgatgaca acttttgtat
     4981 atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac ttgtaagtaa
     5041 ccactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct ccagcaacaa
     5101 gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta aaagtaactt
     5161 cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc ttgaccaact tcaactttaa
     5221 cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc ctatgatttt
     5281 tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacga accacttaaa
     5341 cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata gtcaactctt
     5401 caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatga agctttttct
     5461 tttacttttt tctgccaaga ggcaaaaagc atgctgaggt aaaaaacgct catcataata
     5521 ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga aaagacaacg
     5581 ccaaggacaa aactactaag caggctaaca aatatgaatc cttcacgcaa aaaaggagtg
     5641 tgcttggttc ggaaataatc tccaaaagcc agcatggtcc gtttgatatt ccctgtcata
     5701 aaagcgttat tataggcaat acccgacact tctccaaaag cagttgtcac cagtcccata
     5761 cagaaggcca agggcggcac tagatagata ttatccacag tttgcggcac aaaagcaata
     5821 atgattgata agattgccaa gggaatcaag gacagaatag gttttttcac aattctcaat
     5881 ttttccttat aaatcgttaa taaaaagact cccatcataa acgctagcaa ggtgagaacc
     5941 ttgtccctaa catccgaaac attattttta attaattcta ctgaaagaaa gacaacattt
     6001 ccagtttgtc cagctacaag ggtattcccg cgaacaataa aagtgtaagc atccacatat
     6061 ccagcacaaa acgtcaaaaa aagtgctaac cttttagact gacgtgatat ttttcttata
     6121 ggtaataacc tcattttacc tcccattgta ttttctctta gaaatattgt accattttct
     6181 ttctaaaaaa tcgtaggcta ccatttagat tttactatta gcataaaaat aataatagac
     6241 aactatttat ccaaaaatag atagatgtaa catgtttgca aacaaagcat acgaaccttt
     6301 agtaaaatca tttccatgaa actagaatag agccctctta gcaaaaatca ttattttaat
     6361 ttatttctaa tcactccttg acataaataa ctctcaccaa taaaagacta tgtcttaaaa
     6421 aaatggtata ataaaatcaa tacttgggct tgatggctat gctactaata acaattagga
     6481 gagaaaatca ggcacttgtt aacaacaagg attatcccct tgagatgaaa ggaactttag
     6541 aaatcttatg atgaacatgc aaaacatgat gcgtcaagca caaaaacttc aaaaacaaat
     6601 ggaacaaagc caagctgaac ttgctgctat gcaatttgtt ggcaaatctg ctcaagatct
     6661 tgtccaagcg accttaactg gcgataagaa agttgtcagc attgatttca atccagctgt
     6721 cgttgaccca gaggaccttg agactctttc tgatatgacc gttcaagcca tcaactctgc
     6781 tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt gctttcgctg ggaaattacc
     6841 tttctaaaaa caaggagcta gaacaatgct tgtcgataac aaaggctaag aaaggtgcaa
     6901 aaatg
//
LOCUS       DAVLFB010000096         6746 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLFB010000096 DAVLFB010000000
VERSION     DAVLFB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.096"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..168
                     /locus_tag="VXD58_001781"
                     /pseudo
     CDS             1..168
                     /locus_tag="VXD58_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            335..535
                     /locus_tag="VXD58_001782"
     CDS             335..535
                     /locus_tag="VXD58_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676435.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            577..982
                     /locus_tag="VXD58_001783"
                     /pseudo
     CDS             577..982
                     /locus_tag="VXD58_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1920
                     /gene="ccdA2"
                     /locus_tag="VXD58_001784"
     CDS             1213..1920
                     /gene="ccdA2"
                     /locus_tag="VXD58_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV0676436.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1931..2488
                     /gene="sdbB"
                     /locus_tag="VXD58_001785"
     CDS             1931..2488
                     /gene="sdbB"
                     /locus_tag="VXD58_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV0676437.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2862..4253
                     /locus_tag="VXD58_001786"
     CDS             2862..4253
                     /locus_tag="VXD58_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV0676438.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4396..5313
                     /gene="adcAII"
                     /locus_tag="VXD58_001787"
     CDS             4396..5313
                     /gene="adcAII"
                     /locus_tag="VXD58_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV0676439.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5321..>6746
                     /gene="phtD"
                     /locus_tag="VXD58_001788"
     CDS             5321..>6746
                     /gene="phtD"
                     /locus_tag="VXD58_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV0676440.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPVQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVRDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFE"
BASE COUNT         2079 a         1243 c         1421 g         2003 t
ORIGIN      
        1 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgaa
       61 ttaatcgctc caatgactta cgaagagatg atgacgagcg acttttttga agtatggttt
      121 cagaagtttc tcttaccaac attaaccaca ccatcggtta ttatagtaaa atgaaataag
      181 aacaggacaa atcgatcagg acagtaaaat cgaattctaa caatgtttta gaagtccata
      241 tgtactattc tagtttcaat ataccataac tgttcctagt caacctgcaa gccctgtccc
      301 agctccaagc tcagctccga ctcaagatcc tagagtggta gcaagcgctt cggctagctc
      361 aacgagcaca caagctcaag agcaagttga caagtctgaa cttcgtgcct tgagtcaaga
      421 gttagaccaa cgcttgaaag ctttggcaac agtatctgat ccgaaaatcg atgcaaccaa
      481 ggctgtcctc ctagatgctc aaaaagctcc ggaagatagt gccttgacag agtaaggtct
      541 gcatactgca gtagagtctg tgaaggctgg gctgccttgg actctctcaa agatgtgaaa
      601 gcaaatgcta gcgacagcaa gcctacacag gacaagaagg atgcaaaaca aggaacggaa
      661 gatagtaagg attcagataa gatgactgaa acaaactcag ttccagcagg agtgattgtg
      721 gtcagtctac ttgccctcct aggcgtgatt gccttctggc taattcgccg taagaaagag
      781 tcagaaatcc agcaattaag cacggaattg accaaggttc taggacagct agatgcagaa
      841 aaaagcggat aaaaaagtcc ttgccaaagc ccaaaacctt ctccaagaaa cccttgattt
      901 cgtgaaagaa gaaaatggct cagcagagac agaaactaaa ctagtagagg agcttaaagc
      961 aatccttgac aaactcaagt aaaacatgat ttatgaaatg tcagacctaa aagacatttt
     1021 cgttcacaaa agcagcgacg agtaatcctt gtcgttgctt tttggctatt ttgggggata
     1081 agatccgcct tgactagcaa aagaaaagat tggtccaata gtgcccgata cgattttgcg
     1141 tagcagaaag cttacaaata aaattgaata tagtaaaata ggattagaat tattaagaaa
     1201 gttggttctt tattggaaac gatagtattt ttaatctctg tttttctagc aggtgtttta
     1261 tccttttttt ctccttgtat ttttcctctt ctgccagtct atgctgggat tttattggat
     1321 gatcaagaaa gtgcaaaaag cttttctttg tttgggagaa aggttctctg gtcaggcttg
     1381 attcgaacac tttgctttat cgctggtatc tctctcattt tctttattct aggctttggt
     1441 gctggttact ttggtcatat tctctatgca aattggtttc gatatggcat gggagctatt
     1501 attatcattt tgggtcttca ccagatggaa atttttcatt tgaagaaatt agaagttcaa
     1561 aaaagtttta cctttaaaaa atcagactct aatcgttatt ggtcagcttt tttacttggt
     1621 attaccttta gctttggttg gacaccttgt attggtccag ttttaagttc tgttttagca
     1681 cttgcggctt ctggaggcaa tggcgcttgg caaggagcga tttatactct catttacact
     1741 ctgggcatgg cccttccttt cttggtattg gcactagctt caggtctagt catgccatat
     1801 tttagtaaaa tcaagcgtca tatgatgcta ctaaagaaaa ttggtggttt cctcattgtt
     1861 ttaatgggaa ttttgttact attaggacaa gtaaatgttc tagctggaat ttttgaataa
     1921 aggaggacaa atgaaaaaag taatgtttgc tggcttaagt ctcttgtcat tagttgtatt
     1981 gatggcctgt ggtgaggaag aaactaaaaa gactcaagca gcacaacagc caaaacaaca
     2041 aacgactgta caacaaattg ctgttggaaa agatgctcca aacttcacat tgcaatccat
     2101 ggatggcaaa gaagttaagt tatctgattt taagggtaaa aaggtttact tgaagttttg
     2161 ggcttcatgg tgtggtccat gcaagaaaag tatgccagag ttgatggaac tagcggcgaa
     2221 accagatcgt gatttcgaaa ttcttactgt cattgcacca ggaattcaag gtgaaaaaat
     2281 tgttgagcaa ttcccacaat ggttccagga acaaggatat aaggatatcc cagttcttta
     2341 tgataccaaa gcaaccacct tccaagctta tcaaattcga agcattccta cagaatattt
     2401 aattgatagc caaggaaaga ttggaaagat tcaatttggt gctatcagta atgcggatgc
     2461 agaagcagca tttaaagaaa tgaactagaa tcgataaaat ctgattacaa gatgtagtca
     2521 gattttggta acttctaaaa ctaacttcca gtttcccaca acctagcttt tagtatgaga
     2581 aaaacgcgtg acatcagtgg aaatccgtct tagactaact tccactggtt ttctttttct
     2641 tgatatataa gggttcaaaa gcgaaaagac tcagaaaaag tgcacgacaa atagccctaa
     2701 aacagagtcg tcttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa
     2761 gttacggtag tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat
     2821 ttatggtaaa atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa
     2881 agaatgttag tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt
     2941 tgctgggtat cggagccatg gtagggacag gcgtctttac aatcacaggt actgcagctg
     3001 caacacttgc tggcccagcc ctagtgattt caatcgttat ttctgccttg tgtgtgggat
     3061 tatcagccct cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata
     3121 gttacctcta tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga
     3181 tggagttcat gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct
     3241 tgctctctca atacgggata gcctttcctc aggctttaaa tggtaccttt aatcctcaag
     3301 cagggacatt tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt
     3361 tactaaatgc caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg
     3421 ctttagctct ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt
     3481 ttgctcctta cggttttgga caaatctatg gagctagcac tggtattatg gctggtgcgt
     3541 ccttgatgtt cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca
     3601 agactcctca aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc
     3661 tctatgcctt ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg
     3721 atgatgccgt tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt
     3781 cattagtggc tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc
     3841 gtatgattta cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga
     3901 agactagcaa gataccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag
     3961 caggaatgtt cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact
     4021 tgatcatgtt ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg
     4081 gggaatttaa aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat
     4141 ccttcatgtt acagtataat atgaatacct ggattgcttt cctagttgcg ttgttagtag
     4201 gaagtattat ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa
     4261 gtctattgag ttgttgacac tcagcagatt tttatatgtg aaagaaattt caaatttttt
     4321 ctaaaaatat cttgacagat taaattttca ggagtagaat atttactagt taattaaatg
     4381 gttaaggagt tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt
     4441 ttgtgcttgg gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg
     4501 accagttttt atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt
     4561 cggatgattc agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca
     4621 gccatctatg atgcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga
     4681 agtctggatc caaatctaaa aaaatccaaa gtgaaggtct tagaggcttc tgagggaatg
     4741 accttggaac gtgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa
     4801 acgctctatg accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt
     4861 atcgctgata aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg
     4921 caagccttta tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa
     4981 gcgactcaga aaacatttgt aacacaacat acagcctttt cttatctagc gaagagattt
     5041 gggcttaatc aacttggtat tgcaggtatc tctcctgaac aagaaccaag tccacgacaa
     5101 ctaacagaaa ttcaggaatt tgttaagacc tataaggtta aaacgatttt tacagaaagt
     5161 aacgcttctt caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact
     5221 ctgaatcctt tagagtcaga cccacaaaat gacaagacct atttagaaaa tcttgaagaa
     5281 aatatgagta ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata
     5341 tctagcaggt tcagtggcag tccttgccct aagtgtttgt tcctatgaac ttggtcgtca
     5401 ccaagctggt caggttaaga aagagtctaa tcgagtttct tatatagatg gtgatcaggc
     5461 tggtcaaaag gcagaaaatt tgacaccaga tgaagtcagt aagagagagg ggatcaacgc
     5521 cgaacaaatt gttatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta
     5581 tcattactat aatggcaagg ttccttatga tgccatcatc agtgaagaac ttctcatgaa
     5641 agatccgaat tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggctatgt
     5701 gattaaggta gacggaaaat actatgttta ccttaaagat gcggcccatg cggacaatat
     5761 tcggacaaaa gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag
     5821 agcagataat gctgttgctg cagccagagc ccagggacgc tacaccacag atgatggtta
     5881 tatctttaat gcttctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg
     5941 cgaccattac cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga
     6001 agcctattgg aatgggaagc agggatctcg tccttcttca agttctagtt ataatgcaaa
     6061 tccagttcaa ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca
     6121 aaatcaaggg gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga
     6181 acgccatgtg gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc
     6241 cagaggtgta gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc
     6301 tgaattggaa aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt
     6361 acgagattca agaccagaac aaccaagtcc acaatcgact ccagaaccta gtccaagtcc
     6421 gcaacctgca ccaaatcctc aaccagctcc aagcaatcca attgatgaga aattggtcaa
     6481 agaagctgtt cgaaaagtag gcgatggtta tgtctttgag aagaatggag tttctcgtta
     6541 tatcccagcc aagaatcttt cagcagaaac agcagcaggc attgatagca aactggccaa
     6601 gcaggaaagt ttatctcata agctaggagc taagaaaact gacctcccat ctagtgatag
     6661 agaattttac aataaggctt atgacttact agcaagaatt caccaagatt tacttgataa
     6721 taaaggtcga caagttgatt ttgagg
//
LOCUS       DAVLFB010000097         6716 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLFB010000097 DAVLFB010000000
VERSION     DAVLFB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.097"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..271
                     /locus_tag="VXD58_001789"
     CDS             <1..271
                     /locus_tag="VXD58_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV0676441.1"
                     /translation="FFEAWFQKFLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKL
                     LPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            357..1307
                     /locus_tag="VXD58_001790"
     CDS             357..1307
                     /locus_tag="VXD58_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV0676442.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1484..2491
                     /gene="gap"
                     /locus_tag="VXD58_001791"
     CDS             1484..2491
                     /gene="gap"
                     /locus_tag="VXD58_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV0676443.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2559..3395)
                     /locus_tag="VXD58_001792"
     CDS             complement(2559..3395)
                     /locus_tag="VXD58_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV0676444.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3521..5716
                     /locus_tag="VXD58_001793"
     CDS             3521..5716
                     /locus_tag="VXD58_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV0676445.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLKNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYNSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5769..5921
                     /gene="rpmG"
                     /locus_tag="VXD58_001794"
     CDS             5769..5921
                     /gene="rpmG"
                     /locus_tag="VXD58_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV0676446.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5931..6107
                     /gene="secE"
                     /locus_tag="VXD58_001795"
     CDS             5931..6107
                     /gene="secE"
                     /locus_tag="VXD58_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV0676447.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6162..6698
                     /gene="nusG"
                     /locus_tag="VXD58_001796"
     CDS             6162..6698
                     /gene="nusG"
                     /locus_tag="VXD58_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV0676448.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2087 a         1176 c         1450 g         2003 t
ORIGIN      
        1 cttttttgaa gcttggtttc agaagtttct cttaccaaca ttaaccacac catcggttat
       61 tattatggat aatgcaagat tccatagaat gggtaagtta gaacttttat gcgaggagtt
      121 tgggcataaa cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac
      181 atgggctcat atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga
      241 ggctcttttg tcctgctctt gtttcaattg actatagttc acggatacag ttgggaaaga
      301 agctattaaa tatttggaga gtccttttgg gatgaacatg tcaaatatta ctatagatgg
      361 gcaagaaaag atgggcaaga aatggatctg agtcaaacga tgcttcttat gctcaagtag
      421 tgagtcttta tgatgataca agtatctctg ttagtaataa tgagacggac aaagtgttga
      481 ctggatccct ctatactgaa acaaatgaac agggattaac gattccaagc agtttactaa
      541 aaaattggaa tgaacagaca ggaaaaaatt tgacaactaa tgatcttatt ggcaaatcag
      601 tctcagccag cattgtagaa agtgctgccg aaactagtaa gattgctcaa tttcaaacga
      661 agattgtacg tgtaatcaat gatgaagatg acatggagga cagcaacagt ttcatgctgt
      721 ctcatcaaat ggaaacgatt ttgaaagagg ctggatttac gaaagctgta tcttatttta
      781 tcttggaact caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata
      841 agaagtatac tgtgctttct caacagagag ttcttgatat tgtgattacc tttattcgtg
      901 ttattcaggg attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga
      961 ttggtatcat tatttacatc aatatcatgc aacgttccaa ggaaataggt gtcatgaaag
     1021 cagttggtta tcagaatcgt ggtgtcaaag gaatttttat ttacgaggct atctggattg
     1081 taggcatcgc cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg
     1141 cgattgtaag tcacttttac ccatccatca ctaaggtttt tgaattaaat cttttatctg
     1201 ttttaggaac tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc
     1261 gtaagattag taaaatggat cctgtagaat cgttacgcta tgaataataa gggattcctt
     1321 gatttacagt cttaaaaaga aactcacata gaaaaacaat ggccatactt gcaattcaac
     1381 tcaaatagtt gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt
     1441 tcacaataaa aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg
     1501 tattaacggt ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg
     1561 tgttgaagtt acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa
     1621 atacgacaca actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggattcga
     1681 agttaacggt aaattcgtta aagtttctgc tgaacgtgat ccagagcaaa tcgactgggc
     1741 tactgacggt gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc
     1801 tgaaaaacac cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga
     1861 cgttaaaaca gttgtattca acactaacca cgacgttctt gacggtactg aaacagttat
     1921 ctcaggtgct tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagataa
     1981 ctttggtgtt gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat
     2041 ccttgacgga ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat
     2101 cgttcctaac tcaactggtg ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg
     2161 taaacttgat ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt
     2221 agcagttctt gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc
     2281 aaacgaatca tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc
     2341 ttacggttca ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt
     2401 ggttaaagtt gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac
     2461 tcttgaatac ttcgcaaaga ttgctaaata attcttgagt tgatagaaag caaggctttg
     2521 tggtcttgct tttttatatg gaaaaatgga tgacacgatc atccattctt ttttaattct
     2581 ttttcaaatg tatttgaaag ggtagtgaaa gttagcctct ctaaagtaag tgggtgggta
     2641 aaggaaagtc ggaaggcatg aagcataagc cggcttgtct ttgatttact attatagaga
     2701 gggtctccca ggataggaag attatgatgc aaaaggtgca cacgaatctg atgggttcgc
     2761 cctgtcttta gcttgcaatg agccaaggaa gtcttgtttg agaattgctt taatctgctt
     2821 acatgcgttt cagcatattt cccatttttt gcatcaacta ttctttttct acgatcatgg
     2881 cgatcacgtc caattttgtc tctgaaaaca agttcttttc tgttgatatt tccatcaact
     2941 agagcccaat attctctaga aatctctttt ttctccaata agcgattgag aatgggcagg
     3001 ataaaaggat ttttggcaaa gagaactaag ccactggttt ccatgtccag acgatgaacg
     3061 acatagcagg tttggccaac ataggtactg acatggttaa gaagggcaat ttcgtttggt
     3121 tgattaccat gcgttttcat cccctctggt ttgtttacaa taatcaagtg ttgatcttga
     3181 taaacttcct gcactaagtc tgggttgccc caagggatcg tcttttgggg ataatcttcc
     3241 tcgtcaaaag tcaactggca aacatctcca ggatttacga tttcgttcca gcggacttct
     3301 tcttgattta tcaaaatatg tttcttgatt ctcaaaaaat gacggatttt tctagggatg
     3361 aggagttgtt cctcaagtaa ttgctttacc gtcatttgag gtagagaggc gggtaatgta
     3421 aatgtgaatt gcatacagat attgtaacaa aaaaagccct atttggatag gaaatagcta
     3481 aattcttgtc ttcctatgat gaagatgata aaataaacgc atgaaattag ataaattatt
     3541 tgagaaattt ctttctcttt ttaaaaaaga aacaagtgaa ctagaggact ctgattctac
     3601 tatcttacgt cgctctcgta gtgatcgaaa aaaattagcc caagtaggtc cgattcgaaa
     3661 attctggcgt cgttatcatc taacaaagat tatccttata ctaggtttga gtgcaggctt
     3721 gctagttgga atctatttgt ttgctgtagc caagtcgacc aatgtcaatg atttgcaaaa
     3781 tgccttgaaa actcggactc ttatttttga ccgtgaagaa aaagaggctg gtgccttgtc
     3841 tggtcaaaag ggaacctatg ttgagctgac tgacatcagt aaaaacttgc agaatgctgt
     3901 tattgcgaca gaagaccgtt ctttctataa aaatgacggg attaactatg gccgtttctt
     3961 cttggctatt gtcactgctg gacgttcagg tggtggctct accattaccc aacagctggc
     4021 taaaaacgcc tatttatcgc aggatcaaac tgttgagaga aaagcgaaag aatttttcct
     4081 tgccttagaa ttaagcaaaa aatatagtaa ggagcaaatt ctaaccatgt accttaacaa
     4141 cgcttatttt ggaaatggtg tgtggggtgt agaagatgcg agtaagaaat actttggagt
     4201 ttctgcatca gaagtgagtc tggatcaagc tgcgactctg gcagggatgc tcaaggggcc
     4261 ggaactgtat aatcccttga attccgtaga agattctact aatcggcgcg atactgtctt
     4321 gaagaatatg gttgcagcag gatatattga taaaaaccaa gaaaccgaag ctgctgaagt
     4381 tgatatgact tcgcaattgc acgataagta tgaaggaaaa atctcagatt accgttaccc
     4441 ctcttatttt gatgcggtgg ttaatgaagc tgtttccaag tataatctaa cagaggaaga
     4501 gattgtcaat aatggctacc gcatttacac agagctggac caaaactacc aagcaaatat
     4561 gcagattgtt tatgaaaaca catcgctatt tccgagggca gaggatggaa cgtttgctca
     4621 atcaggaagt gtagctctcg aaccgaaaac agggggagtt cgtggagttg tcggtcaagt
     4681 tgctgacaat gataaaactg gattccggaa tttcaactat gcaacccaat caaagcgtag
     4741 tcctggttct acaattaagc ctttagttgt ttatacgcca gcagttgaag caggctgggc
     4801 tttgaataag cagttggata accataccat gcagtataac agctataagg ttgataacta
     4861 tgcagggatc aaaacgagtc gagaagttcc tatgtatcaa gccttggcag aatcgcttaa
     4921 tctacctgct gttgccactg ttaatgattt gggtgttgac aaggcttttg aggcaggcga
     4981 aaaattcgga ctcaacatgg aaaaggtcga ccgtgttctt ggtgtcgcct tgggaagcgg
     5041 tgttgaaacc aaccctcttc aaatggctca agcatacgct gcctttgcaa atgaaggttt
     5101 aatgcctgaa gctcatttta ttagtagaat tgaaaatgct agtggacaag ttattgcgag
     5161 tcataaaaat tcacaaaaac gggtgattga taagtctgta gctgacaaga tgaccagtat
     5221 gatgttgggg actttcacca acggtaccgg tattagttca tcgcctgcag actatattat
     5281 ggcagggaaa actggaacaa ctgaagcagt tttcaatccg gagtacacaa gtgaccagtg
     5341 ggtaattggt tatactccgg atgtagtgat tagccactgg cttggctttc ctaccactga
     5401 tgaaaatcac tatctagctg gctctacttc aaacggtgca gctcatgtct ttagaaacat
     5461 tgccaatacc attttacctt atacgccagg aagtaccttt acggttgaaa atgcttataa
     5521 gcaaaatgga attgcaccag ccaatacaaa aagacaagta caaaccaatg ataatagcca
     5581 gacagatgat aatttgtctg atattcgagg gcgtgcgcaa agtctagtag atgaggctag
     5641 ccgggctatc tcagatgcga agattaagga aaaggctcaa acaatatggg attcgatagt
     5701 caatctattt cgttaagatg cttgtcaaag cctagctttc ttgttataat ggataagatg
     5761 gaggcgttat ggcactaaaa aaagcaagcc tagcttgtgc ggtttgtggt tcgagaaact
     5821 attcaatcaa gatcagcgga aaccccaagc ctacacgact agaagtaaat aaattttgta
     5881 agcattgtgg caagtacact acacacagag aaacgagata ggagagagcg atgcgtttta
     5941 ttggagatat ttttagactt cttaaagaca caacatggcc aactcgcaag gaaagctgga
     6001 gagattttcg ttctatcatg gaatacacag ctttctttgt agtaattatt tacatttttg
     6061 accagttgat tgtttcaggt ttgattcgat ttattaacat tttttagaag attagtggag
     6121 ttaattacac tagaaatctt ctatttatga aaggaaatat catggatagt tttgataaag
     6181 gatggtttgt tttacaaact tattctggtt atgaaaataa ggtgaaagaa aatctattac
     6241 aacgtgcaca aacctacaat atgttggata atattctacg cgttgaaatt ccaacacaaa
     6301 cagtgcaagt tgaaaaaaat ggaaagagaa aagaagtaga agaaaatcgc tttccaggtt
     6361 atgttcttgt agaaatggtc atgacagatg aagcttggtt tgttgttcga aacacaccaa
     6421 atgttacagg atttgtcgga tcacacggga acagatcaaa accaactcca ttattggaac
     6481 aagaaattcg tgacattttg gtatctatgg gacaaactgt tcaagaattt gatttcgatg
     6541 ttgagattgg tcaaactgta cgtattattg atggtgcttt tgcagactac actggtaaga
     6601 ttacagaaat tgataataac aaagtgaaaa tgattatctc tatgtttggt aatgacacag
     6661 ttgcagaagt aaacctaaac caaatcgcag aattataacc ctgagagagg ctggag
//
LOCUS       DAVLFB010000098         6701 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLFB010000098 DAVLFB010000000
VERSION     DAVLFB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.098"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..2272
                     /locus_tag="VXD58_001797"
     CDS             <1..2272
                     /locus_tag="VXD58_001797"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV0676449.1"
                     /translation="RFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRFF
                     FTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDVF
                     RKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLATF
                     SNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKAS
                     DKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDYF
                     LPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVKA
                     DWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGGY
                     PGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYLL
                     NAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSIDF
                     RESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQA
                     IQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSST
                     NKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKGY
                     LNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNTD
                     YHLTVDLAQSMLTLPC"
     gene            2289..2759
                     /locus_tag="VXD58_001798"
     CDS             2289..2759
                     /locus_tag="VXD58_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV0676450.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3265..6615)
                     /locus_tag="VXD58_001799"
     CDS             complement(3265..6615)
                     /locus_tag="VXD58_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0676451.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
BASE COUNT         2083 a         1459 c         1134 g         2025 t
ORIGIN      
        1 gcgttttaat caatatagtt atatcaattt tccaaaagaa aatgtcttat ctgaactaaa
       61 aaaatgtggc tttgatttac aaaatacagc caatcacaaa gactcacttg aaaccttttt
      121 acgtcgtttc tttttcactt atcaagacac caactaccca ctttctatcc tagcagctga
      181 taaaaaaaca gacctgctga cattttttca atcagaagat gaactgacag cagatatttt
      241 ttatactgtt gcttttcaac ttttaggctt ttcttatttg gttgacttcg aagatagtga
      301 cgtttttcgt aaagaaactg gatttcccat tatatatggt gacttgattg aaaatctcta
      361 tcagttactc aatactcgca ccaaaaaagg aaatactctt atcgaccaac ttgttagtga
      421 tggtcttatt ccggaggata atgactacca ctactttaac ggcaagagtt tggctacttt
      481 ttctaaccag gatgtcattc gtgaagtcgt ttacgttgag tctcgtgtcg atactgacca
      541 aaaagggcta tcagacctag taaaggttag cattattcgc cctcgttttg atggaaaaat
      601 ccctgccatc atgacagcca gcccttatca tcagggaacc aatgacaagg ctagtgacaa
      661 ggctctctac aagatggagg gcgagcttga ggttaaactt cctcacaaga ttgagctaga
      721 gaaacctcaa ctaaatctcg tccaacccca aggtaaagct gagcttatag cagaggctga
      781 ggaaaagcta actcacatca actctagcta tacactaaac gactacttcc ttccgagagg
      841 ctttgccaat ctctatgtct caggtgttgg taccaaagac tcgacaggtt tcatgactaa
      901 tggagactac cagcaaatcg aggcttataa aaatgtcatc gattggctta acggccgttg
      961 ccgtgccttt actgatcaca cgcgccagcg tcaagtcaag gctgattggt caaacggaaa
     1021 agttgctaca acaggacttt cctatctagg taccatgtcc aatggtcttg cgactacagg
     1081 agtcgatggt ttagaagtta tcattgccga ggcaggaatt tcatcatggt acaactacta
     1141 ccgtgaaaac ggtctggtga ctagcccagg tggttatcca ggtgaggact ttgactccct
     1201 tgctgagtta acctattctc gtaatctctt agctggcgac tatatccgtg gcaatgaagc
     1261 tcaccaagct gacttagaaa aagtgaaagc tcagctagac cgaaaaactg gggactacaa
     1321 tcagttttgg catgaccgca attatctgct caatgcccat aaagtaaagg cagaggttgt
     1381 ctttactcat ggttctcagg attggaatgt caaaccactt catgtttacc agatgttcca
     1441 tgctcttcct actcatatac acaagcatct ctttttccat aatggtgccc atgtttacat
     1501 gaacaattgg caatcaattg acttccgtga gtccataaat gccttattga ccaagaaatt
     1561 actaggacag gaaacagact ttcaacttcc tactgttatc tggcaggaca atacagctcc
     1621 acagacttgg ttatcacttg ataacttcgg tgggcaagaa aactgtgaaa ccttctcact
     1681 tggtcaagaa gagcaagcta ttcaaaacca gtacccagat aaggattttg agcgctatgg
     1741 taagacatac caaaccttca atacagagct ctatcaaggg aaagccaatc agattactat
     1801 taaccttcct gtgactaaag atctccacct aaacggtcgc gctcagctca atcttcgtat
     1861 caaatccagt acaaacaagg ggctcttatc tgcccaactg ctagaatttg ggcaaaagaa
     1921 atacctacaa ccttatccag ctattttaag tgctagaacc attgacaacg gtcgctatca
     1981 catgttggaa aatctctgtg aattgccatt tagaccagag gcacaacgag tcgtgacaaa
     2041 aggttacctt aatttacaaa atagaaatga tttactgtta gtagaggata ttactgcaga
     2101 tgaatggatg gacgttcaat ttgaactgca gccaactatt tacaagctaa aagaaggaga
     2161 cactctccgt ttagtcctct atactactga ctttgaaatc accatccgtg acaatacaga
     2221 ctaccacttg actgtcgacc tcgctcagtc catgcttacc ttaccttgct aaaaggagtt
     2281 acattattat gaataaatca gaacaccgcc accaacttat acgcgctctt atcacaaaaa
     2341 acaagattca tacacaggct gagttgcaag cccttcttgc tgagaacgac attcaagtaa
     2401 cccaggcaac cctctcacgc gacatcaaaa atatgaacct atcaaaagtc cgcgaagaag
     2461 atagcgctta ttatgttctt aacaatggtt ccatctcaaa atgggaaaaa cgtctcgaac
     2521 tctacatgga agacgccctt gtctggatgc gcccagttca acaccaagtc ctactaaaaa
     2581 cccttcctgg actggctcaa tcctttggtt ctatcattga tactttgagc ttccctgacg
     2641 ctatcgctac cctttgtggt aatgatgtct gtcttatcat ctgtgaagat gcagatactg
     2701 ctcaaaagtg ctttgaagaa ctgaaaaaat tcgccccacc atttttcttt gaagaataat
     2761 aaatgactac aactaacaat tatgctttta aaaaacctgg tagaaagtct ttaacatcag
     2821 aaaacgcata gtatcaagtg ttgaaaaccc ttgatactat gcgttttgct ttatcctagg
     2881 aagatttata ctcttcgaaa atctcttcaa tccgcgtcag cttccatcta caacctcaaa
     2941 acagtgtttt gagcagcctg cagctagttt cctagtttgc tctttgattt tcattgagta
     3001 ttatttcatt ttctcctgca attgaattct tgctcggctt tttgttttct atttctttaa
     3061 aatcaaagta gctcttttgt taataactcg atcaacaaac atcgtggtac aagtatctac
     3121 tttgaaattt atcaaccact taacaactga tactgtattc taggaaaacg atgacattct
     3181 tcctaataaa acttctcata tatagcataa atttctactc tttttaattt taaatgagtc
     3241 tcagcatggc aatcacttta gaatttaagc cgcttctcca gctcgattat tgataagttc
     3301 aatgatttga acaatattac gaagtactgg tagttggtgt tggaagagta gttgaacaga
     3361 accataagat ttaaacggct cttgcgttaa cactttcgtc tctaaaaaac cattttctac
     3421 aatgtagtct acaatcaatt ttacaaatga aatctgcctg gcattaagat tctcatccga
     3481 caaaaattta gaaaaaatac gattggcaga ctctctatct aagccaataa tctcacgaac
     3541 caatctcgga attgccttat tttcataatg actttggtag tctgctttac tacctaattt
     3601 ttcccaaagc aatttttcaa gtgcaagcat atcatcagat gtcaactttt tattatggta
     3661 tagcttagaa atggactcct catccagatg agttttcaaa taatgctcaa ctttttcatt
     3721 gtaagaccga agatcgttga cctgcaaaaa tgctgtggtc tcgtgaacag tggagagtat
     3781 acgatcttca aagttaacat agtagggttt acggtctgtt ttatccaaaa actgtaataa
     3841 atctcgaata gcaagacgaa ttttttccaa atcagacaag ttaacttctt tccaaaattc
     3901 aggctcctgt actttcctga taatttcagc ctgctcaaaa acctgcggga tattgccaat
     3961 agcagaaaga gctctagccg tcttcatcac ttgggaaata tgaacagtgg aagatttagc
     4021 tgtcagttgc cccaactgaa tatgaagcaa ccacaaatca aatctcctcg ccatctcatc
     4081 ttctttatct tcatcaaata aaagcagaga gagatttttt tgaatggttt cacttgtaac
     4141 agcagttaga ttctgccaac tttccaattt cctatagcta taaactgtat ctaaaaccat
     4201 acgaaccctg aagtccaact catttaagct ctctatacga ccttgaagtt ccgagacaag
     4261 ctgctgacgg tatgctctcg caaactgatc ttcttggtat tggagtccct gaagttctcg
     4321 aatcaagtcc actttgatat taaataaacg ctgagtcaac gaaacaatgt gacgaccctc
     4381 tccatctctt ggatctgcac gaaaataatc aaaattgtcc ccataatcaa ataccaagaa
     4441 gttttcctta tcctgctcag gtccaaataa atctttacat agacgggttc ctcgaccaat
     4501 catctgccaa aacttagttt tagagcgtac tttcttgaag aagactaaat taacaacctc
     4561 tggtacatca atacctgtat ctaacatatc gacagaaatc gcaatttgag gatacttctc
     4621 cttaatttta aaatcatcaa tcaaggtctg ataatgctta atactataat caatcacctg
     4681 agcatagtcg ctcccttttt caggatagcg gttgttaaaa atacctctga tatattccgc
     4741 atgatcatga tttttagcaa aaataatagt tttaccaatt tcatcacccg aggctgtctg
     4801 aattcctctt gtcatgagtt catttaaaac aatttctact gtacttttat tgaaaacaaa
     4861 ggaattaaat acactcccat caatatcttt ttcacagcta tcgtcttcaa atttgctatc
     4921 aaaatgttcc ttttcttctt cggacaaatc atcataatgt agaccatccg taggtagttt
     4981 cagtttggtt tcgatagaat gataggctac taaatatccg tctttaacag cctcttccaa
     5041 atcatatgca tatgttggaa ccccattctc caaattaaag aatccatagg tgtttttatc
     5101 taaatcttga cgcggagtag ctgttaagcc tacaattctt gcatcaaaat aatcaaaaat
     5161 ggacttgtat ttctgataaa tagaacggtg agattcgtca attatgataa ggtcaaaatg
     5221 cccaacagtg aaagggcgtt gatttacttc ttcttgacca ctaatcgctc caatcatggt
     5281 cggataagtt gaaaagacca tgcgacttga ttgagctcct tctttatctt ctaagaagtt
     5341 acaaacggaa agatctggga gtaattttct aaacgaatca taggcttgct taaccaagga
     5401 agttctatcg gctaagaaga gaacgttttt tacccagtta tgacgtgata agatatcaac
     5461 tagagaaact gctgtacgag ttttcccagc cccagttgcc ataaccaaaa gtgcctgtct
     5521 acgatgatta gagaaagctt cacaaacgct tgcaatggca tgtttttggt agtgacgccc
     5581 ggaaatatcg tccctaattt tactagaaat atcctcaagt ggtttttgaa gatgacgtct
     5641 atccatcact aattgcaatt cttcttgaga gtaaaagcct gcaatctggc ggcggttcgg
     5701 accatcaagt atatagtgct tcaagccatt tgtaataaag caaattggct gatagccgat
     5761 atgtttctca agagcttcag catattcttt gacctgtact tcccctactt ctggattgac
     5821 agaggctttt ttagcctcta caatcgctaa aattttccca tttttaccat aaagtacata
     5881 gtcacagtaa ccaattcctg aaccgtgctt gagaccatca acggctatct caacacgaca
     5941 gttttcttca aatatccatc ctgctaaacg gagatcgata tcaataaaga gctttcttgt
     6001 ctctgcctca gataattgat caatatggaa aggcacatct tgccgttgcg ctgcagtctc
     6061 acgtttttca gtataagtct cttgaacttc tacggactga gcagccatct gttcatgaaa
     6121 atcaggcagt tctggtaaac tgtcttgtaa ctttatcata gattgtggag tttctcggta
     6181 tttgatgttt gctgaaagtg gtaagcactt ctcatcaaaa taacgctcca caaactcatt
     6241 gctgtaacaa taatcgataa aattaacaaa ctgatacaaa tgatgcaaag ctaaaatcgc
     6301 ttctcgttcc ttaatttcac caccatgagc agcatggttt ccccaccgaa tcaacagaac
     6361 tatctgcttg tggagttcag aatctacgat atccctaaaa tcatcatccc atactaaaga
     6421 agatagagta gcacgatagg gagcttctaa atatgaatcg tgactatata tccaatggac
     6481 agcctgctct aaagcacgac gcgccataaa ggcagtagcc acagttgatg tagcaatcat
     6541 attctcagct tcaatgcaag gttttgaaaa agattcatat tcgtcatttt ttaaaagaaa
     6601 gctaaaattt cccatttttt tctcacttta aacaaatagt ttagctatat tatactaaat
     6661 ttcaagctat ttctccacac ttttatagct atctcacaag c
//
LOCUS       DAVLFB010000099         6478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLFB010000099 DAVLFB010000000
VERSION     DAVLFB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.099"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            176..1459
                     /locus_tag="VXD58_001800"
     CDS             176..1459
                     /locus_tag="VXD58_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV0676452.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(1654..3225)
                     /gene="ffh"
                     /locus_tag="VXD58_001801"
     CDS             complement(1654..3225)
                     /gene="ffh"
                     /locus_tag="VXD58_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV0676453.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(3237..3569)
                     /locus_tag="VXD58_001802"
     CDS             complement(3237..3569)
                     /locus_tag="VXD58_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV0676454.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(3660..4037)
                     /locus_tag="VXD58_001803"
     CDS             complement(3660..4037)
                     /locus_tag="VXD58_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV0676455.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            4109..5413
                     /locus_tag="VXD58_001804"
     CDS             4109..5413
                     /locus_tag="VXD58_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV0676456.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            5426..6232
                     /gene="yidA"
                     /locus_tag="VXD58_001805"
     CDS             5426..6232
                     /gene="yidA"
                     /locus_tag="VXD58_001805"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV0676457.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
BASE COUNT         1833 a         1505 c         1197 g         1943 t
ORIGIN      
        1 catttttttc tgctatacta tcctaagcaa aggtttttaa tgtcatcccg tgaggtgacg
       61 aagacgcaga aatatttgaa actctttaaa gtctagattt tagagaagtc ttactctgag
      121 ggcctattgc tgtaaaataa tgggctcttt tctgatgccc aaaagtgagg tttatatgaa
      181 acaagaatca actgttgatt tgttactaga cgttgaccaa cgtccttcgg atggaaaagg
      241 aattctcctt agcttccaac acgttttcgc catgtttggt gcgaccatct tggtaccatt
      301 gattttggga atgcctgtat ctgttgccct ttttgcttca ggtgttggaa cactcatcta
      361 catgattgct actggtttta aagttccagt ttatctaggt tcttcatttg cctttatcac
      421 agctatgtca ctggctatga aagaaatggg gggggatgta tctgctgccc aaacaggggt
      481 tatcttgact ggtttggtct atgtccttgt tgctaccagc atccgatttg taggaacaaa
      541 atggattgat aaactcttgc caccaatcat tatcggtcct atgatcatcg ttatcggtct
      601 tggacttgca ggttcagctg ttaccaatgc aggtcttgta gcagacggaa attggaaaaa
      661 tgctctggta gccgttgtta ctttcctaat tgctgccttt atcaatacaa aaggaaaagg
      721 cttcctacga atcattccat tcctctttgc cattatcggt ggttaccttt tcgcactaac
      781 tcttggcttg gttgacttta caccagttct taaagccaac tggttcgaaa ttcctggttt
      841 ctacttgcca tttagcacag gtggtgcctt taaagagtac aatctttact ttggtccaga
      901 agccatcgct atcttgccaa tcgctatcgt aacaatttct gaacatatcg gagaccatac
      961 tgttttgggt caaatctgtg gccgtcaatt cttaaaagaa ccaggtcttc accgtactct
     1021 tattggtgac ggtatcgcaa cttctgtttc tgccttcctt ggtggaccag ccaatacaac
     1081 ttacggagaa aatacagggg ttatcggtat gactcgtatc gcttctgtct cagttatccg
     1141 taacgctgcc ttcatcgcga ttgccctcag cttccttggt aaattcactg ccttgatttc
     1201 aactattcca aacgctgtac ttggtggtat gtcaatcctt ctctatgggg ttatcgccag
     1261 caatggtttg aaagtcttga ttaaagaacg tgttgatttc gctcaaatgc gaaacctcat
     1321 catcgcaagt gctatgttgg ttcttggact tggaggagct atccttaaac ttggtccagt
     1381 tacactttca ggtactgccc tttcagccat gacaggaatc atcttgaact tgatcttgcc
     1441 atacgaaaat aaagactaag agtctaaata cacctaatcc actcagacag ctgagtggat
     1501 ttttcgtata tcataataaa agtgtcttaa caaaattatt aaaatcaaaa aacgtataat
     1561 atcagatatt ctaaaacctt gatactgtac gttttatcat agaaattttt actttatttt
     1621 ctcatcaaat gagatttgca tcaatctctt gtcttacttg cgtttcttct tcgctttctt
     1681 cattttgtta gccatacgtt tcatggactg tttcatggca aattcaccaa ttttaccttt
     1741 caaaccgcca ccaaacatct ggctcatatc tggcattcct gctcctccga gagctgataa
     1801 gtcaggcata ccgccttgtc ccatcattcc ttcaagggca gacatatcca ttcctcccat
     1861 atttggcata tttttaggaa ggttatttgg attaatcccc atttgcttca tcattttatt
     1921 catatcccca gacataacac cctgcatgag ctgtttagcc tggttaaagt ccttgataaa
     1981 tttattgact tcgacgaatg tatttccaga accagcagca atacgacggc gacggcttgg
     2041 atttaacaaa tctgggtttt cacgttcttc aggtgtcatc gaagacacaa tggcacgttt
     2101 acgagcaatc tggcgttcat ccaccttcat gttttgaagt gctggattgt tggccatacc
     2161 tggaatcatc ttgagcaagt cttccatcgg ccccatattt tgcacctgat ctaattgatc
     2221 gatgaaatca ttaaaatcaa aggtgttttc gcgcatcttc tcagccattt caagggcttt
     2281 ttgttcatcg tattcctgag aagctttctc aatcaaagtg agcatatccc ccatgccaag
     2341 gatacggcta gacatacggt ctgggtggaa ggtttcgata tctgtaattt tttcacctgt
     2401 accagtgaac ttgattggtt ttccagtgat gtgacgaacc gatagagccg caccaccacg
     2461 agtatcgcca tcaatcttgg taaggatgac cccagtcact tccaactgag cattaaactc
     2521 acgcgcaaca ttggctgctt cctgaccaat catggcatca acgacgagga gaatttcatt
     2581 tggttgagcc aatgctttca catcacgaag ctcattcatg aggagctcat caatttgcaa
     2641 acgacccgca gtatcaatca agacatagtc gttatgatta gtttgggctt gctccaaacc
     2701 ttgacgtaca atctcaacag ctggtacttc tgttccaagt gcaaagacag gcacatcaat
     2761 ctgttgtccc aaagtcttaa gctggtcaat ggcagctgga cgataaatat ccgccgcaat
     2821 catcaaagga cgagcatttt cttctttctt gagtttgttg gccaatttac cagcaaaggt
     2881 tgttttacca gccccttgta aaccaaccat catgatgatg gttggaatct taggtgactt
     2941 gataatttct gccgtatcag aacctaaaac ggctgtcagt tcctcatcaa cgattttaat
     3001 aatctgttgc gcaggattaa gtgtatcaat gacctcatgc ccgactgcac gctcacgaac
     3061 tttcttgata aagtccttta caacaggcaa agcaacgtcg gcctcaagca aggccaagcg
     3121 aatttctttg gttgcctctt ggacatcaga ttcagagatt tttccttttt tacgtagact
     3181 tttaaagacg ttctgcaaac gttctgttaa actttcaaat gccatttttc ttcctcttat
     3241 tctctattat caatgcttgt taaaatttct atctgctcct gcagaaagtt atccttggga
     3301 tagcgctcca aaatctgatc aaaaatctga ctgcggacaa tatagtccga gtacatgtgc
     3361 aatttcatct cataatcttc cagaatcttt tctgttcgct tgatattgtc atagacagcc
     3421 tgacgactga caccgaactc ctcggcaatt tcagcaaggc tgtaatcatc agcgtagtag
     3481 agctcgatat aattcatttg cttatctgtc aaaagcgccg cataaaattc aaagagcgca
     3541 ttcatacgat tggttttttc gatttccata acttttatta taccaaaaat tagcctaatc
     3601 taccacacta ggaagccgat ccaagaagat agatagctaa atttgaaaaa gacataagcc
     3661 tagccccaag taatttccaa ttgatagctg gcaaagggat gtccctcttg attttgtagt
     3721 tgataatcta gttcaatctt ttgcccatcc acttgataac gattcgtttg gataataaac
     3781 tcctgcatac ccataggtgt gggaatatag gctaaactat cactatcctt tagaaagcgc
     3841 ataatggtct tgggattgga aaatcgactc atcacaagtt cttgaccatg aaatttaatc
     3901 actacttttt ccttttcctc attatagaaa agcaggtagc tataatctcc tttttcatgc
     3961 acttccacat cataaagctg gtcaatcact tccaactgct catcaaactg aattgtattt
     4021 cgcatccgaa tcttcacatc aggccctctt tcttgtctct tgtcctacta ttttaccaaa
     4081 aagagcagga ttttgctata atggtcatat gaacgaaaaa gtattccgtg accctgttca
     4141 caactacatc catgtcaata atcaaatcat ctatgacttg attaatacaa aagaatttca
     4201 gcgtttgcgc cggatcaaac aactgggaac ttccagttat accttccacg gtggagaaca
     4261 cagtcgcttc tctcactgtc taggagtcta tgaaattgca cgacgcatca cagagatttt
     4321 cgaagaaaaa tatcctgagg aatggaatcc tgccgagtct ctcttgacca tgaccgctgc
     4381 tctcctacac gaccttgggc atggtgccta ctcccatact tttgaaaatc tctttgatac
     4441 agaccatgaa gccattactc aggagattat tcaaaatcct gagacagaga ttcaccaagt
     4501 cctgctacaa gtggcacctg atttcccaga aaaggtggcc agtgtcattg accataccta
     4561 tcctaataag caggtcgtgc agctcatttc tagtcagatt gacgcagatc gcatggacta
     4621 tctcttgcgc gactcctatt ttacaggagc atcctatggg gaatttgacc tgactcgaat
     4681 cctccgagtc attcgtccta tcgaaaatgg tatcgccttt cagcgcaatg gcatgcacgc
     4741 catcgaagac tacgtcctca gtcgctacca gatgtacatg caggtttatt tccaccccgc
     4801 aacacgcgcc atggaagttc tcctacagaa tcttctcaaa cgcgccaagg aactctatcc
     4861 tgaggacaag gatttctttg cccgaacttc tccacacctc ctgcctttct tcgaaaaaaa
     4921 tgtgaccttg actgactatc tggctctgga tgatggcgtg atgaatacct acttccagct
     4981 ttggatgacc agtcctgaca agattcttgc agatttatcg catcgctttg tcaaccgcaa
     5041 ggtctttaaa tccattacct tttcacaaga agaccaagac caactcgcta gcatgagaaa
     5101 attggttgag gacatcggct ttgatcccga ctactatact gccattcata agaactttga
     5161 cctcccttat gatatttacc gtcccgaatc tgaaaatcca aggacacaga ttgagatttt
     5221 acaaaaaaat ggagaactgg cagaactctc tagcctgtcc cctatcgtcc aatcccttgc
     5281 tggaagtcgc cacggagata atcgctttta tttcccgaaa gaaatgttag accaaaacag
     5341 catcttcgca agcatcaccc agcaattttt acacttgatt gagaacgatc attttacccc
     5401 aaataaaaac taaaagagga aatttatgag tattaaacta atcgccgtcg atattgacgg
     5461 aaccctagtc aacagccaaa aggaaatcac tcctgaagtc ttttctgcca tccaagatgc
     5521 caaagaagct ggtgtcaaag tcgtgattgc aactggccgc cccatcgcag gtgttgccaa
     5581 acttctggac gacttgcagt tgagagacca aggtgactat gtggtgacct tcaatggtgc
     5641 ccttgtccaa gaaactgcta ctggccatga gattatcagc gaatccttga cctatgagga
     5701 ttatctggat atggaattcc tcagtcgcaa gctcggtgtc cacatgcatg ccattaccaa
     5761 ggacggtatc tatactgcaa atcgcaatat cggaacatac actgtacacg aatcaaccct
     5821 cgtcagcatg cctatcttct accgtacccc tgaagaaatg gctggcaagg aaattgtcaa
     5881 gtgtatgttc atcgatgaac cagaaattct cgatgctgcg attgaaaaaa taccagccga
     5941 attttacgag cactactcta tcaacaaatc tgctcctttc tacctcgaac tccttaaaaa
     6001 gaatgtagac aagggttcag ctattactca cttggctgaa aaactcggat tgaccaaaga
     6061 tgaaaccatg gcaatcggtg acgaagaaaa tgaccgtgcc atgctggaag tcgttggaaa
     6121 ccccgttgtc atggaaaatg gaaatccaga aatcaaaaaa atcgccaaat acatcactaa
     6181 aacaaatgac gaatccggcg ttgcccacgc catccgaaca tgggtactgt aaaagtatca
     6241 tttttcaata agaattgatt agcaataaaa tccaatgaat ttttttagca aactatttaa
     6301 tttaaaacaa aataatcata atagagacac aaattctgat tgtaacaatt tttacctaaa
     6361 cgatttagaa tgtggcctta ctcctgggca actcatactc atagattgga ctcaaaaaac
     6421 agggagaaat tataatttcc caagatattt taaatactct cttcaaattg accctgaa
//
LOCUS       DAVLFB010000100         6186 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLFB010000100 DAVLFB010000000
VERSION     DAVLFB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.100"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            28..1407
                     /gene="glmU"
                     /locus_tag="VXD58_001806"
     CDS             28..1407
                     /gene="glmU"
                     /locus_tag="VXD58_001806"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV0676458.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1417..1962
                     /locus_tag="VXD58_001807"
     CDS             1417..1962
                     /locus_tag="VXD58_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0676459.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            1974..2288
                     /locus_tag="VXD58_001808"
     CDS             1974..2288
                     /locus_tag="VXD58_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV0676460.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2305..2997
                     /locus_tag="VXD58_001809"
     CDS             2305..2997
                     /locus_tag="VXD58_001809"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV0676461.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            3074..3565
                     /gene="rocS"
                     /locus_tag="VXD58_001810"
     CDS             3074..3565
                     /gene="rocS"
                     /locus_tag="VXD58_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV0676462.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3663..4253
                     /locus_tag="VXD58_001811"
     CDS             3663..4253
                     /locus_tag="VXD58_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV0676463.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4262..6025
                     /locus_tag="VXD58_001812"
     CDS             4262..6025
                     /locus_tag="VXD58_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV0676464.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         1899 a         1050 c         1356 g         1881 t
ORIGIN      
        1 gggaatttct acagaaaaga gaagattatg tcaaattttg ccattatttt agcagcgggt
       61 aaagggactc gcatgaaatc tgatttgcca aaagttttgc acaaggttgc gggtatttct
      121 atgttggaac atgttttccg tagtgtggga gctatccaac ctgaaaagac agtaacagtt
      181 gtaggacaca aggcagaatt ggttgaggag gtcttggctg gacagacaga atttgtgact
      241 caatctgaac agttgggaac tggtcatgca gttatgatga cagaacctat cttagaaggt
      301 ttgtcaggac acaccttggt cattgcagga gatactcctt taatcactgg tgaaagcttg
      361 aaaaacttga ttgatttcca tatcaatcat aaaaatgtgg ccactatctt gactgctgaa
      421 acggataatc cttttggcta tggacgaatt gttcgtaatg acaatgctga ggttcttcgt
      481 attgttgagc agaaggatgc tacagatttt gaaaagcaaa tcaaggaaat caacactgga
      541 acatacgtct ttgacaacga gcgtttgttt gaggctttga aaaatatcaa taccaataac
      601 gctcaaggcg aatactatat tacagacgtc attggtattt tccgtgaaac tggtgaaaaa
      661 gttggcgctt atactttgaa agattttgat gaaagtcttg gggtaaatga ccgtgtggcg
      721 cttgcgacag ctgagtcagt tatgcgtcgt cgcatcaatc ataaacatat ggtcaacggt
      781 gttagctttg tcaatccaga agcaacttat atcgatattg atgttgagat tgctccggaa
      841 gttcaaatcg aagccaatgt taccttgaaa gggcaaacga aaattggtgc tgagactgtt
      901 ttgacaaacg gtacttatgt agtggacagc actatcggag caggagcggt cattaccaat
      961 tctatgattg aggaaagtag tgttgcagac ggtgtgacag tcggtcctta tgctcacatt
     1021 cgtccaaatt caagtctggg tgcccaagtt catattggta actttgttga ggtgaaagga
     1081 tcttcaatcg gtgagaatac caaggctggt catttgactt atatcggaaa ctgtgaagtg
     1141 ggaagcaacg ttaatttcgg tgctggaact attacagtca attatgacgg caaaaacaaa
     1201 tacaagacag tcattggaga caatgtcttt gttggttcaa attcaaccat tattgcacca
     1261 gtagaacttg gtgacaattc cctcgttggt gctggttcaa ctattactaa agacgtgcca
     1321 gcagatgcta ttgctattgg tcgcggtcgt cagatcaata aagacgaata tgcaacacgt
     1381 cttcctcatc atcctaagaa ccagtaggag cctatcatgg agtttgaaga aaaaacgctt
     1441 agccgaaaag aaatctatca aggaccaata tttaaactgg tccaagatca ggttgaatta
     1501 ccagaaggca agggaactgc ccaacgggat ttgattttcc acaatggggc tgtctgtgtt
     1561 ttagcagtaa cggatgaaca aaaactgatc ttggtcaagc agtaccgcaa agctatcgag
     1621 gctgtctctt acgaaattcc agccggaaaa ttggaagtag gagaaaacac agcccctgtg
     1681 gcagctgccc ttcgtgaatt agaggaagaa acagcctata cagggaaatt agaactcttg
     1741 tacgattttt attcagctat tggcttttgt aatgagaagt taaaactata tttagcaagc
     1801 gatttgacaa aagtggaaaa tccgcgtccg caggatgagg atgaaacctt ggaagtcctt
     1861 gaagtgagct tagaagaagc gaaagaatta atccaatcag gtcatatttg tgatgccaag
     1921 acaattatgg ctgttcagta ttgggagttg cagaaaaaat agaggaggtc agtatgggta
     1981 aatctttatt aacggatgaa atgattgaaa gagctaatag aggcgaaaaa atttcaggtc
     2041 ctcctttgct agatgataat gaggagacta agattttacc aacctcttct tcccgttttg
     2101 gttatgccaa tcctaagaat catggtttta gccaggaaac cttgaagatt caggtcgaac
     2161 catctattca taaaagccgt cgcattgaaa ataccaagag aaatgtcttc aattctaagt
     2221 tgaataaaat cttattcgcg gtcatctttc tcttgatttt gcttgtttta gcaatgaaac
     2281 ttttgtaata gaaaaggaat tgaaatgaaa ataggaatta ttgctgctat gccagaagaa
     2341 ctggcttatc tggtccagca tttatataat gtccaggagc aagttgtttt ggggaatacc
     2401 tatcatacag gaaccattgc ttctcatgaa gtcgttcttg tagaaagtgg aattggtaag
     2461 gtcatgtctg ctatgagtgt ggcgattttg gctgatcatt tccaggtgga tgcccttatt
     2521 aatacgggtt cagctggggc agtagcagaa ggtatcgctg ttggggatgt cgtgattgct
     2581 gacaaattag cctatcatga cgtggatgtc acagcttttg gctatgctta tggacaaatg
     2641 gcgcaacaac cgctttattt cgaatcagac aaaacctttg ttgctcaaat ccaagagagt
     2701 ttatctcaat tggaccaaaa ctggcatctt ggtttgattg ctacaggaga tagttttgtt
     2761 gcaggaaatg acaagataga agcgattaag tcccatttcc cagaagtttt agccgtggag
     2821 atggaggggg cagctattgc tcaagcagcg cataccctca atctcccagt cttagtcatc
     2881 cgagctatga gtgacaatgc caaccatgaa gcaaacatct tttttgatga gtttattatc
     2941 gaagctggac gtcgctctgc ccaagtcttg ttggcctttt tgaaggcttt agattaagcg
     3001 gaaatttgac agtttttcta gcttatgata agatttaagt aaagaaaagc tagaaaacgt
     3061 ttcagaggat attatgagta ttgaaatgac cgtcagtgag attgcagagg tcttaggatt
     3121 atctcgccaa gcaatcaata accgtgtcaa agaattacca gaagaagaca cagataaaaa
     3181 tgacaaaggg gtaacagttg ttaccagaag tggcttgatt aagctagaag aaatctataa
     3241 aaaaacgatt tttgaagatg aacctgtcag tgaagatgtc aaacaacgtg aactgatgga
     3301 gattctagtg gatgagaaga atgcagaaat tcttcgtctt tatgaacaat taaaagccaa
     3361 ggatcgtcag ttatcagaaa aagacgagca gatgcgtatc aaagaccgtc agattgctga
     3421 gaaagacaaa caattagatc agcaacaaca attgaccctt caagccatga aggatcaaga
     3481 aaatcttaag ctagagctgg accaagcaaa agaagaagtc caatccacta aaaaaggctt
     3541 ttttgctcgt ttatttggag gataatcaag gagctgttta ggttaaatgc taacctgatg
     3601 gcttcttttg tttgtgaata gtatagttga ttagaacaga gacagtagag gagaggtaca
     3661 gaatggaacg attagaagat tacattgctt ttgatttaga atttaatcag cacgaaggac
     3721 aaacacactt gattcaagta tcggcagtgc gttttagaga tggtcgggaa atcgatgcct
     3781 atgattccta tgttcatacc agcgtccctc taaaaagctt tatcaatggt ttgaccggga
     3841 ttacagctga gaccttaaaa gatgctcctc cagttgagaa agtcttagaa gaattccaag
     3901 agtttgtggg ttctttgctg atgataggct acaatgctgc taaaagtgat ttacctattt
     3961 tagcagagca tggtttagac tatagccaac agtacaaggt ggatttgtat gatgaagctt
     4021 ttgaacgtcg gagttcagat ttacatggta ttgccaatct caaattgcaa actgttgcct
     4081 catttcttgg atttcaaggt cagtctcata atagtttaga ggatgctcgg atgacggcgc
     4141 gtgtttacga atccttctta gagtcggatc aagctaggga attgttagag acagaaagta
     4201 gcttatcaaa caatcctttt ggtggcttgg acttgtctca attatttgac taggagtgcc
     4261 tatgaaacct gaaaaaccta aaaaattggg tcttaaacag atttacctca atcttgataa
     4321 aatcctcttt ctatttttct tgatttttat gatagtggag tatttgtggg taccattcaa
     4381 ttcatggttg gctagcttct tattagatca gacaggttat ctttttattt cctataacaa
     4441 tgtcttagcc atccttactc attctccatt gattagtcta gcttttttag ccttgattgt
     4501 aattaacttt ctggttgctt attttcaaat aggactcctc tttattggtg ctcgtcatct
     4561 cctctataat gaaaagcgaa ggctaattga gtatatccgc aaggttttcc atgaaagttt
     4621 tgaatttatg aaaaagctaa ctggcgctaa agccttgttt atctttttct atatagctat
     4681 gctgtttcct tttatacgga aaatgctgaa gatttattat cttaataaaa tcgtcattcc
     4741 agagtttatc caagcttatt tagaagataa atattggatg tggtggctga gcatcctact
     4801 tctatctctt atctttctct atgtctctgt cagattgatg tttgcccttc cgaagattgt
     4861 atatgatcag ctgactgttc gagaagcagt gatgtttagc ttggagaaaa ccaagaagag
     4921 agttactttt tatgcttgga atttatttta catcttgctc aaagcacatt tattatttta
     4981 tcttcctttg attcctttat tattggctca aactttggta gatgatatca ctcagagaga
     5041 gtctctgatt cttggtattt tgaattttgt cgtgattaaa aatctctatt atatggctct
     5101 gacttatttt ttggttaaat ttgtttcatt tttgacagga aaggagctgg atatgcttcc
     5161 taggagagaa aaagatcaca tcgtgcgatg gggtgtcatg acttgtgcca gttttttctt
     5221 tgccttagaa ggttatattt atctggaggc tcccatggtt catctacctc aacttatttc
     5281 tcaccgaggg gtttccaatg caaatgggat tcaaaataca gtagagtcct tggaaactac
     5341 agcacaactc aaaccagact tggtggagac ggacgtgcag gaaacaaaag atgggcagtt
     5401 tgtcatgatg catgatgcta acttgaaaaa tctagcaggt atcaataaaa gtcctcaaga
     5461 cttaaacttg gaggagctta aagggattga tatttttgaa aatggctacc agactaaaat
     5521 ttcaagcttt gaagattatc tcagtcgagc caacgaactt ggtcaaaaat tactaattga
     5581 aattaaaacc agtaaaaaag atagtccaga catgatgaac cgctttttag cccgttatgc
     5641 tgcaaagctc aagatttatg gacatcaaat ccagtcttta gactaccatg ttgtcgaaaa
     5701 agtaagacag tatgatgccg aactgccagt ttatttcatc atgccctaca attctgtctt
     5761 tcctaaaaca agagcgacag gatatactat ggagtactca accttggatg aatattttgt
     5821 aagcaaacta tggacaacgg atcagaaact ttatgtctgg actgtcaatg attcagaagc
     5881 tatcagcaaa tctcttcact taggagtaga tggagtgatt acagatgatt tggaaaaagt
     5941 tcagcgggaa atagaagtag cccaagaaga tccagaatat acggatttgc tcttgaaaaa
     6001 agcattggaa ttctttgaat tttagtaata aaaaagagga acggttgttc ctcccatgat
     6061 aggtaagtat tcttgtgaat gcttgctttt ttgtggttgc aatccgttta cgagaattgt
     6121 ttgtattgtt cttgtttcaa tttactttat agtgctttga agttggaatg ctacactata
     6181 acttct
//
LOCUS       DAVLFB010000101         6077 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLFB010000101 DAVLFB010000000
VERSION     DAVLFB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.101"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            201..2534
                     /locus_tag="VXD58_001813"
     CDS             201..2534
                     /locus_tag="VXD58_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0676465.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRTALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAMVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            2547..4010
                     /locus_tag="VXD58_001814"
     CDS             2547..4010
                     /locus_tag="VXD58_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0676466.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            4010..5578
                     /locus_tag="VXD58_001815"
     CDS             4010..5578
                     /locus_tag="VXD58_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676467.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMP
                     ISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIF
                     PYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIIS
                     KVDLLFQKVSQLFE"
     gene            complement(5526..6071)
                     /locus_tag="VXD58_001816"
     CDS             complement(5526..6071)
                     /locus_tag="VXD58_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676468.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT         2163 a          910 c         1176 g         1828 t
ORIGIN      
        1 tgataataag tcgcctgccg aaaacagcga aaaatagcag tgttatgcgg agataatctg
       61 acgcgatgcg aaagtatatt gcatacttat tttcaacaat ttagcagagt atttttataa
      121 gtgtgatata atagaagtat aatttgttct gatagtttat tttatggaga agtagatttt
      181 tagaatgcgg aggattcaat atggttgagt ttataaagtc taagaaagaa atgagtgagg
      241 aggatattaa agcaaatttc atcactcctg ctattgtatc caaaggatgg aaaaatggtg
      301 agcatatcgc ttacgaagaa tacttcactg atggtcgaat tgaagttaga ggagataagg
      361 ctcgtcgtaa agaaggaaaa aaatcagact attcactgta ttaccaattt ggaactcgaa
      421 ttgcaattgt tgaggcaaag gataataaac acagcgttcg agcaggatta caacaagcta
      481 ttgaatatgg agagatttta gatgttccat ttgtttattc ttcgaatggt gatggcttta
      541 ttgaacacga ccgtatcacg agagaagaac gtgagctgga gttagacgaa ttccctactc
      601 gtgaagaatt attttctcgt atgacgaagg aaaaaggatt gacgtacgaa attacagaag
      661 ctatctcaac tccatactat acagacgcct tctcaatgaa aacgccacgc tattatcagc
      721 aaatagctat caaccgtact attgaaacag ttgccagagg acaaaaacga gtaatgtttg
      781 tgatggcaac aggaacgggg aaaacgttca tggcttttca aattattcat cgccttcgaa
      841 aagctggttt ggctaaacga gttttattct tagcggatag aaacatctta gtagaccaaa
      901 cgatggctga agactttaag ccattcgaaa aggtaatgac gaaaattaca ccaaaacttt
      961 tgactgctcc tgaaaaatta aattcttttg aaatttatct agggctttat cagcaactaa
     1021 ctggtgaaga tggaactgaa acacattatc aaaaatttga taaagacttc tttgatttaa
     1081 tcgtaattga tgaagcgcac cgtggttcag ctaaggaaaa cagtaactgg cgtaagataa
     1141 ttgattattt cagttctgcg acacagattg ggatgaccgc tactcctaaa gaaaccaaga
     1201 atgcttccaa tacggaatac tttggtgagc caatctatac ttatagttta aaacagggaa
     1261 tcgaggatgg ttttttggct ccatatcgtg ttatgagggt taatttagat gtggatgtgg
     1321 atggttatcg tccagaaact ggaaaagttg atactaacgg acaattaata gaagataggt
     1381 actacggcag gaaagatttt gataaaacca ttgtcattga tgatagaacg caaagagttg
     1441 ccaagtttgt ttctgattat atgaagcaaa acaatgcacg atttgataaa acaattgttt
     1501 tttgtgttga tattgaccat gccgagcgaa tgcgtactgc acttgtaaaa gagaatctag
     1561 acttagtcca agaagactat cgttatgtca tgcaagtaac tggtgacaac gctgaaggaa
     1621 aagctcaact ggataacttt atggatgtca attctaattt tcccgctatg gtaacaacgt
     1681 ctaaattatt aacgacagga gttaatgcta aaacatgtcg tttgattgtt ttagactcta
     1741 atatccaatc catgactgaa tttaaacaaa ttattggtcg tggcacacgt ctttatcctc
     1801 aaaaggggaa agaatttttt acgattattg attttcgaaa tgttaccaat ttgtttgctg
     1861 accctgattt tgatggtgag ccagtgaagg tgctggaaac aggtgcgaaa acagtcagtg
     1921 gttccacgcc cggtttcgta gatgaggaag gtgacccagt agaaaaatat atcgttacag
     1981 acaagcaggt taccattctt aattctactg ttcaagtatt ggatgaaaac gggaaactga
     2041 ttaccgaaag cctgaccgac tacactcgga agaatatctt aggtagctac gccactttga
     2101 acgattttat cacagtttgg catacggcgg ataaaaagaa gcttatctta gacgaacttt
     2161 ataaaaaagg agtttatcta gatgctattc gagagtcgga gggaatatca gaacaagaaa
     2221 tcgatgattt tgatttactc ctaaaacttg cctatggtca aaaagaatta accaaaacgg
     2281 aacgtatcaa taagctcaaa caaagcggat atttatataa atatagtgag gaagcgcgtg
     2341 ctgttttgga aattttactg aacaaataca tggataaagg tattggagaa ctcgaaagca
     2401 ttgaaacatt aaaacttcca gaatttcaga tatatggtgg aaccttcaaa atcatcaata
     2461 cttattttgg agataaaaaa cgatatttac aagcaattaa agaattggag caagagctat
     2521 ttacagtagc ttaatgaaag gaaagtatgt caattacatc atttgtaaaa agaattcaag
     2581 atatcactcg aaacgatgct ggtgttaatg gtgatgctca acgtattgag caaatgtctt
     2641 ggttattatt cttaaaaatt tatgatagcc gtgaaatggt ttgggaatta gaagaagacg
     2701 agtatgagtc aattatccca gaggaattaa aatggcgaaa ttgggctcat gctcaaaatg
     2761 gggaacgggt attgacaggc gatgaattac ttgattttgt caataacaag ttattcaaag
     2821 agttgaaaga gcttgaaata acttcaatta tgcctattcg aaaaacgatt gttaaatcag
     2881 cttttgaaga tgcgaacaac tatatgaaaa atggcgtctt gttacgccaa gtcatcaatg
     2941 ttattgatga agttgatttc aatagccctg aagatcgtca ttcgtttaat gatatttacg
     3001 aaaaaattct taaagatatt caaaatgctg ggaactcagg agaattttat acgccacgtg
     3061 cagcgactga ttttattgcc gaagttctta acccaaaact tggagaatca atggcagacc
     3121 ttgcttgcgg aacaggaggc ttcttgactt cgactctgaa ccgtttaagt agtcaacgta
     3181 aaactagtga agataccaaa aaatataata cagctgtttt tggtattgaa aagaaagcat
     3241 ttcctcatct tttagcagtt acaaatctgt ttcttcacga aattgatgac cctaaaattg
     3301 ttcatggaaa tactttggag aaaaatgttc gtgaatatac ggatgatgaa aaatttgaca
     3361 ttattatgat gaatccacct tttggagggt cagaattaga aacaataaaa aataactttc
     3421 cagcagaatt acggagttct gaaacagctg atttatttat ggctgtcatt atgtatcgtt
     3481 tgaaagaaaa tggtcgtgtt ggagttattt tacctgatgg ttttctattt ggtgaaggtg
     3541 taaaaactcg cttgaaacaa aaactggtag atgagttcaa cttgcatacg attattaggt
     3601 tgcctcatag tgtctttgca ccgtatacag gaatccatac gaacattctt ttctttgata
     3661 aaacaaagaa aacagaagaa acttggtttt atcgtttaga tatgccagat ggttataaaa
     3721 atttctcgaa aactaagccg atgaagtcag aacacttcaa tcctgttcgt gactggtggg
     3781 aaaatcgtga agagattctg gaaggtaagt tctacaaatc taaatcattt acacctagtg
     3841 aattggctga gttgaattat aatttagacc agtgtggctt tccaaaagag gaagaggaaa
     3901 tcttaaatcc ctttgagttg attcagaatt atcaagcgga aagagcaact ttaaatcata
     3961 agattgataa tgtattagct gatattttgc agttgttgga ggacaaataa tgacaccaga
     4021 acaacttaaa gcaagtattc tccaaagagc gatggaaggg aaattagtgc cgcaaaatcc
     4081 caatgacgaa cctgcaagtg aattattaaa gagaattaaa gctgaaaaag aaaaacttat
     4141 cagtgaagga aaaatcaaac gagataaaaa ggaaactgag atatttcgtg gtgatgatgg
     4201 gaaacattat gggaagtttg ctgatggaag cactcaagaa attgatgttc cttatgatat
     4261 tcctgatact tgggagtggg tgaggttttc tacattggtt gaaattgtca gaggtggctc
     4321 tccacgacca atcaaagatt atcttacttc tgaagtagat ggaataaatt ggataaaaat
     4381 aggtgatact gaaaagggtg aaaagtatat aaataatgtt aaagaaaaaa tcaaaaaatc
     4441 agggcttaac aaaactagat ttgtaaaaaa aggtacattt ttgttaacta attctatgag
     4501 ttttggtaga ccttatattt tgaatgttga tggtgcaata cacgatggat ggttggctat
     4561 ttcgaactat gaaaactcat taaataaaga ttacctattc tatattcttt catcaaatgt
     4621 agtttattct caatttctat ctctaattag tggagctgtt gtgaaaaact tgaatagtga
     4681 taaagttgct tctattctta tccctctccc cccactatcc gaacaacaac gcataataga
     4741 agcaatcgaa tcagctttag aaaaagtaga tgaatatgct gaaagttata atagactaga
     4801 acagctagat aaagaatttc cagataaact aaaaaaatct attcttcaat atgctatgca
     4861 aggaaaatta gttgaacaag acccaaatga tgaatcagtc gaagttttac ttgaaaaaat
     4921 acgagcagaa aaacaaaaac tctttgaaga aggcaagatt aaaaagaaag atttggacat
     4981 ttctattgtt tcccaaggag atgataactc ttattatggg aacaaagacg aaacaacctc
     5041 ttatcctata tatgaaatcc cagaagcatg gagatatatt aaatttgcta gcctagttaa
     5101 cttcagaata ggaaaaactc ctccacgcag tgaagctact ttttggggaa ctgaaatacc
     5161 ttgggtatct atatcagata tgcctatatc tggttatgta actaatacga gagaatctat
     5221 ttctaaatta gcattaaaat caaagaaaat agacatttca cctaaaggaa ctttattaat
     5281 gagttttaaa ttatctattg gaaaagttgc catattggat attcctgcca ctcataatga
     5341 agccattata tcgatattcc cctatgctaa taaagaaaat attatcagag attacttaat
     5401 gatatttttg ccacttatct ctactttagg tgattcaaaa gatgctataa aagggaaaac
     5461 attaaatagt accagtatct ccgaattatt aatccctatt tctaatcatg aagagatgaa
     5521 aagaatcatt tccaaagttg atttactttt tcaaaaagtt tctcaacttt ttgagtaata
     5581 agttcctgtt cctcaaaagg agctaacgga attaatagct cgcttagtgt agttttagga
     5641 atattatata aagcttgacc tgatagttta gttattgctt tcaattgttt ataaaataac
     5701 ggagaggaca agttaaataa tagaaatttt gaaataatct ctgaactttc gaatggtgtt
     5761 aattggaaaa taaatccacc agccacaaca ccatcatagt ctttatcgat tcttgcaaac
     5821 tttccaatat gttctaaaga ggttgataca ggtgttatta gctgattatg ttttaaataa
     5881 acttgttcag aggagatgaa ttgtgtatca atgtagtaat cattatccaa cagagaaaat
     5941 tctaaaggct taatattacc accacgtata attctaacac ctttattatt aatgcttaaa
     6001 tcgcccttct tgtaagaaag acctgtattc attgaaaaaa tatcttttat ttttataaca
     6061 acccaattca taggtat
//
LOCUS       DAVLFB010000102         5843 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLFB010000102 DAVLFB010000000
VERSION     DAVLFB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.102"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            2..676
                     /locus_tag="VXD58_001817"
     CDS             2..676
                     /locus_tag="VXD58_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010019642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV0676469.1"
                     /translation="MKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAGAKGIR
                     ANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNR
                     SRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYTPYSPK
                     KDNPDFELAERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPKEIAQR
                     FINVIK"
     gene            701..1618
                     /locus_tag="VXD58_001818"
     CDS             701..1618
                     /locus_tag="VXD58_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV0676470.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            1646..3187
                     /locus_tag="VXD58_001819"
     CDS             1646..3187
                     /locus_tag="VXD58_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV0676471.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAIAIVYSYTGGLKSVLWTDFIQGVVLISGVVVALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLAIATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGVTGL
                     ILAAIYAAAQSTVSTGLNSVATSWTLDIQDVISKNMSEDRRTKIAQFVSLAVGLFSIV
                     VSIIMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTENKVSAPKYTTIHDISEIKADSS
                     WEVRH"
     gene            3213..3671
                     /locus_tag="VXD58_001820"
     CDS             3213..3671
                     /locus_tag="VXD58_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV0676472.1"
                     /translation="MIISEQSDFRRYASVNKHFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            3673..>5843
                     /locus_tag="VXD58_001821"
     CDS             3673..>5843
                     /locus_tag="VXD58_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV0676473.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTSISEVQHKSGNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVTRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPAPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIREHGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTQVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQISIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHM"
BASE COUNT         1912 a          894 c         1155 g         1882 t
ORIGIN      
        1 aattaaaaat ttaaaaggtc aaataattgt ttcctgccaa gcgttagaag gagagccact
       61 ttatactcca aatggaggag tgatgccgtt attagcaaaa gccgcctttc aagcaggcgc
      121 taaaggtatt cgtgctaatt ctgtccgcga tatttcagaa ataaaagaag aagttgactt
      181 accaattata ggtatcataa aaagagatta cgatggcttt gaacctttta tttcagcaac
      241 tatgaaagag attgatgaat tagttagtga aggagtagac attttagctt tggattgtac
      301 taatcgttca agaccaggtt atgataatat tactgacttt attcatgata ttaaagtcaa
      361 gtatccaaac caattattaa tggctgatat ttctactttt gaagaaggga aagtagctgc
      421 tgaatcggga gtagattttg tgggtacgac attatctggt tatacaccat atagccctaa
      481 aaaagataat cctgattttg agttagctga aagattggta aaagagcttg acgtaccagt
      541 tattgctgaa ggaagaataa gtactccgga acaagctaga aaaatgttag atttaggtgc
      601 ttacgcagtg gttgttggag gagctatcac tcgtccaaaa gaaattgctc agcgatttat
      661 taatgtcatc aaataaaaaa caatataaag gagaaatctg atgaaagatt taactaaata
      721 caaaggcgtt attcctgcat tttatgcttg ctatgatgaa aatggtgaaa ttagccaaga
      781 acgtgtaaaa tctctggtgc aatatttcat tgacaaaggt gtaaaaggta tctatgtaaa
      841 tggttcttca ggtgaatgta tttaccaaag tgtagaagat cgtaaacaaa ttattgaagc
      901 tgttatggaa gttgctaaag gtaaattaac agttatcaac catgttgcat gtaataacac
      961 gaaagatagt attgaattgg caaaacattc agaaagtgtt ggagtcgatg ctattgctgc
     1021 tatcccacct atttacttca aattgccaga atattcaatt gcagcatatt ggaatgcaat
     1081 gagtgaagct gcgccaaata cagattttat catttataat attccacaat tggcaggggt
     1141 tgcgttgact ggtagtttgt atgcaacaat gcgtcaaaat cctcgtgtga ttggagttaa
     1201 aaattcttct atgcctgtac aagatattca aatgtttgta gctgcaggtg gagaagatta
     1261 cattgtattc aatggtccag atgaacaata tcttggtggt cgcttgatgg gagcagaagc
     1321 tggtattggt ggtacttatg gcgttatgcc agatttgttc ttgaaattgg aaagtttgat
     1381 tcaagaacga gatttagata ctgctaaaaa acttcaatat gctatcaatg aagttatcta
     1441 taagatgata tcaggtaagg caaatatgta tgctgtagca aaagaggttt tgcgtctaaa
     1501 tgaaaaactt gatttaggtt ctgttcgtca acccttagaa gcattggcag aaggtgactt
     1561 ggaagttgca aaacaagcag cagaacttat tcaacaagca cgaaaagaat ttttataata
     1621 aataaaatca atatggagat atgttatggg cacaacagga tttacaataa ttgacttaat
     1681 tatcttgatt gtttatttac ttgcggtgtt ggttgcaggt atctatttct ctaaaaaaga
     1741 gatgaaagga aaagagttct ttaaaggaga tggttcggtt ccttggtatg ttacttcggt
     1801 atccattttt gccacaatgc tcagtccgat ttccttcttg ggactcgctg gtaactctta
     1861 tgcaggtagc tggattttat ggtttgctca attagggatg gttgtagcta ttccactgac
     1921 aattcgtttt atcttaccta tctttgcacg gatagacatc gatacggcat atgattactt
     1981 ggataaacgt tttaattcta aagcacttcg tattatttca gcactcttgt ttattattta
     2041 tcaattggga cgtatgtcta tcattatgta cctcccatca gctggtttat cagtattgac
     2101 aggaattgac atcaatattt tgattatttt gatgggtgca attgcaattg tttattctta
     2161 tactggtggt ctaaaatccg tattatggac agactttatt caaggtgtgg ttctgattag
     2221 tggtgtcgtt gtagctttat ttgtactgat ggctaatatt aaaggtggct ttggtgcagt
     2281 agcagaaaca ttagcaaacg ggaaattcct tgctgcaaat gaaaaacttt tcgatcctaa
     2341 cttgctttca aactccatct tcttaattgt gatgggttca ggctttacaa tcttgtcttc
     2401 ctatgcttca tctcaagatt tggttcaacg ttttactaca acacaaaata ttaagaaact
     2461 taataaaatg ttgttcacaa atggtgtctt gtcacttgca attgcaacag tcttttactt
     2521 gatcggtaca ggcttgtacg tattctatca agtacaaaat gcaggtagtg cagctagcaa
     2581 tatccctcaa gaccaaatct ttatgtactt tattgcatac cagttaccag taggtgtcac
     2641 aggtttgatc ttggcagcga tttatgcagc agctcaatca actgtttcaa caggtttgaa
     2701 ctctgttgca acttcatgga cattggatat tcaagatgtc atttctaaaa atatgtcaga
     2761 ggatcgtcgt acgaaaattg cacaattcgt atctctagca gtaggtttat tctcaattgt
     2821 tgtttccatt atcatggctc attcagatat taaatctgca tacgaatggt tcaatagttt
     2881 catgggactt gtacttggtc tacttggtgg tgtattcatt cttggttttg tttctaaaaa
     2941 agcaaataaa caaggtgctt atgcagcgct gattgtatca accatcgtta tggtatttat
     3001 taaatacttc cttcctccaa cagctgttag ctactgggca tattcattga tttcaatttc
     3061 tgtatcagta gtttcaggtt atattgtatc tgttcttact gaaaataaag tatctgcacc
     3121 taaatataca acgattcatg atatttcaga aattaaagcg gattcaagtt gggaagttcg
     3181 tcactagtca ctaatagaaa agagaaaata atatgattat ttctgaacaa tctgatttta
     3241 gacgatatgc ttctgtcaat aaacattttt caaaagtctg tgatttttta gaaaatacaa
     3301 atttgacaga tttagttgat ggaaaaattg atattgacgg ggaaaatgtt tttgcaaatt
     3361 gtatgactta tctagctgat ggagttccag gagatatttt tgaaactcat aagaaatatt
     3421 tagatattca tattgttgtt gaaaatacag aaaaaatggc agtaacttcc ccagttcgtg
     3481 ctcagtcacg tgtacctttt agcgaagaga aggatattgc tttttacgat agcaaagact
     3541 atcaaattgt tgaattgctt ccagggaata tgttggtgac ttttgaagaa gatcttcacc
     3601 aaccaaagat tcattgcaat gatgaaactg ttaaaaaact tgttatcaaa gttttaaacg
     3661 aggaaaaata aaatgaaaaa aaatattaaa caatatgtaa ccttaggtac tgtagtggta
     3721 ttatcagcat ttgttgctaa ctcagttgca gctcaggaga ctgaaacttc tgaagtatca
     3781 acaccagagt tggtgcaacc tgttgcacca acgacttcga tttcggaagt acaacataaa
     3841 tcgggtaact cttcggaagt tactgtacaa cctcgaacag ttgaaactac tgttaaggat
     3901 ccatcttcta cagcggaaga aactcctgtc ttagaaaaaa ataatgttac tttaacaggg
     3961 ggcggagaaa atgttactaa tgagttaaag gataaattta ctagcggtga ctttactgta
     4021 gtgattaagt acaatcagtc tagtgagaaa ggtttacaag ctctgtttgg aatatctaat
     4081 tctaaacccg gtcaacaaaa tagttatgta gatgtgttcc ttagagacaa tggtgaattg
     4141 ggaatggaag cgcgtgatac ttcttccaat aaaaatcacc tagtaaccag acctgcttca
     4201 gtttggggta agtacaaaca agaggccgtg acgaacactg ttgcagtagt agcagattca
     4261 gtcaaaaaaa cgtattcttt atatgcaaat ggtacaaaag tagtagaaaa gaaagtggat
     4321 aacttcctaa acatcaagga tattaaaggt attgattact atatgcttgg gggagtgaaa
     4381 cgtgcaggaa aaacggcgtt tggttttaac ggaacactag aaaatatcaa attctttaat
     4441 agtgcattgg atgaagaaac tgttaaaaag atgacaacaa acgctgttac tggacattta
     4501 atttatacgg ctaatgatac aacaggttct aactatttcc gtattccagt tctgtatact
     4561 tttagcaatg gtcgggtttt ttcaagcatt gacgctcgtt acggtggaac tcatgatttc
     4621 ttgaataaaa ttaatattgc tacaagttat agtgatgata atggtaagac atggactaaa
     4681 ccaaaattaa cattagcatt cgatgatttt gcgccagcac cattagaatg gcctcgtgat
     4741 gttggtggac gtgacttaca aatcagcggt ggtgcaacct atattgactc tgttattgtt
     4801 gaaaaaaata acaaacaagt actcatgttt gctgatgtta tgcctgctgg agtaagtttt
     4861 agagaagcta ctagaaaaga ttcaggttat aaacaaattg atggtaatta ttaccttaaa
     4921 ttaaagaaac aaggtgatac tgattataat tatactattc gtgagcatgg tactgtatac
     4981 gacgatcgta ccaacagacc aactgaattt tcagtagata aaaatttcgg tattaaacaa
     5041 aatggtaatt atttgacggt agagcaatat tcggtttcat ttgaaaataa taaaaagact
     5101 gaatatcgta atgggactca agtccatatg aatatcttct acaaagatgc attattcaaa
     5161 gtagtgccaa cgaactatat tgcatatatt tctagcaatg atcatggcga atcttggtct
     5221 gcaccaactt tattacctcc tataatggga cttaatcgga atgcacctta tttgggtcct
     5281 ggacgtggaa tcattgaaag ctcaactgga cgtattctta ttccgtctta cactggtaaa
     5341 gagtctgcat tcatttatag tgacgataat ggagcgtctt ggaaagttaa agttgtgcca
     5401 cttccttcta gttggtcagc agaagcacaa tttgtagaat tgagtccagg agtaattcaa
     5461 gcatacatgc gtacaaataa tggtaaaatt gcatatttaa caagtaaaga tgcaggtact
     5521 acttggagtg cgccggaata tttgaaattt gtttcaaatc caagttatgg aacacaatta
     5581 tcaatcatca attatagtca attgattgat ggtaaaaagg ctgtcatttt aagtactcca
     5641 aactccacaa atggtcgtaa acacggacaa atttcgattg gtctaattaa tgatgataat
     5701 acaattgatt ggcgttatca tcacgatgtt gattatagta actatggata ctcatattca
     5761 acattgacag agttaccaaa tcatgaaatt ggattgatgt ttgaaaaatt tgattcatgg
     5821 tctcgtaatg aacttcatat gaa
//
LOCUS       DAVLFB010000103         5698 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLFB010000103 DAVLFB010000000
VERSION     DAVLFB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.103"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..209
                     /locus_tag="VXD58_001822"
     CDS             <1..209
                     /locus_tag="VXD58_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV0676474.1"
                     /translation="NFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVRYGIGCHRDFI
                     VYHYRVAYRLYLEKLVMNRGFISC"
     gene            440..1726
                     /locus_tag="VXD58_001823"
     CDS             440..1726
                     /locus_tag="VXD58_001823"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV0676475.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(1968..3116)
                     /locus_tag="VXD58_001824"
     CDS             complement(1968..3116)
                     /locus_tag="VXD58_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV0676476.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKPKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKQKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(3289..4101)
                     /locus_tag="VXD58_001825"
     CDS             complement(3289..4101)
                     /locus_tag="VXD58_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV0676477.1"
                     /translation="MQKTVEKILFRVAGVTKYKKAVKEACNMIAEDNGIPEYSKYYGD
                     LSAKEIREEVEEYGLKVFKYRDLDIFNIELIPETDNKYDPNAIKVLIFDNHIGYVPAT
                     VSKSIRKYFDDERYHFLIECEIKGGPYKEWDDYEEKVVINNDLDVGFEIYLTIVDSSQ
                     KEVIQSESSEIIDDNIFNKEVTKTESIETKTTEAEHIEQNIVSDSFADIVNEINLSET
                     SPKKKLPANKIIFSALYLFLVFFGVVGIPIAPFLAVPLTAWSLYKLYKLFRK"
     gene            complement(4139..4522)
                     /locus_tag="VXD58_001826"
     CDS             complement(4139..4522)
                     /locus_tag="VXD58_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV0676478.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPTEFDYLK
                     FMSYYNLKTVTNEVMVKEEYLALVN"
     gene            complement(4535..4900)
                     /locus_tag="VXD58_001827"
     CDS             complement(4535..4900)
                     /locus_tag="VXD58_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676479.1"
                     /translation="MFSLFEKIKELCQNRGISINSLEETLGYSRNTIYSMKNKKPNAE
                     RLQEIADYFNVSTDYLLGRTDNPAIAGDSKEYTWQGKTLNVEEMASNVMMFGGRELTD
                     EKKKIIQSIIEGYLKEAGD"
     gene            complement(5206..5688)
                     /locus_tag="VXD58_001828"
     CDS             complement(5206..5688)
                     /locus_tag="VXD58_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676480.1"
                     /translation="MKSSFFHYLKEIILYGVLVPILTVIVGFHLFTTFYPISDSLLTD
                     FLKAFTEQRILAIVFVLILCFIFFGSIYYCISCGRNRKLIPDKVYTQITTYISSVGLG
                     TAVIAVATLTLTELQFSILTGWIAFIALLLPLLKFKVQYSTYQSSDNVAQKTTENKAN
                     "
BASE COUNT         1628 a          943 c         1118 g         2009 t
ORIGIN      
        1 ctaatttcta tgatagtatt gaagaagagt atggtcctac atttggtgat aattttgact
       61 gggaacatgt tcattttaaa tttttaattt attatttagt gagatatggc attggttgtc
      121 atagggattt tatcgtttac cattatcgtg ttgcttatcg tttgtatctt gaaaaattgg
      181 taatgaatcg gggttttatt tcttgttgag gtaattttag taaatttccg aactaattta
      241 ctcttttatg taaagataat agtaaatagc tagtaatttt tctaaatcat tttttaatag
      301 ttggaaatag caaatctttc tattgtttct tcttgataaa aaggcgattt tttattataa
      361 taaattgtaa gatataattg caggtgagag tcctgccatg tatgtgagaa aggaagagcc
      421 tgagggctca gacaaaatta tgacttcagt tgttgttgta ggtacccaat ggggtgatga
      481 aggtaaaggg aagattacag acttcctttc agcgaatgca gaagtgattg cacgttacca
      541 aggtggtgat aatgctggtc acacgattgt gattgacggt aagaaattta agttgcactt
      601 gattccatct gggattttct tccctgaaaa aatatctgtc attgggaatg gtatggttgt
      661 aaatcctaaa tctcttgtaa aagagttgag ctatcttcat gaggaaggtg taacaactga
      721 taacttgcgt atttctgatc gtgcgcatgt tattttgcct tatcatatcg agttggatcg
      781 cttgcaagaa gaagctaagg gcgacaataa gattggtacg acaattaagg gaattggtcc
      841 agcttatatg gacaaggctg ctcgtgttgg aattcgtatt gcagatcttt tagataaaga
      901 tattttccgt gagcgtttag aacgtaacct tgctgaaaag aatcgtcttt ttgaaaaatt
      961 gtatgacagt aaagcgattg ttttcgatga tatttttgaa gaatattacg aatatggtca
     1021 acaaatcaag aaatacgtga tagatacatc tgttatcttg aatgatgcgc ttgataatgg
     1081 caaacgtgtg ctttttgaag gtgcacaagg tgttatgcta gatatcgacc aaggtactta
     1141 tccatttgtt acgtcatcaa accctgtagc tggtggtgtg acaattggtt ctggtgtcgg
     1201 tccaagcaag attgacaagg ttgtaggtgt atgtaaagct tatacgagtc gtgtaggaga
     1261 tggtcctttc ccaactgagt tgtttgatga agtgggagaa cgtatccgtg aagtgggtca
     1321 tgagtatggt acaacaactg gtcgtccacg tcgtgtaggt tggtttgact cagttgtgat
     1381 gcgtcatagc cgtcgtgttt ctggtattac taacctttct ttgaactcta ttgatgtttt
     1441 gagcggtttg gatactgtga aaatctgtgt ggcctatgat cttgacggtc aacgtattga
     1501 ctactatcca gctagtcttg agcaattgaa acgttgcaag cctatctatg aagagttgcc
     1561 aggttggtca gaagatatta ccggagttcg caatttggaa gatcttcctg agaatgcgcg
     1621 taactatgtt cgtcgtgtga gtgaattggt tggcgttcgt atttctactt tctcagtagg
     1681 tcctggtcgt gaacaaacaa atattttaga aagtgtttgg tcctaagaga tttttaagat
     1741 ttgtttaaga taggtcgggt atactataga cagttacaag aagacctcct aacttgttgt
     1801 aacaaatatc ctaaactttt ctttttcata ataatctccc ttaactccac ccaatcaggt
     1861 ggagtttttt agctttattt caggctttgg gggactattc taaaaataat ttttcgatat
     1921 ttttcggtat ttttcggatt ttggtcgggg aattggcggg gactttttta gcgaatatga
     1981 ctaagaaata ggtctgttgt cgcttcagca agttcgtcct ctacttgatt gtaacgatcg
     2041 gtcatataga cttttgtatg gcccagcgcc tggcttaatt gttcaagcgg aacccctgca
     2101 ataatgcttt gagtcgtgaa gaagtggcgc atcatgtgag gtgttacatg caatcctgtt
     2161 gcttcattca ctagattgaa gtttctattt agctggtttg gattgatgag accacctttc
     2221 tcgttgatag ttatataatc cttgtgctgt tccttgataa ttcctaactt ttgcttaatc
     2281 ttagaagctt cagctatcag ataatagatc aggtctgttc cgatatcatc aaggcagaca
     2341 tatcgctctg aatccttcgt tttaagccct cctttccctt ttaaggtctg gttgcttcga
     2401 ctgtctctaa gatgcagtat agcccgtccg ctgtcgttct gagtgatgtc cattggacgc
     2461 aatccaaaga cttctcctct tctcaatcca aaaattgtca gataggtcag agcgtagaat
     2521 tgttttggca taatctcttc tgcctttgct atccaagtct tgaactcttt gagagtcact
     2581 ttcttgtttg cagcagggat atcactctgg ccgatgaaaa cacctttcaa gcgatttgag
     2641 agcagatttc catttttgac ggcatcattc agcaatgcca tgaagctgga attgagggtt
     2701 tgaacagtgt atctggtatg gttctgcaac ttttcagcga taaatagttc atactcattt
     2761 ctatccaaat ttttaagcag gatagaacca aactttggct tgatatggtt cttatagaga
     2821 ttgtcattga ggtagtagga agtgtcattc cagcgccctg ttgacaatct cttttcggaa
     2881 tagatatccc aatattgatc aagcgttaga ttcgtattga tacctaattc ttgttcttgg
     2941 atttgttgct caagctctac caaggctgca cgagcttgag ggagagttgt gaaaccactt
     3001 ttactttttt ctcttttttt acctcggaaa aaaaaagaac gtctgacata gtaacgcttg
     3061 cctttagcag tctcatagta atagatattt gggtattttg ttttattata tttcattgta
     3121 ttctccttgt ttatcggctt ctggacaagg tctaaacatt gagaatattg acatcacccc
     3181 tttcatggtg taaaataggg tatagaaaag aggccttttt aatggctgat ttttatacaa
     3241 ggtaagcttc acaatcaaac tttggcgagg gcgattgtgg ggcttttttt attttctgaa
     3301 tagtttgtat agtttataca aactccaagc tgtcaaaggg actgcaagga atggagcgat
     3361 tggaatacca acaactccaa aaaagactaa gaaaagatac agtgctgaaa atatgatttt
     3421 attggcagga agttttttct taggagatgt ttctgaaaga ttaatctcgt ttactatatc
     3481 agcgaaacta tcactaacaa tattttgttc aatatgttca gcttcagttg ttttagtttc
     3541 aatagattca gttttcgtaa cttctttatt gaaaatgtta tcatcaatta tctcagatga
     3601 ttcgctctga attacttctt tttgtgaaga atcaacaatg gtaaggtaaa tttcaaaacc
     3661 aacatccaaa tcattattta tgacaacttt ttcttcataa tcgtcccatt ctttgtatgg
     3721 gccgcctttt atctcacatt ctattaggaa atgatatctt tcatcatcaa aatatttacg
     3781 aatagattta gagactgttg cagggacgta tcctatgtga ttatcaaaaa tcaaaacttt
     3841 tatagcattg ggatcatatt tattatccgt ttctggaatg agttcaatat taaaaatatc
     3901 taaatctcga tatttaaaaa cttttagacc gtattcttca acttcttccc tgatttcctt
     3961 agctgataaa tcgccatagt attttgaata ctctggaatc ccattgtctt cagcaatcat
     4021 attgcaagct tcttttactg cttttttata ttttgtcact ccagcgactc tgaataatat
     4081 tttttcaaca gtcttttgca tatatttccc ctctatataa atatcttcaa tgcaattttt
     4141 aatttactaa tgccaagtac tcttccttga ccatgacttc attagtcaca gttttaagat
     4201 tgtaataaga catgaatttg aggtaatcaa actctgtggg gtcgtctaag ctttctagtg
     4261 cgtcttttac gagatgatgg atcatattcc tatcagcttc gttttcacag cgtaggcgag
     4321 cgttctggta ctctgagcgt gtgtggtctt tgtgtccgag ttcatgcaat agtaccttaa
     4381 ctctctcttt tttgctgagt ttgttagaca agaaagctgt gttggtttct ttttcgtaaa
     4441 atccaagttc atcaggtatt agttcaccgt caaaatcgac aatgcgaacc tgaaaatgac
     4501 ttataatttc tttttcggtc actaagcagt acctctaatc accagcttct ttgagataac
     4561 cttcaatgat agactggatg attttcttct tttcatctgt taattctcgg ccgccaaaca
     4621 tcatgacatt agatgccatt tcttcaacat ttagggtctt cccttgccaa gtgtactctt
     4681 ttgaatcacc agcaatagca ggattatccg tgcgaccaag taaataatct gtggacacgt
     4741 tgaagtagtc agcaatttct tgaagacgtt cagcatttgg ttttttgttt ttcatactat
     4801 agattgtatt tctactatat cctaatgttt cttcaagaga atttatagaa attccacgat
     4861 tttggcaaag ttcttttatt ttttcgaata aagaaaacat tgatttatca acctttctaa
     4921 ggcatgacaa aaaatattta aacttttgat tgtaaatctg ttgacaaaat gcaatctata
     4981 gtttagaata ttatttgtaa gctaaagagt tagcgaacaa gacaactaaa aaataaagcc
     5041 taatgaaact gattggcgtc cgttttctag gtagaacctt acttttagta ggtcttttct
     5101 ctatgattaa attctaaact atagattgtt ttttgtcaag gaattcgcta actttttaga
     5161 taatttttta aaagaaaggg ggagaggaga atggtagagc ctttcttaat tggctttgtt
     5221 ttcggtagtt ttctgtgcta cattgtcgct actttgatag gtgctgtatt ggactttaaa
     5281 cttcagaagt ggcaacaaca aagcgataaa agcaatccaa cctgtaagga ttgaaaactg
     5341 taattctgtg agagtcaagg tagcgacagc tatcactgca gtccctaaac ctacagatga
     5401 aatgtaagtt gtgatttgtg tataaacttt atcaggtatc aattttctat ttcgtccaca
     5461 tgaaatacag taataaattg aaccgaaaaa tataaagcaa agaataagaa cgaatacaat
     5521 agcaaggatt ctttgttctg taaaagcttt caagaaatct gttaacagtg aatcagaaat
     5581 aggatagaag gtagtaaaaa ggtgaaatcc tacgataact gtcaaaatcg gaactaggac
     5641 accatataag atgatttcct ttagatagtg aaaaaatgaa gatttcatta ttctcctc
//
LOCUS       DAVLFB010000104         5433 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLFB010000104 DAVLFB010000000
VERSION     DAVLFB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.104"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            99..758
                     /locus_tag="VXD58_001829"
     CDS             99..758
                     /locus_tag="VXD58_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV0676481.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            783..1850
                     /locus_tag="VXD58_001830"
     CDS             783..1850
                     /locus_tag="VXD58_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV0676482.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELASKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWGVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1953..2963
                     /locus_tag="VXD58_001831"
     CDS             1953..2963
                     /locus_tag="VXD58_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676483.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2975..4666
                     /locus_tag="VXD58_001832"
     CDS             2975..4666
                     /locus_tag="VXD58_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0676484.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4669..5379
                     /locus_tag="VXD58_001833"
     CDS             4669..5379
                     /locus_tag="VXD58_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV0676485.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1784 a          770 c         1047 g         1832 t
ORIGIN      
        1 aaaaaaaatg tttgacagcg cttgacaaag gaaacggttt cattgtataa tgtaaacata
       61 gtgacaccga tgtcaaatat aaaaaagaag gtctttatat gaaaaactta acaaaaatta
      121 agttcaaaga aaatggggaa tttaatcatt tccctgggaa tacagttgta gcaaatcttt
      181 atactaaaca agatttgatg gaagttgttg atattattca atcacgttat agagaattac
      241 catttatcga taagtttact ttaactccaa ggaattctat tcacatgact gtaattgaat
      301 tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag taatcttcct ctagatacac
      361 ctttacagga aatacatgat tactttgcta aacaacttga aatttttcca ttgttggatg
      421 aagaaattca tatgcgtata actgaaatgg gaaaacaaaa catactagtt gaacctgcag
      481 atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta tgtctcagaa aaagcaggtg
      541 tttgtttccc taatcatgat agatatcaat tccatatttc aattgggtat cttcggattc
      601 ctctaaccga agaggaggaa gaagagttta ctaaagtcag agcagaatta actgaaattt
      661 tattagagaa gattccaact attactgtaa accgtattga ctatactgta tttgaagata
      721 tgagacaatt tgttccatat cacgaaaaat ttaaataaaa taataaaaaa ggagaactta
      781 tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac gggagttgct cttactacac
      841 ttgctgcttg ctctggaggt tcaaattcta cgactgcttc ttcatctgaa gaaaaagctg
      901 ataaaagtca agaattagtt atctattcga actcagtctc aaatggtcgt ggtgattggt
      961 taactgctaa agcaaaagaa gctggtttta atataaaaat ggttgatatc gctggcgctc
     1021 aattagcaga ccgtgttatt gctgagaaga ataatgcagt tgcagatatg gtatttggaa
     1081 ttggtgctgt tgactcaaat aaaattagag atcaaaaatt actagtacag tacaagccta
     1141 aatggttaga taaaattgat caatctttat cagataaaga taattattat aatcctgtga
     1201 ttgttcaacc attagtttta attggggcgc ctgatgtaaa agaaatgcct aaagactgga
     1261 cagagttagc tagtaagtat aaaggtaaat attcaattta tggtcttcaa ggtggtacag
     1321 gacgggcaat tctagcaagt atcttagttc gttaccttga tgaaaaagga gatttaggcg
     1381 tatcagaaaa aggatgggga gttgcaaaag aatactttgc aaatgcctat actcttcaaa
     1441 aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag cccaattcaa tacggtatga
     1501 tctggggttc tggagcgtta atcggacaaa aagaacaaaa tgttgaattc aaagttatga
     1561 ctcctgagat tggtgtacca tttgtaactg aacaaactat ggttttaagc actagtaaaa
     1621 aacaagcgtt agctaaagaa tttattgatt ggtttggtca atcagaaatt caagtagaat
     1681 atagtaaaaa atttggatct attcctgcaa ataaagatgc cctaaaagag ttacctgaag
     1741 atacgaaaaa atttgttgat caagtgaaac cacaaaatat tgactgggaa gctgttggaa
     1801 agcatttgga tgaatgggta gaaaaagctg aattagaata cgtaaaataa gaaataagag
     1861 ttatttttta tgtccaagtc tctctattat ttctaaattt aaggaagaaa taaagagagg
     1921 cttttggttt agtaatagag ggagttagtg agatgattaa atttgataat attcaaatta
     1981 aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa ggggaattct
     2041 ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca ttggtaggtt
     2101 ttttagatcc atcatcagga agtattgaag ttaatggaac agatgtcact catttggaac
     2161 ctgaaaagcg tggaattggt attgtatttc aatcttatgc gctatttcca actatgactg
     2221 tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat gttattaaag
     2281 ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta cagcgtaatg
     2341 tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct ctggttcttg
     2401 aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa ttacgtgtag
     2461 atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact ttatatgtta
     2521 ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt aacaatggat
     2581 acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact gaatttgtat
     2641 gtgattttat tggagatatt aatgttttga ccgatgaaac agtccacgaa gtattactga
     2701 aaaatacaag cgttttctta gaggataaaa aaggttacat tcgattagag aaagttcgat
     2761 tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat gttgagtttt
     2821 ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt aatgtaacaa
     2881 gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta tttatcacac
     2941 catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa atttaaaaga
     3001 ttggcttatt cgtttagggt taatctggtt cttagtaaca tttattattt atccaaactt
     3061 tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg ctgtacatcg
     3121 tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt tagcattttc
     3181 actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag agtactttga
     3241 tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt atggaggagt
     3301 ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta caaaattttt
     3361 acaaaatgtt atcccttctt tagactctaa ctggtttatt gggtatggtg cagtcttatt
     3421 cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata caattcgaag
     3481 cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat ttactgtttt
     3541 ccgaaaagta gtgttaccaa ccttaattcc aactctattt gcacttacta ttatggtttt
     3601 tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag aatttcaaac
     3661 tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt tagctgcctt
     3721 acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga ataagataga
     3781 aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa aacaaaaaat
     3841 tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt tcacagtttt
     3901 catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg caattcaaac
     3961 aggtaactta acattatcaa actttacttt agaaaattat cgcttattct ttagtaatag
     4021 tgcggcattc tctccattct tggtcagctt tatttattct attattgctg cgacaacagc
     4081 aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc gttttgattt
     4141 cttatttgaa tatggtgctc tacttccttg gttactacca agtacacttt tagcagtaag
     4201 tttattattt acttttaatc agccacaatt tcttgtcttg aatcagattt tggtaggtag
     4261 tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt atagaatggt
     4321 acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa gtatgggtgc
     4381 ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg ttgttctctc
     4441 tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg tattccttta
     4501 ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg aaacagcaac
     4561 atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatca tttctggaac
     4621 ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat gacagccact
     4681 agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt agtcattggt
     4741 agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg aacttatagt
     4801 attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc ttataaattt
     4861 gggacaggag atcctacacg attaggagct caagttatat caggtgtggg ttttctaggc
     4921 gctggaacga ttcttattac agataaaaag aaaattacag gtctgacaac tgcagcaggc
     4981 atttgggctt cggcaggaat tggattagct attggagtag gtttttatga gggagctctt
     5041 ttagtagcca tttctgtttg gggtgtgata tccatgttcc aaccactaaa aaaatatctg
     5101 caaaatcgtt ctaaaatgat tgaattgtat atagtggtta aatctacaga agcctataat
     5161 cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag aacagcgttt
     5221 ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa aatagcgtca
     5281 tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga agaaattgct
     5341 aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa caaagggaca
     5401 gatgtttttg tccttttgtt ttttagatgt ttt
//
LOCUS       DAVLFB010000105         5428 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLFB010000105 DAVLFB010000000
VERSION     DAVLFB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.105"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            62..745
                     /gene="rpiA"
                     /locus_tag="VXD58_001834"
     CDS             62..745
                     /gene="rpiA"
                     /locus_tag="VXD58_001834"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV0676486.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            759..1970
                     /locus_tag="VXD58_001835"
     CDS             759..1970
                     /locus_tag="VXD58_001835"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV0676487.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            1972..2505
                     /locus_tag="VXD58_001836"
                     /pseudo
     CDS             1972..2505
                     /locus_tag="VXD58_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            2529..3338
                     /locus_tag="VXD58_001837"
     CDS             2529..3338
                     /locus_tag="VXD58_001837"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV0676488.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3814..4209
                     /locus_tag="VXD58_001838"
     CDS             3814..4209
                     /locus_tag="VXD58_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV0676489.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4219..4794
                     /locus_tag="VXD58_001839"
     CDS             4219..4794
                     /locus_tag="VXD58_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV0676490.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            4819..5331
                     /locus_tag="VXD58_001840"
     CDS             4819..5331
                     /locus_tag="VXD58_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV0676491.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         1661 a          967 c         1264 g         1536 t
ORIGIN      
        1 tagatgaaat aaaagccttt acatggtata atagaggtag ctctttaatg gaggtgtttg
       61 agtggaaaat ctgaagaaaa tggcaggtat caaggctgct gagtttgtca aggatggaat
      121 ggtcgttgga cttggaacag gctcgactgc ctattatttt gtcgaagaaa tcggtcgtcg
      181 tatcaaggaa gaaggattgc agattatagc tgtaacgact tctagtgtga ccactaaaca
      241 ggcagaaggc cttaatatcc cactcaaatc tattgaccaa gtagactttg tcgatgtgac
      301 agttgatggc gcagatgaag tggatagtca gtttaatgga atcaaaggtg gtggtggtgc
      361 tcttctgatg gagaaggttg ttgcgacacc atcaaaagaa tacatttggg tggtggatga
      421 aagcaagctg gtcgaaaaac taggtgcttt taaattgcca gtagaagtgg ttcagtatgg
      481 tgcagaacag gtctttcgtc gttttgaacg agctggctac aaaccaagtt tccgtgaaaa
      541 agacggccaa cgttttgtga cggatatgca gaattttatc attgacctcg ccttggatgt
      601 cattgaaaat ccaattgctt ttggacaaga attggaccat gtcgttggtg ttgtggagca
      661 tggtttattc aaccaaatgg tggataaggt catcgttgct ggacgggatg gagttcagat
      721 ttcaacttca aaaaaaggaa aatagaaggg ggcataagat gtctaaattt aatcgtattc
      781 atttggtggt actggattct gtaggaatcg gtgcagcacc agatgctaat aactttgtca
      841 atgcaggggt tccagatgga gcttctgaca cactgggaca catttcaaaa acagttggtt
      901 tgaatgtccc aaacatggct aaaataggtc ttggaaatat tcctcgtgaa actcctctta
      961 agactgtagc agctgaaagc aatccaactg gatatgcaac aaaattagag gaagtatctc
     1021 ttggtaagga tactatgact ggacactggg aaatcatggg actcaacatt actgagcctt
     1081 tcgatacttt ctggaacgga ttcccagaag aaatcctgac aaaaatcgaa gaattctcag
     1141 gacgcaaggt tattcgtgaa gccaacaaac cttattcagg aacggctgtt atcgatgatt
     1201 ttggaccacg tcagatggaa actggagagt tgattatcta tacttcagct gaccctgttt
     1261 tgcagattgc tgcccacgaa gacattattc ctttggatga attgtaccgt atctgtgaat
     1321 acgctcgttc gattaccctt gagcgtcctg cccttcttgg tcgcatcatt gctcgccctt
     1381 atgtaggtga accaggtaac ttcactcgta cggcaaaccg tcgtgacttg gctgtatctc
     1441 cattttcccc aactgttttg gataaattga atgaggctgg tatcgatact tatgctgtgg
     1501 gtaaaatcaa cgatatcttt aacggtgctg gtatcaacca tgacatgggt cacaacaagt
     1561 caaatagtca tggaattgat acactattga agactatggg acttgctgag tttgaaaaag
     1621 gattctcatt cacaaaccta gttgactttg atgcccttta cggccatcgt cgtaatgctc
     1681 acggttaccg tgattgcttg catgagtttg atgaacgctt acctgaaatt atcgcagcta
     1741 tgagagagaa tgaccttctc ttgattactg cggaccatgg aaatgaccca acgtatgcag
     1801 gaacggatca cactcgggaa tatattccat tgttggccta tagccctgcc tttaaaggaa
     1861 atggtctcat tccagtagga cattttgcag atatttcagc gactgttgcc gataactttg
     1921 gtgtggaaac tgctatgatt ggggaaagtt tcttagataa attggtataa gatgacgcgc
     1981 tatgctttgc tggtgagagg tatcaatgtt ggtggtaaga ataaggtcgt catggcggag
     2041 cttcgtcaag aattgacaaa cttgggactg gaaaaggttg agagctacat caacagtggc
     2101 aatattttct ttacttcgat agattccaaa gcccaattgg ttgaaaagct agagactttc
     2161 tttgcagtcc attatccatt tattcagagc ttttctttac tgagtctaga ggactttgag
     2221 gcggaacttg aaaatctacc agcttggtgg agcagagatt tggcacgaaa agattttctc
     2281 ttttacactg agggtttgga tgtggaccaa gtcatcgcga cagttgaaag tttagagctg
     2341 aaagatgaag tgctttattt tggaaaactt gggattttct aggggaaatt ttctgaagaa
     2401 tcctattcta agactgccta tcataagtac ttgctgaggt gcctttctac cgccacatta
     2461 ctattcgtaa tgctaaaacc tttgacaaaa ttggtcaaat gctaaaaaaa taataaagga
     2521 gacacacaat gacattttta aacaaaatcc atgaaactgc tactttcctg aaagaaaagg
     2581 gaattgcagc ccctgagttc ggtctaatcc ttgggtcagg acttggagaa ttggcagaag
     2641 aaatcgaaaa tccagttgta gtagactatg ctgagattcc aaactggggc cgttcaacag
     2701 tagtcggtca tgctggtaaa ttggtatatg gtgaactggc aggtcgcaag gtcttggctc
     2761 ttcaagggcg tttccatttc tatgaaggga atcctctgga agtggtgact ttcccagttc
     2821 gtgtgatgaa agttcttgga tgtgaaggtg ttattgtaac caatgcagct ggcggtatcg
     2881 gatttggtcc tggtaccttg atggctatct cagaccatat caacatgacg gggcaaaatc
     2941 cattgatggg tgaaaacttg gatgactttg gcccacgttt cccagatatg tctagggcct
     3001 acacaccaga ataccgtgct actgcccatg aagtggctaa aaaacttaat atcaagcttg
     3061 atgaaggtgt ctatatcgga gttactggtc cgacttatga aacaccagca gaaattcgtt
     3121 cctataagac actgggagca gatgcagttg gtatgtctac ggttcctgaa gttatcgtgg
     3181 cagcccactc tggcttgaaa gttctgggaa tttcatgtat cactaacttt gcggctggtt
     3241 tccaagaaga actcaatcac gaagaagttg tagaagtgac tgaacgtgtt aaaggtgatt
     3301 ttaaaggctt gcttaaagcg attcttgctg aattgtaaga aaaaagattt aaaaggggga
     3361 gtgcctctgt cttttcagga ttgactgcct atccgtatta aagaagaaac agaggaatac
     3421 tatgagcttc ttcctgctct tataactgaa agaagcggaa gaataggtgt gtctgatctg
     3481 atagccagca ttgtgaaaga caagattcta ggatactagc attagcttcc tagccaagca
     3541 gactagtatg ataaggagag atgagaatga attgactttc tgaatttctc agtcttatca
     3601 tatatagcac aatgagattt cgcttgagtc tgcttgtaaa taaacgaaaa gaaagataag
     3661 aaataatgaa aattggtcaa cgaattatgc gctttggcat aaaaaattaa gtatcggagt
     3721 tgtatctgtt gtagtcggct ttgatttcta gctccagctg gaatttcagc caatgaagta
     3781 aagcaagatg taacaactga agtggtaata ggtgtgctag attctaagga ggaattgaaa
     3841 gagtcagaaa atgatgctcc aaaactagaa actcctctta gagaggagcc aagactagct
     3901 cctcaaacgc ttccggaagc aagtgaagtt cttgaaaaca aaagggaaga gtcaaaagta
     3961 gagataacag agccagctca agcggatgac atccgcaagg ttgttgggga attagccaag
     4021 gatataagta ttactaagtt gtatatgaca ggtcattctc ttggaggcta cctagctcag
     4081 attgcagcgg ttgaagatta ccaaaaatat cctgattttt ataaccatgt attgaggaaa
     4141 gtgacaactt tcagtgctcc taaagtgatt acttccagaa ctgtttggga tgctaagaat
     4201 ggtttctgag atgttggttt ggaaagtcgt aaattagctg ttagtggaaa aattaagcat
     4261 tatgtggttg ataatgacaa tgttgtgact cccttgattc ataataatcg tgatattgtt
     4321 acatttacag gtaattcacg ctttaaacac cgttctcgtg gctattttga aagtccaatg
     4381 aatgatattc ctaactttaa tattggtaaa caagctacct tggataaaca tggttatcgt
     4441 gatccgaaat tggataaagt gcgattcttt aagaaacagg ctctacctca atcttctagt
     4501 caaccaagcg ctgaaccaat ggaaaatatt gccttaggaa aacaggttac tcaaagttcg
     4561 acagctttcg gaggagatgc tagaagagct gtggatggca aagtcgatgg taactatggt
     4621 cacaattctg tcactcatac aaacttccaa tctaagcctt ggtggcaagt agatttggct
     4681 aaagaagaaa ccattcgcca aatcaatatt tacaaccgaa cagacactgc ccaggataga
     4741 ttggcaaact ttgatgtcat tcttttagac agttctggta aagaaattga gtgaaaacgt
     4801 ataatatctc ctaaagatgt gtcagcacaa attacgatta accataaaaa agcgcgctat
     4861 gttcggattg ggctagaagg ctataatgcc ctcagtcttg cagaagttga agttttccgc
     4921 tttatagcta cgaatgctga aacggcgaca caagtttcta agccagttca accaatcagt
     4981 cagactcctg tgaaagataa aacattgaca attcaacaca gtggagctta cattgcccgc
     5041 tactccataa cttgggaaga agttccagta gataaagatg gaaaccaagt tgttcgtagt
     5101 cattcttggg aaggaaacgg ttgcaaccag actgcaggtt ttgtcctcaa cttcccaatc
     5161 aaagaaaata tgagaaatct gcgagttaag attgagaaaa agacgggcct actatggaat
     5221 agatggcaaa caatctatga aaacagacca attttagctc aaccccaccg taaaattacc
     5281 cattggggta cgacattgaa ttccaaggtg agtgacgatg atgtcttgta atctgatggt
     5341 acaatgacag ttagtttgtc tagtttataa gaaagtacta tctgagcttg aatagaactc
     5401 aggtagctct ctgtgaaaga acaaaatt
//
LOCUS       DAVLFB010000106         5076 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLFB010000106 DAVLFB010000000
VERSION     DAVLFB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.106"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            206..1751
                     /locus_tag="VXD58_001841"
     rRNA            206..1751
                     /locus_tag="VXD58_001841"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1803..1875
                     /locus_tag="VXD58_001842"
     tRNA            1803..1875
                     /locus_tag="VXD58_001842"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1836..1838,aa:Ala,seq:tgc)
     gene            2000..4901
                     /locus_tag="VXD58_001843"
     rRNA            2000..4901
                     /locus_tag="VXD58_001843"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4978..>5076
                     /gene="rrf"
                     /locus_tag="VXD58_001844"
     rRNA            4978..>5076
                     /gene="rrf"
                     /locus_tag="VXD58_001844"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1436 a         1028 c         1488 g         1124 t
ORIGIN      
        1 cttaaaaatc ttcaaaaaag tgttgacaag tgaaagtggc tgtgatatac taatatagtt
       61 gtcgcttgag agaagcaagt gacaaagacc tttgaaaact gaacaagacg aaccaatgtg
      121 cagggcgcta caacataagt tgtagtactg aacaatgaaa aaacaataaa tctgtcagtg
      181 acagaaatga gtaagaactc aaacttttaa tgagagtttg atcctggctc aggacgaacg
      241 ctggcggcgt gcctaataca tgcaagtaga acgctgaagg aggagcttgc ttctctggat
      301 gagttgcgaa cgggtgagta acgcgtaggt aacctgcctg gtagcggggg ataactattg
      361 gaaacgatag ctaataccgc ataagagtag atgttgcatg acatttgctt aaaaggtgca
      421 cttgcatcac taccagatgg acctgcgttg tattagctag ttggtggggt aagggctcac
      481 caaggcgacg atacatagcc gacctgagag ggtgatcggc cacactggga ctgagacacg
      541 gcccagactc ctacgggagg cagcagtagg gaatcttcgg caatggacgg aagtctgacc
      601 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa agctctgttg taagagaaga
      661 acgagtgtga gagtggaaag ttcacactgt gacggtatct taccagaaag ggacggctaa
      721 ctacgtgcca gcagccgcgg taatacgtag gtcccgagcg ttgtccggat ttattgggcg
      781 taaagcgagc gcaggcggtt agataagtct gaagttaaag gctgtggctt aaccatagta
      841 ggctttggaa actgtttaac ttgagtgcaa gaggggagag tggaattcca tgtgtagcgg
      901 tgaaatgcgt agatatatgg aggaacaccg gtggcgaaag cggctctctg gcttgtaact
      961 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgct
     1021 gtaaacgatg agtgctaggt gttagaccct ttccggggtt tagtgccgta gctaacgcat
     1081 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc
     1141 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc
     1201 ttgacatccc tctgaccgct ctagagatag agttttcctt cgggacagag gtgacaggtg
     1261 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca
     1321 acccctattg ttagttgcca tcattcagtt gggcactcta gcgagactgc cggtaataaa
     1381 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg
     1441 tgctacaatg gctggtacaa cgagtcgcaa gccggtgacg gcaagctaat ctcttaaagc
     1501 cagtctcagt tcggattgta ggctgcaact cgcctacatg aagtcggaat cgctagtaat
     1561 cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac
     1621 cacgagagtt tgtaacaccc gaagtcggtg aggtaaccgt aaggagccag ccgcctaagg
     1681 tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga
     1741 tcacctcctt tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt
     1801 gtggggcctt agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga
     1861 tcccgctagg ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct
     1921 atatcaaata gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga
     1981 ctgaaaggtc agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta
     2041 ggagccgacg aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg
     2101 atccagggat ttccgaatgg gggaacccaa caggtaatac ctgttaccta catctgttaa
     2161 ggatgtgagg aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc
     2221 aaaagcgatt gccttagtag cggcgagcga aacggcagaa gggcaaaccg aagagtttac
     2281 tcttcggggt tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag
     2341 atcagccaaa gagagtaata gcctcgtatt taaaatagtc tttgtactta gcagtatcct
     2401 gagtacggcg ggacacgtga aatcccgtcg gaatctggga ggaccatctc ccaaccctaa
     2461 atactcccta gtgaccgata gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg
     2521 gaggggagtg aaatagaacc tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg
     2581 gtgagagcgt gccttttgta gaatgaaccg gcgagttacg ttatgatgcg aggttaagtt
     2641 gaagagacgg agccgtaggg aaaccgagtc tgaatagggc gccttagtat catgacgtag
     2701 acccgaaacc atgtgaccta cccatgagca ggttgaaggt gcggtaagac gcactggagg
     2761 accgaaccag ggcacgttga aaagtgcttg gatgacttgt gggtagcgga gaaattccaa
     2821 acgaacttgg agatagctgg ttctctccga aatagcttta gggctagcgt cgacattaga
     2881 gattcttgga ggtagagcac tgtttgggtg aggggtccat cccggattac caatctcaga
     2941 taaactccga atgccaatga attatggtcg gcagtcagac tgcgagtgct aagatccgta
     3001 gtcgaaaggg aaacagccca gaccaccagc taaggtccca aaataattgt taagtggaaa
     3061 aggatgtggg gttgcacaga caactaggat gttagcttag aagcagctat tcattcaaag
     3121 agtgcgtaat agctcactag tcgagtgacc ctgcgccgaa aatgtaccgg ggctaaaaca
     3181 atttaccgaa gctgtggata cctttatagg tatggtagga gagcgttcta tgtgtgatga
     3241 aggtataccg tgaggagtgc tggaacgcat agaagtgaga atgccggtat gagtagcgaa
     3301 agacaggtga gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc
     3361 ctgggttagt cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat
     3421 attcctgtac tagagtatgt agtgatggag ggacgcagta ggctaactaa agcagacgat
     3481 tggaagagtc tgtctaagca gtgaggtgtg aattgagtca aatgcttaat tctataacat
     3541 tgagctgtga tggggagcga agtttagtag cgaagttagt gacgtcacac tgccaagaaa
     3601 agcttctagc gtttaaacat actctacccg taccgcaaac cgacacaggt agtcgaggcg
     3661 agtagcctca ggtgagcgag agaactctcg ttaaggaact cggcaaaatg accccgtaac
     3721 ttcgggagaa ggggtgctga cttaaagtca gccgcagtga ataggcccaa gcaactgttt
     3781 atcaaaaaca cagctctctg ctaaatcgta agatgatgta tagggggtga cgcctgcccg
     3841 gtgctggaag gttaagagga gtgcttagcg taagcgaagg tatgaattga agccccagta
     3901 aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg
     3961 acccgcacga aaggcgtaat gatttgggca ctgtctcaac gagagactcg gtgaaatttt
     4021 agtacctgtg aagatgcagg ttacccgcga caggacggaa agaccccatg gagctttact
     4081 gcagtttgat attgagtgtc tgtaccacat gtacaggata ggtaggagtc taagagatcg
     4141 ggacgccagt ttcgaaggag acgctgttgg gatactaccc ttgtgttatg gccactctaa
     4201 cccagatagg tgatccctat cggagacagt gtctgacggg cagtttgact ggggcggtcg
     4261 cctcctaaaa ggtaacggag gcgcccaaag gttccctcag aatggttgga aatcattcgc
     4321 agagtgtaaa ggtataaggg agcttgactg cgagagctac aactcgagca gggacgaaag
     4381 tcgggcttag tgatccggtg gttccgtatg gaagggccat cgctcaacgg ataaaagcta
     4441 ccctggggat aacaggctta tctcccccaa gagttcacat cgacggggag gtttggcacc
     4501 tcgatgtcgg ctcgtcgcat cctggggctg tagtcggtcc caagggttgg gctgttcgcc
     4561 cattaaagcg gcacgcgagc tgggttcaga acgtcgtgag acagttcggt ccctatccgt
     4621 cgcgggcgta ggaaatttga gaggatctgc tcctagtacg agaggaccag agtggactta
     4681 ccgctggtgt accagttgtc ttgccaaagg catcgctggg tagctatgta gggaagggat
     4741 aaacgctgaa agcatctaag tgtgaaaccc acctcaagat gagatttccc atgattatat
     4801 atcagtaaga gccctgagag atgatcaggt agataggtta gaagtggaag tgtggcgaca
     4861 catgtagcgg actaatacta atagctcgag gacttatcca aagtaactga gaatatgaaa
     4921 gcgaacggtt ttcttaagtt gaatagatat tcaattttga gtaggtatta ctcagagtta
     4981 agtgacgata gcctaggaga tacacctgta cccatgccga acacagaagt taagccctag
     5041 aacgccggaa gtagttgggg gttgccccct gtgaga
//
LOCUS       DAVLFB010000107         5073 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLFB010000107 DAVLFB010000000
VERSION     DAVLFB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.107"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            90..362
                     /gene="rpsP"
                     /locus_tag="VXD58_001845"
     CDS             90..362
                     /gene="rpsP"
                     /locus_tag="VXD58_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV0676492.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            382..621
                     /locus_tag="VXD58_001846"
     CDS             382..621
                     /locus_tag="VXD58_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV0676493.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            1198..1986
                     /locus_tag="VXD58_001847"
     CDS             1198..1986
                     /locus_tag="VXD58_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV0676494.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            2017..2535
                     /gene="rimM"
                     /locus_tag="VXD58_001848"
     CDS             2017..2535
                     /gene="rimM"
                     /locus_tag="VXD58_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV0676495.1"
                     /translation="MNYFNIGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            2525..3244
                     /gene="trmD"
                     /locus_tag="VXD58_001849"
     CDS             2525..3244
                     /gene="trmD"
                     /locus_tag="VXD58_001849"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV0676496.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            3256..3594
                     /locus_tag="VXD58_001850"
     CDS             3256..3594
                     /locus_tag="VXD58_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676497.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(3625..3945)
                     /locus_tag="VXD58_001851"
     CDS             complement(3625..3945)
                     /locus_tag="VXD58_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676498.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            4043..4258
                     /locus_tag="VXD58_001852"
     CDS             4043..4258
                     /locus_tag="VXD58_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV0676499.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(4327..4413)
                     /locus_tag="VXD58_001853"
                     /pseudo
     CDS             complement(4327..4413)
                     /locus_tag="VXD58_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4464..5000)
                     /locus_tag="VXD58_001854"
     CDS             complement(4464..5000)
                     /locus_tag="VXD58_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV0676500.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         1692 a          876 c         1080 g         1425 t
ORIGIN      
        1 acatgtcaga aatctgctat aatagaacat gtgctaaata gctcagctat ttcaccgaaa
       61 taaaaaatta aagaaatgag actttaaaaa tggcagttaa aatccgtttg actcgtatgg
      121 gttctaagaa aaaacctttc taccgtatca acgtagcaga ttcacgttca ccacgtgacg
      181 gacgtttcat cgaaacagtt ggaacttaca acccacttgt tgctgaaaac caagtaactt
      241 tgaaagaaga ccgcgttctt gcatggttgg ctaatggagc tcaaccttca gacacagtac
      301 gtaacatcct ttcaaaagaa ggcgtattga aaaaattcca cgattctaaa ttttcaaaat
      361 aagtttaaag taggttgaca gatggatacg attgaaaatc tcattattgc gattgtgaaa
      421 cccttaattt cacaaccaga tgccttaact atcaagattg aggatacacc agaatttttg
      481 gaatatcatt tgaatcttga tcaaagcgat gtgggtcgtg taatcggtcg taagggtcgc
      541 actatttctg cgataagaac gattgtctac tctgtcccaa ctgaatacaa aaaagtaaga
      601 atcgttattg acgaaaaata agaagggcgg gacggatgtc tcgctttttt gtgtggaata
      661 gaagatatag taaaatgaaa caagaacagg acaaatcgat caggacagtc aaatcgattt
      721 ctaacaatgt tttagaagta aaggtgtact attctagttt caatatacta taaaagattg
      781 aacgtttagg caattccaag actacttaat cgaacattaa cagcagtctc ggactgatga
      841 aggtctcttt atgaagttag tggaggaagt cggaaaagta gccgaggttt tgaataggcg
      901 ctctggtcga aaagaaggga taggattcga acgaggaact tgccagagaa ctggctgata
      961 tcatttacta caccgtcgca atcgtagcta ttaatcatat tgaccttacc aagactgtct
     1021 ttgagaaaga caaaaccgca gccgttaatt accaacataa acataatttg gaaggattat
     1081 tgaagggaaa gtagagatgt tgtccccttc ctccaacttg agaaaatcct tggaacgtga
     1141 taaaataaag gataatgatt tttaataatt cattatatag aatgagtgga tttctttatg
     1201 aagaggtttg aagtatctac agaaattggt agcctctctg ttacttatca aaagcaaaag
     1261 aaagtgctag tttgtctaaa cggagcaggt ttgctaccaa gttatgaaaa tttttcactt
     1321 atacttgaaa aacctcctcc tacaattggt tatttgacaa ttgattttcc gaacacaggt
     1381 aggagtccga ttcatgacca agctggaaaa aatctagata atcttgcaga tgcggtttat
     1441 gaagtacttg aagaattagg aatttctgaa tatatacttt gtgcacatag ttggagtgga
     1501 attttagctt gcaaattact cgagaaacca attaagcgtc agactttagt agcaattgaa
     1561 ccgacaacta aaaaagtcat gtttgctgat ttttcagaaa atccttatcc aaaaatggaa
     1621 gagcagatga ggctgattga cgagtgtggt cctgaacttt attttaagaa cttaactcaa
     1681 gcaacattta gtcctgaaac gaataaaaaa atctgggaat taatgcaaga aaaaggctta
     1741 gagttggaaa atcaagatcc agaatttcag atatctggag agattactga ggaagatttt
     1801 gagaatttgt cgatagaatc tcatgtccct gtttttattt tttgtcagac ttatagagaa
     1861 aaagagtaca gagaatcaga atattggact tccaatacta aactcatttt aggagggaat
     1921 caccattatt tacaatggtc agaatcggaa aaaattgcgg ctattattcg agaattgtca
     1981 gaataagatg gaaaaaagga gattacagga gacaagatga actactttaa tattgggaaa
     2041 atcgttaata cgcagggatt acagggtgag atgcgagtct tgtctgtgac ggattttgca
     2101 gaagaacggt ttaaaaaagg agctgagctg gctttgtttg atgaaaaaga tcagtttgtc
     2161 caaacagtga ccatcgctag ccaccgtaaa cagaagaact ttgacattat taaattcaaa
     2221 gatatgtacc atatcaatac tatcgaaaag tacaagggat acagtctcaa ggtcgctgag
     2281 gaagatttga atgacctaga cgatggtgaa ttttactatc acgagattat cggtttggaa
     2341 gtctatgagg gtgatagctt ggttggaacc atcaaggaaa tcctgcaacc aggtgctaat
     2401 gatgtctggg tggtcaaacg aaaaggcaaa cgtgatttgc ttttacctta tatcccacca
     2461 gtggttctca atgttgatat tccaaataaa cgggtcgatg tggaaatctt agaagggtta
     2521 gacgatgaag attgatattt taaccctctt tccagagatg ttttctccac tggagcactc
     2581 aatcgttgga aaggctcgag aaaaagggct cttggatatc cagtatcata attttcgaga
     2641 aaatgctgaa aaggcccgtc atgtagatga tgatccctac ggaggcggtc agggcatgtt
     2701 gctcagagca caacctattt tcgattcctt tgatgctatt gaaaagaaaa atccgcgcgt
     2761 tattctcctt gatcctgctg gaaagcagtt tgatcaggct tatgctgaag atttggctca
     2821 agaggaagag ctaatcttta tctgtgggca ctatgagggt tatgatgagc gcattaagac
     2881 cttggtaaca gatgagattt ccctaggcga ctatgtcctc actggtggag aattggcagc
     2941 tatgaccatg attgatgcta cagttcgcct gattccagaa gtgattggca aggagtctag
     3001 ccaccaagat gatagttttt cttcaggtct tttagaatat cctcagtaca cacgtcccta
     3061 tgattatcga ggcatggtcg tgccagatgt attgatgagt ggccaccatg aaaagattcg
     3121 tcagtggcga ttgtacgaga gtttaaagaa aacctacgag cgcagaccgg atttacttga
     3181 acattatcaa ctgacagtag aagaagaaaa aatgctggca gaaatcaaag aaaacaaaga
     3241 ataaaggaga aacctatgca agtaatcaaa cgtaatggcg aaattgctga atttaatcca
     3301 gataagattt accaagccat cttgaaggca gcccaaactg tttatgtttt gacagatgat
     3361 ttgcgtcaaa atcttgctca agtcactaag aaggtagttt tggatttaca agaagccaag
     3421 gtggaacgtg cgactatcag tatgattcaa tctatggttg aacatcgttt attgggcgca
     3481 ggttacatta ccattgcaga acactacatt tcctatcgtt tacaacgtga cttggaaaga
     3541 agtggttatg gagatcatat cgcagttcat ttacattttg aacaaattcg ctaagaaaaa
     3601 agagtgggat gaagcaacta catctcactc tttcttaaat ctattttttt gggctgtttg
     3661 gacagttgag gggacaaatc gcaggcgttg aatatcgctt atacggataa tccggctcac
     3721 atttttggca aaatttttca cgataatttg ctggccttgc tgatcatatt tgatgatatc
     3781 acctgtaaag cttgtctcag acaagataag gtgaacagcg gtttgtttct ggatagcctc
     3841 ttcaatagtg gctgtaaggg agttggtttc agtctggtca ggttggtcat gtccatttaa
     3901 aaaatcatgt attttatcta gaacttgctg gaatttatgt ctcatattgg cctcccttct
     3961 tctatatcaa tattatataa aattttccta aaatctacaa gattttacga ataaactaat
     4021 gaaactaaaa aaggagaaga aaatggcaga atttacattt gaaatcgaag agcacttgtt
     4081 gactctttct gaaaacgaaa aaggttggac caaggaaatt aaccgtgtga gctttaatgg
     4141 tgcccctgca aagtttgata ttcgtgcttg gagtccagac catactaaaa tgggcaaagg
     4201 gattactctc tcaaatgaag aatttcaaac gatggtggat gcctttaaag gcaactaata
     4261 ctcttcgaaa atctcttcaa accgcatcag ctttgccttg ccgtatgtat ggttactgac
     4321 ttcgtcagtt tcatccacaa cctcaaaaca gtgttttgag cagcctgcgg ctagcttcct
     4381 agtttgctct ttgattttca ttgagtataa gttttcaaaa taaaaaaaaa ggatattgag
     4441 tcatgataac tcgatatcct tttttagtta gtaaagtaag cctgattttt aaggcgttga
     4501 agtaggggac ggctaataaa actaatagcc agaagtttgc caaggtctgg aatgataaag
     4561 ggaagaaccc ccacaacaag agctttttca aatgccattc cagctaggaa atgcaagctg
     4621 agaatcccgc cgacaaagac aagggcatca cccaagaggt ttgcaagaaa aatcttaaca
     4681 acaccactct tgctgttggt tagagaggaa gtaagtccag agtaaacgag ataaaaccaa
     4741 agatagcctg cagtagggcc aactaaagcc tgaaaaccag ctccacctcc tgcaaagaca
     4801 ggaagaccga tagcacctag aagaagatag agtccagcag aaagtacagc ctctctcggt
     4861 ctaaagacag tagcaatcaa gccgattgca aagttttgca gagtgaaggg aacaggtcca
     4921 attggaagac tgatttgtgc caaaacagca atgagagcag ccccaatagc agggatagca
     4981 taaacgtgag cttttttcaa aatagttaac ctcttttcta atgtatagta actattatac
     5041 tcatatagga gtcgatgtca accatttttt aaa
//
LOCUS       DAVLFB010000108         4995 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLFB010000108 DAVLFB010000000
VERSION     DAVLFB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.108"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..1135)
                     /locus_tag="VXD58_001855"
     CDS             complement(<1..1135)
                     /locus_tag="VXD58_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV0676501.1"
                     /translation="MIKRKIYIICVLLSYFLPSFFTVGSMATLFALLSTLVKLFVTFD
                     YIPKVLLQHKVTLIDILVVLFLFFQAFAAFQSQTLYLNYVGGQFFLLGLYSFLKHFLL
                     LDCKTTIKSLFLTFILFLCVQVITQLLFPVGFDSLHPTGDNRLYFLGRKNIATPYIIV
                     GLGGFYLLNKKMNEFISLKEIIFLGLFGILSFLTQSSTAIICYGLFIFIRLLGLKENI
                     GKLYSLVSMAVYVCFSLSIIFSQSTILSTFTAIFSKNATFSGRINIWQLAIRIFEENF
                     WFGRGLNVNFNAWTNGIIVNSAHNTLLDILARTGIFPGVLFIVLLMNLFLGKYRIESK
                     TLLAMLASFMIYITMETSSMSILLLIIVLCVYWPRGEEKLHEQV"
     gene            complement(1177..2265)
                     /gene="wecB"
                     /locus_tag="VXD58_001856"
     CDS             complement(1177..2265)
                     /gene="wecB"
                     /locus_tag="VXD58_001856"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV0676502.1"
                     /translation="MKKVMLVFGTRPEAIKMCPLVNELKQNDSIKTLVCVTGQHKEML
                     EQVLEVFKVVPDYDLGIMKANQTLFTITTSILDKIQAVLEKEKPDLVLVHGDTTTTFA
                     TALAAFYMGIKVGHVEAGLRTYNLQSPFPEEFNRQTTSIIADYHFAPTEVAKENLLRE
                     DRKNIFVTGNTVIDALKTTVQDNYHHPILEWAKGSKLIMLTAHRRENLGKPMENMFNA
                     VNRILNEFEDVKVVYPIHKNPKVRELASKVFGDNERMKIIEPLEVIDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVVAGTLKLVGTEEENIYSNFKLLLEDET
                     EYNKMSKASNPYGDGMTCKRIVDIILEV"
     gene            complement(2262..3347)
                     /locus_tag="VXD58_001857"
     CDS             complement(2262..3347)
                     /locus_tag="VXD58_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0676503.1"
                     /translation="MKSENQKNNILHISRTMDIGGAERIVYQLSSDLKDEFDSVHVAS
                     TGGLWESKLLSKGVQHHKILDVDSKNPLTMVKILSSFYRIIKNNDITIVHTHHRMAAF
                     YIRLLKIINPKLIHVYTAHNVFKNKLPLYKFSLKKAYCVAVSKAVNDNLKHDVGISDS
                     TVIYNGVVFEKTDKLVGEIVNYNGIKLGCIARLSEQKGLTYLIDAMSLVKHRNITLFI
                     VGDGELRDELENKVKQLNLQKSVIFLGYRKDIVECINSFDYLVSSSLYEGFGLNAIEA
                     FMKGKTMVSSDIPGINEVVNNKNGILVPVKDDVALARAIEKLATDKKLREKLAYQAKK
                     DYETKFNYSLFLDNYRRLYRKLMGESK"
     gene            complement(3322..4065)
                     /locus_tag="VXD58_001858"
     CDS             complement(3322..4065)
                     /locus_tag="VXD58_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV0676504.1"
                     /translation="MNERMQILGVTIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCHEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEILQDMLKVFKQRYPNLNVVGYRNGYFSPEDEKKIQEDIKGGKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQKLNLEWLFRVVN
                     EPKRLFKRYFIGNVTFIKRVLDEKRKSKK"
     gene            complement(4071..>4995)
                     /locus_tag="VXD58_001859"
     CDS             complement(4071..>4995)
                     /locus_tag="VXD58_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV0676505.1"
                     /translation="KLLESDELSWKLVAVSVLDKSDFQHDKIPVIEKEKIIEFATHEV
                     VDEVFVNLPGESYDIGEIISRFETMGIDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFS
                     TNFYKTSHVISKRILDICGATIGLILFAIASLVLVPLIRKDGGSAIFAQTRIGKNGRH
                     FTFYKFRSMRIDAEAIKEQLMDQNTMQGGMFKIDNDPRVTKIGRFIRKTSLDELPQFW
                     NVFIGDMSLVGTRPPTVDEYDQYTPEQKRRLSFKPGITGLWQVSGRSKITDFDDVVKL
                     DVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
BASE COUNT         1662 a          998 c          674 g         1661 t
ORIGIN      
        1 taacttgctc atgcaacttc tcctctccac gtggccaata tacacaaaga acaataatta
       61 gcagcaatat gctcatagat gaagtctcca ttgtaatata aatcataaat gaggctaaca
      121 tagccaacag tgtttttgat tctattctat attttccaag aaacagattc ataagaagca
      181 ctataaataa aacacctgga aatataccag ttcgagcaag tatatctaat aaggtattgt
      241 gtgctgaatt aacgataatc ccatttgtcc aagcattaaa attaacgttg aggcctcgtc
      301 caaaccaaaa attctcttca aaaatacgaa tcgctaattg ccaaatattg atacgtcctg
      361 aaaaagttgc atttttggaa aaaatcgctg taaaagttga taaaattgta ctttgtgaaa
      421 agattataga aagagaaaaa caaacatata cagccatact gactaaagaa tatagttttc
      481 ctatattttc ctttaatccc aatagacgta tgaaaataaa aagcccataa catataatcg
      541 ctgtactgga ttgtgttaaa aatgataaaa ttccgaacaa tcctaaaaaa ataatctcct
      601 ttagtgatat aaactcattc atttttttat ttaataagta aaaaccacct aatccaacaa
      661 tgatatacgg ggtagctatg ttctttcttc ctaaaaagta aagtctatta tctcctgtag
      721 ggtgcaaaga atcgaaaccg accggaaaaa gcaattgagt tattacttga acacacaaaa
      781 acaagataaa ggttaaaaag aggcttttta ttgttgtttt acaatccaac aataaaaagt
      841 gtttcaaaaa tgaatatagt cctaaaagga aaaactgacc tccaacatag ttaaggtaca
      901 acgtttggct ttgaaaagct gcaaatgctt gaaaaaataa aaatagaaca actagaatat
      961 caatcagtgt tactttatgc tgtaaaagaa ctttgggaat ataatcaaaa gttacaaata
     1021 gcttaactaa agtagaaagc aaagcaaata aagtcgccat tgatccaact gtaaaaaaac
     1081 taggtaaaaa atacgacagt aatacacaga ttatatatat ttttcttttt atcatcaatc
     1141 taatttattc tttcctcatc cataattgaa ttatttttat acttccagaa taatatcaac
     1201 aatccgttta caagtcattc catctccata agggttgcta gctttgctca tcttgttata
     1261 ttctgtttca tcttcaagaa gaagtttgaa gttgctatag atattctctt cttctgttcc
     1321 taccaacttc aacgtgccag caacgacccc ctcaggacgc tctgttgtat cacgcatcac
     1381 caagacaggt tttcctaaag aaggtgcttc ttcctgtaca cctccagaat ctgtcaaaat
     1441 catatagctt tgattcataa aattatgaaa atcaataacc tctaacggtt caatgatttt
     1501 cattcgttca ttatcgccaa aaactttact tgctaattct cgaaccttag gatttttatg
     1561 gatgggatat acaactttta catcttcgaa ttcgtttaaa attcgattaa cagcattaaa
     1621 catgttttcc atcggtttcc caagattttc tcgtcgatga gccgttagca tgattaactt
     1681 actacccttt gcccattcta aaattgggtg atggtagtta tcttgaacag tcgtcttcaa
     1741 agcatcaatg actgtatttc ccgtcacaaa gatatttttt ctgtcctctc ttagtagatt
     1801 ttccttcgca acttcagtcg gcgcaaaatg atagtcggca atgatagaag ttgtctggcg
     1861 attgaattcc tctggaaatg gactttggag attataggtt ctgagacctg cttcgacgtg
     1921 tcccactttg attcccatat aaaatgctgc taaagctgtc gcaaatgttg ttgtagtatc
     1981 tccatgaaca agaacaagat ctggtttttc tttctctaga accgcctgaa ttttatccaa
     2041 gatactagtc gtaatggtaa atagggtctg atttgctttc atgataccca aatcataatc
     2101 tgggacaacc ttgaacacct ctaaaacctg ctctaacatt tccttatgtt gaccagttac
     2161 acacacaagt gtcttgattg aatcattttg tttcagttca tttactaaag gacacatttt
     2221 aatagcttct ggacgtgtcc caaaaaccaa catgactttt ttcattttga ttctcccatt
     2281 aattttctat aaagtctccg ataattatcc aaaaacaagg aataattaaa ttttgtctca
     2341 taatcttttt ttgcttgata agcgagtttt tctctaagtt ttttgtcagt cgcaagcttt
     2401 tcaatcgctc tcgctaaggc aacatcatcc ttaacaggaa caagaatccc atttttgtta
     2461 ttgactactt catttattcc tgggatatct gatgaaacca tcgttttccc cttcataaat
     2521 gcttcaattg catttagacc aaacccctca tataaagatg atgacactaa gtaatcaaaa
     2581 ctattgatac attcaacaat atcttttcga taacctaaaa atatcactga tttttgaaga
     2641 tttaattgtt tcaccttgtt ttcaagctca tctcgaagct ctccatctcc gacaatgaac
     2701 aacgtaatat tcctgtgttt cactaaagac atcgcatcaa ttaggtaggt caatcctttt
     2761 tgctctgata acctagcaat acaccccaat ttgattccgt tatagtttac aatttcacca
     2821 actagcttat cagttttttc aaaaacaacc ccattataga ttactgtact atcactgatt
     2881 cctacatcat gtttcaaatt atcattaacc gctttactta cagctacaca atacgctttt
     2941 tttaaggaaa atttgtataa tggtaattta tttttaaata cattatgtgc agtgtaaaca
     3001 tgtatcaact taggattaat gattttcaac aagcgaatat aaaaggcagc catccgatga
     3061 tgagtgtgaa caatggtaat atcattattt ttgataattc tataaaaact tgaaaggatc
     3121 tttaccatag ttaggggatt cttactgtca acatctaaaa ttttatgatg ttgaactcct
     3181 tttgataaaa gtttgctctc ccatagtcca cctgttgaag cgacatggac actatcaaat
     3241 tcatctttca aatcagaact caattgatat acaatacgtt ctgcaccacc aatatccatt
     3301 gtacgagaaa tatgtagaat attatttttt tgattttcgc ttttcatcta aaactcgctt
     3361 aataaaagta acattcccta taaaataacg cttaaaaagg cgtttgggtt cattaactac
     3421 acggaacaac cattccagat tcaatttttg catccataat ggagctcgtt taatgtgtcc
     3481 tgataataca tcaaaactgc caccaacacc cataaagaca gcattaatcc cattatccat
     3541 gaatgattgg atgatatact cctttttagg agaagtaatc cctacaaata caaaatctgg
     3601 ttttcctcct ttaatatctt cctgaatctt tttttcatct tcaggtgaaa aatatccgtt
     3661 tctatagcca acaacattaa gattaggata tctttgttta aaaactttta gcatatcttg
     3721 taaaatttct tctttagctc caaaaaagta aactgaatat ccttttttat ttgacaactc
     3781 taaaagacat tgcatcaaat caatacccgc tactcgttca ggaacaggcg ttcctaaaaa
     3841 cttacttgca agaacaactg atgctccatc cgcattaatg attcctgatt cattaacgat
     3901 ttttttgatt ttctcatctt catggcactg attaatttta tctgcgttaa ctcccatcaa
     3961 gtgcaaagga tgcttcttta aaacatactg ttcaacagca tccactgttt ctttcatcgt
     4021 taatggatct atagtaactc ctaatatttg cattctctca ttcatatgaa ttacttcgct
     4081 ccatctctca taaatacaac tttaacagtt ttaagcaaaa tttcaatatc tttccagatt
     4141 gtccaattat caatataagc cacatctaat tttacaacat cgtcaaaatc ggttatttta
     4201 ctacggccgc taacctgcca taaacctgta ataccaggtt taaagctgag tcgacgtttc
     4261 tgttctggag tatactgatc atactcgtct actgtaggtg gacgtgttcc caccaaactc
     4321 atatctccta taaagacatt ccaaaactgc ggcaattcat ctaaactagt tttacgaata
     4381 aagcgaccaa tttttgtaac acgaggatca ttgtctatct taaacatacc accttgcatc
     4441 gtattttgat ccatcaactg ttctttgata gcttcagcat cgattcgcat cgaacggaat
     4501 ttataaaagg taaaatgtcg accatttttc cctatacgag tttgagcaaa aatagctgat
     4561 ccgccatctt tacgaatcaa tggaactaaa actagactag ctatagcaaa aagaataagg
     4621 ccaatagtgg caccacaaat atcgagaatt ctctttgaaa tcacatgact agttttataa
     4681 aaatttgtag agaaagtgac tacattcaat cctaccatct catgaatttg tttattgcga
     4741 cccaaattct tatcaaatgc tttaagattt acagttacat ctatccccat tgtctcaaac
     4801 ctagagataa tttctccaat atcgtagctc tctcctggaa gattgacaaa cacctcatcc
     4861 acaacttcat gcgttgcaaa ttcaataatt ttttcctttt caattacagg tattttatca
     4921 tgttgaaaat cagatttatc caaaacactt actgctacca atttccatga aagttcatca
     4981 gattctagca attta
//
LOCUS       DAVLFB010000109         4958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLFB010000109 DAVLFB010000000
VERSION     DAVLFB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.109"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(62..1885)
                     /gene="dnaK"
                     /locus_tag="VXD58_001860"
     CDS             complement(62..1885)
                     /gene="dnaK"
                     /locus_tag="VXD58_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV0676506.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2365..2889)
                     /gene="grpE"
                     /locus_tag="VXD58_001861"
     CDS             complement(2365..2889)
                     /gene="grpE"
                     /locus_tag="VXD58_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV0676507.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(2916..3950)
                     /gene="hrcA"
                     /locus_tag="VXD58_001862"
     CDS             complement(2916..3950)
                     /gene="hrcA"
                     /locus_tag="VXD58_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV0676508.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4114..4623)
                     /locus_tag="VXD58_001863"
     CDS             complement(4114..4623)
                     /locus_tag="VXD58_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV0676509.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
BASE COUNT         1357 a         1100 c          929 g         1572 t
ORIGIN      
        1 acaaaaatca ttataaactt ttcgtgtatt ttttagatac tcttcatcca atacactcat
       61 cttacttttc cgtaaactct ccgtctacga cgtcatcgcc tgcgttccct gttgcttgtg
      121 cgccttctgc tccttcttga gcttgttgcg ctgctgcggc ttgttcgtag agtttaacag
      181 caagtccttg agctttttcg ttcaatgctt caagttttgt tttcatgtcg tccaagttgt
      241 tgtcttcttg agctttctta aggtcatcaa gggcagcttg ggcagcgtca cgttctgcgt
      301 cgaagccttt accttcagtt tccttgattg tcttttcagt cgcaaagatt gcttggtcta
      361 cttcattacg aaggtctact tcttctttac gtttcttatc ggcttcagcg tttgcttctg
      421 catctttcat catgcggtcg atttcttcgt cagtcaaacc tgagttcgat tggatgacaa
      481 tagtttgttc tttttgagtt ccaaggtctt tggccttaac agacacgata ccgttcttgt
      541 cgatgtcaaa tgttacttcg atttgaggaa ttccacgagg tgcagctggg atatcagtca
      601 attggaagcg tccaagagtc ttgttatctg ctgccattgg gcgttcacct tgaagaacgt
      661 ggatatcaac ggctggttgg ttgtctgctg ctgttgagaa gacttgtgat ttagatgttg
      721 ggattgtagt gttgcgatcg ataagttttg taaatactcc acccattgtt tcgataccaa
      781 gtgacaatgg cgttacatca agaaggacaa cgtccttgac atcaccagta atcacaccac
      841 cttggatagc cgcacccata gcaactactt catcagggtt tactgatttg tttggttctt
      901 taccagtttc agctttaaca gcttcaacaa cggcagggat acgagttgaa ccaccaacaa
      961 ggataacttc gtcgatttct gacaagctca aacctgcatc tgaaagggct tgacgaactg
     1021 gaacttttgt acgttcaaca aggtcacgag tcaaatcgtc aaatttcgca cgagtcaaag
     1081 tcatttccaa gtgaagaggt ccagcctcac ctgcagtgat aaatggcaag ctgatttgtg
     1141 ttgaagttac accagaaagg tctttcttcg ctttttcagc cgcatctttc aaacgttgca
     1201 ttgccatctt gtcagtagac aagtcgatac cgttttcttt cttgaattct gctaccaagt
     1261 ggtcaatgat tttttggtca aagtcgtcac caccaagttt gttgtcccct gcagttgaca
     1321 atacgtcgaa gacaccgtca cccaattcaa ggatagagac gtcgaatgta ccaccaccaa
     1381 ggtcaaatac caagattttt tcttctttgt cagtcttgtc caaaccataa gcaagagctg
     1441 ctgcagttgg ttcgttaaca atacgttcta cttcaagacc agcaatttta ccagcgtctt
     1501 ttgttgcttg acgttgagcg tcgttgaagt aagccggaac tgtgataaca gctttggtta
     1561 ctttctcacc aaggtagtct tcagcgtagc ctttcaagta ttgaaggatc atagctgaga
     1621 tttcttgtgg agtgtattct tttccatttg cagaaacttt ttcagaagtt cccatcttag
     1681 atttgataga gataactgta tctgggtttg taactgcttg acgttttgca gcatcaccaa
     1741 cgatgatttc tccgtttttg aatgagacta cagatggagt tgtgcggttt ccttctgggt
     1801 ttgcgatgat tttgctttca gttccttcaa gaactgcaac tgctgagttt gttgtaccta
     1861 agtcaatacc gataatttta gacatgtgtt tttctccttg ttagttaatt ttctattttt
     1921 ctttttgata ctcttcttaa gtggcgacgc cgtcagagct tgacttcgtc agtctctttt
     1981 actaaacttt gagcctaagg tctcaaaatt tgcgcaaata gcgccacggc gaagagtatc
     2041 gtctttggtt cgcttcgctc actattacaa agcaaaacat attttatctt tcttcatagt
     2101 ttattgtcgt ttcggacaag ttttctatta tgtaaattgc gacacgagga gtcgaaatcg
     2161 attttatttc gacgacgagt tagtaaggaa gctaggcaaa cgccatagca attgccgttt
     2221 tctgacgagt tgctttagct accgtcagaa ttgccgaatc gaacacgccc taagctctga
     2281 gtaaaaaaga tagattttct tgtgttcatc gaacacttcg tcaatctcct atttttactg
     2341 cgagcttttt acgggctttg tatcttagtt atacaccact accattgctg ggcgtaggat
     2401 gcggtcatgg agtttgtagc ctttttgaaa gacttgggcg atggtatcta ctgggtgttc
     2461 atcgtctgct gggagagttt ggatggccat atggtagtta tggtcaaatt cgccatctgc
     2521 tgcgatttct tcaattcctt cttctttcaa agcgtgaatc aagctttctt gcaccatccc
     2581 caagcccttc ttcacatcat ctgtcaaacc ttcaactgca agtgcacgct caaggttgtc
     2641 aagagatggt aagattgctt ttgccaagtc ctggctacga taacgttgca agttttgacg
     2701 ttcttcattg gcacggcgtt ggatattttg catttctgca tgagcgcgaa gatatttgtt
     2761 ttcgaactca tctgcacgtt catttgccaa gtccaactca gacttttcag gagttgtttc
     2821 ttcagctgtt tccacaactt cctcttcttg aacttcttct acttcttcat tttttatatc
     2881 ttgggccatg ttcgcctcct ttaatgattt caatcttaat gtacttcgta atgattactg
     2941 ctgaggtagc ggtaaaaatc tgtcaacttc atggtcaaaa cacggttgac cacattgatt
     3001 tgattgatta gctgttggta atccagatta actggaccga taatggctag aattccaact
     3061 ccccgataag gaatgaggaa cttactacta atcaccgcta ggtcagctaa acaggactct
     3121 tgaccgtctg caacacgaac attttgcatc tgatcctcac gcaacccctc acgaatctcc
     3181 aaggccacct tttgcggttg gtcaaagaac tgataggctg ctagattggc aaaattcaag
     3241 agattgacct tgcccgccat cacaatgttt tcgttgaaca tttccttaaa gatgtgttca
     3301 aagagatcga tgacattatc cgttgttgta aagtaacgct ggataatctg cggaatctcc
     3361 gtccgaatct tgtagtgaat atctaaaacg gtgtgaccga ggaaacgttc ctgaatgatg
     3421 ctcttcagtt tcagcaaatc ctcctgcaag aagttccttg gaatcagaaa ctgactagta
     3481 accgttcgcg actcgtctag ggtaaatacc gccaaggctg tatgttgccc caaaacaacg
     3541 atatcaaagg ctgtcaaacg ttgcctgctc ggctcaacat ccagtgccac taccgtacag
     3601 ccactcaggt ctgttagcaa gttagcagcc tcttgcagaa tatcctccaa tttgaagaat
     3661 tcctgatcaa aggctttgac aatctcatat acctcatttt cagccagtcg gtcaaaatcc
     3721 agtgagtgtt tcacatagta ctgaaaacca gcaacacttg gcatccgacc gcttgaagta
     3781 tgagccttct caagcaaccc ttgtttttct agttccgcca tgtcattacg aatggttgca
     3841 ctgctagagt taatagactc ttgcaaggct tttgatccga caggttcgtg cgttttggta
     3901 aagatgtcaa taatcagatt taaaatatcc tgctgacgct ctgtaaccat ctcatcactc
     3961 ctctctgtct gatcaattag cactcaatgc atccaagtgc taacttatga ttctattata
     4021 cacccttaga agagaatgtc aagacaaaaa ctcaaaaaat tagcactctt ttataaagag
     4081 tgctaaaaat caatctgaag acctagaacc ttgctattca tctactcggt atttcctttt
     4141 tagtctgaaa taaccatgaa ttagataaga aatcagaaaa gcatataaaa caaaaataag
     4201 aaagaaagac atagaaagat cctctctaaa caaactcata caaacaacaa gaccacctaa
     4261 gaaagtagct atacaggaac gaagtctaga tcgcataact tcccatttga gccccttact
     4321 catatagact tgatcctctg gaagtaaatc agaagacgat ttacgaagaa tcgaacaaga
     4381 acctgctcct atcaattccc aacctctatc tctaaaatct tgcagttcac gcttatgctc
     4441 tttaagaaat ctagaatcat agatacgata gataacatcg tccggttgac cttgctcaaa
     4501 atagaacaaa ccaaaacgac tagttctata cctccaacct ttcaaatgca tctcatgtaa
     4561 atattcttct ttcttgtcca aatcaacaat ggtgaaaatc cgaaattcta ctctgctatt
     4621 cattgtctta ccccaaaatt agaaaacatg cctagcgtta tttattagat aattctttcc
     4681 acttttgact caatctccaa aaaatataag aaatctgaat cgcaaaaact atcaataaaa
     4741 cccaatctat tatgaaaatc aaaaacactt cccaactgaa agaactacct ccagtgacaa
     4801 actttgagaa aaacggtagt agagctaaaa agagaagcaa aataggaagc atccgcattg
     4861 ttaaaatcct tttggcataa aaaaatcttt atttaaacta aaatattatg gcaaaattta
     4921 cgccagtttt tgaacggctg atgtagatat tttatact
//
LOCUS       DAVLFB010000110         4826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLFB010000110 DAVLFB010000000
VERSION     DAVLFB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.110"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(58..1068)
                     /locus_tag="VXD58_001864"
     CDS             complement(58..1068)
                     /locus_tag="VXD58_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEV0676510.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1217..2386
                     /locus_tag="VXD58_001865"
     CDS             1217..2386
                     /locus_tag="VXD58_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV0676511.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2383..3153
                     /gene="recO"
                     /locus_tag="VXD58_001866"
     CDS             2383..3153
                     /gene="recO"
                     /locus_tag="VXD58_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV0676512.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3150..4142
                     /gene="plsX"
                     /locus_tag="VXD58_001867"
     CDS             3150..4142
                     /gene="plsX"
                     /locus_tag="VXD58_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV0676513.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4148..4381
                     /locus_tag="VXD58_001868"
     CDS             4148..4381
                     /locus_tag="VXD58_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV0676514.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4427..4717
                     /locus_tag="VXD58_001869"
                     /pseudo
     CDS             4427..4717
                     /locus_tag="VXD58_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1441 a          915 c         1208 g         1262 t
ORIGIN      
        1 aacatatacc tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtgtta
       61 gaaaatgccg ataagggtct gcataccaag agtggtgagg atgatggcaa tccagcagat
      121 ggctccaaga acaatggatt ttccactgga tttgaccata gcgaccagat tggttttgag
      181 accgatagca ctcatggcca tgacaataag gaatttagag agttgtttga gaggggtaaa
      241 gaaactactg gacacaccta gagaggtgag tagtgtagtg aggagagagg caaggataaa
      301 gtaaaggata aaaagtggga agactttttt cagttgtagg ctttgcttgt tttcttgttg
      361 gcgactttgc cagtaggata gaaagagcgt gatagggata atggccaaag tacgtgtgag
      421 tttaacaatg gttgcagact cgagggtatt gctttggtaa agactgtccc aagcgctggc
      481 ggcagccgtt acagaggaag tgtcgttgac cgcagtccct gcaaagaggg cgaagccttc
      541 attggataga tgaagccagg tgccgagggt tggaaagatg agcgcagcca agacattgaa
      601 gaaaaagata acggaaatgg cttgggctac ttccttttcc ttagcatgga taacaggcgc
      661 tgtcgctgca atggcagaac ccccacagat agaagaacct actccaacca aggtagccag
      721 ttttgtatcc agtgcaaaga aacgctggaa gaggtaggca ataatcagag ctattgagat
      781 agtggacagg atgacaggga gtgaagattg gccaactgcg aagacctgcg agatattgag
      841 accaaaacca agcaagacaa cggcatattg gagcaacttc ttggaactaa aggtcaaacc
      901 agcatccagt tgtttatagg acgagagaaa gggatgtagg agcatgccta tgaaaatggc
      961 aaaaacgggc gcgccaatca cagggaagaa tcctcctaag taccaagata ggatggaaat
     1021 gagaaggcag gccaagatac ctgctccatt ttttgataga aatgacataa aaacctccaa
     1081 ataaaatcta ttaccattat agacctataa acaggaaaag taaaatagaa agtggaaagc
     1141 tattctctaa tgtatttttg cggtcggggg gcttttgtag tataatagag atacgttttg
     1201 aaagtaggag gtatctatgg acttaactaa gcgctttaat aaacagttag ataaaattca
     1261 agtttcgttg attcgtcagt ttgaccaggt tatttcggag attcctgggg tcttgcgttt
     1321 gaccttgggg gaacctgatt ttacaacgcc agaccatgtc aaggaggcgg ccaagcgagc
     1381 gattgatcag aaccaatcct actatacagg gatgagtggt ctgctgactc tacgtcaggc
     1441 agccagtgac tttgttaagg aaaagtacca actggactat gctcctgaaa atgaaatctt
     1501 ggttacaatt ggggcgacag aggctttatc tgcgactttg acggctattt tggaagaggg
     1561 agacaaggta cttttgccag ctcctgctta tccaggctat gaaccgattg ttaacttagt
     1621 tggggcagaa attgttgaga ttgatacgac tgaaaatggt tttgtcttga ctcctgagat
     1681 gttggagaag gccattttgg agcagggtga taagctcaag gcggttattc tcaactatcc
     1741 agccaatccg acaggaatta cctacagtcg agagcagtta gaggccttgg cagctgtttt
     1801 acgcaagtac gaaatttttg ttgtctgtga tgaggtttac tcagaattga cctacacagg
     1861 cgaagcccat gtgtctctag gaacgatgtt gagagaccag gctattatta tcaatggttt
     1921 gtctaaatcg catgccatga caggttggcg tttggggctg attttcgctc ctgcgacctt
     1981 cacagcccag ttaatcaaga gtcaccagta cttggtcact gccgcaaata ccatggcgca
     2041 acatgctgca gtagaagcct tgacggctgg taaaaacgat gcggagccca tgaagaagga
     2101 atatatccaa cgtcgggact atatcatcga aaaaatgact gctcttggtt ttgagattat
     2161 caaaccagac ggtgccttct atatttttgc taaaattcca gcgggctaca atcaagactc
     2221 ctttgctttt ctgaaggatt ttgctcagaa gaaggccgtt gcctttatcc ctggtgcagc
     2281 ctttggacgt tacggggaag gctacgtccg cctatcttat gcagccagca tggagactat
     2341 caaagaagcc atgaaacgac ttgaggagta catgagagaa gcatgattca gtctatcacg
     2401 agtcaaggct tggtgcttta caatcgcaat tttcgtgagg atgacaagct cgtcaaaatt
     2461 tttacagagc aggttggcaa acgcatgttt tttgtcaaac acgctggtca gtctaagctg
     2521 gcgcctgtta ttcagccctt ggtgctggca cgatttctct tgcgaatcaa tgatgacgga
     2581 ctcagttaca tcgaagacta tcatgaggtc atgacttttc ccaagattaa tagtgacctc
     2641 tttgtcatgg cctatgcgac ctatgtggca gctcttgcag atgctagttt gcaggacaat
     2701 cagcaggatg ctcccttgtt tgcttttttg caaaagactt tggagttgat ggaagcaggc
     2761 ttggattatc aggttttgac caatattttt gaaattcaaa ttttgactcg atttggaatc
     2821 agcctcaatt ttaatgagtg tgtcttctgc catcgggttg gtcaggcttt tgacttttct
     2881 ttcaaatatg gagcctgcct ctgtccagag cattatcatg aggataagag acgttgtcat
     2941 ctcaatccca atatccccta tctgctcaat caatttcaag ctattgattt tgagactttg
     3001 gagaccattt cgctcaagcc tggaatcaag caagagctac gccaatttat ggatcaacta
     3061 tatgaagagt acgttgggat tcacctaaaa tcaaagaaat ttattgattc cctagcagac
     3121 tggggacaat tactaaaaga ggaaaagaaa tgaaaaaaat cgcagtagat gccatggggg
     3181 gcgattacgc acctcaggcc attgttgagg gtgtcaatca agccctatct gacttttcag
     3241 atatcgaggt tcaactttac ggagatgaag ctaaaatcaa gcaatatctg acagcgacag
     3301 agcgcgtcag cattatccat acggatgaga agattgattc ggatgatgaa cctacgagag
     3361 ctattaggaa taagaaaaat gccagtatgg tattggcagc caaggctgtc aaagatggtg
     3421 aagcagacgc tgtcctttcg gctgggaata caggtgcctt gttggcagca ggattcttca
     3481 tcgtgggtcg tatcaagaat atcgaccgtc ctggactcat gtctaccttg cctaccgttg
     3541 atggaaaagg ttttgacatg ctagaccttg gtgccaatgc agaaaataca gcccagcacc
     3601 tccatcaata tgcggttcta ggttccttct atgctaaaaa tgtccgtggc attgcgcaac
     3661 cacgcgttgg tttgctcaac aacggaacag agagtagcaa gggcgacccg cttcgtaagg
     3721 aaacttatga attactggcg gctgatgaaa gtttgaactt tatcggaaac gtggaagcgc
     3781 gtgatttgat gaatggcgtt gcagatgttg ttgtggcaga tggtttcacg ggaaacgctg
     3841 tgctcaaatc catcgaaggg acagctatgg gaatcatggg cttgctcaag acagctatta
     3901 caggtggtgg tcttcgagcg aaactaggtg ccctccttct caaggacagc ctcagtggtt
     3961 tgaaaaaaca gctcaattat tcagatgttg gtggagcggt cttgtttggt gttaaggcac
     4021 ctgttgtcaa gactcatggc tcaagcgatg ccaaggctgt ttatagtacg attcgtcaga
     4081 tccgtaccat gctagaaaca gacgtggttg cccagactgc gcgtgaattt tcaggagaat
     4141 aaaagagatg acagaaaaag aaatttttga ccgtattgtg accattatcc aagagcgaca
     4201 gggagaggac tttgtcgtga cagaatcctt gagtctgaaa gacgatttgg atgcggattc
     4261 tgttgacttg atggagttta tcttgactct ggaagatgaa tttagtatcg aaatcagcga
     4321 tgaagaaatt gaccaactcc aaaacgtagg agatgtggtt aaaatcattc aaggaaaata
     4381 gtaatcggag ttccaagtca acggaagtag atggttttta gaaatgagaa atatcggaca
     4441 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcttcaagc
     4501 acaaactcca cgtaaatcca gcaaactcaa gccgctaaca gctgaagata aagcctataa
     4561 ccatgcgcta tccaaggaga gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt
     4621 taaaatgatt tcaacaacct atcgaaatca tcgtaaacac ttcggattac gaatgaattt
     4681 gattgctggc attatcaatc atgaactagg attctagttt tgcaggaagt ctaatagtaa
     4741 aaaagtgatt agaaaacatc ttttttaaaa atagagatga ttttgaaaca aaaaagctaa
     4801 ttcaagacgt ttcgatgcca attcaa
//
LOCUS       DAVLFB010000111         4792 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLFB010000111 DAVLFB010000000
VERSION     DAVLFB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.111"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..202
                     /locus_tag="VXD58_001870"
     CDS             <1..202
                     /locus_tag="VXD58_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV0676515.1"
                     /translation="DDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEI
                     NNYLKKYECYSPLNNLELDDFE"
     gene            complement(368..439)
                     /locus_tag="VXD58_001871"
     tRNA            complement(368..439)
                     /locus_tag="VXD58_001871"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(404..406),aa:Arg,seq:cct)
     gene            complement(481..828)
                     /gene="rplS"
                     /locus_tag="VXD58_001872"
     CDS             complement(481..828)
                     /gene="rplS"
                     /locus_tag="VXD58_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV0676516.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(947..1276)
                     /gene="crcB"
                     /locus_tag="VXD58_001873"
     CDS             complement(947..1276)
                     /gene="crcB"
                     /locus_tag="VXD58_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0676517.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1270..1644)
                     /gene="crcB"
                     /locus_tag="VXD58_001874"
     CDS             complement(1270..1644)
                     /gene="crcB"
                     /locus_tag="VXD58_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV0676518.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(1641..1907)
                     /locus_tag="VXD58_001875"
     CDS             complement(1641..1907)
                     /locus_tag="VXD58_001875"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV0676519.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2023..2466)
                     /locus_tag="VXD58_001876"
     CDS             complement(2023..2466)
                     /locus_tag="VXD58_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV0676520.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2570..3505
                     /locus_tag="VXD58_001877"
     CDS             2570..3505
                     /locus_tag="VXD58_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV0676521.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3559..3843
                     /locus_tag="VXD58_001878"
     CDS             3559..3843
                     /locus_tag="VXD58_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV0676522.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3900..4118)
                     /locus_tag="VXD58_001879"
     CDS             complement(3900..4118)
                     /locus_tag="VXD58_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676523.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4115..4689)
                     /locus_tag="VXD58_001880"
                     /pseudo
     CDS             complement(4115..4689)
                     /locus_tag="VXD58_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1496 a         1029 c          844 g         1423 t
ORIGIN      
        1 tgatgacgat tatgattttg cttttgatga agcattattt cgcttctcaa ttttgaatgc
       61 aaatcatttt ttatctaagg aagatattga ctatttaaga gttaatttac ctcgttccac
      121 tgctgaagaa ataaacaatt acttaaagaa atatgaatgt tatagtcctt taaataattt
      181 agaacttgac gattttgaat aaattgacta tacaaacatt tatatactcg atatagtctc
      241 aattttatct gatgattgcc caaatttttc aataataaaa cgcataatat tatggagaca
      301 atcccctata ttatgcgttc ttttaatatc aaagactttt tgacaaactt cttcgatatc
      361 taattactgc cccctgcagg aatcgaacct gcaactactc cttaggaggg agttgttata
      421 tccattgaac taagggagct agataaaaac tctgctgaaa agcagagttt tttagtcgaa
      481 ttaacgacgg atttctttga tacgagctgc cttaccttga agagcacgca agtagtacaa
      541 tttcgcacga cgtactttac cgtaacgaac aacttcgatt ttttcaacac gtggagtgtg
      601 gattgggaag atacgctcaa cacctacacc gttagagatt ttacgaactg tgtagttttc
      661 tgagattcca gcacctttac gtgcgataac aacaccttca aaaatctgga tacgttcacg
      721 gttaccttcg acaactttcg catgtacacg aacagtgtca ccaggacgga atgatgggat
      781 atctgtacga agttgacctt cagtcaagct ttggattaat ggattcattt tattctccta
      841 tctttgtcaa tcttgaggaa tcttcctcag cggataaact gtatttttgt gcgtccatta
      901 cacacaagat atagtttacc aaatttctaa aaaaaagtaa agaaatttat agcaaaattc
      961 ctaaaaaaat cgcaaccaaa ccacctatgt aagtcaaagt caggtaagaa taaaagacct
     1021 tcttatcact tagcagtctt tgaagttcgt cattcaaggt cgaaaaagtt gttaaaccgc
     1081 cacaaaatcc tgttgctaga atagcatata cttccttaga ctctacatga ttgtagaata
     1141 ctccaattaa aaaacaacct aaaagattag ctatgagcgt tccgagtggc aatttagaag
     1201 cttgattata gcgagaaaag aaataccgta cgagtgctcc caagccacag gcgattgcaa
     1261 gatagacgat taccatttct tcctccccaa atagtaagct aaaagcaggc ctccaccgat
     1321 actcaaaagc aaatacagta tcaaactaag ataacgccct gtatcaagca gcttcacagt
     1381 atcaagcatt aggctggaaa aagttgttaa acctccgcaa aaacccgtcc ccagtgctaa
     1441 aatcaagccc ttactagtcc ccttatagac cagatagccc ttgacaagat aaatcaagca
     1501 gaaaattccc agatagttga caaagagagt gccccaaggg aaatctggac tggctggtaa
     1561 ccaagtggaa actaggtagc ggacaagtcc gcccaacata gcagctagaa aaatccctag
     1621 cggataaaat tgttcttttt tcatttgata ttttgatcct gataatcacg cgaacgtttg
     1681 agtatgtccg aaaaagtcgc gacaatagtc tcctgatagc gcttgtcctc tacacgactt
     1741 ctaacctttt caaaaataac tgcttctctc ttagtatcta aaatcggttt ccctgaagcc
     1801 ttcttataag cgacaactcc ttcaactaag tgcattcgtt cttctaacag tttgacaatt
     1861 tggtcgtcga tttgatcgat ttcttgacga ataatatcta aatccataca gtctcctcct
     1921 ttatttgaat tattgtatca aaaagccatc aattaggcta gtaaaatccc aactctcgat
     1981 aagaaaaatg cactgattga ttacatcagc gcacttttat gcttatccta ctttagcagc
     2041 caattcttct gcgaattgtt ccaagcgttc aatatcttct tcctcggcag aaagatcaac
     2101 tttaacacac tctgaacctt tttctgctcc tgttgacaca aagacacggt caaagtcatc
     2161 aacagcctta caaaattcat cgtagaaggt atctcctgag ccgaccactc cgtagatttt
     2221 gccattcaag ttgagatctg ctaggtcttc gtagaagtcc atcatctcat ctggcaattc
     2281 accatcacca taagtatagg tcgcaacgat agcgatgtct gcttccaaga agtctgaagc
     2341 gtcaacagtt gtacattcat caacatcaac atccaagccc aagtcacgta atttgtctgc
     2401 tacaatatct gcaatttctt cggtattacc ggtcatactg gcaaatacaa tttttgctaa
     2461 tgccatatcg tcctcctcaa tttatctttc tccattatat cacatctttt tcattttaaa
     2521 aataaaaatt cttgtgctac aatgaaatga gaaagaattg aggttattta tggagatttg
     2581 ccaacaaatt ttagaaaaaa ttaaagaata cgacaccatt atcattcatc gtcatatgaa
     2641 accagaccct gatgccttgg gaagtcaggt gggattgaaa gccttgctag aacatcattt
     2701 cccagaaaaa accatcaaag ccgtcggttt tgatgaacca actcttactt ggatggctga
     2761 gatggatctt gttgaagata gagcctacca aggcgcactt gtcatcgtct gtgatacagc
     2821 taatactgct cgtatcgatg ataagcgcta tagtcaaggt gattttctca ttaagattga
     2881 ccaccatcca aatgatgatg tatacggtga cctgtcttgg gtcgatacta gttcaagtag
     2941 cgctagcgag atgattaccc tatttgccca aacaacccaa ctagccttgg cagatcgcga
     3001 tgctgagttg ctctttgcag gaattgtcgg tgatacaggt cgcttcctct acccttctac
     3061 cactgcacgg actcttcgcc tggctgctta tttgagagaa cataactttg actttgcggc
     3121 tctcactcgc aaaatggaca ctatgagcta caaaattgct aaactgcaag gctacatcta
     3181 cgaccatctg gaagtggatg aaaatggtgc tgctcgcgtt atcctgagtc agaaaatctt
     3241 gaaacaatac aatataaccg atgctgaaac tgcggccatt gtaggtgcac ctggacgcat
     3301 tgacagagtg agtctctggg gaatttttgt cgaacaggct gatggccact accgagttcg
     3361 cttacgcagt aaagtccatc ctatcaatga aattgccaag gagcatgatg gtggaggcca
     3421 ccctctagca agtggtgcta attcctatag cctagaagaa aacgaaatca tctaccaaaa
     3481 gttaaaaaac ttgcttaaaa actgataaaa tacttgccaa acttttcaga atctgataga
     3541 ctagtatagt aacaatctat ggctcgcaaa gagaccatgg cagaaaggaa atattgcaaa
     3601 atgaaaaaag atatccatcc agaatatcgc ccagttgtct tcatggacac aactactggt
     3661 taccaattcc ttagcggttc aacaaaacgc tctaacgaaa cagttgagtt cgaaggcgaa
     3721 acttacccat tgatccgtgt ggaaatttca tcagactcac acccattcta cactggacgt
     3781 caaaagttca ctcaagcaga tggacgcgtg gatcgtttca acaaaaaata cggtctcaaa
     3841 taatgataag agaacagttt tggctgttct tttttgtttc ttgaaatcaa ctgctgtttt
     3901 catgttccag actcatctgt aggttcgatt tccatgctac taggcaggaa ggaaatagct
     3961 gtttcaacac gtccataatg agctatacta ttgtcacgaa ccacactttc attgatggtc
     4021 caagtggaat tcattttctt aaaagcttct cggacttttt ccaaatcttt ggaggcaatg
     4081 gcctgctcta aggtttcaaa acgaggactt atactcatct gctttcaaaa agcattctag
     4141 tccatctccg attaccgatg gactttatca cctccttctc cagtccttgt atgacatctt
     4201 gaagttgatt catgacatct tccaaagttc gaaaggcttt attcttaaat ccacgtttac
     4261 gaatctcttt ccacacttgt tcaatgggtt catctctggt gtgtatggag gaataaaggt
     4321 aaaatcaata ttagtcggaa tatttaaggt acttgattta tgccatatag cattgtccat
     4381 aacgagtaaa aggataagct tgtgaaagct cttctaaaaa ggcgttcatc cacactcctt
     4441 tttataaacc tgaaataagg catcaattgt aacaaattct cctgcctctg tagccttcaa
     4501 atgacgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt tacgaccacc
     4561 acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttta cgtttcgcca
     4621 aatcgttgtt tgattacagt ctaaaagctc tataatctct ttataagatt tgcccatcag
     4681 acgaaatata gtagattgaa actagaatag tacacctcta cttctaaaac attgttagaa
     4741 atcaatttgt cctgttcttg tttcatttta ctatagaacg atttgaaggc gt
//
LOCUS       DAVLFB010000112         4626 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLFB010000112 DAVLFB010000000
VERSION     DAVLFB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.112"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(101..174)
                     /locus_tag="VXD58_001881"
     tRNA            complement(101..174)
                     /locus_tag="VXD58_001881"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Arg,seq:tct)
     gene            complement(218..697)
                     /gene="rlmH"
                     /locus_tag="VXD58_001882"
     CDS             complement(218..697)
                     /gene="rlmH"
                     /locus_tag="VXD58_001882"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV0676524.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            880..2061
                     /locus_tag="VXD58_001883"
     CDS             880..2061
                     /locus_tag="VXD58_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV0676525.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2119..2877
                     /locus_tag="VXD58_001884"
     CDS             2119..2877
                     /locus_tag="VXD58_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV0676526.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            3090..4451
                     /gene="dnaA"
                     /locus_tag="VXD58_001885"
     CDS             3090..4451
                     /gene="dnaA"
                     /locus_tag="VXD58_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV0676527.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1622 a          816 c          784 g         1404 t
ORIGIN      
        1 atttcacttt agataaaatt ctctcaactg tcattttttt ctcccaaagt gtactttttt
       61 aagaaaaaag ccgggaaaat tcccagcttt gctattatat tgatcccagc aggattcgaa
      121 cctgcgaccg ttcgcttaga aggcgaatgc tctatccagc tgagctatga gacctaacac
      181 aattattcta ccaaaaattc aattaaaagt caattttcta tttatggtag gggaatccct
      241 gctgaatcgt aaaagcgcga tagatttgtt caacaagaac tagtctcatt aactgatgag
      301 gcaaggttag gcgaccaaaa ctgacagaaa gattggctct attttttaca gatgatgaca
      361 atcctaaact tcccccaata ataaaagtaa gagtagaaaa tccttttata gaagtttctt
      421 ctaactgctt actaaattct tctgagaaga aagttttccc ttcaatggct aacacaataa
      481 cgaaatcacg gtcagcaatt tttgataaaa ttctctgacc ttctatttct aaaatctttt
      541 gattttctga ttcactggcc ttatctggtg ttttttcatc tgataactca atcatttcaa
      601 acttagcaaa tctagaaatt cgttttgaat actctgcgat accatctttt aaatactttt
      661 ctttcagttt cccaactgtt acaactttaa ttttcatgac tctattctaa catattctct
      721 attttttcac atcttattca caaaataaaa aatagatttc aattaagaaa atcacagttt
      781 caaaagagtt atccacagtt tgtgtaaaac ttttgtgttt aagttataat taagctagtc
      841 agtttatact ttcagtaatt caaacatatg gaggcaaata tgaaacatct aaaaacattt
      901 tacaaaaaat ggtttcaatt attagtcgtt atcgtcatta gcttttttag tggagccttg
      961 ggtagttttt caataactca actaactcaa aaaagtagtg taaacaactc taacaataat
     1021 agtactatta cacaaactgc ctataagaac gaaaattcaa caacacaggc tgttaacaaa
     1081 gtaaaagatg ctgttgtttc tgttattact tattcggcaa acagacaaaa tagcgtattt
     1141 ggcaatgatg atactgacac agattctcag cgaatctcta gtgaaggatc tggagttatt
     1201 tataaaaaga atgataaaga agcttacatc gtcaccaaca atcacgttat aaatggcgcc
     1261 agcaaagtag atattcgatt gtcagatggg actaaagtac ctggagaaat tgtcggagct
     1321 gacactttct ctgatattgc tgtcgtaaaa atctcttcag aaaaagtgac aacagtagct
     1381 gagtttggtg attctagtaa gttaactgta ggagaaactg ctattgccat cggtagcccg
     1441 ttaggttctg aatatgcaaa tactgtcact caaggtatcg tatccagtct caatagaaat
     1501 gtatccttaa aatcggaaga tggacaagct atttctacaa aagccatcca aactgatact
     1561 gctattaacc caggtaactc tggcggccca ctgatcaata ttcaagggca ggttatcgga
     1621 attacctcaa gtaaaattgc tacaaatgga ggaacatctg tagaaggtct tggtttcgca
     1681 attcctgcaa atgatgctat caatattatt gaacagttag aaaaaaacgg aaaagtgacg
     1741 cgtccagctt tgggaatcca gatggttaat ttatctaatg tgagtacaag cgacatcaga
     1801 agactcaata ttccaagtaa tgttacatct ggtgtagttg ttcgttcggt acaaagtaat
     1861 atgcctgcca atggtcacct tgaaaaatac gatgtaatta caaaagtaga tgacaaagag
     1921 attgcttcat caacagactt acaaagtgct ctttacaacc attctatcgg agacaccatt
     1981 aagataacct actatcgtaa cgggaaagaa gaaactacct ctatcaaact taacaagagt
     2041 tcaggtgatt tagaatctta attgacatct atgtaaagaa agctttacat aagagaaaag
     2101 atgtgttagt gtagaatcat ggaaaaattt gaaatgattt ctatcacaga tatacaaaaa
     2161 aatccctatc aaccccgaaa agaatttgat agagaaaagc tagatgaact agcacagtct
     2221 atcaaagaaa atggggtcat tcaaccgatt attgttcgtc aatctcctgt tattggttat
     2281 gaaatccttg caggagagag acgctatcgg gcttcacttt tagctggtct acggtctatc
     2341 ccagctgttg ttaaacagat ttcagaccaa gagatgatgg tccagtccat tattgaaaat
     2401 ttacagagag aaaatttaaa cccaatagaa gaagcacgcg cctatgaatc tctcgtagag
     2461 aaaggattca cccatgctga aattgcagat aagatgggca agtctcgtcc atatatcagc
     2521 aactccattc gtttactttc cttgccagaa cagattctct cagaagtaga aaatggcaaa
     2581 ctatcacaag cccatgcgcg ttccctagtt gggttaaata aggaacaaca agactatttc
     2641 tttcaacgga ttatagaaga agatatttct gtaaggaagt tagaaactct tctgacagag
     2701 aaaaaacaaa agaaacagca aaaaactaat catttcatac aaaatgaaga aaaacagtta
     2761 agaaaactac tcggattaga tgtagaaatt aaactatcta aaaaagacag tggaaaaatc
     2821 attatttctt tttcaaatca agaagaatat agtagaatta tcaacagcct gaaataaggc
     2881 tgttctttta tttttttatc tcacaaggtt atccactatg tttttcgata aaaagcttaa
     2941 taaatcaata atttcttctt ttatccccaa cctgtggata aagtttggta acattgtgga
     3001 ttatttttca cagcttgtgg aaaattcttg ctatctatgg taaaatatct ctagtattaa
     3061 acttttaaat agtaaaggag gagaaaggat tgaaagaaaa acaattttgg aatcgtatat
     3121 tagaatttgc acaagaaaga ctgactcgat ccatgtatga tttctatgct attcaagctg
     3181 aactcatcaa ggtagaggaa aatgttgcca ctatatttct acctcgctct gaaatggaaa
     3241 tggtctggga aaaacaacta aaagatatta ttgtagtagc tggttttgaa atttatgacg
     3301 ctgaaataac tccccactat attttcacca aacctcaaga tacgactagc tcacaagttg
     3361 aagaagctac aaatttaact ctttatgact atagtccaaa gttagtatct attccttatt
     3421 cagatacggg attaaaagaa aagtatacct ttgataactt tattcaaggg gatggaaatg
     3481 tttgggctgt atcagccgct ttagctgtct ctgaagattt ggctctgacc tataaccctc
     3541 tttttatcta tggaggacca ggccttggta agactcactt attaaacgct attggaaatg
     3601 aaattctaaa aaatattcct aatgcgcgtg ttaaatatat ccctgccgaa agctttatta
     3661 atgactttct tgatcaccta agacttgggg aaatggaaaa gtttaaaaag acctatcgta
     3721 gtcttgatct tttgttaatc gatgatatac agtcactcag cggaaaaaaa gtcgcaactc
     3781 aggaagaatt tttcaatacc tttaacgccc ttcatgacaa gcaaaaacag attgtcctaa
     3841 cgagtgatcg tagtccaaaa catctagaag ggctcgagga gaggcttgtc acgcgtttta
     3901 gttggggatt gacacaaact atcacacccc ctgactttga aacacgtatt gccattttac
     3961 aaagtaagac ggaacattta ggctacaatt tccaaagtga tactctagaa tacctagctg
     4021 ggcaatttga ttcaaatgtt cgagatcttg agggagccat caacgacatc actttaattg
     4081 ccagagtaaa aaaaatcaag gatatcacta ttgatattgc tgcagaagcc attagagccc
     4141 gcaaacaaga tgttagccaa atgctcgtca tcccaattga taaaatccaa actgaagttg
     4201 gtaactttta tggtgttagt atcaaagaaa tgaagggaag tagacgcctt caaaatattg
     4261 ttttggcccg tcaagtagcc atgtatttat ctagagaact aacagataat agtcttccaa
     4321 aaattgggaa ggaatttggg ggaaaagatc ataccacagt cattcatgcc catgccaaaa
     4381 taaaatcttt gattgatcaa gacgataatt tacgtttaga aattgaatca atcaaaaaga
     4441 aaatcaaata atttgtggat aacttttagt tttttatctt ttttatccac attttttaaa
     4501 caagctaaaa aacttgatat gacttgttta aaggctgttt tccacagatt tcacagactc
     4561 tattattact attatctttc taatactaaa aataaataaa ggagaatcca tgattcattt
     4621 ttcaat
//
LOCUS       DAVLFB010000113         4522 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLFB010000113 DAVLFB010000000
VERSION     DAVLFB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.113"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..356)
                     /locus_tag="VXD58_001886"
     CDS             complement(<1..356)
                     /locus_tag="VXD58_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV0676528.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTA"
     gene            478..1224
                     /locus_tag="VXD58_001887"
     CDS             478..1224
                     /locus_tag="VXD58_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV0676529.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1237..2217
                     /locus_tag="VXD58_001888"
     CDS             1237..2217
                     /locus_tag="VXD58_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV0676530.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            2412..2732
                     /locus_tag="VXD58_001889"
     CDS             2412..2732
                     /locus_tag="VXD58_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0676531.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            2778..3086
                     /locus_tag="VXD58_001890"
     CDS             2778..3086
                     /locus_tag="VXD58_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0676532.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3103..4401
                     /locus_tag="VXD58_001891"
     CDS             3103..4401
                     /locus_tag="VXD58_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0676533.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
BASE COUNT         1515 a          580 c          896 g         1531 t
ORIGIN      
        1 atggcagtgt gtatgtgatg ttctttagct gattttaaga tggctttagc aaattcaaac
       61 tgagcaaata tttcacctcc tgataaagtt aaacctccac cggattcttc gtaaaattct
      121 ttgtctttta atacctctgt aataatttct tctacagtct tttcttcacc gactaaggtg
      181 aatttctctc gttgagcatc tttcattttt tcaggtttca ttctttgaga ttcaggatta
      241 gaacaccatg gacagcgcag aggacatcct tttaaaaaaa cagttgtacg aatacccggg
      301 ccgtcatgaa ttgaaaagtg ttgaatatta aaaataattc ctttagatat ttccataaga
      361 gttcctcctc ttcataagtc tatcctatca caaaaacgaa agaaaaacaa ttacgaacga
      421 aagtttaatt tgttgttttt tgctttttat tttggtagaa taaaacggag gtgtaaaatg
      481 aaaagattgg aacaaattat taaattagta tcagaatatg aaaagatcga cgttaataca
      541 ttatcggaaa aattaaatgt atcgaaagta acgattagaa aagatttaga taaattagag
      601 tcaaaaggtt tattacacag agagcatgga tatgctgtat taaatagtgg agatgactta
      661 aatgtacgtt tgtcaattaa ctatgaaatt aagagaaaaa ttgttcagga agcagtaaaa
      721 ttggtgtcag ataatgaaac aataatgata gaatctggat cgacctgtgc tttacttgct
      781 gaggaaattt gcaagcaaaa aagaaatgtt acgattgtaa caaattcatt ttttatagca
      841 aattttgtga gagcttatga ttcatgtcgt attattgttc ttggtggaga gtttcagaag
      901 gattcacagg ttactgtggg acctttatta aaagaaatga tacagacttt tcatgtgcat
      961 caagcttttg ttgggacaga tggttacgat aaagagatgg gctttacagg aaaagattta
     1021 atgcgcagtg aggtagttca atatatttca gcagcgtcgg ataaagtcat tgtactaact
     1081 gactcaagta aatttgataa aaggggtaca gtaagaagat ttgctttaag tcaagtctat
     1141 gaagtaataa cagacgaaaa actttctaaa caaaatatag ctacattaga aaatgctggg
     1201 ataatggtta aggtagtttc gtaagaggtt aaatgtatga atcaagatag gaataaactg
     1261 ctctctaaaa ttgcttatct gtattatatt gaaaacttaa atcagtcaca aatagcagca
     1321 aaattaggaa tttatagaac ctctattagt agaatgttaa cagaagcaag gaatgcagga
     1381 attgttaaaa ttgaaataga gaattttgat accaatatgt ttaagttgga aaattatgta
     1441 aaagaaaaat atggtctgga aagtttagaa attattccaa atgaatttga tgataatcca
     1501 acaattttat ctgaaagaat ttctcaagtt gcagcaggtg tccttaggaa tctaattgat
     1561 gataatatga aaattggctt ttcttggggg aaaagtttaa gtaatttagt agatttaatt
     1621 cacagtaaaa gtgtccgaaa tgttcacttc tatcctctag caggtggtcc tagtcacata
     1681 cacgctaaat accatgtgaa tacactgatt tatgaaatgt ctagaaaatt tcatggagag
     1741 tgtacattta tgaatgcaac gattgtgcaa gaaaataaat tgttagcaga tggtattttg
     1801 caatcaagat attttgaaaa tttgaaaaat agttggaaag atttagatat agctgtagtc
     1861 ggaattggtg attttagcaa taaaggaaag catcaatggt tagacatgct tacagaggat
     1921 gactttaaag aattgaccaa agttaaaact gtaggagaaa tttgttgtcg attttttgat
     1981 tcaaaaggta aagaagtcta tgaaaactta caggaaagaa cgatagcaat ctctttggaa
     2041 gatttaaaaa atattcctca aagtttagct gttgcttacg gtgatacgaa agtatcttcg
     2101 attctttctg tcttgcgtgc taatttagta aatcatttga ttacagacaa aaatacaatt
     2161 ttaaaagttt tggaagaaga tggggatttg acttttagag agattctagg tgagtgaaaa
     2221 tgatagactg atcagtttat cgttttttct ttttagtcga ttgcacattt gtgctcatat
     2281 aaacaaaaat agtttatctg ttgtttttgg attgacaact ttattatgta gttgtattct
     2341 atagttacaa aagaaaattt taaaatttca aatgaaaaaa gctttttaca tagtgaaatg
     2401 aggaggaatt tatggaaatt attgttccag atcaaattat catgggttta attttatatg
     2461 ctggtgatgc gaaacaacat atttataaag cgttagatta cataaaaaat ggtacatgtg
     2521 aacggtgtga agaagaaatc cagttagctg atgccgcctt attagaagcc cataatctac
     2581 aaacaaaatt cttggcacaa gaagcgtctg gtacaaagac agaaattaca gctctctttg
     2641 ttcattcaca agatcatctc atgactagta tgacggagat taatttaatc aaagaaatta
     2701 ttagtttgag aaaagaactt cataaaaaat aatactagag tattatcatt gttattaaca
     2761 tagaggagga aaacataatg gtgaagattg gtttgttttg tgcagcaggt ttttctactg
     2821 gtatgcttgt aaataatatg aaaattgcag cgcaagctag tggagttgag gcagaaatag
     2881 aggcgttttc tcagtctaaa ttagcggatt atgcgccaaa tatagatgtt gcactattgg
     2941 gtccacaagt tgcttataca ttagataaat caaaagaaat ttgtgataag tgtgatgttc
     3001 cgatagctgt tattccgatg atggactatg gtatgttaga tggaaaaaaa gtattagatt
     3061 tggccctatc tttgattagt gggtaagaaa aggagattta ttatggcaaa gatggatgtt
     3121 cagaaaatca ttgcaccgat gatgaagttt gtgaatatgc gtggcattat agctctaaaa
     3181 gatgggatgt tagcaatttt gccattgaca gtagttggta gtttattctt gattatggga
     3241 caattgccgt tcgaaggatt aaataagagc attgctagtg tttttggagc taattggaca
     3301 gagccgttta tgcaagtata ttcaggaact tttgctatta tgggtctaat ttcttgtttt
     3361 tcaattgcct attcttatgc taagaatagc ggagtagagg ctttaccagc tggagttcta
     3421 tctgtatctg cattctttat tttgctaaga tcatcttata tccctaaaca aggtgaggca
     3481 attggggatg ctattagtaa agtttggttt ggaggtcaag gaattatcgg tgctatcatt
     3541 ataggtttgg tagtaggaag tatttatacc ttctttataa agagaaaaat tgttattaag
     3601 atgccagaac aagttccaca agctattgcc aaacagtttg aagcaatgat tccagcattt
     3661 gtaattttct tactttctat gattgtatat attttagcga agtcattgac taatggcgga
     3721 acattcatag aaatgattta ttctgctatt caagttccat tgcaaggttt aactggatct
     3781 tggtatggtg caattggaat tgcattcttt atatcatttt tgtggtggtt tggtgttcat
     3841 ggacaatcgg tagtaaatgg agtagtgaca gctctgcttt tatctaatct tgatgctaat
     3901 aaagctatgt tagcctctgg taatctatca ttagaaaatg gtgcacatat tgttactcaa
     3961 caatttttag attcattttt aattctatca ggttcaggga ttacgtttgg tcttgtagtt
     4021 gccatgcttt ttgcagcaaa atcaaaacaa taccaagcct taggaaaagt tgccgctttt
     4081 ccagcaatat ttaacgtaaa tgagccagtt gtatttggat ttccgattgt catgaatcca
     4141 gttatgtttg tacctttcat tcttgttcct gtacttgcag ctgtgatagt atatggagct
     4201 attgcaacag gtttcatgca gccattctca ggggtaacat tgccttggag tacaccagct
     4261 attttatcag gatttttggt gggtggatgg caaggagtta ttactcagct ggtgatatta
     4321 gcgatgtcta cattggttta ttttccattc tttaaagtac aggatcgttt agcttaccaa
     4381 aatgaaatca aacaatctta gaggtatttg tgtgttactg ttaaactcac acatttgtgc
     4441 taaaaattag agagttaaaa tttttctagt taaaagcttg aaaatttcta taaaaatcgg
     4501 tattatattt tcgaaagaaa ta
//
LOCUS       DAVLFB010000114         4521 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLFB010000114 DAVLFB010000000
VERSION     DAVLFB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.114"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            19..1228
                     /gene="sstT"
                     /locus_tag="VXD58_001892"
                     /pseudo
     CDS             19..1228
                     /gene="sstT"
                     /locus_tag="VXD58_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1296..2249
                     /locus_tag="VXD58_001893"
     CDS             1296..2249
                     /locus_tag="VXD58_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV0676534.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            2392..2772
                     /locus_tag="VXD58_001894"
     CDS             2392..2772
                     /locus_tag="VXD58_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV0676535.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETVAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            2871..3092
                     /locus_tag="VXD58_001895"
     CDS             2871..3092
                     /locus_tag="VXD58_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV0676536.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3109..3423
                     /gene="trxA"
                     /locus_tag="VXD58_001896"
     CDS             3109..3423
                     /gene="trxA"
                     /locus_tag="VXD58_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV0676537.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3728..4261
                     /gene="queF"
                     /locus_tag="VXD58_001897"
     CDS             3728..4261
                     /gene="queF"
                     /locus_tag="VXD58_001897"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV0676538.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            4311..>4521
                     /locus_tag="VXD58_001898"
     CDS             4311..>4521
                     /locus_tag="VXD58_001898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676539.1"
                     /translation="MLKAVFEVVDKTDEVSSKHCFEVADRTDKVSLKHCFEVADRTDE
                     VSLKHCFEVADRTDEVSLKHCFEVVD"
BASE COUNT         1289 a         1008 c          891 g         1333 t
ORIGIN      
        1 cacaaaaagg agattattat gaaaagaatc attcatgctt ggaataaggc aagccttgtc
       61 aagcgtatct tgattggtat gctttgtggg gaatcctagg actgaccctt cctaatctct
      121 cagggattgg gctactcggg gatttattcg ttggaggtct gaaagctgtt gctcctatcc
      181 tagtctttgc cctcgttgcc aatgcccttt cccaacatca aaagggacaa gatagcaata
      241 tgaaaactgt tgttttcttg tatatcctat gatagggact ttcgccgctg ctcttgtagc
      301 tgtactagca agtttcatcg tccctattga aattacccta aatagtgcca atactgaaat
      361 tgcaccacca gatgggattg ggcaggtcct cagcaacctc ttgctcaaac tggttgacag
      421 cccagtcaac gccctgctta ctgctaacta tattggaatc ttatcttggg cagtcatttt
      481 tggaatcgct atgagagaag ctagtaaaaa tagtaaagaa ttgctaaaaa ctatcgctga
      541 cgtgacttct aaaattgtcg aatggatcat caatctggct ccatttggaa tccttggtct
      601 tgtttttaaa gccatttctg acaagggagt cggaagcctt gccaactacg gtattttatt
      661 ggttccatta gtaacgacta tgctttttgt tgcccctgtg gtcaaccctt tgattgcctt
      721 cttctttatg agacgcaatc cttactctct agtttggaac tgcctccgtg tcagcggtgt
      781 gacagccttt ttcactcgta gttctgcgac taacattcct gtcaacatga aactctgcca
      841 tgaccttgga ctcaacccag atacctattc tgtttctatc ccactcggtt ctactatcaa
      901 tatggctgga gtagcgatta ccattaacct tttgaccctt gctgcagtta acactcttgg
      961 aattcctgtt gactttgcca cagcctttgt cctcagtgtg gtagcagcta tctcatcctg
     1021 tgatgcttca ggtattgccg gaggttccct ccttcttatc ccagttgctt gtagcctttt
     1081 cggtatttct aacgatattg ccatacaaat tgttggggtt ggttttgtga ttggtgtcat
     1141 ccaagactca tgtgaaacag cccttaactc ttctacagat gtcctcttta ccgccgttgc
     1201 cgaatacgca gcaacccgta aaaaataact catcaaggca agcctgctca tgtcttgtct
     1261 tttacgcttt tattctaact tattaggaaa ttcttatgtc tattagccaa cgtacgacca
     1321 agctcatctt agctacctgt cttgcctgcc tgcttgctta ttttctcaat ctttcgtcag
     1381 cagtttcggc tggaattatc gctctcttga gcctatctga tacgcgtaga agtactttaa
     1441 aactggctcg caatcgtctt ttttctatgc ttctagctct ggctatcggt gttctagctt
     1501 ttcacttgag cggatttcat atctggagtc tcggcctcta tctggccttc tacgttcctt
     1561 tagcctacaa gatgggctgg gaaattggca tcacaccaag cactgttttg gttagccatc
     1621 tcttggttca agagtcaacc tctccagacc ttctagtcaa tgaattcctt ctctttgcta
     1681 ttggtacagg atttgccttg cttgttaatc tctatatgcc ttcacgagaa gaggaaatcc
     1741 agcactacca cacgctggtg gaagaaaagt taaaagatat cctccagcgc ttcaaatact
     1801 atttatccag aggagacgga cgcaaccgag cacagctggt agcagaatta gacacgcttt
     1861 tgaaagaagc cctcagactg gtctatttgg atcactctga ccacctcttt caccagaccg
     1921 actaccatat ccactacttt gagatgagac agcgacaaag tcgtatcctg agaaacatgg
     1981 cccaacagat taacacttgt caccttgccg ccagtgaaag cctgatctta gcgcaactct
     2041 tttcaaaaat tgcaggtcaa ctgagccaga ccaatcctgc ttctgatttg ctagatgaaa
     2101 ttgaacgtta tctggaagtc ttccggaacc gcagtctgcc caagacaaga gaagaatttg
     2161 aaacccgcgc cacccttctt caactcctac gtgaagccaa aaccttcatc caagtaaaag
     2221 ttgattttta ccaaaaatat agacagtaaa aaataaaacc actaaaaaac agagcttgcc
     2281 agcattcttg aaaagtagta taattattgc atgcgcaatc atcttgtgac gcagagactg
     2341 cccgttaatg gactttcttc tatttatgac tctaaaaaga aaggagacac tatgacctat
     2401 ttggaaaaat ggtttgactt caatcggcgt cagaaagaaa ttgaaagtct cttggaagag
     2461 accgttgccc aacagagcga gcaaagtctg accttgaaag agttctacct gctctactat
     2521 ctggacttgg ctgaagaaaa atctctacgc cagattgacc tgccagataa gcttcatctg
     2581 agcccgagcg cagtttctag gatggtggct cgcttggaag ctaaaaattg cggtctactc
     2641 agtcgcatgt gttgccatca agatagacgc tctagtttta tctgcctgac aaatgatggg
     2701 caaaagatac tggcctcgct acaaaagact gtcgaagaaa gcttggaagc aggtttggat
     2761 ttcttgattt aagatgattt tagaaaaagt tgcgtgcgca atcatttttc ttgacattct
     2821 cttttataag gagtaaaata aagttagcat taaacaaagg agttttaaat atgattgaaa
     2881 ttacctatat agatgccagc aagaacgaaa gaactgtgac tttcgagtct tatgaagact
     2941 ttgaacgttc gcaacaagct tgccttatcg gcgtcgcaga ctactaccct gtccaaaaat
     3001 taacttacaa gggtcataat ttggactacc atgggactta tggagatatc ttcttctatc
     3061 tcatgaaaca agatttaagc caatataact aaaaaaagga gaaatacaat ggcaaaagca
     3121 attacagatg caacattcga acaagaaaca aaagacggtt tggtcttagt agacttctgg
     3181 gcaacttggt gtggtccatg tcgtatgcaa ggtccaatct tggacaaatt gtctgaagaa
     3241 ctttcagaag atgtcttgaa aatcgttaaa atggacgttg atgaaaatcc aaacacagct
     3301 cgtgcttttg gaatcatgtc tattccaact cttctcttca aaaaagacgg ccaagttgtc
     3361 aaacaagttg caggtgttca cacagcagaa caaatcaagg ccatcattgc tgaattgagc
     3421 taatcaaacg agagaccaag tactttcttt ggtctctttt ttcttgccct ttgccatttt
     3481 tcaaaaaata tgctagacta tagtagattg aaactagaat agtacacctc tacttctaaa
     3541 acattgttag aaatcgattt aactgtcctg atcgatttgt cctgttctta tttcatttta
     3601 ctatataagt agaatattac gatgtttggg atatgccatc gctaaaaaaa ctcgatttgg
     3661 tattttttag ctccctaata ggagcttttt gcatgctctg aacatttccc attttggaag
     3721 gagtactatg aaacgtcaat cagccttggt cgtctttagt ggtggtcaag attcaacaac
     3781 ctgccttttc tgggttatgc aacactatga aacagtcgaa gctgtcacct ttgcctacgg
     3841 ccaacgtcat cacctcgaaa ttcaaattgc tagagaaatc gctaaggaac agggcattcg
     3901 tcaccatatc ctcgatatgt ctctgctggg acaaatcact gctcagccag actttgcgac
     3961 gattcatatt tcctacattc ctgacaagct ctgtgtcgag tcaaaatccc tcaaactata
     4021 tctatttagc taccgaaatc acggagattt ccacgaaaac tgtatcaaca ccatcgggaa
     4081 agacttggtc aacttgctag accctcgcta tttagaagtc tggggaaaat tcactccgcg
     4141 cggtggcatt tcaatcgacc cctactacaa ctacggtaag caaggaacta agtatgagga
     4201 cttggcagaa caacgcctct tccaacacga cctttatcca gagaaaattg acaaccgcta
     4261 aagtcatact caatgaaaat caaagagcaa actaggaagt tagccgcagg ttgctcaaag
     4321 cagtgtttga ggttgtagat aaaactgacg aagtcagctc aaaacactgt tttgaggttg
     4381 cagatagaac tgacaaagtc agcttaaaac actgttttga ggttgcagat agaactgacg
     4441 aagtcagctt aaaacactgt tttgaggttg cagatagaac tgacgaagtc agcttaaaac
     4501 actgttttga agttgtagat g
//
LOCUS       DAVLFB010000115         4489 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLFB010000115 DAVLFB010000000
VERSION     DAVLFB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.115"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(59..292)
                     /locus_tag="VXD58_001899"
     CDS             complement(59..292)
                     /locus_tag="VXD58_001899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676540.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(285..401)
                     /locus_tag="VXD58_001900"
                     /pseudo
     CDS             complement(285..401)
                     /locus_tag="VXD58_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            complement(616..966)
                     /locus_tag="VXD58_001901"
     CDS             complement(616..966)
                     /locus_tag="VXD58_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV0676541.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            1222..3855
                     /gene="polA"
                     /locus_tag="VXD58_001902"
     CDS             1222..3855
                     /gene="polA"
                     /locus_tag="VXD58_001902"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV0676542.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3940..4377
                     /locus_tag="VXD58_001903"
     CDS             3940..4377
                     /locus_tag="VXD58_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV0676543.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1224 a          833 c         1079 g         1353 t
ORIGIN      
        1 aaataggctc cataatatcc ataggggatt tacccactat ggatattatg gagccatttt
       61 acaacagtgc ctcaaattca gatactgaca tacctaattg ctggctggca acctcagaag
      121 tcagaagacc ttggcgtact atgtctagga aggcaaaaag tttcctttct gcccttcctt
      181 caactttccc acgctctagt ccttgttcaa ggccttcagc cctagcagtt tccaaggcat
      241 agtcctgtgc taacaagact tgttcttctc gcatatgtag ttgactaaac attttcctgt
      301 cctcctcgga ccagctcttg tagtccagca gttgatttgc ttggctaatg gctcgttgcg
      361 gttgctgggt aaagggcttg ttgccgaaaa actccaacca tggcttgcga accttgtctt
      421 tgctggtttc tctgtatttt ttaattccaa gaatgccatc ttaaccagat ggttttcctg
      481 tccattgttg gtaatcgcca atacctcacc tgttgtgtct tcgcgcatac taaagctatg
      541 aaaagccagg tcatctgaga aataattact atccacgata gcaatggcgt aaacaggagc
      601 gatgtgtttg tagctctagt gagtatcacc ttctcgctga cgaatttttt caagattttg
      661 attaacctga ctgcacaggt aagtccacaa gtgattgatg aaaaaattct gatgatggac
      721 ttgaatctca ataattactt gagtaccgtt atccaactcc gccaagacgt ctatactggt
      781 ataaaaatcc tgcaccgagt aaggcatgga gagtaatacg tgaatatcgc ttccctccaa
      841 aatggtcaca ttttttgctg gcaagtccag catatcgcga ataaattgac aagtgatttc
      901 tggattgctg aatattttct tagcaatcaa atcattagtt gggctgatgc ccggatgtct
      961 gagaatcctc ctattcctcc ttctatagtg agttgaaata agatgtgaat aactctatca
     1021 ggaaagtcaa attaatttat agaaatattt tagcagtcaa ggtgtactat tatagattca
     1081 atatattata ttatagcaca tttttaaatc taggtttact aaattctctg gctctatcta
     1141 tttattcgga aaaagtttga aaaatacttg ctctagctct tcccaatggt attttttgat
     1201 tctttccttt ataatgggtg tatggataag aaaaaattat tattgattga tgggtcttct
     1261 gtagcttttc gggcgttttt tgcgctgtat cagcagttgg accgttttaa gaatgcggct
     1321 ggtttgcata ccaatgcgat ttatggtttt cagttgatgt tgagtcattt gttggagcgg
     1381 gttgagccga gtcatatttt ggtggctttt gatgcgggaa agacgacctt ccggacagag
     1441 atgtatgcgg actataaggg tggtcgggcc aagactcctg atgagtttcg tgagcaattt
     1501 cctttcattc gtgagttgct ggatcatatg gggattcgtc actatgagct ggctcagtat
     1561 gaggcggatg acatcattgg gacgctggat aagctagcag agcaggatgg ttttgatatt
     1621 accattgtca gtggggacaa ggatttgatt cagctgacgg atgagcatac ggtggttgaa
     1681 atttccaaga aaggtgtggc tgagtttgag gcctttacgc cagattacct catggaagaa
     1741 atgggcctca caccagctca gtttatcgat ctcaaggcgc tcatgggtga taagtcggat
     1801 aatatccctg gggtgaccaa agtcggtgaa aagacgggta ttaagctctt gctggagtat
     1861 ggttcgcttg aggggattta tgaaaatatt gatggaatga agacttctaa gatgaaggaa
     1921 aatctcatca atgacaagga acaggccttt ttgtctaaaa cactagcgac cattgatacc
     1981 aaggcaccga ttgcgattgg tttagaggac ttggtctata gtggtccaga tgttgaaaat
     2041 cttgggaaat tttacgatga gatgggattc aaacagctca agcaggcttt aaatgtgtcg
     2101 tcagctgatg tggctaaggg tttggatttt actattgttg accaaatcag tcaagatatg
     2161 ctgagtgaag agtctatctt ccactttgag ctttttggtg agaattacca tacggataat
     2221 ttggttggat ttgcctggtc ttgtgaggat aagctctatg ccacagacaa gcttgagctg
     2281 ttgcaagacc cgattttcaa ggatttctta gaaaaaacat ctctgagagt ttatgacttt
     2341 aagaaggtta aagttctttt gcaacgtttt ggtgtggatt tgcaggcgcc tgcttttgac
     2401 atccgtttgg ctaaatacct cctttcgact gtggaggaca atgaaattgc gaccatcgct
     2461 agtctttatg gtcagactta cttggttgat gatgaaactt tctacggtaa gggtgttaaa
     2521 aaggccattc ctgaacgtga gaaattcttg gaacacttag cttgtaaact tgctgttttg
     2581 gtagaaacag agcctatttt acttgaaaaa ctcagcgaaa atgggcaatt agagcttctt
     2641 tatgatatgg agcaacctct ggcttttgtt cttgccaaga tggaaattgc tgggattgtg
     2701 gtcaagaaag agaccttgct tgagatgcag gctgaaaatg agcttgtcat tgaaaaactg
     2761 actcaagaga tttacgagct ggctggtgag gagtttaatg tcaactcgcc taagcagttg
     2821 ggcgtgcttc tctttgagaa attgggactt cctctagaat acactaagaa aaccaagaca
     2881 ggttattcga cagcagtgga tgtgttggag cgtctcgctc ctattgctcc gattgttaag
     2941 aaaatcctgg attaccgtca aattgctaag attcaatcta cttatgtaat tggcttgcag
     3001 gactggattt tggctgatgg aaagattcat actcgctata tgcaggattt gacccagacc
     3061 gggcgtttgt ctagtgtgga tccaaacttg caaaatattc ctgcccgatt ggaacaaggg
     3121 cgcttgattc ggaaggcttt tgtgccagag tgggaggata gtgtgctact cagctctgac
     3181 tattcacaga ttgaattgcg cgttttggcg catatttcta aggatgagca cttgattaag
     3241 gccttccaag agggggcaga tatccatact tcgacagcca tgcgggtctt tggcattgag
     3301 cgtcctgata atgtgactgc aaatgaccgt cgcaatgcca aggcagttaa ctttggagtg
     3361 gtttatggga tttcagactt tggcttgtct aataatttgg gaattagtcg taaggaagcc
     3421 aaagcctaca ttgataccta ctttgaacgt tttccagata ttaaaaacta catggatgaa
     3481 gtggtgcggg aggcgcgtga taagggctat gtagagaccc tctttaagcg tcgccgtgag
     3541 ttgccagata tcaattcgcg caacttcaat attcgtggtt ttgcggagcg aactgctatc
     3601 aactcaccta tccagggttc ggcagcagat attctcaaga ttgccatgat tcagctggat
     3661 aaagccttgg ttgcaggtgg ttatcagact aagatgctgt tacaagtgca cgatgaaatc
     3721 gtccttgaag tgcctaaatc tgaattggta gagatgaaaa aattggtgaa acaaaccatg
     3781 gaagaagcca ttcaactcag tgttcctctt atcgcagatg agaatgaagg ggcaacctgg
     3841 tacgaggcta aataaaaagg gggctagtcc tccttttttg tagtataatt ctgcaaacct
     3901 tttcaaaatg tgctatactg atgaaaaagg aggatttcta tgagtcaaga atttatcaat
     3961 ccaagtgatg gcgtgattcg tcagtatctc gcaacgagta aaacccttgc tgtggtgggt
     4021 ttgtctgacc gtgaagaaac aaccagcaat cgagtaacaa aggaaatgca ggctcggggc
     4081 tataaaatca tcccagtcaa tcccaaggcg gcaggtggcg aaatcttggg tgaaaaggct
     4141 tatgctagcc tcgctgaaat tccttttcct gtagatattg tcaatgttta tcgtcgcagt
     4201 gaatttctgc ccgatgtggc gcgtgatttt ctcaaagctg atgccaagat tttttgggca
     4261 cagctaggac ttgaaagttt agaagcgaaa gagattttgc gtgatggtgg atgtgatgat
     4321 atcgtgatga atcgctgtat caagagagaa catactcgct tgattgagga agcataagaa
     4381 aaaggtagct gatgggctac cttttgtgtt atactcaatg aaaatcaaag agcacactag
     4441 caaactagga aactagccgc gggctgctca aaacaccgtt ttgaggttg
//
LOCUS       DAVLFB010000116         4475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLFB010000116 DAVLFB010000000
VERSION     DAVLFB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.116"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..293
                     /locus_tag="VXD58_001904"
     CDS             <1..293
                     /locus_tag="VXD58_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV0676544.1"
                     /translation="VWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLD
                     QGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            283..603
                     /locus_tag="VXD58_001905"
     CDS             283..603
                     /locus_tag="VXD58_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV0676545.1"
                     /translation="MEANKYKIGIIGSHDIILPFSMIGFDIFSAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            666..2441
                     /locus_tag="VXD58_001906"
     CDS             666..2441
                     /locus_tag="VXD58_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV0676546.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            2442..3827
                     /locus_tag="VXD58_001907"
     CDS             2442..3827
                     /locus_tag="VXD58_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV0676547.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3850..4461
                     /locus_tag="VXD58_001908"
     CDS             3850..4461
                     /locus_tag="VXD58_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV0676548.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1461 a          652 c          960 g         1402 t
ORIGIN      
        1 ttgtgtggtt caataaaata aatccaagct tagattcaat cttttcaact tacgaattga
       61 agatgcagga cgcaacaatt tcatcttctg agttagaatt tttatgtgat ttactattgt
      121 ataaaacttt agatcaagga aggtataatg tagatgggcc gttagttctt gctagatatt
      181 tattgggatg tgagtttgaa gtaaagaatc ttagaatgat catatcagct cttcaaaata
      241 caattccttt tgaatcaata aaagaaagga tacgcccgca ttatggaagc taataagtat
      301 aaaattggca taattggtag ccatgatatt attttaccct ttagcatgat tgggtttgat
      361 atattttctg cctatcaaga acaagaagct ataaatactc taagaaaatt agctcaatct
      421 gattatggtg tcatttatat cactgaagac attgcttcaa tgatattaga tacaattcgc
      481 cattatgatt ctcaagttgt gcctgctatt attttattac cgactcataa acaaggttta
      541 aatttaggat taaaacgtat agaggataat gtagagaaag cagtaggaca caatatttta
      601 taataatgta caaaattgtc tgtaatatta ttctataatt tttggactta gtaaggagaa
      661 taactttgac tcaagggaag attataaaag tatcgggacc tctagttatt gcatcaggta
      721 tgcaggaggc taatattcaa gatatttgcc gtgtaggtaa gctagggtta atcggtgaaa
      781 ttattgaaat gagaagagat caggcatcta tccaagtcta tgaagaaaca tctggtcttg
      841 gtccgggaga acctgttgtt acaactggag aacctctctc ggttgaatta gggccaggat
      901 tgatttctca aatgtttgat ggcatacaac gcccattaga tcgatttaaa ttggctactc
      961 ataatgattt tctagttcgt ggggtagaag ttccaagttt ggatagagat attaagtggc
     1021 attttgattc cactatagca attggtcaaa aagtgagtac gggtgatatt cttggaactg
     1081 tcaaggaaac cgaggtagtt aatcataaaa ttatggttcc ttatggagta tctggagaag
     1141 tcgtttctat tgcatctggc gattttacaa ttgatgaagt tgtatatgaa ataaaaaaat
     1201 tggacggtag tttctataaa ggaacgctta tgcaaaaatg gcctgtccgc aaggcgcgtc
     1261 ctgtttctaa acgtttaatt ccagaagaac cattaatcac aggtcaacga gttattgatg
     1321 cattctttcc agtaaccaaa gggggagctg cagcagttcc tggaccgttt ggagcaggaa
     1381 agacagttgt acaacaccaa gtagctaaat ttgccaatgt tgatattgtt atttatgtcg
     1441 gttgtggaga acgtggaaat gaaatgacgg atgtactgaa tgagtttcct gagttgattg
     1501 accctaatac cggacaatca attatgcaac ggacagttct gattgctaat acttcaaata
     1561 tgcctgttgc tgctcgtgag gcttcaattt atacaggaat taccatggct gagtattttc
     1621 gtgatatggg ctactctgtc gccattatgg ctgattcaac ttcacgttgg gcagaagcgc
     1681 tacgtgaaat gtcaggacgt ctagaagaaa tgcctggtga tgagggttat cctgcttatc
     1741 tgggaagtcg tatcgctgaa tattatgaaa gagcaggacg ttctcaggtt ctagggcttc
     1801 cagaacgtga aggaacgatt actgctattg gagctgtatc gccacctggt ggagatattt
     1861 cagaaccagt tactcaaaac actttacgga ttgtgaaagt tttttggggg cttgatgctc
     1921 cgttggcaca gcgacgtcat tttcctgcaa ttaactggct tacatcttat tcactatata
     1981 aagacagtgt gggcacttat atagatggta aagagaagac agattggaat agtaaaataa
     2041 ctcgtgcgat gaactactta caacgggaat ctagtttaga ggaaattgtt cgtcttgttg
     2101 gaattgattc tctgtctgat aatgaacgac taacgatgga aattgctaaa caaattcgag
     2161 aagattattt gcaacagaac gcttttgatt cggtagatac attcacttcg tttgcaaaac
     2221 aagaagcaat gctaagtaat attctcactt ttgctgatca ggcaaatcat gctttagagt
     2281 tgggttctta ctttacagag attatggaag gtaccgtggc agttcgagac cgtatggcga
     2341 gaagtaaata tgtttcagaa gatagattag atgaaatcaa aattatatca aatgagatta
     2401 cacatcaaat tcatttgata ttagaaacag gaggtctata aatgagtgtt ataaaagaat
     2461 acagaactgc tagtgaagtt gttgggcctc ttatgattgt tgaacaagta aataatgtgt
     2521 cttacaatga gttagttgaa attcaacttc ataatggaga aattcgtcgt ggacaagttt
     2581 tagagatcca cgaagataaa gcaatggttc agctttttga aggatctagt ggaataaatt
     2641 tagaaaagtc taaaattcgt tttgctggtc atgcattaga attggctgta tctgaggata
     2701 tggttggtcg tatttttaat gggatgggaa aaccaattga tggtggacca gatttaattc
     2761 cagagaaata tttagatatt gatggtcaag ctattaatcc tgtatctaga gattatccag
     2821 atgaatttat tcagacaggg atctcctcta ttgatcattt gaatactctt gtacgtggtc
     2881 aaaaattacc agtattttca ggttcgggct tacctcataa tgaattagct gctcagatag
     2941 caagacaagc gactgtttta aattctgatg aaaattttgc ggttgtattt gcagcaatgg
     3001 gtattacttt tgaagaagct gagtttttta tggaagaact cagaaaaaca ggagcgatcg
     3061 atcgttcggt tttatttatg aacttggcaa atgatcctgc aattgagcgt attgcaactc
     3121 cccgcattgc tttaactgcg gcagagtatc tagcttttga aaaagatatg cacgttctag
     3181 ttatcatgac ggatatgact aactattgtg aagcgttacg tgaagtctcg gcagctcgcc
     3241 gtgaagttcc agggagacga ggctatccgg gatatttata tacaaattta tcaactctat
     3301 acgaaagggc tggtcgctta gttggtaaaa aaggttcggt gacacagatt cctattttaa
     3361 caatgccaga agatgacata acacatccaa ttcctgattt aactggatac attactgaag
     3421 ggcaaattat tttgtcgcat gagttgtata atcaaggtta tcgtccacca atcaatgttt
     3481 taccttctct ctctcgatta aaagataagg gatctggaga aggtaaaact cgtggagatc
     3541 atgctccaac tatgaatcaa ctgtttgcag cctatgccca agggaaaaag gttgaagagt
     3601 tagcagtagt attaggagaa tcggctttat ctgatgtaga taaattgtat gtgaggttta
     3661 caaagcgttt tgaagaagag tacataaacc aaggatttta taaaaatcga aatatagaag
     3721 atacgttgaa tcttgggtgg gaattactat caattcttcc tagaacagag ttaaaacgta
     3781 tcaaagatga tttgcttgat aaatacttac ctttggtaga agtttaatcc ggaaatggag
     3841 tgattatcta tggtacgttt gaatgtaaaa ccaactcgta tggaattgaa taacttaaag
     3901 gaacgtttga caacagctga acgtggacat aagttattaa aggataaaag agatgaattg
     3961 atgaggcgat ttatttcttt gattcgtgag aataatcaac ttcggaaaga agtggaaagt
     4021 tatctaattg ataatctaaa atcctttgca gttgctaaat cattaaagaa ttctcaaatg
     4081 gtggaggaat tattttcaat tccatcgaaa gaaattgaat tatttgttga gaaagaaaat
     4141 atcatgagtg taacagttcc tagaatgcat atgaatatta cttctcaaaa tgagaacagt
     4201 gaatacagct atttatcttc taatagtgaa atggatgatg tatttgctac aatgaatagt
     4261 ttaatttata aattactaag actggcagaa gttgaaaaaa cgtgtcagtt aatggctgat
     4321 gaaatagaaa aaacacgtag acgtgtaaat ggtttagaat actcgattat tccaaacttg
     4381 tcggaaacta ttcattatat agaattgaaa ctagaggagg cagaaagagc caatttagtt
     4441 cgtattatga aagtgaagta gatcctttat ttaga
//
LOCUS       DAVLFB010000117         4460 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLFB010000117 DAVLFB010000000
VERSION     DAVLFB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.117"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            10..1860
                     /locus_tag="VXD58_001909"
     CDS             10..1860
                     /locus_tag="VXD58_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV0676549.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2042..2140)
                     /locus_tag="VXD58_001910"
                     /pseudo
     CDS             complement(2042..2140)
                     /locus_tag="VXD58_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2166..2798)
                     /locus_tag="VXD58_001911"
     CDS             complement(2166..2798)
                     /locus_tag="VXD58_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV0676550.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2820..3692)
                     /gene="sdaAA"
                     /locus_tag="VXD58_001912"
     CDS             complement(2820..3692)
                     /gene="sdaAA"
                     /locus_tag="VXD58_001912"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV0676551.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3701..4372)
                     /gene="sdaAB"
                     /locus_tag="VXD58_001913"
     CDS             complement(3701..4372)
                     /gene="sdaAB"
                     /locus_tag="VXD58_001913"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV0676552.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
BASE COUNT         1220 a          904 c         1000 g         1336 t
ORIGIN      
        1 tagatttcta tgaataaaaa actaacagat tatgtgattg atctggtgga aattttaaat
       61 aaacaacaaa agcaggtttt ctggggaata tttgatattt tcagtatggt ggtttccata
      121 attgtatctt atattttatt ttatgggctg attaatccag cacctgttga ctacattatc
      181 tatacgagtt tggccttctt gttctatcaa ttgatgattg gtttttgggg gttgaacgcg
      241 agcattagtc gttacagcaa gattacggat ttcatgaaaa tcttttttgg tgtgactgct
      301 agcagtgtct tgtcatatag tatctgttat gccttcttgc cactcttctc catccgtttc
      361 atcattctct ttatcttgtt gagtaccttc ttgattttat tgccacggat tacttggcag
      421 ttaatctact ccagacgcaa aaaaggtagt ggtgatggag aacaccgtcg gaccttcttg
      481 attggtgccg gtgatggtgg ggctctcttt atggatagtt accaacatcc aaccagtgaa
      541 ttagaactgg tcggtatttt ggataaggat tctaagaaaa agggtcaaaa acttggtggt
      601 attcctgttt tgggctctta tgacaatctg cctgaattag ccaaacgcca tcaaatcgag
      661 cgtgtcatcg ttgcgattcc gtcgctggat ccgtcagaat atgagcgtat cttgcagatg
      721 tgtaataagc tgggtgtcaa atgttacaag atgcctaagg ttgaaactgt tgttcagggc
      781 cttcaccaag caggtactgg cttccaaaaa attgatatta cggacctttt gggtcgtcag
      841 gaaatccgtc ttgacgaatc gcgtctgggt gcagaactga caggtaagac catcttagtc
      901 acaggagctg gaggttcaat cggttctgaa atctgtcgtc aagttagtcg cttcaatcct
      961 gaacgcattg tcttactcgg tcatggggaa aactcaatct accttgttta tcatgaattg
     1021 attcgtaagt tccaagggat tgattatgta cctgtgattg cggacattca agactatgat
     1081 cgtttgttgc aagtctttga gcagtacaaa cctgctattg tttatcatgc ggcagcccac
     1141 aagcatgttc ctatgatgga gcgcaatcca aaagaagcct tcaaaaacaa tatccgtgga
     1201 acttacaatg ttgctaaggc tgttgatgaa gctaaagtgt ctaagatggt tatgatttcg
     1261 acagataagg cagtcaatcc accaaatgtt atgggagcaa ccaagcgcgt ggcggagttg
     1321 attgtcactg gctttaacca acgtagccaa tcaacctact gtgcagttcg ttttgggaat
     1381 gttcttggta gccgtggtag tgtcattcca gtctttgaac gtcagattgc tgaaggtggg
     1441 cctgtaacgg tgacagactt ccgcatgacc cgttacttta tgaccattcc agaagctagc
     1501 cgtctggtta tccatgctgg tgcttatgcc aaagatgggg aagtctttat ccttgatatg
     1561 ggcaaaccag tcaagattta tgacttggcc aagaagatgg tgcttctaag tggccacact
     1621 gaaagtgaaa ttccaatcgt tgaagttgga atccgcccag gtgaaaaact ctacgaagaa
     1681 ctcttggtat caaccgaact cgttgataat caagttatgg ataagatttt cgttggtaag
     1741 gttaatgtca tgcctttaga atccatcaat caaaagattg gagagttccg cactctcagt
     1801 ggagatgagt tgaagcaagc tattatcgcc tttgctaatc aaacaaccca cattgaataa
     1861 aaaagaaaaa cgcatagtat caagttacac aaccttggta atatgcgttt tattatgtag
     1921 agacttatac tcttcgaaaa tctcttcaaa ccacgtcaac gtcgccttgc cgtatgtata
     1981 tgttactgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     2041 cagttccatc tacaacctta aaacagtgtt ttgagcagcc cgcagctagt ttcctagttt
     2101 gctctttgat tttcattgag tattacttca ttttcttctg aaatagagtt gtcgcccagt
     2161 ctatgctatt gaaaatacgc caaaacttct aagggtttgt gagcgatata atcaggttga
     2221 tagtttagta gatctgcttg ctctccaaat ccccaagtga cggccaattt ctgaatacct
     2281 gtttctcgag ctcccagcat atcaaacttg gtatctccga tgatgatggc ttgttctggt
     2341 gctagttgat gtgtctgtaa ggcttggtga atgacatctg ccttatgggg tgcttcaggg
     2401 ctagaaccat aaatgccatc aaagaaatga tggatttcca agtttttggc catgtcttga
     2461 gcagtagatg tattctttgt cgtggtgatg tagagtggat aactgctcga taactcctca
     2521 agcaagtcta taatctgagg aaagagttga gcttcataga tgccttttgc cttatagtaa
     2581 gaacgatata tctgcatggc ttcagaaatt tggtctttgg acaggcaggt cgcaaaacta
     2641 ctttcgagag gtggtcccat aaaaccacga atagttttgg catcagggct aggcaccccc
     2701 agctctttaa aggtataggt aaaggcattg tgaatcccga tagaactatc aacgagggtt
     2761 ccatccaaat cgaaaaaaat cgctgtgata gaggtcatgg tttctcctat ttgataagct
     2821 tattctccga aaatttcttt ttggaggcga cgaccagtag gggtggtagc gagtccacct
     2881 tcagctgttt cacgaaaggc agttggcatg cttgctccta cttggtacat ggcatcgatc
     2941 acttcatcca cagggatttt agattctata cctgccaagg ccatgtctgc tgcgatgaaa
     3001 gcaaagctag ctcccatggc attacgtttg acacagggaa cttcgaccaa acctgcaaca
     3061 gggtcacaga tgaggcctag catattttta atgacaaagg caatagcttg actggcctga
     3121 taaggtgttc cacctgcagc cagagtcaag gcggcagcac tcatagcaga ggctgaacca
     3181 acttcagctt gacacccacc ctcagcacct gagatggagg cattgtttgc gatgactagt
     3241 ccaaaggcac cagcagcaaa gaggaaatcc aattgttgct cgtggctgag gtctaatttt
     3301 tcaatagcag cagtgagaac ggaagggaga caaccggcac ttcctgcggt aggagtagcg
     3361 cagaccaagc ccattttagc attgtgttca ttgactgcga tagcatttcg ggcagcagag
     3421 agaatcgtgt aatctgataa agtttttccg ttttcgatgt agtgatccaa tttggcagca
     3481 tctccacctg tcaggccact acgagattta ttttcattga ggccaagttg gacagaggct
     3541 ttcataactt ccagattgcg ttccatgaga aggaagactt cttcacgttc gcgaccggtc
     3601 aattcaaact ctgttgtaat catgagttct gcgacatttc cttgaaagtc cagatctgct
     3661 tgctcgacca attctttgat agaataaaac atgcttcctc ctatttaaag aaattgacat
     3721 tgtggagatg agggattttt cgaatttctt cgatagcctc atcacagttg cgactgtcaa
     3781 cttcgataat cataatggct ttttcaccag ctttttcacg agtgacattc atctgggcga
     3841 tattgatacc atagcgggaa agcgcctctg taacaagggc aatcatacct ggaatatctt
     3901 gatgaacgat gatgatagtc ggtgtattca tattgagaga gacggcaaaa ccattgagtt
     3961 cggttacctg aatatttcct ccaccgatag aaataccagt cacgctgatg gtcttgtggg
     4021 catttttaac agtaatttta gtggtgttag ggtgaggggc attgctgtct ttctgaatgg
     4081 tccagacaat cttgatacca cgcttgtggg caatttccag actatttgga atttcaggat
     4141 catctgtatc cattcccaaa atacctgcaa caagggctag gtctgttccg tgaccacgat
     4201 aggtcttggc aaatgagtta aaaagttgga attcaacttc tgtcggagta tcatcaaaaa
     4261 tggaagagac aatcttccca atacgaacag caccagcagt atggctacta gatgggccaa
     4321 tcataactgg tccgatgata tcaaagacag attgaaaacg aagtgatttc atcagtttcc
     4381 ccttataaaa attcttatct ctattatatc aaagaatgag gggcttggct ttaattgtgg
     4441 atgaaaacct ttctaatacc
//
LOCUS       DAVLFB010000118         4360 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLFB010000118 DAVLFB010000000
VERSION     DAVLFB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.118"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            364..573
                     /locus_tag="VXD58_001914"
     CDS             364..573
                     /locus_tag="VXD58_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676553.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            563..1051
                     /locus_tag="VXD58_001915"
     CDS             563..1051
                     /locus_tag="VXD58_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676554.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1167..2627
                     /locus_tag="VXD58_001916"
     CDS             1167..2627
                     /locus_tag="VXD58_001916"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV0676555.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            2624..3448
                     /gene="nadE"
                     /locus_tag="VXD58_001917"
     CDS             2624..3448
                     /gene="nadE"
                     /locus_tag="VXD58_001917"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV0676556.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            3798..4349
                     /locus_tag="VXD58_001918"
     CDS             3798..4349
                     /locus_tag="VXD58_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676557.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1303 a          821 c         1010 g         1226 t
ORIGIN      
        1 tacctaaaat acaataaaaa aatcagtcat cttggtatgc tcctgctttc actattcaac
       61 acgtttttga cttatactag gctcatttcc aaaagcatta tataatagtg atatgaaacc
      121 aactaaacta aacaagaaat ataagcaata aaaactcgtt taaaagatcc tactaaagct
      181 aatactaaat aaaaataaaa gagtaaacta ggaagtttat ttcaaacaac ctaaaatact
      241 gattttcggc tgaagataat actggagtgc aaattaatgg ggttataata aatagctgat
      301 agcttgtgtt ggttttggat tttttaagag tagatgagta ttaaaactat aaggaggacg
      361 aaggtggcta aaaatttaaa attaaaatta gctcgggtag agcgtgattt aacacaaggt
      421 caactggcag aggctgtcgg ggtgacgcgc cagactattg gtttgataga agcggggaaa
      481 tacaatccca gtctctcgct ctgccagtcc atttgcagat gtttagggaa aaccctagac
      541 caactatttt gggaggaaga agatgaaaaa tagattttat tattatcaat tactagacga
      601 aagagaagaa caactgttca ataaagcggg ctctgaaagt ttctatatct gcatcgcttt
      661 gtcgctccta tcttatatca tttcagtatt agcaccaagc ctttttaatt ctaatatgct
      721 gctaatcgtt atcatcatag ggacatttta ctttttcaat cgtgcccgtt atctgggagt
      781 gacctactat agtcgttttc attttacgat tttgggttgt tttttcctaa ccttggctat
      841 tacggctctt ttgatgttgc agaattatca attcaacata gaaatttatc agcacaatcc
      901 tttgaatttt aaatacctgt ctgcttgggt cattacttat atcatttacc ttccgtggat
      961 ctttattggc aatcttggtc ttaagagcta tggcgaatgg gctcagaaaa aatttgaaca
     1021 agatatggat gaattggaga gtggagaata gcttgttact cttttctcaa tccagctaaa
     1081 atgtgatata atagtactaa tttattggaa tacatgaaag ttcttgaaaa ttttcatggg
     1141 tttctagcta aggaagtagg aaaagtatgt atccagatga tagtttgaca ttgcacacgg
     1201 acttgtacca gatcaacatg atgcaggttt actttgacca agggattcac aataagaagg
     1261 cggtctttga ggtgtatttc cgccaacagc cttttaagaa cggctatgcg gtttttgcag
     1321 gtttggaaag aattgtgaac tatcttgaag acttgcgttt ttcagatagt gatatagcct
     1381 atttggagtc gcttggttat catggggcgt tcttggatta ccttcgcaat ttcaagttgg
     1441 agttgaccgt tcgttctgcc caagaagggg atttggtttt tgctaatgaa ccgattgtgc
     1501 aggtggaagg acctctagcc caatgtcagt tggtcgaaac ggctcttttg aacatcgtca
     1561 actaccagac cttggtggcg acgaaggcag ctcgtattcg ttcggttatc gaagatgaac
     1621 ccttgatgga gtttgggaca cgtcgggctc aagaaatgga tgcggccatc tggggaacac
     1681 gcgcagctgt gattggtggc gccaatggaa ctagcaacgt gcgtgcgggt aagctctttg
     1741 acattcctgt tttgggaacc catgcccatg ccttggtaca ggtttatggc aatgactatg
     1801 aagctttcaa ggcttacgct gcgacccaca aaaattgtgt ctttcttgtg gatacctatg
     1861 acacccttcg catcggtgta ccagctgcca ttcaggtggc gcgtgagctg ggtgatcaga
     1921 ttaactttat gggtgtgcgg attgactctg gggatattgc ctacatttct aagaaagtcc
     1981 gtcagcaact ggacgaggct ggatttacag aggctaagat ttatgcttct aatgatttgg
     2041 acgaaaatac tatcctcaat ctcaagatgc aaaaggccaa gattgatgtc tggggcgtgg
     2101 gtaccaagct gattacagcc tatgaccagc cagctcttgg tgcagtttac aagattgttg
     2161 caatcgaaga tgaaactggt cagatgcgca atacgattaa gctgtctaat aatgctgaaa
     2221 aagtttctac gccaggtaag aagcaggtgt ggcgcattac cagtcgtgaa aaaggcaagt
     2281 cagaaggcga ctatatcact tatgatggtg tggatattag cgacatgaca gaaatcaaga
     2341 tgttccatcc gacctataca tacatcaaga agacggttcg taattttgat gccgttcctc
     2401 tcttggtgga tatcttcaaa gaaggaatat tagtttacaa cttgcctagt ttgactgaca
     2461 ttcaggatta tgcccgtaaa gaatttgaca agttgtggga tgagtataag cgtgtgctca
     2521 atccgcagca ctatccagtg gatttggcgc gtgatgtatg gcaagataag atggacttga
     2581 ttgataagat gcgcaaggaa gcccttggtg aaggagaaga agaatgagtt tgcaagaaac
     2641 gattatccaa gagctgggtg tcaaaccagt gattgatgcc caggaagaaa tccgtcgttc
     2701 tattgatttc ttaaaaagat atctgaaaaa acatcccttc ctaaaaacct ttgtactagg
     2761 gatttctggg ggacaagact caaccttggc agggcgtttg gcgcaattag ctatggaaga
     2821 actgcgagct gaaacgggag acgatagcta caaatttatc gctgtccgcc tgccatacgg
     2881 agtgcaagct gatgaagcag atgctcaaaa agctctagcc ttcatccagc cagatgtcag
     2941 cttggttgtg aatatcaagg aatcagctga tgccatgaca gctgcagttg aagcgacagg
     3001 tagtcctgtt tcagacttca acaaggggaa tatcaaggca cgttgccgta tgattgctca
     3061 gtatgccctt gctggttccc atagcggagc ggtcattgga acagaccacg ccgcggaaaa
     3121 tatcacaggt ttctttacca agtttggtga cggcggtgcg gatattctcc ctctttaccg
     3181 cctcaataaa cgccaaggaa aacagctctt gcagaaactt ggcgcagagc cagcccttta
     3241 tgaaaaaatc ccaacggcag acctagaaga agataaacca ggcctagctg acgaagtcgc
     3301 acttggagtc acctacgcag agattgacga ctacctagaa ggcaaaacaa tcagcccaga
     3361 agctcaagcg accattgaaa actggtggca caaaggccaa cacaaacgcc acttacccat
     3421 cactgtattt gatgactttt gggagtaaaa aggtccgggg gaccttttta gcttcttgcc
     3481 ctgaaattaa aaagcaagaa aaacctccac tggaggtttt cagcctctca tcttaaaata
     3541 agaaagtgag agaaggtctg ggggatcttg aaccccgagt ttagaaataa gaaaatgagg
     3601 cagattcggt aactcgaaga gttcgatttc atcgtcttac ccctgcaacg atgactaggt
     3661 ttgaaaaagc ttgctagatc gcatttcaaa ccaggcagcg attgcgtcaa gaaattagaa
     3721 gacaaactcg ttttctagct gttactgagt tgagcctttt tactacgagc atagaaataa
     3781 ggaagtgagg tagcatcatg aaatctattg gtacgcaaat attacagaca gaacgtttga
     3841 ttttaagaag atttgtggag agtgatgcag aagctatgtt tcaaaattgg gcttcatccg
     3901 ctgagaatct gacctatgtt acctgggatc cccatcctga tatcgaaatc actcgaaact
     3961 cgattcgcaa ttgggttgct tcctatacta atctcaacta ttataaatgg gccatttgtc
     4021 taaaagaaaa cccagagcaa gtgataggag atatcagcat tgttaagata gacgaggctg
     4081 atttaagctg tgaaatcggc tatgtgctag gcaaggctta ctggggacat ggtatgatga
     4141 cagaggcctt gaaagctatc ttggactttt gttttactca agcaggtttt caaaaggtca
     4201 gagcacgtta tgccagtctc aacccagctt caggtcgtgt catggaaaag gctggaatgt
     4261 cctatctaca aaccattgtt aatggtgtag agagaaaagg ctatcttgcg gatcttattt
     4321 attatggtat aagtagggaa gaatgttgaa ttctattttc
//
LOCUS       DAVLFB010000119         4311 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLFB010000119 DAVLFB010000000
VERSION     DAVLFB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.119"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(200..616)
                     /locus_tag="VXD58_001919"
     CDS             complement(200..616)
                     /locus_tag="VXD58_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676558.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(628..1323)
                     /locus_tag="VXD58_001920"
     CDS             complement(628..1323)
                     /locus_tag="VXD58_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676559.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1517..4018)
                     /locus_tag="VXD58_001921"
     CDS             complement(1517..4018)
                     /locus_tag="VXD58_001921"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV0676560.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
BASE COUNT         1200 a          996 c          824 g         1291 t
ORIGIN      
        1 ctaaaatttt actattttgc aaaaaatttc tatcaccagc acctcaccac gcaaaaaagc
       61 cacctgattg ggtggcttca ttaggagatt gtgatgaaaa aagtttagga ttttattaaa
      121 taaagttagg aggtctttat ttaatgacta tataatacta gaccatcctt aaattttgct
      181 taagaaaaaa caagttttgt tattttttac gattcatcca agtcatccct atacaattat
      241 aggtggataa gatttcaaag cccatagaac gatagaagcc caaggttctt tctgtctctt
      301 ctgtcaccag ctggacttga taggcatctt tgtaatcctc taaagcctct ttcatcaagg
      361 cactaccaat tccttgacgc tgatagattg gtaaaacgat taaatcctga accaatactg
      421 atgagaatcc atctccaacc aaacgaatca agcccaccac agcatcacca tcaagtgcca
      481 gataaattac taatgaatga gataaggcct gctccagcat ctctggttga tgggtataat
      541 ttgtccaacc gacagcctga tagagatgta aaacatcctc tagcttgaca atttcttgct
      601 ttttaatagt aatcatctca acacctctta aagttctctc aagctcttgt actgctgtcc
      661 atttttatca aaattttcag gacgcaacca tgtttccaaa cgatctttga cttgaggcca
      721 gtccttatca atcatagaca accaatccgt atctctcgta cgtcccttat aaaccactgc
      781 ctgacggaag gttccttcat aaataaagct caaacgctcc gcagctcgtc tggatggcag
      841 atttaaggca tcgcatttcc actcgtagcg acgatagttt agctcctcaa aaacatagcg
      901 agctaggaga tactgagctt cagttcctat ccgtgtaccc cttagctctg gagaaaaagt
      961 gactgctccg acttctatta ctcggttatt ctggtcaatg cgcatgagag aaaaagatcc
     1021 caaagcctta ccagtcgcct tgtctatgat tgcatagtaa aaacggtcct tacgagccaa
     1081 catctgatgt aaaaggctaa ccagctcccc catatctgct actggctcct gaaaaaggta
     1141 ggtccacatc tcccgaggag tatccgggcc ataaacagct agcaaatcct ccgcatgctt
     1201 ttccactgaa agagcctcta tcctagcata acgcccttct aagaaatcaa tagaaggcag
     1261 ttcacctggt gtataacctt ccattgactc cccaatcatt tgaccatatt catttactgg
     1321 catagctttc tccttttttc acttttcaaa ctttatagtt gcccatagta taccatactt
     1381 tcataagaag attcaaaaaa tcctccagat tctactctga aggattccta ccttatttca
     1441 caacgatatt aacgagttta gttggctcaa acagtttggg aaactgtttg aggttggaaa
     1501 tcagcaaata aattggttat tttacaacaa tattaaccaa tttatttggt acactaatca
     1561 ctttcacgat ttccttaccg tcaatttctg ctttgacttt ttcatccgct agagcaattt
     1621 cttgcaattc ttcacgtgat aggtctttag cgaccatgag tttggcacgg actttacctt
     1681 tgatttggac aacgatttcg atttcgtctt caaccagttt actttcgtcc caagttggcc
     1741 aagctacata agagattgac tcacctgttt ctgcgactgt ttgccagagt tcttctgcca
     1801 agtgaggtgc aaatggtgca atcaattgaa taaagccttt ggcatagtca acataaagct
     1861 tatcttcctt gttagcagca ttgacaaaga ccataagttg ggcaatagct gtgttgaatt
     1921 tgagagactc aatttgctca gtaacagctt tgactgtttc gttgtaagcc ttgtcaagag
     1981 caccattgtt ttccgcaagg atttctttac ttgtaatcaa acggtaaact cggtcaagga
     2041 acttacggct tccttccaaa ccttcttctg accaagcaat cgaagcatcg agtggtccca
     2101 taaacatttc ataaacacga agggtatcgg caccgtattg ttccaccaca tcatctgggt
     2161 taacaacgtt cttgagcgat ttagacatct tggctggcgc ttgctccaac tcttcccctg
     2221 tttctatatg aaagaaggaa ccatcacgtt tttcaacctt gtcggttgcc acaagagcac
     2281 cacggtggtc acggtagctt gttcccaaaa tcatcccttg gttaaagagt ttttggaatg
     2341 gttccttagt cggaacaaca ccgaggtcat agaggaattt atgccagaaa cgagcataaa
     2401 gcaagtgaag tacagcatgt tccgcaccac ccacgtagat atctactggc aaccattgtt
     2461 tgaggaggtc ctcatcagcc aatttctcag tattgtgcgg gtcaatatag cggaggtagt
     2521 accagcttga accagcccat tgtggcatgg tgttagtttc acgacgacct ttgacaccat
     2581 cttcacgagt cacttcaagc caatctgtca agttagctag tggactttca ccagtacctg
     2641 aaggacggat atccttggtt acaggcaaga caagcggcaa ttcagtttca ggaacagctg
     2701 ttgaagttcc atcttcccaa tgaatgattg gaattggctc accccagtaa cgttgacggc
     2761 taaagagcca gtcgcggagg cggtaggtaa ccttctcctg accacagcct ttttcttcca
     2821 agcaagccac aatcttggca atagcgtctt ctttgttcaa tccatctagg aagtctgaat
     2881 tgacatgcag gccatcctct gtgtaggcag cttcttcgac atttccacct tcaagtactt
     2941 cgacgattgg aaggtcaaat tgtttggcaa attcccagtc acgttggtcg tgggcaggca
     3001 cagccataac cgcacctgtt ccataactag caagaacata gtccgcaatc cagattggca
     3061 tttccttacc attgacaggg ttgatggcat aggcacctgt ccaaacacca gttttttcct
     3121 tagcaaggtc tgtacgaacc aagtcagact taaggctggc ttggtgttta tagtctgcta
     3181 cagcttctgc ttgctctgaa cttgtgatag cgtctactaa ttcatgttca ggagccaaga
     3241 cagtgaaagt cgcaccgaaa agtgtgtccg gacgagtagt aaagactgta aattccttgt
     3301 ctgttccttt tactttgaaa gttacattgg caccagttga tttaccaatc cagttgcgtt
     3361 gcatatcctt gatagactct gaccaatcta gttcatctaa gtcattgagc aagcgctctg
     3421 cgtaagccgt gattttgagc atccattggc gcattggttt gcggacaact ggatagcctc
     3481 cacgctcaga agttccgtca ggaagcactt cttcattggc aatggcagtt cccaattcct
     3541 caacccagtt tactggcact tcagcttcat aggccaagcc tttttcgtaa agcttggtga
     3601 aaatccattg agtccacttg tagtagtttg gatctgttgt attgacttca cgatcccagt
     3661 cataagaaaa tccaagcgca ttgatttgac gtttgaagtt ggcaatgttt tccgctgtaa
     3721 attctgctgg gtcattacca gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat
     3781 cccaacccat tgggtgaagg acattgtagc cttgcgcacg tttgtaacga ctgaggatat
     3841 cggttgcagt ataaccttct gggtgtccta cgtgcagacc agctccagac ggataaggga
     3901 acatatcaag cgcataaaac ttaggttttg atgcatctgt tcctgtctta aatgtatgat
     3961 gttctgccca gtagccctgc cacttaggct caatttcttt atgattgtaa aaactcatgg
     4021 tctctctcca attttgtgat gttactatta taccattttt gagggaattt gaaatgtgtt
     4081 taaaggaaaa actgtatact gtatctcatt aattttgatg actatgaatt gctctatctc
     4141 ccacttttta tcccattgtt atgcagtaaa tcaaggagta agtcaagacg cttatctcgt
     4201 atagcacaaa atacttattt tagaccgaag ataaaactgg aatgctaatt aatgtattca
     4261 caataaatag ccgacaacct atgtcgcttt ttgatttatg aagaacgtat a
//
LOCUS       DAVLFB010000120         4291 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLFB010000120 DAVLFB010000000
VERSION     DAVLFB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.120"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            44..772
                     /locus_tag="VXD58_001922"
     CDS             44..772
                     /locus_tag="VXD58_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV0676561.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1224..1418
                     /locus_tag="VXD58_001923"
     CDS             1224..1418
                     /locus_tag="VXD58_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0676562.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(1784..3094)
                     /locus_tag="VXD58_001924"
     CDS             complement(1784..3094)
                     /locus_tag="VXD58_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV0676563.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            3331..4203
                     /locus_tag="VXD58_001925"
     CDS             3331..4203
                     /locus_tag="VXD58_001925"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV0676564.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         1351 a          696 c          907 g         1337 t
ORIGIN      
        1 ttgttatact actattagaa tattatagag agaaccagat taaatgagta caaaatattt
       61 atttatttac aatgagattc gtgaaaagat tctttgtaat aaatatacca tgaacgaaca
      121 attacctgat gaaatgacat tagctaaaca gtttgcctgt agtcgaatga cgatcaaaaa
      181 agctttagac ttgttagttt ctgagggctt aatttttaga aaacgtgggc agggaacctt
      241 tgttctctct cgtggcagct caaaaagaaa attaatcgtt ccagaaagag atatccgggg
      301 actgacaaaa atatctgaag atgctcattc tacaattgac tcgaggatta ttcacttcaa
      361 attagaattt gcaaatgaat ttttagcaga aaaactacag gtcgctttgc agagtccagt
      421 ttataatatt taccgcctgc gtattattga cggtaaacct tatgttctgg aacaaactta
      481 tatgagtacc gatgttattc caggtattac tgaagatatt ttacaaaaat cgatttacaa
      541 ttacattgaa ggaaagttag gattgcatat tgccagtgct acaaaaatct tacgagcttc
      601 ttctagttca gaaaatgagc aacattactt gcagctcctt ccaacggaac cggtatttga
      661 agtagaacaa gtggcttatt tggataacgg aactccgttt gagtactcga ttagtcgtca
      721 tcgctatgat ttatttgaat ttaattcttt tgcattacga cattcctcct aggagaaaat
      781 gaagccaatc ttttacagac tctagtttaa gaaaaattta aaacagggca agaaggtccc
      841 atctatgctt aaatggtttc tcttttctaa ataagatggc tttaaaagag tgatcgtttt
      901 atccatcatg ttgaaaaata tcttcgtata gcttatagag taggtactga aattgttcac
      961 ctgatctact tcttatagtt atttagtttt aaatagtgtt tcaaatattc ttacactgac
     1021 gagaagtttt tgagtctttt cttgtaacac atatagtata ctgaggttag aatagtagac
     1081 tgtgacttct aacaaattgc tagaaatgaa tttcaatctc ccaatttatt tgttcaaata
     1141 ttgttttaat atattgaata aattctgaat cataatcatt taaaaaaatt ttattttttt
     1201 atttttcatt acgaataata tagatgaagg ggaaagagta tgaaaacaga actgtttctt
     1261 ttgctattag ttcaaaagga gaaaaaatga aagtagaaaa tatttcgtat agggtggatc
     1321 atcgtatatt gtttgataat atttcttttg atacttcgag ttcaggcgtg acattaatta
     1381 ctggtaaaaa tggtacagga aagtcaactt tactatagta gattgaaact agaatagtac
     1441 acatctactt ctaaaatatt gttagaaatc gatttgacta tcctgatcta tttgtcctgt
     1501 tcttatttca tttcactata tctcaaattg agtatgacga agtgcgctcc catgtcctgg
     1561 gaacgcactt tcttcatatt tttcatattc ttgaatccat cgataaagac tattgggatg
     1621 aatttttaaa gttgaactaa tcatttttac aggatgagat ttacagcaga gagtttgaag
     1681 gctttatcaa aggtttttct tggcataatg acttttcctc gtttccactt aattttgtgt
     1741 ctactttatt ataccaagtc cacccttaag ttagataata aatctaactt aaggaagcta
     1801 gaaggatgag aatccaggtg gtcaagagtc ccaaacttaa gctgatgggg acacccagaa
     1861 taatttgctt tttgaaggca aggccacgtt cctctatatt gggaagtgag agttgaatga
     1921 gagaaccagc tgatgaaaag ggtgagatat tagtagatag agcgccaata acggtggctg
     1981 ttgtgagtaa gtgaatatca atctgaggat tttgagcact gatgatagca atgatgggaa
     2041 agagggctgg agctacaacg gatagggtgg aactaaagag tgacatcact ccggctatca
     2101 cacaaaagaa cagaggtaac cagaaatgag gaatggttgt tgtcatgagg tgccctatca
     2161 gtgtgactaa acctgacttg accgctagag acattagtaa gctcatgccg cagagcatga
     2221 taattgtagc ccagggaacc ttagctaaaa tggcttcttg cttccctaat ttgagcctta
     2281 aggcgaggca gaccatgagt attgagacaa agccaatatc aaatgttttt tgataagtag
     2341 ctatccaggc gatgtttggg aaaatgagat gcaacaaggg aaaaagccaa accaaaacca
     2401 tgctgctgat catgagcaag gtggtttgtc tttgaacctt gctgaggagt ggtggttggt
     2461 caatagtcaa ggatgagttt gttcttccct tactatagtg actgtaacag gataataaaa
     2521 gcaagacgat gagtgggtag ataatgctga cgataaagat atgattgcca agtgaaaaag
     2581 cttgctcttc ccatcccatt tgcttaaaca ggccttgaaa gacaatgcct gagctactgg
     2641 ttatcaaatt agcccctcct gaagctcccc aattgacggc ttgagctcca atcaaagggt
     2701 gtttgtccgc tttttgacag agggtaatcg ctagaggaca gcaaacggcc atagtagtga
     2761 aaaatccagc acctaaagca gacaaaaggg ttgccatcag gtataaaatc atgtagaggg
     2821 cgttagggtg ggtgcgtgtg cggtagagaa tgtgttgagc caaaacatca agagtaccgt
     2881 tagttgttgc aacgttataa aagagagaga cgctaaaaat ggtaaaaaag agtgaggttg
     2941 gccaaaaatg aagaagttct ttggggctta atcccatgag agtggttgcg atgaggtaag
     3001 aaaaagcaat agccagcagg ccaatattga ttttggtgcg gtaaccaatt ccaatggcta
     3061 gagcaatggc gctaatcatt attaaatgaa tcattgaatt gtcctttcag ttagaatatg
     3121 gaaataggtt ggcaattttg gcgctcaaaa gaccacctgt ttcctattgg tacttgacta
     3181 ttatacacaa aaaaaataca attgtcttga caattgtatt gacacttgtt agaattaaaa
     3241 atgaggtatt caggcttgtg atttcctatg gaagttaata gtaattgcct ctagtgctta
     3301 caagtgatat taaaaataga ggacttagtg atgtcaatca tttcaactga tttaacccct
     3361 tttcaaatag atgatacatt gaaagcagcc ttgcgagaag atgttcattc cgaagattac
     3421 agtaccaatg ccatttttga tcatcatggc caagccaagg tgtcgctttt tgccaaggaa
     3481 gctggtgttt tagcgggact aaccgttttt caaagggttt ttaccctatt tgatgccgag
     3541 gtgaccttcc agaatcctca tcaatttaag gatggggatc gtttgactag tggcgatttg
     3601 gttttagaaa tcataggctc ggtgagaagt ctcttaacat gtgaacgcgt tgccttgaat
     3661 tttttacaac atttatcagg gattgcttcg atgacagctg cttatgtaga agccttaggc
     3721 gatgattgca ttaaggtatt tgatactcga aaaactactc ctaatttacg tctttttgag
     3781 aaatatgccg tgagagttgg cggtggctat aatcatcgct ttaatttatc agatgctatc
     3841 atgctaaaag acaatcacat tgcggcagta ggtagtgttc agaaggcaat tgctcaagcg
     3901 cgtgcctatg ccccttttgt gaaaatggtc gaggtggaag tggaaagcct tgctgctgcc
     3961 gaagaagctg cggcggcggg tgctgatatt atcatgttgg ataatatgtc attggaacag
     4021 attgaacagg ccattaccct aattgcagga cgttctcgga ttgaatgttc tggaaatatt
     4081 gatatgacca ctattagccg ttttcgtggt ttagcgattg attacgtctc cagtggtagt
     4141 ttaacccata gtgctaagag tcttgatttt tccatgaagg gtttaaccta ccttgatgtc
     4201 taagttgtaa aataaactaa ctttttaaag gatgtctttc ctctagaacg agttttatgt
     4261 cagatagttt aaacgcctct tcaaatatag t
//
LOCUS       DAVLFB010000121         4290 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLFB010000121 DAVLFB010000000
VERSION     DAVLFB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.121"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(76..1164)
                     /locus_tag="VXD58_001926"
     CDS             complement(76..1164)
                     /locus_tag="VXD58_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV0676565.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1182..1850)
                     /locus_tag="VXD58_001927"
     CDS             complement(1182..1850)
                     /locus_tag="VXD58_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV0676566.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLVLAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(1869..>4290)
                     /locus_tag="VXD58_001928"
     CDS             complement(1869..>4290)
                     /locus_tag="VXD58_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV0676567.1"
                     /translation="ARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVLATRAYE
                     RYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLEFVLNEL
                     DLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETGFFGMEG
                     KMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSIFIVIDA
                     IKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSVWFIQCI
                     LQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITINKVRSQS
                     HTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRYHAGLDA
                     RFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAVPGKWGY
                     RCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDKTLRYLT
                     RMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQVGIANLS
                     DSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDDDYADKL
                     VTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDGRNAGTP
                     LAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLIAL
                     LRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDI
                     IGRTEHTL"
BASE COUNT         1345 a          898 c          660 g         1387 t
ORIGIN      
        1 tatttcaatc ttctatagaa ttcaatacgg ggatcaataa aagactacca tttggaaaac
       61 agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc
      121 aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc
      181 ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag
      241 agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg
      301 tctattttcc aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa
      361 atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa
      421 tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa
      481 tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag
      541 cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat
      601 agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc
      661 aataccagac gctaataaac gcttaggggc ttgtgaaata acttttgtat caaccaaaac
      721 taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat
      781 aacagataaa gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg
      841 cttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc
      901 gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc
      961 agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat gtagataatc
     1021 ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa tagggtaatt
     1081 tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct
     1141 agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt
     1201 tgagtaacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg
     1261 aaagctgatt caaaaacatc tgctcctgct gtaacagcat gcgcacctgc agctaaagca
     1321 ttatttactt gtgctacatt tttaaaggat gcagccaaaa tcttactagg agagttctgt
     1381 ctatcaatag caagaactaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc
     1441 attctattat aatatggagc taggtaatcc gctcctgctt cgatagctaa taatccctga
     1501 ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt
     1561 gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt
     1621 cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga
     1681 atagagggtg tggagccaat caattctctt acatctttga ttcgttcaaa aaaattaata
     1741 gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca
     1801 gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc
     1861 tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa
     1921 agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca
     1981 ggatgtttct gagcgtcaat caacgtctct ctggaaacaa cattgtattg aatatggtac
     2041 ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct
     2101 ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct
     2161 gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt
     2221 gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat
     2281 gttccacgtc cctgccctac gttggctgat atagaagatg ttcctgaata acgaattcct
     2341 ccaataggcc ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg
     2401 tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca
     2461 tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccaat
     2521 gcatgccaaa gctgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca
     2581 gctaatgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact
     2641 gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat
     2701 agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc
     2761 attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc
     2821 atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca
     2881 tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga
     2941 tagccccatt tccctggaac tgccgtttca acacatccaa tggcactgta atcataagca
     3001 tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat
     3061 gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct
     3121 aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat
     3181 tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca
     3241 attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga
     3301 accttattaa ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct
     3361 tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa
     3421 cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg aataaaccaa
     3481 actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata
     3541 tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta
     3601 gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag
     3661 tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc tgcttttgct
     3721 ttacgagccc gctcttcaaa acctcttaag ccaaattgca aaagtttctg atagttaact
     3781 gctaggtgag catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata
     3841 taaacagaca cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc
     3901 caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct
     3961 ccatcacgct tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga
     4021 aaaataggag catctttatt ggaagaagct tgatttcccc caatcataga ttcttcttca
     4081 atatagatag tcatattttc caaaatttct ttcagcatat atgcacgttt taggacatta
     4141 ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata
     4201 taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca
     4261 aaatattctg tcttgattgt tgttcttgct
//
LOCUS       DAVLFB010000122         4256 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLFB010000122 DAVLFB010000000
VERSION     DAVLFB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.122"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(19..732)
                     /gene="rsmG"
                     /locus_tag="VXD58_001929"
     CDS             complement(19..732)
                     /gene="rsmG"
                     /locus_tag="VXD58_001929"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV0676568.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            826..1386
                     /locus_tag="VXD58_001930"
     CDS             826..1386
                     /locus_tag="VXD58_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV0676569.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            1388..2287
                     /gene="htpX"
                     /locus_tag="VXD58_001931"
     CDS             1388..2287
                     /gene="htpX"
                     /locus_tag="VXD58_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV0676570.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(2436..3995)
                     /locus_tag="VXD58_001932"
     CDS             complement(2436..3995)
                     /locus_tag="VXD58_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV0676571.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
BASE COUNT         1186 a          803 c          900 g         1367 t
ORIGIN      
        1 atttgtttac taaaaaattt aaagtgggcg tttatttggc ataccagcct tacgtggata
       61 tttatttggt gtttcttttt tcttttctac cactgtgata tagcgcggat ctctattcgg
      121 tagggcgtag ctgagattgt cttcgacctt actaaaaagg agattgaggg cattcttagc
      181 ttctaacaac tcctcaggcg cattgctagc cttgagtgct aatagtttgc caccaacctt
      241 aaggtaggga atagtcaatt cagataggac ctgcatacgg gcaaccgcac gagctgttac
      301 aaaatcatat tgagcacgga agttcttgtc ttgggcaaaa tcttcggcac gtccgtggta
      361 gaaatgaact ccgttcaaat ccagttcttg agccaagagt tgtaggaagt tgatgcgctt
      421 attgagtgaa tcaataatgg tcacatctaa ctccggatag agaattttca ttggtagact
      481 aggaaatcct gccccagccc cgatatcaag aagtttgata gtttcattgg gaatcaaacc
      541 ttgaagaatg ggtgcaatcg aatcgtaaaa atgtttgaga taaacttctt ccttgtccgt
      601 aatcgccgtc aaattaatct tctcattcca ctcgaccaag agctcaaaat aacgttcaaa
      661 ttgttctttt tgctggtccg aaagtggaag attctgctcg gcaagcaagt tgtaaaatgt
      721 ttctggtttc atagttattt ccttctttag tatcatctta ttttaccaca atcattaaaa
      781 atttgatata ctggtactaa tctaaaagta gaaaggactt atatcatgac ttggattatt
      841 cttggagtta tcgctcttat tgttattttt gtgattgtta gctataacgg tttggttaaa
      901 aatcgtatgc aaaccaagga ggcttggagt cagattgatg ttcagttgaa acgtcgaaat
      961 gacctcttgc caaacttgat tgagactgta aaaggttatg ccaaatatga aggttctacc
     1021 cttgaaaagg tggcagaact acgtaaccaa gtggcggcag cgacttcacc agcagaagct
     1081 atgaaagcca gtgatgccct cactcgtcaa gtttcaggta tttttgcagt tgcagaaagc
     1141 tatccagatt tgaaagctag tgctaacttt gttaaattgc aagaggagtt gacaaacaca
     1201 gaaaataaaa tttcttactc tcgtcaactc tataacagtg ttgtcagcaa ctacaatgta
     1261 aaattagaaa ctttcccgag caatattatc gctggaatgt ttggatttaa agcggcagat
     1321 ttccttcaaa cacctgaaga ggaaaagtcg gttcctaaag ttgattttag cggtttaggt
     1381 gactaagatg ttgtttgatc aaattgcaag caataaacga aaaacctgga ttttgttgct
     1441 ggtatttttc ctactcttag ctcttgttgg ttatgcggtt ggttatctct ttataagatc
     1501 tggacttggt ggtttggtta tcgcactgat tatcggcttt atctacgctt tgtctatgat
     1561 ttttcaatcg acagagattg tcatgtccat gaatggagcg cgtgaggtgg atgagcaaac
     1621 ggcaccagac ctctaccatg tagtggaaga tatggctctg gtcgctcaga ttcctatgcc
     1681 ccgtgttttc atcattgatg atccagcctt aaatgccttt gcgacaggtt ctaatcctca
     1741 aaatgcggct gttgctgcga cttcaggtct actagctatc atgaatcgtg aagaactaga
     1801 agctgttatg ggacatgaag tcagtcatat tcgtaattat gatatccgta tttcgactat
     1861 tgcagttgcc cttgctagtg ctatcaccat gctttctagt atggcaggtc gtatgatgtg
     1921 gtggggtgga gcaggtcgca gacgaagtga tgatgaccga gatggaaatg gtcttgaaat
     1981 cattatgcta gtggtttccc tactagctat tgtactggca cctctcgctg caaccttggt
     2041 tcagctcgct atttctcgtc agagggaatt tctggcagat gcatctagtg tcgagctgac
     2101 tcgcaatccc cagggaatga ttaatgccct agataagttg gacaatagca aacctatgag
     2161 tcgccacgtc gatgatgcta gcagtgccct ttatatcaat gatcccaaga aaggtggggg
     2221 gttccaaaaa ctcttttata cccacccacc tatctcagaa cggattgaac gtttaaaaca
     2281 tatgtaaaat gaaggctgga aaaaagtctt taaaatctga aaaacgcata atatcaggtg
     2341 tgaaaacttg atattatgcg ttttactatg ggaagattta cttctttttc tcctaaaatt
     2401 gtgtttttgc cccacctatc tgctatgttg caaatttata aatcttctaa attaactagc
     2461 ttcaaaccgt gttctgtcat gcgatagatg atcgagcaga cttcttttaa gaaacgacgg
     2521 tcatgcgaaa cagtgatgag accgccagga taagtggcaa agagttttct gatttggggt
     2581 tgagaagtgg gagaaaagtt tcgtgtgggt tcatccagca ggagaaagtt tggtttgcgc
     2641 aggactaaat ccaaaagcag gagttttccc tgttgtccgc cagataagga gcgaatttga
     2701 tgctgcattt ctggataact gaaattgaga ctagctaggt gagattggat tttctgtagt
     2761 tcctcttttt ccccagtttt actgagatag gctattgggg ataaatccaa ttgcagtttt
     2821 ttgtggtaat cttgtggcat aaaaccaagt gaaatctctc ttttatcatt cagaagtctc
     2881 tgtaacttgg ctaacagagt tgatttccca acaccatttg gcccgataat accgattttt
     2941 tcttggccac ggacagttag ttgtagtttt tgaaccaaaa ctcggtcgtc aatggacaaa
     3001 ttttcttttt ccagttggac taagacttta gaagctggta atggttggat gtctgaaaag
     3061 aaaagttgga tttgttcctc ttcaagtggt ttttgagtca tggactgagc tgccttttcg
     3121 tagcgttttt cttgtgagag gacagttttc atctttttag ccaataggcg accggcagta
     3181 ctatctttgg tagctcgaag cgcagtttct acattttgct taactctccg atgtttttcc
     3241 atggttttat cgtaggctct ttggttgtta gcagcttgct gactttgttt ggcaaaatta
     3301 gcctttctct gctcactata gctatcatag tctaaatgct ctactagcgt ttccgcttct
     3361 ttacggtgtt tgaccagtcg caagtgaaca atagtgtctg ccgtttcaga aagaaagtct
     3421 tcatcatggg aaatgaaaat aacggtttgc ctggtctttt gaatctggcc ttttagccaa
     3481 tcaactgtct caaggtctag gtcatttgaa ggttcatcta aaaatagaat ctcaaagggt
     3541 ttggctaact catggataag ctgaattttc aaagcttctc cccctgatag attgccaatc
     3601 tcttggtcac ttgcgaaacg attgctatca aaatgcaatt cctccgccaa acgatagagg
     3661 atactgtagt ctaaatcaat agaatctaaa aagaagtagt cgtgtaaagt tttctttttc
     3721 agctcctcgg gaagtttttg aggaatgtag gccagtgact gatagtcaga ttggatgtct
     3781 cccttgatag tgaaatcaga caaatcttcc cccattaaag ttttaagtaa ggttgattta
     3841 ccatttcctt cttcgccaat aatagctacc ttttccccgt cttgtatggt catgcttagg
     3901 tcagagacaa ggtctcgtaa atctttgttt tgtgtgatgg tcaggtgatt gatttttatc
     3961 atactattgc cctttctaga atgtcgatag tgcataacaa aaagctctac tttacctagt
     4021 agagcccaaa gtctatgtca aaaaaactct attaacatgc cagaaaaaga gcataaaaga
     4081 gcacacaaaa agagacaaaa acaagatcta ctaaataaag gttctttatt tgatagactt
     4141 agttgttcat gtctccctta cctccgagta ttagtatctt tatattatac ctttaaggaa
     4201 attttgtcaa cagaaaactg gaaattctat gctctaaaat ccaaaaacag gtccat
//
LOCUS       DAVLFB010000123         4249 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLFB010000123 DAVLFB010000000
VERSION     DAVLFB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.123"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            168..1559
                     /locus_tag="VXD58_001933"
     CDS             168..1559
                     /locus_tag="VXD58_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEV0676572.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            1815..2234
                     /locus_tag="VXD58_001934"
     CDS             1815..2234
                     /locus_tag="VXD58_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676573.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2239..3258
                     /locus_tag="VXD58_001935"
     CDS             2239..3258
                     /locus_tag="VXD58_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV0676574.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3262..3444
                     /locus_tag="VXD58_001936"
     CDS             3262..3444
                     /locus_tag="VXD58_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV0676575.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3446..>4249
                     /locus_tag="VXD58_001937"
     CDS             3446..>4249
                     /locus_tag="VXD58_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEV0676576.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHAESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQ"
BASE COUNT         1420 a          637 c          897 g         1295 t
ORIGIN      
        1 tatactgcta attatcatga gcttctttgc aggcttcgga gctaaattag agtttacatc
       61 ttataacccg tttttatctg ttaaatttgg tttggtcgta gctctgacag tctttgtagt
      121 tcaattgtta gccatactgc cagccatggt taaaagagga aataaaaatg agaatgttga
      181 atcaaacaat tcgtgtgaag agtaaatacc cttacttgat tcgtccccga aatattaaaa
      241 ttgtaagatt gtttatccaa atttttgtct ttttactctt aatatcttcg tggattgcta
      301 taatcccatt tattgctcgt ctagtctttt ccatgttttt tataagtctt ctttttttcg
      361 tagagaagtt ctttcgttct aattttatag caaaattgag aaacaagctc aatatcagag
      421 aagccttgtt ttacatgtta attagtttaa atttatatga tgagctcgat aatgaagtag
      481 tagatacagc agttttatat tttgacgttg agaataataa agttgttgtc tgtgccccac
      541 tatttgggaa ccgttatctg aaaaccttga aaaatttaga ggaatatctc tgtcccactt
      601 tgggcttgtc cttgctgtct aaaaaagaag aaatcgataa aatagtatac gtgttagggc
      661 agaaagaaga aatagaacaa tatgttttta atagtaacac attaacaaga gaatttttta
      721 aagatattcc tagttcaata atcaagctgt caaatacaca gaaatttagt ctaaagtcca
      781 acactaacct aggtatatat ggaagaacgg ggacaggcaa aacaatagct ctgcaatggt
      841 acttatttaa cgcactggct aaaggttgtg gtattgctga taatacttat ttaggcatag
      901 tagatggtaa ggcagctgac ttgtatcgta ttggtgaact tcttcacgaa gaactgggag
      961 aacaagtggc agtaggctct tcaccacaaa tgcttgccca attatcgaga aaattcattg
     1021 agaatatgga tgcaagattt aaaattatca aacagaatag ttcattgaat gctgacattt
     1081 atgagctaga tgagctggga ctggtaccta attttttgtt cgttgatgaa ctcgcttcta
     1141 ttcgtgatag ttgtggctct acgaaacaag gtagagaaac atggaatgaa attctacaaa
     1201 atttaggctt gattgcacgc aaaggacggc aggcaggttg ccacctagtc ctatctactc
     1261 aagaccctaa tgccgaaaac atccccgttg aattgaggaa tcaaatttca gcagttttat
     1321 accttggtgc cccaggggct gatagattga agatggcttt tagcatgtgc gaattagaaa
     1381 acgttcctac agtttcagat agaaaagggg aggctctctt ttatgctgat gggcttaata
     1441 cagtagagcc agtgctaaca attgtgccat ttgttgattt aaaaactaag aaagagtttt
     1501 taaatgtagt tagaaacata ctgccaacta atgacggtct tcctccccta gcgaaatagg
     1561 gcgattgccg gaggcagaat cgagcggagc gctctctttt cgctagggga gggagagcca
     1621 accgactgct ttgcaggagg tcagaaaaaa catccccagt actaataggg ggtgcacaca
     1681 tacatgaaat gtactctaaa cccttgatac gtgaggggtt acggcttact agttgcacca
     1741 aggggtcgca aactcttggg agagtaggga tgagaggtgt ttccatgtat gatacggaat
     1801 taaaggagaa aaatatggtt tacaatatga cagatacaaa cattaaaatt ggattgcttg
     1861 accttgagat gtcacagttt gatattccta agaaagtagt gattttatca gttagggtga
     1921 acaataaata caaagaagtc aacggcgaaa acgtgaagac agatgaagta agtaaaatta
     1981 cttctgctgt tcttgacgct gacaaagtga aagttctaac tgaaatggga atttctactg
     2041 atgacttaaa agctattaac ctagaaatta tcggaaatct agataagatt gcaaaacttg
     2101 ctcaaaatga tgctttgttg aatgttcctg ttgaattgat tcggcctaaa gttcgactag
     2161 cttggaacat ggctaggaat aattggtctg gtgtaaaaat tgtgtgtgag gatattaaag
     2221 ttctaggaga ataatcctat gaatacagtt agaatagatt attttgctgt gacagttaag
     2281 aatatttcgc cagaaaaagt tctgacagac atccttttga ttcctcttga aaactttacc
     2341 ttgaatgatt ggggcataaa taagtataag caacattatt cttgttcaga aattaaggtg
     2401 tattttaatg ctgataggct ttctatgggt gtatttattg aattaaaagg gcaaggttgt
     2461 cgtcaatatg aagaattttt aaacagtaac gaaaataatt ggattgcttt gataagtcgt
     2521 ttatatcatc ataatgccaa ctttactaga ctagatattg cccacgacat atttgacggt
     2581 agcttagatg ttcaacgaat atatgactat tgcaagaaag gattatgtat ctcgaaagca
     2641 aaacactttg aatatcatga gaaatcagtt ctagattctg gtgagcgtgt aggagaaaca
     2701 gttgcaatag gttcacgagg taatcagcag tggtgcatat ataataaacg catggagcaa
     2761 cttggtaagc aagagcttgt agaagtttca tcttggactc gcgcagagct tagatgttgg
     2821 caggagaagg ctaatataat tgcagagcag ttatatttaa aacgtccctt atcgtcaata
     2881 tattttgagg ctatcaacgg tcattatcgc tttattcgtc caaatgctat agatagtaat
     2941 cgttggagaa gaaaaaaggt tgagtggtgg gtaaactatc tagggacaga aaatcagaca
     3001 gttctgagta ttacaagggc taagacgacg ttacgggagt ctgaagtttg gactgagaaa
     3061 caggttgcaa aaacattagc aaaagtctat attgcaaagt atcaggccta tgatgttcaa
     3121 aaggcggaag attatattca aaacttgctc aaagagggac tttcaaagct aactgataat
     3181 gatgaaaagg atatagaaca atatataaga gaacagaata gttcccaact ttggggaacc
     3241 gatggaagga gtgactgaca tatggcagat actatatcaa ataaggattt gatttccatg
     3301 ggctataggc catcaacagc taatgctatt atccatcagg cgagagaatt acttgtatca
     3361 cgaggctata cattttataa tcgcaaacgt ttgatggttg ttccaaaaag tgttgtgaaa
     3421 gagttgttgg gaatggaatt gtgaaatggc ttatatcgag tataaacagc gtggtaagaa
     3481 aagactttgg tccttctcta tacgagaaag gagcaagagc ctactccata aaagcggatt
     3541 taaaacaaaa cgagaagcta aaatagaagc ggagaaagtt cttcataagt taaatactgg
     3601 gagtgtctta agctctagta tgactttatc ggagctttat aatgaatggc ttgatttaaa
     3661 aattttacct agcaatagaa gtgtagttac taaaaaaaaa tatctcatga gaaagaaggt
     3721 catcgaaagg ttatttggaa ataagcctgt atcacaaatt aagcctagcg agtatcaaaa
     3781 aattatgaat gagtatggag agactgtatc gagaaatttc ttaggaagat tgaattcttg
     3841 tattcgggca agtatacaga tggctattgc tgataaggtg ataatagagg attttactgc
     3901 ttatgttgag ttgttctctt ctaagagtgg acaaaaggtt gaggaaaagt atttgcatgc
     3961 tgagtcagat tatcaaaaag ttttagtata tttgaaaaat aagtttgatt atcaaaaatc
     4021 tattgtaccg tatgtaattt atttcctttt taaaactggc atgcgtttct ccgaattgat
     4081 tgctctaact tgggatgaag ttgacgagct taatgagcaa cttaaaactt atcgtcgtta
     4141 caacaccgct attcataagt ttactccacc taaaaacaat acttcaataa gattagttcc
     4201 tattacttca gatatgcttt ccttgttaaa aacattgaaa atattgcaa
//
LOCUS       DAVLFB010000124         4169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLFB010000124 DAVLFB010000000
VERSION     DAVLFB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.124"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(185..913)
                     /locus_tag="VXD58_001938"
                     /pseudo
     CDS             complement(185..913)
                     /locus_tag="VXD58_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1173..1484)
                     /locus_tag="VXD58_001939"
     CDS             complement(1173..1484)
                     /locus_tag="VXD58_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676577.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1499..2785)
                     /locus_tag="VXD58_001940"
     CDS             complement(1499..2785)
                     /locus_tag="VXD58_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV0676578.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            3409..3591
                     /locus_tag="VXD58_001941"
     CDS             3409..3591
                     /locus_tag="VXD58_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0676579.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            3601..3966
                     /locus_tag="VXD58_001942"
     CDS             3601..3966
                     /locus_tag="VXD58_001942"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV0676580.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
BASE COUNT         1378 a          805 c          710 g         1276 t
ORIGIN      
        1 atatctaaga catcgcttct ttgtttaatt tgataggctt taatacctaa gctccgactt
       61 tatcacttaa aaaataaaaa aggacagcta atctcttgcg catcaaggga tttcgccatt
      121 ctttaatctc gtttaagtgt tgtatatgga gctttgcata attttttaaa actaaacaac
      181 tacattaagc acgaactgtg acgctggttc catcttcctt ataaagattg ataagcccct
      241 ctttaagagc tcgaaccatg tctccagctt gaacaagttg tgggacttta atagtcaata
      301 gttccattgg atttggagcg cggtcgattt tattgccttt ggcatcatgc aaatcttcaa
      361 tataggtttc aaaatgacgg aaacctggac cataaaactc aacttggtcc ccttcgttaa
      421 tgacgttccg ttgacgaatg gttgctgttt gtgtcgcatc atcataagaa accacttcag
      481 cgacaaactt gtactcaggg attttacgac gagcaccaaa caactgctca ttttcagatg
      541 gtgtaccata gtaaaatcct gtagccagtt cacgttgggc aaccttccac atctcgtcca
      601 ccaagtcttg tttgatagct tcaaactttt caggactttc aagataggca tccacagccg
      661 ccttgtagca gttggttact gttgatacat agtgaataga ctccatacgt ccttcgattt
      721 ttagactgtc cacaccattt tcaatcatat ctgagatatg gtcaatcata gacatatcaa
      781 cggctgacat tgaaaattct tctggaatct caccctgcaa actcttacgt tctttcccaa
      841 atggcatatc gtaaaggtcg tacttccaac ggcatgactg agaacatcca ccacggttgg
      901 catcacgcat actctagacg gattgttttg acaattctaa ccttttagac tttgcataat
      961 aactgttaaa cagtcgaaaa ttaccccagt tttaacaagt agtcaaaata ccagataaag
     1021 taaaaagggg ctaggaaaaa agtctttaaa atcttaaaaa cgcatagtat caggtattga
     1081 acaatcttga tattatgcgt tttattgtgg gaagatttac ttcattttct cttggaattg
     1141 agttttctcc tacttcaaaa ctagatgatt tgctatttaa aatagcgttc cttatgattt
     1201 tggcaacctg gattaaagcg agaaaaacaa aagaggcaac ttaagctttc tttatattgc
     1261 ttatatgtta atagttttag acaaacacca cataaaatag gcttatcctg tatcaaagat
     1321 aagggataag gctcaaaagg gtgattttct aatgaatcat gacaccgata acaagcataa
     1381 tactttttac aatcatagca ttgtaaagca ataatatcca gcttgctatg gtaatgaatg
     1441 catcttgttt gcttatccac taagaaaccc tgtgcctcta tcatttcctt ttcctttctt
     1501 aagctcgaac tgtgacgctg gttccatctt ccttataaaa attgatgagt ccttctttta
     1561 atgcacgaac catgtctcct gattgaacgg gttgtgggac tttaatagtc aatagttcca
     1621 ttggatttgg agcgcggtcg attttatttc ctttggcatc atgcaaatct tcaatatagg
     1681 tttcaaaatg acggaaacct ggaccataaa actcaacttg gtccccttcg ttaatgacgt
     1741 ttcgttgacg aatggttgct gtttgtaccg catcatcata agaaaccact tcagcgacaa
     1801 acttgtactc aggaatttta cggcgagcac caaacaactg ctcattttca gatggtatac
     1861 cgtagtagaa acctgttgcc aattcacgtt gggcaacctt ccacatctcg tccaccaagt
     1921 cttgtttgat agcttcaaac ttttcaggac tttcaagata ggcatccaca gccgccttgt
     1981 agcagttggt tactgttgat acgtagtgaa tagacttcat acgtccttcg atttttagac
     2041 tgtccacacc attttcaatc atatctggaa tgtggtcaat catagacatg tcaacggctg
     2101 acattgaaaa ttcttctgga atctcaccct gcaaactctt acgttctttc ccaaatggca
     2161 tatcgtaaag gtcgtatttc caacggcatg actgagaaca tccaccacgg ttggcatcac
     2221 gcatactcat gtggtttgaa agagtacaac gtccagagta tgaaatacac atagctccat
     2281 ggacaaaggc ttcaatttca acatctgtac gtttgcggat ctcagctaat tcttccattg
     2341 aaacctcacg cgctaaaacg acacgagtca agcctagctc tttccagaac tcaagggttt
     2401 catagttagt ggcactggct tgggtagaaa ggtggatttc aaggcctggt gcttcagtcg
     2461 ctgcaatcat aatcaaggct gggtcagata cgataactgc tgcaatcccg atatcacgca
     2521 gtttacggaa ccactcacca gcaccagctt catttccttc gtgcataacc atattagccg
     2581 ctacatagac cttggcacca tacttggccg caaactgcac gccttcttcc atctgttcga
     2641 aagtaaagtt tcccgcacgg ctacgaagac cataggcctg accaccgata aagacagcat
     2701 ctgctccata ctgaacagct acctttagct tctctaaagt ccctgcaggt gataaaacct
     2761 caggacgttt taatgttttt gtcatgattt ctcctatttg caatataaaa gtttgacatt
     2821 tttccttctc attttatagt aaataaagga taaaatcaag cctagacgga ttgttttgac
     2881 aattctaatc ttttagactt tgcataataa ctgttaaaca gtcgaaaatt accccagttt
     2941 taacaagtag tcaaaaagtc taaaaaagta aaaaccagta tcccaagata ctgactcgta
     3001 aaaatacata aattagtcct tagatgacta ctcccactct gttgctccta taatggaaaa
     3061 ggattgatac agtctagaat atcgcttttt tatttattat tctgatataa aactgttaga
     3121 attgattttt tgttttatac ccaaatatga atagagacgt agctaataag ttgaataaca
     3181 tatttggaaa aatgagaaat agcaatatta ctagaacttt tagaaacaat caatatttaa
     3241 aaagaaaaaa gctcaaagaa tttattaaac catgttataa tagatatagc tataatttta
     3301 tttataacaa gaggatagaa atgaccgaat tagaaagaaa aaatcgaaaa attagctaag
     3361 aaatattctg ataacttaaa catcaaagtt caagagagag ttcgtgaaat ggcaaatgat
     3421 aataagagcc attatttgat atacagagtt ttaggtattt catttgaaga aggagaaaat
     3481 atcgatttgt atcaaaataa aggtcgtttt ttatacaaat atgctggttc atttttagaa
     3541 gaagctgcag tactatcctt taacgaaaaa tttggtacag aaaatactta aaaagttaac
     3601 attcctaatt ctgaaaatac aaaacctaag acttttgaaa ttgattgttt agtcggagaa
     3661 aaacacgcat acgaaataaa atggtgggat gcaactacag atggagacca tataactaaa
     3721 gaacacacta gaataaaagt tattcataac aaaggatata taccaattcg gttaatgttc
     3781 tactatccaa atagaactca agctataaaa attcagcaaa ctttagaaac attgtataac
     3841 ggtattggag ggaaatatta ttatggagat tctgcctggg aacatttaag agcagtgacc
     3901 ggtattgatt tacttagtat tctaacagat attgcaaata aaaaaacagg ggtaaaatca
     3961 aaatgacagt attaaaagga gataacttag aaatattaaa aactattgaa tcctcaagta
     4021 ttgatttaat ctatatggac cctcctttct ttacacagaa aacccaaaaa ttatctaata
     4081 acaaaaatat tatgtattca ttcgaagata cgtggacttc gattgaggat tacaaagaat
     4141 ttttgtctgt aagattagaa gaatgcaaa
//
LOCUS       DAVLFB010000125         4157 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLFB010000125 DAVLFB010000000
VERSION     DAVLFB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.125"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..1057
                     /gene="dnaN"
                     /locus_tag="VXD58_001943"
     CDS             <1..1057
                     /gene="dnaN"
                     /locus_tag="VXD58_001943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV0676581.1"
                     /translation="IPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAGLLITS
                     LGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEI
                     SASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQ
                     KKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFYTRLL
                     EGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHV
                     HSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVP
                     ADTDEDFMQLITPVRTN"
     gene            1122..1316
                     /locus_tag="VXD58_001944"
     CDS             1122..1316
                     /locus_tag="VXD58_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV0676582.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1400..2515
                     /gene="ychF"
                     /locus_tag="VXD58_001945"
     CDS             1400..2515
                     /gene="ychF"
                     /locus_tag="VXD58_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV0676583.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2587..3156
                     /locus_tag="VXD58_001946"
     CDS             2587..3156
                     /locus_tag="VXD58_001946"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV0676584.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3157..>4157
                     /locus_tag="VXD58_001947"
     CDS             3157..>4157
                     /locus_tag="VXD58_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV0676585.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSD"
BASE COUNT         1420 a          649 c          835 g         1253 t
ORIGIN      
        1 cattcctatt ttatcaacag taaaaattga cgtgaccaat gaaggtatta ctttaattgg
       61 ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa aaaaatgaag atgctggttt
      121 gttaattact tctttaggtt cgatccttct tgaagcttct ttctttatca atgtagtatc
      181 tagtttacct gatgtgactc ttgattttaa agaaattgaa caaaatcaaa ttgttttaac
      241 cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc gaacaatatc cacgaatcca
      301 agaaatttca gcaagcactc ctttaatact tgaaacaaaa ttactcaaga aaattattaa
      361 tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg attttaacag gtgtccactt
      421 cgtattgagt caacacaaag agttaaaaac agttgcaaca gactctcatc gcctaagcca
      481 gaaaaaattg actcttgaaa aaaatagtga tgattttgat gtcgtaattc ctagccgttc
      541 tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa actgtagaga ttttctttgc
      601 caataaccaa atcctcttta gaagcgaaaa tattagcttc tatactcgtc tcctagaagg
      661 aaactatcct gatacagatc gcttgattcc aacagacttt aacactacta ttacttttaa
      721 tgtggtaaac ttacgccagt caatggagcg tgcccgtctt ttatcaagtg cgactcaaaa
      781 tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc gcccatgttc actctccaga
      841 agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt actggtgaag atttgaccat
      901 tagtttcaac ccaacttact tgattgattc tcttaaagct ttaaatagcg aaaaggtgac
      961 cattagcttt atctcagctg ttcgtccatt tactcttgtg ccagcagata ctgacgaaga
     1021 cttcatgcag ctcattacac cagttcgtac aaattaagtg aaagaggttg agcctggctc
     1081 gcctctttta tgatataatc gaaaaagaaa aggagagtag tatgtatcaa gttggaaatt
     1141 ttgttgagat gaaaaaatca cacgcttgta caatcaagtc gactggtaaa aaggctaatc
     1201 gttgggaaat tacacgtgta ggagcagata tcaaaataaa atgcagtaat tgtgagcatg
     1261 ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa taaaattatt gactgagaac
     1321 ccttagttag agggttagca ctttatccct ttttgtgtta taatattagg gattgaaatg
     1381 aaaacggaga atgagaaata tggctttgac agcaggtatc gttggtttgc caaacgttgg
     1441 taaatcaaca ttatttaatg caattacaaa agcaggagca gaggcagcaa actacccatt
     1501 tgcgactatt gatccaaatg ttggaatggt ggaagttcca gatgaacgcc tacaaaaact
     1561 aactgaaatg ataactccta aaaagacagt tcccacaaca tttgaattta cagatattgc
     1621 agggattgta aaaggagctt caaaaggaga agggctaggg aataaattct tggccaatat
     1681 tcgtgaagta gatgcgattg ttcacgtagt tcgtgctttt gatgatgaaa atgtgatgcg
     1741 cgagcaagga cgtgaagacg cctttgtaga tccacttgca gatattgata caattaatct
     1801 ggaattgatt cttgctgact tagaatcagt gaacaaacga tatgcgcgtg tagaaaagat
     1861 ggcacgtacg caaaaagata aagaatcagt agcagaattc aatgttcttc aaaagattaa
     1921 accagtccta gaagacggga aatcagctcg taccattgaa tttacagatg aggaacaaaa
     1981 ggttgtcaaa ggtcttttcc ttttgacgac taaaccagtt ctttatgtag ctaatgtgga
     2041 cgaggatgtg gtttcagaac ctgactctat cgactatgtc aaacaaattc gtgaatttgc
     2101 agcgacagaa aatgctgaag tagtcgttat ttctgcgcgt gctgaggaag aaatttctga
     2161 attggatgat gaagataaaa aagagtttct tgaagccatt ggtttgacag aatcaggtgt
     2221 agataagttg acgcgtgcag cttaccactt gcttggattg ggaacttact tcacagctgg
     2281 tgaaaaagaa gttcgcgctt ggactttcaa acgtggtatg aaggctcctc aagcagctgg
     2341 tattatccac tcagactttg aaaaaggctt tattcgtgca gtaaccatgt catatgaaga
     2401 tctagtgaaa tacggatctg aaaaggccgt aaaagaagct ggacgcttgc gtgaagaagg
     2461 aaaagaatat atcgttcaag atggcgatat catggaattc cgctttaatg tctaaaaatt
     2521 aataaatggt gtcaattagg ttggaaaaaa aattccaacc cttttggctt ttgaaaggaa
     2581 aaataaatga ccaaattact tgtaggcttg ggaaatccag gggataaata ttttgaaaca
     2641 aaacacaatg ttggttttat gttgattgat caactagcga agaaacagaa tgtcactttt
     2701 acacacgata agatatttca agctgaccta gcatcctttt tcctaaatgg agaaaaaatt
     2761 tatctggtta aaccaacgac ctttatgaat gaaagtggaa aagcagttca tgctttatta
     2821 acttactatg gtttggatat tgacgattta cttatcattt acgatgatct tgacatggaa
     2881 gttgggaaaa ttcgtttaag agcaaaaggc tcagcaggtg gtcataatgg tatcaagtct
     2941 attattcaac atataggaac tcaggtcttt aaccgtgtta agattggaat tggaagacct
     3001 aaaaatggta tgtcagttgt tcatcatgtt ttgagtaagt ttgacaggga tgattatatc
     3061 ggtattttac agtctattga caaagttgac gattctgtaa actactattt acaagagaaa
     3121 aattttgaga aaacaatgca gaggtataac ggataaatgg tgaccttatt agatttattc
     3181 tcagaaaatg atcagattaa aaaatggcat caaaatttaa cagataagaa aagacaacta
     3241 atacttggtt tatcaacatc tactaaggct cttgcaattg caagcagttt agaaaaagaa
     3301 gataggattg tgttattgac gtcaacttat ggagaagcag aaggacttgt tagtgatctt
     3361 atatctatct tgggtgagga actcgtctat ccatttttgg tagatgatgc tcctatggtg
     3421 gagtttttga tgtcttcaca ggaaaaaatt atttcacggg ttgaagcctt gcgttttttg
     3481 actgattcat ctaagaaagg gattttagtt tgtaatatcg tagcaagtcg attgatttta
     3541 ccgtctccca atgcattcaa agatagtatt gtaaaaatct cagttggtga agaatatgat
     3601 caacacgcgt ttatccatca gttaaaggaa aatggctatc gaaaagttac tcaagtacaa
     3661 actcagggcg aatttagtct tcgaggagat attttagata tttttgaaat atcccagtta
     3721 gaaccttgtc gaattgagtt ttttggtgat gaaattgatg gtatcaggtc atttgaagta
     3781 gaaacacaat tatcgaaaga aaataagaca gaactcacta tctttccagc tagtgatatg
     3841 cttttgagag aaaaggatta tcaacgagga cagtcagctt tagaaaaaca aatttcaaaa
     3901 actttatcac ctattttgaa atcataccta gaagaaattc tttcaagttt tcaccaaaaa
     3961 caaagtcatg cagactctcg gaagttttta tctttgtgct atgataagac atggactgtc
     4021 tttgattata ttgaaaaaga taccccaata ttctttgatg attatcaaaa attgatgaat
     4081 cagtatgaag tctttgaaag agacttagcg cagtacttta cagaagaatt acagaatagt
     4141 aaagcatttt ctgatat
//
LOCUS       DAVLFB010000126         4025 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLFB010000126 DAVLFB010000000
VERSION     DAVLFB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.126"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            10..963
                     /locus_tag="VXD58_001948"
     CDS             10..963
                     /locus_tag="VXD58_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV0676586.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLQQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSSLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1040..3028
                     /gene="uvrB"
                     /locus_tag="VXD58_001949"
     CDS             1040..3028
                     /gene="uvrB"
                     /locus_tag="VXD58_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV0676587.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3038..3601
                     /locus_tag="VXD58_001950"
     CDS             3038..3601
                     /locus_tag="VXD58_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676588.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3588..>4025
                     /locus_tag="VXD58_001951"
     CDS             3588..>4025
                     /locus_tag="VXD58_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676589.1"
                     /translation="METPKEIEGKSLVHWQMWREAYDDLLPAEFQETMTLERCRLFSQ
                     KYPENTLIAIGDLKVVGFISYGNFRDETMQAGEIIALYVLKDYYGKGIAQKLMKAALT
                     ALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT         1174 a          712 c          935 g         1204 t
ORIGIN      
        1 gacggggata tgaagaacaa aagaatattt aaagacttcc aagcttcaaa aatgagttta
       61 aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct
      121 tttactttgt atggtattgg cttgttagct ctcatcggac ttgctagaaa ttttggagag
      181 gctggtcaaa atcttgcaag ctacttgcag accttgcaac agagcttgac ggataaaaca
      241 agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc
      301 agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat
      361 ttcctcagca atcttctgaa aggatttagt ctaggcttgg cactttttct tctgaccttg
      421 ttaggtttag tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct
      481 cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg
      541 gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt
      601 cttatatcta gcctgttctt taccctgctt catatgggca attctagcct cacccctcta
      661 tctctagtaa atctcttttt attcggagtt gccatggctc tttatcttct caaaactgat
      721 acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt
      781 gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc
      841 aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg
      901 gtattactac tgctgattgt ctatcttgct aataaattaa agaaagaaaa tgaaaggatg
      961 tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc
     1021 acatggagag aggattctta tgatcaatca cattacagat aatcaattta aactagtatc
     1081 aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga
     1141 ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat
     1201 gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct
     1261 ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt
     1321 cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat
     1381 tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc
     1441 ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg
     1501 ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg
     1561 tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgtaatgata ttgatttcca
     1621 acgcggaaga tttcgtgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga
     1681 acatgccttt cgagtagaat tttttggaga cgaaattgac cgtattcgtg aagttgaggc
     1741 tctgacaggt caggtgttgg gagaagtgga tcatttagcg attttcccag cgacacactt
     1801 tgtgaccaat gacgaccaca tggaagttgc cattgcaaag attcaggccg agttggagga
     1861 gcagttagct gtctttgaaa aggaaggtaa actgcttgaa gcccagcgtt tgaaacagcg
     1921 gacagagtat gatatcgaaa tgttgcgtga gatgggctat accaatgggg ttgaaaatta
     1981 ttctcgccac atggatggac ggagcgaagg agagcctcct tatacgcttc tcgacttctt
     2041 cccagatgat ttcttgatta tgattgacga gagtcatatg accatagggc aaatcaaggg
     2101 catgtacaat ggagaccgtt cgcgtaaaga aatgctggtt aattatggtt tccgtttgcc
     2161 gtctgctttg gacaatcgtc ctctccgtcg ggaggagttt gagagtcacg ttcatcagat
     2221 tgtttacgtt tcagcgacac ctggtgacta tgaaaatgaa cagaccgaga cagtgattga
     2281 gcaaatcatt cgtccaacgg gactcttgga tccagaggtg gaagtccgtc cgactatggg
     2341 acagattgat gacctcttgg gtgaaatcaa tgcccgcgtt gaaaaaaatg agcgtacctt
     2401 tatcacaact ttgaccaaga aaatggcaga ggacttgacc gactacttca aggaaatggg
     2461 tatcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg
     2521 tgacctgcgc ttgggtgtct ttgatgtctt ggtcggaatt aacctgctcc gtgaaggaat
     2581 tgacgttcct gaagtgagcc tcgtagctat tctcgatgct gacaaggaag gtttccttcg
     2641 caacgaacgt ggactcatcc agactattgg acgtgctgct cgtaacagtg aaggccatgt
     2701 catcatgtat gcagacacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg
     2761 ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa
     2821 gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga
     2881 agtcgacatc aatagcctca acaaacaaga gcgtaaagaa ctcgtcaaga aactagaaaa
     2941 acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat
     3001 gatgttggaa gtcaaggcct tggattaggg gaatagtatg atttatttaa gaaagttaaa
     3061 gagagaagat ttgatgtctt tatgggaaat ggcttattca caacttaatc cagtttggaa
     3121 acagtatgat gctccctatt atgatgatta tcagtatttt tcaaatttta aagaattcga
     3181 actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt ttgttgatga
     3241 taaactagtt gggactgttt cgcgttattg ggtatgtaaa caaacaagat ggatggaatt
     3301 gggaattggt atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt
     3361 gcagtggata gataggacgt ttcaggatta cttggagttg gagcatctgg gtttgacaac
     3421 ttggtcaggg aatcttggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc
     3481 tcatattcca aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat
     3541 tttgagagaa gactgggaga aaataaatga cggttattat caaatcaatg gaaactccta
     3601 aagagataga aggtaaatcc cttgttcact ggcaaatgtg gagagaggct tatgatgacc
     3661 ttttgcctgc agaatttcag gagacaatga cattagaaag atgtcgactc tttagtcaaa
     3721 agtatccaga aaatacattg attgcgatag gtgatctgaa ggtagttggt tttatcagtt
     3781 atggtaactt tcgtgatgag actatgcaag ctggtgaaat tattgcttta tatgttttaa
     3841 aagactatta tggaaaaggt atcgcacaaa agttaatgaa agcagctttg actgctctta
     3901 atcatttttc tgaaattttc ttatgggtat tgaaagataa caagcgcgcc attgctttct
     3961 atcaaaaaat gggttttact tttgatggac aagaaaaaat acttgaactt ggaaagccta
     4021 taaag
//
LOCUS       DAVLFB010000127         4016 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLFB010000127 DAVLFB010000000
VERSION     DAVLFB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.127"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..716
                     /locus_tag="VXD58_001952"
                     /pseudo
     CDS             1..716
                     /locus_tag="VXD58_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1548..1907
                     /locus_tag="VXD58_001953"
     CDS             1548..1907
                     /locus_tag="VXD58_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676590.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1917..2246
                     /locus_tag="VXD58_001954"
     CDS             1917..2246
                     /locus_tag="VXD58_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV0676591.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            2269..4016
                     /locus_tag="VXD58_001955"
                     /pseudo
     CDS             2269..4016
                     /locus_tag="VXD58_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         1482 a          585 c          785 g         1164 t
ORIGIN      
        1 gaaattagtg cagcagataa attgcatatt gtatgtatat atgattatga acaagaatca
       61 tgggttaatc aatggttaag tgaaaatatt ataagtgaga aagatggaag ttatcaacat
      121 tcactgacta taatgaagga tttcaataat caaaaaatag ttaactatat tgctcatttc
      181 aatagttatg acattttgaa aaaaggttct cacttatcag gtgcatataa acgaaaaatt
      241 ttttctaaag aaaatacacg attttggagt ttaatattaa ctcgaaagaa ttttcgcaac
      301 aacttgatat tctctataaa gaagttggtg tattaagttt gggacaaaaa gttgtagcca
      361 tgcttgattt tttattagca tatagtgatt attctaaaga cttcagacca ttgattattg
      421 atcagcctga agacaatcta gacaatcgtt atatttacag gcatttagtt cagcagttta
      481 gagatgtgaa agctcaacgt caaattattt tagtaacaca taatgctaca attgtaacaa
      541 attctatgac agatcaagtt gttattatgg agtcagatgg agttaacgga tggattgaat
      601 cacagggata tgttagtgaa aaatatataa aaaatcatat catcaatcaa ttagagggag
      661 gaaaagattc cttcaagcat aaaatgtcta tatatgagac ggctttatca gagtagagtc
      721 agaaaaagta ggttagaaat ttagcctact tttttctttg tccgacaggc atagtgtaca
      781 tctgaggtcc aagtcctctg tggatatttg ctgcagatga aaccaatagc gactcctaag
      841 cctgaatatc gtgaggtagg ggggatagga aggaattagc gaaatcaagg ttctacaaac
      901 agaatcgtga cttgaagcca tatatagcgg atgaggaact ctaaaatcca aataggtgtc
      961 gtaacctata tacgtaaatt acgagagtaa actaggaaag atgtacggct tattccgtga
     1021 gcgtttagga cgtagtacaa cgaatcatgg gagtcagctg aacacatagt attgaagaaa
     1081 tttctgtaat ggaaatggag cgaagaagtg aacaattaaa tgaatacctc tctaattaaa
     1141 tttgtcaatt ctaattcctg gtatgaaaag acagtgacct gaaaatgtaa acgatgggag
     1201 ctgatcataa atataggacg gtacatgcag tggtgttaga gattagtcct tacttgattt
     1261 gtgataactt ccccaaattt cttctgctat acttttctca acttttaaaa atccaactaa
     1321 gaattttacc tgggggtttg ggggcggagc cactaagtta tcttatcgtt agctgtcaaa
     1381 actggtaggt tttgataggc tggcgatatg atttttggga tattgtggac acaatatctg
     1441 agctcgcaaa gaccgaacaa ggagagactg aggtctttag gaatcgtact gacaatgtga
     1501 ggcagcctta cattgtcaga caagtataat gttgaaagaa tggtaagatg ggacaagaaa
     1561 ttaagttaat ccgtaagcaa tttagaatca cgagacaaga agaaaaacag ataaaagaaa
     1621 tgatgaggga acaaaaagtg gatagtttct cagaatttct tcgtcaaaat ttattgaaaa
     1681 agaattatca ggatagaatt tttgaaagtt ggttctccct ttggcagtct caaaagtttg
     1741 aacagattag tcgagatgtg tatgaagttc tggttgtcgc aagagaaaat catcaagtga
     1801 ctcaagagca cgtctcaatc ttattgactt gcgttcaaga attgattgca gaagtgaatc
     1861 aagtacagcc actcagtcgt gagtttcgtg aaaaatatat gggttaggag gatgtaatgg
     1921 tttatcgcta tcgtaccaat ctcaaaaaag tatttctaac tgatccagaa ttacaccaat
     1981 tgaatgaacg gattgctaag agcaattgtc aaaatttttc agtctatgcc agaaaagttt
     2041 tgcttaatcc caatatgtca tttgtcacga ttaacactga tacttatgac cagttagttt
     2101 ttgaattgag acgtattgga aataacatca atcaaattgc gcgtgcgatt aatcaaagcc
     2161 atctgatttc tcaggaccaa ttacaagaat tgagtaaagg agtcggagag ttaattaagg
     2221 aagtggacaa agaatttcaa gtgtaggtga aaagactgaa ggagtttcat ggtagttact
     2281 aaacactttg cgacgcacgg aaaaaaatat cgtaggcgtt tgattaaata tatcctcaat
     2341 ccagataaaa cagataatct aaaattagta tctgattttg gcatgagtaa ttacttagat
     2401 tttcctagcc atacagaaat ggtagaaatg tacaatgtta actttactaa taacgacaag
     2461 ttgtacgagt ccagaaatga ccgacaagaa aaacaccaac aaactattca tgcccatcat
     2521 ctcatccaat cattttctcc tgaagataat ctgacacctg aagaaattaa ccgcattggt
     2581 tatgagacca tgatggaatt gacaggaggt cgttttaagt ttatcgtagc aactcacaca
     2641 gacaaagatc atgttcataa tcacatccta atcaacgcta ttgaccgcaa ttcagataaa
     2701 aagttgatat ggaactatgc cttggaacga aacctccgta tgatttcaga ccgtatttct
     2761 aaaatggctg gtgcaaaaat tattgagaaa cgttattctt atcgaggtta taaaaaatat
     2821 agggaatcta gtcataaatt tgaattgaaa caacgtcttt attttttgat gcagcaatct
     2881 aaatagtttg atgatttttt agagaaagca aagcagttgc atgttcagat tgattttagt
     2941 caaaagcata gtcgattcat gatgacggat cgagcaatga caaaacctat tcgaggacgc
     3001 caactcagca aacgagatct atatgatgaa gaatttttta gaacgcattt tgctaagcaa
     3061 gagattgaaa gtcgtttaga atttttgttg aaccgtgtca attctttaga agagttaata
     3121 acaaaagcaa aagaattgaa tctaaccatt gacttaaaac aaaaaaatgt aacttttatc
     3181 ctgaaagaaa ataatcaaaa gataagtttg ggtcatcaaa aaataagtga taagaaatta
     3241 tatgatgtca aattttttca agattatttt aaaaataagg aagtcattgc ttcagaagga
     3301 ttagagaatt tacaggaaca gtaccatgct ttccaagaag aacgagataa ggataaagta
     3361 tccactgaag agattgagga agcctttaag acatttaaga agcctttaag acatttaagg
     3421 aaaaatgaga taccgttcgt gaatttgaag tggaacttgc agagaaccaa atagagaagc
     3481 tagttgataa gggcgtctat atcaaggtgt ctttcggtgt caaatagagc ggtcttattt
     3541 tcattcccaa ctatcaattg gatattatgg aagaagaaaa tcataaaaaa tataaagtct
     3601 acattcgaga aacgtcttct tactttgtct ataataaaga aaacatggat aataattgtt
     3661 tcatcaaggg taggacctta atcagacaat tgagcaacga tagccaaaaa ctcccctata
     3721 gacgaccaac tttaaaaagt cttcaagaga agatttctga aattaatctt atgattgaac
     3781 tatctaacac gaacaaacaa taccaagaaa tcaaagatga gcttgtatta gaaattgcag
     3841 agattgatat gaagctagaa gaaacccaag aaaaaatcgc caccttaaac aagatggcgg
     3901 aagtccttat caatttgaag agtgaagatg agatcggtag aaaattagct aagtatgatt
     3961 ttgaccaaat gaacatgacg gaatcaataa tgttagatcg actcaataca tatatt
//
LOCUS       DAVLFB010000128         3988 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLFB010000128 DAVLFB010000000
VERSION     DAVLFB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.128"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(19..2565)
                     /locus_tag="VXD58_001956"
     CDS             complement(19..2565)
                     /locus_tag="VXD58_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV0676592.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2688..3128)
                     /locus_tag="VXD58_001957"
     CDS             complement(2688..3128)
                     /locus_tag="VXD58_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV0676593.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3103..3972)
                     /locus_tag="VXD58_001958"
     CDS             complement(3103..3972)
                     /locus_tag="VXD58_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV0676594.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
BASE COUNT         1157 a          879 c          789 g         1163 t
ORIGIN      
        1 tttttatttt aggcttattt atgcatttcc gtattgaaga acaactgctt cgactgcagc
       61 tttttcacgg ctaatcaagt caacacgtgc tgcaatttcc ttgattccca taccgatgtt
      121 acggctaaga gcaaggtcag aaagttgcgg ctcaaagaac tccttgtatt ccgccaagcg
      181 ttgctgagtc ttaaatacat gagcaggaag gataacaaag ctatcaaagc tcatatctcc
      241 tccaagggct gccttaatcc aagcccagtt ttcacgcgcc caagaccaag ctgttttctg
      301 agttgcttga tgagctagga attggtaata ccaagcagac aagtcctgtg gtttgaccac
      361 aaatttgtcc ttccaagaag taatcaagtt ttggatatta tccgcatctg tactgtatgc
      421 aagagctgct gccaactggc gtttaaagac agcatctgtt gcgtgagtat aagtatcaag
      481 ataaagtgct aacaagtctt tagtctcatg atgtttcatc tcattaatca gaacttgtga
      541 acgaatagct gctgggagtt ctgcaagatt ctccttgtgt gttgcgaaga tttggctagc
      601 gacttgacga gcttctgcat catttgagcg aatcatcatc gaaacagcca gctgacgaac
      661 caattcatcc tcatctgatt ctccgtcttt agcttcaaaa ccaagacggt catagttatg
      721 acgagccaat ttagcaacca gtcctttgaa ggctgtttca gcatccgttc cttcatcaat
      781 aaagcgctca agggctgaaa tcacttgaga aacagctgaa accaccagat aagactcttc
      841 cttagcaagt ttatcaagaa ctggaagcaa gtctgcataa gaaatgtgcc ctgcctcagc
      901 caacaaacga cgttcttgaa caatttgcag tttgcttgtg ttatcaagtg tctctagctc
      961 agcaagaaca gcttctaaca agtctccttg atagtcggta atatagtggg cagtattttc
     1021 agtgttgaga cgaagagctc cttcattttc agcaagaaga gctgcgtagc cagggatttc
     1081 gatactttca gtttcgagtg tatcaggcaa gcctttccag ttgctattga ggggcaccac
     1141 ccagagacgg ttcttgtctt cgttctcacc gatgaagaat tgtttttgtg aaatcttcaa
     1201 gacatcattt tcaactttga cagtaagaac tgggtaacca ggctgttcca accaagaatc
     1261 catgaaggct gcgacatcac gtcctgacgc ttgaccaagg gcatcccaaa ggtcactacc
     1321 aatggtgttg ctgtattggt gtttttcaaa gtaggcatgc aaacctttag caaaatcagc
     1381 atctcctagc caacggcgaa gcatgtgcat gagacggctt cctttggcat agacgatagc
     1441 gccgtcaaag agtgtattga tttcatctgg atgtttaact tcgacgtgga cagactgaac
     1501 gccatcagta gcgtcacgtt caagagcaag aggtactcca cctgtttgga aatcttcaaa
     1561 gatattccag cttggttcga tggtatccac acagacgtat tccatcatat tagcgaaact
     1621 ttcattgagc caaaggtcat cccaccattt catagtcacg aggttcccaa accattggtg
     1681 agccaattca tgggccacaa caagggcaac ttgttgacgg ctagcaaatg tagagttctc
     1741 atcgacaacc aagtaaactt cacggtaggt cacaagaccc cagttttcca tagcaccagc
     1801 tgagaagtca ggaagggcga tgtggagaga ttgaggaatt ggatacttaa ctccatagta
     1861 atcttcgtaa aactcgatag agcgaacagc gatatccagt gagaaatcaa gatttgaaag
     1921 tggatgtgct ttggttgagt agacacctac cagggtacca tttttagttt tagcggtcac
     1981 cccttgcaaa tcaccagcaa caaaggccaa caagtaagaa gacatgcgag gtgttgtctc
     2041 aaacttccag atacctgttt ccttacggtt ttcaacatcg atttctggca tgtttgacaa
     2101 ggccaattca ccttctgctt ggtcaaagcg aagagagagg tcaaaagttg ctttggcttc
     2161 aggctcatcc acacatggga aagcttcgcg cgcaaaatgg ctctcgaact gagtagacaa
     2221 gacctccttc ttgactccat caactgtata ataagaaggg taaatccctg tcatgttgtc
     2281 tgtaatttta ccagaaaagg caagaaccaa ttcaacttga ccagcctcag ccaattcgat
     2341 atgaagggct tcattgtcat ggtcaactgt aaatggacga gcttgacctg caacttctac
     2401 agaggtgatt tccaagtctt tttggtggag ggagatgcgg tcactctgtg cttgaccagt
     2461 gatggtcact ttcccagaaa aagtcttggt ctcacgactc aaatctaaaa ataaatcata
     2521 atgttcagga acaaattgct taataaaatg ttcaactgct tgcatagttt tctcctattc
     2581 taagtttaag agctgctgct cttcttcaaa caaacttatt atatcatatt ttgcttgaaa
     2641 aaaaaggatt ggacagcgat ttccaaaact ttcttccttc taatagtcta cagtgggtag
     2701 accttgcgaa attcttctaa aacaaccttg ctttcaggtg taaaagtcag ccctacttca
     2761 cccaaacagt ctgtgaaaaa gtcctcatga acctggcgcc aataggcaag tgatttgtct
     2821 ccctcacctt ccttaaaggc atgttgcgca gaaacttgat tgaagagttc aacagaaacc
     2881 tttgtaattt cgacaatgca gacagactga ttttgactat ctaaaatgat atcgaaggtc
     2941 cctacttggg gaagtggttc gtcttctagt acatagaggt cataggctga tgctgttgct
     3001 gtcttttctc ctttaaacac caaatccgct aaaagatctg gttcaactcc aaaagcccaa
     3061 gcatcgattt catctccgat caaaggattg atttgcttgt atttattcca catttcttgc
     3121 ggtatcatgg gtactccttt gtaatttttt actttcttct tttatgtgtt taagatgatc
     3181 tggatggtca atctctaaat caaaaatctc tggaatagaa ctgtagtgga taatgcactt
     3241 gatacccaac tgattcattt tttgtatgaa agaagtattc agatagcctg ctacagcaaa
     3301 atcaatcttg ttctttcttg ctttatcctg catatctctt agcatatcta acattattgg
     3361 actttccata tcatgccatt gactgtttct catagtcgca aaaacaaagg aagtcaaatc
     3421 attcattcca actacaatct ttgaaatgcc cgtttccagt atactagata agtcaaaata
     3481 cgctgacggt aattcaatca tcgttccgac tttcccagta aaaccctgct gacgcaatac
     3541 tgtaatagct tgttttaatt ggtcggcatc attgacaaaa ggaaagataa gagatagatt
     3601 gggattggtt tgataaactt ctgtaacgac atgtgcttca gcctgaaatt catccaaaca
     3661 cgccagtaaa cgcctggttc ctctatagcc aaacaaggga tgcccttcgt caaaaaactc
     3721 tctagtcccc actaaacaat tggcttctgt attcgttaat tcagaaaaac gataccaaac
     3781 ttcttcatct gagtacaggg agcagatagt ctctagataa tcttttacaa attgctgaca
     3841 acttgctaat atgatgtttt gattaagctc tctcaacaaa tattccccac gaatcatgcc
     3901 gacgtggtga aatagttgag gataaatttt ttcaagaatt ttttcgccac taagggcaag
     3961 ttgatttctc atagccattc acctcgtc
//
LOCUS       DAVLFB010000129         3891 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLFB010000129 DAVLFB010000000
VERSION     DAVLFB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.129"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            146..280
                     /gene="rpmH"
                     /locus_tag="VXD58_001959"
     CDS             146..280
                     /gene="rpmH"
                     /locus_tag="VXD58_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV0676595.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            564..1457
                     /locus_tag="VXD58_001960"
     CDS             564..1457
                     /locus_tag="VXD58_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV0676596.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1762..2535
                     /locus_tag="VXD58_001961"
     CDS             1762..2535
                     /locus_tag="VXD58_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV0676597.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELLLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2535..3095
                     /gene="rnmV"
                     /locus_tag="VXD58_001962"
     CDS             2535..3095
                     /gene="rnmV"
                     /locus_tag="VXD58_001962"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV0676598.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3167..>3891
                     /locus_tag="VXD58_001963"
     CDS             3167..>3891
                     /locus_tag="VXD58_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0676599.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     EDLFYEQLPNEEKVWFEATRATIDVIRSGRPEYGETVLDDYFKTIYDKELFLINELEV
                     INLYFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIASEYLFALSNTLFSGLACL
                     DNLSSYDSLGAYIFSLNHIMEKT"
BASE COUNT         1255 a          669 c          924 g         1043 t
ORIGIN      
        1 gggcttgcat tcgaaaaagc tggaaacatt tgcctagagc tattgacaaa agtgaaagaa
       61 tcagatagaa tagtaaagta tgtttagtaa ctgatcactt tatagccggc taaacgaata
      121 caaaatctat gaggaggtat tcatcgtgaa acgtacttat caaccaagta aacttcgtcg
      181 tgcgcgtaaa cacggattcc gtaaccgtat gtcaactaaa aatggtcgtc gcgtattggc
      241 agctcgtcgt cgtaaaggac gcaaagtttt ggctgcataa tccaaacgaa ttctatcaaa
      301 aatcagtagg aactcgagtc tactgatttt tatttttgta aaaaagttca gtagatgcaa
      361 atggattcgg aagcgatgtt atagtagatt gaaactagaa tagtacacct ctgtttctaa
      421 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttatt attttatttt
      481 actataaagt tgaagtaggt ggagatggta cagcaacaat cgtctttaaa gatggttcag
      541 ctattacaat tccaggaaat caattggtag cacaagatcc aaaagcacaa gatagcacta
      601 aaccgactgc tgaaaaatca actgttaaag cacctgctca aagagtagat gtaaaagata
      661 taactcattt aacagatgaa gaaaaagtta aggttgctat tttacaagca aatggttcag
      721 cattagacgg agcgacaatc aatgtagctg gagatggtac agcaacaatc acattcccag
      781 atggttcagt agtgacgatt ctaggaaaag atacagttca acaatctgcg aaaggtgaat
      841 ctgtaactca agaagctaca ctagagtata agctagaaac tataccaggt ggagataagg
      901 gaggcaatac tggaagctca gatgctaatg cgaatgaagg cggtggtagc caggcgggtg
      961 gatcagctca cacaggttca caaaactcag ctcaatcaca agcttctaag caattagcta
     1021 ctgaaaaaga atcagctaaa aatgccattg aaaaagcagc caaggataag caggatgaaa
     1081 tcaaaggcgc accgctttct gataaagaaa aagcagaact tttagcaaga gtggaagcag
     1141 aaaaacaagc agctctcaaa gagattgaaa atgcgaaaac tatggaagat gtgaaggaag
     1201 cagaaacgat tggagtgcaa gccattgcca tggttacagt tcctaagaga ccagtggctc
     1261 ctaatgctgc tcctaagaca acaagtgcac cgcaagcaac tgcaggaaca atgcaagatg
     1321 ttacctacca gtcacctgct ggcaaacaat tacctaacac aggttcagca tcaagtgcag
     1381 cacttgctag tcttggtcta gtggtggcaa caagtggttt tgctttgcta ggaagaaaga
     1441 ctagacgtag aaaatagaac agctagaaaa ttctattctc tacttaaagt tagattataa
     1501 gggggatttt gagaagtcat caatcctagt gatgggtgag aaaagtgaga acccaagata
     1561 atcacatact ttagctgaat aggaatattc tatcaatgta gccaatctct tctgtctcta
     1621 actgtggaat aggagatggg caatatcgga tagaaaagat agcagaatag ctctctattg
     1681 aagagaggag gggaaaccga aaaattaggt gcccctcctc ttttttggta taatagaaga
     1741 tagaaaacga ggttagaaga gatgattttt gatacacata cacacttgaa tgtagaagaa
     1801 tttgcaggtc gtgaggcaga agaaattgcc ttggctgctg agatgggtgt gatacagatg
     1861 aatattgttg gttttgataa accgacgatt gagcgtgcct tggagttggt agatgagtat
     1921 gagcagctct atgcgactat tggttggcat cctacagaag ctggtactta tacagaggaa
     1981 gttgaggctt acttgttgga taagttaaaa cattccaagg ttgtggcttt aggtgaaatt
     2041 ggcttagact accattggat gacagcgccc aaagaggtgc aggagcaggt ttttcgccgt
     2101 cagattcagc tatctaagga cttggatttg ccttttgttg tccatacccg tgatgcgctg
     2161 gaagatacct atgagattat caagagtgag ggcgttggtc ctcgtggtgg tatcatgcat
     2221 tcattttcag ggacgcttga gtgggcagag aagtttgtgg atcttggtat gaccatttcc
     2281 ttctcaggag tggtgacttt taagaaggca actgacctcc aagaagcagc taaagagtta
     2341 cttttggaca agattttggt ggaaacagat gcgccttact tagcacctgt acccaagcgt
     2401 ggtcgtgaaa ataaaacagc ctatactcgc tatgtggtcg actttatcgc tgacttgcgt
     2461 ggtatgacga cagaagagct ggcggtagca acgactgcaa atgcagaacg aatttttgga
     2521 ctggacagca agtaatgaaa gagaaaattt ctcaagttat cgtggttgaa gggcgtgatg
     2581 atacggtcaa tctcaagcgt tatttcgatg tggagaccta tgagactcga ggttctgcca
     2641 tcaatgatca ggatatagag cggattcagc gcctgcacca acgtcatgga gtcattgtct
     2701 ttacagaccc agattttaat ggagaacgga ttcggcgcat gatcatgacg gtcattccaa
     2761 cagttcagca tgcctttctc aagcgagatg aagctgttcc caagtccaag accaaggggc
     2821 gttctctggg aattgagcat gccagctatg aagacctgaa aacggctcta gctcaggtga
     2881 cagaacaatt tgaacatgag agccagtttg acattagtcg tagcgatttg attcgccttg
     2941 gttttctagc aggggcagac agccgtaagc gtagagaata cctcggagag actctccgaa
     3001 tcggctattc caacggcaag caactcctca aacgcctaga gttgtttggg gttactttgg
     3061 cagaagtgga agaagctatg aaatcttatg agtaggaaag atgtagccgt tacaattttt
     3121 taagtttcac agtatttttc gaagcaggta gaataggagg cgtctgatgt taattggtca
     3181 aaaaattaaa gagattcgga tagaaaaagg aattagtcgt ccagattttt gtggagatga
     3241 gcaagaactg acagttcgtc aactgtcgcg aattgaaagt ggagcttcgc aaccgagttt
     3301 gcccaagtta gcctatattg ctcgccggct aggagttcca gtttatagcc ttatgccgga
     3361 tttttcagct cttccttctg cttatttaga attgaaatac cagattttac gtgaaccaat
     3421 ctatggtaaa gaagaggagt acgataagaa ggaagcgtgt ttggaagaga tagaggattt
     3481 attttatgag caacttccta atgaggaaaa agtatggttt gaagctacta gagcaacgat
     3541 agatgttatt cgaagtggac gaccagaata cggggaaacc gtactggacg attattttaa
     3601 aacaatttat gataaggaat tgtttttgat aaatgaatta gaagttatca acttatattt
     3661 tgctatagtg cttacaaaga taaaacaagg tcaaaatcag attgaagaaa ttaacagaat
     3721 tcattcgttt ttagttcgtt tgacaaatca tgtagaatta attgcatcag aatatctgtt
     3781 tgctttaagt aacactttgt tttcgggtct agcctgtttg gataacttgt cgtcttatga
     3841 ttcactagga gcttatatct ttagccttaa tcatataatg gaaaaaacac a
//
LOCUS       DAVLFB010000130         3830 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLFB010000130 DAVLFB010000000
VERSION     DAVLFB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.130"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            219..887
                     /locus_tag="VXD58_001964"
     CDS             219..887
                     /locus_tag="VXD58_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0676600.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            847..1524
                     /locus_tag="VXD58_001965"
     CDS             847..1524
                     /locus_tag="VXD58_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV0676601.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1525..2283
                     /locus_tag="VXD58_001966"
     CDS             1525..2283
                     /locus_tag="VXD58_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV0676602.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2295..3156
                     /locus_tag="VXD58_001967"
                     /pseudo
     CDS             2295..3156
                     /locus_tag="VXD58_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3236..3790)
                     /locus_tag="VXD58_001968"
     CDS             complement(3236..3790)
                     /locus_tag="VXD58_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676603.1"
                     /translation="MIGITGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIP
                     ANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEINDTLKIVGLYEQKDQKVKTYS
                     LGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHS
                     EEDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1120 a          837 c          696 g         1177 t
ORIGIN      
        1 cctccttaat aagtaagcgt ttactttgtg tattacaaaa atatcttaac tctttttcaa
       61 aacttttaaa atattttgtt tggaaatttc agaaatttta tgtctatgat aaaaatcctt
      121 ataacggcaa taaaaaatag atattatcca aagaagattt taagtgctac aataactgta
      181 ttatttctag atgggaggtt ctatttttgg attgatccat tgttgaacaa tatctaccac
      241 tatatcaaaa ggcattcttt ctgaccttgc atattgcagt ttggggaatt ttgggatcct
      301 ttctgctcgg tttaatcgtt agtatcatcc gacattatcg aatccttgtt ttgtcgcaag
      361 tagcgacagc ctacattgaa ttgtcacgta atacgcccct tttgattcaa ctcttctttc
      421 tctacttcgg tcttccccga atcgggattg tcctatcttc agaagtctgt gcaacgcttg
      481 ggcttgtctt tttaggagac tcctatatgg cagaatcttt ccgaagtggg ctggaagcca
      541 tcagtcaaac ccagcaggag attggcctcg ctattggtct gacacctcta caggtctttt
      601 actatgtggt tcttccgcaa gcaacagcgg tggcactccc ctcctttagt gccaatgtca
      661 ttttccttat caaggaaacc tctgttttct cagcagtggc tttggccgac ctcatgtacg
      721 tcgccaagga tttgattggt ctctactatg agacagacat tgcgctagct atgttggtag
      781 ttgcttatct gatcatgctg ctacccatct cactggtctt tagctggata gaaaggaggc
      841 tccgccatgc aggattcggg aatccaagta ctctttcaag gaaataatct cctgagaatc
      901 ttacagggat tgggcgttac gattgggata tccatcctgt ctgtcctctt atccatgatg
      961 ttcggaacag tcatgggaat catcatgacc tcccattcta gaatcatacg atttttaaca
     1021 cgattgtatc tggaatttat ccgtatcatg ccccagctgg tgctactctt catcgtttac
     1081 tttggcttgg ctcgaaactt taatatcaat atctcaggtg agacttcagc tattatcgtt
     1141 tttaccctct ggggaacagc tgaaatggga gacttggtac gtggagctat cacttctctc
     1201 cctaaacatc agtttgaaag tggacaggca ctcggcttga ctaatgttca actttactac
     1261 cacatcatca tcccacaagt cttgagaaga ctgctaccgc aggctatcaa tcttgtcact
     1321 cggatgatta aaaccacttc attagttgtt ttgattgggg ttgtggaagt gaccaaagtt
     1381 ggacaacaaa tcatcgatag caatcgcctg accatcccaa ctgcttcatt ttggatttat
     1441 ggaaccattc tagtcttata tttcgcagtt tgctacccta tttccaaact atccactcac
     1501 ttagaaaaac attggagaaa ctaaatgtct gaaactatct tagaaatcaa ggaactaaaa
     1561 aaatccttcg gagacaatcc catcctccaa ggactttctc tagaaatcaa aaaaggggaa
     1621 gttgttgtca tcctagggcc atctggttgt gggaaaagta ccctccttcg ttgcctcaac
     1681 ggcttagaaa gtattcaagg tggagatatt tttctggatg gtcagtctat cgttgaaaat
     1741 aaaaaaaatt ttcacctagt tcgccaaaag attggcatgg tctttcaaag ttatgaactc
     1801 tttccccatc tggatgtctt acaaaacctc atcctaggcc ctatcaaaac tcaaggaagg
     1861 gacaagaaag aagtaacgga agaagctttg caattactag atcgtgtcgg tttgctggat
     1921 aaacaacata gctttgcccg tcaattatct ggtggacaga agcaacgtgt tgcaattgtc
     1981 cgtgccctcc taatgcatcc agaaatcatc ctttttgacg aggtgactgc ttcgctggat
     2041 ccagaaatgg tgcgtgaggt gctggaactt atcaatgatt tggcccaaga aggccgtacc
     2101 atgattttag taacccacga aatgcagttt gcccaagcca ttgctgaccg gattatcttc
     2161 ctcgaccaag ggaaaatcgc tgaagaagga acagctcaag ccttctttac caatccgcaa
     2221 accaaacgag cccaggaatt tttaaacgtc tttgacttta gccaatttgg ctcatatcta
     2281 taaaggagat tcttatgaaa ctattcaaac cactcttaac tgttttagca cttgcctttg
     2341 cccttatctt tatcactgct tgtagctcag gtggaaacgc tggttcatcc tctggaaaaa
     2401 caactgccaa agctcgcact atcgatgaaa tcaaaaaaag gcggtgaact gcgaatcgcc
     2461 gtgtttggag ataaaaaacc gtttggctac gttgacaatg atggttctta ccaaggctac
     2521 gatattgaac tagggaacca actagctaaa gaccttggtg tcaaggttaa atacatttca
     2581 gtcgatgctg ccaaccgtgc ggaatacttg atttcaaaca aggtagatat tactcttgct
     2641 aactttacag taactgacga acgtaagaaa caagttgatt ttgcccttcc atatatgaaa
     2701 gtttctctgg gtgtcgtatc acctaagact ggtctcatta cagacgtcaa acaacttgaa
     2761 ggtaaaacct taattgtcac aaaaggaacg actgctgaga cttattttga aaagaatcat
     2821 ccagaaatca aactccaaaa atacgaccaa tacagtgact cttaccaagc tcttcttgac
     2881 ggacgtggag atgccttttc aactgacaat acggaagttc tagcttgggc gcttgaaaat
     2941 aaaggatttg aagtaggaat tacttccctc ggtgatcccg ataccattgc ggcagcagtt
     3001 caaaaaggca accaagaatt gctagacttc atcaataaag atattgaaaa attaggcaag
     3061 gaaaacttct tccacaaggc ctatgaaaaa acacttcacc caacctacgg tgacgctgct
     3121 aaagcagatg acctggttgt tgaaggtggc cattaactct taaaagggac tggattttaa
     3181 gctccaatcc ctttttaaga ttttacctat aacatcctga gtctatctaa gatgttcaat
     3241 ctgaacacag tgtacatact ttatcttcta ttgcatatac tttatcacat aagatacgaa
     3301 tatcctcttc actatgacta gcaatcaaaa ttgttgtccc tttttcacta gagagctttc
     3361 taaacaatgt tctcatattt tctacacttg atttatccaa ggcattcata ggttcatcta
     3421 gtaaaagaat agagggattc tccataattg cttgagcaat ccctagcttt ttcctcatac
     3481 ctagcgaata agttttaact ttctggtctt tttgctcata tagaccaact attttcagtg
     3541 tatcattgat ttcctgatta ccaactactc ctcgtatgct tgccaaatat tgtaaattct
     3601 taaagccact ataataattt ataaaaccag gttcttcaat caaagctccc aaattagctg
     3661 gaatttttct ctcaggaaca atattttccc cattgattaa cacttctcca taagacggac
     3721 tatataaacc agctattaat ttaaacaata cacttttccc tgagccattc gcaccagtaa
     3781 ttcctataat ttccccctgt ttacaactaa aattaaggtt ttgaaaaaca
//
LOCUS       DAVLFB010000131         3739 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLFB010000131 DAVLFB010000000
VERSION     DAVLFB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.131"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            127..1185
                     /locus_tag="VXD58_001969"
     CDS             127..1185
                     /locus_tag="VXD58_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV0676604.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            1336..1701
                     /locus_tag="VXD58_001970"
     CDS             1336..1701
                     /locus_tag="VXD58_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV0676605.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            1777..2718
                     /locus_tag="VXD58_001971"
     CDS             1777..2718
                     /locus_tag="VXD58_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV0676606.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            2666..3682
                     /locus_tag="VXD58_001972"
     CDS             2666..3682
                     /locus_tag="VXD58_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV0676607.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
BASE COUNT         1132 a          649 c          860 g         1098 t
ORIGIN      
        1 tttatacagg gtactttact ggtaaataaa aagttaaaaa aatcacaaaa aaagcttgaa
       61 gaaacaaaga aaaaggctta caattgaact ataaatatta caaataaaaa aacggaggag
      121 tgctttatga aagcctatac ttatgttaaa ccaggacttg cttcttttgt tgatgtagac
      181 aaaccagtta ttcgcaagcc aacagacgct attgtgcgta ttgtaaaaac cactatttgt
      241 ggaacagacc tccatattat caaaggggat gttcctactt gccaaagtgg taccattctt
      301 ggccacgaag ggattgggat tgttgaagaa gttggggaag gagtttccaa cttcaaaaaa
      361 ggcgacaagg tcttgatttc ttgcgtctgt gcctgtggta aatgctacta ctgtaaaaaa
      421 ggaatttatg cccactgtga agacgaaggg ggctggattt tcggtcactt gattgatggt
      481 atgcaggctg aatatctacg tgtccctcat gcagataata ctctttacca tactccagaa
      541 gacttgtcag atgaagcttt ggttatgctg tcagacattc tgcctactgg atatgaaatt
      601 ggtgtcttaa aagggaaagt agaacctggt tgcagcgtag ccattattgg ttcaggtcca
      661 gttggattgg ctgctctttt aacagcccaa ttctattcac cagctaaatt gattatggta
      721 gacctagacg ataaccgctt ggaaactgcc ctatcattcg gtgcgactca taaggttaat
      781 tcttcagacc ctgaaaaagc cattaaagaa atttatgatt tgacagatgg tcgtggtgtg
      841 gatgtcgcta tcgaagctgt tggtattcct gcaacatttg atttctgtca aaagattatc
      901 ggtgtagacg gaacggttgc caactgtggt gtgcatggta aaccagttga attcgattta
      961 gataaacttt ggattcgcaa catcaatgta acaactggtt tggtatctac aaatacgact
     1021 ccacaattgt tgaaagcact tgaaagtcat aagattgaac cagaaaaatt ggtaactcac
     1081 tatttcaaac tcagtgaaat tgaaaaagcc tatgaagtct tcagtaaggc agcagaccac
     1141 catgccatta aggtcattat cgaaaacgat atctcagaag cctaagtagt aaaaatattt
     1201 ttgtacataa gtaaatagaa attcagtcat ccatcagatg gctggatttt ttatcaaaaa
     1261 attaagaaat gagcatattt ctttccttgt ctggcggaat tggttataat atacggtaca
     1321 aaggaatgaa tgaatatgta tcgtgttata gaaatgtacg gagattttga accgtggtgg
     1381 ttcttagaag gttgggaaga agatattgta gcaagtagaa aatttgacca gtattatgat
     1441 gctctcaaat actacaaaac ttgctggttt agattggaac aagaatcccc tctttataaa
     1501 agtagaagcg acttgatgac cattttttgg gacccggaag accaacgctg gtgtgatgaa
     1561 tgtgatgagt atttacaaca ataccattct ttggctcttt tgcaggatga gcaggttatc
     1621 ccagacgaaa aactacgctc aggctatgaa aaacaaacca gtcaggaaag gaatcgttct
     1681 tgccgtatga aattaaaata gagaaaagta acttttttgg agttgctttt tttatttttc
     1741 taacgctttg cgaatagtat aggtgaggag gtaagtatgg ttcaagaaat tgcacaagaa
     1801 atcattcgtt cagctcggaa aaaagggacg caggatattt attttgtccc taagttagac
     1861 gcctatgagc ttcatatgag ggtaggagac gagcgctgta aaattggtag ctatgatttt
     1921 gaaaagtttg cagccgttat cagtcacttt aagtttgtgg cgggtatgaa tgtgggagaa
     1981 aaaagacgta gtcaactggg ttcctgtgat tatgcctatg accagaagat ggcgtctcta
     2041 cgtttatcta ctgtaggcga ttatcggggg catgagagtt tggttatccg tttgttgcac
     2101 gatgaggagc aggacttgca tttttggttt caggatattg aagaattagg caagcagtac
     2161 aggcaacggg gactctatct ttttgctggt ccggttggga gtggtaagac gaccttgatg
     2221 catgaattgt ccaagtcact ctttaaagga cagcaagtta tgtccatcga agatcctgtc
     2281 gaaatcaagc aggacgacat gcttcagttg cagttgaacg aagcaatcgg cctaacctat
     2341 gaaaatctaa tcaaactttc cttgcgtcat cgaccagatc tcttgattat cggagaaatt
     2401 cgtgacagcg agacggcgcg tgcagtggtc agagctagtt tgacaggtgc gacagtcttt
     2461 tcaaccattc acgccaagag tatccgaggt gtttatgagc gtctgctgga gttgggtgtg
     2521 agtgaagaag aattggcagt tgttctgcaa ggagtctgct accagagatt aatcggggga
     2581 ggaggaatcg ttgactttgc aagcagagat tatcaagaac accaagcagc caagtggaat
     2641 gagcaaattg accagcttct taaagatgga catatcacaa gtcttcaggc tgagacggaa
     2701 aaaattagct acagctaagc aaaaaaatat catcacccta tttaacaatc tcttttctag
     2761 cggttttcat ctggtggaga ctatctcctt tttagatagg agtgctttgt tggacaagca
     2821 gtgtgtgatc cagatgcgtg cgggcttgtc tcaggggaaa tcattctcag aaatgatgga
     2881 aagtttggga tgttcaagta ccattgtcac tcagttatct ctagctgaag ttcatggcaa
     2941 tcttcacctg agtttgggaa agatagaaga atatctagac aatctggcca aggtcaagaa
     3001 aaagttaatt gaagtagcga cctatccttt gattttgctg ggttttcttc tcttaattat
     3061 gctggggcta cggaattacc tgctcccaca actggatagt agcaatattg ccacccgaat
     3121 tatcggtaat ctgccacaaa tttttctagg catggtaggg cttgtttccg tgcttgccct
     3181 tttagcacta actttttata aaagaagttc taagatgagt gtcttttcta tcttagcacg
     3241 ccttcccttt attggaatct ttgtgcagac ctacttgaca gcctattatg cacgtgaatg
     3301 ggggaatatg atttcacagg gaatggagct gacgcagatt tttcaaatga tgcaggaaca
     3361 aggttcccag ctctttaaag aaatcggtca agatctggct caaaccctga aaaatggccg
     3421 tgaattttct cagacgatag gaacctatcc tttctttagg aaggaattga gtctcatcat
     3481 agagtatggg gaagttaagt ccaagctggg tagtgagttg gaaatctatg ctgaaaaaac
     3541 ttgggaagcc ttttttaccc gagtcaaccg caccatgaat ttggtgcagc cactggtttt
     3601 tatctttgtg gcactgatta tcgttttact ttatgcggca atgctcatgc ccatgtatca
     3661 aaatatggag gtaaattttt aaaatgaaaa aaatgatgac attcttgaaa aaagctaagg
     3721 ttaaagcttt tacattggt
//
LOCUS       DAVLFB010000132         3715 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLFB010000132 DAVLFB010000000
VERSION     DAVLFB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.132"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            104..331
                     /locus_tag="VXD58_001973"
     CDS             104..331
                     /locus_tag="VXD58_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676608.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            619..3516
                     /gene="nanA"
                     /locus_tag="VXD58_001974"
     CDS             619..3516
                     /gene="nanA"
                     /locus_tag="VXD58_001974"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV0676609.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKDPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1268 a          629 c          842 g          976 t
ORIGIN      
        1 ctatttttat gtatatttaa agatagattg ctgtaaaaat agtagtagct atgcgaaata
       61 acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat ttaaggcatt
      121 caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt ttttcgacag
      181 ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc tggataagat
      241 tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg cttatgaaaa
      301 gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtgtaaa tagcataggc
      361 tgatgtccat catttgctta taaagagata ttttagttta attgcagcgg tgtcctggta
      421 gataaactag attggcagga gtctgattgg agaaaggaga ggggaaaatt ggcaccaatt
      481 tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa agaaagttaa
      541 taaaagacaa actaagtgca ttttctggag taaatgtctt atttcagaaa tcgggatata
      601 gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg ttatagcatt
      661 aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt tggaacgtct
      721 cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac tcaactttcg
      781 ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga gactgaactt
      841 tctggcaata atcaagaaca agaaaggaaa gataagcaag aagaaaaaat tccaagagat
      901 tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga tgttgaaacc
      961 aatgcttcaa atggtcagag agttgattta tcaagtgaac tagataaact aaagaaactt
     1021 gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggccccagc attctataat
     1081 ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc agtttacaat
     1141 aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacaa taattacaac
     1201 gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac agttgaaaaa
     1261 ccgacagcag aactacctaa aggccgagtg cgcctctacg taaacggggt attatctcga
     1321 acaagtctga gatctggcaa tttcattaaa gatatgccag atgtaacgca tgtgcaaatc
     1381 ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat tcggaatctc
     1441 actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca actttttaaa
     1501 cgctcagatt tagaaaaaaa actacctgaa ggagcggctt taacagagaa aacggacata
     1561 ttcgaaagcg ggcgtaacgg taacccaaat aaagatggaa tcaagagtta tcgtattcca
     1621 gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg ccgtctccat
     1681 tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa tggtaaaact
     1741 tggggtgacc gagtaaccat taccaactta cgtgacaatc caaaagcttc tgacccatcg
     1801 atcggttcac cagtgaatat cgatatggtg ttggttcaag atcctgaaac caaacgaatc
     1861 ttttctatct atgacatgtt cccagaaggg aagggaatct ttggaatgtc ttcacaaaaa
     1921 gaagaagcct acaaaaaaat cgatggaaaa acctatcaaa tcctctaccg tgaaggagaa
     1981 aagggagctt ataccattcg agaaaatggt actgtctata caccagatgg taaggcgaca
     2041 gactatcgcg ttgttgtaga tcctgttaaa ccagcctata gcgacaaggg tgatctatac
     2101 aagggtaacc aattactagg caatatctac ttcacaacaa acaaaacttc tccatttaga
     2161 attgccaagg atagctatct atggatgtcc tacagtgatg acgacgggaa gacatggtca
     2221 gcgcctcaag atattactcc gatggtcaaa gccgattgga tgaaattctt gggtgtaggt
     2281 cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt gataccggtt
     2341 tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt catctattca
     2401 gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg tcaggtagac
     2461 ggtcaaaaga tccactcttc tacgatgaac aataaacgtg cgcaaaatac agaatcaacg
     2521 gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac tggagatctt
     2581 caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa acgttatcca
     2641 caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcacga aggaaaagaa
     2701 tacatcatcc tcagtaatgc aggtggaccg aaacgtgaaa atgggatggt ccacttggca
     2761 cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca aaaaggagag
     2821 tttgcctata attcgctcca agaattagga aatggggagt atggcatctt gtatgaacat
     2881 actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg ggactttttg
     2941 agcaaagatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga gatgggcaaa
     3001 ggagagatgg gcaaaggagt tattggcttg gagttcgact cagaagtatt ggtcaacaag
     3061 gatccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca gtatgatagc
     3121 aagaccttgt tatttgcagt agataaggaa gatatcggac aggaaattat tggtatagct
     3181 aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc cagagttcct
     3241 ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac agggggagtt
     3301 aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga ttcgcttgta
     3361 actcttacta caaaagaaga ttatacttac aaagctcctc ttgctcagca ggcacttcct
     3421 gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc tttcttcctt
     3481 ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa acatttgatt
     3541 ttgtaaaaat agaaggagat agcaggtttt caagcctgct atcttttttt gatgacattc
     3601 aagctgatac gaaatcataa gaggtctgaa actactttca gggtagtctg ttctataaaa
     3661 tatagtagat tgaaataaga tgtgaacaac tctaccagga aagtcaaatt aattt
//
LOCUS       DAVLFB010000133         3705 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLFB010000133 DAVLFB010000000
VERSION     DAVLFB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.133"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..250
                     /locus_tag="VXD58_001975"
     CDS             <1..250
                     /locus_tag="VXD58_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676610.1"
                     /translation="FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIVYLFLG
                     YLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELFKNSK"
     gene            1385..1888
                     /locus_tag="VXD58_001976"
     CDS             1385..1888
                     /locus_tag="VXD58_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676611.1"
                     /translation="MKLLDCILDYQERFNRKACQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILLYNDVIERISLYEFIGDIFYFKITSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            2174..>3705
                     /locus_tag="VXD58_001977"
     CDS             2174..>3705
                     /locus_tag="VXD58_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEV0676612.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEEKAKKAEEAAKEVDDASLA
                     VQKAYVEQRKAQEEKNKHSGNPEYAKKLAEADKKITEETTKLTDAQTKFQSVQAMVVP
                     EPEQLAETKKKAEEAKAEEKVAKRKYDYATLKVVLAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAA
                     LQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPAPKPEQPAP
                     APAPKPEQPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYL
                     NANGSMATGWVKDGDTWYYLEASGAMKASYWFKVSDKWYYVNSNGAMATGWLQYNGSW
                     YYL"
BASE COUNT         1349 a          541 c          738 g         1077 t
ORIGIN      
        1 ctttttgata aaaatttgtg tagatactac gataaaattt ttattcatca gattatttga
       61 cacaagtact ttaatgaaga ggagaatatt ttttcttttc gcattggatg ttatagttta
      121 cttattttta ggatatcttt tagtgattca aaaagcaggc tatttgtttt cggttttact
      181 tcttttttct aatttttcag ttccatttat caaagaaaag gaattcgaat tatttaaaaa
      241 tagcaagtaa tagaaacata ggttaaatct tagaacggct taaaatcaga tatgataaag
      301 ggaaatagtc ttatctaggt aactaagtgc tacaaacaca gtcgaggatc acttgttagg
      361 attacatttt aatcatgctt aataagaatg gagaattcct atggggattg tattactctg
      421 aagtttcgaa taccttagtt ctcgtgattt tgagctgttc tagctttaga atccttcaga
      481 acgtaaaatt ttaagcagtc aaagacttag aatagcctgg aaacatttat tttatagcaa
      541 ttctaaactt agaattgctc atatatatgt atttgagctg ctcgatagtt aggacagctc
      601 tattctgaga aatctgagct atcaatgctt cagaatagta tttcgccaat ggtttttatt
      661 gaagaggtga ttgtgttatg atagtctaaa ataaaattga tttaatagag taaatggaga
      721 atcgttacat gaaacatttt tttgcaggaa ttggtgagat aaaacttgtt ggctacttat
      781 tgtatatatg tgtaggacta gcccctcttt ttcatatata tgtcataggt tcggaactga
      841 gttttgtgaa aatagtgtta tctattttgg gagttatatt tgtaagtatg ttaactattg
      901 ctcgaattta tcggactttt ctataaagaa tagtttggac ttgaactgta tctaaaaaca
      961 gaggttggtc gattatcagc ctctttcttg tcgttgaaaa gagtgagata tattgacttt
     1021 tgaaaaaaag atgtcataat tgagttggga taaacctaaa agtaatatag tagattgaat
     1081 ctggaatagt ccgttatgac tgttaaaata tttctagaaa ttaatttgac tttcctaatc
     1141 gatttgttca catcttgttt caatctacta tactttctcg ttaaaccgtt cggcaattca
     1201 tggtgacatg gacacatagt agtaagcaca ctatgtggct tggcagagct aaaaactgtt
     1261 cccttgcgat aagcctaata agcacaacat agggagttag agaagccgac tctaatcaac
     1321 cactttgggc agtagtgaga actgccccgt gcttttttat tttgagaaaa tatggagttt
     1381 gtcgttgaaa ttacttgatt gtattttgga ttatcaagaa agattcaata gaaaagcatg
     1441 tcaagtatca acgaattata agtatttaga gactttcaaa gtgaattttt gcctgactga
     1501 tttacatcat ttatttggct tacacaaaat cacacgagat tatgctagtc aaacaaaacc
     1561 tgctattcaa gctggtgttt ttattttaga agattttaga aatatcctat tgtacaatga
     1621 tgtaatcgaa aggatatctt tatatgagtt tataggtgat attttctatt ttaaaataac
     1681 aagttgttgc atcgtagcta aggatttatc taaaaatact atgaaattgg acgtcatatt
     1741 ttttgaggat aaaaataaaa gatccgcagt tttaggttta cgaagagaca aaagcggagt
     1801 atttaaacca gttactctac attttacaag cgctaagaaa tatgctaaag ttcgtaaaac
     1861 agatgtgaaa gaaatgaaat ggctataaag attgtccgca ggcttaagct tgcgtttttt
     1921 gttgtttaac gaaattaaaa aacaagctct ctcatcggaa atgtttttat aggaatgaag
     1981 gaagatgatg ctatgtttaa aaagagaatc gaaaagggca aaagtagtgt ctttatcttt
     2041 ttggagtaga agcttatgat atagaaattt gtaataaaaa tgtaatataa aacatttgac
     2101 aaatatttac ggggggagta tacttaatat aagtatagtc taaaaatgac tatcagaaaa
     2161 gaggtaaatt tagatgaata agaaaaaaat gattttaaca agtctagcca gcgtcgctat
     2221 cttaggggct ggttttgtta cgtctcagcc tacttttgta agagcagaag aagctccaca
     2281 agttgtcgaa aaatcttcat tagagaagaa atatgaggaa gcaaaagcaa aagctgatac
     2341 tgccaagaaa gattacgaaa cggctaaaaa gaaagcagaa gacgctcaga agaaatatga
     2401 tgaggatcag aagaaaactg aagagaaagc gaaaaaagca gaagaagctg ctaaggaagt
     2461 agatgatgct agtttagcgg tgcaaaaagc ttatgtggaa caaagaaaag cacaagaaga
     2521 aaaaaataaa cattcaggaa atccagagta cgctaaaaaa ttagcagaag cagataaaaa
     2581 aataactgag gagacgacta aactaaccga tgctcagaca aaatttcaat ctgttcaagc
     2641 aatggtagtt cctgagccag agcagttggc tgagactaag aaaaaagcag aagaagctaa
     2701 agcagaagaa aaagtagcta agagaaaata tgattatgca actctaaagg tagtactagc
     2761 gaagaaagaa gtagaggcta aggaacttga aattgaaaaa cttcaatatg aaatttctac
     2821 tttggaacaa gaagttgcta ctgctcaaca tcaagtagat aatttgaaaa aacttcttgc
     2881 tggtgcggat cctgatgatg gcacagaagt tatagaagct aaattaaaaa aaggagaagc
     2941 tgagctaaac gctaaacaag ctgagttagc aaaaaaacaa acagaacttg aaaaacttct
     3001 tgacagcctt gatcctgaag gtaagactca ggatgaatta gataaagaag cagaagaagc
     3061 tgagttggat aaaaaagctg atgaacttca aaataaagtt gctgatttag aaaaagaaat
     3121 tagtaacctt gaaatattac ttggaggggc tgattctgaa gatgatactg ctgctcttca
     3181 aaataaatta gctactaaaa aagctgaatt ggaaaaaact caaaaagaat tagatgcagc
     3241 tcttaatgag ttaggccctg atggagatga agaagaaact ccagcgccgg ctcctcaacc
     3301 agagcaacca gctccagctc ctgcaccaaa accagagcaa ccagctccag ctcctgcacc
     3361 aaaaccagag caaccagctc cagctcctgc accaaaacca gagcaaccag ctcccgcacc
     3421 aaaaacaggc tggaaacaag aaaacggtat gtggtacttc tacaatactg atggttcaat
     3481 ggcgacagga tggctccaaa acaacggttc atggtactac ctaaacgcta acggttcaat
     3541 ggcaacaggt tgggtgaaag atggagatac ctggtactat cttgaagcat caggtgctat
     3601 gaaagcaagc tattggttca aagtatcaga taaatggtac tatgtcaaca gcaatggcgc
     3661 tatggcgaca ggatggctcc aatacaatgg ttcatggtac tacct
//
LOCUS       DAVLFB010000134         3683 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLFB010000134 DAVLFB010000000
VERSION     DAVLFB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.134"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            12..1421
                     /locus_tag="VXD58_001978"
     CDS             12..1421
                     /locus_tag="VXD58_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0676613.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1439..2734
                     /locus_tag="VXD58_001979"
     CDS             1439..2734
                     /locus_tag="VXD58_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV0676614.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2739..3047
                     /locus_tag="VXD58_001980"
     CDS             2739..3047
                     /locus_tag="VXD58_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV0676615.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3044..3352
                     /locus_tag="VXD58_001981"
     CDS             3044..3352
                     /locus_tag="VXD58_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV0676616.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1235 a          622 c          710 g         1116 t
ORIGIN      
        1 ggaggttgag catgaaaaaa aagttagtat ttcctaatct gttttggtgg ggagcagctt
       61 ctagcggacc tcagacagaa ggtcaatatg gaaaagtaca tgaaaatgtg atggactact
      121 ggttcaaaac gcatccagaa gattttttcg ataatgtcgg acctcttgta gccagtaact
      181 tttttcatac ttacaccgaa gatttccact tgatgaagga aattggagtt aattctttcc
      241 gcacttccat ccaatggagt cgactcatca agaatttaga gacaggtgag cctgatccaa
      301 aaggtattgc tttctacaat gccatcattg aagaagctaa aaagaaccag atggatcttg
      361 tgatgaattt acatcatttt gatttaccag tggaacttct tcaaaaatac ggtggttggg
      421 aaagcaaaca tgtagtggag ttattcgtga agtttgccaa gactgctttc acatgctttg
      481 gagataaggt tcattactgg acaactttca atgagccaat ggtcattcca gaagcaggat
      541 acttatatgc tttccattat ccaaatctaa aaggaaaggg aaaagaggcc gtacaagtca
      601 tctataatct aaaccttgct agtgcaaaag tgattcaact atatcgctca ttagaacttg
      661 atggaaagat tgggattatt ttaaacttga cacctgctta tccaagaagt aattctccag
      721 aagacttaga agcaagtcga tttacagatg acttctttaa caaagtcttc ttgaatccag
      781 ctgttaaagg aactttccca gaaagattgg taaaacagct agagagagat ggcgtgttat
      841 ggagtcatac cgaaaaagag cttcaactga tgaaatcaaa tacggttgat tttcttggag
      901 taaactacta ccatccaaaa cgtgttcaag cacaagcaaa tcctgaggaa tatcagacgc
      961 cctggatgcc agaccaatac ttcaaagagt atgaatggcc ggagcgtcgc atgaatccat
     1021 atcgtggttg ggaaattttt ccgaaatcca tttatgatat tgctatgatt gtgaaggaag
     1081 aatatggtaa tatcccatgg tttatcagtg aaaacggaat gggtgttgaa aacgaagcac
     1141 ggtttatcga tgaaaatgga gttatcgatg acgtgtatcg tattgaattt tatgaagaac
     1201 atttaagatg gctacataaa gccattgaag agggaagtca ctgttttgga taccacgctt
     1261 ggaccgcatt tgattgctgg tcttggaata atgcatataa gaatcgttac ggatttatct
     1321 ccgttgattt agaaacgcaa aagagaacca tcaagagctc aggaagatgg tatcgcaaag
     1381 taagtgacaa taacggtttt gaagtagaaa ttgaggagta aagagaggag atattcatat
     1441 gaaaaactca aaatttatag accaatttgc cacctttgct ggtaaactag ggaaccaaat
     1501 tcatttaaaa accctaagag atgcattcgt aacagtaatg ccattatata ttttggcagg
     1561 tttaatcgtt cttttgaaca acacggtatt taagtggatt ttccaagggg atacattaac
     1621 aagattccaa tattggggaa taacaattgc aaacggtact ttaagtattt caggtatgat
     1681 tattgctgta atggttggtt atttcttagc taaaaacaga gatttcgaaa acccgttagc
     1741 agcatcaatg ctatcattag tttctttaat tgtgatgatg ccaaatacag tttctgtagt
     1801 tcctgacgga gcaaaagatg ctgtaaacat ttcaggtgtt ctttcattca acaacacagg
     1861 tacaggcgca atgttcgccg gggttatcgt agcgattatt gcaacagaat tattcattga
     1921 attatcaaat gttaaagctt tacaaatgaa ccttggtgaa aatattccac cagctgttaa
     1981 tagatcattt agcgtattac ttccagtcat gaccgtcatc tccttatttg gggttgtttc
     2041 agcattatta ttcaatataa ctggaatgaa cttaatctca atcattacaa tctttattca
     2101 agaaccaatt cgtcatattg gtacaagctt aatcggggtc attattattt actctttagg
     2161 aaatatgtta tggctatttg gtattcacca agcagttatt tacagtgcca tcctagaacc
     2221 attactatta attaacatta ctgaaaacat cactgcagca aataatggac aagccattcc
     2281 acacatcatc aacctatcac aaatacaaac attcgcttta atgggtggta gtggatctac
     2341 attatgttta ttaatagcaa cattcttagt gagtcgcaat gctgtctcta aaaacgtggc
     2401 taaattatct tttggacctg gtatcttcaa tatcaatgaa ccagtattat tcggttaccc
     2461 aatcgtttat aacatttcat tagctattcc atttatcaca gttccagtcc ttggtatttt
     2521 aatcagctac ttagcaacag ttacaggatt catgagtcct gcatttatac aagttccttg
     2581 gactacacca gtattcttaa atgcatggtt agcaacagca ggggacgtga gagcagttct
     2641 agttcaattc atcatctttg cacttggagt tcttctatac attccattta tcaaagttaa
     2701 tgacaaagtt gttgaacaag aaatggaagg ttaagattat gaaacaaatt ttattagtat
     2761 gtaatgcggg aatgtcgaca agtatgcttg ttaaaaaaat gcaacaaagt gcgactgaac
     2821 gtgggattga aatctctatc caagcaaaat caatgacaga agctaaaaag aatatccatg
     2881 aagcagatgt cattctaatt gggccacaaa ttcgttatga gttacttgct gtgaaagaaa
     2941 ttgcaggaaa tatccctgta gataccattg atatgcggga ttacggaatg atgaatggag
     3001 caaaagttct ggaacaagca cttgcatgga taggagaaat aagatgacag aagaaatgga
     3061 atatatctgt tttcagctca ttgccaatag tggagcagcc aaatcatcct ttattgaagc
     3121 catccaacta gctaaggcag gaaatttaaa agaagcgaaa attaaagttg aagaagcgga
     3181 agactcctta gtagaagcgc acaagattca ctctaatctc atacaaaaag aagctactgg
     3241 agaaaaaata ggattctcct tgctatttat gcatgcagaa gaccaaatgg cttcaacaga
     3301 gattattcag ttgctatcta aagaattcat cgatctctat caaaataaat aggaacactg
     3361 tcattcccat ctgtctttat taatgacgac tacattgtag gaacctccaa aagttggaca
     3421 aaatgattga aaggattgga tcctgcatta cacaagatcc aatccttttc ttttactcta
     3481 gtttttataa tatgttgaat ctagaatagt ccactgtagt ttctaaaaaa ttgctggaaa
     3541 tggatgttaa gctccaattt atttgtttat atcttatttc agtttactat actagcttat
     3601 tattctagtg aaaaattgaa tgagccacta tttatttaat atgtatgatg agggaatgac
     3661 tcaaaaactt cttgtcgata taa
//
LOCUS       DAVLFB010000135         3635 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLFB010000135 DAVLFB010000000
VERSION     DAVLFB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.135"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(76..1491)
                     /locus_tag="VXD58_001982"
     CDS             complement(76..1491)
                     /locus_tag="VXD58_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV0676617.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1504..3342)
                     /locus_tag="VXD58_001983"
     CDS             complement(1504..3342)
                     /locus_tag="VXD58_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV0676618.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3360..>3635)
                     /locus_tag="VXD58_001984"
     CDS             complement(3360..>3635)
                     /locus_tag="VXD58_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV0676619.1"
                     /translation="FYCTEFNQDSIDYYRFITHIKLFAHRLVENTTYCDDDDEDLLAL
                     MKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1221 a          741 c          482 g         1191 t
ORIGIN      
        1 cttccaaacc tctttatttc atatttaatg agatgctgcc ttaaccgtga taatatctaa
       61 tcatcaataa acaaactatt tgaataagga ttctccattt gattcaatca ctcctttata
      121 ccaagtaaag gacattttct tatatcgatt gaatgtgcca tttccatcat catttcgatc
      181 aacataaatg agaccgtacc tcttagaaag ctgtgcagtg gacatagaaa cgcagtcaat
      241 acatccccaa gacgtatagc ccataatttc cacaccatct tgtagagctt cagctacttg
      301 caataaatgt tctttcatat actgaattct ataatcatct tggactgtta agttattaaa
      361 ttcatctttt attagttgat ctttagcacc taatccgttt tccactataa ataatggaat
      421 ttgataacgg tcataatatc tatttaaaat tatacgtagt ccaattggat caatttgcca
      481 tccccactca gaagactcta aataaggatt tactaaacca ccaataatat tcccttctcc
      541 tgaattatac tgtgttggaa gagcagattg agtcacactc atgtaatagc taaaggataa
      601 aaaatctaca gtataatttt ttaataactc tgcatcttca gctgcaaact ctatgttaat
      661 gtcattttcc ttaaaatatc tttttgcata attcggataa taacctctaa catgcacatc
      721 tgaaaataga taatttagat tctcatactc atgagtcgcc catacatctt ttggatttgg
      781 agtcattgga taagctggca tagctaatac catacacccc accttaaact ctgaattaat
      841 ctcacgagca atttttgtaa ccaaactcga ggcgactaat tcatgatgta tagcttgata
      901 taattcttgt ttcgaaagat tctccttagg tatatctatt cctccactag taaacggtaa
      961 ttccaaaaca gagtttactt cgttaaatgt aagccaatat ttaactttat ctttatacct
     1021 ttctaaaact gttcgagcaa atttttcata aaaatgaatc attctcctat caatccatcc
     1081 atgatatttt cttgctaaat ataatggagt ctcatagtgt gaaagagtta caagtggttc
     1141 tatcccgtga gcatgtagtt catcaaacaa ttcatcataa tatttcaacc cagcttcgtt
     1201 aggttcttcc tcatctcctt ttggaaaaat tctactccat gcaatagaag tacgaaaaac
     1261 attaaagccc atttcagaaa acaaggatat atcttcctta tatttatgat aaaaatcaat
     1321 acctatcaat tttaagttat cttctgtagg attttctgtt gcttctccta atccaccttt
     1381 gggtaacaca tcctgaactg ataagccctt accatcttca ttatatgctc cctctacttg
     1441 attagctgca acagctccac cccaaagaaa atcatctgga aaaatggtca taactttcct
     1501 ccattataat attaccagta attccttaga atgctcgatt gtctgattat taggtaatac
     1561 taatacatct agaaaatcat tggtattcgt tacaattact ggtgtaactg tttcgtagcc
     1621 tttagtcttg attaaattca agtccatttc aaaaatcaac tgatttttga aaactctgtc
     1681 tccttcttct acatgactaa taaaaccttg accttttagc tcaacagtat ctaatccaat
     1741 atgaattagt aactcaacac cctcatcact cttcaatcca attgcgtgct tagtcggaaa
     1801 aatatttgta attttcccat caaatggtgc ataaacctta ccttcacttg gaataatcgc
     1861 tactccgtct ccaattagtt tatctgaaaa tgttttatcc tggacatcgc ttaacggaat
     1921 gatttctcct gatataggag aaaatatcat ttttttattt gaaactccag ctttaacttc
     1981 taaattgcta gaactctctt cttcatcgat tccaaatata taagctaata caaaggtaat
     2041 aacaaccgaa ataaccgcca caattaaagc atttacaata tttgatggca catcagaata
     2101 aataaattga ggcaacgcta tcaaagatgg gacagcaaat agatacgctt taacactagt
     2161 aagacctgca aataatcccg ctaatccacc accaatcata gctgcataaa gcggtttttt
     2221 atattttaaa gtcacaccat ataatgcagg ttcggtaatc cctgcaagta aggctgagaa
     2281 acctgctgca aaagcaattt gttttgtatt attattttta ctctttaatg caacagccat
     2341 cgaagcagcc ccttgagcta agtttgaccc taacattgct ggaagaatta atacgtctgg
     2401 agtagcaata gatgccgcca aaaaaatagg tgcaaaagcc caatgcattc cagtcataac
     2461 aataaatggc ataatagcac caagaatagc taatgtaagc catccagcta caccatacat
     2521 ttgcccaact agatttgata atccttcacc aacaattact ccaataggcc cgactacaac
     2581 taaggcaata cagcttgata ctaataatac tagcgtaggt tgcaaaaaac tcttagtaac
     2641 agctggtgtt aatttagcaa tcattttttc aatatatttc atcaaccaaa ccataataag
     2701 aattggaacg actgacgaac cataactagc tggtgtcaca ggtacaccaa ataaactaag
     2761 aggattccct gattgcacca tttgaacaaa atttggatgg agaagtacac ctgctacaga
     2821 catagctaat gtagatgtta cttttaattt ttgtgatgca gaataagcta ataacagcgg
     2881 taagaaataa tatggagcat ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga
     2941 ttgcaatata ccaagcattg gtaaaataat taccaagact ttcagcatac ctccccctaa
     3001 cattgctgga atgattggag tcatggaacc agcgatatac tcaatgattc tttctaaaat
     3061 attccctttg tgcccttgaa caactgaatc ggattcaaaa ttgccaagtt taacgaattc
     3121 tttataataa ttagctacat cattaccaag tataatttga tattgtccat tctttttcat
     3181 aatacctatt acacctggta tcttcttcac atcatcatca ttgactaaat tttcatcttt
     3241 taattctaat cttaaacgtg ttacacaatg ggtaactcta ttgacatttt tttcacctcc
     3301 aattacatcg aggatttttt gtaccgtatc tttataactc atggtattct cctattctat
     3361 taatctaaat tttttgttaa gcgacgaata tgagccatca aataaactaa ttcactagaa
     3421 gtcagcaaat aattgtactc cgtttgtata aacattgcca cctgttcacc acattcatat
     3481 tctctaggat atttattttt cattaatgct aacaagtctt catcatcatc gtcacaataa
     3541 gttgtatttt caaccaagcg atgggcaaat aattttatat gagttatgaa tctataataa
     3601 tcaatagaat cttggttaaa ctcagtacaa tagaa
//
LOCUS       DAVLFB010000136         3622 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLFB010000136 DAVLFB010000000
VERSION     DAVLFB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.136"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            71..871
                     /locus_tag="VXD58_001985"
     CDS             71..871
                     /locus_tag="VXD58_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV0676620.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            966..1808
                     /locus_tag="VXD58_001986"
     CDS             966..1808
                     /locus_tag="VXD58_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV0676621.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            1808..2290
                     /locus_tag="VXD58_001987"
     CDS             1808..2290
                     /locus_tag="VXD58_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV0676622.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            2290..2910
                     /locus_tag="VXD58_001988"
     CDS             2290..2910
                     /locus_tag="VXD58_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV0676623.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(3164..3259)
                     /locus_tag="VXD58_001989"
                     /pseudo
     CDS             complement(3164..3259)
                     /locus_tag="VXD58_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3539..>3622
                     /locus_tag="VXD58_001990"
     CDS             3539..>3622
                     /locus_tag="VXD58_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0676624.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKF"
BASE COUNT         1123 a          717 c          651 g         1131 t
ORIGIN      
        1 aatgctctaa agtctcttcc ttaacccatt tttttgattt tttttgattt ttagggtaga
       61 attataagat atgagaaaac aaattcatcc acttatttta tttagttttt tggggattat
      121 gatgttcatt ttaatcatca atcgcccact taatgacaac caatcgttta aaacaaaatc
      181 caatatagca cagattgaag cacaggcttt gaaacactta gacaaaccga ttattgacct
      241 ctctggctgg cagcgtcctg aagaaatcaa ctacgatgcc ctctcacaaa atatttcagg
      301 tgctattgtt cgtgttcaca gtggtgctca aacgacaaaa gaaaatgatg cttcatttat
      361 caatggaata gacaaggcct ataagagtca tatcactgaa cttcaaaagc ggaatgtccc
      421 agttgctgtc tacgcttatg tagctggaaa aagtgtccaa gaaatggaaa aggctgccga
      481 agttttctac aatgctgcct ctccttacag ccctagttac tattggcttg acgtggaaga
      541 caaaaccatg tctaatatga acgaaggtgt cgaaaacttt cgagcaaaac tagcatcact
      601 aggtgctaaa aacatcggca tctacgttgg tgtctacttc atggaagaac acagtattga
      661 tacaggcaag tttacctctg tttggattcc gtcctacggc tcagactcag gatttttgga
      721 gagcagtcct aaaactgatt tagattatga tattcaccaa tacacatcta aaggaaaaat
      781 tgccggcttt gaccacgatt tagatatcaa cgttatctct cccttaaaaa ataaagaaga
      841 aacctttcga aaactttttt taaaacctta aaagcatttg tttatcattt gctttttctt
      901 tttgtgtctg attgtgatac aatatagtaa ggattttttg aaatagaaat agttggagga
      961 aatatatgct ctcatggtta gcacgcgtta ttaaagggat tgtaattgct cttggattta
     1021 tcctaccggg aatttccgga ggggttctag cagcaatctt aggaatctat gaacgaatga
     1081 ttggctttct ggcccatccc tttaaagact ttaaagaaaa tgttttgtac tttattccag
     1141 ttgccatcgg tatgcttctg ggaatcggct tattttccta cccgattgaa tacctgcttg
     1201 aaaattatca ggtttttgta ttatggagct ttgcgggagc tattatcggt acagttccta
     1261 gcctcctcaa agaatcaact cgagaatctg accgagacaa gattgattta gcttggttat
     1321 ggacaacctt tatcatttct ggattaggac tctatgcctt aaattttgtc gttggaacct
     1381 taagcgccag ctttcttaac ttcgtcctag caggcgcact attggccctt ggcgtcttgg
     1441 ttcctggcct cagcccatca aatttacttt tgattttggg actctatgct cctatgttga
     1501 ctggttttaa aacttttgat ttcttgggaa ccttctttcc gattggaatt ggtgcaggtg
     1561 caactctcat cgttttttca aaattgatgg attatgcctt aaacaactac cactcacgtg
     1621 tctatcattt catcatcggt atcgtcctat caagtaccct tttgatctta attccaaatg
     1681 caggaaacgc tgaaagtatc caatacacag gactttcact tgtcggttat gtcatcatcg
     1741 ccttcttctt tgcgctggga atctggcttg gtatttggat gagtcaattg gaggataaat
     1801 ataaataatg gcaaaaaaag ttaaaatcaa aaaaacattg gtggaacaaa tcctatctaa
     1861 agcagctatc cctcatcagg ggattcaaat caatgcccta gaaggagagc ttcctcaagg
     1921 ttatgaacga gatcagattt tcaaaacctt ggcgcttttg ggagacaaga ccggaccgat
     1981 tatcggaatt gtccctatca ctcagcactt gtctgaaaag aaactagcta aaatttctgg
     2041 caataaaaaa gtgagcatga ttccacaaaa ggacttagaa aaaacaactg gttacattca
     2101 tggagccaat aatcctgtcg gaattcgtca gaaacacaat tatcccattt ttatcgataa
     2161 aattgctcta gatttggatc aaatgattgt ctctgcaggc gaagtcggac acagcattat
     2221 cgtcgcgcca caagacttgg ctagctttgt aaaggcagac tttgtagata ttttggagga
     2281 catcaagtaa tgaaactcta ctttgtccgc cacggtcgta ccctctggaa ccaagaaggt
     2341 cgttttcaag gtgctagtgg tgattctcct cttcttcctg aatccattga aaccctaaaa
     2401 cgacttggtc agtatctcaa ggaaattcct tttgatcaga tttattcaag tgatttacct
     2461 cgagcggtca aatctgctga gattatccaa agtcaactct ataccccctg ttctttagaa
     2521 atcgttccta atctccgtga atggcaactt gggaagttag aaggtttgaa aattgcaacc
     2581 ttggaagcta tttacccgca acaaatccag gcttttcgtt caaatcttgc tcaatttgac
     2641 actcgaatgt tcggagccga atccctctat agtacaactc aacggaccat ccaatttatc
     2701 aaatcattaa aagatagtcc agctgaacgt attctaattg tcggacacgg cgccaatctt
     2761 actgccagtc ttcgtactct cctaggctat aaagaaccac ttcttcgtaa agatggcggt
     2821 ctagcaaatg ccagcctgac cattatagaa acccatgatt ttgaaacatt cactctcaat
     2881 acttggaatg atacttctta tcaatcataa ttatagccta gccttctgaa ctagaaccat
     2941 aggagatata gtcagttgaa acaaaaataa gataaaagag cctcgtaaaa aggtattgca
     3001 acttgccaat accttttttg atatgaacac gtgtcttctc aacaggattg tagagcaaaa
     3061 tgaaataaga actgaataat atgatttgag aatttaaatc aatttataag aatgttttag
     3121 aagcagttat gtactatttt agattcactt ttctatacta agatgagtag ggaggaaaag
     3181 gtaaaagttt atgcccaaac tcttcacata agaactctag cttacccatt ctatggaatc
     3241 ttgcattatc cattaaaact tccagactca tacccttcta aaatctattc aaaccacgtc
     3301 agcttcacat tatatatgtt ggttacctca caagttttat ctgcaacctc aaagctgttc
     3361 tttgagcagc ctacagctag cttcctagtt taatctttgc ttttcactga gtatcaagtt
     3421 aaaaaatgag gctggtcaaa aagtctttaa aataaaaaaa acgcatatta tcaggtgtta
     3481 aatgaactgc accccaaaag ttagacagaa aaaatctaac ttttggagtg tttttattat
     3541 gaaattaact tatgatgata aagttcagat ctatgaactt agaaaacaag gatatagctt
     3601 agagaagctt tcaaataaat tt
//
LOCUS       DAVLFB010000137         3606 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLFB010000137 DAVLFB010000000
VERSION     DAVLFB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.137"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(183..794)
                     /gene="rpsD"
                     /locus_tag="VXD58_001991"
     CDS             complement(183..794)
                     /gene="rpsD"
                     /locus_tag="VXD58_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV0676625.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(989..2041)
                     /locus_tag="VXD58_001992"
     CDS             complement(989..2041)
                     /locus_tag="VXD58_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV0676626.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2038..2736)
                     /locus_tag="VXD58_001993"
     CDS             complement(2038..2736)
                     /locus_tag="VXD58_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV0676627.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGCETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2903..>3606)
                     /locus_tag="VXD58_001994"
     CDS             complement(2903..>3606)
                     /locus_tag="VXD58_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV0676628.1"
                     /translation="EKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDN
                     GEAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVY
                     GNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDS
                     MPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALA
                     GLLGGLGLKNKKEEN"
BASE COUNT          984 a          810 c          704 g         1108 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcacttta ctatatcact ttggactata
       61 tcggtctagt tatgtctaaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
      121 agggagaacg tcccaaagtg cttaatatca aggcctcaaa gcctatctta tttaataaaa
      181 tattacaaca ttttgttgta gaattcaacg acaagtgctt cgttgatttc tgggttgatt
      241 tcgtcgcgtt ctggcaagcg agtcaatgaa ccttccaact tttcagcgtc gaatgatacg
      301 aatgctggac gtccaagagt agcttctact gcttcaagga ttgctggaac tttcaatgat
      361 ttttcacgaa ctgagatcac ttgacctgga gttacgcggt atgatgggat atcaacgcgt
      421 ttcccgtcaa caaggatgtg accgtggttt acgaattgac gagcttgacg acgagtagtc
      481 gcgagaccaa gacggtaaac aacattatcc aaacgacgtt ccaaaagaag cataaagttg
      541 aaacctagga ttccaccttt gatttttgta gcttgtacga acaagttacg gaattgtttt
      601 tcacctacac cgtaagtgaa acgaagtttt tgtttttcag ccaattgcaa accatattct
      661 gacaatttag aacggttgtt tggtccgtgt tgtcctggta catagttacg acgtgccaat
      721 tctttacctg tacctgtaag tgaaaggcca aggcgacgag cttgtttcca agatggtcct
      781 gtataacgtg acatatgaat gtcctcctga tataaataat atttcggtgg aaacagtcac
      841 ttagaaagcc ctgattcgtg cagatgccct tcgcctaaac agccaaggtt acttgtcata
      901 agacacctgt tgacgagctt catgctttcc tgctgatatt tcacactaaa ttcattttac
      961 catgaatagc tggatttgta aaggggtttt aggctttatt ttcactacca gagaggttga
     1021 gaacgagggt aaaggtactt cctagaccgt actggctgct gactgtgatt tccccaccca
     1081 attgatgggc caattcacgc gcaatcgcaa gtcctaatcc atgaccacct gtcttcatgt
     1141 tacgcgaagt ttcgacacga taaaggcgtt tgaaaatatt ttccaaatcc tctggggcaa
     1201 taccctgccc ttcatcggtc acactgattg aaagctggtc cttctccagc ttagccacca
     1261 cttccagctt ggttcctgga gcagaatatt taaaagcgtt atcgaccaga ttcaccaaga
     1321 tacgagaaag cttagcataa tctccctcaa tccgggcaga ctctgggatt acctgcaagt
     1381 ggacatctct tctctcctgc tcaatcaaaa actgaaattc actcatgcac tcaattaaga
     1441 gcttgtccag aaaaatactg tctttactgg tagtttccac ctgatttcta gctgtgttta
     1501 gggtcaaaaa attcaactcc tcaaccagtt tattgagcct ctccgtctgg cgtccaatgg
     1561 ttgctagata atgagcttgc tccgactcct tgataatacc atccaaaatc ccttctaccg
     1621 tcgcttggat cgaagtgata ggagtcttaa tatcatgcga caactgggca atcatcaagc
     1681 ccttttctcg ttcgctttct tccaaggaat caaagcttac ctgcaaatca tgggacatct
     1741 cattaaaagt ttgccctaat tgctgaaatt ctacaggacc ttgaacctcc aaatttgaag
     1801 gaaaatcctt ggccgctacc cgcttggcat gctccttgag tttgcccaac gacgtaaaga
     1861 ctggcaatag gagaaagaga ctaatcccag caccgacaag gctggcaacg atggtcatcc
     1921 caagcaaaaa gtaaatctcg cctttcgcaa tcagcatttt ttgaacagcc caaaaaacaa
     1981 ccaaaatggt taagagggtt gaaataatat atccaaccaa aatataactt tttagtttca
     2041 tgtttgtcct ctcggtttct ctatcttata tcccaacccc caaactgtct taatagtggg
     2101 cgtttggtca ctactatatt ttgccagctc ctgtcgaaga gcatggatat gcacattcaa
     2161 ggtattggtg tcatccacgt agtcttcttt ccagatcttt tcatagaggt ctgtcttgga
     2221 gaaaactcgc tctggattgc tagctaaaat ccacagcaat tcaaatgatt taacagttaa
     2281 atccagcatt tcttctccta tttgaacttc atgactacta tgattcattt ttagattgcc
     2341 aagggaaatc agctctgttt cgcacccacg atgaaggcgg cgcaaaatat tgtggacacg
     2401 caaaactagc tcacgtgggc taaaaggctt agcaataaaa tcatctgctc ccaagctcag
     2461 gccgtaaatc ttgtcctgtt cactggtctt agcagtaata aataggaaag gctgctctgg
     2521 tgataagtat tgaacctcac tgattaaatc ataaccatcc atccgaggca tcatgacatc
     2581 tgtgataatc aaatcaatcg gttttttctt gaacagctct agcgcttcca gtccatcatg
     2641 ggctaccaag acctgataac ctgcctgaat taagtatctc tgatgaatat ctgtgatttc
     2701 tacctcgtcg tcaacgagta aaattgtctt tcccatctgt ctctcctttg ataaaaacag
     2761 tgctatactg ctttaagtat aacactattt ttattgtttt acgatgattt taatctgcta
     2821 ttgaaagaaa gtcctgattg ctgattgagt tgttaaacta atcttagtca gcccactatc
     2881 catcatttat ttccttagct gattagtttt cttctttttt gtttttcaaa cctagaccac
     2941 cgagtaaacc tgcaagcgca agcccaagga aaccaatact tgccattgat gtttgagtct
     3001 caccagtatt tggtagcata gctttatcct ctgacatcat cgtatcagac atcttgttag
     3061 cagaagcagc catgttttca cctgccatcg tgttggtaga acttgtcatg gtgtcagcag
     3121 gcatgctatc tgtagtacct gtagcatgat tgtgattcat cggagtcacg ccagaaccag
     3181 agttagaagg tgataatgaa ccatttgctg tgtctgatgt ttctttaaca tttatcttaa
     3241 tagtgacttt tttagttgct acgatgttgt ccaagtctgg tttaccgtct ttgttaccat
     3301 agacattgac tgtagcgctg taagtttgag taccgtttgc tcggaactgg tcaatgagcg
     3361 cttgtttttc tttgccagct acatttccgt ccaaggctac ttgatagaag tattgacctt
     3421 tggtcttcac gttttcgcct agtggagata gggctgggtt tttagcctca ccgttatctg
     3481 accatggtgc cttgtcagat gccttgagca agagacgagt caacatgcca tcacctgcga
     3541 agagttcgta tggaatcaca tggttgacac ctgctgtgaa tggaccttca cccttggctt
     3601 tttcta
//
LOCUS       DAVLFB010000138         3594 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLFB010000138 DAVLFB010000000
VERSION     DAVLFB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.138"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..232)
                     /locus_tag="VXD58_001995"
     CDS             complement(<1..232)
                     /locus_tag="VXD58_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HEV0676629.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKI"
     gene            complement(532..3090)
                     /locus_tag="VXD58_001996"
     CDS             complement(532..3090)
                     /locus_tag="VXD58_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV0676630.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
BASE COUNT         1163 a          773 c          532 g         1126 t
ORIGIN      
        1 ttattttttc ggtgatgtac accaactcca aaggaacgtt aataaacatc tctgtaaaac
       61 gggtcatatt atctctattt ttgagaaaaa aacttttctc aatatcaatt ggatttactt
      121 tatctccagt ttttcttcca aaagctattc cccttccaaa aagaagaata tcgatatctt
      181 ttttactttg cgcaattacg gcattgtggt ttagtattcg ttttatgatc ataaaatttc
      241 ccttttaaaa taaaaggaac ctacttaaaa acatcaagaa acttcttctg atgtgattaa
      301 ataggttcct gctcacttgt attagtgata acatcctttt atttcaattt ttaatggtga
      361 catgaagctt aaataattaa taactctgta accgttttta ttttatgata tttcaatcta
      421 tttgtcaagt acttataaaa atttttttat tttgtgtgat tcgaatgtga taatggcaac
      481 agtctcacta aagaaatgaa aatcatacat agaaattttt aaaaattttc tctatcgtaa
      541 acctgccact agttcagaaa ctatactagc tgattccaaa cgtaatatgg tcatctcatc
      601 taccttccct aataatggca ttgcaccata ccaaacaata gtcttattca ccaaaagaat
      661 aggcgttact atttttgatt gagtaaaaga cactttaatt ttctggctct tcaactggtc
      721 tagccatgct ttatttgcaa ccgtctctgg tatacatatc tcaatttgac taatcctaaa
      781 ttcttttaga aaattcatca gttttgtctg gtgcacataa ggtaaaataa gcagacattc
      841 ctgtctttcc cctgctaagt cctctctcaa caccttctca tatctactat caacataaac
      901 gaattgtttc tcctcaccct cgatgacacg ataatccatc tttcgataag ctacttgtcg
      961 tttctgaaac atcttttcta aataaggaac atgaatatcc acataatcga aaatacgcac
     1021 caaagactta tccttgtagt ttctatgaat ccgacctgca tactgaatta aattattttt
     1081 ccaagaaaag ggtgctgcca agataagcgt gtccaactga ggtaagtcaa aaccttcgcc
     1141 aatgtatttt ccagtagaca acaaaacaaa ccctttatct aactgttcta acgtctccag
     1201 taaactcgtt ctttctcgga ctttggtttt tccgctaata atgtaacagc catcaacctc
     1261 tttctctttc aataactttt caaagacatc tatctgttga attcgattaa ctaaaaccaa
     1321 gatattccgt ccttctgcca cttgggctag aatatccttg agaatcaact gattcctcgc
     1381 tgagtctgta gcaatccaat cactgagctg tataaaatta cttgctttgg tcttttcaat
     1441 ctccaaatga ccaaaagaag tgaatcttaa ttgcaattgc cgtttaaaat ccgtttccct
     1501 cttatcagca gtatggagta tctcaccaat tctctgaaaa acaataggct catgaccatt
     1561 cttacgctca ggcgtagccg tcaaaccgta aagatacttc cctctaaact gagcaacaac
     1621 tttttcaaac atcaaggcag agacatgatg acactcatcc acaatcatca tctcatactc
     1681 atccaaaaga ctttgactat tttccaactt aaatagagat tgaatcataa cgacatcaac
     1741 cagtttactc agccatttct tagtcccacc gtactgtcca acatagccga ttaccttttc
     1801 acgacctgat gccgtataac ggatagcctc ctcctcttcg aaagtcaaaa agcagtttaa
     1861 gcgatctagc cattggtcta agagttgctt attatggact agaataattg tttttgtttt
     1921 ccgttcagag ataagagcag cacctaaaac ggtcttccca aaaccagtct ccgcatgaag
     1981 taaaccattt tctttagaag tcatatctga cagggctaac tcttgctcaa aagtgagttc
     2041 tcccttaaat tccacgctaa tagacctttg tacctttctc ctatcttcca cagatacctg
     2101 cttaaattta tcttgcaatg gatacagcaa acctcttggc aaccataaat aatgatcgga
     2161 ttctccaaat aaatacattc tctcaggaat ttgataggtt ggctgtcgca tagcctgctt
     2221 taaataaaat tcgggattag aaaatgaagc catatttttc aagaaaaaca gtgtcttggg
     2281 tgtcacagac gatttttcaa gttggatcat attggataag acgatcttca actccttgtc
     2341 caattcttgc ttgcctaact cctcttggat taacagtgcc actttagcag ttgaaatctt
     2401 ctgaatttct tgtagatacc tccattgatc ttcataaggc tgaaactgtt catccacaaa
     2461 gaccgttcgc ccttgatgat aagcttctcc ttgaaaagga agggaaatta aattatcaaa
     2521 tccaccttta ggaagaacat cctgatttgg aaacatgcga tcaaaagaat caaaggacag
     2581 ttgcatactt tcctgcatag ccaattctaa tagtttcttt ccaaacaggc gagcctttcg
     2641 actcagaatc gcttcctcaa agaagaacca aatatggagt ccgtaacccg aacgagaaat
     2701 ctctaaatgg gcttccatct ggcgttccct ggctattctt cgaatggtta aaccagcttc
     2761 tttccaatct ccttcatcca aatccagtac caaaaaataa cagctatcat ctaagtgcat
     2821 aggaaagata ccgatagctg cctccccacg aaaatgagat ttcaaaacgg agttcgtcaa
     2881 tgtctggaaa ctccgttttt caggtggcaa ctgcttccaa ccataatcat aggaaggaaa
     2941 atactgaatt ttcccttgtt cattgataaa attcttagca taaacatcat aacggccagc
     3001 aaacacagag gtaaacaatt gtaatttttc ttgcgtcgtc aagcgactac tttgaagttg
     3061 gaacatctcc tcgaactgag agcgttccat aacaaactga ctcttgtctt taagacgttt
     3121 atagtggaat gaaataagat atgaacaaat tgattaggaa aatcaaatta atttatagaa
     3181 atcttttagc agtcatggcg tcctattcca gattcaaaat gctatacaca agaaaaactc
     3241 actatcccca ttaaaagaag aaaagacatc aaggacttct accacaattc catccaaaga
     3301 cacaaaaaca gccataagag tcacctcctt gattcctata ggctgattat aacaagactg
     3361 gctgaaattg tacatgaaaa taaaatccta atagtactca ttttgtatgt gactaatatt
     3421 ccgtctcgct ccagaaggta cgaagtaaat agagtttaaa cgagcagtct ataaaattca
     3481 aaaaacgcat attatcaagc gacctaaaac tttgggctct ataatatttg tagtgggtaa
     3541 attccctatg gatattatgg agcctatttt gggtagaaaa aaagtcccat aaga
//
LOCUS       DAVLFB010000139         3593 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLFB010000139 DAVLFB010000000
VERSION     DAVLFB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.139"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(124..1260)
                     /gene="lctO"
                     /locus_tag="VXD58_001997"
     CDS             complement(124..1260)
                     /gene="lctO"
                     /locus_tag="VXD58_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV0676631.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1885..3375)
                     /gene="lysS"
                     /locus_tag="VXD58_001998"
     CDS             complement(1885..3375)
                     /gene="lysS"
                     /locus_tag="VXD58_001998"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV0676632.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTVNSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3503..>3593)
                     /locus_tag="VXD58_001999"
     CDS             complement(3503..>3593)
                     /locus_tag="VXD58_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV0676633.1"
                     /translation="LEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1023 a          774 c          706 g         1090 t
ORIGIN      
        1 cgatttaact gtcctgattg atttgtcctg tttttattcc attttactat ggttttcgat
       61 agcgatttat tcttccaata cacgaagaaa aacctccaca ttcagtggag gcaatctctt
      121 ttatcaatac aattttaagt cacgagggtc aactgggaaa gttgggttgt atgggttgtg
      181 acggagtttg aagtgtttaa catcttcaat agtctgagtt ccagacaact gcataactgt
      241 cttcaattcc gcattcaagt gttcaaagac ttgacgcaca ccaacactac cgccgagagc
      301 caagccatag ataaccgggc gaccaatagc taccaagtct gctcctgaag ccaaggcttt
      361 aaagacatgt tgaccacgac gaacaccaga gtcaaagaca attggcacac gtttatcaac
      421 tgcttctgcc acttcttgaa gcgagtcaaa ggcagctggt ccaccgtcga tttgacgacc
      481 accgtggttg gttacccaga taccagaagc tcctgcagca agcgaacgtt caacgtcctc
      541 acggcattgt ggtcccttga catacacagg aagtcctgag tattcagcga taaattctac
      601 atcacgtgga gacaagcgtt gtttagctga tttgtaaaca aagtccattg atttaccagc
      661 accttctggc aggtattctt caacaatcgg catgccaact gggaagacaa aaccattacg
      721 cttatccact tcacgattcc cccctacagt agcatctgcc gtcaagacaa tcgctttata
      781 accttcagcc ttcacacgat ccatgatgtg gcggttgata ccgtcatcct tactaaagta
      841 aaattggaac caatggggtg tcccttgaag ggcttcagaa atctctggaa ggtcaacagt
      901 agagtaagaa ctggttgtat aaagagaacc aaactcatgc acaccacgcg cagtcgccac
      961 ttccccctgt tcattcgcta atttatgggc cgcaacaggc gccataatga ttggtgaaga
     1021 tagtttttca cctgcaaatt caatctctgt acttggattt tcaacatcgc aaagtgtatg
     1081 aggaacgatg agtttgtggt taaaggcgcg gatattctct cttaaagtga aagtatcttc
     1141 cgccccacta gcgatatagc caaatgctgc tttaggaata acttgttgcg ccattggctc
     1201 caaatcatag gtattgatga agtctacatg accttctgca ttgcttgttt tgtatgacat
     1261 aaaatgccct ccttgattaa gtaagcgttt gctttttact acacaactat aataactctt
     1321 ttttcaatca ttttcaagag ttttgcttaa aaaccatctt attttttcat acaatatact
     1381 agccctactt tccttaaact ctaaacctta gtctatatta agtgaatcgt tgagattttt
     1441 tgctgccagg catttgaact acttttttat ttgcggattc tatctactaa tactataaaa
     1501 aaacaagtga ataagacacc agaacatcct ctaataggac ctatggactt atatcagtcc
     1561 catatatact gtttaagcaa gatactatcc tagttgacgc taacttccta tcccacttat
     1621 taatttttta tttatagtga atctgaacaa atcaattggg gaatccaagt caatttctag
     1681 tactttttga acagtttcag cgtactatcg taaaatgaaa taaaacatgc acaaattgct
     1741 tagggaattt aaaccaattt ctaacaatgt cttagaaatc aaagtgtact atttcaactt
     1801 caatatacta cacttcattt tctccttaaa ttcagtctct ttttgaatca aaaaatcccc
     1861 tggtaagagc cagaggataa gaatttattt cattgttggg aagagcaata catcacggat
     1921 agttgttgta tcagtgagga gcatgcagag acggtcgata ccgattccca aaccacctgt
     1981 tggtggcata ccgtattcaa gagcttcaat gtagtcatag tcgattcctg tcgcttcatc
     2041 atcaccaagt tctttggctt tagcttgggc ttcaaaacgg ctaagttggt cgattgggtc
     2101 gttcaactca gtaaaggcat taccgtactc cttagtcatg ataaagagct cgaaacggtc
     2161 agtaaagcgt tggtcttcag gatttttctt agcgagtgga gatacagcta ctggatgtcc
     2221 atagacaaag gttggttgga ttaaagtttc ttcaacaaac tcttcaaaga aggcattgat
     2281 gatgtgacca acctcagtgt agtgtttctc aactggaact ttcttctcag cagcgatagc
     2341 tttagcttct tccaaagtca tgtcttgcca gaaatcgaca ccagtaattt ctctgatagc
     2401 atccaccata tgaacacgct taaatggttc gttaatcttg atttcagtac cttggtagtt
     2461 gactgggcca tcacctttga ctgatttagc agcgtgttgg ataatgcctt cagtcaagtc
     2521 catgatgtct tggaagtctg cataagcttg gtaaacttcg atagaagtga actcagggtt
     2581 atgagtagcg tccattcctt cgttacggaa gatacggcca atttcataga cacgttccat
     2641 accacccacg ataaggcgtt ttaagtgaag ctcagtcgcg atacgaagca ccatgtcaat
     2701 gttttgggca ttgtggtggg tgataaatgg acgggcagca gcaccaccgg cttcattatg
     2761 aagaacaggt gtttccactt caaggaatcc tttttggtca aggtaacgac ggatttcaga
     2821 gatgattttt gaacgagtga caaagcgttc aaagctttca cgattagaaa tcaagtcaag
     2881 gtaacgttta cggtaaattg tttcaacgtc tgtcaaacca tggaatttct caggaagagg
     2941 acgaagagcc ttagacaagt gtgtgatgtg ggttgccttg atagagagtt ctcccatatc
     3001 cgtacgcatc acttcacctt cgacaccaag gaagtcacca aggtctgctt ttttgaagat
     3061 ttcgtagttt tcttcaccga cagcatcctt acgaacgtag atctgaatct ggccttcgcg
     3121 gtcttgaagg tgggcaaaac caacttttcc tttaccacgt ttggttacca agcgtcctgc
     3181 gatagtagct gtttcgtttt tatcgtgtaa ttgttcttta tcgaggttgg tatatttatc
     3241 ttttaattct tgtgaattta cagtacgttc aaaacgtttt ccgaaaggat cgattccttg
     3301 ttcgcggagc gcagccattt tttcacggcg aacgatctgc tggtcattta gttcttccat
     3361 atgttctgta gacatgggat cctcctagtt ttactcaaaa ttgaatacga tgagtcattt
     3421 tttgcgatac tcccatttta tcataaataa gcaagaaatt cacggataat ctttaaaaac
     3481 taaaaagaac ctgacgctaa aatcactgga aagaagtgaa agtatctccc gccccactag
     3541 cgatatagcc aaatgctgct ttaggaataa cttgttgcgc cattggctcc aaa
//
LOCUS       DAVLFB010000140         3500 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLFB010000140 DAVLFB010000000
VERSION     DAVLFB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.140"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..107
                     /locus_tag="VXD58_002000"
     CDS             <1..107
                     /locus_tag="VXD58_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV0676634.1"
                     /translation="ALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(141..1475)
                     /gene="ciaH"
                     /locus_tag="VXD58_002001"
     CDS             complement(141..1475)
                     /gene="ciaH"
                     /locus_tag="VXD58_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV0676635.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(1465..2139)
                     /gene="ciaR"
                     /locus_tag="VXD58_002002"
     CDS             complement(1465..2139)
                     /gene="ciaR"
                     /locus_tag="VXD58_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV0676636.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            2357..>3500
                     /locus_tag="VXD58_002003"
     CDS             2357..>3500
                     /locus_tag="VXD58_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV0676637.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYYD
                     LAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGL
                     IEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIK"
BASE COUNT         1115 a          751 c          561 g         1073 t
ORIGIN      
        1 tagcattaaa attatcaact gctcttgcct ttgccgcagc aatcggattc agcttacttg
       61 ttttaaaagt tcaacggtct attatcaaac gaaaacgtag aagataaatc caaaatatcg
      121 ccccaattgg agcgatattt ttattttttc tttttagatg gtgtctgaat ggcaatcttc
      181 acttcaaaga ttgttccctt gggtttatta tctttgacag taacagttcc ttttagagca
      241 tctacaattt gcttggctag ggataatcct aaaccaaaac cacctttttg ccgggttcta
      301 gctttgtcta ctcgataaaa acggtcaaaa attttctttt tatcttctgt cgaaataccg
      361 attccattat cagaaacaag taaataaaga ttgcgatcgg tcgccgagat aagaaaatca
      421 atttcaccat cctcctcagt atacttgacg gcattatcga aaagaatggt catcagttgt
      481 ttcagaagaa gctgatctgt gacaattgtt cgatggatac gattttcaaa acggaagaca
      541 cgattatttt ccgaagcaat catctcatag tttgtgaaag ttgtattaaa aaagctagtt
      601 ggaacttctg caagctccgg cttaatccca tcatctctcc gagctaagtt cagcaagctt
      661 gtcgttaaaa aacgcatatt tcggacttct tccaaactcg atgcaatgct ttcgctcaca
      721 tccataatgg tagcttctgg cttacgaaaa agggtctcta agcgattttg caaaactgcg
      781 agtggagttc gtaactcatg actggcattt tccacaaaag actgttgctt ctgcatactc
      841 tcaagcaggg gcctaacact gaccctagct agatagagac tggcaagtaa agacaaaatc
      901 cagaaactag ccatcacgac cacaatcaat tgctcatgct tttgactagc ctgctccaac
      961 tgactggtat taatcaagac agcagcatac ttgatattgg ttgaaaccga actaatattg
     1021 gtctccatca aaatcacacg atagatttct tcctgcccat agctattaaa aacctgaatc
     1081 tggtagatat gtcctagttc tttcttttct aacttaatct tatccaagcc caaaaatcga
     1141 tttccagaaa gaagttgagt aaagtcttta tcaaagagaa tgacttccgt attggaactg
     1201 acattaggtt ttatttctac tttactagcg tccgccctag cattttctaa atctttaatc
     1261 tcttctgttg ccctatttat agccagctga ataactgctt gaggattttc actcagtcca
     1321 tgaagcttat cgtccaccga agtataaaga ctcgaatgca tgacttgtaa aataatcaga
     1381 gtcattgtag aaaaaatcag ggtgaagaca ccgaagttgc ggataaaata actaaagtca
     1441 tccgcatacc atgttttttt aagtttactg aacatctttt aaaagatacc caacactacg
     1501 caaggtttgc aaattctctg caaaagtggt tccctttaat ttcttacgga cttttgaaac
     1561 atagacttcg acaaccgaaa tcgttgtatc actatcaaat ccccatagac ggtcaaaaat
     1621 ctgcgtctta ggcaaaatca cattttgatt ttgaaggaaa taaactagta aatcgaactc
     1681 tttccccagc aattcgacag gagtatcttc aactttaacg gtattggttg ataaattaac
     1741 cacgatattc ccataagtca aggtgttttc attaaacttc cctgaacgtt tgagaagggc
     1801 ctgaattcgc attttaagtt cttctaggta gaaaggtttg gtcagataat catccgctcc
     1861 cagttcaaat ccatgtccct tgtcatccaa actttccttg gcagtcataa tcagaactgg
     1921 tgtcgtaatt cccttttcac gcaattcttt taagacttgg aaaccatttt tttctggcaa
     1981 catcaaatcc agcaaaatca agtcatagac accactctca gcttcgtaga gaccttcttc
     2041 tccatcaaat acctgcataa catccgcaaa atcgtctaaa aagtcaaata ctgaatttga
     2101 cagacctagg tcatcctcaa ccaataagat ttttatcatg agaaactcct ccttattaaa
     2161 actattatac caaatttgcc ttaaaaaaaa ctcaactctc tgcattttac atgagatagc
     2221 tgagttttct ttggctctat aatatttgta gtgggtaaat cccctatgga tattatggag
     2281 cttatttttg tgtagaaaaa aagtcccata tgacctataa tgaaaagcga caaaacaact
     2341 cattagaaag aatcatatgg aacaattaca ttttatcaca aaactgctcg atattaaaga
     2401 cccaaacatc aagattctag atatcatcaa tatggatacc cacaaagaaa ttatcgctaa
     2461 gctggattat gaggctccat cttgccctga ttgtggaagt ctaatgaaga aatatgactt
     2521 tcaaaaaccg tctaagatcc cttacctcga aacaactggt atgccttcta gaattctcct
     2581 tagaaaacgc cgtttcaagt gctatcactg ttcaaaaatg atggttgctg agactcccct
     2641 ggtaaagaaa aatcaccaaa tccctcgtat catcaaccaa aaaattgctc aaaagttaat
     2701 tgaaaaaatt tctatgactg atattgccca tcagctggcc atttcaactt caactgtcat
     2761 tcgcaagctc aatgactttc actttgagca tgatttttct cggcttccaa agattatgtc
     2821 ttgggatgag tatgccttca ctaagggaaa gatgagtttc attgcgcaag attttgataa
     2881 tcttaatatt atcactgttc ttgaaggcag aacacaagct gtcatccgaa atcactttct
     2941 tcgctacgat agagccgttc gttgtcaagt gaaaatcatt acgatggata tgtttagtcc
     3001 ttactatgac ttggctaaac agctttttcc gtgtgctaaa atcgttctag atcgtttcca
     3061 tattatccaa catctcagcc gtgccatgag tcgttttcgt gttcaaatta tgaatcagtt
     3121 tgaacgaaaa tctcatgaat acaaggctat caagcgttac tggaaactca tccaacagga
     3181 tagtcgtaaa ctcagcgata aacgttttta tcgccctact tttcgcatgc acttaacaaa
     3241 taaagaaatt cttgacaaga ttttaagcta ttcagaagac ttgaaacacc actatcagat
     3301 ctatcaactc ttactttttc actttcagaa caaagaccct gagaaatttt tcggactcat
     3361 tgaggacaat ctgaagcagg ttcatcctat ttttcagact gtctttaaaa cctttctaaa
     3421 gaacaaagag aaaatcgtca acgcccttca actaccctat tcaaacgcca aattggaagc
     3481 gaccaataat ctcatcaaac
//
LOCUS       DAVLFB010000141         3456 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLFB010000141 DAVLFB010000000
VERSION     DAVLFB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.141"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            441..629
                     /locus_tag="VXD58_002004"
     CDS             441..629
                     /locus_tag="VXD58_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676638.1"
                     /translation="MSVDIEAIRWLLDNATAYAISKNCGMSIQAVDKYKNGVSDIMNM
                     RLKHAISMITYAQELKKQ"
     gene            complement(899..1855)
                     /gene="lytA"
                     /locus_tag="VXD58_002005"
     CDS             complement(899..1855)
                     /gene="lytA"
                     /locus_tag="VXD58_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0676639.1"
                     /translation="MDIDTSRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYSKDKFEKINGTWYYFDGSGYMLSD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1859..2191)
                     /locus_tag="VXD58_002006"
     CDS             complement(1859..2191)
                     /locus_tag="VXD58_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV0676640.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKEYLVKKGGEKTIKIVE
                     ILAKNAVHAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(2195..2611)
                     /locus_tag="VXD58_002007"
     CDS             complement(2195..2611)
                     /locus_tag="VXD58_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV0676641.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(2621..2971)
                     /locus_tag="VXD58_002008"
     CDS             complement(2621..2971)
                     /locus_tag="VXD58_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676642.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(2974..3177)
                     /locus_tag="VXD58_002009"
     CDS             complement(2974..3177)
                     /locus_tag="VXD58_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676643.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMIASNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(3271..>3456)
                     /locus_tag="VXD58_002010"
     CDS             complement(3271..>3456)
                     /locus_tag="VXD58_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV0676644.1"
                     /translation="KHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIK
                     AIQELNQKIEKMEKTIA"
BASE COUNT          932 a          742 c          656 g         1126 t
ORIGIN      
        1 tcaacactct tggagggtgt cccctccaac tccccgacct ctggacaagg tctatttttt
       61 tgaaaaaaat ttattaaaac cattgacttt ctacaactaa agttgtataa tagatacata
      121 aggttaagga ggaaacctta gacaaggaaa ctagtagaaa ggaacatgaa atgtttaagt
      181 tcaaaaagaa accactcaaa gtcaaaacaa ataagctagt catcaaaata aacttattta
      241 taatcagctt tgaatggcac ttagaaattg gatagtgaga aatcactatc caccccttag
      301 gggggtgtac ttaaattata acaggaaaaa caatgaaagt aaatcttaaa attagaaaaa
      361 ccaccaagcg tgaaaaagtt gaattcatta ttggatttgt tctacttcta tttgcgattt
      421 ggtattttac gaggtaatat atgtcagtag atattgaagc tatccgctgg cttttagaca
      481 atgccacagc ctatgctatt agcaaaaact gtggcatgtc tattcaggcc gtggacaagt
      541 ataaaaatgg tgtgtcagat attatgaata tgcgtctaaa acacgctatc agcatgataa
      601 catacgccca ggaacttaaa aagcagtgat ttcgatcact gtttttattt ttaacaaaac
      661 acctccctat ttttcatgta aacgtttttt tgaacaatag gatttagatt tctgtttcta
      721 atcgttcaaa atgcgtgttt ttgaaaaata gaacttaact ctaatttttt aggcaaacca
      781 tttattttgc taccgacatt ggtgtcggtt ggttagacgt tttcttgatt cagacaaaca
      841 aaaaaaccgc aagcctgagc ctgcggtgaa agaacaattt agaaagtttc ctttctattt
      901 atttaactgt aatcaagcca tctggctcta ctgtgaattc tggcttgtct gccagtgttc
      961 cgtctggttt gaggtagtac cagcctgttc cgtctgcgga ctggacgaag gcgtttgata
     1021 ccatagcgcc ttctttagcg tctaagtagt accaagtgtc cttgtacttg acccagcctg
     1081 tcttcatggc accttcttcg ttgaaatagt accacttctc agcaattttc ttccagcctg
     1141 tagccatttc gcctgagttg tcaaaccagt accagttgcc gtctgtgtac ttcttccagc
     1201 ggtctgaaag catatagcct gagccatcga aataatacca ggtaccgttg attttctcaa
     1261 acttatcttt tgaataagag ccgtctgagt gtacgtacca gtagccagtg ccatttttct
     1321 gccagcctgt tgcagcgctc aagccgtttt cgatgtcttg cttaaactgt tcacggctaa
     1381 tgccccaact tgcaagatat ggatatggat ccacatggtc tgagtggttg tttggttggt
     1441 tattggtaca gtattcatgc gtcttgatac ctgccaagtc gtctgtatca agagtcttcg
     1501 gcaagcctgc ttcgtccgct agattgcgta gcaattcgat atagaggcga tagtcagcca
     1561 taaactcttc cttagttgaa tggctttcaa tcagttcaac cgctgcatag gtctcagcat
     1621 tccaaccgcc cccaacatcc caacttccgt tgttcacagg tcctacctgc atgacacggc
     1681 cgttcccaac aacgtgcgag aaaaaaccta attctgggtc tttccgccag tgataatccg
     1741 cttcattctg tacggttgag ttgcgattac ctgttgagtg agcatgtact tgtcgataag
     1801 gctgcacccc aacctggggc aagcctgtac gtagtctgct tgtatcgata tccattccct
     1861 atcgtccttt ccatgcgtcg ttcatctgtt tcactgcgga ctctacgaag gtgtctaagt
     1921 ctttgtcagt catgctaata ttgtattttg taagctcagc acggacttta gcgcgagctt
     1981 gtgccagttt ttcatctccc ttgtagccag tttcagcagc tacttgctca acggcatgaa
     2041 ctgcgttctt ggccaagatt tcaacgattt tgatggtctt ttctccacct tttttgacga
     2101 ggtattcctt gactgcccta actaccaccc ctgccaaaac gactaggatg ctgattgctc
     2161 cattagtaat gatttcagta atttgttgca tttgttattc tccttttttc gtatcatcat
     2221 ctttttcaag taatcgctga aatactttta caatcggctg aaaaagagta acatttcctt
     2281 ttaatttgcg gtaattttca atgagagatt gaaaagtaaa tgcgatgtac ccgagataga
     2341 ttgagtgcaa gaatacaaaa cctgtctttt caggcaacaa aacggacgcc ggaataagga
     2401 tcatcagtaa gagaacccct gaaatcttac gaaggagccc gttaatgccg attttgctct
     2461 tgtactcgat gtcaggattg ataattgccg caatcgtccc tgtcacaaaa tcaatgattt
     2521 ccattgagac aatcagtgct agagcgtaca agaccaaacc atcttcagtc tgtacgacac
     2581 ttcttagaaa attgaaaaat tcaatttgca tacacacctc ctattcttta ggttttaccg
     2641 ttggatccgt ccagtcagga ttgccctctg catcaaattt catgatatag aattcatgat
     2701 tcaacagaac ggctacgttg attgttgcga ttgtaccacc ccactggttg aacgcccaaa
     2761 cggtttcaac atccttgaat tggcgacggc catttacgat cacaggacgt ttttgaacat
     2821 cacgatacat atagaagtca tcgcttacat tcttgcaacg aatgaactct ccattttctt
     2881 tcatgtagcg caaagcactc gcaagatcaa atggttctgt gatttttgta aggtctagta
     2941 agttatctgt gttttgaatt gtttctgcca tgtctattct cctttgtctg ctggtttagt
     3001 ttgttcatca agcagagctt ccagctcatc cactcgtgct tgaagtcttt gattctcttc
     3061 cctttgctca tttaactgaa tactcaagaa attacttgca atcatcgaat ctgttgaagt
     3121 tgttgacatt tcacgaattg tcatttgtaa ggcttggtta agctgttctg tgttcatttt
     3181 ctaagttctc caatctgtgt gttcgttttc tattttcaag agcaaactcc tgaattgctt
     3241 taagtgcgat attggtcaac atgttgttat tcatgctatt gttttctcca ttttttctat
     3301 tttttgattt aattcttgaa tagccttgat taagtaagga actaaagcgg tatagtcaat
     3361 atgcagatag ccatctggat tctcaggatc tcgtgagaca attcttggaa cgatggtttc
     3421 agcctcttga gctattagac caatctcctc atgttt
//
LOCUS       DAVLFB010000142         3213 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLFB010000142 DAVLFB010000000
VERSION     DAVLFB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.142"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            72..803
                     /locus_tag="VXD58_002011"
     CDS             72..803
                     /locus_tag="VXD58_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676645.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            818..1969
                     /locus_tag="VXD58_002012"
     CDS             818..1969
                     /locus_tag="VXD58_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV0676646.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(2021..2680)
                     /locus_tag="VXD58_002013"
     CDS             complement(2021..2680)
                     /locus_tag="VXD58_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV0676647.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTCHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            2750..3196
                     /locus_tag="VXD58_002014"
     CDS             2750..3196
                     /locus_tag="VXD58_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676648.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTFQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
BASE COUNT         1175 a          473 c          658 g          907 t
ORIGIN      
        1 gctaattatt gtagtaattt ctttaagaaa atgatataat tcattaaaaa gtatttggag
       61 gaggaagaat tatgttgaat caggatctct ttgattcgct tgaggcacaa aaaattgtag
      121 atactttgat gaaagggcaa aaagattatg tagatgaacg tctagaaaaa agagagacaa
      181 tgatagtgtc gaatggttat gcatggacgc gacctaatca tattgatact gcatttgcat
      241 cagcagattt gtttgagtat aaattacaat tagcaggaca gacttgggga tatttagaat
      301 ttgaaacaaa tacagaaaaa tatgggaaag tattgttaat tataaagggt aagaagcgac
      361 ttacgaacca atttcctttg gtacaaaaaa ataagagtgg ctacttattt gaatatgctc
      421 aaatgaatac actttatctt aatcaacatt cttcctacaa aaatgatgaa gatagtcatt
      481 cctttccaat tcagatggag ttagtttctg atgaaatgat tcaagaaatt gaacaagcta
      541 ccaaaaattc gaatatcgaa aaatttatga ttttaactta tgaggcggac tcagaaaaca
      601 atattatatc tgtagatgtt gttatgcctg atgcacgaac tggtcagtta cacttgattc
      661 aagatttgtc tgagtatatt caatcaagtt cgtaccattt cgaagaagct aaataccaag
      721 atattcctaa tttttcagaa ttatcggaaa cagaagattt tgaaattatt ccaagaatag
      781 aaaaacaaga aggtcaaaag tagtaaggag tatgtaaatg tttaatggtc gggtattgaa
      841 agaattacgg ctgttaaatg gtttaagtag agcagaatta gctcagagaa ttaatttaac
      901 ggaacaagcc atttggcagt ttgagtccaa cgaaacgaaa cctaaattat caaccaaaat
      961 gcatttggcc aaccaatttc atgttgattt aacttatttt gaacaggaag aagagagcat
     1021 tcgatttgat tcttctgtaa ttgcctttag aaatgcagac ctagcaacac ggaaaacaat
     1081 agatattcaa actatgtatt tacataaggt agatagtttg attgattatt ttgaaagttt
     1141 tgtaattata cctaatatta caattcatga cctaagtaat gtagtgagtg aatcttatca
     1201 taagggagaa tccattgagg aattggctct ttatgccagg gaaaaattag gtatttcaaa
     1261 agataatcat gatttgcttt ataaattaga acgttcaggc atctatatcg tggaacgatt
     1321 aattaatggc caagctgatg cttatagcgc atggtcaaaa ttgggaagac cttatattgt
     1381 gttgggaacg aataaatcat ctgtacgtcg aaattttgac ttagctcatg agctaggaca
     1441 tattctttta cataaatata aagatatgaa tgaagatggc gatcgtttgg agcaagaagc
     1501 aaattatttt gcatcatgtt ttttattgcc aaaagaagag tttttagtca aatttgaaga
     1561 gagggttggc aagcgtgtca gcaatcctga tagttatatt ttattgaagt cggatttgaa
     1621 tgtttcgata caggctttag agtatcgagc ttttaagtta ggattattga ctccaaagca
     1681 acattcttac ttttatcgtc aaattgcgca aaaaggttac aaaatgattg aacctttgga
     1741 tgatcaaatt tttgttaaaa aaccaagcaa agtaaagagt attctggacg tcgttttgag
     1801 taatcatcta gtcagtctag cgactataat gtctaaacaa agtattcgtt tacagtttat
     1861 aagcgaaata ttttcagtcg aaatgaaatt ttttgatcag tatcaagaag atagaagaac
     1921 agatcgattt gataacatca tccctttgta caaaagaaat aatttataat ttaactgtga
     1981 aataatttca tgaaggacat atggcagaaa ttggatttta ttagacaagc agtagcttac
     2041 caaaaataat caaacaagta gcaagtaaga ggaaaggcac gaaaggaagt ctttcctttt
     2101 tcttttgcag gagaaaggcc aggatacccg tcgcagaagc gaactgaatc aagatcagta
     2161 actccgttac gctaaagacg agagcacaag aagctaaaaa aaggaaatcc cctgcaccca
     2221 tgcggatatc gataaaatga gccaaaattc caagtatgag gaaggagacc atgaccagat
     2281 tccagccaga ggaagctatt aggattaggt ggcaagtcat ccagaccagt aagggatatt
     2341 cctgatggtg aaagtcgtag atacccaagg tcaaaccagc ggtgattagg acgacttgcc
     2401 ccaaggaaag caatttccaa gagtaaagca gaaagaggag tcctaagact aattcaaaga
     2461 gggcatacca gacaggatag cgaactttgc agtagcgaca gcgaaagcga ttgaagacct
     2521 gtgagagaat cggaatcaaa tctaagggac gcaagcgagt ctgacaggaa tcgcagtgac
     2581 tggctgaact gataatggat tgctctggaa aacggtcaat gaccaaacca agaaaggaag
     2641 cgagaatgct cccgacgaga aaaaaataaa aatcaatcat acttatctat tcgtaaaaaa
     2701 tgggagaaat agtataatgg agaaacatag ataagatggt gaggaaaaga tgacaattcg
     2761 ttttgaagaa aaggtgagta tagaaaacgc tcaatttgta tgccagtggt ccaactccct
     2821 aggcaaatcc tttcaagaac aatggatggg accaaggatt ccttttctac tgacctttca
     2881 agccttggaa ggagtctttt caatctttga tgaacaagag tttgtgggat ttatccagaa
     2941 aatcaggcta gaagacagca atcttcatat cgggagattt tttatcaacc cccagaaaca
     3001 ggagcaaggc ttaggtagcc aggctttaag gaaatttgtt agtttggcct ttgaaaatga
     3061 agatatagat agtatttctc taaatgtctt cgaggcaaat caaagagctc agaatcttta
     3121 ccaaaaagaa ggatttgaaa tcgttcaaat ggttgaagca cctgtacgaa aatatagtat
     3181 attgaaacta gaatagtaca cctctacttc taa
//
LOCUS       DAVLFB010000143         3091 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLFB010000143 DAVLFB010000000
VERSION     DAVLFB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.143"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            142..2931
                     /locus_tag="VXD58_002015"
                     /pseudo
     CDS             142..2931
                     /locus_tag="VXD58_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
BASE COUNT          993 a          613 c          761 g          724 t
ORIGIN      
        1 cttagccgga ctaagaatga actgtctggc gcaagtaccc tacttgcaca ggaagctaag
       61 cggattgagc tggattctgt tgctagactt gaagcgttta agtcgcagac tacgagcgct
      121 cagacggctt tgtcaggtga cttggatgtt ctaaaacgaa ctatcgcaaa cgatattcga
      181 ccgaagcaag cacaggctga agctgagatt gccaagcaag ttgaagtact tagtcggact
      241 aagaatgaac tgtctggcgt gaagtcagcg caagcgacgt atgaggagac gacgactcgt
      301 agactgtcag aactgaccaa cttggccaat ggtaaagcca gcaagtcaga actcacgcaa
      361 acagctgagg agctggctag tcggattgcg agtgtgcagg catccggtcg aaatctattc
      421 ttgaactcac tattcaagca ggatatttca aaaacaggaa tttggacaac gagtacatat
      481 acggctgcta tcgatagcga aagtaagcat cttggacaca aggctcttaa aattataggt
      541 ctgaatccat ctggccgtga tggaggtaat cccaaggtta cttatccagc tctgggtcaa
      601 ttcgggaaag taattcccgg aagtacgact aatcaagatg taaccattag tttttatgct
      661 aaggcaaata aaaatggaat aatgctgaga tctcgattag ggaatatcgg atataaaact
      721 ggaaatgtga cattgtcgac agaaattaag cgatatgttg tccatattcc aaaaggttgg
      781 acaaacgaat ccaagcagac cacaaatgaa tggttgttca atttcaacca ggaaggaacc
      841 gtttggattt ggatgccgaa gttcgaaata agcgatgtag atacttctta ttcagaagct
      901 cctgaagatg tcgaaagcca aatctcaaca gtcgaatcga cctttaaaca gcgtgctgat
      961 tcactcgacg ctggtgtgaa tcgtctgact gaaggcctta gaaccaaagt ggatatcagc
     1021 tcactcaatg tgactgctga gaatatccgg caatctgtga agagtcttga gacagacacg
     1081 cagaacaagc taaatcagaa gttgagtcag gctgaatttg aggtgcgagc cggctctatc
     1141 cgtcaggaaa tcctgaacgc aaccaaggat aaagccagca agtcagaact cacgcagaca
     1201 gctgaggagc tggctagtaa gatagcgagt gtgcaggcat ccggtcgaaa tctattcttg
     1261 aactcactat tcaagcagga tatttcaaaa acaggaattt ggacaacgag tacatatacg
     1321 gctactatcg atagcgaaag taagtatctt ggacataagg ctcttaaaat tataggtctg
     1381 aatccatctg gccgtgatgg aggtaatccc aaggttactt acccagctct gggtcaattc
     1441 gggaaagtaa ttcccggaag tacgactaat caagatgtaa ccattagttt ttatgctaag
     1501 gcaaataaaa atggaataat gctgagatct cgattaggga atatcggata taaaactgga
     1561 aatgtgacat tgtcgacaga aattaaacga tatgttgtcc atattccaaa aggttggaca
     1621 aacgaatcca agcagaccac aaatgaatgg ttgttcaatt tcaaccagga aggaaccgtt
     1681 tggatttgga tgccgaagtt tgaaataagc gatgtagata cttcttattc agaagctcct
     1741 gaagatatag aaggtcagat ttcaacagta gaatcgacct ttaaacaacg agccaactcg
     1801 ctcgaagctg gtgtaagccg tctgactgaa ggccttagaa ccaaagcgga tatcagctca
     1861 ctcaatgtga ctgctgagaa tatccggcaa tctgtgaaga gtcttgagac agacacgcag
     1921 aacaaactaa atcagaagtt gagtcaggct gaatttgagg tgcgagctgg ctctatccgt
     1981 caggaaatcc tgaatgcaac caaagataaa gccagcaagt cagaactcac gcagacagct
     2041 gaggaactgg ctagtaagat agcgagtgtg caggtcgggg gtaggaatta tatccggggt
     2101 acaaagcgca tgatgctagc cagaggattg tgggcatcag gtacttttag accgtcaggc
     2161 gctgggacgg caaagacgat tgatgtatca gacagtccag caactggctt tgataaagcg
     2221 atacgattga cctcaagcaa tgctagagac caaataggta ttgctcaaga tggattttat
     2281 atctcgcaag gcacatacac gatgtcttgt tgggtcaaag gcagaagagg tcaaaaggtc
     2341 aagctacaaa cttattggca agtcaatgat aattcgggta tttcgcccat ctttacatta
     2401 aaggatgaaa attggacaaa gctatcgttt actagcgcta gaaatagggc tggagtcgca
     2461 tcaattggct atgtgtatct cgtaaatgct gaggtcggag aatatttaga tgttcttgcg
     2521 ccccagctgg aagacggaag tttggcaaca agctcaaaag aagctcctga agatatagaa
     2581 ggtcagattt caacagttga atcgaccttc aaacagcgtg ccaactcgct cgaagctggt
     2641 gtgaatcgtc tgactgaagg gcttagaact aaagcggata tcagctcact caatgtgact
     2701 gctgagaata tccggcagtc tgtgaagagc cttgagacag acacgcagaa caagctaaat
     2761 cagaagttga gtcaggctga atttgaggtg cgagccggct ctatccgtca agaaatcctg
     2821 aacgcaacca aggataaagc cagcaagtca gaactcacgc agacagctga ggagctctct
     2881 agtaagatag cgagtgtgca ggcatccggt cgaaatctat tcttgaactc actattcaag
     2941 caggatattt caaaaacagg aatttggaca acgagtacat atacggctgc tatcgatagc
     3001 gaaagtaagt atcttggaca caaggctctt aaaattatag gtctgaatcc atctggccgt
     3061 gatggaggta atcccaaggt tacttatcca g
//
LOCUS       DAVLFB010000144         3000 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLFB010000144 DAVLFB010000000
VERSION     DAVLFB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.144"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..268
                     /locus_tag="VXD58_002016"
     CDS             <1..268
                     /locus_tag="VXD58_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV0676649.1"
                     /translation="ILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENYNRL
                     IRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            432..1628
                     /gene="tuf"
                     /locus_tag="VXD58_002017"
     CDS             432..1628
                     /gene="tuf"
                     /locus_tag="VXD58_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV0676650.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1737..3000)
                     /locus_tag="VXD58_002018"
                     /pseudo
     CDS             complement(1737..3000)
                     /locus_tag="VXD58_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          911 a          575 c          641 g          873 t
ORIGIN      
        1 tattctgtcc attactgcag ataatggttc ggagttcaaa cgattgtctg aggtatttcc
       61 tgaggaacat atctactacg cacatgctta ctcttcatgg gagagaggtt caaatgaaaa
      121 ttataatcga ttaattcgga gatggttacc taaaggaacc aagaaaacga ctccgaaaga
      181 agtagctttt atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg actacaagtc
      241 gccaagtgaa tttcttttgg gtggctaact tcaacttgaa atttggggtt aaatttttac
      301 caagaataat tcacaaaaac gttgtaaaac acttgcaatt tagctgaaat ttgataaaat
      361 agtaaggaaa gttagactgt attgcctact gtctatctat aaaatatatt ttattggagg
      421 cttttactca aatggcaaaa gaaaaatacg atcgtagtaa accacatgtt aacattggta
      481 ctatcggaca cgttgaccac ggtaaaacta ccctaactgc agctatcaca actgttttgg
      541 cacgtcgctt gccttcatca gttaaccaac ctaaagacta tgcgtctatc gatgctgctc
      601 cagaagaacg cgaacgcggt atcactatca acactgcgca cgttgagtac gaaactgaaa
      661 aacgtcacta cgctcacatc gacgctccag gacacgcgga ctacgttaaa aacatgatca
      721 ctggtgctgc tcaaatggac ggagctatcc ttgtagtagc ttcaactgac ggaccaatgc
      781 cacaaactcg tgagcacatc cttctttcac gtcaggttgg tgttaaacac cttatcgtct
      841 tcatgaacaa agttgacttg gttgacgacg aagaattgct tgaattggtt gaaatggaaa
      901 tccgtgacct attgtcagaa tacgacttcc caggtgacga tcttccagtt atccaaggtt
      961 cagcacttaa agctcttgaa ggtgactcta aatacgaaga catcgttatg gaattgatga
     1021 acacagttga tgagtatatc ccagaaccag aacgtgacac tgacaaacca ttgcttcttc
     1081 cagtcgagga cgtattctca atcactggac gtggtacagt tgcttcagga cgtatcgacc
     1141 gtggtatcgt taaagtcaac gacgaaatcg aaatcgttgg tatcaaagaa gaaactcaaa
     1201 aagcagttgt tactggtgtt gaaatgttcc gtaaacaact tgacgaaggt cttgctggag
     1261 ataacgtagg tgtccttctt cgtggtgttc aacgtgatga aatcgaacgt ggacaagtta
     1321 tcgctaaacc aggttcaatc aacccacaca ctaaattcaa aggtgaagtc tacatcctta
     1381 ctaaagaaga aggtggacgt cacactccat tcttcaacaa ctaccgtcca caattctact
     1441 tccgtactac tgacgttaca ggttcaatcg aacttccagc aggtactgaa atggtaatgc
     1501 ctggtgataa cgtgacaatc gacgttgagt tgattcatcc aatcgccgta gaacaaggta
     1561 ctacattctc tatccgtgag ggtggacgta ctgttggttc aggtatggtt acagaaatcg
     1621 aagcttaatt cgatttagtt cccagaagaa caattattta agtcagacac taaaagaatc
     1681 ttgcttggca aggttctttt ttggttcttt gtcaactgta gtgggttgaa aaaaaactaa
     1741 gctcgagaaa ggacaaattt cgtcctttct ttttgatgtt cagggcgatg aaaatccgtt
     1801 ttttgaagtt ttcaaagttc cgaaaaccaa aggcattgcg cttgataagt ttgatgagat
     1861 tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg acgattttct
     1921 ctttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc tgcttaagat
     1981 tgtcctcaat gagtccgaaa aatttctccg gttccttatt ctgaaagtga aacagcaaga
     2041 gttgatagag ctgatagtgg tgtttcaagt cttccgaata actcaaaagc ttgtttaaaa
     2101 tctctttatt ggttaagtgc atacgaaaag taggacgata aaatcgctta tcactcagtt
     2161 tacgactatc ctgttgtatg agcttccagt agcgcttgat agccttgtat tcatgagact
     2221 ttcgatgaaa ctgattcatg atttggacac gcacacgact catagtacgg ctaagatgtt
     2281 gtacaatgtg aaagcgatca agaacgattt tagcattcgg gagtgaaaca gtctgggaga
     2341 ctgtttcagc ctgagcctag aaatttgaaa gcgaagctgt ttagctaagt catagtaagg
     2401 actaaacata tccatagtaa taattttgac gcgacatcgg acggctctat catatttaag
     2461 aaagtgatct cggatgacag cttgtgttct accctcaaga acagtgatga tattgagatt
     2521 gttaaaatct tgcgcaatga agctcatctc catctcccga ttgaaacagt cactccccgg
     2581 actgtttcaa cgtcccagga cataatctca ggaagacgcg aaaaatcatg ctcaaagtga
     2641 gaatcattga gcttgcgaat gacagttgaa gttgagatgg aaagctgatg ggcaatatca
     2701 gtcatagaaa tcttttcaat taacttttga gcaatctttt ggttgatgat acgagggatt
     2761 tggtgattct tcttgacgat agaagtttca gcgaccatca tttttgaaca gtgatagcac
     2821 ttgaatcgac gctttctaag gagaattcta gtaggcatac cagttgtttc gaggtaaggg
     2881 atcttagacg gtttttgaaa gtcatatttc ttcaattggt ttccgcactc agggcaagat
     2941 ggtgcgtcgt agtccagttt ggcgatgatt tccttgtgtg tattcttatt gatgatgtct
//
LOCUS       DAVLFB010000145         2963 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLFB010000145 DAVLFB010000000
VERSION     DAVLFB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.145"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..415
                     /locus_tag="VXD58_002019"
     CDS             <1..415
                     /locus_tag="VXD58_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV0676651.1"
                     /translation="RIISVIKISTDIERIGDRIVEILKNLQIIQNNEILKKIISEIKI
                     LHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSTNIEKKIMNYIFEDDGNVS
                     EVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            428..1903
                     /gene="galT"
                     /locus_tag="VXD58_002020"
     CDS             428..1903
                     /gene="galT"
                     /locus_tag="VXD58_002020"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV0676652.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1916..2896
                     /gene="galE"
                     /locus_tag="VXD58_002021"
     CDS             1916..2896
                     /gene="galE"
                     /locus_tag="VXD58_002021"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV0676653.1"
                     /translation="MAILVTGGAGYIGSHIVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKPALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGLPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWN"
BASE COUNT         1076 a          390 c          606 g          891 t
ORIGIN      
        1 aagaattatt agtgtaataa aaatatcaac tgacattgaa cgtattgggg atagaattgt
       61 tgaaatttta aaaaatctac agattattca aaataatgaa attttgaaga aaattatttc
      121 agaaataaaa atacttcatg aagttatcgg actgcatatg aatagggcta tatcttgtta
      181 tagagaggaa caatctggat gtttagatat ggttgttata caaaaacaga atgagataga
      241 agagcttagt acaaatattg aaaagaaaat aatgaattac atatttgaag atgatgggaa
      301 tgtttctgaa gtaattggtg ctttagatat tattcatcac cttgataaaa ttgctcatac
      361 tacgcaatca atttataaat ggataatgta tagaaaatat gggaacataa attagaaaga
      421 aaaatatatg aaaacaatta ttgataattt tgttgataga gtaattgaat acggaatgta
      481 taatgaaatt gacaaaatat atgtgaaaaa tagagtacta gcgttaatag gtgaggaggg
      541 aactgatcgc atttccgatg aaaatgacct gaaacaaata aaagattact tagtggaaat
      601 cgccttaaaa aacggtaaaa ttaaagattt aatcgaggaa aaggaatgtt taggagcaga
      661 gttaatgaat ttcatagttc ctttacctag tcgattaaat gatatttttt ggagttcata
      721 tgatatatca cctcaagaag cagttgaaga gttttataag ttaagtaaag atagtgatta
      781 tattaaaact tctgctattg ctaagaatat tgagtttcga gcatcaacta aatacggaga
      841 gctagagatt acgataaatt tgtcaaaacc cgaaaaagat ccaaaaacaa ttgctgctga
      901 aaaattagta aaagccacta attatcccaa atgtttacta tgtatggaaa atgaaggata
      961 tcaggggcga ataaattatc cagcacgttc gaatcataga attattcgat tgaaacttgg
     1021 tgacgaagta tggggattcc aatattctcc ctacttatac tttaatgagc atgcgatatt
     1081 tttaaatagt caacatgtac caatggctat tacctctaaa acatttgaac aattattgga
     1141 gattgttgat attttgccag gatattttgc tggttcaaat tctgatcttc ctatctcagg
     1201 aggctccatt cttagtcata accactacca gggagggaaa catattttcc caatggaaaa
     1261 agcaaaattt gagagtgaat ttcgttttaa agactttgag gatgttaatg ctggtattgt
     1321 aaaatggcca atgtcagtaa ttaggttaca aagtgaaaat aaaaatcgct tgttagattt
     1381 agctactaaa attctaaata agtggagaga atattcagat ttagaagtag acatcattgc
     1441 tatgactgaa gatgtcccac accatacggt gactcctatt gctcgtaaag tggacgggag
     1501 atatgaatta gatattgttc taagagataa tcatactact gaacagtacc cagatggtgt
     1561 tttccatcct catcaagatg tacaacatat taaaaaggaa aatatcgggt taatcgaagt
     1621 gatgggactt gcgatattac cacctcgttt gaaaccagag ttagaagaag ttggaaaata
     1681 tcttttagga gaggataacg caattgcgga ttatcattta gaatgggccg accaattgaa
     1741 agaaaaatat cctcgcatta ataaagaaga ggttaatagc gtagttcaac acgaagcagg
     1801 acaagtgttt gcgagagttc tggaagatgc aggagtttat aagaatacac cgtctgggca
     1861 tgaagcgttt atgagatttg ttaagtcggt tggaattaat taggaggaaa caaaaatggc
     1921 aatattggta acaggcggag ctggttatat tggtagccat atcgtagtag aattactaaa
     1981 tttaggaaag gaagtcatca ttgtcgacaa cctttcgaac tctagcatct tggtgctaga
     2041 ccgtattgaa gcaattacag gaatacgtcc cgtgttttac gaattagatg tttgtgataa
     2101 accagcattg agaaaggtat ttgaacaaga atcgattgat gctgcaattc attttgcagg
     2161 ttataaagct gtcggagaat ccgtgcaaaa gcctgtgatg tactacaaaa ataatattat
     2221 gagtacattg gcacttgttg aagtgatgtc agaatttaat gttaaaaaga ttgtcttttc
     2281 ttcaagtgcg actgtatatg gaattaacaa tcagtcacct ctaattgaga cgatgcaaac
     2341 aagtgcgaca aatccttatg ggtatacgaa agtgatgctt gagcaaattt taaaagatgt
     2401 tcatgtggca gattcagaat ggagtattgc gttgcttcgt tatttcaatc caattggtgc
     2461 tcatgagtct ggtttgattg gggaagatcc ctcaggactt cctaacaact tgatgcctta
     2521 tattgcacaa gtagcggtag gtaagctatc agagctaagt gtgttcggaa atgattatga
     2581 tacgcttgat ggtactggtg tgcgtgatta tatccatgta gtagatttag caataggaca
     2641 tataaaagct ttagaaaaag tatctgaaaa aacagatgtt tatatctata acctaggctc
     2701 gggagaaggc actagtgtat tacaacttgt aaatacattt gaaagtgtta ataagatccc
     2761 tattccttat aaaatagttc caagacgttc aggagacgtt gcaacttgtt atgcaaatgc
     2821 agacaaagca tataaggaat taaattggag gacaacaaaa tcgattgaag acatgtgtag
     2881 agatacatgg aattagcaat caaaaaatcc caatggctat aattgatgat actgtctata
     2941 tgagtgctgt attttggaaa aat
//
LOCUS       DAVLFB010000146         2933 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLFB010000146 DAVLFB010000000
VERSION     DAVLFB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.146"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            155..1501
                     /gene="gdhA"
                     /locus_tag="VXD58_002022"
     CDS             155..1501
                     /gene="gdhA"
                     /locus_tag="VXD58_002022"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV0676654.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2012..2182)
                     /locus_tag="VXD58_002023"
     CDS             complement(2012..2182)
                     /locus_tag="VXD58_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676655.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2195..2836)
                     /locus_tag="VXD58_002024"
     CDS             complement(2195..2836)
                     /locus_tag="VXD58_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676656.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIQS
                     QGCTQIIIAHRLSTIKDADIIFVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
BASE COUNT          931 a          555 c          491 g          956 t
ORIGIN      
        1 ccaagaaaaa cgttcgtttt tttatctatt ttcaaaaaaa ccaaaaaact gaattgaaag
       61 aatttttaga aaatttctgt tttttcttta cagtgaaaaa aaattctgct ataatgtctt
      121 atgtaataaa atatgacaat aaaaggagaa cgatatgaca tctgctaaag aatatatcca
      181 aagcgtgttt gaaactgtga aagctcgtaa cgggcacgag gctgaattcc tccaagctgt
      241 tgaagaattt ttcaacactt tagaacctgt atttgaaaaa caccctgagt atatcgaaga
      301 aaatatcttg gcacgtatta ctgagcctga gcgcgtggtt tctttccgtg ttccttgggt
      361 tgaccgtgat ggaaaaattc aagtaaaccg tggttaccgt gttcaattca actcagctgt
      421 tggaccatac aaaggtggac ttcgtttcca cccaactgta aaccaaggga tcttgaaatt
      481 cctcggattt gaacaaatct ttaaaaatgt cttgactgga cttcctatcg gtggaggtaa
      541 aggtggatca gacttcgatc ctaaaggtaa aacagatgct gaagtgatgc gcttctgcca
      601 aagcttcatg actgaattgc aaaaacacat cggaccatca cttgacgtac ctgctggtga
      661 tatcggtgtt ggtggacgtg aaattggtta cctttacggt caatacaaac gtcttaacca
      721 atttgatgct ggtgtcttga ctggtaaacc tcttggattt ggtggtagct tgattcgtcc
      781 agaagcaact ggttacggtt tggtttacta tactgaagaa atgctcaaag ctaacggtaa
      841 cagctttgct ggtaagaaag tcgttatttc aggttctggt aacgttgctc aatatgctct
      901 tcaaaaagca actgaactcg gtgcaactgt tatttctgtt tctgactcaa atggttatgt
      961 catcgatgaa aatggtatcg acttcgatct tttggttgat gttaaagaaa aacgtcgtgc
     1021 tcgtttgact gagtatgcag ctgaaaaagc aactgcaacc taccacgaag gtactgtatg
     1081 gacttacgct ggaaactatg acattgctct tccatgtgcc actcaaaacg aaatcaacgg
     1141 tgaagcagct aaacgtttgg ttgctcaagg cgttatctgt gtatctgaag gtgccaacat
     1201 gccgagcgac cttgatgcca tcaaagttta caaagaaaat ggtatcttct acggacctgc
     1261 aaaagctgcc aacgctggtg gtgtagccgt ttcagctctt gaaatgagcc aaaacagtct
     1321 tcgcctctca tggactcgtg aagaagttga tggccgtctc aaagacatca tgacaaacat
     1381 cttcaacaca gctaaaacga cttcagaaac atacggtctt gataaagact accttgcagg
     1441 agctaacatt gctgcctttg aaaatgtagc aaatgccatg attgcacaag gtattgttta
     1501 agatttattt actcaatcct caatcactac gattggggat ttttagcttg aattaaaaaa
     1561 ttggaaaatc taattaaata aaatgaaatg aaatgaaata aaaactgaac aaattgattg
     1621 cggaattcaa accaatttct aacatatttc tagaaattaa tttgactttt ctaatcgatt
     1681 tgttcatatc ttatttcaat ttactatact tacttgaccc tttttatact caatgaaaat
     1741 caaagagcaa actaggaagc gagccgcagg ctgtacttga gtacggtaaa gcaaagctga
     1801 cgtggtttga agagattttc aaagagtatt aaacaaaaag aaaaaccgct actcctaaga
     1861 atagcgattt aatcatgttt ttaagctact actgtagttg ctctattatt taagtgttat
     1921 gaataacttc aatatcttta aagctttttg ttagatttat aatttcaact atagccatct
     1981 ctactcctta gtttacagca aaaaattatg cttaattctt attgtttttg taaacaatca
     2041 gcgaacccaa tactattcca ttcaacaagc ctaaagaaat accatattca actggaaaat
     2101 ctaaaaaaat tgcaaacact attccaatca tcacccctag tgaggctgtt gttaaaatta
     2161 catattcatt ttctttcttc attttcttta cacctcattt cctttttgta tataagctgt
     2221 agtactctcc accaagatcc attaagtact tatgatttcc tgactcaaca atcttaccac
     2281 ctttcattac aaaaataata tccgcatcct taatcgttga caatctatga gctataatta
     2341 tttgagtaca accctgactt tgtatatact ttgttattct ttcctcatta atagtgtcta
     2401 atgcactagt tgcttcatct aaaattacaa tactaggatt atttattaat gcacgtgcca
     2461 gtgctatccg ttgcctttgc ccacctgaaa tatttgaccc catctctgag atgatagtat
     2521 taaatttcat cggcatagcc atgatttcat catagatttg aactgcttta caaacttcct
     2581 ctatcttttg tgaagtaact tcgttcttta aagttatatt atcaagaata cttcggttca
     2641 ataaaaatga atcctgtgga actactccta aattttgact taatattttt ttatctattt
     2701 gattaatatt tacaccatca aataaaattt ttcctgtatc aatcttatat aatcctgata
     2761 gtatttttga taacgtactt tttcctgacc ccgattgacc gactagtgca attctctgtc
     2821 ctggctcaat agtcaaattt aaattttcta ttacaggagc tgagtctttg gaataagaga
     2881 aagataggtc cttaatatct attctacctt ctaaagaaca ctttttaaga cca
//
LOCUS       DAVLFB010000147         2929 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLFB010000147 DAVLFB010000000
VERSION     DAVLFB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.147"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(122..655)
                     /locus_tag="VXD58_002025"
     CDS             complement(122..655)
                     /locus_tag="VXD58_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV0676657.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(744..1520)
                     /gene="recX"
                     /locus_tag="VXD58_002026"
     CDS             complement(744..1520)
                     /gene="recX"
                     /locus_tag="VXD58_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV0676658.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1558..2913
                     /gene="rlmD"
                     /locus_tag="VXD58_002027"
     CDS             1558..2913
                     /gene="rlmD"
                     /locus_tag="VXD58_002027"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV0676659.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          925 a          528 c          537 g          939 t
ORIGIN      
        1 ctctttctag taatgtgttg taactctgct ataatggatt tattcctttt tgtatttaca
       61 caatttattt tatactacca aaaaaacaag gtcaggattt tgttcctgac ctttgacaac
      121 tttaccgatt ctttagttct acatagcgct tgtaccaaat gtttacatag gcttctgaga
      181 aaggaccacg tccattgtta atccaatcaa caagaatttt gacatgttct tttaaaatat
      241 agtccaagtc atcagaataa ttcattttgc gtttgtgacg ctcgtattct tcaacgtcca
      301 agagacgttt ttccccatct gtaaaaattt taacatccaa atcgtaatca atatacttca
      361 gtgcttcttc atccagatag taggggctag ccatattgca atagtaagaa gttccattat
      421 cacgaatcat ggcaatgata ttaaaccaat atttcttgtg aaagtaaaca atagccggtt
      481 ctcgagtgac ccaacgacga ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa
      541 taatggcgtt ttctgttgtt tttagtacca tggtgtcccg ccaagttcgg tggagactcc
      601 catcatgctt ataactttga attgtaataa agtcgccttc ttttggaagc ttcataacta
      661 accaactttc tacaatttat aagtttatca tttactattg taccataaaa ttacctaaaa
      721 tctgtgaatt tcacttggaa atattaaaga tattctctaa gagcgcttgc tatatccgaa
      781 aaatcgtagc cctttcgtgc taaaacttga gttaaacgct gcttcagttc gtatccttca
      841 tactttcgag catacttagt atattgctta tcaagttcct tgaagatgag ttcttgagtc
      901 gtttcttggt caacttggct atctaagtcg tcaaaggcaa ttttagcatc agaataagag
      961 aagcccttgt tagtcaagtt ctggataatc ttatcttgca gggcacgagc tggaagtttt
     1021 ccctcatatt ttttcaatag tttattggct acacgttgag caacttcaga aaaatcaaat
     1081 tctttcaagt tctcttctat agtagatttt gaaatccctt tttgagatag tttctgagtc
     1141 agcacataag gacccttgtc tcctgaaagt tgattggtat tgatgatagc ataagcgtac
     1201 tgaccatcat taatccactt atcttcttta agattagcaa taacttgaga aacgatgttt
     1261 ttatcaatat cgtatttttt cagatattct ctgacttctt tttcagtgcg tgctttaaag
     1321 gataagtggt agagagcgag attcttacca taagaaaatt gagcaaagtc ttgaatctct
     1381 ttcaattcct cttcgcttat caccttatct ctcgataaca taaaacgaac aattgtatct
     1441 tcggtgatat agcatttgtc gccattatca agctccatca ggtagagtct ttttttcttt
     1501 tcaagttttg tgattttcat agctctatta taactcaaaa tgtgataaga taggggtatg
     1561 aatctgaaag tgaaacaaaa aataccatta aaaatcaagc gcatgggaat taacggtgag
     1621 ggaatcggct tttaccaaaa aacattagtc tttgtaccag gagctctcaa aggcgaagat
     1681 atctattgtc agattacttc tattagacgc aactttgttg aagcaaaatt actgaaggtc
     1741 aacaagaagt ctaaatttcg aattgtgcca tcttgtacta tttataatga atgcggaggc
     1801 tgccaaatca tgcacctgca ttatgataag cagctggagt tcaagacgga cttacttcat
     1861 caagcgctga aaaaatttgc tcctgcagga tatgaaaatt atgaaattcg tccaactatt
     1921 ggaatgcagg aaccaaaata ttacagagct aagttacaat ttcagactcg aaaatttaaa
     1981 aatcaggtca aggcgggctt atatgcacaa aactctcact atttagtaga gttgaaagac
     2041 tgcctggtac aagataagga aacccaagtg attgctaatc gcttagcaga attacttact
     2101 tatcaccaga ttccaatcac ggatgagaga aaagttctag gtgtccgtac tattatggtc
     2161 cgacgcgcga gaaagaccgg acaggttcag attattattg ttacaaaccg ccagcttaat
     2221 ttaactcaat tggtaaaaga gttggttaaa gatttcccag aagttgtgac agtagctgtt
     2281 aatacaaata cagctaaaac cagtgagata tatggtgaaa agacagagat tatctggggg
     2341 caagagagta ttcaagaagg tgtactcaat tatgaatttt cactatcccc tcgagctttt
     2401 tatcaactaa atcctgagca aacagaagtc ctctatagcg aagcagtaaa agcgctggat
     2461 gttgataaag aagaccattt gattgacgct tattgtggag ttggaacgat tggatttgcc
     2521 tttgcaaaga aagttaaaac actcagaggt atggatatta ttccagaagc tattgaagat
     2581 gccaagcgaa atgctaaaag aatgggattt gacaatactc attatgaagc tggaacggca
     2641 gaagagatta ttcctcgttg gtacaaggaa ggctaccgag cagatgcttt gattgttgac
     2701 ccaccacgta caggtctgga tgataagtta ttagatacta ttcttactta tgtaccagaa
     2761 aaaatggttt atatttcttg taatgtttcg accttggctc gtgatttggt acgcttagta
     2821 gaagtctatg atcttcatta tatccagtcg gtcgatatgt tcccacatac agctcgaact
     2881 gaagctgttg taaaattaat aaaaaaagtt taaaaaagta gttgacaaa
//
LOCUS       DAVLFB010000148         2901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLFB010000148 DAVLFB010000000
VERSION     DAVLFB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.148"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..237)
                     /locus_tag="VXD58_002028"
     CDS             complement(<1..237)
                     /locus_tag="VXD58_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676660.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYQVPILKNEYTVKLLLLME
                     CRIEDQEVIEYLVSTWKYRDLVLHSVRMYEIEKSE"
     gene            complement(469..1425)
                     /gene="lytA"
                     /locus_tag="VXD58_002029"
     CDS             complement(469..1425)
                     /gene="lytA"
                     /locus_tag="VXD58_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV0676661.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLRAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1429..1752)
                     /locus_tag="VXD58_002030"
     CDS             complement(1429..1752)
                     /locus_tag="VXD58_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV0676662.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKDYLVQKGGEKTIKIVEILA
                     KNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQMND
                     AWKGR"
     gene            complement(1756..2055)
                     /locus_tag="VXD58_002031"
     CDS             complement(1756..2055)
                     /locus_tag="VXD58_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676663.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHAVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(2065..2415)
                     /locus_tag="VXD58_002032"
     CDS             complement(2065..2415)
                     /locus_tag="VXD58_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676664.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGHRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(2418..2621)
                     /locus_tag="VXD58_002033"
     CDS             complement(2418..2621)
                     /locus_tag="VXD58_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676665.1"
                     /translation="MNTEQLNQALQMTIREISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(2716..>2901)
                     /locus_tag="VXD58_002034"
     CDS             complement(2716..>2901)
                     /locus_tag="VXD58_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV0676666.1"
                     /translation="KHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIK
                     AIQELNQKIEKMEKTIA"
BASE COUNT          704 a          669 c          538 g          990 t
ORIGIN      
        1 ctcactcttt tctatctcat acatccttac cgaatgcaac acgagatcac gatacttcca
       61 agtgctgacc agatactcaa taacctcttg gtcctctatc ctgcattcca taagtaataa
      121 tagtttgacc gtgtattcat tcttcaaaat cggaacctga taagtcacat ctacccaatg
      181 ctcaaaacct aagtctgtct gctctacatt tgctagttca atatttaaaa tcttcatttt
      241 tattcctcct acttatctat tcgtaaaaga agataaaaag ttatgaaaaa atcattactt
      301 ttttaattca gacagtactt tcagagcaat tttgttaatg tcaacaaaat tggcaaccaa
      361 gcgatttaat actatttgtt gacgccaaca agtcaatttt cagacaaaca aaaaaaccgc
      421 aagcctgagc ctgcggtgaa agaacaattt agaaagtttc ctttctattt atttaactgt
      481 aatcaagcca tctggctcta ctgtgaactc tggcttatct gccagtgttc cgtctggttt
      541 gaggtagtac cagcctgttc cgtctgcgga ctggacgaag gcgtttgata ccatagcgcc
      601 ttctttagcg tctaagtagt accaagtgtc cttgtacttg acccagcctg tcttcatggc
      661 accttcttcg ttgaaatagt accacttctc agcaattttc ttccagcctg tagccatttc
      721 gcctgagttg tcaaaccagt accagttgcc gtctgtgtac ttcttccagc ggtctgcaag
      781 catatagcct gagccatcga aataatacca ggtaccgttg attttctcaa acttatcttt
      841 tggataagag ccgtctgagt gtacgtacca gtagccagtg ccatttttct gccagcctgt
      901 tgcagctctc aagccgtttt cgatgtcttg cttaaactgt tcacggctaa tgccccattt
      961 agcaagatat ggatatggat ccacatggtc tgagtggttg tttggttggt tattggtaca
     1021 gtattcatgc gtcttgatac ctgccaagtc gtctgtatca agagtcttcg gcaagcctgc
     1081 ttcgtccgct agattgcgta gcaattcgat atagaggcgg tagtccgtca tgaactcttc
     1141 tttagttgaa tggctttcaa tcagttcaac cgctgcgtaa ctctcagcat tccaaccgcc
     1201 cccaacatcc caacttccgt tgttcacagg tcctacctgc atgatgcgac cgttcccaac
     1261 aacgtgcgag aaaaaaccta attctgggtc tttccgccag tgataatccg cttcattctg
     1321 tacggttgag ttacggttgc ctgttgaatg ggcgtgtact tgtcgataag gctgcacccc
     1381 aacctgtggc aagcctgtac gtagtctgtt tctatcgata tccattccct atcgtccttt
     1441 ccatgcgtca ttcatctgct tcactgctga ctctacgaag gtgtctaagt ctttgtcagt
     1501 catgctaata ttgtattttg taagctcagc acggacttta gcgcgagcct gtgccagttt
     1561 ttcatctccc ttgtagccag tttcagcagc tacttgctca acggcattta ctgcattttt
     1621 ggccaagatt tcaacgattt tgatggtctt ttctccacct ttttgaacca ggtagtcctt
     1681 gactgcgata ccagccaaaa taacaaggat gctgattgct ccattagtaa tgatttcagt
     1741 aatttgttgc atttgttatt ctcctttttc gatttcttcc atgcggtcgt tcatgcgaac
     1801 catttctttt tgaatgtctc cgaccgcgtg agtgattgtg gttaattctg tagtggtctt
     1861 ttctaggtga gtcatcaaac gctcttctcg tctattagag tcggcctttg attgctcgtg
     1921 caaatccata atcttcttct ctcgcttgtc cgaagtcttg ataagatatc gaatgataat
     1981 aaagaaaagc aagataaaca aaatcgccca agctacctga ctttgagcga ttttttcagc
     2041 ttcttcaatt ggcatacaca cctcctattc tttaggtttt accgttggat ccgtccagtc
     2101 aggattgccc tctgcatcaa atttcatgat atagaattca tgattcaaca gaacggctac
     2161 gttgattgtt gcgattgtac caccccactg gttgaacgcc caaacggttt caacatcctt
     2221 taattggcga tggccattta cgatcacagg acgtttttga acatcacgat acatatagaa
     2281 gtcatcgctt acattcttgc aacgaatgaa ctctccattt tctttcatgt agcgcaaagc
     2341 gctcgcaaga tcaaatggtt ctgtgatttt tgtaaggtct agtaagttat ctgtgttttg
     2401 aattgtttct gccatgtcta ttctcctttg tcagctggtt tagtttgttc atcaagcaga
     2461 gcttccagct catccactcg tgcttgaagt ctttgattct cttccctttg ctcatccaac
     2521 tgaatactca agatattact tgtaatcatc gaatttgttg aagttgttga tatttcacga
     2581 attgtcattt gtaaggcttg gttaagctgt tctgtgttca ttttctaagt tctccaatct
     2641 gtgtgttcgt tttctatttt caagagcaag ctcctaaatt gccttaaagt gcgatattgg
     2701 tcaacatgtt gttattcatg ctattgtttt ctccattttt tctatttttt gatttaattc
     2761 ttgaatagcc ttgattaagt aaggaactaa agcggtatag tcaatatgca gatagccatc
     2821 tggattctca ggatctcgtg agacaattct tggaacgatg gtttcagcct cttgagctat
     2881 tagaccaatc tcctcatgtt t
//
LOCUS       DAVLFB010000149         2842 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLFB010000149 DAVLFB010000000
VERSION     DAVLFB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.149"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..2805)
                     /locus_tag="VXD58_002035"
     CDS             complement(<1..2805)
                     /locus_tag="VXD58_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0676667.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVIVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     KVEPEYAGVQAGAIVEPEQVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPE
                     KVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEPSKEYTGVQAGAIVE
                     PEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEMSIQ
                     KKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDKSKN
                     VYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNYSKV
                     NLNDKLNVHGYPARKYTHLKDATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEV
                     IGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEV
                     IKKLELRNISSVELYSKDGNK"
BASE COUNT          703 a          610 c          453 g         1076 t
ORIGIN      
        1 cttattacca tcttttgaat atagttctac agaagatata tttctaagtt ctaatttctt
       61 aataacttct ttttcatctt tagcgtcatc ttttttctca tctttatgac gataaagttc
      121 atcgtatact ggtggaattt cattacctgc tataatactc ttaatccaag ctaattgtgt
      181 aggagataaa atcaatccgc ctgagcgatt ttgtgctcca ttttgatgca caccaataac
      241 ttcaccatta gcattaaata ttcctccacc actcatacca ggatttccac cttgatattg
      301 aacaccgtag gtgttattcg catattcttg ttccatttct accgtagcat cttttaagtg
      361 cgtatatttc ctagctggat atccatgaac atttagtttg tcattaagat ttactttcga
      421 ataattttta actaattcta catgttctct gccagttgtt tcaggtagct taatcactgc
      481 taaatcatag ataaatcctt ttggataatc ttttttatta taaaaatgaa tattgttagc
      541 agtaaatgtt cctttttgtc catcaggagt cacccactca tatacgtttt tagatttatc
      601 acctctaaat tcgccagtag tattatcagt agaattatta atgaaattat gtgctactgt
      661 taaaagtaca tttggtgcaa taaaagtagc tgatcctact ccagtaacct tcgacgaatc
      721 tgtaataaag ttcatattaa ttaatgcact agattttttc tgaatactca tttcttctgg
      781 tgtattagta ttttcattat ttggtttagt atcttctgca ctaggttgtt cgattttacc
      841 agtatattct ttcagtgctt ctactttctc tggctcgact atcgctccgg cttgcacccc
      901 tgtgtattct ggtagtggtg ctatctgctc tggttcaaca atagcgccag cctgcacccc
      961 tgtgtattct tttgatggtt ctactttttc tggttctact attgctccgg cttgcactcc
     1021 tgtgtattcc ttcggtgctt ctaccttctc tggttcgact atcgctccag cctgcactcc
     1081 tgtgtattcc ttcggtgctt ctactttctc tggctcgact atcgctccag cctgcactcc
     1141 tgtgtattcc ttcggtgctt ctactttctc tggctcgact atcgctccgg cttgcacccc
     1201 tgtgtattct ggtagtggtg ctacctgctc tggttcaaca atagcgccag cttgtactcc
     1261 agcatattcc ggttcaactt tttcaggttc aacaatagct ccagcttgta ctccagcata
     1321 ttccggttca actttttcag gttcaacaat agctccagct tgtactcctg tatattctgg
     1381 tagcggttct acctgctctg gatcaccctt gtcagtcatg actgcctctg gtaacgctgg
     1441 ttgaacttct ggtacgcctt tatcacttac tacagcttct ggcaactctg gttggactgc
     1501 tgactcacct ttgtcggtta atacagctgc tggtagttca ggctgaattg cgggttcaac
     1561 aatagctcca gactgtactt ccttatgttc tgcaccagtc tcagcttgtt cctttataac
     1621 ttgagctttt ttagtacctt tttcgactat tcttgaaaca ggcgcagtcg ttgaagttga
     1681 aataatttct cgtgaaactt cttcattgtt tacagaaaat attctgacga tttcaacttt
     1741 cttacctaat gcaccttctt gttttactat tacagttcct tctgctaaat caggattttc
     1801 ttgaatttct tcttgaaaat ctatttttgt ttctatagtt tcgtcacgat ataagagttc
     1861 cggtttgttc aactgacctg atagcacttc atcctgtgga tttaatgtat ttaccccagt
     1921 cttttccttt ggagaaatct tctcctcttt cttcgtttct agattcttat gttcggctaa
     1981 ttgttcttga gaatctgaag attgtttctc ttcttttctt ggattgatta attcagtaga
     2041 gaaaggtttt tcaactactt gaacttctgt cggcttagtt gaagaaacag gtgtttgttc
     2101 ctgaatagct tgtactgttg atggatggtc tacaaaattt ggtgtaacat tataatccac
     2161 cttttgttgt tttgtaggag tggctgctga tttttcttga ttacttactt caaaatcaga
     2221 agtcgttttt ccctctttga tatatccaat ataagtgtaa cctgaaatct ctttaggaag
     2281 aggtaatttt tctccagagg tcaattcata gtccgtattg taatttagca aaagatgatt
     2341 ttctaaagca tggactgaaa ctaagacacc atttcccatt cccgcaacca atactaaatg
     2401 taataccgtt ttattcttaa cctttttctt ggaaacagca aaaattaaaa ttcccagagc
     2461 agctaagcta gcaccagcaa ctagggcttg cctctcattc ttgcttccag tatttggtaa
     2521 ttccgccagt tgattttgag aatttaactt ataaacaaga taataagttt catcatcatt
     2581 ctccacgtat gtcggaatat catagacaag ctgcttcttt tcttctgatg atagctctga
     2641 atctgccaca tatttatagt gaactcccgc agtttcttga gcatccacag atgaactagc
     2701 taatacagac ataaaaaata aacttgaaat cgttgcagat acaagtccta ctgataattt
     2761 tctaaatgaa aaacgctctt gtttttcacc aaaatacttt tccattattc ctccttgaaa
     2821 taaaatttat atatgttaca aa
//
LOCUS       DAVLFB010000150         2697 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLFB010000150 DAVLFB010000000
VERSION     DAVLFB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.150"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            446..2605
                     /gene="pfbA"
                     /locus_tag="VXD58_002036"
     CDS             446..2605
                     /gene="pfbA"
                     /locus_tag="VXD58_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV0676668.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
BASE COUNT         1049 a          334 c          515 g          799 t
ORIGIN      
        1 tataatttac aacatatcaa caaatttaaa tatagtaaat gggatatttt atattcaagc
       61 taagaaagat agcatcactt ttgaatggaa ggctaaagag caaactagga agttggccat
      121 agatagctca aaaccctgct ttgaggttgt agatatagta aaatgaaaca agaataggac
      181 aaattgatcg ggacagttaa atcgatttct aacaatgttt tagaagtaga ggtgtactat
      241 tatagtttca gtctactata gaactgacca agtcagtaac ctagacttag ggcaaggcgg
      301 cactgaccta gtttgaagag atttccgaag agtataaatt ttaatatttt cttgtgttat
      361 tccttgacaa ttcaatttgg aaaatatatg ataaagataa tgacagcggt gtcattctat
      421 ctattttaag aaaagtaata atcaattgtt aaaaatagta aaaaaattgg aggttctgat
      481 gaaatatttt gttcctaatg aggtattcag tattcgtaaa ttaaaggtgg ggacttgctc
      541 ggtactattg gcaatttcaa ttttgggaag ccaaggtatt ttatcggatg aagttgttac
      601 tagttcttca ccgatggcta caaaagagtc ttctaatgca attactaatg atttagataa
      661 ttcaccaact gttaatcaga atcgttctgc tgaaatgatt gcctctaatt caaccactaa
      721 tggtttagat aattcgttaa gtgttaatag tatcagctct aatggtacta ttcgttccaa
      781 ttcacaatta gacaacagaa cagttgaatc tacagtaaca tctactaatg aaaataagag
      841 ttataaggaa gatgttataa gtgacagaat tatcaaaaaa gaatttgaag atactgcttt
      901 aagtgtaaaa gattatggtg cggtaggtga tgggattcat gatgatcgac aagcaattca
      961 agatgcaata gatgctgcag ctcaagggct aggtggagga aatgtatatt ttcctgaagg
     1021 aacttattta gtaaaagaaa ttgttttttt aaaaagtcat acacacttag aattgaatga
     1081 gaaagctaca attctaaatg gtataaatat taagaatcac ccttccattg tttttatgac
     1141 aggtttattt acggatgatg gtgcgcaagt agaatggggc ccaacagaag atattagtta
     1201 ttctggtggt acgattgata tgaacggtgc tttgaatgaa gaaggaacta aagcaaaaaa
     1261 tctaccactt ataaattctt caggtgcatt tgctattggg aattcaaata acgtaactat
     1321 aaaaaatgta acattcaagg atagttatca agggcatgct attcaaattg caggttcgaa
     1381 aaatgtatta gttgataatt ctcgttttct tgggcaagcc ttacccaaaa cgatgaagga
     1441 tgggcaaatc ataagtaagg agagcattca gattgaacca ttaactagaa aaggttttcc
     1501 ttatgccttg aatgatgatg ggaaaaaatc tgaaaatgtg actattcaaa attcctattt
     1561 tggcaaaagt gataaatctg gggaattagt aacagcaatt ggcacacact atcaaacatt
     1621 gtcgacacag aacccctcta atattaaaat tttaaataat cattttgata acatgatgta
     1681 tgcaggtgta cgttttacag gattcactga tgtattaatc aaaggaaatc gctttgataa
     1741 gaaagttaaa ggagagagtg tacattatcg agaaagcgga gcagctttag taaatgctta
     1801 tagctataaa aacactaaag acctattaga tttaaataaa caggtggtta tcgccgaaaa
     1861 tatatttaat attgccgatc ctaaaacaaa agcgatacga gttgcaaaag atagtgcaga
     1921 atatttagga aaagtatcag atattactgt aacaaaaaat gtaattaata ataattctaa
     1981 ggaaacagaa caaccaaata ttgaattatt acgagttagt gataatttag tagtgtcaga
     2041 gaatagtata ttcggtggta aagaaggaat tgttattgag gattcaaagg gtaaaataac
     2101 cgttttaaat aaccaatttt ataatttatc cggtaagtat atatcattca tcaaatctaa
     2161 tgcaaacggg aaagaacctg ttatacgtga tagcgatggt aatttcaata ttgtaacgga
     2221 gaatgggctt tacaaaattg taacaaataa tttaagtgat aaaaacgaaa aagaaaaaaa
     2281 caaagaggaa aaacaatcta attcaaataa tgtaattgat agtaaccaga agaacggaga
     2341 gtttaactca agtaaagata atagacaaat gaatgacaag atcgacaata aacaagataa
     2401 taagacagaa gaagtaaact ataaaatagt tggagatggc agagaaactg aaaatcatat
     2461 taataaatct aaagaaatag tagatgtaaa acaaaaatta ccaaagactg gttcgaacaa
     2521 gattatggaa ctattcttaa cagtgacagg aattggttta cttttgacac taaaagggtt
     2581 gaagtattat ggtaaagata aataaaattt gttcgataca aggaagttcc gtagaaaatg
     2641 aggatattgt aggttcacaa aatcagtatt tttggattat tggtggagct acagatt
//
LOCUS       DAVLFB010000151         2658 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLFB010000151 DAVLFB010000000
VERSION     DAVLFB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.151"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            64..228
                     /locus_tag="VXD58_002037"
     CDS             64..228
                     /locus_tag="VXD58_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV0676669.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKENKDKFLSILTSLAGLALV
                     LVAVWLGWPK"
     gene            307..1035
                     /gene="yaaA"
                     /locus_tag="VXD58_002038"
     CDS             307..1035
                     /gene="yaaA"
                     /locus_tag="VXD58_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV0676670.1"
                     /translation="MKILIPTAKEMNTDLPSIETTLLRSESQAVLDALALYSASQLES
                     FYKVSAEKAAEECQNIQALKRQTAQHYPALKLFDGLMYRHIKRDKLTEVEQAYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDEAVKQEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1086..2570)
                     /locus_tag="VXD58_002039"
     CDS             complement(1086..2570)
                     /locus_tag="VXD58_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV0676671.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT          869 a          414 c          545 g          830 t
ORIGIN      
        1 aaaggtttat ttaagaaata tatgttatag taaaataaat taagactaat ggaggacaaa
       61 gaaatgggtt tttatttgat ggttgcttca atgctactcg gactgctcgc tttgaagata
      121 ggtttttctc agttcaagga gaacaaagat aaattcttat ctatcttaac aagtttagct
      181 gggctagccc ttgttctcgt ggctgtctgg ttaggatggc ccaaataaat agaaaaacct
      241 cggtcaaacc gaggtttttc agatgaattt cttggttgtg gctaaaaaat atgctacact
      301 atcaatatga agattttaat ccctacagca aaagaaatga atacagactt gccgagtatc
      361 gagacaactc ttttaagatc agaaagtcag gctgtacttg atgccttggc tctctattct
      421 gccagtcaat tggaaagttt ctacaaggta tcagctgaga aggctgcgga agaatgtcaa
      481 aatatccaag ctttgaaaag gcaaactgct caacactacc cagccttgaa gctttttgat
      541 gggcttatgt accgtcatat caagagagac aaactgaccg aggtagaaca agcctatctt
      601 gaaaatcatg tcttcattac ctcggctttg tacggagtcg ttccagcctt gtcgcctatg
      661 gcacctcacc gtttggattt tttgatgaaa ttaaaagtcg ctggtaagac tttgaagagc
      721 cattggaagg cagcctatga tgaagctgtg aagcaggaag aagtgatttt ctctctcttg
      781 tcatcagagt ttgagactgt attttctaag gaaatcagag caaagatggt gaccttcaaa
      841 ttcatggagg atagaggcgg tcagctgaag attcactcaa ctatctccaa gaaagcgcgc
      901 ggggcctttc taacagcttt aatagaaaat caagtacaaa ctgtggggga agcacgtcgc
      961 ttgaactttg ctggatttgt ttaccgagaa gatttgtcac aaccacaggg attggttttt
     1021 gtaaaggaag tatagaaatc agataagaga aaagtcagca cgattatgct gacttttttg
     1081 ataacttata aagtattgga caaatgaatc tcatttgata aggtgttaag atgattaacc
     1141 aaattcatga ttgacagatg atttcggcaa ataaacttct tggtaatccc tgaaatatag
     1201 aaattagaca cgatgatatc gtaatctgtc tgatttaaaa tctcaggact tgtttttaat
     1261 tcatcccaaa tatcgaaggt aaagcggtta ttacagtaat aggaaagcat atcaacaaag
     1321 gtaagggata tagcatggtc aaagttactg ataatcaaaa ccttgatagg tggtcgattt
     1381 tctaacaact gagtagagag gttttcagca tgggttaaga tggtgtacat caagtgttcc
     1441 aactgttcag gatggtcatg ctgtccaatt gcctgacggt attgagccag ttcttgacgc
     1501 gcacttccca taaagtctgg gaagtaaact tcaaattttt taatcgtcaa tgctttttgt
     1561 tcaaataaga ttggggtaga aaagatttcc tgtctttcaa agaaagcagt gttgtgtaaa
     1621 tgccaaatca gctcatcgtg gttagtaaaa gtaatcttat attgttttga taggcgttct
     1681 aatatttgac taagtaattg gtaagagtat cttgcttgac tgttatcttc taaagagttg
     1741 aagaattctt gaggatctag gaaaatatca ttctgaagaa aagaaataaa aatttgagcg
     1801 attgtatctg gagtcatttc aagcccaaaa tgtttagaga aataagcaag tgtctcctgg
     1861 aaatttggaa tgctctttaa aagaggataa tattctttgt aaaagtggtt ggggaggtca
     1921 atgaagtgac catttttaac cctgtggata ctgatagcta tcatcagttt gtacattcta
     1981 taaattgaaa atcgcattgg ataattcgtg attttataga agaagtcagc aaactctgtt
     2041 aaatcttcct caggaaggtc aggaaaaggc caatctagga aataatagcg ctcagaaaag
     2101 tattgggcaa aaaagtaacg aatatcgatc tcatttccac gtatctcgga tggagtaaaa
     2161 gataattcaa tttgaaactg agatgaaaga acctttgtaa tatttctgcc cagtcgatag
     2221 aggttggcac tactaaagta aagattttct attgttcgat aaataggtag cccctcattg
     2281 aagaacatat actctagtaa ttgaaaagat tgagaattag caagaaaata ttgataaata
     2341 tcttctacac tagtgttaac ttctaaatct atcatgattc cgttaacaga agattggatg
     2401 ttaatagatg gaaaggcaat acgtaattca tttaaatcac tttttaagat tcgttctgta
     2461 catcctaatt tttcggctag ttcatgtaat ttcatccaat tacgattctg aattagggtt
     2521 tccattaagc gtaattgtct ttgaactttt gtggataaaa ggtttctcat acgggtattt
     2581 cctccataat tcattatatc tttttttgtc taagtagtat aaaaaaatta gagcaagaaa
     2641 attgtcgtta gtaatcgg
//
LOCUS       DAVLFB010000152         2613 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLFB010000152 DAVLFB010000000
VERSION     DAVLFB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.152"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            136..1338
                     /locus_tag="VXD58_002040"
     CDS             136..1338
                     /locus_tag="VXD58_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV0676672.1"
                     /translation="MKILFVNMFYNEAGGAQKSTKFLAEQLVKEGHEVFAYSDDAIDS
                     DIDEVINRVKIFRRRTPLFSLHYIFSAKKNPAKHFFYKIFETYNVFAKRKFINIIEEV
                     QPDIVHFNTISGMSLSIVKEAKKRNIRTVWTLRDYWLEYPWGEKDISLIKLLNRIYRP
                     FVKKSLRHLDIVTAPSAFTLNNFINKGFINPSSQKCMVIENAVIFSKGKLENIIKQKQ
                     ETKRNPEKTNFLFVGSILEIKGVFNLVKAFKKLTSPEISLKIVGKGKDLEKLQKEIES
                     DPRIQYLGFQDTETLYKTYQNSDVLVVPSAWDEPFGRVVIEGNANGLPVITSDRGGLP
                     EIVQTVGGGEIFTDESGNTLYSLLKKFVEMENYNVYYQCILENIDIYSIEKQSEKFTN
                     LYLDKEIE"
     gene            1372..2604
                     /locus_tag="VXD58_002041"
     CDS             1372..2604
                     /locus_tag="VXD58_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV0676673.1"
                     /translation="MKIAVAGTGYVGLSIAVLLAQHHEIKAVDIVAEKVELINQKKSP
                     IQDDYIEKYLVEKDLNLEATMDAKYAYEDAEFVVVAAPTNYDSKKNFFDTSAVEAVIQ
                     QVKKINPKAVIIIKSTIPVGFTESVQKKYQYKNILFSPEFLRESKALYDNLYPSRIIV
                     GTDLEDTYLKDKANIFASLLKEGAIKEDIDTLFMGVTEAEAVKLFANTYLALRVSYFN
                     ELDTYAEVKGLDAKAIIDGIGLDPRIGNHYNNPSFGYGGYCLPKDSKQLLANYFDVPQ
                     NMMTATVESNRTRKDFIAQRILEKAGVSETDSLDAFKNIQDIVIGIYRLTMKSNSDNF
                     RHSSIQGVMKRLKAKGVTVIIYEPTLKDGETFFGNKVVNNLDKFKEASNVIVANRFEP
                     SLEDVSNKVYSRDIFKRD"
BASE COUNT          952 a          348 c          492 g          821 t
ORIGIN      
        1 gattttgtaa acgaccttat tttacattta tcagttcagt ttgttatttt atttttaata
       61 tggggaattg ggatgtatat tagcggagag tatcgacgag tcttcgcggt tataagaaaa
      121 taaaggagca attttgtgaa aattttattt gtgaatatgt tttataatga agcaggaggt
      181 gcacaaaaga gcaccaaatt tctagctgaa caacttgtaa aagaaggaca tgaggtattt
      241 gcatactctg atgatgccat tgattcagat attgatgaag ttattaatag ggttaagatt
      301 tttagaagaa gaaccccgct ttttagtctt cactatattt tcagtgctaa gaagaatcca
      361 gctaaacatt ttttctataa aatatttgaa acctataatg tatttgcgaa aagaaaattt
      421 atcaatatca ttgaggaagt acaaccagat attgttcact ttaatacaat ttcaggaatg
      481 tctttatcaa tagttaaaga agctaaaaag agaaatatca ggaccgtttg gaccttgcga
      541 gactattggc tagaatatcc atggggtgag aaagatattt ctcttatcaa acttctaaat
      601 agaatatata gaccttttgt taagaaaagt ttaagacact tagacatagt aacagcacca
      661 tcagcattta ctttgaataa ttttatcaat aaaggattta ttaacccatc ttctcaaaaa
      721 tgtatggtca ttgaaaatgc tgttatattt tcaaagggta agttggaaaa tattatcaaa
      781 caaaagcaag aaacaaaaag aaatccagaa aaaacgaatt ttctttttgt aggttctata
      841 cttgaaataa agggcgtttt taacttagta aaggccttta aaaaactaac tagtccggaa
      901 atttctttga aaattgtagg aaaaggaaaa gatttagaaa aacttcaaaa agaaattgag
      961 agtgatccta gaatacaata tttaggcttt caagatacag aaaccctcta taaaacttat
     1021 caaaattcgg atgtattggt cgtcccatct gcttgggatg aaccgtttgg tagagttgta
     1081 attgaaggaa atgcaaatgg ccttcctgtc atcacttcag atagaggtgg attacctgaa
     1141 attgtgcaaa cagtaggagg aggagaaatt tttacagatg aatcgggaaa tacattatat
     1201 agtcttttaa agaaatttgt agaaatggaa aactacaatg tctattacca atgcattctt
     1261 gagaatattg atatttattc tatcgaaaaa caaagtgaaa agttcactaa tctgtattta
     1321 gataaagaaa tcgaataata agttgttttt gtagcagtaa cggaggtaat tatgaaaata
     1381 gcggtagcag gaacaggtta tgtaggatta tcaattgctg tgttgttggc acaacatcac
     1441 gaaataaaag cagttgatat tgttgctgaa aaagttgaat taatcaatca aaaaaagtct
     1501 ccaattcagg atgattatat tgaaaaatat ttagtggaaa aagatttaaa tttggaagca
     1561 actatggatg ctaagtatgc ttatgaagat gcagaatttg ttgttgttgc agcaccaaca
     1621 aattatgatt cgaaaaagaa cttttttgat acatcagcag tagaggcggt cattcaacaa
     1681 gtaaaaaaaa ttaatcccaa agcggtaata attattaaat ctacaatccc tgttggattc
     1741 acagagtccg ttcaaaagaa atatcagtat aaaaatatct tatttagtcc agaattttta
     1801 cgcgaatcta aagcattata cgataacctg tatccaagtc gcatcattgt tggaactgac
     1861 ttagaagata cttatttaaa agacaaagct aatatatttg cttccctact aaaagagggg
     1921 gctattaaag aagacataga tacacttttc atgggagtaa cggaagctga ggcagttaaa
     1981 ttatttgcca atacctacct tgcgttgcgt gtttcttact ttaatgaatt ggatacttat
     2041 gcagaggtga aaggtttgga tgcaaaagcc attattgatg gtattggcct agatccacgt
     2101 ataggaaatc attataacaa tccatccttt ggctacggtg ggtattgttt acctaaagat
     2161 agtaagcagt tattagcaaa ttactttgat gtaccacaaa atatgatgac agcaactgtt
     2221 gaaagcaatc gaacgcgtaa ggattttatc gctcaacgga ttttagaaaa agctggagta
     2281 tctgagactg atagtttaga tgcgtttaag aacatacaag atattgttat aggtatttat
     2341 cgtttaacaa tgaaaagtaa ttcggataac ttccgtcact cctctattca aggcgttatg
     2401 aaacgattga aagctaaggg agttacggtt atcatctatg agccaacttt gaaggacggg
     2461 gaaacattct ttggtaataa agtggtaaat aatctagata aatttaaaga agctagtaat
     2521 gttattgtag ccaatcgttt tgaaccttca ctagaggatg tgtctaataa agtttatagt
     2581 cgagatatat ttaagcgtga ctaataaata cgt
//
LOCUS       DAVLFB010000153         2558 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLFB010000153 DAVLFB010000000
VERSION     DAVLFB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.153"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..376)
                     /locus_tag="VXD58_002042"
     CDS             complement(<1..376)
                     /locus_tag="VXD58_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV0676674.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKK"
     gene            complement(469..627)
                     /locus_tag="VXD58_002043"
     CDS             complement(469..627)
                     /locus_tag="VXD58_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV0676675.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(698..1127)
                     /locus_tag="VXD58_002044"
                     /pseudo
     CDS             complement(698..1127)
                     /locus_tag="VXD58_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1222..1662)
                     /locus_tag="VXD58_002045"
     CDS             complement(1222..1662)
                     /locus_tag="VXD58_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0676676.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1653..1961
                     /locus_tag="VXD58_002046"
                     /pseudo
     CDS             1653..1961
                     /locus_tag="VXD58_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2116..2439)
                     /gene="tnpB"
                     /locus_tag="VXD58_002047"
     CDS             complement(2116..2439)
                     /gene="tnpB"
                     /locus_tag="VXD58_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0676677.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
BASE COUNT          772 a          530 c          481 g          775 t
ORIGIN      
        1 tttttttagg gcgattgatt tttacttccg tcgcatcaat cattaccgtg tcctcagaac
       61 tgagaggagt tcttgaaatc gtaacaccac tttgaacaag agttacttca acccattggc
      121 tccgacggat taagttgctt tcgtgaatac caaaaacagc cgcaatttgt tcataagttc
      181 gatattctcg cacatattga agagtggcca taagaaggtc ttctagactt aatttaggtt
      241 ttcgtccacc ttttgcgtgt ttaagttgat aagctgtttt taatacagct aacatctctt
      301 caaaagtcgt gcgttgaaca ccaacaagac gcttaaatcg tgcatcggtt agttgtttac
      361 ttgcttcatc attcatagaa ctattatacc atattttgtt tcgcaggaag tctattgaaa
      421 aatctccaga ctggagaact cacggatagt tcctaatctg gagatttctt atttgcactt
      481 ttcttgtaca actttagtcc atggtaaata agcctctaaa acctctttgt ttacgagagt
      541 ttcctcgttt ggaagacatt ctagaagata ggatagatat ttctcgctat ttaattgatg
      601 acgtttagct gtttccaaca aactcataat aatagctctt gctttagctc cttcaaaact
      661 ttgagaaaat aaccaccctc gcttttttaa actgagctta ggctaaaaga gtccactgga
      721 ctcttttact ccgtcccata accaatgatt taatggcgcg ttcagctaga ttattggaaa
      781 ggaccagatg tccgtctttc aaaatggtct taaaggtttc ttcatacttg aggctgtatt
      841 caattgccct tcctagtttt gaacctgcta aaactgactg acgccggcac taagcaaaga
      901 agtcttccat taagggctgg agatgttctt gacgtttctg tagtcgttca tcagctggca
      961 aagcctccca gtctctttcc aaggaaaata actgatcaca ataagctaaa cctttagctc
     1021 ctaaggatga tttatccgct tgcttggggg ggcgcttcaa aaaacttcct tctcacatgc
     1081 gcccaacatc caacaagttc agcttcttcc agttgacgat aagcagacga gcggttcgaa
     1141 gtttaccaag cttagcgtcg ccttgctcct aaaccttgga ctgctatcgc ataggcagaa
     1201 ctagaggact aaagtcctaa gttactgccg caaaatatca caatgcacat agccagaata
     1261 atctcctagg aattcttgta ctactgaacc acttcgacac tgatcatggt ggtaaagcgt
     1321 aatcccttgt ttctctgctt tacctgacaa aaaagtccaa tagtaagtca gctgactatc
     1381 actctctagc accctataag aagtttcatc cgcatgaagt aagggctgag tcaatagtct
     1441 ctctcgcaag aggttataaa ggggctccaa atagtattga ctcgccttga tatgccaatt
     1501 agagatttcc ttacgtgtga ttggtaaacc catcctagcc caatcttctt cttggcgata
     1561 attgggtacc ttcagattaa acttctgatg gatggtgtga gcgataatag aagctgagcc
     1621 aaagttatgc gctaaagggg ctttaggaat aggagatttc acaagcttat ccagatgatt
     1681 atcttttact cgttatggac aatgctatat ggcataaatc aagtacctta aagattccga
     1741 ctaatattgg ctttgcattt attcctccat acacaccaga gatgaacccc attgaacaag
     1801 tgtggaaaga gattcgtaaa cgtggattta agaataaagc cttttaaact ttggaagatg
     1861 tcatgaatca actccaagat gttatacaag gactggagaa ggaggtgata aagtccatcg
     1921 ttaatcggag atggactaga atgctttttg aaagcagatg agtataaaaa gaaagtcctc
     1981 atttcaatag aaatcacgac tttctgatgg atttatagta aaatgaaata agaacaggac
     2041 aaatcgatca ggacagtaaa atcgatttct aacaatgttt tagaagcaga ggtgtactat
     2101 tctagtttca atctactata tttttggagt gatagaaaag cccttcataa gccagtctac
     2161 ttgttctggt gtgagagctt tgacatcctt ttctgtactt agccaaatca atctgccgtt
     2221 ctcaaagcgt ttatatagta gccaaaatcc ttgaccatcc cagtaaagga ctttaaagcg
     2281 gtctttacgt ccaccacaaa agagaaagac ttgaccggag aaaggatcca attcaaagtg
     2341 ggttttaacc agataagcca gtgaatcgat tccttgtctc atatcagttt tcccacacac
     2401 gagatagacc tcccctaggc tagatagatg aattgtcata gagcaacacc ttatccaaaa
     2461 gctgttctac catttcgaga ttgagagatt gaaaaaaact tacttcaagc ttgccaatac
     2521 gaattttaag cagaatatca tttctctttt tagaatca
//
LOCUS       DAVLFB010000154         2517 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLFB010000154 DAVLFB010000000
VERSION     DAVLFB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.154"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..465
                     /locus_tag="VXD58_002048"
     CDS             <1..465
                     /locus_tag="VXD58_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676678.1"
                     /translation="KNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGN
                     VAYYDELIKYKHLRINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPIT
                     KLKKILLPLPPLALQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            525..761
                     /locus_tag="VXD58_002049"
     CDS             525..761
                     /locus_tag="VXD58_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV0676679.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            758..1171
                     /locus_tag="VXD58_002050"
     CDS             758..1171
                     /locus_tag="VXD58_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV0676680.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1289..2254
                     /locus_tag="VXD58_002051"
     CDS             1289..2254
                     /locus_tag="VXD58_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0676681.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            2220..>2517
                     /locus_tag="VXD58_002052"
     CDS             2220..>2517
                     /locus_tag="VXD58_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676682.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKV"
BASE COUNT          921 a          383 c          479 g          734 t
ORIGIN      
        1 aagaatgtta ctaaaaacgg attttcattc gatacaaagc aatttatcac taaaacaaag
       61 gataaattac ttcgaaaagg caaacttgag cgttatgata tagtcttgac aacaagaggt
      121 actgttggaa atgtagcgta ctacgatgaa ttaataaaat ataaacattt acgtataaat
      181 tcaggtatgg taatattacg tcccaagaca ccaaatctaa atcagaaatt tattatccat
      241 gttttaagga ataataatta tagtcgagtg atatcaggaa gtgctcagcc tcagttacca
      301 attacaaaat taaaaaaaat acttctcccc ctccccccac tagccctcca aaatgagttc
      361 gcagactttg tagtccaggt cgacaaatca caattggcaa tccaaaaatc tctggaagaa
      421 cttgaaactt tgaagaaatc tctgatgcag gagtattttg gctgatattc tgccattgta
      481 attacggtaa tgatttgtta taatacttca aaggaggaaa tcagatggta gtaaaaacaa
      541 gaaaacaagg aaattcaatc accattacga ttccaagtga atttaatatt ccaagtggtg
      601 ttaaatacga agcgaaattg ttaccaagtg gtgagattat ctttactcct gaagaattgg
      661 ggcagcaggt ttcttatgta tctgatgatg cctttgactt aaatttagat aaaatatttg
      721 acgaatacga cgatgttttc aaagctttgg tggaaaaatg acaatctatc tgacagaaaa
      781 gcaaattgaa aaaataaatg ctttagcaat tcaacggtat tctccaaatg agaaaattca
      841 aacagttagt tcttctgcct taaatatgat tgtgaactta ccagaacaat ttgtctttgg
      901 gaagtctctt tatccaacaa tttttgataa agcaacgata ctatttgtcc aattgataaa
      961 gaagcatgtt tttgctaatg ctaataaaag aactgctttc ttcgttttgg tcaaattttt
     1021 acaattaaac ggctatcgtt tttctgtaac ggtagaagaa gcagtaaaaa tgtgtgtaac
     1081 catcgcagta gaagctttaa ctgatgaaaa aatgacaagc tactccaaat gggtttctga
     1141 acattctgtt agagaaaagg tcaaaaagta acctagtatg ctggatttga atgagcacaa
     1201 gaaaataaat gaacagacaa tattagaatt ctgtaatgca gaaactgata ttgtctcttt
     1261 ttattgatga ataagaaagt gagaaattat ggaatcaaaa gttacaatta tcatgcaaga
     1321 aatgttacct cttttaaata atgaacaatt actagcgttg agagagagtt tagaacatca
     1381 tctagtagac ggaaaaaagc agcagaagta ttcgaataat aacctgttgc aactatttat
     1441 taccgccaag caggtagagg gctgtagctc aaaaacaatt cgttattacc agaggacgat
     1501 tgaaaacttg tttaatgcta ttaaagagtc tgtgacacaa ctcacaacag atgatttaag
     1561 gagttattta gcaaattacc agtctgaaaa ggattgtagt aaggcaaatt tagacaatat
     1621 taggcgtata ttgtcttctt tttttgcttg gcttgagcaa gaggaatata tcattaaaaa
     1681 tcccattcga cggataaaga aaattaagac tgagcaaaat gtgaaggaaa cttatactga
     1741 tgaacatttg gaaattatgc gtgataactg tgaaaatttg agagatttgg caataataga
     1801 cctactagca tcgacaggta tgcgtgtagg ggagcttgta cagttgaatc gttcagatat
     1861 tgattttgaa aacagagagt gtgttgtctt tggtaaagga aagaaggaga gaccagtata
     1921 ttttgacgct cggacgaaaa ttcatttaag aaattatctt aacgacagaa aagatagtca
     1981 ccctgctctt tttgtaacgc tagttggaaa agcccagaga cttggaattg ctggtgtaga
     2041 gattcgctta agaaagttag gagacaaact cggcatacaa aaggttcacc cacataagtt
     2101 cagaagaact ttagcgacta aggcaattga taaaggtatg cctatcgaac aagtccaaaa
     2161 actgctaggt catagcaaga ttgacacaac cctggcctat gccatggtca atcaaaataa
     2221 tgtcaagcat tcacaccaaa aattcatctc ttaaaagcaa atcccgattt aacgagatgt
     2281 ttggggatcc tttaaataat aacaagaaat ttgcagtaaa aacgggacaa cagtgtttta
     2341 aattttcaag tggaaaattt cttgataaac atgatagagt ttttgaaggt tatcctgcct
     2401 atggtggaaa tggcattgct tggaaaagtc gaaaatattt aattgataat cctactatca
     2461 tcattggtcg tgtaggtgca tattgtggta atgtaagaac tacccacggt aaggtat
//
LOCUS       DAVLFB010000155         2448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLFB010000155 DAVLFB010000000
VERSION     DAVLFB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.155"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..276
                     /locus_tag="VXD58_002053"
     CDS             <1..276
                     /locus_tag="VXD58_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV0676683.1"
                     /translation="FQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGS
                     YLWHNNIDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(359..431)
                     /locus_tag="VXD58_002054"
     tRNA            complement(359..431)
                     /locus_tag="VXD58_002054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Lys,seq:ctt)
     gene            complement(696..1967)
                     /locus_tag="VXD58_002055"
     CDS             complement(696..1967)
                     /locus_tag="VXD58_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV0676684.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            2237..2437
                     /locus_tag="VXD58_002056"
     CDS             2237..2437
                     /locus_tag="VXD58_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676685.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQ"
BASE COUNT          714 a          456 c          568 g          710 t
ORIGIN      
        1 tttcagcatt ttggctatgt ctacgatgtt ccagatattg caacagtcaa taaagctata
       61 aaagtaatgt tgaaggaatt gcaaattttt ccattgatta ctaccaaagg agctagacac
      121 acttatggta gttatttatg gcataataat attgatttgg gagttatagc gaaaatttta
      181 ggacataaag atatctccat gttgattgac gtttatgggc ataccttgga ggaaaaaatt
      241 agtgaagagt ttatggctgt gaaaagtttg ttgtaaaaac acggcaaatt gcacgacaat
      301 ttttaaaagg aaggcaaaaa aagccctata ttataaggct tttcctgttg tatttatatg
      361 ccccctacag ggatcgaacc tgtgacccac ggattaagag tccgctgctc tgccagctga
      421 gctaaggagg caaagaaaaa agctgtattg gtgccgaaac ttcacggttt gtattgaacc
      481 cgcgcaatta agcaggtggg caactcgctc taactgaagc tgtttccgtg tgagacggcc
      541 tacatgctgt tagaagactt ttgtttccct aataatacaa aaaatagtcg gtcaacactt
      601 aagtgtgaag tcgtacacca cagcgtttct atacttatat gataccactt tttcaaaaaa
      661 aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga tttttcgcaa acggtcgata
      721 gcttcctttc tttgagccaa agcccgttca tatttaccag tatcttctgc ttggaaatag
      781 tgatgattac gaattttttc tggcagatag tcttgcttga cccaatttcc aggatagttg
      841 tgtggataga gatagtcttg ggcattcccc agttccttgc ttccactgta gtgcccatca
      901 cgtaggtgtc gcggaatagg caagtgccct gatgttttga ggtcagcaag tgccttatcc
      961 atagctacat aggctgagtt ggattttgga gaaagggcca aatcaatcac gacattggca
     1021 atgagaatgc gggcttctgg gaacccaatc ttctgggcag catccagagc agtcacggta
     1081 tgaatctggg cctcaggatt ggctaagccg atatcttcat aggcgataac agtcaagcga
     1141 cgagcgagac taggcagatc cccagcctca atcaagcggg cagtatagtg gagactggca
     1201 tccacatctg agccacgaat agacttttgc agggcagata gaacatcata gtgaccgtct
     1261 ccatccttgt ccatagtgat gtagcttctc tgaagactat tttccatgat gtctaaggtg
     1321 atgtggcgaa tgccctcgtc attctcaggg gtagagagaa cagctaagtc cagtgagtta
     1381 aaggcagagc gaaggtctcc gtttgtagag gttgcgatga aatccagcgc atcctcatct
     1441 agttctattg gaaaatcaaa accacgttca gggttactta gagctatctg cagggcctct
     1501 ttgacgtctt ggttagacag aggttccaac tcgaaaattt gaactcggct acgaatggca
     1561 ggagtgacag agaagaaagg attttcagtc gttgctccaa tcatgatgac cagtccactt
     1621 tccaagagag gcaagaggaa gtcttgcttg gtcttatcta gtcgatgaat ttcgtctagc
     1681 aataggacga gaccaccaga aaatttagct tcttccgaga tttcttgcag tcgcttttta
     1741 ctatcaactg tcgcattgaa agttcgaaag gcatacttgg tcgttccagc gatggcagag
     1801 gcaatactgg ttttgccgat tccaggaggg ccatatagaa tcatggagga caggcggttg
     1861 gcttccacca tgcggcggat gatttttcca ggtccgacca gatgctcctg accgatgacc
     1921 tggtcgatgg ttttagggcg catgcgaagc gcgagattgt ctggcatagc agtcctttct
     1981 agcgtggatt tgagtagaat gactcatcta ctatatctta ctatgtccat tctagcctat
     2041 tagttgtgat atttcaatgg ttttgtaata atcagggagt gagatagaat cttttttact
     2101 atttaattta atgaggagag gtatcagcat gagagatttg attttcctta aagctcaaaa
     2161 taggatagtt gagcctaaag aagtggaagt gctgacttga gaaataatat tgctgaatgt
     2221 cattgtatga actataatga aaagagaggt gataagcaat ggaaatgatg gaccaagtca
     2281 ggaaattttg atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc
     2341 agggaaaaga aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga
     2401 aaacattaaa tctaaacttg attttgctta ccaataagtt tattctaa
//
LOCUS       DAVLFB010000156         2395 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLFB010000156 DAVLFB010000000
VERSION     DAVLFB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.156"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            129..1622
                     /locus_tag="VXD58_002057"
     CDS             129..1622
                     /locus_tag="VXD58_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV0676686.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2177
                     /locus_tag="VXD58_002058"
     CDS             1635..2177
                     /locus_tag="VXD58_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676687.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQLDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRYSEEVVA"
     gene            2174..>2395
                     /locus_tag="VXD58_002059"
     CDS             2174..>2395
                     /locus_tag="VXD58_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV0676688.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQW"
BASE COUNT          835 a          355 c          487 g          718 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caattagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatattccg
     2161 aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg gctactttta tcaatggcta
     2221 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa tacagaatct
     2281 gactaaaact tcaaaaggga taaattatta ttcgggtacg attgataaaa aatacattgt
     2341 tgaagctggt gatattctta tttcgtggtc gggtacatta ggggtttttc agtgg
//
LOCUS       DAVLFB010000157         2277 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLFB010000157 DAVLFB010000000
VERSION     DAVLFB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.157"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(160..2142)
                     /locus_tag="VXD58_002060"
     CDS             complement(160..2142)
                     /locus_tag="VXD58_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV0676689.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKVLDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT          639 a          472 c          402 g          764 t
ORIGIN      
        1 caataggaaa caagtaaaat taacaaaaat tttctctatt ctgacaaaaa gaggctccaa
       61 cttttggggg tcagtacatt tagaatcttt ctttacaaat gtaaaaaagg attcagcatt
      121 caagagaaat actaaatcct ttcttatatt ttgcaacagt tatttcacat gttttgcgag
      181 atcttcttga gcctttttat tagactcttc tttttctttc atccattttt cctgagcttt
      241 ttggtattca tctacagtga ctgccttgtc ttgaagttcc aagtatttat ataagattgg
      301 ttcacttgta cctttatttc ctgacaatgc aaatggtatt gtaaatggta ccatcttaga
      361 caagattggg cgccctgtac gagatgtagt tggaataatc aaagcactat ctgtcaacca
      421 agcttgggct gcagcgtatt tatcatagcg tttagcaaca tctgtagcct catcaccagc
      481 ctcagtaacc aatttttcgt agtcatatag acctactttt ttagcagcta cattatcttc
      541 ccctgagtca aaccctaaat atgttttagt actttctcct acagatggtt tgatgatatc
      601 aaggtaagtt gatggatcgg caaagtctgg accccaaccg acattatctg ataaatccca
      661 gtcttcgcca gcagcatttt cagcaaaata tgtaatattg tttacttcgt ctttttgtag
      721 ttgttggata tcaataatga cattatcagc tcctaaagtt gcttccaagg attgtttcat
      781 agattggacg cgctgaactt ttgtagttgc tgtctggtca actggcatat ccaaatgaat
      841 tgggaatgtc acaccttctg cttgtaaggc tgatttagct ttagcaaatt cagccttggc
      901 tttttctgga ttgtaaagac catcctgaga atctgcaaga ttaacatcct tccattcatc
      961 cccataagtg accaatttct ctttgaccat atcgccaaag tttttaccat ctgcttgaac
     1021 aaatgttggt ggcacaaaga gattacgcaa gattttactt gctccagttt gtccattcaa
     1081 ctgagaggca taggctgtac gatcaaaacc aaaggcaata gcctgacgga aatccttgtt
     1141 taagagagcc tttttagtcg atgccttttg ttcgtcgctg gtcttagatg tgtatttata
     1201 ggactgacgg tcaatatttg taccaactag atacgtaata gagtcttgtt gagtatagac
     1261 aatattgtcc ttcatactct tctcaagctc tgcgaaactt gcacttgttg gatagagacg
     1321 agctgctgta aggctaccat ctttaaagtt ttctgcaggt ttgctggtat cttgaccatc
     1381 ccagaatgac aatttaactt tgtcaatatg cacattgtcc ttatcccagt agttcggatt
     1441 tttcgcaaat tcaacagaag atttggtcac aatggatttc aacaaataag gaccgttata
     1501 caagagacta cttggatccg tagctttggc aaaatcatcc ccttttgaat tcaaaaactc
     1561 ttcattaact ggcgcaagca cacccatggt tgtcttagaa ttccagaagc tttctggttt
     1621 gttcaaagtg tactgaactg tctgttcatc cagaacctta attcctactt gtgagaaatc
     1681 tttgatttcc ccttttacat aggcatccaa ccctttgatt gattcttgaa caaggtaaag
     1741 agcatctgat tttttatcag cagcatattt tagtcctgtt acaaagtctt gagctttgac
     1801 tgccgcgtat tcttcccctt cagaagtata ccattttgca tccttacgga tagtataagt
     1861 gtaagtcaat ccatccttgg atacagacca atcctcagcc atagacggca caaagttccc
     1921 gtagcgatca ttttctagca aaccatcaac cacgttactg gtaatatttg ctgtcgcagc
     1981 cttagcagtt gtcaaatagt tgaggttatc agggtctgtc tcataaatgt atgagaatgt
     2041 cttctcacct ttagtgcttg aacctgatcc agagcatgca gctaaagtag tcgccgccaa
     2101 taatgtcacg cccgcaaggg caaatagttt tgaacttttc atcattttct cctttaaaac
     2161 tttctctcca ttataaactc ttttcagaaa gatgtcaaca gaattttcag aatttttgtg
     2221 ttaatttggt atattctgaa aagacaaaaa aagagaagac agtgattgtc ctctcca
//
LOCUS       DAVLFB010000158         2190 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLFB010000158 DAVLFB010000000
VERSION     DAVLFB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.158"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            102..1547
                     /gene="cps4A"
                     /locus_tag="VXD58_002061"
     CDS             102..1547
                     /gene="cps4A"
                     /locus_tag="VXD58_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV0676690.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHRKFLFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTDRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1549..>2190
                     /locus_tag="VXD58_002062"
     CDS             1549..>2190
                     /locus_tag="VXD58_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV0676691.1"
                     /translation="MIDIHSHIVFDVDDGPKSIEESKALLREAYNQGVRMIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYREIHAGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVLKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAY"
BASE COUNT          698 a          355 c          461 g          676 t
ORIGIN      
        1 acattcgaat tacacaagat gcagatagtg aaaataaagg tgtagacatt accgtaaaaa
       61 agtgatataa ttgtatgatg ttcaatgtat aggtgttaat catgagtaga cgttttaaaa
      121 aatcaggttc acagaaagtg aagcgaagtg ttaatatcgt tttgctgact atttatttat
      181 tgttagtttg ttttttattg ttcttaatct ttaagtacaa tatccttgct tttagatatc
      241 ttaatctagt ggtaactgcg ttagtcctac tagttgcctt agtagggcta ctcttgatta
      301 tctataaaaa agctgaaaag tttactattt ttctgttggt gttctctatc cttgtcagct
      361 ctgtgtcgct ttttgcagta cagcagtttg ttggactgac caatcgttta aatgcgactt
      421 cgaattactc agaatattca atcagtgtcg ctgttttagc agatagtgag atcgaaaatg
      481 ttacgcaact gacgagtgtg acagcaccga ctgggactga taatgaaaat attcaaaaac
      541 tactagctga tatcaagtca agtcagaata ccgatttgac ggtcaaccag agttcgtctt
      601 acttggcagc ttacaagagt ttgattgcag gggagactaa ggccattgtc ctaaatagtg
      661 tctttgaaaa tatcatcgag tcagagtatc cagactacgc atcgaagata aaaaagattt
      721 ataccaaggg attcactaaa aaagtagaag ctcctaagac gtctaagaat cagtctttca
      781 atatctatgt tagtggaatt gacacctatg gtcctattag ttcggtgtcg cgatcagatg
      841 tcaatatcct gatgactgtc aatcgagata ccaagaaaat cctcttgacc acaacgccac
      901 gtgatgccta tgtaccaatc gcagatggtg gaaataatca aaaagataaa ttaacccatg
      961 cgggcattta tggagttgat tcgtccattc ataccctaga gaacctttat ggtgtagata
     1021 ttaattatta tgttcgtttg aattttacct catttttgaa attgattgac ttattgggag
     1081 gggtagatgt tcataatgat caagagtttt cagctctaca taggaagttc cttttcccag
     1141 tagggaatgt ccatctagac tctgagcagg ctctaggttt tgtacgtgaa cgctactcac
     1201 tagccgatgg agaccgtgac cgtggtcgca accaacaaaa ggtgattgtg gctattcttc
     1261 aaaaattaac gtcaaccgaa gcactgaaaa attatagtac gatcattaat agcttgcaag
     1321 attctatcca aacaaatatg ccacttgaga ctatgataaa tttggtcaat gctcagttag
     1381 aaagtggtgg aacttacaaa gtgaattctc aagatttaaa agggacagat cggatggatc
     1441 ttccttctta tgcaatgcct gacagtaacc tctatgtgat ggaaatagat gatagtagtt
     1501 tagctgtagt taaagcagct atacaggatg tgatggaggg tagatgaaat gatagacatc
     1561 cattcgcata tcgtttttga tgtagatgac ggtcccaagt caatagagga aagcaaggca
     1621 cttttaagag aagcttataa tcaaggagtt cgaatgattg tgtctacttc gcatcgtcga
     1681 aaagggatgt ttgaaactcc ggaagagaag atagcagaaa actttcttca ggttcgggaa
     1741 attgcaaaag aagtagcaga tgatttagtc atagcttatg gcgcagagat atactatact
     1801 ctggatgctc tagaaaagct agaaaaaaaa gaaattccta cccttaatga tagtcgttat
     1861 gctttgattg agtttagcat gcatacttcc tatcgtgaga ttcatgcggg attgagcaat
     1921 attttgatgt tgggaatcac gccagtaatt gctcatattg aacgttatga tgccttagag
     1981 aataacgaaa aacgtgttcg tgaactgatt gatatggggt gctatactca gataaatagt
     2041 tatcatgttt taaaacctaa gttctttggt gaaaaatata aattcatgaa aaagagagct
     2101 cggtattttt tggaacgtga tttagttcat gtagttgcaa gtgacatgca caatttagac
     2161 agtagacctc catatatgca acaggcatat
//
LOCUS       DAVLFB010000159         2182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLFB010000159 DAVLFB010000000
VERSION     DAVLFB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.159"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..868)
                     /locus_tag="VXD58_002063"
     CDS             complement(<1..868)
                     /locus_tag="VXD58_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV0676692.1"
                     /translation="MKIIGIDIGGTTIKADLYDDFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYVLNYSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTSEIEKRF
                     GLSTFVENDVNCAALGELWEGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLSSGDKVAKETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNVVAATL
                     GNEAGRIGAVKNF"
     gene            complement(1025..2134)
                     /locus_tag="VXD58_002064"
     CDS             complement(1025..2134)
                     /locus_tag="VXD58_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV0676693.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
BASE COUNT          664 a          475 c          302 g          741 t
ORIGIN      
        1 agaaattttt tacagctcct atacgaccag cttcatttcc taatgtagct gccacaacat
       61 ttttaggcaa aaacctatta tccattgcat ttttagctaa agaactttga atttcaggta
      121 acaaaatatc ctttttagcc agaatcccac ctcctaatat gagaatttct ggattaagta
      181 gataagaaat agttaataaa ccttttgtta gattttctac aaaaatttca aaagtttctt
      241 tagcgacttt atctccagaa cttaaatcag taaagaaagt acgtccagtt tgattagttt
      301 tcaagctctt cttttgatat aaatgaatca atgcggttgt tgaggctttg ctttgccaat
      361 ccgaattacc tacaggaata tagcctactt caccagcagt atagttaaat ccgttaacaa
      421 tttgtccatt gacaataata ctgcctccta tacctgttcc aatagtaacc attactacat
      481 ttttcttatc tttggcttgt ccctcccaca attcacctaa tgcagcacaa ttaacatcat
      541 tttcaacaaa agtagacaac ccaaaacgtt tttctatttc ggaagtaaag tttactccga
      601 tataccctgg tattgtatag cctgcataga tgatttctcc tgtattagca ttaacaactc
      661 cagcagtgga aatcccaaca ccatcaattg aataatttaa aacatactca ccaattaaat
      721 cacagacctg atttaatatc tggttcgttc ccaaatcata gtcaataatt gtttctatct
      781 ctttgaactg atttaaactc gttccaaaat catcgtataa atctgcctta attgttgttc
      841 cgccaatatc gatccctata atcttcattt catcactccc acatttttga tctcattact
      901 ttttgataaa aaatgggact gggcattatc ccagtcccat ctctatttct ttattcaatg
      961 agttgtatca cagaacttta gaaattatat aatgacaaga atttactatt ctgttttaca
     1021 atagttaagc atttgtgatt tctgaaagac ttacttttcg atcttcttta actgataaag
     1081 tacatgcatc agcggtagcg attgattcta aagcagctac accattgaga agtttttcat
     1141 attcttctgt aatttctcca cctttaatga tgtcatgtag atattccatt tctttatcaa
     1201 tacatgtttg taaccataat gggcaacgta ctcctggttt accgtacgca attgctccat
     1261 ccataccacg accggtatag atagctgtac ggtcatcatc ttcctcttga gtttcatgta
     1321 ctaagaagtg tgattctcct tcacctttaa cacgaagagt acctccagta ttgaacaagt
     1381 caagtttgat agctccttca gttccttgaa tcaagacgta gtgttcaccc caacggaaag
     1441 cattaccata ttccaaaaca gcataacgat catcagagta ttctaggttt acaatgagca
     1501 tatcatcttc atcaccaaag ttttcacctt tatgatatac attgcctcct accattgtcg
     1561 ctttttcagg aagtcctccc atgatgaact gaatgcaatc taattcatga atatggtggt
     1621 acaaatgtcc tccagattga gaacgaagtt tcttccatga tacagttggt tgttgttctt
     1681 cccaacctgt acgagcggca tggcaataaa gaactttacc gattttacct tgagtaatca
     1741 attctttagc atggtgtaca ccattaaaga agttcatgat gtgaccagcc ataaagataa
     1801 cattattttc tttacatgcg tcaaccatgg ctttacaatc ttcataagac aatgcaattg
     1861 gcttttcaca aaatacgtgt ttgccatgtt gagcagcttt cacaacaggt tcacggtgaa
     1921 ggtagctagg tgaagctacg atcacacaat caatatcttc acgtgctaca agttcatcta
     1981 aacttgcaca aacatctgat cccaactctt gagcaacttc ttctccatga tttgggtcaa
     2041 ataccgcaac cacttttgca tcttcaattt tgttcattga gcgagccaaa tcagctccaa
     2101 aatatccagc tccaacaata ccgtaattta ccataatttt tatcctcttt ctatatattt
     2161 ttaaatttca gctttaattc tt
//
LOCUS       DAVLFB010000160         2166 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLFB010000160 DAVLFB010000000
VERSION     DAVLFB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.160"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..1149
                     /locus_tag="VXD58_002065"
                     /pseudo
     CDS             1..1149
                     /locus_tag="VXD58_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1264..2067)
                     /locus_tag="VXD58_002066"
     CDS             complement(1264..2067)
                     /locus_tag="VXD58_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV0676694.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          689 a          451 c          364 g          662 t
ORIGIN      
        1 aattccttac cttgaaacga ctggtatgcc tactagaatt ctccttagaa aacgtcgttt
       61 taagtgctat cattgcttga aaatgatggt cgctgaaact tctatcgtca agaaaaatca
      121 ccaaattcct cgtattatca accaaaagat tgcgcaaaag ttaattgaaa agatttctat
      181 gaccgatatt gcccatcagc tggccatttc aacttcaact gtcattcgaa agctcaatga
      241 cttccacttt gagtgtaatt ttagaaatct gcctaagatt atgtcttggg acgttgaaac
      301 agtccgggga gtgactgttt caatcgggag atggagatga gctttattgc gcaagatttt
      361 gaaaagctcg atatcatcac tgttcttgag ggtagaacac aagctgtcat ccgagatcac
      421 tttcttaaat atgatagagc cgtccgatgt cgcgtcaaaa ttattactat ggatatgttt
      481 agtccttact atgacttagc taaacagctt cgctttcaaa tttctaggct caggctgaaa
      541 cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc tttcatattg
      601 tacaacatct tagccgtgct atgagtcgtg tgcgtgtcca aatcatgaat cagtttcatc
      661 gaaaatccca tgaatacaag gctatcaagc gctactggaa actcattcaa caggatagcc
      721 gtaaactcag cgataaacat ttttatcgcc ctacttttcg tatgcattta accaataaag
      781 agattttaga caagcttttg agctattcac aagacttgaa acatcactat cagctctatc
      841 aactcttgct gtttcacttt cagaataagg aaccggagaa atttttcgaa cttatcgagg
      901 acaatcttaa gcaggttcat cctatttttc agactgtctt taaaaccttc ctcaaagata
      961 aagaaaaggt tatcaacgcc cttcaactac actattctaa tgccaaactg gaagcgacca
     1021 ataatctcat caaacttatc aagcgcaatg cctttggttt tcgaaacttt gaaaacttca
     1081 aaaaaacgga tttttatcgc tctgaacatc aaaaaagaaa ggacgaaatt tgtcctttct
     1141 cgaacttagc ttttcttcaa cccactacaa ttgacaaaga gccaaacttt gatgatatac
     1201 gttttcttgt gagaatattt acttcatttt ttcctaaaat tcaatgttta ctcagtattt
     1261 ggattatgaa aaatcgaggt ctaaatctag atacattttt tctgaagaca aatcattttg
     1321 accaccgagc aagagatttt caaaaaaagc tgttaaaaac tcaggacgtc gctgtaaaat
     1381 ctttgcatta tctaatacca aggcatcacg aaaatacttg gaatgttgct gaaatggtat
     1441 attatcaata tcaaaaccaa actcacgaag atactgaatc aaaaagaccg ttactgtccg
     1501 agtgtttcct tcgcgaaatg gatgaatctg ccagattcct gaaataaaat gctggatttg
     1561 tttaaccaca tccgcctgag ttagtgtcgc atatgcaact tgtttttcct gattaaaatc
     1621 ataatctaag gtcatttgaa tcatggagta atcagagtac acaacacttt caccattcaa
     1681 aacaggttca ttctttgtga tattggtctg acgaaattgc cccaccggaa tagagggttc
     1741 aaatatatct tgaaacaact ccttatgaat agcaagtaag gtcgcaggac taaagctaaa
     1801 gcctcttcga gacaatagtt ctacaatacg tagagaaacc aagtctgcct cttccgtact
     1861 tgcatcaata gtatggtgat aagccgttgc atcctcataa acctgctcat aagtcagttc
     1921 tccccgggac tgtttctcag ccaaagattc catatacgct gatggcacta gattgtcaac
     1981 tttctgcaga ccaaaaccta tccgccataa atcacgcttc gcttcataag acaagtttgg
     2041 attgtcaatg ttgtaagttg gttgcataga aatatccttt caattgtttt tcttcattac
     2101 cagtaaatca cgaaattggt tggaaactgg cttaaactat aaaaagtcct cacaataggt
     2161 tctatg
//
LOCUS       DAVLFB010000161         1981 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLFB010000161 DAVLFB010000000
VERSION     DAVLFB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.161"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..1000
                     /locus_tag="VXD58_002067"
     CDS             <1..1000
                     /locus_tag="VXD58_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV0676695.1"
                     /translation="ILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDK
                     KPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFD
                     DLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKID
                     SKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTS
                     KDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEAL
                     QKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            990..>1981
                     /locus_tag="VXD58_002068"
     CDS             990..>1981
                     /locus_tag="VXD58_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV0676696.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKN"
BASE COUNT          692 a          286 c          356 g          647 t
ORIGIN      
        1 tattttagct atgtgtatag tttttagcgc ttgttcttct aattctgtta aaaatgaaga
       61 aaatacttct aaagagcatg cgcctgataa aatagtttta gatcatgctt tcggtcaaac
      121 tatattagat aaaaaacctg aaagagttgc aactattgct tggggaaatc atgatgtagc
      181 attagcttta ggaatagttc ctgttggatt ttcaaaagca aattacggtg taagtgctga
      241 taaaggagtt ttaccatgga cagaagaaaa aatcaaagaa ctaaatggta aagctaacct
      301 atttgacgat ttggatggac ttaactttga agcaatatca aattctaaac cagatgttat
      361 cttagcaggt tattctggta taactaaaga agattatgac actctatcaa aaattgctcc
      421 tgtagcagca tacaaatcta aaccttggca aactttatgg agagatatga ttaaaattga
      481 ttcaaaagcc ttaggtatgg aaaaagaagg tgatgagtta atcaaaaata ctgaagctcg
      541 tatatccaaa gaattagaaa aacatccaga aatcaaagga aaaatcaaag gaaaaaaagt
      601 attatttact atgattaatg ctgcagatac atcaaaattc tggatttata ctagcaaaga
      661 tccaagagca aattatttaa cagatttagg tctagttttc cctgaatcat taaaagaatt
      721 tgagagtgaa gatagttttg caaaggaaat ttctgcagaa gaagcaaata agataaatga
      781 tgctgatgta atcataactt atggtgatga taaaactctt gaagctttac aaaaagatcc
      841 tcttttaggt aaaataaatg caattaaaaa tggtgccgtt gctgtaattc cagataatac
      901 accgttagca gcctcatgca ctccaacacc actttcaata aactatacta ttgaagaata
      961 cctaaatctt ttaggaaatg catgcaaaaa tgcgaaataa aaaacaaata aacctaggca
     1021 taatttttgt aatctgccta ggtcttctta ttacaatatt tttgtcatta aagcttggaa
     1081 caaaagaaat taatatcaga gattttttag cagcttttgg aatgggcaat acaaatgatg
     1141 attttattaa atcaattata tataaaagaa tacctagaac tatttttgca attttagcag
     1201 gttctagtct tgccataagc ggtgtattga tgcaatcagt tactagaaac ccaatagctg
     1261 atccaggtat actcggtata aacacaggag caagtcttag tgtagtaatt ggtctttctt
     1321 ttttaggaat ttcatcaagc ataagccata taagttttgc aatcattggt ggcttagtaa
     1381 gtgcaatttt tgtatacgcg attgctgtaa gcggaaaagc aggccttacc cctataaaac
     1441 ttgccttatc aggaacttgt gttagtatgg ctttaagcag ttttgtaagt tttttaattt
     1501 taccgaataa taacgtctta gacaaattta gattttggca aataggtagc cttggagcag
     1561 ctacattatc ttctatatct acactactac cttttataat tttaggtcac ttgatagcta
     1621 tatttatttc atcagattta aacgctttag ctatgggtga tgaaatggct gttggtcttg
     1681 gagttaatgt taataggata agatcacttg caataattgc aagtgtgctt ttatgttcaa
     1741 gtattactgc aattggtgga cctattggct tcgtaggtct tatagttcct cacttttgtg
     1801 gcttatttat aagcaaagat atacgcacaa tgaccatttc ttcatctttt ataggtgcag
     1861 agctcttgct tatatgtgat ataatcggcc gtatgttagg taaaccaggt gaaattgaag
     1921 tagggataat tactgcaata atcgggggtc cagtacttat ttatgtaact atgaaaaata
     1981 g
//
LOCUS       DAVLFB010000162         1945 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLFB010000162 DAVLFB010000000
VERSION     DAVLFB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.162"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..200)
                     /locus_tag="VXD58_002069"
     CDS             complement(<1..200)
                     /locus_tag="VXD58_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676697.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVD"
     gene            complement(258..971)
                     /locus_tag="VXD58_002070"
     CDS             complement(258..971)
                     /locus_tag="VXD58_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV0676698.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1429..1809)
                     /locus_tag="VXD58_002071"
                     /pseudo
     CDS             complement(1429..1809)
                     /locus_tag="VXD58_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
BASE COUNT          641 a          388 c          306 g          610 t
ORIGIN      
        1 ttatctactt gtgaatccct tctcccaata taatcccctt ctttgactaa tgatggagat
       61 ttccaagtaa aaattaaatc gccagagaaa tttttcttat caaaaaactt aaaaagagat
      121 tgataaatac tatttgctaa ttgacaagtg tcagatgtaa attcataatt aacagcttca
      181 ttaaaatcaa cttcagacat ttcaatgtac tcttcaatag taatattagc ttttttgtag
      241 tcattagctc gtctccttta aaatgtgatc ttttcaagaa tatttttatc taaacaatcc
      301 agcaagtctt ggtaagaata gacttcgtaa gtcggctggg cttgtgtgtg attttcgagg
      361 tgatgaggat tataccagat agtgtcaatc cccgcattat tgccaccttg aatgtcggcg
      421 gttagagaat ctccaatcat cagcgtcttt tctttactaa atccagcaat ttgctggcca
      481 atcttttcat aaaaaagagc atccggcttt tgagtttgca actgttctga gataaagact
      541 tgattgaaat aaggtgctag accagattga gccaaacgtc ctgtctgaat ggcagtaatg
      601 ccatttgtcg cagcatacaa gtcataatca cgctcaatga ggctgtccaa gagatcatga
      661 gcgcccgata gtgtttgtcc ctgctgagcg agataaaatt ggtaacgctg ggcaagaaaa
      721 ctaccgtctt tttcctgtcc aaaatgagaa aataaacgag aaaagcgcgt gttaaccagc
      781 tcttgtttac tgattttctt cagctccaag tctttccaga gagccttgtt cataggaacg
      841 taataatctt tataagcctg aatatccgca actccttctt cttttagaag ttgggtcaaa
      901 gccacatcct cagcagcatc aaaatcaaga agagtgtggt cgaggtcgaa gagtagaaat
      961 ttgtagaaca atttgaggtt ttcctttctg aaaattcatt aagaacatta tatcataaag
     1021 cacctcatac aattaactaa tttaatcact taaaaaaatt cgaacacttt ctatacaact
     1081 gacagctcaa atctttcaga atagaacaat actaactatc gagcaccccg tattcataaa
     1141 tatatgtgta attctagacc tagaattcct ataaactaaa tgctttcata ctcttccaag
     1201 taattgattg ccttaaattt taatttttga aggtttctaa agctagaata gccccatcac
     1261 aatcagtttt gattgattca caatttagaa acactatagt ttcactcctg ttaaaataaa
     1321 aaggaactgc ataaagcaat ccctttctga ttttgaaatc atttacttaa cattttatag
     1381 ttgagataat caatagctta cctataaaaa gagttatagt aaaattcctt atttattgat
     1441 ttcaagctcc gctaactgtg tttgaataac tgacagttct gcaccagcct gaagaagagc
     1501 agctgcatta taggcacctt ctacaattgg aaccctgttg atgatgatac ttttatcact
     1561 gaaatcagtc accattttta agttcatttt agcagaacct aggtcaaaaa aggcaagtaa
     1621 agtatctgct gtattttcgg aaacaaccct atctacttaa tcaaaactcg ttccaattcc
     1681 tccgccctcg gttcctccta cataagtaat cggaacatct ttagctactt tactaatcag
     1741 ttcaacaaca ccttctgcaa tgtgtttgga atgtgaaacg ataacaagac caataccaat
     1801 actttccatc aaaccacccc agtttctaaa atagcagtaa agagtaatcc tgatgagaat
     1861 gatccaggat caatatgtcc aagaaaccat atgctcctaa gacaagagcc aacagactgg
     1921 tcatcaataa tagtattgtt ctttt
//
LOCUS       DAVLFB010000163         1900 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLFB010000163 DAVLFB010000000
VERSION     DAVLFB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.163"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(332..1225)
                     /locus_tag="VXD58_002072"
     CDS             complement(332..1225)
                     /locus_tag="VXD58_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676699.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          640 a          380 c          207 g          673 t
ORIGIN      
        1 atacgattac tacctccttt ttagtttaca cttcctacat cataaataaa catactcaac
       61 cctcacaatc tccaaataac tcttctatat cattgaaaat tgagtttcat cctctatact
      121 aatgtaggaa acctattaaa acattccttt aatccaatat aagaatacct tgttccatta
      181 atctctaccc gatattatag atagaaccag actcctatac ttaaaaggac aaataacgtt
      241 ccaaccataa taaacctcct ttctttttga gaacgttttc tttataggtt tagtatagca
      301 aaaacatgaa aaaaacaacc tgacttagaa gtcaggttgt tgggaaatct atctgttttt
      361 ctgctaaaat tctctgaact aatacctgat cgtctagcaa ttctgctaga gaaatcgcct
      421 tttcatagaa actttctgcc tctaaaatat tcttgtctac aaataaagca tattttgcct
      481 ttagcaaaaa tagatgaggt cggtaaaaaa catgtcttgt tttagcaata attttctcaa
      541 atgtttgaat atatatcaga ctatcttctg tctgaatttg gataaatagg ctcagaagaa
      601 ctttttccat ttgaactagg ctagccttat cttcatattc tgaacatagc aatacttttt
      661 gagctaactc ctcaaaatgt tctttatctt ctaatcccat agcacaacaa aagaaataca
      721 attcaataat caacaaatca tttacagaaa aatatttctt ttttaataac tgctggaaat
      781 attcttccac taaccctaaa ccgtaggtca catctgaact accataaata tcaaaacgag
      841 cttgtgtcac atccacaatt aactgctctt cctcaggcaa attctcataa tagttttcat
      901 atatttcctc aagtatagct tcttttcgtt cgattcttcc cttatcagcg taggtttgag
      961 aatgaatcag ctcatctttg agttttagga atccttttgg aatctctata accttaacat
     1021 ccacaatatt ctgtaatgat actccaagtt tatccgcaat caataataat ttaggaagtg
     1081 ttgctaaagc ttgcccattt tcaatacgtg ctagctgacg aatcgtcaac tctgattcat
     1141 caccacaaat atctatctga gtttttccac aagatcgcct aatagttttg attttataac
     1201 caatttcacg tttcaactca tccatagtaa tactccttaa gttgattaca aagatttctt
     1261 tttcttccta attcttttgt caatcatata taaaacgata agcgaaaaca gcggtagaac
     1321 aatataactc ttttcactca ttatcagctg aaatcccagg gttgctagta tcttaaagat
     1381 gaaaatattc atcaaaaata gaaaaacaaa tactaaaaaa tattttaaaa ccttcttcat
     1441 ttcttttctt caactttcct aatttctaca cctattttat cgaaaatata tataaaactc
     1501 caaataaaca cttcgactgc actttttttg gcaaaaaata gacagaacaa acatcctatc
     1561 tactaaaaat atgatatgaa aggaaattga ttttttactg tcaccctagg caagcttgaa
     1621 acaaataaat cattgatagt aacacttacg acatcctatt tattacttca atttctaatg
     1681 tctctgctat ttctaagcta cctctggtat gatctattcc tagtcagaat agataacacc
     1741 gttctaaatt acgattcata gttactcctt ttttgactcg ttactataag actcctcttt
     1801 acatgcgcaa aataattttg attcagattc atcatttata gttttgatta ttttttaaca
     1861 gatttttctg ttttcttctc cgaagacttt aaaaattttt
//
LOCUS       DAVLFB010000164         1812 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLFB010000164 DAVLFB010000000
VERSION     DAVLFB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.164"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(134..802)
                     /locus_tag="VXD58_002073"
     CDS             complement(134..802)
                     /locus_tag="VXD58_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV0676700.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFANKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLIIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(913..1704)
                     /locus_tag="VXD58_002074"
     CDS             complement(913..1704)
                     /locus_tag="VXD58_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV0676701.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     AKEIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDDLKEAIQSFQY"
BASE COUNT          607 a          388 c          323 g          494 t
ORIGIN      
        1 agatgaaact gacgaagtca gtaaccatac ctacggcaag gtgaagctga cgtggtttga
       61 agagattttc gaagagtatc atacgaaaaa gccctgttcc tcaagatgag gagcaaggct
      121 ttttgagttt caattattct tctgttccaa ggaaattttt tgcaacaagg gctgcaagaa
      181 ctccaccagc gattggtgca aggatgaaaa tccaaacttg ttgaagggct gcgccgccta
      241 ccaagacagc tggtgccaag ctacgagctg ggtttactga aagtccagta atcttcagtc
      301 cgacaagaat catcgccatc aatgacaaac cgattaccaa accagcaatc gcgccattgc
      361 ccttactttc tgaagtcaca gtcatgataa tcaagacaaa caagaaagtt gcgatgactt
      421 caaacaagaa accaccaaag acagtgacac cgtttgccaa ggcattttca ccaagactag
      481 cagttgacat acctgaatta cccaagagga agaagactgc gccagaagcg atgaaagctc
      541 caacaacctg accaaggatg tagtttacaa gttctgaaga tgacaaacgt ttgtttgcaa
      601 acatagcaat cgaaacagcc gggttcaagt gagcacctga aacagttccg attgagtagg
      661 ctgccaccac gattgccaaa ccaaaggcaa aggcgattcc aaggtgacca aggccatcaa
      721 gaccatttcc aaaaacaaca gctcctgtcc caacgaacac aagcatgaac gtaccgatta
      781 actcagcaac aaattttttc attttctttc tccttttttc aaaaactaga tactagtcta
      841 tcaaaagtag gaaagggttt caagaaaatt gattggaaat tttttgaaaa tcatagaact
      901 attagctaat ccctagtatt gaaaagactg gatagcttct ttcaggtcat cttgtaaact
      961 atttctctgg tcaagttgga catagacttc caccagacag gatctaaagt tggaaaattt
     1021 gtaaaaatcc tccctttctt ctatcggaaa atcaacagtt tttatccaag aagctacttg
     1081 ttcttgctcc aacttccctt gtaaaatagg ttcatagatc actcttgcta aacgccaatc
     1141 ctcatcatct gtaaagagaa tcgacattct tttaaatagt tggccaagta tatcaaatac
     1201 ttcatgaatt ctgtttatag gaaagtctgg atgacagacc acctctgtca gtaaatcggc
     1261 tccatgtgca aaagaatgaa cccaaccata ctgacttgag aaacctgttg tatccttttc
     1321 ttttgaaaga tagtgcaagc cttgatttaa aaggacatta cgaatttctg attgtaatcc
     1381 ctgataaaaa accgattgct ggttagcatc tgcagacaag agatttgcat aaataagtgc
     1441 cctaaaagaa cgttcaagtg ttgacaagcc taccttatca atctctttat ctaaccctcc
     1501 atcagctaag accacctcag caatgaaatg aaattgttcc tgtgtaaata gctcttcctg
     1561 aatctcctta gcaaagcttg taaaaacaag gtcatcacga atttctggag aaggatttcc
     1621 caaatgatca aacaaccact ggatttcttc ctggttataa tttggttttt cttctgctat
     1681 ttttcttagt aaatcttgat acatggtcaa tacctctaca tttctagcaa ctgttcaaaa
     1741 aggcagtctt aaatgacttt accaatttgt aaaattaagt taaaaagaaa aagacattta
     1801 tattcagaga aa
//
LOCUS       DAVLFB010000165         1732 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLFB010000165 DAVLFB010000000
VERSION     DAVLFB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.165"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            73..765
                     /gene="cpsC"
                     /locus_tag="VXD58_002075"
     CDS             73..765
                     /gene="cpsC"
                     /locus_tag="VXD58_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV0676702.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            775..1458
                     /gene="cpsD"
                     /locus_tag="VXD58_002076"
     CDS             775..1458
                     /gene="cpsD"
                     /locus_tag="VXD58_002076"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV0676703.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARTGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTAIGEVNKRDVQKAKQQLEQTEKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
BASE COUNT          578 a          250 c          345 g          559 t
ORIGIN      
        1 gaaaaaagcg aaagaatttt ttgtagataa tcccagaaaa attataatgg atcaattaat
       61 ttaggagaaa atatgaagga acaaaacact ttggaaatcg atgtattgca actattcagg
      121 gctttatgga aaagaaagtt ggtcatttta ttagtggcaa ttataacttc ttcagttgct
      181 tttgcctaca gtacttttgt tatcaaacct gagtttacta gtacgactcg gatttatgta
      241 gttaaccgta atcaggaaga gaagtctggt ttaaccaatc aagacttgca ggcaggatca
      301 tacttggtta aagactatcg tgaaattatc ctatcgcagg atgttttgga ggaagttatt
      361 tctgatttga aactagattt gacgccaaaa ggtttggcta ataaaattaa agtaacagta
      421 ccagttgata cccgtattgt ctctgtctca gttaatgatc gagttcctga agaggcaagc
      481 cgtatcgcta actctttgag agaagtagct gctcaaaaaa ttatcagtat tactcgtgtt
      541 tctgatgtga caacactgga ggaggcaaga ccggcgatat caccatcttc gccaaatatt
      601 aaacgcaata cactaattgg ttttttggca ggggggattg gaactagtgt tatagttctt
      661 cttcttgaac ttttggacac tcatgtgaaa cgtccggaag atatcgaaga tacactgcag
      721 atgacacttt tgggagttgt accaaacttg agtaagttga aataggagag aggaatgccg
      781 acattagaaa tagcacaaaa aaaactggag ttcattaaga aggcagaaga atattacaat
      841 gccttgtgta caaatataca gttgagcgga gataaactaa aagtaatttc cgttacttct
      901 gttaaccctg gggaaggaaa aacaactact tccgtaaata tagcaaggtc gtttgcgcgt
      961 acaggctata aaactctttt gatcgatggc gatactcgaa attcagttat atcaggagtt
     1021 tttaaatcgc gtgaaaaaat tacagggcta acagaatttt tatctgggac agctgattta
     1081 tctcacggtt tatgtgatac aaatattgaa aatttatttg taattcaatc gggatctgta
     1141 tcaccaaacc ctacagcctt gttacaaagt aaaaatttta atgatatgat tgaaacattg
     1201 cgtaaatatt ttgattatat cattgttgat acagcaccta ttggaattgt tattgatgcg
     1261 gcaattatca ctcaaaagtg tgatgcgtcc atcttggtaa cagcaatagg tgaggtgaat
     1321 aaacgtgatg tccaaaaagc gaaacaacaa ttagaacaaa cagagaaact gttcctagga
     1381 gttgttttaa ataaattgga tatctcggtt gataagtatg gagtttacgg ttcctatgga
     1441 aattatggta aaaaataact taggaaagat tttatggatg aaaaaggatt gaaaattttt
     1501 ctagcagtaa tacagagtat tattgtcatt ttattggttt attttcttag ctttgttaga
     1561 gagacagaac ttgaacgttc ttcgatggtt atactatacc ttctccactt ttttgtattc
     1621 tattttagtt cctatggtaa caattttttt aaaagagggt acctagttga gtttaatagt
     1681 actataagat atattttttt ctttgcaata gctataagtg tattaaactt tt
//
LOCUS       DAVLFB010000166         1723 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLFB010000166 DAVLFB010000000
VERSION     DAVLFB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.166"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..723
                     /locus_tag="VXD58_002077"
     CDS             <1..723
                     /locus_tag="VXD58_002077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV0676704.1"
                     /translation="FNILGVAVAIWGGIYGSFFGYELPFHLITTTSDVMTILVVSVVF
                     GFITVFVGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLFIAVGMLMPDIRPLFVLG
                     KWVSIFNAVGILIASIIQAKSLSGIGSGLFNLYNISSYIGDLVSFTRLMALGLSGASI
                     ASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYE
                     GGGKPFQPLKASEKYIKVITKN"
     gene            739..1215
                     /locus_tag="VXD58_002078"
     CDS             739..1215
                     /locus_tag="VXD58_002078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV0676705.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1249..>1723
                     /locus_tag="VXD58_002079"
     CDS             1249..>1723
                     /locus_tag="VXD58_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676706.1"
                     /translation="MSNLENLRESVIEQAHEKGSMKLLDSKKKIDDEFEMQKSVIVKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGL"
BASE COUNT          538 a          234 c          364 g          587 t
ORIGIN      
        1 tttaatatat taggggtagc cgttgcaatt tggggtggaa tctatggctc attttttgga
       61 tatgagttgc catttcatct gataactaca acctcggatg tcatgactat attagtagtg
      121 tcagttgtgt ttggatttat tacagtattt gtaggtttgt tagcttcagg actacaaaaa
      181 gtaagaatga agaaatatgc agaagcatat aattcaggat ttgcgtggtg tgttattctg
      241 cttggcttgt tatttattgc tgtcggaatg ttgatgcctg atattagacc gttatttgta
      301 ttagggaaat gggtatctat ttttaatgct gtggggattt tgattgcttc tattattcaa
      361 gccaaaagct tgtcaggtat tggctcagga ttgtttaatc tatataacat ttcatcttat
      421 ataggtgatt tagttagttt cactcgattg atggcattag gattatctgg agcaagtata
      481 gcatcagctt tcaatttaat tgttggtttg tttccgggga cattggctaa actgacaatt
      541 ggattagtat tattcattct tttacatgcg atcaatattt ttctatcgtt actatcagga
      601 tatgttcatg gagcgcgtct gatatttgtt gaattttttg gtaagtttta tgagggtgga
      661 ggaaaaccat ttcaaccttt gaaggcttct gagaaatata ttaaggttat tacaaagaat
      721 taatggagga tatatataat ggaacattta gcaacttatt tttcaaccta tggaggagct
      781 ttcttcgctg cattgggaat tgtattggcg gttggattaa gcggtatggg gtctgcttat
      841 ggagttggta aggctgggca atctgccgca gctttactga aagaacagcc agaaaaattt
      901 gcctcagctt tgatattgca actattgccc ggaacacaag gattatatgg ttttgttatt
      961 ggaattttga tttggttgca attaactcca gaacttcctt tggaaaaagg cgttgcttat
     1021 ttctttgtag ctcttccaat tgctattgta ggatactttt cggctaagca tcaaggaaat
     1081 gtagcagtag cgggaatgca aatcttggct aaaagaccaa aagaattcat gaagggagca
     1141 attttggctg ccatggtaga aacctatgca attcttgcct ttgtcgtatc attcattttg
     1201 acccttcgtg tataagaaat taatttgcta ttcaaaggag gtgtctaaat gagcaattta
     1261 gaaaacttac gagaatctgt tattgaacaa gctcatgaaa aagggagtat gaaattattg
     1321 gattccaaaa agaagattga tgatgaattt gaaatgcaaa agtcggtcat tgtaaagaaa
     1381 aaagaagctg aacatgaacg aaagttaaaa gaattacaac agaaatatca aataattttt
     1441 caacaattaa aaaataagga acgccaatca acgttagtat caaaacagaa aatattaaaa
     1501 gaactttttc aatctgcttt aatagaaatg gaatcttgga gtgcagataa agaaatggag
     1561 ttcatctatc gaattctgga acgatattca caacaagagg tcatagtaac ttttggggag
     1621 cggactttag ctaaattcaa tttggaacaa ttagagaaat tgaaattctc ttttccaaat
     1681 catttattta gtgaacagcc tatctcaaat gaatcaggct tac
//
LOCUS       DAVLFB010000167         1699 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLFB010000167 DAVLFB010000000
VERSION     DAVLFB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.167"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            100..1128
                     /gene="queA"
                     /locus_tag="VXD58_002080"
     CDS             100..1128
                     /gene="queA"
                     /locus_tag="VXD58_002080"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV0676707.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFY
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
BASE COUNT          488 a          390 c          323 g          498 t
ORIGIN      
        1 ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt ccaaacttga aacgtgttat
       61 aatggatagg attagaactt agaaagaatg aacaaatata tgaatacagc tgattttgat
      121 ttccacttgc cagaggaatt gattgcccaa acgccccttg aaaaacgtga tgcctccaaa
      181 ctcctcatcg tcaaccgtga gacaggagaa atgcaagata aacacttcta ctctattatt
      241 gatatgctgg aacctggtga tgcccttgtc atgaacgaca cccgagttct ccctgcccgc
      301 ctctatggtc aaaaagtgga gacaggaggt catgtggaac ttctcctcct taagaacact
      361 agtggagacg agtgggaagt tctggctaaa cctgccaaac gcctcaaggt cggtactcgt
      421 atcagctttg gtgatggccg cctcagcgct gtcgttacag aagaattgac ccacggggga
      481 cgcattgtcc gctttgaata ccaaggaatt ttcctagaag tcttggaaag tctgggagaa
      541 atgcctctgc caccttatat ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc
      601 tacgccaagg aaagcggctc tgctgcagca ccgactgctg gtcttcactt caccaaagaa
      661 ctgctggcag aaatccaagc taagggtgtt catctagtct atctgactct ccatgtcgga
      721 ctcggaacct ttagacctgt ttctgtggat aatctggacg aacacgaaat gcactcagag
      781 ttctatcaac tttctgagga agctgctgcc acccttcgct ctgtcaaaga aaatggtggt
      841 cgcgtcatcg ctgtcggaac cacttctatc cgcaccttgg aaactattgg ttccaagttt
      901 gatgggcaaa tccaagcaga ttctggttgg accaatatct ttatcaaacc tgggtatgag
      961 tggaaggtcg tggatgcctt ctcaaccaac ttccatctgc caaaatcaac tctggtcatg
     1021 ttggtttctg cctttgcagg ccgtgaatta gtcttagatg cctaccacca tgccatccaa
     1081 gaacactacc gcttcttcag ttttggtgac gccatgttta tttattgaga aagaatttct
     1141 ctaattcttc taataccaat aaatcgctaa gatattattt caaagaacat ctacaattga
     1201 aactctagct agctgtagaa gacgcctagt acattgaaat taaaatgctt ccccctagct
     1261 tcgaaaatat tgccatagat tgcgttgact ctccaaattg attcatctat attttatttc
     1321 agcttcctat attttcttcg ctgtttgtaa atcaaaatgc aagacacatg agtagcacca
     1381 tatttgttac tcttatctgt cctctcaaga gactattatg agttatttca gaatcattca
     1441 ctactttgac cctgactctc cttagtctca aaatcaaaga cttatactct tcaaaaatct
     1501 cttcaaaccg cgtcaacgtc accttggatt atatatgtga ctgacttcgt cagttctatc
     1561 tacaacctca aagcagtact ttgagcaacc tgcgactagt tttctagttt gctctttgat
     1621 tttcattgag tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca gaagactagg
     1681 cttgttcact tttttatag
//
LOCUS       DAVLFB010000168         1696 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLFB010000168 DAVLFB010000000
VERSION     DAVLFB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.168"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1696
                     /locus_tag="VXD58_002081"
     CDS             <1..>1696
                     /locus_tag="VXD58_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV0676708.1"
                     /translation="KLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKSNLS
                     NNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTLGKM
                     DVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRTRQI
                     KEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFFSLL
                     LTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVSGVF
                     LQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQIVAA
                     LKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGANLQ
                     NKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQIIPDG
                     EKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILYKSL
                     FSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVFIL"
BASE COUNT          596 a          234 c          294 g          572 t
ORIGIN      
        1 taaaattatg tatcattgga ttcttctttt gtttgattgc tacaattggt ctggtcacta
       61 ttagtgatac cgaaatcccc atacctttac caattgatgg agctttttct atccaaggaa
      121 aaagtaatct ttctaataac gaaatatacg aaatggttcg tgacttatca aaaacggaaa
      181 aggttaccat ttacaagcca atcgttcaga gttcgggtca gttgaagtat gtaaattttg
      241 atgatgtaaa taatgaacaa ttaaagtcag caccaatagt agggatgtat tacacacttg
      301 ggaaaatgga tgtagacagt ttgaaaccac ttacgatgac gggactacaa acagtctata
      361 tggcttatcc ttggtatata ggaggaatat tacaattcac tggaacttta agaatactgt
      421 taatgggttc aatttattta actttattag ttgttttatt tgttgttagg acgaggcaga
      481 tcaaagaagg agtgatacga cgttctttgg gcttgcctat atacgactta agaagagagt
      541 atggcatttc tcttattttt gaactgatta tgatggcttt attgatgatt tcttacagtt
      601 cttttttggg taatggtttc ttcacctata gttctaagtt atttttctct ctgcttttaa
      661 cgaattttat actatttcaa atcattgatc ttattacttt tgttttattt tggttgacga
      721 ttcagattga aaagcctatt gaaattatta aaaacaaggc aaaaaacaag cttatttttg
      781 tcgtatggct tgctattatt tcgattatca tccttgtctc aggagttttt ttacaagaaa
      841 caaagagtag tcaatctagt attaacatac agatacagaa tttagtacca tgggatacag
      901 taaaagactg gagaaggatt gagttccttg gaattgaaag taactctact aaaaatagag
      961 aagttaatga ttcagatggt cagtatctac agatagttgc tgccttaaaa aacttagatt
     1021 tcttatatat agaacggtct tcagcatatg ttccagattt catgaaaact tcacatgtta
     1081 tagaaaattt ttctaaacaa ttagaaaatg acgggataac gaatccagaa ataaataaag
     1141 agttaattta tatcaaccaa acaggtgcta acctacaaaa taaagtgaat ggaacaaact
     1201 atcatctttt agataataag atagcaacca tctatattcc tgagaagtgg aaagaaaacc
     1261 agaaatctat tgagaataca gttgtagcag aacaatttat tggaacaaac tatacaagag
     1321 aacagcttgc agttcaaata attcctgatg gggaaaaaat tttttatttt aatgaggatg
     1381 ctgataataa tcttaaaatg aaagatattt taccgctggc aaatgtagca gatagcaaag
     1441 ataatatagt tgttgtgtta gatacggaca aaatgatgga aaataataag ttttctttgg
     1501 ctagtaacat actatataaa tctcttttta gtcctgaagc ggtaaaaaaa ataaatgaga
     1561 tgactgtcct gttgaatttc tcaatgaatc cagtagatgt ttaccaaatt gtgaaattaa
     1621 agattcaatc cttagagcac caaattttac tttcacaaat cttgcagaag ataatttaca
     1681 gtattgtctt tatact
//
LOCUS       DAVLFB010000169         1609 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLFB010000169 DAVLFB010000000
VERSION     DAVLFB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.169"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            84..491
                     /locus_tag="VXD58_002082"
     CDS             84..491
                     /locus_tag="VXD58_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV0676709.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VXD58_002083"
     CDS             656..955
                     /locus_tag="VXD58_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV0676710.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VXD58_002084"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VXD58_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0676711.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          552 a          321 c          282 g          454 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcggag
     1201 gaaaagtatt gtactatgat gattcaacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgataca aggaatcgat tcactggctt atctcgttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggatactat ataaacgctt tgagaacgga
     1441 aaactgactt ggcccagtac agaaaaggat gtcaaagctc tcgcacctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacccctact tctaaaacat tgttagaaat cgatttgact gtcctgatc
//
LOCUS       DAVLFB010000170         1591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLFB010000170 DAVLFB010000000
VERSION     DAVLFB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.170"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..304)
                     /locus_tag="VXD58_002085"
     CDS             complement(<1..304)
                     /locus_tag="VXD58_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV0676712.1"
                     /translation="MFAWFLFYEVGSKKDYVSYINPGLWGIAFIVCVLVAFAIFPKME
                     KNFPKTFLYFTKYGKDSLGIYILHAPICSMIRILMLKVGINSVFLHVVVGIVLGWYL"
     gene            complement(699..1453)
                     /locus_tag="VXD58_002086"
                     /pseudo
     CDS             complement(699..1453)
                     /locus_tag="VXD58_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          559 a          307 c          243 g          482 t
ORIGIN      
        1 ataaatacca gcctagcaca atcccaacaa caacgtgaag aaaaactgag tttattccca
       61 ctttcaacat tagaatccga atcatgctac aaattggtgc atgaagaata tagatcccta
      121 aactatcttt cccgtattta gtgaaatata ggaaagtttt aggaaaattt ttctccattt
      181 taggaaaaat cgcaaaagca actaatacac agacaataaa agcaatcccc cacaaacctg
      241 ggttaatata gcttacataa tccttcttag accctacttc ataaaacaag aaccaagcga
      301 acatataaat aaaatcaaat aacacaaaga aaaaaagaaa ttttttcaaa ttaattttat
      361 caaagtgaat ttcactcaat acactgccaa gaaagaaaca aatcccccaa accaacgtcc
      421 gttgcacaat aaaaatatca gtttgaataa atagtgtgaa aatataggca aaaacactaa
      481 ctaacaaaag ggacttacga tttttgaaaa caacagatag taaaccataa actatcaata
      541 tcgaccataa cgtatataga taccatgaaa ctcccagagg aaatttatag atattcaata
      601 ggttaaatat catagtgggt actcggacag aggcacccgc aaccttttga atacaaaagt
      661 gaataattga ataaaaaata tatagtatgc ctaaaatatc cgaagcgttt acgatgattt
      721 cgataggttg ttgaaaacat tttaaacgtt tttactttgg caaggatgtt ctcaaccttg
      781 cttctatcct tagacagtgc atggttatag gctttatctt cagctattag cggcttgagt
      841 ttgctggatt tacgtggaat ttgtgcttga ggatatatct tcatgagccc ttgataacca
      901 ctgtcagcca agattttacc agcttgtccg atatttctgc gactcatttt gaacaacttc
      961 atatcatgac aatagttcac agtgatatcc aaagaaacaa ttctcccttg acttgtgaca
     1021 atcgcttgag tcttcatagc gtgaaatttc tttttaccag aataattcgc taattctttt
     1081 tttagggcga ttgattttta cttccgtcgc atcaatcatt accgtgtcct cagaactgag
     1141 aggagttctt gaaatcgtaa caccaccttt gaacaagagt tacttcaacc cattagctcc
     1201 gacggattaa gttgctttcg tgaataccaa aatcagccgc aatttgttca taagtgcggt
     1261 attctcgcac ttattgaaga gtcgccataa gaaagtcttc taggcttaat ttaggttttc
     1321 gtccaccttt tgcgtgttta agttgataag ctgtttttaa tacagctaac atctcttcaa
     1381 aagtcgtgcg ttgaacacca acaagacgct taaatcgtac atcggttagt tgtttacttg
     1441 cttcataatt catagaacta ttataccata ttttgtttcg caggaagtct attatataaa
     1501 ccaacttaga taatagacca cgaaaatgtc ttaaccggaa aacaccttat gaagttttct
     1561 atgaaaaaaa tgtgcactta atttgacaat t
//
LOCUS       DAVLFB010000171         1591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLFB010000171 DAVLFB010000000
VERSION     DAVLFB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.171"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..718
                     /locus_tag="VXD58_002087"
     CDS             <1..718
                     /locus_tag="VXD58_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0676713.1"
                     /translation="NYIINYGTLIGAVRHEGFIPWDDDIDLSMPREDYQRFINIFQKE
                     KSKYELLSLETDKNYFNNFIKITDSTTKIIDTRNTKTYESGIFIDIFPMDRFDDPKVI
                     DTCYKLESFKLLSFSKHKNIVYKDSLLKDWIRTAFWLLLRPVSPRYFANKIEKEIQKY
                     SRENGQYMAFIPSKFKEKEVFPSGTFDKTIDLPFENLSLPAPEKFDTILTQFYGDYMT
                     LPPEEKRFYSHEFHAYKLED"
     gene            720..1529
                     /locus_tag="VXD58_002088"
     CDS             720..1529
                     /locus_tag="VXD58_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV0676714.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          534 a          286 c          283 g          488 t
ORIGIN      
        1 caactatatt attaactacg gtactctgat tggggcggtt cgacatgagg gctttatccc
       61 ttgggacgac gatattgatt tatccatgcc tagagaagac taccaacgat ttattaacat
      121 ttttcaaaag gaaaaaagca agtatgaact cctatcctta gaaactgata agaactactt
      181 taacaacttt atcaagataa ccgacagtac gactaaaatt attgatactc gaaatacaaa
      241 aacctatgag tctggtatct ttatcgatat tttccctatg gatcgctttg atgatcctaa
      301 ggtcattgat acttgttata aactggaaag cttcaaactg ctgtctttca gtaaacataa
      361 aaatattgtc tataaggata gccttttaaa agattggata cgaacagcct tttggttgct
      421 ccttcgacca gtttctcctc gttattttgc aaataaaatc gagaaagaaa ttcaaaaata
      481 tagtcgtgaa aatgggcagt atatggcttt tatcccttca aaatttaagg aaaaggaagt
      541 cttcccaagt ggtacctttg ataaaacaat cgatttaccc tttgagaatt taagccttcc
      601 tgcacctgaa aaatttgata ctattttgac acaattttat ggagattata tgaccctacc
      661 accagaagaa aaacgcttct acagtcatga atttcacgct tataaattgg aggattagga
      721 tgcaatattt agaaaaaaaa gaaattaaag aaattcaact agccctgctg gactatattg
      781 atgagacttg taagaaacat gatattcctt attttctcag ttatggaacc atgcttggag
      841 ccatccgcca caaaggtatg attccttggg atgatgatat tgatatttcc ctttatcgtg
      901 aggattatga gcgtttactg aagattattg aagaagaaaa tcaccctcgc tacaaggttc
      961 tttcctacga tacatcttct tggtacttcc ataatttcgc atcgattttg gacacttcta
     1021 ctgttataga agaccatgtt aagtacaagc gtcatgatac cagccttttc atcgatgtct
     1081 tcccaattga tcgatttaca gacttgagca ttgtcgacaa gagctataag tatgtggctc
     1141 ttcgtcaact agcttatatc aaaaaatcac gagcagttca cggtgatagc aaactaaaag
     1201 attttcttag attatgtagc tggtacgctc tccgatttgt caatcctcgc tacttttaca
     1261 agaaaattga tcaactagtc aaaaatgctg taaccaacat tcctcaatat gaaggaggag
     1321 ttgggatcgg taaggaaggg atgaaagaaa tcttcccagt tgataccttt aaagaactga
     1381 ttttaactga gtttgagggc cgtatgttgc ctgttcccaa aaaatatgac caatttttaa
     1441 cccagatgta tggcgattat atgacaccac catcaaaaga aatgcaagag tggtatagtc
     1501 atagcattaa agcttatcgc aaaaactgat tgagggggat tatacaaact actaagatag
     1561 aggttattca aaaacataat tttagtagaa a
//
LOCUS       DAVLFB010000172         1557 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLFB010000172 DAVLFB010000000
VERSION     DAVLFB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.172"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..234
                     /locus_tag="VXD58_002089"
     CDS             <1..234
                     /locus_tag="VXD58_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0676715.1"
                     /translation="IFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYPH
                     MALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            234..929
                     /locus_tag="VXD58_002090"
     CDS             234..929
                     /locus_tag="VXD58_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV0676716.1"
                     /translation="MSTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            917..>1557
                     /locus_tag="VXD58_002091"
     CDS             917..>1557
                     /locus_tag="VXD58_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676717.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLID"
BASE COUNT          534 a          209 c          292 g          522 t
ORIGIN      
        1 atcttcagca catggatatg tagctcttta atgcaaatgc ttttagcaaa agaaaaatat
       61 atggagtggt ctttgatgac gatgggaaaa gattatgttg atgtattaga aaagttaata
      121 acttatcctc acatggcttt agtagcctta ggtgctttct taggaggaat tcttggagca
      181 tatataggca aggctctatt gaaaaaacac tttgaaaaag caggcattgt ataatgtcta
      241 ctttttcttc aaatgctccc tttaatccca atccgatctc taagatatta gtcgtattta
      301 tcacaggctt aactgttatg catagcataa acatccgttt tgagctggca attgtttgtc
      361 ttattggtat tttgctttat ttgaatggat acaaaaaaac acttttcaaa tggattatat
      421 tgtgtgggat actttactcc ttgcctaatt ttatggtgtt atctgaatta aaccctatag
      481 ttaagatgtt tttgagtata cctattgtta ttagaatgtt tattttacca tttatggcag
      541 caagctttat gataaagacc tcggatgtag gcgcaataat ttcatcgatg gataagctta
      601 agatttcaaa gaatgtatcc atacctattg cggttatgtt tagattcttc ccatctttta
      661 aggaggagaa gaaaaacatc aaaatggcta tgagagtaag agggataagt tttaaaaacc
      721 cagtcaaata tcttgaatat gtttctatgc cactactcat tatatcatct aatatatcag
      781 atgacattgc aaaagcggca gaaacaaagg caatagaaaa tccaattgcc aagaccagat
      841 acattcgcgt aaagatacag ctaattgatt ttgtttatgt tttagcggtt gctggactta
      901 ttgtgggagg cttaatatgg ttgaaataaa aaatttaagt cttgattatg gtgaagagca
      961 tatattagat gatatatcac tatccatagc cgagggagag tgcgtgctat ttacaggaaa
     1021 aagtggaaat ggtaagtcat ctttaataaa ttcaatcaat ggactagctg taaggtatga
     1081 taacgcaaag acaaagggcg aaataattat tgatggtaag aatataaaaa atttggaact
     1141 ttatcaaatc tcaatgcttg tttcaactgt ttttcaaaat cctaagacat atttttttaa
     1201 tgtcaatacg acattagaat tattatttta tttggaaaat atcggtcttg caagagaaga
     1261 gatggacagg cgtttgaagg atatgcttaa gatattcccg ataaaaaatc ttttgaacag
     1321 aaatatattt aatctatccg gcggtgaaaa acaaattctt tgcattgcag cttcttatat
     1381 agcaggtaca aagattatag ttatggatga gccttcatcg aatttagata ttaaaagcat
     1441 aaatgttttg gcaaagatgc taaagatatt aaaagagaaa ggcataagca taattgttgc
     1501 agagcataga atttattatt tgatggacat agttgaccgt gtatttttaa tagataa
//
LOCUS       DAVLFB010000173         1556 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLFB010000173 DAVLFB010000000
VERSION     DAVLFB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.173"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(156..720)
                     /locus_tag="VXD58_002092"
                     /pseudo
     CDS             complement(156..720)
                     /locus_tag="VXD58_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(796..1008)
                     /locus_tag="VXD58_002093"
     CDS             complement(796..1008)
                     /locus_tag="VXD58_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0676718.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1017..1322)
                     /locus_tag="VXD58_002094"
     CDS             complement(1017..1322)
                     /locus_tag="VXD58_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV0676719.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1309..1503)
                     /locus_tag="VXD58_002095"
     CDS             complement(1309..1503)
                     /locus_tag="VXD58_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0676720.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          442 a          326 c          290 g          498 t
ORIGIN      
        1 tccacgtttg gaagacattc tagaagatag gatagatatt tctcactatt tataatggat
       61 tgaaataaga tatgaacaaa tcgattagga aaggaaaatt gatttataga aatgttttag
      121 cagtcgatgc gtactgttat agattcaacg cactataatt gatgacgttt agctgtttcc
      181 aacaaactca taataatagc tcttgcttta gctccttcaa aactttgaga aaataaccac
      241 cctcgctttt ttaaactgag cttaggctaa aagagtccac tggactcttt tactccgtcc
      301 cataaccaat gatttaatgg cgcattcagc tagattattg gaaaggacca gatgtccgtc
      361 tttcaaaata gtcttaaagg tttctttata cttgaggcta tacttgaggc tgtattcaat
      421 tgcccttcct agttttgaac ccgataaaac tgactgacgc cggcaccaag caaagaaatc
      481 ttccattagg ggttggagat gttcttgacg tttctgtagt cgttcatcag ctggcaaagc
      541 ctcccagttt ctttccaagg aaaataactg attacaataa gctaaacctt tagctcctaa
      601 ggatgattta ttcgcttgct tgggggtcgc ttcaaaaaac ttccttctca catgtaccca
      661 acatccaaca agttcagctt cttccagttg acaataagct tctagcggtt cgcagtttac
      721 caagcttagc gtcgccttgc tcctaaacct tggactgcta tcgcataggc agaactagag
      781 gactaaagtc ctaagttact gccgcaacat atcacaatgc acatagccag aataatctcc
      841 taggaattct tgcactaccg aaccactccg acgctgatca tggtggtaca gcgtgattgc
      901 ttgattctca gctttcccag acaaaaaggt ccaatagtag gtcagatggc tatcattctc
      961 tagaacccga taggaggttt catctgcatg aagtaagggc tgaatcaata gtctcgttat
     1021 aaaggggctt taggaatagg agcttttaca attttatcac ttggattatt cttactgcat
     1081 gtttggcatt tgtaagcgtg ttggatatga tccactcttt ttaattgcgc aggaataaag
     1141 accaattctt gtcgttgaag gctcgctcca atctctttta aatcgtcctg acaatcaggg
     1201 caaatgctct cttctacttg atgatgaact tcttctgaat caaattgggc aagaatagct
     1261 tgacgtttcc ctttagattt cttacgtttg taggtaattt cctctgtttc aactgggaac
     1321 atctccatct ttctcagatg gagactcttc ctcaaaaagg ttgagttgtc caaaagggca
     1381 aacacttttc tcagaggatt ttccagagag cttttgcgtt agataagcca cttgttcaca
     1441 cagaagggaa agttcattag tgagactatc aattatagca ctctgttgtt gaatgatttt
     1501 caatcttctc ccatagactt tcctccttat ttcctaactt tattatatag tgtatt
//
LOCUS       DAVLFB010000174         1487 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLFB010000174 DAVLFB010000000
VERSION     DAVLFB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.174"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..1160)
                     /locus_tag="VXD58_002096"
     CDS             complement(<1..1160)
                     /locus_tag="VXD58_002096"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV0676721.1"
                     /translation="MAISQMKRISLLFTKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFSTIQISHTSNSHHGVIEGNDALNYLMNKQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERNIQSNNEEIERLIKW
                     EKLEIIPAHLEQFSFCRGKVGTIPRTVDNRLYNNFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTF"
     gene            complement(1150..1473)
                     /locus_tag="VXD58_002097"
     CDS             complement(1150..1473)
                     /locus_tag="VXD58_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676722.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELISIESNLEMKQQNLIDEVNKTIKENDAKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
BASE COUNT          456 a          265 c          193 g          573 t
ORIGIN      
        1 aaaaatgtaa agtaaaatgg tgctaataca ggtgtaggat ctttctcata atatttgggc
       61 agagcataca tttctgtcaa taattcaaat ggttcaatta aagagtgatt cgttaatttg
      121 ataggtattt catcttgatt atccgacaat gtttccgatt cataaatata aatagaatgt
      181 ccaaaacgct catccataac ttttataaaa tataaaatac gagtttcttc tatccatcct
      241 tctaatgcaa ctagatgcgg agtgctcaaa aggttattct tagagatttg acgagcatag
      301 atagataaaa tatagtcaat ttgccattgt aaatctatca aatttttctt tgaatcttgc
      361 aacgatgcga taatattctc tttttcagtt attaactgca tgttttcttg atccaaatct
      421 actactcgct gcttcggtaa ttcctttcta gaataatcaa atggttcaaa taagtattca
      481 tcaaaatcaa tagagcaact ggactgatag aaaacaacaa caccgtactc tctatcatta
      541 gaaaatattt cttgaacttc aatattgttt tctaaaaaat tattgtataa gcgattatct
      601 acagtccttg gaattgttcc gacttttcct ctacagaaag aaaattgttc taaatgcgca
      661 ggaataattt ctaatttttc ccactttatt aatctctcta tttcctcatt attactttga
      721 atgtttcttt ctaattcatg aactctgttt atttgatgat tcactttttt aagaacaccc
      781 tcagcaacat tagctttacc aaatttttct aattcttcat aagaggttgt tatcggaggt
      841 tgctgtaata acttaaacgt gttttctttc ggtaggtatt cttgtaattt ctctacagtt
      901 gcttccaaat gttgttgttt attcatcaaa taattcaagg catcatttcc ttcaataacc
      961 ccatgatggg aattagaagt gtgagaaatt tgaatagttg aaaatacaac ctcatctttt
     1021 tctagcgctt ctgaccagtt atcctgaacc tttaaatcac ggaactgcac tgactctagt
     1081 tcttgaatag tttttaaaac atcatcaaga ctactcttag taaatagtag agaaattctt
     1141 ttcatctgac taatagccat atttttctac caccctacca ataatttttt ctacaaattc
     1201 tcctctccta cttgatgaaa tatactgaat ctttgcatca ttttccttga ttgttttatt
     1261 aacctcatca atgaggttct gttgtttcat ttctaaattg ctttctattg aaattaattc
     1321 ttcgtgactt ttttcatcat aacttttttc tagtttttcc aattcttctt gggagacagc
     1381 tttatacgta taaatctcat tatcatatga ttttataata tcggttgcaa cattctcaat
     1441 atcttgcatt gtcgaaaata cagagttagt cattgtgaca cctcctt
//
LOCUS       DAVLFB010000175         1436 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLFB010000175 DAVLFB010000000
VERSION     DAVLFB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.175"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(137..898)
                     /locus_tag="VXD58_002098"
     CDS             complement(137..898)
                     /locus_tag="VXD58_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV0676723.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELFFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(898..1374)
                     /locus_tag="VXD58_002099"
     CDS             complement(898..1374)
                     /locus_tag="VXD58_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV0676724.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
BASE COUNT          430 a          286 c          197 g          523 t
ORIGIN      
        1 aaacaggtgt atgcaaatga attaaagtct tgtgaaagct accgaatttc tctttggaat
       61 ttgttagttc ttgataaaca gactttatct tgttattaaa ccctctcatt tctattctcc
      121 taaaaatatc aattgtttat ttttcaagta attcattaag tctcttttcc cccacctgca
      181 acatctcctt ctctacctga ctccactccc caaagaataa ctcttgaaga acatctgctg
      241 ctgaagttgt attttctttt gaatcatata cacaacttct atctcgttgg taaatttgaa
      301 ttctttcaaa gatagctagt tcttccaatt ttcgtgtgtt atcaactaga tgatttacaa
      361 tgaaatcatg atgttctttt ggagttgcgc gtgcttgatt tggattgata atgtacagtt
      421 cttcataacg gataagagta cttagatacg acaattcagg ctttgtcgca attaaggcca
      481 attgtacttc atatccctta tttttcaaga gttgtgccgt tttctttgga acatcaattg
      541 ttcgtaaagt tccctcgatc aaaagattgt atcccaaact actcaatttt gttactaaag
      601 actctaccat ttttcctgca aaatctttgg tatattctac actgtctttg ccatattctt
      661 gctgcagttc taaatagtgt ggatgctgag aacgaaaact atcaccatct atgataacaa
      721 tatttccttg aaattctttc tgtttaatac gatgaattgt agtcttaccg gcaccacttt
      781 gacctccaag caaaatcgct ataggttgct tactggactt ttttcctctt gtcagtgaac
      841 gaagattcct tgctaaagca tgtttgaatt cactatcagt ataatcttgg atttccatta
      901 agctaccatc cgcttttcag atatctctaa catacgttca attccatcca aatagccact
      961 atatctctct atttcatcaa aagtttctac taaatagata ttcgtatgaa tcaaatcaga
     1021 aagatcatca ctcattaaaa tccaaggatt agattcatca tcaatgctaa ttccctgact
     1081 atcttgatag cgatagagtc gagataatag attagcacct ctttctttca caatttcaat
     1141 ttttaaagtc aattcataat cttcaacagg attgagcatt ttatcttctc ctacgatatc
     1201 gacataagat acattaaact tctgacaaat gatgtctatt aattcggtag agactgaact
     1261 cgttccattt tcataacgac tcagactatt tcgtgaaata cctacaatcc gtgcaaattc
     1321 gtgttgtgtt aagtcatgtg ttttacgtaa ggattttatg ttctttccaa tcatggcaaa
     1381 ctcctttatt tttataattc aattatagca taaaaaagca taatgcacca aaaatg
//
LOCUS       DAVLFB010000176         1400 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLFB010000176 DAVLFB010000000
VERSION     DAVLFB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.176"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..>1400)
                     /locus_tag="VXD58_002100"
     CDS             complement(<1..>1400)
                     /locus_tag="VXD58_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV0676725.1"
                     /translation="QSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQIS
                     NISNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANADSQFVNVTN
                     QLARKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEVGKVAKGGR
                     NYIRNGQFKNGSKNWLEYQSVNFGLNFNYQHSQNPNNRNRPGLHFYHDSQDVANFFGI
                     QQSFAFDGVRGEKVSVSLLVSKDGGDSNSGLKVALHYIKNKNIIGQEWQNIPSPQITS
                     KYKRFTFTFTLSDDVENLNLMLFGEKGKTINLYVTDVQLERGSVATDYKEAPEDTDEA
                     IRSVQSQLAGSWAVENINSAGDIISGINLGANGHNRFVGKLTHITGETLIDRAVIKSA
                     MVDKLKTANFEAGSVTTTILEAEAVTAEKLKVDNALIKKLTATDAFIDQLTSKRIFSI
                     KIESVISSSTFLEAYQG"
BASE COUNT          382 a          300 c          261 g          457 t
ORIGIN      
        1 ccttgatagg cttctaggaa ggttgagcta gaaatgacgg actcaatctt aatagagaag
       61 atacgtttag atgtcagttg gtcaataaaa gcatcagttg cagttaattt tttaataagc
      121 gcattgtcaa ctttcaactt ctcagcagtt accgcttcag cttctaatat cgtagtcgtg
      181 accgaaccag cttcaaaatt ggccgttttg agcttatcaa ccatggcaga cttgatgact
      241 gctctgtcaa tcagggtctc tccagtgatg tgggtcaatt tcccaacaaa gcggttatgt
      301 ccattggcgc caagattgat tccagagatg atatctccag ccgagttgat gttttcaact
      361 gcccatgagc cagctagttg gctttgaaca gagcgaatcg cttcgtccgt gtcttcagga
      421 gcttctttgt agtccgtcgc gacagaacct ctttcgagtt gaacatctgt aacatagagg
      481 ttgatggtct tccctttttc accaaacaac atcaaattca gattctctac atcgtctgat
      541 aaagtaaacg taaatgtgaa acgcttatac ttcgatgtta tttgtggact tgggatattt
      601 tgccactctt gcccaataat gtttttgttt ttgatgtagt gcaaagcgac tttcaagcca
      661 ctgttgctat cgccaccatc tttcgaaaca agaagagata cactcacttt ctcacctcga
      721 acaccatcaa atgcaaaaga ttgctgaatt ccaaaaaaat tagcaacatc ttgagaatcg
      781 tgatagaagt gtagtcctgg acgatttcga ttattaggat tttgagagtg ttgatagttg
      841 aaattcaaac caaaattaac agattgatat tcaagccagt ttttcgaacc gttcttaaat
      901 tgaccatttc tgatataatt tcgaccacct ttggcaacct tcccaacctc aacctgaaac
      961 agttgattag tcagagccat gcgagcaacc ttatccgcaa ttccattttc agtattgccc
     1021 aaaatccgct cgtaaagttt actggtttcc ttaacacgct ggaagtcagt agtctctact
     1081 tttcgtgcta gttgattggt cacattcaca aattgactat cagcattcgc tttgtttgca
     1141 gaaacctgat cagatattct acccatttgt cgttcagcat tatccttgtt tgtagcgacc
     1201 tgagtcttta aatttgaaat ctgattatct gtgccttgct tattactgtt tatccgattt
     1261 gaaatatttg aaatctgagt agtggttcct tgctcactgc ttgtaagtct atttgataga
     1321 ccactgattt gaccgcccac atcttgctta taagtagtta tctgacttga aatatccgtg
     1381 aacttaccat ctacagattg
//
LOCUS       DAVLFB010000177         1393 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLFB010000177 DAVLFB010000000
VERSION     DAVLFB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.177"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            3..719
                     /locus_tag="VXD58_002101"
     CDS             3..719
                     /locus_tag="VXD58_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676726.1"
                     /translation="MKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILGISGSGKST
                     LLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIPSLPVIENI
                     ALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAVIADSDIIF
                     ADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILKDGKIFKEL
                     HRPSGEELYKILEVQSTTEE"
     gene            722..>1393
                     /locus_tag="VXD58_002102"
     CDS             722..>1393
                     /locus_tag="VXD58_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676727.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQ"
BASE COUNT          445 a          187 c          279 g          482 t
ORIGIN      
        1 aagtgaaagg gctgtatcat caatttttac taggaaataa taaaacgttg caagtgctga
       61 aaaatgtttc tctttctgct tcgagaggag aatttataag tattctagga attagtggtt
      121 ctggaaagtc aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag
      181 taattttaaa tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac
      241 gtaacgaagt atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag
      301 aaaatatagc acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa
      361 acttactcaa aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg
      421 gtggagaaca acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat
      481 ttgctgatga gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat
      541 tgagagagtt agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg
      601 cctcgaaaac tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata
      661 gacctagcgg ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga
      721 gatgctcaga ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc
      781 accagtaatt tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt
      841 tgagaataat tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc
      901 gatagtattc ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt
      961 tgaaattttt agagatgatt acgaacttct ggaggtgttg ggagcaagtc tccttcaact
     1021 atcatttctt gtggggggac agatttttat catttcctcg attattagtt ttattgcata
     1081 tctatgctct atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga
     1141 aaatatatta cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt
     1201 gttgatttca tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa
     1261 taggaagagt tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc
     1321 tggttttagt gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt
     1381 agcgaaggcc cag
//
LOCUS       DAVLFB010000178         1382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLFB010000178 DAVLFB010000000
VERSION     DAVLFB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.178"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(68..155)
                     /locus_tag="VXD58_002103"
     tRNA            complement(68..155)
                     /locus_tag="VXD58_002103"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Ser,seq:gct)
     gene            complement(162..235)
                     /locus_tag="VXD58_002104"
     tRNA            complement(162..235)
                     /locus_tag="VXD58_002104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Ile,seq:gat)
     gene            complement(271..341)
                     /locus_tag="VXD58_002105"
     tRNA            complement(271..341)
                     /locus_tag="VXD58_002105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Gly,seq:tcc)
     gene            complement(361..433)
                     /locus_tag="VXD58_002106"
     tRNA            complement(361..433)
                     /locus_tag="VXD58_002106"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Phe,seq:gaa)
     gene            complement(437..510)
                     /locus_tag="VXD58_002107"
     tRNA            complement(437..510)
                     /locus_tag="VXD58_002107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(474..476),aa:Met,seq:cat)
     gene            complement(521..610)
                     /locus_tag="VXD58_002108"
     tRNA            complement(521..610)
                     /locus_tag="VXD58_002108"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Ser,seq:tga)
     gene            complement(621..694)
                     /locus_tag="VXD58_002109"
     tRNA            complement(621..694)
                     /locus_tag="VXD58_002109"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(658..660),aa:Ile,seq:cat)
     gene            complement(708..781)
                     /locus_tag="VXD58_002110"
     tRNA            complement(708..781)
                     /locus_tag="VXD58_002110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Met,seq:cat)
     gene            complement(787..860)
                     /locus_tag="VXD58_002111"
     tRNA            complement(787..860)
                     /locus_tag="VXD58_002111"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(824..826),aa:Pro,seq:tgg)
     gene            complement(876..949)
                     /locus_tag="VXD58_002112"
     tRNA            complement(876..949)
                     /locus_tag="VXD58_002112"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(913..915),aa:Arg,seq:acg)
     gene            complement(959..1044)
                     /locus_tag="VXD58_002113"
     tRNA            complement(959..1044)
                     /locus_tag="VXD58_002113"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1008..1010),aa:Leu,seq:taa)
     gene            complement(1052..1123)
                     /locus_tag="VXD58_002114"
     tRNA            complement(1052..1123)
                     /locus_tag="VXD58_002114"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1089..1091),aa:Gly,seq:gcc)
     gene            complement(1146..1218)
                     /locus_tag="VXD58_002115"
     tRNA            complement(1146..1218)
                     /locus_tag="VXD58_002115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1183..1185),aa:Thr,seq:tgt)
     gene            complement(1237..1318)
                     /locus_tag="VXD58_002116"
     tRNA            complement(1237..1318)
                     /locus_tag="VXD58_002116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1282..1284),aa:Leu,seq:tag)
BASE COUNT          382 a          387 c          302 g          311 t
ORIGIN      
        1 aagtttacgg atctgaagtt ttattgtctc taaaaacaaa aaagagagtt acaaactctc
       61 attaaaacgg agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag
      121 caaaccgtcc tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga
      181 accaccgacc tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt
      241 aaaaaacttg gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa
      301 cagcttggaa ggctgtagtt ttaccactaa actacacccg cataaatact atcaataaaa
      361 tggcgcgaga cggaatcgaa ccgccgacac atggagcttc aatccattgc tctaccaact
      421 gagctaccga gccttattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc
      481 cgacgagcta ccgagctgct ccatcccgcg ttaatataaa aggaggatgt gggattcgaa
      541 cccacgcacg cttttacacg cctgacggtt ttcaagaccg ttcccttcag ccggacttgg
      601 gtaatcctcc aatattcaaa tggaccttgt aggacttgaa cctacgacca ctcggttatg
      661 agccgagagc tctaaccagc tgagctaaag gtccgacaag atcattatag cggcgaaggg
      721 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      781 catgaatcgg gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta
      841 ccaagctgag ctacttcccg agttaaatag aaaaatgcac cctagaggag tcgaacctct
      901 aaccgcctga ttcgtagtca ggtactctat ccagttgagc taagggtgct ccatattatg
      961 ccgaggaccg gaatcgaacc ggtacgatcg ttaccaatcg caggatttta agtcctgtgc
     1021 gtctgccagt tccgccaccc cggcctctct aagcgaacga cgggattcga acccgcgacc
     1081 cccaccttgg caaggtggtg ttctaccact gaactacgtt cgcactgttt tcttctatct
     1141 aaaaatgccg gctacatgac ttgaacacgc gaccctctga ttacaaatca gatgctctac
     1201 caactgagct aagccggctc atttgttata tcttaatgcg ggttaaggga cttgaacccc
     1261 cacgccgtta agcgccagat cctaaatctg gtgcgtctgc caattccgcc aaacccgcat
     1321 atatgacccg tactgggctc gaaccagtga cccattgatt aaaagtcaat tgctctacca
     1381 ac
//
LOCUS       DAVLFB010000179         1353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLFB010000179 DAVLFB010000000
VERSION     DAVLFB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.179"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1353
                     /locus_tag="VXD58_002117"
     CDS             <1..>1353
                     /locus_tag="VXD58_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV0676728.1"
                     /translation="NNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTS
                     PSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKE
                     AAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFETNHPAI
                     KHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGY
                     GRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDD
                     LNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVR
                     RLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTP
                     GDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLS
                     "
BASE COUNT          523 a          183 c          254 g          393 t
ORIGIN      
        1 caaataatga gtggtttaaa gaacaaacta aagcatatat tgtagaagaa aaatctacta
       61 ttaaagaggt aagtgataag caatcaatag ctggtagtcc atattcaatt ggagtatatg
      121 atagattaac tagcccatct tggaaatatc caagtatggt gttgccgcta ttaacattac
      181 ctgaaaaatc ggtgtttatt atagccaaca tttcgactat aggtttcggt gcttatgata
      241 gatatagaag taaagaacat ccagcaggaa ctgatttaaa tgactatgtt gagaaaaaag
      301 ctaaagaagc ggctgttaga tttagagatc actatgatta ttggtataga atactagatg
      361 ataaaaataa agaaaaatta tatagaagtg ttctagtcta tgatgcattt agatttggta
      421 cagatgaaaa agaagataaa gatacatatc aagcaacttt tgaaactaat catccagcta
      481 taaaacattt cttcggtcca gcgggaaata atgttgttca taattctaat ggagcatatg
      541 ctacagggga tgcattctat tatatggcat atcgcatgct tgataaagat ggtgcggtaa
      601 cttatacaca cgaaatgaca cataactcag atagagaaat ttatcttgga ggctatggaa
      661 gacgaaatgg acttggacca gagttttatg ctaaaggact gctacaagct cctgatcatc
      721 ctaatgatcc aacagtcact attaattcca tattaaaata tgatcaatca gaagaatcaa
      781 caaggcttca agtagcagat ccaactcaac gatttggaag tgttgatgat ctaaataagt
      841 atatgcataa tatgtttgat gtaatataca tgcttgaaat actagaaggt aaagctgtag
      901 ctaaattaga tacaaatcaa aaatatgatt tattaagaaa aatagaaaat gaatataaac
      961 ctgatcctga tggaaattct gtttatgcaa cgaatgttgt acgtagatta aaaccagaag
     1021 aacttactaa attaactaca tttaacagtt tgatagaaca tgatattata acaagaagag
     1081 gttatgtaga tgaagcaact tataagcgga atggctacta tacaattaat ctattctcac
     1141 caatttattc agcattgagc agtaaaatag gaacaccagg agatttaatg ggacgacgta
     1201 ttgcatttga gttattggca gccaaggggt acaaagacgg tatggttcca tatatttcta
     1261 atcagtatga aaaagaagcg aaagctcaag gaaaagttat tacatcatat ggtaaacaaa
     1321 taggtttagt tactgatgaa attgttcttt caa
//
LOCUS       DAVLFB010000180         1353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLFB010000180 DAVLFB010000000
VERSION     DAVLFB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.180"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..>1353)
                     /gene="lanM"
                     /locus_tag="VXD58_002118"
     CDS             complement(<1..>1353)
                     /gene="lanM"
                     /locus_tag="VXD58_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEV0676729.1"
                     /translation="IVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENILNFY
                     NKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQ
                     GKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEE
                     FIDYLPLEQKKKLPEYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQ
                     PLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLI
                     NLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEF
                     KMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQG
                     IVASECEQMRLLDIPIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENL
                     "
BASE COUNT          463 a          252 c          151 g          487 t
ORIGIN      
        1 tctaaatttt ccaaattatt attaggtaaa tatttcataa aatagtcata tccagaaaat
       61 tgatgtaggg aaataaaatg atttccaaaa tcatcgtaga tttcattgat atttgtatct
      121 gtataaaaaa tcggaatatc taataacctc atttgttcac attcgcttgc tacaatacct
      181 tgattagaaa acttattgct ccagagattt tccaatgctt tttctctatc taacatttct
      241 tcataaaaat caggatgata taaaaaagat agtactgaag catagctatt tgtgtctcta
      301 aaaagtaccc ttgtctttaa accatacaag tttgctttta atagcatttt aaattcttct
      361 gttttattta actcttcaaa tatcagataa aaatccctaa aacctttttt gaaatctttt
      421 atatacttat caaattctat atcaccatcc cgaacaggca ggtttttccc accttcaaaa
      481 tcaattttcc caatatcaaa ctttacctta tcagtattta aattaattaa aacttgacca
      541 ttaaaagcat ccttcttaaa atttcctgta agagcactca tctcaacatc aagaccatcc
      601 tcccttttta ctccctctgt tggaagaagc aaagttcgag tcaccctgtg aaacagggta
      661 tccagcaaac tctgaccagt tttatcatca aatagaggtt gctgtaacat tgtttcaaag
      721 tctattatta caggcatatc tccataggaa attaaatttt caaaatgtat atcactccca
      781 ttaaataaat atataaatgc taaaagcaca ccaaaattat aataatattc aggtaatttt
      841 ttcttttgct ctagaggtag ataatcaata aattcttcat agctgtgatc cgaataagca
      901 atagttcgag gtattcttat cctaattttt tcatcaacct ttcggatata ctcagcaata
      961 gtatttagtg agttattgat tattaaattt ttaggcttgt aaacaatttt cgcgtcatca
     1021 aagaagagta tcaaaacagt ttttccttgg ctgtgtgtat caccctttcc aaattttata
     1081 tcatttaatt tcccccttag ccctaattct tcttctagca cactaaaatc attttctaaa
     1141 tttgataaaa tttctataaa ataagtatag aaccgaagac aagactccga tatttgcctc
     1201 agtaaaacgg gatatttgtt ataaaaattt aatatattct cttccttgtc aaacaatgtt
     1261 ataaaatatt tgtaccgttg ctgctccgat tccccttcaa gtaaaccttt ttcacgcaat
     1321 gaatttatct ctaaaaccaa aggtttaaca atc
//
LOCUS       DAVLFB010000181         1352 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLFB010000181 DAVLFB010000000
VERSION     DAVLFB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.181"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            198..890
                     /locus_tag="VXD58_002119"
     CDS             198..890
                     /locus_tag="VXD58_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV0676730.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            944..1333
                     /locus_tag="VXD58_002120"
     CDS             944..1333
                     /locus_tag="VXD58_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV0676731.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT          493 a          200 c          298 g          361 t
ORIGIN      
        1 ttagttcaat aatcaatata tcattctaag tctattttat atataaagta tattgtttaa
       61 cgtttgatag aaaagatgct atactagaag taggatgtaa tggcaaaatc taatactaat
      121 aagcgctatg caatacgtaa actcggaaag atttttggtt ctgcggtaat cgggctaact
      181 ttatttttag gaggaatctg aatacaagtt ttgttcatgc tgctgatggg attcaatatg
      241 tcagagatga tactagagat aaagaagagg gaatagagta tgatgacgct gacaatgggg
      301 atattattgt aaaagtagcg actaaaccta aggtagtaac caagaaaatt tcaagtacgc
      361 gaattcgtta tgaaaaagat gaaacaaaag accgtagtga aaatcctgtt acaattgatg
      421 gagaggatgg ctatgtaact acgacaagga cctacgatgt taatccagag actggtcatg
      481 ttaccgaaca ggttactgtt gatagaaaag aagccacgga tacagttatc aaagttccag
      541 ctaaaagcaa ggttgaagaa gtttttgttc catttgctac taaatatgaa gcagacaatg
      601 acctttctgc aggacaggag caagagatta ctctaggaaa gaatgggaaa acagttacaa
      661 cgataactta tgatgtagat ggaaagagtg gacaagtaac tgagagtact ttaagtcaaa
      721 aagaagactc tcaaacaaga gttgttaaaa aaggaaccaa gccccaagtt cttgtccaag
      781 aaatttcaat cgaaacagaa tatctcgatg gcccaactct tgataaaagt caagaagtag
      841 aagaagtagg agaaattggt aaattactct tactacaatc tatactgtag atgaacgtga
      901 tggaacaatt gaagaaacta cttctcgtca aattactaaa gagatggtaa aaagacgtat
      961 aaggagaggg acgagagaac ctgaaaaagt tgttgttcct gagcaatcat ctattccttc
     1021 gtatcctgta tctgttacat ctaaccaagg aacagatgta gcagtagaac cagctaaagc
     1081 agttgctcca acaacaggct ggaaacaaga aaatggtatg tggtattttt ataatactga
     1141 tggttccatg gcaacaggtt gggtacaagt taatggttca tggtactacc tcaacagcaa
     1201 cggttctatg aaagtcaatc aatggttcca agttggtggt aaatggtatt atgtaaatac
     1261 atcgggtgag ttagcggtca atacaagtat agatggctat agagtcaatg ataatggtga
     1321 atgggtgcgt taacagattc ctccctgcga ca
//
LOCUS       DAVLFB010000182         1337 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLFB010000182 DAVLFB010000000
VERSION     DAVLFB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.182"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            144..1295
                     /gene="nagA"
                     /locus_tag="VXD58_002121"
     CDS             144..1295
                     /gene="nagA"
                     /locus_tag="VXD58_002121"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV0676732.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
BASE COUNT          414 a          237 c          325 g          361 t
ORIGIN      
        1 aaaaagagaa gagttggaga aatccagctc ttttaaaata tctccaaaaa ataggtctat
       61 accatttaca aataaaaaag aaaggtttat aatgtaattg acataataaa ttgtagaatc
      121 aatcttttaa ggaggttaac attatgccta actatattaa agcggatcag tttttctacc
      181 cacacggagt tcgtcgaggt ggttacttgg aacttgtgga cggcaagttt gggaaacatg
      241 tagagcagat tcctgaaggg gctgaggtga ttgactatac aggttatagc attgccccag
      301 gtcttgtgga tactcatatt catggatatg caggtgtaga tgtgatggac aacaacattg
      361 aaggtacatt gcatactatg agtgaaggac ttcttagtac cggtgttacc agtttcttac
      421 ccacaacttt aacagccact tatgagcaat tgcttgcagt cactgaaaat cttggaaacc
      481 attataaaga agcaacaggt gctaagattc gtgggattta ttatgaaggt ccatatttca
      541 cagaaacttt taagggggca caaaatccaa cttatatgag agacccgggt gttgaggagt
      601 ttcattcttg gcaaaaagcg gcaaatggct tgcttaataa aattgccctt gcaccagaac
      661 gtgatggggt ggaagacttt gttcgtacag ttacgggcga aggtgtgacg gttgctcttg
      721 gacattcaaa cgcgactttt gatgaagcca aaaaagcagt cgatgctgga gcgagtgttt
      781 gggtgcatgc ctataatgga atgcgtgggt tgactcaccg tgaattgggt atggttggag
      841 ccatgtacca attgccacat acctatgcag agttgatctg tgatggtcac cacgtagatc
      901 caaaggcttg cgaaattctt atcaaacaaa aaggaacaga aaacatcgct cttatcacag
      961 actgtatgac agctggggga ttggaagacg gagattatat gttgggagaa ttcccagttg
     1021 tcgttgcaaa tggaactgca cgcctcaaat cgacaggtaa cttggcaggt tctatcctca
     1081 aactcaaaga tggtttgaag aatgtggtcg aatggggaat tgcgaatccg catgaagcag
     1141 tcatgatggc cagcttcaac ccagctaaat ccgttcacat cgatgacgtc tgtggccaaa
     1201 tccgtgaagg ctacgacgct gacttcatcg tattagataa agatttggaa ttggtagcaa
     1261 cctatctaga tggcgtaaaa cgttatcaag cataagagag aaggcagaag ttagagacta
     1321 gcttctgctt ttttata
//
LOCUS       DAVLFB010000183         1334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLFB010000183 DAVLFB010000000
VERSION     DAVLFB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.183"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(22..1262)
                     /locus_tag="VXD58_002122"
     CDS             complement(join(22..923,923..1262))
                     /locus_tag="VXD58_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV0676733.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
BASE COUNT          344 a          296 c          224 g          470 t
ORIGIN      
        1 ccaaaaaatt agacaaataa tttatccaaa ggatttagtt ctgtactgca cagaactaag
       61 tccttttagc tttaccttaa ttcgtttgtt gttgtagtaa tcaatgtagt ccacaatagc
      121 ttgttcaagg ttttctaaag atctaaagtt cttctcataa ccataaaaca tctccgattt
      181 caaaatgcca aagaaagatt ccatcatgcc gttgtctggg ctgttgccct tgcgtgacat
      241 ggatgcttga attcccttac tctctaggaa ccgatgataa gaatcgtgtt ggtattgcca
      301 gccttggtca ctatggagaa tcgtattctc atagtatttc tctgtaaagg cttgttctaa
      361 catactcttc acttgttcta agttgggcga agtagaaaga tgataagcaa tgatttcact
      421 gttaaaacca tctaaaactg gtgataaata gagtttctgt gtgctatttg gaatggcaaa
      481 ctctgtcaca tctgtatagc acttttccat tggcctcgat gcttcgaact ggcgttgaat
      541 gagattctct gctttcttgc caatctctcc ttggtaggaa gaatacttcc gtttacggcg
      601 aattcgagcc gttaaaccaa gaattctcat caaccgttga actttcttat gattgaccac
      661 aaaaccacga ttccttagtt ctaaatggat tcgacgatag ccatagttgc ctttatgttc
      721 attatcaatg ccctgaattt cggttttaat ctctttatct ttatcaaccc catccagttg
      781 tttcaactga taatagtaag tcgagcgagc taaacgtgct gtttcaagaa gaaaatctag
      841 tcgaaatcct cctgaaacca tttctctaac tgtctctgcc tttctcgctc tagggcttcg
      901 tccctttcct ctaactcttt taactttttt aggtaggcca cctcagtccg taagcgttca
      961 ttctcctcct ggagtcgttc tagttctgtc atttcttccc aagtcttctt ccgtttacgt
     1021 cccattttag ctggtctccc tcttgttttc tcaacaatag tatacccgtt tttcttgtat
     1081 tgtgccggcc aaaatgaaag cattccttgg tttgggaggg cataatcaag actaatactt
     1141 ctttgtgaac aaccttccag taaggcttta tccatcattt cttgtttcaa tttagaagaa
     1201 tagtgacgat tcttcccttt tttaacgatt tctatgccat aacggtcaat taatttcacc
     1261 atgtacttta gaccagaaac atccacacca aatctttttg aaagctgttt gaagctttgt
     1321 ccttgctttc ttag
//
LOCUS       DAVLFB010000184         1331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLFB010000184 DAVLFB010000000
VERSION     DAVLFB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.184"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1331
                     /locus_tag="VXD58_002123"
     CDS             <1..>1331
                     /locus_tag="VXD58_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEV0676734.1"
                     /translation="QSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQIS
                     NLSNRINSNKQGTDNQISNLKTQVATNKDNAERQMGRISDQVSANKANADSQFANVTN
                     QLVRKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEVAKNASNGQ
                     NLLKGTKDFSGGWKNKGANWKKHAEKYKGVDVLFKNNSWNGVGQEIDAKIGEVYTFSL
                     WMKSDWKNDTVNFYVNRNGSVEKGWGVPSETSVAITSEWKRYSFTFKITVDGFIFPRV
                     ERLNQNTNLYIAGLKLEKGSYATPYTEAPEDTDEAIRSVQSQLTGSWAVQNINSAGDI
                     ISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKTANFEAGSVTTTILDAE
                     AVTAEKLKVDDALIRKLTAKAAFIDRLTSERIFSTKVESVISSSTFLEAYQG"
BASE COUNT          445 a          245 c          298 g          343 t
ORIGIN      
        1 caatctgtag atggtaagtt cacggatatt tcaagtcaga taactactta taagcaagat
       61 gtgggcggtc aaatcagtgg tctatcaaat agacttacaa gcagtgagca aggaaccact
      121 actcagattt caaatctttc aaatcggata aacagtaata agcaaggcac agataatcag
      181 atttcaaatt taaagactca ggttgctaca aacaaggata atgctgaacg acaaatgggt
      241 agaatatctg atcaggtttc tgcaaacaaa gcgaatgctg atagtcaatt tgcgaatgtg
      301 accaatcaac tagtgcgaaa agtagagact actgacttcc agcgtgttaa ggaaaccagt
      361 aaactttacg agcggatttt gggcaatact gaaaatggaa ttgcggataa ggttgctcgc
      421 atggctctga ctaatcaact gtttcaggtt gaggtggcta aaaatgccag caatggtcaa
      481 aatttgttga aaggcacaaa agacttctca gggggttgga aaaacaaggg tgcaaattgg
      541 aaaaagcacg ctgaaaaata caaaggtgtt gatgtcctat ttaaaaataa ttcgtggaat
      601 ggcgttggac aagagattga tgcaaaaatt ggtgaagtct atacattcag cttatggatg
      661 aagagcgact ggaagaatga tacagtaaat ttctatgtaa atagaaatgg atctgttgag
      721 aaaggttggg gtgttccatc tgaaacatcg gtcgctataa caagtgaatg gaaacgttac
      781 tcatttacct tcaaaattac tgtggacggc ttcatctttc ctcgtgtaga acgacttaat
      841 caaaatacaa atctatatat tgcaggtctt aaacttgaaa aaggatcgta tgcaacaccg
      901 tacaccgaag ctcctgaaga cacggacgaa gcgattcgct ctgttcaaag ccaactaact
      961 ggctcatggg cagttcaaaa catcaactcg gctggagata tcatctctgg aatcaatctt
     1021 ggcgccaatg gacataaccg ctttgttggg aaattgaccc acatcactgg agagaccctg
     1081 attgacagag cagtcatcaa gtctgccatg gttgataagc tcaaaacggc caattttgaa
     1141 gctggttcgg tcacgactac gatattagac gctgaagcgg taactgctga gaagttgaaa
     1201 gttgacgatg cgcttattag aaaattaact gcaaaagctg cttttattga ccgactgaca
     1261 tctgaacgta tcttctctac taaggttgag tccgtcattt ctagctcaac cttcctagaa
     1321 gcctatcaag g
//
LOCUS       DAVLFB010000185         1212 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLFB010000185 DAVLFB010000000
VERSION     DAVLFB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.185"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1212
                     /locus_tag="VXD58_002124"
     CDS             <1..>1212
                     /locus_tag="VXD58_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV0676735.1"
                     /translation="RKSIFVFNEHYVDQKVKIAQEGLDTIVILGEQVDIDEELDRLRT
                     QLSESQIESQDYYAEYEEYLDEKNEKSPDFWKKEMTDSLKGVGNWAERDREIKGNRAA
                     SPVHNNTFQNFVDLQPILDKNELEVEFNNKKARYFSIRDSAVTINNELSLPDINFDSN
                     ELSTLLSEKIEEPELNSRDKYLLTLLSDSTKGERHLREVKDFFEDEHQKKCPFCTQSV
                     SEDVKVELTNGITKLLSRAVEEHQSALRGKKIDEINQDFSGYEQIDPILIQSYQNSIN
                     ALNAKFNEINSIIDKKIDNPYNIVELPNISFSQELSQAEHDIEKINQAIIKHNSEISG
                     IQKLKVDLLQINNELAFYEIQDAYKKFQEKTNKKAICENNYNNSSKRVKDYEKQISDL
                     EDKKLNIDIAV"
BASE COUNT          501 a          150 c          203 g          358 t
ORIGIN      
        1 aacgaaaatc gatatttgtt ttcaatgaac actatgttga tcagaaagtc aaaattgctc
       61 aagaagggtt agatacaatt gtaattttag gtgaacaggt tgatatcgat gaggaactgg
      121 atagattaag aacgcagtta tcagaatcac aaattgaatc tcaagattat tatgctgaat
      181 atgaagaata tttagatgaa aaaaatgaaa aatctccaga tttttggaaa aaggaaatga
      241 cagattcttt gaaaggagta ggcaactggg ccgaaagaga tagggagata aaaggaaatc
      301 gagctgcatc tcccgttcat aataatacct ttcaaaattt tgttgattta caaccaatct
      361 tagataaaaa tgagttagag gttgaattca acaataaaaa agctagatat ttttcaataa
      421 gagattccgc agttactata aataatgaac tatctttacc tgatattaat tttgatagca
      481 atgaattatc aactttattg tctgaaaaaa tcgaagaacc tgagttgaat tctagggata
      541 agtatttact gacattatta tctgattcca caaaaggtga acgtcatttg agagaagtta
      601 aagatttctt tgaagatgaa catcaaaaaa aatgtccttt ctgtactcaa tcagtatcag
      661 aagatgttaa agttgaactc acgaatggca taactaaact actatcaaga gcagtcgaag
      721 aacaccaatc cgcattgaga ggtaaaaaaa ttgatgagat taatcaagat ttttctggtt
      781 atgaacagat agatccaata ttaattcagt cttatcaaaa tagtattaat gctttgaatg
      841 ctaaatttaa cgaaattaac tcaataatcg ataagaagat tgataatcca tataatatag
      901 ttgagttacc taatataagc ttttctcaag aattaagtca agcagagcat gatattgaaa
      961 aaattaacca agcaattatc aaacataatt cagaaatctc gggtattcaa aagttaaaag
     1021 tagatctcct tcaaataaat aatgaattag ctttttatga aattcaagat gcatacaaaa
     1081 agtttcaaga gaaaacaaat aaaaaagcaa tttgtgaaaa taattataat aacagtagta
     1141 aacgagtaaa ggattatgag aagcagattt ctgatttaga agacaagaaa cttaatatag
     1201 atattgcagt tg
//
LOCUS       DAVLFB010000186         1210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLFB010000186 DAVLFB010000000
VERSION     DAVLFB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.186"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            238..837
                     /locus_tag="VXD58_002125"
     CDS             238..837
                     /locus_tag="VXD58_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676736.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYTTIDKTSDATALLKLKLNNVDNQPATEVLSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNYP
                     VGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            980..1150
                     /locus_tag="VXD58_002126"
                     /pseudo
     CDS             980..1150
                     /locus_tag="VXD58_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          437 a          176 c          245 g          352 t
ORIGIN      
        1 ttaatatttg gagaagagga tggccatctt ctccattttt atgtgcagag ttatagtgac
       61 ttgatgctgg gaaagtacac tgtgactgct aatactcaat gaaaatcaaa gtgcaaacta
      121 ggaagctagc cgcaggctgt acttgagtac gtcaaggtga agctgacgtg gtttgaattt
      181 gattttcgaa gagtataaaa cattcctaga agttgctttg atttccctaa tctatttatg
      241 caaattttat gttattttac tataacagtt gttgctaagc caaataatag tggggaagtt
      301 catttagacg taagcattga agataatcag ggaggtagtg ggcataattt cagttctgtt
      361 tcaagtagct cacaaacagc taaatatgaa gaaactggtt ataataacaa tttatcatta
      421 tatacaacga ttgataaaac gtctgatgca acagctcttt tgaaattaaa gttgaataat
      481 gttgataatc aacctgctac tgaagttctt agttcaggaa ttactgtaaa attaaatgct
      541 aaagataatg ctggaaactg gacaagtgct tcgaataaaa aagaaataac agtaaaaatt
      601 gtttctgcta aaccgacata tccagacaaa atcttagtga aaaatcctga taatataaaa
      661 gatacagaaa aaaatgccat tattgaaaaa ttgaaagagg caaataaaaa ttatccagta
      721 ggagctccaa cctttgctaa aggtgaagga gagcatgcaa atgatattgt agcaacttat
      781 tcagatggta caacttatta tgtaccgtta aatgatgtga caaaatatgc gaggtagtgg
      841 ctgtaccact cacttattca cctccccgtg atttgtagta gtgataggct ttctaactat
      901 tattataaaa caaaataaag agcacaacac tttttcattc tgtgttgtgc cttgagtgaa
      961 acgaaaggaa tgaattataa atatgaaaag tatagtcact agcatagatg agcgcttgcg
     1021 tactcgccta cgagtgatta tctggaagca atggaagaag aaatcgagac gattatgggg
     1081 attgcttaag ttaggggttc ctaaatggat agcagataag gtatctggct ggggcgacca
     1141 ctatcaatta tagtaaaatg aaataagagt aggacgaatc aatcaggaca gtcaaatcga
     1201 tttctaacaa
//
LOCUS       DAVLFB010000187         1206 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLFB010000187 DAVLFB010000000
VERSION     DAVLFB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.187"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..918
                     /locus_tag="VXD58_002127"
     CDS             <1..918
                     /locus_tag="VXD58_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676737.1"
                     /translation="EINSLYLIGYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEI
                     QNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKT
                     TILKLISRLYDYDKGQILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGK
                     QDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAP
                     ILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVES
                     EGKHEELLQKSKIYKNLIEKTKMAEEFIY"
     gene            929..>1206
                     /locus_tag="VXD58_002128"
     CDS             929..>1206
                     /locus_tag="VXD58_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV0676738.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIV"
BASE COUNT          454 a          151 c          242 g          359 t
ORIGIN      
        1 gagataaatt ctctctacct tataggatat ttactagctg ctatgaagat aaaagactct
       61 ttagatgcat ctaaagaggg cttgatggaa atattttatt tatcgcccaa aatagaaaga
      121 ttaaaagaaa ttcaaaatca agatttacaa gaaggcgatg actatagctt aaaaaaattt
      181 gatattgatc taaaagatgt tgagtttgcc tacaataaag acgcaaaagt tttaaatggt
      241 gtaagtttta aagctaagca gggagaggtc actgctttgg taggtgcaag tggctgcggt
      301 aaaacaacta tcttgaaact tatatcaaga ctttatgatt atgacaaggg acaaatctta
      361 atcgatggca aagatataaa agaaatatca acagaatctc tttttgataa ggtctctata
      421 gttttccaag atgtggttct ctttaatcaa agcattatgg aaaatattag aatcggaaag
      481 caagatgcaa gtgacgaaga ggttaaaaga gcagcaaaac ttgcaaattg cacagatttt
      541 atagaaaaaa tggataaagg tttcgataca gttattggtg agaacggagc tgagctatca
      601 ggaggagaaa gacaaagatt atcaatagcc agagccttct taaaagatgc gccgatattg
      661 atcttagatg agataacagc aagccttgat gttaacaacg agaaaaagat tcaagagtct
      721 ttaaataatt tagttaaaga taaaactgtt gtaatcattt cacatagaat gaaatccata
      781 gaaaatgcag acaagatagt agttcttcaa aacggaagag tagaaagcga aggtaagcat
      841 gaagagcttt tacaaaaatc aaaaatttac aaaaatttaa tagaaaagac aaaaatggca
      901 gaagaattta tttattagga ggactacaat ggataataaa aaattaaaag taaaagattt
      961 agtaagtatc ggtgtttttg gcgtaattta ttttgccttc atgtttggag ttggtatgat
     1021 gggcttgatt ccaatattgt tcttaatata cccgacagta ttagccatag ttgcaggaac
     1081 tgttgttatg ttatttatgg ctaaggttca aaagccatgg gcactattta tatttggtat
     1141 gatatcacca cttgtgatgt ttgcagctgg tcatacctac gtagttgtgg ttttatcact
     1201 tatagt
//
LOCUS       DAVLFB010000188         1204 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLFB010000188 DAVLFB010000000
VERSION     DAVLFB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.188"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            162..785
                     /locus_tag="VXD58_002129"
     CDS             162..785
                     /locus_tag="VXD58_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676739.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPCKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            782..>1204
                     /locus_tag="VXD58_002130"
     CDS             782..>1204
                     /locus_tag="VXD58_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676740.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKE"
BASE COUNT          449 a          151 c          236 g          368 t
ORIGIN      
        1 atgactatta caatggttat tctatttaca tttcttatga cagggcaaat agagttgcta
       61 tatattggag cggcttctct gctagttttg atgttgtcaa tcataatgag ttttagaaaa
      121 ttatctgaaa gctacactaa aattttaaaa ggagatgaat catgacaatt gaccttttga
      181 acgtttcaaa aagtttcggc tcaaagaaga tctttacaga cttaaattta atatttgaat
      241 ctggaaaaag ctatgcattg attggaggtt ctggctccgg taaaagtacc cttcttaata
      301 ttataggaag attagaaaaa attgacagtg ggaatgtttt agttgataaa caagatattt
      361 ggaagataaa agaaagaact ttttttaaaa atactgttgg atatgtattt cagaattatt
      421 ctttaataga taacaaaaca gtgtatgata atctgagcct tatcactaaa gacaaaaaaa
      481 caataactga cgtacttgaa aaagtaggac tgtcaagtga ctatttacac caaaaaatat
      541 atgaattgtc tggagggcag gctcaacgtg tagctattgc cagaatgtta atgaaaccat
      601 gtaaaattat cttagcagat gaacctacag gagctttaga tggtgagatt ggaaaagaaa
      661 ttattcgttt actattgaat gaaacagctg aagataaata tgtcattata gcgacacatg
      721 atccagctgt ctataacgaa gttgatgtga taattgacat gaaagatata gggtataagg
      781 tatgaaaaag aaaatttata ttgctttgat ctttgtaact ggggtactcg ccattttctt
      841 ttttggaaaa caaatgatta caaaagaaaa tatcaataag ccaacggtag agttaacaat
      901 ctatacgctt tcttctagtg atacagaaaa atggaataaa gtgagacaag tagaaacgga
      961 agaagctata tacttcataa ctgtgaaaga agtatcatct tcagaagaag tattttcaaa
     1021 catcattgca aatggagctg ctacggggtt cggagttcgt gaagaagaag tgaagaaatt
     1081 caataatggt ttaggagaca ccatagagga ctctaagcat aataaactaa ttgaaataga
     1141 attttttact ttttctgatg atggcgcggg atttgtggta gcaaactttg actacggtaa
     1201 agaa
//
LOCUS       DAVLFB010000189         1181 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLFB010000189 DAVLFB010000000
VERSION     DAVLFB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.189"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..694
                     /locus_tag="VXD58_002131"
     CDS             <1..694
                     /locus_tag="VXD58_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676741.1"
                     /translation="SFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIA
                     ISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDK
                     FPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAF
                     KNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNS
                     ANEWRVSNERVL"
     gene            660..>1181
                     /locus_tag="VXD58_002132"
     CDS             660..>1181
                     /locus_tag="VXD58_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676742.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSIL"
BASE COUNT          431 a          169 c          224 g          357 t
ORIGIN      
        1 atcctttaat ttagaagaag gaaagtccta cgcacttgtc ggttcatctg gatcaggcaa
       61 atcaacagta gcaaaactta tatcaggttt ttacaatgtt aataaaggaa gcataaagat
      121 aggcgggata gcaataagtg aatattctga cgaagcctta attaaagcca tttcctttgt
      181 ttttcaagat tcaaaattat tcaagaagag catttatgat aatgtagctt tagctaataa
      241 agatgcgacg aaagatgacg ttatgagagc cttaaaatta gcaggatgcg atttaatatt
      301 agacaaattc ccagaaagag aaaatacaat cataggctca aaaggtgttt atttatccgg
      361 tggagaaaaa caaagaattg caattgctag agcaatttta aaggattcca aaattattat
      421 tatggatgaa gcatcagcat ctattgaccc agataacgag tttgaattgc aaaaagcttt
      481 taaaaatctt atgaaggata aaacagttat catgattgca cacaggctat ctacaattaa
      541 agaccttgat gaaattattg tcatggatag tggaaaaatt atagaaagag ggtctgacaa
      601 agaattaatg tcaaaagata caaggtataa gagcctgcaa gagatgttta acagtgcgaa
      661 tgaatggagg gtttcaaatg aaagagtttt ataaaaaaag atttgctctt acagatggag
      721 gagcaagaaa tttaagtaaa gcaacactgg cttcattttt cgtttattgt ataaacatgc
      781 ttcctgccat attacttatg atttttgctc aggaagtttt ggaaaatatg ggcaaaagca
      841 atggctttta tatagtattc tcagttttga ctttgatagc aatgtatatt ttgctttcta
      901 tcgaatacga taaattatat aacacaacct atcaagaaag tgcagattta agaataagga
      961 cagcggagaa tttatcaaaa ttacctctat cttacttttc taaacatgac atttccgaca
     1021 tttcacaaac aatcatggct gatattgaag gcatagagca tgcaatgagc cactcaatac
     1081 caaaggtggg cggcatggta ctgtttttcc cattaatatc tgtaatgatg ctagcgggca
     1141 atgtcaagat gggtttagct gtaattattc catctatttt a
//
LOCUS       DAVLFB010000190         1164 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLFB010000190 DAVLFB010000000
VERSION     DAVLFB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.190"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..663)
                     /locus_tag="VXD58_002133"
     CDS             complement(<1..663)
                     /locus_tag="VXD58_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676743.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIF"
     gene            complement(673..>1164)
                     /locus_tag="VXD58_002134"
     CDS             complement(673..>1164)
                     /locus_tag="VXD58_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676744.1"
                     /translation="LKSIKHFTSFSFKLAALSTGIWTATLFLLIFLIAFSNGFSFSLE
                     IKEVDFLREFYGISIANNASFFIGFFFSYIAYYFFLSLLTISSFSWFKKSNMSLVFLF
                     TFLFVESLFWIYQLDNGIIGLLPIFQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNW
                     RRV"
BASE COUNT          501 a          218 c          136 g          309 t
ORIGIN      
        1 gaaaatactt ttaaaaagaa agactaaaaa tagcaactga agaaataaat ctcgtcctat
       61 atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc ctacaaatag
      121 accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca tttgtacatt
      181 cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat tcaaagaatc
      241 aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt gaatcactag
      301 aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct gatacctaat
      361 ccagagctta atagaagaaa atcgaatcac ttccataaag aaatcggatg ctcttctacg
      421 atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc atatagataa
      481 gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa caagtctatc
      541 atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa gaacataacc
      601 ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac ttaactttcc
      661 catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag tcaatggaat
      721 tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat ttaccatata
      781 ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc agaataagga
      841 ttctacaaat aaaaaagtaa acagaaatac taagctcatg tttgattttt taaaccaaga
      901 aaaactgcta atagtaagta aggataaaaa gaaatagtat gctatataag agaaaaaaaa
      961 tcctataaag aaactagcat tgtttgcaat acttatacca taaaattctc ttaaaaaatc
     1021 aacctccttt atctccaaag agaagctaaa accattacta aatgcaatta gaaaaatcaa
     1081 taaaaataaa gtcgccgtcc aaatccccgt actaagagct gctaatttga aactaaaact
     1141 ggtaaagtgc ttaattgatt tcag
//
LOCUS       DAVLFB010000191         1134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLFB010000191 DAVLFB010000000
VERSION     DAVLFB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.191"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1134
                     /gene="pcpA"
                     /locus_tag="VXD58_002135"
     CDS             <1..>1134
                     /gene="pcpA"
                     /locus_tag="VXD58_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV0676745.1"
                     /translation="SLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKY
                     KVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYY
                     PDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELE
                     EIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKT
                     LGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETK
                     ELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLE
                     LKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSG"
BASE COUNT          429 a          167 c          186 g          352 t
ORIGIN      
        1 tatcattaac tacagctgca gttattttag cagcatatgt ccctaatgaa ccaatcctag
       61 cagatactcc tagttcggaa gtaatcaaag agactaaagt tggaagtatt attcaacaaa
      121 ataatatcaa atataaggtt ctaactgtag aaggtaacat aggcactgtt caagtgggta
      181 atggagttac tcctgtagag tttgaagctg gtcaagatgg aaaaccattc acgattccta
      241 caaaaatcac agtaggtgat aaagtattta ccgttactga agtagctagt caagctttta
      301 gttattatcc agatgaaaca ggtagaattg tctactatcc tagctctatt actatcccat
      361 caagcataaa aaaaatacaa aaaaaaggct tccatggaag taaagctaaa actattattt
      421 ttgacaaagg tagtcagctg gagaaaattg aagatagagc ttttgatttt tctgaattag
      481 aagagattga attgcctgca tctctagaat atattggaac aagtgcattt tcttttagtc
      541 aaaaattgaa aaagctaacc ttttcctcaa gttcaaaatt agaattaata tcacatgagg
      601 cttttgctaa tttatcaaat ttagagaaac taacattacc aaaatcggtt aaaacattag
      661 gaagtaatct atttagactc actactagct taaaacatgt tgatgttgaa gaaggaaatg
      721 aatcgtttgc ctcagttgat ggtgttttgt tttcaaaaga taaaacccaa ttaatttatt
      781 atccaagtca aaaaaatgac gaaagttata aaacgcctaa ggagacaaaa gaacttgcat
      841 catattcgtt taataaaaat tcttacttga aaaaactcga attgaatgaa ggtttagaaa
      901 aaattggtac ttttgcattt gcagatgcga ttaaacttga agaaattagc ttaccaaata
      961 gtttagaaac tattgaacgt ttagcctttt acggtaattt agaattaaaa gaacttatat
     1021 taccagataa tgttaaaaat tttggtaaac acgttatgaa cggtttacca aaattaaaaa
     1081 gtttaacaat tggtaataat atcaactcat tgccgtcctt cttcctaagt ggcg
//
LOCUS       DAVLFB010000192         1106 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLFB010000192 DAVLFB010000000
VERSION     DAVLFB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.192"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>1106
                     /locus_tag="VXD58_002136"
     CDS             <1..>1106
                     /locus_tag="VXD58_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV0676746.1"
                     /translation="NSLIQHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSNP
                     NGTFRLGATMDAREVELPDGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGA
                     TVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTI
                     SNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGI
                     VGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVT
                     NGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKK
                     ATFVSKNTIKTEDFNFSSRYVTDYKNLENADSSK"
BASE COUNT          424 a          142 c          217 g          323 t
ORIGIN      
        1 aatagtctga tacaacatga aaataataat gtactagaaa attatacata ctatttacca
       61 aaaactcagc aaagtgaaac tggggtatat acatcattta aaaatttagt agatgcaatg
      121 aatagcaatc ctaatggcac attccgttta ggagcaacta tggatgctcg tgaagttgaa
      181 cttccagatg gtcaagaaag ttatgtgaat aatgtattcc atggaatatt agtaggaaca
      241 aataatgaga aatattatgc tatttataat ttgaaaaaac cattatttgg ggaattaaat
      301 ggtgctacag tagaaaaact ttcactaaaa gatgttaata tttccgcaaa ggacgatact
      361 gcaacacttg cgaaagaagc aaataacaat actcatattg ataacgttca tgctgatgga
      421 gcgatagctg gggaacgtag cataggagga ttagtatcac aagttaataa ttctactatt
      481 tctaacagta gctatacagg tagaataact aatacatata aaacagttgc tagttatcaa
      541 atcggaggtt tagtaggcaa actttctggt ccgaggggat taatagataa gtcgttcgca
      601 tcaatcgatt tatcttcaaa tgcaacacaa ggagaccagt ctattggtgg aatagttggt
      661 gcagtagaaa atagtgcgct tattagtaat agttatgctg aaggaaatct aaataatgtt
      721 caacgttttg caaacgttgg tggagtagtt ggtaatcttt gggatccggt tggaggatta
      781 gaaaaatccg gtcgactttc taatgtacta agcgatgtta atgtaacgaa tggtaatgct
      841 attgcaggtt ataattttaa tggtataaaa gcaaatggca catacagtaa taaaaataat
      901 aaggttgtga acgtagtaca agaagatgat gaaatcttaa ctaaggattc tactgttcaa
      961 agaggagaag tattagaaga tgcacaaata aaagagaaaa aagctacttt tgtatctaaa
     1021 aatactatta aaacagaaga ttttaatttc tcttctagat atgtaactga ctataaaaat
     1081 cttgagaatg ctgattcatc aaaaga
//
LOCUS       DAVLFB010000193         1101 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLFB010000193 DAVLFB010000000
VERSION     DAVLFB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.193"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..796
                     /locus_tag="VXD58_002137"
                     /pseudo
     CDS             1..796
                     /locus_tag="VXD58_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(831..>1101)
                     /locus_tag="VXD58_002138"
     CDS             complement(831..>1101)
                     /locus_tag="VXD58_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV0676747.1"
                     /translation="FFEAWFQKFLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKL
                     LPLPPYSPEYNPIEKTWAYIKKNLKKVLPSCNTFYEALFSCSCFN"
BASE COUNT          364 a          224 c          186 g          327 t
ORIGIN      
        1 tagaattctt cttagaaaag gccgttttaa gtgctatcat tgctcaaaaa tggagctcgc
       61 tgagacttct atcgtcaaga agaatcatca aattccttgt atcatcaacc aaaagattgc
      121 tcaaaagtta attgaaaaga tttctatgac cgacattgat catcagttgt ctatttcaac
      181 ttcaactgtc attcgcaaga tcaataattt tcactttgag catgattttt cgcgtcttcc
      241 tgagattatg tcctaggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg
      301 gagatgagct ttattgctca ggactttgat aaactcaata tcataactgt tcttgagagc
      361 agaacacaag ccatcatccg aaatcccatg aatacaaggc tatcaagcgc tactggaagc
      421 tcattcaaca agatagtcag aaactgagtg ataaacgatt ttatcgccct acttttcgta
      481 tgcacttgac taataaagaa attctagata aacttttgag ctattccgaa gacttgaaac
      541 accactataa tctctatcag ctcttgcttt tccacctctg tttcagactt tgtttaaaac
      601 cttcctcaaa gataaagaga aaatcgtcaa cgcccttcaa ttaccttatt ccaacgccaa
      661 actggaagcc accaataatc tcatcaaact tatcaagcac aatgcctttg gttttaggaa
      721 ctttgaaaac ttcaaaaaag aaaggacgaa atttgtcctt tctagatctt cgctttcttc
      781 aacccactac agttgacaaa gaaccgaaaa atgtctcgcc tctaatatag tcaattgaaa
      841 caagaacaag agaaaagagc ctcgtaaaag gtattgcaac ttggtaatac ctttttgagg
      901 ttctttttga tataagccca tgttttctca ataggattgt actcaggtga gtagggagga
      961 agaggtaaaa gtttatgccc aaactcctcg cataaaagtt ctaacttacc cattctatgg
     1021 aatcttgcat tatccataat aataaccgat ggtgtggtta atgttggtaa gagaaacttc
     1081 tgaaaccaag cttcaaaaaa g
//
LOCUS       DAVLFB010000194         1034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLFB010000194 DAVLFB010000000
VERSION     DAVLFB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.194"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            14..1024
                     /locus_tag="VXD58_002139"
     CDS             14..1024
                     /locus_tag="VXD58_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEV0676748.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
BASE COUNT          356 a          127 c          193 g          358 t
ORIGIN      
        1 tattgcagac tctttggtta ttaataatat ttatgacgaa tatccaattt ggagtatacc
       61 tgttaatttt aaaggttcta aatggtgtct tgaacaaacg ggtaatgata tgtatagggg
      121 tagagctgga atattactgt ttatgtacta tgtaaaatta ttgaaagata aatcatcata
      181 tactcaactg tacaataaaa tgttatctgc aattccgcca tctgtttctt tatctaagac
      241 cacatttggt ttaactgggg atataggata tttcattgtt ctatccatta tagaggatta
      301 cgatacaaat actctcgcat gcttaaatta tataaagagt gttttaactg agcttgaaaa
      361 gactgatttt gcaagtgttt caaacaagtc tgactatatt aatggtcttc tacctttaat
      421 taatacactg gtaagatatt ataggagagg aatagagaag gaacgtgttc taaggctagt
      481 attaagttta ggtgatacgc tatataatga tgtaagcgag agagataata gtaattttgg
      541 atatagtttt ggccatggac tatcaggtgt aatttttaca ttaaaaaata tttataaagt
      601 gacaagactt aaaaaatata aagaattaat tttacaacta gttcctaggg agaaaatcaa
      661 aattcattct ttgaagtggt gtagtggaga atttgggaca ttagttaatc atccccaaag
      721 tgttccagag ggaattgtag ctagtttaga taatgataca ttttgccatg gaagtatggc
      781 cataataaat ttttacatcg actacatgaa agaaatggat actagtatgg aattggagtc
      841 tttattgtgt agtgttgtaa tgaataaatc aaaagaagga gagtatcgta tcgtccaaac
      901 aagcaatttc ccagattttt ctttgtatac aggtgtgact ggtattgctt atactttttt
      961 aaggtatatc gcactgaatc agaatgacaa aaagatagtt atcccctcat tacttgaaat
     1021 ctagcttaat ttat
//
LOCUS       DAVLFB010000195          980 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLFB010000195 DAVLFB010000000
VERSION     DAVLFB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.195"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>980
                     /locus_tag="VXD58_002140"
     CDS             <1..>980
                     /locus_tag="VXD58_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV0676749.1"
                     /translation="GNIEHDKDNLESSIVRKYEWDIDKVTGGGESYKLYSKSNSKVSI
                     AILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIISDIQDRLGHGTAVVAQ
                     IVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDGNDIINLSTGQYLMID
                     GEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSNQSDLLKLISSRKKVR
                     KPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPAGSTLSLSELGLNNFI
                     NAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYKLKDQPYN"
BASE COUNT          360 a          122 c          195 g          303 t
ORIGIN      
        1 ggaaatatag agcatgacaa agataactta gaatcgtcga ttgtaagaaa atatgaatgg
       61 gatatagata aagttactgg tggaggcgaa agttataaat tatattctaa aagtaattct
      121 aaagtttcaa ttgctatttt agattcagga gtcgatttac aaaatactgg attactgaaa
      181 aatctttcaa atcactcaaa aaactatgtc cccaataaag gatatttagg aaaagaggag
      241 ggagaggaag gaataatatc agatattcaa gatagattag gtcatggtac ggctgttgta
      301 gctcaaattg taggggatga caatattaat ggagtaaatc ctcacgttaa tattaacgtc
      361 tatagaatat ttggtaagtc gtcagctagt ccagattgga ttgtaaaagc aatttttgat
      421 gctgtagatg atggcaatga tattatcaat cttagtactg gacaatattt aatgattgat
      481 ggagaatatg aggacggaac aaatgatttt gaaacatttt tgaagtataa aaaggctatt
      541 gattacgcga atcaaaaagg agtaattata gtagctgcat tagggaatga ctccctaaat
      601 gtatcaaatc agtcagattt attgaaactt attagttcac gcaaaaaagt aagaaaacca
      661 ggattagtag ttgatgttcc aagttatttc tcatctacaa tttcggtcgg aggcatagat
      721 cgcttaggta atttatcaga ttttagcaat aaaggggatt ctgatgcaat atatgcgcct
      781 gcaggctcaa cattatctct ttcagaatta ggacttaata actttattaa tgcagaaaaa
      841 tataaagaag attggatttt ttcggcaaca ctaggaggat atacgtatct ttatggaaac
      901 tcatttgctg ctcctaaagt ttctggtgcg attgcaatga ttattgataa atacaaatta
      961 aaagatcagc cctataatta
//
LOCUS       DAVLFB010000196          964 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLFB010000196 DAVLFB010000000
VERSION     DAVLFB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.196"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..102)
                     /locus_tag="VXD58_002141"
     CDS             complement(<1..102)
                     /locus_tag="VXD58_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV0676750.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVK"
     gene            complement(115..864)
                     /locus_tag="VXD58_002142"
     CDS             complement(115..864)
                     /locus_tag="VXD58_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV0676751.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          333 a          190 c          109 g          332 t
ORIGIN      
        1 ttttacagaa tctatatcat atgcaacaac tttatattta gatgcaaata gtaacgcgta
       61 ggccagccca acatgcccca aaccaattac tgctatattc ataaaactac ttccttattt
      121 cttaatccaa aatctaatag aataagctgc cccattcctt aaatacaact ctttaatatt
      181 gtttaaaagt ttttcaactg atttccagat tatcaaaatc tgagatttat agcacaatat
      241 tgatgatatt ctatcaatat aatgtttttc atcaagttcc tcttgataca tttttaattc
      301 tttagttttt cccatataac taaccatact actatcactt acatatggga agtcctcata
      361 atatattact ttataacgca taaattcaag cgcccttcca atactattca caaaaacatg
      421 atcaacatgg tcaccaagtg aaagcggaca atatacgaca catttgtcgt ctaaatgcat
      481 taacagctct tttatgatat cattctttaa tgtgtcctca ttttttaatt cactatagat
      541 atgacggtat agaaaattgc catttctatc tttcctatag agacattcat agtacgataa
      601 gtgtctaaaa tcacactgta gacgttcaca agctaacctg tcttctttct tcctttcttc
      661 aatcggatat ttcccaaggt tacacaactt atgaaattgc ttagcagagg gctgtagctg
      721 ttggctcaaa gggtaaccag aaaatatagt aataacaagt acaatttctc cttctgaagt
      781 taattttgaa atataatcac cacaggaaaa aattgcgtca tctaaatgtg gagataaaaa
      841 gatatactta gtattgttac tcataaccat tccctctaca atttatctaa aaactcacta
      901 agtgtctgat taaattccac atcatcaaaa aaattcacct tattcttaat aatgaatatt
      961 tcgt
//
LOCUS       DAVLFB010000197          939 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLFB010000197 DAVLFB010000000
VERSION     DAVLFB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.197"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..344)
                     /locus_tag="VXD58_002143"
     CDS             complement(<1..344)
                     /locus_tag="VXD58_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676752.1"
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSE
                     LSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITL
                     SFSILLCYLFNPS"
     gene            complement(325..921)
                     /locus_tag="VXD58_002144"
     CDS             complement(325..921)
                     /locus_tag="VXD58_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676753.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
BASE COUNT          353 a          148 c          105 g          333 t
ORIGIN      
        1 gatggattaa ataaataaca aagtaaaatg ctaaaactta aggttatatt tttagataaa
       61 agaatatctc caaagttcct cttattctta tcaaaaaata atgtaatcag aaagagagaa
      121 aatccatatt caataagagt tgctataaat gtgaaaacaa cagaaatcac tgtcaatatt
      181 gtttctattt taattgtaaa aagattagat agttcagata atttatcaat ttgttcatct
      241 aaaattcccg acttcatcag taaatcaata aacaaatata aaagaaaaag ataaataatc
      301 agattcatta atttgtataa actattattt gtttgaaatt tcataaaccc gtccttcctc
      361 tattaaaatc ctttgagatt tgctaatcaa ttcttgattc aataaattat gagatacata
      421 tataatagta gagctttctt tcatggaaat taagcgttta gcaatcatat caatacttct
      481 tgcatccact gttgataaag tctcatcaat aaaaatataa tcccaatttt tagcaaaaat
      541 taaggataag cctaatttga attttgttcc agtagatgct gtcatatagt tatctccgat
      601 tttattatca aaaaaagtat taatttgttc tttatcctca aatgatagat ggatttgaaa
      661 agaatcacac aacaactgca aattttcttc tatagtaaat ggaacaaccc caagctgatt
      721 ggtatcaagg tatagtacat ctttggagac acaattcttt aaaccactat caaaagtctc
      781 tagcccagct aaaagttgta gaagggtgct ctttcctgaa ccattctctc cttcaactct
      841 gtaaattccc ttatcaatct ctagtgatat atttttcaat atacatttat cataactttt
      901 agattcattt tcaattttca taatttaatt ccctctatc
//
LOCUS       DAVLFB010000198          903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLFB010000198 DAVLFB010000000
VERSION     DAVLFB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.198"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            240..>903
                     /locus_tag="VXD58_002145"
     CDS             240..>903
                     /locus_tag="VXD58_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV0676754.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QAL"
BASE COUNT          320 a          159 c          187 g          237 t
ORIGIN      
        1 tagccacctt accttagtca agaattagat gtttcactat gtttgagtaa gttgatgatt
       61 tcatttgata acaggtttga aactgttagg ctaggtggcc aaggctaatc atagccttgg
      121 tttagctgaa aaaacaggtt caagggttcc tgttgtcaaa tgaaatgtga tttaaggtat
      181 aagaaaacac ctctgtgcta tacttgttgt tcaccacaaa cacaaggaaa ggcacagaga
      241 tgcaagaaca ttatacccca aaagggaaac atttgacaat agataaccgt cgcttgattg
      301 agcggtggaa gaatgaaaat aagtccaatc gtgaaattgc aggcttgtta ggaaaggcgc
      361 ctcaaacgat tcatactgaa gtcaaaagag gtacaacttt acaacaagtg agaaaagggc
      421 tatacaaaaa ggtctattct gccgattacg cacaaactgt ttaccaattc aatcgaaaac
      481 ggtcggtgaa aaagttaatt ttaacaaagg aaatcagaga gaagatctta cactatcata
      541 agcaaaaatt ttcgcctgaa atgatggtta acaagaagca agtgaaagtt ggtatttcaa
      601 ccatctacta ctggtttcat aatggtcatt taggattgac gaaggccgac atgctttatc
      661 ccagaaaaag gaaaggtgtc aagaagcaag ctagtccgaa ctttaagccg gcaggtaaat
      721 ctatcgaaga acgtcctgac gttattaatc ttcgcttgga aaatggtcat tatgaaattg
      781 ataccgtcct actgactaag ataaaaaatt attgcctgtt agtcttaacc gaccggcgga
      841 gcagacacca aattataagg ttaattccaa ataaaactgc tgaatctgtc aatcaggcgc
      901 tta
//
LOCUS       DAVLFB010000199          880 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLFB010000199 DAVLFB010000000
VERSION     DAVLFB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.199"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            299..>880
                     /locus_tag="VXD58_002146"
     CDS             299..>880
                     /locus_tag="VXD58_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676755.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTEL"
BASE COUNT          324 a          145 c          117 g          294 t
ORIGIN      
        1 gttattatct cattttacta taatatttga taagttatcc taaaagtatt attatgttgt
       61 tgtgttatag attgattgaa tctaactaaa ggatcctatt caattactag aactatcaca
      121 tactcaaggt cagctcacag atgagcaact attttggtta caatgtctac taaatttaag
      181 tcaaacaaat aatttagtca aaattaaaaa aatagaggaa cataaatatg attacaaaac
      241 agaatgtaat agtgttctac aatttttact agataaaact gtaaattctg aaggaaggat
      301 cacttcttca acagaatttg gaaatttcgt aagtaattta tcattccaac acggaatagc
      361 tggactactg tttcctctaa ataaattgta ccccccagaa ctggattcta aaatactctc
      421 tatcatcaag aaggcagtga caattagaac gacacacaca tatgaatatc aatactcact
      481 gctatttggt gatgcaggct atctatggtt actcctacat ttattttcta tcagtaaaaa
      541 tcaatactat ctacaattag caaacgtcac cgctaaaaaa ttaatagaga attatgatac
      601 tctagaggaa atagactttg cattgggaaa atctggtgtc ctattatcat taataaaata
      661 ctatcaattt accaatgaca atactcttaa aattttcatc cacaatagta taggggaaat
      721 ttatcattat ttcctacaaa gagatacagc caaagaaagc attttagact atagctttgc
      781 tcatggatat tgtggaattg catatgcttt atttgcctat tctaaagtct tagaaccttc
      841 tatgttctat aatgatctcc atacattcca tactgaatta
//
LOCUS       DAVLFB010000200          872 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLFB010000200 DAVLFB010000000
VERSION     DAVLFB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.200"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            72..869
                     /locus_tag="VXD58_002147"
     CDS             72..869
                     /locus_tag="VXD58_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV0676756.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          228 a          150 c          193 g          301 t
ORIGIN      
        1 atacagaaaa cgcttcctaa aacctaaagt ttgtgatata attatcaaga aaaaagattt
       61 aggagtttat tatggtttct tcagaattta tctcaaagat tgaatttgct tgcaataaga
      121 aagaaagtct ttatagtcaa agcaaattta agtatgcgat tcgttcgatg ttcgcaggtg
      181 catttttaac cttcagtact gctgcaggtg cagttggggc tgacttgatt aataaaattg
      241 caccaggtag tggacgcttc ctctttccat tcgtttttgc ttggggcttg gcctacattg
      301 tttttttgaa tgccgagttg gtcacttcaa acatgatgtt cttgactgct ggtagtttct
      361 taaaaaaaat ctcttggaga aaaacagctg agattttact atactgtacc ttgttcaacc
      421 ttatcggagc cttgatagca gggtggggct ttgctcattc ggcagcctat gcgaatctga
      481 tacacgatag tttcatctca ggtgttgttg agatgaagtt aggccgctca aatgaattgg
      541 tcttgcttga ggcgattttg gcaaatattt tcgtaaatat tgcgattctg tcatttattt
      601 tggtcaaaga tggtggtgcc aaactttggc ttgtgttgtc agctatttac atgtttgtat
      661 tcttaacaaa cgagcacatt gcggcgaact ttgcttcttt cgcgattgtg aaattcagtg
      721 ttgctgcgga ttcaattgcc aacttcggtg ttggaaatat gcttcgccac tggggtgtga
      781 ctttcatcgg aaactttatc ggaggaggcc tcttgatggg tcttccatat gccttcctca
      841 ataaaaacga agatacttat gtagattaag aa
//
LOCUS       DAVLFB010000201          864 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLFB010000201 DAVLFB010000000
VERSION     DAVLFB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.201"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            183..452
                     /gene="blpJ"
                     /locus_tag="VXD58_002148"
     CDS             183..452
                     /gene="blpJ"
                     /locus_tag="VXD58_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV0676757.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            521..769
                     /gene="blpK"
                     /locus_tag="VXD58_002149"
     CDS             521..769
                     /gene="blpK"
                     /locus_tag="VXD58_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV0676758.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          274 a          104 c          207 g          279 t
ORIGIN      
        1 agatatttta gatactcaag attattgaaa tcttagacta aaaaataggt actgctacct
       61 ttaagaagat agtagacgta tatacttttt taagaaaatc aaaaagatac taaaaaaaat
      121 ctatttgttt attgaattta agactttggt aacaaattga aaataaaaag gaggtattca
      181 tcatgaatac aaaaatgttg tcacagttag aggttatgga tactgaaatg cttgcgaaag
      241 ttgaaggggg gtatagctcc actgattgtc aaaatgcact gattacagga gtcactacgg
      301 gaattataac tggtggaact ggagcaggtt tggccacttt aggagtagct ggacttgctg
      361 gtgcatttgt tggagcacac attggtgcaa ttgggggtgg attaacatgc ttaggaggta
      421 tggttggtga taaattggga ctaagttggt aagacttata gtaaaattat gaggattcta
      481 ttctgattta ttttagtttt ttaaaaaagg agtatttgtc atggatacaa aaatgatgtc
      541 acaattttct gttatggata ctgaaatgct tgcttgcgtt gaaggtggcg gatgcaattg
      601 gggagatttt gccaaagcag gtgttggagg agcagctgta gttgctgctt taggttgtgc
      661 agcgggtgga gtgaaatatg ggaaaattct aggaccatgg ggcgctgcaa taggaggaat
      721 tggaggagca gtggtttgtg gatatttagc ctataccgct acatcataaa aatgaaagct
      781 aaattttatg aaggctatat tctttattat tctcttcgct tttcaaacct atcttattta
      841 tctatccata agtatctctg ataa
//
LOCUS       DAVLFB010000202          863 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLFB010000202 DAVLFB010000000
VERSION     DAVLFB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.202"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..782)
                     /locus_tag="VXD58_002150"
     CDS             complement(<1..782)
                     /locus_tag="VXD58_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676759.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIE"
BASE COUNT          297 a          167 c          118 g          281 t
ORIGIN      
        1 aattctatat tatagttttt aaaattattg acattaccat gcactaattt gtctttttgc
       61 atatcttcgt aaagcgcctc taaagtatct actgcataat ttccttgaga aatatacatg
      121 gagtaccaca tcattctcat gaatgaaaca aagagaactc ctgataaaaa taaaatcatg
      181 ataagctcaa gtaaaatcac ctttgcgcta gctaagctag tcataaaata aactatagga
      241 ataattaaaa ttgcaatcag tccaaaaaat aaccattgat acaaaacata aggcctttta
      301 caagataggg aataatcata agcatacttt gagtaatctt ttatcgcctt ataaaagctt
      361 ttaaaagact ctacatttgc tttaaatatt tttacaactt gcattcctct cacgtactca
      421 acagtttcag cacttagttt agatagggat tcttggtata tcttcataaa ttcttgctcg
      481 cccatcattg cccctaaaat taagccacca attatagtaa gagcaagcaa aattatgcca
      541 acccttatac ttactataaa gccaagtgca agtacaagta cgggtgtgat tattgcctga
      601 gaactatcgg gaatcatgtg tgctaccacc tgatgagttt gtgctgcatt atcatctatg
      661 atctttctta tttgaccaga tggatttaag tcaaagaacc taaaacttgc tttttccaga
      721 ccatcgattc ccctttttct taaatttgtt tcaagcctaa atcccaagat atgtgaaaac
      781 attcctgaga caaaataaaa tatcgctcca cttgttagtg taataacaga ttttaatgct
      841 atactctctg caccggataa gtt
//
LOCUS       DAVLFB010000203          846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLFB010000203 DAVLFB010000000
VERSION     DAVLFB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.203"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..845
                     /locus_tag="VXD58_002151"
                     /pseudo
     CDS             1..845
                     /locus_tag="VXD58_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          306 a           73 c          184 g          283 t
ORIGIN      
        1 tatgttaaaa aaacttgaca agaatggatt tggaaaggtt aagacagtga taacatgcca
       61 tggatgggtt gaatataact taaaaaagaa attcttgact tattttgatt tttggacata
      121 ttctatggga gatgctttta tttgtgtatc tgaaactatg aaaaaaagat tggagagtat
      181 aataaaaaaa taagaagata gttgtaataa ataacggaat taatgtatca aatagcgatt
      241 tagatgtagt aggtgttcaa gactttaaaa aagaattttg tattccaaat aacaaaaaaa
      301 tcatttgtta tgttggaagg ttggatccag aaaaaaggcc ggatagattc cttgaatttg
      361 cagaaaaatt atttttagtc agagaagatg tgatttttat tatggctgga aatggtagta
      421 tgtgggctgc tttgaaagaa aaaatttgcc atttgaaatg tagagataat ttcaggttac
      481 ttggtgaaat ctatccagct actatagttt accaaatatc ggatttattg tatattcctt
      541 ctgatacaga aggaatacct atgtgtgttt tagagtcaat gtcacaagga actccggttt
      601 tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca tagggttgat ggatttttat
      661 ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa ttttttactc aatgattctg
      721 agtatttgaa atatataggt gagaatagta aatcaaaaat aagaaaacat ttttctgtgc
      781 aaaaaatgtt tgtagaaacc atgagagtat atgatgaatt attagagaag agtagtcatg
      841 gatagg
//
LOCUS       DAVLFB010000204          769 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLFB010000204 DAVLFB010000000
VERSION     DAVLFB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.204"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..668
                     /locus_tag="VXD58_002152"
     CDS             <1..668
                     /locus_tag="VXD58_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676760.1"
                     /translation="TLIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPI
                     IPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQR
                     QRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLT
                     ARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHH
                     VNS"
BASE COUNT          287 a          139 c          123 g          220 t
ORIGIN      
        1 caacactcat taaaaccttg tctcgactta taaagccatt agagggagaa gtattgcttg
       61 ataataaatc aattaattct tataaagaaa aagatttagc aaaacacata gctatattac
      121 ctcaatctcc aataatccct gaatcaataa cagtagctga tcttgtaagc cgtggtcgtt
      181 tcccctacag aaagcctttt aagagtcttg gaaaagatga ccttgaaata ataaacagat
      241 caatggttaa ggccaatgtt gaagatctag caaataacct agttgaagaa ctttctgggg
      301 gtcaaaggca aagagtatgg atagctctag ccctagccca agatacaagt atcctacttt
      361 tagatgagcc aactacttac ttggatatct catatcaaat agaactatta gacctcttga
      421 ctgatctaaa ccaaaaatat aagacaacca tttgcatgat tttgcacgat ataaatctaa
      481 cagcaagata cgctgattac ctatttgcaa ttaaagaagg taaacttgtt gcagagggaa
      541 agcctgaaga tatactaaat gataaactag ttaaagatat ctttaatctt gaagcaaaaa
      601 ttatacgtga ccctatttcc aattcgcctc taatgattcc tattggcaag caccatgtta
      661 actcttaata attgaatatt ggaattcatt taaaagtcgc aaattttgaa ctgaccctca
      721 aaagttaggc caaaaaacta actaaaggag gtcagttttt taatggcaa
//
LOCUS       DAVLFB010000205          758 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLFB010000205 DAVLFB010000000
VERSION     DAVLFB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.205"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>758
                     /locus_tag="VXD58_002153"
     CDS             <1..>758
                     /locus_tag="VXD58_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676761.1"
                     /translation="FLRIEDVFNTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDY
                     VLKGINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLY
                     RKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIA
                     EEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILI
                     AHRLDTIKSADKILVMHNGEIVEIGTHRELLELG"
BASE COUNT          276 a           88 c          147 g          247 t
ORIGIN      
        1 ttttttaaga atagaggatg tgtttaatac taaatccgaa ttaattccag aaagagtcag
       61 tcaagatata aaatttgata aaaaaataga attaaaagat atttggtata aatatggatt
      121 atttgatgat tatgttttga aaggaataaa tgttactatt aaaaaaggag aaactgttgc
      181 tattgttgga gaatcaggtt caggtaagag tacattagct aaaattttat taggtttatt
      241 agaacctaat attggttcaa tagaagttga tggagtagaa aaagaagaaa ttggtcaaac
      301 attgtataga aagatttttg gagcagtgtt acaaaattca accctaagtt atggtacctt
      361 aagagagaat ttgacatttg gacactttgt ttcagatgaa gaattaatga caaatctaaa
      421 ttcaattggt cttagcaatg tagttaaatc tttacctctt ggattagaga caatcatcgc
      481 tgaagaaggt aataactttt ctggagggca gcagcaaatg atacttttag ctcgttgtct
      541 tttgtcgaaa ccttcggtag ttgttttgga cgaagcaaca agtagtttag ataatttatc
      601 tcaacaaatt acaacttctt acttaagtga aatcggtacc actaagattt taattgccca
      661 tcgactagat actatcaagt ctgcagataa gatcttagta atgcataatg gtgaaattgt
      721 agagattggg acccatagag aacttcttga actaggag
//
LOCUS       DAVLFB010000206          740 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLFB010000206 DAVLFB010000000
VERSION     DAVLFB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.206"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            107..403
                     /locus_tag="VXD58_002154"
     CDS             107..403
                     /locus_tag="VXD58_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV0676762.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          250 a           94 c          118 g          278 t
ORIGIN      
        1 aaagtagtat aaaatataga cgaacatttg aagagagtta ggaatacaac atgtgcgaat
       61 taactctcaa taaaacatac ataatataca aaaggagtac actaaaatga aatctaaaag
      121 aatggaattt cataataaat ttcttattgt ttcagcaatg ttagcagtca ttagttggtt
      181 aagcttaggt gttgtttctt ttcctatgtt ggctgggaca ttaggaattt cgacaaaagc
      241 tgctgctact gtagtcaatt tgattagtgc ttatagtaca gttacagcag taatttcaat
      301 tgtaggtgca atcacaggag taggttctat tggttcgggt attgcagcaa cagttcttta
      361 tatattgaaa aagaaaggtg ctgctaaagc agctctatgg tagaactaga tgaaaatact
      421 ttctaaaaaa gaatgtgaaa gtattttagg ttatcttaat tttattggga taaccctttt
      481 gttttttgtt atcataagtt tgtcaatcta ttttaaaagc tatcttcctt ttcaagggaa
      541 attattttta ctttttttgg tagtagatta cgctactttg aatcgttata atatgaaact
      601 gcctatccaa agtaaaaaaa taaatacttt ctatataagg aaaaactata tttttctcta
      661 tcttcttgcc attgtcaaga aatattattc tttccaaatt ttacaatcat tcatttattt
      721 atttattttg tattttctag
//
LOCUS       DAVLFB010000207          687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLFB010000207 DAVLFB010000000
VERSION     DAVLFB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.207"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(29..367)
                     /locus_tag="VXD58_002155"
     CDS             complement(29..367)
                     /locus_tag="VXD58_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676763.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
BASE COUNT          227 a          118 c           81 g          261 t
ORIGIN      
        1 ttgggtctct tgttttcata aattattttt aaaatctata ataatcccaa tatgcattta
       61 agcgaacatc gttccaagat tttgttgcac ttgaatatgc ccatccataa tcttttttat
      121 cctgagcttt aactgttccc caaaaattcg ttactgaaga ttttgatcca atattacttg
      181 gagaactaac aaagtaattc gaataaccat agttatcatt tacaccatat tcccatacat
      241 caataatggg acttggagca gggtgtgccg ttactgcgtt aacgacacta gctgtagtag
      301 caaagctaaa actgaaaaca cttgcaacta gaaaaacaga tgctttctta ttaattcttt
      361 ttttcatttt acttctcctt gaatttaaat atttgatgaa gattaaataa tagtctcttt
      421 ttagtccctc catattactt taatatgaat tgattatatt taggttttta atcaccgtta
      481 tggtatctag agtcgttaaa atatctcata actacccctc cttttaaaat tgaacacatc
      541 taaatgtatc cgtttacatt tttaatgtaa cacttatttt ttaatttgtc aagtatttac
      601 tagtatattt tttattaata aaataggatt atctatacag acaattttcg aatcaacaaa
      661 aaaattatcc aacttgacta gtcacga
//
LOCUS       DAVLFB010000208          683 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLFB010000208 DAVLFB010000000
VERSION     DAVLFB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.208"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            82..561
                     /locus_tag="VXD58_002156"
     CDS             82..561
                     /locus_tag="VXD58_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV0676764.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          266 a           87 c          158 g          172 t
ORIGIN      
        1 ggagctaaag ggaacagaat ggagaaaatg gaacaaatgt gttttctaat ctgttagact
       61 gtatctagaa aggggaaaat tatgattaaa gaattgtatg aagaagtcca agggactgtg
      121 tataagtgta gaaatgaata ttaccttcat ttatgggaat tatcggattg ggaccaagaa
      181 ggcatgctct gcttacatga attgattagt agagaagaag gactggtaga cgatattcca
      241 cgtttaagga aatatttcaa aaccaagttt cgaaatcgaa ttttagacta tatccgtaag
      301 caggaaagtc agaagcgtag atacgataaa gaaccctatg aagaagtggg tgagatcagt
      361 catcgtataa gtgagggggg tctctggcta gatgattatt atctctttca tgaaacacta
      421 agagattata gaaacaaaca aagtaaagag aaacaagaag aactagaacg cgtcttaagc
      481 aatgaacgat ttcgagggcg tcaaagagta ttaagagact tacgcattgt gtttaaggag
      541 tttactatcc gtacccacta gtaagtcatg caaaaaaaaa tgaaaaaaat tagaaaaagt
      601 agttgacaaa atttgaaaag actgtataat agtaagagtt gaaaataaca actcaggtcc
      661 gttggtcaag gggttaagac acc
//
LOCUS       DAVLFB010000209          677 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLFB010000209 DAVLFB010000000
VERSION     DAVLFB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.209"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..241)
                     /locus_tag="VXD58_002157"
     CDS             complement(<1..241)
                     /locus_tag="VXD58_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676765.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFP"
     gene            complement(238..570)
                     /locus_tag="VXD58_002158"
     CDS             complement(238..570)
                     /locus_tag="VXD58_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV0676766.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
BASE COUNT          233 a          133 c           87 g          224 t
ORIGIN      
        1 ctgggaaaaa cttattgatt aaaatgggga aactcattaa cagaatcgaa tttatggcta
       61 gacagataga tactatttta tttttcacag aatatagagg tagtgatact attgagtaga
      121 taaaagctaa aaaaataagc aaaaaatact ccgtgtcatt tataatcgcc ctgaatgata
      181 aaaatatata catccaacat acgataatat gtatttttcg aatcttatct acattattca
      241 tcttactgtc gcagatataa atccatccca aggataaggg ttcccgctaa tatcacaacc
      301 tctattaaga gcaccatatc caatacagta agctattttt cctgctgctg caccaataag
      361 ccagcctcca agacctccaa ccttaatata gtttattagt tttttggtta aaactgcagc
      421 tccagctaat gctataaaag ttcctgaact catagctaaa gctacgcaaa ttccttgaca
      481 agcacgcttg cttaaataag ctcccccatc aagatacatc atttcttctt catcaatcac
      541 aacataatta tttggtaata caagttccat ttgttttctc ctccttagtt gaaattgttt
      601 catttgtttt acaaatagga cgaacttaaa ctatcttttt attactcagt aataatttcc
      661 gagatttcat tttatga
//
LOCUS       DAVLFB010000210          673 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLFB010000210 DAVLFB010000000
VERSION     DAVLFB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.210"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..>673)
                     /locus_tag="VXD58_002159"
     CDS             complement(<1..>673)
                     /locus_tag="VXD58_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV0676767.1"
                     /translation="RKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKIS
                     MTDIAHQLSISTSTVIRKLNDFHFKHDFSRLPEIMSWDEYAFTKGKMSFIAQDFEKLD
                     IITVLEGRTQAIIRNHFLRYDRVVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFH
                     IVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHL
                     TNKEIF"
BASE COUNT          216 a          120 c          135 g          202 t
ORIGIN      
        1 aaaaatctct ttattggtta aatgcatacg aaaagtaggg cgataaaaat gtttatcgct
       61 gagtttacga ctatcctgtt gtatgagctt ccagtagcgc ttgatagcct tgtattcatg
      121 agactttcga tccaattgat tcatgatttg aacacgcaca cgactcatag cacggctaag
      181 atgttgtaca atgtgaaagc gatccaacac gattttagca cacgggaaaa gttgtctagc
      241 taagtcatag taaggactaa acatatccat agtaataatt ttgacgcgac atcggacaac
      301 tctatcgtag cgaagaaagt gatttcgaat gatagcttgt gttctaccct caagaacagt
      361 gatgatatcg agcttttcaa aatcttgcgc aatgaagctc atctttccct ttgtaaaagc
      421 atactcatcc caagacataa tctcaggaag acgcgaaaaa tcatgtttaa agtgaaaatc
      481 attgagctta cgaataacag ttgaagttga gatggaaagc tgatgggcaa tatcagtcat
      541 agaaatcttt tcaattaact tttgagcaat cttttggttg atgatacgag ggatttggtg
      601 attcttcttg acgatagaag tttcagcgac catcattttt gaacagtgat agcacttgaa
      661 tcgacgcttt cta
//
LOCUS       DAVLFB010000211          629 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLFB010000211 DAVLFB010000000
VERSION     DAVLFB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.211"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>629
                     /gene="phtB"
                     /locus_tag="VXD58_002160"
     CDS             <1..>629
                     /gene="phtB"
                     /locus_tag="VXD58_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV0676768.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPH
                     YDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          200 a          129 c          139 g          161 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc
      361 aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg
      421 tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca
      481 ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggccttt atgaggcacc
      541 taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa
      601 cgaacgtccg cattcagata atggttttg
//
LOCUS       DAVLFB010000212          608 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLFB010000212 DAVLFB010000000
VERSION     DAVLFB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.212"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            1..608
                     /locus_tag="VXD58_002161"
                     /pseudo
     CDS             1..608
                     /locus_tag="VXD58_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          203 a          116 c          109 g          180 t
ORIGIN      
        1 ttaaaaacta tcttactgac aatccagacg cttatttgac tgaaatagct tctgaatttg
       61 gctgtcatct aactaccatc cactatgcgc tcaaagctat gggttacact cgaaaaaaag
      121 aaccacacct actatgaaca agacccagaa aaagtagcct tatttcttaa gaattttaat
      181 agtttaaagc acctagcacc tgtttagatt gacgaaacag gattcgatac ttatttttat
      241 cgagaatatg gtcgctcatt aaaaggtcag ttaataagag gcaaagtatc tggaagaaga
      301 tatcagagaa tttctttggt tgcaggtcta acaaatggtg agttaatcgc tccaatgact
      361 tacgaagaga cgatgacgag cgactttttt gaagcttggt ttcagaagtt tctcttacca
      421 acattaacca caccatcggt tattattatg gataatgcaa gattccatag aatgggtaag
      481 ttagaactct tgtgtgaaga gtttgggtat aactttttac ctcttcctcc ctactcacct
      541 gagtacaatc ctattgagaa aacatgggct catatcaaaa agcacctcaa aaaggtatta
      601 ccaagttg
//
LOCUS       DAVLFB010000213          560 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLFB010000213 DAVLFB010000000
VERSION     DAVLFB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.213"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>560
                     /locus_tag="VXD58_002162"
     CDS             <1..>560
                     /locus_tag="VXD58_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV0676769.1"
                     /translation="SNEDLGATNARNIGIKNSRGKYISFLDDDDEYMSDRILKLMACF
                     KKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLE
                     LINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADE
                     EYYNYCRKYFNLLSFNERILVTKKYY"
BASE COUNT          210 a           57 c          113 g          180 t
ORIGIN      
        1 taagtaatga agatttaggt gcaactaatg ccagaaacat aggtatcaaa aattctagag
       61 gtaagtatat atcattttta gacgatgatg atgaatatat gtcagatcga attttaaagt
      121 tgatggcttg ttttaaaaag agtagaatga agaatttagc tttagtttat agttatggaa
      181 taataattta tccaaatggt acacgagaag aggagaagac cgattttgtt ggaaatccct
      241 tgtttgttca aatggttcac aatatagcag gtacgtcatt ttggttgtgt aaaaaagagg
      301 tgctagaatt aattaatggt tttgagaaaa tagattcaca tcaggacggt gttgttttat
      361 taaaactact tgctcaagga taccaaattg atatagtgcg agaattcttg gtgaattact
      421 acgctcacag taaagaaaac ggtatcactg gagtgacaca aaaaacaatt aatgcagatg
      481 aagaatatta taattactgt aggaaatatt ttaatttatt gagtttcaac gagagaatat
      541 tggttacaaa gaaatattat
//
LOCUS       DAVLFB010000214          546 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLFB010000214 DAVLFB010000000
VERSION     DAVLFB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.214"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>546
                     /locus_tag="VXD58_002163"
     CDS             <1..>546
                     /locus_tag="VXD58_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV0676770.1"
                     /translation="EGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKS
                     TLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVK
                     NFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPSSGMD
                     YSNMIKISELINKYKTMDKII"
BASE COUNT          216 a           65 c          105 g          160 t
ORIGIN      
        1 agaaggtgga gagtatcaga taaaaaatct tagctacaaa tttactgatg atgagtgttt
       61 aagcttaaaa gatatttcat tcaagcttgg gaaaatttat ggcataatag gatccaacgg
      121 acgaggaaaa tcaacgcttt taagatgttt aataggtctt gagaaaaaat caaaagaaga
      181 aatttatttt aagggagaga agctatctaa aaaagaaaga ctcaaaaact cttcacttgt
      241 tatgcaagat gtaaatcatc aattattcac agatgaagta ttcaacgagc ttagattagg
      301 agtaaagaat tttgatgaag aaaaggcgaa aatcatttta aaagatttag gcctggacga
      361 atttattgaa aggcatccga tgagtttatc aggagggcaa aagcaaaggc ttgcaatagc
      421 atctgttatg tgcaagaatt ctccatttat cttttttgac gaaccttcaa gtggtatgga
      481 ttattccaat atgataaaaa tatctgaact gattaataag tataaaacca tggataaaat
      541 aatttt
//
LOCUS       DAVLFB010000215          539 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLFB010000215 DAVLFB010000000
VERSION     DAVLFB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.215"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..208
                     /locus_tag="VXD58_002164"
     CDS             <1..208
                     /locus_tag="VXD58_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV0676771.1"
                     /translation="LEDAEKFATLFNSLNIDQVQLLPFHQFGENKYRLLNRKYEMDGI
                     NALHPEDLIDYQKVFLNHHINCYF"
     gene            283..480
                     /locus_tag="VXD58_002165"
     CDS             283..480
                     /locus_tag="VXD58_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV0676772.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          189 a           96 c           75 g          179 t
ORIGIN      
        1 tttagaggat gcagaaaaat tcgctactct atttaactca ttaaatatcg accaagttca
       61 actactccct tttcatcaat ttggtgaaaa caaatatcgt ttattaaatc ggaaatatga
      121 aatggatgga atcaacgcac ttcatcctga agatcttatt gattatcaaa aggtatttct
      181 gaaccaccat attaattgtt atttctagtt tatttccttg aaatgctcta gctatttgca
      241 gataacaagc atctatataa ggttatcaaa gaagacttcc ccatgactct aaatgaatat
      301 atcttacaat atcgcctgaa acaagctata gataagatgg ctgaatctcc caactcccct
      361 ttaagcgcta tctctgacca agttggattt tcagactata aatattttgc caaagtattt
      421 aaaaagtatc tccatatttc cccaaagaaa ttgaaatcac tcggaagaat agtcaaatag
      481 gctattcttc tttttgtaga gaattttact ctaatatagt gaattgaaat aagatgtga
//
LOCUS       DAVLFB010000216          534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLFB010000216 DAVLFB010000000
VERSION     DAVLFB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.216"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..510)
                     /locus_tag="VXD58_002166"
     CDS             complement(<1..510)
                     /locus_tag="VXD58_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV0676773.1"
                     /translation="MVAGQLLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGI
                     IFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLN
                     DQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSL
                     NLTNQNEAFL"
BASE COUNT          195 a           92 c           76 g          171 t
ORIGIN      
        1 taaaaaagct tcattttggt tagttaaatt tagactaact tctcgcattt tcgatgcaaa
       61 gatttttggt acaagtagca taatcattaa tgaaaacaag gtggctacag tcaatgacca
      121 atgatagtga ttaagagtca caactgcaaa tatagtacca gaaattcctt ttattactaa
      181 aaaaagttgt ttaaaagcct gatcatttaa agtctgaaca tcattattta gccacgaaag
      241 atatgttcct gatgatttac tatgaaattc ttgataggta gagttagaga tgtctgtggc
      301 aactctattt cgaatctcta gattaaactc ttggatcact tcaacctgat aatttttcac
      361 tacccagtca aggaatatta tcccacacca gacaatcatt tggtagattg acaatttcaa
      421 aaaccgctct aaattcatcg caattaattc attcaacacc agagcattaa tagttgctgc
      481 ataaattagc aataattgac cagcaacaat aaatatcgtt aataaactaa attt
//
LOCUS       DAVLFB010000217          486 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLFB010000217 DAVLFB010000000
VERSION     DAVLFB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.217"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..343)
                     /locus_tag="VXD58_002167"
     CDS             complement(<1..343)
                     /locus_tag="VXD58_002167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676774.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGM"
BASE COUNT          207 a           79 c           59 g          141 t
ORIGIN      
        1 tcataccttg ataccaaata aaattcggta tttcaataag agcatgaggt aatattcctt
       61 ttattgtagc tactattcca atcatctgac cagatatacc caattgtatt cctaaagata
      121 gcaaatcaaa aagttgcaat aagggcgaaa tcggaaaaca gaatagatat agcaaaaaat
      181 ttgtagaatt tgcctgtaaa actgtcaatg taaattgtaa ttccagtttc ggaggtatag
      241 tagcaaaagt ctcatttttt aatataaagc ttaaaagtaa acctagtaaa aaacagacta
      301 tacaaaataa cacatagtaa atcagaaaac cactttttct catctaatgc cccctactaa
      361 aattacaaat attatcagaa aacaatataa aaccagaaaa ctccatctca ataaactaac
      421 tgtaccttta attaaaaata gagctttctt ataatagaca taggctataa aataattgaa
      481 tggaat
//
LOCUS       DAVLFB010000218          482 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLFB010000218 DAVLFB010000000
VERSION     DAVLFB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.218"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..>482)
                     /locus_tag="VXD58_002168"
     CDS             complement(<1..>482)
                     /locus_tag="VXD58_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV0676775.1"
                     /translation="GQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNYA
                     GPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGSKDV
                     LGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKENITDTAVKALDKINRLRMV
                     A"
BASE COUNT          131 a          141 c           74 g          136 t
ORIGIN      
        1 gcaaccattc ttaatctgtt gattttatcc aaggctttca cagctgtatc tgtgatgttc
       61 tcttttaagc gtctgtctga tttttgttcc atccaatact tcacgctacc gctaccgacc
      121 tgattccacc aaacaaccgc attccttccg cctttgggat tccaaccatc accaagcaca
      181 tctttacttc caagttcgat accatttgaa aacacaggag aacgagaaaa agtagtattc
      241 ccatagaagt ttgctctcga tgaattcgaa aaatccactt gatcataaaa accgacttca
      301 ttcctacagt acattttccc atcagtattg acgttccatg ctttaggtcc ggcatagttc
      361 caattatttc cccagttcgc ccagaaggct gtccggactc catacccggc accattcccc
      421 ataccaacag agaactgatt gacacctgaa atccagcgac cgccaccctg gtcaaattga
      481 cc
//
LOCUS       DAVLFB010000219          435 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVLFB010000219 DAVLFB010000000
VERSION     DAVLFB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.219"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>435
                     /locus_tag="VXD58_002169"
     CDS             <1..>435
                     /locus_tag="VXD58_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV0676776.1"
                     /translation="KANFQVEFLNNLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQG
                     DFESYQDFVLKGGFGFVILKNNELIAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLG
                     AAMILESLNRDMFPLWDAHNEASKKVAEFLGYELSEPYEAFEL"
BASE COUNT          139 a           54 c           95 g          147 t
ORIGIN      
        1 aaggcaaatt ttcaagttga atttttaaat aatctagtta ctcatttaga ggaaggttac
       61 aatattgtgc ctattgataa tcatatttat aactgctttt ctacggaaga atggtcacag
      121 gatttacagg gggattttga gtcctatcag gattttgttt taaaaggtgg atttggcttt
      181 gtgattctta aaaataatga actgattgct gggatttcct cagggttagt ttaccgtaaa
      241 gcagttgaag tggaagttgc aactagacca aacgaacaag gaaatggatt tgctaaaaag
      301 cttggtgctg caatgattct agagagttta aatagagata tgtttccact ttgggatgct
      361 cataatgagg cttccaaaaa agtagcagaa tttttaggat atgagttatc tgaaccttat
      421 gaagcttttg aacta
//
LOCUS       DAVLFB010000220          388 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVLFB010000220 DAVLFB010000000
VERSION     DAVLFB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.220"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            complement(<1..292)
                     /gene="tnpB"
                     /locus_tag="VXD58_002170"
     CDS             complement(<1..292)
                     /gene="tnpB"
                     /locus_tag="VXD58_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV0676777.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWL"
BASE COUNT          124 a           87 c           74 g          103 t
ORIGIN      
        1 taagccaatc tacttgttca ggtgtgagag ctttgacatc cttttctgta ctgggccaag
       61 tcagtctgcc gttctcaaag cgtttatata gtagccaaaa tccttgacca tcccagtaaa
      121 gggctttaaa gcggtcttta cgtccaccac aaaagagaaa aacttgaccg gagaaaggat
      181 ccaattcaaa gtgggtttta accagataag ccagtgaatc gattccttgt ctcatatcag
      241 tcttcccaca cacgagatag acctgcccta ggctagatag atgaattgtc atagagcaac
      301 accttatcca aaagctgttc taccatttcg agattgagag attgaaaaaa acttacttca
      361 agcttgccaa tacgaattta aagcagaa
//
LOCUS       DAVLFB010000221          385 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVLFB010000221 DAVLFB010000000
VERSION     DAVLFB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.221"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            29..385
                     /locus_tag="VXD58_002171"
                     /pseudo
     CDS             29..385
                     /locus_tag="VXD58_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          138 a           46 c           74 g          127 t
ORIGIN      
        1 tattaataaa aaagtaaaga cctattcttt gggtacaaaa caaaaattag ctttgcttct
       61 aactctcgtt acggaacctg atatattgat tttagatgaa ccgactaatg gtttagatat
      121 tgaatcatca caaatagttt tagcggttct aaaaaaaatt agctttaaat gaaaacgtgg
      181 gaattttaat atcgagtcat aaattagaag acattgaaga aatttgtgag agggttcttt
      241 tcttggagag cgggcttttg acatttcaaa aagtaggaaa agatagtcat aatttcttgt
      301 ttgagatagc tttttcatca gctacagata gagacatttt cattaccaaa caagaatttg
      361 gggatattgt tcaggaagag ggatt
//
LOCUS       DAVLFB010000222          382 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVLFB010000222 DAVLFB010000000
VERSION     DAVLFB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.222"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..87
                     /locus_tag="VXD58_002172"
     CDS             <1..87
                     /locus_tag="VXD58_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV0676778.1"
                     /translation="TDEVDGMRFSTFSTNRCGNWSAFSWENC"
BASE COUNT          111 a           46 c           89 g          136 t
ORIGIN      
        1 acagatgaag ttgatggaat gcgatttagt acattcagta caaaccgttg cggtaactgg
       61 agtgctttta gttgggaaaa ttgttaattc atttaaattg taaactttag ggattctctg
      121 aagttatgga tgtcgttcca atatgtgtac gttggaatgt tagtgcttat atggggggaa
      181 cagaatcctc tcttgattga agacaagtta gccattaggc tggtttgtct ctttgtcaac
      241 tgtagtgggt tgatataata gtattagtga gtgggataaa agtttcattt agtttattca
      301 gtacaaattt aacgggtcaa gatttattga actgcacccc aatagttaga cagaaaaaat
      361 ctaacttttg gggtgtttta tt
//
LOCUS       DAVLFB010000223          331 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVLFB010000223 DAVLFB010000000
VERSION     DAVLFB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.223"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>331
                     /locus_tag="VXD58_002173"
     CDS             <1..>331
                     /locus_tag="VXD58_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676779.1"
                     /translation="TFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKD
                     KGLWYYL"
BASE COUNT          125 a           38 c           64 g          104 t
ORIGIN      
        1 atacatttgc aattcctgaa actgttaagt tctatgtaac atcagaacat ataaaagatg
       61 ttcttaaatc aaatttatct actagtaatg atatcattgt tgaaaaagta gataatataa
      121 aacaagaaac tgatgtagct aaacctaaaa agaattctaa tcagggagta gttggttggg
      181 ttaaagacaa aggtttatgg tattacttaa acgaatcagg ttcaatggct actggttggg
      241 ttaaagacaa aggtttatgg tattacttaa acgaatcagg ttcaatggct actggttggg
      301 ttaaagacaa aggcttatgg tattacttaa a
//
LOCUS       DAVLFB010000224          320 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00066702
            SAMN33606766-rid17953113.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVLFB010000224 DAVLFB010000000
VERSION     DAVLFB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606766
            Sequence Read Archive: SRR23726017
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721894.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721894.1
            Genome Coverage       :: 74x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 02:14:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,174
            CDSs (total)                      :: 2,129
            Genes (coding)                    :: 2,010
            CDSs (with protein)               :: 2,010
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 58 of 119
            Pseudo Genes (incomplete)         :: 55 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 28 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606766-rid17953113.denovo.224"
                     /strain="AUSMDU00066702"
                     /serotype="09N"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST13708"
     gene            <1..>320
                     /locus_tag="VXD58_002174"
     CDS             <1..>320
                     /locus_tag="VXD58_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV0676780.1"
                     /translation="GSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIK
                     SKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNLEPDFSSRLYQKVTFSAWIKYENV
                     VQGR"
BASE COUNT          114 a           57 c           73 g           76 t
ORIGIN      
        1 ggctctatcc gtcaggaaat cctgaacgca accaaggata aggcagataa gactttagtt
       61 gtatctgaag ccgggaaatt gcgtgaagaa ttttcaaaaa tgaaggtggg aggtcggaat
      121 ctatggataa aatccaagac ggttggagct gtaattgaaa aattacctga aaaccacgtc
      181 acaggtcaaa aagaatgcta taggctagag aacaactcta ctttaacgtt caaccttgaa
      241 ccagatttca gctcaaggtt gtaccaaaaa gttactttta gcgcttggat caagtacgaa
      301 aatgtagtcc aaggtcgaaa
//
